title: Computational methods for analyzing conformational variability of macromolecular complexes from cryo-electron microscopy images.
abstract: Thanks to latest technical advances in cryo-electron microscopy (cryo-EM), structures of macromolecular complexes (viruses, ribosomes, etc.) are now often obtained at near-atomic resolution. Also, studies of conformational changes of complexes, in connection with their function, are gaining ground. Conformational variability analysis is usually done by classifying images in a number of discrete classes supposedly representing all conformational states present in the specimen. However, discrete classes cannot be meaningfully defined when the conformational change is continuous (the specimen contains a continuum of states instead of a few discrete states). For such cases, first image analysis methods that explicitly consider continuous conformational changes were recently developed. The latest developments in cryo-EM image analysis methods for conformational variability analysis are the focus of this review.
authors.affiliation: IMPMC, Sorbonne Universités-CNRS UMR 7590, UPMC Univ. Paris 6, MNHN, IRD UMR 206, 75005 Paris, France. Electronic address: Slavica.Jonic@impmc.upmc.fr.
authors.forename: Slavica
authors.initials: S
authors.lastname: Jonić
mesh_terms: D020285:Cryoelectron Microscopy / Q000379:methods*; D021621:Imaging, Three-Dimensional; D046911:Macromolecular Substances / Q000737:chemistry*; D008968:Molecular Conformation
chemical_list: D046911:Macromolecular Substances