title: Does caffeine influence the anticholinesterase and antioxidant properties of donepezil? Evidence from in vitro and in vivo studies.
abstract: Caffeine is adjudged world's most consumed pharmacologically active food component. With reports of the potential cognitive enhancing properties of caffeine, we sought to investigate if caffeine can influence the anticholinesterase and antioxidant properties of donepezil-a selective acetylcholinesterase (AChE) inhibitor used in the management of Alzheimer's disease (AD). In vitro, we investigated the effect of donepezil (DON), caffeine (CAF) and their various combinations on the activity of AChE in rat brain homogenate, as well as determined their antioxidant properties. In vivo, two rat groups were administered single oral dose of DON (5 mg/kg) and CAF (5 mg/kg) separately, while three groups, each received 5 mg/kg DON plus either 5, 50 or 100 mg/kg CAF for three hours, after which the rats were sacrificed and brain isolated. Results show that CAF concentration dependently and synergistically increased the anticholinesterase properties of DON in vitro. Also, CAF produced a significant influence on investigated in vitro antioxidant properties of DON. Furthermore, rats administered 5 mg/kg CAF and DON produced no significant difference in AChE activity compared to rats administered DON alone. However, co-administration of either 50 or 100 mg/kg CAF with DON lead to higher AChE activity compared to both control and DON groups. In addition, DON, CAF and their various combinations augmented brain antioxidant status in treated rats. We conclude that while low caffeine consumption may improve the antioxidant properties of donepezil without having a significant influence on its anticholinesterase effect, moderate-high caffeine consumption could also improve the antioxidant properties of donepezil but reduce its anticholinesterase effect; nevertheless, a comprehensive clinical trial is essential to fully explore these possibilities in human AD condition.
authors.affiliation: Department of Biochemistry, Federal University of Technology Akure, P.M.B. 704, Akure, 340001, Nigeria. goboh2001@yahoo.com.|Department of Biochemistry, Federal University Oye, Oye, Ekiti, Nigeria. goboh2001@yahoo.com.
authors.forename: Ganiyu
authors.initials: G
authors.lastname: Oboh
authors.affiliation: Department of Biochemistry, Federal University of Technology Akure, P.M.B. 704, Akure, 340001, Nigeria.|Department of Biomedical Technology, Federal University of Technology Akure, Akure, Nigeria.
authors.forename: Opeyemi Babatunde
authors.initials: OB
authors.lastname: Ogunsuyi
authors.affiliation: Department of Biochemistry, Federal University of Technology Akure, P.M.B. 704, Akure, 340001, Nigeria.|Department of Biochemistry, Federal University Oye, Oye, Ekiti, Nigeria.
authors.forename: Oluwaseyi Emmanuel
authors.initials: OE
authors.lastname: Olonisola
mesh_terms: D000110:Acetylcholinesterase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001923:Brain Chemistry / Q000187:drug effects; D002110:Caffeine / Q000494:pharmacology*; D000697:Central Nervous System Stimulants / Q000494:pharmacology*; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D000077265:Donepezil; D004347:Drug Interactions; D018565:Food-Drug Interactions; D016166:Free Radical Scavengers / Q000494:pharmacology; D066298:In Vitro Techniques; D007189:Indans / Q000494:pharmacology*; D007502:Iron Chelating Agents / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D018697:Nootropic Agents / Q000494:pharmacology*; D010880:Piperidines / Q000494:pharmacology*; D051381:Rats
chemical_list: D000975:Antioxidants; D000697:Central Nervous System Stimulants; D002800:Cholinesterase Inhibitors; D016166:Free Radical Scavengers; D007189:Indans; D007502:Iron Chelating Agents; D018697:Nootropic Agents; D010880:Piperidines; D002110:Caffeine; D000077265:Donepezil; D000110:Acetylcholinesterase
keywords: Anticholinesterase; Antioxidant; Caffeine; Donepezil; Food-drug interaction