title: Phosphorus acquisition efficiency in arbuscular mycorrhizal maize is correlated with the abundance of root-external hyphae and the accumulation of transcripts encoding PHT1 phosphate transporters.
abstract: Plant interactions with arbuscular mycorrhizal fungi have long attracted interest for their potential to promote more efficient use of mineral resources in agriculture. Their use, however, remains limited by a lack of understanding of the processes that determine the outcome of the symbiosis. In this study, the impact of host genotype on growth response to mycorrhizal inoculation was investigated in a panel of diverse maize lines. A panel of 30 maize lines was evaluated with and without inoculation with arbuscular mycorrhizal fungi. The line Oh43 was identified to show superior response and, along with five other reference lines, was characterized in greater detail in a split-compartment system, using33P to quantify mycorrhizal phosphorus uptake. Changes in relative growth indicated variation in host capacity to profit from the symbiosis. Shoot phosphate content, abundance of root-internal and -external fungal structures, mycorrhizal phosphorus uptake, and accumulation of transcripts encoding plant PHT1 family phosphate transporters varied among lines. Superior response in Oh43 is correlated with extensive development of root-external hyphae, accumulation of specific Pht1 transcripts and high phosphorus uptake by mycorrhizal plants. The data indicate that host genetic factors influence fungal growth strategy with an impact on plant performance.
authors.affiliation: Laboratorio Nacional de Genómica para la Biodiversidad (LANGEBIO), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Irapuato, C.P. 36821, Guanajuato, Mexico.|Department of Plant Biology, University of Lausanne, 1014, Lausanne, Switzerland.
authors.forename: Ruairidh J H
authors.initials: RJ
authors.lastname: Sawers
authors.affiliation: Department of Chemical and Biochemical Engineering, Technical University of Denmark, DK-2800, Kgs. Lyngby, Denmark.|Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, DK-1871, Frederiksberg C, Denmark.
authors.forename: Simon F
authors.initials: SF
authors.lastname: Svane
authors.affiliation: Department of Plant Sciences, University Cambridge, Cambridge, CB2 3EA, UK.
authors.forename: Clement
authors.initials: C
authors.lastname: Quan
authors.affiliation: Department of Chemical and Biochemical Engineering, Technical University of Denmark, DK-2800, Kgs. Lyngby, Denmark.|Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, DK-1871, Frederiksberg C, Denmark.
authors.forename: Mette
authors.initials: M
authors.lastname: Grønlund
authors.affiliation: Department of Plant Biology, University of Lausanne, 1014, Lausanne, Switzerland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Wozniak
authors.affiliation: Department of Plant Biology, University of Lausanne, 1014, Lausanne, Switzerland.
authors.forename: Mesfin-Nigussie
authors.initials: MN
authors.lastname: Gebreselassie
authors.affiliation: Laboratorio Nacional de Genómica para la Biodiversidad (LANGEBIO), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Irapuato, C.P. 36821, Guanajuato, Mexico.
authors.forename: Eliécer
authors.initials: E
authors.lastname: González-Muñoz
authors.affiliation: Laboratorio Nacional de Genómica para la Biodiversidad (LANGEBIO), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Irapuato, C.P. 36821, Guanajuato, Mexico.
authors.forename: Ricardo A
authors.identifier: http://orcid.org/0000-0001-6132-8956
authors.initials: RA
authors.lastname: Chávez Montes
authors.affiliation: USDA-ARS, Donald Danforth Plant Science Center, St Louis, MO, 63132, USA.
authors.forename: Ivan
authors.initials: I
authors.lastname: Baxter
authors.affiliation: Department of Ecology and Evolution, University of Lausanne, 1014, Lausanne, Switzerland.
authors.forename: Jerome
authors.initials: J
authors.lastname: Goudet
authors.affiliation: Department of Chemical and Biochemical Engineering, Technical University of Denmark, DK-2800, Kgs. Lyngby, Denmark.|Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, DK-1871, Frederiksberg C, Denmark.
authors.forename: Iver
authors.initials: I
authors.lastname: Jakobsen
authors.affiliation: Department of Plant Biology, University of Lausanne, 1014, Lausanne, Switzerland.|Department of Plant Sciences, University Cambridge, Cambridge, CB2 3EA, UK.
authors.forename: Uta
authors.initials: U
authors.lastname: Paszkowski
mesh_terms: D018533:Biomass; D018506:Gene Expression Regulation, Plant; D025301:Hyphae / Q000378:metabolism*; D038821:Mycorrhizae / Q000378:metabolism*; D028061:Phosphate Transport Proteins / Q000235:genetics* / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism*; D063245:Plant Development; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000382:microbiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D003313:Zea mays / Q000235:genetics* / Q000382:microbiology*
chemical_list: D028061:Phosphate Transport Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D010758:Phosphorus
keywords: PHT1; arbuscular mycorrhiza (AM); maize; phosphorus; root-external hyphae