title: Highly sensitive aptasensor based on synergetic catalysis activity of MoS2-Au-HE composite using cDNA-Au-GOD for signal amplification.
abstract: Single or few-layer nanosheets of MoS2(MoS2nanosheets) and a composite composed of MoS2nanosheets, Au nanoparticles (AuNPs) and hemin (HE) (denoted as MoS2-Au-HE) were prepared. The composites possessed high synergetic catalysis activity towards the electroreduction of hydrogen peroxide. Furthermore, glucose oxidase (GOD) and AuNPs were used as marker of the complementary DNA (cDNA) strand of kanamycin aptamer to prepare a conjugate (reffered as cDNA-Au-GOD) that was designed as the signal probe. Both cDNA-Au-GOD and MoS2-Au-HE were applied to fabricate aptasensor for kanamycin. MoS2-Au-HE acted as solid platform for kanamycin aptamer and signal transmitters. AuNPs were employed as the supporter of cDNA and GOD which catalyze dissolved oxygen to produce hydrogen peroxide in the presence of glucose. Then cathodic peak current of H2O2was recorded by differential pulse voltammetry (DPV). The electrochemical reduction of H2O2was catalyzed by MoS2-Au-HE that was modified onto the surface of a glassy carbon electrode (GCE). The cathodic peak current of H2O2was highly linearly decreased with an increase of kanamycin concentrations from 1.0ng/L to 1.0×105ng/L, with a detection limit of 0.8ng/L. This aptasensor can be used to detect kanamycin in milk with high specificity, sensitivity and selectivity.
authors.affiliation: Key Laboratory of Beijing on Regional Air Pollution Control, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Song
authors.affiliation: Key Laboratory of Beijing on Regional Air Pollution Control, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, PR China. Electronic address: kangtf@bjut.edu.cn.
authors.forename: Tian-Fang
authors.initials: TF
authors.lastname: Kang
authors.affiliation: Key Laboratory of Beijing on Regional Air Pollution Control, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Lu
authors.affiliation: Key Laboratory of Beijing on Regional Air Pollution Control, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Shui-Yuan
authors.initials: SY
authors.lastname: Cheng
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000378:metabolism; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D018076:DNA, Complementary / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D006046:Gold / Q000737:chemistry*; D006427:Hemin / Q000737:chemistry*; D007612:Kanamycin / Q000032:analysis; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D008892:Milk / Q000737:chemistry; D008982:Molybdenum / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D018076:DNA, Complementary; D004220:Disulfides; D007612:Kanamycin; D006427:Hemin; D006046:Gold; D008982:Molybdenum; D005949:Glucose Oxidase; C082964:molybdenum disulfide
keywords: Aptasensor; Glucose oxidase; Hemin; Kanamycin; Molybdenum disulfide