title: Comparison of mucoadhesive and cohesive features of poly(acrylic acid)-conjugates respective their molecular mass.
abstract: OBJECTIVE
  This study aimed to assess the impact of molecular mass as well as the differences between poly(acrylic acid)-thiol-conjugates (PAA100,250,450KDa) on their mucoadhesive and cohesive qualities.
  
  
  METHODS
  Covalent attachment of cysteine (CYS), cysteamine (CYSM) and l-gluthathione (GSH) to poly(acrylic acid) was achieved by formation of amide bonds between primary amino group of the amino acid (in the case of cysteine and glutathione), respectively the amino group of the aminothiol cysteamine and carboxylic acid group of the polymer. Obtained polymer conjugates were evaluated in regard to their safety profile, mucoadhesive properties on the buccal mucosa by rotating cylinder, tensile strength and rheological investigations, respectively. Furthermore, stability, cohesive and water uptake studies were performed.
  
  
  RESULTS
  Mucoadhesive studies revealed that maximum detachment force of PAACYS450 was 24.3-fold higher in comparison to the respective controls. Stability studies revealed for PAACYS450 a 50.2-fold higher stability compared to controls.
  
  
  CONCLUSIONS
  Taken together, among all polymers tested, PAACYS450 evinced the most favorable qualities regarding mucoadhesion and cohesion, followed by PAACYSM450 and PAACYS250.
authors.affiliation: Department of Pharmaceutical Technology, Institute of Pharmacy, Center for Molecular Biosciences Innsbruck, University of Innsbruck, Austria. Electronic address: Flavia.Laffleur@uibk.ac.at.
authors.forename: Flavia
authors.initials: F
authors.lastname: Laffleur
authors.affiliation: Department of Pharmaceutical Technology, Institute of Pharmacy, Center for Molecular Biosciences Innsbruck, University of Innsbruck, Austria.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Knapp
authors.affiliation: Department of Pharmaceutical Technology, Institute of Pharmacy, Center for Molecular Biosciences Innsbruck, University of Innsbruck, Austria.
authors.forename: Wongsakorn
authors.initials: W
authors.lastname: Suchaoin
authors.affiliation: Department of Pharmaceutical Technology, Institute of Pharmacy, Center for Molecular Biosciences Innsbruck, University of Innsbruck, Austria.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Partenhauser
authors.affiliation: Department of Pharmaceutical Technology, Institute of Pharmacy, Center for Molecular Biosciences Innsbruck, University of Innsbruck, Austria; Faculty of Pharmacy, Thammasat University, Rungsit Campus, Phahonyothin Road, Khlong Luang, Pathumthani 12120, Thailand.
authors.forename: Kesinee
authors.initials: K
authors.lastname: Netsomboon
authors.affiliation: Department of Pharmaceutical Technology, Institute of Pharmacy, Center for Molecular Biosciences Innsbruck, University of Innsbruck, Austria.
authors.forename: Andreas
authors.initials: A
authors.lastname: Bernkop-Schnürch
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D018938:Caco-2 Cells; D006801:Humans; D008970:Molecular Weight; D009092:Mucous Membrane; D012212:Rheology; D013718:Tensile Strength; D014014:Tissue Adhesives
chemical_list: D000180:Acrylic Resins; D014014:Tissue Adhesives; C006903:carbopol 940
keywords: Buccal delivery; Mucoadhesive; Poly(acrylic acid); Resazurin assay; Rheology