title: Anillin Phosphorylation Controls Timely Membrane Association and Successful Cytokinesis.
abstract: During cytokinesis, a contractile ring generates the constricting force to divide a cell into two daughters. This ring is composed of filamentous actin and the motor protein myosin, along with additional structural and regulatory proteins, including anillin. Anillin is a required scaffold protein that links the actomyosin ring to membrane and its organizer, RhoA. However, the molecular basis for timely action of anillin at cytokinesis remains obscure. Here, we find that phosphorylation regulates efficient recruitment of human anillin to the equatorial membrane. Anillin is highly phosphorylated in mitosis, and is a substrate for mitotic kinases. We surveyed function of 46 residues on anillin previously found to be phosphorylated in human cells to identify those required for cytokinesis. Among these sites, we identified S635 as a key site mediating cytokinesis. Preventing S635 phosphorylation adjacent to the AH domain disrupts anillin concentration at the equatorial cortex at anaphase, whereas a phosphomimetic mutant, S635D, partially restores this localization. Time-lapse videomicroscopy reveals impaired recruitment of S635A anillin to equatorial membrane and a transient unstable furrow followed by ultimate failure in cytokinesis. A phosphospecific antibody confirms phosphorylation at S635 in late cytokinesis, although it does not detect phosphorylation in early cytokinesis, possibly due to adjacent Y634 phosphorylation. Together, these findings reveal that anillin recruitment to the equatorial cortex at anaphase onset is enhanced by phosphorylation and promotes successful cytokinesis.
authors.affiliation: University of Wisconsin Carbone Cancer Center and Department of Medicine, Hematology/Oncology, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.
authors.forename: Hyunjung
authors.initials: H
authors.lastname: Kim
authors.affiliation: University of Wisconsin Carbone Cancer Center and Department of Medicine, Hematology/Oncology, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.
authors.forename: James M
authors.initials: JM
authors.lastname: Johnson
authors.affiliation: University of Wisconsin Carbone Cancer Center and Department of Medicine, Hematology/Oncology, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.
authors.forename: Robert F
authors.identifier: http://orcid.org/0000-0001-8713-4279
authors.initials: RF
authors.lastname: Lera
authors.affiliation: University of Wisconsin Carbone Cancer Center and Department of Medicine, Hematology/Oncology, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.
authors.forename: Sarang
authors.initials: S
authors.lastname: Brahma
authors.affiliation: University of Wisconsin Carbone Cancer Center and Department of Medicine, Hematology/Oncology, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.
authors.forename: Mark E
authors.initials: ME
authors.lastname: Burkard
mesh_terms: D002462:Cell Membrane / Q000378:metabolism*; D048749:Cytokinesis; D006367:HeLa Cells; D006801:Humans; D008840:Microfilament Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational
chemical_list: C000591059:ANLN protein, human; D008840:Microfilament Proteins