title: The cardioprotective effect of total flavonoids on myocardial ischemia/reperfusion in rats.
abstract: The flowers of Abelmoschus manihot (L.) Medic is a traditional Chinese medicine used for the treatment of ischemic diseases. The present study is to investigate whether total flavones (TA) of extracted from Abelmoschus manihot L. Medic has the potential cardioprotective effect on myocardial ischemia/reperfusion (I/R) damage in rats. The index of myocardial injury, inflammatory biomarkers and NLRP3-related parameters were measured, respectively. The results demonstrated that compared to I/R group, TA reduced myocardial infarction area, declined serum creatinine kinase (CK), lactate dehydrogenase (LDH) levels, attenuated serum interleukin-6 (IL-6), IL-1β and tumour necrosis factor (TNF-α) production. Moreover, TA markedly enhanced the activities of superoxide dismutase (SOD) and reduced the amounts of malondialdehyde (MDA) in I/R rats. In addition, TA reduced myocardial I/R induced injury in rats by inhibiting NLRPR3 inflammasome. Thus, it is assumed that TA could significantly improve myocardial I/R injury in rats partially through suppressing NLRP3 activtion.
authors.affiliation: Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210009, China.
authors.forename: Dongling
authors.initials: D
authors.lastname: Lv
authors.affiliation: Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210009, China.
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210009, China.
authors.forename: Lingyi
authors.initials: L
authors.lastname: Tang
authors.affiliation: Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210009, China,. Electronic address: jmjsszyy@sina.com.
authors.forename: Meng
authors.initials: M
authors.lastname: Jiang
mesh_terms: D028483:Abelmoschus / Q000737:chemistry; D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology*; D003402:Creatine Kinase / Q000097:blood; D016207:Cytokines / Q000378:metabolism; D004562:Electrocardiography / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology*; D035264:Flowers / Q000737:chemistry; D058847:Inflammasomes / Q000187:drug effects; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002316:Cardiotonic Agents; D016207:Cytokines; D005419:Flavonoids; D058847:Inflammasomes; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C573346:Nlrp3 protein, rat; D010936:Plant Extracts; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase
keywords: Abelmoschus manihot L. Medic; Inflammation; Ischemia/reperfusion (I/R); NLRP3