title: Tubulin Binding and Polymerization Promoting Properties of Tubulin Polymerization Promoting Proteins Are Evolutionarily Conserved.
abstract: Tubulin polymerization promoting proteins (TPPPs) constitute a eukaryotic protein family. There are three TPPP paralogs in the human genome, denoted as TPPP1-TPPP3. TPPP1 and TPPP3 are intrinsically unstructured proteins (IUPs) that bind and polymerize tubulin and stabilize microtubules, but TPPP2 does not. Vertebrate TPPPs originated from the ancient invertebrate TPPP by two-round whole-genome duplication; thus, whether the tubulin/microtubule binding function of TPPP1 and TPPP3 is a newly acquired property or was present in the invertebrate orthologs (generally one TPPP per species) has been an open question. To answer this question, we investigated a TPPP from a simple and early branching animal, the sponge Suberites domuncula. Bioinformatics, biochemical, immunochemical, spectroscopic, and electron microscopic data showed that the properties of the sponge protein correspond to those of TPPP1; namely, it is an IUP that strongly binds tubulin and induces its polymerization, proving that these features of animal TPPPs have been evolutionarily conserved.
authors.affiliation: Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences , Magyar tudósok körútja 2, Budapest H-1117, Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: Oláh
authors.affiliation: Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences , Magyar tudósok körútja 2, Budapest H-1117, Hungary.
authors.forename: Tibor
authors.initials: T
authors.lastname: Szénási
authors.affiliation: Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences , Magyar tudósok körútja 2, Budapest H-1117, Hungary.
authors.forename: Adél
authors.initials: A
authors.lastname: Szabó
authors.affiliation: Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences , Magyar tudósok körútja 2, Budapest H-1117, Hungary.
authors.forename: Kinga
authors.initials: K
authors.lastname: Kovács
authors.affiliation: Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University , Pázmány Péter sétány 1/C, Budapest H-1117, Hungary.
authors.forename: Péter
authors.identifier: 0000-0003-2450-7087
authors.initials: P
authors.lastname: Lőw
authors.affiliation: Department of Natural and Health Studies, Juraj Dobrila University of Pula , Zagrebačka 30, HR-52100 Pula, Croatia.
authors.forename: Mauro
authors.initials: M
authors.lastname: Štifanić
authors.affiliation: Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences , Magyar tudósok körútja 2, Budapest H-1117, Hungary.
authors.forename: Ferenc
authors.identifier: 0000-0003-3169-0078
authors.initials: F
authors.lastname: Orosz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002942:Circular Dichroism; D017124:Conserved Sequence; D019143:Evolution, Molecular; D008854:Microscopy, Electron; D008870:Microtubules / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D047233:Suberites / Q000737:chemistry*; D014404:Tubulin / Q000378:metabolism*
chemical_list: D009419:Nerve Tissue Proteins; D011506:Proteins; C118136:TPPP protein, human; D014404:Tubulin