title: Evaluation of hemoglobin A1cmeasurement from filter paper using high-performance liquid chromatography and immunoturbidimetric assay.
abstract: Glycated hemoglobin (HbA1c) measurement from whole blood (WB) samples is inconvenient for epidemic surveillance and self-monitoring of glycemic level. We evaluated HbA1cmeasurement from WB blotted on filter paper (FP), which can be easily transported to central laboratories, with high-performance liquid chromatography (HPLC) and immunoturbidimetric assay (ITA). WB was applied to Whatman filter paper. By using HPLC and WB samples as reference methods, these FP samples were evaluated on HPLC and ITA. Inter- and intra-assay variation, WB vs. FP agreement and sample stability at 20-25 °C and -70 °C were assessed by statistical analysis. Results showed that the coefficient of variation (CV, %) of FP samples for HPLC and ITA were 0.44-1.02% and 1.47-2.72%, respectively (intra-assay); 2.13-3.56% and 3.21-4.82%, respectively (inter-assay). The correlation of WB HPLC with FP analyzed using HPLC and ITA are both significant (p < 0.001). Sample stability showed that FP method up to 5 days at 20-25 °C and 5 weeks at -70 °C is accurate and reproducible. In conclusion, FP samples analyzed by HPLC and ITA can both provide an alternative to WB for HbA1cmeasurement, supporting the use of FP method in epidemic surveillance and healthcare units.
authors.affiliation: a Department of Laboratory Medicine , Peking University Third Hospital , Beijing , China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Wu
authors.affiliation: a Department of Laboratory Medicine , Peking University Third Hospital , Beijing , China.
authors.forename: Xu
authors.initials: X
authors.lastname: Yang
authors.affiliation: b Department of Endocrinology , Peking University Third Hospital , Beijing , China.
authors.forename: Haining
authors.initials: H
authors.lastname: Wang
authors.affiliation: a Department of Laboratory Medicine , Peking University Third Hospital , Beijing , China.
authors.forename: Zhenrong
authors.initials: Z
authors.lastname: Li
authors.affiliation: a Department of Laboratory Medicine , Peking University Third Hospital , Beijing , China.
authors.forename: Tiancheng
authors.initials: T
authors.lastname: Wang
mesh_terms: D000704:Analysis of Variance; D002851:Chromatography, High Pressure Liquid / Q000592:standards*; D059788:Dried Blood Spot Testing / Q000379:methods / Q000592:standards*; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000592:standards*; D009391:Nephelometry and Turbidimetry / Q000592:standards*; D015588:Observer Variation; D010209:Paper; D015203:Reproducibility of Results
chemical_list: D006442:Glycated Hemoglobin; C517652:hemoglobin A1c protein, human
keywords: Diabetes; HbA1c; filter paper; high-performance liquid chromatography; immunoturbidometric assay