title: Isomelezitose formation by glucansucrases.
abstract: Several glucansucrases were surveyed for their ability to produce isomelezitose, a trisaccharide with the structure α-D-glucopyranosyl (1 → 6) β-D-fructofuranosyl (2 ↔ 1) α-D-glucopyranoside. Nearly all strains tested, with one exception, produced at least trace levels of isomelezitose. Yields were low but significant, ranging from less than 1% to approximately 5% based on sucrose. This trisaccharide may arise in either of two ways: glucopyranosyl transfer to the 6Fru-OH position of sucrose, or to the anomeric OH position of isomaltulose. This study indicates that isomelezitose formation may be a general phenomenon of many glucansucrase reactions.
authors.affiliation: Renewable Product Technology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, 1815 North University Street, Peoria, IL 61604, USA. Electronic address: greg.cote@ars.usda.gov.
authors.forename: Gregory L
authors.initials: GL
authors.lastname: Côté
authors.affiliation: Renewable Product Technology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, 1815 North University Street, Peoria, IL 61604, USA.
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Skory
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D055162:Biocatalysis; D002240:Carbohydrate Sequence; D016695:Glycosyltransferases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D007536:Isomerism; D007778:Lactobacillus / Q000737:chemistry / Q000201:enzymology; D007934:Leuconostoc / Q000737:chemistry / Q000201:enzymology; D000070007:Leuconostoc mesenteroides / Q000737:chemistry / Q000201:enzymology; D009682:Magnetic Resonance Spectroscopy; D013379:Substrate Specificity; D013395:Sucrose / Q000737:chemistry*; D014312:Trisaccharides / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D014312:Trisaccharides; D013395:Sucrose; D016695:Glycosyltransferases; C033837:alternansucrase; C005190:melezitose
keywords: Acceptor reaction; Glucansucrase; Glucosyltransferase; Isomelezitose; Oligosaccharide