title: The genome sequence of a novel RNA virus in Adelphocoris suturalis.
abstract: The complete genome of a novel virus found in Adelphocoris suturalis was determined by RNA-seq and named Adelphocoris suturalis-associated virus 1 (ASV1). ASV1 has a single-stranded RNA genome of 10,845 nucleotides in length and contains five putative open reading frames (ORFs). ORF1 encodes a polypeptide of 2592 amino acids (aa) and contains four conserved domains: a viral RNA methyltransferase domain, an FtsJ-like methyltransferase domain, a viral RNA helicase domain and an RNA-dependent RNA polymerase domain. ORF2, ORF3, ORF4 and ORF5 encode polypeptides of 190, 461, 103 and 159 aa, respectively, of which only ORF5 contains a conserved domain, the Tobacco mosaic virus-coat superfamily. Phylogenetic analysis with the deduced amino acid sequences indicated that ASV1 clusters with the Drosophila-related Boutonnet virus. The similar genomic structure and high bootstrap value identified in the maximum-likelihood tree suggest that ASV1 (possibly alongside Boutonnet virus) could be considered the prototype of a new taxon of unclassified insect viruses. The prevalence of ASV1 infection in wild populations of A. suturalis was at a low level (6.60%, 14 positives from 212 samples).
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, People's Republic of China.|Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors.forename: Xinlian
authors.initials: X
authors.lastname: Li
authors.affiliation: Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, People's Republic of China.
authors.forename: Pengjun
authors.initials: P
authors.lastname: Xu
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, People's Republic of China.
authors.forename: Xianming
authors.initials: X
authors.lastname: Yang
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, People's Republic of China.
authors.forename: He
authors.initials: H
authors.lastname: Yuan
authors.affiliation: Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China. lzchen@mail.hzau.edu.cn.
authors.forename: Lizhen
authors.initials: L
authors.lastname: Chen
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, People's Republic of China. yhlu@ippcaas.cn.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Lu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016679:Genome, Viral / Q000235:genetics*; D020062:Heteroptera / Q000821:virology*; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D020365:RNA Helicases / Q000235:genetics; D012328:RNA Viruses / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA; D012359:tRNA Methyltransferases / Q000235:genetics
chemical_list: D012367:RNA, Viral; D012359:tRNA Methyltransferases; D012324:RNA-Dependent RNA Polymerase; D020365:RNA Helicases