title: A highly sensitive quantum dots-DNA nanobiosensor based on fluorescence resonance energy transfer for rapid detection of nanomolar amounts of human papillomavirus 18.
abstract: A very sensitive and convenient nanobiosensor based on fluorescence resonance energy transfer (FRET) was developed for the detection of a 22-mer oligonucleotides sequence in Human Papillomavirus 18 virus (HPV18) gene. For this purpose, water-soluble CdTe quantum dots (QDs) were synthesized and, subsequently, amino-modified 11-mer oligonucleotide as one of the two necessary probes was attached to QDs surface to form functional QDs-DNA conjugates. Right after addition of the QDs-DNA and a second Cyanine5 (Cy5)-labeled 11-mer oligonucleotide probe to the DNA target solution, the sandwiched hybrids were formed. The resulting hybridization brings the Cy5 fluorophore as the acceptor to close proximity of the QDs as donor, so that an effective transfer of energy from the excited QDs to the Cy5 probe would occur via FRET processing. The fluorescence intensity of Cy5 found to linearly enhance by increasing the DNA target concentration from 1.0 to 50.0nM, with a detection limit of 0.2nM. This homogeneous DNA detection method does not require excessive washing and separation steps of un-hybridized DNA, due to the fact that no FRET can be observed when the probes are not ligated. Finally, feasibility and selectivity of the proposed one-spot DNA detection nanobiosensor were investigated by analysis of derived nucleotides from HPV18 and mismatched sequences.
authors.affiliation: Department of Chemistry, Razi University, Kermanshah, Iran. Electronic address: mshamsipur@yahoo.com.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Shamsipur
authors.affiliation: Department of Chemistry, Razi University, Kermanshah, Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Nasirian
authors.affiliation: Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Kamran
authors.initials: K
authors.lastname: Mansouri
authors.affiliation: Department of Chemistry, Razi University, Kermanshah, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Barati
authors.affiliation: Department of Biochemistry, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Asad
authors.initials: A
authors.lastname: Veisi-Raygani
authors.affiliation: Department of Chemistry, Razi University, Kermanshah, Iran.
authors.forename: Soheila
authors.initials: S
authors.lastname: Kashanian
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D019187:Cadmium Compounds / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation / Q000379:methods*; D052161:Human papillomavirus 18 / Q000235:genetics*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013691:Tellurium / Q000737:chemistry; D013864:Thioglycolates / Q000737:chemistry
chemical_list: D019187:Cadmium Compounds; D004279:DNA, Viral; D009841:Oligonucleotides; D013864:Thioglycolates; C017487:2-mercaptoacetate; D013691:Tellurium; C028337:cadmium telluride
keywords: DNA; Fluorescence resonance energy transfer; Human papillomavirus 18; Nanobiosensor; Quantum dots