title: Dynamic interplay between microbial denitrification and antibiotic resistance under enhanced anoxic denitrification condition in soil.
abstract: Mixed contamination of nitrate and antibiotics/antibiotic-resistant genes (ARGs) is an emerging environmental risk to farmland soil. This is the first study to explore the role of excessive anthropogenic nitrate input in the anoxic dissipation of soil antibiotic/ARGs. During the initial 10 days of incubation, the presence of soil antibiotics significantly inhibited NO3-dissipation, N2O production rate, and denitrifying genes (DNGs) abundance in soil (p < 0.05). Between days 10 and 30, by contrast, enhanced denitrification clearly prompted the decline in antibiotic contents and ARG abundance. Significantly negative correlations were detected between DNGs and ARGs, suggesting that the higher the DNG activity, the more dramatic is the denitrification and the greater are the antibiotic dissipation and ARG abundance. This study provides crucial knowledge for understanding the mutual interaction between soil DNGs and ARGs in the enhanced anoxic denitrification condition.
authors.affiliation: Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China; Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China. Electronic address: sunmingming@njau.edu.cn.
authors.forename: Mingming
authors.initials: M
authors.lastname: Sun
authors.affiliation: Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
authors.forename: Mao
authors.initials: M
authors.lastname: Ye
authors.affiliation: Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Kuan
authors.initials: K
authors.lastname: Liu
authors.affiliation: Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 88743, USA.
authors.forename: Arthur P
authors.initials: AP
authors.lastname: Schwab
authors.affiliation: Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Manqiang
authors.initials: M
authors.lastname: Liu
authors.affiliation: Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Jiaguo
authors.initials: J
authors.lastname: Jiao
authors.affiliation: Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Nanjing Institute of Environmental Science, Ministry of Environmental Protection of China, Nanjing 210042, China.
authors.forename: Jinzhong
authors.initials: J
authors.lastname: Wan
authors.affiliation: Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Da
authors.initials: D
authors.lastname: Tian
authors.affiliation: Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.affiliation: Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Huixin
authors.initials: H
authors.lastname: Li
authors.affiliation: Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: fenghu@njau.edu.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Hu
authors.affiliation: Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D058440:Denitrification; D004352:Drug Resistance, Microbial / Q000235:genetics; D004777:Environment; D005798:Genes, Bacterial; D009566:Nitrates / Q000032:analysis* / Q000378:metabolism; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D009566:Nitrates; D012987:Soil
keywords: Antibiotic-resistant genes; Denitrifiers; Denitrifying genes; Enhanced anoxic denitrification; Mutual dynamic interplay