title: Determination the Role of Rhipicephalus sanguineus for Transmission of Leishmania major to Reservoir Animals.
abstract: OBJECTIVE
  This study aimed to explore the role of Rhipicephalus sanguineus in the transmission of Leishmania major, the etiological agent of zoonotic cutaneous leishmaniasis.
  
  
  METHODS
  Ten gerbils (Meriones unguiculatus) were infected with promastigotes of L. major, and 10 gerbils were maintained as controls. In a controlled environment, 2000 R. sanguineus larvae were fed to two gerbils. Following feeding to gerbils, 65 tick pools were prepared from the engorged larvae and molted unfed nymphs. These pools were tested for the presence of L. major using polymerase chain reaction and real time (RT) PCR.
  
  
  RESULTS
  One of the infected gerbil was anesthetized and necropsied following the dropping of all fed larvae. Following the examination, amastigotes were detected in all organs and tissues. PCR and RT-PCR were performed to test whether the engorged R. sanguineus larvae successfully took the parasite while feeding and was able to transmit it to the next nymphal stage; however, none of the tick pools were found to be positive for L. major.
  
  
  CONCLUSIONS
  Although L.major was not detected in ticks that fed on gerbils, using dogs in experimental studies related to leishmaniasis will give clearer results in terms of detecting the potential role of insects and acars.
authors.affiliation: Adnan Menderes Üniversitesi Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Aydın, Türkiye. hbilgic@adu.edu.tr.
authors.forename: Hüseyin Bilgin
authors.initials: HB
authors.lastname: Bilgiç
authors.forename: Serkan
authors.initials: S
authors.lastname: Bakırcı
authors.forename: Onur
authors.initials: O
authors.lastname: Köse
authors.forename: Ayça
authors.initials: A
authors.lastname: Aksulu
authors.forename: Selin
authors.initials: S
authors.lastname: Hacılarlıoğlu
authors.forename: Tülin
authors.initials: T
authors.lastname: Karagenç
mesh_terms: D000818:Animals; D001096:Arachnid Vectors / Q000469:parasitology*; D004195:Disease Models, Animal; D004197:Disease Reservoirs / Q000469:parasitology; D004283:Dog Diseases / Q000469:parasitology / Q000635:transmission*; D004285:Dogs; D005849:Gerbillinae / Q000469:parasitology*; D006801:Humans; D018320:Leishmania major / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000635:transmission / Q000662:veterinary*; D008297:Male; D016133:Polymerase Chain Reaction; D048508:Rhipicephalus sanguineus / Q000469:parasitology*; D015047:Zoonoses / Q000635:transmission