title: Demethoxycurcumin Preserves Renovascular Function by Downregulating COX-2 Expression in Hypertension.
abstract: Hypertension-associated endothelial dysfunction is largely due to the exaggerated vasoconstrictor generation by cyclooxygenase-2 (COX-2). COX-2 is induced under inflammatory condition. Demethoxycurcumin (DMC) is a major component ofCurcuma longaL, which possesses anti-inflammatory action. This study aimed to examine whether DMC protects endothelial function in hypertension by modulating COX-2. Changes in isometric tension showed that in vivo and ex vivo treatment with DMC rescued the attenuated endothelium-dependent relaxations (EDRs) and elevated endothelium-dependent contractions (EDCs) in the renal arteries of SHR, which were also corrected by acute usage of the COX-2 inhibitor celecoxib. The restoration of renovascular activity by DMC was accompanied by the normalization of COX-2 expression. The enhanced COX-2 expression observed in the renal arteries of hypertensive patients was suppressed by incubation of excised arteries with DMC for 12 hrs. In the renal arteries of Wistar-Kyoto rats (WKY), DMC prevented the endothelial dysfunction caused by angiotensin II. The reduction in the generation of nitric oxide (NO) and expression of eNOS phosphorylation (Ser1177) in human umbilical vein endothelial cells caused by angiotensin II (Ang II) were restored by DMC or celecoxib. Our findings suggest that DMC may decrease COX-2 expression and improve endothelial function in hypertension.
authors.affiliation: Department of Geriatrics, Zhongda Hospital, Medical School, Southeast University, 87# Ding Jiaqiao, Nanjing 210009, China.
authors.forename: Yingjuan
authors.identifier: 0000-0002-5580-1003
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Physiology, Hebei Medical University, Shi Jiazhuang 050017, China.
authors.forename: Danyang
authors.identifier: 0000-0002-4553-0115
authors.initials: D
authors.lastname: Tian
authors.affiliation: Department of Neurology, Second Hospital of Hebei Medical University, Shi Jiazhuang 050017, China.
authors.forename: Chunhua
authors.identifier: 0000-0002-6302-4267
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Department of Geriatrics, Zhongda Hospital, Medical School, Southeast University, 87# Ding Jiaqiao, Nanjing 210009, China.
authors.forename: Liqun
authors.identifier: 0000-0002-1132-8613
authors.initials: L
authors.lastname: Ren
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000368:Aged; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D000068579:Celecoxib / Q000494:pharmacology; D030024:Curcuma / Q000737:chemistry / Q000378:metabolism; D003474:Curcumin / Q000031:analogs & derivatives* / Q000494:pharmacology; D051546:Cyclooxygenase 2 / Q000737:chemistry / Q000378:metabolism*; D036381:Diarylheptanoids; D015536:Down-Regulation / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D006973:Hypertension / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D009569:Nitric Oxide / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012077:Renal Artery / Q000187:drug effects* / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects; D014661:Vasoconstriction / Q000187:drug effects
chemical_list: D036381:Diarylheptanoids; D000804:Angiotensin II; D009569:Nitric Oxide; D052250:Nitric Oxide Synthase Type III; D051546:Cyclooxygenase 2; D003474:Curcumin; D000068579:Celecoxib; D000109:Acetylcholine; C050229:demethoxycurcumin