title: Long non-coding RNA Linc-RAM enhances myogenic differentiation by interacting with MyoD.
abstract: Long non-coding RNAs (lncRNAs) are important regulators of diverse biological processes. Here we report on functional identification and characterization of a novel long intergenic non-coding RNA with MyoD-regulated and skeletal muscle-restricted expression that promotes the activation of the myogenic program, and is therefore termed Linc-RAM (Linc-RNA Activator of Myogenesis). Linc-RAM is transcribed from an intergenic region of myogenic cells and its expression is upregulated during myogenesis. Notably, in vivo functional studies show that Linc-RAM knockout mice display impaired muscle regeneration due to the differentiation defect of satellite cells. Mechanistically, Linc-RAM regulates expression of myogenic genes by directly binding MyoD, which in turn promotes the assembly of the MyoD-Baf60c-Brg1 complex on the regulatory elements of target genes. Collectively, our findings reveal the functional role and molecular mechanism of a lineage-specific Linc-RAM as a regulatory lncRNA required for tissues-specific chromatin remodelling and gene expression.
authors.affiliation: The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Department of Biochemistry and Molecular Biology, School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Yu
authors.affiliation: The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Department of Biochemistry and Molecular Biology, School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Biomedical Informatics, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Ma
authors.affiliation: The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Department of Biochemistry and Molecular Biology, School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
authors.forename: Haixue
authors.initials: H
authors.lastname: Jia
authors.affiliation: The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Department of Biochemistry and Molecular Biology, School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.affiliation: The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Department of Biochemistry and Molecular Biology, School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
authors.forename: Yixia
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Department of Biochemistry and Molecular Biology, School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhai
authors.affiliation: The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Department of Biochemistry and Molecular Biology, School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Zhong
authors.affiliation: The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Department of Biochemistry and Molecular Biology, School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
authors.forename: Changyin
authors.initials: C
authors.lastname: Li
authors.affiliation: The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Department of Biochemistry and Molecular Biology, School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Zou
authors.affiliation: The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Department of Biochemistry and Molecular Biology, School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Meng
authors.affiliation: Department of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China.
authors.forename: Antony K
authors.initials: AK
authors.lastname: Chen
authors.affiliation: Developmental Aging and Regeneration Program, Sanford-Burnham-Prebys Medical Discovery Institute, La Jolla, California 92037, USA.|Department of Epigenetics and Regenerative Medicine, IRCCS Fondazione Santa Lucia, Rome 00161, Italy.
authors.forename: Pier Lorenzo
authors.initials: PL
authors.lastname: Puri
authors.affiliation: The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Department of Biochemistry and Molecular Biology, School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
authors.forename: Meihong
authors.initials: M
authors.lastname: Chen
authors.affiliation: The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Department of Biochemistry and Molecular Biology, School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
authors.forename: Dahai
authors.initials: D
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D024510:Muscle Development; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism; D018482:Muscle, Skeletal / Q000254:growth & development / Q000378:metabolism; D017570:MyoD Protein / Q000235:genetics / Q000378:metabolism*; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D009124:Muscle Proteins; D017570:MyoD Protein; D009687:Nuclear Proteins; D062085:RNA, Long Noncoding; C494055:Smarcd3 protein, mouse; D014157:Transcription Factors; C513194:Smarca4 protein, mouse; D004265:DNA Helicases