title: Ilex paraguariensis supplementation may be an effective nutritional approach to modulate oxidative stress during perimenopause.
abstract: Perimenopause is a period in a woman's life that precedes menopause and is characterized by hormonal changes that result in increased oxidative stress. Since oxidative stress is associated with age-related diseases and perimenopausal symptoms including somato-vegetative manifestations, nutritional antioxidant supplementation may be an effective approach to minimizing this stress. Mate tea (MT) (Ilex paraguariensis), a typical and inexpensive beverage consumed in the Brazilian south-east, Argentina and Uruguay, increases antioxidant defense. We hypothesized that MT could minimize oxidative stress during perimenopause by modulating enzymatic antioxidant defense. To test this, we analyzed the lipid oxidative damage and antioxidant defense in erythrocytes and liver of rats, after MT treatment. Female Wistar rats (aged 16months) in proven perimenopause period received 20mg/kgBW/day of mate tea, by gavage (PM+MT group) or water (PM group). Female rats aged 4months (AD group) received water. Erythrocytes and liver were used to determine lipid oxidative damage, determined by malondialdehyde (MDA); superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) activities. Total plasma antioxidant capacity was examined by ferric reducing antioxidant power assay (FRAP) and estrogen by radioimmunoassay. MT increased FRAP and did not change estrogen levels. Increased SOD and GPx, and reduced MDA were observed in both tissues studied. Increased CAT activity was observed only in the liver. We confirmed the hypothesis that MT was capable of minimizing oxidative stress in this period of life by modulating antioxidant defense.
authors.affiliation: Programa Multicêntrico de Pós-Graduação em Ciências Fisiológicas, SBFis/UNESP, Univ Estadual Paulista, Araçatuba, São Paulo, Brazil; Laboratory of Biochemistry, Department of Basic Sciences, Araçatuba Dental School, UNESP - Univ Estadual Paulista, Araçatuba, São Paulo, Brazil.
authors.forename: Ariana Aparecida Ferreira
authors.initials: AAF
authors.lastname: Pereira
authors.affiliation: Programa Multicêntrico de Pós-Graduação em Ciências Fisiológicas, SBFis/UNESP, Univ Estadual Paulista, Araçatuba, São Paulo, Brazil; Laboratory of Biochemistry, Department of Basic Sciences, Araçatuba Dental School, UNESP - Univ Estadual Paulista, Araçatuba, São Paulo, Brazil.
authors.forename: Keny Gonçalves
authors.initials: KG
authors.lastname: Tirapeli
authors.affiliation: Laboratory of Biochemistry, Department of Basic Sciences, Araçatuba Dental School, UNESP - Univ Estadual Paulista, Araçatuba, São Paulo, Brazil.
authors.forename: Antonio Hernandes
authors.initials: AH
authors.lastname: Chaves-Neto
authors.affiliation: Laboratory of Biochemistry, Department of Basic Sciences, Araçatuba Dental School, UNESP - Univ Estadual Paulista, Araçatuba, São Paulo, Brazil.
authors.forename: Matheus
authors.initials: M
authors.lastname: da Silva Brasilino
authors.affiliation: Department of Organic Chemistry, Chemistry Institute, UNESP - Univ Estadual Paulista, Araraquara, São Paulo, Brazil.
authors.forename: Cláudia Quintino
authors.initials: CQ
authors.lastname: da Rocha
authors.affiliation: Department of Physiology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
authors.forename: Adriane
authors.initials: A
authors.lastname: Belló-Klein
authors.affiliation: Department of Analytical Chemistry and Physical Chemistry, School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina.
authors.forename: Suzana Francisca
authors.initials: SF
authors.lastname: Llesuy
authors.affiliation: Programa Multicêntrico de Pós-Graduação em Ciências Fisiológicas, SBFis/UNESP, Univ Estadual Paulista, Araçatuba, São Paulo, Brazil.
authors.forename: Rita Cássia Menegati
authors.initials: RCM
authors.lastname: Dornelles
authors.affiliation: Programa Multicêntrico de Pós-Graduação em Ciências Fisiológicas, SBFis/UNESP, Univ Estadual Paulista, Araçatuba, São Paulo, Brazil; Laboratory of Biochemistry, Department of Basic Sciences, Araçatuba Dental School, UNESP - Univ Estadual Paulista, Araçatuba, São Paulo, Brazil. Electronic address: anacmsn@foa.unesp.br.
authors.forename: Ana Cláudia de Melo Stevanato
authors.initials: ACMS
authors.lastname: Nakamune
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D019587:Dietary Supplements; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D030019:Ilex paraguariensis; D008099:Liver / Q000378:metabolism; D008315:Malondialdehyde / Q000097:blood; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D047648:Perimenopause; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Antioxidant capacity; Mate; Oxidative damage; Oxidative stress; Perimenopause