title: Effects of Supernatants from Escherichia coli Nissle 1917 and Faecalibacterium prausnitzii on Intestinal Epithelial Cells and a Rat Model of 5-Fluorouracil-Induced Mucositis.
abstract: Faecalibacterium prausnitzii (Fp) and Escherichia coli Nissle 1917 (EcN) are probiotics, which have been reported to ameliorate certain gastrointestinal disorders. We evaluated the effects of supernatants (SN) derived from Fp and EcN on 5-fluorouracil (5-FU)-treated intestinal cells and in a rat model of mucositis. In vitro: IEC-6, Caco-2, and T-84 cells were analyzed for viability and monolayer permeability. In vivo: Female dark agouti rats were gavaged with Fp or EcN SN and injected intraperitoneally with saline (control) or 5-FU to induce mucositis. Rats were euthanized and intestinal tissues collected for myeloperoxidase assay and histological analyses. In vitro: Caco-2 cell viability was further reduced when treated with Fp SN + 5-FU compared to 5-FU controls. In both Caco-2 and T-84 cells, Fp SN partially prevented the decrease in transepithelial electrical resistance (TER) caused by 5-FU administration. In vivo: 5-FU-injected rats administered Fp SN or EcN SN partly prevented body weight loss and normalized water intake compared to 5-FU controls. These results suggest a growth inhibitory mechanism of Fp SN action on transformed epithelial cells that could be mediated by effects on tight junctions. Factors derived from Fp SN and EcN SN could have a role in reducing the severity of intestinal mucositis.
authors.affiliation: a School of Animal and Veterinary Sciences , University of Adelaide , Adelaide , South Australia , Australia.
authors.forename: Hanru
authors.initials: H
authors.lastname: Wang
authors.affiliation: a School of Animal and Veterinary Sciences , University of Adelaide , Adelaide , South Australia , Australia.
authors.forename: Yoga D
authors.initials: YD
authors.lastname: Jatmiko
authors.affiliation: b School of Agriculture , Food and Wine, University of Adelaide , Adelaide , South Australia , Australia.
authors.forename: Susan E P
authors.initials: SE
authors.lastname: Bastian
authors.affiliation: c Gastroenterology Department , Women's and Children's Hospital , Adelaide , South Australia , Australia.|d School of Medicine and Pharmacology, The University of Western Australia, Harry Perkins Institute for Medical Research, Fiona Stanley Hospital , Murdoch , Western Australia , Australia.
authors.forename: Suzanne
authors.initials: S
authors.lastname: Mashtoub
authors.affiliation: a School of Animal and Veterinary Sciences , University of Adelaide , Adelaide , South Australia , Australia.|c Gastroenterology Department , Women's and Children's Hospital , Adelaide , South Australia , Australia.
authors.forename: Gordon S
authors.initials: GS
authors.lastname: Howarth
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D018938:Caco-2 Cells; D002470:Cell Survival; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects; D004926:Escherichia coli; D000070037:Faecalibacterium prausnitzii; D005260:Female; D005472:Fluorouracil / Q000009:adverse effects*; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology; D007422:Intestines / Q000187:drug effects / Q000473:pathology; D052016:Mucositis / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*; D019936:Probiotics / Q000494:pharmacology*; D051381:Rats
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D017077:Culture Media, Conditioned; D007155:Immunologic Factors; D005472:Fluorouracil