title: Role of IL-13 Genetic Variants in Signalling of Asthma.
abstract: Asthma is a chronic inflammatory disease of the lower airways characterised by intermittent airway narrowing and airflow obstruction. The aim of this study was to examine the association of IL-13 Arg 130 Gln (A/G) and -1112C/T cytokine gene polymorphisms and to know the secretion of IL-13 cytokine levels and the interactions between the IL-13 130A/G and IL-13Rα1/IL-4Rα complex cytokine genes. The study population comprised of atopic and non-atopic asthma patients and healthy controls (HC) (N = 120). Single nucleotide polymorphisms (SNPs) were determined by restriction fragment length polymorphism (RFLP). IL-13 cytokine serum levels were measured by enzyme-linked immunosorbent assay (ELISA), and homology modelling of IL-13 A/G cytokine gene was performed through in silico analysis. In IL-13 130A/G cytokine gene AG, GG genotypes (p < 0.0042, OR = 2.87, CI 1.46-5.65; OR = 1.92, CI 1.06-3.48) were found to be significant in atopic asthma patients vs HC. The mean IL-13 serum cytokine levels were found to be significantly high in atopic (38.48 ± 36.54) and non-atopic (36.05 ± 34.54) asthma patients whereas total serum IgE levels were significantly high at p < 0.0001 in atopic and low in non-atopic asthma patients at p < 0.003 compared to HC. In silico analysis indicated that residue IL-13 130 with charge modifying variants was crucial in ligand-receptor interactions. IL-13 cytokine serum levels were significantly high in atopic and non-atopic asthma patients compared to HC. The GG genotype of IL-13 130A/G cytokine gene might be involved in the induced production of total IgE and IL-13 cytokine serum levels suggesting IL-13 may be important in the signalling of asthma.
authors.affiliation: Bhagwan Mahavir Medical Research Centre, Hyderabad, Telangana, 500004, India.
authors.forename: Madhavi Latha
authors.initials: ML
authors.lastname: Alasandagutti
authors.affiliation: Bhagwan Mahavir Medical Research Centre, Hyderabad, Telangana, 500004, India.
authors.forename: Mohd Soheb Sadat
authors.initials: MS
authors.lastname: Ansari
authors.affiliation: Osmania University, Hyderabad, Telangana, 500007, India.
authors.forename: S R
authors.initials: SR
authors.lastname: Sagurthi
authors.affiliation: LEPRA India-Blue Peter Public Health & Research Centre, Cherlapally, Hyderabad, Telangana, 501301, India.
authors.forename: Vijayalakshmi
authors.initials: V
authors.lastname: Valluri
authors.affiliation: Bhagwan Mahavir Medical Research Centre, Hyderabad, Telangana, 500004, India. sumanlathag@yahoo.com.|Osmania University, Hyderabad, Telangana, 500007, India. sumanlathag@yahoo.com.
authors.forename: Sumanlatha
authors.initials: S
authors.lastname: Gaddam
mesh_terms: D000328:Adult; D001249:Asthma / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D005838:Genotype; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000097:blood / Q000235:genetics; D007073:Immunoglobulin E / Q000097:blood; D018793:Interleukin-13 / Q000097:blood / Q000235:genetics* / Q000502:physiology; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D015398:Signal Transduction
chemical_list: D018793:Interleukin-13; D007073:Immunoglobulin E
keywords: association studies; cytokine serum levels; homology modelling; signalling in asthma; total serum IgE