title: Lactobacillus rhamnosus GR-1 Attenuates Induction of Hypertrophy in Cardiomyocytes but Not through Secreted Protein MSP-1 (p75).
abstract: Previous animal studies have shown that the administration of probiotic Lactobacillus rhamnosus can provide a protective effect against ischemia/reperfusion and necrotic injury to the intestine, liver, and heart, as well as a therapeutic effect to the outcome of ischemic injury to the heart, including cardiac hypertrophy and heart failure. We hypothesized that L. rhamnosus GR-1 major secreted protein 1 (MSP-1), also known as p75, plays a major role in this phenomenon. Experiments using neonatal rat ventricular cardiomyocytes showed that live and dead GR-1 bacteria, probiotic-conditioned media, and other probiotic species and strains inhibited the α1-adrenergic receptor agonist phenylephrine-induced hypertrophy as assessed by markers atrial natriuretic peptide and α-skeletal actin. However, using a mutant strain, we showed that this MSP-1 was not required for the inhibition. The ability of factors produced by lactobacilli to improve cardiac function warrants further study for the management of cardiac hypertrophy and heart failure.
authors.affiliation: Lawson Health Research Institute, London, Ontario, Canada.|Department of Microbiology and Immunology, University of Western Ontario, London, Canada.
authors.forename: Grace
authors.initials: G
authors.lastname: Ettinger
authors.affiliation: Lawson Health Research Institute, London, Ontario, Canada.|Department of Microbiology and Immunology, University of Western Ontario, London, Canada.|Department of Surgery (Urology), University of Western Ontario, London, Canada.
authors.forename: Jeremy P
authors.initials: JP
authors.lastname: Burton
authors.affiliation: Lawson Health Research Institute, London, Ontario, Canada.|Department of Biochemistry, University of Western Ontario, London, Canada.
authors.forename: Gregory B
authors.initials: GB
authors.lastname: Gloor
authors.affiliation: Lawson Health Research Institute, London, Ontario, Canada.|Department of Microbiology and Immunology, University of Western Ontario, London, Canada.|Department of Surgery (Urology), University of Western Ontario, London, Canada.
authors.forename: Gregor
authors.initials: G
authors.lastname: Reid
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D009320:Atrial Natriuretic Factor / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D006332:Cardiomegaly / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D004195:Disease Models, Animal; D006333:Heart Failure / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D052201:Lacticaseibacillus rhamnosus / Q000254:growth & development*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010656:Phenylephrine / Q000633:toxicity; D019936:Probiotics / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001426:Bacterial Proteins; D010656:Phenylephrine; D009320:Atrial Natriuretic Factor