title: Reclassification of non-type strain Clostridium pasteurianum NRRL B-598 as Clostridium beijerinckii NRRL B-598.
abstract: The complete genome sequence of non-type strain Clostridium pasteurianum NRRL B-598 was introduced last year; it is an oxygen tolerant, spore-forming, mesophilic heterofermentative bacterium with high hydrogen production and acetone-butanol fermentation ability. The basic genome statistics have shown its similarity to C. beijerinckii rather than the C. pasteurianum species. Here, we present a comparative analysis of the strain with several other complete clostridial genome sequences. Besides a 16S rRNA gene sequence comparison, digital DNA-DNA hybridization (dDDH) and phylogenomic analysis confirmed an inaccuracy of the taxonomic status of strain Clostridium pasteurianum NRRL B-598. Therefore, we suggest its reclassification to be Clostridium beijerinckii NRRL B-598. This is a specific strain and is not identical to other C. beijerinckii strains. This misclassification explains its unexpected behavior, different from other C. pasteurianum strains; it also permits better understanding of the bacterium for a future genetic manipulation that might increase its biofuel production potential.
authors.affiliation: Department of Biomedical Engineering, Brno University of Technology, Technicka 12, 616 00 Brno, Czechia. Electronic address: sedlar@feec.vutbr.cz.
authors.forename: Karel
authors.initials: K
authors.lastname: Sedlar
authors.affiliation: Department of Biotechnology, University of Chemistry and Technology Prague, Technicka 5, 166 28 Prague, Czechia. Electronic address: jan.kolek@vscht.cz.
authors.forename: Jan
authors.initials: J
authors.lastname: Kolek
authors.affiliation: Department of Biomedical Engineering, Brno University of Technology, Technicka 12, 616 00 Brno, Czechia. Electronic address: provaznik@feec.vutbr.cz.
authors.forename: Ivo
authors.initials: I
authors.lastname: Provaznik
authors.affiliation: Department of Biotechnology, University of Chemistry and Technology Prague, Technicka 5, 166 28 Prague, Czechia. Electronic address: petra.patakova@vscht.cz.
authors.forename: Petra
authors.initials: P
authors.lastname: Patakova
mesh_terms: D003013:Clostridium / Q000235:genetics*; D046970:Clostridium beijerinckii / Q000145:classification* / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D016680:Genome, Bacterial; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: Clostridium beijerinckii; Clostridium pasteurianum; Phylogenomic analysis; Reclassification