title: Soluble BAFF Cytokine Levels and Antibody-Mediated Rejection of the Kidney Allograft.
abstract: The B-cell activating factor (BAFF) cytokine has important functions for the survival and maturation of B lymphocytes, which implies that this cytokine might play a role in the development of antibody-mediated rejection (AMR) after kidney transplantation. In our study, we compared the concentrations of the soluble BAFF cytokine in kidney graft recipients with AMR and patients without rejection with the goal of testing the hypothesis whether BAFF level measurement might be useful as a diagnostic marker of AMR. The study included a cohort of 19 high-risk patients with diagnosed AMR and 17 control patients free of rejection. BAFF was measured in all patients before transplantation, during the rejection episodes, and three months after transplantation in patients free of rejection using the Luminex technique. Before transplantation, the serum concentrations of BAFF in patients with AMR and kidney recipients without rejection did not significantly differ. After transplantation, however, BAFF levels were significantly lower in patients with AMR and also in patients with concurrent humoral and cellular rejection compared with patients without rejection (p < 0.05 and p < 0.01, respectively). No correlation was found between BAFF and the production of donor-specific antibodies (DSA) before and after transplantation. Patients experiencing AMR and simultaneous cellular and AMR had significantly lower concentrations of BAFF in comparison with patients free of rejection.
authors.affiliation: Department of Immunogenetics, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21, Prague, Czech Republic. antonij.slavcev@ikem.cz.
authors.forename: Antonij
authors.initials: A
authors.lastname: Slavcev
authors.affiliation: Department of Immunogenetics, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21, Prague, Czech Republic.
authors.forename: Jitka
authors.initials: J
authors.lastname: Brozova
authors.affiliation: Clinic of Nephrology, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21, Prague, Czech Republic.
authors.forename: Janka
authors.initials: J
authors.lastname: Slatinska
authors.affiliation: Department of Immunogenetics, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21, Prague, Czech Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Sekerkova
authors.affiliation: Department of Clinical and Transplantation Pathology, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21, Prague, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Honsova
authors.affiliation: Unit of Medical Statistics, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21, Prague, Czech Republic.
authors.forename: Jelena
authors.initials: J
authors.lastname: Skibova
authors.affiliation: Department of Clinical and Transplantation Immunology, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21, Prague, Czech Republic.
authors.forename: Ilja
authors.initials: I
authors.lastname: Striz
authors.affiliation: Clinic of Nephrology, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21, Prague, Czech Republic.
authors.forename: Ondrej
authors.initials: O
authors.lastname: Viklicky
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D053264:B-Cell Activating Factor / Q000097:blood*; D001402:B-Lymphocytes / Q000276:immunology; D015331:Cohort Studies; D005260:Female; D006084:Graft Rejection / Q000097:blood / Q000276:immunology*; D006680:HLA Antigens / Q000737:chemistry*; D006801:Humans; D007518:Isoantibodies / Q000097:blood*; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D051437:Renal Insufficiency / Q000276:immunology* / Q000601:surgery; D012306:Risk; D013997:Time Factors; D014019:Tissue Donors
chemical_list: D053264:B-Cell Activating Factor; D006680:HLA Antigens; D007518:Isoantibodies; C505719:TNFSF13B protein, human
keywords: Antibody-mediated rejection; BAFF; HLA; Kidney transplantation