title: Ectopic expression of two AREB/ABF orthologs increases drought tolerance in cotton (Gossypium hirsutum).
abstract: Plants have evolved complex molecular, cellular and physiological mechanisms to respond to environmental stressors. Because of the inherent complexity of this response, genetic manipulation to substantially improve water deficit tolerance, particularly in agricultural crops, has been largely unsuccessful, as the improvements are frequently accompanied by slower growth and delayed reproduction. Here, we ectopically express two abiotic stress-responsive bZIP AREB/ABF transcription factor orthologs, Arabidopsis ABF3 and Gossypium hirsutum ABF2D, in G. hirsutum, to compare the effects of exogenous and endogenous AREB/ABF transgene overexpression on dehydration resilience. Our results show that ectopic expression of each of these orthologs increases dehydration resilience, although these increases are accompanied by slower growth. These phenotypic effects are proportional to the ectopic expression level in the GhABF2D transgenic plants, while the phenotypes of all of the AtABF3 transgenic plants are similar, largely independent of ectopic expression level, possibly indicating differential post-transcriptional regulation of these transgenes. Our results indicate that overexpression of exogenous and endogenous ABF homologs in G. hirsutum substantially increases drought resilience, primarily through stomatal regulation, negatively impacting transpiration and photosynthetic productivity.
authors.affiliation: Institute for Agricultural Biosciences, Oklahoma State University, Ardmore, OK, 73401, USA.|Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK, 74078, USA.
authors.forename: Tyson C C
authors.initials: TCC
authors.lastname: Kerr
authors.affiliation: Institute for Agricultural Biosciences, Oklahoma State University, Ardmore, OK, 73401, USA.|Department of Agricultural Botany, Cairo University, Giza, 12613, Egypt.
authors.forename: Haggag
authors.initials: H
authors.lastname: Abdel-Mageed
authors.affiliation: Institute for Agricultural Biosciences, Oklahoma State University, Ardmore, OK, 73401, USA.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Aleman
authors.affiliation: Institute for Agricultural Biosciences, Oklahoma State University, Ardmore, OK, 73401, USA.
authors.forename: Joohyun
authors.initials: J
authors.lastname: Lee
authors.affiliation: USDA-ARS Cropping Systems Research Laboratory, Lubbock, TX, 779415, USA.
authors.forename: Paxton
authors.initials: P
authors.lastname: Payton
authors.affiliation: Institute for Agricultural Biosciences, Oklahoma State University, Ardmore, OK, 73401, USA.
authors.forename: Dakota
authors.initials: D
authors.lastname: Cryer
authors.affiliation: Institute for Agricultural Biosciences, Oklahoma State University, Ardmore, OK, 73401, USA.|Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK, 74078, USA.
authors.forename: Randy D
authors.identifier: 0000-0003-2111-2629
authors.initials: RD
authors.lastname: Allen
mesh_terms: D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism*; D018556:Crops, Agricultural; D055864:Droughts; D000066630:Ectopic Gene Expression; D003368:Gossypium / Q000235:genetics / Q000502:physiology*; D010641:Phenotype; D010788:Photosynthesis; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D054046:Plant Stomata / Q000235:genetics / Q000502:physiology; D018526:Plant Transpiration / Q000502:physiology; D030821:Plants, Genetically Modified; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C506773:ABF3 protein, Arabidopsis; D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; D010940:Plant Proteins; D014157:Transcription Factors
keywords: abiotic stress; abscisic acid; drought; transgenic plants