title: A new paper-based analytical device for detection of Glucose-6-phosphate dehydrogenase deficiency.
abstract: Glucose-6-phosphate dehydrogenase (G6PD) deficiency is a genetic haemolytic disorder. Most persons with G6PD deficiency are asymptomatic, but exposure to oxidant drugs, such as the anti-malarial drug primaquine, may induce haemolysis, which is commonly found in Asian countries. A reliable test is necessary for diagnosing the deficiency to prevent an acute haemolytic crisis. This study proposes a novel quantitative method to detect G6PD deficiency using paper-based analytical devices (G6PDD-PAD). Wax printing was utilized for fabricating circular reaction zone patterns in paper. The colorimetric assay is based on the formation of formazan via a reduction of tetra-nitro blue tetrazolium (TNBT) by the G6PD enzyme on G6PDD-PAD. Detection was achieved by capturing the colour using a desktop scanner and the colour intensity was analysed with Adobe Photoshop C56. The results showed that the G6PD activity analysed by G6PDD-PAD was highly correlated with the standard biochemical assay (SBA) (r2=0.87, p<0.01). Moreover, good agreement by Bland-Altman bias plot was demonstrated between G6PDD-PAD and the SBA (mean bias 1.4 IU/gHb). The detection limit was 0 IU/gHb of G6PD activity. This study demonstrates the feasibility of using G6PDD-PAD. This simple, low-cost test ($0.1/test) should be useful for diagnosing G6PD deficiency in resource-limited settings.
authors.affiliation: Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Patumwan, Bangkok 10330, Thailand.
authors.forename: Phuritat
authors.initials: P
authors.lastname: Kaewarsa
authors.affiliation: Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Patumwan, Bangkok 10330, Thailand.
authors.forename: Wanida
authors.initials: W
authors.lastname: Laiwattanapaisal
authors.affiliation: Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Patumwan, Bangkok 10330, Thailand.
authors.forename: Attakorn
authors.initials: A
authors.lastname: Palasuwan
authors.affiliation: Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Patumwan, Bangkok 10330, Thailand. Electronic address: nantadao@gmail.com.
authors.forename: Duangdao
authors.initials: D
authors.lastname: Palasuwan
mesh_terms: D003124:Colorimetry / Q000295:instrumentation*; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000175:diagnosis*; D006801:Humans; D010209:Paper; D011327:Printing
keywords: G6PD; Glucose-6-phosphate dehydrogenase deficiency; Paper-based analytical devices; Quantitative test; Screening test