title: Molecular breeding of a novel orange-brown tomato fruit with enhanced beta-carotene and chlorophyll accumulation.
abstract: BACKGROUND
  Tomatoes provide a significant dietary source of the carotenoids, lycopene and β-carotene. During ripening, carotenoid accumulation determines the fruit colors while chlorophyll degradation. These traits have been, and continue to be, a significant focus for plant breeding efforts. Previous work has found strong evidence for a relationship betweenCYC-Bgene expression and the orange color of fleshy fruit. Other work has identified a point mutation inSGRthat impedes chlorophyll degradation and causes brown flesh color to be retained in some tomato varieties.
  
  
  METHODS
  We crossed two inbred lines, KNY2 (orange) and KNB1 (brown) and evaluated the relationship between these genes for their effect on fruit color. Phenotypes of F2 generation plants were analyzed and a novel 'orange-brown' fruit color was identified.
  
  
  RESULTS
  We confirm two SNPs, one inCYC-Band another inSGRgene sequence, associated with segregation of 'orange-brown' fruit color in F2 generation. The carotenoid and chlorophyll content of a fleshy fruit was assessed across the different phenotypes and showed a strong correlation with expression pattern of carotenoid biosynthesis genes and SGR function. The orange-brown fruit has high β-carotene and chlorophyll. Our results provide valuable information for breeders to develop tomato fruit of a novel color using molecular markers.
authors.affiliation: Department of Horticulture, Sunchon National University, 255, Jungang-ro, Suncheon, Jeonnam 57922 Republic of Korea.
authors.forename: Ranjith Kumar
authors.initials: RK
authors.lastname: Manoharan
authors.affiliation: Department of Horticulture, Sunchon National University, 255, Jungang-ro, Suncheon, Jeonnam 57922 Republic of Korea.
authors.forename: Hee-Jeong
authors.initials: HJ
authors.lastname: Jung
authors.affiliation: Department of Horticulture, Sunchon National University, 255, Jungang-ro, Suncheon, Jeonnam 57922 Republic of Korea.
authors.forename: Indeok
authors.initials: I
authors.lastname: Hwang
authors.affiliation: Department of Horticulture, Sunchon National University, 255, Jungang-ro, Suncheon, Jeonnam 57922 Republic of Korea.
authors.forename: Namhee
authors.initials: N
authors.lastname: Jeong
authors.affiliation: Department of Fisheries Science, Chonnam National University, 50, Daehak-ro, Yeosu, Jeonnam 59626 Republic of Korea.
authors.forename: Kang Hee
authors.initials: KH
authors.lastname: Kho
authors.affiliation: Department of Agricultural Education, Sunchon National University, 255, Jungang-ro, Suncheon, Jeonnam 57922 Republic of Korea.
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Chung
authors.affiliation: Department of Horticulture, Sunchon National University, 255, Jungang-ro, Suncheon, Jeonnam 57922 Republic of Korea.|Present address: Department of Horticulture, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922 Republic of Korea.
authors.forename: Ill-Sup
authors.initials: IS
authors.lastname: Nou
mesh_terms: D002734:Chlorophyll / Q000737:chemistry*; D003433:Crosses, Genetic; D032621:DNA Shuffling; D005638:Fruit / Q000737:chemistry*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018551:Solanum lycopersicum / Q000235:genetics*; D010641:Phenotype; D000069600:Plant Breeding; D020641:Polymorphism, Single Nucleotide; D019207:beta Carotene / Q000737:chemistry*
chemical_list: D019207:beta Carotene; D002734:Chlorophyll
keywords: CYC-B; Chlorophyll; Orange-brown tomato; SGR; β-carotene