title: Effect of timing of collection of salivary metabolomic biomarkers on oral cancer detection.
abstract: The aim of this study is to evaluate the effect of duration after meals for saliva collections for oral cancer detection using metabolomics. Saliva samples were collected from oral cancer patients (n = 22) and controls (n = 44). Saliva from cancer patients was collected 12 h after dinner, and 1.5 and 3.5 h after breakfast. Control subjects fasted >1.5 h prior to saliva collection. Hydrophilic metabolites were analyzed using capillary electrophoresis mass spectrometry. Levels of 51 metabolites differed significantly in controls vs. oral cancer patients at the 12-h fasting time point (P < 0.05). Fifteen and ten metabolites differed significantly at the 1.5- and 3.5-h time points, respectively. The area of under receiver operating characteristic curve for discriminating oral cancer patients from controls was greatest at the 12-h fasting time point. The collection time after meals affects levels of salivary metabolites for oral cancer screening. The 12-h fasting after dinner time point is optimal. This study contributes to design of saliva collection protocols for metabolomics-based biomarker discovery.
authors.affiliation: Department of Dentistry, Oral and Maxillofacial Plastic and Reconstructive Surgery, Faculty of Medicine, Yamagata University, 2-2-2 Iida-nishi, Yamagata, 990-9585, Japan.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Ishikawa
authors.affiliation: Institute for Advanced Biosciences, Keio University, 246-2 Mizukami, Kakuganji, Tsuruoka, Yamagata, 997-0052, Japan. msugi@sfc.keio.ac.jp.|AMED-CREST, AMED, 1-7-1 Otemachi, Chiyoda-Ku, Tokyo, 100-0004, Japan. msugi@sfc.keio.ac.jp.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sugimoto
authors.affiliation: Department of Dentistry, Oral and Maxillofacial Plastic and Reconstructive Surgery, Faculty of Medicine, Yamagata University, 2-2-2 Iida-nishi, Yamagata, 990-9585, Japan.
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Kitabatake
authors.affiliation: School of Dentistry, University of California, 10833 Le Conte Ave., Los Angeles, CA, 90095-1668, USA.
authors.forename: Micheal
authors.initials: M
authors.lastname: Tu
authors.affiliation: Department of Dentistry, Oral and Maxillofacial Plastic and Reconstructive Surgery, Faculty of Medicine, Yamagata University, 2-2-2 Iida-nishi, Yamagata, 990-9585, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Sugano
authors.affiliation: Department of Dentistry, Oral and Maxillofacial Plastic and Reconstructive Surgery, Faculty of Medicine, Yamagata University, 2-2-2 Iida-nishi, Yamagata, 990-9585, Japan.
authors.forename: Iku
authors.initials: I
authors.lastname: Yamamori
authors.affiliation: Department of Dentistry, Oral and Maxillofacial Plastic and Reconstructive Surgery, Faculty of Medicine, Yamagata University, 2-2-2 Iida-nishi, Yamagata, 990-9585, Japan.
authors.forename: Asuka
authors.initials: A
authors.lastname: Iba
authors.affiliation: Department of Dentistry, Oral and Maxillofacial Plastic and Reconstructive Surgery, Faculty of Medicine, Yamagata University, 2-2-2 Iida-nishi, Yamagata, 990-9585, Japan.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Yusa
authors.affiliation: Institute for Advanced Biosciences, Keio University, 246-2 Mizukami, Kakuganji, Tsuruoka, Yamagata, 997-0052, Japan.
authors.forename: Miku
authors.initials: M
authors.lastname: Kaneko
authors.affiliation: Institute for Advanced Biosciences, Keio University, 246-2 Mizukami, Kakuganji, Tsuruoka, Yamagata, 997-0052, Japan.
authors.forename: Sana
authors.initials: S
authors.lastname: Ota
authors.affiliation: Institute for Advanced Biosciences, Keio University, 246-2 Mizukami, Kakuganji, Tsuruoka, Yamagata, 997-0052, Japan.
authors.forename: Kana
authors.initials: K
authors.lastname: Hiwatari
authors.affiliation: Institute for Advanced Biosciences, Keio University, 246-2 Mizukami, Kakuganji, Tsuruoka, Yamagata, 997-0052, Japan.
authors.forename: Ayame
authors.initials: A
authors.lastname: Enomoto
authors.affiliation: Institute for Advanced Biosciences, Keio University, 246-2 Mizukami, Kakuganji, Tsuruoka, Yamagata, 997-0052, Japan.
authors.forename: Tomita
authors.initials: T
authors.lastname: Masaru
authors.affiliation: Department of Dentistry, Oral and Maxillofacial Plastic and Reconstructive Surgery, Faculty of Medicine, Yamagata University, 2-2-2 Iida-nishi, Yamagata, 990-9585, Japan.
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Iino
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D062407:Meals; D055432:Metabolomics; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D012463:Saliva / Q000378:metabolism*; D013048:Specimen Handling; D013997:Time Factors; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor
keywords: Collection condition; Metabolomics; Oral cancer; Saliva