title: A sensitive chemiluminescent immunoassay to detect Chromotrope FB (Chr FB) in foods.
abstract: Chromotrope FB (Chr FB) is a synthetic azo dye permitted for use in foods and medicines. An acceptable daily intake (ADI) of Chr FB was 0-0.5mg/kg in China. In this study, we synthesized a Chr FB hapten with an amino group to prepare its artificial immunogen. Polyclonal antibodies obtained from New Zealand rabbits were applied to develop an indirect competitive chemiluminescent immunoassay (icCLIA) to detect Chr FB in foods. A horseradish peroxidase (HRP)-luminol-H2O2system was used to yield CL signal with p-iodophenol as an enhancement reagent. The method showed good specificity towards Chr FB and could detect as low as 0.02ngmL-1Chr FB in buffer, 0.07ngg-1in yoghurt candy, 0.07ngg-1in vitamin drink and 0.13ngg-1in bread. Compared with HPLC method, the proposed method is more sensitive by two orders of magnitude. The accuracy and precision of this method are acceptable and comparable with HPLC method. Therefore, the proposed method could be used for rapid screening of Chr FB in the mentioned foodstuffs.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Haihe Education Park, 38 Tongyan Road, Tianjin 300353, People's Republic of China.
authors.forename: Kun
authors.initials: K
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Haihe Education Park, 38 Tongyan Road, Tianjin 300353, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Long
authors.affiliation: Department of Pathology, The Central Hospital of Cangzhou, No 16 Xinhua West Road, Cangzhou, Hebei 061001, People's Republic of China.
authors.forename: Rongge
authors.initials: R
authors.lastname: Xing
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Haihe Education Park, 38 Tongyan Road, Tianjin 300353, People's Republic of China.
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow 119991, Russia; A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, Russia.
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Eremin
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Haihe Education Park, 38 Tongyan Road, Tianjin 300353, People's Republic of China. Electronic address: mengmeng@nankai.edu.cn.
authors.forename: Meng
authors.initials: M
authors.lastname: Meng
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Haihe Education Park, 38 Tongyan Road, Tianjin 300353, People's Republic of China. Electronic address: xirimo@nankai.edu.cn.
authors.forename: Rimo
authors.initials: R
authors.lastname: Xi
mesh_terms: D001391:Azo Compounds / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D006241:Haptens / Q000032:analysis / Q000276:immunology; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods*
chemical_list: D001391:Azo Compounds; D006241:Haptens
keywords: Chemiluminescent immunoassay; Chromotrope FB; Food safety; Polyclonal antibodies