title: Association of ATP-Binding Cassette Transporter A1 (ABCA1)-565 C/T Gene Polymorphism with Hypoalphalipoproteinemia and Serum Lipids, IL-6 and CRP Levels.
abstract: BACKGROUND
  ATP-binding cassette transporter A1 (ABCA1) is a membrane integral protein which plays a vital role in High Density Lipoprotein (HDL) metabolism and exerts a protective effect against Hypoalphalipoproteinemia (HA) by mediation of rate-limiting step in HDL biogenesis. In addition, this protein possesses anti-inflammatory effects by inhibiting the production of some inflammatory cytokines in macrophages. This study investigated the association ofABCA1-565 C/T gene polymorphism with HA and serum lipids, IL-6 and CRP levels.
  
  
  METHODS
  A population which consisted of 101 HA and 95 normal subjects were genotyped forABCA1-565C/T polymorphism by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). The serum concentrations of lipids, IL-6 and high sensitive-CRP (hs-CRP) were measured by the relevant methods.
  
  
  RESULTS
  The frequency of T allele was significantly higher in the HA group than the controls (31.7vs. 19.5%, p=0.002). Thus, carriers of the T allele (CT and TT genotypes) had a higher risk for HA (p=0.016, OR=2.04, 95% CI=1.14-3.63). T allele carriers demonstrated decreased HDL-C and increased triglyceride, IL-6 and CRP levels than those with the CC genotype.
  
  
  CONCLUSIONS
  This study suggests that the-565 C/T polymorphism ofABCA1gene is associated with an increased risk of HA, decreased HDL-C and increased TG, IL-6 and CRP.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Mohammad Mahdi
authors.initials: MM
authors.lastname: Babashamsi
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Sohrab
authors.initials: S
authors.lastname: Halalkhor
authors.affiliation: Department of Molecular and Cell biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Moradi Firouzjah
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Hadi
authors.initials: H
authors.lastname: Parsian
authors.affiliation: Department of Cardiology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Seyed Farzad
authors.initials: SF
authors.lastname: Jalali
authors.affiliation: Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Babashamsi
keywords: -565 C/T polymorphism; ABCA1; CRP; Hypoalphalipoproteinemia; IL-6; Lipids