title: Usefulness of FTIR spectroscopy to distinguish rough and smooth variants of Lactobacillus farciminis CNCM-I-3699.
abstract: In this study, the potential of Fourier transform infrared (FTIR) spectroscopy for assessing putative biochemical and structural differences between the two variants, rough (R) and smooth (S), of Lactobacillus farciminis CNCM-I-3699, a pleomorphic strain, was investigated. The main differences observed were localized in the polysaccharide (1200-900 cm-1) and protein (1700-1500 cm-1) regions. Based on spectral information in these two spectral ranges, clustering resulted in a dendrogram that showed a clear discrimination between both morphotypes. Significant increases in favor of morphotype S compared to R at specific wavenumbers for polysaccharides (22.18% vs. 5.24% at 1068 cm-1) and capsular polysaccharides (16% vs. 13.17% at 1048 cm-1) were recorded. Compared to S, the morphotype R exhibits a 1.27-fold higher signal at the wavenumber of 1637 cm-1 assigned to the amide I β-sheet and a 2.71-fold higher signal at the wavenumber of 1513 cm-1 assigned to the tyrosine involved in the β-sheet arrangement of proteins. The FTIR analysis is efficient to separate and give data on mainly surface component differences observed previously between S colony morphotype (ropy and exopolysaccharide positive) and the R colony morphotype (non-ropy but highly autoaggregative).
authors.affiliation: Normandie Univ, UNICAEN, ABTE, 14000 Caen, France.
authors.forename: R
authors.initials: R
authors.lastname: Tareb
authors.affiliation: Normandie Univ, UNICAEN, ABTE, 14000 Caen, France.|Danisco Animal Nutrition - DuPont Industrial Biosciences, Marlborough SN8 1XN, UK.
authors.forename: M
authors.initials: M
authors.lastname: Bernardeau
authors.affiliation: Normandie Univ, UNICAEN, ABTE, 14000 Caen, France.
authors.forename: C
authors.initials: C
authors.lastname: Amiel
authors.affiliation: Normandie Univ, UNICAEN, ABTE, 14000 Caen, France.
authors.forename: J P
authors.initials: JP
authors.lastname: Vernoux
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D007778:Lactobacillus / Q000737:chemistry* / Q000166:cytology; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001426:Bacterial Proteins; D011135:Polysaccharides, Bacterial
keywords: FTIR spectroscopy; Lactobacillus; pleomorphism; polysaccharide; protein