title: A novel acidic pH fluorescent probe based on a benzothiazole derivative.
abstract: A novel acidic pH fluorescent probe 1 based on a benzothiazole derivative has been designed, synthesized and developed. The linear response range covers the acidic pH range from 3.44 to 6.46, which is valuable for pH researches in acidic environment. The evaluated pKa value of the probe 1 is 4.23. The fluorescence enhancement of the studied probe 1 with an increase in hydrogen ions concentration is based on the hindering of enhanced photo-induced electron transfer (PET) process. Moreover, the pH sensor possesses a highly selective response to H+in the presence of metal ions, anions and other bioactive small molecules which would be interfere with its fluorescent pH response. Furthermore, the probe 1 responds to acidic pH with short response time that was less than 1min. The probe 1 has been successfully applied to confocal fluorescence imaging in live HeLa cells and can selectively stain lysosomes. All of such good properties prove it can be used to monitoring pH fluctuations in acidic environment with high sensitivity, pH dependence and short response time.
authors.affiliation: School of Pharmacology, Henan University of Chinese Medicine, Zhengzhou 450046, PR China. Electronic address: maqiujuan104@126.com.
authors.forename: Qiujuan
authors.initials: Q
authors.lastname: Ma
authors.affiliation: School of Pharmacology, Henan University of Chinese Medicine, Zhengzhou 450046, PR China.
authors.forename: Xian
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Pharmacology, Henan University of Chinese Medicine, Zhengzhou 450046, PR China; Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment, Chinese Medicine Development of Henan Province, Zhengzhou 450046, PR China. Electronic address: fengsx221@163.com.
authors.forename: Suxiang
authors.initials: S
authors.lastname: Feng
authors.affiliation: School of Pharmacology, Henan University of Chinese Medicine, Zhengzhou 450046, PR China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Liang
authors.affiliation: Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment, Chinese Medicine Development of Henan Province, Zhengzhou 450046, PR China.
authors.forename: Tiqiang
authors.initials: T
authors.lastname: Zhou
authors.affiliation: School of Pharmacology, Henan University of Chinese Medicine, Zhengzhou 450046, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.affiliation: School of Pharmacology, Henan University of Chinese Medicine, Zhengzhou 450046, PR China.
authors.forename: Zhuoyi
authors.initials: Z
authors.lastname: Ma
mesh_terms: D052160:Benzothiazoles / Q000138:chemical synthesis / Q000737:chemistry*; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D016923:Cell Death; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D066244:Proton Magnetic Resonance Spectroscopy; D013050:Spectrometry, Fluorescence; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet
chemical_list: D052160:Benzothiazoles; D005456:Fluorescent Dyes; C005465:benzothiazole
keywords: Acidic pH fluorescent probe; Benzothiazole derivative; Cell imaging; Enhanced PET process