title: Effects of Lactobacillus plantarum Strain OLL2712 Culture Conditions on the Anti-inflammatory Activities for Murine Immune Cells and Obese and Type 2 Diabetic Mice.
abstract: Studies on the health-promoting effects of lactic acid bacteria (LAB) are numerous, but few provide examples of the relationship between LAB function and culture conditions. We verified the effect of differences in culture conditions onLactobacillus plantarumOLL2712 functionality; this strain exhibits anti-inflammatory activity and preventive effects against metabolic disorders. We measured interleukin-10 (IL-10) and IL-12 production in murine immune cells treated with OLL2712 cells prepared under various culture conditions. The results showed that the IL-10-inducing activities of OLL2712 cells on murine immune cells differed dramatically between OLL2712 groups at different culture phases and using different culture medium components, temperatures, and neutralizing pHs. In particular, exponential-phase cells had much more IL-10-inducing activity than stationary-phase cells. We confirmed that the Toll-like receptor 2 (TLR2) stimulation activity of OLL2712 cells depended on culture conditions in conjunction with IL-10-inducing activity. We also demonstrated functional differences by culture phasesin vivo; OLL2712 cells at exponential phase had more anti-inflammatory activity and anti-metabolic-disorder effects on obese and diabetic mice than those by their stationary-phase counterparts. These results suggest that culture conditions affect the functionality of anti-inflammatory LAB.IMPORTANCEWhile previous studies demonstrated that culture conditions affected the immunomodulatory properties of lactic acid bacteria (LAB), few have comprehensively investigated the relationship between culture conditions and LAB functionality. In this study, we demonstrated several culture conditions ofLactobacillus plantarumOLL2712 for higher anti-inflammatory activity. We also showed that culture conditions concretely influenced the health-promoting functions of OLL2712in vivo, particularly against metabolic disorders. Further, we characterized a novel mechanism by which changing LAB culture conditions affected immunomodulatory properties. Our results suggest that culture condition optimization is important for the production of LAB with anti-inflammatory activity.
authors.affiliation: Lactic Acid Bacteria Research Department, Food Science Research Laboratories, Division of Research and Development, Meiji Co., Ltd., Odawara, Kanagawa, Japan takayuki.toshimitsu@meiji.com.
authors.forename: T
authors.initials: T
authors.lastname: Toshimitsu
authors.affiliation: Lactic Acid Bacteria Research Department, Food Science Research Laboratories, Division of Research and Development, Meiji Co., Ltd., Odawara, Kanagawa, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ozaki
authors.affiliation: Lactic Acid Bacteria Research Department, Food Science Research Laboratories, Division of Research and Development, Meiji Co., Ltd., Odawara, Kanagawa, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Mochizuki
authors.affiliation: Lactic Acid Bacteria Research Department, Food Science Research Laboratories, Division of Research and Development, Meiji Co., Ltd., Odawara, Kanagawa, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Furuichi
authors.affiliation: Lactic Acid Bacteria Research Department, Food Science Research Laboratories, Division of Research and Development, Meiji Co., Ltd., Odawara, Kanagawa, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Asami
mesh_terms: D000818:Animals; D003470:Culture Media / Q000737:chemistry; D003713:Dendritic Cells / Q000276:immunology; D003924:Diabetes Mellitus, Type 2 / Q000276:immunology* / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D056747:Immunomodulation; D016753:Interleukin-10 / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology; D018664:Interleukin-12 / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology; D048191:Lactiplantibacillus plantarum / Q000254:growth & development / Q000502:physiology*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D009765:Obesity / Q000276:immunology* / Q000382:microbiology*; D019936:Probiotics / Q000627:therapeutic use; D013696:Temperature; D051195:Toll-Like Receptor 2 / Q000096:biosynthesis
chemical_list: D003470:Culture Media; C556062:IL10 protein, mouse; C493486:Tlr2 protein, mouse; D051195:Toll-Like Receptor 2; D016753:Interleukin-10; D018664:Interleukin-12
keywords: Lactobacillus plantarum; Toll-like receptor; culture phase; dendritic cell; interleukin-10; interleukin-12; macrophage; metabolic disorder; mono-oleic acid ester; proinflammatory cytokine