title: Characterization of Caveola-Vesicle Complexes (CVCs) Protein, PHIST/CVC-8195inPlasmodium vivax.
abstract: Plasmodium vivaxproduces numerous caveola-vesicle complex (CVC) structures beneath the membrane of infected erythrocytes. Recently, a member helical interspersed subtelomeric (PHIST) superfamily protein, PcyPHIST/CVC-8195, was identified as CVCs-associated protein inPlasmodium cynomolgiand essential for survival of this parasite. Very little information has been documented to date about PHIST/CVC-8195protein inP. vivax. In this study, the recombinant PvPHIST/CVC-8195N and C termini were expressed, and immunoreactivity was assessed using confirmed vivax malaria patients sera by protein microarray. The subcellular localization of PvPHIST/CVC-8195N and C termini in blood stage parasites was also determined. The antigenicity of recombinant PvPHIST/CVC-8195N and C terminal proteins were analyzed by using serum samples from the Republic of Korea. The results showed that immunoreactivities to these proteins had 61% and 43% sensitivity and 96.9% and 93.8% specificity, respectively. The N terminal of PvPHIST/CVC-8195which contains transmembrane domain and export motif (PEXEL; RxLxE/Q/D) produced CVCs location throughout the erythrocytic-stage parasites. However, no fluorescence was detected with antibodies against C terminal fragment of PvPHIST/CVC-8195. These results suggest that the PvPHIST/CVC-8195is localized on the CVCs and may be immunogenic in natural infection ofP. vivax.
authors.affiliation: Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University, Chuncheon, 24341, Korea.|Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230000, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University, Chuncheon, 24341, Korea.|Yangzhou University School of Medicine, Yangzhou 225001, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Lu
authors.affiliation: Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University, Chuncheon, 24341, Korea.
authors.forename: Jin-Hee
authors.initials: JH
authors.lastname: Han
authors.affiliation: Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University, Chuncheon, 24341, Korea.
authors.forename: Seong-Kyun
authors.initials: SK
authors.lastname: Lee
authors.affiliation: Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University, Chuncheon, 24341, Korea.|Laboratory of Pathogen Infection and Immunity, Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu 214122, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University, Chuncheon, 24341, Korea.|Department of Medical Research, Yangon 11191, Myanmar.
authors.forename: Myat Htut
authors.initials: MH
authors.lastname: Nyunt
authors.affiliation: Department of Molecular and Cellular Biochemistry, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Kwon-Soo
authors.initials: KS
authors.lastname: Ha
authors.affiliation: Department of Internal Medicine, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Seok-Ho
authors.initials: SH
authors.lastname: Hong
authors.affiliation: Department of Physiology, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Won Sun
authors.initials: WS
authors.lastname: Park
authors.affiliation: Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University, Chuncheon, 24341, Korea.
authors.forename: Eun-Taek
authors.initials: ET
authors.lastname: Han
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis* / Q000276:immunology; D021941:Caveolae / Q000737:chemistry*; D022162:Cytoplasmic Vesicles / Q000737:chemistry*; D004912:Erythrocytes / Q000737:chemistry* / Q000469:parasitology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010966:Plasmodium vivax / Q000737:chemistry* / Q000276:immunology; D015800:Protozoan Proteins / Q000032:analysis* / Q000276:immunology; D055815:Young Adult
chemical_list: D000953:Antigens, Protozoan; D015800:Protozoan Proteins
keywords: Plasmodium vivax; PvPHIST/CVC-8195; caveola-vesicle complex; immunoreactivity