title: Remote Postconditioning Alone and Combined with Hypothermia Improved Postresuscitation Cardiac and Neurological Outcomes in Swine.
abstract: Objective. Previously, we demonstrated that remote ischemic postconditioning (RIpostC) improved postresuscitation myocardial and cerebral functions in rat. Here, we investigated the effects of RIpostC alone and combined with therapeutic hypothermia (TH) on cardiac and neurological outcomes after CPR in swine.Methods. Twenty-one pigs were subjected to 10 mins of VF and then 5 mins of CPR. The animals were randomized to receive RIpostC alone, or its combination with TH, or sham control. RIpostC was induced by 4 cycles of limb ischemia followed by reperfusion. TH was implemented by surface cooling to reach a temperature of 32-34°C.Results. During 72 hrs after resuscitation, lower level of cardiac troponin I and greater stroke volume and global ejection fraction were observed in animals that received RIpostC when compared to the control. RIpostC also decreased serum levels of neuron-specific enolase and S100B and increased neurologic alertness score after resuscitation. The combination of RIpostC and TH resulted in greater improvement in cardiac and neurological outcomes than RIpostC alone.Conclusion. RIpostC was conducive to improving postresuscitation myocardial and cerebral functions and reducing their organ injuries. Its combination with TH further enhanced its protective effects.
authors.affiliation: Department of Emergency Medicine, Yuyao People's Hospital, Medical School of Ningbo University, Ningbo, China; Institute of Emergency Medicine, Medical School of Ningbo University, Ningbo, China.
authors.forename: Jiefeng
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Emergency Medicine, Yuyao People's Hospital, Medical School of Ningbo University, Ningbo, China; Institute of Emergency Medicine, Medical School of Ningbo University, Ningbo, China.
authors.forename: Zeng
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Department of Emergency Medicine, Yuyao People's Hospital, Medical School of Ningbo University, Ningbo, China; Institute of Emergency Medicine, Medical School of Ningbo University, Ningbo, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Ye
authors.affiliation: Department of Emergency Medicine, Yuyao People's Hospital, Medical School of Ningbo University, Ningbo, China; Institute of Emergency Medicine, Medical School of Ningbo University, Ningbo, China.
authors.forename: Moli
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Emergency Medicine, Yuyao People's Hospital, Medical School of Ningbo University, Ningbo, China; Institute of Emergency Medicine, Medical School of Ningbo University, Ningbo, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Department of Emergency Medicine, Yuyao People's Hospital, Medical School of Ningbo University, Ningbo, China; Institute of Emergency Medicine, Medical School of Ningbo University, Ningbo, China.
authors.forename: Zilong
authors.identifier: 0000-0002-7523-9097
authors.initials: Z
authors.lastname: Li
mesh_terms: D000818:Animals; D016887:Cardiopulmonary Resuscitation / Q000379:methods*; D004195:Disease Models, Animal; D006801:Humans; D007036:Hypothermia, Induced; D019157:Ischemic Preconditioning, Myocardial; D017202:Myocardial Ischemia / Q000097:blood / Q000503:physiopathology / Q000628:therapy*; D009206:Myocardium / Q000473:pathology*; D010751:Phosphopyruvate Hydratase / Q000097:blood; D051381:Rats; D064568:S100 Calcium Binding Protein beta Subunit / Q000097:blood; D013318:Stroke Volume; D013552:Swine; D019210:Troponin I / Q000097:blood
chemical_list: D064568:S100 Calcium Binding Protein beta Subunit; D019210:Troponin I; D010751:Phosphopyruvate Hydratase