title: Identification of Caveolin-1 as an Invasion-Associated Gene in Liver Cancer Cells Using Dendron-Coated DNA Microarrays.
abstract: The conventional gene expression profiling approaches have been replaced with DNA microarrays with exhibiting a powerful high-throughput capacity. Most solid surfaces of DNA microarrays contain such a high area density of functional groups to immobilize capture DNAs to the surface that the hybridization of capture DNAs with cDNA can be hindered, resulting in low intensity and reproducibility. Since our previous works showed that the 9-acid dendron was able to increase the hybridization efficiency, we aimed to demonstrate the feasibility of 9-acid dendron-coated glass slides as an advanced microarray platform for gene expression profiling. The 9-acid dendron-coated DNA microarray could reproducibly obtain the expression levels of 2800 human cancer-associated genes in the two liver cancer lines: Hep3B and SK-Hep1. Among the differentially expressed genes, Caveolin-1 (Cav-1) was identified as the most highly up-regulated gene in invasive SK-Hep1 in comparison to non-motile Hep3B. The overexpression of Cav-1 in Hep3B promoted the cell invasion, whereas its knockdown in SK-Hep1 suppressed the invasive feature, which confirms that the overexpression of Cav-1 is closely associated with cell invasion of liver carcinoma. Collectively, the 9-acid dendron-coated surface could successfully detect the transcript levels of cells, demonstrating its feasible potential to identify the candidate genes for further functional studies or diagnosis of diseases.
authors.affiliation: Department of Biological Sciences, Chonnam National University, Gwangju, South Korea. eungsam.kim@chonnam.ac.kr.|School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, South Korea. eungsam.kim@chonnam.ac.kr.
authors.forename: Eung-Sam
authors.initials: ES
authors.lastname: Kim
authors.affiliation: Department of Life Sciences, Pohang University of Science and Technology, Pohang, South Korea.
authors.forename: Jung Hee
authors.initials: JH
authors.lastname: Kwon
authors.affiliation: Department of Life Sciences, Pohang University of Science and Technology, Pohang, South Korea.
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Shin
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, South Korea.
authors.forename: Sungyong
authors.initials: S
authors.lastname: You
authors.affiliation: Department of Life Sciences, Pohang University of Science and Technology, Pohang, South Korea.|Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang, South Korea.
authors.forename: Sun Mi
authors.initials: SM
authors.lastname: Hong
authors.affiliation: Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang, South Korea. kchoi@postech.ac.kr.
authors.forename: Kwan Yong
authors.initials: KY
authors.lastname: Choi
mesh_terms: D000873:Anthracenes / Q000737:chemistry*; D051242:Caveolin 1 / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D009361:Neoplasm Invasiveness / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015854:Up-Regulation
chemical_list: D000873:Anthracenes; D051242:Caveolin 1; C501244:dendron
keywords: 9-Acid dendron; Caveolin-1; Cell invasion; DNA microarray; Gene expression profiling; Liver cancer