title: Label-Free Separation of Induced Pluripotent Stem Cells with Anti-SSEA-1 Antibody Immobilized Microfluidic Channel.
abstract: When induced pluripotent stem cells (iPSCs) are routinely cultured, the obtained cells are a heterogeneous mixture, including feeder cells and partially differentiated cells. Therefore, a purification process is required to use them in a clinical stage. We described a label-free separation of iPSCs using a microfluidic channel. Antibodies against stage-specific embryonic antigen 1 (SSEA-1) was covalently immobilized on the channel coated with a phospholipid polymer. After injection of the heterogeneous cell suspension containing iPSCs, the velocity of cell movement under a liquid flow condition was measured. The mean velocity of the cell movement was 2.1 mm/sec in the unmodified channel, while that in the channel with the immobilized-antibody was 0.4 mm/sec. The eluted cells were fractionated by eluting time. As a result, the SSEA-1 positive iPSCs were mainly contained in later fractions, and the proportion of iPSCs was increased from 43% to 82% as a comparison with the initial cell suspension. These results indicated that iPSCs were selectively separated by the microfluidic channel. This channel is a promising device for label-free separation of iPSCs based on their pluripotent state.
authors.affiliation: Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute , Suita, Osaka 565-8565, Japan.
authors.forename: Akihisa
authors.identifier: 0000-0002-0594-2422
authors.initials: A
authors.lastname: Otaka
authors.affiliation: Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute , Suita, Osaka 565-8565, Japan.|Department of Materials Chemistry, Ryukoku University , Seta, Otsu 520-2194, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Kitagawa
authors.affiliation: Department of Materials Chemistry, Ryukoku University , Seta, Otsu 520-2194, Japan.
authors.forename: Takahiko
authors.initials: T
authors.lastname: Nakaoki
authors.affiliation: Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute , Suita, Osaka 565-8565, Japan.
authors.forename: Mitsuhi
authors.initials: M
authors.lastname: Hirata
authors.affiliation: Department of Materials Engineering, The University of Tokyo , Bunkyo-ku, Tokyo 113-8656, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Fukazawa
authors.affiliation: Department of Materials Engineering, The University of Tokyo , Bunkyo-ku, Tokyo 113-8656, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ishihara
authors.affiliation: Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute , Suita, Osaka 565-8565, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Mahara
authors.affiliation: Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute , Suita, Osaka 565-8565, Japan.
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Yamaoka
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002469:Cell Separation / Q000379:methods*; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D016256:Lewis X Antigen / Q000276:immunology*; D008689:Methacrylates / Q000138:chemical synthesis / Q000737:chemistry; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D016256:Lewis X Antigen; D008689:Methacrylates; D011109:Polymethacrylic Acids; C513779:poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate-co-p-nitrophenyloxycarbonyl polyethyleneglycol methacrylate); D011092:Polyethylene Glycols