title: Development of a polymerase chain reaction-restriction fragment length polymorphism method for identification of theFusariumgenus using the transcription elongation factor-1α gene.
abstract: Fusariumspecies are well-known plant pathogens and food contaminants that have also appeared as one of the most important groups of medically significant fungi. The sequences of the translation elongation factor (TEF)-1α gene have been broadly employed for species detection. A total of 50 strains ofFusariumspp., including environmental, clinical and reference isolates were used for the current study. The primer sets, Fu3f and Fu3r, were used to amplify an ~420-bp DNA fragment of the TEF-1α gene. Double digestion with two restriction enzymes,XhoI andSduI was used for discrimination of theFusariumspecies in the TEF-1α gene fragment. Double digestion of the TEF-1α gene fragment from five clinically importantFusariumspecies were clearly differentiated from each other: TheF. solanispecies complex,F. oxysporumspecies complex,F. verticillioides,F. proliferatumandF. fujikuroi. This method facilitates detection and enables verification of theFusariumgenus; therefore, it may be applied for disease control.
authors.affiliation: Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 61357-15794, Iran; Department of Medical Mycology, Medical School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 61357-15794, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Zarrin
authors.affiliation: Department of Medical Mycology, Medical School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 61357-15794, Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Ganj
authors.affiliation: Department of Medical Mycology, Medical School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 61357-15794, Iran.
authors.forename: Sama
authors.initials: S
authors.lastname: Faramarzi
keywords: Fusarium; polymerase chain reaction; transcription elongation factor-1α gene