title: Generation of Biostable L-aptamers against Achiral Targets by Chiral Inversion of Existing D-aptamers.
abstract: In this paper, based on reciprocal chiral substrate specificity, taking achiral molecules, ethanolamine (EA) and malachite green (MG) as two model targets, biostable L- DNA aptamers and L-RNA aptamers were generated respectively by chiral inversion of existing D-aptamers. In the detection of EA with L-DNA aptamer-based sensors, the feasibility of our strategy was confirmed, while in the detection of MG with L-RNA aptamers, linear calibration curves were obtained in the range from 0.1 to 5µm with the detection limit of 0.065µm under optimized experimental conditions. The results demonstrated that the mirror-image L-aptamers have identical recognition capability as D-aptamers. Meanwhile, L-aptamers have superior biostability to resist nuclease digestion, protein binding interference and off-target effects, enabling their applications in complex practical samples, such as lake water and fish tissue extractions. Our work provides a simple, yet universal and efficient way to develop biostable aptamers.
authors.affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China.
authors.forename: Huapei
authors.initials: H
authors.lastname: Chen
authors.affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China.
authors.forename: Sitao
authors.initials: S
authors.lastname: Xie
authors.affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Liang
authors.affiliation: Attribute Sciences, Amgen, One Amgen Center Drive, Thousand Oaks, CA 91320, United States.
authors.forename: Cuichen
authors.initials: C
authors.lastname: Wu
authors.affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China. Electronic address: cuiliang@hnu.edu.cn.
authors.forename: Liang
authors.initials: L
authors.lastname: Cui
authors.affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China.
authors.forename: Shuang-Yan
authors.initials: SY
authors.lastname: Huan
authors.affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China. Electronic address: xbzhang@hnu.edu.cn.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Zhang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D004355:Drug Stability; D019856:Ethanolamine / Q000737:chemistry; D012394:Rosaniline Dyes / Q000737:chemistry; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D052157:Aptamers, Nucleotide; D012394:Rosaniline Dyes; C005095:malachite green; D019856:Ethanolamine
keywords: Aptamers; Biostability; Ethanolamine; Malachite green; Mirror image