title: Experimental evaluation of new chitin-chitosan graft for duraplasty.
abstract: Natural materials such as collagen and alginate have promising applications as dural graft substitutes. These materials are able to restore the dural defect and create optimal conditions for the development of connective tissue at the site of injury. A promising material for biomedical applications is chitosan-a linear polysaccharide obtained by the deacetylation of chitin. It has been found to be nontoxic, biodegradable, biofunctional and biocompatible in addition to having antimicrobial characteristics. In this study we designed new chitin-chitosan substitutes for dura mater closure and evaluated their effectiveness and safety. Chitosan films were produced from 3 % of chitosan (molar mass-200, 500 or 700 kDa, deacetylation rate 80-90%) with addition of 20% of chitin. Antimicrobial effictively and cell viability were analysed for the different molar masses of chitosan. The film containing chitosan of molar mass 200 kDa, had the best antimicrobial and biological activity and was successfully used for experimental duraplasty in an in vivo model. In conclusion the chitin-chitosan membrane designed here met the requirements for a dura matter graft exhibiting the ability to support cell growth, inhibit microbial growth and biodegradade at an appropriate rate. Therefore this is a promising material for clinical duroplasty.
authors.affiliation: Medical Institute, Sumy State University, 2, R-Korsakova street, Sumy, 40007, Ukraine. m.pogorielov@gmail.com.
authors.forename: M
authors.identifier: http://orcid.org/0000-0001-9372-7791
authors.initials: M
authors.lastname: Pogorielov
authors.affiliation: Neurosurgery Department, Kharkov National Medical University, Kharkiv, Ukraine.
authors.forename: A
authors.initials: A
authors.lastname: Kravtsova
authors.affiliation: Department of Materials Science and Engineering, INSIGNEO institute for in silico medicine, University of Sheffield, Pam Liversidge Building, Mappin Street, S1 3JD, Sheffield, UK.
authors.forename: G C
authors.initials: GC
authors.lastname: Reilly
authors.affiliation: Medical Institute, Sumy State University, 2, R-Korsakova street, Sumy, 40007, Ukraine.
authors.forename: V
authors.initials: V
authors.lastname: Deineka
authors.affiliation: Department of Materials Science and Engineering, INSIGNEO institute for in silico medicine, University of Sheffield, Pam Liversidge Building, Mappin Street, S1 3JD, Sheffield, UK.
authors.forename: G
authors.initials: G
authors.lastname: Tetteh
authors.affiliation: Institute for Applied Physics, Sumy, Ukraine.
authors.forename: O
authors.initials: O
authors.lastname: Kalinkevich
authors.affiliation: Medical Institute, Sumy State University, 2, R-Korsakova street, Sumy, 40007, Ukraine.
authors.forename: O
authors.initials: O
authors.lastname: Pogorielova
authors.affiliation: Medical Institute, Sumy State University, 2, R-Korsakova street, Sumy, 40007, Ukraine.
authors.forename: R
authors.initials: R
authors.lastname: Moskalenko
authors.affiliation: Medical Institute, Sumy State University, 2, R-Korsakova street, Sumy, 40007, Ukraine.
authors.forename: G
authors.initials: G
authors.lastname: Tkach
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D001861:Bone Regeneration; D002470:Cell Survival; D002482:Cellulose / Q000737:chemistry; D002555:Cerebrospinal Fluid / Q000737:chemistry; D002686:Chitin / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry; D004388:Dura Mater / Q000187:drug effects; D008422:Materials Testing; D008826:Microbial Sensitivity Tests; D011134:Polysaccharides / Q000737:chemistry; D011817:Rabbits; D014945:Wound Healing
chemical_list: D000890:Anti-Infective Agents; D001672:Biocompatible Materials; D011134:Polysaccharides; D002686:Chitin; D002482:Cellulose; D003094:Collagen; D048271:Chitosan