title: The different expression of TRPM7 and MagT1 impacts on the proliferation of colon carcinoma cells sensitive or resistant to doxorubicin.
abstract: The processes leading to anticancer drug resistance are not completely unraveled. To get insights into the underlying mechanisms, we compared colon carcinoma cells sensitive to doxorubicin with their resistant counterpart. We found that resistant cells are growth retarded, and show staminal and ultrastructural features profoundly different from sensitive cells. The resistant phenotype is accompanied by the upregulation of the magnesium transporter MagT1 and the downregulation of the ion channel kinase TRPM7. We demonstrate that the different amounts of TRPM7 and MagT1 account for the different proliferation rate of sensitive and resistant colon carcinoma cells. It remains to be verified whether they are also involved in the control of other "staminal" traits.
authors.affiliation: Dipartimento di Scienze Biomediche e Cliniche L. Sacco, Università di Milano, Via G.B. Grassi 74, Milano I-20157, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Cazzaniga
authors.affiliation: Dipartimento di Scienze Biomediche e Cliniche L. Sacco, Università di Milano, Via G.B. Grassi 74, Milano I-20157, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Moscheni
authors.affiliation: Istituto di Patologia Generale, Facoltà di Medicina, Università Cattolica del Sacro Cuore, Largo F. Vito 1, Roma I-00168, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Trapani
authors.affiliation: Istituto di Patologia Generale, Facoltà di Medicina, Università Cattolica del Sacro Cuore, Largo F. Vito 1, Roma I-00168, Italy.
authors.forename: Federica I
authors.initials: FI
authors.lastname: Wolf
authors.affiliation: Dipartimento di Farmacia e Biotecnologie, Università Alma Mater di Bologna, Via San Donato 19/2, Bologna I-40127, Italy.|Istituto Nazionale Biostrutture e Biosistemi, Viale delle Medaglie d'oro 305, Roma I-00136, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Farruggia
authors.affiliation: Dipartimento di Farmacia e Biotecnologie, Università Alma Mater di Bologna, Via San Donato 19/2, Bologna I-40127, Italy.
authors.forename: Azzurra
authors.initials: A
authors.lastname: Sargenti
authors.affiliation: Dipartimento di Farmacia e Biotecnologie, Università Alma Mater di Bologna, Via San Donato 19/2, Bologna I-40127, Italy.|Istituto Nazionale Biostrutture e Biosistemi, Viale delle Medaglie d'oro 305, Roma I-00136, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Iotti
authors.affiliation: Dipartimento di Scienze Biomediche e Cliniche L. Sacco, Università di Milano, Via G.B. Grassi 74, Milano I-20157, Italy.
authors.forename: Jeanette A M
authors.initials: JA
authors.lastname: Maier
authors.affiliation: Dipartimento di Scienze Biomediche e Cliniche L. Sacco, Università di Milano, Via G.B. Grassi 74, Milano I-20157, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Castiglioni
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D027682:Cation Transport Proteins / Q000235:genetics*; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000235:genetics*; D004317:Doxorubicin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D050053:TRPM Cation Channels / Q000235:genetics*
chemical_list: D000903:Antibiotics, Antineoplastic; D027682:Cation Transport Proteins; C509698:MagT1 protein, human; D050053:TRPM Cation Channels; D004317:Doxorubicin; D017346:Protein Serine-Threonine Kinases; C486583:TRPM7 protein, human