title: Adipose-derived stem cells ameliorate renal interstitial fibrosis through inhibition of EMT and inflammatory response via TGF-β1 signaling pathway.
abstract: Adipose-derived stem cells (ADSCs) have been successfully used to treat acute kidney injury or acute renal failure. However, the effect of ADSCs on treating renal interstitial fibrosis remains unknown. Here, we assessed the therapeutic efficacy of ADSCs on renal interstitial fibrosis induced by unilateral ureter obstruction (UUO) and explored the potential mechanisms. After 7days of UUO, rats were injected with ADSCs (5×106) or vehicle via tail vein. We found that ADSCs administration significantly ameliorated renal interstitial fibrosis, the occurrence of epithelial-mesenchymal transition (EMT) and inflammatory response. Furthermore, ADSCs administration could inhibit the activation of transforming growth factor-β1 (TGF-β1) signaling pathway, which might play a crucial role in renal interstitial fibrosis of the UUO model rats. These results suggested that ADSCs treatment attenuates renal interstitial fibrosis possibly through inhibition of EMT and inflammatory response via TGF-β1 signaling pathway. Therefore, ADSCs may be an effective therapeutic strategy for the treatment of renal interstitial fibrosis.
authors.affiliation: Department of Nephrology, The Second Hospital of Shandong University, Shandong University, Jinan, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Spinal Surgery, The Second Hospital of Shandong University, Shandong University, Jinan, China.
authors.forename: Changliang
authors.initials: C
authors.lastname: Peng
authors.affiliation: Department of Nephrology, The Second Hospital of Shandong University, Shandong University, Jinan, China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Lv
authors.affiliation: Department of Nephrology, The Second Hospital of Shandong University, Shandong University, Jinan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Department of Nephrology, The Second Hospital of Shandong University, Shandong University, Jinan, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Nephrology, The Second Hospital of Shandong University, Shandong University, Jinan, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wen
authors.affiliation: Department of Nephrology, The Second Hospital of Shandong University, Shandong University, Jinan, China. Electronic address: guangj@sdu.edu.cn.
authors.forename: Guangju
authors.initials: G
authors.lastname: Guan
authors.affiliation: Department of Nephrology, The Second Hospital of Shandong University, Shandong University, Jinan, China. Electronic address: lg69007@163.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
mesh_terms: D000273:Adipose Tissue / Q000473:pathology*; D000818:Animals; D002478:Cells, Cultured; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects; D005355:Fibrosis; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000628:therapy*; D007668:Kidney / Q000473:pathology*; D007674:Kidney Diseases / Q000276:immunology / Q000628:therapy*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000502:physiology*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D014517:Ureteral Obstruction / Q000276:immunology / Q000628:therapy*
chemical_list: D053773:Transforming Growth Factor beta1
keywords: ADSCs; EMT; Inflammatory response; Renal interstitial fibrosis; TGF-β1