title: An NPF transporter exports a central monoterpene indole alkaloid intermediate from the vacuole.
abstract: Plants sequester intermediates of metabolic pathways into different cellular compartments, but the mechanisms by which these molecules are transported remain poorly understood. Monoterpene indole alkaloids, a class of specialized metabolites that includes the anticancer agent vincristine, antimalarial quinine and neurotoxin strychnine, are synthesized in several different cellular locations. However, the transporters that control the movement of these biosynthetic intermediates within cellular compartments have not been discovered. Here we present the discovery of a tonoplast localized nitrate/peptide family (NPF) transporter from Catharanthus roseus, CrNPF2.9, that exports strictosidine, the central intermediate of this pathway, into the cytosol from the vacuole. This discovery highlights the role that intracellular localization plays in specialized metabolism, and sets the stage for understanding and controlling the central branch point of this pharmacologically important group of compounds.
authors.affiliation: The John Innes Centre, Department of Biological Chemistry, Norwich Research Park, Norwich NR4 7UK, UK.
authors.forename: Richard M E
authors.initials: RM
authors.lastname: Payne
authors.affiliation: DynaMo Centre, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 40 Thorvaldsensvej, DK-1871 Frederiksberg C, Denmark.|Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 1871 Frederiksberg C, Denmark.
authors.forename: Deyang
authors.initials: D
authors.lastname: Xu
authors.affiliation: Université François-Rabelais de Tours, EA2106 Biomolécules et Biotechnologies Végétales, Département de Biologie et Physiologie Végétales, UFR Sciences et Techniques, Parc de Grandmont 37200 Tours, France.
authors.forename: Emilien
authors.initials: E
authors.lastname: Foureau
authors.affiliation: Université François-Rabelais de Tours, EA2106 Biomolécules et Biotechnologies Végétales, Département de Biologie et Physiologie Végétales, UFR Sciences et Techniques, Parc de Grandmont 37200 Tours, France.
authors.forename: Marta Ines Soares
authors.initials: MI
authors.lastname: Teto Carqueijeiro
authors.affiliation: Université François-Rabelais de Tours, EA2106 Biomolécules et Biotechnologies Végétales, Département de Biologie et Physiologie Végétales, UFR Sciences et Techniques, Parc de Grandmont 37200 Tours, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Oudin
authors.affiliation: Université François-Rabelais de Tours, EA2106 Biomolécules et Biotechnologies Végétales, Département de Biologie et Physiologie Végétales, UFR Sciences et Techniques, Parc de Grandmont 37200 Tours, France.
authors.forename: Thomas Dugé de
authors.identifier: 0000-0002-2205-2886
authors.initials: TD
authors.lastname: Bernonville
authors.affiliation: DynaMo Centre, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 40 Thorvaldsensvej, DK-1871 Frederiksberg C, Denmark.|Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 1871 Frederiksberg C, Denmark.
authors.forename: Vlastimil
authors.identifier: 0000-0001-7890-4593
authors.initials: V
authors.lastname: Novak
authors.affiliation: DynaMo Centre, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 40 Thorvaldsensvej, DK-1871 Frederiksberg C, Denmark.|Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 1871 Frederiksberg C, Denmark.
authors.forename: Meike
authors.identifier: 0000-0002-2350-985X
authors.initials: M
authors.lastname: Burow
authors.affiliation: Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 1871 Frederiksberg C, Denmark.
authors.forename: Carl-Erik
authors.initials: CE
authors.lastname: Olsen
authors.affiliation: The John Innes Centre, Department of Computational and Systems Biology, Norwich Research Park, Norwich NR4 7UK, UK.
authors.forename: D Marc
authors.initials: DM
authors.lastname: Jones
authors.affiliation: The John Innes Centre, Department of Biological Chemistry, Norwich Research Park, Norwich NR4 7UK, UK.
authors.forename: Evangelos C
authors.initials: EC
authors.lastname: Tatsis
authors.affiliation: The John Innes Centre, Department of Cell and Developmental Biology, Norwich Research Park, Norwich NR4 7UK, UK.
authors.forename: Ali
authors.initials: A
authors.lastname: Pendle
authors.affiliation: Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 1871 Frederiksberg C, Denmark.
authors.forename: Barbara
authors.initials: B
authors.lastname: Ann Halkier
authors.affiliation: Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 1871 Frederiksberg C, Denmark.|Section for Plant Biochemistry, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 1871 Frederiksberg C, Denmark.
authors.forename: Fernando
authors.initials: F
authors.lastname: Geu-Flores
authors.affiliation: Université François-Rabelais de Tours, EA2106 Biomolécules et Biotechnologies Végétales, Département de Biologie et Physiologie Végétales, UFR Sciences et Techniques, Parc de Grandmont 37200 Tours, France.
authors.forename: Vincent
authors.identifier: 0000-0001-8902-4532
authors.initials: V
authors.lastname: Courdavault
authors.affiliation: DynaMo Centre, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 40 Thorvaldsensvej, DK-1871 Frederiksberg C, Denmark.|Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 1871 Frederiksberg C, Denmark.
authors.forename: Hussam Hassan
authors.identifier: 0000-0001-6660-0509
authors.initials: HH
authors.lastname: Nour-Eldin
authors.affiliation: The John Innes Centre, Department of Biological Chemistry, Norwich Research Park, Norwich NR4 7UK, UK.
authors.forename: Sarah E
authors.initials: SE
authors.lastname: O'Connor
mesh_terms: D027321:Anion Transport Proteins / Q000235:genetics* / Q000378:metabolism; D001692:Biological Transport; D030008:Catharanthus / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D039821:Monoterpenes / Q000378:metabolism; D000091285:Nitrate Transporters; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D027981:Symporters / Q000235:genetics* / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism; D014748:Vinca Alkaloids / Q000378:metabolism*
chemical_list: D027321:Anion Transport Proteins; D039821:Monoterpenes; D000091285:Nitrate Transporters; D010940:Plant Proteins; D027981:Symporters; D014748:Vinca Alkaloids; C021093:strictosidine