title: Novel assay of antibacterial components in manuka honey using lucigenin-chemiluminescence-HPLC.
abstract: Five components (hydrogen peroxide, methylglyoxal, dihydroxyacetone, fructose and glucose) of New Zealand manuka honey (Leptospermum scoparium) were analyzed using lucigenin chemiluminescence high-performance liquid chromatography (lucigenin-CL-HPLC). We focused on active oxygen species produced from the components in order to easily detect these five components contained in manuka honey. H2O2and O2-generated from these components were identified by lucigenin-CL and electron spin resonance (ESR), and the bactericidal effect of ROS was confirmed using E. coli. The previously reported assays for Manuka honey components have low specificities and require complicated preprocessing methods. As our results, the detection and identification of these components were possible within 30 min in lucigenin-CL-HPLC system, without any special treatment. It is considered that lucigenin-CL-HPLC is useful for the quality control and the analysis of various honey.
authors.affiliation: Department of Analytical Biochemistry, School of Pharmacy, Showa University, 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8555, Japan. Electronic address: koji180@pharm.showa-u.ac.jp.
authors.forename: Koji
authors.initials: K
authors.lastname: Karasawa
authors.affiliation: Department of Analytical Biochemistry, School of Pharmacy, Showa University, 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8555, Japan.
authors.forename: Shiomi
authors.initials: S
authors.lastname: Haraya
authors.affiliation: Department of Analytical Biochemistry, School of Pharmacy, Showa University, 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8555, Japan.
authors.forename: Sachie
authors.initials: S
authors.lastname: Okubo
authors.affiliation: Department of Analytical Biochemistry, School of Pharmacy, Showa University, 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8555, Japan.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Arakawa
mesh_terms: D000166:Acridines; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli; D006722:Honey / Q000032:analysis*; D006861:Hydrogen Peroxide; D049449:Luminescence
chemical_list: D000166:Acridines; D000900:Anti-Bacterial Agents; C033472:10,10'-dimethyl-9,9'-biacridinium; D006861:Hydrogen Peroxide
keywords: Active oxygen; Chemiluminescence; HPLC; Lucigenin; Manuka honey