title: Association between MMP-3 and MMP-9 polymorphisms and coronary artery disease.
abstract: Matrix metalloproteinase (MMP)-3 and MMP-9 polymorphisms are characterized by plaque stability in coronary arteries. The aim of the current study was to investigate the5A/6Apolymorphism in theMMP-3gene and C/T polymorphism in theMMP-9gene in patients with coronary artery disease (CAD). The study population consisted of 400 patients who underwent coronary angiography. There were two groups consisting of 200 consecutive patients with CAD, presenting with stable angina pectoris, and 200 consecutive patients exhibiting normal coronary arteries. Two single nucleotide polymorphisms in theMMPgene,MMP-3andMMP-9, were detected using a polymerase chain reaction-restriction fragment length polymorphism assay. Mean age, gender distribution, smoking status, presence of diabetes mellitus and hypercholesterolemia were identified to be similar between the groups. One hundred and twenty seven (63.5%) patients had hypertension in the CAD group, whereas only 55 (27.5%) patients had hypertension in the control group (P<0.001). No significant difference in frequency of alleles and genotypes ofMMP-9 C→Tbetween the CAD and control groups was identified. The5Aallele frequency ofMMP-3in the CAD group was significantly higher when compared with the control group (P<0.001; odds ratio=2.18). The genotype frequency ofMMP-3 5A/5Ain the CAD group was significantly higher when compared with the controls (P=0.005). When compared with the homozygous wild-type (6A/6A) genotype of theMMP-3gene, the cumulative frequency of heterozygote and homozygote genotypes of theMMP-3gene was significantly higher in the CAD compared with the control group (P<0.001). Thus, the present study demonstrated that the5A/5Aand6A/5A+5A/5Agenotypes of theMMP-3gene were associated with an increased risk of CAD.
authors.affiliation: Department of Cardiology, Heart Center, Cumhuriyet University, Sivas 58140, Turkey.
authors.forename: Osman
authors.initials: O
authors.lastname: Beton
authors.affiliation: Department of Medical Biology, Cumhuriyet University, Sivas 58140, Turkey.
authors.forename: Serdal
authors.initials: S
authors.lastname: Arslan
authors.affiliation: Department of Cardiology, Turkiye Yuksek Ihtisas Research and Training Hospital, Ankara 06100, Turkey.
authors.forename: Burak
authors.initials: B
authors.lastname: Acar
authors.affiliation: Molecular Biology and Genetics, Heart Center, Cumhuriyet University, Sivas 58140, Turkey.
authors.forename: Nil
authors.initials: N
authors.lastname: Ozbilum
authors.affiliation: Cardiovascular Surgery, Heart Center, Cumhuriyet University, Sivas 58140, Turkey.
authors.forename: Ocal
authors.initials: O
authors.lastname: Berkan
keywords: coronary artery disease; gene polymorphism; matrix metalloproteinase-3; matrix metalloproteinase-9