title: Biologic variability of N-terminal pro-brain natriuretic peptide in healthy dogs and dogs with myxomatous mitral valve disease.
abstract: BACKGROUND
  To determine the biologic variability of N-terminal pro-brain natriuretic peptide (NTproBNP) in healthy dogs and dogs with various stages of myxomatous mitral valve disease (MMVD).
  
  
  METHODS
  Thirty-eight privately owned dogs: 28 with MMVD and 10 healthy controls.
  
  
  METHODS
  Prospective clinical study with comprehensive evaluation used to group dogs as healthy or into three stages of MMVD based on current guidelines. NTproBNP was measured hourly, daily, and weekly. For each group, analytical (CVA), within-subject (CVI), and between-subject (CVG) coefficients of variability were calculated in addition to percent critical change value (CCV) and index of individuality (IoI).
  
  
  RESULTS
  For healthy dogs, calculated NTproBNP values were: CVA= 4.2%; CVI= 25.2%; CVG= 49.3%; IoI = 0.52, and CCV = 70.8%. For dogs with MMVD, calculated NTproBNP values were: CVA= 6.2%; CVI= 20.0%; CVG= 61.3%; IoI = 0.34, and CCV = 58.2%.
  
  
  CONCLUSIONS
  Biologic variability affects NTproBNP concentrations in healthy dogs and dogs with MMVD. Monitoring serial individual changes in NTproBNP may be clinically relevant in addition to using population-based reference ranges to determine changes in disease status.
authors.affiliation: Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, USA. Electronic address: rlw0041@auburn.edu.
authors.forename: Randolph L
authors.initials: RL
authors.lastname: Winter
authors.affiliation: Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, USA.
authors.forename: Ashley B
authors.initials: AB
authors.lastname: Saunders
authors.affiliation: Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, USA.
authors.forename: Sonya G
authors.initials: SG
authors.lastname: Gordon
authors.affiliation: Immunoassay R&D, IDEXX Laboratories, Westbrook, ME, USA.
authors.forename: Jesse S
authors.initials: JS
authors.lastname: Buch
authors.affiliation: Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, USA.
authors.forename: Matthew W
authors.initials: MW
authors.lastname: Miller
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood*; D016022:Case-Control Studies; D004283:Dog Diseases / Q000097:blood*; D004285:Dogs / Q000097:blood*; D005260:Female; D008297:Male; D008944:Mitral Valve Insufficiency / Q000097:blood / Q000662:veterinary*; D020097:Natriuretic Peptide, Brain / Q000097:blood*; D010446:Peptide Fragments / Q000097:blood*; D011446:Prospective Studies; D012016:Reference Values
chemical_list: D015415:Biomarkers; D010446:Peptide Fragments; C109794:pro-brain natriuretic peptide (1-76); D020097:Natriuretic Peptide, Brain
keywords: Canine; Cardiac biomarker; Mitral regurgitation; Prospective