title: Level of Granzyme B-positive T-regulatory cells is a strong predictor biomarker of acute Graft-versus-host disease after day +30 after allo-HSCT.
abstract: Acute Graft-versus-host-disease (aGVHD), the major complication and one of the main causes of poor outcomes of allogeneic hematopoietic stem cell transplantation (allo-HSCT). Nowadays there are no widely accepted cell, plasma or another biomarker that can be used for aGVHD prediction. We hypothesized that a level of Granzyme B-positive T regulatory (GZMB-positive Treg) cells on day+30 after allo-HSCT could be the measure of immune response suppression and could predict aGVHD development after day +30. We applied a widespread and easy-to-perform method of multicolor flow cytometry to measure level of GZMB-positive Treg cells. Levels of GZMB-positive Tregs on day +30 after allo-HSCT were significantly higher in those patients who never developed aGVHD in comparison with the other group of patient with aGVHD after day +30 (p=0.0229). We conclude that the level of GZMB-positive Treg cells is a strong predictor of acute Graft-versus-host disease after day +30 after allo-HSCT.
authors.affiliation: Bone Marrow Transplant Department, National Research Center for Hematology, Moscow, Russian Federation; Flow Cytometry Laboratory, National Research Center for Hematology, Moscow, Russian Federation. Electronic address: mdrokov@gmail.com.
authors.forename: Mikhail Y
authors.initials: MY
authors.lastname: Drokov
authors.affiliation: Flow Cytometry Laboratory, National Research Center for Hematology, Moscow, Russian Federation.
authors.forename: Julia O
authors.initials: JO
authors.lastname: Davydova
authors.affiliation: Bone Marrow Transplant Department, National Research Center for Hematology, Moscow, Russian Federation.
authors.forename: Larisa A
authors.initials: LA
authors.lastname: Kuzmina
authors.affiliation: Flow Cytometry Laboratory, National Research Center for Hematology, Moscow, Russian Federation.
authors.forename: Irina V
authors.initials: IV
authors.lastname: Galtseva
authors.affiliation: Flow Cytometry Laboratory, National Research Center for Hematology, Moscow, Russian Federation.
authors.forename: Nikolay M
authors.initials: NM
authors.lastname: Kapranov
authors.affiliation: Bone Marrow Transplant Department, National Research Center for Hematology, Moscow, Russian Federation.
authors.forename: Vera A
authors.initials: VA
authors.lastname: Vasilyeva
authors.affiliation: Bone Marrow Transplant Department, National Research Center for Hematology, Moscow, Russian Federation.
authors.forename: Darya S
authors.initials: DS
authors.lastname: Dubnyak
authors.affiliation: Bone Marrow Transplant Department, National Research Center for Hematology, Moscow, Russian Federation.
authors.forename: Olga M
authors.initials: OM
authors.lastname: Koroleva
authors.affiliation: Bone Marrow Transplant Department, National Research Center for Hematology, Moscow, Russian Federation.
authors.forename: Ekaterina D
authors.initials: ED
authors.lastname: Mikhalcova
authors.affiliation: Bone Marrow Transplant Department, National Research Center for Hematology, Moscow, Russian Federation.
authors.forename: Natalia N
authors.initials: NN
authors.lastname: Popova
authors.affiliation: Bone Marrow Transplant Department, National Research Center for Hematology, Moscow, Russian Federation.
authors.forename: Elena N
authors.initials: EN
authors.lastname: Parovichnikova
authors.affiliation: Bone Marrow Transplant Department, National Research Center for Hematology, Moscow, Russian Federation.
authors.forename: Valery G
authors.initials: VG
authors.lastname: Savchenko
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000097:blood; D002452:Cell Count; D005260:Female; D006086:Graft vs Host Disease / Q000175:diagnosis*; D053804:Granzymes / Q000032:analysis*; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D008297:Male; D008875:Middle Aged; D050378:T-Lymphocytes, Regulatory / Q000473:pathology*; D013997:Time Factors; D014184:Transplantation, Homologous; D055815:Young Adult
chemical_list: D015415:Biomarkers; C509213:GZMB protein, human; D053804:Granzymes
keywords: Biomarker; Graft-versus-host disease; Granzyme B; Predictor; T-regulatory cells