title: [Establishment and characterization of a laryngeal squamous cell carcinoma cell line].
abstract: Objective:To establish a laryngeal squamous cell carcinoma (LSCC) cell line through primary cell culture and observe its biological characteristics.Methods:Tissue block culture method was used for primary cell culture. After LSCC cells passed 25 timesin vitro,the morphology of cells was observed, keratin was stained histochemically, cell cycle was tested by PI-FACS, and the specie of cells was detected by PCR and short tandem repeat(STR) typing.Results:This newly established LSCC cell line was named as TR-LCC-1, most of the cancer cells were polygonal shape, like the cobblestone, loss of contact inhibition and with overlapping growth. Cell size was large and cell pleomorphism was very obvious. Cytokeratin staining was positive. After 6 months of continuous culturein vitro,the TR-LCC-1 cells passed more than 30 times, and cell doubling time was 201.2h. Cell cycle assay indicated that G1 phase accounted for 51.71%, S phase was 44.56%, and G2 phase was 2.28%. Mycoplasma test showed no mycoplasma contamination. Cell species identification identified TR-LCC-1 was human-derived cells. STR detection showed P26 and P6 were same, and they were different from the STR typing of disclosed cells.Conclusion:The establish ment of the new laryngeal squamous carcinoma cell line TR-LCC-1 can be helpful to the research for laryngeal squamous cell cancer.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China; Key Laboratory of Otorhinolaryngology, Ministry of Education, Beijing 100005, China.
authors.forename: R
authors.initials: R
authors.lastname: Wang
authors.affiliation: Institute of Basic Medical Science, Chinese Academy of Medical Sciences, Beijing 100005, China.
authors.forename: X C
authors.initials: XC
authors.lastname: Bian
authors.affiliation: Institute of Basic Medical Science, Chinese Academy of Medical Sciences, Beijing 100005, China.
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Liu
authors.affiliation: Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology, Beijing 100730, China; Key Laboratory of Otorhinolaryngology, Ministry of Education, Beijing 100005, China; Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
authors.forename: J G
authors.initials: JG
authors.lastname: Fang
authors.affiliation: Key Laboratory of Otorhinolaryngology, Ministry of Education, Beijing 100005, China.
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology, Beijing 100730, China.
authors.forename: H G
authors.initials: HG
authors.lastname: Liu
authors.affiliation: Key Laboratory of Otorhinolaryngology, Ministry of Education, Beijing 100005, China; Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
authors.forename: M
authors.initials: M
authors.lastname: Lian
authors.affiliation: Key Laboratory of Otorhinolaryngology, Ministry of Education, Beijing 100005, China; Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
authors.forename: H Z
authors.initials: HZ
authors.lastname: Ma
authors.affiliation: Key Laboratory of Otorhinolaryngology, Ministry of Education, Beijing 100005, China; Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
authors.forename: L
authors.initials: L
authors.lastname: Feng
authors.affiliation: Key Laboratory of Otorhinolaryngology, Ministry of Education, Beijing 100005, China; Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
authors.forename: H Z
authors.initials: HZ
authors.lastname: Wang
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000473:pathology*; D002453:Cell Cycle; D045744:Cell Line, Tumor / Q000737:chemistry / Q000473:pathology*; D006801:Humans; D007633:Keratins / Q000032:analysis; D007822:Laryngeal Neoplasms / Q000737:chemistry / Q000473:pathology*
chemical_list: D007633:Keratins
keywords: Carcinoma, squamous cell; Cell line; Laryngeal neoplasms; Tumor cells, cultured