title: Curculactones A and B induced the differentiation of C3H10T1/2 and MC3T3-E1 cells to osteoblasts.
abstract: Curculactones A and B are rare γ-lactone derivatives obtained from yellow, natural curcumin following γ-irradiation, and are a type of small molecules with a moderate anti-obesity effect. However, the exact role of curculactones A and B in osteoblast differentiation is unknown. In this study, the effects of curculactones A and B on the differentiation of the mesenchymal cell line C3H10T1/2 and pre-osteoblast cell line MC3T3-E1 to osteoblasts were examined. Curculactones A or B could markedly increase the mRNA levels of osteogenic marker genes and alkaline phosphatase (ALP) activity. Collectively, our findings indicate that curculactones A or B induced osteoblast differentiation through osteogenic expression of genes such as distal-less homeobox 5 (Dlx5), runt-related transcription factor 2 (Runx2), ALP, and osteocalcin (OC).
authors.affiliation: Department of Biotechnology, School of Engineering, Daegu University, Gyeongsan 38453, Republic of Korea; Research Institute of Anti-Aging, Daegu University, Gyeongsan 38453, Republic of Korea.
authors.forename: Hyo-Eun
authors.initials: HE
authors.lastname: Son
authors.affiliation: Department of Food Science and Biotechnology, Daegu University, Gyeongsan 38453, Republic of Korea.
authors.forename: Tae Hoon
authors.initials: TH
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, School of Engineering, Daegu University, Gyeongsan 38453, Republic of Korea; Research Institute of Anti-Aging, Daegu University, Gyeongsan 38453, Republic of Korea. Electronic address: jangwg@daegu.ac.kr.
authors.forename: Won-Gu
authors.initials: WG
authors.lastname: Jang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D007783:Lactones / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D015394:Molecular Structure; D010006:Osteoblasts / Q000166:cytology*; D016133:Polymerase Chain Reaction
chemical_list: D007783:Lactones; C000623113:curculactone A; C000623114:curculactone B
keywords: ALP; Bone; Curculactones A and B; Mesenchymal stem cells; Osteoblast differentiation