title: Talin-1 interaction network promotes hepatocellular carcinoma progression.
abstract: Talin-1 is a known oncogene-associated protein. In this study, we set out to determine its role and mechanisms in hepatocellular carcinoma (HCC) progression. We found Talin-1 to be highly expressed in HCC cells relative to non-cancer liver epithelial cells and to promote tumor growth and metastasis. We used Whole Human Genome Oligo Microarray analysis with HCC cells and HCC cells in which Talin-1 was knocked down using shRNA to identify transcripts regulated by Talin-1. Of the 40,000 tested genes, 3099 were differentially expressed after Talin-1 knockdown; expression of 1924 genes was increased, while expression of 2175 was decreased. Gene ontology (GO) profiling indicated that Talin-1 promotes many HCC progression-related activities, including ion transport and membrane depolarization, cell growth, and cell adhesion. We also characterized the network of gene transcripts regulated by Talin-1 and their role in promoting HCC progression. Our findings confirm the role of Talin-1 in carcinogenesis and provided a set of novel therapeutic targets for the treatment of HCC.
authors.affiliation: Department of General Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.affiliation: Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, China.
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, China.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of General Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, China.
authors.forename: Yechuan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Center Laboratory, First Affiliated Hospital of Anhui Medical University, Hefei, China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of General Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, China.
authors.forename: Yeben
authors.initials: Y
authors.lastname: Qian
mesh_terms: D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D018450:Disease Progression; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D055785:Gene Knockdown Techniques; D053263:Gene Regulatory Networks; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology*; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D016608:Talin / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome
chemical_list: C518832:TLN1 protein, human; D016608:Talin
keywords: Talin-1; hepatocellular carcinoma; ion transport; membrane depolarization; tumor growth and metastasis