title: The Nedd4 binding protein 3 is required for anterior neural development in Xenopus laevis.
abstract: The Fezzin family member Nedd4-binding protein 3 (N4BP3) is known to regulate axonal and dendritic branching. Here, we show that n4bp3 is expressed in the neural tissue of the early Xenopus laevis embryo including the eye, the brain and neural crest cells. Knockdown of N4bp3 in the Xenopus anterior neural tissue results in severe developmental impairment of the eye, the brain and neural crest derived cranial cartilage structures. Moreover, we demonstrate that N4bp3 depletion leads to a significant reduction of both eye and brain specific marker genes and reduced neural crest cell migration. Finally, we demonstrate an impact of N4bp3 deficiency on cell apoptosis and proliferation. Our studies indicate that N4bp3 is required for early anterior neural development of vertebrates. This is in line with a study implicating that genetic disruption of N4BP3 in humans might be related to neurodevelopmental disease.
authors.affiliation: Institute of Biochemistry and Molecular Biology, Ulm University, 89081 Ulm, Germany; International Graduate School in Molecular Medicine Ulm, Ulm University, 89081 Ulm, Germany.
authors.forename: Lena-Maria
authors.initials: LM
authors.lastname: Kiem
authors.affiliation: Institute of Biochemistry and Molecular Biology, Ulm University, 89081 Ulm, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Dietmann
authors.affiliation: Institute of Biochemistry and Molecular Biology, Ulm University, 89081 Ulm, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Linnemann
authors.affiliation: Institute for Anatomy and Cell Biology, Ulm University, 89081 Ulm, Germany; Institute of Anatomy, Division of Neuroanatomy, Otto-von-Guericke University, 39120 Magdeburg, Germany; Leibniz Institute for Neurobiology, 39120 Magdeburg, Germany.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Schmeisser
authors.affiliation: Institute of Biochemistry and Molecular Biology, Ulm University, 89081 Ulm, Germany. Electronic address: susanne.kuehl@uni-ulm.de.
authors.forename: Susanne J
authors.initials: SJ
authors.lastname: Kühl
mesh_terms: D000818:Animals; D017209:Apoptosis; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000196:embryology / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002356:Cartilage / Q000196:embryology / Q000378:metabolism; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism; D047108:Embryonic Development / Q000235:genetics; D005123:Eye / Q000196:embryology / Q000378:metabolism; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D020287:In Situ Nick-End Labeling; D009420:Nervous System / Q000196:embryology* / Q000378:metabolism*; D009432:Neural Crest / Q000166:cytology; D013194:Staining and Labeling; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis / Q000196:embryology* / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D002352:Carrier Proteins; C000619979:Nedd4 binding protein 3, Xenopus; D029867:Xenopus Proteins
keywords: Brain development; Cranial cartilage development; Eye development; N4bp3; Neural crest cells; Xenopus laevis