title: Silencing miR-106b accelerates osteogenesis of mesenchymal stem cells and rescues against glucocorticoid-induced osteoporosis by targeting BMP2.
abstract: Osteoporosis is a serious health problem worldwide. MicroRNA is a post-transcriptional regulator of gene expression by either promoting mRNA degradation or interfering with mRNA translation of specific target genes. It plays a significant role in the pathogenesis of osteoporosis. Here, we first demonstrated that miR-106b (miR-106b-5p) negatively regulated osteogenic differentiation of mesenchymal stem cells in vitro. Then, we found that miR-106b expression increased in C57BL/6 mice with glucocorticoid-induced osteoporosis (GIOP), and that silencing of miR-106b signaling protected mice against GIOP through promoting bone formation and inhibiting bone resorption. At last, we showed that miR-106b inhibited osteoblastic differentiation and bone formation partly through directly targeting bone morphogenetic protein 2 (BMP2) both in vitro and in the GIOP model. Together, our findings have identified the role and mechanism of miR-106b in negatively regulating osteogenesis. Inhibition of miR-106b might be a potential new strategy for treating osteoporosis and bone defects.
authors.affiliation: Orthopedic Department, the First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, No.188 Shizi Street, Suzhou 215006, PR China.
authors.forename: Ke
authors.initials: K
authors.lastname: Liu
authors.affiliation: Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, No.163 Xianlin Avenue, Nanjing 210093, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Jing
authors.affiliation: Orthopedic Department, the First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, No.188 Shizi Street, Suzhou 215006, PR China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Orthopedic Department, the First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, No.188 Shizi Street, Suzhou 215006, PR China.
authors.forename: Xuejie
authors.initials: X
authors.lastname: Fu
authors.affiliation: Orthopedic Department, the First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, No.188 Shizi Street, Suzhou 215006, PR China.
authors.forename: Huan
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Orthopedic Department, the First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, No.188 Shizi Street, Suzhou 215006, PR China.
authors.forename: Xichao
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Orthopedic Department, the First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, No.188 Shizi Street, Suzhou 215006, PR China.
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Tao
authors.affiliation: Orthopedic Department, the First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, No.188 Shizi Street, Suzhou 215006, PR China.
authors.forename: Huilin
authors.initials: H
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, No.163 Xianlin Avenue, Nanjing 210093, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, No.163 Xianlin Avenue, Nanjing 210093, PR China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zen
authors.affiliation: Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, No.163 Xianlin Avenue, Nanjing 210093, PR China. Electronic address: cyzhang@nju.edu.cn.
authors.forename: Chenyu
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, No.163 Xianlin Avenue, Nanjing 210093, PR China. Electronic address: donghaili@nju.edu.cn.
authors.forename: Donghai
authors.initials: D
authors.lastname: Li
authors.affiliation: Orthopedic Department, the First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, No.188 Shizi Street, Suzhou 215006, PR China. Electronic address: shiqin@suda.edu.cn.
authors.forename: Qin
authors.initials: Q
authors.lastname: Shi
mesh_terms: D000818:Animals; D001483:Base Sequence; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D001862:Bone Resorption / Q000150:complications / Q000473:pathology; D002454:Cell Differentiation / Q000528:radiation effects; D003907:Dexamethasone / Q000009:adverse effects; D005260:Female; D055785:Gene Knockdown Techniques; D020868:Gene Silencing / Q000187:drug effects; D005938:Glucocorticoids / Q000009:adverse effects*; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D010012:Osteogenesis / Q000187:drug effects; D010024:Osteoporosis / Q000139:chemically induced* / Q000150:complications / Q000235:genetics* / Q000473:pathology; D010920:Placenta / Q000166:cytology; D011247:Pregnancy; D051785:Smad Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D055396:Bone Morphogenetic Protein 2; D005938:Glucocorticoids; D035683:MicroRNAs; C543896:Mirn106 microRNA, mouse; D051785:Smad Proteins; D003907:Dexamethasone
keywords: BMP2; Glucocorticoid-induced osteoporosis; Mesenchymal stem cells; Osteogenesis; miR-106b