title: High-sensitive surface plasmon resonance microRNA biosensor based on streptavidin functionalized gold nanorods-assisted signal amplification.
abstract: Herein, a facile and sensitive microRNA (miRNA) biosensor was designed by using interfacial biotinylated thiolated DNA molecular beacon (MB) as probe and streptavidin functionalized gold nanorods (Stre-GNRs) as tag for the enhanced surface plasmon resonance (SPR) signal. The MB probe with two terminals labeled with biotin and thiol groups, respectively, was modified on the gold film via thiol-gold interaction. Upon hybridization with the target, the biotinylated group became accessible to the Stre-GNRs. The introduction of the Stre-GNRs tag to the gold film produced strong SPR signal for detection. Our work has illustrated that the plasmonic field extension generated from the gold film to GNRs and the mass increase due to the GNRs have led to drastic sensitivity enhancement. Under optimal conditions, this proposed approach allowed detection of miRNA with the limit of detection (LOD) down to 0.045 pM. The results have shown that the MB probe functionalized sensing film, together with streptavidin-conjugated GNRs, was readily served as a plasmonic coupling partner that can be used as a powerful ultrasensitive sandwich assay for miRNA detection, and GNRs were readily served as promising amplification labels in SPR sensing technology.
authors.affiliation: Research Center for Bioengineering and Sensing Technology, School of Chemistry and Bioengineering, University of Science & Technology Beijing, Beijing, 100083, PR China.
authors.forename: Kaihong
authors.initials: K
authors.lastname: Hao
authors.affiliation: Research Center for Bioengineering and Sensing Technology, School of Chemistry and Bioengineering, University of Science & Technology Beijing, Beijing, 100083, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: He
authors.affiliation: School of Space and Environment, Beihang University, Beijing, 100191, PR China.
authors.forename: Huiting
authors.initials: H
authors.lastname: Lu
authors.affiliation: Research Center for Bioengineering and Sensing Technology, School of Chemistry and Bioengineering, University of Science & Technology Beijing, Beijing, 100083, PR China.
authors.forename: Shaotao
authors.initials: S
authors.lastname: Pu
authors.affiliation: Research Center for Bioengineering and Sensing Technology, School of Chemistry and Bioengineering, University of Science & Technology Beijing, Beijing, 100083, PR China.
authors.forename: Yingnan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Research Center for Bioengineering and Sensing Technology, School of Chemistry and Bioengineering, University of Science & Technology Beijing, Beijing, 100083, PR China. Electronic address: hfdong@ustb.edu.cn.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Dong
authors.affiliation: Research Center for Bioengineering and Sensing Technology, School of Chemistry and Bioengineering, University of Science & Technology Beijing, Beijing, 100083, PR China. Electronic address: zhangxueji@ustb.edu.cn.
authors.forename: Xueji
authors.initials: X
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques; D006046:Gold; D035683:MicroRNAs / Q000032:analysis*; D043942:Nanotubes; D019809:Streptavidin / Q000737:chemistry*; D020349:Surface Plasmon Resonance
chemical_list: D035683:MicroRNAs; D006046:Gold; D019809:Streptavidin
keywords: MicroRNA detection; Signal amplification; Streptavidinylated gold nanorods; Surface plasmon resonance (SPR)