title: Development and characterization of EST-SSR markers forArtocarpus hypargyreus(Moraceae).
abstract: OBJECTIVE
  Polymorphic microsatellite markers were developed forArtocarpus hypargyreus(Moraceae), a threatened species endemic to China, to investigate the genetic diversity and structure of the species.
  
  
  RESULTS
  Based on the transcriptome data ofA. hypargyreus, 63 primer pairs were preliminarily designed and tested, of which 34 were successfully amplified and 10 displayed clear polymorphisms across the 67 individuals from four populations ofA. hypargyreus. The results showed the number of alleles per locus ranged from three to 10, and the observed heterozygosity and expected heterozygosity per locus varied from 0.000 to 0.706 and from 0.328 to 0.807, respectively.
  
  
  CONCLUSIONS
  These microsatellite markers will be useful in exploring genetic diversity and structure ofA. hypargyreus. Furthermore, most loci were successfully cross-amplified inA. nitidusandA. heterophyllus, indicating that they will be of great value for genetic study across this genus.
authors.affiliation: Rescue Center of Wildlife in Shenzhen, Shenzhen 518040, People's Republic of China.
authors.forename: Haijun
authors.initials: H
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Weizheng
authors.initials: W
authors.lastname: Tan
authors.affiliation: Rescue Center of Wildlife in Shenzhen, Shenzhen 518040, People's Republic of China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Kaikai
authors.initials: K
authors.lastname: Meng
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Liao
keywords: Artocarpus hypargyreus; Moraceae; microsatellite marker; transcriptome