title: A Protocol for Large-Scale Proteomic Analysis of Microdissected Formalin Fixed and Paraffin Embedded Tissue.
abstract: Extensive use of biomarkers in diagnostic and therapeutic procedures is probably the hallmark of future medicine. Biomarkers can be of different chemical nature-most frequently DNA, RNA, and proteins are considered as molecules of interest. As the most rational approach appears analysis of proteins, because these are the "effector" molecules directly involved for cell homeostasis. There is no commonly accepted standard procedure in proteomic-based biomarker search. In this chapter, we described a protocol for proteomic analysis of formalin-fixed and paraffin-embedded tissue, which has been proven to be highly effective for proteomics-based biomarker discovery.
authors.affiliation: Wrocław Medical University, Wrocław, Poland.
authors.forename: P
authors.initials: P
authors.lastname: Ostasiewicz
authors.affiliation: Biochemical Proteomics Group, Max-Planck-Institute of Biochemistry, Martinsried, Germany. Electronic address: jwisniew@biochem.mpg.de.
authors.forename: J R
authors.initials: JR
authors.lastname: Wiśniewski
mesh_terms: D015415:Biomarkers; D004247:DNA / Q000737:chemistry; D005557:Formaldehyde; D016612:Paraffin Embedding / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D012313:RNA / Q000737:chemistry
chemical_list: D015415:Biomarkers; D005557:Formaldehyde; D012313:RNA; D004247:DNA
keywords: FASP; FFPE; Filter-aided sample preparation; Formalin fixed and paraffin embedded; MED FASP; Sample preparation