title: The Correlation Between In Vitro Antioxidant Activity and Immunomodulatory Activity of Enzymatic Hydrolysates from Selenium-Enriched Rice Protein.
abstract: The antioxidant and immunomodulatory activities of selenium-enriched rice protein hydrolysates (Se-PH) were evaluated by a cellular antioxidant activity test and macrophage proliferation and phagocytosis assays, respectively. The results showed that trypsin hydrolysate provided the highest proliferation rate of 60.91% at a concentration of 100 μg/mL. Moreover, a remarkable rise in the phagocytosis rates for trypsin hydrolysate (64.1%) and pepsin-trypsin hydrolysate (54.5%) was observed when the sample concentrations were increased to 50 μg/mL. A positive correlation was found between the phagocytic ability of macrophages and both the selenium concentration and the degree of hydrolysis of Se-PH, and the correlation coefficients R obtained were 0.792 and 0.930 (P < 0.05), respectively. The capacity of Se-PH to inhibit the oxidation of dichlorodihydrofluorescein had a significant negative correlation with the phagocytic ability of macrophages (R = -0.840, P < 0.05). In conclusion, a positive correlation was found between the antioxidant activity and the immunomodulatory activity of Se-PH, which could be used as potential functional food additives for improving human health.
authors.affiliation: College of Food Science and Engineering, Nanjing Univ. of Finance and Economics/The Jiangsu Province Center of Cooperative Innovation for Modern Grain Circulation and Security, Nanjing, 210023, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Fang
authors.affiliation: College of Food Science and Engineering, Nanjing Univ. of Finance and Economics/The Jiangsu Province Center of Cooperative Innovation for Modern Grain Circulation and Security, Nanjing, 210023, People's Republic of China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Food Science and Engineering, Nanjing Univ. of Finance and Economics/The Jiangsu Province Center of Cooperative Innovation for Modern Grain Circulation and Security, Nanjing, 210023, People's Republic of China.
authors.forename: Peizhu
authors.initials: P
authors.lastname: Luo
authors.affiliation: College of Food Science and Engineering, Nanjing Univ. of Finance and Economics/The Jiangsu Province Center of Cooperative Innovation for Modern Grain Circulation and Security, Nanjing, 210023, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Pei
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural Univ., Nanjing, 210095, People's Republic of China.
authors.forename: Benard Muinde
authors.initials: BM
authors.lastname: Kimatu
authors.affiliation: College of Food Science and Technology, Henan Univ. of Technology, Zhengzhou, 450001, China.
authors.forename: Kunlun
authors.initials: K
authors.lastname: Liu
authors.affiliation: College of Food Science and Engineering, Nanjing Univ. of Finance and Economics/The Jiangsu Province Center of Cooperative Innovation for Modern Grain Circulation and Security, Nanjing, 210023, People's Republic of China.
authors.forename: Mengjia
authors.initials: M
authors.lastname: Du
authors.affiliation: College of Food Science and Engineering, Nanjing Univ. of Finance and Economics/The Jiangsu Province Center of Cooperative Innovation for Modern Grain Circulation and Security, Nanjing, 210023, People's Republic of China.
authors.forename: Weifen
authors.initials: W
authors.lastname: Qiu
authors.affiliation: College of Food Science and Engineering, Nanjing Univ. of Finance and Economics/The Jiangsu Province Center of Cooperative Innovation for Modern Grain Circulation and Security, Nanjing, 210023, People's Republic of China.|College of Food Science and Technology, Nanjing Agricultural Univ., Nanjing, 210095, People's Republic of China.
authors.forename: Qiuhui
authors.initials: Q
authors.lastname: Hu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry*; D002460:Cell Line; D006801:Humans; D006868:Hydrolysis; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D012275:Oryza / Q000201:enzymology*; D010084:Oxidation-Reduction; D010587:Phagocytosis; D011492:Protein Hydrolysates / Q000737:chemistry*; D012643:Selenium / Q000737:chemistry*; D014357:Trypsin / Q000737:chemistry
chemical_list: D000975:Antioxidants; D011492:Protein Hydrolysates; D014357:Trypsin; D012643:Selenium
keywords: antioxidant activity; immunomodulatory activity; rice protein; selenium