title: Optimization of the Medium for the Production of Extracellular Amylase by thePseudomonas stutzeriISL B5 Isolated from Municipal Solid Waste.
abstract: The management of municipal solid waste is one of the major problems of the present world. The use of microbial enzymes for sustainable management of the solid waste is the need of the time. In the present study, we have isolated a potent amylase producing strain (ISL B5) from municipal solid waste. The strain was identified asPseudomonas stutzeri (P. stutzeri)both biochemically and by 16S rDNA sequencing. The optimization studies revealed that the strain ISL B5 exhibited maximum activity in the liquid media containing 2% starch (2.77 U/ml), 0.8% peptone (2.77 U/ml), and 0.001% Ca2+ion (2.49 U/ml) under the pH 7.5 (2.59 U/ml), temperature 40°C (2.63 U/ml), and 25 h of incubation period (2.49 U/ml). The highest activity of crude enzyme has also been optimized at the pH 8 (2.49 U/ml).
authors.affiliation: Microbiology and Microbial Biotechnology Laboratory, Department of Botany, University of Gour Banga, Malda, West Bengal 732103, India.
authors.forename: Prajesh
authors.initials: P
authors.lastname: Dutta
authors.affiliation: Microbiology and Microbial Biotechnology Laboratory, Department of Botany, University of Gour Banga, Malda, West Bengal 732103, India.
authors.forename: Akash
authors.initials: A
authors.lastname: Deb
authors.affiliation: Microbiology and Microbial Biotechnology Laboratory, Department of Botany, University of Gour Banga, Malda, West Bengal 732103, India.
authors.forename: Sukanta
authors.identifier: 0000-0002-8590-9517
authors.initials: S
authors.lastname: Majumdar