title: Rhizobium hedysari sp. nov., a novel species isolated from a root nodule of Hedysarum multijugum in China.
abstract: A strain 5-1-2Twas isolated from a root nodule of Hedysarum multijugum collected from Zhangye city, Gansu province, north-west China. Phylogenetic analysis based on the 16S rRNA gene sequence and other housekeeping genes (recA and atpD) indicated that the strain represents a novel species in the genus Rhizobium close to the strain Rhizobium subbaraonis JC85Twith similarities of 98.27, 88.92 and 89.62%, respectively. Strain 5-1-2Tcontained Q-10 as the predominant ubiquinone. Our results showed that the major fatty acids were feature 8 (C18:1ω7c and/or C18:1ω6c; 38.90%). In addition, the DNA-DNA hybridizations with the type strains R. subbaraonis JC85Tand Rhizobium halophytocola YC6881Twere 39.2 ± 2.1 and 44.3 ± 1.9, respectively. Therefore, a novel species Rhizobium hedysari sp. nov. is proposed, and 5-1-2T(=CGMCC1.15677T= NBRC112532T)is designated as the type strain.
authors.affiliation: Key Laboratory of Western China's Resources and Environment Chemistry, Hexi University, Zhangye, 734000, Gansu, China. xulinsjfnubi@163.com.|Institute of Agricultural and Biological Technology, Hexi University, Zhangye, 734000, Gansu, China. xulinsjfnubi@163.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.affiliation: Institute of Agricultural and Biological Technology, Hexi University, Zhangye, 734000, Gansu, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Shi
authors.affiliation: Institute of Agricultural and Biological Technology, Hexi University, Zhangye, 734000, Gansu, China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Li
authors.affiliation: Institute of Agricultural and Biological Technology, Hexi University, Zhangye, 734000, Gansu, China.
authors.forename: Shengan
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Institute of Agricultural and Biological Technology, Hexi University, Zhangye, 734000, Gansu, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D007887:Fabaceae / Q000382:microbiology*; D005227:Fatty Acids / Q000032:analysis; D058889:Molecular Typing / Q000379:methods*; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011938:Rec A Recombinases / Q000235:genetics; D012231:Rhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D014157:Transcription Factors / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014157:Transcription Factors; D011938:Rec A Recombinases
keywords: Hedysarum multijugum; Novel species; Rhizobium