title: Raman spectrum reveals Mesenchymal stem cells inhibiting HL60 cells growth.
abstract: Though some research results reveals that Mesenchymal stem cells (MSCs) have the ability of inhibiting tumor cells proliferation, it remains controversial about the precise interaction mechanism during MSCs and tumor cells co-culture. In this study, combing Raman spectroscopic data and principle component analysis (PCA), the biochemical changes of MSCs or Human promyelocytic leukemia (HL60) cells during their co-culture were presented. The obtained results showed that some main Raman peaks of HL60 assigned to nucleic acids or proteins were greatly higher in intensity in the late stage of co-culture than those in the early stage of co-culture while they were still lower relative to the control group, implicating that the effect of MSCs inhibiting HL60 proliferation appeared in the early stage but gradually lost the inhibiting ability in the late stage of co-culture. Moreover, some other peaks of HL60 assigned to proteins were decreased in intensity in the early stage of co-culture relative to the control group but rebounded to the level similar to the control group in the late stage, showing that the content and structure changes of these proteins might be generated in the early stage but returned to the original state in the late stage of co-culture. As a result, in the early stage of MSCs-HL60 co-culture, along with the level of Akt phosphorylation of HL60 was lowered relative to its control group, the proliferation rate of HL60 cells was decreased. And in the late stage of co-culture, along with the level of Akt phosphorylation was rebounded, the reverse transfer of Raman peaks within 875-880cm-1appeared, thus MSCs lost the ability to inhibit HL60 growth and HL60 proliferation was increased. In addition, it was observed that the peak at 811cm-1, which is a marker of RNA, was higher in intensity in the late stage than that in the control group, indicating that MSCs might be differentiated into myofibroblast-like MSCs. In addition, PCA results also exhibited that the physiological state of MSCs can be separated by the first two main components of PC1 or PC2 easily, and the effect of MSCs inhibiting HL60 growth was greatly associated with the time of co-culture.
authors.affiliation: Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou 510006, Guangdong, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Su
authors.affiliation: Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou 510006, Guangdong, China.
authors.forename: Shaoyin
authors.initials: S
authors.lastname: Fang
authors.affiliation: Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou 510006, Guangdong, China.
authors.forename: Daosen
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou 510006, Guangdong, China.
authors.forename: Qinnan
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou 510006, Guangdong, China.
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Lu
authors.affiliation: College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China.
authors.forename: Jindong
authors.initials: J
authors.lastname: Tian
authors.affiliation: Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou 510006, Guangdong, China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Fan
authors.affiliation: Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou 510006, Guangdong, China. Electronic address: zhongly@scnu.edu.cn.
authors.lastname: LiyunZhong
mesh_terms: D049109:Cell Proliferation; D018920:Coculture Techniques; D018922:HL-60 Cells; D006801:Humans; D007231:Infant, Newborn; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D025341:Principal Component Analysis; D013059:Spectrum Analysis, Raman
keywords: Cells analysis; Human promyelocytic leukemia cells (HL60); Medical optics and biotechnology; Mesenchymal stem cells (MSCs); Principle component analysis (PCA); Raman spectroscopy