title: Live Cell Imaging of Endogenous mRNA Using RNA-Based Fluorescence "Turn-On" Probe.
abstract: Messenger RNA (mRNA) plays a critical role in cellular growth and development. However, there have been limited methods available to visualize endogenous mRNA in living cells with ease. We have designed RNA-based fluorescence "turn-on" probes that target mRNA by fusing an unstable form of Spinach with target-complementary sequences. These probes have been demonstrated to be selective, stable, and capable of targeting various mRNAs for live E. coli imaging.
authors.affiliation: Department of Pharmaceutical Chemistry, University of California, San Francisco , San Francisco, California 94143, United States.
authors.forename: Wei Qiang
authors.initials: WQ
authors.lastname: Ong
authors.affiliation: Graduate Program of Biophysics, University of California, San Francisco , San Francisco, California 94143, United States.
authors.forename: Y Rose
authors.initials: YR
authors.lastname: Citron
authors.affiliation: Department of Pharmaceutical Chemistry, University of California, San Francisco , San Francisco, California 94143, United States.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Sekine
authors.affiliation: Department of Pharmaceutical Chemistry, University of California, San Francisco , San Francisco, California 94143, United States.
authors.forename: Bo
authors.identifier: 0000-0003-1704-4141
authors.initials: B
authors.lastname: Huang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001593:Benzyl Compounds / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry*; D054856:G-Quadruplexes; D048288:Imidazolines / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis* / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: C560120:3,5-difluoro-4-hydroxybenzylidene imidazolinone; D052157:Aptamers, Nucleotide; D001593:Benzyl Compounds; D005456:Fluorescent Dyes; D048288:Imidazolines; D012333:RNA, Messenger