title: Caspase 3, 6, 8, and 9 Gene Expression in Peripheral Blood Leukocytes and Plasma Concentrations of IL-6 and TNF-α in Carriers of Different Polymorphic Marker -174G>C Genotypes of IL6 Gene Associated with the Risk of Nonalcoholic Steatohepatitis.
abstract: We revealed an association of IL6 gene -174G>C polymorphism with the development of nonalcoholic steatohepatitis in the Russian population. The risk is significantly higher in carriers of C allele: OR=1.77 (1.04; 3.02). The effects of -174G>C substitution in IL6 gene involving caspase 9 gene transcripts in peripheral blood leukocytes and on blood content of TNF-α in healthy individuals without clinical manifestations of nonalcoholic steatohepatitis were detected. The content of caspase 9 gene transcripts in peripheral blood leukocytes and plasma level of TNF-α were significantly higher in healthy subjects carrying C allele than in carriers of GG genotype. The levels of caspases 3, 6, 8, and 9 gene transcripts in peripheral blood leukocytes and plasma concentrations of TNF-α in patients with nonalcoholic steatohepatitis did not depend on IL6 genotype by -174G<C marker.
authors.affiliation: Institute of Biology, Karelian Research Center, the Russian Academy of Sciences, Petrozavodsk, Russia. irina7m@yandex.ru.
authors.forename: I V
authors.initials: IV
authors.lastname: Kurbatova
authors.affiliation: Institute of Biology, Karelian Research Center, the Russian Academy of Sciences, Petrozavodsk, Russia.
authors.forename: L V
authors.initials: LV
authors.lastname: Topchieva
authors.affiliation: Petrozavodsk State University, Petrozavodsk, Russia.
authors.forename: O P
authors.initials: OP
authors.lastname: Dudanova
mesh_terms: D000328:Adult; D000483:Alleles; D053148:Caspase 3 / Q000235:genetics / Q000276:immunology; D053178:Caspase 6 / Q000235:genetics / Q000276:immunology; D053181:Caspase 8 / Q000235:genetics / Q000276:immunology; D053453:Caspase 9 / Q000235:genetics* / Q000276:immunology; D005260:Female; D005786:Gene Expression Regulation; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006579:Heterozygote; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics* / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D065626:Non-alcoholic Fatty Liver Disease / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D012426:Russia / Q000453:epidemiology; D015398:Signal Transduction; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000276:immunology
chemical_list: C508600:IL6 protein, human; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; C505407:CASP3 protein, human; C505514:CASP6 protein, human; C505530:CASP8 protein, human; C506433:CASP9 protein, human; D053148:Caspase 3; D053178:Caspase 6; D053181:Caspase 8; D053453:Caspase 9
keywords: caspases; interleukin-6; nonalcoholic steatohepatitis; polymorphism