title: TFII-I and AP2α Co-Occupy the Promoters of Key Regulatory Genes Associated with Craniofacial Development.
abstract: The aim of this study is to define the candidate target genes for TFII-I and AP2α regulation in neural crest progenitor cells.
  
  
  
  The GTF2I and GTF2IRD1 genes encoding the TFII-I family of transcription factors are prime candidates for the Williams-Beuren syndrome, a complex multisystem disorder characterized by craniofacial, skeletal, and neurocognitive deficiencies. AP2α, a product of the TFAP2A gene, is a master regulator of neural crest cell lineage. Mutations in TFAP2A cause branchio-oculo-facial syndrome characterized by dysmorphic facial features and orofacial clefts. In this study, we examined the genome-wide promoter occupancy of TFII-I and AP2α in neural crest progenitor cells derived from in vitro-differentiated human embryonic stem cells.
  
  
  
  Our study revealed that TFII-I and AP2α co-occupy a selective set of genes that control the specification of neural crest cells.
  
  
  
  The data suggest that TFII-I and AP2α may coordinately control the expression of genes encoding chromatin-modifying proteins, epigenetic enzymes, transcription factors, and signaling proteins.
authors.forename: Paige
authors.initials: P
authors.lastname: Miranda
authors.forename: Badam
authors.initials: B
authors.lastname: Enkhmandakh
authors.forename: Dashzeveg
authors.initials: D
authors.lastname: Bayarsaihan
mesh_terms: D019280:Branchio-Oto-Renal Syndrome / Q000235:genetics*; D002478:Cells, Cultured; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D009154:Mutation; D009432:Neural Crest / Q000502:physiology; D011401:Promoter Regions, Genetic; D013234:Stem Cells / Q000502:physiology; D050656:Transcription Factor AP-2 / Q000235:genetics*; D019473:Transcription Factors, TFII / Q000235:genetics*; D018980:Williams Syndrome / Q000235:genetics*
chemical_list: D050656:Transcription Factor AP-2; D019473:Transcription Factors, TFII
keywords: TFAP2A; TFII-I; chromatin immunoprecipitation; craniofacial; embryonic; neural crest; stem cells