title: Role of central opioid on the antinociceptive effect of sulfated polysaccharide from the red seaweed Solieria filiformis in induced temporomandibular joint pain.
abstract: This study aimed to investigate the effect of sulfated polysaccharide from red seaweed Solieria filiformis (Fraction F II) in the inflammatory hypernociception in the temporomandibular joint (TMJ) of rats. Male Wistar rats were pretreated (30min) with a subcutaneous injection (s.c.) of vehicle or FII (0.03, 0.3 or 3.0mg/kg) followed by intra-TMJ injection of 1.5% Formalin or 5-hydroxytryptamine (5-HT, 225μg/TMJ). In other set of experiments rats were pretreated (15min) with an intrathecal injection of the non-selective opioid receptors Naloxone, or μ-opioid receptor antagonist CTOP, or δ-opioid receptor Naltridole hydrochloride, or κ-opioid receptor antagonist Nor-Binaltorphimine (Nor-BNI) followed by injection of FII (s.c.). After 30min, the animals were treated with an intra-TMJ injection of 1.5% formalin. After TMJ treatment, behavioral nociception response was evaluated for a 45-min observation period, animals were terminally anesthetized and periarticular tissue, trigeminal ganglion and subnucleus caudalis (SC) were collected plasma extravasation and ELISA analysis. Pretreatment with F II significantly reduced formalin- and serotonin-induced TMJ nociception, inhibit the plasma extravasation and inflammatory cytokines release induced by 1.5% formalin in the TMJ. Pretreatment with intrathecal injection of Naloxone, CTOP, Naltridole or Nor-BNI blocked the antinociceptive effect of F II in the 1.5% formalin-induced TMJ nociception. In addition, F II was able to significantly increase the β-endorphin release in the subnucleus caudalis. The results suggest that F II has a potential antinociceptive and anti-inflammatory effect in the TMJ mediated by activation of opioid receptors in the subnucleus caudalis and inhibition of the release of inflammatory mediators in the periarticular tissue.
authors.affiliation: Fishing Engineering Course, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Ianna Wivianne Fernandes
authors.initials: IW
authors.lastname: Araújo
authors.affiliation: Healthy Sciences Post-Graduate Degree Program, Federal University of Ceará, Sobral, Ceará, Brazil. Electronic address: helliadachaves@yahoo.com.br.
authors.forename: Hellíada Vasconcelos
authors.initials: HV
authors.lastname: Chaves
authors.affiliation: Healthy Sciences Post-Graduate Degree Program, Federal University of Ceará, Sobral, Ceará, Brazil.
authors.forename: José Mário
authors.initials: JM
authors.lastname: Pachêco
authors.affiliation: Northeast Biotechnology Network (Renorbio), Federal University of Pernambuco, Recife, Brazil.
authors.forename: Danielle Rocha
authors.initials: DR
authors.lastname: Val
authors.affiliation: Faculty of Dentistry, Federal University of Ceará, Sobral, Brazil.
authors.forename: Lorena Vasconcelos
authors.initials: LV
authors.lastname: Vieira
authors.affiliation: Faculty of Medicine, Federal University of Ceará, Sobral, Brazil.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Santos
authors.affiliation: Healthy Sciences Post-Graduate Degree Program, Federal University of Ceará, Sobral, Ceará, Brazil.
authors.forename: Raul Sousa
authors.initials: RS
authors.lastname: Freitas
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza, Ceará, Brazil.
authors.forename: Renata Line
authors.initials: RL
authors.lastname: Rivanor
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza, Ceará, Brazil.
authors.forename: Valdécio Silvano
authors.initials: VS
authors.lastname: Monteiro
authors.affiliation: Department of Physiological Sciences, Laboratory of Orofacial Pain, Piracicaba Dental School, University of Campinas - UNICAMP, Piracicaba, São Paulo, Brazil.
authors.forename: Juliana Trindade
authors.initials: JT
authors.lastname: Clemente-Napimoga
authors.affiliation: Healthy Sciences Post-Graduate Degree Program, Federal University of Ceará, Sobral, Ceará, Brazil.
authors.forename: Mirna Marques
authors.initials: MM
authors.lastname: Bezerra
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza, Ceará, Brazil.
authors.forename: Norma Maria Barros
authors.initials: NM
authors.lastname: Benevides
mesh_terms: D000700:Analgesics / Q000627:therapeutic use*; D000701:Analgesics, Opioid / Q000008:administration & dosage* / Q000009:adverse effects; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D002421:Caudate Nucleus / Q000187:drug effects* / Q000378:metabolism; D004347:Drug Interactions; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy*; D011134:Polysaccharides / Q000737:chemistry / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D011957:Receptors, Opioid / Q000378:metabolism; D012624:Seaweed / Q000276:immunology; D013431:Sulfates / Q000737:chemistry; D013704:Temporomandibular Joint / Q000187:drug effects* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D001615:beta-Endorphin / Q000378:metabolism
chemical_list: D000700:Analgesics; D000701:Analgesics, Opioid; D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D011134:Polysaccharides; D011957:Receptors, Opioid; D013431:Sulfates; D014409:Tumor Necrosis Factor-alpha; D001615:beta-Endorphin
keywords: Inflammation; Marine algae; Nociception; Opioid receptors; Sulfated polysaccharide