title: SKN-1-independent transcriptional activation of glutathione S-transferase 4 (GST-4) by EGF signaling.
abstract: InC. elegansresearch, transcriptional activation of glutathione S-transferase 4 (gst-4) is often used as a read-out for SKN-1 activity. While many heed an assumed non-exclusivity of the GFP reporter signal driven by thegst-4promoter to SKN-1, this is also often ignored. We here show thatgst-4can also be transcriptionally activated by EOR-1, a transcription factor mediating effects of the epidermal growth factor (EGF) pathway. Along with enhancing exogenous oxidative stress tolerance, EOR-1 inde-pendently of SKN-1 increasesgst-4transcription in response to augmented EGF signaling. Our findings caution researchers within theC. eleganscommunity to always rely on sufficient experimental controls when assaying SKN-1 transcriptional activity with agst-4p::gfpreporter, such as SKN-1 loss-of-function mutants and/or additional target genes next togst-4.
authors.affiliation: Department of Biology , KU Leuven , Leuven, Belgium.
authors.forename: Giel
authors.initials: G
authors.lastname: Detienne
authors.affiliation: Department of Biology , KU Leuven , Leuven, Belgium.
authors.forename: Pieter
authors.initials: P
authors.lastname: Van de Walle
authors.affiliation: Department of Biology , KU Leuven , Leuven, Belgium.
authors.forename: Wouter
authors.initials: W
authors.lastname: De Haes
authors.affiliation: Department of Biology , KU Leuven , Leuven, Belgium.
authors.forename: Liliane
authors.initials: L
authors.lastname: Schoofs
authors.affiliation: Department of Biology , KU Leuven , Leuven, Belgium.
authors.forename: Liesbet
authors.initials: L
authors.lastname: Temmerman
keywords: CL2166; EGFR; EOR-1; MRJP1; fluorescence; let-23; oxidative stress; royalactin; transcriptional GFP reporter