title: Modulation of cardiomyocyte activity using pulsed laser irradiated gold nanoparticles.
abstract: Can photothermal gold nanoparticle mediated laser manipulation be applied to induce cardiac contraction? Based on our previous work, we present a novel concept of cell stimulation. A 532 nm picosecond laser was employed to heat gold nanoparticles on cardiomyocytes. This leads to calcium oscillations in the HL-1 cardiomyocyte cell line. As calcium is connected to the contractility, we aimed to alter the contraction rate of native and stem cell derived cardiomyocytes. A contraction rate increase was particularly observed in calcium containing buffer with neonatal rat cardiomyocytes. Consequently, the study provides conceptual ideas for a light based, nanoparticle mediated stimulation system.
authors.affiliation: Biomedical Optics Department, Laser Zentrum Hannover e.V., Hollerithallee 8, 30419 Hannover, Germany; Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, Stadtfelddamm 34, 30625 Hannover, Germany; These authors contributed equally to this publication and should be considered co-first authors.
authors.forename: Lara
authors.initials: L
authors.lastname: Gentemann
authors.affiliation: Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, Stadtfelddamm 34, 30625 Hannover, Germany; Institut für Quantenoptik, Gottfried Wilhelm Leibniz Universität Hannover, Welfengarten 1, 30167 Hannover, Germany; Cluster of Excellence REBIRTH, Hannover, Germany; These authors contributed equally to this publication and should be considered co-first authors.
authors.forename: Stefan
authors.initials: S
authors.lastname: Kalies
authors.affiliation: Cluster of Excellence REBIRTH, Hannover, Germany; Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), REBIRTH - Center for Regenerative Medicine, Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
authors.forename: Michelle
authors.initials: M
authors.lastname: Coffee
authors.affiliation: Biomedical Optics Department, Laser Zentrum Hannover e.V., Hollerithallee 8, 30419 Hannover, Germany.
authors.forename: Heiko
authors.initials: H
authors.lastname: Meyer
authors.affiliation: Biomedical Optics Department, Laser Zentrum Hannover e.V., Hollerithallee 8, 30419 Hannover, Germany.
authors.forename: Tammo
authors.initials: T
authors.lastname: Ripken
authors.affiliation: Biomedical Optics Department, Laser Zentrum Hannover e.V., Hollerithallee 8, 30419 Hannover, Germany; Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, Stadtfelddamm 34, 30625 Hannover, Germany; Institut für Quantenoptik, Gottfried Wilhelm Leibniz Universität Hannover, Welfengarten 1, 30167 Hannover, Germany; Cluster of Excellence REBIRTH, Hannover, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Heisterkamp
authors.affiliation: Cluster of Excellence REBIRTH, Hannover, Germany; Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), REBIRTH - Center for Regenerative Medicine, Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Zweigerdt
authors.affiliation: Biomedical Optics Department, Laser Zentrum Hannover e.V., Hollerithallee 8, 30419 Hannover, Germany; Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, Stadtfelddamm 34, 30625 Hannover, Germany.
authors.forename: Dag
authors.initials: D
authors.lastname: Heinemann
keywords: (000.1430) Biology and medicine; (190.4870) Photothermal effects; (350.4855) Optical tweezers or optical manipulation