title: Minimal residual disease detection in Tunisian B-acute lymphoblastic leukemia based on immunoglobulin gene rearrangements.
abstract: IGH gene rearrangement and IGK-Kde gene deletion can be used as molecular markers for the assessment of B lineage acute lymphoblastic leukemia (B-ALL). Minimal residual disease detected based on those markers is currently the most reliable prognosis factor in B-ALL. The aim of this study was to use clonal IGH/IGK-Kde gene rearrangements to confirm B-ALL diagnosis and to evaluate the treatment outcome of Tunisian leukemic patients by monitoring the minimal residual disease (MRD) after induction chemotherapy. Seventeen consecutive newly diagnosed B-ALL patients were investigated by multiplex PCR assay and real time quantitative PCR according to BIOMED 2 conditions. The vast majority of clonal VH-JH rearrangements included VH3 gene. For IGK deletion, clonal VK1f/6-Kde recombinations were mainly identified. These rearrangements were quantified to follow-up seven B-ALL after induction using patient-specific ASO. Four patients had an undetectable level of MRD with a sensitivity of up to 10-5. This molecular approach allowed identification of prognosis risk group and adequate therapeutic decision. The IGK-Kde and IGH gene rearrangements might be used for diagnosis and MRD monitoring of B-ALL, introduced for the first time in Tunisian laboratories.
authors.affiliation: Research Unit 14 ES 19, Department of Biochemistry, Faculty of Medicine, University of Sousse, Sousse, Tunisia.
authors.forename: S
authors.initials: S
authors.lastname: Besbes
authors.affiliation: Research Unit 14 ES 19, Department of Biochemistry, Faculty of Medicine, University of Sousse, Sousse, Tunisia.
authors.forename: W S
authors.initials: WS
authors.lastname: Hamadou
authors.affiliation: Biological Hematology Department, Centre Hospitalier Universitaire Pontchaillou, Rennes, France.
authors.forename: M L
authors.initials: ML
authors.lastname: Boulland
authors.affiliation: Research Unit 14 ES 19, Department of Biochemistry, Faculty of Medicine, University of Sousse, Sousse, Tunisia.|Clinical Hematology Department, Hospital F. Hached, Sousse, Tunisia.
authors.forename: Y B
authors.initials: YB
authors.lastname: Youssef
authors.affiliation: Clinical Hematology Department, Hospital F. Hached, Sousse, Tunisia.
authors.forename: B
authors.initials: B
authors.lastname: Achour
authors.affiliation: Research Unit 14 ES 19, Department of Biochemistry, Faculty of Medicine, University of Sousse, Sousse, Tunisia.|Clinical Hematology Department, Hospital F. Hached, Sousse, Tunisia.
authors.forename: H
authors.initials: H
authors.lastname: Regaieg
authors.affiliation: Research Unit 14 ES 19, Department of Biochemistry, Faculty of Medicine, University of Sousse, Sousse, Tunisia.|Clinical Hematology Department, Hospital F. Hached, Sousse, Tunisia.
authors.forename: A
authors.initials: A
authors.lastname: Khelif
authors.affiliation: Biological Hematology Department, Centre Hospitalier Universitaire Pontchaillou, Rennes, France.
authors.forename: T
authors.initials: T
authors.lastname: Fest
authors.affiliation: Research Unit 14 ES 19, Department of Biochemistry, Faculty of Medicine, University of Sousse, Sousse, Tunisia.
authors.forename: Z
authors.initials: Z
authors.lastname: Soua
mesh_terms: D000293:Adolescent; D014408:Biomarkers, Tumor / Q000235:genetics*; D002675:Child, Preschool; D005260:Female; D015321:Gene Rearrangement / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D018365:Neoplasm, Residual; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000473:pathology* / Q000628:therapy; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor