title: Wnt/β-catenin signaling activates nephronectin expression in osteoblasts.
abstract: Nephronectin (Npnt), an extracellular matrix protein, is considered to play critical roles as an adhesion molecule in the development and functions of various organs and tissues, such as the kidneys and bone. In the present study, we found that Wnt3a strongly enhanced Npnt mRNA expression in osteoblast-like MC3T3-E1 cells, while it also induced an increase in Npnt gene expression in both time- and dose-dependent manners via the Wnt/β-catenin signaling pathway. These results suggest novel mechanisms for Wnt3a-induced osteoblast proliferation and cell survival via Npnt gene expression.
authors.affiliation: Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan; Department of Oral and Maxillofacial Surgery, Tokyo Medical University, Tokyo, Japan.
authors.forename: Mikiko
authors.initials: M
authors.lastname: Ikehata
authors.affiliation: Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan. Electronic address: yamadaa@dent.showa-u.ac.jp.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yamada
authors.affiliation: Department of Integrative Physiology, Shiga University of Medical Science, Shiga, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Morimura
authors.affiliation: Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan; Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, Tokyo, Japan.
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Itose
authors.affiliation: Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Suzawa
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, Tokyo, Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Shirota
authors.affiliation: Department of Oral and Maxillofacial Surgery, Tokyo Medical University, Tokyo, Japan.
authors.forename: Daichi
authors.initials: D
authors.lastname: Chikazu
authors.affiliation: Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan.
authors.forename: Ryutaro
authors.initials: R
authors.lastname: Kamijo
mesh_terms: D016475:3T3 Cells; D000818:Animals; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism*; D015398:Signal Transduction; D060509:Wnt3A Protein / Q000378:metabolism*; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D016326:Extracellular Matrix Proteins; D060509:Wnt3A Protein; C555511:Wnt3a protein, mouse; D051176:beta Catenin; C432915:nephronectin
keywords: Nephronectin; Wnt3a; β-catenin