title: Characterization of chicken interferon-inducible transmembrane protein-10.
abstract: Interferon-inducible transmembrane protein (IFITM) family proteins are antivirus factors. In the present study, we examined the expression pattern of chicken IFITM10 using quantitative reverse transcription-polymerase chain reaction. In adult chickens, IFITM10 levels were markedly lower than those of IFITM3, which exhibits antivirus activity. On the other hand, IFITM10 was expressed in levels similar to those of IFITM3 in embryonic organs. Primordial germ cells in 2.5-d embryos expressed high levels of IFITM10, which gradually decreased with time. The interferon-α stimulation of embryonic fibroblast cells did not enhance the expression of IFITM10. The forced expression of IFITM10 slightly inhibited the infectivity of the VSV-G-pseudotyped lentiviral vector. Furthermore, cell fusion was inhibited by IFITM10 when HeLa cells transfected with the VSV-G expression vector were treated with low pH buffer. Although it remains unclear whether IFITM10 inhibits viral infections under physiological conditions, these results suggest that chicken IFITM10 exhibits antivirus activity.
authors.affiliation: a Department of Biotechnology, Graduate School of Engineering , Nagoya University , Nagoya , Japan.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Okuzaki
authors.affiliation: a Department of Biotechnology, Graduate School of Engineering , Nagoya University , Nagoya , Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Kidani
authors.affiliation: a Department of Biotechnology, Graduate School of Engineering , Nagoya University , Nagoya , Japan.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Kaneoka
authors.affiliation: a Department of Biotechnology, Graduate School of Engineering , Nagoya University , Nagoya , Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Iijima
authors.affiliation: a Department of Biotechnology, Graduate School of Engineering , Nagoya University , Nagoya , Japan.
authors.forename: Ken-Ichi
authors.initials: KI
authors.lastname: Nishijima
mesh_terms: D000818:Animals; D054357:Chemokine CXCL10 / Q000235:genetics* / Q000378:metabolism*; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental; D005822:Genetic Vectors / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology; D016086:Lentivirus / Q000235:genetics; D008562:Membrane Glycoproteins / Q000235:genetics; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D054357:Chemokine CXCL10; C028391:G protein, vesicular stomatitis virus; D016898:Interferon-alpha; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins
keywords: chicken; interferon; interferon-inducible transmembrane protein 10(IFITM10); virus prevention