title: Evaluations of Intravenous Administration of CD34+ Human Umbilical Cord Blood Cells in a Mouse Model of Neonatal Hypoxic-Ischemic Encephalopathy.
abstract: Several cell therapies have been explored as novel therapeutic strategies for neonatal encephalopathy because the benefits of current treatments are limited. We previously reported that intravenous administration of human umbilical cord blood (hUCB) CD34+ cells (hematopoietic stem cells/endothelial progenitor cells) at 48 h after insult exerts therapeutic effects in neonatal mice with stroke, i.e., permanent middle cerebral artery occlusion. Although neonatal stroke and hypoxic-ischemic encephalopathy (HIE) are grouped under the term "neonatal encephalopathy," their pathogenesis differs. However, little is known about the differences in the effects of the same treatment between these 2 diseases. In this study, we investigated whether the same treatment protocol exerts therapeutic effects in neonatal mice with HIE. The treatment significantly ameliorated the decreased cerebral blood flow in the ischemic penumbra. Although the cylinder and rotarod tests showed a trend of amelioration of behavioral impairments from the treatment, these were not statistically significant. Morphological brain injuries were not altered by treatment. The cell administration did not cause any adverse effects apart from hyperactivity in the open-field test. Some of these findings are consistent with the results obtained in our previous study using a stroke model, but others are not. This study suggests that the treatment protocol needs to be optimized for each pathological condition.
authors.affiliation: Department of Regenerative Medicine and Tissue Engineering, National Cerebral and Cardiovascular Center, Suita, Japan.
authors.forename: Makiko
authors.initials: M
authors.lastname: Ohshima
authors.forename: Akihiko
authors.initials: A
authors.lastname: Taguchi
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Sato
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ogawa
authors.forename: Satoshi
authors.initials: S
authors.lastname: Saito
authors.forename: Kenichi
authors.initials: K
authors.lastname: Yamahara
authors.forename: Masafumi
authors.initials: M
authors.lastname: Ihara
authors.forename: Mariko
authors.initials: M
authors.lastname: Harada-Shiba
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Ikeda
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Matsuyama
authors.forename: Masahiro
authors.initials: M
authors.lastname: Tsuji
mesh_terms: D061605:Administration, Intravenous / Q000379:methods; D000818:Animals; D000831:Animals, Newborn; D018952:Antigens, CD34 / Q000276:immunology; D001927:Brain Diseases / Q000628:therapy*; D002560:Cerebrovascular Circulation / Q000502:physiology; D036101:Cord Blood Stem Cell Transplantation / Q000379:methods; D004195:Disease Models, Animal; D006801:Humans; D020925:Hypoxia-Ischemia, Brain / Q000473:pathology / Q000628:therapy*; D008822:Mice, Transgenic; D020521:Stroke / Q000276:immunology / Q000628:therapy
chemical_list: D018952:Antigens, CD34