title: Characterization of particulate matter binding peptides screened from phage display.
abstract: Particulate matter (PM), especially particulates with diameters of less than 2.5 μm, can penetrate the alveolar region and increase the risk of respiratory diseases. This has stimulated research efforts to develop detection methods so that counter measures can be taken. In this study, four PM binding peptides were obtained by phage display and binding characteristics of these peptides were investigated using the peptide array. The strongest binding peptide, WQDFGAVRSTRS, displayed a binding property, measured in terms of spot intensity, 11.4 times higher than that of the negative control, AAAAA. Inductively coupled plasma mass spectrometry (ICPMS) analysis of the transition metal compounds in the PM bound to the peptide spots was performed, and two peptides showed higher binding towards Cu and Zn compounds in PM. These results suggest that the screened peptides could serve as an indicator of transition metal compounds, which are related to adverse health effects, contained in PM.
authors.affiliation: Department of Chemical Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8552, Japan.
authors.forename: Aw Wei
authors.initials: AW
authors.lastname: Liang Alvin
authors.affiliation: Department of Chemical Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8552, Japan; Office for the Impulsing Paradigm Change Through Disruptive Technologies Program (ImPACT), Japan Science and Technology Agency (JST), Tokyo Headquarters 2 K's Gobancho 7, Gobancho, Chiyoda-ku, Tokyo 102-0076, Japan.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Tanaka
authors.affiliation: Department of Chemical Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8552, Japan; Office for the Impulsing Paradigm Change Through Disruptive Technologies Program (ImPACT), Japan Science and Technology Agency (JST), Tokyo Headquarters 2 K's Gobancho 7, Gobancho, Chiyoda-ku, Tokyo 102-0076, Japan. Electronic address: okochi.m.aa@m.titech.ac.jp.
authors.forename: Mina
authors.initials: M
authors.lastname: Okochi
mesh_terms: D000393:Air Pollutants / Q000737:chemistry / Q000378:metabolism; D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics / Q000378:metabolism*; D003300:Copper / Q000009:adverse effects / Q000032:analysis / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry; D052638:Particulate Matter / Q000009:adverse effects / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D019151:Peptide Library; D010455:Peptides / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015032:Zinc / Q000009:adverse effects / Q000032:analysis / Q000378:metabolism
chemical_list: D000393:Air Pollutants; D052638:Particulate Matter; D019151:Peptide Library; D010455:Peptides; D003300:Copper; D015032:Zinc
keywords: Metal binding peptide; Particulate matter; Peptide array; Phage display; Transition metal