title: Monitoring of adsorption behaviors of bovine serum albumin onto a stainless steel surface by the quartz crystal microbalance based on admittance analysis.
abstract: The adsorption process of bovine serum albumin (BSA) onto a stainless steel surface was investigated using the quartz crystal microbalance based on admittance analysis. The adhered mass change ∆m increased with time as a result of contacting the BSA solution, and considerably long period (>2 h) was required for the attainment of the asymptotic values of ∆m as well as dissipation factor ∆D. The relation between ΔD and Δm suggested that the layer of adsorbed BSA molecules became stiffer with increasing time at higher BSA concentration. The relation between Δm after 2 h and the final BSA concentration was described well by the Langmuir adsorption isotherm. However, the time course of Δm clearly deviated from the Langmuir adsorption model. The stretched exponential function model described the time course of Δm well although it was an empirical one.
authors.affiliation: a Department of Food Science and Technology, Tokyo University of Marine Science and Technology , Tokyo , Japan.
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Hagiwara
authors.affiliation: a Department of Food Science and Technology, Tokyo University of Marine Science and Technology , Tokyo , Japan.
authors.forename: Phosri
authors.initials: P
authors.lastname: Nattawut
authors.affiliation: a Department of Food Science and Technology, Tokyo University of Marine Science and Technology , Tokyo , Japan.
authors.forename: Mario
authors.initials: M
authors.lastname: Shibata
authors.affiliation: a Department of Food Science and Technology, Tokyo University of Marine Science and Technology , Tokyo , Japan.
authors.forename: Takaharu
authors.initials: T
authors.lastname: Sakiyama
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D058525:Quartz Crystal Microbalance Techniques; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry; D013193:Stainless Steel / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D012996:Solutions; D013193:Stainless Steel; D012710:Serum Albumin, Bovine
keywords: QCM-A; bovine serum albumin; dissipation factor; protein adsorption; stretched exponential function