title: ASSOCIATION STUDY BETWEEN POLYMORPHISMS OF THE p53 AND LYMPHOTOXIN ALPHA (LTA) GENES AND THE RISK OF PROLIFERATIVE VITREORETINOPATHY/RETINAL DETACHMENT IN A MEXICAN POPULATION.
abstract: OBJECTIVE
  To report the results of an association study between single-nucleotide polymorphisms of the p53 and LTA genes and the risk of proliferative vitreoretinopathy (PVR)/retinal detachment (RD) in a Mexican cohort.
  
  
  METHODS
  A total of 380 unrelated subjects were studied, including 98 patients with primary rhegmatogenous RD without PVR, 82 patients with PVR after RD surgery, and 200 healthy, ethnically matched subjects. Genotyping of single-nucleotide polymorphisms rs1042522 (p53 gene) and rs2229094 (LTA gene) was performed by direct nucleotide sequencing. Allele frequencies, genotype frequencies, and Hardy-Weinberg equilibrium were assessed with HaploView software.
  
  
  RESULTS
  No significant differences in the allelic distributions of the previously identified risk C allele for LTA rs2229094 were observed between RD subjects and controls (odds ratio [95% confidence interval] = 0.8 [0.5-1.2]; P = 0.3). Conversely, the C allele for rs1042522 in p53 was positively associated with an increased risk for RD (odds ratio [95% confidence interval] = 1.4 [1.01-1.9]; P = 0.04). No significant differences were observed when the subgroup of 82 RD + PVR subjects was compared with the subgroup of 98 patients with RD.
  
  
  CONCLUSIONS
  The C allele for rs1042522 in p53 was genetically associated with a higher risk for RD but not for PVR in this cohort. This is the first association study attempting replication of PVR-associated risk alleles in a nonwhite population.
authors.affiliation: Department of Genetics, Institute of Ophthalmology "Conde de Valenciana," Mexico City, Mexico.
authors.forename: Natalia
authors.initials: N
authors.lastname: Quiroz-Casian
authors.affiliation: Department of Genetics, Institute of Ophthalmology "Conde de Valenciana," Mexico City, Mexico.
authors.forename: David
authors.initials: D
authors.lastname: Lozano-Giral
authors.affiliation: Department of Genetics, Instituto Nacional de Rehabilitación, Mexico City, Mexico.
authors.forename: Antonio
authors.initials: A
authors.lastname: Miranda-Duarte
authors.affiliation: Unit of Biochemistry ITO-UNAM, Oaxaca, Oaxaca, Mexico.
authors.forename: Ivan
authors.initials: I
authors.lastname: Garcia-Montalvo
authors.affiliation: Department of Retina, Institute of Ophthalmology "Conde de Valenciana," Mexico City, Mexico.
authors.forename: Jose L
authors.initials: JL
authors.lastname: Rodriguez-Loaiza
authors.affiliation: Department of Genetics, Institute of Ophthalmology "Conde de Valenciana," Mexico City, Mexico.|Biochemistry Department, Faculty of Medicina, UNAM, Mexico City, Mexico.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Zenteno
mesh_terms: D000368:Aged; D000483:Alleles; D004247:DNA / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D015994:Incidence; D008233:Lymphotoxin-alpha / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D012163:Retinal Detachment / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics*; D012307:Risk Factors; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000378:metabolism; D018630:Vitreoretinopathy, Proliferative / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics*; D014822:Vitreous Body / Q000473:pathology
chemical_list: D008233:Lymphotoxin-alpha; D016159:Tumor Suppressor Protein p53; D004247:DNA