title: Quantification of rutin in rat's brain by UHPLC/ESI-Q-TOF-MS/MS after intranasal administration of rutin loaded chitosan nanoparticles.
abstract: Rutin (RT), an antioxidant drug, has been utilized to treat cerebral ischemia hence a sensitive quantification method for estimation of RT in brain homogenate is necessary to develop. This study aims to prepare RT loaded Chitosan Nanoparticles (RT-CS-NPs) develop and validate ultra-high performance liquid chromatography-electrospray ionization-synapt mass spectrometric method Synapt Mass Spectrometry (Synapt MS) (UHPLC/ESI-QTOF-MS/MS) for quantification of RT in brain homogenate from Wistar rat. The process of chromatographic separation was carried out on Waters ACQUITY UPLC™ with the components of separation in detail as; column: BEH C-18 with dimension as 2.1 mm×100 mm and particle size 1.7 µm, mobile phase: acetonitrile (85 % v/v/v): 2 mM ammonium formate (15 % v/v/v): formic acid (0.1 % v/v/v) and flow rate: 0.25 mL/min. Liquid-liquid extraction method (LLE), in mixture, i.e. ethyl acetate:acetonitrile, was considered to optimize the recovery of analyte from the brain homogenate of Wistar rat. Over a total run time of 5 minutes, the elution time for RT and internal standard (IS), i.e. Tolbutamide, observed was 2.67 and 2.82 min respectively whereas the transition observed for RT and IS was at m/z 611.1023/303.1071 and 271.1263/155.1073, respectively. Results, regarding various processes and parameters studied for RT as summarized, established a linear dynamic range over a concentration range of 1.00 ng/mL - 1000.0 ng/mL with r2; 0.9991±0.0010. Accuracy for intra and inter-assay in terms of % CV revealed a range of 0.45- 2.11 whereas lower limit of detection (LOD) and quantitation (LOQ) observed was 0.09 ng/mL and 0.142 ng/mL, respectively. The analyte stability as well as method specificity and accuracy, i.e. recovery > 86 %, supports the idea for application of current developed method in order to quantify and evaluate the RT-loaded-CS-NPs for RT determination in brain homogenate after intranasal drug delivery.
authors.affiliation: Department of Pharmaceutics, College of Clinical Pharmacy, University of Dammam, Dammam-31441, Kingdom of Saudi Arabia.
authors.forename: Niyaz
authors.initials: N
authors.lastname: Ahmad
authors.affiliation: Department of Natural Products and Alternative Medicine, College of Clinical Pharmacy, University of Dammam, Dammam-31441, Kingdom of Saudi Arabia.
authors.forename: Rizwan
authors.initials: R
authors.lastname: Ahmad
authors.affiliation: Department of Pharmacy Practice, College of Clinical Pharmacy, University of Dammam, Dammam 31441, Kingdom of Saudi Arabia.
authors.forename: Atta Abbas
authors.initials: AA
authors.lastname: Naqvi
authors.affiliation: Department of Pharmaceutics, School of Medical and Allied Sciences, Galgotias University, Gautam Budh Nagar, Greater Noida-201310, India.
authors.forename: Md Aftab
authors.initials: MA
authors.lastname: Alam
authors.affiliation: Department of Chemistry, Faculty of Science, Hamdard University, New Delhi-110062, India.
authors.forename: Mohd
authors.initials: M
authors.lastname: Samim
authors.affiliation: Nanomedicine Lab, Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi-110062, India.
authors.forename: Zeenat
authors.initials: Z
authors.lastname: Iqbal
authors.affiliation: Nanomedicine Lab, Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi-110062, India.
authors.forename: Farhan Jalees
authors.initials: FJ
authors.lastname: Ahmad
keywords: UHPLC-MS/MS-ESI-Q-TOF; brain pharmacokinetic; chitosan nanoparticles; method development and validation; rutin