title: Establishment and characterization of a heart-derived cell line from goldfish (Carassius auratus).
abstract: The goldfish Carassius auratus, a freshwater fish in the family Cyprinidae, was one of the earliest fish to be domesticated for ornamental purposes. A cell line was established from goldfish heart (GH) tissue to create a biological monitoring tool for viral diseases. The GH cell line was optimally maintained at 25 °C in M199 medium supplemented with 10-20% fetal bovine serum. A chromosomal analysis indicated that the cell line remained diploid, with a mean chromosomal count of 100. In viral inoculation assays, significant cytopathic effects (CPEs) were caused by epizootic hematopoietic necrosis virus (EHNV), Andrias davidianus iridovirus (ADIV), and Bohle iridovirus (BIV) infections in the fish cells and the viral titers (average value) of EHNV, ADIV, and BIV in GH cells reached 105.0, 104.5, and 105.0TCID50/0.1 mL, respectively, within 7 days. However, no CPE was observed in the cells infected with viral hemorrhagic septicemia virus (VHSV), infectious hematopoietic necrosis virus (IHNV), spring viremia of carp virus (SVCV), infectious pancreatic necrosis virus (IPNV), channel catfish virus (CCV), or grass carp reovirus (GCRV). These results suggest that the GH cell line is a valuable tool for studying viral pathogenesis.
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing, 100029, China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Jing
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing, 100029, China.
authors.forename: Xiangmei
authors.initials: X
authors.lastname: Lin
authors.affiliation: Beijing Aquaculture Technology Extension Station, Beijing, 100021, China.
authors.forename: Lipu
authors.initials: L
authors.lastname: Xu
authors.affiliation: She kou in shen zhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518067, China.
authors.forename: Longying
authors.initials: L
authors.lastname: Gao
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing, 100029, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing, 100029, China.
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing, 100029, China. sqwu@sina.com.
authors.forename: Shaoqiang
authors.initials: S
authors.lastname: Wu
mesh_terms: D000818:Animals; D002460:Cell Line; D006054:Goldfish / Q000033:anatomy & histology*; D017994:Iridovirus / Q000502:physiology; D009206:Myocardium / Q000166:cytology*; D032383:Myocytes, Cardiac / Q000502:physiology* / Q000821:virology; D014779:Virus Replication / Q000502:physiology; D014780:Viruses / Q000145:classification
keywords: Carassius auratus; Cell line; Goldfish; Heart; Transfection; Viral susceptibility