title: IsomiR expression patterns in canonical and Dicer‑independent microRNAs.
abstract: Multiple microRNA (miRNA) variants, known as isomiRs, are extensively distributed in miRNA loci and predominantly derive from the alternative cleavage of Drosha/Dicer and 3'addition events. The present study aimed to investigate the expression patterns of multiple isomiRs in typical miRNA and Dicer‑independent miRNA loci by conducting evolutionary and expression analysis using public datasets. Although different miRNA maturation processes exist, multiple isomiRs can be detected by similar expression distributions. However, isomiR expression in Dicer‑independent miRNA loci tends to be at a moderate level, particularly for random distribution in the ends that are split by Dicer in the typical miRNA loci. Compared with the mature miRNA locus (dominant miRNA locus), the non‑dominant miRNA locus indicates an expression distribution similar to that of the Dicer‑independent miRNA locus. These results increase the understanding of multiple isomiRs in the progression of diseases.
authors.affiliation: Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing, Jiangsu 210023, P.R. China.
authors.forename: Tingming
authors.initials: T
authors.lastname: Liang
authors.affiliation: Shandong Provincial Key Laboratory of Functional Macromolecular Biophysics, Institute of Biophysics, Dezhou University, Dezhou, Shandong 253023, P.R. China.
authors.forename: Jiafeng
authors.initials: J
authors.lastname: Yu
authors.affiliation: Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing, Jiangsu 210023, P.R. China.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Bioinformatics, School of Geographic and Biologic Information, Nanjing University of Posts and Telecommunications, Nanjing, Jiangsu 210023, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Guo
mesh_terms: D030561:Databases, Nucleic Acid; D019143:Evolution, Molecular; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D056426:Genetic Loci; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D010802:Phylogeny; D059467:Transcriptome
chemical_list: D035683:MicroRNAs