title: Evolutionary plasticity of plasma membrane interaction in DREPP family proteins.
abstract: The plant-specific DREPP protein family comprises proteins that were shown to regulate the actin and microtubular cytoskeleton in a calcium-dependent manner. Our phylogenetic analysis showed that DREPPs first appeared in ferns and that DREPPs have a rapid and plastic evolutionary history in plants. Arabidopsis DREPP paralogues called AtMDP25/PCaP1 and AtMAP18/PCaP2 are N-myristoylated, which has been reported as a key factor in plasma membrane localization. Here we show that N-myristoylation is neither conserved nor ancestral for the DREPP family. Instead, by using confocal microscopy and a new method for quantitative evaluation of protein membrane localization, we show that DREPPs rely on two mechanisms ensuring their plasma membrane localization. These include N-myristoylation and electrostatic interaction of a polybasic amino acid cluster. We propose that various plasma membrane association mechanisms resulting from the evolutionary plasticity of DREPPs are important for refining plasma membrane interaction of these signalling proteins under various conditions and in various cells.
authors.affiliation: Department of Experimental Plant Biology, Faculty of Science, Charles University, Viničná 5, 128 44 Prague 2, Czech Republic. Electronic address: vosolsob@natur.cuni.cz.
authors.forename: Stanislav
authors.initials: S
authors.lastname: Vosolsobě
authors.affiliation: Department of Experimental Plant Biology, Faculty of Science, Charles University, Viničná 5, 128 44 Prague 2, Czech Republic. Electronic address: Petrasek@ueb.cas.cz.
authors.forename: Jan
authors.initials: J
authors.lastname: Petrášek
authors.affiliation: Department of Experimental Plant Biology, Faculty of Science, Charles University, Viničná 5, 128 44 Prague 2, Czech Republic. Electronic address: schwarze@natur.cuni.cz.
authors.forename: Kateřina
authors.initials: K
authors.lastname: Schwarzerová
mesh_terms: D005075:Biological Evolution; D002462:Cell Membrane / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry*; D019814:Myristic Acid / Q000378:metabolism; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry*; D055672:Static Electricity
chemical_list: D008565:Membrane Proteins; D010940:Plant Proteins; D019814:Myristic Acid
keywords: AtMDP25/PCaP1; DREPP; N-myristoylation; Nicotiana tabacum; Polybasic cluster