title: A new update for radiocontrast-induced nephropathy aggravated with glycerol in rats: the protective potential of epigallocatechin-3-gallate.
abstract: Contrast media (CM) is known to have nephrotoxic adverse effects. Epigallocatechin-3-gallate (EGCG) is the most abundant and active catechin in green tea, and has strong antioxidant and anti-inflammatory properties. This study investigated whether EGCG can reduce contrast-induced nephrotoxicity (CIN), alone or with glycerol (GLY)-induced renal damage, and to understand its mechanisms of protection against toxicity, using models of GLY and CIN in rats. The rats were separated into eight groups (n = 6 in each), as follows: Healthy, GLY, CM, GLY + CM, CM + EGCG 50 mg/kg (po), GLY + CM + EGCG 50 mg/kg (po), CM + EGCG 100 mg/kg (po), and GLY + CM + EGCG 100 mg/kg (po). Both doses of EGCG protected against CM-induced renal dysfunction, as measured by serum creatinine and blood urea nitrogen (BUN). In addition, EGCG treatment markedly improved CIN-induced oxidative stress, and resulted in a significant down-regulatory effect on tumor necrosis factor (TNF)-α and nuclear factor (NF)-κB mRNA expression. Moreover, histopathological analysis showed that EGCG also attenuated CM-induced kidney damage. Considering the potential clinical use of CM and the numerous health benefits of EGCG, this study showed the protective role of multi-dose EGCG treatment on CIN and GLY-aggravated CIN through different mechanisms.
authors.affiliation: a Faculty of Pharmacy, Pharmaceutical Toxicology Department , Ataturk University , Erzurum , Turkey.
authors.forename: Saziye Sezin
authors.initials: SS
authors.lastname: Palabiyik
authors.affiliation: b Faculty of Medicine, Pharmacology Department , Ataturk University , Erzurum , Turkey.|c Faculty of Pharmacy, Pharmacology Department , Erzincan University , Erzincan , Turkey.
authors.forename: Busra
authors.initials: B
authors.lastname: Dincer
authors.affiliation: b Faculty of Medicine, Pharmacology Department , Ataturk University , Erzurum , Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Cadirci
authors.affiliation: b Faculty of Medicine, Pharmacology Department , Ataturk University , Erzurum , Turkey.
authors.forename: Irfan
authors.initials: I
authors.lastname: Cinar
authors.affiliation: d Faculty of Medicine, Pathology Department , Ataturk University , Erzurum , Turkey.
authors.forename: Cemal
authors.initials: C
authors.lastname: Gundogdu
authors.affiliation: e Faculty of Pharmacy, Pharmacology Department , Ataturk University , Erzurum , Turkey.
authors.forename: Beyzagul
authors.initials: B
authors.lastname: Polat
authors.affiliation: f Faculty of Medicine, Pharmacology Department , Kafkas University , Kars , Turkey.
authors.forename: Muhammed
authors.initials: M
authors.lastname: Yayla
authors.affiliation: b Faculty of Medicine, Pharmacology Department , Ataturk University , Erzurum , Turkey.
authors.forename: Zekai
authors.initials: Z
authors.lastname: Halici
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001806:Blood Urea Nitrogen; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D003287:Contrast Media / Q000009:adverse effects*; D016207:Cytokines / Q000097:blood; D005990:Glycerol / Q000009:adverse effects*; D007668:Kidney / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced* / Q000188:drug therapy*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013662:Tea
chemical_list: D000975:Antioxidants; D003287:Contrast Media; D016207:Cytokines; D013662:Tea; D002392:Catechin; C045651:epigallocatechin gallate; D005990:Glycerol
keywords: Contrast media; EGCG; nephrotoxicity; rat