title: Urea-formaldehyde monolithic column for hydrophilic in-tube solid-phase microextraction of aminoglycosides.
abstract: A novel urea-formaldehyde (UF) monolithic column has been developed and exploited as a sorbent for hydrophilic in-tube solid-phase microextraction (in-tube SPME) of aminoglycosides (AGs). Because of the innate hydrophilicity, UF monolith showed high extraction efficiency towards these hydrophilic analytes. The adsorption capacities for target compounds dissolved in water/ACN (1:1, v/v) were in the range of 5.18-7.36μg/cm. Due to the lack of a chromophore, evaporative light scattering detector (ELSD) was selected as the detector for AGs, and coupled with the online in-tube SPME-HPLC system. Several factors of the online system, such as trifluoroacetic acid (TFA) and ACN percentage in the sampling solution, ionic strength in the sample solution, elution volume, sampling and elution flow rate, were optimized with respect to the extraction efficiencies. Under the optimized conditions, the limits of detection (LODs) of streptomycin, tobramycin and neomycin were discovered in the range of 3.0-5.2μg/kg. The recoveries were ranged from 82.1 to 96.7% with relative standard deviations (RSDs) of 2.3-5.1% (n=4) at spiking levels of 50, 200 and 500μg/kg, respectively. The excellent applicability of the UF monolithic column was examined by the determination of streptomycin in practical tilapia samples, which showed the potential advantages for the analysis of polar analytes in complicated samples.
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China. Electronic address: jbwang@fzu.edu.cn.
authors.forename: Jiabin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Jiang
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China.
authors.forename: Wenbang
authors.initials: W
authors.lastname: Li
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: Institute of Food Safety and Environment Monitoring, Fuzhou University, Fuzhou, 350108, China.
authors.forename: Xucong
authors.initials: X
authors.lastname: Lin
authors.affiliation: Institute of Food Safety and Environment Monitoring, Fuzhou University, Fuzhou, 350108, China.
authors.forename: Zenghong
authors.initials: Z
authors.lastname: Xie
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: You
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China; Key Laboratory of Biomedical Material of Tianjin, Institute of Biomedical Engineering, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192, China. Electronic address: zhangqiq@126.com.
authors.forename: Qiqing
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D000617:Aminoglycosides / Q000302:isolation & purification*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D057927:Hydrophobic and Hydrophilic Interactions; D057230:Limit of Detection; D009355:Neomycin / Q000302:isolation & purification; D011108:Polymers / Q000138:chemical synthesis; D052617:Solid Phase Microextraction / Q000295:instrumentation / Q000379:methods*; D013307:Streptomycin / Q000302:isolation & purification; D017210:Tilapia; D014031:Tobramycin / Q000302:isolation & purification; D014867:Water
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D011108:Polymers; D014867:Water; D009355:Neomycin; D014031:Tobramycin; D013307:Streptomycin
keywords: Aminoglycosides; Evaporative light scattering detector; Hydrophilic interaction; In-tube solid-phase microextraction; Urea-formaldehyde monolithic column