title: Serum and follicular fluid monocyte chemotactic protein-1 levels are elevated in obese women and are associated with poorer clinical pregnancy rate after in vitro fertilization: a pilot study.
abstract: To determine whether monocyte chemotactic protein-1 (MCP-1), a proinflammatory chemokine important in ovulation, is abnormally elevated in obese women undergoing IVF and whether serum and follicular fluid (FF) levels of MCP-1 are associated with IVF outcome.
  
  
  
  Prospective pilot study.
  
  
  
  Academic center.
  
  
  
  Women undergoing IVF.
  
  
  
  Serum and FF were collected from women undergoing IVF.
  
  
  
  Correlation between MCP-1 and other inflammatory markers with adiposity and pregnancy outcome after IVF.
  
  
  
  Obese women had significantly higher serum and FF MCP-1 levels compared with overweight and normal weight women. Serum MCP-1, granulocyte colony stimulating factor, catalase, and C-reactive protein (CRP) were positively correlated with body mass index (BMI). After adjusting for age and baseline FSH, these correlations remained significant for serum MCP-1, granulocyte colony stimulating factor, and CRP. In the FF, only MCP-1 was positively correlated with BMI. Women who became pregnant had significantly lower serum MCP-1 and CRP levels compared with those who did not become pregnant; this difference was more pronounced among women with diminished ovarian reserve. Receiver operating characteristic curve demonstrated that serum MCP-1 levels >373.0 pg/mL in all women and >362.6pg/mL in women with diminished ovarian reserve predicted failure to achieve a clinical pregnancy.
  
  
  
  Elevations in serum and FF MCP-1 levels are positively correlated with adiposity and negatively correlated with pregnancy rates (PRs) in women undergoing IVF.
authors.affiliation: Montefiore's Institute for Reproductive Medicine and Health, Bronx, New York; Department of Obstetrics, Gynecology and Women's Health, Division of Reproductive Endocrinology and Infertility, Albert Einstein College of Medicine, Bronx, New York. Electronic address: erbuyuk@yahoo.com.
authors.forename: Erkan
authors.initials: E
authors.lastname: Buyuk
authors.affiliation: Montefiore's Institute for Reproductive Medicine and Health, Bronx, New York; Department of Obstetrics, Gynecology and Women's Health, Division of Reproductive Endocrinology and Infertility, Albert Einstein College of Medicine, Bronx, New York.
authors.forename: Obehi A
authors.initials: OA
authors.lastname: Asemota
authors.affiliation: Department of Obstetrics and Gynecology, Reproductive Endocrinology and Infertility, NYU School of Medicine, New York, New York.
authors.forename: Zaher
authors.initials: Z
authors.lastname: Merhi
authors.affiliation: Department of Obstetrics, Gynecology and Women's Health, Division of Reproductive Endocrinology and Infertility, Albert Einstein College of Medicine, Bronx, New York; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York; Department of Endocrinology, Albert Einstein College of Medicine, Bronx, New York.
authors.forename: Maureen J
authors.initials: MJ
authors.lastname: Charron
authors.affiliation: Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, University of Pennsylvania, Philadelphia, Pennsylvania.
authors.forename: Dara S
authors.initials: DS
authors.lastname: Berger
authors.affiliation: Montefiore's Institute for Reproductive Medicine and Health, Bronx, New York.
authors.forename: Athena
authors.initials: A
authors.lastname: Zapantis
authors.affiliation: Montefiore's Institute for Reproductive Medicine and Health, Bronx, New York; Department of Obstetrics, Gynecology and Women's Health, Division of Reproductive Endocrinology and Infertility, Albert Einstein College of Medicine, Bronx, New York.
authors.forename: Sangita K
authors.initials: SK
authors.lastname: Jindal
mesh_terms: D050154:Adiposity; D000328:Adult; D019540:Area Under Curve; D015415:Biomarkers / Q000097:blood; D015992:Body Mass Index; D002097:C-Reactive Protein / Q000032:analysis; D018932:Chemokine CCL2 / Q000097:blood*; D005260:Female; D005307:Fertilization in Vitro / Q000009:adverse effects; D015571:Follicular Fluid / Q000378:metabolism*; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood; D006801:Humans; D007247:Infertility, Female / Q000097:blood / Q000175:diagnosis / Q000503:physiopathology / Q000628:therapy*; D009765:Obesity / Q000097:blood* / Q000175:diagnosis / Q000503:physiopathology; D065851:Ovarian Reserve; D010865:Pilot Projects; D011247:Pregnancy; D018873:Pregnancy Rate; D012372:ROC Curve; D012307:Risk Factors; D016896:Treatment Outcome; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; C111835:CCL2 protein, human; D018932:Chemokine CCL2; D016179:Granulocyte Colony-Stimulating Factor; D002097:C-Reactive Protein
keywords: IVF; MCP-1; Obesity; follicular fluid; ovary