title: Expression of apical junction complex proteins in colorectal mucosa of miniature dachshunds with inflammatory colorectal polyps.
abstract: We examine the expression of tight junction and adherence junction proteins in the colorectal mucosa of miniature dachshunds (MDs) with inflammatory colorectal polyps (ICRPs). Colorectal mucosa samples were endoscopically obtained from 8 MDs with ICRPs and 8 control dogs for immunoblotting. Paraffin-embedded tissues of surgically resected inflamed lesions from another 5 MDs with ICRPs and full-thickness colorectal specimens from 5 healthy beagles were obtained for immunohistochemistry. The expression patterns of claudin-1, -2, -3, -4, -5, -7 and -8, E-cadherin and β-catenin were analyzed in the non-inflamed mucosa and inflamed mucosa of ICRPs and colorectal mucosa of control dogs by immunoblotting. The localization of these proteins in the inflamed lesions was analyzed by immunohistochemistry. The expressions of each of claudin, E-cadherin and β-catenin were not significantly different between control dogs and non-inflamed colonic mucosa from MDs with ICRPs. In contrast, only E-cadherin and β-catenin were detected in the inflamed lesions of MDs with ICRPs. By immunohistochemistry, claudin-2, -3, -4, -5 and -7, E-cadherin and β-catenin were expressed in the colorectal epithelium within the inflamed mucosa, but not in granulation tissue. Distributions of claudin-2, -3, -4, -5, and -7, E-cadherin and β-catenin in the colonic epithelium were not different between MDs with ICRPs and control dogs. These results indicated that no significant alteration was detected in several tight junction or adherence junction proteins expression in the colorectal epithelium of ICRPs.
authors.affiliation: Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan.
authors.forename: Nozomu
authors.initials: N
authors.lastname: Yokoyama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ohta
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Kagawa
authors.forename: Rommaneeya
authors.initials: R
authors.lastname: Leela-Arporn
authors.forename: Angkhana
authors.initials: A
authors.lastname: Dermlim
authors.forename: Khoirun
authors.initials: K
authors.lastname: Nisa
authors.forename: Tomoya
authors.initials: T
authors.lastname: Morita
authors.forename: Tatsuyuki
authors.initials: T
authors.lastname: Osuga
authors.forename: Noboru
authors.initials: N
authors.lastname: Sasaki
authors.forename: Keitaro
authors.initials: K
authors.lastname: Morishita
authors.forename: Kensuke
authors.initials: K
authors.lastname: Nakamura
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Takiguchi
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism; D057167:Claudins / Q000378:metabolism; D003111:Colonic Polyps / Q000378:metabolism / Q000662:veterinary; D017630:Connexins / Q000378:metabolism; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D005260:Female; D007413:Intestinal Mucosa / Q000378:metabolism*; D007417:Intestinal Polyps / Q000378:metabolism / Q000662:veterinary*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D019108:Tight Junctions / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
chemical_list: D015820:Cadherins; D057167:Claudins; D017630:Connexins; D008565:Membrane Proteins; D051176:beta Catenin