title: Upregulated lncRNA-PCAT1 is closely related to clinical diagnosis of multiple myeloma as a predictive biomarker in serum.
abstract: OBJECTIVE
  The purpose of this study was to explore serum PCAT-1 expression in multiple myeloma (MM) and examine the potential usefulness of this molecule as a biomarker for diagnosis in MM.
  
  
  METHODS
  Serum samples were collected from 60 newly diagnosed untreated MM patients, and 48 healthy controls. Serum PCAT-1 expression levels were detected by RT-qPCR. In addition, correlations between the relative expression of serum PCAT-1 and the concentrations of lactic acid dehydrogenase (LDH), β2M, λ light chain and κ light chain were assessed.
  
  
  RESULTS
  It was found that the relative expression of serum PCAT-1 in MM patients (81.02 ± 136.9) was higher than that in healthy controls (3.17 ± 5.75) (U= 307.0, P< 0.0001) and was significantly correlated with β2M concentration (r= 0.461, P= 0.0002), but not with LDH, κ light and λ light chain concentration (r= 0.061, P= 0.641; r= 0.007, P= 0.956; r=-0.090, P= 0.499 respectively). Additionally, it was significantly correlated with different isotype of MM (H= 7.464, P= 0.024). The AUC of the ROC curve of serum PCAT-1 was 0.892 (95% CI 0.833-0.950), which was higher than other markers. Combining PCAT-1 and β2M together, the sensitivity was highest compared with other markers alone, or combined.
  
  
  CONCLUSIONS
  The expression levels of serum PCAT-1 in MM patients were significantly higher than that in healthy controls, suggesting that it may be useful in the auxiliary diagnosis of MM.
authors.affiliation: Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China.
authors.forename: Xianjuan
authors.initials: X
authors.lastname: Shen
authors.affiliation: Laboratory Medicine Center, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Yancheng Central Blood Station, Yancheng 224000, Jiangsu, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Wu
authors.affiliation: Nantong University, Nantong 226001, Jiangsu, China.
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.affiliation: Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Qi
authors.affiliation: Laboratory Medicine Center, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Cong
authors.affiliation: Laboratory Medicine Center, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Wu
authors.affiliation: Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China.|Laboratory Medicine Center, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China.
authors.forename: Shaoqing
authors.initials: S
authors.lastname: Ju
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers; D014408:Biomarkers, Tumor; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000175:diagnosis* / Q000235:genetics*; D009367:Neoplasm Staging; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000097:blood / Q000235:genetics*; D012372:ROC Curve; D015203:Reproducibility of Results
chemical_list: D015415:Biomarkers; D014408:Biomarkers, Tumor; C588212:PCAT-1 lncRNA, human; D062085:RNA, Long Noncoding
keywords: Multiple myeloma; PCAT-1; auxiliary diagnosis; real-time quantitative PCR