title: Electrochemiluminescence Detection of Escherichia coli O157:H7 Based on a Novel Polydopamine Surface Imprinted Polymer Biosensor.
abstract: In this paper, a facilely prepared electrochemiluminescence (ECL) biosensor was developed for Escherichia coli O157:H7 quantitative detection based on a polydopamine (PDA) surface imprinted polymer (SIP) and nitrogen-doped graphene quantum dots (N-GQDs). N-GQDs with a high quantum yield of 43.2% were synthesized. The uniform PDA SIP film for E. coli O157:H7 was established successfully with a facile route. The dopamine and target bacteria were electropolymerized directly on the electrode. After removal of the E. coli O157:H7 template, the established PDA SIP can selectively recognize E. coli O157:H7. Accordingly, E. coli O157:H7 polyclonal antibody (pAb) was labeled with N-GQDs. The bioconjugation of SIP-E. coli O157:H7/pAb-N-GQDs can generate intensive ECL irradiation with K2S2O8. As a result, E. coli O157:H7 was detected with the ECL sensing system. Under optimal conditions, the linear relationships between the ECL intensity and E. coli O157:H7 concentration were obtained from 101colony-forming units (CFU) mL-1to 107CFU mL-1with a limit of detection of 8 CFU mL-1. The biosensor based on this SIP film was applied in water sample detection successfully. The N-GQD-based ECL analytical method for E. coli O157:H7 was reported for the first time. The sensing system had high selectivity to the target analyte, provided new opportunities for use, and increased the rate of disease diagnosis and treatment and the prevention of pathogens.
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University , Changchun 130012, China.
authors.forename: Shufan
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University , Changchun 130012, China.
authors.forename: Xueqian
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory for Supramolecular Structure and Materials, College of Chemistry, Jilin University , Changchun 130012, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University , Changchun 130012, China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University , Changchun 130012, China.
authors.forename: Qiang
authors.identifier: http://orcid.org/0000-0003-4841-5005
authors.initials: Q
authors.lastname: Ma
mesh_terms: D015374:Biosensing Techniques; D019453:Escherichia coli O157; D007211:Indoles; D054802:Molecular Imprinting; D011108:Polymers
chemical_list: D007211:Indoles; D011108:Polymers; C568283:polydopamine
keywords: E. coli O157:H7; dopamine; electrochemiluminescence; electropolymerization; nitrogen-doped graphene quantum dot; surface imprinted polymer