title: Cell Selective Apoptosis Induced by Polymorphic Alteration of Self-Assembled Silica Nanowebs.
abstract: The biocompatibility of silicon-based nanomaterials makes them suitable for biophysical and biomedical applications. However, the application of silicon-based nanomaterials has been mainly restricted to nanoparticles (NPs) as a potential drug carrier and the extracellular matrix (ECM) as a platform for cell adhesion and proliferation. Here, we introduce silica NPs self-assembled into a 3D nanoweb architecture that was shown to inherit the therapeutic and proliferative attributes of both NPs and ECMs. The self-assembled silica nanoweb (SNW) has, therefore, not only shown targeted druglike behavior in HeLa cells without the use of biomarkers but has also shown ECM characteristics. The ECM characteristics of SNWs enhanced the cellular attraction and proliferation by which fibroblasts exhibited tissuelike behavior, and HeLa cells underwent an intensified induction of apoptosis. These properties are tailored by the alteration of the polymorphic heterogeneities of the SNW as a novel nanobiointerface for exceptional apoptosis induction through the enhancement of cellular attraction, which, to the best of our knowledge, has not been previously reported. These attributes enable selective functionality with which cancerous HeLa and mammalian fibroblast cells were affected differently. Moreover, simultaneous control of the packing index and crystallinity of the SNWs, to which the cells had been attracted, possessed the additional advantage of modulating the selective functionality of this nanobiointerface. These polymorphic characteristics were tailored by the alteration of the crystallinity of the synthesized SNW via precision control of the ionization level of the silicon substrate, whose requisite ionization energy was generated by an ultrashort pulsed laser. Our results showed that the therapeutic functionality of the SNW-plated template can be elucidated via the endocytosis of amorphous SNWs. Because of the efficient cellular attraction and remarkable contrast in the cellular response to the SNW-plated template, we expect that these findings will provide new insights and opportunities for designing and engineering novel cell-material interfaces for advanced biomedical applications in cancer research.
authors.affiliation: Micro/Nanofabrication Laboratory, Department of Aerospace Engineering, Ryerson University , 350 Victoria Street, Toronto, Ontario M5B 2K3, Canada.|Institute for Biomedical Engineering, Science and Technology (iBEST), Partnership between Ryerson University and St. Michael's Hospital , Toronto, Ontario M5B 1W8, Canada.
authors.forename: Meysam
authors.identifier: http://orcid.org/0000-0002-6813-4860
authors.initials: M
authors.lastname: Keshavarz
authors.affiliation: Micro/Nanofabrication Laboratory, Department of Aerospace Engineering, Ryerson University , 350 Victoria Street, Toronto, Ontario M5B 2K3, Canada.
authors.forename: Bo
authors.initials: B
authors.lastname: Tan
authors.affiliation: Ultrashort Laser Nanomanufacturing Research Facility, Department of Mechanical and Industrial Engineering, Ryerson University , 350 Victoria Street, Toronto, ON M5B 2K3, Canada.|Keenan Research Centre for Biomedical Science, St. Michael's Hospital , Toronto, Ontario M5B 1W8, Canada.
authors.forename: Krishnan
authors.identifier: http://orcid.org/0000-0002-7363-8588
authors.initials: K
authors.lastname: Venkatakrishnan
keywords: HeLa; cell selectivity; crystallinity changes; endocytosis; fibroblasts; self-assembled silica nanoweb