title: Antibiotics-induced depletion of mice microbiota induces changes in host serotonin biosynthesis and intestinal motility.
abstract: The gastrointestinal motility is affected by gut microbiota and the relationship between them has become a hot topic. However, mechanisms of microbiota in regulating motility have not been well defined. We thus investigated the effect of microbiota depletion by antibiotics on gastrointestinal motility, colonic serotonin levels, and bile acids metabolism.
  
  
  
  After 4 weeks with antibiotics treatments, gastrointestinal and colon transit, defecation frequency, water content, and other fecal parameters were measured and analyzed in both wild-type and antibiotics-treated mice, respectively. Contractility of smooth muscle, serotonin levels, and bile acids levels in wild-type and antibiotics-treated mice were also analyzed.
  
  
  
  After antibiotics treatment, the richness and diversity of intestinal microbiota decreased significantly, and the fecal of mice had less output (P < 0.01), more water content (P < 0.01), and longer pellet length (P < 0.01). Antibiotics treatment in mice also resulted in delayed gastrointestinal and colonic motility (P < 0.05), and inhibition of phasic contractions of longitudinal muscle from isolated proximal colon (P < 0.01). In antibiotics-treated mice, serotonin, tryptophan hydroxylase 1, and secondary bile acids levels were decreased.
  
  
  
  Gut microbiota play an important role in the regulation of intestinal bile acids and serotonin metabolism, which could probably contribute to the association between gut microbiota and gastrointestinal motility as intermediates.
authors.affiliation: Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, 305 East Zhongshan Road, 210002, Nanjing, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Ge
authors.affiliation: Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, 305 East Zhongshan Road, 210002, Nanjing, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Ding
authors.affiliation: Model Animal Research Center and MOE Key Laboratory of Model Animal for Disease Study, Nanjing University, Nanjing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Medical Genetics, and Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, Nanjing, China.
authors.forename: Lizhi
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, 305 East Zhongshan Road, 210002, Nanjing, China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Tian
authors.affiliation: Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, 305 East Zhongshan Road, 210002, Nanjing, China. jinlingh_gongjf@163.com.
authors.forename: Jianfeng
authors.identifier: 0000-0002-9833-1806
authors.initials: J
authors.lastname: Gong
authors.affiliation: Model Animal Research Center and MOE Key Laboratory of Model Animal for Disease Study, Nanjing University, Nanjing, China.
authors.forename: Minsheng
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, 305 East Zhongshan Road, 210002, Nanjing, China.
authors.forename: Jieshou
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, 305 East Zhongshan Road, 210002, Nanjing, China. jinlingh_lining@163.com.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001647:Bile Acids and Salts / Q000378:metabolism; D002432:Cecum / Q000187:drug effects / Q000473:pathology; D005243:Feces; D000069196:Gastrointestinal Microbiome / Q000187:drug effects; D005769:Gastrointestinal Motility / Q000187:drug effects*; D008297:Male; D055442:Metabolome / Q000187:drug effects; D008810:Mice, Inbred C57BL; D064307:Microbiota / Q000187:drug effects*; D009119:Muscle Contraction; D009929:Organ Size / Q000187:drug effects; D012701:Serotonin / Q000096:biosynthesis*
chemical_list: D000900:Anti-Bacterial Agents; D001647:Bile Acids and Salts; D012701:Serotonin
keywords: Antibiotics; Bile acids; Gut motility; Intestinal microbiota; Serotonin