title: [Effect and its molecular mechanisms of curcumin on pulmonary artery smooth muscle cells in rat model with chronic obstructive pulmonary disease].
abstract: Objective:To investigate the effects and the underlying molecular mechanisms of curcumin on pulmonary artery smooth muscle cells in rat model with chronic obstructive pulmonary disease (COPD).Methods:A total of 75 male Wistar rats were randomly divided into control group (group CN), model group (group M), low-dose curcumin group (group CL), medium-dose curcumin group (group CM) and high-dose curcumin group (group CH). HE staining was used to observe the morphology of pulmonary artery. Proliferating cell nuclear antigen (PCNA), apoptosis-related protein Bcl-2 and Bax were detected by immunohistochemical staining. TUNEL kit was used to analyze the effects of curcumin on apoptosis of smooth muscle cells, and the protein expressions of SOCS-3/JAK2/STAT pathway in lung tissues were determined by western blot.Results:Right ventricular systolic pressure (RVSP) and right ventricular hypertrophy index (RVMI) in group M were significantly higher than those in group CN, group CH and group CM (allP<0.05). HE staining and TUNEL kit test showed that the number of pulmonary artery smooth muscle cells had a significant increase in group M, while the pulmonary artery tube became thin, and the smooth muscle cells shrinked in group CM and group CH. Immunohistochemistry showed that PCNA and Bcl-2 in group M were significantly higher than those in group CN (allP<0.05), while Bax expression was significantly lower than that in group CN (P<0.05). PCNA in group CM and group CH were significantly lower than that in group M (allP<0.05), while Bax expression was significantly higher than that in group M (P<0.05). Western blot showed that SOCS-3 protein was significantly decreased in group M, while the p-JAK2, p-STAT1, p-STAT3 were significantly increased (allP<0.05). Compared with group M, SOCS-3 protein in group CM and group CH were significantly increased (allP<0.05), while the p-JAK2, p-STAT3 were significantly reduced (allP<0.05).Conclusion:Curcumin could promote the apoptosis of smooth muscle cells in rats with COPD, and improve the mean pulmonary artery pressure and RVMI through stimulating SOCS-3/JAK2/STAT signaling pathway.
authors.affiliation: Acupuncture Clinical Traumatology Institute, Anhui University of Chinese Medicine, Hefei 230031, China.
authors.forename: Xiangang
authors.initials: X
authors.lastname: Lin
authors.affiliation: Acupuncture Clinical Traumatology Institute, Anhui University of Chinese Medicine, Hefei 230031, China.
authors.forename: Yenong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Acupuncture Clinical Traumatology Institute, Anhui University of Chinese Medicine, Hefei 230031, China.
authors.forename: Zhuqing
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D062186:Arterial Pressure / Q000187:drug effects* / Q000502:physiology; D003474:Curcumin / Q000494:pharmacology*; D017380:Hypertrophy, Right Ventricular / Q000473:pathology / Q000503:physiopathology; D053614:Janus Kinase 2 / Q000187:drug effects / Q000502:physiology; D008168:Lung / Q000737:chemistry / Q000187:drug effects; D008297:Male; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000473:pathology*; D018809:Proliferating Cell Nuclear Antigen / Q000187:drug effects / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000187:drug effects / Q000378:metabolism; D011651:Pulmonary Artery / Q000187:drug effects* / Q000473:pathology*; D029424:Pulmonary Disease, Chronic Obstructive / Q000473:pathology* / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D050791:STAT Transcription Factors; D000071223:Suppressor of Cytokine Signaling 3 Protein / Q000187:drug effects / Q000502:physiology; D017725:Ventricular Pressure / Q000187:drug effects; D051028:bcl-2-Associated X Protein / Q000187:drug effects / Q000378:metabolism
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; D050791:STAT Transcription Factors; C491410:Socs3 protein, rat; D000071223:Suppressor of Cytokine Signaling 3 Protein; D051028:bcl-2-Associated X Protein; C507924:JAK2 protein, human; D053614:Janus Kinase 2; D003474:Curcumin