title: Mycelium transformation of Streptomyces toxytricini into pellet: Role of culture conditions and kinetics.
abstract: The present study envisages the role of different carbon sources, nitrogen sources, metals, pH, inoculum volume and agitation rate in pellet formation of S. toxytricini at shake-flask level. It was found that galactose, ammonium sulphate, sodium nitrate, Cu2+, Zn2+, higher inoculum volume (5% v/v) and agitation rate at 300rpm caused significant reduction in pellet size (up to the range of 30μm-0.5mm) but biomass formations was also reduced subsequently. Interestingly diffused type of morphology was obtained in Fe2+supplemented medium with reduced biomass (1.5gL-1). Rheological study revealed that non-Newtonian behaviour of culture broth. Besides this, kinetics study was also made to understand the growth kinetics (0.39gL-1h-1), oxygen uptake rate (0.1146mgL-1h-1), and production of lipstatin (0.0072gh-1).
authors.affiliation: Microbial Biotechnology Laboratory, University Institute of Engineering and Technology, Maharshi Dayanand University, Rohtak 124001, Haryana, India.
authors.forename: Punit
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Microbial Biotechnology Laboratory, University Institute of Engineering and Technology, Maharshi Dayanand University, Rohtak 124001, Haryana, India; Department of Biotechnology, Central University of Haryana, Mahendergarh 123031, Haryana, India. Electronic address: kashyapdubey@gmail.com.
authors.forename: Kashyap Kumar
authors.initials: KK
authors.lastname: Dubey
mesh_terms: D001681:Biological Assay; D018533:Biomass; D002244:Carbon / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods*; D004058:Diffusion; D005285:Fermentation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007783:Lactones / Q000378:metabolism; D008962:Models, Theoretical; D025282:Mycelium / Q000187:drug effects / Q000378:metabolism*; D009584:Nitrogen / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism; D012212:Rheology / Q000187:drug effects; D012492:Salts / Q000494:pharmacology; D013302:Streptomyces / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
chemical_list: D007783:Lactones; D012492:Salts; D002244:Carbon; C054164:lipstatin; D009584:Nitrogen; D010100:Oxygen
keywords: Diffusivity; Fermentation; Lipstatin; Mass transfer; Obesity; Rheology