title: Identification of Serratane Synthase Gene from the Fern Lycopodium clavatum.
abstract: Ferns are known to produce onoceroids including onoceranes and serratanes having unusual structures among triterpenes. From the fern Lycopodium clavatum, a novel onoceroid synthase gene was cloned that showed high sequence identity with a previously identified α-onocerin synthase. Functional analysis by coexpression with pre-α-onocerin synthase in yeast led to the production of tohogenol and serratenediol. The result suggested that serratanes are directly biosynthesized from pre-α-onocerin and not from α-onocerin as previously assumed.
authors.affiliation: Graduate School of Agriculture, Meiji University , 1-1-1 Higashimita, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Saga
authors.affiliation: Graduate School of Agriculture, Meiji University , 1-1-1 Higashimita, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Araki
authors.affiliation: Graduate School of Agriculture, Meiji University , 1-1-1 Higashimita, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Araya
authors.affiliation: Graduate School of Pharmaceutical Sciences, Chiba University , 1-8-1 Chuo-ku, Chiba 260-8675, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Saito
authors.affiliation: Graduate School of Pharmaceutical Sciences, Chiba University , 1-8-1 Chuo-ku, Chiba 260-8675, Japan.
authors.forename: Mami
authors.initials: M
authors.lastname: Yamazaki
authors.affiliation: Kazusa DNA Research Institute , 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Suzuki
authors.affiliation: Graduate School of Agriculture, Meiji University , 1-1-1 Higashimita, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan.
authors.forename: Tetsuo
authors.identifier: 0000-0002-7598-4791
authors.initials: T
authors.lastname: Kushiro
mesh_terms: D029624:Ferns; D032502:Lycopodium / Q000737:chemistry*; D015394:Molecular Structure