title: Variations of Polymer Porous Surface Structures via the Time-Sequenced Dosing of Mixed Solvents.
abstract: A new approach to polystyrene surface treatment via the time-sequenced dispensing of good and poor solvent mixtures on the rotating surface of treated substrate is presented in this study. It is demonstrated that the variation of the sequencing together with other variables (e.g., temperature and solvent concentration) affects the size and depth of pores evolving on the polystyrene surface. A model of the surface pore creation, associated with the viscoelastic phase separation, surface tension, and secondary flows caused by temperature variations and the rapid evaporation of the good solvent is proposed. Experimental results of profilometric, goniometric, and optical measurements show that this approach enables the simple and quick preparation of surfaces with various numbers, diameters, and depths of individual pores, which ultimately affects not only the wetting characteristics of the surfaces but also the fate of cells cultivated there. The presented method allows the easy preparation of a large number of structured substrates for effective cell cultivation and proliferation.
authors.affiliation: Department of Physics and Materials Engineering, Tomas Bata University in Zlín , Vavrečkova 275, 760 01 Zlín, Czech Republic.|Centre of Polymer Systems, Tomas Bata University in Zlín , Třída Tomáše Bati 5678, 76001 Zlín, Czech Republic.
authors.forename: Erik
authors.initials: E
authors.lastname: Wrzecionko
authors.affiliation: Department of Physics and Materials Engineering, Tomas Bata University in Zlín , Vavrečkova 275, 760 01 Zlín, Czech Republic.|Centre of Polymer Systems, Tomas Bata University in Zlín , Třída Tomáše Bati 5678, 76001 Zlín, Czech Republic.
authors.forename: Antonín
authors.identifier: http://orcid.org/0000-0002-0055-675X
authors.initials: A
authors.lastname: Minařík
authors.affiliation: Department of Physics and Materials Engineering, Tomas Bata University in Zlín , Vavrečkova 275, 760 01 Zlín, Czech Republic.|Centre of Polymer Systems, Tomas Bata University in Zlín , Třída Tomáše Bati 5678, 76001 Zlín, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Smolka
authors.affiliation: Department of Physics and Materials Engineering, Tomas Bata University in Zlín , Vavrečkova 275, 760 01 Zlín, Czech Republic.|Centre of Polymer Systems, Tomas Bata University in Zlín , Třída Tomáše Bati 5678, 76001 Zlín, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Minařík
authors.affiliation: Centre of Polymer Systems, Tomas Bata University in Zlín , Třída Tomáše Bati 5678, 76001 Zlín, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Humpolíček
authors.affiliation: Centre of Polymer Systems, Tomas Bata University in Zlín , Třída Tomáše Bati 5678, 76001 Zlín, Czech Republic.
authors.forename: Petra
authors.initials: P
authors.lastname: Rejmontová
authors.affiliation: Department of Physics and Materials Engineering, Tomas Bata University in Zlín , Vavrečkova 275, 760 01 Zlín, Czech Republic.|Centre of Polymer Systems, Tomas Bata University in Zlín , Třída Tomáše Bati 5678, 76001 Zlín, Czech Republic.
authors.forename: Aleš
authors.initials: A
authors.lastname: Mráček
authors.affiliation: Department of Physics and Materials Engineering, Tomas Bata University in Zlín , Vavrečkova 275, 760 01 Zlín, Czech Republic.|Centre of Polymer Systems, Tomas Bata University in Zlín , Třída Tomáše Bati 5678, 76001 Zlín, Czech Republic.
authors.forename: Magda
authors.initials: M
authors.lastname: Minaříková
authors.affiliation: Centre of Polymer Systems, Tomas Bata University in Zlín , Třída Tomáše Bati 5678, 76001 Zlín, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Gřundělová
keywords: cells; phase separation; polystyrene; pores; solvent mixture; surface; surface tension; wetting