title: Quantification of hepcidin-25 in human cerebrospinal fluid using LC-MS/MS.
abstract: OBJECTIVE
  Hepcidin, the main iron metabolism regulator, can be detected in various biological fluids. Here, we describe a quantitative method of LC-MS/MS to quantify the 25 amino acid isoform of hepcidin (hepcidin-25) in human cerebrospinal fluid (CSF). Results & methodology: Samples were prepared through protein precipitation followed by solid phase extraction (SPE) and injected into a triple-quadrupole mass spectrometer. Validation of our method included determination of LOQ (0.1 ng/ml), repeatability, intermediate precision, recovery and linearity (up to 25 ng/ml). Hepcidin-25 was subsequently quantified in 36 human CSF samples and its concentration ranged from 0.21 to 3.54 ng/ml.
  
  
  CONCLUSIONS
  This is the first time that hepcidin-25 can be reliably quantified in human CSF. This may open interesting perspectives for the management of iron-related neurological disorders.
authors.affiliation: Laboratoire de Biochimie et de Protéomique Clinique - Institut de Médecine Régénérative et Biothérapies (LBPC-IRMB), CHU de Montpellier, 34250 Montpellier, France.
authors.forename: Constance
authors.initials: C
authors.lastname: Delaby
authors.affiliation: Laboratoire de Biochimie et de Protéomique Clinique - Institut de Médecine Régénérative et Biothérapies (LBPC-IRMB), CHU de Montpellier, 34250 Montpellier, France.|Laboratoire National de Métrologie et d'Essais (LNE), 75015 Paris, France.
authors.forename: Pauline
authors.initials: P
authors.lastname: Bros
authors.affiliation: Laboratoire de Biochimie et de Protéomique Clinique - Institut de Médecine Régénérative et Biothérapies (LBPC-IRMB), CHU de Montpellier, 34250 Montpellier, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Vialaret
authors.affiliation: Laboratoire de Biochimie et de Protéomique Clinique - Institut de Médecine Régénérative et Biothérapies (LBPC-IRMB), CHU de Montpellier, 34250 Montpellier, France.
authors.forename: Amandine
authors.initials: A
authors.lastname: Moulinier
authors.affiliation: Laboratoire National de Métrologie et d'Essais (LNE), 75015 Paris, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Delatour
authors.affiliation: Laboratoire de Biochimie et de Protéomique Clinique - Institut de Médecine Régénérative et Biothérapies (LBPC-IRMB), CHU de Montpellier, 34250 Montpellier, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Gabelle
authors.affiliation: Laboratoire de Biochimie et de Protéomique Clinique - Institut de Médecine Régénérative et Biothérapies (LBPC-IRMB), CHU de Montpellier, 34250 Montpellier, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Lehmann
authors.affiliation: Laboratoire de Biochimie et de Protéomique Clinique - Institut de Médecine Régénérative et Biothérapies (LBPC-IRMB), CHU de Montpellier, 34250 Montpellier, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Hirtz
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D006041:Goats; D064451:Hepcidins / Q000097:blood / Q000134:cerebrospinal fluid*; D006801:Humans; D007501:Iron / Q000737:chemistry; D057230:Limit of Detection; D016014:Linear Models; D009422:Nervous System Diseases / Q000134:cerebrospinal fluid / Q000175:diagnosis; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry; D000511:alpha-Macroglobulins / Q000134:cerebrospinal fluid
chemical_list: D064451:Hepcidins; D000511:alpha-Macroglobulins; C545475:hepcidin 25, human; D007501:Iron
keywords: LC–MS/MS; MS; cerebrospinal fluid; hepcidin-25; neurological disorders