title: miR-29b promotes skin wound healing and reduces excessive scar formation by inhibition of the TGF-β1/Smad/CTGF signaling pathway.
abstract: The hypertrophic scar is a medical difficulty of humans, which has caused great pain to patients. Here, we investigated the inhibitory effect of miR-29b on scar formation. The scalded model was established in mice and miR-29b mimics or a negative control was subcutaneously injected into the injury skin. Then various molecular biological experiments were performed to assess the effect of miR-29b on scar formation. According to our present study, first, the results demonstrated that miR-29b was down-regulated in thermal injury tissue and miR-29b treatment could promote wound healing, inhibit scar formation, and alleviate histopathological morphologic alteration in scald tissues. Additionally, miR-29b treatment suppressed collagen deposition and fibrotic gene expression in scar tissues. Finally, we found that miR-29b treatment inhibited the TGF-β1/Smad/CTGF signaling pathway. Taken together, our data suggest that miR-29b treatment has an inhibitory effect against scar formation via inhibition of the TGF-β1/Smad/CTGF signaling pathway and may provide a potential molecular basis for future treatments for hypertrophic scars.
authors.affiliation: Department of Plastic and Reconstructive Surgery, The First Hospital of Jilin University, 71 Xinmin Avenue, Changchun 130021, People's Republic of China.|Department of Plastic and Reconstructive Surgery, The First Hospital of Jilin University, 71 Xinmin Avenue, Changchun 130021, People's Republic of China.
authors.forename: Jingdong
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Plastic and Reconstructive Surgery, The First Hospital of Jilin University, 71 Xinmin Avenue, Changchun 130021, People's Republic of China.|Department of Plastic and Reconstructive Surgery, The First Hospital of Jilin University, 71 Xinmin Avenue, Changchun 130021, People's Republic of China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Lin
authors.affiliation: Department of Plastic and Reconstructive Surgery, The First Hospital of Jilin University, 71 Xinmin Avenue, Changchun 130021, People's Republic of China.|Department of Plastic and Reconstructive Surgery, The First Hospital of Jilin University, 71 Xinmin Avenue, Changchun 130021, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Shao
authors.affiliation: Department of Plastic and Reconstructive Surgery, The First Hospital of Jilin University, 71 Xinmin Avenue, Changchun 130021, People's Republic of China.|Department of Plastic and Reconstructive Surgery, The First Hospital of Jilin University, 71 Xinmin Avenue, Changchun 130021, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Rong
authors.affiliation: Department of Plastic and Reconstructive Surgery, The First Hospital of Jilin University, 71 Xinmin Avenue, Changchun 130021, People's Republic of China.|Department of Plastic and Reconstructive Surgery, The First Hospital of Jilin University, 71 Xinmin Avenue, Changchun 130021, People's Republic of China.
authors.forename: Duo
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017439:Cicatrix, Hypertrophic / Q000378:metabolism / Q000628:therapy*; D003094:Collagen / Q000378:metabolism*; D055513:Connective Tissue Growth Factor / Q000378:metabolism; D004195:Disease Models, Animal; D015536:Down-Regulation; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000378:metabolism / Q000627:therapeutic use*; D058990:Molecular Targeted Therapy; D015398:Signal Transduction; D051896:Smad Proteins, Receptor-Regulated / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014945:Wound Healing
chemical_list: C526318:CCN2 protein, mouse; C547014:MIRN29 microRNA, mouse; D035683:MicroRNAs; D051896:Smad Proteins, Receptor-Regulated; C508961:Tgfb1 protein, mouse; D053773:Transforming Growth Factor beta1; D055513:Connective Tissue Growth Factor; D003094:Collagen
keywords: CTGF; Smad2/3; TGF-β1; formation de cicatrices; miR-29b; scar formation