title: Dissociation Between Brown Adipose Tissue18F-FDG Uptake and Thermogenesis in Uncoupling Protein 1-Deficient Mice.
abstract: 18F-FDG PET imaging is routinely used to investigate brown adipose tissue (BAT) thermogenesis, which requires mitochondrial uncoupling protein 1 (UCP1). It remains uncertain, however, whether BAT18F-FDG uptake is a reliable surrogate measure of UCP1-mediated heat production.Methods:UCP1 knockout (KO) and wild-type (WT) mice housed at thermoneutrality were treated with the selective β3 adrenergic receptor agonist CL 316, 243 and underwent metabolic cage, infrared thermal imaging and18F-FDG PET/MRI experiments. Primary brown adipocytes were additionally examined for their bioenergetics by extracellular flux analysis as well as their uptake of 2-deoxy-3H-glucose.Results:In response to CL 316, 243 treatments, oxygen consumption, and BAT thermogenesis were diminished in UCP1 KO mice, but BAT18F-FDG uptake was fully retained. Isolated UCP1 KO brown adipocytes exhibited defective induction of uncoupled respiration whereas their glycolytic flux and 2-deoxy-3H-glucose uptake rates were largely unaffected.Conclusion:Adrenergic stimulation can increase BAT18F-FDG uptake independently of UCP1 thermogenic function.
authors.affiliation: Integrated Research and Treatment Centre for Adiposity Diseases, Department of Medicine, University of Leipzig, Leipzig, Germany.
authors.forename: Mohammed K
authors.initials: MK
authors.lastname: Hankir
authors.affiliation: Institute of Radiopharmaceutical Cancer Research, Department of Neuroradiopharmaceuticals, Helmholtz Centre Dresden-Rossendorf, Leipzig, Germany.
authors.forename: Mathias
authors.initials: M
authors.lastname: Kranz
authors.affiliation: Helmholtz Diabetes Center, Helmholtz Center Munich, Neuherburg, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Keipert
authors.affiliation: Collaborative Research Center for Obesity Mechanisms, University of Leipzig, Leipzig, Germany; and.
authors.forename: Juliane
authors.initials: J
authors.lastname: Weiner
authors.affiliation: Integrated Research and Treatment Centre for Adiposity Diseases, Department of Medicine, University of Leipzig, Leipzig, Germany.
authors.forename: Sille G
authors.initials: SG
authors.lastname: Andreasen
authors.affiliation: Integrated Research and Treatment Centre for Adiposity Diseases, Department of Medicine, University of Leipzig, Leipzig, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Kern
authors.affiliation: Department of Nuclear Medicine, Leipzig University Hospital, Leipzig, Germany.
authors.forename: Marianne
authors.initials: M
authors.lastname: Patt
authors.affiliation: Integrated Research and Treatment Centre for Adiposity Diseases, Department of Medicine, University of Leipzig, Leipzig, Germany.
authors.forename: Nora
authors.initials: N
authors.lastname: Klöting
authors.affiliation: Collaborative Research Center for Obesity Mechanisms, University of Leipzig, Leipzig, Germany; and.
authors.forename: John T
authors.initials: JT
authors.lastname: Heiker
authors.affiliation: Institute of Radiopharmaceutical Cancer Research, Department of Neuroradiopharmaceuticals, Helmholtz Centre Dresden-Rossendorf, Leipzig, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Brust
authors.affiliation: Integrated Research and Treatment Centre for Adiposity Diseases, Department of Medicine, University of Leipzig, Leipzig, Germany.|Department of Nuclear Medicine, Leipzig University Hospital, Leipzig, Germany.
authors.forename: Swen
authors.initials: S
authors.lastname: Hesse
authors.affiliation: Helmholtz Diabetes Center, Helmholtz Center Munich, Neuherburg, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Jastroch
authors.affiliation: Integrated Research and Treatment Centre for Adiposity Diseases, Department of Medicine, University of Leipzig, Leipzig, Germany WiebkeKristin.Fenske@medizin.uni-leipzig.de.
authors.forename: Wiebke K
authors.initials: WK
authors.lastname: Fenske
mesh_terms: D002001:Adipose Tissue, Brown / Q000000981:diagnostic imaging* / Q000378:metabolism*; D000818:Animals; D005260:Female; D019788:Fluorodeoxyglucose F18 / Q000493:pharmacokinetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D049268:Positron-Emission Tomography / Q000379:methods*; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D022722:Thermogenesis / Q000502:physiology*; D000071256:Uncoupling Protein 1 / Q000378:metabolism*
chemical_list: D019275:Radiopharmaceuticals; C000604372:Ucp1 protein, mouse; D000071256:Uncoupling Protein 1; D019788:Fluorodeoxyglucose F18
keywords: PET/MRI; animal imaging; brown adipose tissue; glucose metabolism; neuroendocrine; thermogenesis; uncoupling protein 1