title: B7-H3 participates in the development of Asthma by augmentation of the inflammatory response independent of TLR2 pathway.
abstract: B7-H3, a new member of the B7 superfamily, acts as both a T cell costimulator and coinhibitor. Recent studies identified B7-H3 plays a critical role in the development of asthma. But the definitive mechanism is not clear. In this study, we further report that B7-H3 participates in the development of OVA-induced asthma in a murine model. And study its mechanism through the vitro and vivo experiment. Exogenous administration of B7-H3 strongly amplified the inflammatory response and augmented proinflammatory cytokines in vitro and vivo. These B7-H3-associated proinflammatory effects were not dependent on TLR2 signaling, as airway inflammation, eosinophils infiltration and cytokins (IL-4, IL-5, IL-13 and IFN-gamma) augment were still amplified in TLR2-deficient mice after administrated recombinant mouse B7-H3. These results indicated an important role for B7-H3 in the development of Th1 and Th2 cells in a murine model of asthma and its proinflammatory effects are not dependent on TLR2 signaling.
authors.affiliation: Department of Respiration, Children's Hospital Affiliated to Soochow University, Suzhou 215003, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Gu
authors.affiliation: Department of Respiration, Children's Hospital Affiliated to Soochow University, Suzhou 215003, China.
authors.forename: Xinxing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Respiration, Children's Hospital Affiliated to Soochow University, Suzhou 215003, China.
authors.forename: Yongdong
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Department of Respiration, Children's Hospital Affiliated to Soochow University, Suzhou 215003, China.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Respiration, Children's Hospital Affiliated to Soochow University, Suzhou 215003, China.
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Respiration, Children's Hospital Affiliated to Soochow University, Suzhou 215003, China.
authors.forename: Meijuan
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Clinical laboratory, Children's Hospital Affiliated to Soochow University, Suzhou 215003, China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Shao
authors.affiliation: Department of Respiration, Children's Hospital Affiliated to Soochow University, Suzhou 215003, China.
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Respiration, Children's Hospital Affiliated to Soochow University, Suzhou 215003, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ji
mesh_terms: D000818:Animals; D001249:Asthma / Q000097:blood / Q000378:metabolism*; D060887:B7 Antigens / Q000378:metabolism*; D001992:Bronchoalveolar Lavage Fluid; D049109:Cell Proliferation; D016207:Cytokines / Q000096:biosynthesis / Q000097:blood; D004195:Disease Models, Animal; D005260:Female; D017353:Gene Deletion; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism* / Q000473:pathology; D008168:Lung / Q000473:pathology; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism; D010047:Ovalbumin / Q000378:metabolism; D015398:Signal Transduction; D018417:Th1 Cells / Q000378:metabolism; D018418:Th2 Cells / Q000378:metabolism; D051195:Toll-Like Receptor 2 / Q000378:metabolism*
chemical_list: D060887:B7 Antigens; C486038:Cd276 protein, mouse; D016207:Cytokines; D016328:NF-kappa B; D051195:Toll-Like Receptor 2; D007073:Immunoglobulin E; D010047:Ovalbumin