title: Uncovering the SUMOylation and ubiquitylation crosstalk in human cells using sequential peptide immunopurification.
abstract: Crosstalk between the SUMO and ubiquitin pathways has recently been reported. However, no approach currently exists to determine the interrelationship between these modifications. Here, we report an optimized immunoaffinity method that permits the study of both protein ubiquitylation and SUMOylation from a single sample. This method enables the unprecedented identification of 10,388 SUMO sites in HEK293 cells. The sequential use of SUMO and ubiquitin remnant immunoaffinity purification facilitates the dynamic profiling of SUMOylated and ubiquitylated proteins in HEK293 cells treated with the proteasome inhibitor MG132. Quantitative proteomic analyses reveals crosstalk between substrates that control protein degradation, and highlights co-regulation of SUMOylation and ubiquitylation levels on deubiquitinase enzymes and the SUMOylation of proteasome subunits. The SUMOylation of the proteasome affects its recruitment to promyelocytic leukemia protein (PML) nuclear bodies, and PML lacking the SUMO interacting motif fails to colocalize with SUMOylated proteasome further demonstrating that this motif is required for PML catabolism.
authors.affiliation: Institute for Research in Immunology and Cancer, Université de Montréal, P.O. Box 6128, Station, Centre-ville, Montréal, Québec, Canada H3C 3J7.|Department of Chemistry, Université de Montréal, P.O. Box 6128, Station, Centre-ville, Montréal, Québec, Canada H3C 3J7.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Lamoliatte
authors.affiliation: Institute for Research in Immunology and Cancer, Université de Montréal, P.O. Box 6128, Station, Centre-ville, Montréal, Québec, Canada H3C 3J7.
authors.forename: Francis P
authors.initials: FP
authors.lastname: McManus
authors.affiliation: INSERM UMR-S1124, Université Paris Descartes, 75006 Paris, France.
authors.forename: Ghizlane
authors.initials: G
authors.lastname: Maarifi
authors.affiliation: INSERM UMR-S1124, Université Paris Descartes, 75006 Paris, France.
authors.forename: Mounira K
authors.initials: MK
authors.lastname: Chelbi-Alix
authors.affiliation: Institute for Research in Immunology and Cancer, Université de Montréal, P.O. Box 6128, Station, Centre-ville, Montréal, Québec, Canada H3C 3J7.|Department of Chemistry, Université de Montréal, P.O. Box 6128, Station, Centre-ville, Montréal, Québec, Canada H3C 3J7.|Department of Biochemistry, Université de Montréal, P.O. Box 6128, Station, Centre-ville, Montréal, Québec, Canada H3C 3J7.
authors.forename: Pierre
authors.initials: P
authors.lastname: Thibault
mesh_terms: D020816:Amino Acid Motifs; D002846:Chromatography, Affinity / Q000379:methods*; D057809:HEK293 Cells; D006801:Humans; D010455:Peptides / Q000737:chemistry*; D000071156:Promyelocytic Leukemia Protein / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex / Q000235:genetics / Q000378:metabolism; D060066:Protein Interaction Maps; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D059748:Proteolysis; D058207:Sumoylation; D025801:Ubiquitin / Q000737:chemistry; D054875:Ubiquitination
chemical_list: D010455:Peptides; D000071156:Promyelocytic Leukemia Protein; D011506:Proteins; D025801:Ubiquitin; D046988:Proteasome Endopeptidase Complex