title: G-CSF mobilized peripheral blood stem cell collection for allogeneic transplantation in healthy donors: Analysis of factors affecting yield.
abstract: Mobilized PBSC are the main source for allogeneic HSCT. We aimed to evaluate factors that affect CD34+ cell yield including the donor's age, gender, BSA, processed blood volume and the method of G-CSF dose calculation. Data from 170 healthy donors were analyzed. The concentration of CD34+ cells in the peripheral blood (PB) and the processed volume of blood were significantly correlated to CD34+ cells yield (P < .00005 and P < .001, respectively). The G-CSF dose per m2was significantly correlated to the concentration of CD34+ cells in the PB (P = .0003) and in the product (P = .01). Smaller BSA and less processed volume were found among female donors, who were given lesser G-CSF dose per m2, and showed lower yield compared to men. However, multivariate analysis of the yield showed that only the concentration of CD34+ cells in the PB and the processed volume remained independent significant.
authors.affiliation: Department of Medicine at Huddinge, Karolinska Institutet and Hematology Center Karolinska, Karolinska University Hospital, Stockholm, Sweden.
authors.forename: Maciej
authors.initials: M
authors.lastname: Machaczka
authors.affiliation: Department of Medicine at Huddinge, Karolinska Institutet and Hematology Center Karolinska, Karolinska University Hospital, Stockholm, Sweden.|Department of Medical Sciences, Hematology, Uppsala University, Uppsala, Sweden.
authors.forename: Hans
authors.initials: H
authors.lastname: Hägglund
authors.affiliation: Department of Medicine at Huddinge, Karolinska Institutet and Hematology Center Karolinska, Karolinska University Hospital, Stockholm, Sweden.
authors.forename: Emma
authors.initials: E
authors.lastname: Staver
authors.affiliation: Department of Hematology and Bone Marrow Transplantation, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Joks
authors.affiliation: Experimental Cancer Medicine, Clinical Research Centre, Department of Laboratory Medicine, Karolinska Institutet and Department of Clinical Research Centre, Karolinska University Hospital, Stockholm, Sweden.
authors.forename: Moustapha
authors.initials: M
authors.lastname: Hassan
authors.affiliation: Department of Medicine at Huddinge, Karolinska Institutet and Hematology Center Karolinska, Karolinska University Hospital, Stockholm, Sweden.
authors.forename: Björn Engelbrekt
authors.initials: BE
authors.lastname: Wahlin
authors.affiliation: Department of Medicine at Huddinge, Karolinska Institutet and Hematology Center Karolinska, Karolinska University Hospital, Stockholm, Sweden.|Department of Oncology and Pathology, Karolinska Institutet and Department of Clinical Immunology and Transfusion Medicine, Karolinska University Hospital, Stockholm, Sweden.
authors.forename: Ulla
authors.identifier: http://orcid.org/0000-0001-7892-3786
authors.initials: U
authors.lastname: Axdorph Nygell
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D018952:Antigens, CD34 / Q000032:analysis / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology* / Q000627:therapeutic use; D064368:Healthy Volunteers; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation / Q000379:methods*; D000072916:Peripheral Blood Stem Cells; D012737:Sex Factors; D014184:Transplantation, Homologous; D055815:Young Adult
chemical_list: D018952:Antigens, CD34; D016179:Granulocyte Colony-Stimulating Factor
keywords: G-CSF; PBSC donation; collection; healthy donors; mobilization