title: Specific and Sensitive Isothermal Electrochemical Biosensor for Plant Pathogen DNA Detection with Colloidal Gold Nanoparticles as Probes.
abstract: Developing quick and sensitive molecular diagnostics for plant pathogen detection is challenging. Herein, a nanoparticle based electrochemical biosensor was developed for rapid and sensitive detection of plant pathogen DNA on disposable screen-printed carbon electrodes. This 60 min assay relied on the rapid isothermal amplification of target pathogen DNA sequences by recombinase polymerase amplification (RPA) followed by gold nanoparticle-based electrochemical assessment with differential pulse voltammetry (DPV). Our method was 10,000 times more sensitive than conventional polymerase chain reaction (PCR)/gel electrophoresis and could readily identify P. syringae infected plant samples even before the disease symptoms were visible. On the basis of the speed, sensitivity, simplicity and portability of the approach, we believe the method has potential as a rapid disease management solution for applications in agriculture diagnostics.
authors.affiliation: Centre for Personalized Nanomedicine, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Australia.|Plant Genetic Engineering Laboratory, School of Agriculture and Food Sciences, The University of Queensland, Australia.
authors.forename: Han Yih
authors.initials: HY
authors.lastname: Lau
authors.affiliation: Centre for Personalized Nanomedicine, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Australia.|Department of Macromolecular Science, National Key Laboratory of polymer engineering, Fudan University, Shanghai, 200433, China.
authors.forename: Haoqi
authors.initials: H
authors.lastname: Wu
authors.affiliation: Centre for Personalized Nanomedicine, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Australia.
authors.forename: Eugene J H
authors.initials: EJ
authors.lastname: Wee
authors.affiliation: Centre for Personalized Nanomedicine, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Australia.|School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Matt
authors.initials: M
authors.lastname: Trau
authors.affiliation: Centre for Personalized Nanomedicine, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Australia.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Plant Genetic Engineering Laboratory, School of Agriculture and Food Sciences, The University of Queensland, Australia.
authors.forename: Jose R
authors.initials: JR
authors.lastname: Botella
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis*; D055664:Electrochemical Techniques / Q000379:methods*; D017979:Gold Colloid / Q000378:metabolism*; D053758:Nanoparticles / Q000378:metabolism*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D010935:Plant Diseases / Q000382:microbiology*; D044224:Pseudomonas syringae / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial; D017979:Gold Colloid