title: Alteration of CD39+Foxp3+ CD4 T cell and cytokine levels in EAE/MS following anti-CD52 treatment.
abstract: While examining the therapeutic value of anti-CD52 antibody against EAE/MS, we identified a unique subset of CD39+ Tregs in repopulating GALT tissues, a major lymphoid reservoir, which was accompanied by amelioration of disease. Furthermore, anti-CD52 treatment leads to increased expression of BDNF, IL-10, and SMAD3 in the brains of EAE mice. This condition is associated with suppression of IL-17, a critical inflammatory factor in EAE/MS progression. Additionally, we found elevated levels of CD4+CD39+ Tregs in PBMCs of RRMS patients treated with humanized anti-CD52 mAb. Thus, anti-CD52 can affect multiple immune mediated pathways involved in the pathogenesis of EAE/MS.
authors.affiliation: Department of Microbiology/Immunology, Geisel School of Medicine, Dartmouth College, Hanover, NH 03755, USA.
authors.forename: Anudeep B
authors.initials: AB
authors.lastname: Pant
authors.affiliation: Department of Microbiology/Immunology, Geisel School of Medicine, Dartmouth College, Hanover, NH 03755, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Microbiology/Immunology, Geisel School of Medicine, Dartmouth College, Hanover, NH 03755, USA.
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Mielcarz
authors.affiliation: Department of Microbiology/Immunology, Geisel School of Medicine, Dartmouth College, Hanover, NH 03755, USA.
authors.forename: Eli J
authors.initials: EJ
authors.lastname: Kasper
authors.affiliation: Department of Microbiology/Immunology, Geisel School of Medicine, Dartmouth College, Hanover, NH 03755, USA.
authors.forename: Kiel M
authors.initials: KM
authors.lastname: Telesford
authors.affiliation: Department of Microbiology/Immunology, Geisel School of Medicine, Dartmouth College, Hanover, NH 03755, USA.
authors.forename: Megan
authors.initials: M
authors.lastname: Mishra
authors.affiliation: Department of Microbiology/Immunology, Geisel School of Medicine, Dartmouth College, Hanover, NH 03755, USA. Electronic address: Azizul.Haque@Dartmouth.Edu.
authors.forename: Azizul
authors.initials: A
authors.lastname: Haque
authors.affiliation: Department of Microbiology/Immunology, Geisel School of Medicine, Dartmouth College, Hanover, NH 03755, USA.
authors.forename: Jacqueline Y
authors.initials: JY
authors.lastname: Channon
authors.affiliation: Department of Microbiology/Immunology, Geisel School of Medicine, Dartmouth College, Hanover, NH 03755, USA.
authors.forename: Lloyd H
authors.initials: LH
authors.lastname: Kasper
authors.affiliation: Department of Microbiology/Immunology, Geisel School of Medicine, Dartmouth College, Hanover, NH 03755, USA.
authors.forename: Sakhina
authors.initials: S
authors.lastname: Begum-Haque
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology / Q000627:therapeutic use*; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism*; D000951:Antigens, Neoplasm / Q000276:immunology / Q000378:metabolism*; D001081:Apyrase / Q000276:immunology / Q000378:metabolism*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D000074301:CD52 Antigen; D016207:Cytokines / Q000276:immunology / Q000378:metabolism*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy / Q000276:immunology / Q000378:metabolism*; D051858:Forkhead Transcription Factors / Q000276:immunology / Q000378:metabolism*; D006023:Glycoproteins / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D009103:Multiple Sclerosis / Q000188:drug therapy / Q000276:immunology / Q000378:metabolism; D016896:Treatment Outcome
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000074301:CD52 Antigen; C000613862:CD52 protein, human; D016207:Cytokines; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D006023:Glycoproteins; D001081:Apyrase; C067869:CD39 antigen
keywords: Anti-CD52 mAb; CD39+ T regulatory cells; Cytokine; Experimental autoimmune encephalomyelitis; Multiple sclerosis; Therapeutic