title: Protective effect of sugar cane extract against dextran sulfate sodium-induced colonic inflammation in mice.
abstract: Sugar cane extract (SCE) exhibits various biological effects and has been reported to enhance animal growth performance. However, the effect of SCE on inflammation in animals is still obscure. To study the effects and underlying mechanism of SCE on dextran sulfate sodium (DSS)-induced colonic inflammation, forty female ICR mice (26.63±0.19g, 6-week-old) were assigned into four groups: a control group (Cont), a DSS-challenged group (DSS), a SCE-supplemented group (SCE), and a DSS+SCE group (DSS+SCE). Mice in Cont group and DSS group were fed basic diet and other mice received 1% SCE supplemented in basic diet from 6-week to 8-week-old. Mice in DSS and DSS+SCE groups were also given a 4% DSS solution from 7-week to 8-week-old via drinking water to induce colonic inflammation. After 2 weeks, mice were sacrificed and samples were collected. The results showed that dietary SCE alleviated DSS induced growth suppression, splenic damage, colonic histological changes, colonic inflammation, oxidative stress, and colonic dysfunction of tight junctions. Meanwhile, the DSS exposure activated nuclear transcription factor kappa B p65 and inhibited nuclear factor E2-related factor 2 (Nrf2), while SCE markedly attenuated the DSS-promoted effect on the p65 nuclear accumulation and the DSS-inhibited effect on the Nrf2 nuclear accumulation. In conclusion, SCE conferred a protective role in the DSS-induced inflammation and the mechanism might be associated with the activated signals of the nuclear factor kappa B p65 and Nrf2.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China; Jiangsu Food & Pharmaceutical Science College, Huaian, 223005, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Yansen
authors.initials: Y
authors.lastname: Li
authors.affiliation: Product Development Division, Mitsui Sugar Co., Ltd., Tokyo, 103-8423, Japan.
authors.forename: Masami
authors.initials: M
authors.lastname: Mizu
authors.affiliation: Product Development Division, Mitsui Sugar Co., Ltd., Tokyo, 103-8423, Japan.
authors.forename: Toma
authors.initials: T
authors.lastname: Furuta
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: chunmeili@njau.edu.cn.
authors.forename: ChunMei
authors.initials: C
authors.lastname: Li
mesh_terms: D000818:Animals; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D016264:Dextran Sulfate / Q000633:toxicity; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051379:Mice; D051267:NF-E2-Related Factor 2 / Q000096:biosynthesis*; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D031786:Saccharum / Q000737:chemistry; D051996:Transcription Factor RelA / Q000096:biosynthesis*
chemical_list: D051267:NF-E2-Related Factor 2; C495636:Nfe2l2 protein, mouse; D010936:Plant Extracts; C498144:Rela protein, mouse; D051996:Transcription Factor RelA; D016264:Dextran Sulfate
keywords: Inflammation; Mouse; Oxidative stress; Sugar cane extract; Tight junction