title: Spatholobus suberectus Dunn. constituents inhibit sortase A and Staphylococcus aureus cell clumping to fibrinogen.
abstract: Sortases are a family of Gram-positive transpeptidases responsible for anchoring surface protein virulence factors to the peptidoglycan cell wall layer. In Staphylococcus aureus (S. aureus), deletion of sortase isoform results in a significant reduction in virulence and infection potential. Twenty flavonoids were isolated from the stem of the folk medicinal plant Spatholobus suberectus Dunn. These compounds were tested against S. aureus-derived sortase A (SrtA), a key transpeptidase for bacterial virulence. Among these active flavonoids, 7-hydroxy-6-methoxy-flavanone (3) and formononetin (10) were identified as compounds with promising SrtA inhibitory activity. These compounds also exhibited inhibitory activity against S. aureus cell clumping to fibrinogen. The suppression of cell-clumping activity indicates the potential of these compounds in the treatment of S. aureus infections via the inhibition of SrtA.
authors.affiliation: Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul, 151-742, Korea.
authors.forename: Hyunjoo
authors.initials: H
authors.lastname: Cho
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea.
authors.forename: Beomkoo
authors.initials: B
authors.lastname: Chung
authors.affiliation: Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul, 151-742, Korea.
authors.forename: Chang-Kwon
authors.initials: CK
authors.lastname: Kim
authors.affiliation: Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul, 151-742, Korea.
authors.forename: Dong-Chan
authors.initials: DC
authors.lastname: Oh
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea. ohkibong@snu.ac.kr.
authors.forename: Ki-Bong
authors.initials: KB
authors.lastname: Oh
authors.affiliation: Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul, 151-742, Korea. shinj@snu.ac.kr.
authors.forename: Jongheon
authors.initials: J
authors.lastname: Shin
mesh_terms: D019881:Aminoacyltransferases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007887:Fabaceae / Q000737:chemistry*; D005340:Fibrinogen / Q000037:antagonists & inhibitors* / Q000378:metabolism; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008968:Molecular Conformation; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D005419:Flavonoids; D005340:Fibrinogen; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases
keywords: Cell clumping; Flavonoids; Sortase A inhibition; Spatholobus suberectus Dunn.; Staphylococcus aureus