title: Modulation of the antibiotic activity against multidrug resistant strains of coumarins isolated from Rutaceae species.
abstract: The first occurrences and dissemination of resistant microorganisms led to the inefficacy of many antibiotics, available in the market nowadays, therefore, the search for new substances with antimicrobial activity from natural sources has gained a great importance. The purpose of this work is to evaluate the antibacterial activity and modulation of drug resistance in Staphylococcus aureus by coumarins such as bergapten, xantotoxin, isopimpinellin and imperatorin obtained from two Rutaceae species (Metrodorea mollis and Pilocarpus spicatus). The antimicrobial activity was assessed based on the minimum inhibitory concentration (MIC), using the microdilution method. The MIC was >256 g/mL for all coumarins tested. Regarding the modulation of drug resistance assay, the isopimpinellin reducted the MIC of erytromicin by 4 times, whereas imperatorin exhibited the best result, reducing the MIC of tetracycline (2 times), erytomicin (4 times) and norfloxacin (4 times). By reducing the MIC of ethidium bromide, the imperatorin is consider in fact, as a putative efflux pump inhibitor of bacteria.
authors.affiliation: Centro de Biotecnologia, Universidade Federal da Paraíba, João Pessoa, Brazil.
authors.forename: Sara A L
authors.initials: SA
authors.lastname: Madeiro
authors.affiliation: Departamento de Biologia Molecular, Universidade Federal da Paraíba, João Pessoa, Brazil.
authors.forename: Nathalie H P B
authors.initials: NH
authors.lastname: Borges
authors.affiliation: Departamento de Ciências Farmacêuticas, Universidade Federal da Paraíba, João Pessoa, Brazil.
authors.forename: Augusto L
authors.initials: AL
authors.lastname: Souto
authors.affiliation: Departamento de Ciências Farmacêuticas, Universidade Federal da Paraíba, João Pessoa, Brazil.
authors.forename: Pedro T R
authors.initials: PT
authors.lastname: de Figueiredo
authors.affiliation: Departamento de Biologia Molecular, Universidade Federal da Paraíba, João Pessoa, Brazil.
authors.forename: José P
authors.initials: JP
authors.lastname: Siqueira-Junior
authors.affiliation: Departamento de Ciências Farmacêuticas, Universidade Federal da Paraíba, João Pessoa, Brazil. Electronic address: josean@ltf.ufpb.br.
authors.forename: Josean F
authors.initials: JF
authors.lastname: Tavares
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D003374:Coumarins / Q000302:isolation & purification / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects*; D004357:Drug Synergism; D008826:Microbial Sensitivity Tests; D064209:Phytochemicals / Q000302:isolation & purification / Q000494:pharmacology*; D027821:Rutaceae / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D003374:Coumarins; D064209:Phytochemicals
keywords: Coumarins; Microbial resistance; Modulation; Rutaceae