title: Loss of Myh14 Increases Susceptibility to Noise-Induced Hearing Loss in CBA/CaJ Mice.
abstract: MYH14 is a member of the myosin family, which has been implicated in many motile processes such as ion-channel gating, organelle translocation, and the cytoskeleton rearrangement. Mutations in MYH14 lead to a DFNA4-type hearing impairment. Further evidence also shows that MYH14 is a candidate noise-induced hearing loss (NIHL) susceptible gene. However, the specific roles of MYH14 in auditory function and NIHL are not fully understood. In the present study, we used CRISPR/Cas9 technology to establish a Myh14 knockout mice line in CBA/CaJ background (now referred to as Myh14-/-mice) and clarify the role of MYH14 in the cochlea and NIHL. We found that Myh14-/-mice did not exhibit significant hearing loss until five months of age. In addition, Myh14-/-mice were more vulnerable to high intensity noise compared to control mice. More significant outer hair cell loss was observed in Myh14-/-mice than in wild type controls after acoustic trauma. Our findings suggest that Myh14 may play a beneficial role in the protection of the cochlea after acoustic overstimulation in CBA/CaJ mice.
authors.affiliation: School of Life Science and Key Laboratory of the Ministry of Education for Experimental Teratology, Shandong University, Jinan 250100, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Fu
authors.affiliation: School of Life Science and Key Laboratory of the Ministry of Education for Experimental Teratology, Shandong University, Jinan 250100, China.
authors.forename: Linqing
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Life Science and Key Laboratory of the Ministry of Education for Experimental Teratology, Shandong University, Jinan 250100, China.
authors.forename: Yecheng
authors.initials: Y
authors.lastname: Jin
authors.affiliation: School of Life Science and Key Laboratory of the Ministry of Education for Experimental Teratology, Shandong University, Jinan 250100, China.
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Sun
authors.affiliation: School of Life Science and Key Laboratory of the Ministry of Education for Experimental Teratology, Shandong University, Jinan 250100, China.
authors.forename: Aizhen
authors.initials: A
authors.lastname: Zhang
authors.affiliation: School of Life Science and Key Laboratory of the Ministry of Education for Experimental Teratology, Shandong University, Jinan 250100, China.
authors.forename: Zongzhuang
authors.initials: Z
authors.lastname: Wen
authors.affiliation: School of Life Science and Key Laboratory of the Ministry of Education for Experimental Teratology, Shandong University, Jinan 250100, China.
authors.forename: Yichen
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Second Hospital of Shandong University, Jinan 250033, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Xia
authors.affiliation: School of Life Science and Key Laboratory of the Ministry of Education for Experimental Teratology, Shandong University, Jinan 250100, China.
authors.forename: Jiangang
authors.identifier: 0000-0001-6619-7107
authors.initials: J
authors.lastname: Gao
mesh_terms: D000818:Animals; D001309:Auditory Threshold / Q000502:physiology*; D003051:Cochlea / Q000503:physiopathology*; D016057:Evoked Potentials, Auditory, Brain Stem / Q000235:genetics*; D005838:Genotype; D006317:Hearing Loss, Noise-Induced / Q000235:genetics / Q000503:physiopathology*; D051379:Mice; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D018995:Myosin Heavy Chains / Q000172:deficiency / Q000378:metabolism*; D024462:Myosin Type II / Q000172:deficiency / Q000378:metabolism*
chemical_list: C483905:Myh14 protein, mouse; D024462:Myosin Type II; D018995:Myosin Heavy Chains