title: Development of a Blocking ELISA Using a Monoclonal Antibody to a Dominant Epitope in Non-Structural Protein 3A of Foot-and-Mouth Disease Virus, as a Matching Test for a Negative-Marker Vaccine.
abstract: Foot-and-mouth disease (FMD) is a devastating animal disease. Strategies for differentiation of infected from vaccinated animals (DIVA) remain very important for controlling disease. Development of an epitope-deleted marker vaccine and accompanying diagnostic method will improve the efficiency of DIVA. Here, a monoclonal antibody (Mab) was found to recognize a conserved "AEKNPLE" epitope spanning amino acids 109-115 of non-structural protein (NSP) 3A of foot-and-mouth disease virus (FMDV; O/Tibet/CHA/99 strain), which could be deleted by a reverse-genetic procedure. In addition, a blocking ELISA was developed based on this Mab against NSP 3A, which could serve as a matching test for a negative-marker vaccine. The criterion of this blocking ELISA was determined by detecting panels of sera from different origins. The serum samples with a percentage inhibition (PI) equal or greater than 50% were considered to be from infected animals, and those with <50% PI were considered to be from non-infected animals. This test showed similar performance when compared with other 2 blocking ELISAs based on an anti-NSP 3B Mab. This is the first report of the DIVA test for an NSP antibody based on an Mab against the conserved and predominant "AEKNPLE" epitope in NSP 3A of FMDV.
authors.affiliation: Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science, State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Xujiaping, Yanchangpu, Lanzhou, Gansu, China.
authors.forename: Yuanfang
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science, State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Xujiaping, Yanchangpu, Lanzhou, Gansu, China.
authors.forename: Pinghua
authors.initials: P
authors.lastname: Li
authors.affiliation: Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science, State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Xujiaping, Yanchangpu, Lanzhou, Gansu, China.
authors.forename: Yimei
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science, State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Xujiaping, Yanchangpu, Lanzhou, Gansu, China.
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.affiliation: Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science, State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Xujiaping, Yanchangpu, Lanzhou, Gansu, China.
authors.forename: Pu
authors.initials: P
authors.lastname: Sun
authors.affiliation: Xinjiang Entry-Exit Inspection and Quarantine Bureau, Urumqi, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Shi
authors.affiliation: Xinjiang Entry-Exit Inspection and Quarantine Bureau, Urumqi, China.
authors.forename: Xincheng
authors.initials: X
authors.lastname: Ji
authors.affiliation: Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science, State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Xujiaping, Yanchangpu, Lanzhou, Gansu, China.
authors.forename: Huifang
authors.initials: H
authors.lastname: Bao
authors.affiliation: Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science, State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Xujiaping, Yanchangpu, Lanzhou, Gansu, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.affiliation: Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science, State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Xujiaping, Yanchangpu, Lanzhou, Gansu, China.
authors.forename: Yingli
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science, State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Xujiaping, Yanchangpu, Lanzhou, Gansu, China.
authors.forename: Xingwen
authors.initials: X
authors.lastname: Bai
authors.affiliation: Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science, State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Xujiaping, Yanchangpu, Lanzhou, Gansu, China.
authors.forename: Xueqing
authors.initials: X
authors.lastname: Ma
authors.affiliation: Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science, State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Xujiaping, Yanchangpu, Lanzhou, Gansu, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science, State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Xujiaping, Yanchangpu, Lanzhou, Gansu, China.
authors.forename: Zengjun
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science, State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Xujiaping, Yanchangpu, Lanzhou, Gansu, China.
authors.forename: Zaixin
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D000939:Epitopes / Q000276:immunology*; D005536:Foot-and-Mouth Disease / Q000175:diagnosis / Q000517:prevention & control*; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D011994:Recombinant Proteins; D012756:Sheep; D013552:Swine; D022581:Vaccines, Marker / Q000276:immunology; D017361:Viral Nonstructural Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D011994:Recombinant Proteins; D022581:Vaccines, Marker; D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines