title: High peroxidase-like activity of iron and nitrogen co-doped carbon dots and its application in immunosorbent assay.
abstract: Herein, iron and nitrogen co-doped carbon dots (Fe-N-CDs) were synthesized through a solvothermal method assisting with the microwave synthesis system for the first time. Multiple techniques were employed to characterize the properties of the obtained particles. The emphasis of current work was the confirmation of their intrinsic peroxidase activity due to the Fe doping, which is similar to natural ferriporphyrin. Through catalyzing the oxidization of 3,3',5,5'-Tetramethylbenzidine (TMB) in the presence of H2O2, the Fe-N-CDs exhibited a superior catalytic performance over horseradish peroxidase (HRP), suggesting their potential applications in catalytic area. Furthermore, the Fe-N-CDs were coupled to antibody as the biocatalyst instead of HRP (commonly used in enzyme-linked immunosorbnent assay (ELISA)). Results of carcinoembryonic antigen (CEA) determination demonstrated the feasibility of this approach, a detection limit as low as 0.1pg/mL within 5min has been achieved. These advances may trigger research interest in Fe-N-CDs and their new applications in various fields.
authors.affiliation: MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Yang
authors.affiliation: Fujian Provincial Key Laboratory of Modern Analytical Science and Separation Technology, College of Chemistry and Environment, Minnan Normal University, Zhangzhou 363000, China.
authors.forename: TingTing
authors.initials: T
authors.lastname: Huang
authors.affiliation: MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Zhao
authors.affiliation: MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Luo
authors.affiliation: Fujian Provincial Key Laboratory of Modern Analytical Science and Separation Technology, College of Chemistry and Environment, Minnan Normal University, Zhangzhou 363000, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Weng
authors.affiliation: MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China. Electronic address: qhw76@fzu.edu.cn.
authors.forename: Qiaohua
authors.initials: Q
authors.lastname: Wei
authors.affiliation: MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Lin
authors.affiliation: MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
keywords: Biocatalyst; Carbon dots; High peroxidase-like activity; Immunosorbent assay; Iron