title: MicroRNA-196a as a Potential Diagnostic Biomarker for Esophageal Squamous Cell Carcinoma.
abstract: We observed significant up-regulation of miR-196a in esophageal squamous cell carcinoma (ESCC) as compared with their adjacent normal tissue (p = .002). Receiver operating characteristics curve analysis confirmed the suitability of miR-196a as a potential tumor marker for diagnosis of ESCC. Furthermore, analysis of miR-196a levels in saliva samples determined an average of 27-fold up-regulations in ESCC patients compared with healthy group. Our results suggest that salivary miR-196a may be a suitable noninvasive biomarker for diagnosis of ESCC. In addition, molecular pathway enrichment analysis of microRNA (miR)-196a determined focal adhesion, spliceosome and p53 signaling pathways as the most relevant pathways with miR-196a targetome.
authors.affiliation: a Department of Biology, Faculty of Sciences , University of Isfahan , Isfahan , Iran.
authors.forename: Mona
authors.initials: M
authors.lastname: Fendereski
authors.affiliation: b Department of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences , Isfahan University of Medical Sciences , Gorgan , Iran.
authors.forename: Mohammad Farid
authors.initials: MF
authors.lastname: Zia
authors.affiliation: c Department of Medical Genetics, School of Advanced Medical Technologies , Golestan University of Medical Sciences , Gorgan , Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Shafiee
authors.affiliation: a Department of Biology, Faculty of Sciences , University of Isfahan , Isfahan , Iran.
authors.forename: Forousan
authors.initials: F
authors.lastname: Safari
authors.affiliation: d Department of Pathology , Isfahan University of Medical Sciences , Isfahan , Iran.
authors.forename: Mohammad Hosien
authors.initials: MH
authors.lastname: Saneie
authors.affiliation: a Department of Biology, Faculty of Sciences , University of Isfahan , Isfahan , Iran.
authors.forename: Manoochehr
authors.initials: M
authors.lastname: Tavassoli
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D004938:Esophageal Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D016612:Paraffin Embedding; D012372:ROC Curve; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C542543:MIRN196 microRNA, human; D035683:MicroRNAs
keywords: ESCC; biomarker; miR-196a; saliva