title: Polyelectrolyte Oil-Core Nanocarriers for Localized and Sustained Delivery of Daunorubicin to Colon Carcinoma MC38 Cells: The Case of Polysaccharide Multilayer Film in Relation to PEG-ylated Shell.
abstract: The authors examine properties of daunorubicin (DNR)-loaded oil-core multilayer nanocapsules prepared via layer-by-layer approach with different polyelectrolyte (PE) coatings such as a standard one (containing polysodium 4-styrenesulphonate/poly(diallyldimethyl-ammonium) chloride) and a polysaccharide-based shell (dextran/chitosan), in regard to the outer layer of poly-l-glutamic acid (PGA) grafted with polyethylene glycol (PGA-g-PEG). The nanocarriers are obtained on a cationic nanoemulsion template (stabilized by dicephalic-type surfactant, N,N-bis[3,30-(trimethylammonio)propyl]-dodecanamide dimethylsulfate) and layered with the PE shell of different thicknesses resulting in average size of 150 nm in diameter (as shown by dynamic light scattering, scanning electron microscopy and cryogenic-transmission electron microscopy, and atomic force microscopy). The nanocapsules demonstrate efficient DNR encapsulation and its sustained release under physiological conditions or in the attendance of human serum albumin. The biocompatibility studies using colon carcinoma MC38 and macrophage P388D1 cell lines as well as human erythrocytes reveal that surface charge and outer PE layer type determine nanocarrier features that control their biological activity: protein adsorption, cellular internalization and localization, induction of apoptosis, and hemolytic activity. The investigations indicate that polysaccharide-coated nanocapsules present a considerable potential for application as efficient DNR delivery systems in chemotherapy of colon cancer as an alternative to nanocarriers with PEG-ylated shell.
authors.affiliation: Department of Organic and Pharmaceutical Technology, Faculty of Chemistry, Wroclaw University of Science and Technology, Wybrzeze Wyspianskiego 27, 50-370, Wroclaw, Poland.
authors.forename: Urszula
authors.initials: U
authors.lastname: Bazylińska
authors.affiliation: Department of Medical Biochemistry, Medical University of Wroclaw, Chalubinskiego 10, 50-368, Wroclaw, Poland.
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Pietkiewicz
authors.affiliation: Institute of Immunology and Experimental Therapy Polish Academy of Sciences, Rudolfa Weigla 12, 53-114, Wroclaw, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Rossowska
authors.affiliation: Wroclaw Research Centre EIT+, Confocal Microscopy Laboratory, Stablowicka 147, 54-066, Wroclaw, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Chodaczek
authors.affiliation: Department of Medical Biochemistry, Medical University of Wroclaw, Chalubinskiego 10, 50-368, Wroclaw, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gamian
authors.affiliation: Department of Organic and Pharmaceutical Technology, Faculty of Chemistry, Wroclaw University of Science and Technology, Wybrzeze Wyspianskiego 27, 50-370, Wroclaw, Poland.
authors.forename: Kazimiera A
authors.initials: KA
authors.lastname: Wilk
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage*; D017209:Apoptosis / Q000187:drug effects; D001672:Biocompatible Materials; D002460:Cell Line; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000473:pathology*; D003630:Daunorubicin / Q000008:administration & dosage*; D004337:Drug Carriers; D004912:Erythrocytes / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles; D000071228:Polyelectrolytes / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D000903:Antibiotics, Antineoplastic; D001672:Biocompatible Materials; D004337:Drug Carriers; D000071228:Polyelectrolytes; D011134:Polysaccharides; D011092:Polyethylene Glycols; D003630:Daunorubicin
keywords: MC38 colon carcinoma cells; P388D1 macrophages; apoptotic activity; cellular internalization; hemolytic activity