title: Comprehensive analysis of draft genomes of two closely related pseudomonas syringae phylogroup 2b strains infecting mono- and dicotyledon host plants.
abstract: In recent years, the damage caused by bacterial pathogens to major crops has been increasing worldwide. Pseudomonas syringae is a widespread bacterial species that infects almost all major crops. Different P. syringae strains use a wide range of biochemical mechanisms, including phytotoxins and effectors of the type III and type IV secretion systems, which determine the specific nature of the pathogen virulence.
  
  
  
  Strains 1845 (isolated from dicots) and 2507 (isolated from monocots) were selected for sequencing because they specialize on different groups of plants. We compared virulence factors in these and other available genomes of phylogroup 2 to find genes responsible for the specialization of bacteria. We showed that strain 1845 belongs to the clonal group that has been infecting monocots in Russia and USA for a long time (at least 50 years). Strain 1845 has relatively recently changed its host plant to dicots.
  
  
  
  The results obtained by comparing the strain 1845 genome with the genomes of bacteria infecting monocots can help to identify the genes that define specific nature of the virulence of P. syringae strains.
authors.affiliation: Moscow Institute of Physics and Technology (State University), Moscow, Russia.|Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, the Russian Academy of Sciences, Moscow, Russia.
authors.forename: Rinat I
authors.initials: RI
authors.lastname: Sultanov
authors.affiliation: Moscow Institute of Physics and Technology (State University), Moscow, Russia.|Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, the Russian Academy of Sciences, Moscow, Russia.
authors.forename: Georgij P
authors.initials: GP
authors.lastname: Arapidi
authors.affiliation: Research Center of Biotechnology, Moscow, Russia.
authors.forename: Svetlana V
authors.initials: SV
authors.lastname: Vinogradova
authors.affiliation: Moscow Institute of Physics and Technology (State University), Moscow, Russia.|Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, the Russian Academy of Sciences, Moscow, Russia.|SRCC of Physical-Chemical Medicine, Moscow, Russia.
authors.forename: Vadim M
authors.initials: VM
authors.lastname: Govorun
authors.affiliation: USDA-ARS Foreign Disease - Weed Science Research Unit, Ft. Detrick, Washington, DC, USA.
authors.forename: Duglas G
authors.initials: DG
authors.lastname: Luster
authors.affiliation: Russian Peoples' Friendship University, Moscow, Russia. an.ignatov@gmail.com.|R&D Center "PhytoEngineering" LLC, Moscow region, Russia. an.ignatov@gmail.com.
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Ignatov
mesh_terms: D058947:Bacterial Secretion Systems / Q000235:genetics; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D019295:Computational Biology / Q000379:methods; D004251:DNA Transposable Elements; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing; D058977:Molecular Sequence Annotation; D005810:Multigene Family; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D044224:Pseudomonas syringae / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D053038:Quorum Sensing / Q000235:genetics; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics
chemical_list: D058947:Bacterial Secretion Systems; D004251:DNA Transposable Elements; D037521:Virulence Factors
keywords: Core genome; Dicots; Monocots; Pan-genome; Pseudomonas syringae; T3SS; Virulence factors