title: HPLC-UV method for simultaneous determination of MK-1775 and AZD-7762 in both acetonitrile-aqueous solution and mouse plasma.
abstract: A sensitive and precise method is described for the simultaneous determination of two small molecule kinase inhibitors: MK-1775 (MK) and AZD-7762 (AZD), in acetonitrile (ACN)-aqueous solution and in mouse plasma. A Nova-Pak C18 reversed phase column (3.9mm×150mm, 4μm, 60Å) was utilized in the separation using an isocratic mobile phase of 0.1% v/v triethylamine in phosphate buffer (pH=7.4): acetonitrile (ACN) (60:40, v/v), at a flow rate of 0.8mL/min. Detection wavelength was set at 310nm for both MK and AZD, and 431nm for the internal standard sunitinib (SUN). The developed method was validated following the ICH guidelines and it was shown to be accurate, precise and linear in the range of 41ng/mL to 8333ng/mL for both drugs in the ACN-aqueous solution and from 83ng/mL to 8333ng/mL for both drugs in mouse plasma samples. For the first time, the presented data suggest the suitability of this method for the simultaneous separation and quantification of MK and AZD in both ACN aqueous solution as well as in mouse plasma samples.
authors.affiliation: Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA 51141, USA.
authors.forename: Kareem
authors.initials: K
authors.lastname: Ebeid
authors.affiliation: Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA 51141, USA.
authors.forename: Giang N
authors.initials: GN
authors.lastname: Ho
authors.affiliation: Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA 51141, USA. Electronic address: aliasger-salem@uiowa.edu.
authors.forename: Aliasger K
authors.initials: AK
authors.lastname: Salem
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D057230:Limit of Detection; D016014:Linear Models; D051379:Mice; D008807:Mice, Inbred BALB C; D011720:Pyrazoles / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D011743:Pyrimidines / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D011744:Pyrimidinones; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D013056:Spectrophotometry, Ultraviolet; D013876:Thiophenes / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D014508:Urea / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000737:chemistry
chemical_list: C532363:3-(carbamoylamino)-5-(3-fluorophenyl)-N-(3-piperidyl)thiophene-2-carboxamide; D011720:Pyrazoles; D011743:Pyrimidines; D011744:Pyrimidinones; D013876:Thiophenes; D014508:Urea; C549567:adavosertib
keywords: AZD-7762; HPLC-UV; MK-1775; Mouse plasma; Solid phase extraction; Sunitinib