title: MicroRNA-409-3p Represses Glioma Cell Invasion and Proliferation by Targeting High-Mobility Group Nucleosome-Binding Domain 5.
abstract: Emerging evidence has suggested that aberrantly expressed microRNAs (miRNAs) are associated with glioma development and progression. The aberrant expression of miR-409-3p has been reported in several human cancers. However, little is known about the function of miR-409-3p in gliomas. The aim of this study was to investigate the specific role and molecular mechanism of miR-409-3p in gliomas. In the present study, we found that miR-409-3p was downregulated in glioma tissue and cell lines. Overexpression of miR-409-3p inhibited glioma cell invasion and proliferation, whereas suppression of miR-409-3p promoted glioma cell invasion and proliferation. High-mobility group nucleosome-binding domain 5 (HMGN5), a well-known oncogene in gliomas, was identified as a functional target of miR-409-3p using bioinformatics, dual-luciferase reporter assay, real-time quantitative polymerase chain reaction, and Western blot analysis. Furthermore, miR-409-3p was found to regulate the expression of matrix metalloproteinase 2 and cyclin D1. Restoration of HMGN5 expression significantly reversed the inhibitory effects of miR-409-3p overexpression on glioma cell invasion and proliferation. Taken together, our results suggest that miR-409-3p inhibits glioma cell invasion and proliferation by targeting HMGN5, representing a potential therapeutic target for glioma.
authors.forename: Yidong
authors.initials: Y
authors.lastname: Cao
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Minghao
authors.initials: M
authors.lastname: Wei
authors.forename: Xue
authors.initials: X
authors.lastname: Jiang
authors.forename: Dong
authors.initials: D
authors.lastname: Jia
mesh_terms: D020413:3' Untranslated Regions; D001932:Brain Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D005910:Glioma / Q000235:genetics*; D024001:HMGN Proteins / Q000235:genetics*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics*; D034622:RNA Interference; D015534:Trans-Activators / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D024001:HMGN Proteins; C429289:HMGN5 protein, human; C572367:MIRN409 microRNA, human; D035683:MicroRNAs; D015534:Trans-Activators; D019938:Cyclin D1; D020778:Matrix Metalloproteinase 2