title: Novel SNPs inIL-17FandIL-17Agenes associated with somatic cell count in Chinese Holstein and Inner-Mongolia Sanhe cattle.
abstract: BACKGROUND
  Bovine mastitis is the most common and costly disease of lactating cattle worldwide. Apart from milk somatic cell count (SCC) and somatic cell score (SCS), serum cytokines such as interleukin-17 (IL-17) and interleukin-4 (IL-4) may also be potential indicators for bovine mastitis. The present study was designed to investigate the effects of single nucleotide polymorphisms (SNPs) in bovineIL-17FandIL-17Agenes on SCC, SCS and serum cytokines in Chinese Holstein and Inner-Mongolia Sanhe cattle, and to compare the mRNA expression variations of the cows with different genotypes.
  
  
  RESULTS
  A total of 464 lactating cows (337 Holstein and 127 Inner-Mongolia Sanhe cattle) were screened for SNPs identification and the data were analyzed using fixed effects of herd, parity, season and year of calving by general linear model procedure. The results revealed that SNP g.24392436C > T inIL-17Fand SNP g.24345410A > G inIL-17Ashowed significant effects on SCC and IL-4 in Holstein (n= 337) and on IL-17 and IL-4 in Sanhe cattle (n= 127). The homozygous GG genotype of SNP g.24345410A > G had significantly higher mRNA expression compared with the heterozygous AG genotype.
  
  
  CONCLUSIONS
  The results indicate thatIL-17FandIL-17Acould be powerful candidate genes of mastitis resistance and the significant SNPs might be useful genetic markers against mastitis in both dairy and dual purpose cattle.
authors.affiliation: Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture of China, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 People's Republic of China.|College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University Mardan, Mardan, 23200 Pakistan.
authors.forename: Tahir
authors.initials: T
authors.lastname: Usman
authors.affiliation: Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture of China, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 People's Republic of China.
authors.forename: Yachun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture of China, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.affiliation: Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture of China, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 People's Republic of China.
authors.forename: Yanghua
authors.initials: Y
authors.lastname: He
authors.affiliation: Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture of China, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture of China, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 People's Republic of China.
authors.forename: Yichun
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Xieerltala Breeding Farm, Hailaer, 021012 Inner Mongolia China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Wu
authors.affiliation: Agricultural and Animal Husbandry Administration Bureau, Hailaer, 021000 Inner Mongolia China.
authors.forename: Airong
authors.initials: A
authors.lastname: Liu
authors.affiliation: Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture of China, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yu
keywords: Chinese Holstein; Inner Mongolia Sanhe cattle; Interleukin 17A; Interleukin 17F; Mastitis susceptibility