title: Punicalagin Pretreatment Attenuates Myocardial Ischemia-Reperfusion Injury via Activation of AMPK.
abstract: Punicalagin (PUN), a major bioactive component in pomegranate juice, has been proven to exert neuroprotective effects against cerebral ischemia/reperfusion (I/R) insult via anti-oxidant properties. This study aims to investigate whether PUN provides cardioprotection against myocardial I/R (MI/R) injury and the underlying mechanisms. PUN (30[Formula: see text]mg/kg/d) or vehicle was intragastrically administered to Sprague-Dawley rats for one week before the operation. MI/R was induced by ligating the left anterior descending coronary artery for 30[Formula: see text]min and subsequent reperfusion for 3[Formula: see text]h. PUN pretreatment conferred cardioprotective effects against MI/R injury by improving cardiac function, limiting infarct size, reducing serum creatine kinase-MB and lactate dehydrogenase activities, and suppressing cardiomyocyte apoptosis. Moreover, PUN pretreatment inhibited I/R-induced myocardial oxidative stress as evidenced by decreased generation of superoxide content and malonaldialdehyde formation and increased antioxidant capability. Furthermore, PUN pretreatment increased adenosine monophosphate-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) phosphorylation in I/R hearts. AMPK inhibitor compound c inhibited PUN-enhanced AMPK phosphorylation, and blunted PUN-mediated anti-oxidative effects and cardioprotection. These results indicate for the first time that PUN pretreatment protect against I/R-induced oxidative stress and myocardial injury via activation of AMPK.
authors.affiliation: * Department of Geriatrics, Xijing Hospital, Fourth Military Medical University, Xi'an, P.R. China.|§ Department of Geriatrics, Xi'an Central Hospital, Xi'an, P.R. China.
authors.forename: Mingge
authors.initials: M
authors.lastname: Ding
authors.affiliation: ‡ Department of Cardiology, Traditional Chinese Medicine Hospital of Baoji City, Baoji, P.R. China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: ¶ Department of Cardiology, Shengli Oilfield Central Hospital, Dongying, P.R. China.
authors.forename: Di
authors.initials: D
authors.lastname: Sun
authors.affiliation: † Department of Physiology, Fourth Military Medical University, Xi'an, P.R. China.
authors.forename: Zhenghua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: † Department of Physiology, Fourth Military Medical University, Xi'an, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: * Department of Geriatrics, Xijing Hospital, Fourth Military Medical University, Xi'an, P.R. China.
authors.forename: Xing
authors.initials: X
authors.lastname: Li
authors.affiliation: * Department of Geriatrics, Xijing Hospital, Fourth Military Medical University, Xi'an, P.R. China.
authors.forename: Cong
authors.initials: C
authors.lastname: Huo
authors.affiliation: * Department of Geriatrics, Xijing Hospital, Fourth Military Medical University, Xi'an, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Jia
authors.affiliation: ¶ Department of Cardiology, Shengli Oilfield Central Hospital, Dongying, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: † Department of Physiology, Fourth Military Medical University, Xi'an, P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Fu
authors.affiliation: * Department of Geriatrics, Xijing Hospital, Fourth Military Medical University, Xi'an, P.R. China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Wang
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002316:Cardiotonic Agents / Q000494:pharmacology; D003331:Coronary Vessels / Q000601:surgery*; D052279:Creatine Kinase, MB Form / Q000187:drug effects / Q000378:metabolism; D004195:Disease Models, Animal; D006321:Heart / Q000187:drug effects*; D047348:Hydrolyzable Tannins / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D008026:Ligation; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000473:pathology; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D032383:Myocytes, Cardiac / Q000187:drug effects; D018384:Oxidative Stress; D011292:Premedication; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D002316:Cardiotonic Agents; D047348:Hydrolyzable Tannins; C115642:punicalagin; D007770:L-Lactate Dehydrogenase; D055372:AMP-Activated Protein Kinases; D052279:Creatine Kinase, MB Form
keywords: AMPK; Myocardial Ischemia/Reperfusion; Oxidative Stress; Punicalagin