title: Assessment of Neuronal Cell-Based Cytotoxicity of Neurotoxins from an Estuarine Nemertean in the Han River Estuary.
abstract: A heteronemertean,Yininemertes pratensis, was collected in Han River Estuary, South Korea. This estuarine nemertean has been known by the local fishermen for harmful effects to the glass eels, juveniles of Japanese eelAnguilla japonica, migrating to fresh water. The present study confirmed the neurotoxic effects of this heteronemertean ribbon worm at the cellular level. Derivative types of neurotoxic tetrodotoxin (TTX), 5,11-dideoxy TTX (m/z288) and 11-norTTX-6(S)-01 (m/z305.97), were identified through HPLC and MALDI-TOF MS. However, significant neurotoxicity was confirmed in the fraction containing an undefined molecule corresponding to the 291.1 (m/z) peak, when tested in rat primary astrocytes and dorsal ganglion cells. This study is the first to report neurotoxins of the estuarine nemertean, fairly abundant in the Han River estuary, and suggests the long-term monitoring of population dynamics and surveillance of the toxicity in this river estuary.
authors.affiliation: Department of Biological Engineering, Inha University, Incheon 22212, Republic of Korea.
authors.forename: Yeo Seon
authors.initials: YS
authors.lastname: Kwon
authors.affiliation: Department of Biological Engineering, Inha University, Incheon 22212, Republic of Korea.
authors.forename: Seul Ki
authors.initials: SK
authors.lastname: Min
authors.affiliation: Department of Biological Engineering, Inha University, Incheon 22212, Republic of Korea.
authors.forename: Seung Ju
authors.initials: SJ
authors.lastname: Yeon
authors.affiliation: Department of Biological Engineering, Inha University, Incheon 22212, Republic of Korea.
authors.forename: Jin Ha
authors.initials: JH
authors.lastname: Hwang
authors.affiliation: Department of Oceanography, Inha University, Incheon 22212, Republic of Korea.
authors.forename: Jae-Sang
authors.initials: JS
authors.lastname: Hong
authors.affiliation: Department of Biological Engineering, Inha University, Incheon 22212, Republic of Korea.
authors.forename: Hwa Sung
authors.initials: HS
authors.lastname: Shin
mesh_terms: D000811:Anguilla / Q000254:growth & development / Q000502:physiology; D000818:Animals; D001253:Astrocytes / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004784:Environmental Monitoring; D063366:Estuaries; D005618:Fresh Water; D009498:Neurotoxins / Q000737:chemistry* / Q000633:toxicity*; D051381:Rats; D056910:Republic of Korea; D045483:Rivers; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013779:Tetrodotoxin / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D009498:Neurotoxins; D014874:Water Pollutants, Chemical; D013779:Tetrodotoxin
keywords: Heteronemertean; Korean Han River estuary; Yininemertes pratensis; neurotoxicity; tetrodotoxin