title: Suppression of PDCD4 mediated by the long non-coding RNA HOTAIR inhibits the proliferation and invasion of glioma cells.
abstract: Programmed cell death protein 4 (PDCD4) has recently been demonstrated to be implicated in translation and transcription, and the regulation of cell growth. However, the mechanisms underlying PDCD4 function in glioma cells remain to be elucidated. The current study investigated the function and regulation of PDCD4 and the results demonstrated that the expression of PDCD4 was significantly reduced in glioma cells compared with normal cells. When PDCD4 was overexpressed in glioma cells, the proliferation rate and invasive capability of the cells greatly decreased, suggesting that PDCD4 functions as a tumor suppressor in this cell type. In addition, the histone modification status of the PDCD4 gene was analyzed, and chromatin immunoprecipitation assay identified a high density of histone 3 lysine 27 trimethylation on the promoter of PDCD4, which was associated with the long non-coding RNA, homeobox transcript antisense RNA (HOTAIR). The expression of HOTAIR was significantly increased in glioma cells compared with normal cells, and it exerted its function in a polycomb repressive complex 2-dependent manner. These results may provide novel approaches to therapeutically target PDCD4 and HOTAIR in patients with gliomas.
authors.affiliation: Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China; Emergency Centre, Yantai Yuhuangding Hospital Affiliated to Qingdao University Medical College, Yantai, Shandong 264000, P.R. China.
authors.forename: Yong'An
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China; Emergency Centre, Yantai Yuhuangding Hospital Affiliated to Qingdao University Medical College, Yantai, Shandong 264000, P.R. China.
authors.forename: Yusong
authors.initials: Y
authors.lastname: Bian
authors.affiliation: Emergency Centre, Yantai Yuhuangding Hospital Affiliated to Qingdao University Medical College, Yantai, Shandong 264000, P.R. China.
authors.forename: Shanpeng
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Emergency Centre, Yantai Yuhuangding Hospital Affiliated to Qingdao University Medical College, Yantai, Shandong 264000, P.R. China.
authors.forename: Fanqiang
authors.initials: F
authors.lastname: Kong
authors.affiliation: Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China.
authors.forename: Xin'Gang
authors.initials: X
authors.lastname: Li
keywords: glioma; homeobox transcript antisense RNA; long non-coding RNA; programmed cell death protein 4