title: Schisandra chinensis and Its Main Constituent Schizandrin Attenuate Allergic Reactions by Down-Regulating Caspase-1 in Ovalbumin-Sensitized Mice.
abstract: Schisandra chinensis (SC) and its main constituent, schizandrin (SCH) exhibit anti-inflammatory and anti-allergic activities. Allergic and inflammatory reactions are aggravated via caspase-1 signaling pathway. However, the regulatory effects of SC and SCH on caspase-1 activation have not been clarified yet. In this study, we aimed to clarify the anti-allergic effects of SC and SCH using an ovalbumin (OVA)-sensitized mice and anti-CD3 and anti-CD28 antibodies-stimulated splenocytes. SC or SCH significantly inhibited the levels of immunoglobulin (Ig)E, IgG1, or interleukin (IL)-4 in serum of OVA-sensitized mice. SC or SCH significantly inhibited the levels of IL-6, tumor necrosis factor (TNF)-[Formula: see text], and IL-1[Formula: see text] in spleen of the OVA-sensitized mice. SC or SCH significantly suppressed the expression of caspase-1 and receptor-interacting protein (RIP)-2 in spleen of the OVA-sensitized mice. In activated splenocytes, SC or SCH significantly decreased the expression of caspase-1 and RIP-2 as well as the production of IL-6 and TNF-[Formula: see text]. We suggest that SC and SCH exert an anti-allergic effect by down-regulating caspase-1 signaling.
authors.affiliation: * Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
authors.forename: Na-Ra
authors.initials: NR
authors.lastname: Han
authors.affiliation: * Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
authors.forename: Phil-Dong
authors.initials: PD
authors.lastname: Moon
authors.affiliation: * Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
authors.forename: Na-Rae
authors.initials: NR
authors.lastname: Kim
authors.affiliation: * Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
authors.forename: Hee-Yun
authors.initials: HY
authors.lastname: Kim
authors.affiliation: † Department of Food Science & Technology and Research Institute for Basic Science, Hoseo University, Chungnam 31499, Republic of Korea.
authors.forename: Hyun-Ja
authors.initials: HJ
authors.lastname: Jeong
authors.affiliation: * Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
mesh_terms: D000818:Animals; D020170:Caspase 1 / Q000187:drug effects* / Q000378:metabolism; D034242:Cyclooctanes / Q000494:pharmacology*; D015536:Down-Regulation; D006967:Hypersensitivity / Q000276:immunology*; D007073:Immunoglobulin E / Q000187:drug effects / Q000276:immunology; D007074:Immunoglobulin G / Q000187:drug effects / Q000276:immunology; D053583:Interleukin-1beta / Q000187:drug effects / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D017705:Lignans / Q000494:pharmacology*; D051379:Mice; D010047:Ovalbumin; D010936:Plant Extracts / Q000494:pharmacology*; D011083:Polycyclic Compounds / Q000494:pharmacology*; D053475:Receptor-Interacting Protein Serine-Threonine Kinase 2; D053422:Receptor-Interacting Protein Serine-Threonine Kinases / Q000187:drug effects / Q000378:metabolism; D032141:Schisandra; D013154:Spleen / Q000166:cytology / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects / Q000276:immunology
chemical_list: D034242:Cyclooctanes; C587672:IL1B protein, mouse; D007074:Immunoglobulin G; D053583:Interleukin-1beta; D015850:Interleukin-6; D017705:Lignans; D010936:Plant Extracts; D011083:Polycyclic Compounds; D014409:Tumor Necrosis Factor-alpha; C577686:interleukin-6, mouse; D015847:Interleukin-4; D007073:Immunoglobulin E; D010047:Ovalbumin; D053475:Receptor-Interacting Protein Serine-Threonine Kinase 2; D053422:Receptor-Interacting Protein Serine-Threonine Kinases; C506255:Ripk2 protein, mouse; D020170:Caspase 1; C011105:schizandrin
keywords: Allergic Reaction; Caspase-1; Schisandra chinensis; Schizandrin; Splenocytes