title: NMR Techniques in Metabolomic Studies: A Quick Overview on Examples of Utilization.
abstract: Metabolomics is a rapidly developing branch of science that concentrates on identifying biologically active molecules with potential biomarker properties. To define the best biomarkers for diseases, metabolomics uses both models (in vitro, animals) and human, as well as, various techniques such as mass spectroscopy, gas chromatography, liquid chromatography, infrared and UV-VIS spectroscopy and nuclear magnetic resonance. The last one takes advantage of the magnetic properties of certain nuclei, such as1H,13C,31P,19F, especially their ability to absorb and emit energy, what is crucial for analyzing samples. Among many spectroscopic NMR techniques not only one-dimensional (1D) techniques are known, but for many years two-dimensional (2D, for example, COSY, DOSY, JRES, HETCORE, HMQS), three-dimensional (3D, DART-MS, HRMAS, HSQC, HMBC) and solid-state NMR have been used. In this paper, authors taking apart fundamental division of nuclear magnetic resonance techniques intend to shown their wide application in metabolomic studies, especially in identifying biomarkers.
authors.affiliation: Department of Organic Chemistry, Faculty of Pharmacy, Poznan University of Medical Sciences, Grunwaldzka 6 Str., 60-780 Poznan, Poland.
authors.forename: Joanna
authors.identifier: 0000-0002-7517-5345
authors.initials: J
authors.lastname: Kruk
authors.affiliation: RootInnovation Sp. z o.o., Jana Matejki 11 Str., 50-333 Wrocław, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Doskocz
authors.affiliation: Department of Organic Chemistry, Faculty of Pharmacy, Poznan University of Medical Sciences, Grunwaldzka 6 Str., 60-780 Poznan, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Jodłowska
authors.affiliation: Department of Organic Chemistry, Faculty of Pharmacy, Poznan University of Medical Sciences, Grunwaldzka 6 Str., 60-780 Poznan, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Zacharzewska
authors.affiliation: Department of Organic Chemistry, Faculty of Pharmacy, Poznan University of Medical Sciences, Grunwaldzka 6 Str., 60-780 Poznan, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Łakomiec
authors.affiliation: Department of Organic Chemistry, Faculty of Pharmacy, Poznan University of Medical Sciences, Grunwaldzka 6 Str., 60-780 Poznan, Poland.
authors.forename: Kornelia
authors.initials: K
authors.lastname: Czaja
authors.affiliation: Department of Organic Chemistry, Faculty of Pharmacy, Poznan University of Medical Sciences, Grunwaldzka 6 Str., 60-780 Poznan, Poland ; Foundation for Development of Science and Business on Medical and Exact Sciences Area, Legnicka 65 Str., 54-206 Wrocław, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Kujawski