title: Human p53 interacts with the elongating RNAPII complex and is required for the release of actinomycin D induced transcription blockage.
abstract: The p53 tumour suppressor regulates the transcription initiation of selected genes by binding to specific DNA sequences at their promoters. Here we report a novel role of p53 in transcription elongation in human cells. Our data demonstrate that upon transcription elongation blockage, p53 is associated with genes that have not been reported as its direct targets. p53 could be co-immunoprecipitated with active forms of DNA-directed RNA polymerase II subunit 1 (RPB1), highlighting its association with the elongating RNA polymerase II. During a normal transcription cycle, p53 and RPB1 are localised at distinct regions of selected non-canonical p53 target genes and this pattern of localisation was changed upon blockage of transcription elongation. Additionally, transcription elongation blockage induced the proteasomal degradation of RPB1. Our results reveal a novel role of p53 in human cells during transcription elongation blockage that may facilitate the removal of RNA polymerase II from DNA.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, 6726, Hungary.
authors.forename: Barbara N
authors.initials: BN
authors.lastname: Borsos
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, 6726, Hungary.
authors.forename: Ildikó
authors.initials: I
authors.lastname: Huliák
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, 6726, Hungary.
authors.forename: Hajnalka
authors.initials: H
authors.lastname: Majoros
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, 6726, Hungary.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Ujfaludi
authors.affiliation: Department of Molecular Genetics, Erasmus University Medical Centre, Rotterdam, PO Box 2040, 3000 CA, The Netherlands.
authors.forename: Ákos
authors.initials: Á
authors.lastname: Gyenis
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, 6726, Hungary.
authors.forename: Peter
authors.initials: P
authors.lastname: Pukler
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, 6726, Hungary.|Institute of Biochemistry, Biological Research Centre, Szeged, 6726, Hungary.
authors.forename: Imre M
authors.initials: IM
authors.lastname: Boros
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, 6726, Hungary.
authors.forename: Tibor
authors.initials: T
authors.lastname: Pankotai