title: Structural proteins ofEnterococcus faecalisbacteriophageϕEf11.
abstract: ϕEf11, a temperateSiphoviridaebacteriophage, was isolated by induction from a root canal isolate ofEnterococcus faecalis. Sequence analysis suggested that the ϕEf11 genome included a contiguous 8 gene module whose function was related to head structure assembly and another module of 10 contiguous genes whose products were responsible for tail structure assembly. SDS-PAGE analysis of virions of a ϕEf11 derivative revealed 11 well-resolved protein bands. To unify the deduced functional gene assignments emanating from the DNA sequence data, with the structural protein analysis of the purified virus, 6 of the SDS-PAGE bands were subjected to mass spectrometry analysis. 5 of the 6 protein bands analyzed by mass spectrometry displayed identical amino acid sequences to those predicted to be specified by 4 of the ORFs identified in the ϕEf11 genome. These included: ORF8 (predicted scaffold protein), ORF10 (predicted major head protein), ORF15 (predicted major tail protein), and ORF23 (presumptive antireceptor).
authors.affiliation: Laboratory of Oral Infectious Diseases, Temple University Kornberg School of Dentistry, Philadelphia, PA, USA; Department of Endodontics, Temple University Kornberg School of Dentistry, Philadelphia, PA, USA.
authors.forename: Roy H
authors.initials: RH
authors.lastname: Stevens
authors.affiliation: Laboratory of Oral Infectious Diseases, Temple University Kornberg School of Dentistry, Philadelphia, PA, USA; Department of Endodontics, Temple University Kornberg School of Dentistry, Philadelphia, PA, USA.
authors.forename: Hongming
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Laboratory of Oral Infectious Diseases, Temple University Kornberg School of Dentistry , Philadelphia, PA, USA.
authors.forename: Chaiwing
authors.initials: C
authors.lastname: Hsiao
authors.affiliation: Department of Oral Biology, Rutgers School of Dental Medicine, Rutgers University , Newark, NJ, USA.
authors.forename: Scott
authors.initials: S
authors.lastname: Kachlany
authors.affiliation: Department of Periodontology, UERJ/Unigranrio , Rio de Janeiro, Brazil.
authors.forename: Eduardo M B
authors.initials: EM
authors.lastname: Tinoco
authors.affiliation: Department of Genomic Medicine, J Craig Venter Institute , Rockville, MD, USA.
authors.forename: Jessica
authors.initials: J
authors.lastname: DePew
authors.affiliation: Department of Genomic Medicine, J Craig Venter Institute , Rockville, MD, USA.
authors.forename: Derrick E
authors.initials: DE
authors.lastname: Fouts
keywords: Enterococcus faecalis; bacteriophage; phage ϕEf11