title: African and Asian Zika virus strains differentially induce early antiviral responses in primary human astrocytes.
abstract: ZIKA virus (ZIKV) is a newly emerging arbovirus. Since its discovery 60years ago in Uganda, it has spread throughout the Pacific, Latin America and the Caribbean, emphasizing the capacity of ZIKV to spread to non-endemic regions worldwide. Although infection with ZIKV often leads to mild disease, its recent emergence in the Americas has coincided with an increase in adults developing Guillain-Barré syndrome and neurological complications in new-borns, such as congenital microcephaly. Many questions remain unanswered regarding the complications caused by different primary isolates of ZIKV. Here, we report the permissiveness of primary human astrocytes for two clinically relevant, Asian and African ZIKV strains and show that both isolates strongly induce antiviral immune responses in these cells albeit with markedly different kinetics. This study describes for the first time the specific antiviral gene expression in infected primary human astrocytes, the major glial cells within the central nervous system.
authors.affiliation: Laboratoire MIVEGEC, UMR 224 IRD/CNRS/UM, Montpellier, France.
authors.forename: Rodolphe
authors.initials: R
authors.lastname: Hamel
authors.affiliation: Laboratoire MIVEGEC, UMR 224 IRD/CNRS/UM, Montpellier, France.
authors.forename: Pauline
authors.initials: P
authors.lastname: Ferraris
authors.affiliation: Laboratoire MIVEGEC, UMR 224 IRD/CNRS/UM, Montpellier, France.
authors.forename: Sineewanlaya
authors.initials: S
authors.lastname: Wichit
authors.affiliation: Laboratoire MIVEGEC, UMR 224 IRD/CNRS/UM, Montpellier, France.
authors.forename: Fodé
authors.initials: F
authors.lastname: Diop
authors.affiliation: Laboratoire MIVEGEC, UMR 224 IRD/CNRS/UM, Montpellier, France.
authors.forename: Loïc
authors.initials: L
authors.lastname: Talignani
authors.affiliation: Laboratoire MIVEGEC, UMR 224 IRD/CNRS/UM, Montpellier, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Pompon
authors.affiliation: Laboratoire MIVEGEC, UMR 224 IRD/CNRS/UM, Montpellier, France.
authors.forename: Déborah
authors.initials: D
authors.lastname: Garcia
authors.affiliation: Laboratoire MIVEGEC, UMR 224 IRD/CNRS/UM, Montpellier, France.
authors.forename: Florian
authors.initials: F
authors.lastname: Liégeois
authors.affiliation: Institut Pasteur, Dakar, Senegal.
authors.forename: Amadou A
authors.initials: AA
authors.lastname: Sall
authors.affiliation: Centre d'Immunologie et des Maladies Infectieuses, INSERM, U1135, Sorbonne Universités, UPMC, APHP Hôpital Pitié-Salpêtrière, Paris, France.
authors.forename: Hans
authors.initials: H
authors.lastname: Yssel
authors.affiliation: Laboratoire MIVEGEC, UMR 224 IRD/CNRS/UM, Montpellier, France. Electronic address: dorothee.misse@ird.fr.
authors.forename: Dorothée
authors.initials: D
authors.lastname: Missé
mesh_terms: D001253:Astrocytes / Q000276:immunology* / Q000821:virology; D000071457:DEAD Box Protein 58 / Q000235:genetics / Q000276:immunology*; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D006801:Humans; D007113:Immunity, Innate; D000070576:NLR Proteins / Q000235:genetics / Q000276:immunology*; D061251:Primary Cell Culture; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics; D011971:Receptors, Immunologic; D013997:Time Factors; D051193:Toll-Like Receptors / Q000235:genetics / Q000276:immunology*; D019562:Viral Load / Q000276:immunology; D014779:Virus Replication / Q000276:immunology; D000071244:Zika Virus / Q000235:genetics / Q000254:growth & development
chemical_list: D000070576:NLR Proteins; D012367:RNA, Viral; D011971:Receptors, Immunologic; D051193:Toll-Like Receptors; C452820:RIGI protein, human; D000071457:DEAD Box Protein 58
keywords: Antiviral response; Arbovirus; Astrocytes; Innate immunity; ZIKA