title: A label-free electrochemical immunosensor based on a new polymer containing aldehyde and ferrocene groups.
abstract: In this work, a novel and convenient strategy for synthesis of a well-defined polymer containing both aldehyde and ferrocene functional groups by grafting 4-hydroxybenzaldehyde and ferrocenecarboxylhydrazide onto linear poly (vinylbenzyl chloride) was developed. The designed polymer film (polymer CAF) could be used as a new support matrix for fabrication of a label-free electrochemical immunosensor with high sensitivity, good stability and repeatability. With human IgG as an analyte model, the proposed electrochemical immunosensor showed a linear concentration range from 0.1 to 20ngmL-1with a detection limit of 0.07ngmL-1(S/N=3). Moreover, interfering proteins including HSA, IgM, and IgE were added into the analyte, the developed immnnosensor can detect human IgG with high specificity.
authors.affiliation: Hunan Province Cooperative Innovation Center for the Construction & Development of Dongting Lake Ecological Economic Zone, College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, ChangDe 415000, PR China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Hunan Province Cooperative Innovation Center for the Construction & Development of Dongting Lake Ecological Economic Zone, College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, ChangDe 415000, PR China; Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China. Electronic address: ymshen79@163.com.
authors.forename: Youming
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China. Electronic address: zhangyy@hunnu.edu.cn.
authors.forename: Youyu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Hunan Province Cooperative Innovation Center for the Construction & Development of Dongting Lake Ecological Economic Zone, College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, ChangDe 415000, PR China.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Shen
authors.affiliation: Hunan Province Cooperative Innovation Center for the Construction & Development of Dongting Lake Ecological Economic Zone, College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, ChangDe 415000, PR China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Xiang
authors.affiliation: Hunan Province Cooperative Innovation Center for the Construction & Development of Dongting Lake Ecological Economic Zone, College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, ChangDe 415000, PR China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Long
keywords: Electrochemical immunosensor; Good performance; Label-free; New matrix