title: Bone marrow mesenchymal stem cells inhibit dendritic cells differentiation and maturation by microRNA-23b.
abstract: Research on regulation and its mechanism of bone marrow mesenchymal stem cells (BMSCs) on dendritic cells (DCs), which is the initiating factor in immune response has applicable clinical value. Although BMSCs have a significant regulatory effect on the maturation of DCs, its molecular mechanism is still unclear. BMSCs and DCs, were co-cultured by different concentration ratios. Flow cytometry was used to detect the expression of DC markers (CD83, CD11c). Quantitative reverse transcription PCR (qRT-PCR) was used to measure the expression of related genes in RNA level. Expression of the target proteins was detected with using Western blot assay. miRNA inhibitor and miRNA mimic were used to suppress and up-regulate the expression of the target gene. In this research, our results demonstrated that BMSCs notably inhibited maturation of DCs in the co-culture system of BMSCs and DCs and confirmed that this inhibition is due to overexpression ofmiR-23bFurthermore, this research found thatmiR-23boverexpression inhibited the expression of p50/p65, thus blocked the activation of the NF-κB pathway. In conclusion, BMSCs affected the activation of NF-κB pathway throughmiR-23boverexpression resulting in inhibition of the maturation and differentiation of DCs.
authors.affiliation: Department of Orthopedics, Affiliated Hospital of Taishan Medical University, Taian 271000, Shandong Province, China.
authors.forename: Jingguo
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Orthopedics, Taian City Centre Hospital, Taian, 271000, Shandong Province, China.
authors.forename: Chengbo
authors.initials: C
authors.lastname: Ji
authors.affiliation: Department of Nursing, Taian City Centre Hospital Branch, Taian, 271000, Shandong Province, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Cao
authors.affiliation: Department of Nursing, Taian City Centre Hospital Branch, Taian, 271000, Shandong Province, China.
authors.forename: Hongfen
authors.initials: H
authors.lastname: Lui
authors.affiliation: Department of Orthopedics, Taian City Centre Hospital, Taian, 271000, Shandong Province, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Xia
authors.affiliation: Department of Orthopedics, Affiliated Hospital of Taishan Medical University, Taian 271000, Shandong Province, China fywanglanping123@126.com xiabo1975@163.com.
authors.forename: Lanping
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D003713:Dendritic Cells / Q000378:metabolism / Q000502:physiology*; D015870:Gene Expression; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D015854:Up-Regulation / Q000502:physiology
chemical_list: D035683:MicroRNAs; C534062:Mirn23b microRNA, mouse; D016328:NF-kappa B
keywords: NF-κB pathway; bone marrow mesenchymal stem cells (BMSCs); dendritic cells (DCs); immune tolerance; maturation; miR-23b