title: Effect of Different Lignocellulosic Diets on Bacterial Microbiota and Hydrolytic Enzyme Activities in the Gut of the Cotton Boll Weevil (Anthonomus grandis).
abstract: Cotton boll weevils,Anthonomus grandis, are omnivorous coleopteran that can feed on diets with different compositions, including recalcitrant lignocellulosic materials. We characterized the changes in the prokaryotic community structure and the hydrolytic activities ofA. grandislarvae fed on different lignocellulosic diets.A. grandislarvae were fed on three different artificial diets: cottonseed meal (CM), Napier grass (NG) and corn stover (CS). Total DNA was extracted from the gut samples for amplification and sequencing of the V3-V4 hypervariable region of the 16S rRNA gene. Proteobacteria and Firmicutes dominated the gut microbiota followed by Actinobacteria, Spirochaetes and a small number of unclassified phyla in CM and NG microbiomes. In the CS feeding group, members of Spirochaetes were the most prevalent, followed by Proteobacteria and Firmicutes. Bray-Curtis distances showed that the samples from the CS community were clearly separated from those samples of the CM and NG diets. Gut extracts from all three diets exhibited endoglucanase, xylanase, β-glucosidase and pectinase activities. These activities were significantly affected by pH and temperature across different diets. We observed that the larvae reared on a CM showed significantly higher activities than larvae reared on NG and CS. We demonstrated that the intestinal bacterial community structure varies depending on diet composition. Diets with more variable and complex compositions, such as CS, showed higher bacterial diversity and richness than the two other diets. In spite of the detected changes in composition and diversity, we identified a core microbiome shared between the three different lignocellulosic diets. These results suggest that feeding with diets of different lignocellulosic composition could be a viable strategy to discover variants of hemicellulose and cellulose breakdown systems.
authors.affiliation: Instituto de Biotecnología, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Centro Nacional de Investigaciones Agropecuarias - Instituto Nacional de Tecnología Agropecuaria Castelar Hurlingham, Argentina.
authors.forename: Emiliano
authors.initials: E
authors.lastname: Ben Guerrero
authors.affiliation: Instituto de Investigaciones en Biociencias Agrícolas y Ambientales-Consejo Nacional de Investigaciones Científicas y Técnicas, Cátedra de Microbiología Agrícola, Facultad de Agronomía, Universidad de Buenos Aires Buenos Aires, Argentina.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Soria
authors.affiliation: Instituto de Microbiología y Zoología Agrícola, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Centro Nacional de Investigaciones Agropecuarias - Instituto Nacional de Tecnología Agropecuaria Castelar Hurlingham, Argentina.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Salvador
authors.affiliation: Earth and Environmental Sciences, Lawrence Berkeley National Laboratory Berkeley, CA, USA.
authors.forename: Javier A
authors.initials: JA
authors.lastname: Ceja-Navarro
authors.affiliation: Instituto de Biotecnología, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Centro Nacional de Investigaciones Agropecuarias - Instituto Nacional de Tecnología Agropecuaria CastelarHurlingham, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)Buenos Aires, Argentina.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Campos
authors.affiliation: Earth and Environmental Sciences, Lawrence Berkeley National Laboratory Berkeley, CA, USA.
authors.forename: Eoin L
authors.initials: EL
authors.lastname: Brodie
authors.affiliation: Instituto de Biotecnología, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Centro Nacional de Investigaciones Agropecuarias - Instituto Nacional de Tecnología Agropecuaria CastelarHurlingham, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)Buenos Aires, Argentina.
authors.forename: Paola
authors.initials: P
authors.lastname: Talia
keywords: 16S rRNA gene; Anthonomus grandis; gut microbiota; hydrolytic activities; illumina amplicon sequencing; lignocellulosic feedstocks