title: Ftr82 Is Critical for Vascular Patterning during Zebrafish Development.
abstract: Cellular components and signaling pathways are required for the proper growth of blood vessels. Here, we report for the first time that a teleost-specific geneftr82(finTRIM family, member 82) plays a critical role in vasculature during zebrafish development. To date, there has been no description of tripartite motif proteins (TRIM) in vascular development, and the role offtr82is unknown. In this study, we found thatftr82mRNA is expressed during the development of vessels, and loss offtr82by morpholino (MO) knockdown impairs the growth of intersegmental vessels (ISV) and caudal vein plexus (CVP), suggesting thatftr82plays a critical role in promoting ISV and CVP growth. We showed the specificity offtr82MO by analyzingftr82expression products and expressingftr82mRNA to rescueftr82morphants. We further showed that the knockdown offtr82reduced ISV cell numbers, suggesting that the growth impairment of vessels is likely due to a decrease of cell proliferation and migration, but not cell death. In addition, loss offtr82affects the expression of vascular markers, which is consistent with the defect of vascular growth. Finally, we showed thatftr82likely interacts with vascular endothelial growth factor (VEGF) and Notch signaling. Together, we identify teleost-specificftr82as a vascular gene that plays an important role for vascular development in zebrafish.
authors.affiliation: Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. changhw@kmu.edu.tw.|Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. changhw@kmu.edu.tw.|Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. changhw@kmu.edu.tw.|Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan. changhw@kmu.edu.tw.
authors.forename: Hsueh-Wei
authors.initials: HW
authors.lastname: Chang
authors.affiliation: Department of Bioagricultural Science, National Chiayi University, Chiayi City 60004, Taiwan. wangw4@mail.ncyu.edu.tw.
authors.forename: Wen-Der
authors.initials: WD
authors.lastname: Wang
authors.affiliation: Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. cchiu@kmu.edu.tw.|Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. cchiu@kmu.edu.tw.
authors.forename: Chien-Chih
authors.initials: CC
authors.lastname: Chiu
authors.affiliation: Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. z333134@hotmail.com.
authors.forename: Chiou-Hua
authors.initials: CH
authors.lastname: Chen
authors.affiliation: Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. ki200412@yahoo.com.tw.
authors.forename: Yi-Shan
authors.initials: YS
authors.lastname: Wang
authors.affiliation: Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. ying8215@gmail.com.
authors.forename: Zih-Ying
authors.initials: ZY
authors.lastname: Chen
authors.affiliation: Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. liuwangta@kmu.edu.tw.
authors.forename: Wangta
authors.initials: W
authors.lastname: Liu
authors.affiliation: Institute of Biomedical Science, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. minghongtai@gmail.com.|Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University and Academia Sinica, Kaohsiung 80424, Taiwan. minghongtai@gmail.com.
authors.forename: Ming-Hong
authors.initials: MH
authors.lastname: Tai
authors.affiliation: Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University and Academia Sinica, Kaohsiung 80424, Taiwan. wzh@mail.nsysu.edu.tw.|Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. wzh@mail.nsysu.edu.tw.
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Wen
authors.affiliation: Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. cywu@mail.nsysu.edu.tw.|Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. cywu@mail.nsysu.edu.tw.|Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. cywu@mail.nsysu.edu.tw.|Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University and Academia Sinica, Kaohsiung 80424, Taiwan. cywu@mail.nsysu.edu.tw.
authors.forename: Chang-Yi
authors.initials: CY
authors.lastname: Wu
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001775:Blood Circulation; D019521:Body Patterning / Q000235:genetics; D004487:Edema / Q000473:pathology; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D018919:Neovascularization, Physiologic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051880:Receptors, Notch / Q000378:metabolism; D015398:Signal Transduction; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D015415:Biomarkers; C000617857:Ftr82 protein, zebrafish; D012333:RNA, Messenger; D051880:Receptors, Notch; D029961:Zebrafish Proteins; D040301:Vascular Endothelial Growth Factor Receptor-2
keywords: CVP (caudal vein plexus); ISV (intersegmental vessel); TRIM family; angiogenesis; ftr82; zebrafish