title: Antichlamydial Dimeric Indole Derivatives from Marine Actinomycete Rubrobacter radiotolerans.
abstract: Chlamydiae are widely distributed pathogens of human populations, which can lead to serious reproductive and other health problems. In our search for novel antichlamydial metabolites from marine derived-microorganisms, one new (1) and two known (2, 3) dimeric indole derivatives were isolated from the sponge-derived actinomyceteRubrobacter radiotolerans. The chemical structures of these metabolites were elucidated by NMR spectroscopic data as well as CD calculations. All three metabolites suppressed chlamydial growth in a concentration-dependent manner. Among them, compound1exhibited the most effective antichlamydial activity with IC50values of 46.6 ~ 96.4 µM in the production of infectious progeny. Compounds appeared to target the mid-stage of the chlamydial developmental cycle by interfering with reticular body replication, but not directly inactivating the infectious elementary body.
authors.affiliation: School of Pharmacy, Nantong University, Nantong, China.
authors.forename: Jian Lin
authors.initials: JL
authors.lastname: Li
authors.affiliation: School of Pharmacy, Nantong University, Nantong, China.|Department of Pharmacy, The Second People's Hospital of Nantong, Nantong, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Chen
authors.affiliation: School of Pharmacy, Nantong University, Nantong, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.affiliation: School of Pharmacy, Nantong University, Nantong, China.
authors.forename: Min
authors.initials: M
authors.lastname: Ni
authors.affiliation: School of Pharmacy, Nantong University, Nantong, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Robert Wood Johnson Medical School, Rutgers University, Piscataway, New Jersey, USA.
authors.forename: Huizhou
authors.initials: H
authors.lastname: Fan
authors.affiliation: School of Pharmacy, Nantong University, Nantong, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Bao
mesh_terms: D039903:Actinobacteria / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology / Q000633:toxicity; D002692:Chlamydia trachomatis / Q000187:drug effects* / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D007211:Indoles / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D015394:Molecular Structure; D047130:Petrosia / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D007211:Indoles