title: In Situ Metabolomics in Cancer by Mass Spectrometry Imaging.
abstract: Metabolomics is a rapidly evolving and a promising research field with the expectation to improve diagnosis, therapeutic treatment prediction, and prognosis of particular diseases. Among all techniques used to assess the metabolome in biological systems, mass spectrometry imaging is the method of choice to qualitatively and quantitatively analyze metabolite distribution in tissues with a high spatial resolution, thus providing molecular data in relation to cancer histopathology. The technique is ideally suited to study tissues molecular content and is able to provide molecular biomarkers or specific mass signatures which can be used in classification or the prognostic evaluation of tumors. Recently, it was shown that FFPE tissue samples are also suitable for metabolic analyses. This progress in methodology allows access to a highly valuable resource of tissues believed to widen and strengthen metabolic discovery-driven studies.
authors.affiliation: Research Unit Analytical Pathology, Helmholtz Zentrum München, Neuherberg, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Buck
authors.affiliation: Research Unit Analytical Pathology, Helmholtz Zentrum München, Neuherberg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Aichler
authors.affiliation: Research Unit Analytical Pathology, Helmholtz Zentrum München, Neuherberg, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Huber
authors.affiliation: Research Unit Analytical Pathology, Helmholtz Zentrum München, Neuherberg, Germany. Electronic address: axel.walch@helmholtz-muenchen.de.
authors.forename: A
authors.initials: A
authors.lastname: Walch
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor
keywords: Diagnostic; FFPE tissue; MALDI; Mass spectrometry imaging; Metabolomics; Predictive; Prognostic pathology