title: A Bioorganometallic Approach to Study Histidine Kinase Autophosphorylations.
abstract: Auto-phosphorylation of bacterial histidine kinases PhoR, PhoQ, and EnvZ has been investigated using adenosine-5'-[γ-ferrocene] triphosphate (Fc-ATP) as a cosubstrate for the first time. The study has been carried out in solution and on surface. Results from biochemical multiplex assay and surface electrochemical/optical methods are consistent, which successfully demonstrates that Fc-ATP is an efficient cosubstrate for histidine kinase auto-phosphorylations. The study also has discovered that the concentration of Fc-ATP influences the autophosphorylation efficiency. This developed methodology will provide a powerful tool in studying such biological processes towards further understanding of the involved mechanism.
authors.affiliation: Beijing Key Laboratory of Photoelectronic/, Electrophotonic Conversion Materials, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, 100081, P. R. China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.affiliation: Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, Ontario, M5S 3H6, Canada.
authors.forename: Zhe
authors.initials: Z
authors.lastname: She
authors.affiliation: Department of Physical and Environmental Science, University of Toronto Scarborough, 1265 Military Trail, Toronto, Ontario, M1C 1A4, Canada.
authors.forename: Zakiyya
authors.initials: Z
authors.lastname: Ingar
authors.affiliation: Department of Chemistry, Oakland University, 2200 North Squirrel Road, Rochester, Michigan, 48309, USA.
authors.forename: Sanela
authors.initials: S
authors.lastname: Martic
authors.affiliation: Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, Ontario, M5S 3H6, Canada.
authors.forename: Heinz-Bernhard
authors.identifier: http://orcid.org/0000-0002-7149-0110
authors.initials: HB
authors.lastname: Kraatz
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000378:metabolism; D001419:Bacteria / Q000201:enzymology* / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D055664:Electrochemical Techniques / Q000379:methods; D057075:Enzyme Assays / Q000379:methods; D005296:Ferrous Compounds / Q000737:chemistry / Q000378:metabolism; D000071677:Histidine Kinase / Q000378:metabolism*; D000075163:Metallocenes / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D005296:Ferrous Compounds; D000075163:Metallocenes; C079792:PhoQ protein, Bacteria; C061279:PhoR protein, Bacteria; D000255:Adenosine Triphosphate; D000071677:Histidine Kinase; C004998:ferrocene
keywords: electrochemistry; ferrocene; fluorescence; histidine kinase; phosphorylation