title: Netrin-1 overexpression improves neurobehavioral outcomes and reduces infarct size via inhibition of the notch1 pathway following experimental stroke.
abstract: Stroke is a leading cause of morbidity and mortality worldwide. Administration of Netrin-1 during the peri-infarct period has been shown to decrease infarct size in rats; however, the underlying mechanism is unclear. We addressed this question in the present study by inducing stroke in rats via middle cerebral artery occlusion (MCAO), and evaluating the effects of Netrin-1 treatment by neurobehavioral testing, immunocytochemistry, and western blotting. Netrin-1 overexpression increased neurobehavioral test scores and reduced cerebral infarct volume following MCAO via inhibition of the Notch1 signaling pathway. These results demonstrate that early administration of Netrin-1 can is an effective therapeutic approach for improving outcome after stroke. © 2017 Wiley Periodicals, Inc.
authors.affiliation: Department of Neurosurgery, XinHua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China.
authors.forename: Xiaosheng
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, XinHua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China.
authors.forename: Shiting
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, XinHua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, XinHua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China.
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Second Affiliated Hospital of Zhejiang University Medical College, Hangzhou, 310009, China.
authors.forename: Chongran
authors.initials: C
authors.lastname: Sun
authors.affiliation: Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100050, China.
authors.forename: Haiqiang
authors.initials: H
authors.lastname: Qin
authors.affiliation: Department of Neurosurgery, XinHua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D008297:Male; D000075388:Netrin-1 / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D051881:Receptor, Notch1 / Q000378:metabolism*; D020521:Stroke / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D015854:Up-Regulation
chemical_list: C497763:Notch1 protein, rat; D051881:Receptor, Notch1; D000075388:Netrin-1
keywords: Netrin-1; Notch1 pathway; middle cerebral artery occlusion; neurobehavioral