title: Biocatalysis with Unnatural Amino Acids: Enzymology Meets Xenobiology.
abstract: The goal of xenobiology is to design biological systems endowed with unusual biochemical functions, whereas enzymology concerns the study of enzymes, the workhorses of biocatalysis. Biocatalysis employs enzymes and organisms to perform useful biotransformations in synthetic chemistry and biotechnology. During the past few years, the effects of incorporating noncanonical amino acids (ncAAs) into enzymes with potential applications in biocatalysis have been increasingly investigated. In this Review, we provide an overview of the effects of new chemical functionalities that have been introduced into proteins to improve various facets of enzymatic catalysis. We also discuss future research avenues that will complement unnatural mutagenesis with standard protein engineering to produce novel and versatile biocatalysts with applications in synthetic organic chemistry and biotechnology.
authors.affiliation: Institut für Chemie, Technische Universität Berlin, Müller-Breslau-Strasse 10, 10623, Berlin, Germany.|Institute of Chemistry and Biochemistry-Organic Chemistry, Freie Universität Berlin, Takustrasse 3, 14195, Berlin, Germany.
authors.forename: Federica
authors.initials: F
authors.lastname: Agostini
authors.affiliation: Institut für Chemie, Technische Universität Berlin, Müller-Breslau-Strasse 10, 10623, Berlin, Germany.
authors.forename: Jan-Stefan
authors.initials: JS
authors.lastname: Völler
authors.affiliation: Institute of Chemistry and Biochemistry-Organic Chemistry, Freie Universität Berlin, Takustrasse 3, 14195, Berlin, Germany.
authors.forename: Beate
authors.initials: B
authors.lastname: Koksch
authors.affiliation: Biosyntia ApS, 2100, Copenhagen, Denmark.
authors.forename: Carlos G
authors.initials: CG
authors.lastname: Acevedo-Rocha
authors.affiliation: Institut für Chemie, Technische Universität Berlin, Müller-Breslau-Strasse 10, 10623, Berlin, Germany.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Kubyshkin
authors.affiliation: Institut für Chemie, Technische Universität Berlin, Müller-Breslau-Strasse 10, 10623, Berlin, Germany.
authors.forename: Nediljko
authors.initials: N
authors.lastname: Budisa
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D001709:Biotechnology; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004256:DNA Polymerase I / Q000378:metabolism*; D006801:Humans; D009601:Nitroreductases / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism*; D015202:Protein Engineering
chemical_list: D000596:Amino Acids; D003577:Cytochrome P-450 Enzyme System; D010544:Peroxidases; D009601:Nitroreductases; D004256:DNA Polymerase I
keywords: bioorthogonality; biotransformations; enzyme catalysis; synthetic biology; unnatural amino acids