title: Responses of the rotifer Brachionus plicatilis to flame retardant (BDE-47) stress.
abstract: A series of short-term toxicological tests were conducted on the rotifer Brachionus plicatilis to assess the toxicity of the flame retardant 2,2',4,4'-tetrabrominated biphenyl ether (BDE-47). BDE-47 increased mortality, morphological damage, and altered population dynamics and fecundity of rotifer. Antioxidant enzymes were differentially changed to maintain the balance between antioxidant and pro-oxidant activity. However, with increases in the concentration of BDE-47, the metabolic and antioxidant activity decreased. Moreover, the reactive oxygen species (ROS) and malondialdehyde contents increased and the ratio between glutathione and glutathione-SH decreased, indicating oxidative stress. The addition of the ROS-inhibitor N-acetylcysteine alleviated the degree of damage and stimulated the activity of xenobiotic-metabolizing and antioxidant system, which suggested that ROS were the most important loop in the stress response.
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao, Shandong Province 266003, China.
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Jian
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao, Shandong Province 266003, China.
authors.forename: Xuexi
authors.initials: X
authors.lastname: Tang
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao, Shandong Province 266003, China.
authors.forename: Ningning
authors.initials: N
authors.lastname: Xu
authors.affiliation: North China Sea Environmental Monitoring Center, State Oceanic Administration, Fushun Road 22, Qingdao, Shandong Province 266033, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Sha
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao, Shandong Province 266003, China. Electronic address: wangyou@ouc.edu.cn.
authors.forename: You
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D005411:Flame Retardants / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D012403:Rotifera / Q000187:drug effects*; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D005411:Flame Retardants; D055768:Halogenated Diphenyl Ethers; D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; D005978:Glutathione
keywords: 2,2′,4,4′-Tetrabromodiphenyl ether (BDE-47); Antioxidant enzymatic system; Brachionus plicatilis; Marine zooplankton; Reactive oxygen species (ROS); Xenobiotic-metabolizing enzymatic system