title: Antibacterial efficacy of triple-layered poly(lactic-co-glycolic acid)/nanoapatite/lauric acid guided bone regeneration membrane on periodontal bacteria.
abstract: A guided bone regeneration (GBR) membrane has been extensively used in the repair and regeneration of damaged periodontal tissues. One of the main challenges of GBR restoration is bacterial colonization on the membrane, constitutes to premature membrane degradation. Therefore, the purpose of this study was to investigate the antibacterial efficacy of triple-layered GBR membrane composed of poly(lactic-co-glycolic acid) (PLGA), nanoapatite (NAp) and lauric acid (LA) with two types of Gram-negative periodontal bacteria, Fusobacterium nucleatum and Porphyromonas gingivalis through a disc diffusion and bacterial count tests. The membranes exhibited a pattern of growth inhibition and killing effect against both bacteria. The increase in LA concentration tended to increase the bactericidal activities which indicated by higher diameter of inhibition zone and higher antibacterial percentage. It is shown that the incorporation of LA into the GBR membrane has retarded the growth and proliferation of Gram-negative periodontal bacteria for the treatment of periodontal disease.
authors.affiliation: Faculty of Biosciences & Medical Engineering, Universiti Teknologi Malaysia.
authors.forename: Nur Najiha
authors.initials: NN
authors.lastname: Saarani
authors.affiliation: Faculty of Biosciences & Medical Engineering, Universiti Teknologi Malaysia.|Advanced Materials Research Center.
authors.forename: Kalitheerta
authors.initials: K
authors.lastname: Jamuna-Thevi
authors.affiliation: Industrial Biotechnology Research Center, SIRIM Berhad.
authors.forename: Neelam
authors.initials: N
authors.lastname: Shahab
authors.affiliation: Department of Mining, Metallurgical and Materials Engineering & CHU de Québec Research Center, Laval University.
authors.forename: Hendra
authors.initials: H
authors.lastname: Hermawan
authors.affiliation: Faculty of Biosciences & Medical Engineering, Universiti Teknologi Malaysia.
authors.forename: Syafiqah
authors.initials: S
authors.lastname: Saidin
mesh_terms: D000900:Anti-Bacterial Agents; D001031:Apatites; D001419:Bacteria; D001861:Bone Regeneration; D019344:Lactic Acid; D007850:Lauric Acids; D008567:Membranes, Artificial; D010517:Periodontics; D011108:Polymers
chemical_list: D000900:Anti-Bacterial Agents; D001031:Apatites; D007850:Lauric Acids; D008567:Membranes, Artificial; D011108:Polymers; D019344:Lactic Acid
keywords: Antibacterial; Guided bone regeneration; Lauric acid; Periodontal bacteria