title: A novel dual-functional biosensor for fluorometric detection of inorganic pyrophosphate and pyrophosphatase activity based on globulin stabilized gold nanoclusters.
abstract: A novel ultrasensitive dual-functional biosensor for highly sensitive detection of inorganic pyrophosphate (PPi) and pyrophosphatase (PPase) activity was developed based on the fluorescent variation of globulin protected gold nanoclusters (Glo@Au NCs) with the assistance of Cu2+. Glo@Au NCs and PPi were used as the fluorescent indicator and substrate for PPase activity evaluation, respectively. In the presence of Cu2+, the fluorescence of the Glo@Au NCs will be quenched owing to the formation of Cu2+-Glo@Au NCs complex, while PPi can restore the fluorescence of the Cu2+-Glo@Au NCs complex because of its higher binding affinity with Cu2+. As PPase can catalyze the hydrolysis of PPi, it will lead to the release of Cu2+and re-quench the fluorescence of the Glo@Au NCs. Based on this mechanism, quantitative evaluation of the PPi and PPase activity can be achieved ranging from 0.05 μM to 218.125 μM for PPi and from 0.1 to 8 mU for PPase, with detection limits of 0.02 μM and 0.04 mU, respectively, which is much lower than that of other PPi and PPase assay methods. More importantly, this ultrasensitive dual-functional biosensor can also be successfully applied to evaluate the PPase activity in human serum, showing great promise for practical diagnostic applications.
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
authors.forename: Shenghao
authors.initials: S
authors.lastname: Xu
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
authors.forename: Xiuying
authors.initials: X
authors.lastname: Feng
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
authors.forename: Teng
authors.initials: T
authors.lastname: Gao
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China; College of Polymer Science and Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
authors.forename: Ruizhi
authors.initials: R
authors.lastname: Wang
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
authors.forename: Yaning
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
authors.forename: Jiehua
authors.initials: J
authors.lastname: Lin
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
authors.forename: Xijuan
authors.initials: X
authors.lastname: Yu
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China. Electronic address: xiliangluo@qust.edu.cn.
authors.forename: Xiliang
authors.initials: X
authors.lastname: Luo
mesh_terms: D015374:Biosensing Techniques; D011756:Diphosphates / Q000032:analysis*; D005916:Globulins; D006046:Gold; D053768:Metal Nanoparticles; D011755:Pyrophosphatases / Q000032:analysis*
chemical_list: D011756:Diphosphates; D005916:Globulins; D006046:Gold; D011755:Pyrophosphatases
keywords: Competitive response; Dual-functional biosensor; Fluorescence; Gold nanoclusters; Off on off