title: Role of cardiotrophin-1 in the regulation of metabolic circadian rhythms and adipose core clock genes in mice and characterization of 24-h circulating CT-1 profiles in normal-weight and overweight/obese subjects.
abstract: Cardiotrophin (CT)-1 is a regulator of glucose and lipid homeostasis. In the present study, we analyzed whether CT-1 also acts to peripherally regulate metabolic rhythms and adipose tissue core clock genes in mice. Moreover, the circadian pattern of plasma CT-1 levels was evaluated in normal-weight and overweight subjects. The circadian rhythmicity of oxygen consumption rate (Vo2) was disrupted in aged obese CT-1-deficient (CT-1-/-) mice (12 mo). Although circadian rhythms ofVo2were conserved in young lean CT-1-/-mice (2 mo), CT-1 deficiency caused a phase shift of the acrophase. Most of the clock genes studied (Clock,Bmal1, andPer2)displayed a circadian rhythm in adipose tissue of both wild-type (WT) and CT-1-/-mice. However, the pattern was altered in CT-1-/-mice toward a lower percentage of the rhythm or lower amplitude, especially forBmal1andClock.Moreover, CT-1 mRNA levels in adipose tissue showed significant circadian fluctuations in young WT mice. In humans, CT-1 plasma profile exhibited a 24-h circadian rhythm in normal-weight but not in overweight subjects. The 24-h pattern of CT-1 was characterized by a pronounced increase during the night (from 02:00 to 08:00). These observations suggest a potential role for CT-1 in the regulation of metabolic circadian rhythms.-López-Yoldi, M., Stanhope, K. L., Garaulet, M., Chen, X. G., Marcos-Gómez, B., Carrasco-Benso, M. P., Santa Maria, E. M., Escoté, X., Lee, V., Nunez, M. V., Medici, V., Martínez-Ansó, E., Sáinz, N., Huerta, A. E., Laiglesia, L. M., Prieto, J., Martínez, J. A., Bustos, M., Havel, P. J., Moreno-Aliaga, M. J. Role of cardiotrophin-1 in the regulation of metabolic circadian rhythms and adipose core clock genes in mice and characterization of 24-h circulating CT-1 profiles in normal-weight and overweight/obese subjects.
authors.affiliation: Department of Nutrition, Food Science, and Physiology, University of Navarra, Pamplona, Spain.|Centre for Nutrition Research, University of Navarra, Pamplona, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: López-Yoldi
authors.affiliation: Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, Davis, California, USA.
authors.forename: Kimber L
authors.initials: KL
authors.lastname: Stanhope
authors.affiliation: Department of Physiology, Faculty of Biology, University of Murcia, Murcia, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Garaulet
authors.affiliation: Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, Davis, California, USA.
authors.forename: X Guoxia
authors.initials: XG
authors.lastname: Chen
authors.affiliation: Department of Nutrition, Food Science, and Physiology, University of Navarra, Pamplona, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Marcos-Gómez
authors.affiliation: Department of Physiology, Faculty of Biology, University of Murcia, Murcia, Spain.
authors.forename: María Paz
authors.initials: MP
authors.lastname: Carrasco-Benso
authors.affiliation: Biomedical Research Center for Hepatic and Digestive Diseases (CIBERehd) and Institute of Health Carlos III, Madrid, Spain.
authors.forename: Eva M
authors.initials: EM
authors.lastname: Santa Maria
authors.affiliation: Department of Nutrition, Food Science, and Physiology, University of Navarra, Pamplona, Spain.|Centre for Nutrition Research, University of Navarra, Pamplona, Spain.
authors.forename: Xavier
authors.initials: X
authors.lastname: Escoté
authors.affiliation: Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, Davis, California, USA.
authors.forename: Vivien
authors.initials: V
authors.lastname: Lee
authors.affiliation: Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, Davis, California, USA.
authors.forename: Marinelle V
authors.initials: MV
authors.lastname: Nunez
authors.affiliation: Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, Davis, California, USA.
authors.forename: Valentina
authors.initials: V
authors.lastname: Medici
authors.affiliation: Department of Hepatology and Gene Therapy, Center for Applied Medical Research (CIMA), University of Navarra, Pamplona, Spain.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Martínez-Ansó
authors.affiliation: Department of Nutrition, Food Science, and Physiology, University of Navarra, Pamplona, Spain.|Centre for Nutrition Research, University of Navarra, Pamplona, Spain.
authors.forename: Neira
authors.initials: N
authors.lastname: Sáinz
authors.affiliation: Department of Nutrition, Food Science, and Physiology, University of Navarra, Pamplona, Spain.|Centre for Nutrition Research, University of Navarra, Pamplona, Spain.
authors.forename: Ana E
authors.initials: AE
authors.lastname: Huerta
authors.affiliation: Department of Nutrition, Food Science, and Physiology, University of Navarra, Pamplona, Spain.|Centre for Nutrition Research, University of Navarra, Pamplona, Spain.
authors.forename: Laura M
authors.initials: LM
authors.lastname: Laiglesia
authors.affiliation: Biomedical Research Center for Hepatic and Digestive Diseases (CIBERehd) and Institute of Health Carlos III, Madrid, Spain.|Department of Hepatology and Gene Therapy, Center for Applied Medical Research (CIMA), University of Navarra, Pamplona, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Prieto
authors.affiliation: Department of Nutrition, Food Science, and Physiology, University of Navarra, Pamplona, Spain.|Centre for Nutrition Research, University of Navarra, Pamplona, Spain.|Biomedical Research Centre in Physiopathology of Obesity and Nutrition (CIBERobn), Institute of Health Carlos III, Madrid, Spain; and.|Navarra Institute for Health Research (IdiSNA), Pamplona, Spain.
authors.forename: J Alfredo
authors.initials: JA
authors.lastname: Martínez
authors.affiliation: Department of Hepatology and Gene Therapy, Center for Applied Medical Research (CIMA), University of Navarra, Pamplona, Spain.
authors.forename: Matilde
authors.initials: M
authors.lastname: Bustos
authors.affiliation: Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, Davis, California, USA.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Havel
authors.affiliation: Department of Nutrition, Food Science, and Physiology, University of Navarra, Pamplona, Spain; mjmoreno@unav.es.|Centre for Nutrition Research, University of Navarra, Pamplona, Spain.|Biomedical Research Centre in Physiopathology of Obesity and Nutrition (CIBERobn), Institute of Health Carlos III, Madrid, Spain; and.|Navarra Institute for Health Research (IdiSNA), Pamplona, Spain.
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Moreno-Aliaga
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism* / Q000502:physiology; D000293:Adolescent; D000328:Adult; D000818:Animals; D056926:CLOCK Proteins / Q000235:genetics* / Q000378:metabolism; D002940:Circadian Rhythm; D016207:Cytokines / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000097:blood / Q000378:metabolism*; D010101:Oxygen Consumption
chemical_list: D016207:Cytokines; C092073:cardiotrophin 1; D056926:CLOCK Proteins
keywords: Bmal1; Cry1; Per2; adipose tissue; obesity