title: Maternal Hypermethioninemia Affects Neurons Number, Neurotrophins Levels, Energy Metabolism, and Na+,K+-ATPase Expression/Content in Brain of Rat Offspring.
abstract: In the current study, we verified the effects of maternal hypermethioninemia on the number of neurons, apoptosis, nerve growth factor, and brain-derived neurotrophic factor levels, energy metabolism parameters (succinate dehydrogenase, complex II, and cytochrome c oxidase), expression and immunocontent of Na+,K+-ATPase, edema formation, inflammatory markers (tumor necrosis factor-alpha and interleukin-6), and mitochondrial hydrogen peroxide levels in the encephalon from the offspring. Pregnant Wistar rats were divided into two groups: the first one received saline (control) and the second group received 2.68 μmol methionine/g body weight by subcutaneous injections twice a day during gestation (approximately 21 days). After parturition, pups were killed at the 21st day of life for removal of encephalon. Neuronal staining (anti-NeuN) revealed a reduction in number of neurons, which was associated to decreased nerve growth factor and brain-derived neurotrophic factor levels. Maternal hypermethioninemia also reduced succinate dehydrogenase and complex II activities and increased expression and immunocontent of Na+,K+-ATPase alpha subunits. These results indicate that maternal hypermethioninemia may be a predisposing factor for damage to the brain during the intrauterine life.
authors.affiliation: Postgraduate Program in Biological Sciences-Biochemistry, Department of Biochemistry, Institute of Health Basic Sciences, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP, Porto Alegre, RS, 90035-003, Brazil.
authors.forename: Bruna M
authors.initials: BM
authors.lastname: Schweinberger
authors.affiliation: Postgraduate Program in Biological Sciences-Biochemistry, Department of Biochemistry, Institute of Health Basic Sciences, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP, Porto Alegre, RS, 90035-003, Brazil.
authors.forename: André F
authors.initials: AF
authors.lastname: Rodrigues
authors.affiliation: Postgraduate Program in Biological Sciences-Biochemistry, Department of Biochemistry, Institute of Health Basic Sciences, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP, Porto Alegre, RS, 90035-003, Brazil.
authors.forename: Elias
authors.initials: E
authors.lastname: Turcatel
authors.affiliation: Postgraduate Program in Biological Sciences-Biochemistry, Department of Biochemistry, Institute of Health Basic Sciences, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP, Porto Alegre, RS, 90035-003, Brazil.
authors.forename: Paula
authors.initials: P
authors.lastname: Pierozan
authors.affiliation: Postgraduate Program in Biological Sciences-Biochemistry, Department of Biochemistry, Institute of Health Basic Sciences, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP, Porto Alegre, RS, 90035-003, Brazil.
authors.forename: Leticia F
authors.initials: LF
authors.lastname: Pettenuzzo
authors.affiliation: Postgraduate Program in Biological Sciences-Biochemistry, Department of Biochemistry, Institute of Health Basic Sciences, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP, Porto Alegre, RS, 90035-003, Brazil.
authors.forename: Mateus
authors.initials: M
authors.lastname: Grings
authors.affiliation: Postgraduate program in Health Sciences, Laboratory of Bioenergetics, University of Southern Santa Catarina, Criciúma, SC, Brazil.
authors.forename: Giselli
authors.initials: G
authors.lastname: Scaini
authors.affiliation: Postgraduate Program in Biological Sciences-Biochemistry, Department of Biochemistry, Institute of Health Basic Sciences, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP, Porto Alegre, RS, 90035-003, Brazil.
authors.forename: Mariana M
authors.initials: MM
authors.lastname: Parisi
authors.affiliation: Postgraduate Program in Biological Sciences-Biochemistry, Department of Biochemistry, Institute of Health Basic Sciences, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP, Porto Alegre, RS, 90035-003, Brazil.
authors.forename: Guilhian
authors.initials: G
authors.lastname: Leipnitz
authors.affiliation: Postgraduate program in Health Sciences, Laboratory of Bioenergetics, University of Southern Santa Catarina, Criciúma, SC, Brazil.
authors.forename: Emilio L
authors.initials: EL
authors.lastname: Streck
authors.affiliation: Postgraduate Program in Biological Sciences-Biochemistry, Department of Biochemistry, Institute of Health Basic Sciences, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP, Porto Alegre, RS, 90035-003, Brazil.
authors.forename: Florencia M
authors.initials: FM
authors.lastname: Barbé-Tuana
authors.affiliation: Postgraduate Program in Biological Sciences-Biochemistry, Department of Biochemistry, Institute of Health Basic Sciences, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP, Porto Alegre, RS, 90035-003, Brazil. wyse@ufrgs.br.
authors.forename: Angela T S
authors.initials: ATS
authors.lastname: Wyse
mesh_terms: D000592:Amino Acid Metabolism, Inborn Errors / Q000139:chemically induced / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002452:Cell Count; D004734:Energy Metabolism / Q000502:physiology*; D005260:Female; D050938:Glycine N-Methyltransferase / Q000172:deficiency* / Q000378:metabolism; D008715:Methionine; D009414:Nerve Growth Factors / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D010084:Oxidation-Reduction; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
chemical_list: D009414:Nerve Growth Factors; D008715:Methionine; D050938:Glycine N-Methyltransferase; D000254:Sodium-Potassium-Exchanging ATPase
keywords: Brain; Energy metabolism; Maternal hypermethioninemia; Na+,K+-ATPase; Neurons number; Neurotrophins