title: Induction and suppression of type I interferon responses by mink enteritis virus in CRFK cells.
abstract: Mink enteritis virus (MEV) is one of the most important viral pathogens causing serious disease in mink. Type I interferon (IFN) plays a critical role in antiviral innate immunity and, for successful infection, many viruses have evolved evasive strategies against it. Here, we show that MEV infection does not evoke IFN or interferon-stimulated genes (ISGs) responses in feline kidney (CRFK) cells, and that MEV suppresses IFN production in both poly I:C-stimulated and untreated cells. In CRFK cells pre-exposure to IFN, show that infection with, and replication of, MEV remain unaffected. This inhibition appears to be mediated by the MEV nonstructural protein (NS1) with its ORI-binding domain playing a major role.
authors.affiliation: State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Jigui
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Mao
authors.affiliation: State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Ji
authors.initials: J
authors.lastname: Xi
authors.affiliation: State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Yongle
authors.initials: Y
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China. Electronic address: weiquan8@cau.edu.cn.
authors.forename: Weiquan
authors.initials: W
authors.lastname: Liu
mesh_terms: D000818:Animals; D002415:Cats; D002460:Cell Line; D005254:Feline Panleukopenia / Q000276:immunology*; D005786:Gene Expression Regulation / Q000187:drug effects; D007369:Interferon Inducers / Q000494:pharmacology; D007370:Interferon Type I / Q000276:immunology*; D053490:Mink enteritis virus / Q000502:physiology*; D011070:Poly I-C / Q000494:pharmacology; D017361:Viral Nonstructural Proteins / Q000378:metabolism; D014779:Virus Replication
chemical_list: D007369:Interferon Inducers; D007370:Interferon Type I; D017361:Viral Nonstructural Proteins; D011070:Poly I-C
keywords: Mink enteritis parvovirus; Nonstructural protein; ORI-binding domain; Type I interferon