title: Changes in Circulating B Cell Subsets Associated with Aging and Acute SIV Infection in Rhesus Macaques.
abstract: Aging and certain viral infections can negatively impact humoral responses in humans. To further develop the nonhuman primate (NHP) model for investigating B cell dynamics in human aging and infectious disease, a flow cytometric panel was developed to characterize circulating rhesus B cell subsets. Significant differences between human and macaque B cells included the proportions of cells within IgD+ and switched memory populations and a prominent CD21-CD27+ unswitched memory population detected only in macaques. We then utilized the expanded panel to analyze B cell alterations associated with aging and acute simian immunodeficiency virus (SIV) infection in the NHP model. In the aging study, distinct patterns of B cell subset frequencies were observed for macaques aged one to five years compared to those between ages 5 and 30 years. In the SIV infection study, B cell frequencies and absolute number were dramatically reduced following acute infection, but recovered within four weeks of infection. Thereafter, the frequencies of activated memory B cells progressively increased; these were significantly correlated with the magnitude of SIV-specific IgG responses, and coincided with impaired maturation of anti-SIV antibody avidity, as previously reported for HIV-1 infection. These observations further validate the NHP model for investigation of mechanisms responsible for B cells alterations associated with immunosenescence and infectious disease.
authors.affiliation: Center for Comparative Medicine, University of California Davis, Davis, California, United States of America.
authors.forename: W L William
authors.identifier: http://orcid.org/0000-0002-1258-7369
authors.initials: WL
authors.lastname: Chang
authors.affiliation: Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California Davis, Davis, California, United States of America.
authors.forename: Denise F
authors.initials: DF
authors.lastname: Gonzalez
authors.affiliation: Center for Comparative Medicine, University of California Davis, Davis, California, United States of America.|Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California Davis, Davis, California, United States of America.
authors.forename: Hung T
authors.initials: HT
authors.lastname: Kieu
authors.affiliation: Center for Comparative Medicine, University of California Davis, Davis, California, United States of America.
authors.forename: Luis D
authors.initials: LD
authors.lastname: Castillo
authors.affiliation: Department of Biomedical Sciences, School of Medicine, University of California Riverside, Riverside, California, United States of America.
authors.forename: Ilhem
authors.initials: I
authors.lastname: Messaoudi
authors.affiliation: Duke Human Vaccine Institute, Duke University Medical Center, Durham, North Carolina, United States of America.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Shen
authors.affiliation: Duke Human Vaccine Institute, Duke University Medical Center, Durham, North Carolina, United States of America.
authors.forename: Georgia D
authors.initials: GD
authors.lastname: Tomaras
authors.affiliation: Department of Medical Microbiology and Immunology, School of Medicine, University of California Davis, Davis, California, United States of America.|Division of Infectious Diseases, Department of Medicine, School of Medicine, University of California Davis, Davis, California, United States of America.
authors.forename: Barbara L
authors.initials: BL
authors.lastname: Shacklett
authors.affiliation: Center for Comparative Medicine, University of California Davis, Davis, California, United States of America.|Department of Pathology and Laboratory Medicine, School of Medicine, University of California Davis, Davis, California, United States of America.|California National Primate Research Center, University of California Davis, Davis, California, United States of America.
authors.forename: Peter A
authors.initials: PA
authors.lastname: Barry
authors.affiliation: Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California Davis, Davis, California, United States of America.
authors.forename: Ellen E
authors.initials: EE
authors.lastname: Sparger
mesh_terms: D000375:Aging / Q000097:blood* / Q000276:immunology*; D000818:Animals; D016175:B-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D005260:Female; D007156:Immunologic Memory / Q000276:immunology*; D008253:Macaca mulatta; D008297:Male; D016097:Simian Acquired Immunodeficiency Syndrome / Q000097:blood* / Q000276:immunology* / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D019562:Viral Load