title: Flow of essential elements in subcellular fractions during oxidative stress.
abstract: Essential trace elements are commonly found in altered concentrations in the brains of patients with neurodegenerative diseases. Many studies in trace metal determination and quantification are conducted in tissue, cell culture or whole brain. In the present investigation, we determined by ICP-MS Fe, Cu, Zn, Ca, Se, Co, Cr, Mg, and Mn in organelles (mitochondria, nuclei) and whole motor neuron cell cultured in vitro. We performed experiments using two ways to access oxidative stress: cell treatments with H2O2or Aβ-42 peptide in its oligomeric form. Both treatments caused accumulation of markers of oxidative stress, such as oxidized proteins and lipids, and alteration in DNA. Regarding trace elements, cells treated with H2O2showed higher levels of Zn and lower levels of Ca in nuclei when compared to control cells with no oxidative treatments. On the other hand, cells treated with Aβ-42 peptide in its oligomeric form showed higher levels of Mg, Ca, Fe and Zn in nuclei when compared to control cells. These differences showed that metal flux in cell organelles during an intrinsic external oxidative condition (H2O2treatment) are different from an intrinsic external neurodegenerative treatment.
authors.affiliation: Center for Natural Sciences and Humanities, Universidade Federal do ABC - UFABC, Santo André, SP, Brazil.
authors.forename: Larissa
authors.initials: L
authors.lastname: Lago
authors.affiliation: Center for Natural Sciences and Humanities, Universidade Federal do ABC - UFABC, Santo André, SP, Brazil.
authors.forename: Emilene A
authors.initials: EA
authors.lastname: Nunes
authors.affiliation: Center for Natural Sciences and Humanities, Universidade Federal do ABC - UFABC, Santo André, SP, Brazil.
authors.forename: Aryane A
authors.initials: AA
authors.lastname: Vigato
authors.affiliation: Department of Clinical Analysis, Toxicological and Bromatological, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo - USP, Ribeirão Prêto, Brazil.
authors.forename: Vanessa C O
authors.initials: VC
authors.lastname: Souza
authors.affiliation: Department of Clinical Analysis, Toxicological and Bromatological, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo - USP, Ribeirão Prêto, Brazil.
authors.forename: Fernando
authors.initials: F
authors.lastname: Barbosa
authors.affiliation: Center for Mathematics, Computation, and Cognition, Universidade Federal do ABC - UFABC, São Bernardo do Campo, SP, Brazil.
authors.forename: João R
authors.initials: JR
authors.lastname: Sato
authors.affiliation: Center for Natural Sciences and Humanities, Universidade Federal do ABC - UFABC, Santo André, SP, Brazil.
authors.forename: Bruno L
authors.initials: BL
authors.lastname: Batista
authors.affiliation: Center for Natural Sciences and Humanities, Universidade Federal do ABC - UFABC, Santo André, SP, Brazil. giselle.cerchiaro@ufabc.edu.br.
authors.forename: Giselle
authors.initials: G
authors.lastname: Cerchiaro
mesh_terms: D001923:Brain Chemistry; D002118:Calcium / Q000737:chemistry / Q000302:isolation & purification; D003300:Copper / Q000737:chemistry / Q000302:isolation & purification; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide; D007501:Iron / Q000737:chemistry / Q000302:isolation & purification; D008274:Magnesium / Q000737:chemistry / Q000302:isolation & purification; D008670:Metals / Q000737:chemistry / Q000302:isolation & purification*; D009046:Motor Neurons / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects; D013057:Spectrum Analysis; D013347:Subcellular Fractions; D014131:Trace Elements / Q000737:chemistry / Q000302:isolation & purification*; D015032:Zinc / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D008670:Metals; D014131:Trace Elements; D003300:Copper; D006861:Hydrogen Peroxide; D007501:Iron; D008274:Magnesium; D015032:Zinc; D002118:Calcium
keywords: Metal flow; Metal quantification; Neuroscience; Oxidative stress