title: The assembly and function of perinuclear actin cap in migrating cells.
abstract: Stress fibers are actin bundles encompassing actin filaments, actin-crosslinking, and actin-associated proteins that represent the major contractile system in the cell. Different types of stress fibers assemble in adherent cells, and they are central to diverse cellular processes including establishment of the cell shape, morphogenesis, cell polarization, and migration. Stress fibers display specific cellular organization and localization, with ventral fibers present at the basal side, and dorsal fibers and transverse actin arcs rising at the cell front from the ventral to the dorsal side and toward the nucleus. Perinuclear actin cap fibers are a specific subtype of stress fibers that rise from the leading edge above the nucleus and terminate at the cell rear forming a dome-like structure. Perinuclear actin cap fibers are fixed at three points: both ends are anchored in focal adhesions, while the central part is physically attached to the nucleus and nuclear lamina through the linker of nucleoskeleton and cytoskeleton (LINC) complex. Here, we discuss recent work that provides new insights into the mechanism of assembly and the function of these actin stress fibers that directly link extracellular matrix and focal adhesions with the nuclear envelope.
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 00, Prague, Czech Republic.
authors.forename: Miloslava
authors.initials: M
authors.lastname: Maninova
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 00, Prague, Czech Republic.
authors.forename: Josef
authors.initials: J
authors.lastname: Caslavsky
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 00, Prague, Czech Republic. vomy@biomed.cas.cz.
authors.forename: Tomas
authors.initials: T
authors.lastname: Vomastek
mesh_terms: D051344:Actin Capping Proteins / Q000378:metabolism*; D002465:Cell Movement / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism; D016764:Cell Polarity / Q000502:physiology; D048430:Cell Shape / Q000502:physiology*; D022001:Focal Adhesions / Q000502:physiology; D006801:Humans; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D009685:Nuclear Envelope / Q000378:metabolism; D022502:Stress Fibers / Q000502:physiology*
chemical_list: D051344:Actin Capping Proteins
keywords: Dorsal fibers; Focal adhesions; LINC; Perinuclear actin cap; Stress fibers; α-Actinin