title: Sensitization of Radioresistant Prostate Cancer Cells by Resveratrol Isolated from Arachis hypogaea Stems.
abstract: Resveratrol (RV, 3,4',5-trihydroxystilbene) is naturally produced by a wide variety of plants including grapes and peanuts (Arachis hypogaea). However, the yield of RV from peanut stem and its potential radiosensitizing effects in prostate cancer (PCa) have not been well investigated. In this study, we characterized RV in peanut stem extract (PSE) for the first time and showed that both RV and PSE dose-dependently induced cell death in DOC-2/DAB2 interactive protein (DAB2IP)-deficient PCa cells with the radioresistant phenotype. Furthermore, the combination of radiation with either RV or PSE induced the death of radioresistant PCa cells through delayed repair of radiation-induced DNA double-strand break (DSB) and prolonged G2/M arrest, which induced apoptosis. The administration of RV and PSE effectively enhanced radiation therapy in the shDAB2IP PCa xenograft mouse model. These results demonstrate the promising synergistic effect of RV and PSE combined with radiation in the treatment of radioresistant PCa.
authors.affiliation: Graduate Institute of Basic Medical Science, School of Medicine, China Medical University, Taichung, Taiwan.
authors.forename: Yu-An
authors.initials: YA
authors.lastname: Chen
authors.affiliation: Research Institute of Biotechnology, Hungkuang University, Taichung, Taiwan.
authors.forename: Hsiu-Man
authors.initials: HM
authors.lastname: Lien
authors.affiliation: Department of Microbiology and Immunology, Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
authors.forename: Min-Chuan
authors.initials: MC
authors.lastname: Kao
authors.affiliation: Department of Urology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America.
authors.forename: U-Ging
authors.initials: UG
authors.lastname: Lo
authors.affiliation: Department of Urology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America.|Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan.
authors.forename: Li-Chiung
authors.initials: LC
authors.lastname: Lin
authors.affiliation: Department of Urology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America.
authors.forename: Chun-Jung
authors.initials: CJ
authors.lastname: Lin
authors.affiliation: Department of Rehabilitation, Dachien General Hospital, Miaoli, Taiwan.
authors.forename: Sheau-Jiun
authors.initials: SJ
authors.lastname: Chang
authors.affiliation: School of Management, Feng Chia University, Taichung, Taiwan.
authors.forename: Chia-Chang
authors.initials: CC
authors.lastname: Chen
authors.affiliation: Department of Urology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America.
authors.forename: Jer-Tsong
authors.initials: JT
authors.lastname: Hsieh
authors.affiliation: Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan.
authors.forename: Ho
authors.initials: H
authors.lastname: Lin
authors.affiliation: Graduate Institute of Basic Medical Science, School of Medicine, China Medical University, Taichung, Taiwan.
authors.forename: Chih-Hsin
authors.initials: CH
authors.lastname: Tang
authors.affiliation: Graduate Institute of Basic Medical Science, School of Medicine, China Medical University, Taichung, Taiwan.|Department of Microbiology and Immunology, Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan.|Department of Nursing, Asia University, Taichung, Taiwan.|Molecular Infectious Disease Research Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
authors.forename: Chih-Ho
authors.initials: CH
authors.lastname: Lai
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D010367:Arachis / Q000737:chemistry*; D045744:Cell Line, Tumor; D059248:Chemoradiotherapy / Q000379:methods*; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D059566:M Phase Cell Cycle Checkpoints / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D018547:Plant Stems / Q000737:chemistry*; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D011836:Radiation Tolerance; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D023041:Xenograft Model Antitumor Assays
chemical_list: D013267:Stilbenes; D000077185:Resveratrol