title: Screening active compounds from Corydalis yanhusuo by combining high expression VEGF receptor HEK293 cell membrane chromatography with HPLC - ESI - IT - TOF - MSn method.
abstract: Corydalis Thizoma,or Yuanhu in China, is a common herbal drug used for thousands of years as analgesic in Chinese medicine that has been reported to have potential anti-angiogenic effects. In this study, a VEGFR/cell membrane chromatography (VEGFR/CMC) coupled with HPLC- ESI-IT-TOF-MSn system was developed and successfully applied for identifying active components from YuanHu extract acting on VEGFR. We identified tetrahydropalmatine and corydaline as bioactive components with VEGFR activity, thus confirming their inhibitory activity on VEGFR engineered HEK293 cell growth by MTT assay. The activity of tetrahydropalmatine and corydaline was compared with the positive control sorafenib in a range of concentration from 6.25 to 50.0μM, showing a dose-dependent inhibitory trend. These results indicate that the VEGFR/CMC coupled with HPLC- ESI-IT-TOF-MSn system can purify and identify specific bioactive components from complex systems, thus representing a promising tool for screening molecules active towards VEGFR from natural herbs.
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, 76# Yanta West Road, Xi'an, 710061, China; National-Provincial Joint Engineering Research Center for Natural Vascular Medicine Screening & Analysis, Xi'an 710061, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Wei
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, 76# Yanta West Road, Xi'an, 710061, China; National-Provincial Joint Engineering Research Center for Natural Vascular Medicine Screening & Analysis, Xi'an 710061, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Hu
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, 76# Yanta West Road, Xi'an, 710061, China; National-Provincial Joint Engineering Research Center for Natural Vascular Medicine Screening & Analysis, Xi'an 710061, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, 76# Yanta West Road, Xi'an, 710061, China; National-Provincial Joint Engineering Research Center for Natural Vascular Medicine Screening & Analysis, Xi'an 710061, China. Electronic address: slhan2008@mail.xjtu.edu.cn.
authors.forename: Shengli
authors.initials: S
authors.lastname: Han
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, 76# Yanta West Road, Xi'an, 710061, China; National-Provincial Joint Engineering Research Center for Natural Vascular Medicine Screening & Analysis, Xi'an 710061, China.
authors.forename: Sicen
authors.initials: S
authors.lastname: Wang
mesh_terms: D001600:Berberine Alkaloids / Q000302:isolation & purification* / Q000494:pharmacology; D002462:Cell Membrane / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031311:Corydalis / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D057809:HEK293 Cells; D006801:Humans; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014162:Transfection; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000037:antagonists & inhibitors* / Q000235:genetics
chemical_list: D001600:Berberine Alkaloids; C452799:corydaline; C014215:tetrahydropalmatine; D040301:Vascular Endothelial Growth Factor Receptor-2
keywords: Cell membrane chromatography; HPLC- ESI-IT-TOF-MSn; Vascular endothelial growth factor receptor; YuanHu