title: The effect of Isoprinosine treatment on persistent infection of Balb/c mice infected with murine gammaherpesvirus 68.
abstract: We demonstrated the positive effect of Isoprinosine treatment on persistent infection of Balb/c mice with murine gammaherpesvirus 68 (MHV-68). Increased number of leukocytes, increased percentage of neutrophils, elevated levels of virus-neutralizing (VN) antibodies, reduced number of atypical lymphocytes and reduced virus titers were detected in the examined organs after a 14-day treatment. The positive effect of Isoprinosine therapy vanished after 120-150 days. After this interval, we demonstrated lower numbers of leukocytes, lower levels of VN antibodies and an increased number of atypical lymphoid monocytes in the Isoprinosine-treated group. Immunological parameters correlated with increased titers of virus in all investigated organs. Evidence of immunostimulation was demonstrated by lower incidence of tumor formation (7.5%) in the group of MHV-infected and Isoprinosine-treated mice in comparison to group without Isoprinosine treatment (17.5%). The presented results showed that Isoprinosine therapy had a positive impact on persistent infection of mice with MHV-68, but this effect was time-limited. The improvement of the investigated parameters lasted for five months only. Our presented results confirmed that each treatment with Isoprinosine should be repeated and must be long-term in some chronic infections.
authors.forename: O
authors.initials: O
authors.lastname: Janíčková
authors.forename: L
authors.initials: L
authors.lastname: Ančicová
authors.forename: K
authors.initials: K
authors.lastname: Briestenská
authors.forename: J
authors.initials: J
authors.lastname: Mistríková
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000627:therapeutic use*; D002522:Chlorocebus aethiops; D018142:Gammaherpesvirinae; D006566:Herpesviridae Infections / Q000188:drug therapy*; D007542:Inosine Pranobex / Q000627:therapeutic use*; D017737:Macrophages, Peritoneal / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D041681:NIH 3T3 Cells; D014709:Vero Cells
chemical_list: D000998:Antiviral Agents; D007542:Inosine Pranobex
keywords: MHV-68; Isoprinosine; immunity; reactivation of latent virus; tumors.