title: Presence of Borrelia turdi and Borrelia valaisiana (Spirochaetales: Spirochaetaceae) in Ticks Removed From Birds in the North of Spain, 2009-2011.
abstract: The genus Borrelia includes species responsible for severe human diseases such as Lyme disease. Birds are involved in their epidemiology as dispersers of infected ticks (Ixodida: Ixodidae) and as reservoirs or amplifiers of the bacterium. Herein, the presence of Borrelia burgdorferi sensu lato (s.l.) Johnson, Schmid, Hyde, Steigerwalt & Brenner in 336 ticks collected from birds in the north of Spain from 2009 to 2011 was investigated. Nucleic acid extracts from 174 Ixodes frontalis (Panzer), 108 Haemaphysalis punctata Canestrini & Fanzango, 34 Hyalomma marginatum Koch, 17 Ixodes ricinus (L.), and 3 Ixodes spp. were screened for the presence of B. burgdorferi s.l. by PCR. Borrelia turdi was detected in 22 I. frontalis, 2 H. punctata, and 2 I. ricinus Additionally, 1 I. frontalis and 1 H. punctata were found to be infected with the human pathogen Borrelia valaisiana Moreover, 3 I. frontalis showed coinfection with both Borrelia species. This study corroborates the presence of B. turdi and B. valaisiana in ticks from birds in the north of Spain. The presence of these bacteria in larval specimens could suggest the role of birds as their reservoirs, or the occurrence of the cofeeding phenomenon. In addition, the detection of B. turdi and B. valaisiana in H. punctata and I. frontalis ticks, respectively, is reported for the first time.
authors.affiliation: Center of Rickettsiosis and Arthropod-Borne Diseases, Hospital San Pedro-CIBIR, Logroño, La Rioja, Spain (ampalomar@riojasalud.es; aportillo@riojasalud.es; psantibanez@riojasalud.es; jaoteo@riojasalud.es).
authors.forename: Ana M
authors.initials: AM
authors.lastname: Palomar
authors.affiliation: Center of Rickettsiosis and Arthropod-Borne Diseases, Hospital San Pedro-CIBIR, Logroño, La Rioja, Spain (ampalomar@riojasalud.es; aportillo@riojasalud.es; psantibanez@riojasalud.es; jaoteo@riojasalud.es).
authors.forename: Aránzazu
authors.initials: A
authors.lastname: Portillo
authors.affiliation: Center of Rickettsiosis and Arthropod-Borne Diseases, Hospital San Pedro-CIBIR, Logroño, La Rioja, Spain (ampalomar@riojasalud.es; aportillo@riojasalud.es; psantibanez@riojasalud.es; jaoteo@riojasalud.es).
authors.forename: Paula
authors.initials: P
authors.lastname: Santibáñez
authors.affiliation: Abies, Environment Resources Inc., Logroño, La Rioja, Spain (david@abies-sl.es; lidiaroncero@hotmail.com).
authors.forename: David
authors.initials: D
authors.lastname: Mazuelas
authors.affiliation: Abies, Environment Resources Inc., Logroño, La Rioja, Spain (david@abies-sl.es; lidiaroncero@hotmail.com).
authors.forename: Lidia
authors.initials: L
authors.lastname: Roncero
authors.affiliation: Aranzadi Society of Sciences, San Sebastián, Guipúzcoa, Spain (ogutierrezjimenez@yahoo.es).
authors.forename: Óscar
authors.initials: Ó
authors.lastname: Gutiérrez
authors.affiliation: Center of Rickettsiosis and Arthropod-Borne Diseases, Hospital San Pedro-CIBIR, Logroño, La Rioja, Spain (ampalomar@riojasalud.es; aportillo@riojasalud.es; psantibanez@riojasalud.es; jaoteo@riojasalud.es) jaoteo@riojasalud.es.
authors.forename: José A
authors.initials: JA
authors.lastname: Oteo
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D015748:Borrelia burgdorferi Group / Q000302:isolation & purification*; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005260:Female; D018884:Ixodes / Q000254:growth & development / Q000382:microbiology* / Q000502:physiology*; D007814:Larva / Q000254:growth & development / Q000382:microbiology / Q000502:physiology; D008193:Lyme Disease / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D008297:Male; D009758:Nymph / Q000254:growth & development / Q000382:microbiology / Q000502:physiology; D017422:Sequence Analysis, DNA / Q000662:veterinary; D020308:Songbirds; D013030:Spain / Q000453:epidemiology; D013984:Tick Infestations / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*
chemical_list: D001426:Bacterial Proteins; D021903:DNA, Ribosomal Spacer
keywords: Borrelia turdi, Borrelia valaisiana; Spain; bird; tick