title: Leaf-disc grafting for the transmission ofCandidatusLiberibacter asiaticus in citrus (Citrus sinensis; Rutaceae) seedlings.
abstract: OBJECTIVE
  The search for resistance/tolerance to the devastating citrus huanglongbing disease (syn. HLB or citrus greening) is generating an increasing number of new plants of diverse genetic makeup. As the increasing number of new plants require more space, resources, and time, the need for faster and more efficient HLB screening tests becomes crucial.
  
  
  RESULTS
  The leaf-disc grafting system described here consists in replacing a disc of leaf tissue with a similar disc from an infected plant. This can be performed in young seedlings not yet big enough to endure other types of grafting. Graft success and infection rates average approximately 80%.
  
  
  CONCLUSIONS
  We describe the successful adaptation of leaf-disc grafting as a powerful screening tool for HLB. The system requires minimal plant material and can be performed in seedlings at a very young age with increased efficiency in terms of time, space, and resources.
authors.affiliation: Citrus Research and Education Center, University of Florida, 700 Experiment Station Road, Lake Alfred, Florida 33850 USA.
authors.forename: Flavia
authors.initials: F
authors.lastname: Tabay Zambon
authors.affiliation: Citrus Research and Education Center, University of Florida, 700 Experiment Station Road, Lake Alfred, Florida 33850 USA.
authors.forename: Karen
authors.initials: K
authors.lastname: Plant
authors.affiliation: Citrus Research and Education Center, University of Florida, 700 Experiment Station Road, Lake Alfred, Florida 33850 USA.
authors.forename: Ed
authors.initials: E
authors.lastname: Etxeberria
keywords: citrus greening; disease transmission; grafting efficiency