title: Development of a duplex PCR for rapid detection and differentiation of Erysipelothrix rhusiopathiae vaccine strains and wild type strains.
abstract: The differentiation of vaccine strains from wild type strains is important for disease control. A duplex PCR for rapid detection and differentiation of Erysipelothrix rhusiopathiae vaccine strains and wild type strains was developed based on the DNA polymerase IV gene. This duplex PCR was sensitive and specific. The detection results were coincident with that of a single nucleotide polymorphisms based PCR but the detection process was more rapid. In conclusion, this duplex PCR was a useful tool for Erysipelothrix rhusiopathiae infections' differential diagnosis in China.
authors.affiliation: Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wu
authors.affiliation: Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Kang
authors.affiliation: Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
authors.forename: Chengzhi
authors.initials: C
authors.lastname: Cai
authors.affiliation: Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, China,; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan, China; Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China. Electronic address: jml8328@126.com.
authors.forename: Meilin
authors.initials: M
authors.lastname: Jin
mesh_terms: D001428:Bacterial Vaccines / Q000382:microbiology; D002681:China; D019951:DNA Polymerase beta / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004888:Erysipelothrix / Q000145:classification* / Q000235:genetics*; D004889:Erysipelothrix Infections / Q000175:diagnosis / Q000382:microbiology*; D058889:Molecular Typing / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D012680:Sensitivity and Specificity
chemical_list: D001428:Bacterial Vaccines; D004269:DNA, Bacterial; D019951:DNA Polymerase beta
keywords: Duplex PCR; Erysipelothrix rhusiopathiae; Rapid differential detection; Vaccine