title: PRC2 Represses Hormone-Induced Somatic Embryogenesis in Vegetative Tissue of Arabidopsis thaliana.
abstract: Many plant cells can be reprogrammed into a pluripotent state that allows ectopic organ development. Inducing totipotent states to stimulate somatic embryo (SE) development is, however, challenging due to insufficient understanding of molecular barriers that prevent somatic cell dedifferentiation. Here we show that Polycomb repressive complex 2 (PRC2)-activity imposes a barrier to hormone-mediated transcriptional reprogramming towards somatic embryogenesis in vegetative tissue of Arabidopsis thaliana. We identify factors that enable SE development in PRC2-depleted shoot and root tissue and demonstrate that the establishment of embryogenic potential is marked by ectopic co-activation of crucial developmental regulators that specify shoot, root and embryo identity. Using inducible activation of PRC2 in PRC2-depleted cells, we demonstrate that transient reduction of PRC2 activity is sufficient for SE formation. We suggest that modulation of PRC2 activity in plant vegetative tissue combined with targeted activation of developmental pathways will open possibilities for novel approaches to cell reprogramming.
authors.affiliation: Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, Uppsala, Sweden.|Institute of Microbiology, Centre Algatech, Opatovický mlýn, Třeboň, Czech Republic.
authors.forename: Iva
authors.initials: I
authors.lastname: Mozgová
authors.affiliation: Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, Uppsala, Sweden.
authors.forename: Rafael
authors.identifier: 0000-0002-1363-6978
authors.initials: R
authors.lastname: Muñoz-Viana
authors.affiliation: Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, Uppsala, Sweden.
authors.forename: Lars
authors.identifier: 0000-0002-6645-1862
authors.initials: L
authors.lastname: Hennig
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D007210:Indoleacetic Acids / Q000494:pharmacology; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism; D018520:Plant Shoots / Q000254:growth & development / Q000378:metabolism; D058439:Plant Somatic Embryogenesis Techniques; D063151:Polycomb Repressive Complex 2; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; C574170:PRC2 protein, Arabidopsis; D012097:Repressor Proteins; D063151:Polycomb Repressive Complex 2