title: Deep sequencing analysis reveals a TMV mutant with a poly(A) tract reduces host defense responses in Nicotiana benthamiana.
abstract: Tobacco mosaic virus (TMV) possesses an upstream pseudoknotted domain (UPD), which is important for replication. After substituting the UPD with an internal poly(A) tract (43 nt), a mutant TMV-43A was constructed. TMV-43A replicated slower than TMV and induced a non-lethal mosaic symptom in Nicotiana benthamiana. In this study, deep sequencing was performed to detect the differences of small RNA profiles between TMV- and TMV-43A-infected N. benthamiana. The results showed that TMV-43A produced lesser amount of virus-derived interfering RNAs (vsiRNAs) than that of TMV. However, the distributions of vsiRNAs generation hotspots between TMV and TMV-43A were similar. Expression of genes related to small RNA biogenesis in TMV-43A-infected N. benthamiana was significantly lower than that of TMV, which leads to generation of lesser vsiRNAs. The expressions of host defense response genes were up-regulated after TMV infection, as compared to TMV-43A-infected plants. Host defense response to TMV-43A infection was lower than that to TMV. The absence of UPD might contribute to the reduced host response to TMV-43A. Our study provides valuable information in the role of the UPD in eliciting host response genes after TMV infection in N. benthamiana. (187 words).
authors.affiliation: Department of Biological Sciences, National University of Singapore, Republic of Singapore.
authors.forename: Song
authors.initials: S
authors.lastname: Guo
authors.affiliation: Department of Biological Sciences, National University of Singapore, Republic of Singapore; Temasek Life Sciences Laboratory, Singapore, Republic of Singapore; National University of Singapore Research Institute in Suzhou, Jiangsu, People's Republic of China. Electronic address: dbswsm@nus.edu.sg.
authors.forename: Sek-Man
authors.initials: SM
authors.lastname: Wong
mesh_terms: D001482:Base Composition; D019295:Computational Biology / Q000379:methods; D018506:Gene Expression Regulation, Plant; D015967:Gene Expression Regulation, Viral; D023061:Gene Order; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D054884:Host-Pathogen Interactions; D009154:Mutation; D010935:Plant Diseases / Q000235:genetics / Q000821:virology; D011061:Poly A; D034622:RNA Interference; D034741:RNA, Small Interfering; D012367:RNA, Viral; D014026:Nicotiana / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; D012367:RNA, Viral; D011061:Poly A
keywords: Host defense response; TMV-43A; Tobacco mosaic virus (TMV); Virus-derived small interfering RNA