title: Transient expression of recombinant tissue plasminogen activator (rt-PA) gene in cucurbit plants using viral vector.
abstract: OBJECTIVE
  To use a transient expression system to express a truncated human tissue plasminogen activator (K2S) gene in cucurbit plants.
  
  
  RESULTS
  The recombinant tissue plasminogen activator protein (K2S form) was expressed in active form in cucurbit plants. Its molecular weight was 43 kDa. The plant-derived rt-PA was determined using goat anti-rabbit antibody by western blotting. Among the infected lines, the highest expression of rt-PA was 62 ng/100 mg per leaf tissue as measured by ELISA. The enzymatic activity of the plant-derived rt-PA was 0.8 IU/ml.
  
  
  CONCLUSIONS
  The K25 form of rt-PA was expressed for the first time using the viral expression system. Plant-derived rt-PA showed similar potency to commercially-available PA.
authors.affiliation: Department of Biotechnology, Ramin University of Agriculture and Natural Resources, Ahwaz, 63417-711, Iran.
authors.forename: Vahid Jalali
authors.initials: VJ
authors.lastname: Javaran
authors.affiliation: Department of Biotechnology, Ramin University of Agriculture and Natural Resources, Ahwaz, 63417-711, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Shafeinia
authors.affiliation: Department of Biotechnology, Tarbiat Modares University, Tehran, 14115-336, Iran. M_jalali@modares.ac.ir.
authors.forename: Mokhtar Jalali
authors.initials: MJ
authors.lastname: Javaran
authors.affiliation: Department of Biotechnology, Ramin University of Agriculture and Natural Resources, Ahwaz, 63417-711, Iran.
authors.forename: Esmaeil Ghasemi
authors.initials: EG
authors.lastname: Gojani
authors.affiliation: Department of Biotechnology, Tarbiat Modares University, Tehran, 14115-336, Iran.
authors.forename: Malihe
authors.initials: M
authors.lastname: Mirzaee
mesh_terms: D000906:Antibodies / Q000276:immunology; D028464:Cucurbita / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D008970:Molecular Weight; D009029:Mosaic Viruses; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000906:Antibodies; D011994:Recombinant Proteins; D010959:Tissue Plasminogen Activator
keywords: Cucurbit plant; Human tissue plasminogen activator (K2S); Molecular pharming; Transient gene expression