title: Differential dehydration effects on globular proteins and intrinsically disordered proteins during film formation.
abstract: Globular proteins composed of different secondary structures and fold types were examined by synchrotron radiation circular dichroism spectroscopy to determine the effects of dehydration on their secondary structures. They exhibited only minor changes upon removal of bulk water during film formation, contrary to previously reported studies of proteins dehydrated by lyophilization (where substantial loss of helical structure and gain in sheet structure was detected). This near lack of conformational change observed for globular proteins contrasts with intrinsically disordered proteins (IDPs) dried in the same manner: the IDPs, which have almost completely unordered structures in solution, exhibited increased amounts of regular (mostly helical) secondary structures when dehydrated, suggesting formation of new intra-protein hydrogen bonds replacing solvent-protein hydrogen bonds, in a process which may mimic interactions that occur when IDPs bind to partner molecules. This study has thus shown that the secondary structures of globular and intrinsically disordered proteins behave very differently upon dehydration, and that films are a potentially useful format for examining dehydrated soluble proteins and assessing IDPs structures.
authors.affiliation: Institute of Structural and Molecular Biology, Birkbeck College, University of London, London, UK.|Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, Brazil.
authors.forename: Juliana Sakamoto
authors.initials: JS
authors.lastname: Yoneda
authors.affiliation: Institute of Structural and Molecular Biology, Birkbeck College, University of London, London, UK.
authors.forename: Andew J
authors.initials: AJ
authors.lastname: Miles
authors.affiliation: Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, Brazil.
authors.forename: Ana Paula Ulian
authors.initials: AP
authors.lastname: Araujo
authors.affiliation: Institute of Structural and Molecular Biology, Birkbeck College, University of London, London, UK.
authors.forename: B A
authors.initials: BA
authors.lastname: Wallace
mesh_terms: D002942:Circular Dichroism; D003890:Desiccation; D006860:Hydrogen Bonding; D064267:Intrinsically Disordered Proteins / Q000737:chemistry*; D017510:Protein Folding
chemical_list: D064267:Intrinsically Disordered Proteins
keywords: dehydration; folding; globular proteins; intrinsically disordered proteins; secondary structure; synchrotron radiation circular dichroism (SRCD) spectroscopy; unfolding