title: Comparative Transcriptome Analysis of Raccoon Dog Skin to Determine Melanin Content in Hair and Melanin Distribution in Skin.
abstract: The raccoon dog (Nyctereutes procyonoides) is an important canid fur-bearing animal species worldwide. Chinese raccoon dogs that present a white mutation, especially those with a white coat. Exploring melanin biosynthesis in the hair and skin of raccoon dogs is important for understanding the survival and evolutionary mechanisms of them. In this study, we measured the content of melanin in the hair of two types of raccoon dog and generated stained slices of skin tissue. The results indicated that melanin biosynthesis occurs in the wild-type (W) and white-type (B) raccoon dog skin, although less melanin is produced in B skin. We then sequenced the skin transcriptomes of W and B, compared the similarities and differences in expressed genes. A comparison of the gene expression showed 60 up-regulated genes and 127 down-regulated genes in B skin. We analyzed the unigenes and pathways related to the melanogenesis pathway and found that TYR, TYRP1, MC1R, SLC24a5, SLC45a2 and OCA2 were significantly down-regulated in B skin and these results were verified via qRT-PCR. We surmised that the phenotypic characteristics of the white mutation might be caused by the reduced expression of these genes and this finding provides new insights for future experiments in raccoon dogs.
authors.affiliation: Key Laboratory of Special Economic Animal Genetic Breeding and Reproduction, Ministry of Agriculture, State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Juye Street NO. 4899 130112, Changchun, China.|College of Life Science, Jilin Agricultural University, Xincheng Street NO. 2888 130118, Changchun, China.
authors.forename: Zhanyu
authors.initials: Z
authors.lastname: Du
authors.affiliation: Beijing Gene-Health Huachuang Biotech Co., Ltd, Xueqing Rode NO. 9 100089, Beijing, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Huang
authors.affiliation: Key Laboratory of Special Economic Animal Genetic Breeding and Reproduction, Ministry of Agriculture, State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Juye Street NO. 4899 130112, Changchun, China.
authors.forename: Jiaping
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Special Economic Animal Genetic Breeding and Reproduction, Ministry of Agriculture, State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Juye Street NO. 4899 130112, Changchun, China.
authors.forename: Xingchao
authors.initials: X
authors.lastname: Song
authors.affiliation: Key Laboratory of Special Economic Animal Genetic Breeding and Reproduction, Ministry of Agriculture, State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Juye Street NO. 4899 130112, Changchun, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Xing
authors.affiliation: Key Laboratory of Special Economic Animal Genetic Breeding and Reproduction, Ministry of Agriculture, State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Juye Street NO. 4899 130112, Changchun, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: College of Life Science, Jilin Agricultural University, Xincheng Street NO. 2888 130118, Changchun, China.
authors.forename: Linbo
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Special Economic Animal Genetic Breeding and Reproduction, Ministry of Agriculture, State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Juye Street NO. 4899 130112, Changchun, China.|Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation, Ministry of Agriculture (nzdsys2016-3), Yuangmingyuan West-Rode NO. 2 100193, Beijing, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Xu
mesh_terms: D000818:Animals; D015536:Down-Regulation; D020869:Gene Expression Profiling; D006197:Hair / Q000378:metabolism* / Q000473:pathology; D008543:Melanins / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D014442:Monophenol Monooxygenase / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D046031:Raccoon Dogs / Q000378:metabolism*; D012867:Skin / Q000378:metabolism* / Q000473:pathology; D059467:Transcriptome
chemical_list: D008543:Melanins; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; D014442:Monophenol Monooxygenase