title: Primate embryogenesis predicts the hallmarks of human naïve pluripotency.
abstract: Naïve pluripotent mouse embryonic stem cells (ESCs) resemble the preimplantation epiblast and efficiently contribute to chimaeras. Primate ESCs correspond to the postimplantation embryo and fail to resume development in chimaeric assays. Recent data suggest that human ESCs can be 'reset' to an earlier developmental stage, but their functional capacity remains ill defined. Here, we discuss how the naïve state is inherently linked to preimplantation epiblast identity in the embryo. We hypothesise that distinctive features of primate development provide stringent criteria to evaluate naïve pluripotency in human and other primate cells. Based on our hypothesis, we define 12 key hallmarks of naïve pluripotency, five of which are specific to primates. These hallmarks may serve as a functional framework to assess human naïve ESCs.
authors.affiliation: Wellcome Trust-Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK teb45@cam.ac.uk.
authors.forename: Thorsten
authors.identifier: 0000-0001-8703-8949
authors.initials: T
authors.lastname: Boroviak
authors.affiliation: Wellcome Trust-Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK.|Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 4BG, UK.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Nichols
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology; D002454:Cell Differentiation; D002478:Cells, Cultured; D002678:Chimera / Q000196:embryology; D004622:Embryo, Mammalian; D047108:Embryonic Development / Q000502:physiology*; D053595:Embryonic Stem Cells / Q000502:physiology; D005855:Germ Layers / Q000166:cytology; D006801:Humans; D051379:Mice; D039904:Pluripotent Stem Cells / Q000502:physiology*; D011323:Primates / Q000196:embryology*; D051381:Rats
keywords: Amnion; Epiblast; Extraembryonic; Naïve pluripotency; Postimplantation; Primate