title: High-resolution sub-cellular imaging by correlative NanoSIMS and electron microscopy of amiodarone internalisation by lung macrophages as evidence for drug-induced phospholipidosis.
abstract: Correlative NanoSIMS and EM imaging of amiodarone-treated macrophages shows the internalisation of the drug at a sub-cellular level and reveals its accumulation within the lysosomes, providing direct evidence for amiodarone-induced phospholipidosis. Chemical fixation using tannic acid effectively seals cellular membranes aiding intracellular retention of diffusible drugs.
authors.affiliation: Centre for Microscopy, Characterisation and Analysis, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia. Haibo.Jiang@uwa.edu.au.
authors.forename: Haibo
authors.initials: H
authors.lastname: Jiang
authors.affiliation: National Centre of Excellence in Mass Spectrometry Imaging (NiCE-MSI), National Physical Laboratory, Hampton Road, Teddington, Middlesex TW11 0LW, UK. Paulina.Rakowska@npl.co.uk.
authors.forename: Melissa K
authors.initials: MK
authors.lastname: Passarelli
authors.affiliation: Institute of Ophthalmology, University College London, Bath Street, London EC1V 9EL, UK.
authors.forename: Peter M G
authors.initials: PM
authors.lastname: Munro
authors.affiliation: Centre for Microscopy, Characterisation and Analysis, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia. Haibo.Jiang@uwa.edu.au.
authors.forename: Matt R
authors.initials: MR
authors.lastname: Kilburn
authors.affiliation: GlaxoSmithKline, Gunnels Wood Road, Stevenage, Hertfordshire SG1 2NY, UK.
authors.forename: Andrew
authors.initials: A
authors.lastname: West
authors.affiliation: GlaxoSmithKline, Gunnels Wood Road, Stevenage, Hertfordshire SG1 2NY, UK.
authors.forename: Colin T
authors.initials: CT
authors.lastname: Dollery
authors.affiliation: National Centre of Excellence in Mass Spectrometry Imaging (NiCE-MSI), National Physical Laboratory, Hampton Road, Teddington, Middlesex TW11 0LW, UK. Paulina.Rakowska@npl.co.uk.
authors.forename: Ian S
authors.initials: IS
authors.lastname: Gilmore
authors.affiliation: National Centre of Excellence in Mass Spectrometry Imaging (NiCE-MSI), National Physical Laboratory, Hampton Road, Teddington, Middlesex TW11 0LW, UK. Paulina.Rakowska@npl.co.uk.
authors.forename: Paulina D
authors.initials: PD
authors.lastname: Rakowska
mesh_terms: D000638:Amiodarone / Q000737:chemistry / Q000494:pharmacology*; D000889:Anti-Arrhythmia Agents / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008168:Lung / Q000166:cytology / Q000187:drug effects; D008247:Lysosomes / Q000737:chemistry / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects*; D008854:Microscopy, Electron; D036103:Nanotechnology; D010743:Phospholipids / Q000378:metabolism*; D018629:Spectrometry, Mass, Secondary Ion
chemical_list: D000889:Anti-Arrhythmia Agents; D010743:Phospholipids; D000638:Amiodarone