title: Development of a Topical Resveratrol Formulation for Commercial Applications Using Dendrimer Nanotechnology.
abstract: Resveratrol (RSV) is well known for its anti-oxidant and anti-aging properties. However, resveratrol is insoluble in water and has stability issues. Recently, efforts were placed to prepare a resveratrol-based advanced anti-aging topical product but it contains harsh organic solvents and oils that could be harmful to the human body and the environment. Hence, we propose the use of a multifunctional dendrimer to solve the solubility and stability issues of resveratrol. A dendrimer-resveratrol complex was prepared, optimized and tested for solubility enhancement, stability in solution and cream dosage forms. We have also developed a high performance liquid chromatography method to measure the resveratrol within the final product. PAMAM dendrimers increased the solubility and stability of resveratrol in water and semisolid dosage forms. Therefore, this product would be water based 'green' formulation devoid of harsh organic solvents and oils and can be safely applied to the skin. Additionally, we have shown that the dendrimer helped to increase overall RSV loading and skin penetration of resveratrol. The dendrimer-RSV formulation was successfully scaled up towards commercialization. Dendrimer with RSV has led to an innovation in anti-aging cream and solutions that could be commercially marketed. Dendrimer-RSV complex could also be added to other product forms for additional purposes and applications.
authors.affiliation: School of Pharmacy, Concordia University Wisconsin, 12800 N. Lake Shore Drive, Mequon, WI 53097, USA. Tyler.pentek@cuw.edu.
authors.forename: Tyler
authors.initials: T
authors.lastname: Pentek
authors.affiliation: School of Pharmacy, Concordia University Wisconsin, 12800 N. Lake Shore Drive, Mequon, WI 53097, USA. eric.newenhouse@cuw.edu.
authors.forename: Eric
authors.initials: E
authors.lastname: Newenhouse
authors.affiliation: School of Pharmacy, Concordia University Wisconsin, 12800 N. Lake Shore Drive, Mequon, WI 53097, USA. brennin.obrien@cuw.edu.
authors.forename: Brennin
authors.initials: B
authors.lastname: O'Brien
authors.affiliation: School of Pharmacy, Concordia University Wisconsin, 12800 N. Lake Shore Drive, Mequon, WI 53097, USA. abhay.chauhan@cuw.edu.|School of Pharmacy, Medical College of Wisconsin, Milwaukee, 8701 W. Watertown Plank Road, Milwaukee, WI 53226, USA. abhay.chauhan@cuw.edu.
authors.forename: Abhay Singh
authors.initials: AS
authors.lastname: Chauhan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000493:pharmacokinetics*; D050091:Dendrimers / Q000737:chemistry*; D015194:Diffusion Chambers, Culture; D004355:Drug Stability; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010539:Permeability; D011136:Polysorbates / Q000737:chemistry; D051381:Rats; D000077185:Resveratrol; D012867:Skin / Q000187:drug effects / Q000378:metabolism*; D063465:Skin Cream / Q000737:chemistry / Q000493:pharmacokinetics*; D012995:Solubility; D013267:Stilbenes / Q000737:chemistry / Q000493:pharmacokinetics*; D014867:Water / Q000737:chemistry
chemical_list: D000975:Antioxidants; D050091:Dendrimers; C104700:PAMAM Starburst; D011136:Polysorbates; D013267:Stilbenes; D014867:Water; D000077185:Resveratrol
keywords: PAMAM; antiaging; cream; dendrimer; resveratrol; safe; solubility; stability; topical; transdermal