title: Conformational Effects of UV Light on DNA Origami.
abstract: The responses of DNA origami conformation to UV radiation of different wavelengths and doses are investigated. Short- and medium-wavelength UV light can cause photo-lesions in DNA origami. At moderate doses, the lesions do not cause any visible defects in the origami, nor do they significantly affect the hybridization capability. Instead, they help relieve the internal stress in the origami structure and restore it to the designed conformation. At high doses, staple dissociation increases which causes structural disintegration. Long-wavelength UV does not show any effect on origami conformation by itself. We show that this UV range can be used in conjunction with photoactive molecules for photo-reconfiguration, while avoiding any damage to the DNA structures.
authors.affiliation: School of Mechanical Engineering, Purdue University , West Lafayette, Indiana 47907, United States.
authors.forename: Haorong
authors.identifier: 0000-0002-8218-9817
authors.initials: H
authors.lastname: Chen
authors.affiliation: School of Mechanical Engineering, Purdue University , West Lafayette, Indiana 47907, United States.
authors.forename: Ruixin
authors.identifier: 0000-0002-9079-1138
authors.initials: R
authors.lastname: Li
authors.affiliation: Department of Chemistry and Chemical Engineering, Chalmers University of Technology , SE-412 96 Gothenburg, Sweden.
authors.forename: Shiming
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Chemistry and Chemical Engineering, Chalmers University of Technology , SE-412 96 Gothenburg, Sweden.
authors.forename: Joakim
authors.initials: J
authors.lastname: Andréasson
authors.affiliation: School of Mechanical Engineering, Purdue University , West Lafayette, Indiana 47907, United States.
authors.forename: Jong Hyun
authors.identifier: 0000-0002-0507-3052
authors.initials: JH
authors.lastname: Choi
mesh_terms: D004247:DNA / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D014466:Ultraviolet Rays
chemical_list: D004247:DNA