title: Phenolic Composition and Antioxidant and Antiproliferative Activities of the Extracts of Twelve Common Bean (Phaseolus vulgarisL.) Endemic Ecotypes of Southern Italy before and after Cooking.
abstract: Beans are important dietary components with versatile health benefits. We analysed the extracts of twelve ecotypes ofPhaseolus vulgarisin order to determine their phenolic profiles, antioxidant activity, and thein vitroantiproliferative activity. Ultra-performance liquid chromatography with diode array detector (UPLC-DAD) admitted us to detect and quantify some known polyphenols, such as gallic acid, chlorogenic acid, epicatechin, myricetin, formononetin, caffeic acid, and kaempferol. The antioxidant activity (AA) ranged from 1.568 ± 0.041 to 66.572 ± 3.197 mg necessary to inhibit the activity of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical by 50% (EC50). The extracts, except those obtained from the nonpigmented samples, were capable of inhibiting the proliferation of the human epithelial colorectal adenocarcinoma (Caco-2) cells, human breast cancer cells MCF-7, and A549 NSCLC cell line. Cultivars differed in composition and concentration of polyphenols including anthocyanins; cooking affected the antioxidant activity only marginally. Qualitative and quantitative differences in phenolic composition between the groups of beans influenced the biological activities; on the other hand, we did not find significant differences on the biological activities within the same variety, before and after cooking.
authors.affiliation: Institute of Food Science, CNR-ISA, Via Roma 64, 83100, Avellino, Italy.
authors.forename: Maria Neve
authors.initials: MN
authors.lastname: Ombra
authors.affiliation: Institute of Food Science, CNR-ISA, Via Roma 64, 83100, Avellino, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: d'Acierno
authors.affiliation: Institute of Food Science, CNR-ISA, Via Roma 64, 83100, Avellino, Italy.
authors.forename: Filomena
authors.identifier: 0000-0002-5759-7499
authors.initials: F
authors.lastname: Nazzaro
authors.affiliation: Azienda "F. Marsocci", Via Varignano 7, 80011 Acerra, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Riccardi
authors.affiliation: Azienda "F. Marsocci", Via Varignano 7, 80011 Acerra, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Spigno
authors.affiliation: CREA, Via dei Cavalleggeri 25, 84098 Pontecagnano, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Zaccardelli
authors.affiliation: CREA, Via dei Cavalleggeri 25, 84098 Pontecagnano, Italy.
authors.forename: Catello
authors.initials: C
authors.lastname: Pane
authors.affiliation: Institute of Food Science, CNR-ISA, Via Roma 64, 83100, Avellino, Italy.
authors.forename: Mena
authors.initials: M
authors.lastname: Maione
authors.affiliation: Institute of Food Science, CNR-ISA, Via Roma 64, 83100, Avellino, Italy.
authors.forename: Florinda
authors.initials: F
authors.lastname: Fratianni
mesh_terms: D000072283:A549 Cells; D000872:Anthocyanins / Q000032:analysis; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D018938:Caco-2 Cells; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003296:Cooking; D060146:Ecotype; D005419:Flavonoids / Q000032:analysis; D006801:Humans; D007558:Italy; D061986:MCF-7 Cells; D027805:Phaseolus / Q000737:chemistry* / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D059808:Polyphenols / Q000032:analysis* / Q000494:pharmacology; D025341:Principal Component Analysis
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts; D059808:Polyphenols