title: Structure Characterization and Lead Detoxification Effect of Carboxymethylated Melanin Derived from Lachnum Sp.
abstract: In the present study, an intracellular melanin, named LIM205, was separated from Lachnum YM205 mycelia and was purified on a Sephadex G-15 column. The molecular weight of LIM205 was determined as 522 Da, and its molecular formula was speculated as C28H14N2O7S. The possible chemical structure of LIM205 was determined according to the results of Fourier transform infrared (FT-IR),1H NMR,13C NMR, and pyrolysis/GC-MS analysis. With the aim to increase its water solubility, its carboxymethylated derivative, named CLIM205, was formed by the substitution of hydrogen atoms in LIM205 with one, two, and three carboxymethylate groups. FT-IR, UV, and ESI-MS analysis demonstrated that the carboxymethylate groups were conjugated onto LIM205. The lead detoxification activities of LIM205 and CLIM205 had also been investigated. In vivo test showed that both LIM205 and CLIM205 reduced the tissue lead concentration, enhanced lead excretion, and reversed lead-induced alterations in superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) concentrations in mice, with CLIM205 showed better efficacy. The study indicates that LIM205 and CLIM205 have significant lead detoxification effect which will contribute to solve related problems.
authors.affiliation: College of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zong
authors.affiliation: College of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, China. lijinglei@hfut.edu.cn.
authors.forename: Jinglei
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, China.
authors.forename: Farnaz
authors.initials: F
authors.lastname: Shaikh
authors.affiliation: College of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.affiliation: College of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, China. yeming123@hfut.edu.cn.
authors.forename: Ming
authors.initials: M
authors.lastname: Ye
mesh_terms: D000818:Animals; D007854:Lead; D007855:Lead Poisoning / Q000188:drug therapy*; D008297:Male; D008543:Melanins / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D025282:Mycelium / Q000737:chemistry*; D020037:Sordariales / Q000737:chemistry*
chemical_list: D008543:Melanins; D007854:Lead
keywords: Carboxymethylated derivative; Lachnum melanin; Lead detoxification; Structure