title: Structural study of the effects of mutations in proteins to identify the molecular basis of the loss of local structural fluidity leading to the onset of autoimmune diseases.
abstract: Protein-Protein Interactions (PPIs) are crucial in most of the biological processes and PPI dysfunctions are known to be associated with the onsets of various diseases. One of such diseases is the auto-immune disease. Auto-immune diseases are one among the less studied group of diseases with very high mortality rates. Thus, we tried to correlate the appearances of mutations with their probable biochemical basis of the molecular mechanisms leading to the onset of the disease phenotypes. We compared the effects of the Single Amino Acid Variants (SAVs) in the wild type and mutated proteins to identify any structural deformities that might lead to altered PPIs leading ultimately to disease onset. For this we used Relative Solvent Accessibility (RSA) as a spatial parameter to compare the structural perturbation in mutated and wild type proteins. We observed that the mutations were capable to increase intra-chain PPIs whereas inter-chain PPIs would remain mostly unaltered. This might lead to more intra-molecular friction causing a deleterious alteration of protein's normal function. A Lyapunov exponent analysis, using the altered RSA values due to polymorphic and disease causing mutations, revealed polymorphic mutations have a positive mean value for the Lyapunov exponent while disease causing mutations have a negative mean value. Thus, local spatial stochasticity has been lost due to disease causing mutations, indicating a loss of structural fluidity. The amino acid conversion plot also showed a clear tendency of altered surface patch residue conversion propensity than polymorphic conversions. So far, this is the first report that compares the effects of different kinds of mutations (disease and non-disease causing polymorphic mutations) in the onset of autoimmune diseases.
authors.affiliation: Department of Biochemistry and Biophysics, University of Kalyani, Kalyani 741235, Nadia, West Bengal, India.
authors.forename: Ananya
authors.initials: A
authors.lastname: Ali
authors.affiliation: Department of Biochemistry and Biophysics, University of Kalyani, Kalyani 741235, Nadia, West Bengal, India.
authors.forename: Semanti
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Department of Biochemistry and Biophysics, University of Kalyani, Kalyani 741235, Nadia, West Bengal, India. Electronic address: angshu@klyuniv.ac.in.
authors.forename: Angshuman
authors.initials: A
authors.lastname: Bagchi
mesh_terms: D001327:Autoimmune Diseases / Q000209:etiology*; D006801:Humans; D009154:Mutation; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000235:genetics*
chemical_list: D011506:Proteins
keywords: Autoimmune diseases; Lyaunov exponent; Mutation; PPI; Relative solvent accessibility