title: Silicone rubber membrane with specific pore size enhances wound regeneration.
abstract: A porous structure is critically important for wound dressing or tissue engineering scaffolds. However, the influence of the pore sizes on cell proliferation, tissue regeneration and the underlying mechanism remains unclear. In this study, silicone rubber membranes with different pore sizes were prepared using certain constituents of liquid silicone rubber precursor/liquid paraffin/hexane based on our previous studies. It was found that pore size had a significant impact on cell proliferation and wound healing. The CCK8 analysis revealed that the membrane with a certain pore size (110.47 μm, middle pore membrane, MPM) was suitable for cell proliferation compared with the membranes with other pore sizes (218.03 μm, large pore membrane, LPM; 5.27 μm, small pore membrane, SPM; non-porous membrane, NPM). Further studies demonstrated that the MPM promoted cell proliferation via activating the Wnt/β-catenin signalling pathway. More importantly, wound healing experiments showed that 7 days post-wounding, the rate of wound healing was 89.25% with the MPM, which was significantly higher than with LPM, SPM or NPM. The in vivo data indicated that wound healing was accelerated by treatment with a silicone rubber membrane with a pore size of 110.47 μm. Our results strongly suggest that different pore structures might affect cell proliferation and wound healing and that a silicone rubber membrane with a specific pore size could potentially be used as a promising wound dressing. Copyright © 2017 John Wiley & Sons, Ltd.
authors.affiliation: Department of Neurology, Xinqiao Hospital & The Second Affiliated Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xu
authors.affiliation: Institute of Burn Research, Southwest Hospital, Third Military Medical University; State Key Laboratory of Trauma, Burn and Combined Injury; Chongqing Key Laboratory for Disease Proteomics, Chongqing, China.|Department of Otolaryngology, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Bai
authors.affiliation: State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, Chengdu, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, Chengdu, China.
authors.forename: Hesheng
authors.initials: H
authors.lastname: Xia
authors.affiliation: Institute of Burn Research, Southwest Hospital, Third Military Medical University; State Key Laboratory of Trauma, Burn and Combined Injury; Chongqing Key Laboratory for Disease Proteomics, Chongqing, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Kong
authors.affiliation: Institute of Burn Research, Southwest Hospital, Third Military Medical University; State Key Laboratory of Trauma, Burn and Combined Injury; Chongqing Key Laboratory for Disease Proteomics, Chongqing, China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Yao
authors.affiliation: Institute of Burn Research, Southwest Hospital, Third Military Medical University; State Key Laboratory of Trauma, Burn and Combined Injury; Chongqing Key Laboratory for Disease Proteomics, Chongqing, China.
authors.forename: Rongshuai
authors.initials: R
authors.lastname: Yan
authors.affiliation: Institute of Burn Research, Southwest Hospital, Third Military Medical University; State Key Laboratory of Trauma, Burn and Combined Injury; Chongqing Key Laboratory for Disease Proteomics, Chongqing, China.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute of Burn Research, Southwest Hospital, Third Military Medical University; State Key Laboratory of Trauma, Burn and Combined Injury; Chongqing Key Laboratory for Disease Proteomics, Chongqing, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Hu
authors.affiliation: Institute of Burn Research, Southwest Hospital, Third Military Medical University; State Key Laboratory of Trauma, Burn and Combined Injury; Chongqing Key Laboratory for Disease Proteomics, Chongqing, China.
authors.forename: Meixi
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Neurology, Xinqiao Hospital & The Second Affiliated Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Qingwu
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Institute of Burn Research, Southwest Hospital, Third Military Medical University; State Key Laboratory of Trauma, Burn and Combined Injury; Chongqing Key Laboratory for Disease Proteomics, Chongqing, China.
authors.forename: Gaoxing
authors.initials: G
authors.lastname: Luo
authors.affiliation: Institute of Burn Research, Southwest Hospital, Third Military Medical University; State Key Laboratory of Trauma, Burn and Combined Injury; Chongqing Key Laboratory for Disease Proteomics, Chongqing, China.
authors.forename: Jun
authors.identifier: 0000-0002-6014-3834
authors.initials: J
authors.lastname: Wu
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects / Q000235:genetics; D008567:Membranes, Artificial; D008807:Mice, Inbred BALB C; D016062:Porosity; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D062326:Re-Epithelialization / Q000187:drug effects / Q000235:genetics; D012038:Regeneration / Q000187:drug effects*; D012826:Silicone Elastomers / Q000494:pharmacology*; D060449:Wnt Signaling Pathway / Q000187:drug effects / Q000235:genetics; D014945:Wound Healing / Q000187:drug effects*; D051176:beta Catenin / Q000378:metabolism
chemical_list: D008567:Membranes, Artificial; D018809:Proliferating Cell Nuclear Antigen; D012826:Silicone Elastomers; D051176:beta Catenin
keywords: Wnt/β-catenin signalling pathway; cell proliferation; pore size; silicone rubber; wound healing