title: Entrapment efficiency of pyridoxine hydrochloride in unilamellar liposomes: experimental versus model-generated data.
abstract: The present study investigates the effect of the preparation method (four methods) and formulation additives (propylene glycol (PG) and cholesterol (CH)) on the entrapment efficiency (EE) of pyridoxine hydrochloride (vitamin B6 (VB6)), representing hydrophilic water-soluble low permeable vitamins, in unilamellar liposomes. The main aim is to compare determined EE with predicted values generated using a web-published, computational model. Results showed that among the different preparation methods, modified film hydration showed significantly higher EE (p < 0.05). With regard to formulation additives, PG (5% w/v) produced smaller vesicles size with narrow size distribution. Agreement between determined and model-generated EE values was more evident in formulae with narrow size distribution (polydispersity index (PdI) below 0.23). Formulae containing PG showed slightly higher determined than predicted EE values indicating vitamin-phospholipid bilayer interaction. Meanwhile, agreement between determined and predicted EE was limited to VB6-to-phospholipid ratio below (1.2:2). The comparison provided further insight into the usefulness of the prediction model factors affecting agreement between determined and predicted EE data.
authors.affiliation: a Department of Pharmaceutics , Faculty of Pharmacy, Damanhour University , Damanhour , Egypt and.|b Department of Pharmaceutics , Faculty of Pharmacy, Alexandria University , Alexandria , Egypt.
authors.forename: Heba
authors.initials: H
authors.lastname: Abd-El-Azim
authors.affiliation: b Department of Pharmaceutics , Faculty of Pharmacy, Alexandria University , Alexandria , Egypt.
authors.forename: Alyaa
authors.initials: A
authors.lastname: Ramadan
authors.affiliation: b Department of Pharmaceutics , Faculty of Pharmacy, Alexandria University , Alexandria , Egypt.
authors.forename: Noha
authors.initials: N
authors.lastname: Nafee
authors.affiliation: b Department of Pharmaceutics , Faculty of Pharmacy, Alexandria University , Alexandria , Egypt.
authors.forename: Nawal
authors.initials: N
authors.lastname: Khalafallah
mesh_terms: D002784:Cholesterol / Q000737:chemistry; D003198:Computer Simulation; D004339:Drug Compounding; D065546:Drug Liberation; D057927:Hydrophobic and Hydrophilic Interactions; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D019946:Propylene Glycol / Q000737:chemistry; D011736:Pyridoxine / Q000737:chemistry*; D013499:Surface Properties; D053835:Unilamellar Liposomes / Q000737:chemistry*; D014867:Water
chemical_list: D053835:Unilamellar Liposomes; D014867:Water; D019946:Propylene Glycol; D002784:Cholesterol; D011736:Pyridoxine
keywords: Computational model; entrapment efficiency; liposomes; vitamin B6; water-soluble vitamins