title: Monitoring immune response after allogeneic transplantation of mesenchymal stem cells for osteochondral repair.
abstract: The aim of this study was to investigate the safety of using allogeneic magnetically labelled mesenchymal stem cells (m-MSCs) to ameliorate osteochondral repair, with immune surveillance using a mixed lymphocyte reaction (MLR) assay. Twenty knees of Japanese white rabbits were randomly divided into two groups: the control (autologous) group, where 2 × 105autologous m-MSCs were transplanted into the defect site; the experimental (allogeneic) group, where 2 × 105m-MSCs from Dutch rabbits were transplanted into the defect of Japanese white rabbits. The rabbits were then euthanized after 12 weeks. The repaired tissue was stained with toluidine blue stain in order to produce histological scoring on the Fortier scale. Splenocytes were used to evaluate anti-donor alloreactivity by a MLR assay using the carboxyfluorescein diacetate succimidyl ester (CFSE) labelling technique (CFSE-MLR). In both groups, complete repair of the subchondral bone covered by a layer of chondrogenic tissue was confirmed. Also, there was no histologically significant difference on the Fortier scale. Using the CFSE-MLR assay, CD4+ T-cells showed alloreactivity in each combination, while the stimulation index of CD4+ T-cells was not statistically different between the control and experimental groups. Allogeneic m-MSCs are a safe alternative source to autologous m-MSCs, upholding repair of an osteochondral defect for clinical application using universal donor MSCs through a one-stage surgical procedure. Copyright © 2017 John Wiley & Sons, Ltd.
authors.affiliation: Department of Orthopaedic Surgery, Integrated Health Sciences, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.|Department of Surgery, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt.
authors.forename: Elhussein Elbadry
authors.identifier: 0000-0001-8877-8666
authors.initials: EE
authors.lastname: Mahmoud
authors.affiliation: Department of Gastroenterological and Transplant Surgery, Applied Life Sciences, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Tanaka
authors.affiliation: Department of Orthopaedic Surgery, Integrated Health Sciences, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.|Medical Center for Translational and Clinical Research, Hiroshima University Hospital, Hiroshima, Japan.
authors.forename: Naosuke
authors.initials: N
authors.lastname: Kamei
authors.affiliation: Department of Orthopaedic Surgery, Integrated Health Sciences, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Harada
authors.affiliation: Department of Gastroenterological and Transplant Surgery, Applied Life Sciences, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Ohdan
authors.affiliation: Department of Orthopaedic Surgery, Integrated Health Sciences, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Nobuo
authors.initials: N
authors.lastname: Adachi
authors.affiliation: Department of Orthopaedic Surgery, Integrated Health Sciences, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Ochi
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000378:metabolism; D020219:Chondrogenesis; D007109:Immunity; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis; D011817:Rabbits; D014184:Transplantation, Homologous; D014945:Wound Healing
keywords: CD4+ T-cells; CFSE-MLR assay; allogeneic m-MSCs; immune response; osteochondral repair