title: The SDF-1/CXCR4 axis promotes recovery after spinal cord injury by mediating bone marrow-derived from mesenchymal stem cells.
abstract: This study aims to explore the role of the SDF-1/CXCR4 axis in mediating BMSCs and SCI recovery. BMSCs were collected and SCI rat models were established. Wistar rats were assigned into the blank control, sham, SCI, SCI + BMSCs, SCI + BMSCs + SDF-1, SCI + BMSCs + AMD3100 (an inhibitor of SDF-1/CXCR4 axis) and SCI + BMSCs + SDF-1 + AMD3100 groups. Hind limb motor function was measured 7, 14, 21 and 28 days after operation. qRT-PCR, western blotting and ELISA was performed to determine the expressions of SDF-1, CXCR4, NGF, BDNF, GFAP and GAP-43, TNF-α, IL-1β, L-6 and IFN-γ. Hind limb motor function scores 7 days after the operation were reduced in the SCI rats of the blank control and sham groups. Hind limb function was found to be better in the SCI + BMSCs and SCI + BMSCs + SDF-1 groups than in the SCI, SCI + BMSCs + AMD3100 and SCI + BMSCs + SDF-1 + AMD3100 groups 14, 21 and 28 days after operation. Furthermore, the SCI group had lower SDF-1, CXCR4, NGF, BDNF and GAP-43 expressions but higher GFAP, TNF-α, IL-1β, IL-6 and IFN-γ than the blank control and sham groups 28 days after operation. While, the SCI + BMSCs, SCI + BMSCs + SDF-1 and SCI + BMSCs + SDF-1 + AMD3100 groups displayed opposite trends to the SCI and SCI + BMSCs + AMD3100 groups. In conclusion, SDF-1/CXCR4 axis promotes recovery after SCI by mediating BMSCs.
authors.affiliation: Department of Orthopedic, Huaihe Hospital of Henan University, Kaifeng 475000, P.R. China.
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Wang
authors.affiliation: Department of Orthopedic, Huaihe Hospital of Henan University, Kaifeng 475000, P.R. China.
authors.forename: Yi-Xun
authors.initials: YX
authors.lastname: Liu
authors.affiliation: Department of Orthopedic, Huaihe Hospital of Henan University, Kaifeng 475000, P.R. China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Orthopedic, Huaihe Hospital of Henan University, Kaifeng 475000, P.R. China.
authors.forename: Yong-Le
authors.initials: YL
authors.lastname: Zhang
authors.affiliation: Department of Orthopedic, Shanghai Fengxian Central Hospital, Branch of the Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 201400, P.R. China.
authors.forename: Ya-Dong
authors.initials: YD
authors.lastname: Zhang
authors.affiliation: Department of Orthopedic, Shanghai Fengxian Central Hospital, Branch of the Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 201400, P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Xue
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism*; D054377:Chemokine CXCL12 / Q000378:metabolism*; D005260:Female; D006614:Hindlimb / Q000294:innervation / Q000503:physiopathology; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051381:Rats; D019718:Receptors, CXCR4 / Q000378:metabolism*; D015398:Signal Transduction; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000628:therapy*
chemical_list: C575935:CXCL12 protein, rat; D054377:Chemokine CXCL12; C486264:Cxcr4 protein, rat; D019718:Receptors, CXCR4
keywords: bone marrow-derived mesenchymal stem cells; chemotaxis cytokine receptor-4; hind limb motor function; spinal cord injury; stromal cell-derived factor-l