title: Cinnamaldehyde induces apoptosis and reverses epithelial-mesenchymal transition through inhibition of Wnt/β-catenin pathway in non-small cell lung cancer.
abstract: Cinnamaldehyde, the main chemical component of the essential oil separated from the traditional herb Cinnamomum cassia, has been demonstrated to be an efficient cytotoxic agent against several human cancers. The present experiment showed that cinnamaldehyde dose-dependently depresses the proliferation of three types of NSCLC cells and induces cell apoptosis in vitro and in vivo. Moreover, cinnamaldehyde attenuated CoCl2-induced EMT and decreased matrix metalloprotease (MMP) family while the in vivo study showed the same trend. Mechanistically, cinnamaldehyde imitated the suppressive effect of XAV939 on cell motility and EMT which could be impaired by LiCl. Collectively, our research demonstrated for the first time that cinnamaldehyde is able to inhibit NSCLC cell growth by inducing apoptosis and reverse EMT through terminating Wnt/β-catenin pathway, which might supply further insight into cinnamaldehyde-mediated anti-tumor effect against NSCLC for better prognosis.
authors.affiliation: The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of TCM, Nanjing, Jiangsu 210029, China.
authors.forename: Cunen
authors.initials: C
authors.lastname: Wu
authors.affiliation: The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of TCM, Nanjing, Jiangsu 210029, China.
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Zhuang
authors.affiliation: Division of Membrane Dynamics, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan.
authors.forename: Shan
authors.initials: S
authors.lastname: Jiang
authors.affiliation: The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of TCM, Nanjing, Jiangsu 210029, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Tian
authors.affiliation: The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of TCM, Nanjing, Jiangsu 210029, China.
authors.forename: Yuhao
authors.initials: Y
authors.lastname: Teng
authors.affiliation: The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of TCM, Nanjing, Jiangsu 210029, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Chen
authors.affiliation: Liyang Hospital of Traditional Chinese Medicine, Liyang, Jiangsu 213300, China.
authors.forename: Peiqiu
authors.initials: P
authors.lastname: Zheng
authors.affiliation: The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of TCM, Nanjing, Jiangsu 210029, China.
authors.forename: Shenlin
authors.initials: S
authors.lastname: Liu
authors.affiliation: The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of TCM, Nanjing, Jiangsu 210029, China.
authors.forename: Jinyong
authors.initials: J
authors.lastname: Zhou
authors.affiliation: The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of TCM, Nanjing, Jiangsu 210029, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.affiliation: The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of TCM, Nanjing, Jiangsu 210029, China. Electronic address: WRP61@163.com.
authors.forename: Ruiping
authors.initials: R
authors.lastname: Wang
authors.affiliation: The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of TCM, Nanjing, Jiangsu 210029, China. Electronic address: zxvery@126.com.
authors.forename: Xi
authors.initials: X
authors.lastname: Zou
mesh_terms: D000072283:A549 Cells; D000171:Acrolein / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003035:Cobalt / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D060449:Wnt Signaling Pathway / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays; D051176:beta Catenin / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C495270:CTNNB1 protein, human; D051176:beta Catenin; D003035:Cobalt; D000171:Acrolein; C018021:cobaltous chloride; C012843:cinnamaldehyde
keywords: Apoptosis; Cinnamaldehyde; EMT; NSCLC; Wnt/β-catenin pathway