title: Association of single nucleotide polymorphisms in the coding region of Bcl-2 with the occurrence and prognosis of colorectal cancer: A case-control study.
abstract: OBJECTIVE
  To investigate the relationship of single nucleotide polymorphisms in the coding region of Bcl-2 with the occurrence and prognosis of colorectal cancer (CRC).
  
  
  METHODS
  Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was used detect Bcl-2 gene polymorphisms (rs1800477A/G and rs1801018A/G) in 185 patients with CRC (case group) and 177 healthy subjects (control group). The relationships of Bcl-2 gene polymorphisms with clinicopathological features and prognosis of CRC patients were analyzed.
  
  
  RESULTS
  The frequency of GG genotype and G allele of rs1800477A/G in the case group were significantly higher than those in the control group (both P < 0.05). The GG genotype of rs1800477A/G was associated with lymph node metastasis and Dukes' staging of CRC (both P < 0.05). Haplotype analysis demonstrated that the case group had decreased frequency of GA haplotype, but increased frequency of GG haplotype in comparisons to the control group (GA: P = 0.014; GG: P = 0.003). Kaplan-Meier survival analysis showed that the risk of death (within 5 years) in patients carrying GG genotype (rs1800477A/G) was 2.159 as much as that in patients carrying AA + GA genotype. Multivariate analyses showed that Dukes' staging and GG genotype of rs1800477A/G are risk factors for poor prognosis in CRC (Dukes' staging: P = 0.001; GG genotype: P = 0.034).
  
  
  CONCLUSIONS
  Bcl-2 rs1800477A/G polymorphism may be related to the occurrence of CRC, and GG genotype could be a risk factor of poor prognosis in CRC.
authors.affiliation: Department of Laboratory, Yantai Yuhuangding Hospital, Yantai 264000, Shandong, China.
authors.forename: Baohua
authors.initials: B
authors.lastname: Huang
authors.affiliation: Department of Pediatrics, Yantai Yuhuangding Hospital, Yantai 264000, Shandong, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Laboratory, Yantai Yuhuangding Hospital, Yantai 264000, Shandong, China.
authors.forename: Chengming
authors.initials: C
authors.lastname: Sun
authors.affiliation: Department of Laboratory, Yantai Yuhuangding Hospital, Yantai 264000, Shandong, China.
authors.forename: Lipeng
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Laboratory, Yantai Yuhuangding Hospital, Yantai 264000, Shandong, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Yang
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D012307:Risk Factors
chemical_list: D014408:Biomarkers, Tumor; D019253:Proto-Oncogene Proteins c-bcl-2
keywords: Bcl-2; Colorectal cancer; clinicopathological features; gene polymorphism; prognosis