title: hYKL-40 cancer biomarker electroanalysis in serum samples and model cell lysates: capacitive immunosensing compared with enzyme label immunosorbent assays (ELISA).
abstract: Human chitinase 3-like protein 1 (CHI3L1 or hYKL-40), a potential molecular marker for several cancers, was measured in clinical human serum samples and model cell lysates by indirect and competitive enzyme-linked immunosorbent assay (ELISA) and by capacitive immunosensing, so as to evaluate a recently introduced electrochemical method for routine use in cancer-monitoring studies. The clinical samples tested included serum from four healthy individuals, five breast cancer and four glioblastoma patients; cultures of the human monocytic cell line THP-1, known to secrete hYKL-40, and of the human embryonic kidney 293t cell line, which does not express hYKL-40, provided cell lysates and cell culture media for positive and negative bio- and electrochemical control trials. A good agreement was observed between the results of the three tested methods during hYKL-40 quantifications in human serum and cell lysates. Measurements of 'spiked' samples from healthy volunteers, cancer patients and hYKL-40-free 293t cell lysates revealed that capacitive immunosensing and the two types of ELISA all recovered the analyte with an efficiency close to 100%. On this basis, capacitive hYKL-40 immunosensor screening is a promising stand-alone or complementary analytical tool for the analysis of hYKL-40 in serum, and would be useful for the validation of standard ELISA data and also, because of the significantly lower hYKL-40 detection limit of the electroanalytical procedure, would permit assay of the marker and cancer observation at earlier stages than is currently possible using ELISA.
authors.affiliation: School of Chemistry, Suranaree University of Technology, Nakhon Ratchasima, Thailand.
authors.forename: W
authors.initials: W
authors.lastname: Chaocharoen
authors.affiliation: School of Chemistry, Suranaree University of Technology, Nakhon Ratchasima, Thailand and Biochemistry and Electrochemistry Research Unit, Suranaree University of Technology, Nakhon Ratchasima, Thailand. wipa@sut.ac.th schulte@sut.ac.th and Centre of Excellence (CoE) in Advanced Functional Materials, Suranaree University of Technology, Nakhon Ratchasima, Thailand.
authors.forename: A
authors.initials: A
authors.lastname: Schulte
authors.affiliation: School of Chemistry, Suranaree University of Technology, Nakhon Ratchasima, Thailand and Biochemistry and Electrochemistry Research Unit, Suranaree University of Technology, Nakhon Ratchasima, Thailand. wipa@sut.ac.th schulte@sut.ac.th and Centre of Excellence (CoE) in Advanced Functional Materials, Suranaree University of Technology, Nakhon Ratchasima, Thailand.
authors.forename: W
authors.initials: W
authors.lastname: Suginta
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D000071451:Chitinase-3-Like Protein 1 / Q000097:blood*; D055664:Electrochemical Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D057809:HEK293 Cells; D006801:Humans; D057230:Limit of Detection; D000074084:THP-1 Cells
chemical_list: D014408:Biomarkers, Tumor; C084533:CHI3L1 protein, human; D000071451:Chitinase-3-Like Protein 1