title: Activation of dendritic cells by low molecular weight oyster polysaccharides.
abstract: Dendritic cells play a primary role in antigen presentation to CD4+T cells, which initiate acquired immune responses. Therefore, determining positive modulators of dendritic cell activation to improve therapeutic approaches for cancer treatment might be useful. We here investigated the effects of low molecular weight oyster polysaccharides (LMW-OPS) on bone marrow-derived dendritic cells (BMDCs) obtained from mice. LMW-OPS increased the surface expression of major histocompatibility complex class II (MHC-II), CD40 and CD86 in BMDCs and induced the secretion of tumour necrosis factor (TNF)-α and interleukin (IL)-12, which were significantly decreased in the BMDCs derived from MyD88-/-mice but not from the lipopolysaccharide-resistant C3H/HeJ mice. BMDCs treated with LMW-OPS augmented allogeneic CD4+T cell expansion and enhanced secretion of IL-2 and interferon (IFN)-γ but not IL-4. LMW-OPS induced significant increases in ERK and p38 MAPK phosphorylation, but not c-Jun N-terminal kinase (JNK) phosphorylation, in BMDCs. Our results indicate that, in part, LMW-OPS can induce maturation of BMDCs in a MyD88-dependent and Toll-like receptor (TLR) 4-independent manner. LMW-OPS may enhance acquired immunity by modulating the function of dendritic cells.
authors.affiliation: Institute of Tumor Pharmacology, Jining Medical College, Xueyuan Road 669, Rizhao 276826, China. Electronic address: zhongming0410@163.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhong
authors.affiliation: Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, Yamadaoka2-2, Suita, Osaka 565-0871, Japan.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhong
authors.affiliation: People's Hospital of Rizhao, Taian Road 126, Rizhao 276826, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Haike Road 501, Shanghai, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Hu
authors.affiliation: Institute of Tumor Pharmacology, Jining Medical College, Xueyuan Road 669, Rizhao 276826, China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Wang
authors.affiliation: Institute of Tumor Pharmacology, Jining Medical College, Xueyuan Road 669, Rizhao 276826, China.
authors.forename: Rujing
authors.initials: R
authors.lastname: Ren
authors.affiliation: Institute of Tumor Pharmacology, Jining Medical College, Xueyuan Road 669, Rizhao 276826, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Tumor Pharmacology, Jining Medical College, Xueyuan Road 669, Rizhao 276826, China.
authors.forename: Huijei
authors.initials: H
authors.lastname: Gao
authors.affiliation: Institute of Tumor Pharmacology, Jining Medical College, Xueyuan Road 669, Rizhao 276826, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Cui
authors.affiliation: Shandong Analysis and Test Center, Keyuan Road 19, Jinan 250014, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Duan
authors.affiliation: S&V Biological Science and Technology Co., Ltd, Heying Road 8, Nanshao, Changping, Beijing 102200, China.
authors.forename: Jiantu
authors.initials: J
authors.lastname: Che
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000187:drug effects; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D010125:Ostreidae / Q000276:immunology*; D011134:Polysaccharides / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D013601:T-Lymphocytes / Q000276:immunology*; D051197:Toll-Like Receptor 4 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000949:Histocompatibility Antigens Class II; D011134:Polysaccharides; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12
keywords: Dendritic cells; MAPK; Maturation; Oyster; Polysaccharides