title: New Abietane and Kaurane Type Diterpenoids from the Stems of Tripterygium regelii.
abstract: Eleven new abietane type (1‒11), and one new kaurane (12), diterpenes, together with eleven known compounds (13-23), were isolated and identified from the stems ofTripterygium regelii, which has been used as a traditional folk Chinese medicine for the treatment of rheumatoid arthritis in China. The structures of new compounds were characterized by means of the interpretation of high-resolution electrospray ionization mass spectrometry (HRESIMS), extensive nuclear magnetic resonance (NMR) spectroscopic data and comparisons of their experimental CD spectra with calculated electronic circular dichroism (ECD) spectra. Compound1is the first abietane type diterpene with an18→1lactone ring. Compound19was isolated from the plants of theTripterygiumgenus for the first time, and compounds14-17were isolated fromT. regeliifor the first time. Triregelin I (9) showed significant cytotoxicity against A2780 and HepG2 with IC50values of 5.88 and 11.74 µM, respectively. It was found that this compound was inactive against MCF-7 cells. The discovery of these twelve new diterpenes not only provided information on chemical substances ofT. regelii, but also contributed to the chemical diversity of natural terpenoids.
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa, Macau 999078, China. fandongsheng1985@sina.com.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Applied Organic Chemistry and Department of Chemistry, Lanzhou University, Lanzhou 730000, China. zhoushy13@lzu.edu.cn.
authors.forename: Shuangyan
authors.initials: S
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa, Macau 999078, China. zyzheng@must.edu.mo.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa, Macau 999078, China. gyzhu@must.edu.mo.
authors.forename: Guo-Yuan
authors.initials: GY
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa, Macau 999078, China. xjyao@must.edu.mo.|State Key Laboratory of Applied Organic Chemistry and Department of Chemistry, Lanzhou University, Lanzhou 730000, China. xjyao@must.edu.mo.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa, Macau 999078, China. mryang@must.edu.mo.
authors.forename: Ming-Rong
authors.initials: MR
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa, Macau 999078, China. zhjiang@must.edu.mo.
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa, Macau 999078, China. lpbai@must.edu.mo.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Bai
mesh_terms: D045784:Abietanes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002942:Circular Dichroism; D045786:Diterpenes, Kaurane / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D018547:Plant Stems / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D029757:Tripterygium / Q000737:chemistry*
chemical_list: D045784:Abietanes; D045786:Diterpenes, Kaurane
keywords: Tripterygium regelii; cytotoxicity; diterpenoids