title: Platelet, monocyte and neutrophil activation and glucose tolerance in South African Mixed Ancestry individuals.
abstract: Platelet activation has been described in patients with chronic inflammation, however in type 2 diabetes mellitus it remains controversial. We compared levels of platelet leucocyte aggregates, monocyte and granulocyte activation across glucose tolerance statuses in mixed ancestry South Africans. Individuals (206) were recruited from Bellville-South, Cape Town, and included 66% with normal glucose tolerance, 18.7% pre-diabetes, 8.7% screen-detected diabetes and 6.3% known diabetes. Monocyte and neutrophil activation were measured by calculating the percentage of cells expressing CD142 and CD69 while platelet monocyte aggregates were defined as CD14++CD42b+events and platelet neutrophil aggregates as CD16++CD42b+events. The percentage of monocytes and neutrophils expressing CD69 and CD142 was significantly higher in known diabetes and prediabetes, but, lowest in screen-detected diabetes (both p ≤ 0.016). The pattern was similar for platelet monocyte and neutrophil aggregates (both p ≤ 0.003). In robust linear regressions adjusted for age and gender, known diabetes was significantly and positively associated with the percentage of monocytes expressing CD69 [beta 11.06 (p = 0.016)] and CD42b (PMAs) [19.51 (0.003)] as well as the percentage of neutrophils expressing CD69 [14.19 (<0.0001)] and CD42b [17.7 (0.001)]. We conclude that monitoring platelet activation in diagnosed diabetic patients may have a role in the management and risk stratification.
authors.affiliation: Department of Biomedical sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Bellville, South Africa.
authors.forename: Glenda M
authors.initials: GM
authors.lastname: Davison
authors.affiliation: Department of Biomedical sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Bellville, South Africa.
authors.forename: Bongani B
authors.initials: BB
authors.lastname: Nkambule
authors.affiliation: Department of Biomedical sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Bellville, South Africa.
authors.forename: Zibusiso
authors.initials: Z
authors.lastname: Mkandla
authors.affiliation: Department of Biomedical sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Bellville, South Africa.
authors.forename: Gloudina M
authors.initials: GM
authors.lastname: Hon
authors.affiliation: NonCommunicable Diseases Research Unit, South African Medical Research Council, Cape Town, South Africa.|Department of Medicine, University of Cape Town, Cape Town, South Africa.
authors.forename: Andre P
authors.initials: AP
authors.lastname: Kengne
authors.affiliation: Department of Pathology, Faculty of Medicine and Health Sciences, National Health Laboratory Service (NHLS) and Stellenbosch University, Cape Town, South Africa.
authors.forename: Rajiv T
authors.initials: RT
authors.lastname: Erasmus
authors.affiliation: Department of Biomedical sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Bellville, South Africa.
authors.forename: Tandi E
authors.initials: TE
authors.lastname: Matsha
mesh_terms: D000941:Antigens / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism*; D005260:Female; D005789:Genealogy and Heraldry; D005951:Glucose Tolerance Test; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D018375:Neutrophil Activation; D010974:Platelet Aggregation; D012044:Regression Analysis; D013019:South Africa
chemical_list: D000941:Antigens