title: Intestinal Parasite Infections and Accuracy of Direct Thin and Thick Smear, Formol-Ether Sedimentation, Centrifugal Flotation, and Mini-FLOTAC Techniques Among Patients with Gastrointestinal Tract Disorders from the Greater Cairo Region, Egypt.
abstract: The aim of the present study was to assess the frequency of intestinal parasitic infection among patients with gastrointestinal tract disorders from the Greater Cairo region, Egypt. In addition, a comparison was made of the accuracy of direct thin and thick smear, formol-ether sedimentation (FEC), centrifugal flotation (CF), and mini-FLOTAC techniques in the diagnosis of infection. Out of 100 patients, the overall prevalence of parasitic infection was 51%. Only 6% had dual infection.Giardia lambliawas the most common parasite (26%), followed byHymenolepis nana(20%),Entamoeba coli(8%), andEnterobius vermicularis(3%). Except the statistically significant association betweenE. vermicularisinfection and perianal itching and insomnia (P< 0.001), age, gender, and complaints of the examined individuals had no association with prevalence of parasitic infection. Both FEC and CF were equally the most accurate techniques (accuracy = 98.2%, confidence interval [CI] = 0.95-1.0, and κ index = 0.962), whereas the Kato-Katz method was the least accurate (accuracy = 67.5%, CI = 0.57-0.78, and κ index = 0.333). However, mini-FLOTAC-ZnSO4was the most accurate for diagnosis of helminthic infection, and FEC was more accurate for diagnosis of protozoal infection (accuracy = 100%, CI = 1.0-1.0, and κ index = 1).
authors.affiliation: Department of Parasitology, Faculty of Medicine, Benha University, Benha, Egypt.
authors.forename: Atef H
authors.initials: AH
authors.lastname: Hussein
authors.affiliation: Department of Parasitology, Faculty of Medicine, Benha University, Benha, Egypt.
authors.forename: Samia M
authors.initials: SM
authors.lastname: Rashed
authors.affiliation: Department of Parasitology, Faculty of Medicine, Benha University, Benha, Egypt.
authors.forename: Ibrahim A
authors.initials: IA
authors.lastname: El-Hayawan
authors.affiliation: Department of Parasitology, Faculty of Medicine, Benha University, Benha, Egypt.
authors.forename: Nagwa S M
authors.initials: NSM
authors.lastname: Aly
authors.affiliation: Department of Parasitology, Faculty of Medicine, Benha University, Benha, Egypt.
authors.forename: Eman A
authors.initials: EA
authors.lastname: Abou Ouf
authors.affiliation: Department of Parasitology, Faculty of Medicine, Benha University, Benha, Egypt.
authors.forename: Amira T
authors.initials: AT
authors.lastname: Ali
mesh_terms: D000293:Adolescent; D000818:Animals; D002498:Centrifugation; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D004534:Egypt / Q000453:epidemiology; D004747:Entamoeba / Q000302:isolation & purification; D004757:Enterobius / Q000302:isolation & purification; D004987:Ethers; D005243:Feces / Q000469:parasitology; D005260:Female; D005557:Formaldehyde; D041981:Gastrointestinal Tract / Q000469:parasitology; D016829:Giardia lamblia / Q000302:isolation & purification; D006373:Helminthiasis / Q000175:diagnosis* / Q000453:epidemiology*; D006801:Humans; D048189:Hymenolepis nana / Q000302:isolation & purification; D007411:Intestinal Diseases, Parasitic / Q000175:diagnosis* / Q000453:epidemiology*; D008297:Male; D015995:Prevalence; D012680:Sensitivity and Specificity; D013048:Specimen Handling
chemical_list: D004987:Ethers; D005557:Formaldehyde