title: Preclinical models in HNSCC: A comprehensive review.
abstract: Head and neck cancer remains a significant public health concern. About 60% of patients die within 5years due to local recurrence. Head and neck squamous cell carcinoma (HNSCC) cell lines are important preclinical models in the search for new therapies against this disease. Furthermore, there is a need to test novel drugs before introduction into clinical practice. A preclinical model that closely resembles the in vivo situation would be highly valuable. In the last few decades, a multicellular spheroid model has gained attention as its behavior was comparable to in vivo tumors. Basic research is necessary to achieve an understanding of the normal and pathological state but cannot, in itself, provide sufficient information for clinical applications. Indeed, animal models are an inevitable prelude to assess the efficacy of new therapeutic approaches in HNSCC. The present review proposes an overview of HNSCC pre-clinical models in order to further understand the oncogenic properties for HNSCC and translate these findings into clinic for patients.
authors.affiliation: Université Lyon 1, Faculté de Médecine-Lyon-Sud, 69921 Oullins, France; Laboratoire de Radiobiologie Cellulaire et Moléculaire, CNRS UMR 5822, Institut de Physique Nucléaire de Lyon, IPNL, 69622 Villeurbanne, France; Département de Radiothérapie, Institut de Cancérologie de la Loire - Lucien Neuwirth, 42270 St Priest en Jarez, France.
authors.forename: Benoite
authors.initials: B
authors.lastname: Méry
authors.affiliation: Département de Radiothérapie, Institut de Cancérologie de la Loire - Lucien Neuwirth, 42270 St Priest en Jarez, France.
authors.forename: Chloé
authors.initials: C
authors.lastname: Rancoule
authors.affiliation: Université Lyon 1, Faculté de Médecine-Lyon-Sud, 69921 Oullins, France; Laboratoire de Radiobiologie Cellulaire et Moléculaire, CNRS UMR 5822, Institut de Physique Nucléaire de Lyon, IPNL, 69622 Villeurbanne, France; Département de Radiothérapie, Institut de Cancérologie de la Loire - Lucien Neuwirth, 42270 St Priest en Jarez, France.
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Guy
authors.affiliation: Université Lyon 1, Faculté de Médecine-Lyon-Sud, 69921 Oullins, France; Laboratoire de Radiobiologie Cellulaire et Moléculaire, CNRS UMR 5822, Institut de Physique Nucléaire de Lyon, IPNL, 69622 Villeurbanne, France; Département de Radiothérapie, Institut de Cancérologie de la Loire - Lucien Neuwirth, 42270 St Priest en Jarez, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Espenel
authors.affiliation: Université Lyon 1, Faculté de Médecine-Lyon-Sud, 69921 Oullins, France; Laboratoire de Radiobiologie Cellulaire et Moléculaire, CNRS UMR 5822, Institut de Physique Nucléaire de Lyon, IPNL, 69622 Villeurbanne, France; Hospices Civils de Lyon, 69229 Lyon, France.
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Wozny
authors.affiliation: Université Lyon 1, Faculté de Médecine-Lyon-Sud, 69921 Oullins, France; Laboratoire de Radiobiologie Cellulaire et Moléculaire, CNRS UMR 5822, Institut de Physique Nucléaire de Lyon, IPNL, 69622 Villeurbanne, France.
authors.forename: Priscilla
authors.initials: P
authors.lastname: Battiston-Montagne
authors.affiliation: Université Lyon 1, Faculté de Médecine-Lyon-Sud, 69921 Oullins, France; Laboratoire de Radiobiologie Cellulaire et Moléculaire, CNRS UMR 5822, Institut de Physique Nucléaire de Lyon, IPNL, 69622 Villeurbanne, France; Hospices Civils de Lyon, 69229 Lyon, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Ardail
authors.affiliation: Institut de Physique Nucléaire de Lyon, IPNL, 69622 Villeurbanne, France.
authors.forename: Michael
authors.initials: M
authors.lastname: Beuve
authors.affiliation: Laboratoire de Radiobiologie Cellulaire et Moléculaire, CNRS UMR 5822, Institut de Physique Nucléaire de Lyon, IPNL, 69622 Villeurbanne, France; Hospices Civils de Lyon, 69229 Lyon, France.
authors.forename: Gersende
authors.initials: G
authors.lastname: Alphonse
authors.affiliation: Université Lyon 1, Faculté de Médecine-Lyon-Sud, 69921 Oullins, France; Laboratoire de Radiobiologie Cellulaire et Moléculaire, CNRS UMR 5822, Institut de Physique Nucléaire de Lyon, IPNL, 69622 Villeurbanne, France; Hospices Civils de Lyon, 69229 Lyon, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Rodriguez-Lafrasse
authors.affiliation: Université Lyon 1, Faculté de Médecine-Lyon-Sud, 69921 Oullins, France; Laboratoire de Radiobiologie Cellulaire et Moléculaire, CNRS UMR 5822, Institut de Physique Nucléaire de Lyon, IPNL, 69622 Villeurbanne, France; Département de Radiothérapie, Institut de Cancérologie de la Loire - Lucien Neuwirth, 42270 St Priest en Jarez, France. Electronic address: nicolas.magne@icloire.fr.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Magné
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D004195:Disease Models, Animal; D006258:Head and Neck Neoplasms / Q000473:pathology*; D006801:Humans; D051379:Mice; D000077195:Squamous Cell Carcinoma of Head and Neck
keywords: HNSCC preclinical models; HNSCC translational research; In vitro and in vivo assays; Specific cell lines; Three-dimensional spheroid model; Transgenic and syngeneic animal models