title: [Function and mechanism of zinc finger protein ZNF50 in inhibiting the growth of esophageal squamous cell carcinoma cells].
abstract: Objective:To investigate the function and mechanism of zinc finger protein 750 (ZNF750) in esophageal squamous cell carcinoma (ESCC).Methods:Xenograft in nude mice was applied to detect the tumorigenesis of ZNF750-depleted ESCC cells. Western blot was performed to observe the expression of downstream target protein of ZNF750 in ESCC cell lines and xenograft tumor tissues in which ZNF750 was knocked down. 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H tetrazolium bromide (MTT) assay was used to determine the proliferation of ZNF750 stably depleted cells after restoration of its target protein.Results:The tumor weight of blank control, negative control and ZNF750 knockdown groups was 137±26 mg, 161±31 mg and 463±89 mg, respectively, with a statistically significant difference (P<0.01). The expressions of Kruppel-like factor 4 (KLF4) in ZNF750-depleted ESCC cells and its derived tumor tissue xenograft in nude mice were significantly down-regulated. Restoration of KLF4 in ZNF750 stably depleted cells significantly inhibited the cell proliferation (P<0.01).Conclusions:ZNF750 may be a new tumor suppressor in the tumorigenesis of ESCC, and the inhibition of cell proliferation induced by ZNF750 may be partially through the regulation of KLF4 expression.
authors.affiliation: Translational Medicine Research Center, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.affiliation: Translational Medicine Research Center, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Bi
authors.affiliation: Translational Medicine Research Center, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Material Evidence, College of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: F
authors.initials: F
authors.lastname: Liu
authors.affiliation: Translational Medicine Research Center, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Y K
authors.initials: YK
authors.lastname: Li
authors.affiliation: Translational Medicine Research Center, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Cui
authors.affiliation: Translational Medicine Research Center, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: F
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of General Surgery, The First Affiliated Hospital, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Thoracic Surgery, Shanxi Cancer Hospital, Taiyuan 030013, China.
authors.forename: B
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Pathology, College of Preclinical Medicine, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D004938:Esophageal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D000077277:Esophageal Squamous Cell Carcinoma; D055785:Gene Knockdown Techniques; D016147:Genes, Tumor Suppressor / Q000502:physiology; D006801:Humans; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D009363:Neoplasm Proteins / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D025521:Tumor Suppressor Proteins; D016335:Zinc Fingers / Q000235:genetics / Q000502:physiology*
chemical_list: C000715467:KLF4 protein, human; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; D009363:Neoplasm Proteins; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; C570799:ZNF750 protein, human
keywords: Carcinoma, squamous cell; Esophageal neoplasms; Genome-wide association study; Kruppel-like factor 4; Zinc finger protein 750