title: Targeted integration of genes in Xenopus tropicalis.
abstract: With the successful establishment of both targeted gene disruption and integration methods in the true diploid frog Xenopus tropicalis, this excellent vertebrate genetic model now is making a unique contribution to modelling human diseases. Here, we summarize our efforts on establishing homologous recombination-mediated targeted integration in Xenopus tropicalis, the usefulness, and limitation of targeted integration via the homology-independent strategy, and future directions on how to further improve targeted gene integration in Xenopus tropicalis.
authors.affiliation: Shenzhen Key Laboratory of Cell Microenvironment, Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, China.
authors.forename: Zhaoying
authors.initials: Z
authors.lastname: Shi
authors.affiliation: Shenzhen Key Laboratory of Cell Microenvironment, Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Tian
authors.affiliation: Shenzhen Key Laboratory of Cell Microenvironment, Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, China.
authors.forename: Huhu
authors.initials: H
authors.lastname: Xin
authors.affiliation: Shenzhen Key Laboratory of Cell Microenvironment, Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, China.
authors.forename: Jingru
authors.initials: J
authors.lastname: Lian
authors.affiliation: DanStem, University of Copenhagen, 3B Blegdamsvej, DK-2200, Copenhagen N, Denmark.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Guo
authors.affiliation: Shenzhen Key Laboratory of Cell Microenvironment, Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, China.
authors.forename: Yonglong
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D018507:Gene Expression Regulation, Developmental; D018390:Gene Targeting; D006801:Humans; D014981:Xenopus / Q000235:genetics* / Q000254:growth & development; D029867:Xenopus Proteins / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D029867:Xenopus Proteins
keywords: heritable; homology-dependent; homology-independent; knock-in