title: Stabilization of freeze-dried Lactobacillus paracasei subsp. paracasei JCM 8130Twith the addition of disaccharides, polymers, and their mixtures.
abstract: Although freeze-drying is a widely used dehydration technique for the stabilizing of unstable lactic acid bacteria, Lactobacillus paracasei subsp. paracasei JCM 8130T(L. paracasei) is destabilized after freeze-drying and subsequent storage. In order to improve the stability of freeze-dried L. paracasei, effects of disaccharides (sucrose and trehalose), polymers (maltodextrin; MD and bovine serum albumin; BSA), and their mixtures on the survival rate of freeze-dried L. paracasei were investigated. The survival rate of non-additive sample decreased slightly after freeze-drying but decreased drastically after subsequent storage at 37 °C for 4 weeks. The reduction was diminished by the addition of disaccharides and polymers. The stabilizing effect of disaccharides was not affected by the co-addition of MD. In contrast, the disaccharide-BSA mixtures had a synergistic stabilizing effect, and the survival rates were largely maintained even after storage. It is suggested that the synergistic effect originates from the conformational stabilization of the dehydrated bacteria.
authors.affiliation: a Department of Biofunctional Science and Technology, Graduate School of Biosphere Science , Hiroshima University , Hiroshima , Japan.
authors.forename: Da
authors.initials: D
authors.lastname: Teng
authors.affiliation: a Department of Biofunctional Science and Technology, Graduate School of Biosphere Science , Hiroshima University , Hiroshima , Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Kawai
authors.affiliation: a Department of Biofunctional Science and Technology, Graduate School of Biosphere Science , Hiroshima University , Hiroshima , Japan.
authors.forename: Shuto
authors.initials: S
authors.lastname: Mikajiri
authors.affiliation: a Department of Biofunctional Science and Technology, Graduate School of Biosphere Science , Hiroshima University , Hiroshima , Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Hagura
mesh_terms: D000818:Animals; D002417:Cattle; D004187:Disaccharides / Q000737:chemistry; D005516:Food Microbiology; D005612:Freeze Drying; D006801:Humans; D000069980:Lacticaseibacillus paracasei / Q000737:chemistry / Q000187:drug effects*; D011134:Polysaccharides / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology; D013696:Temperature; D014199:Trehalose / Q000494:pharmacology; D014867:Water / Q000737:chemistry
chemical_list: D004187:Disaccharides; D011134:Polysaccharides; D014867:Water; D012710:Serum Albumin, Bovine; D013395:Sucrose; C008315:maltodextrin; D014199:Trehalose
keywords: freeze-dry; glass transition temperature; lactic acid bacteria; survival rate; water activity