title: Microbial BOD sensors based on Zr (IV)-loaded collagen fiber.
abstract: Biochemical oxygen demand (BOD) sensors based on Zr (IV)-loaded collagen fiber (ZrCF), a novel material with great porous structure, were developed. This novel material shows adsorbability by microorganisms. Saccharomyces cerevisiae and Escherichia coli were used for the construction of BOD sensors. Factors affecting BOD sensor performance were examined. The ZrCF-based BOD sensor showed different sensitivities and linear response ranges with different biofilm densities. The amount of microorganisms strongly affected the performance of the BOD sensor. Poor permeability of previously reported immobilization carriers were greatly circumvented by ZrCF. The service life of the ZrCF-based BOD sensor was more than 42 days. The immobilized microorganisms can be stored for more than 6 months under 4°C in PB solution. There was good correlation between the results of the sensor method and the standard 5-day BOD method in the determination of pure organic substrates and real water samples.
authors.affiliation: College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.affiliation: College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China. Electronic address: helifood@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: He
authors.affiliation: College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
authors.forename: Shujuan
authors.initials: S
authors.lastname: Chen
authors.affiliation: The Lab of Microbiology, Sichuan Agricultural University, Dujiangyan, Sichuan 611830, PR China.
authors.forename: Likou
authors.initials: L
authors.lastname: Zou
authors.affiliation: College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
authors.forename: Kang
authors.initials: K
authors.lastname: Zhou
authors.affiliation: College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Ao
authors.affiliation: College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
authors.forename: Shuliang
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
authors.forename: Xinjie
authors.initials: X
authors.lastname: Hu
authors.affiliation: College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
authors.forename: Guoquan
authors.initials: G
authors.lastname: Han
mesh_terms: D018441:Biofilms; D057919:Biological Oxygen Demand Analysis / Q000379:methods*; D015374:Biosensing Techniques / Q000379:methods*; D018914:Cells, Immobilized / Q000378:metabolism; D003094:Collagen; D004926:Escherichia coli / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D054712:Salinity; D013696:Temperature; D062065:Wastewater / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis; D015040:Zirconium
chemical_list: D003094:Collagen; D062065:Wastewater; D014874:Water Pollutants, Chemical; D015040:Zirconium
keywords: BOD; Immobilization carrier; Microbial biosensor; ZrCF