title: RSM22, mtYsxC and PNKD-like proteins are required for mitochondrial translation in Trypanosoma brucei.
abstract: Mitochondrial ribosomes evolved from prokaryotic ribosomes, with which they therefore share more common features than with their counterparts in the cytosol. Yet, mitochondrial ribosomes are highly diverse in structure and composition, having undergone considerable changes, including reduction of their RNA component and varying degree of acquisition of novel proteins in various phylogenetic lineages. Here, we present functional analysis of three putative mitochondrial ribosome-associated proteins (RSM22, mtYsxC and PNKD-like) in Trypanosoma brucei, originally identified by database mining. While in other systems the homologs of RSM22 are known as components of mitochondrial ribosomes, YsxC was linked with ribosomes only in bacteria. The PNKD-like protein shows similarity to a human protein, the defects of which cause PNKD (paroxysmal non-kinesigenic dyskinesia). Here we show that all three proteins are important for the growth of T. brucei. They play an important function in mitochondrial translation, as their ablation by RNAi rapidly and severely affected the de novo synthesis of mitochondrial proteins. Moreover, following the RNAi-mediated depletion of RSM22, structure of the small subunit of mitochondrial ribosome becomes severely compromised, suggesting a role of RSM22 in ribosomal assembly and/or stability.
authors.affiliation: Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic; Faculty of Science, University of South Bohemia, České Budějovice (Budweis), Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Týč
authors.affiliation: Department of Biology, University of California, Riverside, CA, USA.
authors.forename: Lucie
authors.initials: L
authors.lastname: Novotná
authors.affiliation: Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic.
authors.forename: Priscilla
authors.initials: P
authors.lastname: Peña-Diaz
authors.affiliation: Department of Biology, University of California, Riverside, CA, USA.
authors.forename: Dmitri A
authors.initials: DA
authors.lastname: Maslov
authors.affiliation: Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic; Faculty of Science, University of South Bohemia, České Budějovice (Budweis), Czech Republic; Canadian Institute for Advanced Research, Toronto, Canada. Electronic address: jula@paru.cas.cz.
authors.forename: Julius
authors.initials: J
authors.lastname: Lukeš
mesh_terms: D019295:Computational Biology; D020868:Gene Silencing; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D017386:Sequence Homology, Amino Acid; D014346:Trypanosoma brucei brucei / Q000235:genetics / Q000254:growth & development*
chemical_list: D024101:Mitochondrial Proteins; D015800:Protozoan Proteins
keywords: LSU; Mitochondrial ribosome; PNKD; SSU; YihA; YsxC