title: The Power of CRISPR-Cas9-Induced Genome Editing to Speed Up Plant Breeding.
abstract: Genome editing with engineered nucleases enabling site-directed sequence modifications bears a great potential for advanced plant breeding and crop protection. Remarkably, the RNA-guided endonuclease technology (RGEN) based on the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) is an extremely powerful and easy tool that revolutionizes both basic research and plant breeding. Here, we review the major technical advances and recent applications of the CRISPR-Cas9 system for manipulation of model and crop plant genomes. We also discuss the future prospects of this technology in molecular plant breeding.
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, Gatersleben, 06466 Stadt Seeland, Germany.
authors.forename: Hieu X
authors.identifier: 0000-0003-1230-4127
authors.initials: HX
authors.lastname: Cao
authors.affiliation: School of Agriculture and Biology, Shanghai Jiaotong University, 800 Dong Chuan Road, Shanghai 200240, China.
authors.forename: Wenqin
authors.initials: W
authors.lastname: Wang
authors.affiliation: Institute of Genome Research, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay, Hanoi, Vietnam.
authors.forename: Hien T T
authors.initials: HT
authors.lastname: Le
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, Gatersleben, 06466 Stadt Seeland, Germany.
authors.forename: Giang T H
authors.identifier: 0000-0001-8394-9067
authors.initials: GT
authors.lastname: Vu