title: Structural Characterization of Monomeric/Dimeric State of p59fynSH2 Domain.
abstract: Src homology 2 (SH2) domains are key modulators in various signaling pathways allowing the recognition of phosphotyrosine sites of different proteins. Despite the fact that SH2 domains acquire their biological functions in a monomeric state, a multitude of reports have shown their tendency to dimerize. Here, we provide a technical description on how to isolate and characterize by gel filtration, circular dichroism (CD), and nuclear magnetic resonance (NMR) each conformational state of p59fynSH2 domain.
authors.affiliation: Jean Jeener NMR Center, Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussel, Belgium.|Structural Biology Research Center, VIB, Pleinlaan 2, 1050, Brussel, Belgium.
authors.forename: Radu
authors.initials: R
authors.lastname: Huculeci
authors.affiliation: Jean Jeener NMR Center, Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussel, Belgium.|Structural Biology Research Center, VIB, Pleinlaan 2, 1050, Brussel, Belgium.
authors.forename: Fabien
authors.initials: F
authors.lastname: Kieken
authors.affiliation: Jean Jeener NMR Center, Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussel, Belgium.|Structural Biology Research Center, VIB, Pleinlaan 2, 1050, Brussel, Belgium.
authors.forename: Abel
authors.initials: A
authors.lastname: Garcia-Pino
authors.affiliation: Jean Jeener NMR Center, Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussel, Belgium.|Structural Biology Research Center, VIB, Pleinlaan 2, 1050, Brussel, Belgium.
authors.forename: Lieven
authors.initials: L
authors.lastname: Buts
authors.affiliation: Jean Jeener NMR Center, Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussel, Belgium.|Structural Biology Research Center, VIB, Pleinlaan 2, 1050, Brussel, Belgium.
authors.forename: Nico
authors.initials: N
authors.lastname: van Nuland
authors.affiliation: MLG, Département d'Informatique, Université Libre de Bruxelles, Boulevard du Triomphe CP212, 1050, Brussels, Belgium. tlenaert@ulb.ac.be.|AI-lab, Vakgroep Computerwetenschappen, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussel, Belgium. tlenaert@ulb.ac.be.|Interuniversity Institute of Bioinformatics Brussels (IB2), ULB-VUB, Boulevard du Triomphe CP 263, 1050, Brussels, Belgium. tlenaert@ulb.ac.be.
authors.forename: Tom
authors.initials: T
authors.lastname: Lenaerts
mesh_terms: D002850:Chromatography, Gel; D002942:Circular Dichroism; D003001:Cloning, Molecular; D015870:Gene Expression; D019906:Nuclear Magnetic Resonance, Biomolecular; D010957:Plasmids / Q000235:genetics; D011485:Protein Binding; D055503:Protein Multimerization; D051076:Proto-Oncogene Proteins c-fyn / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D011993:Recombinant Fusion Proteins; D013329:Structure-Activity Relationship; D018909:src Homology Domains
chemical_list: D011993:Recombinant Fusion Proteins; D051076:Proto-Oncogene Proteins c-fyn
keywords: Circular dichroism (CD); Gel filtration chromatography; Monomer/dimer; Nuclear magnetic resonance (NMR); SH2 domain