title: Association of Toll-Like Receptor 3 and Toll-Like Receptor 9 Single Nucleotide Polymorphisms with Hepatitis C Virus Infection and Hepatic Fibrosis in Egyptian Patients.
abstract: Toll-like receptors (TLRs) are recognized as fundamental contributors to the immune system function against infections. Hepatitis C virus (HCV) infection represents a global health problem especially in Egypt having the highest HCV prevalence worldwide where HCV infection is a continuing epidemic. The aim of the present study was to investigate the possible association between genetic variation in TLR-3 and TLR-9 and HCV infection and hepatic fibrosis in chronic HCV-positive Egyptian patients. The present study included 100 naïve chronic HCV-positive patients and 100 age- and sex-matched healthy controls. Genotyping of TLR-3 (_7 C/A [rs3775296]), TLR-3 (c.1377C/T [rs3775290]) and TLR-9 (1237T/C [rs5743836]) were done by polymerase chain reaction restriction fragment length polymorphism technique. Frequency of polymorphic genotypes in TLR-3 (_7 C/A), TLR-3 (c.1377C/T) and TLR-9 (1237T/C) were not significantly different between studied HCV-positive patients and controls withPvalues 0.121, 0.112, and 0.683, respectively. TLR-3 c.1377 T-allele was associated with advanced stage of hepatic fibrosis (P= 0.003).
authors.affiliation: Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors.forename: Rania A
authors.initials: RA
authors.lastname: Zayed
authors.affiliation: Department of Endemic Medicine and Hepato-gastroentrology, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors.forename: Dalia
authors.initials: D
authors.lastname: Omran
authors.affiliation: Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors.forename: Doha A
authors.initials: DA
authors.lastname: Mokhtar
authors.affiliation: Department of Endemic Medicine and Hepato-gastroentrology, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors.forename: Zinab
authors.initials: Z
authors.lastname: Zakaria
authors.affiliation: Community Medicine Department, National Liver Institute, Menofia University, Menofia, Egypt.
authors.forename: Sameera
authors.initials: S
authors.lastname: Ezzat
authors.affiliation: Specialized Liver Unit, Kasr Alainy Hospital, Cairo University, Cairo, Egypt.
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Soliman
authors.affiliation: National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt.
authors.forename: Lamiaa
authors.initials: L
authors.lastname: Mobarak
authors.affiliation: Tropical Medicine Department, Cairo Fatemic Hospital, Ministry of Health, Cairo, Egypt.
authors.forename: Hossam
authors.initials: H
authors.lastname: El-Sweesy
authors.affiliation: Clinical Pathology Department, National Institute of Neuromotor System, Cairo, Egypt.
authors.forename: Ghada
authors.initials: G
authors.lastname: Emam
mesh_terms: D000328:Adult; D000483:Alleles; D015992:Body Mass Index; D016022:Case-Control Studies; D004534:Egypt; D005260:Female; D020022:Genetic Predisposition to Disease; D060005:Genotyping Techniques; D016174:Hepacivirus / Q000302:isolation & purification; D006526:Hepatitis C / Q000453:epidemiology* / Q000235:genetics; D006801:Humans; D008103:Liver Cirrhosis / Q000453:epidemiology* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D015995:Prevalence; D051196:Toll-Like Receptor 3 / Q000235:genetics*; D051217:Toll-Like Receptor 9 / Q000235:genetics*
chemical_list: C495344:TLR3 protein, human; C495371:TLR9 protein, human; D051196:Toll-Like Receptor 3; D051217:Toll-Like Receptor 9