title: Oilseed rape NAC56 transcription factor modulates reactive oxygen species accumulation and hypersensitive response-like cell death.
abstract: The NAC (NAM, ATAF1/2, CUC2) transcription factor gene family is plant-specific and plays diverse roles in development and responses to abiotic stresses and pathogen challenge. Oilseed rape (Brassica napus) or canola is an important oil crop worldwide, however, the function of NAC genes in it remains largely elusive. In the present study, we identified and characterized the NAC56 gene isolated from oilseed rape. Expression of BnaNAC56 was induced by abscisic acid (ABA), jasmonic acid (JA), methyl viologen (MV) and a necrotrophic fungal pathogen Sclerotinia sclerotiorum, but repressed by cold. BnaNAC56 is a transcription activator and localized to nuclei. Overexpression of BnaNAC56 induced reactive oxygen species (ROS) accumulation and hypersensitive response (HR)-like cell death, with various physiological measurements supporting these. Furthermore, BnaNAC56 expression caused evident nuclear DNA fragmentation. Moreover, quantitative reverse transcription PCR (qRT-PCR) analysis identified that the expression levels of multiple genes regulating ROS homeostasis, cell death and defense response were significantly induced. Using a dual luciferase reporter assay, we further confirmed that BnaNAC56 could activate the expression of a few ROS- and cell death-related genes. In summary, our data demonstrate that BnaNAC56 functions as a stress-responsive transcriptional activator and plays a role in modulating ROS accumulation and cell death.
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A & F University, Shaanxi, China.
authors.forename: Qinqin
authors.initials: Q
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A & F University, Shaanxi, China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Niu
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A & F University, Shaanxi, China.
authors.forename: Jingli
authors.initials: J
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A & F University, Shaanxi, China.
authors.forename: Bisi
authors.initials: B
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A & F University, Shaanxi, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A & F University, Shaanxi, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A & F University, Shaanxi, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A & F University, Shaanxi, China.
authors.forename: Yuan-Qing
authors.identifier: http://orcid.org/0000-0003-1367-922X
authors.initials: YQ
authors.lastname: Jiang
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D001203:Ascomycota / Q000502:physiology; D029688:Brassica napus / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D016923:Cell Death / Q000235:genetics; D003517:Cyclopentanes / Q000378:metabolism; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D054883:Oxylipins / Q000378:metabolism; D010269:Paraquat / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D014157:Transcription Factors; C011006:jasmonic acid; D000040:Abscisic Acid; D010269:Paraquat