title: Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome.
abstract: Malignant Catarrhal Fever (MCF) was investigated in the central nervous system of cattle with neurological syndrome. Two-hundred-ninety samples were analyzed by histology, and molecular methods to detect ovine herpesvirus type 2 (OvHV-2) were optimized and validated. The qualitative polymerase chain reaction (qualitative PCR) analytical sensitivity was 101DNA copies/μL and found 4.8% (14/290) positive for OvHV-2. The quantitative polymerase chain reaction (qPCR) analytical sensitivity was 100DNA copy/μL and 5.9% (17/290) positivity, with 47.1% (8/17) of the positive samples presenting histological evidence of non-purulent meningo-encephalitis. The qualitative PCR products (422bp of the ORF75 region) were sequenced and submitted to phylogenetic analysis. Identity matrices showed 100% similarity in OvHV-2 samples obtained in this study and those recovered from GenBank, corroborating other studies.
authors.affiliation: Instituto Biológico, Laboratório de Viroses de Bovídeos, São Paulo, SP, Brazil. Electronic address: mairamartins.bio@gmail.com.
authors.forename: Maira de S N
authors.initials: MSN
authors.lastname: Martins
authors.affiliation: Complexo Educacional Faculdade Metropolitanas Unidas, São Paulo, SP, Brazil.
authors.forename: Alessandra M M G de
authors.initials: AMMG
authors.lastname: Castro
authors.affiliation: Instituto Biológico, Laboratório de Viroses de Bovídeos, São Paulo, SP, Brazil.
authors.forename: Michele Dos S
authors.initials: MDS
authors.lastname: Lima
authors.affiliation: Instituto Biológico, Laboratório de Viroses de Bovídeos, São Paulo, SP, Brazil.
authors.forename: Vivian da S C
authors.initials: VDSC
authors.lastname: Pinto
authors.affiliation: Instituto Biológico, Laboratório de Viroses de Bovídeos, São Paulo, SP, Brazil.
authors.forename: Thaís G da
authors.initials: TGD
authors.lastname: Silva
authors.affiliation: Instituto Biológico, Laboratório de Viroses de Bovídeos, São Paulo, SP, Brazil.
authors.forename: Claudia Del
authors.initials: CD
authors.lastname: Fava
authors.affiliation: Coordenadoria de Defesa Agropecuária do Estado de São Paulo, São Paulo, SP, Brazil.
authors.forename: Claudio Regis
authors.initials: CR
authors.lastname: Depes
authors.affiliation: Instituto Biológico, Laboratório de Viroses de Bovídeos, São Paulo, SP, Brazil.
authors.forename: Liria H
authors.initials: LH
authors.lastname: Okuda
authors.affiliation: Instituto Biológico, Laboratório de Viroses de Bovídeos, São Paulo, SP, Brazil.
authors.forename: Edviges M
authors.initials: EM
authors.lastname: Pituco
mesh_terms: D000818:Animals; D001938:Brazil; D002417:Cattle; D016000:Cluster Analysis; D005838:Genotype; D006564:Herpesviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006651:Histocytochemistry; D008304:Malignant Catarrh / Q000175:diagnosis* / Q000473:pathology*; D008853:Microscopy; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
keywords: Histopathology; Ovine herpesvirus type 2 (OvHV-2); Phylogenetic analysis; Qualitative PCR; Quantitative PCR