title: A Clostridium difficile-Specific, Gel-Forming Protein Required for Optimal Spore Germination.
abstract: Clostridium difficile is a Gram-positive spore-forming obligate anaerobe that is a leading cause of antibiotic-associated diarrhea worldwide. In order for C. difficile to initiate infection, its aerotolerant spore form must germinate in the gut of mammalian hosts. While almost all spore-forming organisms use transmembrane germinant receptors to trigger germination, C. difficile uses the pseudoprotease CspC to sense bile salt germinants. CspC activates the related subtilisin-like protease CspB, which then proteolytically activates the cortex hydrolase SleC. Activated SleC degrades the protective spore cortex layer, a step that is essential for germination to proceed. Since CspC incorporation into spores also depends on CspA, a related pseudoprotease domain, Csp family proteins play a critical role in germination. However, how Csps are incorporated into spores remains unknown. In this study, we demonstrate that incorporation of the CspC, CspB, and CspA germination regulators into spores depends on CD0311 (renamed GerG), a previously uncharacterized hypothetical protein. The reduced levels of Csps in gerG spores correlate with reduced responsiveness to bile salt germinants and increased germination heterogeneity in single-spore germination assays. Interestingly, asparagine-rich repeat sequences in GerG's central region facilitate spontaneous gel formation in vitro even though they are dispensable for GerG-mediated control of germination. Since GerG is found exclusively in C. difficile, our results suggest that exploiting GerG function could represent a promising avenue for developing C. difficile-specific anti-infective therapies.
  
  
  
  The spore-forming bacterium Clostridium difficile is a leading cause of health care-associated infections. While a subset of antibiotics can treat C. difficile infections (CDIs), the primary determinant of CDI disease susceptibility is prior antibiotic exposure, since it reduces the colonization resistance conferred by a diverse microflora. Thus, therapies that minimize perturbations to the gut microbiome should be more effective at reducing CDIs and their recurrence, the main source of disease complications. Given that spore germination is essential for C. difficile to initiate infection and that C. difficile uses a unique pathway to initiate germination, methods that inhibit distinct elements of germination could selectively prevent C. difficile disease recurrence. Here, we identify GerG as a C. difficile-specific protein that controls the incorporation of germinant signaling proteins into spores. Since gerG mutant spores exhibit germination defects and are less responsive to germinant, GerG may represent a promising target for developing therapeutics against CDI.
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, Vermont, USA.
authors.forename: M Lauren
authors.initials: ML
authors.lastname: Donnelly
authors.affiliation: Department of Physics, East Carolina University, Greenville, North Carolina, USA.
authors.forename: William
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Physics, East Carolina University, Greenville, North Carolina, USA.
authors.forename: Yong-Qing
authors.initials: YQ
authors.lastname: Li
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, Vermont, USA.|Program in Cellular, Molecular & Biomedical Sciences, University of Vermont, Burlington, Vermont, USA.
authors.forename: Lauren
authors.initials: L
authors.lastname: Hinkel
authors.affiliation: Department of Molecular Biology and Biophysics, University of Connecticut Health Center, Farmington, Connecticut, USA.
authors.forename: Peter
authors.initials: P
authors.lastname: Setlow
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, Vermont, USA aimee.shen@tufts.edu.|Department of Molecular Biology and Microbiology, Tufts University Medical School, Boston, Massachusetts, USA.
authors.forename: Aimee
authors.initials: A
authors.lastname: Shen
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D016360:Clostridioides difficile / Q000254:growth & development*; D010447:Peptide Hydrolases / Q000378:metabolism; D013171:Spores, Bacterial / Q000254:growth & development*
chemical_list: D001426:Bacterial Proteins; D010447:Peptide Hydrolases