title: Inhibition of voltage-dependent potassium channels mediates cAMP-potentiated insulin secretion in rat pancreatic β cells.
abstract: Insulin secretion is essential for maintenance of glucose homeostasis. An important intracellular signal regulating insulin secretion is cAMP. In this report, we showed that an increase of cAMP induced by adenylyl cyclase (AC) activator forskolin or by cAMP analog db-cAMP not only potentiated insulin secretion but also inhibited Kv channels, and these effects were reversed by AC inhibitor SQ22536. The cAMP-mediated Kv channel inhibition resulted in prolongation of action potential duration, which partly accounts for the elevation of intracellular Ca2+induced by activation of cAMP signaling. Taken together, the results suggest that Kv channels are involved in cAMP-potentiated insulin secretion in pancreatic β cells.
authors.affiliation: a Department of Endocrinology , the First Hospital of Shanxi Medical University, Shanxi Medical University , Taiyuan , China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Liu
authors.affiliation: b Department of Pharmacology , Shanxi Medical University , Taiyuan , China.
authors.forename: Xiangqin
authors.initials: X
authors.lastname: Zhong
authors.affiliation: b Department of Pharmacology , Shanxi Medical University , Taiyuan , China.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Ding
authors.affiliation: b Department of Pharmacology , Shanxi Medical University , Taiyuan , China.
authors.forename: Lele
authors.initials: L
authors.lastname: Ren
authors.affiliation: a Department of Endocrinology , the First Hospital of Shanxi Medical University, Shanxi Medical University , Taiyuan , China.|b Department of Pharmacology , Shanxi Medical University , Taiyuan , China.|c Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University , Taiyuan , China.
authors.forename: Tao
authors.initials: T
authors.lastname: Bai
authors.affiliation: b Department of Pharmacology , Shanxi Medical University , Taiyuan , China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Liu
authors.affiliation: b Department of Pharmacology , Shanxi Medical University , Taiyuan , China.|c Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University , Taiyuan , China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Liu
authors.affiliation: b Department of Pharmacology , Shanxi Medical University , Taiyuan , China.
authors.forename: Yangyan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: b Department of Pharmacology , Shanxi Medical University , Taiyuan , China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Guo
authors.affiliation: b Department of Pharmacology , Shanxi Medical University , Taiyuan , China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: a Department of Endocrinology , the First Hospital of Shanxi Medical University, Shanxi Medical University , Taiyuan , China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: b Department of Pharmacology , Shanxi Medical University , Taiyuan , China.|c Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University , Taiyuan , China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D006706:Homeostasis / Q000187:drug effects / Q000502:physiology; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D026902:Potassium Channel Blockers / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology
chemical_list: D007328:Insulin; D026902:Potassium Channel Blockers; C017759:9-(tetrahydro-2-furyl)-adenine; D005576:Colforsin; D000242:Cyclic AMP; D000225:Adenine
keywords: action potential; cyclic AMP; insulin secretion; voltage-dependent potassium channels; β cells