title: Electrochemical studies of DNA interaction and antimicrobial activities of MnII, FeIII, CoIIand NiIISchiff base tetraazamacrocyclic complexes.
abstract: Tetraazamacrocyclic complexes of MnII, FeIII, CoIIand NiIIhave been synthesized by template method. These tetraazamacrocycles have been analyzed with various techniques like molar conductance, IR, UV-vis, mass spectral and cyclic voltammetric studies. On the basis of all these studies, octahedral geometry has been assigned to these tetraazamacrocyclic complexes. The DNA binding properties of these macrocyclic complexes have been investigated by electronic absorption spectra, fluorescence spectra, cyclic voltammetric and differential pulse voltammetric studies. The cyclic voltammetric data showed that ipcand ipawere effectively decreased in the presence of calf thymus DNA, which is a strong evidence for the interaction of these macrocyclic complexes with the calf thymus DNA (ct-DNA). The heterogeneous electron transfer rate constant found in the order: KCoII>KNiII>KMnIIwhich indicates that CoIImacrocyclic complex has formed a strong intercalated intermediate. The Stern-Volmer quenching constant (KSV) and voltammetric binding constant were found in the order KSV(CoII)>KSV(NiII)>KSV(MnII) and K+(CoII)>K+(NiII)>K+(MnII) which shows that CoIImacrocyclic complex exhibits the high interaction affinity towards ct-DNA by the intercalation binding. Biological studies of the macrocyclic complexes compared with the standard drug like Gentamycin, have shown antibacterial activities against E. coli, P. aeruginosa, B. cereus, S. aureus and antifungal activity against C. albicans.
authors.affiliation: Department of Chemistry, Gurukula Kangri University, Haridwar 249404, India. Electronic address: anujkumar9791@gmail.com.
authors.forename: Anuj
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Department of Chemistry, GLA University, Mathura, Uttar Pradesh 281406, India.
authors.forename: Vinod Kumar
authors.initials: VK
authors.lastname: Vashistha
authors.affiliation: Department of Chemistry, Gurukula Kangri University, Haridwar 249404, India.
authors.forename: Prashant
authors.initials: P
authors.lastname: Tevatia
authors.affiliation: Department of Chemistry, Gurukula Kangri University, Haridwar 249404, India.
authors.forename: Randhir
authors.initials: R
authors.lastname: Singh
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects; D002417:Cattle; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004058:Diffusion; D004563:Electrochemistry / Q000379:methods*; D004583:Electrons; D047028:Macrocyclic Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D012545:Schiff Bases / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence; D013055:Spectrophotometry, Infrared
chemical_list: D000890:Anti-Infective Agents; D056831:Coordination Complexes; D047028:Macrocyclic Compounds; D012545:Schiff Bases; D004247:DNA; C427203:calf thymus DNA
keywords: Antibacterial activity; Electrochemical studies; Fluorescence quenching; Schiff base