title: Fluorescence enhancement of oligodeoxynucleotides modified with green fluorescent protein chromophore mimics upon triplex formation.
abstract: Green fluorescent protein (GFP)-based molecular-rotor chromophores were attached to the 5-positions of deoxyuridines, and subsequently, incorporated into the middle positions of oligodeoxynucleotides. These oligonucleotides were designed to form triplex DNA in order to encapsulate the GFP chromophores, mimicking GFP structures. Upon triplex formation, the embedded GFP chromophores exhibited fluorescence enhancement, suggesting the potential application of these fluorescent probes for the detection of nucleic acids.
authors.affiliation: Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta-Cho, Midori-ku, Yokohama 226-8501, Japan. msekine@bio.titech.ac.jp kseio@bio.titech.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kanamori
authors.affiliation: Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta-Cho, Midori-ku, Yokohama 226-8501, Japan. msekine@bio.titech.ac.jp kseio@bio.titech.ac.jp.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Takamura
authors.affiliation: Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta-Cho, Midori-ku, Yokohama 226-8501, Japan. msekine@bio.titech.ac.jp kseio@bio.titech.ac.jp.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Tago
authors.affiliation: Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta-Cho, Midori-ku, Yokohama 226-8501, Japan. msekine@bio.titech.ac.jp kseio@bio.titech.ac.jp.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Masaki
authors.affiliation: Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta-Cho, Midori-ku, Yokohama 226-8501, Japan. msekine@bio.titech.ac.jp kseio@bio.titech.ac.jp.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ohkubo
authors.affiliation: Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta-Cho, Midori-ku, Yokohama 226-8501, Japan. msekine@bio.titech.ac.jp kseio@bio.titech.ac.jp.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sekine
authors.affiliation: Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta-Cho, Midori-ku, Yokohama 226-8501, Japan. msekine@bio.titech.ac.jp kseio@bio.titech.ac.jp.
authors.forename: Kohji
authors.identifier: http://orcid.org/0000-0001-5258-4763
authors.initials: K
authors.lastname: Seio
mesh_terms: D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*
chemical_list: D009838:Oligodeoxyribonucleotides; C051802:triplex DNA; D049452:Green Fluorescent Proteins; D004247:DNA