title: 1H NMR-Based Global Metabolic Studies of Pseudomonas aeruginosa upon Exposure of the Quorum Sensing Inhibitor Resveratrol.
abstract: Quorum sensing (QS) is a process of bacterial communication that has been a novel target for drug discovery. Pyocyanin quantification assay confirmed that resveratrol was an effective quorum sensing inhibitor (QSI) against Pseudomonas aeruginosa PAO1. In this study, the global metabolite changes of P. aeruginosa PAO1 exposed to QSI resveratrol were investigated by1H NMR spectroscopy. A total of 40 metabolites containing amino acids, organic acid, organic amine, and energy storage compounds were identified. The changed metabolic profile indicated that resveratrol influenced pathways including oxidative stress, protein synthesis, and energy metabolism. Oxidative stress could upregulate the expression of genes related to QS in P. aeruginosa. It suggested that resveratrol could inhibit the QS systems in P. aeruginosa PAO1 by relieving oxidative stress due to its antioxidant activity. On the other hand, resveratrol could attenuate the pathogenicity of P. aeruginosa PAO1 by disturbing the TCA cycle so that anaerobic respiration could suppress the virulence because anaerobiosis could induce the loss of cytotoxicity regulated by QS in P. aeruginosa. These findings deepened our comprehending of the metabolic responses of P. aeruginosa PAO1 to resveratrol and pinpointed the possible underlying mechanism of resveratrol's inhibition effect on QS in P. aeruginosa PAO1.
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology , Xiao Ling Wei No. 200, Nanjing 210094, China.
authors.forename: Tongtong
authors.initials: T
authors.lastname: Chen
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology , Xiao Ling Wei No. 200, Nanjing 210094, China.
authors.forename: Jiyang
authors.initials: J
authors.lastname: Sheng
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology , Xiao Ling Wei No. 200, Nanjing 210094, China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Fu
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology , Xiao Ling Wei No. 200, Nanjing 210094, China.
authors.forename: Minghui
authors.initials: M
authors.lastname: Li
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology , Xiao Ling Wei No. 200, Nanjing 210094, China.
authors.forename: Junsong
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology , Xiao Ling Wei No. 200, Nanjing 210094, China.|State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University , Haikou 570228, China.
authors.forename: Ai-Qun
authors.identifier: 0000-0002-8089-6200
authors.initials: AQ
authors.lastname: Jia
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D002952:Citric Acid Cycle / Q000187:drug effects; D015964:Gene Expression Regulation, Bacterial; D006019:Glycolysis / Q000187:drug effects; D053858:Metabolic Networks and Pathways / Q000187:drug effects / Q000235:genetics; D055432:Metabolomics; D019906:Nuclear Magnetic Resonance, Biomolecular; D018384:Oxidative Stress; D010936:Plant Extracts / Q000737:chemistry; D014176:Protein Biosynthesis / Q000187:drug effects; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D011710:Pyocyanine / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D053038:Quorum Sensing / Q000187:drug effects*; D000077185:Resveratrol; D032281:Smilax / Q000737:chemistry; D013267:Stilbenes / Q000302:isolation & purification / Q000494:pharmacology*; D037521:Virulence Factors / Q000037:antagonists & inhibitors / Q000096:biosynthesis
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D013267:Stilbenes; D037521:Virulence Factors; D011710:Pyocyanine; D000077185:Resveratrol
keywords: 1H NMR; Pseudomonas aeruginosa; metabolomics; quorum sensing; resveratrol