title: Assigning the Algal Source of Dimethylsulfide Using a Selective Lyase Inhibitor.
abstract: Atmospheric dimethylsulfide (DMS) is massively produced in the oceans by bacteria, algae, and corals. To enable identification of DMS sources, we developed a potent mechanism-based inhibitor of the algal Alma dimethylsulfoniopropionate lyase family that does not inhibit known bacterial lyases. Its application to coral holobiont indicates that DMS originates from Alma lyase(s). This biochemical profiling may complement meta-genomics and transcriptomics to provide better understanding of the marine sulfur cycle.
authors.affiliation: Department of Biomolecular Sciences and ‡Department of Plant and Environmental Sciences, Weizmann Institute of Science , Rehovot, Israel.
authors.forename: Uria
authors.initials: U
authors.lastname: Alcolombri
authors.affiliation: Department of Biomolecular Sciences and ‡Department of Plant and Environmental Sciences, Weizmann Institute of Science , Rehovot, Israel.
authors.forename: Lei
authors.initials: L
authors.lastname: Lei
authors.affiliation: Department of Biomolecular Sciences and ‡Department of Plant and Environmental Sciences, Weizmann Institute of Science , Rehovot, Israel.
authors.forename: Diana
authors.initials: D
authors.lastname: Meltzer
authors.affiliation: Department of Biomolecular Sciences and ‡Department of Plant and Environmental Sciences, Weizmann Institute of Science , Rehovot, Israel.
authors.forename: Assaf
authors.initials: A
authors.lastname: Vardi
authors.affiliation: Department of Biomolecular Sciences and ‡Department of Plant and Environmental Sciences, Weizmann Institute of Science , Rehovot, Israel.
authors.forename: Dan S
authors.identifier: 0000-0002-5914-8240
authors.initials: DS
authors.lastname: Tawfik
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D001419:Bacteria / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D013437:Carbon-Sulfur Lyases / Q000037:antagonists & inhibitors / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000378:metabolism; D058087:Haptophyta / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D009792:Oceans and Seas; D013440:Sulfides / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D013440:Sulfides; D013437:Carbon-Sulfur Lyases; C400924:dimethylsulfoniopropionate lyase; C004784:dimethyl sulfide