title: Elevated expression of serine/threonine phosphatase type 5 correlates with malignant proliferation in human osteosarcoma.
abstract: Osteosarcoma is the most common primary malignant bone tumor in adolescents and young adults. However, the involvement of serine/threonine phosphatase type 5 (PP5) in osteosarcoma remains unclear. The aim of this study was to evaluate the functional role of PP5 in osteosarcoma cells. Firstly, we found that PP5 is widely expressed in several human osteosarcoma cell lines. Then we used lentivirus-delivered siRNA to silence PP5 expression in Saos-2 and U2OS cell lines. Knockdown of endogenous PP5 expression by shRNA-expressing lentivirus significantly decreased the viability and proliferation of the osteosarcoma cells. Moreover, FACS analysis showed that knockdown of PP5 expression induced a significant arrest in the G0/G1 phase of the cell cycle, which was associated with the inhibition of cell proliferation. Therefore, knockdown of PP5 is likely to provide a novel alternative to targeted therapy of osteosarcoma and deserves further investigation.
authors.affiliation: Department of Medical Oncology, The Affiliated 6th People's Hospital of Shanghai Jiaotong University, Shanghai 200223, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Han
authors.affiliation: Department of Medical Oncology, The Affiliated 6th People's Hospital of Shanghai Jiaotong University, Shanghai 200223, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Gan
authors.affiliation: Department of Medical Oncology, The Affiliated 6th People's Hospital of Shanghai Jiaotong University, Shanghai 200223, China.
authors.forename: Shuchen
authors.initials: S
authors.lastname: Lin
authors.affiliation: Department of Medical Oncology, The Affiliated 6th People's Hospital of Shanghai Jiaotong University, Shanghai 200223, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Hu
authors.affiliation: Department of Medical Oncology, The Affiliated 6th People's Hospital of Shanghai Jiaotong University, Shanghai 200223, China.
authors.forename: Zan
authors.initials: Z
authors.lastname: Shen
authors.affiliation: Department of Medical Oncology, The Affiliated 6th People's Hospital of Shanghai Jiaotong University, Shanghai 200223, China.
authors.forename: Daliu
authors.initials: D
authors.lastname: Min
mesh_terms: D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects* / Q000235:genetics; D020868:Gene Silencing / Q000187:drug effects; D006801:Humans; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D012516:Osteosarcoma / Q000188:drug therapy / Q000201:enzymology / Q000473:pathology*; D010749:Phosphoprotein Phosphatases / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D034741:RNA, Small Interfering / Q000494:pharmacology; D016192:Resting Phase, Cell Cycle; D015854:Up-Regulation
chemical_list: D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D010749:Phosphoprotein Phosphatases; C089641:protein phosphatase 5
keywords: Osteosarcoma; PP5; cell proliferation; lentivirus; siRNA; targeted therapy