title: Crystal structure of PvdO from Pseudomonas aeruginosa.
abstract: Pyoverdine I (PVDI) is a water-soluble fluorescein siderophore with strong iron chelating ability from the gram-negative pathogen Pseudomonas aeruginosa PAO1. Compared to common siderophores, PVDI is a relatively large compound whose synthesis requires a group of enzymes with different catalytic activities. In addition to four nonribosomal peptide synthetases (NRPS) which are responsible for the production of the peptide backbone of PVDI, several additional enzymes are associated with the modification of the side chains. PvdO is one of these enzymes and participates in PVDI precursor maturation in the periplasm. We determined the crystal structure of PvdO at 1.24 Å resolution. The PvdO structure shares a common fold with some FGly-generating enzymes (FGE) and is stabilized by Ca2+. However, the catalytic residues in FGE are not observed in PvdO, indicating PvdO adopts a unique catalytic mechanism.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
authors.forename: Zenglin
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
authors.forename: Guohui
authors.initials: G
authors.lastname: Bai
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Hengchuan
authors.initials: H
authors.lastname: Xia
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
authors.forename: Lichuan
authors.initials: L
authors.lastname: Gu
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China. Electronic address: xu_sujuan@sdu.edu.cn.
authors.forename: Sujuan
authors.initials: S
authors.lastname: Xu
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D002118:Calcium / Q000737:chemistry; D002152:Calorimetry, Differential Scanning; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D010453:Peptide Synthases / Q000737:chemistry*; D011487:Protein Conformation; D011550:Pseudomonas aeruginosa / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D010453:Peptide Synthases; C091136:pyoverdine synthetase protein, Pseudomonas; D002118:Calcium
keywords: Crystal structure; FGE; Pseudomonas aeruginosa; PvdO