title: Ionic-liquid pretreatment of cassava residues for the cogeneration of fermentative hydrogen and methane.
abstract: An ionic liquid of N-methylmorpholine-N-oxide (NMMO) was used to effectively pretreat cassava residues for the efficient enzymatical hydrolysis and cogeneration of fermentative hydrogen and methane. The reducing sugar yield of enzymolysed cassava residues with NMMO pretreatment improved from 36 to 42g/100g cassava residues. Scanning electron microscopy images revealed the formation of deep grooves (∼4μm wide) and numerous pores in the cassava residues pretreated with NMMO. X-ray diffraction patterns showed that the crystallinity coefficient of NMMO-pretreated cassava residues decreased from 40 to 34. Fourier transform infrared spectra indicated that crystal cellulose I was partially transformed to amorphous cellulose II in the NMMO-pretreated cassava residues. This transformation resulted in a reduced crystallinity index from 0.85 to 0.77. Hydrogen yield from the enzymolysed cassava residues pretreated with NMMO increased from 92.3 to 126mL/gTVS, and the sequential methane yield correspondingly increased from 79.4 to 101.6mL/g TVS.
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China. Electronic address: juncheng@zju.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Jiabei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Richen
authors.initials: R
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Liu
authors.affiliation: Shandong Botong New Energy Company, Jining 272000, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Kefa
authors.initials: K
authors.lastname: Cen
mesh_terms: D050260:Carbohydrate Metabolism / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003497:Cyclic N-Oxides / Q000494:pharmacology*; D003624:Darkness; D005285:Fermentation / Q000187:drug effects*; D006859:Hydrogen / Q000378:metabolism*; D052578:Ionic Liquids / Q000494:pharmacology*; D002365:Manihot / Q000737:chemistry* / Q000187:drug effects / Q000648:ultrastructure; D008697:Methane / Q000096:biosynthesis*; D009025:Morpholines / Q000494:pharmacology*; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D003497:Cyclic N-Oxides; D052578:Ionic Liquids; D009025:Morpholines; D006859:Hydrogen; C067960:4-methylmorpholine N-oxide; D008697:Methane
keywords: Cassava residues; Fermentation; Hydrogen; Ionic liquid; Methane