title: Investigation of diethylstilbestrol residue level in human urine samples by a specific monoclonal antibody.
abstract: Diethylstilbestrol (DES) is used as a kind of animal feed additive and affects people's health through the food chain. The purpose of this study is to detect the residue level of DES in 576 human urine samples directly. DES-BSA was used to immunize Balb/c mice. The monoclonal antibody was produced by hybridoma that was screened through cell fusion techniques. Finally, we developed the indirect competitive ELISA method to analyze 576 human urine samples from Zhejiang Province, China. The IC50of this method was 3.33 ng/mL. The LOD and LOQ were 0.16 and 0.54 ng/mL. Linear range of the standard curve was from LOD to 12.50 ng/mL. There was no cross-reactivity with two kinds of estrogens and two structural analogs with DES. Five hundred seventy-six urine samples were analyzed by the indirect competitive ELISA method, and the detection rate was 98.78%. The mean concentration and geometric mean were 4.70 and 3.50 ng/mL. The indirect competitive ELISA method based on monoclonal antibody was sensitive and reliable for the detection of DES in human urine samples. The results warned us to pay more attention to human health and food safety.
authors.affiliation: Institute of Drug Metabolism and Pharmaceutical Analysis, College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang Road, Zijingang Campus, Hangzhou, Zhejiang, 310058, China.
authors.forename: Dai
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Hangzhou EPIE Bio-detection Technology Limited, Hangzhou, 310051, China.
authors.forename: Lifang
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Hangzhou EPIE Bio-detection Technology Limited, Hangzhou, 310051, China.
authors.forename: Yajing
authors.initials: Y
authors.lastname: Lei
authors.affiliation: Institute of Drug Metabolism and Pharmaceutical Analysis, College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang Road, Zijingang Campus, Hangzhou, Zhejiang, 310058, China.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Institute of Drug Metabolism and Pharmaceutical Analysis, College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang Road, Zijingang Campus, Hangzhou, Zhejiang, 310058, China. zhoujie127@zju.edu.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Institute of Drug Metabolism and Pharmaceutical Analysis, College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang Road, Zijingang Campus, Hangzhou, Zhejiang, 310058, China. chenshuqing@zju.edu.cn.
authors.forename: Shuqing
authors.initials: S
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D004054:Diethylstilbestrol / Q000276:immunology / Q000652:urine*; D004350:Drug Residues / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D057230:Limit of Detection; D008297:Male; D051379:Mice; D008875:Middle Aged; D016482:Urinalysis / Q000379:methods*; D055815:Young Adult
chemical_list: D000911:Antibodies, Monoclonal; D004054:Diethylstilbestrol
keywords: Diethylstilbestrol; ELISA; Human health; Human urine samples; Monoclonal antibody; Residue level