title: In vivo modulation of LPS induced leukotrienes generation and oxidative stress by sesame lignans.
abstract: The role of inflammation and oxidative stress is critical during onset of metabolic disorders and this has been sufficiently established in literature. In the present study, we evaluated the effects of sesamol and sesamin, two important bioactive molecules present in sesame oil, on the generation of inflammatory and oxidative stress factors in LPS injected rats. Sesamol and sesamin lowered LPS induced expression of cPLA2(61 and 56%), 5-LOX (44 and 51%), BLT-1(32 and 35%) and LTC4synthase (49 and 50%), respectively, in liver homogenate. The diminished serum LTB4(53 and 64%) and LTC4(67 and 44%) levels in sesamol and sesamin administered groups, respectively, were found to be concurrent with the observed decrease in the expression of cPLA2and 5-LOX. The serum levels of TNF-α (29 and 19%), MCP-1 (44 and 57%) and IL-1β (43 and 42%) were found to be reduced in sesamol and sesamin group, respectively, as given in parentheses, compared to LPS group. Sesamol and sesamin offered protection against LPS induced lipid peroxidation in both serum and liver. Sesamol, but not sesamin, significantly restored the loss of catalase and glutathione reductase activity due to LPS (P<.05). However, both sesamol and sesamin reverted SOD activities by 92 and 98%, respectively. Thus, oral supplementation of sesamol and sesamin beneficially modulated the inflammatory and oxidative stress markers, as observed in the present study, in LPS injected rats. Our report further advocates the potential use of sesamol and sesamin as an adjunct therapy wherein, inflammatory and oxidative stress is of major concern.
authors.affiliation: Department of Protein Chemistry & Technology, CSIR-Central Food Technological Research Institute, Mysuru, 570020, India.
authors.forename: Puttaraju Srikantamurthy
authors.initials: PS
authors.lastname: Yashaswini
authors.affiliation: Department of Biochemistry, CSIR- Central Food Technological Research Institute, Mysuru, 570020, India.
authors.forename: Bettadahalli
authors.initials: B
authors.lastname: Sadashivaiah
authors.affiliation: Department of Biochemistry, CSIR- Central Food Technological Research Institute, Mysuru, 570020, India.
authors.forename: Talahalli Ravichandra
authors.initials: TR
authors.lastname: Ramaprasad
authors.affiliation: Department of Protein Chemistry & Technology, CSIR-Central Food Technological Research Institute, Mysuru, 570020, India. Electronic address: sridevi@cftri.res.in.
authors.forename: Sridevi Annapurna
authors.initials: SA
authors.lastname: Singh
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000378:metabolism / Q000627:therapeutic use*; D000975:Antioxidants / Q000302:isolation & purification / Q000378:metabolism / Q000627:therapeutic use; D001094:Arachidonate 5-Lipoxygenase / Q000737:chemistry / Q000378:metabolism; D052117:Benzodioxoles / Q000378:metabolism / Q000627:therapeutic use*; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D019587:Dietary Supplements; D004149:Dioxoles / Q000302:isolation & purification / Q000378:metabolism / Q000627:therapeutic use*; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D006505:Hepatitis / Q000209:etiology / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control*; D018836:Inflammation Mediators / Q000097:blood / Q000378:metabolism; D020024:Leukotriene Antagonists / Q000302:isolation & purification / Q000378:metabolism / Q000627:therapeutic use; D015289:Leukotrienes / Q000819:agonists / Q000097:blood / Q000378:metabolism*; D017705:Lignans / Q000302:isolation & purification / Q000378:metabolism / Q000627:therapeutic use*; D015227:Lipid Peroxidation / Q000187:drug effects; D008070:Lipopolysaccharides / Q000633:toxicity; D008099:Liver / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000378:metabolism / Q000627:therapeutic use*; D054510:Phospholipases A2, Cytosolic / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D017208:Rats, Wistar; D018102:Receptors, Leukotriene B4 / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism; D012715:Sesame Oil / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D052117:Benzodioxoles; D015415:Biomarkers; D004149:Dioxoles; D018836:Inflammation Mediators; D020024:Leukotriene Antagonists; D015289:Leukotrienes; D017705:Lignans; D008070:Lipopolysaccharides; D010636:Phenols; D018102:Receptors, Leukotriene B4; D012715:Sesame Oil; C025583:sesamol; D001094:Arachidonate 5-Lipoxygenase; D005982:Glutathione Transferase; D054510:Phospholipases A2, Cytosolic; C041116:leukotriene-C4 synthase; C054125:sesamin
keywords: Cytokines; Inflammation; LPS; Leukotrienes; Sesamin; Sesamol