title: Chitosan Modulates Inflammatory Responses in Rats Infected with EnterotoxigenicEscherichia coli.
abstract: This study aims to investigate the effects of dietary chitosan (COS) on gastrointestinal pathogen resistance in mice model. For two weeks, a control group of ICR mice received a basal diet whilst the intervention group received the basal diet supplemented with 300 mg/kg COS. After two weeks, the mice fed the supplemented diet had a lower body weight. Then enterotoxigenicEscherichia coli(E. coli) was administered to the mice through oral gavage, with each mouse receiving 108CFU. At day 7 after infection, the bacterial load in the jejunum and faeces was significantly lower in the COS group than that in the control group. Moreover, the mRNA and protein levels of IL-1β, IL-6, IL-17, IL-18, and TNF-αwere significantly lower in the group of mice receiving the COS diet; also the jejunal production of toll-like receptor-4 (TLR-4) was suppressed in the COS group. These results indicate the intervention influenced inflammation and controlledE. coliinfection.
authors.affiliation: College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan 410128, China; Institute of Subtropical Agriculture, Chinese Academy of Sciences, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-Ecological Processes in Subtropical Region, Changsha, Hunan 410125, China.
authors.forename: Gang
authors.identifier: 0000-0002-9475-5134
authors.initials: G
authors.lastname: Liu
authors.affiliation: Institute of Subtropical Agriculture, Chinese Academy of Sciences, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-Ecological Processes in Subtropical Region, Changsha, Hunan 410125, China.
authors.forename: Shuai
authors.identifier: 0000-0002-9337-6191
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan 410128, China.
authors.forename: Guiping
authors.identifier: 0000-0002-6850-9626
authors.initials: G
authors.lastname: Guan
authors.affiliation: College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan 410128, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Tan
authors.affiliation: Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
authors.forename: Naif A
authors.identifier: 0000-0002-2663-6328
authors.initials: NA
authors.lastname: Al-Dhabi
authors.affiliation: Hunan Institute of Animal and Veterinary Science, Changsha, Hunan 410131, China.
authors.forename: Hongbing
authors.identifier: 0000-0002-7362-7854
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
authors.forename: Veeramuthu
authors.identifier: 0000-0002-4136-2003
authors.initials: V
authors.lastname: Duraipandiyan
authors.affiliation: College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan 410128, China.
authors.forename: Jun
authors.identifier: 0000-0001-9532-8444
authors.initials: J
authors.lastname: Fang
mesh_terms: D000818:Animals; D058491:Bacterial Load; D001835:Body Weight / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D016207:Cytokines / Q000378:metabolism; D004032:Diet; D004731:Endotoxins / Q000378:metabolism; D054307:Enterotoxigenic Escherichia coli; D004927:Escherichia coli Infections / Q000276:immunology* / Q000473:pathology; D007249:Inflammation; D051379:Mice; D008813:Mice, Inbred ICR; D012333:RNA, Messenger / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000378:metabolism
chemical_list: D016207:Cytokines; D004731:Endotoxins; D012333:RNA, Messenger; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D048271:Chitosan