title: Setting up the cut-off level of a sensitive barcode lateral flow assay with magnetic nanoparticles.
abstract: A barcode lateral flow immunoassay (LFIA) based on magnetic nanoparticles with a controllable cut-off level was developed for the first time. The regulation of the cut-off levels was based on the changes in the position and concentration of three antibodies (two monoclonal and polyclonal) with different affinities (KD=4.22×10-9, 9.67×10-9, 1.05×10-8M), respectively. To obtain specific conjugates, monoclonal antibodies were covalently immobilized on the magnetic nanoparticles' surface. Potato virus X causing a reduction in the potato yield was used as a model polyvalent antigen. To detect potato virus X in the leaf extracts, a barcode LFIA with cut-off levels of 3, 30, and 150ng/mL was used for the analysis. The application of magnetic concentration leads to a six-fold reduction in the first cut-off level (0.5ng/mL) in comparison with magnetic LFIA without the concentration stage.
authors.affiliation: A.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, Russia.
authors.forename: Vasily G
authors.initials: VG
authors.lastname: Panferov
authors.affiliation: A.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, Russia.
authors.forename: Irina V
authors.initials: IV
authors.lastname: Safenkova
authors.affiliation: A.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, Russia.
authors.forename: Anatoly V
authors.initials: AV
authors.lastname: Zherdev
authors.affiliation: A.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, Russia. Electronic address: dzantiev@inbi.ras.ru.
authors.forename: Boris B
authors.initials: BB
authors.lastname: Dzantiev
keywords: Cut-off regulation; Immunoassay; Lateral flow assay; Magnetic nanoparticles; Potato virus x