title: Isoform-Specific Phosphorylation in Human Hsp90β Affects Interaction with Clients and the Cochaperone Cdc37.
abstract: The 90-kDa heat shock proteins (Hsp90s) assist the maturation of many key regulators of signal transduction pathways and cellular control circuits like protein kinases and transcription factors and chaperone their stability and activity. In this function, Hsp90s cooperate with some 30 cochaperones and they are themselves subject to regulation by numerous post-translational modifications. In vertebrates, two major isoforms exist in the cytosol, Hsp90α and Hsp90β, which share a high degree of sequence identity and are expressed in tissue- and environmental condition-dependent manner. We identified an isoform-specific phosphorylation site in human Hsp90β. This phosphorylation site seems to be linked to vertebrate evolution since it is not found in invertebrata but in all tetrapoda and many but not all fish species. We provide data suggesting that this phosphorylation is important for the activation of Hsp90 clients like glucocorticoid receptor and a protein kinase. Replacement of the phosphorylation site by glutamate affects the conformational dynamics of Hsp90 and interaction with the kinase-specific cochaperone Cdc37.
authors.affiliation: Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH-Alliance, D-69120, Heidelberg, Germany.
authors.forename: Minh T N
authors.initials: MTN
authors.lastname: Nguyen
authors.affiliation: Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH-Alliance, D-69120, Heidelberg, Germany.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Knieß
authors.affiliation: Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH-Alliance, D-69120, Heidelberg, Germany.
authors.forename: Soumya
authors.initials: S
authors.lastname: Daturpalli
authors.affiliation: Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH-Alliance, D-69120, Heidelberg, Germany.
authors.forename: Laura
authors.initials: L
authors.lastname: Le Breton
authors.affiliation: School of Public Health, Southern Medical University, Guangzhou, 510515, Guangdong, China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Ke
authors.affiliation: School of Public Health, Southern Medical University, Guangzhou, 510515, Guangdong, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Chen
authors.affiliation: Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH-Alliance, D-69120, Heidelberg, Germany. Electronic address: m.mayer@zmbh.uni-heidelberg.de.
authors.forename: Matthias P
authors.initials: MP
authors.lastname: Mayer
mesh_terms: D000595:Amino Acid Sequence; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D018833:Chaperonins / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D010766:Phosphorylation; D011487:Protein Conformation; D020033:Protein Isoforms; D011494:Protein Kinases / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D011965:Receptors, Glucocorticoid / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism
chemical_list: C498362:CDC37 protein, human; D018797:Cell Cycle Proteins; D018841:HSP90 Heat-Shock Proteins; D020033:Protein Isoforms; D011965:Receptors, Glucocorticoid; D014157:Transcription Factors; D011494:Protein Kinases; D018833:Chaperonins
keywords: Hsp90; evolution; molecular chaperone; phosphorylation; post-translational modification