title: Oxidative cleavage and hydrolytic boosting of cellulose in soybean spent flakes by Trichoderma reesei Cel61A lytic polysaccharide monooxygenase.
abstract: The auxiliary activity family 9 (AA9) copper-dependent lytic polysaccharide monooxygenase (LPMO) from Trichoderma reesei (EG4; TrCel61A) was investigated for its ability to oxidize the complex polysaccharides from soybean. The substrate specificity of the enzyme was assessed against a variety of substrates, including both soy spent flake, a by-product of the soy food industry, and soy spent flake pretreated with sodium hydroxide. Products from enzymatic treatments were analyzed using mass spectrometry and high performance anion exchange chromatography. We demonstrate that TrCel61A is capable of oxidizing cellulose from both pretreated soy spent flake and phosphoric acid swollen cellulose, oxidizing at both the C1 and C4 positions. In addition, we show that the oxidative activity of TrCel61A displays a synergistic effect capable of boosting endoglucanase activity, and thereby substrate depolymerization of soy cellulose, by 27%.
authors.affiliation: DuPont™ Nutrition Biosciences ApS, Edwin Rahrs Vej 38, Brabrand, 8220, Denmark; Department of Chemical and Biochemical Engineering, Center for Bioprocess Engineering, Technical University of Denmark, Søltofts Plads, Building 229, Kgs. Lyngby 2800, Denmark. Electronic address: brian.pierce@dupont.com.
authors.forename: Brian C
authors.initials: BC
authors.lastname: Pierce
authors.affiliation: Department of Chemical and Biochemical Engineering, Center for Bioprocess Engineering, Technical University of Denmark, Søltofts Plads, Building 229, Kgs. Lyngby 2800, Denmark. Electronic address: jaag@kt.dtu.dk.
authors.forename: Jane Wittrup
authors.initials: JW
authors.lastname: Agger
authors.affiliation: DuPont™ Nutrition Biosciences ApS, Edwin Rahrs Vej 38, Brabrand, 8220, Denmark. Electronic address: jesper.wichmann@dupont.com.
authors.forename: Jesper
authors.initials: J
authors.lastname: Wichmann
authors.affiliation: Department of Chemical and Biochemical Engineering, Center for Bioprocess Engineering, Technical University of Denmark, Søltofts Plads, Building 229, Kgs. Lyngby 2800, Denmark. Electronic address: am@kt.dtu.dk.
authors.forename: Anne S
authors.initials: AS
authors.lastname: Meyer
mesh_terms: D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D005656:Fungal Proteins / Q000378:metabolism*; D006868:Hydrolysis; D013058:Mass Spectrometry; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010084:Oxidation-Reduction; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D013025:Glycine max / Q000737:chemistry; D013379:Substrate Specificity; D014242:Trichoderma / Q000201:enzymology*
chemical_list: D005656:Fungal Proteins; D011134:Polysaccharides; D002482:Cellulose; D006899:Mixed Function Oxygenases; D002480:Cellulase
keywords: Cellulose hydrolysis; Lytic polysaccharide monooxygenase; Soy polysaccharides; Soy spent flake; Trichoderma reesei Cel61A (TrCel61A)