title: A study on the GC-MS analysis of bioactive components and pancreato-protective effect of methanolic extract of Brassica oleracea L. var. botrytis.
abstract: The ever-increasing problem of pancreatitis due to alcohol abuse demands evaluation of novel drugs of plant origin. This study explores the therapeutic effects of the methanolic extract of Brassica oleraceae (MEBO) on ethanol and cerulein induced pancreatitis in rats. The MEBO was subjected to GC-MS and HPLC analysis. Male albino Wistar rats were divided into various groups, fed with alcohol (36% of total calories for 5 weeks) and cerulein (20 μg/kg b.wt i.p, weekly thrice for last three weeks) with or without MEBO (40 mg/kg b.wt). Serum lipase, amylase, IL-1β, IL-18, caspase-1, lipid peroxides, oxidative stress index and antioxidant status were assessed in pancreas. Six compounds were identified in GC-MS analysis. Co-administration of MEBO reduced the pancreatic marker enzymes in serum, IL-1β, IL-18 and caspase-1 and increased the antioxidant status of pancreas. The pancreato-protective effect of Brassica oleraceae may be attributed to well-known anti-inflammatory flavonoids, luteolin, quercetin and myricetin.
authors.affiliation: a Department of Biochemistry Bharathi Women's College (Affiliated to University of Madras) , Chennai , Tamil Nadu , India.
authors.forename: Sadanandan
authors.initials: S
authors.lastname: Rajapriya
authors.affiliation: a Department of Biochemistry Bharathi Women's College (Affiliated to University of Madras) , Chennai , Tamil Nadu , India.
authors.forename: Arumugam
authors.initials: A
authors.lastname: Geetha
authors.affiliation: b Department of Biochemistry, School of Life Sciences , Vels University , Chennai , Tamil Nadu , India.
authors.forename: Kavasseri
authors.initials: K
authors.lastname: Ganesan Kripa
mesh_terms: D000681:Amylases / Q000378:metabolism; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001937:Brassica / Q000737:chemistry*; D002108:Ceruletide / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000009:adverse effects; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D000432:Methanol / Q000737:chemistry; D018384:Oxidative Stress / Q000187:drug effects; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism; D010195:Pancreatitis / Q000139:chemically induced / Q000188:drug therapy*; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D017208:Rats, Wistar
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D015415:Biomarkers; D010936:Plant Extracts; D020011:Protective Agents; D000431:Ethanol; D002108:Ceruletide; D000681:Amylases; D000432:Methanol
keywords: Brassica oleraceae; GC-MS; antioxidants; ethanol; interleukins; lipase-amylase; pancreatitis