title: 2-Chloroethanol Induced Upregulation of Matrix Metalloproteinase-2 in Primary Cultured Rat Astrocytes Via MAPK Signal Pathways.
abstract: This study was to explore the mechanisms underlying 1,2-dichloroethane (1,2-DCE) induced brain edema by focusing on alteration of matrix metalloproteinase-2 (MMP-2) in rat astrocytes induced by 2-chloroethanol (2-CE), an intermediate metabolite of 1,2-DCEin vivo. Protein and mRNA levels of MMP-2, and the phosphorylated protein levels of p38 MAPK (p-p38), extracellular signal regulated protein kinase (p-ERK1/2) and c-Jun N-terminal kinase (p-JNK1/2) in astrocytes were examined by immunostaining, western blot or real-time RT-PCR analysis. Findings from this study disclosed that protein levels of MMP-2 were upregulated by 2-CE in astrocytes. Meanwhile, protein levels of p-p38, p-ERK1/2 and p-JNK1/2 were also increased apparently in the cells treated with 2-CE. Moreover, pretreatment of astrocytes with SB202190 (inhibitor of p38 MAPK), U0126 (inhibitor of ERK1/2) or SP600125 (inhibitor of JNK1/2) could suppress the upregulated expression of p-p38, p-ERK1/2, and p-JNK1/2. In response to suppressed protein levels of p-p38 and p-JNK1/2, the protein levels of MMP-2 also decreased significantly, indicating that activation of MAPK signal pathways were involved in the mechanisms underlying 2-CE-induced upregulation of MMP-2 expression.
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, China Medical University Shenyang, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Department of Physiology, China Medical University Shenyang, China.
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Liao
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, China Medical University Shenyang, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, China Medical University Shenyang, China.
authors.forename: Hongge
authors.initials: H
authors.lastname: Tang
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, China Medical University Shenyang, China.
authors.forename: Gaoyang
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, China Medical University Shenyang, China.
authors.forename: Fenghong
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, China Medical University Shenyang, China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Jin
keywords: 1,2-dichloroethane; 2-chloroethanol; astrocytes; matrix metalloproteinase-2; mitogen-activated protein kinase signal pathways