title: Fibrin improves skin wound perfusion in a diabetic rat model.
abstract: The fibrin matrix of the thrombus that is formed directly after wounding, is an important determinant of the success of the early phase of wound healing. This phase is often impaired in patients with diabetes. A promising approach to improve skin wound healing is the application of a pro-angiogenic fibrin matrix onto the wound. We studied this in 59 female WAG/RijCrl diabetic rats, in which we created two dorsal full-thickness wounds of which one was treated with a human physiological fibrin matrix (2mg/ml) and one with PBS as control. Wound healing parameters were determined at different time points. The wound closure was significantly improved in fibrin-treated wounds on day 3 and 7. Also, fibrin-treated wounds showed a significantly higher perfusion on day 28 and 35 compared to control wounds (p<0.05). CD68 staining revealed that human fibrin did not induce an immune response.
  
  
  CONCLUSIONS
  the application of a fibrin matrix on a diabetic wound showed improved perfusion and an increased early closure rate of the wound area.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Erasmus MC, University Medical Center, Rotterdam, The Netherlands; Department of Hematology, Erasmus MC, University Medical Center, Rotterdam, The Netherlands.
authors.forename: Tamara
authors.initials: T
authors.lastname: Hoppenbrouwers
authors.affiliation: Department of Plastic and Reconstructive Surgery, Erasmus MC, University Medical Center, Rotterdam, The Netherlands.
authors.forename: Bastiaan
authors.initials: B
authors.lastname: Tuk
authors.affiliation: Department of Plastic and Reconstructive Surgery, Erasmus MC, University Medical Center, Rotterdam, The Netherlands.
authors.forename: Esther M G
authors.initials: EM
authors.lastname: Fijneman
authors.affiliation: Department of Hematology, Erasmus MC, University Medical Center, Rotterdam, The Netherlands.
authors.forename: Moniek P M
authors.initials: MP
authors.lastname: de Maat
authors.affiliation: Department of Plastic and Reconstructive Surgery, Erasmus MC, University Medical Center, Rotterdam, The Netherlands. Electronic address: j.vanneck@erasmusmc.nl.
authors.forename: Johan W
authors.initials: JW
authors.lastname: van Neck
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000150:complications* / Q000473:pathology; D005260:Female; D005337:Fibrin / Q000627:therapeutic use*; D006801:Humans; D051381:Rats; D012039:Regional Blood Flow / Q000187:drug effects; D012867:Skin / Q000098:blood supply* / Q000187:drug effects* / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects*; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D005337:Fibrin; C067980:CD68 antigen, human; D000097382:CD68 Molecule
keywords: Diabetes; Fibrin; Perfusion; Wound healing