title: LPS promotes Th2 dependent sensitisation leading to anaphylaxis in a Pru p 3 mouse model.
abstract: Pru p 3 is the major peach allergen in the Mediterranean area. It frequently elicits severe reactions, limiting its study in humans, raising the need for animal models to investigate the immunological mechanisms involved. However, no anaphylaxis model exists for Pru p 3. We aimed to develop a model of peach anaphylaxis by sensitising mice with Pru p 3 in combination with lipopolysaccharide (LPS) as an adjuvant. Four groups of mice were sensitised intranasally: untreated; treated with Pru p 3; treated with LPS; treated with Pru p 3 + LPS. After sensitisation mice were intraperitoneally challenged with Pru p 3 and in vivo and in vitro parameters were evaluated. Only mice in the Pru p 3 + LPS group showed anaphylaxis symptoms, including a decrease in temperature. Determination of in vitro parameters showed a Th2 response with an increase of Pru p 3-specific IgE and IgG1. Moreover, at the cellular level, we found increased levels of IgE and IgG1 secreting Pru p 3-specific cells and a proliferative CD4+T-cell response. These results demonstrate that Pru p 3-specific anaphylaxis can be generated after nasal sensitisation to Pru p 3 in combination with LPS. This is a promising model for evaluating food allergy immunotherapies.
authors.affiliation: Research Laboratory, IBIMA, Regional University Hospital of Malaga, UMA, Malaga, Spain.
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Rodriguez
authors.affiliation: Research Laboratory, IBIMA, Regional University Hospital of Malaga, UMA, Malaga, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Aranda
authors.affiliation: Research Laboratory, IBIMA, Regional University Hospital of Malaga, UMA, Malaga, Spain.
authors.forename: Tahia D
authors.initials: TD
authors.lastname: Fernandez
authors.affiliation: Center for Plant Biotechnology and Genomic (UPM-INIA), Madrid, Spain.
authors.forename: Nuria
authors.initials: N
authors.lastname: Cubells-Baeza
authors.affiliation: Allergy Unit, IBIMA, Regional University Hospital of Malaga, UMA, Malaga, Spain.
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Torres
authors.affiliation: Allergy Unit, IBIMA, Regional University Hospital of Malaga, UMA, Malaga, Spain.
authors.forename: Francisca
authors.initials: F
authors.lastname: Gomez
authors.affiliation: Research Laboratory, IBIMA, Regional University Hospital of Malaga, UMA, Malaga, Spain.
authors.forename: Francisca
authors.initials: F
authors.lastname: Palomares
authors.affiliation: Research Laboratory, IBIMA, Regional University Hospital of Malaga, UMA, Malaga, Spain.
authors.forename: James R
authors.initials: JR
authors.lastname: Perkins
authors.affiliation: Glycosystems Laboratory, Instituto de Investigaciones Químicas (IIQ), CSIC - Universidad de Sevilla, Sevilla, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Rojo
authors.affiliation: Center for Plant Biotechnology and Genomic (UPM-INIA), Madrid, Spain.
authors.forename: Araceli
authors.initials: A
authors.lastname: Diaz-Perales
authors.affiliation: Research Laboratory, IBIMA, Regional University Hospital of Malaga, UMA, Malaga, Spain.|Allergy Unit, IBIMA, Regional University Hospital of Malaga, UMA, Malaga, Spain.
authors.forename: Cristobalina
authors.initials: C
authors.lastname: Mayorga
mesh_terms: D000707:Anaphylaxis / Q000276:immunology* / Q000473:pathology; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D052179:Antigens, Plant / Q000276:immunology; D049109:Cell Proliferation; D004195:Disease Models, Animal; D058501:Enzyme-Linked Immunospot Assay; D005260:Female; D007114:Immunization; D007073:Immunoglobulin E / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008070:Lipopolysaccharides; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000473:pathology
chemical_list: D000906:Antibodies; D052179:Antigens, Plant; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D007073:Immunoglobulin E