title: Microsatellite markers forNuphar japonica(Nymphaeaceae), an aquatic plant in the agricultural ecosystem of Japan.
abstract: OBJECTIVE
  Nupharspecies (Nymphaeaceae) are representative aquatic plants in irrigation ponds in Japanese agricultural ecosystems. We developed 15 polymorphic microsatellite markers forN. japonicaand confirmed their utility for its close relativesN. oguraensisvar.akiensisandN. ×saijoensis, which originated from natural hybridization betweenN. japonicaandN. oguraensis.
  
  
  RESULTS
  Genetic variation was characterized in 15 polymorphic loci in three populations ofN. japonica. The average number of alleles per locus was 3.47 (range = 2-9;n= 32), and the average expected heterozygosity per locus was 0.84 (range = 0.5-1.0); 11 loci were amplified inN. oguraensisvar.akiensisand 15 inN. ×saijoensis.
  
  
  CONCLUSIONS
  The polymorphic microsatellite markers developed in this study will be useful for investigating the levels of genetic diversity within remnant populations ofNuphartaxa and could provide a valuable tool for conservation genetics of these taxa.
authors.affiliation: Graduate School for International Development and Cooperation, Hiroshima University, Higashi-Hiroshima 739-8529, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Kondo
authors.affiliation: Graduate School for International Development and Cooperation, Hiroshima University, Higashi-Hiroshima 739-8529, Japan.
authors.forename: Sonoko
authors.initials: S
authors.lastname: Watanabe
authors.affiliation: Faculty of Education, Niigata University, Niigata 950-2181, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Shiga
authors.affiliation: Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Isagi
keywords: Nuphar japonica; Nymphaeaceae; aquatic plant species; cross-amplification; microsatellite