title: Determination of a broad spectrum of endocrine-disrupting pesticides in fish samples by UHPLC-MS/MS using the pass-through cleanup approach.
abstract: We describe a new methodology for the simultaneous determination of the endocrine-disrupting herbicides (acetochlor, alachlor, amitrole, and atrazine), fungicides (carbendazim, triadimefon, penconazole, and propiconazole), and insecticides (carbaryl and carbofuran) in fish samples followed by high-performance liquid chromatography with tandem mass spectrometry. Samples were extracted and purified using the pass-through cleanup approach. The recoveries of the pesticides were in the range 71.8-116.5%, with relative standard deviations lower than 15.28%. Limits of quantitation were in the range of 0.03-2.50 μg/kg. Validation results on linearity, accuracy, and precision, as well as on application to the analysis of the endocrine-disrupting pesticides in 20 fish samples, demonstrated the applicability to screen the presence of pesticides in fish.
authors.affiliation: Laboratory of Chemistry and Physics, Hangzhou Center for Disease Control and Prevention, Hangzhou, P. R.China.
authors.forename: Shaoying
authors.initials: S
authors.lastname: Liu
authors.affiliation: Laboratory of Chemistry and Physics, Hangzhou Center for Disease Control and Prevention, Hangzhou, P. R.China.
authors.forename: Xihui
authors.initials: X
authors.lastname: Huang
authors.affiliation: Laboratory of Chemistry and Physics, Hangzhou Center for Disease Control and Prevention, Hangzhou, P. R.China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Jin
authors.affiliation: Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou, P. R. China.
authors.forename: Guonian
authors.initials: G
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D052244:Endocrine Disruptors / Q000032:analysis*; D005399:Fishes; D005659:Fungicides, Industrial / Q000032:analysis; D007306:Insecticides / Q000032:analysis; D010575:Pesticides / Q000032:analysis*; D017747:Seafood / Q000032:analysis*; D053719:Tandem Mass Spectrometry
chemical_list: D052244:Endocrine Disruptors; D005659:Fungicides, Industrial; D007306:Insecticides; D010575:Pesticides
keywords: endocrine-disrupting pesticides; fish; mass spectrometry; pass-through cleanup approach