title: Purification, Characterization, Antioxidation, and Antiaging Properties of Exopolysaccharides and Endopolysaccharides of the Royal Sun Medicinal Mushroom, Agaricus brasiliensis (Agaricomycetes).
abstract: Exopolysaccharides (ExPSs) and endopolysaccharides (EnPSs) were isolated from mycelia and culture medium of Agaricus brasiliensis. Four polysaccharide fractions (ExPS-1-2, ExPS-2, EnPS-1, and EnPS-2) were puri¬fied from ExPSs and EnPSs using diethylaminoethyl anion exchange and a Sephadex G-100 gel filtration column, respectively, and their physicochemical properties were analyzed and evaluated to determine antioxidant activity. The results showed that rhamnose and mannose were the main monosaccharides of the polysaccharide fractions. ExPS-2 and EnPS-2 were acidic polysaccharide fractions and showed higher scavenging effects on 1,1-diphenyl-2-picryl- hydrazyl and hydroxyl radicals, and had higher reducing power in the in vitro antioxidant assays. In addition, the malondialdehyde contents, the total antioxidant capacity, and the alanine transaminase activity were also assessed to determine the antiaging effects; ExPSs showed more antiaging effects compared with EnPSs. The results suggest that polysaccharides extracted from A. brasiliensis have significant potential as natural antioxidants and antiaging agents.
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong, P. R. China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Gao
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong, P. R. China.
authors.forename: Shangshang
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong, P. R. China.
authors.forename: Shanglong
authors.initials: S
authors.lastname: Gao
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong, P. R. China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Ren
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong, P. R. China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Jing
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong, China.
authors.forename: Le
authors.initials: L
authors.lastname: Jia
mesh_terms: D000364:Agaricus / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000302:isolation & purification* / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003470:Culture Media / Q000737:chemistry; D017665:Hydroxyl Radical / Q000378:metabolism; D008297:Male; D051379:Mice; D025282:Mycelium / Q000737:chemistry; D010084:Oxidation-Reduction; D010851:Picrates / Q000378:metabolism; D011134:Polysaccharides / Q000008:administration & dosage / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D003470:Culture Media; D010851:Picrates; D011134:Polysaccharides; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl