title: Hydrogen-rich saline attenuates hippocampus endoplasmic reticulum stress after cardiac arrest in rats.
abstract: Hydrogen-rich saline can selectively scavenge reactive oxygen species (ROS) and protect brain against ischemia reperfusion (I/R) injury. Endoplasmic reticulum stress (ERS) has been implicated in the pathological process of cerebral ischemia. However, very little is known about the role of hydrogen-rich saline in mediating pathophysiological reactions to ERS after I/R injury caused by cardiac arrest.
  
  
  
  The rats were randomly divided into three groups, sham group (n=30), ischemia/reperfusion group (n=40) and hydrogen-rich saline group (n=40). The rats in experimental groups were subjected to 4min of cardiac arrest and followed by resuscitation. Then they were randomized to receive 5ml/kg of either hydrogen-rich saline or normal saline.
  
  
  
  Hydrogen-rich saline significantly improves survival rate and neurological function. The beneficial effects of hydrogen-rich saline were associated with decreased levels of oxidative products, as well as the increased levels of antioxidant enzymes. Furthermore, the protective effects of hydrogen-rich saline were accompanied by the increased activity of glucose-regulated protein 78 (GRP78), the decreased activity of cysteinyl aspartate specific proteinase-12 (caspase-12) and C/EBP homologous protein (CHOP).
  
  
  
  Hydrogen-rich saline attenuates brain I/R injury may through inhibiting hippocampus ERS after cardiac arrest in rats.
authors.affiliation: Department of Anesthesiology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Anesthesiology, Shanghai Meishan Hospital, Nanjing, China.
authors.forename: Qinfang
authors.initials: Q
authors.lastname: Gui
authors.affiliation: Department of Anesthesiology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Jin
authors.affiliation: Department of Burn and Plastic Surgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Yu
authors.affiliation: Department of Anesthesiology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Anesthesiology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China.
authors.forename: Liangbin
authors.initials: L
authors.lastname: Cao
authors.affiliation: Department of Anesthesiology, Shanghai Meishan Hospital, Nanjing, China. Electronic address: msyywq@163.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China. Electronic address: duanml1200@126.com.
authors.forename: Manlin
authors.initials: M
authors.lastname: Duan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000648:ultrastructure; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects*; D006323:Heart Arrest / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D006859:Hydrogen / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000494:pharmacology* / Q000627:therapeutic use; D015996:Survival Rate
chemical_list: D000975:Antioxidants; D012965:Sodium Chloride; D006859:Hydrogen
keywords: Apoptosis; CPR; Endoplasmic reticulum stress; Hydrogen; Oxidative stress