title: Repeatability and Reproducibility of Foveal Avascular Zone Area Measurements Using AngioPlex Spectral Domain Optical Coherence Tomography Angiography in Healthy Subjects.
abstract: OBJECTIVE
  The aim of this study was to evaluate the repeatability and reproducibility of foveal avascular zone (FAZ) area measurements using AngioPlex spectral domain optical coherence tomography (OCT) angiography in normal subjects.
  
  
  METHODS
  Twenty-two healthy subjects (25 eyes) underwent FAZ area measurements with AngioPlex OCT. Each volunteer was separately examined 3 consecutive times by the 2 experienced observers. The FAZ area was measured using ImageJ software. Intraobserver repeatability was evaluated by calculating the coefficient of variation (CoV) and intraclass correlation coefficient (ICC). Interobserver reproducibility was also assessed using the Bland-Altman test and concordance correlation coefficient (CCC).
  
  
  RESULTS
  The FAZ areas were measured as 0.373 ± 0.109 and 0.377 ± 0.112 mm2 by observers 1 and 2, respectively. The repeatability assessment of the FAZ area measurements yielded CoV values of 0.029 and 0.034 and ICC values of 0.997 and 0.996 by observers 1 and 2, respectively. The mean difference between the 2 observers was 0.004. CCC values ranged from 0.9705 to 0.9844.
  
  
  CONCLUSIONS
  The FAZ area measurements obtained using AngioPlex OCT showed a good repeatability and reproducibility in healthy subjects. Excellent reliability makes AngioPlex OCT a valid device for measuring the FAZ area.
authors.affiliation: Department of Ophthalmology, Shanghai General Hospital, Shanghai, China.
authors.forename: Jiaxian
authors.initials: J
authors.lastname: Guo
authors.forename: Xiangjun
authors.initials: X
authors.lastname: She
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Liu
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Sun
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D005451:Fluorescein Angiography / Q000379:methods*; D005654:Fundus Oculi; D064368:Healthy Volunteers; D006801:Humans; D008266:Macula Lutea / Q000000981:diagnostic imaging*; D008297:Male; D008875:Middle Aged; D012372:ROC Curve; D015203:Reproducibility of Results; D012171:Retinal Vessels / Q000000981:diagnostic imaging*; D041623:Tomography, Optical Coherence / Q000379:methods*