title: Preparation and biological evaluation of99mTc-HYNIC-(Ser)3-D4 peptide for targeting and imaging of non-small-cell lung cancer.
abstract: OBJECTIVE
  In this study, radiolabeled D4 peptide conjugate was studied as a radiotracer for imaging of non-small-cell lung cancer with overexpression of EGFR.
  
  
  METHODS
  HYNIC-(Ser)3-D4 peptide was labeled with99mTc using tricine as a co-ligand. Cellular specific binding and internalization as well as in vivo tumor targeting were assessed.
  
  
  RESULTS
  The in vitro experiments showed good cellular specific binding. Tumor uptake values as %ID/g were 7.55 and 6.82% at 1 and 4 h after injection, respectively. The presaturation of EGFR in xenografted nude mice reduced 36% tumor uptake of radioactivity at 1 h after injection that confirmed in vivo specificity.
  
  
  CONCLUSIONS
  Findings showed this radiolabeled peptide is a promising candidate for tumor targeting and molecular imaging of non-small-cell lung cancer.
authors.affiliation: Department of Radiopharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Mona
authors.initials: M
authors.lastname: Haddad Zahmatkesh
authors.affiliation: Department of Radiology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.|Cardiovascular Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Seyed Mohammad
authors.initials: SM
authors.lastname: Abedi
authors.affiliation: Department of Radiopharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Seyed Jalal
authors.initials: SJ
authors.lastname: Hosseinimehr
mesh_terms: D000818:Animals; D002289:Carcinoma, Non-Small-Cell Lung / Q000000981:diagnostic imaging*; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D064593:Heterografts; D006801:Humans; D008175:Lung Neoplasms / Q000000981:diagnostic imaging*; D008279:Magnetic Resonance Imaging; D008297:Male; D051379:Mice; D008819:Mice, Nude; D057054:Molecular Imaging / Q000379:methods*; D015609:Organotechnetium Compounds; D010455:Peptides; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals; D014018:Tissue Distribution
chemical_list: D015609:Organotechnetium Compounds; D010455:Peptides; D019275:Radiopharmaceuticals
keywords: 99mTc; EGFR; non-small-cell lung cancer; peptide; tumor targeting