title: Benzoic Acid Enhances Embryo Implantation through LIF-Dependent Expression of Integrin αVβ3 and αVβ5.
abstract: Embryo implantation is the crucial step for a successful pregnancy. Diverse factors, including adhesion molecules, growth factors, and cytokines are important for embryo implantation through improving endometrial receptivity. Benzoic acid (BA), a component of various plants, has been shown to have antifungal and antioxidant effects. However, the effect of BA on embryo implantation remains unknown. Here, we showed the contribution of BA for the enhancement of endometrial receptivity through the leukemia inhibitory factor (LIF)-dependent increase of integrin αV, β3, and β5 expression. Furthermore, in vivo study using a mifepristone-induced implantation failure model showed that BA definitely improves the numbers of implantation embryos. Taken together, we suggest that BA has a novel function for embryo implantation through the up-regulation of LIF-mediated integrins, and may be a candidate for therapeutic medicine to increase the pregnancy rate.
authors.affiliation: School of Korean Medicine and Healthy Aging Korean Medicine Research Center, Pusan National University, Yangsan 50612, Republic of Korea.
authors.forename: Hee-Jung
authors.initials: HJ
authors.lastname: Choi
authors.affiliation: School of Korean Medicine and Healthy Aging Korean Medicine Research Center, Pusan National University, Yangsan 50612, Republic of Korea.
authors.forename: Tae-Wook
authors.initials: TW
authors.lastname: Chung
authors.affiliation: School of Korean Medicine and Healthy Aging Korean Medicine Research Center, Pusan National University, Yangsan 50612, Republic of Korea.
authors.forename: Mi-Ju
authors.initials: MJ
authors.lastname: Park
authors.affiliation: Laboratory of Molecular Toxicology, School of Pharmacy, SungKyunKwan University, Suwon 16419, Republic of Korea.
authors.forename: Hyung Sik
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Korea Institute of Oriental Medicine, Daejeon 34054, Republic of Korea.
authors.forename: Sooseong
authors.initials: S
authors.lastname: You
authors.affiliation: Korea Institute of Oriental Medicine, Daejeon 34054, Republic of Korea.
authors.forename: Myeong Soo
authors.initials: MS
authors.lastname: Lee
authors.affiliation: School of Korean Medicine and Healthy Aging Korean Medicine Research Center, Pusan National University, Yangsan 50612, Republic of Korea.
authors.forename: Bo Sun
authors.initials: BS
authors.lastname: Joo
authors.affiliation: Department of Obstetrics and Gynecology, Pusan National University Hospital, Busan 49241, Republic of Korea.
authors.forename: Kyu Sup
authors.initials: KS
authors.lastname: Lee
authors.affiliation: Department of Clinical Pathology, DaeKyeung University, Gyeongsan 38547, Republic of Korea.
authors.forename: Keuk-Jun
authors.initials: KJ
authors.lastname: Kim
authors.affiliation: Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu 41061, Republic of Korea.
authors.forename: Gabbine
authors.initials: G
authors.lastname: Wee
authors.affiliation: Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu 41061, Republic of Korea.
authors.forename: Choong-Yong
authors.initials: CY
authors.lastname: Kim
authors.affiliation: Department of Biological Science, Sungkyunkwan University, Suwon 16419, Republic of Korea.
authors.forename: Cheorl-Ho
authors.initials: CH
authors.lastname: Kim
authors.affiliation: School of Korean Medicine and Healthy Aging Korean Medicine Research Center, Pusan National University, Yangsan 50612, Republic of Korea.
authors.forename: Ki-Tae
authors.initials: KT
authors.lastname: Ha
mesh_terms: D000818:Animals; D019817:Benzoic Acid / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D010064:Embryo Implantation / Q000187:drug effects*; D004717:Endometrium / Q000187:drug effects; D047628:Estrogen Receptor alpha; D005260:Female; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000378:metabolism*; D053605:Leukemia Inhibitory Factor / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011247:Pregnancy; D018873:Pregnancy Rate; D034741:RNA, Small Interfering; D019030:Receptors, Vitronectin / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D015815:Cell Adhesion Molecules; D016207:Cytokines; D047628:Estrogen Receptor alpha; D039302:Integrin alphaVbeta3; D053605:Leukemia Inhibitory Factor; D034741:RNA, Small Interfering; D019030:Receptors, Vitronectin; C064660:integrin alphaVbeta5; D019817:Benzoic Acid
keywords: Benzoic acid; adhesion; endometrial receptivity; implantation; integrin; leukemia inhibitory factor