title: Dual-channel probe of carbon dots cooperating with gold nanoclusters employed for assaying multiple targets.
abstract: Herein, carbon dots (CD@Papain) from papain have been originally synthesized in aqueous solution along with a quantum yield of 6.2%, and showed cyan fluorescence at 435nm. Meanwhile, papain-templated Au nanoclusters (AuNCs@Papain) were prepared with the same precursor. On the basis of the two types of nanomaterials, CD@Papain and AuNCs@Papain have been designed to assemble as one nanosensor (termed as CD-AuNCs@Papain) through a typical cross-linking reaction. Significantly, it not only emitted the dual-emission fluorescent signals with the same excitation, but also can assay multiple targets through exhibiting cyan, yellow, and red emission respectively. Thereby, H2O2, doxycycline and I-can be detected by CD-AuNCs@Papain. The mechanism of the fluorescence variations of CD-AuNCs@Papain: Au(0) of CD-AuNCs@Papain oxidized as Au(I) by H2O2, the formation of hydrogen bonds between the CD-AuNCs@Papain and I-, the inner filter effect (IFE) occurring caused by tetracyclines. Meanwhile, the detection limits of H2O2, doxycycline and I-were obtained as 0.3nM, 0.2nM, and 0.6nM at a signal-to-ratio of 3, respectively. These results suggested that the nanoprobe here has provided the possibility for rapidly assaying multiple targets with the acceptable selectivity.
authors.affiliation: College of Pharmaceutical Sciences, Engineer Research Center of Chongqing Pharmaceutical Process and Quality Control, Southwest University, Chongqing 400715, China.
authors.forename: Kuncheng
authors.initials: K
authors.lastname: Yang
authors.affiliation: College of Pharmaceutical Sciences, Engineer Research Center of Chongqing Pharmaceutical Process and Quality Control, Southwest University, Chongqing 400715, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Pharmaceutical Sciences, Engineer Research Center of Chongqing Pharmaceutical Process and Quality Control, Southwest University, Chongqing 400715, China.
authors.forename: Yingyi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Pharmaceutical Sciences, Engineer Research Center of Chongqing Pharmaceutical Process and Quality Control, Southwest University, Chongqing 400715, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Miao
authors.affiliation: College of Pharmaceutical Sciences, Engineer Research Center of Chongqing Pharmaceutical Process and Quality Control, Southwest University, Chongqing 400715, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Yang
mesh_terms: D000838:Anions / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000032:analysis; D002244:Carbon / Q000737:chemistry*; D004318:Doxycycline / Q000032:analysis; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis; D007455:Iodine / Q000032:analysis; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010206:Papain / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D000838:Anions; D000900:Anti-Bacterial Agents; D005456:Fluorescent Dyes; D002244:Carbon; D006046:Gold; D007455:Iodine; D006861:Hydrogen Peroxide; D010206:Papain; D004318:Doxycycline
keywords: Au nanoclusters; Carbon dots; Dual-channel; Multiple targets; Papain