title: Severe Aortic Valve Stenosis in Adults is Associated with Increased Levels of Circulating Intermediate Monocytes.
abstract: Individual monocyte subsets have been associated with atherosclerotic disease, but their distribution has not been evaluated in aortic valve stenosis (AS) so far. In the present study, we have asked whether levels of the circulating intermediate monocyte subset are increased in AS. Classical (CD14++CD16-), intermediate (CD14++CD16+), and non-classical (CD14+CD16++) CD86-positive monocytes and monocyte activation (intensity of CD11b expression) were determined by flow cytometry in peripheral blood of patients with severe AS (n = 100) and matched AS-free controls (n = 75). AS patients exhibited significantly higher levels of circulating intermediate monocytes, while levels of circulating classical and non-classical monocytes or monocyte activation did not differ compared to controls. The difference in levels of intermediate monocytes between groups was independent of age, gender, BMI, LDL-C, NT-proBNP, NYHA functional class, or creatinine levels. The present pilot study provides evidence of an association of severe AS with increased levels of circulating intermediate monocytes. Further studies need to clarify whether this finding is related to the inflammatory status and hemodynamic disturbances associated with severe AS.
authors.affiliation: Medizinische Klinik m.S. Kardiologie und Angiologie, Charité-Universitätsmedizin Berlin, Campus Mitte, Charitéplatz 1, 10117, Berlin, Germany. bernd.hewing@charite.de.|Berlin Institute of Health (BIH), Berlin, Germany. bernd.hewing@charite.de.|DZHK (German Center for Cardiovascular Research), partner site Berlin, Berlin, Germany. bernd.hewing@charite.de.
authors.forename: Bernd
authors.identifier: http://orcid.org/0000-0003-3734-2533
authors.initials: B
authors.lastname: Hewing
authors.affiliation: Medizinische Klinik m.S. Kardiologie und Angiologie, Charité-Universitätsmedizin Berlin, Campus Mitte, Charitéplatz 1, 10117, Berlin, Germany.|DZHK (German Center for Cardiovascular Research), partner site Berlin, Berlin, Germany.
authors.forename: Sebastian Chi-Diep
authors.initials: SC
authors.lastname: Au
authors.affiliation: Medizinische Klinik m.S. Kardiologie und Angiologie, Charité-Universitätsmedizin Berlin, Campus Mitte, Charitéplatz 1, 10117, Berlin, Germany.|DZHK (German Center for Cardiovascular Research), partner site Berlin, Berlin, Germany.
authors.forename: Antje
authors.initials: A
authors.lastname: Ludwig
authors.affiliation: Medizinische Klinik m.S. Kardiologie und Angiologie, Charité-Universitätsmedizin Berlin, Campus Mitte, Charitéplatz 1, 10117, Berlin, Germany.
authors.forename: Rena
authors.initials: R
authors.lastname: Ellerbroek
authors.affiliation: Medizinische Klinik m.S. Kardiologie und Angiologie, Charité-Universitätsmedizin Berlin, Campus Mitte, Charitéplatz 1, 10117, Berlin, Germany.
authors.forename: Phillip
authors.initials: P
authors.lastname: van Dijck
authors.affiliation: Berlin Institute of Health (BIH), Berlin, Germany.|Klinik für Allgemein-, Viszeral- und Gefäßchirurgie, Charité-Universitätsmedizin Berlin, Campus Benjamin Franklin, Berlin, Germany.
authors.forename: Lisa
authors.initials: L
authors.lastname: Hartmann
authors.affiliation: Klinik für Kardiovaskuläre Chirurgie, Charité-Universitätsmedizin Berlin, Campus Mitte, Berlin, Germany.
authors.forename: Herko
authors.initials: H
authors.lastname: Grubitzsch
authors.affiliation: Institut für Medizinische Immunologie und BCRT (Berlin-Brandenburg Center for Regenerative Therapies), Charité-Universitätsmedizin Berlin, Campus Virchow Klinikum, Berlin, Germany.
authors.forename: Carolin
authors.initials: C
authors.lastname: Giannini
authors.affiliation: Medizinische Klinik m.S. Kardiologie und Angiologie, Charité-Universitätsmedizin Berlin, Campus Mitte, Charitéplatz 1, 10117, Berlin, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Laule
authors.affiliation: Medizinische Klinik m.S. Kardiologie und Angiologie, Charité-Universitätsmedizin Berlin, Campus Mitte, Charitéplatz 1, 10117, Berlin, Germany.|DZHK (German Center for Cardiovascular Research), partner site Berlin, Berlin, Germany.
authors.forename: Verena
authors.initials: V
authors.lastname: Stangl
authors.affiliation: Medizinische Klinik m.S. Kardiologie und Angiologie, Charité-Universitätsmedizin Berlin, Campus Mitte, Charitéplatz 1, 10117, Berlin, Germany.
authors.forename: Gert
authors.initials: G
authors.lastname: Baumann
authors.affiliation: Medizinische Klinik m.S. Kardiologie und Angiologie, Charité-Universitätsmedizin Berlin, Campus Mitte, Charitéplatz 1, 10117, Berlin, Germany.|DZHK (German Center for Cardiovascular Research), partner site Berlin, Berlin, Germany.
authors.forename: Karl
authors.initials: K
authors.lastname: Stangl
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001024:Aortic Valve Stenosis / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D051940:B7-2 Antigen / Q000097:blood; D015415:Biomarkers / Q000097:blood; D039481:CD11b Antigen / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D058851:GPI-Linked Proteins / Q000097:blood; D006801:Humans; D007958:Leukocyte Count; D018950:Lipopolysaccharide Receptors / Q000097:blood; D008297:Male; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism; D010641:Phenotype; D010865:Pilot Projects; D011237:Predictive Value of Tests; D017452:Receptors, IgG / Q000097:blood; D012720:Severity of Illness Index
chemical_list: D051940:B7-2 Antigen; D015415:Biomarkers; D039481:CD11b Antigen; C497964:CD86 protein, human; C428200:FCGR3B protein, human; D058851:GPI-Linked Proteins; C509295:ITGAM protein, human; D018950:Lipopolysaccharide Receptors; D017452:Receptors, IgG
keywords: Aortic valve stenosis; Inflammation; Monocyte subsets