title: Efficacy of 50 Hz electromagnetic fields on human epidermal stem cell transplantation seeded in collagen sponge scaffolds for wound healing in a murine model.
abstract: To explore the possible efficacy of electromagnetic fields (EMF) for skin tissue engineering, effects of EMF exposure on epidermal stem cells (ESC) seeded in collagen sponge scaffolds for wound healing in a murine model were investigated. The wound models of a full-thickness defect established with 36 7 ∼ 8-week-old nude mice were randomly divided into three groups: a control group, an ESC-only group, and an ESC with EMF exposure group (frequency of 50 Hz, magnetic induction of 5 mT, 60 min per day for 20 days). ESC were separated from human foreskin and cultured in vitro, and then transplanted with collagen sponge scaffolds as a delivery vehicle to wounds of the ESC-only group, and ESC with EMF exposure group was exposed to EMF after ESC transplantation. Effects of EMF on morphological changes and expression of β1 integrin in regenerated skins were observed. Wound healing rates and healing times were collected to evaluate the efficacy of repairment. Results showed that human ESC were successfully transplanted to nude mice, which facilitated the formation of intact skin on nude mice. In contrast to other groups, the wound healing of ESC with EMF exposure group was the fastest (P < 0.05), the structure of regenerated skins was more mature, and it contained more continuity in the number of viable cell layers and rich hair follicles' structure. These results suggest that the use of 50 Hz EMF as a non-invasive treatment can accelerate wound healing of ESC transplantation, and restore structural integrity of regenerated skin. Bioelectromagnetics. 38:204-212,2017. © 2017 Wiley Periodicals, Inc.
authors.affiliation: Jinan University, Guangzhou, China.|Department of Rehabilitation Medicine, Institute of Geriatric Medicine, Guangdong Academy of Medical Sciences, Guangdong General Hospital, Guangzhou, China.
authors.forename: Wen-Fang
authors.initials: WF
authors.lastname: Bai
authors.affiliation: Department of Rehabilitation Medicine, Institute of Geriatric Medicine, Guangdong Academy of Medical Sciences, Guangdong General Hospital, Guangzhou, China.
authors.forename: Wei-Cheng
authors.initials: WC
authors.lastname: Xu
authors.affiliation: Department of Rehabilitation Medicine, Institute of Geriatric Medicine, Guangdong Academy of Medical Sciences, Guangdong General Hospital, Guangzhou, China.
authors.forename: Hong-Xiang
authors.initials: HX
authors.lastname: Zhu
authors.affiliation: School of Information, University of South Florida, Tampa, Florida.
authors.forename: Hong
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Rehabilitation Medicine, Institute of Geriatric Medicine, Guangdong Academy of Medical Sciences, Guangdong General Hospital, Guangzhou, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Rehabilitation Medicine, Institute of Geriatric Medicine, Guangdong Academy of Medical Sciences, Guangdong General Hospital, Guangzhou, China.
authors.forename: Ming-Sheng
authors.initials: MS
authors.lastname: Zhang
mesh_terms: D000818:Animals; D040761:Biomimetic Materials; D018929:Cell Culture Techniques; D024042:Collagen Type I; D004574:Electromagnetic Fields; D000078404:Epidermal Cells; D006801:Humans; D008297:Male; D008819:Mice, Nude; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D014945:Wound Healing / Q000502:physiology*
chemical_list: D024042:Collagen Type I
keywords: electromagnetic fields; epidermal stem cells; scaffold; transplantation; wound healing