title: The safe enterocin DD14 is a leaderless two-peptide bacteriocin with anti-Clostridium perfringens activity.
abstract: Enterococcus faecalis 14, a strain previously isolated from meconium, displayed activity against four Clostridium perfringens isolates when co-cultured on agar plates. The anti-Clostridium activity was ascribed to the production of enterocin DD14, which was subsequently purified. The minimum inhibitory concentration (MIC) of enterocin DD14 against one collection strain and one clinical C. perfringens strain was determined at 50 µg/mL. Furthermore, using the intestinal epithelial cell line IPEC-1, it was shown that E. faecalis 14 was not cytotoxic after 24 h of contact, and no cytotoxicity was observed when IPEC-1 cells were incubated with pure enterocin DD14 for 4 h. Enterocin DD14 was characterised using mass spectrometry and was shown to consist of two small proteins of 5200.74 Da and 5206.41 Da, respectively. The two peptides (DD14A and DD14B) have highly similar amino acid sequences and no signal peptide, which classifies enterocin DD14 as a class IIb leaderless two-peptide bacteriocin. The genes encoding DD14A and DD14B were sequenced and were shown to be 100% identical to other previously described enterocins MR10A and MR10B, in contrast to the producing strains, which are different. Consequently, the present in vitro study supports the potential of this E. faecalis 14 strain and/or its purified enterocin DD14 as putative anti-C. perfringens compounds in chickens.
authors.affiliation: Univ. Lille, INRA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394 - ICV - Institut Charles Viollette, F-59000 Lille, France.
authors.forename: Delphine L
authors.initials: DL
authors.lastname: Caly
authors.affiliation: Univ. Lille, INRA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394 - ICV - Institut Charles Viollette, F-59000 Lille, France.
authors.forename: Mickaël
authors.initials: M
authors.lastname: Chevalier
authors.affiliation: Univ. Artois. Univ. Lille, INRA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394 - ICV - Institut Charles Viollette, F-59000 Lille, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Flahaut
authors.affiliation: Univ. Lille, INRA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394 - ICV - Institut Charles Viollette, F-59000 Lille, France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Cudennec
authors.affiliation: Univ. Lille, INRA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394 - ICV - Institut Charles Viollette, F-59000 Lille, France.
authors.forename: Ahmed Khassaf
authors.initials: AK
authors.lastname: Al Atya
authors.affiliation: Univ. Lille, INRA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394 - ICV - Institut Charles Viollette, F-59000 Lille, France.
authors.forename: Gabrielle
authors.initials: G
authors.lastname: Chataigné
authors.affiliation: Phileo Lesaffre Animal Care, Société Industrielle Lesaffre, Marcq-en-Barœul, France.
authors.forename: Romain
authors.initials: R
authors.lastname: D'Inca
authors.affiliation: Phileo Lesaffre Animal Care, Société Industrielle Lesaffre, Marcq-en-Barœul, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Auclair
authors.affiliation: Univ. Lille, INRA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394 - ICV - Institut Charles Viollette, F-59000 Lille, France. Electronic address: djamel.drider@univ-lille1.fr.
authors.forename: Djamel
authors.initials: D
authors.lastname: Drider
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001952:Bridged-Ring Compounds / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival; D003016:Clostridium perfringens / Q000187:drug effects*; D013293:Enterococcus faecalis / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents; D001952:Bridged-Ring Compounds; D010455:Peptides; C012306:enterocin
keywords: Bacteriocin; Clostridium perfringens; Cytotoxicity; Enterococcus faecalis