title: Bacterial community structure and prevalence of Pusillimonas-like bacteria in aged landfill leachate.
abstract: Although several works have been performed from an engineering point of view, a limited number of studies have focused on microbial communities involved in the humification of aged landfill leachates. In this work, cultivation techniques, next-generation sequencing, and phospholipid fatty acid analysis were adopted to decrypt the diversity and the ecophysiological properties of the dominant microbiota in aged landfill leachate. Based on Illumina sequencing, Betaproteobacteria, Bacteroidetes, Actinobacteria, and Alphaproteobacteria dominated the aged landfill leachate. The main taxa identified at genus level were Pusillimonas-like bacteria and Leucobacter (41.46% of total reads), with all of them being also isolated through cultivation. The presence of Pusillimonas-like bacteria was also verified by the detection of cyclo17:0 and iso-19:0 fatty acids in aged landfill leachate microbiota. Despite that almost all bacterial isolates exhibited extracellular lipolytic ability, no particular specificity was observed in the type of substrate utilized. The prevalence of effective degraders, such as Pusillimonas-like bacteria, makes the aged landfill leachate an ideal source for isolation of novel microorganisms with potential in situ bioremediation uses.
authors.affiliation: Laboratory of Wastewater Management and Treatment Technologies, Department of Environmental Engineering, Democritus University of Thrace, Vas. Sofias 12, 67100, Xanthi, Greece.
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Remmas
authors.affiliation: Laboratory of Wastewater Management and Treatment Technologies, Department of Environmental Engineering, Democritus University of Thrace, Vas. Sofias 12, 67100, Xanthi, Greece.
authors.forename: Charikleia
authors.initials: C
authors.lastname: Roukouni
authors.affiliation: Laboratory of Wastewater Management and Treatment Technologies, Department of Environmental Engineering, Democritus University of Thrace, Vas. Sofias 12, 67100, Xanthi, Greece. sntougia@env.duth.gr.
authors.forename: Spyridon
authors.identifier: http://orcid.org/0000-0002-6389-692X
authors.initials: S
authors.lastname: Ntougias
mesh_terms: D039903:Actinobacteria / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D020561:Alphaproteobacteria / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D041963:Bacteroidetes / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D020562:Betaproteobacteria / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D001673:Biodegradation, Environmental; D057919:Biological Oxygen Demand Analysis; D064307:Microbiota; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D064091:Waste Disposal Facilities; D062065:Wastewater / Q000032:analysis / Q000382:microbiology*; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry
chemical_list: D010743:Phospholipids; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Ecophysiological profile; Fatty acid biomarker; Humification; Illumina sequencing; Leucobacter; Pusillimonas; Specialized degrading consortia