title: Aqueous Humor Antimicrobial Activity: In Vitro Analysis after Topical 0.5% Chloramphenicol Application.
abstract: To assess aqueous humor antimicrobial activity in vitro after topical 0.5% chloramphenicol application.
  
  
  
  This investigation included 63 eyes from 65 cataract surgery patients. The study group of 48 eyes received preoperatively four topical applications of 0.5% chloramphenicol. The control group of 15 eyes was given no topical applications. Aqueous humor samples were collected for in vitro antimicrobial analysis using Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, and Pasteurella multocida organisms by means of disk diffusion test.
  
  
  
  No inhibition halo was observed around all aqueous humor samples from all chloramphenicol-treated patients, irrespective of the sample quantity added to the paper disks, with no significant difference from aqueous humor from untreated control patients.
  
  
  
  Aqueous humor displayed no bactericidal effect against any of the microorganisms evaluated after topical 0.5% chloramphenicol application.
authors.affiliation: a Department of Surgery and Biomedical Science , University of Perugia, Ospedale S. Maria della Misericordia , Perugia , Italy.
authors.forename: Carlo
authors.identifier: 0000-0002-3812-9219
authors.initials: C
authors.lastname: Cagini
authors.affiliation: a Department of Surgery and Biomedical Science , University of Perugia, Ospedale S. Maria della Misericordia , Perugia , Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Dragoni
authors.affiliation: a Department of Surgery and Biomedical Science , University of Perugia, Ospedale S. Maria della Misericordia , Perugia , Italy.
authors.forename: Giampaolo
authors.initials: G
authors.lastname: Orsolini
authors.affiliation: a Department of Surgery and Biomedical Science , University of Perugia, Ospedale S. Maria della Misericordia , Perugia , Italy.
authors.forename: Tito
authors.initials: T
authors.lastname: Fiore
authors.affiliation: a Department of Surgery and Biomedical Science , University of Perugia, Ospedale S. Maria della Misericordia , Perugia , Italy.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Beccasio
authors.affiliation: b Department of Biotechnology and Medical-Surgical Science , "Sapienza" University of Rome , Latina , Italy.
authors.forename: Leopoldo
authors.initials: L
authors.lastname: Spadea
authors.affiliation: c Department of Experimental Medicine , Microbiology Unit, University of Perugia, Ospedale S. Maria della Misericordia , Perugia , Italy.
authors.forename: Amedeo
authors.initials: A
authors.lastname: Moretti
authors.affiliation: c Department of Experimental Medicine , Microbiology Unit, University of Perugia, Ospedale S. Maria della Misericordia , Perugia , Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Mencacci
mesh_terms: D000287:Administration, Topical; D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D001082:Aqueous Humor / Q000737:chemistry / Q000187:drug effects / Q000382:microbiology*; D001419:Bacteria / Q000187:drug effects / Q000302:isolation & purification*; D002701:Chloramphenicol / Q000008:administration & dosage*; D015818:Eye Infections, Bacterial / Q000188:drug therapy / Q000378:metabolism / Q000382:microbiology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D009883:Ophthalmic Solutions
chemical_list: D000900:Anti-Bacterial Agents; D009883:Ophthalmic Solutions; D002701:Chloramphenicol
keywords: Anterior chamber; antimicrobial activity; aqueous humor; chloramphenicol; penetration