title: Toxicogenetic study of Persea americana fruit pulp oil and its effect on genomic instability.
abstract: Persea americana Mill., commonly known as avocado, is a tree native to Central America that is widely used as a food source and for the treatment of diseases. This plant has various biological properties such as analgesic, anti-inflammatory and total cholesterol-lowering activity. In view of its pharmacological potential, we conducted a toxicogenetic study of the fruit pulp oil of P. americana (PAO) and investigated its influence on genotoxicity induced by methyl methanesulfonate (MMS) and doxorubicin. V79 cells and Swiss mice were used for the assays. The results showed no genotoxic effects of PAO in the in vitro or in vivo test systems. However, the highest PAO dose tested led to an increase in the levels of aspartate aminotransferase, indicating hepatic/tissue damage. This effect may be related to high concentrations of palmitic acid, the main component of PAO. Furthermore, PAO was effective in reducing the chromosome damage induced by MMS and doxorubicin. These results contribute to the safety assessment of PAO as a medicinal plant for human use.
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600, Franca, São Paulo, Brazil.
authors.forename: Heloiza Diniz
authors.initials: HD
authors.lastname: Nicolella
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600, Franca, São Paulo, Brazil.
authors.forename: Francisco Rinaldi
authors.initials: FR
authors.lastname: Neto
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600, Franca, São Paulo, Brazil.
authors.forename: Mariana Beltrame
authors.initials: MB
authors.lastname: Corrêa
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600, Franca, São Paulo, Brazil.
authors.forename: Danillo Henrique
authors.initials: DH
authors.lastname: Lopes
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600, Franca, São Paulo, Brazil.
authors.forename: Edilaura Nunes
authors.initials: EN
authors.lastname: Rondon
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600, Franca, São Paulo, Brazil.
authors.forename: Luiz Felipe Ribeiro
authors.initials: LFR
authors.lastname: Dos Santos
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600, Franca, São Paulo, Brazil.
authors.forename: Pollyanna Francielli
authors.initials: PF
authors.lastname: de Oliveira
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600, Franca, São Paulo, Brazil.
authors.forename: Jaqueline Lopes
authors.initials: JL
authors.lastname: Damasceno
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600, Franca, São Paulo, Brazil.
authors.forename: Nathália Oliveira
authors.initials: NO
authors.lastname: Acésio
authors.affiliation: Departamento de Física e Química da Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Avenida do Café, s/n - Vila Monte Alegre, 14040-020, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Izabel Cristina Casanova
authors.initials: ICC
authors.lastname: Turatti
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600, Franca, São Paulo, Brazil.
authors.forename: Marcos Gomide
authors.initials: MG
authors.lastname: Tozatti
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600, Franca, São Paulo, Brazil.
authors.forename: Wilson Roberto
authors.initials: WR
authors.lastname: Cunha
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600, Franca, São Paulo, Brazil.
authors.forename: Ricardo Andrade
authors.initials: RA
authors.lastname: Furtado
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600, Franca, São Paulo, Brazil. Electronic address: denisecrispim2001@yahoo.com.
authors.forename: Denise Crispim
authors.initials: DC
authors.lastname: Tavares
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001681:Biological Assay / Q000379:methods; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000187:drug effects*; D003412:Cricetulus; D004249:DNA Damage / Q000187:drug effects*; D004317:Doxorubicin / Q000633:toxicity; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005638:Fruit / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D042822:Genomic Instability / Q000187:drug effects*; D006801:Humans; D008168:Lung / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008741:Methyl Methanesulfonate / Q000633:toxicity; D051379:Mice; D015162:Micronucleus Tests; D027421:Persea / Q000737:chemistry*; D010936:Plant Extracts / Q000633:toxicity*; D043922:Toxicogenetics / Q000379:methods*
chemical_list: D000903:Antibiotics, Antineoplastic; D010936:Plant Extracts; D004317:Doxorubicin; D008741:Methyl Methanesulfonate; D001219:Aspartate Aminotransferases
keywords: Antioxidant; Chemoprevention; Cytotoxicity; Genotoxicity; Persea americana