title: Glycoprotein Enrichment Analytical Techniques: Advantages and Disadvantages.
abstract: Protein glycosylation is one of the most important posttranslational modifications. Numerous biological functions are related to protein glycosylation. However, analytical challenges remain in the glycoprotein analysis. To overcome the challenges associated with glycoprotein analysis, many analytical techniques were developed in recent years. Enrichment methods were used to improve the sensitivity of detection, while HPLC and mass spectrometry methods were developed to facilitate the separation of glycopeptides/proteins and enhance detection, respectively. Fragmentation techniques applied in modern mass spectrometers allow the structural interpretation of glycopeptides/proteins, while automated software tools started replacing manual processing to improve the reliability and throughput of the analysis. In this chapter, the current methodologies of glycoprotein analysis were discussed. Multiple analytical techniques are compared, and advantages and disadvantages of each technique are highlighted.
authors.affiliation: Texas Tech University, Lubbock, TX, United States.
authors.forename: R
authors.initials: R
authors.lastname: Zhu
authors.affiliation: Texas Tech University, Lubbock, TX, United States.
authors.forename: L
authors.initials: L
authors.lastname: Zacharias
authors.affiliation: Texas Tech University, Lubbock, TX, United States.
authors.forename: K M
authors.initials: KM
authors.lastname: Wooding
authors.affiliation: Texas Tech University, Lubbock, TX, United States.
authors.forename: W
authors.initials: W
authors.lastname: Peng
authors.affiliation: Texas Tech University, Lubbock, TX, United States. Electronic address: yehia.mechref@ttu.edu.
authors.forename: Y
authors.initials: Y
authors.lastname: Mechref
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D006020:Glycopeptides / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D006020:Glycopeptides; D006023:Glycoproteins
keywords: Enrichment; Glycoprotein; Glycoproteomics; LC-MS/MS; Proteomics