title: Heat stress induces ferroptosis-like cell death in plants.
abstract: In plants, regulated cell death (RCD) plays critical roles during development and is essential for plant-specific responses to abiotic and biotic stresses. Ferroptosis is an iron-dependent, oxidative, nonapoptotic form of cell death recently described in animal cells. In animal cells, this process can be triggered by depletion of glutathione (GSH) and accumulation of lipid reactive oxygen species (ROS). We investigated whether a similar process could be relevant to cell death in plants. Remarkably, heat shock (HS)-induced RCD, but not reproductive or vascular development, was found to involve a ferroptosis-like cell death process. In root cells, HS triggered an iron-dependent cell death pathway that was characterized by depletion of GSH and ascorbic acid and accumulation of cytosolic and lipid ROS. These results suggest a physiological role for this lethal pathway in response to heat stress in Arabidopsis thaliana The similarity of ferroptosis in animal cells and ferroptosis-like death in plants suggests that oxidative, iron-dependent cell death programs may be evolutionarily ancient.
authors.affiliation: Instituto de Investigaciones Biológicas, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Mar del Plata, 7600 Mar del Plata, Argentina.
authors.forename: Ayelén Mariana
authors.identifier: http://orcid.org/0000-0003-3763-4501
authors.initials: AM
authors.lastname: Distéfano
authors.affiliation: Instituto de Investigaciones Biológicas, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Mar del Plata, 7600 Mar del Plata, Argentina.
authors.forename: María Victoria
authors.identifier: http://orcid.org/0000-0001-6015-7059
authors.initials: MV
authors.lastname: Martin
authors.affiliation: Instituto de Investigaciones Biológicas, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Mar del Plata, 7600 Mar del Plata, Argentina.
authors.forename: Juan Pablo
authors.identifier: http://orcid.org/0000-0002-8280-4824
authors.initials: JP
authors.lastname: Córdoba
authors.affiliation: Instituto de Investigaciones Biológicas, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Mar del Plata, 7600 Mar del Plata, Argentina.
authors.forename: Andrés Martín
authors.identifier: http://orcid.org/0000-0001-6883-2469
authors.initials: AM
authors.lastname: Bellido
authors.affiliation: Instituto de Investigaciones Biológicas, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Mar del Plata, 7600 Mar del Plata, Argentina.
authors.forename: Sebastián
authors.initials: S
authors.lastname: D'Ippólito
authors.affiliation: Instituto de Investigaciones Biológicas, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Mar del Plata, 7600 Mar del Plata, Argentina.
authors.forename: Silvana Lorena
authors.initials: SL
authors.lastname: Colman
authors.affiliation: Instituto de Investigaciones Biológicas, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Mar del Plata, 7600 Mar del Plata, Argentina.
authors.forename: Débora
authors.initials: D
authors.lastname: Soto
authors.affiliation: Instituto de Investigaciones Biológicas, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Mar del Plata, 7600 Mar del Plata, Argentina.
authors.forename: Juan Alfredo
authors.identifier: http://orcid.org/0000-0001-7712-2717
authors.initials: JA
authors.lastname: Roldán
authors.affiliation: Instituto de Fisiología Vegetal, Facultad de Ciencias Naturales, Universidad Nacional de La Plata Centro Científico Technológico La Plata CONICET, 1900 La Plata, Argentina.
authors.forename: Carlos Guillermo
authors.initials: CG
authors.lastname: Bartoli
authors.affiliation: Instituto de Investigaciones Biológicas, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Mar del Plata, 7600 Mar del Plata, Argentina.
authors.forename: Eduardo Julián
authors.identifier: http://orcid.org/0000-0001-5767-141X
authors.initials: EJ
authors.lastname: Zabaleta
authors.affiliation: Instituto de Investigaciones Biológicas, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Mar del Plata, 7600 Mar del Plata, Argentina.
authors.forename: Diego Fernando
authors.initials: DF
authors.lastname: Fiol
authors.affiliation: Department of Biological Sciences, Columbia University, New York, NY 10027.|Department of Chemistry, Columbia University, New York, NY 10027.
authors.forename: Brent R
authors.identifier: http://orcid.org/0000-0002-3532-3868
authors.initials: BR
authors.lastname: Stockwell
authors.affiliation: Department of Biology, Stanford University, Stanford, CA 94305 gpagnussat@mdp.edu.ar sjdixon@stanford.edu.
authors.forename: Scott J
authors.identifier: http://orcid.org/0000-0001-6230-8199
authors.initials: SJ
authors.lastname: Dixon
authors.affiliation: Instituto de Investigaciones Biológicas, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Mar del Plata, 7600 Mar del Plata, Argentina gpagnussat@mdp.edu.ar sjdixon@stanford.edu.
authors.forename: Gabriela Carolina
authors.identifier: http://orcid.org/0000-0002-6836-3495
authors.initials: GC
authors.lastname: Pagnussat
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism*; D001205:Ascorbic Acid / Q000378:metabolism; D016923:Cell Death; D019143:Evolution, Molecular; D005978:Glutathione / Q000378:metabolism; D018869:Heat-Shock Response / Q000187:drug effects; D006358:Hot Temperature; D007501:Iron / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000494:pharmacology; D015227:Lipid Peroxidation; D008856:Microscopy, Fluorescence; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors
chemical_list: D000975:Antioxidants; D007502:Iron Chelating Agents; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D007501:Iron; D005978:Glutathione; D001205:Ascorbic Acid