title: α3 Chains of type V collagen regulate breast tumour growth via glypican-1.
abstract: Pericellular α3(V) collagen can affect the functioning of cells, such as adipocytes and pancreatic β cells. Here we show that α3(V) chains are an abundant product of normal mammary gland basal cells, and that α3(V) ablation in a mouse mammary tumour model inhibits mammary tumour progression by reducing the proliferative potential of tumour cells. These effects are shown to be primarily cell autonomous, from loss of α3(V) chains normally produced by tumour cells, in which they affect growth by enhancing the ability of cell surface proteoglycan glypican-1 to act as a co-receptor for FGF2. Thus, a mechanism is presented for microenvironmental influence on tumour growth. α3(V) chains are produced in both basal-like and luminal human breast tumours, and its expression levels are tightly coupled with those of glypican-1 across breast cancer types. Evidence indicates α3(V) chains as potential targets for inhibiting tumour growth and as markers of oncogenic transformation.
authors.affiliation: Department of Cell and Regenerative Biology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin 53705, USA.
authors.forename: Guorui
authors.initials: G
authors.lastname: Huang
authors.affiliation: Department of Cell and Regenerative Biology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin 53705, USA.
authors.forename: Gaoxiang
authors.initials: G
authors.lastname: Ge
authors.affiliation: Centre of Excellence in Cell-Extracellular Matrix Research and Biocenter Oulu, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Aapistie 5, Oulu 92100, Finland.
authors.forename: Valerio
authors.initials: V
authors.lastname: Izzi
authors.affiliation: Department of Cell and Regenerative Biology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin 53705, USA.
authors.forename: Daniel S
authors.initials: DS
authors.lastname: Greenspan
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism; D003094:Collagen / Q000235:genetics / Q000378:metabolism*; D024062:Collagen Type V / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D053673:Glypicans / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D015674:Mammary Neoplasms, Animal; D051379:Mice; D018345:Mice, Knockout
chemical_list: C578895:Col5a3 protein, mouse; D024062:Collagen Type V; D053673:Glypicans; D003094:Collagen