title: Comparative Phosphoproteomics Reveals an Important Role of MKK2 in Banana (Musa spp.) Cold Signal Network.
abstract: Low temperature is one of the key environmental stresses, which greatly affects global banana production. However, little is known about the global phosphoproteomes in Musa spp. and their regulatory roles in response to cold stress. In this study, we conducted a comparative phosphoproteomic profiling of cold-sensitive Cavendish Banana and relatively cold tolerant Dajiao under cold stress. Phosphopeptide abundances of five phosphoproteins involved in MKK2 interaction network, including MKK2, HY5, CaSR, STN7 and kinesin-like protein, show a remarkable difference between Cavendish Banana and Dajiao in response to cold stress. Western blotting of MKK2 protein and its T31 phosphorylated peptide verified the phosphoproteomic results of increased T31 phosphopeptide abundance with decreased MKK2 abundance in Daojiao for a time course of cold stress. Meanwhile increased expression of MKK2 with no detectable T31 phosphorylation was found in Cavendish Banana. These results suggest that the MKK2 pathway in Dajiao, along with other cold-specific phosphoproteins, appears to be associated with the molecular mechanisms of high tolerance to cold stress in Dajiao. The results also provide new evidence that the signaling pathway of cellular MKK2 phosphorylation plays an important role in abiotic stress tolerance that likely serves as a universal plant cold tolerance mechanism.
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro- bioresources, South China Agricultural University, Guangzhou, 510640, China.|Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.|Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, 510640, China.|The Guangzhou Research Branch of the National Banana Improvement Center, Guangzhou, 510640, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.affiliation: Institute of Biotechnology, Cornell University, Ithaca, NY, USA.
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.|Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, 510640, China.|The Guangzhou Research Branch of the National Banana Improvement Center, Guangzhou, 510640, China.|Key Laboratory of Horticultural Plant Biology of the Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China.
authors.forename: Wei-Di
authors.initials: WD
authors.lastname: He
authors.affiliation: Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.|Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, 510640, China.|The Guangzhou Research Branch of the National Banana Improvement Center, Guangzhou, 510640, China.
authors.forename: Xiu-Hong
authors.initials: XH
authors.lastname: Shao
authors.affiliation: Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.|Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, 510640, China.|The Guangzhou Research Branch of the National Banana Improvement Center, Guangzhou, 510640, China.
authors.forename: Chun-Yu
authors.initials: CY
authors.lastname: Li
authors.affiliation: Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.|Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, 510640, China.|The Guangzhou Research Branch of the National Banana Improvement Center, Guangzhou, 510640, China.
authors.forename: Yue-Rong
authors.initials: YR
authors.lastname: Wei
authors.affiliation: Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.|Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, 510640, China.|The Guangzhou Research Branch of the National Banana Improvement Center, Guangzhou, 510640, China.
authors.forename: Gui-Ming
authors.initials: GM
authors.lastname: Deng
authors.affiliation: Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.|Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, 510640, China.|The Guangzhou Research Branch of the National Banana Improvement Center, Guangzhou, 510640, China.
authors.forename: Rui-Bin
authors.initials: RB
authors.lastname: Kuang
authors.affiliation: Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.|Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, 510640, China.|The Guangzhou Research Branch of the National Banana Improvement Center, Guangzhou, 510640, China.
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Hu
authors.affiliation: Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.|Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, 510640, China.|The Guangzhou Research Branch of the National Banana Improvement Center, Guangzhou, 510640, China.
authors.forename: Gan-Jun
authors.initials: GJ
authors.lastname: Yi
authors.affiliation: Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.|Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, 510640, China.|The Guangzhou Research Branch of the National Banana Improvement Center, Guangzhou, 510640, China.
authors.forename: Qiao-Song
authors.initials: QS
authors.lastname: Yang
mesh_terms: D000220:Adaptation, Biological; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D003080:Cold Temperature; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism*; D028521:Musa / Q000378:metabolism*; D010641:Phenotype; D010748:Phosphopeptides / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D010940:Plant Proteins / Q000378:metabolism*; D025941:Protein Interaction Mapping; D060066:Protein Interaction Maps; D020543:Proteome; D040901:Proteomics / Q000379:methods; D013312:Stress, Physiological
chemical_list: D010748:Phosphopeptides; D010750:Phosphoproteins; D010940:Plant Proteins; D020543:Proteome; D020929:Mitogen-Activated Protein Kinase Kinases