title: Stromal interaction molecule 1 regulates growth, cell cycle, and apoptosis of human tongue squamous carcinoma cells.
abstract: Oral tongue squamous cell carcinoma (OTSCC) is the most common type of oral carcinomas. However, the molecular mechanism by which OTSCC developed is not fully identified. Stromal interaction molecule 1 (STIM1) is a transmembrane protein, mainly located in the endoplasmic reticulum (ER). STIM1 is involved in several types of cancers. Here, we report that STIM1 contributes to the development of human OTSCC. We knocked down STIM1 in OTSCC cell line Tca-8113 with lentivirus-mediated shRNA and found that STIM1 knockdown repressed the proliferation of Tca-8113 cells. In addition, we also showed that STIM1 deficiency reduced colony number of Tca-8113 cells. Knockdown of STIM1 repressed cells to enter M phase of cell cycle and induced cellular apoptosis. Furthermore, we performed microarray and bioinformatics analysis and found that STIM1 was associated with p53 and MAPK pathways, which may contribute to the effects of STIM1 on cell growth, cell cycle, and apoptosis. Finally, we confirmed that STIM1 controlled the expression of MDM2, cyclin-dependent kinase 4 (CDK4), and growth arrest and DNA damage inducible α (GADD45A) in OTSCC cells. In conclusion, we provide evidence that STIM1 contributes to the development of OTSCC partially through regulating p53 and MAPK pathways to promote cell cycle and survival.
authors.affiliation: Section of Inner Mongolia Medical University Affiliated Hospital Otorhinolaryngology, Inner Mongolia Medical University Affiliated Hospital, Hohhot, Inner Mongolia 010050, People's Republic of China nmcxb@sina.com.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Cui
authors.affiliation: Section of Inner Mongolia Medical University Affiliated Hospital Otorhinolaryngology, Inner Mongolia Medical University Affiliated Hospital, Hohhot, Inner Mongolia 010050, People's Republic of China.
authors.forename: Laixiao
authors.initials: L
authors.lastname: Song
authors.affiliation: Section of Inner Mongolia Medical University Affiliated Hospital Otorhinolaryngology, Inner Mongolia Medical University Affiliated Hospital, Hohhot, Inner Mongolia 010050, People's Republic of China.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Section of Inner Mongolia Medical University Affiliated Hospital Otorhinolaryngology, Inner Mongolia Medical University Affiliated Hospital, Hohhot, Inner Mongolia 010050, People's Republic of China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Section of Inner Mongolia Medical University Affiliated Hospital Otorhinolaryngology, Inner Mongolia Medical University Affiliated Hospital, Hohhot, Inner Mongolia 010050, People's Republic of China.
authors.forename: Boqian
authors.initials: B
authors.lastname: Wang
authors.affiliation: Section of Inner Mongolia Medical University Affiliated Hospital Otorhinolaryngology, Inner Mongolia Medical University Affiliated Hospital, Hohhot, Inner Mongolia 010050, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D018797:Cell Cycle Proteins / Q000378:metabolism; D002455:Cell Division / Q000502:physiology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology*; D051358:Cyclin-Dependent Kinase 4 / Q000378:metabolism; D006801:Humans; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism; D051736:Proto-Oncogene Proteins c-mdm2 / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D000071737:Stromal Interaction Molecule 1 / Q000378:metabolism*; D014062:Tongue Neoplasms / Q000378:metabolism* / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D000097642:GADD45 Proteins
chemical_list: D018797:Cell Cycle Proteins; D051358:Cyclin-Dependent Kinase 4; D020928:Mitogen-Activated Protein Kinases; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D051736:Proto-Oncogene Proteins c-mdm2; D034741:RNA, Small Interfering; D000071737:Stromal Interaction Molecule 1; D016159:Tumor Suppressor Protein p53; C439070:GADD45A protein, human; D000097642:GADD45 Proteins; C103530:STIM1 protein, human; C495900:CDK4 protein, human
keywords: STIM1; Tca-8113 cells; apoptosis; cell cycle; tongue squamous carcinoma