title: Human NK Cell Subsets in Pregnancy and Disease: Toward a New Biological Complexity.
abstract: In humans, NK cells are mainly identified by the surface expression levels of CD56 and CD16, which differentiate between five functionally different NK cell subsets. However, nowadays NK cells are considered as a more heterogeneous population formed by various subsets differing in function, surface phenotype, and anatomic localization. In human CMV- and hantaviruses-infected subjects, an increased frequency of a NKG2A-CD57+NKG2C+NK cell subset has been observed, while the phenotype of the NK cell subpopulation associated with cancer may vary according to the specific kind of tumor and its anatomical location. The healthy human lymph nodes contain mainly the CD56brightNK cell subset while in melanoma metastatic lymph nodes the CD56dimCD57+KIR+CCR7+NK cell subpopulation prevails. The five NK cell subpopulations are found in breast cancer patients, where they differ for expression pattern of chemokine receptors, maturation stage, functional capabilities. In pregnancy, uterine NK cells show a prevalence of the CD56brightCD16-NK cell compartment, whose activity is influenced by KIRs repertoire. This NK cell subset's super specialization could be explained by (i) the expansion of single mature CD56dimclones, (ii) the recruitment and maturation of CD56brightNK cells through specific stimuli, and (iii) thein situdevelopment of tumor-resident NK cells from tissue-resident CD56brightNK cells independently of the circulating NK cell compartment. This new and unexpected biological feature of the NK cell compartment could be an important source of new biomarkers to improve patients' diagnosis.
authors.affiliation: Department of Experimental and Clinical Medicine, University Magna Graecia of Catanzaro , Catanzaro , Italy.
authors.forename: Costanza Maria
authors.initials: CM
authors.lastname: Cristiani
authors.affiliation: Department of Experimental and Clinical Medicine, University Magna Graecia of Catanzaro , Catanzaro , Italy.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Palella
authors.affiliation: Department of Microbiology, Cell and Tumor Biology, Karolinska Institutet , Stockholm , Sweden.
authors.forename: Rosa
authors.initials: R
authors.lastname: Sottile
authors.affiliation: Department of Experimental and Clinical Medicine, University Magna Graecia of Catanzaro , Catanzaro , Italy.
authors.forename: Rossana
authors.initials: R
authors.lastname: Tallerico
authors.affiliation: Department of Experimental and Clinical Medicine, University Magna Graecia of Catanzaro , Catanzaro , Italy.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Garofalo
authors.affiliation: Department of Experimental and Clinical Medicine, University Magna Graecia of Catanzaro, Catanzaro, Italy; Department of Microbiology, Cell and Tumor Biology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Ennio
authors.initials: E
authors.lastname: Carbone
keywords: NK cells; anatomical distribution; chemokines; diagnosis; melanoma