title: Exercise induced upregulation of glutamate-cysteine ligase catalytic subunit and glutamate-cysteine ligase modifier subunit gene expression in Thoroughbred horses.
abstract: OBJECTIVE
  This study was performed to reveal the molecular structure and expression patterns of horse glutamate-cysteine ligase catalytic subunit (GCLC) and glutamate-cysteine ligase modifier subunit (GCLM) genes whose products form glutamate cysteine ligase, which were identified as differentially expressed genes in the previous study.
  
  
  METHODS
  We performed bioinformatics analyses, and gene expression assay with quantitative polymerase chain reaction (qPCR) for horseGCLCandGCLMgenes in muscle and blood leukocytes of Thoroughbred horses.
  
  
  RESULTS
  Expression ofGCLCshowed the same pattern in both blood and muscle tissues after exercise. Expression ofGCLCincreased in the muscle and blood of Thoroughbreds, suggesting a tissue-specific regulatory mechanism for the expression ofGCLC. In addition, expression of theGCLMgene increased after exercise in both the blood and muscle of Thoroughbreds.
  
  
  CONCLUSIONS
  We established the expression patterns ofGCLCandGCLMin the skeletal muscle and blood of Thoroughbred horses in response to exercise. Further study is now warranted to uncover the functional importance of these genes in exercise and recovery in racehorses.
authors.affiliation: Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University, Miryang 50463, Korea.
authors.forename: Jeong-Woong
authors.initials: JW
authors.lastname: Park
authors.affiliation: Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University, Miryang 50463, Korea.
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Choi
authors.affiliation: Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University, Miryang 50463, Korea.
authors.forename: Seul A
authors.initials: SA
authors.lastname: Hong
authors.affiliation: National Institute of Animal Science, Rural Development Administration, Jeju 63242, Korea.
authors.forename: Nam Young
authors.initials: NY
authors.lastname: Kim
authors.affiliation: Department of Animal Biotehnology, Faculty of Biotechnology, Jeju National University, Jeju 63243, Korea.
authors.forename: Kyoung-Tag
authors.initials: KT
authors.lastname: Do
authors.affiliation: Department of Animal Biotechnology, Chonbuk National, University, Jeonju 54896, Korea.
authors.forename: Ki-Duk
authors.initials: KD
authors.lastname: Song
authors.affiliation: Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University, Miryang 50463, Korea.
authors.forename: Byung-Wook
authors.initials: BW
authors.lastname: Cho
keywords: Exercise; Glutamate-cysteine Ligase Catalytic Subunit (GCLC); Glutamate-cysteine Ligase Modifier Subunit (GCLM); Horse; Quantitative Polymerase Chain Reaction