title: A multi-walled carbon nanotubes based molecularly imprinted polymers electrochemical sensor for the sensitive determination of HIV-p24.
abstract: To develop a rapid, simple and sensitive method for the determination of human immunodeficiency virus p24 (HIV-p24), a novel molecularly imprinted polymers (MIPs) electrochemical sensor was constructed on the surface of a multi-walled carbon nanotubes (MWCNTs) modified glassy carbon electrode (GCE) by surface polymerization using acrylamide (AAM) as functional monomer, N,N'-methylenebisacrylamide (MBA) as cross-linking agent and ammonium persulphate (APS) as initiator. Each modification step was carefully examined by cyclic voltammetry (CV), differential pulse voltammetry (DPV) and scanning electron microscope (SEM). The proposed MIPs electrochemical biosensor exhibited specific recognition to HIV-p24 and displayed a broad linear detection range from 1.0×10-4to 2ngcm-3with a low detection limit of 0.083pgcm-3(S/N=3). This performance is superior to most HIV-p24 sensors based on other methods. Meanwhile, this sensor possessed of good selectivity, repeatability, reproducibility, stability and was successfully applied for the determination of HIV-p24 in real human serum samples, giving satisfactory results. The accuracy and reliability of the sensor is further confirmed by enzyme-linked immunosorbent assay (ELISA).
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, People's Republic of China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Ma
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, People's Republic of China.
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Shen
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zeng
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, People's Republic of China.
authors.forename: Hai-Shui
authors.initials: HS
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, People's Republic of China. Electronic address: wanglsh@scut.edu.cn.
authors.forename: Li-Shi
authors.initials: LS
authors.lastname: Wang
mesh_terms: D002138:Calibration; D002244:Carbon / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D005898:Glass / Q000737:chemistry; D016655:HIV Core Protein p24 / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D054802:Molecular Imprinting; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D016655:HIV Core Protein p24; D037742:Nanotubes, Carbon; D011108:Polymers; D002244:Carbon
keywords: Electrochemical sensor; Human immunodeficiency virus p24; Modified electrode; Molecularly imprinted polymers; Multi-walled carbon nanotubes