title: Simple and fast spectral domain algorithm for quantitative phase imaging of living cells with digital holographic microscopy.
abstract: We present a simple and fast phase aberration compensation method in digital holographic microscopy (DHM) for quantitative phase imaging of living cells. By analyzing the frequency spectrum of an off-axis hologram, phase aberrations can be compensated for automatically without fitting or pre-knowledge of the setup and/or the object. Simple and effective computation makes the method suitable for quantitative online monitoring with highly variable DHM systems. Results from automated quantitative phase imaging of living NIH-3T3 mouse fibroblasts demonstrate the effectiveness and the feasibility of the method.
authors.forename: Junwei
authors.initials: J
authors.lastname: Min
authors.forename: Baoli
authors.initials: B
authors.lastname: Yao
authors.forename: Steffi
authors.initials: S
authors.lastname: Ketelhut
authors.forename: Christian
authors.initials: C
authors.lastname: Engwer
authors.forename: Burkhard
authors.initials: B
authors.lastname: Greve
authors.forename: Björn
authors.initials: B
authors.lastname: Kemper
mesh_terms: D000465:Algorithms; D000818:Animals; D002468:Cell Physiological Phenomena; D006696:Holography / Q000379:methods*; D051379:Mice; D008853:Microscopy / Q000379:methods*