title: A lab-on-a-chip for monolith-based preconcentration and electrophoresis separation of phosphopeptides.
abstract: A microdevice combining online preconcentration and separation of phosphopeptides was developed in a glass microchip. An ethylene glycol methacrylate phosphate (EGMP), acrylamide (AM) and bisacrylamide (BAA) based monolith was synthesized within microchannels through a photo-driven process. Morphological investigations revealed a homogeneous monolithic structure composed of uniform nodules (∼0.8 μm), with a large pore volume (0.62 cm3g-1) and sufficiently high specific surface area (34.1 m2g-1). These features make the monolith particularly interesting for preconcentration purposes. Immobilization of Zr4+ions on the phosphate groups present at the poly(EGMP-co-AM-co-BAA) monolith surface leads to immobilized metal affinity chromatography support. This monolith-Zr4+showed a great capacity to capture phosphopeptides. Successful preconcentration and separation of a mixture of ERK2 derived peptides differing only by their phosphorylation degree and sites could be achieved with signal enhancement factors between 340 and 910 after only 7 min of preconcentration. This integrated microdevice represents a novel approach for phosphoproteomic applications.
authors.affiliation: Institut Galien Paris Sud, UMR 8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Univ. Paris-Saclay, 5 rue Jean Baptiste Clément, 92290 Châtenay-Malabry, France. thuy.tran-maignan@u-psud.fr.
authors.forename: Monica
authors.initials: M
authors.lastname: Araya-Farias
authors.affiliation: Institut Galien Paris Sud, UMR 8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Univ. Paris-Saclay, 5 rue Jean Baptiste Clément, 92290 Châtenay-Malabry, France. thuy.tran-maignan@u-psud.fr and Univ. Paris-Est, ICMPE (UMR7182), CNRS, UPEC, Thiais, 94320, France.
authors.forename: Szymon
authors.initials: S
authors.lastname: Dziomba
authors.affiliation: Univ. Paris-Est, ICMPE (UMR7182), CNRS, UPEC, Thiais, 94320, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Carbonnier
authors.affiliation: Univ. Paris-Est, ICMPE (UMR7182), CNRS, UPEC, Thiais, 94320, France.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Guerrouache
authors.affiliation: Institut Galien Paris Sud, UMR 8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Univ. Paris-Saclay, 5 rue Jean Baptiste Clément, 92290 Châtenay-Malabry, France. thuy.tran-maignan@u-psud.fr.
authors.forename: Ichraf
authors.initials: I
authors.lastname: Ayed
authors.affiliation: Institut Galien Paris Sud, UMR 8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Univ. Paris-Saclay, 5 rue Jean Baptiste Clément, 92290 Châtenay-Malabry, France. thuy.tran-maignan@u-psud.fr.
authors.forename: Nacera
authors.initials: N
authors.lastname: Aboud
authors.affiliation: Institut Galien Paris Sud, UMR 8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Univ. Paris-Saclay, 5 rue Jean Baptiste Clément, 92290 Châtenay-Malabry, France. thuy.tran-maignan@u-psud.fr.
authors.forename: Myriam
authors.initials: M
authors.lastname: Taverna
authors.affiliation: Institut Galien Paris Sud, UMR 8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Univ. Paris-Saclay, 5 rue Jean Baptiste Clément, 92290 Châtenay-Malabry, France. thuy.tran-maignan@u-psud.fr.
authors.forename: N Thuy
authors.identifier: http://orcid.org/0000-0002-5845-7922
authors.initials: NT
authors.lastname: Tran
mesh_terms: D002846:Chromatography, Affinity; D004586:Electrophoresis; D005898:Glass; D056656:Lab-On-A-Chip Devices; D010748:Phosphopeptides / Q000302:isolation & purification*
chemical_list: D010748:Phosphopeptides