title: Synergism of Dam, MutH, and MutS in methylation-directed mismatch repair in Escherichia coli.
abstract: DNA mismatch repair (MMR) is a critical mutation surveillance system for recognizing and repairing erroneous insertion, deletion, and disincorporation of base. Major components of mismatch repair system consist of MutH, MutL, and MutS. Dam methylates adenine to distinguish newly synthesized daughter strands from the parent strands. Employing a tyrosine-auxotrophic E. coli FX-11 strain, the mutation frequency can be determined by the number of tyrosine revertants and the cell viability of FX-11 with deficiencies in dam and mismatch repair proteins. This study showed that mutS defect produced a higher mutation frequency than mutH did. Interestingly, double defects in dam and mutS synergistically produced a dramatically higher spontaneous mutation frequency than the summation of mutation frequencies of FX-11 strains with individual deficiency of dam or mutS, suggesting that Dam may work with MutHL to partially accomplish the task of recognizing the mismatch sites to retain partial mismatch repair capacity.
authors.affiliation: Department of Biological Sciences, Emporia State University, 1 Kellogg Circle, Emporia, KS 66801, USA.
authors.forename: Changkun
authors.initials: C
authors.lastname: Hu
authors.affiliation: Department of Biological Sciences, Emporia State University, 1 Kellogg Circle, Emporia, KS 66801, USA; School of Pharmaceutical Sciences & Yunnan Provincial Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, 1168 West Chun Rong Road, Cheng Gong, Kunming, Yunnan 650500, People's Republic of China.
authors.forename: Yunqi
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Biological Sciences, Emporia State University, 1 Kellogg Circle, Emporia, KS 66801, USA.
authors.forename: Huiyun
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Biological Sciences, Emporia State University, 1 Kellogg Circle, Emporia, KS 66801, USA. Electronic address: yyang@emporia.edu.
authors.forename: Yixin
authors.initials: Y
authors.lastname: Yang
mesh_terms: D019175:DNA Methylation / Q000235:genetics*; D053843:DNA Mismatch Repair / Q000235:genetics*; D015254:DNA Modification Methylases / Q000235:genetics / Q000378:metabolism*; D045643:DNA Repair Enzymes / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004706:Endodeoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D051719:MutS DNA Mismatch-Binding Protein / Q000235:genetics / Q000378:metabolism*; D016296:Mutagenesis; D059645:Mutation Rate
chemical_list: D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D015254:DNA Modification Methylases; D004706:Endodeoxyribonucleases; C059682:methyl-directed mismatch repair protein, E coli; D051719:MutS DNA Mismatch-Binding Protein; C497175:MutS protein, E coli; D045643:DNA Repair Enzymes
keywords: DNA methylation-directed mismatch repair; Dam; MutH; MutS