title: Preclinical Study of Antineoplastic Sinoporphyrin Sodium-PDT via In Vitro and In Vivo Models.
abstract: Photodynamic therapy (PDT) investigations have seen stable increases and the development of new photosensitizers is a heated topic. Sinoporphyrin sodium is a new photosensitizer isolated from Photofrin. This article evaluated its anticancer effects by clonogenic assays, MTT assays and xenograft experiments in comparison to Photofrin. The clonogenicity inhibition rates of sinoporphyrin sodium-PDT towards four human cancer cell lines ranged from 85.5% to 94.2% at 0.5 μg/mL under 630 nm irradiation of 30 mW/cm² for 180 s. For MTT assays, the IC50ranges of Photofrin-PDT and sinoporphyrin sodium-PDT towards human cancer cells were 0.3 μg/mL to 5.5 μg/mL and 0.1 μg/mL to 0.8 μg/mL under the same irradiation conditions, respectively. The IC50values of Photofrin-PDT and sinoporphyrin sodium-PDT towards human skin cells, HaCaT, were 10 μg/mL and 1.0 μg/mL, respectively. Esophagus carcinoma and hepatoma xenograft models were established to evaluate the in vivo antineoplastic efficacy. A control group, Photofrin-PDT group (20 mg/kg) and sinoporphyrin sodium group at three doses, 0.5 mg/kg, 1 mg/kg and 2 mg/kg, were set. Mice were injected with photosensitizers 24 h before 60 J 630 nm laser irradiation. The tumor weight inhibition ratio of 2 mg/kg sinoporphyrin sodium-PDT reached approximately 90%. Besides, the tumor growths were significantly slowed down by 2 mg/kg sinoporphyrin sodium-PDT, which was equivalent to 20 mg/kg Photofrin-PDT. In sum, sinoporphyrin sodium-PDT showed great anticancer efficacy and with a smaller dose compared with Photofrin. Further investigations are warranted.
authors.affiliation: New Drug Safety Evaluation Centre, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. shirui87617@foxmail.com.
authors.forename: Rui
authors.initials: R
authors.lastname: Shi
authors.affiliation: New Drug Safety Evaluation Centre, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. kcasper@imm.ac.cn.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: New Drug Safety Evaluation Centre, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. jnkic@163.com.
authors.forename: Zhihuan
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: New Drug Safety Evaluation Centre, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. liwanfang@imm.ac.cn.
authors.forename: Wanfang
authors.initials: W
authors.lastname: Li
authors.affiliation: New Drug Safety Evaluation Centre, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. wangaiping@imm.ac.cn.
authors.forename: Aiping
authors.initials: A
authors.lastname: Wang
authors.affiliation: New Drug Safety Evaluation Centre, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. weijinfeng@imm.ac.cn.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Wei
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D017323:Dihematoporphyrin Ether / Q000737:chemistry; D004353:Drug Evaluation, Preclinical; D004938:Esophageal Neoplasms / Q000188:drug therapy* / Q000473:pathology; D005260:Female; D006801:Humans; D020128:Inhibitory Concentration 50; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects; D054018:Lasers, Excimer; D008027:Light; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011166:Porphyrins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D047368:Tumor Burden / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D017319:Photosensitizing Agents; D011166:Porphyrins; C586896:sinoporphyrin sodium; D017323:Dihematoporphyrin Ether
keywords: MTT assay; clonogenic assay; photodynamic therapy; sinoporphyrin sodium; xenografts model