title: Effect of copper ion and soil humic acid on biodegradation of decabromodiphenyl ether (BDE-209) by Pseudomonas aeruginosa.
abstract: Pseudomonas aeruginosa is a good environmental microorganism capable of degrading decabromodiphenyl ether (BDE-209). This paper studied the effect of Cu2+and humic acid (HA) extracted from e-waste contaminated soils on biodegradation of BDE-209 by P. aeruginosa. The adsorption isotherms of Cu2+on HA, the crude enzyme activity, cell surface morphology, and biodegradation pathway were also investigated. The results showed that BDE-209 biodegradation by P. aeruginosa was inhibited at Cu2+concentrations above 5 mg L-1, but exhibited the best effect at the condition of 40 mg L-1Cu2++ 3 g L-1HA. At the condition of 40 mg L-1Cu2++ 3 g L-1HA, 97.35 ± 2.33% of the initial BDE-209 was degraded after 5 days, debromination efficiency was 72.14 ± 1.89%, crude enzyme activity reached the maximum of 0.519 ± 0.022U g-1protein, cell surface of P. aeruginosa was smooth with normal short-rod shapes, and biodegradation pathway mainly include debromination, hydroxylation, and cleavage of the diphenyl ether bond. It was suggested that soil HA could eliminate the toxic effect of high Cu2+concentrations and biodegradation of BDE-209 was improved by synergistic effect of HA and Cu2+.
authors.affiliation: School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, China.|Institute of Biotechnology, Daqing Branch of Heilongjiang Academy of Science, Daqing, China.|Beijing Key Laboratory for Science and Application of Functional Molecular and Crystalline Materials, University of Science and Technology Beijing, Beijing, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, China.|Beijing Key Laboratory for Science and Application of Functional Molecular and Crystalline Materials, University of Science and Technology Beijing, Beijing, China.
authors.forename: Aijun
authors.identifier: 0000-0002-6261-1013
authors.initials: A
authors.lastname: Gong
authors.affiliation: School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, China.|Beijing Key Laboratory for Science and Application of Functional Molecular and Crystalline Materials, University of Science and Technology Beijing, Beijing, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Qiu
authors.affiliation: School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, China.|Beijing Key Laboratory for Science and Application of Functional Molecular and Crystalline Materials, University of Science and Technology Beijing, Beijing, China.
authors.forename: Jingrui
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, China.|Beijing Key Laboratory for Science and Application of Functional Molecular and Crystalline Materials, University of Science and Technology Beijing, Beijing, China.
authors.forename: Fukai
authors.initials: F
authors.lastname: Li
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001711:Biotransformation; D003300:Copper / Q000378:metabolism*; D055768:Halogenated Diphenyl Ethers / Q000378:metabolism*; D006812:Humic Substances; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism; D014131:Trace Elements / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D055768:Halogenated Diphenyl Ethers; D006812:Humic Substances; D012987:Soil; D012989:Soil Pollutants; D014131:Trace Elements; D003300:Copper; C010902:decabromobiphenyl ether
keywords: 
  Pseudomonas aeruginosa
  ; BDE-209; biodegradation; copper ion; e-waste-contaminated soil; soil humic acid