title: Confocal laser endomicroscopy in head and neck malignancies using FITC-labelled EpCAM- and EGF-R-antibodies in cell lines and tumor biopsies.
abstract: Intraoperative detection of residual malignant cells at tumor margins following excision of primary tumors could help improving surgery and thus patients' outcome. The feasibility of the tumor antigens epidermal growth factor receptor (EGF-R) and epithelial cell adhesion molecule (EpCAM) for antibody-dependent confocal laser scanning endomicroscopy (CLE)-mediated visualization of malignant cells was addressed. Both tumor antigens are highly and frequently expressed in the majority of carcinomas, including head and neck squamous cell carcinomas (HNSCC), and represent prognostic and therapeutic tumor target molecules. FITC-conjugated EGF-R- and EpCAM-specific antibodies served as molecular tools for the detection of antigen-positive cells using the CLE technology. Specificity of both antibodies and their ability to discriminate tumor from non-tumor cells were assessed in vitro with human fibroblasts and PCI-1 HNSCC cell lines, and ex vivo on primary HNSCC samples (n = 11) and healthy mucosa (n = 5). Antigen specificity of the used EpCAM-specific antibody was superior to that of the EGF-R-specific antibody both in vitro and ex vivo (100% vs. 31.25%), and allowed visualization of cellular structures in CLE measurements. These results hold promise for possible future applications in humans.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Klinikum der Universität München, Marchioninistr. 15, 81377, Munich, Germany.
authors.forename: Anna S
authors.initials: AS
authors.lastname: Englhard
authors.affiliation: Laser-Forschungslabor, LIFE-Zentrum, Klinikum der Universität München, Feodor-Lynen-Str. 19, 81377, Munich, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Palaras
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Klinikum der Universität München, Marchioninistr. 15, 81377, Munich, Germany.
authors.forename: Veronika
authors.initials: V
authors.lastname: Volgger
authors.affiliation: Laser-Forschungslabor, LIFE-Zentrum, Klinikum der Universität München, Feodor-Lynen-Str. 19, 81377, Munich, Germany.|Department of Urology, Klinikum der Universität München, Marchioninistr. 15, 81377, Munich, Germany.
authors.forename: Herbert
authors.initials: H
authors.lastname: Stepp
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Klinikum der Universität München, Marchioninistr. 15, 81377, Munich, Germany.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Mack
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Klinikum der Universität München, Marchioninistr. 15, 81377, Munich, Germany.
authors.forename: Darko
authors.initials: D
authors.lastname: Libl
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Klinikum der Universität München, Marchioninistr. 15, 81377, Munich, Germany.|Clinical Cooperation Group "Personalized Radiotherapy of Head and Neck Tumors", Helmholtz Zentrum, München, Germany.
authors.forename: Olivier
authors.initials: O
authors.lastname: Gires
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Klinikum der Universität München, Marchioninistr. 15, 81377, Munich, Germany.
authors.forename: Christian S
authors.initials: CS
authors.lastname: Betz
mesh_terms: D000906:Antibodies / Q000276:immunology* / Q000378:metabolism; D000918:Antibody Specificity; D001706:Biopsy; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D000071858:Epithelial Cell Adhesion Molecule / Q000276:immunology*; D066246:ErbB Receptors / Q000276:immunology*; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism*; D006258:Head and Neck Neoplasms / Q000473:pathology*; D006801:Humans; D007834:Lasers; D018613:Microscopy, Confocal; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: D000906:Antibodies; D000071858:Epithelial Cell Adhesion Molecule; D066246:ErbB Receptors; D016650:Fluorescein-5-isothiocyanate
keywords: CLE; EGF-R; EpCAM; HNSCC; endomicroscopy