title: Synchronized Progression of Prestin Expression and Auditory Brainstem Response during Postnatal Development in Rats.
abstract: Prestin is the motor protein expressed in the cochlear outer hair cells (OHCs) of mammalian inner ear. The electromotility of OHCs driven by prestin is responsible for the cochlear amplification which is required for normal hearing in adult animals. Postnatal expression of prestin and activity of OHCs may contribute to the maturation of hearing in rodents. However, the temporal and spatial expression of prestin in cochlea during the development is not well characterized. In the present study, we examined the expression and function of prestin from the OHCs in apical, middle, and basal turns of the cochleae of postnatal rats. Prestin first appeared at postnatal day 6 (P6) for basal turn, P7 in middle turn, and P9 for apical turn of cochlea. The expression level increased progressively over the next few days and by P14 reached the mature level for all three segments. By comparison with the time course of the development of auditory brainstem response for different frequencies, our data reveal that prestin expression synchronized with the hearing development. The present study suggests that the onset time of hearing may require the expression of prestin and is determined by the mature function of OHCs.
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China; Department of Laboratory Medicines, General Hospital of Guangzhou Military Command of PLA, Guangzhou 510010, China.
authors.forename: Jianfeng
authors.identifier: 0000-0003-1375-5771
authors.initials: J
authors.lastname: Hang
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
authors.forename: Wenlu
authors.identifier: 0000-0002-0083-1950
authors.initials: W
authors.lastname: Pan
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
authors.forename: Aoshuang
authors.identifier: 0000-0001-9434-4768
authors.initials: A
authors.lastname: Chang
authors.affiliation: Affiliated High School of South China Normal University, Guangzhou 510630, China.
authors.forename: Shun
authors.identifier: 0000-0003-3159-1248
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
authors.forename: Cuixian
authors.identifier: 0000-0002-2554-5766
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
authors.forename: Mingyu
authors.identifier: 0000-0001-8519-7989
authors.initials: M
authors.lastname: Fu
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
authors.forename: Jie
authors.identifier: 0000-0001-7882-4462
authors.initials: J
authors.lastname: Tang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D027321:Anion Transport Proteins / Q000096:biosynthesis* / Q000235:genetics; D001933:Brain Stem / Q000254:growth & development* / Q000378:metabolism*; D016057:Evoked Potentials, Auditory, Brain Stem / Q000502:physiology*; D015870:Gene Expression; D008297:Male; D009925:Organ of Corti / Q000254:growth & development / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000076988:Sulfate Transporters
chemical_list: D027321:Anion Transport Proteins; C409207:Slc26a5 protein, rat; D000076988:Sulfate Transporters