title: Extracellular vesicle associated long non-coding RNAs functionally enhance cell viability.
abstract: Cells communicate with one another to create microenvironments and share resources. One avenue by which cells communicate is through the action of exosomes. Exosomes are extracellular vesicles that are released by one cell and taken up by neighbouring cells. But how exosomes instigate communication between cells has remained largely unknown. We present evidence here that particular long non-coding RNA molecules are preferentially packaged into exosomes. We also find that a specific class of these exosome associated non-coding RNAs functionally modulate cell viability by direct interactions with L-lactate dehydrogenase B (LDHB), high-mobility group protein 17 (HMG-17), and CSF2RB, proteins involved in metabolism, nucleosomal architecture and cell signalling respectively. Knowledge of this endogenous cell to cell pathway, those proteins interacting with exosome associated non-coding transcripts and their interacting domains, could lead to a better understanding of not only cell to cell interactions but also the development of exosome targeted approaches in patient specific cell-based therapies.
authors.affiliation: The University of New South Wales, Biotechnology and Biomedical Sciences, Sydney NSW 2052 Australia.
authors.forename: Chris
authors.initials: C
authors.lastname: Hewson
authors.affiliation: The University of New South Wales, Biotechnology and Biomedical Sciences, Sydney NSW 2052 Australia.
authors.forename: David
authors.initials: D
authors.lastname: Capraro
authors.affiliation: The University of New South Wales, Biotechnology and Biomedical Sciences, Sydney NSW 2052 Australia.
authors.forename: Jon
authors.initials: J
authors.lastname: Burdach
authors.affiliation: The University of New South Wales, Biotechnology and Biomedical Sciences, Sydney NSW 2052 Australia.
authors.forename: Noel
authors.initials: N
authors.lastname: Whitaker
authors.affiliation: The University of New South Wales, Biotechnology and Biomedical Sciences, Sydney NSW 2052 Australia; Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA, United States, 92037; City of Hope - Beckman Research Institute, Center for Gene Therapy, Duarte, CA, 91010, United States.
authors.forename: Kevin V
authors.initials: KV
authors.lastname: Morris
keywords: exosomes; extracellular RNA; non-coding RNA; pseudogene; retroelement