title: Establishment of a Novel Simplified Surgical Model of Acute Liver Failure in the Cynomolgus Monkey.
abstract: Models using large animals that are suitable for studying artificial liver support system (ALSS) are urgently needed. Presently available acute liver failure (ALF) models mainly involve pigs or dogs. Establishment of current surgical ALF models (hepatectomy/devascularization) requires either very good surgical skills or multistep processes-even multiple stages of surgery. Therefore, it is necessary to develop a simplified surgical method. Here we report a novel simplified surgical ALF model using cynomolgus monkeys. Six monkeys underwent portal-right renal venous shunt combined with common bile duct ligation and transection (PRRS + CBDLT). Postoperatively, the monkeys had progressively increased listlessness, loss of appetite, and obvious jaundice. Blood biochemistry levels (Amm, ALT, AST, TBiL, DBiL, ALP, LDH, CK, and Cr) and prothrombin time (PT) were significantly increased (allP< 0.01) and albumin (ALB) was markedly reduced (P< 0.01) compared with baseline values. Histological examination of liver specimens on postoperative day 10 revealed cholestasis and inflammation. PRRS + CBDLT produced ALF that closely correlated with clinical situations. Compared with other surgical or drug ALF models, ours was simplified and animals were hemodynamically stable. This model could provide a good platform for further research on ALSS, especially regarding their detoxification functions.
authors.affiliation: Second Department of Hepatobiliary Surgery, Zhujiang Hospital, State Key Laboratory of Organ Failure Research, Co-Innovation Center for Organ Failure Research, Southern Medical University, Guangzhou 510280, China.
authors.forename: Lei
authors.identifier: 0000-0003-1658-7996
authors.initials: L
authors.lastname: Cai
authors.affiliation: Second Department of Hepatobiliary Surgery, Zhujiang Hospital, State Key Laboratory of Organ Failure Research, Co-Innovation Center for Organ Failure Research, Southern Medical University, Guangzhou 510280, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Weng
authors.affiliation: Second Department of Hepatobiliary Surgery, Zhujiang Hospital, State Key Laboratory of Organ Failure Research, Co-Innovation Center for Organ Failure Research, Southern Medical University, Guangzhou 510280, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Feng
authors.affiliation: Second Department of Hepatobiliary Surgery, Zhujiang Hospital, State Key Laboratory of Organ Failure Research, Co-Innovation Center for Organ Failure Research, Southern Medical University, Guangzhou 510280, China.
authors.forename: Guolin
authors.initials: G
authors.lastname: He
authors.affiliation: Second Department of Hepatobiliary Surgery, Zhujiang Hospital, State Key Laboratory of Organ Failure Research, Co-Innovation Center for Organ Failure Research, Southern Medical University, Guangzhou 510280, China.
authors.forename: Jiasheng
authors.initials: J
authors.lastname: Qin
authors.affiliation: Second Department of Hepatobiliary Surgery, Zhujiang Hospital, State Key Laboratory of Organ Failure Research, Co-Innovation Center for Organ Failure Research, Southern Medical University, Guangzhou 510280, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Institute of Regenerative Medicine, Zhujiang Hospital, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Southern Medical University, Guangzhou 510280, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Regenerative Medicine, Zhujiang Hospital, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Southern Medical University, Guangzhou 510280, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Peng
authors.affiliation: Second Department of Hepatobiliary Surgery, Zhujiang Hospital, State Key Laboratory of Organ Failure Research, Co-Innovation Center for Organ Failure Research, Southern Medical University, Guangzhou 510280, China.
authors.forename: Zesheng
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Second Department of Hepatobiliary Surgery, Zhujiang Hospital, State Key Laboratory of Organ Failure Research, Co-Innovation Center for Organ Failure Research, Southern Medical University, Guangzhou 510280, China.
authors.forename: Mingxin
authors.initials: M
authors.lastname: Pan
authors.affiliation: Second Department of Hepatobiliary Surgery, Zhujiang Hospital, State Key Laboratory of Organ Failure Research, Co-Innovation Center for Organ Failure Research, Southern Medical University, Guangzhou 510280, China; Institute of Regenerative Medicine, Zhujiang Hospital, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Southern Medical University, Guangzhou 510280, China.
authors.forename: Yi
authors.identifier: 0000-0003-3525-0133
authors.initials: Y
authors.lastname: Gao
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D006498:Hepatectomy; D006801:Humans; D008099:Liver / Q000473:pathology / Q000601:surgery*; D017114:Liver Failure, Acute / Q000503:physiopathology / Q000601:surgery*; D019164:Liver, Artificial; D008252:Macaca fascicularis / Q000601:surgery*; D008953:Models, Anatomic; D013552:Swine