title: [Targeted imaging ability of a biotinylated imaging probe Biotin-S-S-Rhodol for breast cancer cells in vitro].
abstract: OBJECTIVE
  To investigate performance of a biotinylated imaging probe 3a for targeted imaging of breast cancer cells.
  
  
  METHODS
  Ultraviolet absorption spectrum and fluorescence spectrum were employed to analyze the spectral characteristics of 3a. The fluorescence spectrums of 3a treated with different concentrations of glutathione (GSH) were obtained to determine the sensibility of 3a to GSH. Flow cytometry was used to determine the cellular uptake of 3a by MCF-7 cells, MDA-MB-231 cells and Hs 578Bst cells in the presence or absence of biotin, and the imaging performance of 3a in the 3 cell lines was assessed under an inverted fluorescent microscope. The toxicity of 3a to the cells was evaluated using MTT method.
  
  
  RESULTS
  3a showed the strongest absorption peak at 510 nm, and its fluorescence emission signal was the strongest at 544 nm. As the concentration of GSH increased (0-6 mmol/L), 3a exhibited an increasing fluorescence signal at 544 nm. The cellular uptake of 3a was markedly higher in MDA-MB-231 cells and MCF-7 cells than in Hs 578Bst cells. The imaging studies showed that 3a had a good breast cancer cell-targeting property and produced clear images under fluorescent microscope. MTT assay demonstrated no obvious toxicity of 3a in Hs 578Bst cells even at the concentration of 20 µmol/L, but MCF-7 cells and MDA-MB-231 cells exposed to 2-20 µmol/L 3a showed a lowered cell viability.
  
  
  CONCLUSIONS
  3a is capable of targeted imaging of breast cancer cells mediated by biotin. 3a at the concentration of 2-20 µmol/L has minimal cytotoxicity to normal breast cells but can lower the viability of breast cancer cells.
authors.affiliation: Graduate School of Southern Medical University, Guangzhou 510515, China.E-mail: summerwbj@163.com.
authors.forename: Bi-Juan
authors.initials: BJ
authors.lastname: Wu
authors.forename: Xing-Zi
authors.initials: XZ
authors.lastname: Zhou
authors.forename: Jing-Wen
authors.initials: JW
authors.lastname: Sun
authors.forename: Cui-Wen
authors.initials: CW
authors.lastname: Tan
authors.forename: Xin-Rong
authors.initials: XR
authors.lastname: Wu
mesh_terms: D001710:Biotin / Q000031:analogs & derivatives* / Q000493:pharmacokinetics*; D019921:Biotinylation; D001943:Breast Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D006801:Humans; D061986:MCF-7 Cells; D008856:Microscopy, Fluorescence; D013050:Spectrometry, Fluorescence / Q000379:methods; D014466:Ultraviolet Rays; D044004:Xanthones / Q000493:pharmacokinetics*
chemical_list: D044004:Xanthones; C000607488:rhodol; D001710:Biotin; D005978:Glutathione