title: Contribution of microbiology in the diagnosis of tuberculosis in Castile and León (Spain): Findings of the GRUMICALE 2013 study.
abstract: A retrospective study was conducted by collecting microbiological tuberculosis (TB) data in Castile and León during the year 2013 in order to determine the incidence and distribution of TB, and resistance to the tuberculostatic drug, and compare them with the epidemiological data provided by the Department of Epidemiological Surveillance (SIVE).
  
  
  
  Microbiologists of the 14 hospitals of the Castile and León public health network (GRUMICALE) collected epidemiological, microbiological, and management data from the Microbiology laboratories in the community during the year 2013. A single isolate of Mycobacterium tuberculosis complex (MTC) per patient was considered.
  
  
  
  The study included a total of 270 MTC isolates (an incidence rate of 11.63 cases/100,000 inhab./year). A total of 288 cases of TB (11.43 cases/100,000 inhab. year) were recovered using epidemiological data, which included 243 confirmed, 29 suspected, and 16 as probable cases. Pulmonary TB was predominant, followed a long way off by the pleural TB and the remaining locations. A total of 27,620 samples were processed for mycobacterial detection. Mycobacterial growth was observed in 3.46% of automated fluid cultures, and 50.37% were positive by direct staining of the smear. Resistance to one tuberculostatic drug, mostly to isoniazid, was observed in 16 (5.92%) isolates of Mycobacterium tuberculosis (MT). The province with greater incidence and number of isolates was León (24.23 cases/100,000 inhab./year), with the highest being observed in El Bierzo health area (30.46 cases/100,000 inhab./year).
  
  
  
  An adequate collection of microbiological information is essential to determine the epidemiology of TB in our region.
authors.affiliation: Unidad de Microbiología, Hospital El Bierzo, Ponferrada, León, España. Electronic address: ramirofos@yahoo.es.
authors.forename: Ramiro
authors.initials: R
authors.lastname: López-Medrano
authors.affiliation: Servicio de Microbiología, Complejo Asistencial Universitario de León (CAULE), León, España.
authors.forename: Teresa
authors.initials: T
authors.lastname: Nebreda-Mayoral
authors.affiliation: Sección de Microbiología, Complejo Hospitalario de Zamora, Zamora, España.
authors.forename: M Fé
authors.initials: MF
authors.lastname: Brezmes-Valdivieso
authors.affiliation: Sección de Microbiología, Hospital de Soria, Soria, España.
authors.forename: Susana
authors.initials: S
authors.lastname: García-de Cruz
authors.affiliation: Servicio de Microbiología, Hospital Clínico Universitario de Valladolid, Valladolid, España.
authors.forename: Begoña
authors.initials: B
authors.lastname: Nogueira-González
authors.affiliation: Sección de Microbiología, Hospital de Ávila, Ávila, España.
authors.forename: Rafael
authors.initials: R
authors.lastname: Sánchez-Arroyo
authors.affiliation: Sección de Microbiología, Hospital de Palencia, Palencia, España.
authors.forename: Almudena
authors.initials: A
authors.lastname: Tinajas-Puertas
authors.affiliation: Servicio de Microbiología, Hospital Universitario de Salamanca, Salamanca, España.
authors.forename: Nieves
authors.initials: N
authors.lastname: Gutiérrez-Zufiaurre
authors.affiliation: Sección de Microbiología, Complejo Asistencial de Burgos, Burgos, España.
authors.forename: Cristina
authors.initials: C
authors.lastname: Labayru-Echeverría
authors.affiliation: Sección de Microbiología, Hospital de Segovia, Segovia, España.
authors.forename: Susana
authors.initials: S
authors.lastname: Hernando-Real
authors.affiliation: Sección de Microbiología, Hospital Universitario Río Hortega, Valladolid, España.
authors.forename: Luis
authors.initials: L
authors.lastname: López-Urrutia
authors.affiliation: Unidad de Investigación, Complejo Asistencial Universitario de León (CAULE), León, España.
authors.forename: Octavio
authors.initials: O
authors.lastname: Rivero-Lezcano
authors.affiliation: Sección de Microbiología, Hospital Santiago Apóstol, Miranda de Ebro, Burgos, España.
authors.forename: Belén
authors.initials: B
authors.lastname: Ullivarri-Francia
authors.affiliation: Sección de Microbiología, Hospital Santos Reyes, Aranda de Duero, Burgos, España.
authors.forename: Raquel
authors.initials: R
authors.lastname: Rodríguez-Tarazona
authors.affiliation: Sección de Microbiología, Hospital de Medina del Campo, Medina del Campo, Valladolid, España.
authors.forename: Isabel
authors.initials: I
authors.lastname: Antolín-Ayala
mesh_terms: D001431:Bacteriological Techniques; D024881:Drug Resistance, Bacterial; D006801:Humans; D015994:Incidence; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification; D012189:Retrospective Studies; D013030:Spain / Q000453:epidemiology; D014376:Tuberculosis / Q000175:diagnosis* / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology
keywords: Castile and León; Castilla y León; Epidemiología de la tuberculosis; Microbiología de la tuberculosis; Tuberculosis epidemiology; Tuberculosis microbiology