title: Assembly of a nucleus-like structure during viral replication in bacteria.
abstract: We observed the assembly of a nucleus-like structure in bacteria during viral infection. Using fluorescence microscopy and cryo-electron tomography, we showed that Pseudomonas chlororaphis phage 201φ2-1 assembled a compartment that separated viral DNA from the cytoplasm. The phage compartment was centered by a bipolar tubulin-based spindle, and it segregated phage and bacterial proteins according to function. Proteins involved in DNA replication and transcription localized inside the compartment, whereas proteins involved in translation and nucleotide synthesis localized outside. Later during infection, viral capsids assembled on the cytoplasmic membrane and moved to the surface of the compartment for DNA packaging. Ultimately, viral particles were released from the compartment and the cell lysed. These results demonstrate that phages have evolved a specialized structure to compartmentalize viral replication.
authors.affiliation: Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093, USA.
authors.forename: Vorrapon
authors.initials: V
authors.lastname: Chaikeeratisak
authors.affiliation: Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093, USA.
authors.forename: Katrina
authors.initials: K
authors.lastname: Nguyen
authors.affiliation: Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093, USA.
authors.forename: Kanika
authors.initials: K
authors.lastname: Khanna
authors.affiliation: Howard Hughes Medical Institute (HHMI) and the Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA 94158, USA.
authors.forename: Axel F
authors.initials: AF
authors.lastname: Brilot
authors.affiliation: Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093, USA.
authors.forename: Marcella L
authors.initials: ML
authors.lastname: Erb
authors.affiliation: Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093, USA.
authors.forename: Joanna K C
authors.initials: JK
authors.lastname: Coker
authors.affiliation: Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093, USA.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Vavilina
authors.affiliation: Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093, USA.
authors.forename: Gerald L
authors.initials: GL
authors.lastname: Newton
authors.affiliation: Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA 92093, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Buschauer
authors.affiliation: Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093, USA.
authors.forename: Kit
authors.initials: K
authors.lastname: Pogliano
authors.affiliation: Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA 92093, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Villa
authors.affiliation: Howard Hughes Medical Institute (HHMI) and the Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA 94158, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Agard
authors.affiliation: Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093, USA. jpogliano@ucsd.edu.
authors.forename: Joe
authors.initials: J
authors.lastname: Pogliano
mesh_terms: D002213:Capsid / Q000378:metabolism; D036022:Capsid Proteins / Q000096:biosynthesis / Q000235:genetics; D020285:Cryoelectron Microscopy; D003593:Cytoplasm / Q000648:ultrastructure / Q000821:virology; D004279:DNA, Viral / Q000096:biosynthesis; D008856:Microscopy, Fluorescence; D017105:Pseudomonas Phages / Q000235:genetics / Q000502:physiology*; D000070096:Pseudomonas chlororaphis / Q000648:ultrastructure / Q000821:virology*; D014158:Transcription, Genetic; D019065:Virus Assembly
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral