title: Iodometric and Molecular Detection of ESBL Production Among Clinical Isolates of E. coli Fingerprinted by ERIC-PCR: The First Egyptian Report Declares the Emergence of E. coli O25b-ST131clone Harboring blaGES.
abstract: The extensive use of β-lactam antibiotics has led to emergence and spread of extended-spectrum β-lactamases (ESBLs). This study was conducted to investigate the prevalence of 7 different ESBL genes (blaTEM, blaSHV, blaCTX-M, blaVEB, blaPER, blaGES, and blaOXA-10) and O25b-ST131 high-risk clone among 61 clinical isolates of Escherichia coli. Also, one broad-spectrum β-lactamase (blaOXA-1) was investigated. This study was also constructed to evaluate iodometric overlay method in detection of ESBL production. Phenotypic identification of E. coli isolates using API 20E revealed 18 distinct biotypes. DNA fingerprinting using enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) differentiated all isolates into 2 main phylogenetic groups with 60 distinct genetic profiles. Elevated values of minimal inhibitory concentration (MIC)50and MIC90for third- and fourth-generation cephalosporins were observed. Phenotypic tests revealed that 85.24% of isolates were ESBL producers. The incidence rates of blaTEM, blaSHV, blaCTX-M, blaGES, blaOXA-1, and blaOXA-10among E. coli ESBL producer phenotype were 69.23%, 25%, 96.15%, 3.85%, 11.54%, and 48%, respectively. On the other hand, blaVEBand blaPERwere not detected. Sequencing of blaTEMand blaSHVrevealed that blaTEM-214and blaSHV-11were the most prevalent variants. Group characterization of blaCTX-Mrevealed that blaCTX-M-1was the most prevalent group of blaCTX-Mfamily. It was found that 30.77% of E. coli ESBL producers belonged to O25b-ST131 clone harboring blaCTX-M-15. This study concluded that iodometric overlay method was 100% sensitive in detection of ESBL production. To our knowledge, this is the first Egyptian study that declares the emergence of E. coli O25b-ST131 harboring blaGES.
authors.affiliation: 1 Department of Microbiology and Immunology, Faculty of Pharmacy, Misr University for Science and Technology , Cairo, Egypt .|2 Department of Pharmaceutical Microbiology, College of Pharmacy, Taif University , Taif, Saudi Arabia .
authors.forename: Mohamed F
authors.initials: MF
authors.lastname: El-Badawy
authors.affiliation: 1 Department of Microbiology and Immunology, Faculty of Pharmacy, Misr University for Science and Technology , Cairo, Egypt .
authors.forename: Wael M
authors.initials: WM
authors.lastname: Tawakol
authors.affiliation: 3 Department of Clinical Pharmacy, College of Pharmacy, Taif University , Taif, Saudi Arabia .
authors.forename: Ibrahim A
authors.initials: IA
authors.lastname: Maghrabi
authors.affiliation: 4 Department of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar University , Cairo, Egypt .
authors.forename: Moselhy S
authors.initials: MS
authors.lastname: Mansy
authors.affiliation: 2 Department of Pharmaceutical Microbiology, College of Pharmacy, Taif University , Taif, Saudi Arabia .|5 Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Tanta University , Tanta, Egypt .
authors.forename: Mohamed M
authors.initials: MM
authors.lastname: Shohayeb
authors.affiliation: 4 Department of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar University , Cairo, Egypt .
authors.forename: Mohammed S
authors.initials: MS
authors.lastname: Ashour
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002648:Child; D002675:Child, Preschool; D004534:Egypt; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008826:Microbial Sensitivity Tests / Q000379:methods; D008875:Middle Aged; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D055815:Young Adult; D001618:beta-Lactamases / Q000235:genetics*; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; D047090:beta-Lactams; D001618:beta-Lactamases
keywords: E. coli; ERIC-PCR; ESBLs; O25b-ST131 clone; blaGES; iodometric overlay