title: Effects of negatively and positively charged Ti metal surfaces on ceramic coating adhesion and cell response.
abstract: This manuscript reports an evaluation of the effects of simple chemical-heat treatments on the deposition of different ceramic coatings, i.e., TiO2, CaTiO3and CaP, on commercially pure titanium (cp-Ti) and Ti6Al4V and the influence of the coatings on cells interaction with the surfaces. The ceramic materials were prepared by the sol-gel method and the coating adhesion was analyzed by pull-off bending tests. The wettability of positively or negatively charged surfaces was characterized by contact angle measurements, which also enabled the calculation of the surface free energy through the polar-apolar liquids approach. Both acid and alkaline treatments activated the cp-Ti, whereas Ti6Al4V was only activated by the alkaline treatment. Such treatment led to increased hydrophilicity with inhibition of the fibroblastic response on Ti6Al4V. On the other hand, osteoblastic cells adhered to and proliferated on the positively and negatively charged surfaces. The maximum adhesion strength (~ 3400 N) was obtained with a negative Ti6Al4V-CaTiO3-CaP multilayer surface.
authors.affiliation: São Carlos Institute of Physics, University of São Paulo, USP, PO Box 369, 1356-6590, São Carlos, SP, Brazil. rodneymn@ifsc.usp.br.|Laboratory of Technologycal Plasmas, Universidade Estadual Paulista, UNESP, Sorocaba, SP, Brazil. rodneymn@ifsc.usp.br.
authors.forename: Rodney Marcelo
authors.initials: RM
authors.lastname: do Nascimento
authors.affiliation: Departamento de Microbiologia, Universidade Estadual Paulista, UNESP, Botucatu, SP, Brazil.
authors.forename: Vanessa Rafaela
authors.initials: VR
authors.lastname: de Carvalho
authors.affiliation: Departamento de Estatística Matemática Aplicada e Computação, Universidade Estadual Paulista, UNESP, Rio Claro, SP, Brazil.
authors.forename: José Silvio
authors.initials: JS
authors.lastname: Govone
authors.affiliation: São Carlos Institute of Physics, University of São Paulo, USP, PO Box 369, 1356-6590, São Carlos, SP, Brazil.
authors.forename: Antônio Carlos
authors.initials: AC
authors.lastname: Hernandes
authors.affiliation: Laboratory of Technologycal Plasmas, Universidade Estadual Paulista, UNESP, Sorocaba, SP, Brazil.
authors.forename: Nilson Cristino
authors.initials: NC
authors.lastname: da Cruz
mesh_terms: D000497:Alloys; D000818:Animals; D015415:Biomarkers / Q000737:chemistry; D017610:Calcium Compounds / Q000737:chemistry; D002448:Cell Adhesion; D049109:Cell Proliferation; D002516:Ceramics / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry; D005347:Fibroblasts / Q000187:drug effects*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D010087:Oxides / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011817:Rabbits; D013314:Stress, Mechanical; D013499:Surface Properties; D000267:Tissue Adhesions; D014025:Titanium / Q000737:chemistry*; D017155:Wettability
chemical_list: D000497:Alloys; D015415:Biomarkers; D017610:Calcium Compounds; D020099:Coated Materials, Biocompatible; D010087:Oxides; D011108:Polymers; C059910:perovskite; C031462:titanium alloy (TiAl6V4); D017886:Durapatite; D014025:Titanium