title: Custom database development and biomarker discovery methods for MALDI-TOF mass spectrometry-based identification of high-consequence bacterial pathogens.
abstract: A high-quality custom database of MALDI-TOF mass spectral profiles was developed with the goal of improving clinical diagnostic identification of high-consequence bacterial pathogens. A biomarker discovery method is presented for identifying and evaluating MALDI-TOF MS spectra to potentially differentiate biothreat bacteria from less-pathogenic near-neighbour species.
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.
authors.forename: Dobryan M
authors.initials: DM
authors.lastname: Tracz
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.
authors.forename: Andrea D
authors.initials: AD
authors.lastname: Tyler
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.
authors.forename: Ian
authors.initials: I
authors.lastname: Cunningham
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.
authors.forename: Kym S
authors.initials: KS
authors.lastname: Antonation
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada; University of Manitoba, Department of Medical Microbiology and Infectious Diseases, Winnipeg, Manitoba R3E 0J9, Canada. Electronic address: Cindi.Corbett@phac-aspc.gc.ca.
authors.forename: Cindi R
authors.initials: CR
authors.lastname: Corbett
mesh_terms: D001407:Bacillus / Q000235:genetics / Q000302:isolation & purification; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*; D001424:Bacterial Infections / Q000175:diagnosis / Q000382:microbiology; D001426:Bacterial Proteins / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D054045:Biological Warfare Agents; D015415:Biomarkers / Q000032:analysis*; D002002:Brucella / Q000235:genetics / Q000302:isolation & purification; D030561:Databases, Nucleic Acid; D006801:Humans; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods* / Q000592:standards; D015007:Yersinia / Q000235:genetics / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D054045:Biological Warfare Agents; D015415:Biomarkers
keywords: Bioterrorism bacterial pathogens; Biothreat; Genus Yersinia, Bacillus, Brucella; MALDI-TOF mass spectrometry