title: Co-Expression of Putative Cancer Stem Cell Markers CD44 and CD133 in Prostate Carcinomas.
abstract: Cancer stem cells (CSCs) are the main players of prostate tumorigenesis thus; characterization of CSCs can pave the way for understanding the early detection, drug resistance, metastasis and relapse. The current study was conducted to evaluate the expression level and clinical significance of the potential CSC markers CD44 and CD133 in a series of prostate tissues. One hundred and forty eight prostate tissues composed of prostate cancer (PCa), high-grade prostatic intraepithelial neoplasia (HGPIN), and benign prostate hyperplasia (BPH) were immunostained for the putative CSC markers CD44 and CD133. Subsequently, the correlation between the expression of these markers and the clinicopathological variables was examined. A higher level of CD44 expression was observed in 42% of PCa, 57% of HGPIN, and 42% BPH tissues. In the case of CD133 expression PCa, HGPIN, and BPH samples demonstrated high immunoreactivity in 46%, 43%, and 42% of cells, respectively. Statistical analysis showed an inverse significant correlation between CD44 expression with Gleason score of PCa (P = 0.02), while no significant correlation was observed between CD133 expression and clinicopathological parameters. A significant reciprocal correlation was observed between the expression of two putative CSC markers CD44 and CD133 in PCa specimens while not indicating clinical significance. Further clinical investigation is required to consider these markers as targets of new therapeutic strategies for PCa.
authors.affiliation: Oncopathology Research Center, Iran University of Medical Sciences (IUMS), Hemmat Street (Highway), Next to Milad Tower, Tehran, 14496-14530, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Kalantari
authors.affiliation: Oncopathology Research Center, Iran University of Medical Sciences (IUMS), Hemmat Street (Highway), Next to Milad Tower, Tehran, 14496-14530, Iran. mojgan_asgari@yahoo.com.|Department of Pathology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran. mojgan_asgari@yahoo.com.|Department of Pathology, Hasheminejad Urology-Nephrology Center, Iran University of Medical Sciences, Tehran, Iran. mojgan_asgari@yahoo.com.
authors.forename: Mojgan
authors.initials: M
authors.lastname: Asgari
authors.affiliation: Department of Pathology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Seyedehmoozhan
authors.initials: S
authors.lastname: Nikpanah
authors.affiliation: Department of Pathology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Naghme
authors.initials: N
authors.lastname: Salarieh
authors.affiliation: Department of Cellular, Anatomical and Physiological Sciences, Faculty of Medicine, University of British Columbia (UBC), Vancouver, BC, Canada.
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Asadi Lari
authors.affiliation: Oncopathology Research Center, Iran University of Medical Sciences (IUMS), Hemmat Street (Highway), Next to Milad Tower, Tehran, 14496-14530, Iran. Zahra.madjd@yahoo.com.|Department of Pathology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran. Zahra.madjd@yahoo.com.|Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran. Zahra.madjd@yahoo.com.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Madjd
mesh_terms: D000071916:AC133 Antigen / Q000032:analysis / Q000096:biosynthesis*; D000230:Adenocarcinoma / Q000473:pathology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D006801:Humans; D018960:Hyaluronan Receptors / Q000032:analysis / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000473:pathology*; D011470:Prostatic Hyperplasia; D019048:Prostatic Intraepithelial Neoplasia / Q000473:pathology; D011471:Prostatic Neoplasms / Q000473:pathology*
chemical_list: D000071916:AC133 Antigen; D014408:Biomarkers, Tumor; C497483:CD44 protein, human; D018960:Hyaluronan Receptors; C000606023:PROM1 protein, human
keywords: CD133; CD44; Immunohistochemistry; Prostate cancer; Tissue microarray