title: Decreased expression of LncRNA SRA1 in hepatocellular carcinoma and its clinical significance.
abstract: BACKGROUND
  Hepatocellular carcinoma (HCC), is an extremely aggressive malignancy with poor prognosis and high fatality rates worldwide. Accumulating evidence indicated that novel biomarkers are required to get a better understanding of the biological mechanisms of HCC. SRA1, a long non-coding RNA (lncRNA), serves as a critical regulator in several cancers. However, the association between SRA1 expression and tumorigenesis in HCC tissues remains unclear.
  
  
  OBJECTIVE
  In the present study, we evaluated the expression of SRA1 in HCC and its clinical association.
  
  
  METHODS
  The expression levels of SRA1 in 67 pairs of cancer tissues and adjacent normal tissues from HCC patients were detected using quantitative real-time PCR. Expression of SRA1 in HCC cell lines compared with normal human hepatocyte cell lines was also measured. Finally, the potential associations between its level in HCC tissues and the clinicopathological parameters were analyzed as well.
  
  
  RESULTS
  The results indicated that the expression levels of SRA1 in HCC were remarkably decreased, compared with matched normal tissues (P< 0.001). Levels of SRA1 in HCC cell lines were also significantly decreased than that in normal human hepatocyte cell line L-02. Additionally, the levels of SRA1 were significantly associated with tumor size (P= 0.020) and serum GLU level (P= 0.046).
  
  
  CONCLUSIONS
  This study highlighted that SRA1 was downregulated in HCC and might serve as a tumor suppressor in HCC, which laid a solid foundation for future research.
authors.forename: Ping
authors.initials: P
authors.lastname: Luo
authors.forename: Wei
authors.initials: W
authors.lastname: Jing
authors.forename: Man
authors.initials: M
authors.lastname: Zhu
authors.forename: Nan-Di
authors.initials: ND
authors.lastname: Li
authors.forename: Hu
authors.initials: H
authors.lastname: Zhou
authors.forename: Ming-Xia
authors.initials: MX
authors.lastname: Yu
authors.forename: Chun-Zi
authors.initials: CZ
authors.lastname: Liang
authors.forename: Jian-Cheng
authors.initials: JC
authors.lastname: Tu
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000235:genetics*; D012307:Risk Factors; D047368:Tumor Burden
chemical_list: D014408:Biomarkers, Tumor; D002352:Carrier Proteins; D062085:RNA, Long Noncoding; C548898:steroid receptor RNA activator, human
keywords: HCC; Long non-coding RNA; SRA1; down-expression