title: Biological properties of citral and its potential protective effects against cytotoxicity caused by aspirin in the IEC-6 cells.
abstract: Citral, 3,7-dimethyl-2,6-octadienal, is a key component of several essential oils extracted from lemon-scented herbal plants. The present study was designed to investigate the antioxidant activities of citral and assess its possible protective effects against aspirin-induced toxicity in vitro. We used IEC-6 cells (rat small intestine epithelial cells). The antioxidant activities were determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH), β-carotene/linoleic acid and Ferric reducing antioxidant power (FRAP). Cytotoxicity was evaluated by cell viability, anti-oxidant enzyme activities, malondialdehyde (MDA) production and by the expression of MAPKs (Mitogen-Activated Protein Kinases) pathways. According to results, citral showed an important antioxidant activity. It inhibited the oxidation of linoleic acid, a moderate DPPH was found and it showed a Ferric reducing antioxidant potential with an EC50value of 125±28.86μg/mL. Then, the co-treatment of aspirin with citral significantly decreased the aspirin-induced cell death, and the MDA level. It modulated the superoxide dismutase (SOD) and glutathione (GSH) activities. Also, the activation of MAPKs was attenuated by citral. These findings suggest that citral can protect IEC-6 cells against aspirin-induced oxidative stress that may help to discover new chemicals out of natural antioxidant substances.
authors.affiliation: Physiology Department-EA1274, Faculty of Medecine and Health Sciences, University of Western Brittany, 22 Avenue Camille Desmoulins, CS 93837, 29238 Brest Cedex 3, France; Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences of Sfax, Road Soukra km 3.5, PB no 1171-3000, Sfax, Tunisia; Laboratory of Animal Eco Physiology, Faculty of Sciences of Gafsa, 2112, Tunisia. Electronic address: bouzenna.hafsia@hotmail.fr.
authors.forename: Hafsia
authors.initials: H
authors.lastname: Bouzenna
authors.affiliation: Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences of Sfax, Road Soukra km 3.5, PB no 1171-3000, Sfax, Tunisia; Laboratory of Animal Eco Physiology, Faculty of Sciences of Gafsa, 2112, Tunisia.
authors.forename: Najla
authors.initials: N
authors.lastname: Hfaiedh
authors.affiliation: Physiology Department-EA1274, Faculty of Medecine and Health Sciences, University of Western Brittany, 22 Avenue Camille Desmoulins, CS 93837, 29238 Brest Cedex 3, France.
authors.forename: Marie-Agnès
authors.initials: MA
authors.lastname: Giroux-Metges
authors.affiliation: Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences of Sfax, Road Soukra km 3.5, PB no 1171-3000, Sfax, Tunisia.
authors.forename: Abdelfattah
authors.initials: A
authors.lastname: Elfeki
authors.affiliation: Physiology Department-EA1274, Faculty of Medecine and Health Sciences, University of Western Brittany, 22 Avenue Camille Desmoulins, CS 93837, 29238 Brest Cedex 3, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Talarmin
mesh_terms: D000080462:Acyclic Monoterpenes; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001241:Aspirin / Q000009:adverse effects*; D001713:Biphenyl Compounds / Q000494:pharmacology; D002460:Cell Line; D005978:Glutathione / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases; D039821:Monoterpenes / Q000494:pharmacology*; D009822:Oils, Volatile / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D010851:Picrates / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000080462:Acyclic Monoterpenes; D000975:Antioxidants; D001713:Biphenyl Compounds; D039821:Monoterpenes; D009822:Oils, Volatile; D010851:Picrates; D010936:Plant Extracts; D020011:Protective Agents; D008315:Malondialdehyde; C004931:1,1-diphenyl-2-picrylhydrazyl; D013482:Superoxide Dismutase; D020928:Mitogen-Activated Protein Kinases; D005978:Glutathione; D001241:Aspirin; C007076:citral
keywords: Aspirin; Citral; Cytotoxicity; IEC-6 cells; MAPK; Oxidative stress