title: The effect of N-acetylcysteine supplementation on serum homocysteine levels and hepatic and renal oxidative stress in homocysteine thiolactone-treated rats.
abstract: The effect of N-acetylcysteine (NAC) (1 g/kg body weight/day) on serum homocysteine (Hcy) levels, insulin resistance (IR), and hepatic and renal prooxidant-antioxidant balance was evaluated in rats treated with homocysteine thiolactone (HcyT) (500 mg/kg body weight/day for 6 weeks). Reactive oxygen species (ROS), malondialdehyde (MDA), glutathione, ferric reducing antioxidant power, and superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were determined in the liver and kidney. HcyT elevated serum Hcy levels and caused IR, but liver and kidney function tests remained unchanged. HcyT increased ROS and MDA without any change in hepatic antioxidants, but it elevated renal SOD and GSH-Px activities. NAC decreased serum Hcy, hepatic and renal ROS and MDA levels, and renal SOD and GSH-Px activities in rats with high Hcy levels. However, it did not ameliorate IR. Our results indicate that NAC supplementation may be effective in decreasing Hcy levels and Hcy-induced hepatic and renal oxidative stress.
authors.affiliation: a Department of Biochemistry , Istanbul Medical Faculty, Istanbul University , Istanbul , Turkey.
authors.forename: Gamze
authors.initials: G
authors.lastname: Kondakçı
authors.affiliation: a Department of Biochemistry , Istanbul Medical Faculty, Istanbul University , Istanbul , Turkey.
authors.forename: A Fatih
authors.initials: AF
authors.lastname: Aydın
authors.affiliation: a Department of Biochemistry , Istanbul Medical Faculty, Istanbul University , Istanbul , Turkey.
authors.forename: Semra
authors.initials: S
authors.lastname: Doğru-Abbasoğlu
authors.affiliation: a Department of Biochemistry , Istanbul Medical Faculty, Istanbul University , Istanbul , Turkey.
authors.forename: Müjdat
authors.initials: M
authors.lastname: Uysal
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D019587:Dietary Supplements; D016166:Free Radical Scavengers / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D006710:Homocysteine / Q000031:analogs & derivatives* / Q000097:blood* / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D007677:Kidney Function Tests; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D008111:Liver Function Tests; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D016877:Oxidants / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D011837:Radiation-Protective Agents / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D016877:Oxidants; D011837:Radiation-Protective Agents; D006710:Homocysteine; D008315:Malondialdehyde; C007957:homocysteine thiolactone; D005978:Glutathione; D000111:Acetylcysteine
keywords: Homocysteine thiolactone; N-acetylcysteine; insulin resistance; kidney; liver; oxidative stress