title: Comparison of potential higher order reference methods for total haemoglobin quantification-an interlaboratory study.
abstract: The total haemoglobin (Hb) concentration in blood is one of the most frequently measured analytes in clinical medicine because of its significance for evaluating the health state of a human. The spectrophotometric cyanmethaemoglobin (HiCN) method is the internationally accepted conventional reference method to determine this biomarker. It is frequently used in clinical routine diagnostics but is not traceable to the International System of Units and thus does not meet highest metrological demands. A further critical issue is the toxicity of the necessary potassium cyanide. Different methods to solve these problems are reported here. They all were validated against the HiCN method in an interlaboratory comparison by measuring the total Hb concentration present in the certified reference material JCCRM 912-2M. Methods considered were the spectrophotometric alkaline haematin detergent (AHD) method as well as several isotope dilution (ID)-based approaches. The latter include inductively coupled plasma mass spectrometry (ICP-MS), species-specific (SS) ICP-MS, organic MS and Raman spectrometry. Graphical abstract ᅟ.
authors.affiliation: Physikalisch-Technische Bundesanstalt (PTB), Bundesallee 100, 38116, Braunschweig, Germany. Claudia.Frank@ptb.de.
authors.forename: Claudia
authors.initials: C
authors.lastname: Frank
authors.affiliation: Physikalisch-Technische Bundesanstalt (PTB), Bundesallee 100, 38116, Braunschweig, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Brauckmann
authors.affiliation: Laboratoire National de Métrologie et d'essais (LNE), 1, Rue Gaston Boissier, 75784, Paris Cedex 15, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Palos
authors.affiliation: Physikalisch-Technische Bundesanstalt (PTB), Bundesallee 100, 38116, Braunschweig, Germany.
authors.forename: Cristian G
authors.initials: CG
authors.lastname: Arsene
authors.affiliation: Physikalisch-Technische Bundesanstalt (PTB), Abbestraße 2-12, 10587, Berlin, Germany.
authors.forename: Joerg
authors.initials: J
authors.lastname: Neukammer
authors.affiliation: Laboratoire National de Métrologie et d'essais (LNE), 1, Rue Gaston Boissier, 75784, Paris Cedex 15, France.
authors.forename: Maria Estela
authors.initials: ME
authors.lastname: Del Castillo Busto
authors.affiliation: Physikalisch-Technische Bundesanstalt (PTB), Bundesallee 100, 38116, Braunschweig, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Zakel
authors.affiliation: Physikalisch-Technische Bundesanstalt (PTB), Bundesallee 100, 38116, Braunschweig, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Swart
authors.affiliation: Physikalisch-Technische Bundesanstalt (PTB), Bundesallee 100, 38116, Braunschweig, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Güttler
authors.affiliation: Physikalisch-Technische Bundesanstalt (PTB), Bundesallee 100, 38116, Braunschweig, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Stosch
mesh_terms: D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D007753:Laboratories / Q000458:organization & administration*; D013058:Mass Spectrometry; D013059:Spectrum Analysis, Raman
chemical_list: D006454:Hemoglobins
keywords: Haemoglobin; HiCN; Isotope dilution; Mass spectrometry; Raman spectroscopy; Reference method