title: Isolation and identification of a humanTRPV1 activating compound from soy sauce.
abstract: Transient receptor potential vanilloid 1 (TRPV1) was identified as a receptor of capsaicin, which is a pungent ingredient in hot red peppers. Due to its relevance for nociception, a physiological and pharmacological study of TRPV1 has also been developed. Therefore, it is important to enrich scientific knowledge regarding the TRPV1 activating or inhibiting compounds. In this study, we fractionated soy sauce based on the human TRPV1 (hTRPV1) activity using column chromatography and purified 5-(9H-pyrido[3,4-b]indol-1-yl)-2-furanmethanol (perlolyrine) as an hTRPV1-activating compound. Additionally, perlolyrine activates the human transient receptor potential ankyrin 1 (hTRPA1). The EC50of hTRPV1 and hTRPA1 were 2.87 and 1.67 μmol L-1, respectively. HPLC quantification of soy sauces showed that they contain 2.22-12.13 μmol L-1of perlolyrine. The sensory evaluation revealed that perlolyrine has taste modification effect. The results of this study, for the first time, suggest that perlolyrine induces the activation of hTRPV1 and hTRPA1.
authors.affiliation: a Manufacturing Division , Yamasa Corporation , Choshi , Japan.
authors.forename: Mayu
authors.initials: M
authors.lastname: Oshida
authors.affiliation: a Manufacturing Division , Yamasa Corporation , Choshi , Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Matsuura
authors.affiliation: b School of Food and Nutritional Sciences , University of Shizuoka , Shizuoka , Japan.
authors.forename: Shinnosuke
authors.initials: S
authors.lastname: Hotta
authors.affiliation: a Manufacturing Division , Yamasa Corporation , Choshi , Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Watanabe
authors.affiliation: a Manufacturing Division , Yamasa Corporation , Choshi , Japan.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Mogi
authors.affiliation: b School of Food and Nutritional Sciences , University of Shizuoka , Shizuoka , Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Watanabe
mesh_terms: D002243:Carbolines / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology*; D005663:Furans / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology*; D057809:HEK293 Cells; D006801:Humans; D045730:Soy Foods; D050916:TRPV Cation Channels / Q000378:metabolism*; D013649:Taste
chemical_list: D002243:Carbolines; D005663:Furans; D050916:TRPV Cation Channels; C494482:TRPV1 protein, human; C055781:perlolyrine
keywords: 5-(9H-pyrido[3,4-b]indol-1-yl)-2-furanmethanol; hTRPA1; hTRPV1; perlolyrine; soy sauce