title: Chitosan cross-linked with poly(ethylene glycol)dialdehyde via reductive amination as effective controlled release carriers for oral protein drug delivery.
abstract: The covalently cross-linked chitosan-poly(ethylene glycol)1540derivatives have been developed as a controlled release system with potential for the delivery of protein drug. The swelling characteristics of the hydrogels based on these derivatives as the function of different PEG content and the release profiles of a model protein (bovine serum albumin, BSA) from the hydrogels were evaluated in simulated gastric fluid with or without enzyme in order to simulate the gastrointestinal tract conditions. The derivatives cross-linked with difunctional PEG1540-dialdehyde via reductive amination can swell in alkaline pH and remain insoluble in acidic medium. The cumulative release amount of BSA was relatively low in the initial 2h and increased significantly at pH 7.4 with intestinal lysozyme for additional 12h. The results proved that the release-and-hold behavior of the cross-linked CS-PEG1540H-CS hydrogel provided a swell and intestinal enzyme controlled release carrier system, which is suitable for oral protein drug delivery.
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Zi-Wei
authors.initials: ZW
authors.lastname: Jing
authors.affiliation: Qindu Hospital, Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Ma
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Yi-Yang
authors.initials: YY
authors.lastname: Jia
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Luo
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Xi-Xi
authors.initials: XX
authors.lastname: Ma
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Si-Yuan
authors.initials: SY
authors.lastname: Zhou
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, PR China. Electronic address: blezhang@fmmu.edu.cn.
authors.forename: Bang-Le
authors.initials: BL
authors.lastname: Zhang
mesh_terms: D000447:Aldehydes / Q000737:chemistry*; D000588:Amines / Q000737:chemistry*; D001672:Biocompatible Materials; D018938:Caco-2 Cells; D002240:Carbohydrate Sequence; D048271:Chitosan / Q000737:chemistry*; D004337:Drug Carriers; D006801:Humans; D010084:Oxidation-Reduction; D011092:Polyethylene Glycols / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D012710:Serum Albumin, Bovine / Q000008:administration & dosage*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000447:Aldehydes; D000588:Amines; D001672:Biocompatible Materials; D004337:Drug Carriers; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D048271:Chitosan
keywords: Chitosan; Controlled delivery system; Crosslinking; Hydrogel; Poly(ethylene glycol)