title: Morphologic and Molecular Characterization of a Strain of Zika Virus Imported into Guangdong, China.
abstract: The recent outbreaks of Zika virus (ZIKV) disease have caused worldwide concerns. Guangdong province is one of the commercial centers in China and communicates frequently with the epidemic areas. To date, 65.2% of the ZIKV infection cases in China were imported via port of entry in Guangdong. The continuous surveillance of imported cases is crucial for the prevention and control of potential ZIKV infection outbreak in China. In this study, a strain of ZIKV was isolated from the serum of a 6-year-old child returning from Venezuela. The morphology of the ZIKV was analyzed in vivo and in vitro by electron microscopy, and clusters of virus particles were found in the loose cytoplasmic membrane structures. The genomic sequence of the isolated ZIKV was determined, and the alignment and phylogenetic analysis identified one unique amino acid substitution occurring in the non-structural protein 4B (NS4B), and the isolated virus belonged to the Asian lineage.
authors.affiliation: Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou, P. R. China.|State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, P. R. China.
authors.forename: Shufen
authors.initials: S
authors.lastname: Li
authors.affiliation: Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou, P. R. China.
authors.forename: Yongxia
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou, P. R. China.
authors.forename: Kui
authors.initials: K
authors.lastname: Zheng
authors.affiliation: Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou, P. R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Dai
authors.affiliation: Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou, P. R. China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Li
authors.affiliation: Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou, P. R. China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, P. R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.affiliation: Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou, P. R. China.
authors.forename: Jicheng
authors.initials: J
authors.lastname: Huang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002648:Child; D016679:Genome, Viral; D006801:Humans; D051379:Mice; D008854:Microscopy, Electron; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D012463:Saliva / Q000821:virology; D017386:Sequence Homology, Amino Acid; D014556:Urine / Q000821:virology; D014687:Venezuela / Q000453:epidemiology; D019562:Viral Load; D000071244:Zika Virus / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure*; D000071243:Zika Virus Infection / Q000175:diagnosis* / Q000453:epidemiology / Q000635:transmission*
chemical_list: D012367:RNA, Viral