title: A Semipersistent Plant Virus Differentially Manipulates Feeding Behaviors of Different Sexes and Biotypes of Its Whitefly Vector.
abstract: It is known that plant viruses can change the performance of their vectors. However, there have been no reports on whether or how a semipersistent plant virus manipulates the feeding behaviors of its whitefly vectors. Cucurbit chlorotic yellows virus (CCYV) (genusCrinivirus, familyClosteroviridae) is an emergent plant virus in many Asian countries and is transmitted specifically by B and Q biotypes of tobacco whitefly,Bemisia tabaci(Gennadius), in a semipersistent manner. In the present study, we used electrical penetration graph (EPG) technique to investigate the effect of CCYV on the feeding behaviors ofB. tabaci. The results showed that CCYV altered feeding behaviors of both biotypes and sexes ofB. tabaciwith different degrees. CCYV had stronger effects on feeding behaviors of Q biotype than those of B biotype, by increasing duration of phloem salivation and sap ingestion, and could differentially manipulate feeding behaviors of males and females in both biotype whiteflies, with more phloem ingestion in Q biotype males and more non-phloem probing in B biotype males than their respective females. With regard to feeding behaviors related to virus transmission, these results indicated that, when carrying CCYV,B. tabaciQ biotype plays more roles than B biotype, and males make greater contribution than females.
authors.affiliation: College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China. shaohualu08@163.com.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Lu
authors.affiliation: College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China. lijingjing_319@163.com.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China. wxl2010@yeal.net.
authors.forename: Xueli
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China. songdanyang2@163.com.
authors.forename: Danyang
authors.initials: D
authors.lastname: Song
authors.affiliation: College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China. yxbre@163.com.
authors.forename: Rune
authors.initials: R
authors.lastname: Bai
authors.affiliation: College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China. shiyan00925@126.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 410100, China. guqinsheng@caas.cn.
authors.forename: Qinsheng
authors.initials: Q
authors.lastname: Gu
authors.affiliation: Department of Plant Pathology, University of California, Davis, CA 95616-8600, USA. ywkuo@ucdavis.edu.
authors.forename: Yen-Wen
authors.initials: YW
authors.lastname: Kuo
authors.affiliation: Department of Plant Pathology, University of California, Davis, CA 95616-8600, USA. bwfalk@ucdavis.edu.
authors.forename: Bryce W
authors.initials: BW
authors.lastname: Falk
authors.affiliation: College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China. fmyan@henau.edu.cn.
authors.forename: Fengming
authors.initials: F
authors.lastname: Yan
mesh_terms: D000818:Animals; D029383:Crinivirus / Q000302:isolation & purification*; D005247:Feeding Behavior; D006430:Hemiptera / Q000145:classification / Q000502:physiology* / Q000821:virology*; D012737:Sex Factors
keywords: Bemisia tabaci; CCYV; EPG; cucurbit chlorotic yellows virus; electrical penetration graph; feeding behaviors