title: Expression profile of lncRNA in human bronchial epithelial cells response to Talaromyces marneffei infection: A microarray analysis.
abstract: Talaromyces marneffei is an important opportunistic pathogenic fungus capable of causing systemic lethal infection through inhalation of its conidia. However, little is known about the pathogenesis and interactions between Talaromyces marneffei and host. The aim of this study was to identify potential long noncoding RNAs (lncRNAs) and coding genes associated with interactions between airway epithelial cell and Talaromyces marneffei conidia. We carried out a microarray analysis to determine the expression profile of lncRNA and mRNA in human bronchial epithelial cell in response to Talaromyces marneffei infection. Compared to control group, we found that 370 and 149 lncRNAs were up and down regulated, respectively. Meanwhile, the expression level of 269 and 60 mRNAs was increased and decreased, respectively. To understand the potential role of the differentially expressed lncRNAs, we performed functional annotations of the corresponding coding genes using gene ontology and pathway analyses. Our results provide insights into the pathogenesis of early infection by Talaromyces marneffei.
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
authors.forename: Meihua
authors.initials: M
authors.lastname: Li
authors.affiliation: Guangxi Medical University, Nanning 530021, Guangxi, China.
authors.forename: Honglin
authors.initials: H
authors.lastname: Luo
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Bai
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
authors.forename: Jianquan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Zhong
authors.affiliation: Department of Biochemistry and Molecular Biology, Guangxi Medical University, Nanning 530021, Guangxi, China; Guangxi Colleges and Universities Key Laboratory of Preclinical Medicine Research, Nanning 530021, Guangxi, China.
authors.forename: Xiuwan
authors.initials: X
authors.lastname: Lan
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China; Guangxi Colleges and Universities Key Laboratory of Preclinical Medicine Research, Nanning 530021, Guangxi, China. Electronic address: zhiyi-he@163.com.
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: He
mesh_terms: D002460:Cell Line; D004847:Epithelial Cells / Q000378:metabolism* / Q000382:microbiology*; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions; D006801:Humans; D046228:Microarray Analysis; D062085:RNA, Long Noncoding / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D032901:Talaromyces / Q000254:growth & development*
chemical_list: D062085:RNA, Long Noncoding; D012333:RNA, Messenger
keywords: Fungus; Human bronchial epithelial cell; Microarray analysis; lncRNA