title: Mitochondrial genome of the sweet potato hornworm, Agrius convolvuli (Lepidoptera: Sphingidae), and comparison with other Lepidoptera species.
abstract: In the present study, we sequenced the complete mitochondrial genome (mitogenome) of Agrius convolvuli (Lepidoptera: Sphingidae) and compared it with previously sequenced mitogenomes of lepidopteran species. The mitogenome was a circular molecule, 15 349 base pairs (bp) long, containing 37 genes. The order and orientation of genes in the A. convolvuli mitogenome were similar to those in sequenced mitogenomes of other lepidopterans. All 13 protein-coding genes (PCGs) were initiated by ATN codons, except for the cytochrome c oxidase subunit 1 (cox1) gene, which seemed to be initiated by the codon CGA, as observed in other lepidopterans. Three of the 13 PCGs had the incomplete termination codon T, while the remainder terminated with TAA. Additionally, the codon distributions of the 13 PCGs revealed that Asn, Ile, Leu2, Lys, Phe, and Tyr were the most frequently used codon families. All transfer RNAs were folded into the expected cloverleaf structure except for tRNASer(AGN), which lacked a stable dihydrouridine arm. The length of the adenine (A) + thymine (T)-rich region was 331 bp. This region included the motif ATAGA followed by a 19-bp poly-T stretch and a microsatellite-like (TA)8element next to the motif ATTTA. Phylogenetic analyses (maximum likelihood and Bayesian methods) showed that A. convolvuli belongs to the family Sphingidae.
authors.affiliation: College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, P.R. China.|College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, P.R. China.
authors.forename: Li-Shang
authors.initials: LS
authors.lastname: Dai
authors.affiliation: College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, P.R. China.|College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, P.R. China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, P.R. China.|College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, P.R. China.
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Yu
authors.affiliation: College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, P.R. China.|College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, P.R. China.
authors.forename: Guo-Qing
authors.initials: GQ
authors.lastname: Wei
authors.affiliation: College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, P.R. China.|College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, P.R. China.|College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, P.R. China.
authors.forename: Cen
authors.initials: C
authors.lastname: Qian
authors.affiliation: College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, P.R. China.|College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, P.R. China.
authors.forename: Cong-Fen
authors.initials: CF
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, P.R. China.|College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, P.R. China.|College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.affiliation: College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, P.R. China.|College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, P.R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, P.R. China.|College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, P.R. China.
authors.forename: Bao-Jian
authors.initials: BJ
authors.lastname: Zhu
authors.affiliation: College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, P.R. China.|College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, P.R. China.
authors.forename: Chao-Liang
authors.initials: CL
authors.lastname: Liu
mesh_terms: D000818:Animals; D001482:Base Composition; D019295:Computational Biology / Q000379:methods; D021901:DNA, Intergenic; D023061:Gene Order; D054629:Genome, Mitochondrial; D059014:High-Throughput Nucleotide Sequencing; D027723:Ipomoea batatas / Q000469:parasitology*; D007915:Lepidoptera / Q000145:classification / Q000235:genetics*; D058977:Molecular Sequence Annotation; D016366:Open Reading Frames; D010802:Phylogeny
chemical_list: D021901:DNA, Intergenic
keywords: Agrius convolvuli; Lepidoptera; Sphingidae; génome mitochondrial; mitochondrial genome