title: Screening of enzyme inhibitors from traditional Chinese medicine by magnetic immobilized α-glucosidase coupled with capillary electrophoresis.
abstract: As the close correlation between α-glucosidase inhibitors and the treatment of diabetes, in combination with capillary electrophoresis (CE), a method was developed to screen α-glucosidase inhibitors from traditional Chinese medicines (TCMs) by immobilizing α-glucosidase on magnetic nanoparticles. Such a magnetic immobilization would be beneficial for enzyme reusability, stability and separation. In this work, Fe3O4nanoparticles were synthesized by solvothermal method. And the prepared nanoparticles were characterized by transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), size and zeta potential analysis. With the modification of chitosan (CS) and glutaraldehyde (GA), α-glucosidase was successfully immobilized on the magnetic nanoparticles. The pH and temperature endurance, storage stability and reusability of the immobilized α-glucosidase were studied and compared with those of the free one. With the magnetic immobilized α-glucosidase, the Michaelis-Menten constant (Km) was calculated to be 0.85mM, and the inhibition constant (Ki) and half-maximal inhibitory concentration (IC50) for acarbose were determined to be 7.37 and 13.69μM, respectively. Finally, the developed method was applied to screen α-glucosidase inhibitors from 18 TCMs.
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, People's Republic of China; University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Liu
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, People's Republic of China. Electronic address: chenjuan@licp.cas.cn.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, People's Republic of China. Electronic address: shiyp@licp.cas.cn.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Shi
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D048271:Chitosan / Q000737:chemistry; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D004800:Enzymes, Immobilized / Q000037:antagonists & inhibitors / Q000737:chemistry; D052203:Ferrosoferric Oxide / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D065089:Glycoside Hydrolase Inhibitors / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D059346:Magnets / Q000737:chemistry*; D008516:Medicine, Chinese Traditional; D013696:Temperature; D000520:alpha-Glucosidases / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D004800:Enzymes, Immobilized; D065089:Glycoside Hydrolase Inhibitors; D048271:Chitosan; D000520:alpha-Glucosidases; D005976:Glutaral; D052203:Ferrosoferric Oxide
keywords: Immobilized α-glucosidase; Inhibitor screening; Magnetic nanoparticles; Traditional Chinese medicine