title: Towards new sources of resistance to the currant-lettuce aphid (Nasonovia ribisnigri).
abstract: Domesticated lettuce varieties encompass much morphological variation across a range of crop type groups, with large collections of cultivars and landrace accessions maintained in genebanks. Additional variation not captured during domestication, present in ancestral wild relatives, represents a potentially rich source of alleles that can deliver to sustainable crop production. However, these large collections are difficult and costly to screen for many agronomically important traits. In this paper, we describe the generation of a diversity collection of 96 lettuce and wild species accessions that are amenable to routine phenotypic analysis and their genotypic characterization with a panel of 682 newly developed expressed sequence tag (EST)-linked KASP™ single nucleotide polymorphism (SNP) markers that are anchored to the draftLactuca sativagenome assembly. To exemplify the utility of these resources, we screened the collection for putative sources of resistance to currant-lettuce aphid (Nasonovia ribisnigri) and carried out association analyses to look for potential SNPs linked to resistance.
authors.affiliation: Functional and Comparative Genomics, Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZB UK.
authors.forename: Peter G
authors.initials: PG
authors.lastname: Walley
authors.affiliation: Syngenta, Jealott's Hill International Research Centre, Bracknell, Berkshire RG42 6EY UK.
authors.forename: Gemma
authors.initials: G
authors.lastname: Hough
authors.affiliation: Earlham Institute, Norwich Research Park, Norwich, NR4 7UH UK.
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Moore
authors.affiliation: School of Life Sciences, Warwick Crop Centre, The University of Warwick, Wellesbourne, Warwick, CV35 9EF UK.
authors.forename: John
authors.initials: J
authors.lastname: Carder
authors.affiliation: School of Life Sciences, Warwick Crop Centre, The University of Warwick, Wellesbourne, Warwick, CV35 9EF UK.
authors.forename: Marian
authors.initials: M
authors.lastname: Elliott
authors.affiliation: Rothamsted Research, West Common, Harpenden, Hertfordshire AL5 2JQ UK.
authors.forename: Andrew
authors.initials: A
authors.lastname: Mead
authors.affiliation: School of Life Sciences, Warwick Crop Centre, The University of Warwick, Wellesbourne, Warwick, CV35 9EF UK.
authors.forename: Julie
authors.initials: J
authors.lastname: Jones
authors.affiliation: School of Life Sciences, Warwick Crop Centre, The University of Warwick, Wellesbourne, Warwick, CV35 9EF UK.
authors.forename: Graham
authors.initials: G
authors.lastname: Teakle
authors.affiliation: School of Life Sciences, Warwick Crop Centre, The University of Warwick, Wellesbourne, Warwick, CV35 9EF UK.
authors.forename: Guy
authors.initials: G
authors.lastname: Barker
authors.affiliation: School of Life Sciences, Warwick Crop Centre, The University of Warwick, Wellesbourne, Warwick, CV35 9EF UK.
authors.forename: Vicky
authors.initials: V
authors.lastname: Buchanan-Wollaston
authors.affiliation: Harper Adams University College, Newport, Shropshire TF10 8NB UK.
authors.forename: Paul
authors.initials: P
authors.lastname: Hand
authors.affiliation: Harper Adams University College, Newport, Shropshire TF10 8NB UK.
authors.forename: David
authors.initials: D
authors.lastname: Pink
authors.affiliation: School of Life Sciences, Warwick Crop Centre, The University of Warwick, Wellesbourne, Warwick, CV35 9EF UK.
authors.forename: Rosemary
authors.initials: R
authors.lastname: Collier
keywords: Aphid; Association; Diversity; Lettuce; Resistance; SNP