title: α-l-rhamnosidase selective for rutin to isoquercitrin transformation from Penicillium griseoroseum MTCC-9224.
abstract: An α-l-rhamnosidase secreting fungal strain has been isolated from the decaying goose berry (Emblica officinalis) fruit peel. The fungal strain has been identified as Penicillium greoroseum MTCC-9224. The α-l-rhamnosidase of this fungal strain has been purified to homogeneity using a simple procedure involving concentration by ultra filtration and an anion exchange chromatography on DEAE-cellulose. The purified enzyme gave a single protein band corresponding to molecular mass of 97kDa in SDS-PAGE analysis. The native-PAGE analysis also gave a single protein band confirming the purity of the enzyme. Using p-nitrophenyl-α-l-rhamnopyranoside as the substrate, Kmand kcatvalues of the enzyme were 0.65mM and 43.65s-1, respectively. The pH and temperature optima of the enzyme were 6.5 and 57°C, respectively. The activation energy for the thermal denaturation of the enzyme was 27.9kJ/mol. The purified α-l-rhamnosidase hydrolyzed rutin to isoquercitrin and l-rhamnose but has no effect on naringin and hesperidin.
authors.affiliation: Department of Chemistry, DDU Gorakhpur University Gorakhpur, Gorakhpur 273009, Uttar Pradesh, India. Electronic address: dr_saritayadav@rediffmail.com.
authors.forename: Sarita
authors.initials: S
authors.lastname: Yadav
authors.affiliation: Department of Chemistry, DDU Gorakhpur University Gorakhpur, Gorakhpur 273009, Uttar Pradesh, India.
authors.forename: Sudha
authors.initials: S
authors.lastname: Yadava
authors.affiliation: Department of Chemistry, DDU Gorakhpur University Gorakhpur, Gorakhpur 273009, Uttar Pradesh, India.
authors.forename: Kapil D S
authors.initials: KD
authors.lastname: Yadav
mesh_terms: D005638:Fruit / Q000382:microbiology; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D010407:Penicillium / Q000737:chemistry / Q000201:enzymology* / Q000302:isolation & purification / Q000378:metabolism; D029842:Phyllanthus emblica / Q000382:microbiology; D011489:Protein Denaturation; D011794:Quercetin / Q000031:analogs & derivatives* / Q000378:metabolism; D012431:Rutin / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature; D013816:Thermodynamics
chemical_list: C016527:isoquercitrin; D012431:Rutin; D011794:Quercetin; D006026:Glycoside Hydrolases; C022525:alpha-L-rhamnosidase
keywords: Emblica officinalis; Glycoside hydrolase; Isoquercitrin; Penicillium griseoroseum; Rutin