title: Evaluation of five serologic assays for bovine tuberculosis surveillance in domestic free-range pigs from southern Spain.
abstract: In countries where bovine tuberculosis (bTB) is still prevalent the contact among different animal species in extensive systems contributes to the circulation of Mycobacterium bovis (M. bovis) and other members of the Mycobacterium tuberculosis complex (MTC). Thus, free-range pigs can develop subclinical infections and may contribute to disease spread to bovine and wildlife. Serodiagnosis has been proposed as a screening tool for detecting infected pig herds; however, the value of this method to obtain an accurate diagnosis in this species is still not clear. In this study, sensitivity (Se) and specificity (Sp) estimates of four ELISAs and a lateral flow immunochromatographic antibody assay based on different M. bovis antigens, including MPB70 and MPB83 proteins, were evaluated in naturally infected domestic free-range pigs. For this purpose, submandibular lymph nodes and blood samples from 217 pigs from both TB-infected and historically negative farms were sampled at slaughterhouse and analysed by gross examination, histopathology, bacteriological culture and qPCR. Se and Sp estimates of the 5 evaluated assays ranged from 66.1% to 78% (CI95from 52.6 to 87.7%) and from 98.9% to 100% (CI95from 93.8 to 100%), respectively. Results of our study suggest that all the evaluated assays could be used as a first screening tool to conduct bTB surveillance in domestic pigs at population level; however, animals from seropositive herds should later be surveyed by other methods in order to reduce false negative results.
authors.affiliation: CICAP-Agrifood Research Centre, Pozoblanco, 14400 Córdoba, Spain; Animal Health Department, University of Córdoba, International Excellence Agrifood Campus 'CeiA3', 14071 Córdoba, Spain. Electronic address: fcardoso@cicap.es.
authors.forename: Fernando
authors.initials: F
authors.lastname: Cardoso-Toset
authors.affiliation: Animal Health Department, University of Córdoba, International Excellence Agrifood Campus 'CeiA3', 14071 Córdoba, Spain.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Luque
authors.affiliation: Department of Anatomy and Comparative Pathology, University of Córdoba, International Excellence Agrifood Campus 'CeiA3', 14071 Córdoba, Spain.
authors.forename: Librado
authors.initials: L
authors.lastname: Carrasco
authors.affiliation: CICAP-Agrifood Research Centre, Pozoblanco, 14400 Córdoba, Spain; Animal Health Department, University of Córdoba, International Excellence Agrifood Campus 'CeiA3', 14071 Córdoba, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Jurado-Martos
authors.affiliation: Instituto de Investigación en Recursos Cinegéticos, IREC, (CSIC-UCLM-JCCM), Ronda de Toledo s/n, 13071 Ciudad Real, Spain.
authors.forename: María Ángeles
authors.initials: MÁ
authors.lastname: Risalde
authors.affiliation: Inmunología y Genética Aplicada, S.A. (INGENASA), Calle Hermanos García Noblejas 39, 28037 Madrid, Spain.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Venteo
authors.affiliation: VISAVET Health Surveillance Centre, Faculty of Veterinary Medicine, Complutense University, 28040 Madrid, Spain; Servicio de Inmunología Microbiana, Centro Nacional de Microbiología, Instituto de Investigación Carlos III, 28220 Madrid, Spain.
authors.forename: José A
authors.initials: JA
authors.lastname: Infantes-Lorenzo
authors.affiliation: VISAVET Health Surveillance Centre, Faculty of Veterinary Medicine, Complutense University, 28040 Madrid, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Bezos
authors.affiliation: Inmunología y Genética Aplicada, S.A. (INGENASA), Calle Hermanos García Noblejas 39, 28037 Madrid, Spain.
authors.forename: Paloma
authors.initials: P
authors.lastname: Rueda
authors.affiliation: Inmunología y Genética Aplicada, S.A. (INGENASA), Calle Hermanos García Noblejas 39, 28037 Madrid, Spain.
authors.forename: Istar
authors.initials: I
authors.lastname: Tapia
authors.affiliation: Instituto de Investigación en Recursos Cinegéticos, IREC, (CSIC-UCLM-JCCM), Ronda de Toledo s/n, 13071 Ciudad Real, Spain.
authors.forename: Christian
authors.initials: C
authors.lastname: Gortázar
authors.affiliation: VISAVET Health Surveillance Centre, Faculty of Veterinary Medicine, Complutense University, 28040 Madrid, Spain.
authors.forename: Lucas
authors.initials: L
authors.lastname: Domínguez
authors.affiliation: Servicio de Inmunología Microbiana, Centro Nacional de Microbiología, Instituto de Investigación Carlos III, 28220 Madrid, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Domínguez
authors.affiliation: CICAP-Agrifood Research Centre, Pozoblanco, 14400 Córdoba, Spain; Department of Anatomy and Comparative Pathology, University of Córdoba, International Excellence Agrifood Campus 'CeiA3', 14071 Córdoba, Spain.
authors.forename: Jaime
authors.initials: J
authors.lastname: Gomez-Laguna
mesh_terms: D000818:Animals; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D011159:Population Surveillance / Q000379:methods; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods / Q000662:veterinary; D013030:Spain; D013552:Swine / Q000382:microbiology; D013553:Swine Diseases / Q000175:diagnosis* / Q000382:microbiology; D014380:Tuberculosis, Bovine / Q000175:diagnosis*
keywords: MPB83; MTC; Pigs; Serologic assays; Tuberculosis-like lesions; bPPD