title: A simple 2D composite image analysis technique for the crystal growth study of L-ascorbic acid.
abstract: This work was destined for 2D crystal growth studies of L-ascorbic acid using the composite image analysis technique. Growth experiments on the L-ascorbic acid crystals were carried out by standard (optical) microscopy, laser diffraction analysis, and composite image analysis. For image analysis, the growth of L-ascorbic acid crystals was captured as digital 2D RGB images, which were then processed to composite images. After processing, the crystal boundaries emerged as white lines against the black (cancelled) background. The crystal boundaries were well differentiated by peaks in the intensity graphs generated for the composite images. The lengths of crystal boundaries measured from the intensity graphs of composite images were in good agreement (correlation coefficient "r" = 0.99) with the lengths measured by standard microscopy. On the contrary, the lengths measured by laser diffraction were poorly correlated with both techniques. Therefore, the composite image analysis can replace the standard microscopy technique for the crystal growth studies of L-ascorbic acid.
authors.affiliation: College of Pharmacy, Pt. B.D. Sharma University of Health Sciences, Rohtak, 124001, Haryana, India.|M&E, Home Hygiene surface and Personal care, Reckitt Benckiser India Pvt. Ltd, Gurgaon, 122001, Haryana, India.
authors.forename: Krishan
authors.initials: K
authors.lastname: Kumar
authors.affiliation: College of Pharmacy, Pt. B.D. Sharma University of Health Sciences, Rohtak, 124001, Haryana, India.
authors.forename: Virender
authors.initials: V
authors.lastname: Kumar
authors.affiliation: PGIMS, Pt. B.D. Sharma University of Health Sciences, Rohtak, 124001, Haryana, India.
authors.forename: Jatin
authors.initials: J
authors.lastname: Lal
authors.affiliation: Department of Pharmaceutical Sciences, M.D. University, Rohtak, 124001, Haryana, India.
authors.forename: Harmeet
authors.initials: H
authors.lastname: Kaur
authors.affiliation: College of Pharmacy, Pt. B.D. Sharma University of Health Sciences, Rohtak, 124001, Haryana, India.
authors.forename: Jasbir
authors.identifier: http://orcid.org/0000-0001-8549-6233
authors.initials: J
authors.lastname: Singh
keywords: analysis; crystal; images; microscopy; pharmaceuticals