title: Anti-hyperuricemic effect of taxifolin in cultured hepatocytes and model mice.
abstract: Hyperuricemia is recognized as an important risk factor for gout. High dietary intake of purine-rich foods such as meats and sea foods increases uric acid (UA) levels in the blood. Taxifolin present in Siberian larch and strawberries has been reported to possess health promoting activities including anti-oxidant effect. In this study, we examined anti-hyperuricemic effect of taxifolin in both cultured hepatocytes and hyperuricemic model mice. In cultured AML12 hepatocytes, taxifolin significantly suppressed UA production dose- and time-dependently. In mice with hyperuricemia induced by concurrent administration of guanosine-5'-monophosphate and inosine-5'-monophosphate, oral administration of taxifolin suppressed the increases in plasma and liver UA levels. In addition, it also suppressed hepatic xanthine oxidase (XO) activity. Thus, anti-hyperuricemic effect of taxifolin could be explained, at least partly, by suppressing UA production via inhibition of XO activity in the liver. These results suggest that taxifolin possesses a potent hypouricemic effect and it could be a potential candidate for an anti-hyperuricemic phytochemical.
authors.affiliation: Center for Bioscience Research and Education, Utsunomiya University, Tochigi, 321-8505, Japan.
authors.forename: Shin-Ichi
authors.initials: SI
authors.lastname: Adachi
authors.affiliation: Faculty of Agriculture, Utsunomiya University, Tochigi, 321-8505, Japan.|Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo, 183-8509, Japan.
authors.forename: Ken-Ichi
authors.initials: KI
authors.lastname: Nihei
authors.affiliation: Strawberry Research Center, Tochigi Prefectural Agricultural Experiment Station, Tochigi, 328-0007, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Ishihara
authors.affiliation: Faculty of Agriculture, Utsunomiya University, Tochigi, 321-8505, Japan.|Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo, 183-8509, Japan.
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Yoshizawa
authors.affiliation: Center for Bioscience Research and Education, Utsunomiya University, Tochigi, 321-8505, Japan. yagasaki@cc.tuat.ac.jp.
authors.forename: Kazumi
authors.identifier: http://orcid.org/0000-0002-9494-9887
authors.initials: K
authors.lastname: Yagasaki
keywords: AML12 hepatocyte; Hyperuricemia; Taxifolin; Uric acid