title: Phenotypic and biochemical alterations in relation to MT2 gene expression in Plantago ovata Forsk under zinc stress.
abstract: Plantago ovata Forsk is an annual herb with immense medicinal importance, the seed and husk of which is used in the treatment of chronic constipation, irritable bowel syndrome, diarrhea since ancient times. Zinc, an essential metal, is required by plants as they form important components of zinc finger proteins and also aid in synthesis of photosynthetic pigments such as chlorophyll. However, in excess amount Zn causes chlorosis of leaf and shoot tissues and generate reactive oxygen species. The present study is aimed at investigating the changes in expression levels of MT2 gene in Plantago ovata under zinc stress. Data show up to 1.66 fold increase in expression of PoMT2 in 1000 µM ZnSO4·7H2O treated sample. Our study also describes alteration of MT2 gene expressions in Plantago ovata as observed through Real time PCR (qPCR) done by [Formula: see text] method. In this study we have observed an upregulation (or induction) in the PoMT2 gene expression level in 500 and 800 µM ZnSO4·7H2O treated samples but found saturation on further increasing the dose to 1000 µM of ZnSO4·7H2O. Determination of the phenotypic and biochemical changes in Plantago ovata due to exposure to zinc stress of concentrations 500, 800 and 1000 µM revealed oxidative stress. The enhanced expression of MT2 gene in Plantago ovata has a correlation with the increased total antioxidant activity and increased DPPH radical scavenging activity.
authors.affiliation: Department of Biophysics, Molecular Biology and Bioinformatics, University of Calcutta, 92, APC Road, Kolkata, 700009, India.
authors.forename: Paulami
authors.initials: P
authors.lastname: Pramanick
authors.affiliation: Kolkata Centre, UGC- DAE Consortium for Scientific Research, LB- 8, Sector- III, Bidhan Nagar, Kolkata, 700098, India.
authors.forename: Anindita
authors.initials: A
authors.lastname: Chakraborty
authors.affiliation: Department of Biophysics, Molecular Biology and Bioinformatics, University of Calcutta, 92, APC Road, Kolkata, 700009, India. sarmistharc@gmail.com.
authors.forename: Sarmistha Sen
authors.initials: SS
authors.lastname: Raychaudhuri
mesh_terms: D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors / Q000737:chemistry; D002734:Chlorophyll / Q000096:biosynthesis; D000077194:Chlorophyll A; D004305:Dose-Response Relationship, Drug; D018506:Gene Expression Regulation, Plant; D008668:Metallothionein / Q000819:agonists / Q000096:biosynthesis / Q000235:genetics*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010851:Picrates / Q000037:antagonists & inhibitors / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D010940:Plant Proteins / Q000819:agonists / Q000096:biosynthesis / Q000235:genetics*; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D010943:Plantago / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D019287:Zinc Sulfate / Q000633:toxicity*
chemical_list: D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; D010940:Plant Proteins; D002734:Chlorophyll; D019287:Zinc Sulfate; D008668:Metallothionein; C004931:1,1-diphenyl-2-picrylhydrazyl; D000077194:Chlorophyll A
keywords: Antioxidant; Metallothionein; Oxidative stress; Plantago ovata; Zinc