title: ADAR1 overexpression is associated with cervical cancer progression and angiogenesis.
abstract: BACKGROUND
  This study aimed to assess the role of RNA-dependent adenosine deaminase (ADAR1) in cervical squamous cell carcinoma occurrence and progression.
  
  
  METHODS
  ADAR1 expression levels in stage IA and stage IIA cervical squamous cell carcinoma (group A), cervical intraepithelial neoplasia (CIN) specimens (group B), as well as normal and inflamed cervical tissue samples (group C) were assessed by immunohistochemistry. Clinical and pathological data of cervical squamous cell carcinoma patients undergoing surgery were retrospectively evaluated. Chi-square test, comparative analysis of survival curve, disease-free survival and COX risk assessment method were used to understand the association of ADAR1 with the occurrence and progression and prognostic significance of cervical squamous cell carcinoma.
  
  
  RESULTS
  ADAR1 is expressed in the cytoplasm and nuclei. The expression level was high in squamous cell carcinoma tissues (81.18%), while relatively low in the CIN group (21.56%). And there was no expression in non-cancerous tissues. The differences between them were statistically significant using P < 0.05 as criterion. One-factor analysis revealed that ADAR1 was significantly correlated with tumor diameter, horizontal diffusion diameter, vascular invasion, parametrial invasion, vaginal involvement, and pathologically diagnostic criteria for perineural invasion (PNI). Meanwhile, the overall survival rate of ADAR1 positive patients was significantly lower compared with that of patients with no ADAR1 expression (P < 0.05). Analysis also showed that disease-free survival time of ADAR1 positive patients was shorter than that of ADAR1 negative patients, and the difference was significant (P < 0.01). Finally, COX risk assessment showed that parametrical invasion had independent prognostic factors for overall survival of squamous cell carcinoma.
  
  
  CONCLUSIONS
  Results indicated that ADAR1 might play an important role in the occurrence, progression and prognosis of cervical squamous cancer.
authors.affiliation: Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.|Department of Obstetrics and Gynecology, Sichuan Province, Chengdu Women's and Children's Central Hospital, Chengdu, 610091, Sichuan, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, 610041, Sichuan, China. Wanghe_cd@126.com.
authors.forename: He
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Pathology, Sichuan Province, Chengdu Women's and Children's Central Hospital, Chengdu, 610091, China.
authors.forename: Wenyi
authors.initials: W
authors.lastname: Lin
authors.affiliation: Department Physical Examination, Sichuan People's Hospital, Chengdu, 610072, Sichuan, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Shuai
mesh_terms: D000243:Adenosine Deaminase / Q000032:analysis / Q000096:biosynthesis*; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000401:mortality / Q000473:pathology*; D018450:Disease Progression; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000473:pathology*; D011379:Prognosis; D016016:Proportional Hazards Models; D016601:RNA-Binding Proteins / Q000032:analysis / Q000096:biosynthesis*; D012189:Retrospective Studies; D015854:Up-Regulation; D002583:Uterine Cervical Neoplasms / Q000098:blood supply / Q000401:mortality / Q000473:pathology*; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D016601:RNA-Binding Proteins; C000595356:ADAR protein, human; D000243:Adenosine Deaminase
keywords: Adenosine deaminase acting on RNA1; Cervical squamous cancer; Risk factor