title: Occurrence of complex type free N-glycans with a single GlcNAc residue at the reducing termini in the fresh-water plant, Egeria densa.
abstract: In our previous study, we found unique free N-glycans (FNGs), which carry a single GlcNAc residue (GN1) at the reducing-end side and the Lewis-a epitope at the non-reducing-end side, in the culture broth of rice cells. Based on the FNG structural features and the substrate specificity of plant ENGase, we hypothesized that there might be a novel biosynthetic mechanism responsible for the production of these unique GN1-FNGs, in which high-mannose type (HMT)-GN1-FNGs produced in the cytosol from misfolded glycoproteins by ENGase are transported back into the endoplasmic reticulum and processed to plant complex type (PCT)-GN1-FNGs in the Golgi apparatus. Until now, however, PCT-GN1-FNGs had only been found in the culture broth of rice cultured cells and never in plants, suggesting that the formation of PCT-GN1-FNGs might be generated under special or artificial conditions. In this study, we confirm the presence of PCT-GN1-FNGs, HMT-GN1-FNGs and PCT-GN2-FNGs in the fresh-water plant Egeria densa. These results suggest that a mechanism responsible for the production of PCT-GN1-FNG is present in native plant tissues.
authors.affiliation: Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530, Japan.|Faculty of Agriculture, Division of Agricultural Science, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Maeda
authors.affiliation: Faculty of Agriculture, Division of Agricultural Science, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530, Japan.
authors.forename: Natsuki
authors.initials: N
authors.lastname: Ebara
authors.affiliation: Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530, Japan.
authors.forename: Misato
authors.initials: M
authors.lastname: Tani
authors.affiliation: Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530, Japan.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Vavricka
authors.affiliation: Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530, Japan. yosh8mar@okayama-u.ac.jp.|Faculty of Agriculture, Division of Agricultural Science, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530, Japan. yosh8mar@okayama-u.ac.jp.
authors.forename: Yoshinobu
authors.identifier: 0000-0003-0329-1168
authors.initials: Y
authors.lastname: Kimura
mesh_terms: D000117:Acetylglucosamine / Q000737:chemistry / Q000378:metabolism*; D002236:Carbohydrate Conformation; D019684:Magnoliopsida / Q000737:chemistry / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*
chemical_list: D011134:Polysaccharides; D000117:Acetylglucosamine
keywords: Deglycosylation; Egeria densa; Free N-glycans; Glycoprotein metabolism; Plant N-glycans