title: Expression Profile of Long Noncoding RNAs in Human Earlobe Keloids: A Microarray Analysis.
abstract: Background. Long noncoding RNAs (lncRNAs) play key roles in a wide range of biological processes and their deregulation results in human disease, including keloids. Earlobe keloid is a type of pathological skin scar, and the molecular pathogenesis of this disease remains largely unknown.Methods. In this study, microarray analysis was used to determine the expression profiles of lncRNAs and mRNAs between 3 pairs of earlobe keloid and normal specimens. Gene Ontology (GO) categories and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to identify the main functions of the differentially expressed genes and earlobe keloid-related pathways.Results. A total of 2068 lncRNAs and 1511 mRNAs were differentially expressed between earlobe keloid and normal tissues. Among them, 1290 lncRNAs and 1092 mRNAs were upregulated, and 778 lncRNAs and 419 mRNAs were downregulated. Pathway analysis revealed that 24 pathways were correlated to the upregulated transcripts, while 11 pathways were associated with the downregulated transcripts.Conclusion. We characterized the expression profiles of lncRNA and mRNA in earlobe keloids and suggest that lncRNAs may serve as diagnostic biomarkers for the therapy of earlobe keloid.
authors.affiliation: Department of Plastic Surgery, Wuhan General Hospital of Guangzhou Military Command of Chinese PLA, Wuhan, Hubei, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Guo
authors.affiliation: Department of Plastic Surgery, Wuhan General Hospital of Guangzhou Military Command of Chinese PLA, Wuhan, Hubei, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Xu
authors.affiliation: Department of Plastic Surgery, Wuhan General Hospital of Guangzhou Military Command of Chinese PLA, Wuhan, Hubei, China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Yan
authors.affiliation: Department of Plastic Surgery, Wuhan General Hospital of Guangzhou Military Command of Chinese PLA, Wuhan, Hubei, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Feng
authors.affiliation: Department of Plastic and Reconstructive Surgery, Southwestern Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Linlin
authors.identifier: 0000-0003-3568-4046
authors.initials: L
authors.lastname: Chai
authors.affiliation: Department of Neurosurgery, Wuhan General Hospital of Guangzhou Military Command of Chinese PLA, Wuhan, Hubei, China.
authors.forename: Guozheng
authors.identifier: 0000-0002-1752-2089
authors.initials: G
authors.lastname: Xu
mesh_terms: D000328:Adult; D004431:Ear, External / Q000378:metabolism* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D006801:Humans; D007627:Keloid / Q000378:metabolism* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D062085:RNA, Long Noncoding / Q000096:biosynthesis*
chemical_list: D062085:RNA, Long Noncoding