title: Long-term prevalence of the protists Crithidia bombi and Apicystis bombi and detection of the microsporidium Nosema bombi in invasive bumble bees.
abstract: An initial survey in 2009 carried out at a site in northwestern Patagonia region, Argentina, revealed for the first time in South America the presence of the flagellate Crithidia bombi and the neogregarine Apicystis bombi, two pathogens associated with the Palaearctic invasive bumble bee Bombus terrestris. In order to determine the long-term persistence and dynamics of this microparasite complex, four additional collections at the same site (San Carlos de Bariloche) were conducted along the following seven years. Both protists were detected in all collections: prevalence was 2%-21.6% for C. bombi and 1.2%-14% for A. bombi. In addition, the microsporidium Nosema bombi was recorded for the first time in the country in the last two collections, at prevalences of 12.4% and 2.4% and unusually high infection intensities (Average = 6.56 × 107spores per individual). Due to the exceptional dispersal ability of the exotic B. terrestris, these three multihost pathogens should be considered as potential threats to South American native bumble bees.
authors.affiliation: Centro de Estudios Parasitológicos y de Vectores (CONICET - UNLP), La Plata, Argentina.
authors.forename: Santiago
authors.initials: S
authors.lastname: Plischuk
authors.affiliation: Instituto de Investigaciones Biológicas "Clemente Estable,", Montevideo, Uruguay.
authors.forename: Karina
authors.initials: K
authors.lastname: Antúnez
authors.affiliation: Centro de Estudios Parasitológicos y de Vectores (CONICET - UNLP), La Plata, Argentina.
authors.forename: Marina
authors.initials: M
authors.lastname: Haramboure
authors.affiliation: Centro de Estudios Parasitológicos y de Vectores (CONICET - UNLP), La Plata, Argentina.
authors.forename: Graciela M
authors.initials: GM
authors.lastname: Minardi
authors.affiliation: Centro de Estudios Parasitológicos y de Vectores (CONICET - UNLP), La Plata, Argentina.|Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CICPBA), Buenos Aires, Argentina.
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Lange
mesh_terms: D000818:Animals; D016782:Apicomplexa / Q000302:isolation & purification*; D001118:Argentina; D001516:Bees / Q000382:microbiology* / Q000469:parasitology*; D003421:Crithidia / Q000302:isolation & purification*; D016818:Nosema / Q000302:isolation & purification*; D015995:Prevalence