title: The involvement of MsmK in pathogenesis of the Streptococcus suis serotype 2.
abstract: Streptococcus suis serotype 2 (SS2) is an important swine and human pathogen that causes global economic and public health problems. Virulent S. suis strains successfully maintain high bacterial concentrations in host blood and rapidly adapt to challenging environments within hosts. Successful survival in hosts is a major factor influencing the pathogenesis of SS2. We have previously identified that SS2 colonization in mouse brain is possibly affected by the ATPase, MsmK of carbohydrate ATP-binding cassette (ABC) transporters because of carbohydrate utilization. In this study, the chain length of the msmK deletion mutant was longer than that of the wild type, and the former was significantly more susceptible than the latter when theses strains were exposed to mouse blood both in vivo and in vitro. The hemolytic activity of the mutant strain was decreased. Although the adhesion of the mutant to HEp-2 cell lines was enhanced, the deletion of msmK impaired the abilities of SS2 to resist phagocytosis and survive severe stress conditions. MsmK contributed to the survival and adaptation of SS2 in host bloodstream. Therefore, MsmK was identified as a multifunctional component that not only contributed to carbohydrate utilization but also participated in SS2 pathogenesis.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.|Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang, China.
authors.forename: Mei-Fang
authors.initials: MF
authors.lastname: Tan
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
authors.forename: Wan-Quan
authors.initials: WQ
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Zhang
authors.affiliation: Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
authors.forename: De-Xin
authors.initials: DX
authors.lastname: Qiu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.|Cooperative Innovation Center of Sustainable Pig Production, Wuhan, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Li
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.|Cooperative Innovation Center of Sustainable Pig Production, Wuhan, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhou
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000251:Adenosine Triphosphatases / Q000235:genetics*; D000818:Animals; D016470:Bacteremia / Q000382:microbiology; D001422:Bacterial Adhesion / Q000235:genetics; D050260:Carbohydrate Metabolism / Q000235:genetics*; D002460:Cell Line; D005260:Female; D017353:Gene Deletion; D006801:Humans; D051379:Mice; D018384:Oxidative Stress / Q000235:genetics; D010587:Phagocytosis; D013290:Streptococcal Infections / Q000382:microbiology / Q000473:pathology*; D017011:Streptococcus suis / Q000378:metabolism* / Q000472:pathogenicity*
chemical_list: D018528:ATP-Binding Cassette Transporters; D000251:Adenosine Triphosphatases
keywords: 
  Streptococcus suis
  ; MsmK; bloodstream; pathogenesis