title: Synthetic Assembly of Mannose Moieties Using Polymer Chemistry and the Biological Evaluation of Its Interaction towards Concanavalin A.
abstract: Protein-carbohydrate interactions exhibit myriad intracellular recognition events, so understanding and investigating their specific interaction with high selectivity and strength are of crucial importance. In order to examine the effect of multivalent binding on the specificity of protein-carbohydrate interactions, we synthesized mannose glycosides as a novel type of glycosylated monomer and glycopolymers of polyacrylamide derivatives with α-mannose (α-Man) by radical polymerization and monitored their strength of interaction with concanavalin A (Con A) by surface plasmon resonance (SPR) detection. In a quantitative test using the Con A-immobilized sensor surface, the kinetic affinity for the synthesized polymers,8a(KD= 3.3 × 10-6M) and8b(KD= 5.3 × 10-5M), were concentration-dependent, showing strong, specific molecular recognition abilities with lectin. Our study showed the enhancement in recognition specificity for multivalent saccharides, which is often mediated by cell surface carbohydrate-binding proteins that exhibit weak affinity and broad specificity for the individual ligands.
authors.affiliation: Division of Material Science, Graduate School of Science and Engineering, Saitama University, Sakura, Saitama 338-8570, Japan. deepti992@gmail.com.
authors.forename: Deepti
authors.initials: D
authors.lastname: Diwan
authors.affiliation: Division of Material Science, Graduate School of Science and Engineering, Saitama University, Sakura, Saitama 338-8570, Japan. k.shinkai917@gmail.com.
authors.forename: Kohei
authors.initials: K
authors.lastname: Shinkai
authors.affiliation: Division of Material Science, Graduate School of Science and Engineering, Saitama University, Sakura, Saitama 338-8570, Japan. t.tetsuka.0924@gmail.com.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Tetsuka
authors.affiliation: Division of Material Science, Graduate School of Science and Engineering, Saitama University, Sakura, Saitama 338-8570, Japan. bin.cao@colorado.edu.
authors.forename: Bin
authors.initials: B
authors.lastname: Cao
authors.affiliation: Division of Material Science, Graduate School of Science and Engineering, Saitama University, Sakura, Saitama 338-8570, Japan. harai@mail.saitama-u.ac.jp.
authors.forename: Hidenao
authors.initials: H
authors.lastname: Arai
authors.affiliation: Division of Material Science, Graduate School of Science and Engineering, Saitama University, Sakura, Saitama 338-8570, Japan. koyama@fms.saitama-u.ac.jp.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Koyama
authors.affiliation: Division of Material Science, Graduate School of Science and Engineering, Saitama University, Sakura, Saitama 338-8570, Japan. khatano@fms.saitama-u.ac.jp.
authors.forename: Ken
authors.initials: K
authors.lastname: Hatano
authors.affiliation: Division of Material Science, Graduate School of Science and Engineering, Saitama University, Sakura, Saitama 338-8570, Japan. koji@fms.saitama-u.ac.jp.
authors.forename: Koji
authors.initials: K
authors.lastname: Matsuoka
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D003208:Concanavalin A / Q000737:chemistry*; D005609:Free Radicals / Q000737:chemistry; D007700:Kinetics; D008358:Mannose / Q000737:chemistry*; D008362:Mannosides / Q000138:chemical synthesis*; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis*; D011485:Protein Binding; D020349:Surface Plasmon Resonance; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D003208:Concanavalin A; D005609:Free Radicals; D008358:Mannose; D008362:Mannosides; D011108:Polymers; D000099166:Polyacrylamides
keywords: concanavalin A; glycopolymers; kinetic affinity; mannose; radical polymerization; surface plasmon resonance