title: Molecular Detection ofAnaplasmaspp. andEhrlichiaspp. in Ruminants from Twelve Provinces of China.
abstract: Anaplasmaspp. andEhrlichiaspp. are tick-transmitted bacteria that are of significant economic importance as they can infect large and small ruminants and also people. There is little information on anaplasmosis and ehrlichiosis in ruminants in China. 16S rRNA FRET-qPCRs were used to screen convenience whole blood samples from 2,240 domestic ruminants in 12 provinces of China forAnaplasmaspp. andEhrlichiaspp. Positive samples were further analyzed with a standard PCR for thegltA.Anaplasmaspp. DNA was detected in the sheep (11.7%; 13/111), goats (81.8%; 219/270), cattle (13.2%; 241/1,830), and water buffaloes (6.9%; 2/29).Ehrlichiaspp. DNA was detected in sheep (1.8%; 2/111), goats (1.1%; 3/270), and cattle (3.6%; 65/1830) but not in water buffaloes (0/29). Sequencing ofgltAPCR products showed thatA. marginale,A. ovis,Ehrlichia canis, andEhrlichiasp. (JX629807) were present in ruminants from China, while the 16S rRNA FRET-qPCR sequence data indicated that there might also beA. platys,A. phagocytophilum,Anaplasmasp. BL126-13 (KJ410243), andAnaplasmasp. JC3-6 (KM227012). Our study shows that domestic ruminants from China are not uncommonly infected with a variety ofAnaplasmaspp. andEhrlichiaspp.
authors.affiliation: Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University College of Veterinary Medicine, Yangzhou, Jiangsu 225009, China.
authors.forename: Haixiang
authors.initials: H
authors.lastname: Qiu
authors.affiliation: Ross University School of Veterinary Medicine, Basseterre 00265, Saint Kitts and Nevis.
authors.forename: Patrick John
authors.initials: PJ
authors.lastname: Kelly
authors.affiliation: Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University College of Veterinary Medicine, Yangzhou, Jiangsu 225009, China.
authors.forename: Jilei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University College of Veterinary Medicine, Yangzhou, Jiangsu 225009, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University College of Veterinary Medicine, Yangzhou, Jiangsu 225009, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Yangzhou University College of Animal Science and Technology, Yangzhou, Jiangsu 225009, China.
authors.forename: Yongjiang
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Yangzhou University College of Animal Science and Technology, Yangzhou, Jiangsu 225009, China.
authors.forename: Zhangping
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University College of Veterinary Medicine, Yangzhou, Jiangsu 225009, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Alabama State University, Montgomery, AL 36104, USA.
authors.forename: Hongzhuan
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Pathobiology, Auburn University College of Veterinary Medicine, Auburn, AL 36849, USA.
authors.forename: Chengming
authors.identifier: 0000-0001-7874-8340
authors.initials: C
authors.lastname: Wang