title: Intraarterial route increases the risk of cerebral lesions after mesenchymal cell administration in animal model of ischemia.
abstract: Mesenchymal stem cells (MSCs) are a promising clinical therapy for ischemic stroke. However, critical parameters, such as the most effective administration route, remain unclear. Intravenous (i.v.) and intraarterial (i.a.) delivery routes have yielded varied outcomes across studies, potentially due to the unknown MSCs distribution. We investigated whether MSCs reached the brain following i.a. or i.v. administration after transient cerebral ischemia in rats, and evaluated the therapeutic effects of both routes. MSCs were labeled with dextran-coated superparamagnetic nanoparticles for magnetic resonance imaging (MRI) cell tracking, transmission electron microscopy and immunohistological analysis. MSCs were found in the brain following i.a. but not i.v. administration. However, the i.a. route increased the risk of cerebral lesions and did not improve functional recovery. The i.v. delivery is safe but MCS do not reach the brain tissue, implying that treatment benefits observed for this route are not attributable to brain MCS engrafting after stroke.
authors.affiliation: Clinical Neurosciences Research Laboratory, Clinical University Hospital, Health Research Institute of Santiago de Compostela (IDIS), Universidade de Santiago de Compostela, Santiago de Compostela, Spain.
authors.forename: Bárbara
authors.initials: B
authors.lastname: Argibay
authors.affiliation: IMEC, Department of Life Science Technology, Leuven 3001, Belgium.|Biomedical MRI, Department of Imaging and Pathology, KU Leuven, Leuven 3000, Belgium.
authors.forename: Jesse
authors.initials: J
authors.lastname: Trekker
authors.affiliation: Biomedical MRI, Department of Imaging and Pathology, KU Leuven, Leuven 3000, Belgium.
authors.forename: Uwe
authors.initials: U
authors.lastname: Himmelreich
authors.affiliation: Department of Morphological Sciences, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.
authors.forename: Andrés
authors.initials: A
authors.lastname: Beiras
authors.affiliation: Grupo de Física de Coloides y Polímeros, Departamento de Física de la Materia Condensada, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.|Departamento de Fisiología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara 44340, México.
authors.forename: Antonio
authors.initials: A
authors.lastname: Topete
authors.affiliation: Grupo de Física de Coloides y Polímeros, Departamento de Física de la Materia Condensada, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: Taboada
authors.affiliation: Clinical Neurosciences Research Laboratory, Clinical University Hospital, Health Research Institute of Santiago de Compostela (IDIS), Universidade de Santiago de Compostela, Santiago de Compostela, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Pérez-Mato
authors.affiliation: Clinical Neurosciences Research Laboratory, Clinical University Hospital, Health Research Institute of Santiago de Compostela (IDIS), Universidade de Santiago de Compostela, Santiago de Compostela, Spain.
authors.forename: Alba
authors.initials: A
authors.lastname: Vieites-Prado
authors.affiliation: Clinical Neurosciences Research Laboratory, Clinical University Hospital, Health Research Institute of Santiago de Compostela (IDIS), Universidade de Santiago de Compostela, Santiago de Compostela, Spain.
authors.forename: Ramón
authors.initials: R
authors.lastname: Iglesias-Rey
authors.affiliation: Applied Physics Department, Campus Vida, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Rivas
authors.affiliation: Department of Brain Ischemia and Neurodegeneration, Institut d' Investigacions Biomèdiques de Barcelona (IIBB), Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, Spain.|August Pi i Sunyer Biomedical Research Institute (IDIBAPS), Barcelona, Spain.
authors.forename: Anna M
authors.initials: AM
authors.lastname: Planas
authors.affiliation: Clinical Neurosciences Research Laboratory, Clinical University Hospital, Health Research Institute of Santiago de Compostela (IDIS), Universidade de Santiago de Compostela, Santiago de Compostela, Spain.
authors.forename: Tomás
authors.initials: T
authors.lastname: Sobrino
authors.affiliation: Clinical Neurosciences Research Laboratory, Clinical University Hospital, Health Research Institute of Santiago de Compostela (IDIS), Universidade de Santiago de Compostela, Santiago de Compostela, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Castillo
authors.affiliation: Clinical Neurosciences Research Laboratory, Clinical University Hospital, Health Research Institute of Santiago de Compostela (IDIS), Universidade de Santiago de Compostela, Santiago de Compostela, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Campos
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000000981:diagnostic imaging / Q000473:pathology* / Q000534:rehabilitation / Q000628:therapy; D058948:Cell Tracking; D002540:Cerebral Cortex / Q000378:metabolism / Q000473:pathology; D003911:Dextrans; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells; D051381:Rats; D020127:Recovery of Function
chemical_list: D003911:Dextrans; D058185:Magnetite Nanoparticles