title: A cell-permeable and triazole-forming fluorescent probe for glycoconjugate imaging in live cells.
abstract: A new fluorescence-forming probe, coumOCT, designed by fusing cyclooctyne with a coumarin fluorophore was successfully used for the imaging of azido-glycoconjugates in living HeLa cells. This probe is cell-permeable and generates fluorescence after triazole formation, thus minimizing the background signal and enabling the real-time intracellular imaging of glycoconjugate trafficking.
authors.affiliation: Institute of Chemistry, Academia Sinica, 128 Academia Road Section 2, Nankang, Taipei 11529, Taiwan. shiejj@gate.sinica.edu.tw.
authors.forename: Jiun-Jie
authors.identifier: http://orcid.org/0000-0003-0856-214X
authors.initials: JJ
authors.lastname: Shie
authors.affiliation: Genomics Research Center, Academia Sinica, 128 Academia Road Section 2, Nankang, Taipei 11529, Taiwan. chwong@gate.sinica.edu.tw.
authors.forename: Ying-Chih
authors.initials: YC
authors.lastname: Liu
authors.affiliation: Institute of Chemistry, Academia Sinica, 128 Academia Road Section 2, Nankang, Taipei 11529, Taiwan. shiejj@gate.sinica.edu.tw.
authors.forename: Jye-Chian
authors.initials: JC
authors.lastname: Hsiao
authors.affiliation: Genomics Research Center, Academia Sinica, 128 Academia Road Section 2, Nankang, Taipei 11529, Taiwan. chwong@gate.sinica.edu.tw and Department of Chemistry, National Taiwan University, 1 Roosevelt Road Section 4, Taipei 10617, Taiwan.
authors.forename: Jim-Min
authors.initials: JM
authors.lastname: Fang
authors.affiliation: Genomics Research Center, Academia Sinica, 128 Academia Road Section 2, Nankang, Taipei 11529, Taiwan. chwong@gate.sinica.edu.tw and Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors.forename: Chi-Huey
authors.identifier: http://orcid.org/0000-0002-9961-7865
authors.initials: CH
authors.lastname: Wong
mesh_terms: D002470:Cell Survival; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006001:Glycoconjugates / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D015394:Molecular Structure; D061848:Optical Imaging; D014230:Triazoles / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D006001:Glycoconjugates; D014230:Triazoles