title: The Zebrafish Anillin-eGFP Reporter Marks Late Dividing Retinal Precursors and Stem Cells Entering Neuronal Lineages.
abstract: Monitoring cycling behaviours of stem and somatic cells in the living animal is a powerful tool to better understand tissue development and homeostasis. The tg(anillin:anillin-eGFP) transgenic line carries the full-length zebrafish F-actin binding protein Anillin fused to eGFP from a bacterial artificial chromosome (BAC) containing Anillin cis-regulatory sequences. Here we report the suitability of the Anillin-eGFP reporter as a direct indicator of cycling cells in the late embryonic and post-embryonic retina. We show that combining the anillin:anillin-eGFP with other transgenes such as ptf1a:dsRed and atoh7:gap-RFP allows obtaining spatial and temporal resolution of the mitotic potentials of specific retinal cell populations. This is exemplified by the analysis of the origin of the previously reported apically and non-apically dividing late committed precursors of the photoreceptor and horizontal cell layers.
authors.affiliation: Centre for Organismal Studies, Heidelberg University, Heidelberg, Germany.
authors.forename: Meret
authors.initials: M
authors.lastname: Cepero Malo
authors.affiliation: Centre for Organismal Studies, Heidelberg University, Heidelberg, Germany.
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Duchemin
authors.affiliation: Department of Cell and Molecular Biology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
authors.forename: Luca
authors.initials: L
authors.lastname: Guglielmi
authors.affiliation: Centre for Organismal Studies, Heidelberg University, Heidelberg, Germany.
authors.forename: Eva
authors.initials: E
authors.lastname: Patzel
authors.affiliation: The David J Apple Center for Vision Research, Department of Ophthalmology, Heidelberg University Hospital, Heidelberg, Germany.
authors.forename: Saadettin
authors.initials: S
authors.lastname: Sel
authors.affiliation: The David J Apple Center for Vision Research, Department of Ophthalmology, Heidelberg University Hospital, Heidelberg, Germany.
authors.forename: Gerd U
authors.initials: GU
authors.lastname: Auffarth
authors.affiliation: Department of Cell and Molecular Biology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Carl
authors.affiliation: Centre for Organismal Studies, Heidelberg University, Heidelberg, Germany.|The David J Apple Center for Vision Research, Department of Ophthalmology, Heidelberg University Hospital, Heidelberg, Germany.
authors.forename: Lucia
authors.initials: L
authors.lastname: Poggi
mesh_terms: D000818:Animals; D002455:Cell Division; D019070:Cell Lineage; D003285:Contractile Proteins / Q000235:genetics*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics*; D009474:Neurons / Q000166:cytology*; D012160:Retina / Q000166:cytology*; D015027:Zebrafish / Q000235:genetics*
chemical_list: D003285:Contractile Proteins; C095886:anillin; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins