title: Isolation, Colonization, and Chlorpyrifos Degradation Mediation of the Endophytic Bacterium Sphingomonas Strain HJY in Chinese Chives (Allium tuberosum).
abstract: The endophyte-plant interaction can benefit the host in many different ways. An endophytic bacterium strain (HJY) capable of degrading chlorpyrifos (CP) was isolated from Chinese chives (Allium tuberosum Rottl. ex Spreng). The isolated bacterium HJY classified as Sphingomonas sp. strain HJY could use CP as the sole carbon source. After being marked with the gfp gene, the colonization and distribution of strain HJY-gfp were directly observed in different tissues of Chinese chives with a confocal laser scanning microscope. The inoculation of strain HJY-gfp in Chinese chives resulted in a higher degradation of CP inside the plants than in uninoculated plants. With drench application, up to 70 and 66% of CP were removed from shoots and roots of inoculated Chinese chives, respectively. Moreover, up to 75% of CP was removed from the soil containing plants inoculated with HJY-gfp. With foliage application, the applied concentration of chlorpyrifos affected the degradation performance of strain HJY in Chinese chives. Significant differences were observed only between inoculated and uninoculated Chinese chives with the low applied concentration of CP. Together, other than natural endophyte-assisted plant protection for food safety, the interaction of HJY and plant may be also a promising strategy for in situ bioremediation of soil contaminated with CP.
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province- State Key Laboratory Breeding Base , 50 Zhongling Street, Nanjing 210014, China.|Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences , 50 Zhongling Street, Nanjing 210014, China.
authors.forename: Fayun
authors.initials: F
authors.lastname: Feng
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province- State Key Laboratory Breeding Base , 50 Zhongling Street, Nanjing 210014, China.|Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences , 50 Zhongling Street, Nanjing 210014, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ge
authors.affiliation: Department of Plant Protection, Agricultural College, Shihezi University , Xinjiang 832000, China.
authors.forename: Yisong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province- State Key Laboratory Breeding Base , 50 Zhongling Street, Nanjing 210014, China.
authors.forename: Jinjin
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences , 50 Zhongling Street, Nanjing 210014, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province- State Key Laboratory Breeding Base , 50 Zhongling Street, Nanjing 210014, China.|Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences , 50 Zhongling Street, Nanjing 210014, China.
authors.forename: Xiangyang
authors.identifier: http://orcid.org/0000-0002-2831-172X
authors.initials: X
authors.lastname: Yu
mesh_terms: D001673:Biodegradation, Environmental; D027782:Chive / Q000382:microbiology*; D004390:Chlorpyrifos / Q000493:pharmacokinetics*; D003470:Culture Media; D060026:Endophytes / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics; D018515:Plant Leaves; D020578:Sphingomonas / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D003470:Culture Media; D049452:Green Fluorescent Proteins; D004390:Chlorpyrifos
keywords: Sphigomonas sp.; bioremediation; chlorpyrifos; degradation; endophyte−host interaction; food safety