title: Description of Auricoccus indicus gen. nov., sp. nov., isolated from skin of human ear.
abstract: A Gram-stain-positive, non-motile, non-spore-forming, small spherical bacterium, strain S31T, was isolated from skin surface (external ear lobe) of a healthy human subject and characterized using a polyphasic approach. On the basis of 1507 bp 16S rRNA gene sequence comparison, S31T showed highest (92.8 %, AY119686) sequence similarity with Macrococcus brunensis CCUG 47200T followed by Macrococcus caseolyticus DSM 20597T (92.7 % AP009484) and formed a separate clade with 65 % bootstrap support. The DNA G+C content was found to be 34 mol%. Anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0 are the predominant fatty acids in fatty acid methyl ester (FAME) profile of strain S31T. It contained A3α type peptidoglycan with l-Lys-Gly3-l-Ala peptide. Comparative study of morphological and physiological traits indicated that S31T has phenetically diverged from its closest relatives. On the basis of morphological, chemotaxonomic and genotypic data, S31T showed marked distinctions from its closest relatives of the family Staphylococcaceae and is proposed to represent a novel genus Auricoccus with Auricoccus indicus as type species of the genus. S31T (CCUG 69858T=KCTC 33611T=MCC 3027T) is the type strain of the species.
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pune 411007, Maharashtra, India.
authors.forename: Om
authors.initials: O
authors.lastname: Prakash
authors.affiliation: CSIR-Institute of Genomics and Integrative Biology, Council of Scientific and Industrial Research (CSIR), New Delhi 110020, India.
authors.forename: Suchismita
authors.initials: S
authors.lastname: Muduli
authors.affiliation: CSIR-Institute of Genomics and Integrative Biology, Council of Scientific and Industrial Research (CSIR), New Delhi 110020, India.
authors.forename: Rohit
authors.initials: R
authors.lastname: Kumar
authors.affiliation: CSIR-Institute of Genomics and Integrative Biology, Council of Scientific and Industrial Research (CSIR), New Delhi 110020, India.
authors.forename: Chanchal
authors.initials: C
authors.lastname: Kumari
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pune 411007, Maharashtra, India.
authors.forename: Yogesh
authors.initials: Y
authors.lastname: Nimonkar
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pune 411007, Maharashtra, India.
authors.forename: Yogesh S
authors.initials: YS
authors.lastname: Shouche
authors.affiliation: CSIR-Institute of Genomics and Integrative Biology, Council of Scientific and Industrial Research (CSIR), New Delhi 110020, India.|Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi 110001, India.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Sharma
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D004423:Ear / Q000382:microbiology*; D005227:Fatty Acids / Q000737:chemistry; D006093:Gram-Positive Asporogenous Rods / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012867:Skin / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S