title: Dose-dependency and reversibility of radiation-induced injury in cardiac explant-derived cells of mice.
abstract: We evaluated the dose-dependency and reversibility of radiation-induced injury in cardiac explant-derived cells (CDCs), a mixed cell population grown from heart tissues. Adult C57BL/6 mice were exposed to 0, 10, 50 and 250 mGy γ-rays for 7 days and atrial tissues were collected for experiments 24 hours after last exposure. The number of CDCs was significantly decreased by daily exposure to over 250 mGy. Interestingly, daily exposure to over 50 mGy significantly decreased the c-kit expression and telomerase activity, increased 53BP1 foci in the nuclei of CDCs. However, CD90 expression and growth factors production in CDCs were not significantly changed even after daily exposure to 250 mGy. We further evaluated the reversibility of radiation-induced injury in CDCs at 1 week and 3 weeks after a single exposure to 3 Gy γ-rays. The number and growth factors production of CDCs were soon recovered at 1 week. However, the increased expression of CD90 were retained at 1 week, but recovered at 3 weeks. Moreover, the decreased expression of c-kit, impaired telomerase activity, and increased 53BP1 foci were poorly recovered even at 3 weeks. These data may help us to find the most sensitive and reliable bio-parameter(s) for evaluating radiation-induced injury in CDCs.
authors.affiliation: Department of Stem Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
authors.forename: Lan
authors.initials: L
authors.lastname: Luo
authors.affiliation: Department of Stem Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
authors.forename: Chen
authors.initials: C
authors.lastname: Yan
authors.affiliation: Department of Stem Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
authors.forename: Yoshishige
authors.initials: Y
authors.lastname: Urata
authors.affiliation: Department of Stem Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
authors.forename: Al Shaimaa
authors.initials: AS
authors.lastname: Hasan
authors.affiliation: Department of Stem Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Goto
authors.affiliation: Jiangxi Cancer Hospital, Nanchang, Jiangxi 330029, PR China.
authors.forename: Chang-Ying
authors.initials: CY
authors.lastname: Guo
authors.affiliation: Department of General Surgery, Jiangxi Provincial Children's Hospital, Nanchang, Jiangxi Province, 330006, PR China.
authors.forename: Shouhua
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Stem Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
authors.forename: Tao-Sheng
authors.initials: TS
authors.lastname: Li
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000528:radiation effects; D002477:Cells / Q000528:radiation effects*; D004797:Enzyme-Linked Immunosorbent Assay; D020869:Gene Expression Profiling; D006321:Heart / Q000528:radiation effects*; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000166:cytology*; D011829:Radiation Dosage; D011832:Radiation Injuries / Q000473:pathology*