title: Staphylococcus aureusLpl Lipoproteins Delay G2/M Phase Transition in HeLa Cells.
abstract: The cell cycle is an ordered set of events, leading to cell growth and division into two daughter cells. The eukaryotic cell cycle consists of interphase (G1, S, and G2phases), followed by the mitotic phase and G0phase. Many bacterial pathogens secrete cyclomodulins that interfere with the host cell cycle. InStaphylococcus aureusfour cyclomodulins have been described so far that all represent toxins and are secreted into the culture supernatant. Here we show that the membrane-anchored lipoprotein-like proteins (Lpl), encoded on a genomic island called νSaα, interact with the cell cycle of HeLa cells. By comparing wild type andlpldeletion mutant it turned out that thelplcluster is causative for the G2/M phase transition delay and also contributes to increased invasion frequency. The lipoprotein Lpl1, a representative of thelplcluster, also caused G2/M phase transition delay. Interestingly, the lipid modification, which is essential for TLR2 signaling and activation of the immune system, is not necessary for cyclomodulin activity. Unlike the other staphylococcal cyclomodulins Lpl1 shows no cytotoxicity even at high concentrations. As all Lpl proteins are highly conserved there might be a common function that is accentuated by their multiplicity in a tandem gene cluster. The cell surface localized Lpls' suggests a correlation between G2/M phase transition delay and host cell invasion.
authors.affiliation: Microbial Genetics, Interfaculty Institute of Microbiology and Infection Medicine Tübingen, University of Tübingen Tübingen, Germany.
authors.forename: Minh-Thu
authors.initials: MT
authors.lastname: Nguyen
authors.affiliation: Institut National de la Recherche Agronomique (INRA), UMR1253 Science & Technologie du Lait & de l'Oeuf (STLO) Rennes, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Deplanche
authors.affiliation: Microbial Genetics, Interfaculty Institute of Microbiology and Infection Medicine Tübingen, University of Tübingen Tübingen, Germany.
authors.forename: Mulugeta
authors.initials: M
authors.lastname: Nega
authors.affiliation: Institut National de la Recherche Agronomique (INRA), UMR1253 Science & Technologie du Lait & de l'Oeuf (STLO) Rennes, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Le Loir
authors.affiliation: Microbial Genetics, Interfaculty Institute of Microbiology and Infection Medicine Tübingen, University of Tübingen Tübingen, Germany.
authors.forename: Loulou
authors.initials: L
authors.lastname: Peisl
authors.affiliation: Microbial Genetics, Interfaculty Institute of Microbiology and Infection Medicine Tübingen, University of Tübingen Tübingen, Germany.
authors.forename: Friedrich
authors.initials: F
authors.lastname: Götz
authors.affiliation: Institut National de la Recherche Agronomique (INRA), UMR1253 Science & Technologie du Lait & de l'Oeuf (STLO) Rennes, France.
authors.forename: Nadia
authors.initials: N
authors.lastname: Berkova
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle; D004847:Epithelial Cells / Q000382:microbiology* / Q000473:pathology*; D017353:Gene Deletion; D006367:HeLa Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism*; D013203:Staphylococcal Infections / Q000382:microbiology / Q000473:pathology*; D013211:Staphylococcus aureus / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D008074:Lipoproteins
keywords: G2/M phase; Staphylococcus aureus; cell cycle; lipopeptides; lipoproteins