title: The application of phenotypic microarray analysis to anti-fungal drug development.
abstract: Candida albicans metabolic activity in the presence and absence of acetylcholine was measured using phenotypic microarray analysis. Acetylcholine inhibited C. albicans biofilm formation by slowing metabolism independent of biofilm forming capabilities. Phenotypic microarray analysis can therefore be used for screening compound libraries for novel anti-fungal drugs and measuring antifungal resistance.
authors.affiliation: School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, UK.
authors.forename: Darren
authors.initials: D
authors.lastname: Greetham
authors.affiliation: Oral Sciences Research Group, Glasgow Dental School, School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, University of Glasgow, UK.
authors.forename: David F
authors.initials: DF
authors.lastname: Lappin
authors.affiliation: Oral Sciences Research Group, Glasgow Dental School, School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, University of Glasgow, UK.
authors.forename: Ranjith
authors.initials: R
authors.lastname: Rajendran
authors.affiliation: Oral Sciences Research Group, Glasgow Dental School, School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, University of Glasgow, UK.
authors.forename: Lindsay
authors.initials: L
authors.lastname: O'Donnell
authors.affiliation: Oral Sciences Research Group, Glasgow Dental School, School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, University of Glasgow, UK.
authors.forename: Leighann
authors.initials: L
authors.lastname: Sherry
authors.affiliation: Oral Sciences Research Group, Glasgow Dental School, School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, University of Glasgow, UK.
authors.forename: Gordon
authors.initials: G
authors.lastname: Ramage
authors.affiliation: Oral Sciences Research Group, Glasgow Dental School, School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, University of Glasgow, UK. Electronic address: christopher.nile@glasgow.ac.uk.
authors.forename: Christopher
authors.initials: C
authors.lastname: Nile
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D058387:Candidemia / Q000382:microbiology; D055808:Drug Discovery / Q000379:methods*; D046228:Microarray Analysis / Q000379:methods*; D010641:Phenotype
chemical_list: D000935:Antifungal Agents; D000109:Acetylcholine
keywords: Acetylcholine; Anti-fungal; Candida albicans; Metabolism; Phenotypic microarray; Resistance