title: Plasma mannose level, a putative indicator of glycogenolysis, and glucose tolerance in Japanese individuals.
abstract: OBJECTIVE
  Mannose is a monosaccharide constituent of glycoproteins and glycolipids. Experiments in rats have shown previously that the plasma mannose level decreases after glucose load, but does not decrease in diabetic rats, and that hepatic glycogenolysis is a source of this plasma mannose; however, these results are not fully elucidated in humans. Plasma mannose levels before/after glucose loading in humans with various degrees of glucose intolerance were examined to analyze their association with clinical factors.
  
  
  METHODS
  The 75-g oral glucose tolerance test was carried out in Japanese individuals not taking diabetes medications. Participants were classified into normal glucose tolerance, impaired glucose metabolism and diabetes mellitus groups. Insulinogenic index as an index of insulin secretion, and Matsuda Index as an index of insulin sensitivity were calculated. Mannose was assayed by the established method using high-performance liquid chromatography after labeling.
  
  
  RESULTS
  After glucose load, the plasma mannose level decreased gradually in the normal glucose tolerance group, but did not decrease in the diabetes mellitus group. Plasma mannose changes during 120 min from baseline (M120-M0) were significantly different among the three groups (normal glucose tolerance: -16.7 ± 1.7; impaired glucose metabolism: -9.0 ± 1.9; diabetes mellitus: -1.4 ± 1.8 μmol/L [n = 25 in each group], P < 0.0001). Plasma glucose 120 min after glucose loading (R2= 0.412) or loge-insulinogenic index, loge-Matsuda Index and age (R2= 0.230) were determinants of M120-M0in multiple regression analyses.
  
  
  CONCLUSIONS
  We clarified the relationship between plasma mannose level and glucose tolerance in humans. The present results are compatible with those using rats, in which mannose derived from glycogenolysis plays an important role in the alteration of mannose levels after glucose loading.
authors.affiliation: Department of Endocrinology, Metabolism and Nephrology, Kochi Medical School, Kochi University, Nankoku, Japan.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Yoshimura
authors.affiliation: Department of Endocrinology, Metabolism and Nephrology, Kochi Medical School, Kochi University, Nankoku, Japan.
authors.forename: Seiki
authors.initials: S
authors.lastname: Hirano
authors.affiliation: Department of Endocrinology, Metabolism and Nephrology, Kochi Medical School, Kochi University, Nankoku, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takata
authors.affiliation: Department of Endocrinology, Metabolism and Nephrology, Kochi Medical School, Kochi University, Nankoku, Japan.
authors.forename: Shogo
authors.initials: S
authors.lastname: Funakoshi
authors.affiliation: Department of Endocrinology, Metabolism and Nephrology, Kochi Medical School, Kochi University, Nankoku, Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Ohmi
authors.affiliation: Department of Endocrinology, Metabolism and Nephrology, Kochi Medical School, Kochi University, Nankoku, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Amano
authors.affiliation: Department of Endocrinology, Metabolism and Nephrology, Kochi Medical School, Kochi University, Nankoku, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Nishi
authors.affiliation: Department of Endocrinology, Metabolism and Nephrology, Kochi Medical School, Kochi University, Nankoku, Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Inoue
authors.affiliation: Fukuda Clinic, Kochi, Japan.
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Fukuda
authors.affiliation: Department of Pathobiochemistry, Faculty of Pharmacy, Meijo University, Nagoya, Japan.
authors.forename: Hikari
authors.initials: H
authors.lastname: Hayashi
authors.affiliation: Department of Pathobiochemistry, Faculty of Pharmacy, Meijo University, Nagoya, Japan.
authors.forename: Tadao
authors.initials: T
authors.lastname: Taguchi
authors.affiliation: Department of Pathobiochemistry, Faculty of Pharmacy, Meijo University, Nagoya, Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Yamada
authors.affiliation: Department of Pathobiochemistry, Faculty of Pharmacy, Meijo University, Nagoya, Japan.
authors.forename: Ichitomo
authors.initials: I
authors.lastname: Miwa
authors.affiliation: Department of Endocrinology, Metabolism and Nephrology, Kochi Medical School, Kochi University, Nankoku, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Terada
authors.affiliation: Department of Endocrinology, Metabolism and Nephrology, Kochi Medical School, Kochi University, Nankoku, Japan.
authors.forename: Shimpei
authors.initials: S
authors.lastname: Fujimoto
mesh_terms: D000368:Aged; D005260:Female; D018149:Glucose Intolerance; D005951:Glucose Tolerance Test; D050261:Glycogenolysis; D006801:Humans; D008297:Male; D008358:Mannose / Q000097:blood*; D008875:Middle Aged; D012044:Regression Analysis
chemical_list: D008358:Mannose
keywords: Glucose tolerance; Glycogenolysis; Mannose