title: A novel polysaccharide with antioxidant, HIV protease inhibiting and HIV integrase inhibiting activities from Fomitiporia punctata (P. karst.) murrill (Basidiomycota, hymenochaetales).
abstract: A novel polysaccharide fraction (G1) was obtained from the fungus Fomitiporia punctata (P. Karst.) Murrill. G1exhibited a molecular weight of approximately 151kDa. The FT-IR results suggested that the monosaccharide components of G1 possessed furanoid rings and there were β-glycosidic bonds between the sugar units. The1H NMR results showed that G1 was composed of arabinose, fructose, galactose and glucose in the molar ratio of 1.6:3.8:19.7:19.7, as determined by gas chromatography-mass spectrometry (GC-MS) and high performance liquid chromatography (HPLC). G1produced significant antioxidant effects as evidenced by its potency in inhibiting erythrocyte hemolysis, and in scavenging hydroxyl radicals and superoxide radicals. The highest rates of inhibition achieved were 73.58%, 36.55% and 50.98% respectively. In addition, G1brought about 19.6% inhibition of HIV-1 protease activity at the concentration of 50μg/mL. G1 displayed inhibitory activity toward HIV-1 integrase in the concentration range of 100-1000μg/mL. The present study indicates that G1from Fomitiporia punctate (P. Karst.) Murrill is a novel natural antioxidant.
authors.affiliation: The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin 300071, China.
authors.forename: Faaang
authors.initials: F
authors.lastname: Liu
authors.affiliation: Zibo Center for Disease Control and Prevention, Zibo 255026, Shandong, China.
authors.forename: Yinping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin 300071, China.
authors.forename: Keren
authors.initials: K
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin 300071, China.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin 300071, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhou
authors.affiliation: The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin 300071, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin 300071, China.
authors.forename: Yajie
authors.initials: Y
authors.lastname: Han
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China. Electronic address: b021770@mailserv.cuhk.edu.hk.
authors.forename: Tzi Bun
authors.initials: TB
authors.lastname: Ng
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001487:Basidiomycota / Q000737:chemistry*; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D019427:HIV Integrase / Q000378:metabolism*; D019428:HIV Integrase Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D016333:HIV Protease / Q000378:metabolism*; D017320:HIV Protease Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D062610:Fungal Polysaccharides; D019428:HIV Integrase Inhibitors; D017320:HIV Protease Inhibitors; D019427:HIV Integrase; D016333:HIV Protease
keywords: Antioxidant; Fomitiporia punctata; HIV IntegraseInhibiting; HIV protease inhibiting; Mushroom; Polysaccharide