title: Evaluation of sperm quality of Erythrolamprus poecilogyrus sublineatus (Cope, 1860) (Serpentes, Dipsadidae).
abstract: Erythrolamprus poecilogyrus sublineatus (Cope, 1860), is a species widely distributed in the Pampa Domain, occurring in Rio Grande do Sul, Argentina and Uruguay, mainlyin the pampa region. In the coastal region of southern Brazil this is serpent is considered one of the most abundant. The purpose of the present study is to describe the techniques of sperm evaluation in vitro for E. poecilogyrus sublineatus in the coastal plain of Rio Grande do Sul, Brazil. After laparatomy the efferent vases were collected and the semen was diluted in 1ml Beltsville Thawing Solution. The characteristics of motility, membrane integrity, mitochondria, acrosome, DNA, cell viability and cellular functionality were evaluated. Fluorescent probes were used for the evaluation of sperm structure in epifluorescence microscope. With the techniques described, it was possible to identify intact and injured cells, enabling the determination of cell characteristics for the spring season (October and November). It was observed in the analyses that 80% of sperm cells were mobile and that 84.1 ± 8.0% of sperm membranes were intact. The standards found were of 48 ± 13.8% of intact acrosome, 73.6 ± 6.0 of perfect DNA and of 91.8 ± 4.0 of functional mitochondria. Thus, these values from the sperm analysis can be used as standards for the species Erythrolamprus poecilogyrus sublineatus.
authors.affiliation: Center for Research and Education in Animal Reproduction, Faculdade de Veterinária, Universidade Federal de Pelotas - UFPel, Campus Universitário, s/n, CEP 96160-000, Capão do Leão, RS, Brazil.
authors.forename: A C
authors.initials: AC
authors.lastname: Silva
authors.affiliation: Institute of Biological Sciences, Universidade Federal do Rio Grande - FURG, Campus Carreiros, Av. Itália, Km 8, Bairro Carreiros, CEP 96203-900, Rio Grande, RS, Brazil.
authors.forename: A S
authors.initials: AS
authors.lastname: Varela
authors.affiliation: Institute of Biological Sciences, Universidade Federal do Rio Grande - FURG, Campus Carreiros, Av. Itália, Km 8, Bairro Carreiros, CEP 96203-900, Rio Grande, RS, Brazil.
authors.forename: T F
authors.initials: TF
authors.lastname: Cardoso
authors.affiliation: Institute of Biological Sciences, Universidade Federal do Rio Grande - FURG, Campus Carreiros, Av. Itália, Km 8, Bairro Carreiros, CEP 96203-900, Rio Grande, RS, Brazil.
authors.forename: E F
authors.initials: EF
authors.lastname: Silva
authors.affiliation: Institute of Biological Sciences, Universidade Federal do Rio Grande - FURG, Campus Carreiros, Av. Itália, Km 8, Bairro Carreiros, CEP 96203-900, Rio Grande, RS, Brazil.
authors.forename: D
authors.initials: D
authors.lastname: Loebmann
authors.affiliation: Center for Research and Education in Animal Reproduction, Faculdade de Veterinária, Universidade Federal de Pelotas - UFPel, Campus Universitário, s/n, CEP 96160-000, Capão do Leão, RS, Brazil.
authors.forename: C D
authors.initials: CD
authors.lastname: Corcini
mesh_terms: D000818:Animals; D001938:Brazil; D004542:Ejaculation; D008297:Male; D055101:Semen Analysis; D012911:Snakes / Q000502:physiology*; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*