title: Potential applications of lipid peroxidation products - F4-neuroprostanes, F3-neuroprostanesn-6 DPA, F2-dihomo-isoprostanes and F2-isoprostanes - in the evaluation of the allograft function in renal transplantation.
abstract: F4-neuroprostanes, F3-neuroprostanesn-6 DPA, and F2-dihomo-isoprostanes, metabolites of non-enzymatic lipid peroxidation of polyunsaturated fatty acids [docosahexaenoic acid, n-6 docosapentanoic acid, and adrenic acid respectively], have become important biomarkers for oxidative stress in several diseases like epilepsy and alzheimer. These biomarkers and the 15-F2t-isoprostane (also known as 8-iso-PGF2α),a F2-isoprostane isomer measured as reference oxidative marker at systemic level, were analyzed by UHPLC-QqQ-MS/MS in the urine of 60 renal recipients from cadaveric donors of the Nephrology Unit of the University Hospital Virgen de la Arrixaca, at six different times during the first six months after renal transplantation, and were compared with a control group of 60 healthy subjects from the same hospital. A total of 11 metabolites were analyzed and different patterns were observed. A tendency to decrease was observed in three metabolites (4-epi-4-F3t- NeuroPn-6 DPA, ent-7(RS)-7-F2t-dihomo-IsoP, and ent-7(S)-7-F2t-dihomo-IsoP) and in our reference oxidative marker (15-F2t-IsoP) when kidney function improved and the excretion of urine proteins decreased. These results suggest that these three biomarkers of oxidative stress could be useful to assess renal function in the postransplant phase. Unfortunately, little is known about this kind of biomarker in this cohort of patients, so further investigation would be required in the clinical field to clarify the relationship between oxidative stress and the graft function, as well as the usefulness of these biomarkers as rejection markers.
authors.affiliation: Clinical Analysis Service, University Hospital Virgen de la Arrixaca, Ctra. Madrid-Cartagena, s/n, 30120, El Palmar, Murcia, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: De Las Heras-Gómez
authors.affiliation: Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100 Campus University Espinardo, Murcia, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Medina
authors.affiliation: Clinical Analysis Service, University Hospital Virgen de la Arrixaca, Ctra. Madrid-Cartagena, s/n, 30120, El Palmar, Murcia, Spain. Electronic address: tcasaspina@gmail.com.
authors.forename: Teresa
authors.initials: T
authors.lastname: Casas-Pina
authors.affiliation: Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100 Campus University Espinardo, Murcia, Spain.
authors.forename: Lidia
authors.initials: L
authors.lastname: Marín-Soler
authors.affiliation: Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100 Campus University Espinardo, Murcia, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Tomás
authors.affiliation: Clinical Analysis Service, University Hospital Virgen de la Arrixaca, Ctra. Madrid-Cartagena, s/n, 30120, El Palmar, Murcia, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Martínez-Hernández
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 - CNRS - University of Montpellier - ENSCM, Faculty of Pharmacy, Montpellier, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Oger
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 - CNRS - University of Montpellier - ENSCM, Faculty of Pharmacy, Montpellier, France.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Galano
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 - CNRS - University of Montpellier - ENSCM, Faculty of Pharmacy, Montpellier, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Durand
authors.affiliation: Nefrology Service, University Hospital Virgen de la Arrixaca, Ctra. Madrid-Cartagena, s/n, 30120, El Palmar, Murcia, Spain.
authors.forename: Luisa
authors.initials: L
authors.lastname: Jimeno
authors.affiliation: Nefrology Service, University Hospital Virgen de la Arrixaca, Ctra. Madrid-Cartagena, s/n, 30120, El Palmar, Murcia, Spain.
authors.forename: Santiago
authors.initials: S
authors.lastname: Llorente
authors.affiliation: Clinical Analysis Service, University Hospital Virgen de la Arrixaca, Ctra. Madrid-Cartagena, s/n, 30120, El Palmar, Murcia, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Lozoya
authors.affiliation: Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100 Campus University Espinardo, Murcia, Spain.
authors.forename: Federico
authors.initials: F
authors.lastname: Ferreres
authors.affiliation: Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100 Campus University Espinardo, Murcia, Spain. Electronic address: angelgil@cebas.csic.es.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Gil-Izquierdo
mesh_terms: D000328:Adult; D000368:Aged; D064591:Allografts; D015415:Biomarkers / Q000652:urine*; D028441:F2-Isoprostanes / Q000652:urine*; D005260:Female; D006801:Humans; D007674:Kidney Diseases / Q000473:pathology / Q000652:urine*; D016030:Kidney Transplantation / Q000009:adverse effects; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D058632:Neuroprostanes / Q000652:urine*; D018384:Oxidative Stress / Q000235:genetics*; D011454:Prostaglandins A / Q000652:urine
chemical_list: C117466:8-isoprostaglandin A2; D015415:Biomarkers; D028441:F2-Isoprostanes; D058632:Neuroprostanes; D011454:Prostaglandins A
keywords: Biomarkers; Dihomo-isoprostanes; Free radicals; Neuroprostanes; Oxidative stress; Renal rejection; Renal transplant; UHPLC-QqQ-MS/MS; Urine