title: Helminth-induced Ly6Chimonocyte-derived alternatively activated macrophages suppress experimental autoimmune encephalomyelitis.
abstract: Helminths cause chronic infections and affect the immune response to unrelated inflammatory diseases. Although helminths have been used therapeutically to ameliorate inflammatory conditions, their anti-inflammatory properties are poorly understood. Alternatively activated macrophages (AAMϕs) have been suggested as the anti-inflammatory effector cells during helminth infections. Here, we define the origin of AAMϕs during infection with Taenia crassiceps, and their disease-modulating activity on the Experimental Autoimmune Encephalomyelitis (EAE). Our data show two distinct populations of AAMϕs, based on the expression of PD-L1 and PD-L2 molecules, resulting upon T. crassiceps infection. Adoptive transfer of Ly6C+monocytes gave rise to PD-L1+/PD-L2+, but not PD-L1+/PD-L2-cells in T. crassiceps-infected mice, demonstrating that the PD-L1+/PD-L2+subpopulation of AAMϕs originates from blood monocytes. Furthermore, adoptive transfer of PD-L1+/PD-L2+AAMϕs into EAE induced mice reduced disease incidence, delayed disease onset, and diminished the clinical disability, indicating the critical role of these cells in the regulation of autoimmune disorders.
authors.affiliation: Department of Pathology, The Ohio State University, Columbus, OH 43221, USA.
authors.forename: Cesar
authors.initials: C
authors.lastname: Terrazas
authors.affiliation: Center for Microbial Pathogenesis, The Research Institute at Nationwide Children's Hospital, Columbus, Ohio, USA.
authors.forename: Juan
authors.initials: J
authors.lastname: de Dios Ruiz-Rosado
authors.affiliation: School of Health and Rehabilitation Sciences, Medical Laboratory Science Division, The Ohio State University, Columbus, Ohio, USA.
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Amici
authors.affiliation: School of Health and Rehabilitation Sciences, Medical Laboratory Science Division, The Ohio State University, Columbus, Ohio, USA.
authors.forename: Kyle A
authors.initials: KA
authors.lastname: Jablonski
authors.affiliation: Unidad de Biomedicina, Facultad de Estudios Superiores-Iztacala, UNAM, Tlalnepantla, MEX, Mexico.
authors.forename: Diana
authors.initials: D
authors.lastname: Martinez-Saucedo
authors.affiliation: School of Health and Rehabilitation Sciences, Medical Laboratory Science Division, The Ohio State University, Columbus, Ohio, USA.|Biomedical Sciences Graduate Program, The Ohio State University, Columbus, Ohio, USA.
authors.forename: Lindsay M
authors.initials: LM
authors.lastname: Webb
authors.affiliation: Center for Microbial Pathogenesis, The Research Institute at Nationwide Children's Hospital, Columbus, Ohio, USA.
authors.forename: Hanna
authors.initials: H
authors.lastname: Cortado
authors.affiliation: Center for Microbial Pathogenesis, The Research Institute at Nationwide Children's Hospital, Columbus, Ohio, USA.
authors.forename: Frank
authors.initials: F
authors.lastname: Robledo-Avila
authors.affiliation: Environmental Health Sciences, College of Public Health, Ohio State University, Columbus, OH, USA.
authors.forename: Steve
authors.initials: S
authors.lastname: Oghumu
authors.affiliation: Department of Pathology, The Ohio State University, Columbus, OH 43221, USA.
authors.forename: Abhay R
authors.initials: AR
authors.lastname: Satoskar
authors.affiliation: Unidad de Biomedicina, Facultad de Estudios Superiores-Iztacala, UNAM, Tlalnepantla, MEX, Mexico.
authors.forename: Miriam
authors.initials: M
authors.lastname: Rodriguez-Sosa
authors.affiliation: Unidad de Biomedicina, Facultad de Estudios Superiores-Iztacala, UNAM, Tlalnepantla, MEX, Mexico.
authors.forename: Luis I
authors.initials: LI
authors.lastname: Terrazas
authors.affiliation: School of Health and Rehabilitation Sciences, Medical Laboratory Science Division, The Ohio State University, Columbus, Ohio, USA.
authors.forename: Mireia
authors.initials: M
authors.lastname: Guerau-de-Arellano
authors.affiliation: Center for Microbial Pathogenesis, The Research Institute at Nationwide Children's Hospital, Columbus, Ohio, USA.|Department of Pediatrics, College of Medicine, The Ohio State University, Columbus, Ohio, USA.
authors.forename: Santiago
authors.initials: S
authors.lastname: Partida-Sánchez
mesh_terms: D019264:Adoptive Transfer / Q000379:methods*; D000818:Animals; D000950:Antigens, Ly / Q000378:metabolism*; D060890:B7-H1 Antigen / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000628:therapy; D005260:Female; D008262:Macrophage Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D055017:Monocyte-Macrophage Precursor Cells / Q000276:immunology*; D061027:Programmed Cell Death 1 Ligand 2 Protein / Q000235:genetics / Q000378:metabolism; D013621:Taenia / Q000276:immunology*
chemical_list: D000950:Antigens, Ly; D060890:B7-H1 Antigen; C498919:Cd274 protein, mouse; C526827:Ly6 protein, mouse; C556978:Pdcd1lg2 protein, mouse; D061027:Programmed Cell Death 1 Ligand 2 Protein