title: Star-shaped copolymer grafted PEI and REDV as a gene carrier to improve migration of endothelial cells.
abstract: In this work, a biodegradable star-shaped copolymer poly(lactide-co-3(S)-methyl-morpholine-2,5-dione)6(Star-(PLMD)6) was synthesized via ring-opening polymerization (ROP), and subsequently a gene carrier Star-PLMD-g-PEI-g-PEG-CREDVW was prepared by grafting polyethyleneimine (PEI), polyethylene glycol (PEG) and targeting peptide REDV onto Star-(PLMD)6. This gene carrier could form stable micelles to condense pEGFP-ZNF580 through electrostatic interaction. The resulting complexes were biocompatible and showed high efficiency in gene delivery. In addition, these complexes exhibited high selectivity for endothelial cells (ECs), high transfection efficiency and enhanced migration of ECs. The protein level of ZNF580 expression was significantly high (up to 85%), while the control group was only 51%. This combination of degradability, targeting ligand and star-structure strategy exhibits a significant advantage in transfection efficiency and migration of ECs.
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Tianjin 300350, China. yakaifeng@tju.edu.cn and Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), Weijin Road 92, Tianjin 300072, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Lv
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Tianjin 300350, China. yakaifeng@tju.edu.cn and Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), Weijin Road 92, Tianjin 300072, China.
authors.forename: Xuefang
authors.initials: X
authors.lastname: Hao
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Tianjin 300350, China. yakaifeng@tju.edu.cn and Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), Weijin Road 92, Tianjin 300072, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Tianjin 300350, China. yakaifeng@tju.edu.cn.
authors.forename: Mary
authors.initials: M
authors.lastname: Akpanyung
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Tianjin 300350, China. yakaifeng@tju.edu.cn.
authors.forename: Abdelilah
authors.initials: A
authors.lastname: Nejjari
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Tianjin 300350, China. yakaifeng@tju.edu.cn.
authors.forename: Agnaldo Luis
authors.initials: AL
authors.lastname: Neve
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Tianjin 300350, China. yakaifeng@tju.edu.cn and Tianjin University-Helmholtz-Zentrum Geesthacht, Joint Laboratory for Biomaterials and Regenerative Medicine, Yaguan Road 135, Tianjin 300350, China.
authors.forename: Xiangkui
authors.initials: X
authors.lastname: Ren
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Tianjin 300350, China. yakaifeng@tju.edu.cn and Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), Weijin Road 92, Tianjin 300072, China and Tianjin University-Helmholtz-Zentrum Geesthacht, Joint Laboratory for Biomaterials and Regenerative Medicine, Yaguan Road 135, Tianjin 300350, China and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Weijin Road 92, Tianjin 300072, China.
authors.forename: Yakai
authors.identifier: http://orcid.org/0000-0002-4511-0874
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Institute of Biomaterials and Engineering, Wenzhou Medical University, Wenzhou, Zhejiang 325011, China and Wenzhou Institute of Biomaterials and Engineering, CNITECH, CAS, Wenzhou, Zhejiang 325011, China.
authors.forename: Changcan
authors.initials: C
authors.lastname: Shi
authors.affiliation: Department of Physiology and Pathophysiology, Logistics University of Chinese People's Armed Police Force, Tianjin 300162, China.
authors.forename: Wencheng
authors.initials: W
authors.lastname: Zhang
mesh_terms: D002460:Cell Line; D002465:Cell Movement; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D009025:Morpholines / Q000737:chemistry; D009842:Oligopeptides / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry*; D014157:Transcription Factors / Q000235:genetics*; D014162:Transfection / Q000379:methods*
chemical_list: D009025:Morpholines; D009842:Oligopeptides; D011091:Polyesters; D014157:Transcription Factors; C550122:ZNF580 protein, human; C520636:morpholine-2,5-dione; D011092:Polyethylene Glycols; C033616:poly(lactide); D011094:Polyethyleneimine