title: Developmental tracing of oocyte development in gonadal soma-derived factor deficiency medaka (Oryzias latipes) using a transgenic approach.
abstract: Gonadal soma-derived factor (gsdf) is reported to be a male initiator in medaka based on loss- and gain- of function via targeted disruption, or transgenic over-expression. However, little is known about how gsdf promotes undifferentiated gonad entry into male pathways or prevents entry into the female pathway. We utilized a visible folliculogenesis system with a reporter cassette of dual-color fluorescence expression to identify difference between oocyte development from wildtype and gsdf deficiency medaka. A red fluorescent protein (RFP) is driven by a major component of the synaptonemal complex (SYCP3) promoter which enables RFP expression solely in oocytes after the onset of meiosis, and a histone 2b-EGFP fused protein (H2BEGFP) under the control of an elongation factor (EF1α) promoter, wildly used as a mitotic reporter of cell cycle. This mitosis-meiosis visible switch revealed that early meiotic oocytes present in gsdf deficiency were more than those in wildtype ovaries, corresponding to the decrease of inhibin expression detected by real-time qPCR analysis, suggesting gsdf is tightly involved in the process of medaka oocyte development at early stage.
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China. Electronic address: gjguan@shou.edu.cn.
authors.forename: Guijun
authors.initials: G
authors.lastname: Guan
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Kaiqing
authors.initials: K
authors.lastname: Sun
authors.affiliation: Department of Biological Sciences, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Reproductive Endocrinology & Infertility, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Yanjiang Road 107, Guangdong 510120, China.
authors.forename: Xiaomiao
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Mingyou
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Biological Sciences, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Department of Biological Sciences, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore.
authors.forename: Yunzhi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Biological Sciences, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Chen
authors.affiliation: Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Zhuhai Key Laboratory of Marine Bioresources and Environment, School of Marine Sciences, Sun Yat-sen University, Guangzhou, China.
authors.forename: Meisheng
authors.initials: M
authors.lastname: Yi
authors.affiliation: Department of Biological Sciences, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore.
authors.forename: Yunhan
authors.initials: Y
authors.lastname: Hong
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian; D005260:Female; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006657:Histones / Q000235:genetics / Q000378:metabolism; D007265:Inhibins / Q000235:genetics* / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D008540:Meiosis; D008938:Mitosis; D009865:Oocytes / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D009990:Oryzias / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010053:Ovary / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D020648:Peptide Elongation Factor 1 / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013573:Synaptonemal Complex / Q000235:genetics / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000172:deficiency / Q000235:genetics*; D000097573:Red Fluorescent Protein
chemical_list: D029941:Fish Proteins; D049452:Green Fluorescent Proteins; D006657:Histones; D007265:Inhibins; D008164:Luminescent Proteins; D020648:Peptide Elongation Factor 1; D011993:Recombinant Fusion Proteins; D016212:Transforming Growth Factor beta; D000097573:Red Fluorescent Protein; C485184:enhanced green fluorescent protein
keywords: Folliculogenesis; Gonadal soma-derived factor (Gsdf); Meiosis; TGF-beta superfamily