title: miR-214-5p Targets ROCK1 and Suppresses Proliferation and Invasion of Human Osteosarcoma Cells.
abstract: MicroRNAs (miRNAs) are small conserved RNAs regulating specific target genes in posttranscriptional levels. They have been involved in multiple processes of tumor progression, including cell proliferation. miR-214-5p (also miR-214*) is a newly identified miRNA, and its functions are largely unknown. In this study, we explore the role of miR-214-5p in the proliferation and invasion of human osteosarcoma (OS) cells. The results showed that miR-214-5p was sharply reduced in OS tissues and cell lines, compared with normal tissues and cell lines. In addition, the miR-214-5p mimic greatly increased the miR-214-5p level and significantly decreased the proliferation and invasion of HOS and G293 OS cells. In contrast, the miR-214-5p inhibitor had a completely opposite effect on the miR-214-5p level, cell proliferation, and cell invasion. Moreover, bioinformatics and luciferase reporter gene assays confirmed that miR-1908 targeted the mRNA 3'-UTR region of ROCK1, a characterized tumor promoter in OS. In conclusion, miR-214-5p was identified as a new tumor suppressor, which directly targeted ROCK1 and suppressed proliferation of human OS cells.
authors.affiliation: Department of Orthopaedics, ChinaJapan Union Hospital, Jilin UniversityChangchunP.R. China.
authors.forename: Minglei
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedics, Si-ping Central HospitalSipingP.R. China.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, ChinaJapan Union Hospital, Jilin UniversityChangchunP.R. China.
authors.forename: Tongtong
authors.initials: T
authors.lastname: Zhu
authors.affiliation: Department of Orthopaedics, ChinaJapan Union Hospital, Jilin UniversityChangchunP.R. China.
authors.forename: Ruofeng
authors.initials: R
authors.lastname: Yin
mesh_terms: D020413:3' Untranslated Regions; D001483:Base Sequence; D001665:Binding Sites; D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology; D034622:RNA Interference; D054460:rho-Associated Kinases / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; C547397:MIRN214 microRNA, human; D035683:MicroRNAs; C515851:ROCK1 protein, human; D054460:rho-Associated Kinases