title: Zanthoxylumalkylamides ameliorate protein metabolism disorder in STZ-induced diabetic rats.
abstract: This study aimed to evaluate the protein metabolism effect ofZanthoxylumalkylamides and to explore the potential mechanism in streptozotocin (STZ)-induced diabetic rats. Diabetic rats were orally treated with 2, 4 and 8 mg per kg bw of alkylamides daily for 28 days. Alkylamides decreased the relative weight of the liver and food intake, significantly increased the relative skeletal muscle weight and significantly decreased the blood urea nitrogen levels. Insulin, insulin-like growth factor 1, total protein (TP) and albumin (ALB), globular proteins and ALB proteins/globulin protein levels in serum significantly increased. TP, RNA content and RNA/DNA ratio significantly increased in the skeletal muscle of diabetic rats. Real-time quantitative polymerase chain reaction results indicated that alkylamides significantly increased the mRNA expression of insulin receptor (InR), IGF1 and insulin-like growth factor 1 receptor (IGF1R) in the liver and skeletal muscle. Moreover, the mRNA and protein expression levels of PI3K, PKB and mTOR significantly increased, whereas those of atrogin-1, muscle ring finger 1 and FOXO in the skeletal muscle significantly decreased. Alkylamides may advance protein synthesis by the PI3K/PKB/mTOR signalling pathway and attenuate the catabolism of protein through the ubiquitin-proteasome pathway. Therefore, it was possible that alkylamides ameliorate protein metabolism disorders in diabetic rats by activating the mTOR pathway.
authors.affiliation: College of Food ScienceSouthwest University, Chongqing, China.|Laboratory of Quality & Safety Risk Assessment for Agro-products on Storage and Preservation (Chongqing)Ministry of Agriculture, Chongqing, China.
authors.forename: Tingyuan
authors.initials: T
authors.lastname: Ren
authors.affiliation: Institute of Biological EngineeringChongqing University, Chongqing, China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: College of Food ScienceSouthwest University, Chongqing, China.
authors.forename: Xuejuan
authors.initials: X
authors.lastname: Xia
authors.affiliation: College of Food ScienceSouthwest University, Chongqing, China.
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Department of NutritionDaping Hospital & Research Institute of Surgery, Third Military Medical University, Chongqing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Guo
authors.affiliation: College of Food ScienceSouthwest University, Chongqing, China.|Laboratory of Quality & Safety Risk Assessment for Agro-products on Storage and Preservation (Chongqing)Ministry of Agriculture, Chongqing, China.
authors.forename: Jianquan
authors.initials: J
authors.lastname: Kan
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism*; D004734:Energy Metabolism / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D053284:Polyunsaturated Alkamides / Q000737:chemistry / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects; D011506:Proteins / Q000378:metabolism*; D059748:Proteolysis / Q000187:drug effects; D051381:Rats; D011972:Receptor, Insulin / Q000235:genetics / Q000378:metabolism; D017451:Receptors, Somatomedin / Q000235:genetics / Q000378:metabolism; D032106:Zanthoxylum / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D053284:Polyunsaturated Alkamides; D011506:Proteins; D017451:Receptors, Somatomedin; D007334:Insulin-Like Growth Factor I; D011972:Receptor, Insulin
keywords: Zanthoxylum alkylamides; catabolism; diabetes; protein synthesis