title: Effects of Renal Ischemic Postconditioning on Myocardial Ultrastructural Organization and Myocardial Expression of Bcl-2/Bax in Rabbits.
abstract: We investigated the cardioprotective effect of renal ischemic postconditioning (RI-PostC) and its mechanisms in a rabbit model. Rabbits underwent 60 min of left anterior descending coronary artery occlusion (LADO) and 6 h of reperfusion. The ischemia-reperfusion (IR) group underwent LADO and reperfusion only. In the RI-PostC group, the left renal artery underwent 3 cycles of occlusion for 30 seconds and release for 30 seconds, before the coronary artery was reperfused. In the RI-PostC + GF109203X group, the rabbits received 0.05 mg/kg GF109203X (protein kinase C inhibitor) intravenously for 10 min followed by RI-PostC. Light microscopy and electron microscopy demonstrated that the RI-PostC group showed less pronounced changes, a smaller infarct region, and less apoptosis than the other two groups. Bcl-2 and Bax protein expression did not differ between the IR and RI-PostC + GF109203X groups. However, in the RI-PostC group, Bcl-2 protein expression was significantly higher and Bax protein expression was significantly lower than in the other two groups (P< 0.05). Changes in heart rate and mean arterial pressure were also smaller in the RI-PostC group than in the other two groups. These results indicate that RI-PostC can ameliorate myocardial ischemia-reperfusion injury and increase the Bcl-2/Bax ratio through a mechanism involving protein kinase C.
authors.affiliation: Department of Cardiology, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, China.
authors.forename: Wen-Zhong
authors.initials: WZ
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Li
authors.affiliation: Department of Cardiology, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, China.
authors.forename: Song
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Cardiology, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, China.
authors.forename: Ji-Dong
authors.identifier: 0000-0001-7993-6327
authors.initials: JD
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, China.
authors.forename: Xian-Feng
authors.initials: XF
authors.lastname: Ning
authors.affiliation: Department of Cardiology, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, China.
authors.forename: Shang-Lang
authors.initials: SL
authors.lastname: Cai
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D004195:Disease Models, Animal; D006801:Humans; D057775:Ischemic Postconditioning / Q000379:methods*; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D015428:Myocardial Reperfusion Injury / Q000235:genetics / Q000503:physiopathology / Q000628:therapy*; D009206:Myocardium / Q000473:pathology / Q000648:ultrastructure*; D011493:Protein Kinase C / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D011817:Rabbits; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D011493:Protein Kinase C