title: Wee1 and Cdc25 are controlled by conserved PP2A-dependent mechanisms in fission yeast.
abstract: Wee1 and Cdc25 are conserved regulators of mitosis. Wee1 is a kinase that delays mitosis via inhibitory phosphorylation of Cdk1, while Cdc25 is a phosphatase that promotes mitosis by removing the inhibitory phosphorylation. Although Wee1 and Cdc25 are conserved proteins, it has remained unclear whether their functions and regulation are conserved across diverse species. Here, we analyzed regulation of Wee1 and Cdc25 in fission yeast. Both proteins undergo dramatic cell cycle-dependent changes in phosphorylation that are dependent upon PP2A associated with the regulatory subunit Pab1. The mechanisms that control Wee1 and Cdc25 in fission yeast appear to share similarities to those in budding yeast and vertebrates, which suggests that there may be common mechanisms that control mitotic entry in all eukaryotic cells.
authors.affiliation: a Department of Molecular, Cell, and Developmental Biology , University of California , Santa Cruz, Santa Cruz , CA , USA.
authors.forename: Rafael
authors.initials: R
authors.lastname: Lucena
authors.affiliation: a Department of Molecular, Cell, and Developmental Biology , University of California , Santa Cruz, Santa Cruz , CA , USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Alcaide-Gavilán
authors.affiliation: a Department of Molecular, Cell, and Developmental Biology , University of California , Santa Cruz, Santa Cruz , CA , USA.
authors.forename: Steph D
authors.initials: SD
authors.lastname: Anastasia
authors.affiliation: a Department of Molecular, Cell, and Developmental Biology , University of California , Santa Cruz, Santa Cruz , CA , USA.
authors.forename: Douglas R
authors.initials: DR
authors.lastname: Kellogg
mesh_terms: D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000378:metabolism*; D017124:Conserved Sequence; D009687:Nuclear Proteins / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D010766:Phosphorylation; D054648:Protein Phosphatase 2 / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000166:cytology / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; D029702:Schizosaccharomyces pombe Proteins; C068689:wee1 protein, S pombe; D011505:Protein-Tyrosine Kinases; C570820:Cdc25 protein, S pombe; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2
keywords: Cdc25; Cell cycle; PP2APab1; Wee1; fission yeast