title: Survival, proliferation, and migration of human meningioma stem-like cells in a nanopeptide scaffold.
abstract: OBJECTIVE
  In order to grow cells in a three-dimensional (3D) microenvironment, self-assembling peptides, such as PuraMatrix, have emerged with potential to mimic the extracellular matrix. The aim of the present study was to investigate the influence of the self-assembling peptide on the morphology, survival, proliferation rate, migration potential, and differentiation of human meningioma stem-like cells (hMgSCs).
  
  
  METHODS
  The efficacy of a novel method for placing hMgSCs in PuraMatrix (the injection approach) was compared to the encapsulation and surface plating methods. In addition, we designed a new method for measurement of migration distance in 3D cultivation of hMgSCs in PuraMatrix.
  
  
  RESULTS
  Our results revealed that hMgSCs have the ability to form spheres in stem cell culture condition. These meningioma cells expressed GFAP, CD133, vimentin, and nestin. Using the injection method, a higher proliferation rate of the hMgSCs was observed after seven days of culture. Furthermore, the novel migration assay was able to measure the migration of a single cell alone in 3D environment.
  
  
  CONCLUSIONS
  The results indicate the injection method as an efficient technique for culturing hMgSCs in PuraMatrix. Furthermore, the novel migration assay enables us to evaluate the migration of hMgSCs.
authors.affiliation: Histology and Embryology group, Basic Science Department, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran; Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.
authors.forename: Sajad Sahab
authors.initials: SS
authors.lastname: Negah
authors.affiliation: Department of Neuroscience, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Hadi
authors.initials: H
authors.lastname: Aligholi
authors.affiliation: Histology and Embryology group, Basic Science Department, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran.
authors.forename: Zabihollah
authors.initials: Z
authors.lastname: Khaksar
authors.affiliation: Pediatric Department, Medical Faculty, Shahed University, Tehran, Iran.
authors.forename: Hadi
authors.initials: H
authors.lastname: Kazemi
authors.affiliation: Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.
authors.forename: Sayed Mostafa Modarres
authors.initials: SM
authors.lastname: Mousavi
authors.affiliation: Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran; Department of Community Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Safahani
authors.affiliation: Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.
authors.forename: Parastoo Barati
authors.initials: PB
authors.lastname: Dowom
authors.affiliation: Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran; Epilepsy Research Center, Westfälische Wilhelms-Universität Münster, Germany; Department of Neurology and Department of Neurosurgery, Westfälische Wilhelms-Universität Münster, Germany; Department of Neuroscience, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Gorji
keywords: Brain tumor; Cell culture; Cell migration; RADA16-I; Stem cells