title: High expression of Wls is associated with lymph node metastasis and advanced TNM stage in gastric carcinomas.
abstract: The roles of Wnt protein in carcinogenesis have been well documented in human cancers. Wls is a key modulator for the secretion of Wnt protein. We previously found that Wls was aberrantly expressed in colorectal carcinomas. Studies have revealed that dysregulation of Wnt signal transduction plays an important role in gastric carcinoma. We hypothesized that Wls may play a role in the development and progression of gastric carcinoma. In this study, three gastric cancer cell lines MGC-803, SGC-7901, and AGS, and a set of gastric carcinoma tissue specimens were subjected to immunohistochemistry. The relationship between the expression of Wls and clinicopathological parameters was analyzed. Wls was negatively detected in MGC-803, positively detected in SGC-7901 and AGS cell lines. Wls was weakly expressed in 9.7% (15/154), moderately in 33.1% (51/154), and strongly in 57.1% (88/154) of tested gastric carcinoma specimens. High expression of Wls was positively associated with well and moderately differentiated tumors (P = 0.035, rs= 0.170), lymph node metastasis (P = 0.001, rs= 0.276), and advanced TNM stage (P = 0.006, rs= 0.219). Our data suggest that Wls protein is related to tumor metastasis and advanced TNM stage, and may be used as a new marker for prognosis of gastric carcinoma.
authors.affiliation: Department of Pathology, Taixing People's Hospital, Taixing, Jiangshu 225400, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Hamamatsu University School of Medicine, 1-20-1, Handayama, Hamamatus 431-3192, Japan.
authors.forename: Hong
authors.initials: H
authors.lastname: Tao
authors.affiliation: Department of Pathology, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Pathology, Hamamatsu University School of Medicine, 1-20-1, Handayama, Hamamatus 431-3192, Japan.
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Sugimura
authors.affiliation: Department of Pathology, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China.
authors.forename: Jiandong
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Pathology, Taixing People's Hospital, Taixing, Jiangshu 225400, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins / Q000032:analysis / Q000096:biosynthesis*; D008207:Lymphatic Metastasis / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D043562:Receptors, G-Protein-Coupled / Q000032:analysis / Q000096:biosynthesis*; D013274:Stomach Neoplasms / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; D047908:Intracellular Signaling Peptides and Proteins; D043562:Receptors, G-Protein-Coupled; C549111:WLS protein, human
keywords: Wls; Wnt; gastric carcinoma; metastasis