title: Evidence of hemolysis in pigs infected with highly virulent African swine fever virus.
abstract: OBJECTIVE
  The research was conducted to understand more profoundly the pathogenetic aspects of the acute form of the African swine fever (ASF).
  
  
  METHODS
  A total of 10 pigs were inoculated with ASF virus (ASFV) (genotype II) in the study of the red blood cells (RBCs), blood and urine biochemistry in the dynamics of disease.
  
  
  RESULTS
  The major hematological differences observed in ASFV infected pigs were that the mean corpuscular volume, mean corpuscular hemoglobin, and hematocrits were significantly decreased compared to controls, and the levels of erythropoietin were significantly increased. Also were detected the trends of decrease in RBC count at terminal stages of ASF. Analysis of blood biochemistry revealed that during ASF development, besides bilirubinemia significantly elevated levels of lactate dehydrogenase, and aspartate aminotransferase were detected. Analysis of urine biochemistry revealed the presence of bilirubinuria, proteinuria during ASF development. Proteinuria, especially at late stages of the disease reflects a severe kidney damage possible glomerulonefritis.
  
  
  CONCLUSIONS
  The results of this study indicate the characteristics of developing hemolytic anemia observed in acute ASF (genotype II).
authors.affiliation: Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS RA, 7 Hasratyan Street, 0014 Yerevan, Armenia.
authors.forename: Zaven
authors.initials: Z
authors.lastname: Karalyan
authors.affiliation: Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS RA, 7 Hasratyan Street, 0014 Yerevan, Armenia.
authors.forename: Hovakim
authors.initials: H
authors.lastname: Zakaryan
authors.affiliation: Laboratory of Human Genomics and Immunomics, Institute of Molecular Biology of NAS RA, 7 Hasratyan Street, 0014 Yerevan, Armenia.
authors.forename: Elina
authors.initials: E
authors.lastname: Arakelova
authors.affiliation: Laboratory of Human Genomics and Immunomics, Institute of Molecular Biology of NAS RA, 7 Hasratyan Street, 0014 Yerevan, Armenia.
authors.forename: Violeta
authors.initials: V
authors.lastname: Aivazyan
authors.affiliation: Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS RA, 7 Hasratyan Street, 0014 Yerevan, Armenia.
authors.forename: Marina
authors.initials: M
authors.lastname: Tatoyan
authors.affiliation: Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS RA, 7 Hasratyan Street, 0014 Yerevan, Armenia.
authors.forename: Armen
authors.initials: A
authors.lastname: Kotsinyan
authors.affiliation: Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS RA, 7 Hasratyan Street, 0014 Yerevan, Armenia.
authors.forename: Roza
authors.initials: R
authors.lastname: Izmailyan
authors.affiliation: Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS RA, 7 Hasratyan Street, 0014 Yerevan, Armenia.
authors.forename: Elena
authors.initials: E
authors.lastname: Karalova
keywords: African swine fever virus; bilirubinemia; bilirubinuria; erythropoietin; hemolysis; proteinuria