title: Applying Human ADAR1p110 and ADAR1p150 for Site-Directed RNA Editing-G/C Substitution Stabilizes GuideRNAs against Editing.
abstract: Site-directed RNA editing is an approach to reprogram genetic information at the RNA level. We recently introduced a novel guideRNA that allows for the recruitment of human ADAR2 to manipulate genetic information. Here, we show that the current guideRNA design is already able to recruit another human deaminase, ADAR1, in both isoforms, p110 and p150. However, further optimization seems necessary as the current design is less efficient for ADAR1 isoforms. Furthermore, we describe hotspots at which the guideRNA itself is edited and show a way to circumvent this auto-editing without losing editing efficiency at the target. Both findings are important for the advancement of site-directed RNA editing as a tool in basic biology or as a platform for therapeutic editing.
authors.affiliation: Interfaculty Institute of Biochemistry, University of Tübingen, Auf der Morgenstelle 15, 72076 Tübingen, Germany. mad.heep@web.de.
authors.forename: Madeleine
authors.initials: M
authors.lastname: Heep
authors.affiliation: Interfaculty Institute of Biochemistry, University of Tübingen, Auf der Morgenstelle 15, 72076 Tübingen, Germany. pia.mach@student.uni-tuebingen.de.
authors.forename: Pia
authors.initials: P
authors.lastname: Mach
authors.affiliation: Interfaculty Institute of Biochemistry, University of Tübingen, Auf der Morgenstelle 15, 72076 Tübingen, Germany. philipp.reautschnig@uni-tuebingen.de.
authors.forename: Philipp
authors.initials: P
authors.lastname: Reautschnig
authors.affiliation: Interfaculty Institute of Biochemistry, University of Tübingen, Auf der Morgenstelle 15, 72076 Tübingen, Germany. jacqueline.wettengel@uni-tuebingen.de.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Wettengel
authors.affiliation: Interfaculty Institute of Biochemistry, University of Tübingen, Auf der Morgenstelle 15, 72076 Tübingen, Germany. thorsten.stafforst@uni-tuebingen.de.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Stafforst
keywords: ADAR; RNA repair; genetic disease; guideRNA; site-directed RNA editing