title: CD1-and CD1+porcine blood dendritic cells are enriched for the orthologues of the two major mammalian conventional subsets.
abstract: Conventional dendritic cells (cDC) are professional antigen-presenting cells that induce immune activation or tolerance. Two functionally specialised populations, termed cDC1 and cDC2, have been described in humans, mice, ruminants and recently in pigs. Pigs are an important biomedical model species and a key source of animal protein; therefore further understanding of their immune system will help underpin the development of disease prevention strategies. To characterise cDC populations in porcine blood, DC were enriched from PBMC by CD14 depletion and CD172a enrichment then stained with lineage mAbs (Lin; CD3, CD8α, CD14 and CD21) and mAbs specific for CD172a, CD1 and CD4. Two distinct porcine cDC subpopulations were FACSorted CD1-cDC (Lin-CD172+CD1-CD4-) and CD1+cDC (Lin-CD172a+CD1+CD4-), and characterised by phenotypic and functional analyses. CD1+cDC were distinct from CD1-cDC, expressing higher levels of CD172a, MHC class II and CD11b. Following TLR stimulation, CD1+cDC produced IL-8 and IL-10 while CD1-cDC secreted IFN-α, IL-12 and TNF-α. CD1-cDC were superior in stimulating allogeneic T cell responses and in cross-presenting viral antigens to CD8 T cells. Comparison of transcriptional profiles further suggested that the CD1-and CD1+populations were enriched for the orthologues of cDC1 and cDC2 subsets respectively.
authors.affiliation: Virology Department, Animal and Plant Health Agency, Addlestone, KT15 3NB, UK.
authors.forename: Jane C
authors.initials: JC
authors.lastname: Edwards
authors.affiliation: Virology Department, Animal and Plant Health Agency, Addlestone, KT15 3NB, UK.
authors.forename: Helen E
authors.initials: HE
authors.lastname: Everett
authors.affiliation: Virology Department, Animal and Plant Health Agency, Addlestone, KT15 3NB, UK.
authors.forename: Miriam
authors.initials: M
authors.lastname: Pedrera
authors.affiliation: Virology Department, Animal and Plant Health Agency, Addlestone, KT15 3NB, UK.
authors.forename: Helen
authors.initials: H
authors.lastname: Mokhtar
authors.affiliation: Nuffield Department of Medicine, University of Oxford, Oxford, OX1 3SY, UK.
authors.forename: Emanuele
authors.initials: E
authors.lastname: Marchi
authors.affiliation: Virology Department, Animal and Plant Health Agency, Addlestone, KT15 3NB, UK.
authors.forename: Ferran
authors.initials: F
authors.lastname: Soldevila
authors.affiliation: Flow Cytometry Facility, Animal and Plant Health Agency, Addlestone, KT15 3NB, UK.|Bacteriology Department, Animal and Plant Health Agency, Addlestone, KT15 3NB, UK.
authors.forename: Daryan A
authors.initials: DA
authors.lastname: Kaveh
authors.affiliation: Flow Cytometry Facility, Animal and Plant Health Agency, Addlestone, KT15 3NB, UK.|Bacteriology Department, Animal and Plant Health Agency, Addlestone, KT15 3NB, UK.
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Hogarth
authors.affiliation: Virology Department, Animal and Plant Health Agency, Addlestone, KT15 3NB, UK.
authors.forename: Helen L
authors.initials: HL
authors.lastname: Johns
authors.affiliation: Epidemiology Department, Animal and Plant Health Agency, Addlestone, KT15 3NB, UK.
authors.forename: Javier
authors.initials: J
authors.lastname: Nunez-Garcia
authors.affiliation: Virology Department, Animal and Plant Health Agency, Addlestone, KT15 3NB, UK.
authors.forename: Falko
authors.initials: F
authors.lastname: Steinbach
authors.affiliation: Virology Department, Animal and Plant Health Agency, Addlestone, KT15 3NB, UK.
authors.forename: Helen R
authors.initials: HR
authors.lastname: Crooke
authors.affiliation: Virology Department, Animal and Plant Health Agency, Addlestone, KT15 3NB, UK.
authors.forename: Simon P
authors.initials: SP
authors.lastname: Graham
mesh_terms: D000818:Animals; D018949:Antigens, CD1 / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis; D001773:Blood Cells / Q000737:chemistry* / Q000145:classification / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000737:chemistry* / Q000145:classification / Q000276:immunology*; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D013552:Swine; D013553:Swine Diseases
chemical_list: D018949:Antigens, CD1; D000954:Antigens, Surface; D016207:Cytokines