title: Adrenergic control of pinealocyte chondriome - an in vitro study.
abstract: Norepinephrine released from sympathetic innervation plays the main role in the regulation of melatonin secretion in mammalian pinealocytes. The present study was conducted for the following reasons: 1) to establish whether the pinealocyte chondriome is controlled by norepinephrine, 2) to determine the effect of adrenergic stimulation on mitochondria, and 3) to characterize adrenoceptors involved in the regulation of the chondriome. The static organ culture of the pineal gland was used. The explants were incubated for 5 consecutive days in control medium and between 20:00 and 08:00 in medium with the presence of 10 μM norepinephrine - adrenergic agonist; isoproterenol - beta-adrenoceptor agonist; cirazoline, methoxamine, M-6364 - alfa1 - adrenoceptors agonists or PMA - activator of PKC. The explants were then subjected to ultrastructural examination and morphometric analysis. The incubation of explants in the presence of norepinephrine or isoproterenol caused a decrease in the relative volume and the numerical density of mitochondria and induced an increase in the percentage of free mitochondria in pinealocytes. Significant changes in these parameters were not observed after treatment with methoxamine, cirazoline, M-6463 and PMA. The results obtained show that the chondriome of pig pinealocytes is controlled by norepinephrine acting via beta-adrenoceptors. Adrenergic stimulation, repeated for five consecutive days of organ culture, causes a decrease in the number of mitochondria and a shift in the distribution of mitochondria from the form of networks and filaments into the form of single particles. This indicates the intensive remodeling of the mitochondria network, which is closely linked to the metabolic status of the cell.
authors.forename: B
authors.initials: B
authors.lastname: Przybylska-Gornowicz
authors.forename: B
authors.initials: B
authors.lastname: Lewczuk
authors.forename: N
authors.initials: N
authors.lastname: Ziółkowska
authors.forename: M
authors.initials: M
authors.lastname: Prusik
mesh_terms: D058646:Adrenergic alpha-1 Receptor Agonists / Q000494:pharmacology; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D005260:Female; D007093:Imidazoles / Q000494:pharmacology*; D007545:Isoproterenol / Q000494:pharmacology*; D008729:Methoxamine / Q000494:pharmacology*; D009638:Norepinephrine / Q000494:pharmacology*; D010870:Pineal Gland / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D013552:Swine / Q000502:physiology*; D013566:Sympathomimetics / Q000494:pharmacology; D046509:Tissue Culture Techniques
chemical_list: D058646:Adrenergic alpha-1 Receptor Agonists; D000318:Adrenergic beta-Agonists; D007093:Imidazoles; D013566:Sympathomimetics; D008729:Methoxamine; D007545:Isoproterenol; C014282:cirazoline; D009638:Norepinephrine