title: OsCUL3a Negatively Regulates Cell Death and Immunity by Degrading OsNPR1 in Rice.
abstract: Cullin3-based RING E3 ubiquitin ligases (CRL3), composed of Cullin3 (CUL3), RBX1, and BTB proteins, are involved in plant immunity, but the function of CUL3 in the process is largely unknown. Here, we show that rice (Oryza sativa)OsCUL3ais important for the regulation of cell death and immunity. The rice lesion mimic mutantoscul3adisplays a significant increase in the accumulation of flg22- and chitin-induced reactive oxygen species, and in pathogenesis-related gene expression as well as resistance toMagnaporthe oryzaeandXanthomonas oryzaepvoryzae.We cloned theOsCUL3agene via a map-based strategy and found that the lesion mimic phenotype ofoscul3ais associated with the early termination of OsCUL3a protein. Interaction assays showed that OsCUL3a interacts with both OsRBX1a and OsRBX1b to form a multisubunit CRL in rice. Strikingly, OsCUL3a interacts with and degrades OsNPR1, which acts as a positive regulator of cell death in rice. Accumulation of OsNPR1 protein is greater in theoscul3amutant than in the wild type. Furthermore, theoscul3a osnpr1double mutant does not exhibit the lesion mimic phenotype of theoscul3amutant. Our data demonstrate that OsCUL3a negatively regulates cell death and immunity by degrading OsNPR1 in rice.
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311400 China.|State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.|Zhejiang Key Laboratory of Super Rice Research, China National Rice Research Institute, Hangzhou 311400, China.
authors.forename: Qunen
authors.identifier: 0000-0003-1190-7591
authors.initials: Q
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Yuese
authors.identifier: 0000-0003-1675-3114
authors.initials: Y
authors.lastname: Ning
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311400 China.|Zhejiang Key Laboratory of Super Rice Research, China National Rice Research Institute, Hangzhou 311400, China.
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311400 China.|Zhejiang Key Laboratory of Super Rice Research, China National Rice Research Institute, Hangzhou 311400, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311400 China.|Zhejiang Key Laboratory of Super Rice Research, China National Rice Research Institute, Hangzhou 311400, China.
authors.forename: Chunde
authors.initials: C
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311400 China.|Zhejiang Key Laboratory of Super Rice Research, China National Rice Research Institute, Hangzhou 311400, China.
authors.forename: Xiaodeng
authors.initials: X
authors.lastname: Zhan
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311400 China.|Zhejiang Key Laboratory of Super Rice Research, China National Rice Research Institute, Hangzhou 311400, China.
authors.forename: Weixun
authors.initials: W
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311400 China.|Zhejiang Key Laboratory of Super Rice Research, China National Rice Research Institute, Hangzhou 311400, China.
authors.forename: Daibo
authors.initials: D
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311400 China.
authors.forename: Xiangjin
authors.initials: X
authors.lastname: Wei
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China wang.620@osu.edu caolycgf@mail.hz.zj.cn shcheng@mail.hz.zj.cn.|Department of Plant Pathology, The Ohio State University, Columbus, Ohio 43210.
authors.forename: Guo-Liang
authors.identifier: 0000-0001-8877-3064
authors.initials: GL
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311400 China wang.620@osu.edu caolycgf@mail.hz.zj.cn shcheng@mail.hz.zj.cn.|Zhejiang Key Laboratory of Super Rice Research, China National Rice Research Institute, Hangzhou 311400, China.
authors.forename: Shihua
authors.initials: S
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311400 China wang.620@osu.edu caolycgf@mail.hz.zj.cn shcheng@mail.hz.zj.cn.|Zhejiang Key Laboratory of Super Rice Research, China National Rice Research Institute, Hangzhou 311400, China.|Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Liyong
authors.initials: L
authors.lastname: Cao
mesh_terms: D016923:Cell Death / Q000235:genetics; D003001:Cloning, Molecular; D055786:Gene Knockout Techniques; D012275:Oryza / Q000166:cytology / Q000235:genetics* / Q000276:immunology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism / Q000502:physiology; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D010940:Plant Proteins; D044767:Ubiquitin-Protein Ligases; D046988:Proteasome Endopeptidase Complex