title: Knocking down TCF8 inhibits high glucose- and angiotensin II-induced epithelial to mesenchymal transition in podocytes.
abstract: Epithelial to mesenchymal transition (EMT) is a physiological phenomenon in mammalian embryogenesis by which epithelial cells become mesenchymal stem cells. Studies have indicated that an inappropriate EMT plays a key role in a variety of pathogenic processes such as embryonic development and tumor metastasis. Moreover, recent studies have indicated EMT also plays an important role in renal fibrosis. In the current study, glucose and angiotensin II promoted EMT in podocytes as well as changes in the cellular morphology of podocytes. A high concentration of glucose and angiotensin II also promoted podocyte movement and migration. Moreover, a high concentration of glucose and angiotensin II promoted TCF8 expression. Inhibiting TCF8 expression with siRNA reversed EMT in podocytes in the presence of a high concentration of glucose and angiotensin. Inhibiting TCF8 expression also reversed changes in cellular morphology and podocyte movement and migration. Therefore, glucose and angiotensin II may promote EMT in podocytes via TCF8.
authors.affiliation: Department of Pharmacology, School of Pharmaceutical Sciences, Shandong University.
authors.forename: Changhuan
authors.initials: C
authors.lastname: Bai
authors.forename: Shumei
authors.initials: S
authors.lastname: Liang
authors.forename: Yunshan
authors.initials: Y
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Jiao
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D055785:Gene Knockdown Techniques; D020868:Gene Silencing / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology*; D050199:Podocytes / Q000187:drug effects* / Q000378:metabolism*; D000071799:Zinc Finger E-box-Binding Homeobox 1 / Q000172:deficiency* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D000071799:Zinc Finger E-box-Binding Homeobox 1; D000804:Angiotensin II; D005947:Glucose