title: Inhibition viral RNP and anti-inflammatory activity of coumarins against influenza virus.
abstract: Influenza viruses pose a severe threat to human health and a significant increase in antiviral drug-resistant among influenza viruses worldwide has been observed. Therefore, there is an urgent need to develop the new antiviral drugs, specifically from the natural products. In this study, the anti-viral and anti-inflammatory activities of coumarins against influenza A virus in vitro were investigated. One of the derivatives eleutheroside B1showed a wide spectrum of anti- human influenza virus effect with the IC50value of 64-125μg/ml in vitro, but it showed no effects against avian influenza virus. The time of addition was done and the results indicated that it had a potent antiviral effect when added at 0-6h, and also the virus yield was reduced by 60%. The influenza virus ribonucleoprotein was inhibited at 200μg/ml, and also the NP mRNA expression was inhibited at 50 and 200μg/ml. The expression level of cytokines and chemokines influenced by eleutheroside B1was further demonstrated, the IL-6, CXCL-8, CCL-2 expression were all inhibited by the eleuthe roside B1at concentration 200μg/ml. The findings of study suggest that eleutheroside B1can be as potential agent to develop for the prevention and treatment of influenza A virus.
authors.affiliation: State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120 PR China.
authors.forename: YuTao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Tropical Medicine, Guangzhou University of Chinese Medicine, Guangzhou, 510405 PR China.
authors.forename: Wen
authors.initials: W
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120 PR China.
authors.forename: QiaoLian
authors.initials: Q
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120 PR China.
authors.forename: WanYi
authors.initials: W
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120 PR China; State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa, Macau SAR, China. Electronic address: jeffyah@163.com.
authors.forename: Zifeng
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, 510006 PR China. Electronic address: mantianxing1997@126.com.
authors.forename: Xiong
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120 PR China. Electronic address: xinhuaw@gzhmu.edu.cn.
authors.forename: XinHua
authors.initials: X
authors.lastname: Wang
mesh_terms: D000072283:A549 Cells; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D045744:Cell Line, Tumor; D018932:Chemokine CCL2 / Q000378:metabolism; D054360:Chemokine CXCL1 / Q000378:metabolism; D003374:Coumarins / Q000494:pharmacology*; D004285:Dogs; D006801:Humans; D009980:Influenza A virus / Q000187:drug effects*; D007251:Influenza, Human / Q000188:drug therapy / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D061985:Madin Darby Canine Kidney Cells; D009976:Orthomyxoviridae Infections / Q000188:drug therapy / Q000378:metabolism; D012261:Ribonucleoproteins / Q000037:antagonists & inhibitors*
chemical_list: D000893:Anti-Inflammatory Agents; D000998:Antiviral Agents; D018932:Chemokine CCL2; D054360:Chemokine CXCL1; D003374:Coumarins; D015850:Interleukin-6; D012261:Ribonucleoproteins
keywords: Anti-inflammatory; Coumarins; Eleutheroside B(1); Influenza virus; Viral ribonucleoprotein