title: Induction, purification, and characterization of a thermo and pH stable laccase from Abortiporus biennis J2 and its application on the clarification of litchi juice.
abstract: A fungus J2 producing laccase with high yield was screened in soils and identified as Abortiporus biennis. The production of laccase was induced by 0.1 mM Cu2+, 0.1 mM tannic acid, and 0.5 M ethanol. The laccase from Abortiporus biennis J2 was purified to electrophoretic homogeneity by a couple of steps. The N-terminal amino acid sequence of the enzyme was AIGPTADLNISNADI. The properties of the purified laccase were investigated. The result showed the laccase from Abortiporus biennis J2 is a thermo and pH stable enzyme. The laccase activity was inhibited by Hg2+, Cd2+, Fe2+, Ag+, Cu2+, and Zn2+, while promoted by Mg2+, Mn2+at 10 mM level. Purified laccase was used to the clarification of litchi juice. After treatment with this laccase, the phenolic content of litchi juice had been found to be greatly reduced along with an increase in the clarity of the juice. The result indicated the potential of this laccase for application in juice procession.
authors.affiliation: a School of Life Science , South China Normal University , Guangzhou , China.
authors.forename: Liang
authors.initials: L
authors.lastname: Yin
authors.affiliation: a School of Life Science , South China Normal University , Guangzhou , China.
authors.forename: Jiayin
authors.initials: J
authors.lastname: Ye
authors.affiliation: a School of Life Science , South China Normal University , Guangzhou , China.
authors.forename: Sibi
authors.initials: S
authors.lastname: Kuang
authors.affiliation: a School of Life Science , South China Normal University , Guangzhou , China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Guan
authors.affiliation: a School of Life Science , South China Normal University , Guangzhou , China.
authors.forename: Rong
authors.initials: R
authors.lastname: You
mesh_terms: D000595:Amino Acid Sequence; D004790:Enzyme Induction; D004795:Enzyme Stability; D005511:Food Handling; D000067030:Fruit and Vegetable Juices; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D032125:Litchi / Q000737:chemistry*; D019216:Metals, Heavy / Q000494:pharmacology; D020072:Polyporales / Q000201:enzymology*; D013696:Temperature
chemical_list: D019216:Metals, Heavy; D042845:Laccase
keywords: Abortiporus biennis; clarification; laccase; litchi juice; purification