title: Photocontrolled extraction of uric acid from biological samples based on photoresponsive surface molecularly imprinted polymer microspheres.
abstract: We aim to develop novel photoresponsive surface molecularly imprinted polymer (SIMP) microspheres, an SiO2-SIMP, for the photocontrolled extraction of uric acid from biological samples. The SiO2-SMIP was prepared on silica microspheres by surface polymerization and characterized by using scanning electron microscopy, transmission electron microscopy, FTIR spectroscopy, thermogravimetric analysis, nitrogen adsorption-desorption analysis, and UV-visible spectroscopy. The SiO2-SMIP microspheres showed a photocontrolled uptake and release of uric acid in NaH2PO4buffer upon alternate irradiation at 365 and 440 nm. The SiO2-SMIP microspheres were able to photocontrollably extract uric acid from complicated biological samples for concentration analysis with no significant interference encountered and it exhibited very good recognition ability and fast binding kinetics toward uric acid.
authors.affiliation: The Key Laboratory of Applied Chemistry of Chongqing Municipality, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, China.
authors.forename: Cheng-Bin
authors.initials: CB
authors.lastname: Gong
authors.affiliation: The Key Laboratory of Applied Chemistry of Chongqing Municipality, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, China.
authors.forename: Zai-Yong
authors.initials: ZY
authors.lastname: Li
authors.affiliation: The Key Laboratory of Applied Chemistry of Chongqing Municipality, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, China.
authors.forename: Lan-Tao
authors.initials: LT
authors.lastname: Liu
authors.affiliation: The Key Laboratory of Applied Chemistry of Chongqing Municipality, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, China.
authors.forename: Yu-Bu
authors.initials: YB
authors.lastname: Wei
authors.affiliation: The Key Laboratory of Applied Chemistry of Chongqing Municipality, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Science and Environmental Studies, The Education University of Hong Kong, Tai Po Hong Kong SAR, China.
authors.forename: Cheuk-Fai
authors.initials: CF
authors.lastname: Chow
authors.affiliation: The Key Laboratory of Applied Chemistry of Chongqing Municipality, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Tang
mesh_terms: D000327:Adsorption; D006801:Humans; D008863:Microspheres; D054802:Molecular Imprinting; D011108:Polymers; D012822:Silicon Dioxide; D014527:Uric Acid / Q000302:isolation & purification* / Q000652:urine
chemical_list: D011108:Polymers; D014527:Uric Acid; D012822:Silicon Dioxide
keywords: molecular imprinting; photocontrolled extraction; surface molecularly imprinted polymers; uric acid; urine