title: Oral Delivery of Probiotics in Poultry Using pH-Sensitive Tablets.
abstract: As alternatives to antibiotics in livestocks, probiotics have been used, although most of them in the form of liquid or semisolid formulations, which show low cell viability after oral administration. Therefore, suitable dry dosage forms should be developed for livestocks to protect probiotics against the low pH in the stomach such that the products have higher probiotics survivability. Here, in order to develop a dry dosage forms of probiotics for poultry, we used hydroxypropyl methylcellulose phthalate 55 (HPMCP 55) as a tablet-forming matrix to develop probiotics in a tablet form for poultry. Here, we made three different kinds of probiotics-loaded tablet under different compression forces and investigated their characteristics based on their survivability, morphology, disintegration time, and kinetics in simulated gastrointestinal fluid. The results indicated that the probiotics formulated in the tablets displayed higher survival rates in acidic gastric conditions than probiotics in solution. Rapid release of the probiotics from the tablets occurred in simulated intestinal fluid because of fast swelling of the tablets in neutral pH. As a matrix of tablet, HPMCP 55 provided good viability of probiotics after 6 months under refrigeration. Moreover, after oral administration of probiotics-loaded tablets to chicken, more viable probiotics were observed, than with solution type, through several digestive areas of chicken by the tablets.
authors.affiliation: Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.|Laboratory of Translational Medicine, Chinese PLA General Hospital, Beijing 100853, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Hui-Shan
authors.initials: HS
authors.lastname: Li
authors.affiliation: Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Geon Goo
authors.initials: GG
authors.lastname: Han
authors.affiliation: Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Bijay
authors.initials: B
authors.lastname: Singh
authors.affiliation: Institute of Green-Bio Science and Technology, Seoul National University, Pyeongchang 25354, Republic of Korea.
authors.forename: Sang-Kee
authors.initials: SK
authors.lastname: Kang
authors.affiliation: Institute of Green-Bio Science and Technology, Seoul National University, Pyeongchang 25354, Republic of Korea.
authors.forename: Jin-Duck
authors.initials: JD
authors.lastname: Bok
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Dae-Duk
authors.initials: DD
authors.lastname: Kim
authors.affiliation: Department of Animal Science, Tianjin Agricultural University, Tianjin 300-384, P.R. China.
authors.forename: Zhong-Shan
authors.initials: ZS
authors.lastname: Hong
authors.affiliation: Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.|Department of Animal Science, Tianjin Agricultural University, Tianjin 300-384, P.R. China.
authors.forename: Yun-Jaie
authors.initials: YJ
authors.lastname: Choi
authors.affiliation: Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Chong-Su
authors.initials: CS
authors.lastname: Cho
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D015169:Colony Count, Microbial; D019245:Compressive Strength; D004339:Drug Compounding / Q000379:methods*; D004355:Drug Stability; D004356:Drug Storage; D005744:Gastric Acid / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007422:Intestines / Q000382:microbiology; D008747:Methylcellulose / Q000031:analogs & derivatives / Q000494:pharmacology; D050296:Microbial Viability; D011200:Poultry / Q000382:microbiology*; D019936:Probiotics / Q000008:administration & dosage* / Q000737:chemistry*; D013270:Stomach / Q000382:microbiology; D013607:Tablets / Q000737:chemistry* / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors
chemical_list: D013607:Tablets; D008747:Methylcellulose; C053309:hydroxypropyl methylcellulose phthalate
keywords: Probiotics; hydroxypropyl methylcellulose phthalate; oral delivery; pH-sensitive tablet; poultry