title: Molecular and epidemiological updates on cystic echinococcosis infecting water buffaloes from Egypt.
abstract: OBJECTIVE
  Cystic echinococcosis (CE) represents a serious parasitic disease at both animal and public health levels. The majority of reports negated the CE infection in buffaloes from Egypt; however, one study illustrated their infection with G6 genotype (camel strain). The present work contributed to update the epidemiological and molecular knowledge about CE infecting this economically important animal for better understanding of its role in maintaining theEchinococcuslife cycle.
  
  
  METHODS
  A total of 120 slaughtered water buffaloes at Mansoura abattoir, Dakahlia province, Egypt, were inspected for the existence of hydatid cysts. Cysts location and fertility were examined. Five out of 27 revealed cysts were tested molecularly using both cytochrome C oxidase subunit 1 and nicotinamide adenine dinucleotide hydrogen subunit 1 (nadh1) genes.
  
  
  RESULTS
  Low prevalence (4.2%) as well as considerably low fertility rate (14.8%) of buffaloes CE was noted. G1 genotype (common sheep strain) was revealed from the five examined cysts. At the level of nadh1 partial sequences, a globally singleton G1 haplotype was reported.
  
  
  CONCLUSIONS
  This the first report about the G1 infection in buffaloes from Egypt. This study proposed the minimized role of this animal in echinococcosis transmission. These findings could provide preliminary data for the local control of this disease.
authors.affiliation: Department of Parasitology, Faculty of Veterinary Medicine, Mansoura University, Egypt.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Abbas
keywords: Echinococcus; Egypt; buffalo; genotype; prevalence