title: Production of N-acyl homoserine lactones by the sponge-associated marine actinobacteria Salinispora arenicola and Salinispora pacifica.
abstract: The structures of acyl homoserine lactone (AHL) compounds and their quantification were accomplished using an integrated liquid chromatography-mass spectrometry approach. The precursor and product ions, along with retention times of peaks, were searched against an in-house database of AHLs and structures confirmed by accurate mass and by comparison with authentic AHL standards. The two compounds, N-(3-oxodecanoyl)-L-homoserine lactone and N-(3-oxododecanoyl)-L-homoserine lactone, were characterised and quantified in Salinispora sp. cultures.
authors.affiliation: School of Pharmacy, The University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Utpal
authors.initials: U
authors.lastname: Bose
authors.affiliation: Centre for Analytical Bioscience, School of Pharmacy, University of Nottingham, University Park, Nottingham NG7 2RD, UK.
authors.forename: Catharine A
authors.initials: CA
authors.lastname: Ortori
authors.affiliation: Centre for Analytical Bioscience, School of Pharmacy, University of Nottingham, University Park, Nottingham NG7 2RD, UK.
authors.forename: Sarir
authors.initials: S
authors.lastname: Sarmad
authors.affiliation: Centre for Analytical Bioscience, School of Pharmacy, University of Nottingham, University Park, Nottingham NG7 2RD, UK.
authors.forename: David A
authors.initials: DA
authors.lastname: Barrett
authors.affiliation: School of Pharmacy, The University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Amitha K
authors.initials: AK
authors.lastname: Hewavitharana
authors.affiliation: School of Pharmacy, The University of Queensland, Brisbane, QLD 4072, Australia.|Metabolomics Australia, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Mark P
authors.initials: MP
authors.lastname: Hodson
authors.affiliation: School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: John A
authors.initials: JA
authors.lastname: Fuerst
authors.affiliation: School of Pharmacy, The University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: P Nicholas
authors.initials: PN
authors.lastname: Shaw
authors.forename: Rich
authors.initials: R
authors.lastname: Boden
mesh_terms: D054742:Acyl-Butyrolactones / Q000032:analysis*; D000818:Animals; D059001:Aquatic Organisms / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002853:Chromatography, Liquid; D003470:Culture Media / Q000737:chemistry; D013058:Mass Spectrometry; D008848:Micromonosporaceae / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D011161:Porifera / Q000382:microbiology*
chemical_list: D054742:Acyl-Butyrolactones; D003470:Culture Media
keywords: homoserine lactone; liquid chromatography; marine bacteria; mass spectrometry