title: Iron Uptake Analysis in a Set of Clinical Isolates ofPseudomonas putida.
abstract: Pseudomonas putidastrains are frequent inhabitants of soil and aquatic niches and they are occasionally isolated from hospital environments. As the available iron sources in human tissues, edaphic, and aquatic niches are different, we have analyzed iron-uptake related genes in differentP. putidastrains that were isolated from all these environments. We found that these isolates can be grouped into different clades according to the genetics of siderophore biosynthesis and recycling. The pyoverdine locus of the sixP. putidaclinical isolates that have so far been completely sequenced, are not closely related; three strains (P. putidaHB13667, HB3267, and NBRC14164T) are grouped in Clade I and the other three in Clade II, suggesting possible different origins and evolution. In one clinical strain,P. putidaHB4184, the production of siderophores is induced under high osmolarity conditions. The pyoverdine locus in this strain is closely related to that of strainP. putidaHB001 which was isolated from sandy shore soil of the Yellow Sea in Korean marine sand, suggesting their possible origin, and evolution. The acquisition of two unique TonB-dependent transporters for xenosiderophore acquisition, similar to those existing in the opportunistic pathogenP. aeruginosaPAO, is an interesting adaptation trait of the clinical strainP. putidaH8234 that may confer adaptive advantages under low iron availability conditions.
authors.affiliation: Environmental Protection Department, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas Granada, Spain.
authors.forename: Lázaro
authors.initials: L
authors.lastname: Molina
authors.affiliation: Centre National de la Recherche Scientifique, UMR 7242, Université de Strasbourg, (ESBS) Illkirch, France.
authors.forename: Valérie A
authors.initials: VA
authors.lastname: Geoffroy
authors.affiliation: Environmental Protection Department, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas Granada, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Segura
authors.affiliation: Environmental Protection Department, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas Granada, Spain.
authors.forename: Zulema
authors.initials: Z
authors.lastname: Udaondo
authors.affiliation: Environmental Protection Department, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas Granada, Spain.
authors.forename: Juan-Luis
authors.initials: JL
authors.lastname: Ramos
keywords: Pseudomonas putida; clinical strains; iron; pyoverdine; siderophores