title: Effect of an MG132-Sustained Drug Delivery Capsular Ring on the Inhibition of Posterior Capsule Opacification in a Rabbit Model.
abstract: To design an MG132-sustained drug delivery capsular ring (SDDCR) and investigate its effect on the inhibition of posterior capsule opacification (PCO) in a rabbit model.
  
  
  
  The SDDCRs were prepared by forming a slice of film made by the mixture of poly lactic-co-glycolic acid (PLGA) and MG132 on the surface of capsular tension rings (CTRs). The drug-loading capacity, entrapment efficiency, and in vitro release of the drug-containing film were detected. Eighteen New Zealand white rabbits were operated with phacoemulsification and MG132-SDDCRs/PLGA-CTRs/CTRs implantation in the single eye. The images of the anterior segments were acquired at certain days, and the epithelial-mesenchymal transition (EMT) markers were detected by western blot and immunofluorescence.
  
  
  
  The drug-loading capacity and entrapment efficiency of MG132-SDDCRs were 1.15% ± 0.04% and 66.16% ± 0.027%, respectively, and the drug released well within a month. The PCO degree of the MG132-SDDCR group was significantly lower than the other groups. The expression of alpha-smooth muscle actin, fibronectin, vimentin, and collagen-I was lower, and the expression of E-cadherin (E-cad) was higher in the MG132-SDDCR group than the other groups.
  
  
  
  MG132-SDDCRs could be established successfully. The PCO process was prevented, and the expression of EMT markers was inhibited by the implantation of MG132-SDDCRs, indicating that this could be a potential treatment against PCO.
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University , Guangzhou, China .
authors.forename: Xuan
authors.initials: X
authors.lastname: Bao
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University , Guangzhou, China .
authors.forename: Min
authors.initials: M
authors.lastname: Hou
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University , Guangzhou, China .
authors.forename: Yingyan
authors.initials: Y
authors.lastname: Qin
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University , Guangzhou, China .
authors.forename: Furong
authors.initials: F
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University , Guangzhou, China .
authors.forename: Fu
authors.initials: F
authors.lastname: Shang
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University , Guangzhou, China .
authors.forename: Mingxing
authors.initials: M
authors.lastname: Wu
mesh_terms: D000818:Animals; D058442:Capsule Opacification / Q000188:drug therapy*; D004195:Disease Models, Animal; D016503:Drug Delivery Systems; D019344:Lactic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D007976:Leupeptins / Q000008:administration & dosage / Q000494:pharmacology*; D011100:Polyglycolic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D057852:Posterior Capsule of the Lens / Q000187:drug effects*; D011817:Rabbits
chemical_list: D007976:Leupeptins; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C072553:benzyloxycarbonylleucyl-leucyl-leucine aldehyde
keywords: MG132; capsular tension ring; epithelial–mesenchymal transition; posterior capsule opacification; sustained drug delivery system