title: Use of quantum dot beads-labeled monoclonal antibody to improve the sensitivity of a quantitative and simultaneous immunochromatographic assay for neuron specific enolase and carcinoembryonic antigen.
abstract: Detection of multiplex tumor markers was of great importance for cancer diagnosis. Immunochromatographic test strip (ICTS) was the most frequently-used point-of-care detection means. Herein, a convenient and fast method for simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA) was developed based on ICTS using quantum dot beads (QBs) as marking material. Good monodispersity, high colloidal stability and carboxyl-modified (COOH-) QBs were used. For this method, two test lines were applied to the NC membrane for simultaneous analysis of CEA and NSE respectively. The ideal limit of CEA and NSE detection was 0.0378ng/mL and 0.0426ng/mL with scarcely any cross-reactivity. Moreover, the fluorescent signal intensity of the nitrocellulose membrane could be easily read out in the cooperation of the "Handing" system without professional operators. The possible clinical utilization of this platform was demonstrated by detecting 100 clinic human serums. The result showed that the platform had sensitivity of 99% and 97% for CEA and NSE, while the specificity was 97% and 100% respectively. Our results indicated that the QBs based ICTS not only owning the ability of sensitive and specific simultaneous detection of CEA and NSE, but also showing the potential in developing this ICTS into a routine part of early lung cancer diagnosis.
authors.affiliation: Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: kun_x@foxmail.com.
authors.forename: Kun
authors.initials: K
authors.lastname: Xiao
authors.affiliation: Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China; Shanghai Engineering Research Center for Intelligent Diagnosis and Treatment Instrument, Shanghai 200240, China. Electronic address: wk_xa@163.com.
authors.forename: Kan
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: 1602701580@qq.com.
authors.forename: Weijian
authors.initials: W
authors.lastname: Qin
authors.affiliation: Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: yafeihou@sjtu.edu.cn.
authors.forename: Yafei
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Zhujiang Hospital, Southern Medical University, 253 Gongye Road, Guangzhou, Guangdong 510280, China. Electronic address: luwenting23@163.com.
authors.forename: Wenting
authors.initials: W
authors.lastname: Lu
authors.affiliation: School of Naval Architecture, Ocean & Civil Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: xudahao75@qq.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai 200011, China. Electronic address: woyansh@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wo
authors.affiliation: Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China; Shanghai Engineering Research Center for Intelligent Diagnosis and Treatment Instrument, Shanghai 200240, China. Electronic address: dxcui@sjtu.edu.cn.
authors.forename: Daxiang
authors.initials: D
authors.lastname: Cui
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002272:Carcinoembryonic Antigen / Q000032:analysis* / Q000097:blood; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D010751:Phosphopyruvate Hydratase / Q000032:analysis* / Q000097:blood; D019095:Point-of-Care Systems; D045663:Quantum Dots / Q000737:chemistry*; D013194:Staining and Labeling; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D010751:Phosphopyruvate Hydratase
keywords: Carcinoembryonic Antigen (CEA); Immunochromatographic test strip (ICTS); Neuron Specific Enolase (NSE); Quantum dot beads (QBs)