title: Simultaneous determination of amantadine, rimantadine, and memantine in processed products, chicken tissues, and eggs by liquid chromatography with tandem mass spectrometry.
abstract: A simultaneous determination of amantadine, rimantadine, and memantine in processed products (deep-fried chicken, fried chicken, fried quail egg, and grilled chicken) with liquid chromatography tandem mass spectrometry (LC-MS/MS) was developed. This new method was also applicable for chicken tissue (muscle, liver, and gizzard) and eggs. The chromatographic separation was performed on a Kinetex®XB-C18 core-shell technology column using a mobile phase of acetonitrile and 0.1% formic acid in a 10mmol/L ammonium formate solution, resulting in the complete separation of isomers (rimantadine and memantine) and any other obstructive peaks from the sample matrices. Sample preparation was performed by a modified QuEChERS method using acetonitrile and a 0.1% acetic acid extraction solution and cleaned using an Oasis®MCX cartridge. The sample matrix had no effect on the identification of the compounds. For quantification, an external solvent calibration curve was used. This new method exhibited good accuracy ranging from 79.9% to 91.5%. The relative standard deviation of repeatability (RSDr) ranged from 1.2% to 3.6% and the relative standard deviation of within-laboratory reproducibility (RSDWR) ranged from 1.3% to 6.0%. These standard deviations satisfied the criteria for Japanese validation guidelines. The limit of quantification (LOQ) was 1.0μg/kg for all samples. Analyte residues were not detected in 55 samples using the validated method.
authors.affiliation: Tokyo Metropolitan Institute of Public Health, Shinjuku-ku, Tokyo 169-0073, Japan. Electronic address: Yumi_Tsuruoka@member.metro.tokyo.jp.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Tsuruoka
authors.affiliation: Tokyo Metropolitan Institute of Public Health, Shinjuku-ku, Tokyo 169-0073, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nakajima
authors.affiliation: Tokyo Metropolitan Institute of Public Health, Shinjuku-ku, Tokyo 169-0073, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Kanda
authors.affiliation: Tokyo Metropolitan Institute of Public Health, Shinjuku-ku, Tokyo 169-0073, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hayashi
authors.affiliation: Tokyo Metropolitan Institute of Public Health, Shinjuku-ku, Tokyo 169-0073, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Matsushima
authors.affiliation: Tokyo Metropolitan Institute of Public Health, Shinjuku-ku, Tokyo 169-0073, Japan.
authors.forename: Souichi
authors.initials: S
authors.lastname: Yoshikawa
authors.affiliation: Tokyo Metropolitan Institute of Public Health, Shinjuku-ku, Tokyo 169-0073, Japan.
authors.forename: Marie
authors.initials: M
authors.lastname: Nagata
authors.affiliation: Tokyo Metropolitan Institute of Public Health, Shinjuku-ku, Tokyo 169-0073, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Koike
authors.affiliation: Tokyo Metropolitan Institute of Public Health, Shinjuku-ku, Tokyo 169-0073, Japan.
authors.forename: Chieko
authors.initials: C
authors.lastname: Nagano
authors.affiliation: Tokyo Metropolitan Institute of Public Health, Shinjuku-ku, Tokyo 169-0073, Japan.
authors.forename: Kotaro
authors.initials: K
authors.lastname: Sekimura
authors.affiliation: Tokyo Metropolitan Institute of Public Health, Shinjuku-ku, Tokyo 169-0073, Japan.
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Hashimoto
authors.affiliation: Tokyo Metropolitan Institute of Public Health, Shinjuku-ku, Tokyo 169-0073, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Takano
authors.affiliation: Tokyo Metropolitan Institute of Public Health, Shinjuku-ku, Tokyo 169-0073, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Shindo
mesh_terms: D000218:Adamantane / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D000818:Animals; D002645:Chickens; D002853:Chromatography, Liquid / Q000379:methods*; D004531:Eggs / Q000032:analysis*; D016014:Linear Models; D008460:Meat / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000218:Adamantane
keywords: Amantadine; External solvent calibration curve; LC–MS/MS; Memantine; Processed products; Rimantadine