title: Response of Lemna gibba L. to high and environmentally relevant concentrations of ibuprofen: Removal, metabolism and morpho-physiological traits for biomonitoring of emerging contaminants.
abstract: The increasing worldwide consumption of pharmaceuticals and personal care products such as ibuprofen (IBU) is leading to the widespread and persistent occurrence of these chemicals and their transformation products in soils and waters. Although at low concentrations, the continuous discharge of these micropollutants and the incomplete removal by the actual wastewater treatments can provoke accumulation in the environment with risks for the trophic chain. Non-target organisms as duckweed can be used for the environmental monitoring of pharmaceutical emerging contaminants. In this work, plants of Lemna gibba L. were exposed to high (0.20 and 1mgL-1) and environmentally relevant (0.02mgL-1) concentrations of IBU to investigate their removal and metabolization capacity. The main oxidized IBU metabolites in humans (hydroxy-IBU and carboxy-IBU) were determined in the intact plants and in the growth solutions, together with non-destructive physiological parameters and phytotoxic indicators. The IBU uptake increased with the increasing of IBU concentration in the medium, but the relative accumulation of the pharmaceutical and generation of hydroxy-IBU was higher in presence of the lower IBU treatments. Carboxy-IBU was not found in the plant tissue and solutions. The changes observed in growth and photosynthetic performances were not able to induce phyto-toxic effects. Apart from a mean physical-chemical degradation of 8.2%, the IBU removal by plants was highly efficient (89-92.5%) in all the conditions tested, highlighting the role of L. gibba in the biodegradation of emerging contaminants.
authors.affiliation: Institute of Agroenvironmental and Forest Biology, National Research Council, Monterotondo, RM, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Di Baccio
authors.affiliation: Institute of Agroenvironmental and Forest Biology, National Research Council, Monterotondo, RM, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Pietrini
authors.affiliation: Institute of Agroenvironmental and Forest Biology, National Research Council, Monterotondo, RM, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Bertolotto
authors.affiliation: Department of Environmental Chemistry, IDAEA-CSIC, Barcelona, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Pérez
authors.affiliation: Department of Environmental Chemistry, IDAEA-CSIC, Barcelona, Spain.
authors.forename: D
authors.initials: D
authors.lastname: Barcelò
authors.affiliation: Institute of Agroenvironmental and Forest Biology, National Research Council, Monterotondo, RM, Italy. Electronic address: massimo.zacchini@ibaf.cnr.it.
authors.forename: M
authors.initials: M
authors.lastname: Zacchini
authors.affiliation: Institute of Chemical Methodologies, National Research Council, Monterotondo, RM, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Donati
mesh_terms: D029064:Araceae / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004784:Environmental Monitoring; D007052:Ibuprofen / Q000032:analysis*; D062065:Wastewater; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D007052:Ibuprofen; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Biodegradation; Carboxyibuprofen; Chlorophyll fluorescence; Duckweed; Hydroxyibuprofen; Photosynthesis