title: Evaluation of methylglyoxal toxicity in human erythrocytes, leukocytes and platelets.
abstract: Methylglyoxal (MG) is a reactive dicarbonyl metabolite originated mainly from glucose degradation pathway that plays an important role in the pathogenesis of diabetes mellitus (DM). Reactions of MG with biological macromolecules (proteins, DNA and lipids) can induce cytotoxicity and apoptosis. Here, human erythrocytes, leukocytes and platelets were acutely exposed to MG at concentration ranging from 0.025 to 10 mM. Afterwards, hemolysis and osmotic fragility in erythrocytes, DNA damage and cell viability in leukocytes, and the activity of purinergic ecto-nucleotidases in platelets were evaluated. The levels of glycated products from leukocytes and free amino groups from erythrocytes and platelets were also measured. MG caused fragility of membrane, hemolysis and depletion of amino groups in erythrocytes. DNA damage, loss of cell viability and increased levels of glycated products were observed in leukocytes. In platelets, MG inhibited the activity of enzymes NTPDase, 5'-nucleotidase and adenosine deaminase (ADA) without affecting the levels of free amino groups. Our findings provide insights for understanding the mechanisms involved in MG acute toxicity towards distinct blood cells.
authors.affiliation: a Department of Biochemistry and Molecular Biology , Federal University of Santa Maria , Santa Maria , Brazil.
authors.forename: Alessandro de Souza
authors.initials: AS
authors.lastname: Prestes
authors.affiliation: a Department of Biochemistry and Molecular Biology , Federal University of Santa Maria , Santa Maria , Brazil.
authors.forename: Matheus Mülling
authors.initials: MM
authors.lastname: Dos Santos
authors.affiliation: a Department of Biochemistry and Molecular Biology , Federal University of Santa Maria , Santa Maria , Brazil.
authors.forename: Assis
authors.initials: A
authors.lastname: Ecker
authors.affiliation: a Department of Biochemistry and Molecular Biology , Federal University of Santa Maria , Santa Maria , Brazil.
authors.forename: Daniela
authors.initials: D
authors.lastname: Zanini
authors.affiliation: a Department of Biochemistry and Molecular Biology , Federal University of Santa Maria , Santa Maria , Brazil.
authors.forename: Maria Rosa Chitolina
authors.initials: MR
authors.lastname: Schetinger
authors.affiliation: a Department of Biochemistry and Molecular Biology , Federal University of Santa Maria , Santa Maria , Brazil.
authors.forename: Denis Broock
authors.initials: DB
authors.lastname: Rosemberg
authors.affiliation: a Department of Biochemistry and Molecular Biology , Federal University of Santa Maria , Santa Maria , Brazil.
authors.forename: João Batista Teixeira
authors.initials: JB
authors.lastname: da Rocha
authors.affiliation: a Department of Biochemistry and Molecular Biology , Federal University of Santa Maria , Santa Maria , Brazil.
authors.forename: Nilda Vargas
authors.initials: NV
authors.lastname: Barbosa
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism; D000243:Adenosine Deaminase / Q000378:metabolism; D000328:Adult; D001792:Blood Platelets / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D005260:Female; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D007962:Leukocytes / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D008297:Male; D009996:Osmotic Fragility / Q000187:drug effects; D011765:Pyruvaldehyde / Q000633:toxicity*
chemical_list: D011765:Pyruvaldehyde; D015720:5'-Nucleotidase; D000243:Adenosine Deaminase
keywords: Methylglyoxal; blood tissue; erythrocytes; leukocytes; platelets