title: Evaluation of organosolv pretreatment on the structural characteristics of lignin polymers and follow-up enzymatic hydrolysis of the substrates from Eucalyptus wood.
abstract: In the present study, Eucalyptus was subjected to organosolv pretreatment processes with aqueous 2-propanol at 200-220°C to obtain lignin with benign characters for its valorization and digestible substrates for bioethanol production. Results showed that different delignification ratios (64.00%-81.26%) and molecular weights (Mw=610-2680g/mol) of lignin fractions were dissociated from various pretreatment conditions, and the glucose yields of all the pretreated substrates significantly increased to 54.65-88.59% as compared to that of raw material (9.20%). Additionally, the amounts of β-O-4, β-β, and β-5 substructures were regularly decreased with the increased temperature and time. By contrast, the structural characteristics of the lignin fractions obtained with 70% 2-PrOH/water were less-altered than those with 50% 2-PrOH/water pretreatment. Remarkably, lignin with the lowest molecular weight (Mw=610g/mol) and less-altered chemical structure was achieved at 200°C for 120min, which is beneficial to subsequent catalytic degradation of lignin.
authors.affiliation: Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing, China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Shen
authors.affiliation: Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing, China. Electronic address: wenjialonghello@126.com.
authors.forename: Jia-Long
authors.initials: JL
authors.lastname: Wen
authors.affiliation: Shandong Longlive Bio-Technology Co., Ltd., Shandong, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing, China. Electronic address: rcsun3@bjfu.edu.cn.
authors.forename: Run-Cang
authors.initials: RC
authors.lastname: Sun
mesh_terms: D019840:2-Propanol / Q000737:chemistry*; D002480:Cellulase / Q000378:metabolism*; D005052:Eucalyptus / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry* / Q000302:isolation & purification; D012997:Solvents / Q000737:chemistry*; D014867:Water / Q000737:chemistry; D014934:Wood / Q000737:chemistry*
chemical_list: D012997:Solvents; D014867:Water; D008031:Lignin; D002480:Cellulase; D005947:Glucose; D019840:2-Propanol
keywords: 2-PrOH/water pretreatment; Enzymatic hydrolysis; Lignin structures