title: MicroRNA-200a Suppresses Cell Invasion and Migration by Directly Targeting GAB1 in Hepatocellular Carcinoma.
abstract: MicroRNA-200a (miR-200a) is frequently downregulated in most cancer types and plays an important role in carcinogenesis and cancer progression. In this study, we determined that miR-200a was downregulated in hepatocellular carcinoma (HCC) tissues and cell lines, consistent with the results of our previous study. Because a previous study suggested that downregulation of miR-200a is correlated with HCC metastasis, we aimed to elucidate the mechanism underlying the role of miR-200a in metastasis in HCC. Here we observed that overexpression of miR-200a resulted in suppression of HCC metastatic ability, including HCC cell migration, invasion, and metastasis, in vitro and in vivo. Furthermore, bioinformatics and luciferase reporter assays indicated that GAB1 is a direct target of miR-200a. Inhibition of GAB1 resulted in substantially decreased cell invasion and migration similar to that observed with overexpression of miR-200a in HCC cell lines, whereas restoration of GAB1 partially rescued the inhibitory effects of miR-200a. Taken together, these data provide novel information for comprehending the tumor-suppressive role of miR-200a in HCC pathogenesis through inhibition of GAB1 translation.
authors.affiliation: Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical UniversityXian, ShaanxiP.R. China.
authors.forename: Jianlin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical UniversityXian, ShaanxiP.R. China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Song
authors.affiliation: Department of Oncology, Tangdu Hospital, Fourth Military Medical UniversityXian, ShaanxiP.R. China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Shen
authors.affiliation: Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical UniversityXian, ShaanxiP.R. China.
authors.forename: Xisheng
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical UniversityXian, ShaanxiP.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.affiliation: Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical UniversityXian, ShaanxiP.R. China.
authors.forename: Sshibin
authors.initials: S
authors.lastname: Qu
authors.affiliation: Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical UniversityXian, ShaanxiP.R. China.
authors.forename: Runze
authors.initials: R
authors.lastname: Shang
authors.affiliation: Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical UniversityXian, ShaanxiP.R. China.
authors.forename: Ben
authors.initials: B
authors.lastname: Ma
authors.affiliation: Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical UniversityXian, ShaanxiP.R. China.
authors.forename: Meng
authors.initials: M
authors.lastname: Pu
authors.affiliation: Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical UniversityXian, ShaanxiP.R. China.
authors.forename: Kaishan
authors.initials: K
authors.lastname: Tao
authors.affiliation: Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical UniversityXian, ShaanxiP.R. China.
authors.forename: Kefeng
authors.initials: K
authors.lastname: Dou
authors.affiliation: Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical UniversityXian, ShaanxiP.R. China.
authors.forename: Haimin
authors.initials: H
authors.lastname: Li
mesh_terms: D020413:3' Untranslated Regions; D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000235:genetics*; D000818:Animals; D001665:Binding Sites; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D004195:Disease Models, Animal; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology*; D051379:Mice; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D009362:Neoplasm Metastasis; D034622:RNA Interference; D023041:Xenograft Model Antitumor Assays
chemical_list: D020413:3' Untranslated Regions; D048868:Adaptor Proteins, Signal Transducing; C098587:GAB1 protein, human; C533896:MIRN200 microRNA, human; D035683:MicroRNAs