title: Encapsulation of Sesbania grandiflora extract in polymeric micelles to enhance its solubility, stability, and antibacterial activity.
abstract: Clinical applications of Sesbania grandiflora bark extract (SGE) are limited because of its poor water solubility and stability. SGE was loaded in micelles of Pluronics. In vitro and in vivo antibacterial and toxicity tests were investigated using broth dilution and silkworm model. Aqueous solubility of SGE was improved by these micelles. Activity and toxicity of SGE loaded micelles were dependent on type and concentration of Pluronics. The micelles composed of 1:3 SGE to Pluronic F68 (SGE-PF68-13) showed small size (24.95 ± 0.34 nm), narrow PdI (<0.2), high entrapment efficiency (99.63 ± 0.19%) and negative zeta potential (-41.53 ± 0.15 mV). Stability of SGE in SGE-PF68-13 was 10 times higher than the unentrapped SGE. SGE-PF68-13 showed a dose dependent activity and significantly higher therapeutic effect than the unentrapped SGE. It is concluded that encapsulation of SGE in Pluronic micelles can enhance SGE solubility, stability, and antibacterial activity. SGE-PF68-13 is suitable for further study in mammalian animals.
authors.affiliation: a Department of Pharmaceutical Sciences, Faculty of Pharmacy , Chiang Mai University , Chiang Mai , Thailand.
authors.forename: Pimporn
authors.initials: P
authors.lastname: Anantaworasakul
authors.affiliation: a Department of Pharmaceutical Sciences, Faculty of Pharmacy , Chiang Mai University , Chiang Mai , Thailand.
authors.forename: Siriporn
authors.initials: S
authors.lastname: Okonogi
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use; D012831:Bombyx; D004337:Drug Carriers / Q000737:chemistry*; D004339:Drug Compounding; D006801:Humans; D008823:Micelles; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use; D020442:Poloxamer / Q000737:chemistry*; D049931:Sesbania / Q000737:chemistry*; D012995:Solubility; D013203:Staphylococcal Infections / Q000188:drug therapy*; D013211:Staphylococcus aureus / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D008823:Micelles; D010936:Plant Extracts; D020442:Poloxamer
keywords: Sesbania grandiflora; plant extract; poloxamer; silkworms; solubility; stability