title: Waste-to-biofuel: production of biobutanol from sago waste residues.
abstract: The main concern of extensive production of biobutanol has been associated with the high cost of the substrate and the relatively low tolerance of Clostridia to biobutanol production. In this study, the use of fermentable cassava waste residue (CWR) as substrate for biobutanol production was investigated using solvent-tolerant Clostridium sp. Four of obligatory, solvent-producing bacteria were isolated from sago industry waste sites. The NSW, PNAS1, SB5 and SBI4 strains showed identical profiles of 16S rRNA gene sequence similarity of Bacillus coagulans, Clostridium bifermentans and Clostridium sp. (97% similarity) and a wide range of carbohydrate substrate; however, the CWR was found to be suitable for the production of biobutanol considerably. Batch culture study was carried out using parameters such as time and temperature and carbon sources have been studied and optimized. Using pre-optimized CWR medium, significant amount of solvent production was observed in NSW, PNAS1, SB5 and SBI4 with 1.53, 3.36, 1.56 and 2.5 g L-1of butanol yield and 6.84, 9.012, 8.32 and 8.22 g L-1of total solvents, respectively. On the basis of these studies, NSW is proposed to represent the B. coagulans for butanol production directly from sago waste residues.
authors.affiliation: a Department of Biotechnology , School of Biosciences, Periyar University , Salem , Tamil Nadu , India.
authors.forename: Davidraj
authors.identifier: http://orcid.org/0000-0003-1561-661X
authors.initials: D
authors.lastname: Johnravindar
authors.affiliation: b Department of Environmental Science , Periyar University , Salem , Tamil Nadu , India.
authors.forename: Kumarasamy
authors.initials: K
authors.lastname: Murugesan
authors.affiliation: c Applied Research Centre for Pearl River Delta Environment, Department of Biology , Hong Kong Baptist University , Kowloon , Hong Kong.
authors.forename: Jonathan W C
authors.initials: JWC
authors.lastname: Wong
authors.affiliation: a Department of Biotechnology , School of Biosciences, Periyar University , Salem , Tamil Nadu , India.
authors.forename: Namasivayam
authors.initials: N
authors.lastname: Elangovan
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D056804:Biofuels; D000440:Butanols / Q000378:metabolism*; D007220:Industrial Waste; D002365:Manihot; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012997:Solvents / Q000378:metabolism*
chemical_list: D056804:Biofuels; D000440:Butanols; D007220:Industrial Waste; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012997:Solvents
keywords: ABE (Acetone–Butanol–Ethanol); Biobutanol; Cassava waste residues; Clostridium sp