title: Upregulated microRNA-143 inhibits cell proliferation in human nasopharyngeal carcinoma.
abstract: The aim of the present study was to investigate the possible functions and mechanism of microRNA (miR)-143 in cell proliferation of human nasopharyngeal carcinoma (NPC). The expression of miR-143 in NPC cells and tissues was investigated using reverse transcription-quantitative polymerase chain reaction. Cell viability assay, colony formation assay and flow cytometry were used to examine the cell proliferative ability and tumorigenicity. The expression levels of p21Cip1, p27Kip1, cyclin D1, phosphorylated (p)-retinoblastoma protein (Rb), Rb and cyclin-dependent kinase (CDK) 6 were determined by western blotting. Luciferase assay was used to confirm whether CDK6 was a direct target of miR-143. miR-143 was downregulated in NPC cell lines and tissues. Overexpression of miR-143 in NPC CNE1 cells inhibited proliferation, tumorigenicity and cell cycle progression. Additionally, ectopic expression of miR-143 downregulated cyclin D1 and p-Rb expression, and upregulated p21Cip1and p27Kip1expression, which subsequently inhibited NPC cell proliferation. It was also observed that CDK6 was a direct target of miR-143, which downregulated CDK6 expression. CDK6 suppression by miR-143 was associated with dysregulated expression of p21Cip1, p27Kip1, cyclin D1 and p-Rb, thereby serving an essential role in NPC cell growth. Our findings suggest that miR-143 inhibits proliferation by targeting CDK6, and may aid to identify new targets for anti-oncomiRs.
authors.affiliation: Department of Oncology, PLA421 Hospital, Guangzhou, Guangdong 510318, P.R. China.
authors.forename: Benfu
authors.initials: B
authors.lastname: He
authors.affiliation: Department of Pediatric Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Jinzhang
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Dayong
authors.initials: D
authors.lastname: Zheng
authors.affiliation: Department of Oncology, Integrated Chinese and Western Medicine Hospital, Southern Medical University, Guangzhou, Guangdong 510310, P.R. China.
authors.forename: Aimin
authors.initials: A
authors.lastname: Li
authors.affiliation: Department of Oncology, PLA458 Hospital, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Luo-Sheng
authors.initials: LS
authors.lastname: Zhang
keywords: CDK6; NPC; miR-143; proliferation