title: A DNA probe based on phosphorescent resonance energy transfer for detection of transgenic 35S promoter DNA.
abstract: A QDs-DNA nano-probe was made by combining Mn-doped ZnS room-temperature phosphorescence (RTP) quantum dots (QDs) and DNA. Then an RTP sensor for quantitative detection of genetically-modified mark sequence cauliflower mosaic virus 35S promoter (Ca MV 35S) DNA was built on basis of phosphorescent resonance energy transfer (PRET). The underlying principles were that a QDs-DNA water-soluble nano-probe was built by connecting single-strand DNA to the surfaces of QDs via a ligand exchange method. This probe had good RTP performance and could well identify Ca MV 35S. Thereby, the simple, rapid and efficient detection of genetically-modified organisms was realized. With the increase of target DNA sequence, the phosphorescent intensity of QDs was gradually reduced due to the energy transfer between QDs and the organic quencher BHQ2. This sensor had a detection limit of 4.03nM and a detection range of 12-300nM. Moreover, this sensor had high selectivity. This sensor could effectively detect the target DNA compared with mismatched and random sequences. Thus, this method is very promising for biological analysis.
authors.affiliation: Shanxi Normal University, Linfen 041004, PR China.
authors.forename: Jinzhi
authors.initials: J
authors.lastname: Lv
authors.affiliation: Shanxi Normal University, Linfen 041004, PR China.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Miao
authors.affiliation: Shanxi Normal University, Linfen 041004, PR China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Yang
authors.affiliation: Shanxi Normal University, Linfen 041004, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Qin
authors.affiliation: Shanxi Normal University, Linfen 041004, PR China.
authors.forename: Dongxia
authors.initials: D
authors.lastname: Li
authors.affiliation: Shanxi Normal University, Linfen 041004, PR China. Electronic address: gqyan2013@163.com.
authors.forename: Guiqin
authors.initials: G
authors.lastname: Yan
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D017796:Caulimovirus / Q000235:genetics*; D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D004735:Energy Transfer; D008163:Luminescent Measurements / Q000379:methods*; D011401:Promoter Regions, Genetic; D045663:Quantum Dots / Q000737:chemistry* / Q000648:ultrastructure; D013440:Sulfides / Q000737:chemistry; D019076:Transgenes; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: D015342:DNA Probes; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D013440:Sulfides; D017967:Zinc Compounds; C031238:zinc sulfide
keywords: DNA; Genetically-modified organisms; Phosphorescent resonance energy transfer; Room-temperature phosphorescence quantum dots