title: Neuroprotective effects of bone marrow-derived mesenchymal stem cellsand conditioned medium in mechanically injured neuroblastoma cells.
abstract: OBJECTIVE
  Bone marrow-derived mesenchymal stem cells (BMSCs) possess self-renewal characteristics that distinguish them from other cell types. Recent studies have focused on the effects of conditioned medium (CM) that includes the extracellular matrix. Here we examined the neuroprotective effects of BMSCs and CM on damaged neuroblastoma cells.
  
  
  METHODS
  The cells were divided into five groups: 1) healthy controls, 2) damaged cells alone, 3) damaged cells treated with BMSCs, 4) damaged cells treated with CM, and 5) damaged cells treated with both BMSCs and CM. Neuroprotective effects were then evaluated based upon the levels of oxidative stress, antitransforming growth factor β1 (anti-TGFβ1) production, and apoptosis.
  
  
  RESULTS
  Significant differences were observed between healthy controls and damaged cells (P < 0.001), as well as between damaged cells and those treated with BMSCs alone (P < 0.05), CM alone (P < 0.05), and both BMSCs and CM in combination (P < 0.01). Among the treated groups, the strongest neuroprotective effects were seen in cells treated with both BMSCs and CM.
  
  
  CONCLUSIONS
  These results show that both BMSCs and CM exhibit neuroprotective effects in damaged neuroblastoma cells. The strongest benefits were seen following treatment with both BMSCs and CM.
authors.affiliation: Department of Neurosurgery, Faculty of Medicine, Celal Bayar University, Manisa, Turkey.
authors.forename: Mesut
authors.initials: M
authors.lastname: Mete
authors.affiliation: Department Histology-Embryology, Faculty of Medicine, Celal Bayar University, Manisa, Turkey.
authors.forename: Işıl
authors.initials: I
authors.lastname: Aydemir
authors.affiliation: Department of Neurosurgery, Faculty of Medicine, Celal Bayar University, Manisa, Turkey.
authors.forename: Ülkün
authors.initials: Ü
authors.lastname: Ünlü Ünsal
authors.affiliation: Department of Neurosurgery, Faculty of Medicine, Celal Bayar University, Manisa, Turkey.
authors.forename: Yusuf Kurtuluş
authors.initials: YK
authors.lastname: Duransoy
authors.affiliation: Department Histology-Embryology, Faculty of Medicine, Celal Bayar University, Manisa, Turkey.
authors.forename: İbrahim Mehmet
authors.initials: İM
authors.lastname: Tuğlu
authors.affiliation: Department of Neurosurgery, Faculty of Medicine, Celal Bayar University, Manisa, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Selçuki
mesh_terms: D001853:Bone Marrow; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D059630:Mesenchymal Stem Cells; D009447:Neuroblastoma; D018696:Neuroprotective Agents
chemical_list: D017077:Culture Media, Conditioned; D018696:Neuroprotective Agents
keywords: Bone marrow derived mesenchymal stem cells; conditioned medium; injured; neuroblastoma cell; neuroprotective