title: Periodontal disease level-butyric acid putatively contributes to the ageing blood: A proposed link between periodontal diseases and the ageing process.
abstract: Periodontal diseases are partly attributable to periodontopathic bacteria found in the host, whereas, butyric acid (BA) is a common secondary metabolite produced by periodontopathic bacterial pathogens. BA has been linked to oxidative stress induction while oxidative stress has long been associated with the ageing process. However, the possible link between BA-induced oxidative stress and the ageing process has never been elucidated. Here, we attempted to show the possible role of periodontal diseaselevel-BA (PDL-BA) in influencing the rat blood ageing process. We injected PDL-BA into the young rat gingiva and, after 24h, heart blood extraction was performed. Blood obtained from PDL-BA-treated young rats was compared to untreated young and middle-aged rats. We found that cytosolic, but not mitochondrial, heme was affected 24h post-injection. In addition, we observed that PDL-BA treatment altered blood NOX activation, NADPH-related oxidative stress components (H2O2and GR), calcium homeostasis, cell death signals (CASP3 and CASP1), and age-related markers (SIRT1 and mTOR) in young rats, with some components more closely mimicking levels found in middle-aged rats. In this regard, we propose that PDL-BA may play a role in contributing to the rat blood ageing process.
authors.affiliation: Department of Microbiology, Nihon University School of Dentistry, Tokyo 101-8310 Japan. Electronic address: marni.cueno@nihon-u.ac.jp.
authors.forename: Marni E
authors.initials: ME
authors.lastname: Cueno
authors.affiliation: Department of Microbiology, Nihon University School of Dentistry, Tokyo 101-8310 Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Seki
authors.affiliation: Department of Microbiology, Nihon University School of Dentistry, Tokyo 101-8310 Japan.
authors.forename: Kuniyasu
authors.initials: K
authors.lastname: Ochiai
authors.affiliation: Department of Microbiology, Nihon University School of Dentistry, Tokyo 101-8310 Japan. Electronic address: imai.kenichi@nihon-u.ac.jp.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Imai
mesh_terms: D000375:Aging / Q000097:blood*; D000818:Animals; D015415:Biomarkers / Q000097:blood; D020148:Butyric Acid / Q000097:blood*; D016923:Cell Death; D005881:Gingiva / Q000378:metabolism* / Q000473:pathology; D008297:Male; D018384:Oxidative Stress; D010510:Periodontal Diseases / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015415:Biomarkers; D020148:Butyric Acid
keywords: Blood ageing; Butyric acid; Middle-aged rat; Periodontal disease; Young rat