title: Comparative pharmacokinetic study of the main components of cortex fraxini after oral administration in normal and hyperuricemic rats.
abstract: Cortex Fraxini is an important traditional Chinese herbal medicine used for the treatment of gout and hyperuricemia. An efficient and rapid ultra-performance liquid chromatography mass spectrometry method was developed and validated for simultaneous quantitation of six coumarins (aesculin, fraxin, aesculetin, fraxetin, sopoletin and 7-hydroxycoumarin) in normal and hyperuricemic rats plasma after oral administration of Cortex Fraxini. The method could successfully be applied for pharmacokinetics studies. The pharmacokinetic behavior of six coumarins in normal and hyperuricemia rats plasma was determined. Results showed that, for some of analytes, the pharmacokinetic parameters (AUC0-t, AUC0-∞, Cmax, Tmaxand CL) were significantly different between normal and hyperuricemic rats. The different pharmacokinetic parameters might result from renal impairment or a change of metabolic enzymes in the pathological state. The pharmacokinetic study in pathological state could provide more useful information to guide the clinical use of traditional Chinese herbal medicine.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, China.
authors.forename: Yinan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, China.
authors.forename: Min
authors.identifier: http://orcid.org/0000-0001-7214-8352
authors.initials: M
authors.lastname: Zhao
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Ye
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, China.
authors.forename: Yizhen
authors.initials: Y
authors.lastname: Shao
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, China.
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Yu
authors.affiliation: School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, China.
authors.forename: Chunjie
authors.initials: C
authors.lastname: Zhao
mesh_terms: D000284:Administration, Oral; D031319:Aesculus; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003374:Coumarins / Q000008:administration & dosage / Q000032:analysis* / Q000097:blood*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics*; D033461:Hyperuricemia / Q000188:drug therapy*; D057230:Limit of Detection; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D003374:Coumarins; D004365:Drugs, Chinese Herbal; C517354:horse chestnut seed
keywords: UPLC-MS/MS; cortex fraxini; coumarins; hyperuricemia; traditional Chinese medicine