title: Hepatitis E Virus Genotype 4 Sequences Detected in Sewage from Treatment Plants of China.
abstract: The aim of this study was to investigate the occurrence of hepatitis E virus (HEV) in sewage samples in Shen Zhen, China. Sewage samples were collected from 152 sewage plants including livestock sewage, domestic sewage and treated sewage from May to July of 2015. Two of 152 samples were HEV positive (1.32%) from the livestock sewage plants. Partial ORF2 fragments of HEV were sequenced and a phylogenetic tree was constructed using MEGA5.1. Blast and phylogenetic analyses showed that both of these two sequences belonged to HEV Genotype 4. To the best of our knowledge, this is the first study on the molecular characterization of HEV in wastewater in China and the first time to detect Genotype 4 in the sewage. Results from this study indicate that the possibilities of sporadic infections of HEV should be emphasized because virus still has the possibility to be circulating in the sewage in China.
authors.affiliation: Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Yuanmingyuan WestRoad 2#, Haidian District, Beijing, 100193, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.affiliation: Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Yuanmingyuan WestRoad 2#, Haidian District, Beijing, 100193, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Yuanmingyuan WestRoad 2#, Haidian District, Beijing, 100193, China. sheruiping@126.com.|Laboratory of Animal Pathology & Public Health, Key Laboratory of Zoonosis of the Ministry of Agriculture College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China. sheruiping@126.com.
authors.forename: Ruiping
authors.initials: R
authors.lastname: She
authors.affiliation: Shenzhen Urban Wastes Disposal & Recycling Center, Yuyuan Road 1#, Longgang District, Shenzhen, 518000, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Yu
authors.affiliation: Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Yuanmingyuan WestRoad 2#, Haidian District, Beijing, 100193, China.
authors.forename: Qiaoxing
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Yuanmingyuan WestRoad 2#, Haidian District, Beijing, 100193, China.
authors.forename: Jingling
authors.initials: J
authors.lastname: Yang
authors.affiliation: Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Yuanmingyuan WestRoad 2#, Haidian District, Beijing, 100193, China.
authors.forename: Fengjiao
authors.initials: F
authors.lastname: Hu
authors.affiliation: Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Yuanmingyuan WestRoad 2#, Haidian District, Beijing, 100193, China.
authors.forename: Majid Hussain
authors.initials: MH
authors.lastname: Soomro
authors.affiliation: Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Yuanmingyuan WestRoad 2#, Haidian District, Beijing, 100193, China.
authors.forename: Ruihan
authors.initials: R
authors.lastname: Shi
authors.affiliation: Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Yuanmingyuan WestRoad 2#, Haidian District, Beijing, 100193, China.
authors.forename: Wenzhuo
authors.initials: W
authors.lastname: Hao
authors.affiliation: Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Yuanmingyuan WestRoad 2#, Haidian District, Beijing, 100193, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Yuanmingyuan WestRoad 2#, Haidian District, Beijing, 100193, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Mao
mesh_terms: D000818:Animals; D002681:China; D005838:Genotype; D016752:Hepatitis E virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D058751:Livestock; D010802:Phylogeny; D012722:Sewage / Q000821:virology*; D062065:Wastewater / Q000821:virology
chemical_list: D012722:Sewage; D062065:Wastewater
keywords: Genotype 4; Hepatitis E virus; Urban sewage