title: Rapid methods for detection of bacterial resistance to antibiotics.
abstract: The most widely used antibiotic susceptibility testing methods in Clinical Microbiology are based on the phenotypic detection of antibiotic resistance by measuring bacterial growth in the presence of the antibiotic being tested. These conventional methods take typically 24hours to obtain results. Here we review the main techniques for rapid determination of antibiotic susceptibility. Data obtained with different methods such as molecular techniques, microarrays, commercial methods used in work routine, immunochromatographic methods, colorimetric methods, image methods, nephelometry, MALDI-TOF mass spectrometry, flow cytometry, chemiluminescence and bioluminescence, microfluids and methods based on cell disruption are analysed in detail.
authors.affiliation: Servicio de Microbiología e Inmunología, Hospital Clínico Universitario de Valladolid, Valladolid, España. Electronic address: gmr810@hotmail.com.
authors.forename: Gabriel Alberto
authors.initials: GA
authors.lastname: March-Rosselló
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents
keywords: Antibiograma directo; Antibiograma rápido; Direct antibiotic susceptibility test; Rapid antibiotic susceptibility test; Sensibilidad; Susceptibility