title: Nanohybrids consisting of magnetic nanoparticles and gold nanoclusters as effective peroxidase mimics and their application for colorimetric detection of glucose.
abstract: Although protein-stabilized gold nanoclusters (AuNCs) have gathered recent attention as biocompatible peroxidase mimics, their practical utility has been critically limited by the low catalytic activity. Here, the authors have developed a nanohybrid material to significantly enhance the catalytic activity of AuNCs by combining them with other inorganic enzyme mimetics, Fe3O4magnetic nanoparticles (MNPs), through electrostatic attraction. Owing to the synergistic effect by incorporating AuNCs and MNPs, the constructed nanohybrids yielded highly enhanced catalytic activity and enabled rapid catalytic oxidation of 3,3',5,5'-tetramethylbenzidine substrate to produce a blue-colored solution in proportional to the amount of H2O2. Moreover, a highly sensitive and selective glucose biosensing strategy was developed based on the coupled catalytic action between glucose oxidase and the nanohybrids. Using this method, target glucose was successfully detected in a linear concentration range from 150 to 750 μM with a detection limit as low as 100 μM. Along with excellent linearity, high precision and reproducibility were achieved by employing real human blood serums, which enables its use for the reliable quantification of glucose in practical use. Based on these results, the authors anticipate that the nanohybrids consisting of MNPs and AuNCs can serve as potent peroxidase mimics for the detection of clinically important target molecules.
authors.affiliation: Department of BioNano Technology, Gachon University, 1342 Seongnamdae-ro, Sujeong-gu, Seongnam, Gyeonggi 13120, South Korea.
authors.forename: Seongyeon
authors.initials: S
authors.lastname: Cho
authors.affiliation: Department of BioNano Technology, Gachon University, 1342 Seongnamdae-ro, Sujeong-gu, Seongnam, Gyeonggi 13120, South Korea.
authors.forename: Ho Yun
authors.initials: HY
authors.lastname: Shin
authors.affiliation: Department of BioNano Technology, Gachon University, 1342 Seongnamdae-ro, Sujeong-gu, Seongnam, Gyeonggi 13120, South Korea.
authors.forename: Moon Il
authors.initials: MI
authors.lastname: Kim
mesh_terms: D000327:Adsorption; D032701:Biomimetics; D015374:Biosensing Techniques / Q000379:methods; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005290:Ferric Compounds / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D009195:Peroxidase / Q000378:metabolism*; D015203:Reproducibility of Results; D055672:Static Electricity
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide; D006046:Gold; D009195:Peroxidase; D005947:Glucose