title: The effect of potassium diclofenac and dexamethasone on MMP-1 gene transcript levels during experimental tooth movement in rats.
abstract: OBJECTIVE
  The aim of this study was to analyze MMP-1 transcript levels in periodontal tissues of rats that underwent orthodontic treatment using potassium diclofenac and dexamethasone at different stages of tooth movement.
  
  
  METHODS
  The sample comprised of ninety male Wistar rats.
  
  
  METHODS
  A closed nickel-titanium coil spring was used to apply a force of 50 cN to move the maxillary right first molars mesially. One group received daily doses of 0.9% saline solution, the second group received daily doses of 5 mg/kg potassium diclofenac, and the third group received daily doses of 0.5 mg/kg dexamethasone. Tooth movement was observed on days 0, 1, 3, 7, and 14. MMP-1 transcript levels were evaluated by real-time polymerase chain reaction and the results were compared between groups by three-way ANOVA, with a significance level of 0.05.
  
  
  RESULTS
  Transcript levels increased in groups that received the coil spring treatment on all days of the experiment. MMP-1 expression was found to be decreased in groups treated with potassium diclofenac and dexamethasone compared to that in the control group, on days 1, 3, 5, and 7.
  
  
  CONCLUSIONS
  The application of orthodontic forces significantly increased MMP-1 transcript levels. The use of anti-inflammatory drugs may have an inhibitory effect on MMP-1 expression.
authors.affiliation: School of Life Sciences Curitiba, Pontifícia Universidade Católica do Paraná, Curitiba, Paraná, Brazil.
authors.forename: G P
authors.initials: GP
authors.lastname: Molina Da Silva
authors.affiliation: School of Life Sciences Curitiba, Pontifícia Universidade Católica do Paraná, Curitiba, Paraná, Brazil.
authors.forename: O M
authors.initials: OM
authors.lastname: Tanaka
authors.affiliation: School of Life Sciences Curitiba, Pontifícia Universidade Católica do Paraná, Curitiba, Paraná, Brazil.
authors.forename: D F
authors.initials: DF
authors.lastname: Campos Navarro
authors.affiliation: School of Dentistry of Bauru, Department of Biological Sciences, FOB/USP, Bauru, Brazil.
authors.forename: C E
authors.initials: CE
authors.lastname: Repeke
authors.affiliation: School of Dentistry of Bauru, Department of Biological Sciences, FOB/USP, Bauru, Brazil.
authors.forename: G P
authors.initials: GP
authors.lastname: Garlet
authors.affiliation: School of Life Sciences Curitiba, Pontifícia Universidade Católica do Paraná, Curitiba, Paraná, Brazil.
authors.forename: O
authors.initials: O
authors.lastname: Guariza-Filho
authors.affiliation: School of Life Sciences Curitiba, Pontifícia Universidade Católica do Paraná, Curitiba, Paraná, Brazil.
authors.forename: P C
authors.initials: PC
authors.lastname: Trevilatto
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology*; D004008:Diclofenac / Q000494:pharmacology*; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000187:drug effects* / Q000378:metabolism; D010519:Periodontium / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D017208:Rats, Wistar; D014087:Tooth Movement Techniques
chemical_list: D000893:Anti-Inflammatory Agents; D012333:RNA, Messenger; D004008:Diclofenac; D003907:Dexamethasone; D020781:Matrix Metalloproteinase 1
keywords: MMP-1; dexamethasone; orthodontic movement; potassium diclofenac