title: Optimal FSH usage in revascularization of allotransplanted ovarian tissue in mice.
abstract: BACKGROUND
  Ovarian transplantation is a useful method for preserving the fertility of young women with cancer who undergo radiotherapy and chemotherapy. Follicle-stimulating hormone (FSH) is use to protect transplanted ovarian tissues from ischemia injury through promoting revascularization after transplantation, but the side effect of high level FSH is ovarian overstimulation leading to substantial follicular loss. In this study, we investigated the optimal usage of FSH on revascularization in the in vitro cultured ovarian tissues before and after transplantation.
  
  
  RESULTS
  FSH mainly exhibited an additive response in the gene and protein expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and follicle stimulating hormone receptor (FSHR) with its raised concentrations (0.15 IU/ml, 0.30 IU/ml and 0.60 IU/ml) and prolonged treatment (3 h, 6 h, 12 h, 24 h). The concentrations with 0.60 IU/ml FSH could obviously promoted the expression of VEGF, bFGF and FSHR, but under this concentration FSH could also overstimulated the ovarian tissue leading to follicular loss. With the increase of culture time, the gene and protein expression of VEGF and bFGF both were up-regulated in all of the FSH added groups, but FSHR expression decreased when culture time exceeded 12 h. So we chose 0.30 IU/ml FSH added concentration and 6 h culture time as the FSH usage condition in functional revascularization verification experiment, and found that under this condition FSH promoted 2.5 times increase of vascular density in treated group than in control group after ovarian tissues transplantation.
  
  
  CONCLUSIONS
  Ovarian intervention with 0.30 IU/ml FSH for 6 h is an optimal FSH usage condition which could accelerate the revascularization in the allotransplanted ovarian tissue and can not produce ovarian overstimulation.
authors.affiliation: Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Key Laboratory of Reproduction and Genetic of Ningxia Hui Autonomous Region, and Department of Anatomy, Histology and Embryology, Ningxia Medical University, Shengli street No.1160, Yinchuan, 750004, China.
authors.forename: Wen-Zhi
authors.initials: WZ
authors.lastname: Ma
authors.affiliation: Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Key Laboratory of Reproduction and Genetic of Ningxia Hui Autonomous Region, and Department of Anatomy, Histology and Embryology, Ningxia Medical University, Shengli street No.1160, Yinchuan, 750004, China.
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Key Laboratory of Reproduction and Genetic of Ningxia Hui Autonomous Region, and Department of Anatomy, Histology and Embryology, Ningxia Medical University, Shengli street No.1160, Yinchuan, 750004, China.
authors.forename: Chang-Chun
authors.initials: CC
authors.lastname: Hei
authors.affiliation: Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Key Laboratory of Reproduction and Genetic of Ningxia Hui Autonomous Region, and Department of Anatomy, Histology and Embryology, Ningxia Medical University, Shengli street No.1160, Yinchuan, 750004, China.
authors.forename: Cheng-Jun
authors.initials: CJ
authors.lastname: Zhao
authors.affiliation: The No, 1 People's Hospital of xingtai, Hongxing street No.16, No, Xingtai, 054000, China.
authors.forename: Sha-Sha
authors.initials: SS
authors.lastname: Xie
authors.affiliation: Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Key Laboratory of Reproduction and Genetic of Ningxia Hui Autonomous Region, and Department of Anatomy, Histology and Embryology, Ningxia Medical University, Shengli street No.1160, Yinchuan, 750004, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Chang
authors.affiliation: Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Key Laboratory of Reproduction and Genetic of Ningxia Hui Autonomous Region, and Department of Anatomy, Histology and Embryology, Ningxia Medical University, Shengli street No.1160, Yinchuan, 750004, China.
authors.forename: Yu-Fang
authors.initials: YF
authors.lastname: Cai
authors.affiliation: Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Key Laboratory of Reproduction and Genetic of Ningxia Hui Autonomous Region, and Department of Anatomy, Histology and Embryology, Ningxia Medical University, Shengli street No.1160, Yinchuan, 750004, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Jia
authors.affiliation: Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Key Laboratory of Reproduction and Genetic of Ningxia Hui Autonomous Region, and Department of Anatomy, Histology and Embryology, Ningxia Medical University, Shengli street No.1160, Yinchuan, 750004, China. Peixiuying@163.com.
authors.forename: Xiu-Ying
authors.initials: XY
authors.lastname: Pei
authors.affiliation: Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Key Laboratory of Reproduction and Genetic of Ningxia Hui Autonomous Region, and Department of Anatomy, Histology and Embryology, Ningxia Medical University, Shengli street No.1160, Yinchuan, 750004, China. 4083304@163.com.
authors.forename: Yan-Rong
authors.initials: YR
authors.lastname: Wang
mesh_terms: D000818:Animals; D015415:Biomarkers; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D015870:Gene Expression; D007150:Immunohistochemistry; D051379:Mice; D018919:Neovascularization, Physiologic; D016377:Organ Transplantation; D010053:Ovary / Q000098:blood supply* / Q000378:metabolism / Q000637:transplantation*; D011962:Receptors, FSH / Q000235:genetics / Q000378:metabolism; D014184:Transplantation, Homologous; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D015415:Biomarkers; D011962:Receptors, FSH; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2; D005640:Follicle Stimulating Hormone
keywords: FSH; Ovarian tissue; Revascularization; VEGF; bFGF