title: Effects of human opiorphin on food intake and water intake in mice following central administration.
abstract: Human opiorphin plays an important pharmacological functions in rats or mice. The present study was performed to investigate effects and underlying mechanism of central injected opiorphin on food intake and water intake in mice. Intracerebroventricularly (i.c.v.) administered opiorphin (5-20μg/kg) dose-dependently suppressed food intake in fasted mice, but had no influence on food intake in freely feeding mice. The cumulative food intake was significantly decreased at 60min after injection of 10 and 20μg/kg opiorphin and the food intake was significantly reduced during the 20-60min period after treatment. Non-selected opiate receptor antagonist naloxone could fully block the inhibitory effect induced by opiorphin on cumulative food intake at 60min in fasted mice, suggesting that the anorexic effect of opiorphin was related to the opioid system. Moreover, the anorexic effect induced by opiorphin in fasted mice was also significantly inhibited by pretreatment with captopril or valsartan, which suggested that endogenous angiotensin may be involved in the response to opiorphin. Interestingly, the effect of opiorphin on water intake was increased in both fasted and freely feeding mice, which was completely blocked by captopril and valsartan. Furthermore, naloxone did not modify the effect of opiorphin on water intake. All together, the food and water intake effects of opiorphin may be due to the protection of the endogenous angiotensin and opioid peptides from degradation by NEP or APN.
authors.affiliation: Institute of Microbiology, School of Life Sciences, Lanzhou University, 222 Tian Shui South Road, Lanzhou, 730000, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Institute of Microbiology, School of Life Sciences, Lanzhou University, 222 Tian Shui South Road, Lanzhou, 730000, P.R. China.
authors.forename: Xiao-Zhu
authors.initials: XZ
authors.lastname: Tian
authors.affiliation: Institute of Microbiology, School of Life Sciences, Lanzhou University, 222 Tian Shui South Road, Lanzhou, 730000, P.R. China.
authors.forename: Lu
authors.initials: L
authors.lastname: Bai
authors.affiliation: Institute of Microbiology, School of Life Sciences, Lanzhou University, 222 Tian Shui South Road, Lanzhou, 730000, P.R. China.
authors.forename: Ze-Qi
authors.initials: ZQ
authors.lastname: Liu
authors.affiliation: Institute of Microbiology, School of Life Sciences, Lanzhou University, 222 Tian Shui South Road, Lanzhou, 730000, P.R. China.
authors.forename: Xing-Peng
authors.initials: XP
authors.lastname: Xiao
authors.affiliation: Institute of Microbiology, School of Life Sciences, Lanzhou University, 222 Tian Shui South Road, Lanzhou, 730000, P.R. China.
authors.forename: Pu
authors.initials: P
authors.lastname: Liu
authors.affiliation: Institute of Microbiology, School of Life Sciences, Lanzhou University, 222 Tian Shui South Road, Lanzhou, 730000, P.R. China. Electronic address: xkli@lzu.edu.cn.
authors.forename: Xiang-Kai
authors.initials: XK
authors.lastname: Li
mesh_terms: D047228:Angiotensin II Type 1 Receptor Blockers / Q000494:pharmacology; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology; D000818:Animals; D002216:Captopril / Q000494:pharmacology; D003624:Darkness; D004326:Drinking; D004435:Eating; D005215:Fasting; D006801:Humans; D007276:Injections, Intraventricular; D008297:Male; D051379:Mice; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D009842:Oligopeptides / Q000378:metabolism* / Q000494:pharmacology; D012084:Renin-Angiotensin System / Q000502:physiology; D012471:Salivary Proteins and Peptides / Q000378:metabolism* / Q000494:pharmacology; D000068756:Valsartan / Q000494:pharmacology
chemical_list: D047228:Angiotensin II Type 1 Receptor Blockers; D000806:Angiotensin-Converting Enzyme Inhibitors; D009292:Narcotic Antagonists; D009842:Oligopeptides; D012471:Salivary Proteins and Peptides; C517879:glutaminyl-arginyl-phenylalanyl-seryl-arginine; D009270:Naloxone; D000068756:Valsartan; D002216:Captopril
keywords: Anorexic effect; Food intake; Human opiorphin; Renin–angiotensin system; Water intake