title: Assessment of GAPDH expression by quantitative real time PCR in blood of Moroccan AD cases.
abstract: BACKGROUND
  Neuroproteomics studies have showed the high affinity interactions between GAPDH - β-amyloid in Alzheimer disease. The aim of our study is to complete our previous studies by assessing the mechanism responsible of decreased expression of GAPDH protein in the blood of Moroccan AD cases probably due to an alteration at the transcriptional level or at the post translational level.
  
  
  METHODS
  The mRNA expression of GAPDH was assessed by quantitative real time PCR in AD cases and healthy controls.
  
  
  RESULTS
  Our result revealed a significant difference of mRNA expression level of GAPDH in AD cases as compared to healthy controls (P<0.05).
  
  
  CONCLUSIONS
  This data is consistent with several studies by showing the direct involvement of GAPDH in amyloid aggregation by undergoing several modifications, which influence its chemical structure and its biological activity.
authors.affiliation: IBN ZOHR University, Faculté Polydisciplinaire de Taroudant, B.P: 271, 83 000 Taroudant, Morocco; Hassan II University of Casablanca, Laboratory of Medical Genetics and Molecular Pathology, Faculty of Medicine and Pharmacy, 19 Rue Tarik Ibnou Ziad, B.P: 9154, Morocco. Electronic address: elkadmiri1979@gmail.com.
authors.forename: Nadia
authors.initials: N
authors.lastname: El Kadmiri
authors.affiliation: Hassan II University of Casablanca, Laboratory of Medical Genetics and Molecular Pathology, Faculty of Medicine and Pharmacy, 19 Rue Tarik Ibnou Ziad, B.P: 9154, Morocco.
authors.forename: Meryam
authors.initials: M
authors.lastname: El Khachibi
authors.affiliation: Hassan II University of Casablanca, Laboratory of Medical Genetics and Molecular Pathology, Faculty of Medicine and Pharmacy, 19 Rue Tarik Ibnou Ziad, B.P: 9154, Morocco; Neurology Department, IBN ROCHD University, Hospital, rue des Hôpitaux, Casablanca, Morocco.
authors.forename: Ilham
authors.initials: I
authors.lastname: Slassi
authors.affiliation: Hassan II University of Casablanca, Laboratory of Medical Genetics and Molecular Pathology, Faculty of Medicine and Pharmacy, 19 Rue Tarik Ibnou Ziad, B.P: 9154, Morocco; Neurology Department, IBN ROCHD University, Hospital, rue des Hôpitaux, Casablanca, Morocco.
authors.forename: Bouchra
authors.initials: B
authors.lastname: El Moutawakil
authors.affiliation: Hassan II University of Casablanca, Laboratory of Medical Genetics and Molecular Pathology, Faculty of Medicine and Pharmacy, 19 Rue Tarik Ibnou Ziad, B.P: 9154, Morocco.
authors.forename: Sellama
authors.initials: S
authors.lastname: Nadifi
authors.affiliation: Laboratory of Physiology and Molecular Genetics, Faculty of Sciences Aïn Chock, Hassan II University, Casablanca, Morocco.
authors.forename: Abdelaziz
authors.initials: A
authors.lastname: Soukri
mesh_terms: D000544:Alzheimer Disease / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases
keywords: GAPDH; Moroccan AD cases; mRNA expression