title: Sclerostin Promotes Bone Remodeling in the Process of Tooth Movement.
abstract: Tooth movement is a biological process of bone remodeling induced by mechanical force. Sclerostin secreted by osteocytes is mechanosensory and important in bone remodeling. However, little is known regarding the role of sclerostin in tooth movement. In this study, models of experimental tooth movement were established in rats and mice. Sclerostin expression was investigated with immunohistochemistry staining, and osteoclastic activity was analyzed with tartrate-resistant acid phosphatase (TRAP) staining. MLO-Y4 osteocyte-like cells underwent uniaxial compression and tension stress or were cultured in hypoxia conditions. Expression of sclerostin was assessed by RT-qPCR and ELISA. MLO-Y4 cells were cultured with recombinant human sclerostin (rhSCL) interference and then co-cultured with RAW264.7 osteoclast precursor cells. Expressions of RANKL and OPG were analyzed by RT-qPCR, and osteoclastic activity was assessed by TRAP staining. During tooth movement, sclerostin was expressed differently in compression and tension sites. In SOST knock-out mice, there were significantly fewer TRAP-positive cells than in WT mice during tooth movement in compression sites. In-vitro studies showed that the expression of sclerostin in MLO-Y4 osteocyte-like cells was not different under a uniaxial compression and tension force, whereas hypoxia conditions significantly increased sclerostin expression in MLO-Y4 cells. rhSCL interference increased the expression of RANKL and the RANKL/OPG ratio in MLO-Y4 cells and the osteoclastic induction ability of MLO-Y4 cells in experimental osteocyte-osteoclast co-culture. These data suggest that sclerostin plays an important role in the bone remodeling of tooth movement.
authors.affiliation: Department of Orthodontics, State Key Laboratory of Oral Disease, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Shu
authors.affiliation: Department of Orthodontics, State Key Laboratory of Oral Disease, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Bai
authors.affiliation: School of Medicine and Dentistry, University of Rochester Medical Center, Rochester, New York, United States of America.
authors.forename: Tzongjen
authors.initials: T
authors.lastname: Sheu
authors.affiliation: Department of Orthodontics, State Key Laboratory of Oral Disease, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Orthodontics, State Key Laboratory of Oral Disease, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Xianrui
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Orthodontics, State Key Laboratory of Oral Disease, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Chaoran
authors.initials: C
authors.lastname: Xue
authors.affiliation: Department of Orthodontics, State Key Laboratory of Oral Disease, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Yiruo
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Orthodontics, State Key Laboratory of Oral Disease, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Mengyuan
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Department of Orthodontics, State Key Laboratory of Oral Disease, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Xianglong
authors.initials: X
authors.lastname: Han
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000378:metabolism* / Q000494:pharmacology; D016723:Bone Remodeling / Q000187:drug effects / Q000502:physiology*; D015687:Cell Hypoxia; D002460:Cell Line; D005819:Genetic Markers; D006023:Glycoproteins / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D010011:Osteocytes / Q000378:metabolism*; D053244:Osteoprotegerin / Q000378:metabolism; D053245:RANK Ligand / Q000378:metabolism; D000067996:RAW 264.7 Cells; D051381:Rats; D017207:Rats, Sprague-Dawley; D000071681:Tartrate-Resistant Acid Phosphatase / Q000378:metabolism; D014086:Tooth Mobility / Q000378:metabolism*; D014087:Tooth Movement Techniques
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D019485:Bone Morphogenetic Proteins; D005819:Genetic Markers; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D053244:Osteoprotegerin; D053245:RANK Ligand; C422903:SOST protein, human; C486030:Sost protein, mouse; C486031:Sost protein, rat; C506031:Tnfrsf11b protein, mouse; C506032:Tnfrsf11b protein, rat; C505689:Tnfsf11 protein, mouse; C000605510:ACP5 protein, human; C000605511:Acp5 protein, mouse; D000071681:Tartrate-Resistant Acid Phosphatase