title: Short-term in vivo exposure to graphene oxide can cause damage to the gut and testis.
abstract: Graphene oxide (GO) has unique physicochemical properties and also has a potentially widespread use in every field of daily life (industry, science, medicine). Demand for nanotechnology is growing every year, and therefore many aspects of its toxicity and biocompatibility still require further clarification. This research assesses the in vivo toxicity of pure and manganese ion-contaminated GO that were administrated to Acheta domesticus with food (at 200mgkg-1of food) throughout their ten-day adult life. Our results showed that short-term exposure to graphene oxide in food causes an increase in the parameters of oxidative stress of the tested insects (catalase - CAT, total antioxidant capacity - TAC), induces damage to the DNA at a level of approximately 35% and contributes to a disturbance in the stages of the cell cycle and causes an increase of apoptosis. Moreover, upon analyzing histological specimens, we found numerous degenerative changes in the cells of the gut and testis of Acheta domesticus as early as ten days after applying GO. A more complete picture of the GO risk can help to define its future applications and methods for working with the material, which may help us to avoid any adverse effects and damage to the animal.
authors.affiliation: Department of Animal Physiology and Ecotoxicology, University of Silesia in Katowice, Bankowa 9, PL 40-007 Katowice, Poland. Electronic address: mdziewiecka@us.edu.pl.
authors.forename: Marta
authors.initials: M
authors.lastname: Dziewięcka
authors.affiliation: Department of Animal Physiology and Ecotoxicology, University of Silesia in Katowice, Bankowa 9, PL 40-007 Katowice, Poland.
authors.forename: Julia
authors.initials: J
authors.lastname: Karpeta-Kaczmarek
authors.affiliation: Department of Animal Physiology and Ecotoxicology, University of Silesia in Katowice, Bankowa 9, PL 40-007 Katowice, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Augustyniak
authors.affiliation: Department of Animal Histology and Embryology, University of Silesia in Katowice, Bankowa 9, PL 40-007 Katowice, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Rost-Roszkowska
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D004249:DNA Damage; D005767:Gastrointestinal Diseases / Q000139:chemically induced* / Q000473:pathology; D006108:Graphite / Q000633:toxicity*; D006135:Gryllidae; D006801:Humans; D008274:Magnesium / Q000633:toxicity; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D013733:Testicular Diseases / Q000139:chemically induced* / Q000473:pathology
chemical_list: D000975:Antioxidants; D006108:Graphite; D002374:Catalase; D008274:Magnesium
keywords: Acheta domesticus; Gonads; Graphene oxide; Histology; Toxicity