title: A competitive ELISA for detection of group specific antibody to bluetongue virus using anti-core antibody.
abstract: Bluetongue virus (BTV) is transmitted by biting midges, which infects domestic and wild ruminants. In present study, a competitive enzyme-linked immunosorbent assay (C-ELISA) for the detection of serogroup-specific antibodies against VP7 protein of BTV has been developed. The assay measures the competition between a group specific antibody against core protein of BTV and a test serum to an optimized concentration of BTV recombinant-VP7 (r-VP7) antigen. Serum samples (n = 895) collected from small and large ruminants were used to optimize the C-ELISA. Percent inhibition (PI) values were used for estimation of the cut-off value for the C-ELISA. On receiver operator characteristic (ROC) analysis, different cut-off values along with their diagnostic sensitivity (DSn) and diagnostic specificity (DSp) were obtained. Among these, >50% PI value was accepted as cut-off at which DSn and Dsp was achieved as 97.6% and 98.0% respectively, at >95% confidence interval. Results show the present C-ELISA assay described to be sensitive, specific and reliable and could be adopted for serological investigation of small and large ruminants.
authors.affiliation: Division of Virology, ICAR-Indian Veterinary Research Institute, Mukteswar Campus, Dist. Nainital, 263 138, Uttarakhand, India. Electronic address: virusshield@gmail.com.
authors.forename: Karam
authors.initials: K
authors.lastname: Chand
authors.affiliation: Division of Virology, ICAR-Indian Veterinary Research Institute, Mukteswar Campus, Dist. Nainital, 263 138, Uttarakhand, India.
authors.forename: Sanchay K
authors.initials: SK
authors.lastname: Biswas
authors.affiliation: Division of Virology, ICAR-Indian Veterinary Research Institute, Mukteswar Campus, Dist. Nainital, 263 138, Uttarakhand, India.
authors.forename: Awadh B
authors.initials: AB
authors.lastname: Pandey
authors.affiliation: Division of Virology, ICAR-Indian Veterinary Research Institute, Mukteswar Campus, Dist. Nainital, 263 138, Uttarakhand, India.
authors.forename: Arpit
authors.initials: A
authors.lastname: Saxena
authors.affiliation: Division of Virology, ICAR-Indian Veterinary Research Institute, Mukteswar Campus, Dist. Nainital, 263 138, Uttarakhand, India.
authors.forename: Neha
authors.initials: N
authors.lastname: Tewari
authors.affiliation: Eastern Regional Station, ICAR-Indian Veterinary Research Institute, 37, Belgachia Road, Kolkata, 700 037, West Bengal, India.
authors.forename: Bimalendu
authors.initials: B
authors.lastname: Mondal
mesh_terms: D000820:Animal Diseases / Q000175:diagnosis / Q000276:immunology / Q000821:virology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D001819:Bluetongue / Q000097:blood / Q000276:immunology / Q000821:virology; D001820:Bluetongue virus / Q000276:immunology*; D002162:Camelus; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006041:Goats; D012372:ROC Curve; D011994:Recombinant Proteins / Q000276:immunology; D015203:Reproducibility of Results; D012756:Sheep; D014758:Viral Core Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D011994:Recombinant Proteins; D014758:Viral Core Proteins; C066862:VP7 protein, orbivirus
keywords: Bluetongue virus; C-ELISA; Serogroup; VP7 protein