title: Expression of chromosomal regional maintenance protein-1 may be associated with subcellular survivin expression in human gastric and colorectal carcinoma.
abstract: Survivin, a member of the inhibitor of apoptosis protein family, is a potential prognostic marker and molecular target for anticancer therapies. Chromosomal regional maintenance protein-1 (CRM-1) mediates the nuclear export of proteins such as survivin. The aims of the present study were to compare the expression and subcellular localization of CRM-1 in human gastric and colorectal carcinomas and to assess the association between CRM-1 and survivin expression in these tumor types. The nuclear and cytoplasmic CRM-1 expression rates in gastric carcinoma were 61% (42/69) and 29% (20/69), respectively, while the nuclear and cytoplasmic CRM-1 expression rates in colorectal carcinoma were 55% (43/78) and 37% (29/78), respectively. Nuclear and cytoplasmic CRM-1 expression was found to be significantly correlated with nuclear and cytoplasmic survivin expression in colorectal carcinoma, but not gastric carcinoma. These results indicate that CRM-1 expression patterns differ between gastric and colorectal carcinomas and thus, we hypothesize that CRM-1-mediated nuclear export of survivin may be deregulated in gastric carcinoma. Therefore, CRM-1 may exhibit different functions in gastric and colorectal carcinoma.
authors.affiliation: Laboratory of Pathology, Division of Medical Biophysics, Kobe University Graduate School of Health Sciences, Kobe, Hyogo 654-0142, Japan.
authors.forename: Michiko
authors.initials: M
authors.lastname: Shintani
authors.affiliation: Laboratory of Pathology, Division of Medical Biophysics, Kobe University Graduate School of Health Sciences, Kobe, Hyogo 654-0142, Japan.
authors.forename: Akito
authors.initials: A
authors.lastname: Tashiro
authors.affiliation: Department of Diagnostic Pathology, Osaka Red Cross Hospital, Osaka 543-8555, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Sangawa
authors.affiliation: Department of Clinical Laboratory, Kuma Hospital, Kobe, Hyogo 650-0011, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamao
authors.affiliation: Laboratory of Pathology, Division of Medical Biophysics, Kobe University Graduate School of Health Sciences, Kobe, Hyogo 654-0142, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Kamoshida
keywords: chromosomal regional maintenance protein-1; colorectal carcinoma; gastric carcinoma; immunohistochemistry; survivin