title: Antioxidant and Anti-Osteoporotic Activities of Aromatic Compounds and Sterols from Hericium erinaceum.
abstract: Hericium erinaceum, commonly called lion's mane mushroom, is a traditional edible mushroom widely used in culinary applications and herbal medicines in East Asian countries. In this study, a new sterol, cerevisterol 6-cinnamate (6), was isolated from the fruiting bodies ofH. erinaceumtogether with five aromatic compounds1-5and five sterols7-11. The chemical structures of these compounds were elucidated using chemical and physical methods and comparison of HRESIMS, ¹D-NMR (¹H,13C, and DEPT) and 2D-NMR (COSY, HMQC, HMBC, and NOESY) spectra with previously reported data. The antioxidant and anti-osteoporotic activities of extracts and the isolated compounds1-11were investigated. All compounds exhibited peroxyl radical-scavenging capacity but only compounds1,3, and4showed potent reducing capacity. Moreover, compounds1,2,4, and5showed moderate effects on cellular antioxidant activity and inhibited the receptor activator of nuclear factor κB ligand (RANKL)-induced osteoclastic differentiation. These results suggested thatH. erinaceumcould be utilized in the development of natural antioxidant and anti-osteoporotic nutraceuticals and functional foods.
authors.affiliation: Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, Daegu 41062, Korea. liwei1986@kiom.re.kr.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Food and Nutrition, Hannam University, Daejeon 34430, Korea. shlee@hnu.ac.kr.
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Food and Nutrition, Hannam University, Daejeon 34430, Korea. haedong@hnu.ac.kr.
authors.forename: Hae Dong
authors.initials: HD
authors.lastname: Jang
authors.affiliation: Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, Daegu 41062, Korea. jyma@kiom.re.kr.
authors.forename: Jin Yeul
authors.initials: JY
authors.lastname: Ma
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon 34134, Korea. yhk@cnu.ac.kr.
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000578:Amidines / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001487:Basidiomycota / Q000737:chemistry*; D050071:Bone Density Conservation Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002934:Cinnamates / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D055951:Functional Food / Q000032:analysis; D015870:Gene Expression; D056945:Hep G2 Cells; D006801:Humans; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D015394:Molecular Structure; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016877:Oxidants / Q000037:antagonists & inhibitors / Q000494:pharmacology; D010545:Peroxides / Q000037:antagonists & inhibitors; D010840:Phytosterols / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D053245:RANK Ligand / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D000071681:Tartrate-Resistant Acid Phosphatase / Q000235:genetics / Q000378:metabolism
chemical_list: D000578:Amidines; D000975:Antioxidants; D050071:Bone Density Conservation Agents; D002934:Cinnamates; D016877:Oxidants; D010545:Peroxides; D010840:Phytosterols; D053245:RANK Ligand; C505689:Tnfsf11 protein, mouse; C049375:perhydroxyl radical; C006954:cerevisterol; C046728:2,2'-azobis(2-amidinopropane); C000605511:Acp5 protein, mouse; D000071681:Tartrate-Resistant Acid Phosphatase
keywords: Hericium erinaceum; anti-osteoporosis; antioxidant; aromatic compound; hericiaceae; sterol