title: Middle age enhances expression of innate immunity genes in a female mouse model of pulmonary fibrosis.
abstract: The lungs are highly sensitive to tissue fibrosis, with a clear age-related component. Among the possible triggers of pulmonary fibrosis are repeated inhalations of fine organic particles. How age affects this response, is still far from being fully understood. We examined the impact of middle-age on gene expression in pulmonary fibrosis, using the novel "inhalation challenge set" mouse model. Our results demonstrate that the response of female mice to exposure of Pantoea agglomerans extract primarily involves various immune-related pathways and cell-cell/cell-extracellular matrix interactions. We found that middle-age had a strong effect on the response to the P. agglomerans-induced lung fibrosis, featured by a more rapid response and increased magnitude of expression changes. Genes belonging to innate immunity pathways (such as the TLR signaling and the NK-cell mediated cytotoxicity) were particularly up-regulated in middle-aged animals, suggesting that they may be potential targets for the treatment of pulmonary fibrosis caused by inhalations of organic particles. Our analysis also highlights the relevance of the "inhalation challenge set" mouse model to lung aging and related pathology.
authors.affiliation: Institute of Rural Health, Jaczewskiego 2, 20-090, Lublin, Poland. msgolec@yahoo.com.
authors.forename: Marcin
authors.identifier: 0000-0001-9115-6358
authors.initials: M
authors.lastname: Golec
authors.affiliation: AIT - Austrian Institute of Technology, Vienna, Austria.
authors.forename: Matthias
authors.initials: M
authors.lastname: Wielscher
authors.affiliation: Institute of Rural Health, Jaczewskiego 2, 20-090, Lublin, Poland.
authors.forename: Marta Kinga
authors.initials: MK
authors.lastname: Lemieszek
authors.affiliation: AIT - Austrian Institute of Technology, Vienna, Austria.
authors.forename: Klemens
authors.initials: K
authors.lastname: Vierlinger
authors.affiliation: Institute of Rural Health, Jaczewskiego 2, 20-090, Lublin, Poland.
authors.forename: Czesława
authors.initials: C
authors.lastname: Skórska
authors.affiliation: AIT - Austrian Institute of Technology, Vienna, Austria.
authors.forename: Sophia
authors.initials: S
authors.lastname: Huetter
authors.affiliation: Institute of Rural Health, Jaczewskiego 2, 20-090, Lublin, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Sitkowska
authors.affiliation: Department of Pneumonology, Oncology and Allergology, Medical University of Lublin, Lublin, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Mackiewicz
authors.affiliation: Institute of Rural Health, Jaczewskiego 2, 20-090, Lublin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Góra-Florek
authors.affiliation: Department of Internal Medicine II, Clinical Division of Pulmonary Medicine, Medical University of Vienna, Vienna, Austria.
authors.forename: Rolf
authors.initials: R
authors.lastname: Ziesche
authors.affiliation: The Shraga Segal Department of Microbiology, Immunology and Genetics, Center for Multidisciplinary Research on Aging, Ben-Gurion University of the Negev, Beer Sheva, Israel.
authors.forename: Hagai
authors.initials: H
authors.lastname: Yanai
authors.affiliation: The Shraga Segal Department of Microbiology, Immunology and Genetics, Center for Multidisciplinary Research on Aging, Ben-Gurion University of the Negev, Beer Sheva, Israel.
authors.forename: Vadim E
authors.initials: VE
authors.lastname: Fraifeld
authors.affiliation: Institute of Rural Health, Jaczewskiego 2, 20-090, Lublin, Poland.|Department of Pneumonology, Oncology and Allergology, Medical University of Lublin, Lublin, Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Milanowski
authors.affiliation: Institute of Rural Health, Jaczewskiego 2, 20-090, Lublin, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Dutkiewicz
mesh_terms: D000375:Aging / Q000276:immunology*; D000818:Animals; D016207:Cytokines / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D007113:Immunity, Innate / Q000276:immunology*; D007155:Immunologic Factors / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D020636:Pantoea / Q000276:immunology; D011658:Pulmonary Fibrosis / Q000276:immunology*
chemical_list: D016207:Cytokines; D007155:Immunologic Factors
keywords: Aging; Gene expression; Hypersensitivity pneumonitis; Mice; Pulmonary fibrosis