title: Anti-Inflammatory and Antibothropic Properties ofJatropha Elliptica, a Plant from Brazilian Cerrado Biome.
abstract: Purpose:The aim of this study was to evaluate the antibothropic and anti-inflammatory properties ofJ. elliptica.Methods:Phytochemical screening and thin-layer chromatography (TLC) assays were performed onJ. ellipticahydroalcoholic extract (TE) in order to observe its main constituents. The antibothropic activity of TE was evaluated by thein vitroneuromuscular blockade caused byBothrops jararacussuvenom (Bjssu), in a mouse phrenic nerve-diaphragm model (PND). A quantitative histological study was carried out to observe a possible protection of TE against the venom myotoxicity. The anti-inflammatory activity was also evaluated in two models, Bjssu-induced paw edema, and carrageenan-induced neutrophils migration in the peritoneal cavity.Results:TLC analysis revealed several compounds in TE, such as saponins, alkaloids, and phenolic constituents. TE was able to neutralize the blockade and the myotoxicity induced by venom, when it was pre-incubated for 30 min with venom. In addition, it showed anti-inflammatory activity, inducing less neutrophils migration and reducing paw edema.Conclusion:J. ellipticashowed both antibothropic and anti-inflammatory properties.
authors.affiliation: Environmental Sciences Post-Graduate Program, PGCiamb, Tocantins Federal University (UFT), Palmas, Tocantins, Brazil.
authors.forename: Sára Cósta
authors.initials: SC
authors.lastname: Ferreira-Rodrigues
authors.affiliation: Environmental Sciences Post-Graduate Program, PGCiamb, Tocantins Federal University (UFT), Palmas, Tocantins, Brazil.
authors.forename: Cássio Milhomens
authors.initials: CM
authors.lastname: Rodrigues
authors.affiliation: Environmental Sciences Post-Graduate Program, PGCiamb, Tocantins Federal University (UFT), Palmas, Tocantins, Brazil.
authors.forename: Marcio Galdino
authors.initials: MG
authors.lastname: Dos Santos
authors.affiliation: Post-Graduate Program in Pharmaceutical Sciences and Pharmacy, University of Sorocaba (UNISO), Sorocaba, SP, Brazil.
authors.forename: Jean Antonio Abraham
authors.initials: JA
authors.lastname: Gautuz
authors.affiliation: Post-Graduate Program in Pharmaceutical Sciences and Pharmacy, University of Sorocaba (UNISO), Sorocaba, SP, Brazil.
authors.forename: Magali Glauzer
authors.initials: MG
authors.lastname: Silva
authors.affiliation: Post-Graduate Program in Animal Production, University Camilo Castelo Branco (UNICASTELO), Descalvado, SP, Brazil.
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Cogo
authors.affiliation: Piracicaba Dental School, Department of Pharmacology, University of Campinas (UNICAMP), Piracicaba, SP Brazil.
authors.forename: Camila
authors.initials: C
authors.lastname: Batista-Silva
authors.affiliation: Piracicaba Dental School, Department of Pharmacology, University of Campinas (UNICAMP), Piracicaba, SP Brazil.
authors.forename: Cleiton Pita
authors.initials: CP
authors.lastname: Dos Santos
authors.affiliation: Piracicaba Dental School, Department of Pharmacology, University of Campinas (UNICAMP), Piracicaba, SP Brazil.
authors.forename: Francisco Carlos
authors.initials: FC
authors.lastname: Groppo
authors.affiliation: Piracicaba Dental School, Department of Pharmacology, University of Campinas (UNICAMP), Piracicaba, SP Brazil.; Faculty of Pharmaceutical Sciences, University of Campinas (UNICAMP), Rua Sérgio Buarque de Holanda, Campinas, SP, Brazil.
authors.forename: Karina
authors.initials: K
authors.lastname: Cogo-Müller
authors.affiliation: Post-Graduate Program in Pharmaceutical Sciences and Pharmacy, University of Sorocaba (UNISO), Sorocaba, SP, Brazil.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Oshima-Franco
keywords: Bothrops jararacussu; Edema; Inflammation; Jatropha elliptica; Myonecrosis; Neurotoxicity