title: Imbalance of Th17/Treg cells in pathogenesis of patients with human leukocyte antigen B27 associated acute anterior uveitis.
abstract: Th17 and regulatory T cells, involved in the pathogenesis of several autoimmune diseases, are new lineages of CD4+ T helper cells. However, the role of their imbalance in human leukocyte antigen B27-associated acute anterior uveitis has not been elucidated. In our study, the percentages of Th17 and Treg cells, their molecular markers and related factors in peripheral blood of patients and healthy controls were measured by flow cytometry, real-time RT-PCR and ELISA. We observed a remarkable increase of CD4+ and CD4+IL-17+ T cells in peripheral blood of patients compared to controls. The molecular markers and related factors of Th17 cell were also showed a distinct elevation. Interestingly, we observed an obvious decrease of CD4+CD25+Foxp3+ T cells and Foxp3 mRNA level in patients. The ratio of Th17/Treg in patients was dramatically higher than controls. Moreover, the ratio of Th17/Treg cells had a more significantly positive correlation with the disease activity score than Th17 cells whereas Treg cells had a negative correlation. Our findings demonstrated a distinct increase of Th17 cells and a significant decrease of Treg cells in patients compared to controls. The imbalance of Th17 and Treg cells may play a vital role in the pathogenesis of the disease.
authors.affiliation: Division of Clinical Lab, Eye Hospital of Wenzhou Medical University, Wenzhou 325035, Zhejiang, China.|School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou 325025, Zhejiang, China.
authors.forename: Zhenchao
authors.initials: Z
authors.lastname: Zhuang
authors.affiliation: Department of Ophthalmology, Eye Hospital of Wenzhou Medical University, Wenzhou 325035, Zhejiang, China.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Division of Clinical Lab, Eye Hospital of Wenzhou Medical University, Wenzhou 325035, Zhejiang, China.|School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou 325025, Zhejiang, China.
authors.forename: Gejing
authors.initials: G
authors.lastname: Zhu
authors.affiliation: Division of Clinical Lab, Eye Hospital of Wenzhou Medical University, Wenzhou 325035, Zhejiang, China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Division of Clinical Lab, Eye Hospital of Wenzhou Medical University, Wenzhou 325035, Zhejiang, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Mao
authors.affiliation: School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou 325025, Zhejiang, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Hong
authors.affiliation: Division of Clinical Lab, Eye Hospital of Wenzhou Medical University, Wenzhou 325035, Zhejiang, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Li
authors.affiliation: Division of Clinical Lab, Eye Hospital of Wenzhou Medical University, Wenzhou 325035, Zhejiang, China.|School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou 325025, Zhejiang, China.
authors.forename: Meiqin
authors.initials: M
authors.lastname: Zheng
mesh_terms: D000208:Acute Disease; D000328:Adult; D015704:CD4 Antigens / Q000378:metabolism; D016022:Case-Control Studies; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D005260:Female; D015796:HLA-B27 Antigen / Q000378:metabolism*; D006801:Humans; D016131:Lymphocyte Subsets / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D058504:Th17 Cells / Q000276:immunology*; D014157:Transcription Factors / Q000097:blood / Q000378:metabolism; D014606:Uveitis, Anterior / Q000097:blood / Q000276:immunology* / Q000473:pathology*
chemical_list: D015704:CD4 Antigens; D016207:Cytokines; D015796:HLA-B27 Antigen; D014157:Transcription Factors