title: One-Pot Enzymatic Total Synthesis of Presteffimycinone, an Early Intermediate of the Anthracycline Antibiotic Steffimycin Biosynthesis.
abstract: Early acting cyclases play critical roles in programming the polyketide biosynthesis toward certain, distinguished scaffolds. Starting from acetyl-CoA and malonyl-CoA, a one-pot enzymatic total synthesis of an anthracyclinone scaffold, presteffimycinone, was achieved by mixing polyketide synthase (PKS) and early post-PKS enzymes from the biosynthetic pathways of three different types of type II-PKS driven anticancer antibiotics, namely, the mithramycin (aureolic acid-type), gilvocarcin (rearranged angucycline-type), and steffimycin (anthracycline) pathways.
authors.affiliation: Harbor Branch Oceanographic Institute, Florida Atlantic University , 5600 US 1 North, Fort Pierce, Florida 34946, United States.
authors.forename: Guojun
authors.identifier: 0000-0001-6416-2744
authors.initials: G
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Haining
authors.initials: H
authors.lastname: Zhu
authors.forename: Jürgen
authors.initials: J
authors.lastname: Rohr
mesh_terms: D018943:Anthracyclines / Q000378:metabolism*; D000900:Anti-Bacterial Agents; D015394:Molecular Structure; D048630:Polyketide Synthases; D013302:Streptomyces
chemical_list: D018943:Anthracyclines; D000900:Anti-Bacterial Agents; C009944:steffimycin; D048630:Polyketide Synthases