title: Phagebiotics in treatment and prophylaxis of healthcare-associated infections.
abstract: We have developed a phagebiotic composition using 8 virulent bacteriophages (2 strains of each species) which are able to lyseAcinetobacter baumannii,Klebsiella pneumoniae,Pseudomonas aeruginosaandStaphylococcus aureus. The unique character of the developed composition is ensured by particular properties of each bacteriophage comprising the preparation, including their range of lytic activity toward specific bacterial pathogens, morphology of their plaques, cycle of their development, restriction profile of their DNAs, specificity of their genomes (based on complete genome sequencing), and other properties. The preparation did not produce any signs of acute or chronic intoxication in the experimental animals. Therapeutic and prophylactic efficiency of the phagebiotic composition was demonstrated in the prevention and treatment of the experimental acuteK. pneumoniaeinfection in mice. The investigations have shown that the preparation possesses a high therapeutic efficiency and is highly competitive with ciprofloxacin which is very effective against the infective strainK. pneumoniae. Our small-scale clinical trial was aimed to evaluate therapeutic effectiveness of the phagebiotic composition in an epidemiological emergency situation in an intensive care unit, caused by multi-resistant strains ofAcinetobacter baumannii,Klebsiella pneumoniaeandPseudomonas aeruginosa. Seventy nine per cent of the initial samples from 14 patients' endotracheal aspirate, blood and urine were contaminated. Twenty-four hours after the 3-day phage therapy (20 ml of cocktail at a titer for each phage 108pfu/ml were introduced intragastrically through a tube once a day) contamination level dropped to 21%. Hence the obtained results enabled us to create a new phagebiotic composition that may be used as an alternative to antibiotics to treat these healthcare-associated infections.
authors.affiliation: G. N. Gabrichevsky Research Institute for Epidemiology and Microbiology, Moscow, Russia; Bphage LLC, Moscow, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Aleshkin
authors.affiliation: N. N. Burdenko Research Institute for Neurosurgery , Moscow, Russia.
authors.forename: O N
authors.initials: ON
authors.lastname: Ershova
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology , Obolensk , Moscow region, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Volozhantsev
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology , Obolensk , Moscow region, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Svetoch
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology , Obolensk , Moscow region, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Popova
authors.affiliation: G. N. Gabrichevsky Research Institute for Epidemiology and Microbiology, Moscow, Russia; Bphage LLC, Moscow, Russia; Astrakhan State Medical University, Astrakhan, Russia.
authors.forename: E O
authors.initials: EO
authors.lastname: Rubalskii
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology , Obolensk , Moscow region, Russia.
authors.forename: A I
authors.initials: AI
authors.lastname: Borzilov
authors.affiliation: G. N. Gabrichevsky Research Institute for Epidemiology and Microbiology , Moscow, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Aleshkin
authors.affiliation: G. N. Gabrichevsky Research Institute for Epidemiology and Microbiology , Moscow, Russia.
authors.forename: S S
authors.initials: SS
authors.lastname: Afanas'ev
authors.affiliation: I. M. Sechenov First Moscow State Medical University , Moscow, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Karaulov
authors.affiliation: Astrakhan State Medical University , Astrakhan, Russia.
authors.forename: K M
authors.initials: KM
authors.lastname: Galimzyanov
authors.affiliation: Astrakhan State Medical University , Astrakhan, Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Rubalsky
authors.affiliation: G. N. Gabrichevsky Research Institute for Epidemiology and Microbiology , Moscow, Russia.
authors.forename: S S
authors.initials: SS
authors.lastname: Bochkareva
keywords: Acinetobacter baumannii; Klebsiella pneumoniae; MRSA; Pseudomonas aeruginosa; healthcare-associated infections; phagebiotics