title: Antinociceptive effect of Aristolochia trilobata stem essential oil and 6-methyl-5-hepten-2yl acetate, its main compound, in rodents.
abstract: Aristolochia trilobata L. is an aromatic plant, popularly known as "mil-homens", and its essential oil (EO) is generally used to treat colic, diarrhea and dysentery disorders. We evaluated the antinociceptive effect of A. trilobata stem EO and of its major compound, the (R)-(-)-6-methyl-5-hepten-2-yl acetate (sulcatyl acetate: SA), using acetic acid (0.85%)-induced writhing response and formalin-induced (20 μL of 1%) nociceptive behavior in mice. We also evaluated the EO and SA effect on motor coordination, using the rota-rod apparatus. EO (25, 50 and 100 mg/kg) or SA (25 and 50 mg/kg) reduced nociceptive behavior in the writhing test (p<0.001). EO (100 mg/kg) and SA (25 and 50 mg/kg) decreased the nociception on the first phase of the formalin test (p<0.05). On the second phase, EO (25: p<0.01; 50: p<0.05 and 100 mg/kg: p<0.001) and SA (25 and 50 mg/kg; p<0.001) reduced the nociceptive response induced by formalin. EO and SA were not able to cause changes in the motor coordination of animals. Together, our results suggest that EO has an analgesic profile and SA seems to be one of the active compounds in this effect.
authors.affiliation: Department of Physiology, Federal University of Sergipe, Sergipe, Brazil.
authors.forename: Jullyana de Souza Siqueira
authors.identifier: https://orcid.org/0000-0001-6507-8982
authors.initials: JSS
authors.lastname: Quintans
authors.affiliation: Department of Physiology, Federal University of Sergipe, Sergipe, Brazil.
authors.forename: Rafael Dos Santos
authors.initials: RDS
authors.lastname: Alves
authors.affiliation: Department of Chemistry, Federal University of Sergipe, Sergipe, Brazil.
authors.forename: Darlisson de Alexandria
authors.initials: DA
authors.lastname: Santos
authors.affiliation: Department of Pharmacy, Federal University of Sergipe, Sergipe, Brazil.
authors.forename: Mairim Russo
authors.initials: MR
authors.lastname: Serafini
authors.affiliation: Department of Chemistry, Federal University of Sergipe, Sergipe, Brazil.
authors.forename: Péricles Barreto
authors.initials: PB
authors.lastname: Alves
authors.affiliation: Department of Chemistry, Federal University of Amazonas, Amazonas, Brazil.
authors.forename: Emmanoel Vilaça
authors.initials: EV
authors.lastname: Costa
authors.affiliation: Science Faculty, Department of Biology, Selcuk University, Konya, Turkey.
authors.forename: Gokhan
authors.initials: G
authors.lastname: Zengin
authors.affiliation: Department of Physiology, Federal University of Sergipe, Sergipe, Brazil.
authors.forename: Lucindo José
authors.initials: LJ
authors.lastname: Quintans-Júnior
authors.affiliation: Department of Health Education, Federal University of Sergipe-UFS, Av. Governador Marcelo Déda, 13, Centro Lagarto/SE CEP 49400-000, Brazil, Tel.: +55 79 8867-5490, E-mail: adrianagibara@hotmail.com.
authors.forename: Adriana Gibara
authors.initials: AG
authors.lastname: Guimarães
mesh_terms: D000085:Acetates / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D030022:Aristolochia / Q000737:chemistry*; D019850:Heptanol / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification*; D010147:Pain Measurement; D010936:Plant Extracts / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry*; D011597:Psychomotor Performance / Q000187:drug effects; D045442:Rotarod Performance Test
chemical_list: D000085:Acetates; D000700:Analgesics; D009822:Oils, Volatile; D010936:Plant Extracts; D019850:Heptanol