title: Overexpression of microRNA-205 predicts lymph node metastasis and indicates an unfavorable prognosis in endometrial cancer.
abstract: As an integral component of the surgical staging system, lymphadenectomy for patients with endometrial cancer (EC) remains controversial, particularly in clinical stage I disease that includes not only low-risk, but also high-risk subgroups. In order to maximize the therapeutic effect of lymph node excision for high-risk patients who can potentially obtain survival benefits from it while minimizing its reverse effects in low-risk patients, pre-operative risk stratification of lymph node metastasis is necessary. The upregulation of microRNA-205 (miR-205) in carcinoma of the endometrium has been consistently reported recently and has been found to correlate with poor survival. The current study aimed to investigate whether the overexpression of miR-205 in curettage samples of EC could identify patients who are at a high risk for lymph node metastasis prior to surgery and validate the role of miR-205 as a prognostic marker in EC. Relative quantification detection of miR-205 in curettage and hysterectomy specimens of patients with EC was performed. Prediction of lymph node metastasis based on miR-205 expression, as well as tumor type and grade in curettage samples, was performed for all EC patients and patients with clinical stage I disease. Moreover, survival analysis was conducted. It was observed that miR-205 was significantly and consistently elevated in the curettage and hysterectomy samples of EC relative to normal controls. Furthermore, the overexpression of miR-205 could predict lymph node metastasis with a high accuracy and was revealed again to be associated with a poor prognosis in EC. Prospective and multicentric studies are required to further clarify the value of miR-205 as a promising predictor to stratify risk for lymph node metastasis in EC.
authors.affiliation: Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China; Department of Gynecology, General Hospital Affiliated to Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China; Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Yong-Jing
authors.initials: YJ
authors.lastname: Ma
authors.affiliation: Department of Gynecology, General Hospital Affiliated to Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China; Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Chun-Fang
authors.initials: CF
authors.lastname: Ha
authors.affiliation: Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Zhi-Miao
authors.initials: ZM
authors.lastname: Bai
authors.affiliation: Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Hai-Ning
authors.initials: HN
authors.lastname: Li
authors.affiliation: Department of Gynecology, General Hospital Affiliated to Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Jiang
keywords: endometrial cancer; lymph node metastasis; microRNA-205; prognosis