title: Morphology and Molecular Phylogeny ofRaillietinaspp. (Cestoda: Cyclophyllidea: Davaineidae) from Domestic Chickens in Thailand.
abstract: Raillietinaspecies are prevalent in domestic chickens (Gallus gallus domesticus) in Phayao province, northern Thailand. Their infection may cause disease and death, which affects the public health and economic situation in chicken farms. The identification ofRaillietinahas been based on morphology and molecular analysis. In this study, morphological observations using light (LM) and scanning electron microscopies (SEM) coupled with molecular analysis of the internal transcribed spacer 2 (ITS2) region and the nicotinamide adenine dinucleotide dehydrogenase subunit 1 (ND1) gene were employed for precise identification and phylogenetic relationship studies ofRaillietinaspp. FourRaillietinaspecies, includingR. echinobothrida, R. tetragona, R. cesticillus, andRaillietinasp., were recovered in domestic chickens from 4 districts in Phayao province, Thailand. LM and SEM observations revealed differences in the morphology of the scolex, position of the genital pore, number of eggs per egg capsule, and rostellar opening surface structures in all 4 species. Phylogenetic relationships were found among the phylogenetic trees obtained by the maximum likelihood and distance-based neighbor-joining methods. ITS2 and ND1 sequence data recorded fromRaillietinasp. appeared to be monophyletic. The query sequences ofR. echinobothrida, R. tetragona, R. cesticillus, andRaillietinasp. were separated according to the different morphological characters. This study confirmed that morphological studies combined with molecular analyses can differentiate related species within the genusRaillietinain Thailand.
authors.affiliation: Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Preeyaporn
authors.initials: P
authors.lastname: Butboonchoo
authors.affiliation: Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.|The Applied Technology for Biodiversity Research Unit, Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Chalobol
authors.initials: C
authors.lastname: Wongsawad
authors.affiliation: Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Amnat
authors.initials: A
authors.lastname: Rojanapaibul
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 03080, Korea.|Institute of Parasitic Diseases, Korea Association of Health Promotion, Seoul 07649, Korea.
authors.forename: Jong-Yil
authors.initials: JY
authors.lastname: Chai
mesh_terms: D000818:Animals; D002589:Cestoda / Q000033:anatomy & histology* / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002590:Cestode Infections / Q000469:parasitology / Q000662:veterinary*; D002645:Chickens; D016000:Cluster Analysis; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D008853:Microscopy; D009245:NADH Dehydrogenase / Q000235:genetics; D010802:Phylogeny; D011201:Poultry Diseases / Q000469:parasitology*; D017422:Sequence Analysis, DNA; D013785:Thailand
chemical_list: D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer; D009245:NADH Dehydrogenase
keywords: ITS2; ND1; Raillietina cesticillus; Raillietina echinobothrida; Raillietina sp.; Raillietina tetragona; Thailand; chicken; morphology; phylogeny