title: An Effective Bacterial Fucosidase for Glycoprotein Remodeling.
abstract: Fucose is an important component of many oligo- and polysaccharide structures as well as glycoproteins and glycolipids, which are often associated with a variety of physiological processes ranging from fertilization, embryogenesis, signal transduction, and disease progression, such as rheumatoid arthritis, inflammation, and cancer. The enzyme α-l-fucosidase is involved in the cleavage of the fucosidic bond in glycans and glycoconjugates, particularly the Fuc-α-1,2-Gal, Fuc-α-1,3/4-GlcNAc, and Fuc-α-1,6-GlcNAc linkages. Here, we report a highly efficient fucosidase, designated as BfFucH identified from a library of bacterial glycosidases expressed in E. coli from the CAZy database, which is capable of hydrolyzing the aforementioned fucosidic linkages, especially the α-1,6-linkage from the N-linked Fuc-α-1,6-GlcNAc residue on glycoproteins. Using BfFucH coupled with endoglycosidases and the emerging glycosynthases allows glycoengineering of IgG antibodies to provide homogeneous glycoforms with well-defined glycan structures and optimal effector functions.
authors.affiliation: Genomics Research Center, Academia Sinica , No. 128, Section 2, Academia Road, Taipei 115, Taiwan.|Institute of Biotechnology, National Taiwan University , Taipei 106, Taiwan.|Department of Chemistry, The Scripps Research Institute , 10550 North Torrey Pines Road, La Jolla, California 92037, United States.
authors.forename: Tsung-I
authors.identifier: 0000-0002-8355-6017
authors.initials: TI
authors.lastname: Tsai
authors.affiliation: Genomics Research Center, Academia Sinica , No. 128, Section 2, Academia Road, Taipei 115, Taiwan.
authors.forename: Shiou-Ting
authors.initials: ST
authors.lastname: Li
authors.affiliation: Genomics Research Center, Academia Sinica , No. 128, Section 2, Academia Road, Taipei 115, Taiwan.|Institute of Biotechnology, National Taiwan University , Taipei 106, Taiwan.
authors.forename: Chiu-Ping
authors.initials: CP
authors.lastname: Liu
authors.affiliation: Genomics Research Center, Academia Sinica , No. 128, Section 2, Academia Road, Taipei 115, Taiwan.|Department of Chemistry, University of Michigan , Ann Arbor, Michigan 48109, United States.
authors.forename: Karen Y
authors.initials: KY
authors.lastname: Chen
authors.affiliation: Genomics Research Center, Academia Sinica , No. 128, Section 2, Academia Road, Taipei 115, Taiwan.
authors.forename: Sachin S
authors.initials: SS
authors.lastname: Shivatare
authors.affiliation: Genomics Research Center, Academia Sinica , No. 128, Section 2, Academia Road, Taipei 115, Taiwan.
authors.forename: Chin-Wei
authors.initials: CW
authors.lastname: Lin
authors.affiliation: Genomics Research Center, Academia Sinica , No. 128, Section 2, Academia Road, Taipei 115, Taiwan.
authors.forename: Shih-Fen
authors.initials: SF
authors.lastname: Liao
authors.affiliation: Genomics Research Center, Academia Sinica , No. 128, Section 2, Academia Road, Taipei 115, Taiwan.
authors.forename: Chih-Wei
authors.initials: CW
authors.lastname: Lin
authors.affiliation: Genomics Research Center, Academia Sinica , No. 128, Section 2, Academia Road, Taipei 115, Taiwan.
authors.forename: Tsui-Ling
authors.initials: TL
authors.lastname: Hsu
authors.affiliation: Genomics Research Center, Academia Sinica , No. 128, Section 2, Academia Road, Taipei 115, Taiwan.
authors.forename: Ying-Ta
authors.initials: YT
authors.lastname: Wu
authors.affiliation: CHO Pharma Inc. , Taipei 11503, Taiwan.
authors.forename: Ming-Hung
authors.initials: MH
authors.lastname: Tsai
authors.affiliation: CHO Pharma Inc. , Taipei 11503, Taiwan.
authors.forename: Meng-Yu
authors.initials: MY
authors.lastname: Lai
authors.affiliation: CHO Pharma Inc. , Taipei 11503, Taiwan.
authors.forename: Nan-Horng
authors.initials: NH
authors.lastname: Lin
authors.affiliation: Genomics Research Center, Academia Sinica , No. 128, Section 2, Academia Road, Taipei 115, Taiwan.
authors.forename: Chung-Yi
authors.initials: CY
authors.lastname: Wu
authors.affiliation: Genomics Research Center, Academia Sinica , No. 128, Section 2, Academia Road, Taipei 115, Taiwan.|Institute of Biotechnology, National Taiwan University , Taipei 106, Taiwan.|Department of Chemistry, The Scripps Research Institute , 10550 North Torrey Pines Road, La Jolla, California 92037, United States.
authors.forename: Chi-Huey
authors.identifier: 0000-0002-9961-7865
authors.initials: CH
authors.lastname: Wong
mesh_terms: D001419:Bacteria / Q000201:enzymology* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism; D005643:Fucose / Q000737:chemistry / Q000378:metabolism*; D006001:Glycoconjugates / Q000737:chemistry / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity; D005644:alpha-L-Fucosidase / Q000378:metabolism*
chemical_list: D006001:Glycoconjugates; D006023:Glycoproteins; D007074:Immunoglobulin G; D011134:Polysaccharides; D005643:Fucose; D005644:alpha-L-Fucosidase