title: Self-assembling peptide nanostructures on aligned poly(lactide-co-glycolide) nanofibers for the functional regeneration of sciatic nerve.
abstract: OBJECTIVE
  Regeneration of functional peripheral nerve tissue at critical-sized defect requires extracellular matrix analogs impregnated with appropriate biosignals to regulate the cell fate process and subsequent tissue progression. The aim of the study was to develop electrospun aligned nanofibers as architectural analogs integrated with RADA16-I-BMHP1 as biofunctional peptides.
  
  
  METHODS
  Aligned poly(lactide-co-glycolide) (PLGA)-RADA16-I-BMHP1 nanofibers were fabricated and characterized for their in vitro potential using rat Schwann cell line and in vivo potential using a 10 mm sciatic nerve transection rat model.
  
  
  RESULTS
  PLGA-peptide scaffolds significantly promoted higher expression of genotypic markers and bipolar extension of Schwann cells. Further, PLGA-peptide treated animals promoted the native collagen organization, remyelination and showed significantly higher recovery of sensorimotor and motor function than PLGA-treated groups (p < 0.05).
  
  
  CONCLUSIONS
  Our results demonstrate that self-assembling peptide nanostructures on aligned PLGA nanofibers provided better cell-matrix communication with significant functional restoration of the sciatic nerve.
authors.affiliation: Centre for Nanotechnology & Advanced Biomaterials, School of Chemical & Biotechnology, SASTRA University, Thanjavur - 613401, Tamil Nadu, India.
authors.forename: Manasa
authors.initials: M
authors.lastname: Nune
authors.affiliation: Centre for Nanotechnology & Advanced Biomaterials, School of Chemical & Biotechnology, SASTRA University, Thanjavur - 613401, Tamil Nadu, India.
authors.forename: Anuradha
authors.initials: A
authors.lastname: Subramanian
authors.affiliation: Centre for Nanotechnology & Advanced Biomaterials, School of Chemical & Biotechnology, SASTRA University, Thanjavur - 613401, Tamil Nadu, India.
authors.forename: Uma Maheswari
authors.initials: UM
authors.lastname: Krishnan
authors.affiliation: Veterinary dispensary - Paliyode, Department of Animal Husbandry, Government of Kerala, Trivandrum - 695124, Kerala, India.
authors.forename: Suraj Sasidhara
authors.initials: SS
authors.lastname: Kaimal
authors.affiliation: Centre for Nanotechnology & Advanced Biomaterials, School of Chemical & Biotechnology, SASTRA University, Thanjavur - 613401, Tamil Nadu, India.
authors.forename: Swaminathan
authors.initials: S
authors.lastname: Sethuraman
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry; D005109:Extracellular Matrix; D006801:Humans; D008297:Male; D057139:Nanofibers / Q000737:chemistry*; D009416:Nerve Regeneration; D009842:Oligopeptides; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology; D011098:Polyglactin 910 / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects; D012584:Sciatic Nerve / Q000502:physiology*; D013499:Surface Properties
chemical_list: C000626774:BMHP1 peptide; D009842:Oligopeptides; D010455:Peptides; C546681:RADA16-I; D011098:Polyglactin 910; D003094:Collagen
keywords: RADA16; electrospinning; nanofibers; sciatic nerve; self-assembling peptides