title: Mouse Model of Hydroquinone Hypersensitivity via Innate and Acquired Immunity and its Promotion by Combined Reagents.
abstract: We established a mouse model of contact hypersensitivity (CHS) to hydroquinone (HQ), a widespread chemical in our environment. HQ was painted onto flanks; then, HQ was challenged by painting onto ear pinnas on days 7 and 14. The CHS after the second challenge was markedly greater than that after the first challenge. Both challenges increased thymic stromal lymphopoietin and T helper type 2 cytokines in ear pinnas, whereas IFN-γ (typical T helper type 1 cytokine) was decreased, despite an increase in IL-18 (typical IFN-γ inducer). In nude mice (T cell-reduced), although a first challenge induced CHS, a second challenge did not augment it. In severe combined immunodeficient, severe combined immunodeficient-beige, and IL-1-deficient mice, CHS was not induced. However, CHS was inducible in severe combined immunodeficient-beige mice after transfer of natural killer cells from HQ-sensitized normal mice. Tretinoin (used for enhancing the skin-whitening effect of HQ) and resin monomers (used to prevent polymerization of HQ) lowered the HQ concentration needed to establish sensitization to HQ. The augmented CHS after a second challenge was reduced by JNJ7777120, dexamethasone, suplatast tosilate (T helper type 2-cytokine inhibitor), and anti-thymic stromal lymphopoietin antibody. These results suggest that (i) thymic stromal lymphopoietin, IL-1, and T and/or natural killer cells are important in establishing and augmenting CHS to HQ and (ii) inflammatory chemicals may promote CHS to HQ as adjuvants.
authors.affiliation: Division of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate School of Dentistry, Sendai, Japan; Division of Molecular Regulation, Tohoku University Graduate School of Dentistry, Sendai, Japan. Electronic address: kananbando@dent.tohoku.ac.jp.
authors.forename: Kanan
authors.initials: K
authors.lastname: Bando
authors.affiliation: Division of Molecular Regulation, Tohoku University Graduate School of Dentistry, Sendai, Japan; Division of Pediatric Dentistry, Tohoku University Graduate School of Dentistry, Sendai, Japan.
authors.forename: Yukinori
authors.initials: Y
authors.lastname: Tanaka
authors.affiliation: Division of Molecular Regulation, Tohoku University Graduate School of Dentistry, Sendai, Japan.
authors.forename: Toshinobu
authors.initials: T
authors.lastname: Kuroishi
authors.affiliation: Division of Advanced Prosthetic Dentistry, Tohoku University Graduate School of Dentistry, Sendai, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Sasaki
authors.affiliation: Division of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate School of Dentistry, Sendai, Japan.
authors.forename: Teruko
authors.initials: T
authors.lastname: Takano-Yamamoto
authors.affiliation: Division of Molecular Regulation, Tohoku University Graduate School of Dentistry, Sendai, Japan.
authors.forename: Shunji
authors.initials: S
authors.lastname: Sugawara
authors.affiliation: Division of Molecular Regulation, Tohoku University Graduate School of Dentistry, Sendai, Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Endo
mesh_terms: D056704:Adaptive Immunity / Q000276:immunology*; D000818:Animals; D016207:Cytokines / Q000276:immunology; D003877:Dermatitis, Contact / Q000276:immunology*; D004195:Disease Models, Animal; D006873:Hydroquinones / Q000276:immunology*; D007113:Immunity, Innate / Q000276:immunology*; D007371:Interferon-gamma / Q000276:immunology; D007375:Interleukin-1 / Q000276:immunology; D020382:Interleukin-18 / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D013601:T-Lymphocytes / Q000276:immunology; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D016207:Cytokines; D006873:Hydroquinones; D007375:Interleukin-1; D020382:Interleukin-18; D007371:Interferon-gamma; C031927:hydroquinone; D000094632:Thymic Stromal Lymphopoietin; C000721967:TSLP protein, mouse