title: The genome sequence of Barbarea vulgaris facilitates the study of ecological biochemistry.
abstract: The genus Barbarea has emerged as a model for evolution and ecology of plant defense compounds, due to its unusual glucosinolate profile and production of saponins, unique to the Brassicaceae. One species, B. vulgaris, includes two 'types', G-type and P-type that differ in trichome density, and their glucosinolate and saponin profiles. A key difference is the stereochemistry of hydroxylation of their common phenethylglucosinolate backbone, leading to epimeric glucobarbarins. Here we report a draft genome sequence of the G-type, and re-sequencing of the P-type for comparison. This enables us to identify candidate genes underlying glucosinolate diversity, trichome density, and study the genetics of biochemical variation for glucosinolate and saponins. B. vulgaris is resistant to the diamondback moth, and may be exploited for "dead-end" trap cropping where glucosinolates stimulate oviposition and saponins deter larvae to the extent that they die. The B. vulgaris genome will promote the study of mechanisms in ecological biochemistry to benefit crop resistance breeding.
authors.affiliation: Department of Molecular Biology and Genetics, Aarhus University, Forsøgsvej 1, 4200 Slagelse, Denmark.|Crop Science Department, Teagasc, Oak Park, Ireland.
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Byrne
authors.affiliation: Department of Plant and Environmental Sciences and Copenhagen Plant Science Center, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark.
authors.forename: Pernille Østerbye
authors.initials: PØ
authors.lastname: Erthmann
authors.affiliation: Department of Plant and Environmental Sciences and Copenhagen Plant Science Center, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark.
authors.forename: Niels
authors.initials: N
authors.lastname: Agerbirk
authors.affiliation: Department of Plant and Environmental Sciences and Copenhagen Plant Science Center, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark.
authors.forename: Søren
authors.initials: S
authors.lastname: Bak
authors.affiliation: Department of Plant and Environmental Sciences and Copenhagen Plant Science Center, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark.
authors.forename: Thure Pavlo
authors.initials: TP
authors.lastname: Hauser
authors.affiliation: Department of Molecular Biology and Genetics, Aarhus University, Forsøgsvej 1, 4200 Slagelse, Denmark.
authors.forename: Istvan
authors.initials: I
authors.lastname: Nagy
authors.affiliation: Department of Molecular Biology and Genetics, Aarhus University, Forsøgsvej 1, 4200 Slagelse, Denmark.
authors.forename: Cristiana
authors.initials: C
authors.lastname: Paina
authors.affiliation: Department of Molecular Biology and Genetics, Aarhus University, Forsøgsvej 1, 4200 Slagelse, Denmark.
authors.forename: Torben
authors.initials: T
authors.lastname: Asp
mesh_terms: D031216:Barbarea / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D019295:Computational Biology / Q000379:methods; D060467:Disease Resistance / Q000235:genetics; D014644:Genetic Variation; D018745:Genome, Plant; D023281:Genomics / Q000379:methods; D005961:Glucosinolates / Q000378:metabolism; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D058977:Molecular Sequence Annotation; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D040641:Quantitative Trait Loci; D019655:Quantitative Trait, Heritable; D000073336:Whole Genome Sequencing
chemical_list: D005961:Glucosinolates; D010940:Plant Proteins