title: Trophic transfer and effects of gold nanoparticles (AuNPs) in Gammarus fossarum from contaminated periphytic biofilm.
abstract: This work addressed the trophic transfer and effects of functionalized gold nanoparticles (AuNPs) from periphytic biofilms to the crustacean Gammarus fossarum. Biofilms were exposed for 48 h to 10 nm positively charged functionalized AuNPs at two concentrations, 4.6 and 46 mg/L, and crustaceans G. fossarum grazed on these for 7 days, with daily biofilm renewal. Gold bioaccumulation in biofilm and crustacean were measured to estimate the trophic transfer ratio of these AuNP, and, for the first time, a transcriptomic approach and transmission electron microscopy observations in the crustacean were made. These two approaches showed cellular damage caused by oxidative stress and, in particular, an impact of these AuNPs on mitochondrial respiration. Modulation of digestive enzyme activity was also observed, suggesting modifications of digestive functions. The damage due to these nanoparticles could then have vital consequences for the organisms during chronic exposure.
authors.affiliation: University of Bordeaux, CNRS, UMR EPOC 5805, Aquatic Ecotoxicology, Place du Dr Peyneau, 33120, Arcachon, France. magalie.baudrimont@u-bordeaux.fr.
authors.forename: Magalie
authors.initials: M
authors.lastname: Baudrimont
authors.affiliation: University of Bordeaux, CNRS, UMR EPOC 5805, Aquatic Ecotoxicology, Place du Dr Peyneau, 33120, Arcachon, France.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Andrei
authors.affiliation: ICMCB, 87 Avenue du Dr Albert Schweitzer, 33600, Pessac, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Mornet
authors.affiliation: University of Bordeaux, CNRS, UMR EPOC 5805, Aquatic Ecotoxicology, Place du Dr Peyneau, 33120, Arcachon, France.
authors.forename: Patrice
authors.initials: P
authors.lastname: Gonzalez
authors.affiliation: University of Bordeaux, CNRS, UMR EPOC 5805, Aquatic Ecotoxicology, Place du Dr Peyneau, 33120, Arcachon, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Mesmer-Dudons
authors.affiliation: University of Bordeaux, CNRS, UMR EPOC 5805, Aquatic Ecotoxicology, Place du Dr Peyneau, 33120, Arcachon, France.
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Gourves
authors.affiliation: Université de Reims Champagne Ardenne, UMR-I 02 SEBIO, Campus du Moulin de la Housse, BP 1039, 51687, Reims cedex 2, France.
authors.forename: Ali
authors.initials: A
authors.lastname: Jaffal
authors.affiliation: Université de Reims Champagne Ardenne, UMR-I 02 SEBIO, Campus du Moulin de la Housse, BP 1039, 51687, Reims cedex 2, France.
authors.forename: Odile
authors.initials: O
authors.lastname: Dedourge-Geffard
authors.affiliation: Université de Reims Champagne Ardenne, UMR-I 02 SEBIO, Campus du Moulin de la Housse, BP 1039, 51687, Reims cedex 2, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Geffard
authors.affiliation: IRSTEA, 5 rue de la Doua, CS70077, 69626, Villeurbanne Cedex, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Geffard
authors.affiliation: IRSTEA, 5 rue de la Doua, CS70077, 69626, Villeurbanne Cedex, France.
authors.forename: Jeanne
authors.initials: J
authors.lastname: Garric
authors.affiliation: University of Bordeaux, CNRS, UMR EPOC 5805, Aquatic Ecotoxicology, Place du Dr Peyneau, 33120, Arcachon, France.
authors.forename: Agnès
authors.initials: A
authors.lastname: Feurtet-Mazel
mesh_terms: D033304:Amphipoda / Q000187:drug effects*; D000818:Animals; D018441:Biofilms / Q000187:drug effects*; D006046:Gold / Q000737:chemistry / Q000494:pharmacology*; D053768:Metal Nanoparticles / Q000737:chemistry; D046529:Microscopy, Electron, Transmission
chemical_list: D006046:Gold
keywords: Digestive enzyme activity; Gammarus fossarum; Gene transcription; Gold nanoparticles; Mitochondrial disruption; Periphytic biofilm; Trophic transfer