title: Electrochemical sensing platform based on molecularly imprinted polymer decorated N,S co-doped activated graphene for ultrasensitive and selective determination of cyclophosphamide.
abstract: A novel electrochemical sensor has been facilely fabricated by applying composite elements consisting of nitrogen and sulfur co-doped activated graphene (N,S-AGR) and molecularly imprinted polymer (MIP). N,S-AGR was synthesized by one-pot pyrogenation of a mixture of thiourea, KOH and graphene oxide, which was introduced to improve electron transfer capability and surface area of the electrode, while the electro-polymerized MIP layer afforded simultaneous recognition and quantification of cyclophosphamide (CPA) by utilizing Fe(CN)63-/4-as probe to indicate electrical signals. Under the optimal conditions, a calibration curve of current shift versus concentration of CPA was got in the range of 8×10-12-8×10-7molL-1, and the developed sensor gave a remarkably low detection limit (LOD) of 3.4×10-12mol L-1(S/N=3). Moreover, the as-prepared sensor illustrated other good merits like stability and selectivity, and played a role in real-time therapeutic drug monitoring after CPA administration in rabbit.
authors.affiliation: Key Laboratory of Xinjiang Phytomedicine Resources for Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832000, China.
authors.forename: Bintong
authors.initials: B
authors.lastname: Huang
authors.affiliation: Laboratory of Environmental Science and Technology, The Xinjiang Technical Institute of Physics and Chemistry; Key Laboratory of Functional Materials and Devices for Special Environments, Chinese Academy of Sciences, Urumqi 830011, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing 100083, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Dong
authors.affiliation: Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing 100083, China.
authors.forename: Xueji
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Science, Harbin Institute of Technology, Shenzhen 518055, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gan
authors.affiliation: Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
authors.forename: Shahid
authors.initials: S
authors.lastname: Mahboob
authors.affiliation: Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
authors.forename: Khalid Abdullah
authors.initials: KA
authors.lastname: Al-Ghanim
authors.affiliation: School of Materials Science and Engineering, Harbin Institute of Technology, Shenzhen 518055, China. Electronic address: yuanqunhui@hitsz.edu.cn.
authors.forename: Qunhui
authors.initials: Q
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Xinjiang Phytomedicine Resources for Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832000, China; Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, China; School of Science, Harbin Institute of Technology, Shenzhen 518055, China. Electronic address: yingchunli@shzu.edu.cn.
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D002138:Calibration; D003520:Cyclophosphamide / Q000032:analysis*; D016903:Drug Monitoring; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D008958:Models, Molecular; D008968:Molecular Conformation; D054802:Molecular Imprinting; D009584:Nitrogen / Q000737:chemistry; D010084:Oxidation-Reduction; D011108:Polymers / Q000138:chemical synthesis*; D013455:Sulfur / Q000737:chemistry
chemical_list: D011108:Polymers; D013455:Sulfur; D006108:Graphite; D003520:Cyclophosphamide; D009584:Nitrogen
keywords: Cyclophosphamide; Drug monitoring; Electrochemical sensor; Nitrogen and sulfur co-doped activated grapheme