title: Arcobacter lekithochrous sp. nov., isolated from a molluscan hatchery.
abstract: Four bacterial strains, LFT 1.7T, LT2C 2.5, LT4C 2.8 and TM 4.6, were isolated from great scallop (Pecten maximus) larvae and tank seawater in a Norwegian hatchery and characterized by a polyphasic approach including determination of phenotypic, chemotaxonomic and genomic traits. All were Gram-stain-negative, motile rods, oxidase- and catalase-positive and required sea salts for growth. Major fatty acids present were summed feature 3 (C16 : 1ω7c/C16 : 1ω6c), summed feature 8 (C18 : 1ω7c or C18 : 1ω6c), C16 : 0, C14 : 0, summed feature 2 (C14 : 0 3-OH/iso-C16 : 1 I), C12 : 0 3-OH and C12 : 0. Strain LFT 1.7T contained menaquinone MK-6 as the sole respiratory quinone. Phylogenetic analysis based on 16S rRNA gene sequences indicated that all strains formed a distinct lineage within the genus Arcobacter with a low similarity to known species (94.77-95.32 %). The DNA G+C content was 28.7 mol%. Results of in silico DNA-DNA hybridization and average nucleotide identity confirmed that the isolates constitute a novel species of Arcobacter, for which the name Arcobacter lekithochrous sp. nov. is proposed. The type strain is LFT 1.7T (=CECT 8942T=DSM 100870T).
authors.affiliation: Departamento de Microbiología y Parasitología, CIBUS-Facultad de Biología, Universidade de Santiago, 15782 Santiago de Compostela, Spain.
authors.forename: Ana L
authors.initials: AL
authors.lastname: Diéguez
authors.affiliation: Departamento de Microbiología y Parasitología, CIBUS-Facultad de Biología, Universidade de Santiago, 15782 Santiago de Compostela, Spain.
authors.forename: Sabela
authors.initials: S
authors.lastname: Balboa
authors.affiliation: Department of Biology, Faculty of Mathematics and Natural Sciences, University of Bergen, Bergen, Norway.
authors.forename: Thorolf
authors.initials: T
authors.lastname: Magnesen
authors.affiliation: Departamento de Microbiología y Parasitología, CIBUS-Facultad de Biología, Universidade de Santiago, 15782 Santiago de Compostela, Spain.
authors.forename: Jesús L
authors.initials: JL
authors.lastname: Romalde
mesh_terms: D000818:Animals; D017756:Aquaculture; D020640:Arcobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D009664:Norway; D009693:Nucleic Acid Hybridization; D049910:Pecten / Q000382:microbiology*; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6