title: Characterization of mitochondrial DNA polymorphisms in the Han population in Liaoning Province, Northeast China.
abstract: This study characterized the genetic variations of mitochondrial DNA (mtDNA) to elucidate the maternal genetic structure of Liaoning Han Chinese. A total of 317 blood samples of unrelated individuals were collected for analysis in Liaoning Province. The mtDNA samples were analyzed using two distinct methods: sequencing of the hypervariable sequences I and II (HVSI and HVSII), and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the coding region. The results indicated a high gene diversity value (0.9997 ± 0.0003), a high polymorphism information content (0.99668) and a random match probability (0.00332). These samples were classified into 305 haplotypes, with 9 shared haplotypes. The most common haplogroup was D4 (12.93%). The principal component analysis map, the phylogenetic tree map, and the genetic distance matrix all indicated that the genetic distance of the Liaoning Han population from the Tibetan group was distant, whereas that from the Miao group was relatively close.
authors.affiliation: a School of Forensic Medicine , China Medical University , Shenyang , China.
authors.forename: Feng-Ling
authors.initials: FL
authors.lastname: Xu
authors.affiliation: a School of Forensic Medicine , China Medical University , Shenyang , China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yao
authors.affiliation: a School of Forensic Medicine , China Medical University , Shenyang , China.
authors.forename: Mei
authors.initials: M
authors.lastname: Ding
authors.affiliation: a School of Forensic Medicine , China Medical University , Shenyang , China.
authors.forename: Zhang-Sen
authors.initials: ZS
authors.lastname: Shi
authors.affiliation: a School of Forensic Medicine , China Medical University , Shenyang , China.
authors.forename: Xue
authors.initials: X
authors.lastname: Wu
authors.affiliation: a School of Forensic Medicine , China Medical University , Shenyang , China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Zhang
authors.affiliation: a School of Forensic Medicine , China Medical University , Shenyang , China.
authors.forename: Bao-Jie
authors.initials: BJ
authors.lastname: Wang
mesh_terms: D044466:Asian People / Q000208:ethnology* / Q000235:genetics*; D002681:China / Q000208:ethnology; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D008297:Male; D000072741:Maternal Inheritance; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D025341:Principal Component Analysis
chemical_list: D004272:DNA, Mitochondrial
keywords: Liaoning Han; Mitochondrial DNA; forensic medicine; haplogroup; polymorphism