title: Glycerol-3-Phosphate Acyltransferase 3 (OsGPAT3) is required for anther development and male fertility in rice.
abstract: Lipid molecules are key structural components of plant male reproductive organs, such as the anther and pollen. Although advances have been made in the understanding of acyl lipids in plant reproduction, the metabolic pathways of other lipid compounds, particularly glycerolipids, are not fully understood. Here we report that an endoplasmic reticulum-localized enzyme, Glycerol-3-Phosphate Acyltransferase 3 (OsGPAT3), plays an indispensable role in anther development and pollen formation in rice. OsGPAT3 is preferentially expressed in the tapetum and microspores of the anther. Compared with wild-type plants, the osgpat3 mutant displays smaller, pale yellow anthers with defective anther cuticle, degenerated pollen with defective exine, and abnormal tapetum development and degeneration. Anthers of the osgpat3 mutant have dramatic reductions of all aliphatic lipid contents. The defective cuticle and pollen phenotype coincide well with the down-regulation of sets of genes involved in lipid metabolism and regulation of anther development. Taking these findings together, this work reveals the indispensable role of a monocot-specific glycerol-3-phosphate acyltransferase in male reproduction in rice.
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Men
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Wanqi
authors.initials: W
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Qianfei
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Gaibin
authors.initials: G
authors.lastname: Lian
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Quan
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Zhijing
authors.initials: Z
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Mingjiao
authors.initials: M
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.|School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia, Australia.
authors.forename: Dabing
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D015536:Down-Regulation; D005298:Fertility; D035264:Flowers / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012275:Oryza / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D005992:Glycerol-3-Phosphate O-Acyltransferase
keywords: Anther development; glycerol-3-phosphate acyltransferase; lipid metabolism; male sterility; microgametophyte; rice; tapetum