title: Localization of Toll-like Receptor (TLR) 2 and TLR4 mRNA in the Colorectal Mucosa of Miniature Dachshunds with Inflammatory Colorectal Polyps.
abstract: Inflammatory colorectal polyps (ICRPs) are characterized by the formation of multiple or solitary polyps with marked neutrophil infiltration in the colorectal area, and are speculated to be a novel form of breed-specific canine idiopathic inflammatory bowel disease (IBD). In human IBD, toll-like receptor (TLR) 2 and TLR4 have been reported to be involved in the pathogenesis of the disease. The aim of this study was to evaluate the expression of TLR2 and TLR4 mRNA in the colorectal mucosa of dogs with ICRPs by in-situ hybridization using an RNAscope assay. Samples of inflamed colorectal mucosa (n = 5) and non-inflamed mucosa (n = 5) from miniature dachshunds (MDs) with ICRPs and colonic mucosa from healthy beagles (n = 5) were examined. TLR2 and TLR4 hybridization signals were localized to the colorectal epithelium, inflammatory cells and fibroblasts in the inflamed colorectal mucosa of affected dogs. The signals were significantly greater in inflamed colorectal epithelium compared with non-inflamed epithelium of MDs with ICRPs and healthy beagles (P <0.05). These results suggest that increased expression of TLR2 and TLR4 mRNA in the inflamed colorectal mucosa results from not only inflammatory cell infiltration, but also the upregulation of TLR2 and TLR4 mRNA in the colonic epithelium.
authors.affiliation: Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Sapporo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Yokoyama
authors.affiliation: Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Sapporo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ohta
authors.affiliation: Laboratory of Molecular Medicine, Department of Veterinary Clinical Sciences, Sapporo, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Yamazaki
authors.affiliation: Department of Diagnostic Pathology, Sapporo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kagawa
authors.affiliation: Laboratory of Anatomy, Department of Biomedical Science, Sapporo, Japan.
authors.forename: O
authors.initials: O
authors.lastname: Ichii
authors.affiliation: Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Sapporo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Khoirun
authors.affiliation: Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Sapporo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Morita
authors.affiliation: Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Sapporo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Osuga
authors.affiliation: Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Sapporo, Japan.
authors.forename: S Y
authors.initials: SY
authors.lastname: Lim
authors.affiliation: Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Sapporo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Sasaki
authors.affiliation: Veterinary Teaching Hospital, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Morishita
authors.affiliation: Veterinary Teaching Hospital, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.affiliation: Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Sapporo, Japan. Electronic address: mtaki@vetmed.hokudai.ac.jp.
authors.forename: M
authors.initials: M
authors.lastname: Takiguchi
mesh_terms: D000818:Animals; D003111:Colonic Polyps / Q000662:veterinary*; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D007091:Image Processing, Computer-Assisted; D017403:In Situ Hybridization; D015212:Inflammatory Bowel Diseases / Q000662:veterinary*; D007413:Intestinal Mucosa / Q000378:metabolism; D012333:RNA, Messenger; D012007:Rectum; D051195:Toll-Like Receptor 2 / Q000032:analysis / Q000096:biosynthesis*; D051197:Toll-Like Receptor 4 / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D012333:RNA, Messenger; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4
keywords: inflammatory colorectal polyp; miniature dachshund; pattern recognition receptor; toll-like receptor