title: The association between HERG gene expression and cardiac arrhythmia disease in children.
abstract: We explored the possible link between the expression ofHERGgene and cardiomyopathy in children. From April 2013 to April 2015, 73 children with cardiac arrhythmia who were treated were enrolled in the present study to serve as the observation group. At the same time, 76 normal individuals were also enrolled as the control group. HERG expression level in the observation group was compared with the control group. To determine the level ofHERGgene expression we used fluorescent directional PCR, enzyme immunoassay and western blot analysis. The results showed that HERG mRNA level in the observation group was significantly higher than that of the control group. The level of HERG protein in the observation group was significantly higher as well. In the observation group, HERG expression gradually increased with time during the course of the disease. This result suggested thatHERGgene expression was associated with the severity of cardiac arrhythmia in children. HERG expression may be the cause of deterioration in cardiomyopathy. The results have provided a theoretical and practical basis for the diagnosis and treatment of children cardiomyopathy. Thus, we established a correlation between HERG expression and cardiac arrhythmia in children.
authors.affiliation: Department of Cardiology, Xuzhou Children's Hospital, Xuzhou, Jiangsu 221002, P.R. China.
authors.forename: Mingyu
authors.initials: M
authors.lastname: Fu
authors.affiliation: Department of Cardiology, Xuzhou Children's Hospital, Xuzhou, Jiangsu 221002, P.R. China.
authors.forename: Chunli
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Cardiology, Xuzhou Children's Hospital, Xuzhou, Jiangsu 221002, P.R. China.
authors.forename: Qingwen
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Xuzhou Children's Hospital, Xuzhou, Jiangsu 221002, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Xuzhou Children's Hospital, Xuzhou, Jiangsu 221002, P.R. China.
authors.forename: Xinjiang
authors.initials: X
authors.lastname: An
keywords: HERG gene; RT-PCR; cardiac arrhythmia of children; correlation; enzyme-linked immunosorbent assay