title: CASPASE-8 gene polymorphisms (rs13416436 and rs2037815) are not associated with preeclampsia development in Brazilian women.
abstract: BACKGROUND
  Preeclampsia is responsible for considerable mortality and morbidity of mother and sibling. The etiology of preeclampsia is still unknown. Family studies indicate the involvement of genes located on chromosome 2 in preeclampsia development. Considering the importance of apoptosis and chromosome 2, one promising candidate for the study of the genetic cause of this syndrome is the CASPASE-8 gene, which was chosen as the subject of this study.
  
  
  OBJECTIVE
  The aim of this study was to determine the frequencies of the genotypes for CASP8 gene polymorphisms (rs13416436 and rs2037815) and to associate these with preeclampsia development in Brazilian women.
  
  
  METHODS
  Women with and without preeclampsia were investigated. Accordingly, peripheral blood was collected and DNA extracted, followed by genotyping using Real-time PCR with hydrolysis probe (Taqman®Life Technologies).
  
  
  RESULTS
  The results showed no association between genotypes and preeclampsia development for both polymorphisms studied (χ2=1.03; p = 0.59, for rs13416436 and χ2=1.06; p = 0.58 for rs2037815).
  
  
  CONCLUSIONS
  It seems that CASP8 gene polymorphisms (rs13416436 and rs2037815) are not important candidates for the development of preeclampsia. Other genes related to the apoptosis process or other polymorphisms in this gene should be studied in order to understand better the etiology of preeclampsia.
authors.affiliation: a Curso de Graduação em Medicina , Universidade Federal do Triângulo Mineiro , Uberaba , Brazil.
authors.forename: Ivanir Celso
authors.initials: IC
authors.lastname: Orlando
authors.affiliation: b Programa de Pós-Graduação em Ciências da Saúde , Universidade Federal do Triângulo Mineiro , Uberaba , Brazil.
authors.forename: Sarah Cristina Sato Vaz
authors.identifier: http://orcid.org/0000-0003-4466-6093
authors.initials: SCSV
authors.lastname: Tanaka
authors.affiliation: c Disciplina de Genética, Instituto de Ciências Biológicas e Naturais , Universidade Federal do Triângulo Mineiro , Uberaba , Brazil.
authors.forename: Marly Aparecida Spadotto
authors.initials: MAS
authors.lastname: Balarin
authors.affiliation: d Programa de Pós-Graduação em Atenção à Saúde , Universidade Federal do Triângulo Mineiro , Uberaba , Brazil.
authors.forename: Sueli Riul
authors.initials: SR
authors.lastname: da Silva
authors.affiliation: d Programa de Pós-Graduação em Atenção à Saúde , Universidade Federal do Triângulo Mineiro , Uberaba , Brazil.|e Departamento de Pediatria e Genética, Centro de Ciências Médicas , Universidade Federal da Paraíba , João Pessoa , Brazil.
authors.forename: Cristina Wide
authors.identifier: http://orcid.org/0000-0002-5534-8544
authors.initials: CW
authors.lastname: Pissetti
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D053181:Caspase 8 / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D055815:Young Adult
chemical_list: C505530:CASP8 protein, human; D053181:Caspase 8
keywords: Apoptosis; caspase-8; genetic; genetic predisposition to disease; hypertension; polymorphism; pregnancy-induced