title: Depletion of NFBD1/MDC1 Induces Apoptosis in Nasopharyngeal Carcinoma Cells Through the p53-ROS-Mitochondrial Pathway.
abstract: NFBD1, a signal amplifier of the p53 pathway, is vital for protecting cells from p53-mediated apoptosis and the early phase of DNA damage response under normal culture conditions. Here we investigated its expression in patients with nasopharyngeal carcinoma (NPC), and we describe the biological functions of the NFBD1 gene. We found that NFBD1 mRNA and protein were more highly expressed in NPC tissues than in nontumorous tissues. To investigate the function of NFBD1, we created NFBD1-depleted NPC cell lines that exhibited decreased cellular proliferation and colony formation, an increase in their rate of apoptosis, and an enhanced sensitivity to chemotherapeutic agents compared with in vitro controls. However, N-acetyl cysteine (NAC) and downregulation of p53 expression could partially reverse the apoptosis caused by the loss of NFBD1. Further analysis showed that loss of NFBD1 resulted in increased production of intracellular reactive oxygen species (ROS) depending on p53, which subsequently triggered the mitochondrial apoptotic pathway. Using a xenograft model in nude mice, we showed that silencing NFBD1 also significantly inhibited tumor growth and led to apoptosis. Taken together, our data suggest that inhibition of NFBD1 in NPC could be therapeutically useful.
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical UniversityChongqingP.R. China.
authors.forename: Zhihai
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Chongqing Medical UniversityChongqingP.R. China.
authors.forename: Kui
authors.initials: K
authors.lastname: Liao
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical UniversityChongqingP.R. China.
authors.forename: Wenqi
authors.initials: W
authors.lastname: Zuo
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical UniversityChongqingP.R. China.
authors.forename: Xueliang
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical UniversityChongqingP.R. China.
authors.forename: Zhili
authors.initials: Z
authors.lastname: Qiu
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical UniversityChongqingP.R. China.
authors.forename: Zhitao
authors.initials: Z
authors.lastname: Gong
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical UniversityChongqingP.R. China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical UniversityChongqingP.R. China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Zeng
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical UniversityChongqingP.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Qian
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical UniversityChongqingP.R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Biochemistry and Molecular Biology, Molecular Medicine and Cancer Research, China Center, Chongqing Medical UniversityChongqingP.R. China.
authors.forename: Youquan
authors.initials: Y
authors.lastname: Bu
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical UniversityChongqingP.R. China.
authors.forename: Suling
authors.initials: S
authors.lastname: Hong
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical UniversityChongqingP.R. China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Hu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000328:Adult; D000368:Aged; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002277:Carcinoma; D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D020868:Gene Silencing; D064593:Heterografts; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018797:Cell Cycle Proteins; C413163:MDC1 protein, human; D009687:Nuclear Proteins; D017382:Reactive Oxygen Species; D015534:Trans-Activators; D016159:Tumor Suppressor Protein p53