title: Discovery of 1,2,3-Triazole Derivatives for Multimodality PET/CT/Cryoimaging of Myelination in the Central Nervous System.
abstract: Myelin pathology is present in many neurological conditions such as multiple sclerosis (MS) and traumatic spinal cord injury (SCI). To facilitate development of novel therapies aimed at myelin repair, we set out to develop imaging agents that permit direct quantification of myelination in vivo. In this work, we designed and synthesized a series of fluorescent fluorinated myelin imaging agents that can be used for in vivo positron emission tomography (PET) imaging combined with subsequent post-mortem fluorescent cryoimaging. Structure-activity relationship (SAR) studies of the newly developed myelin imaging agents led us to identify a lead compound (TAFDAS, 21) that readily enters the brain and spinal cord and selectively binds to myelin. By conducting sequential PET and 3D cryoimaging in an SCI rat model, we demonstrated for the first time that PET and cryoimaging can be combined as a novel technique to image the spinal cord with high sensitivity and spatial resolution.
authors.forename: Chunying
authors.identifier: 0000-0002-6782-2610
authors.initials: C
authors.lastname: Wu
authors.forename: Brendan
authors.initials: B
authors.lastname: Eck
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhang
authors.forename: Junqing
authors.initials: J
authors.lastname: Zhu
authors.forename: Anand Dev
authors.initials: AD
authors.lastname: Tiwari
authors.forename: Yifan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yunjie
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Nuclear Medicine, PLA General Hospital , Beijing 100853, China.
authors.forename: Jinming
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Radiology, Bingzhou Medical University , Binzhou, Shandong 256603, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Radiology, Bingzhou Medical University , Binzhou, Shandong 256603, China.
authors.forename: Xizhen
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Radiology, Bingzhou Medical University , Binzhou, Shandong 256603, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.forename: Jingqiang
authors.initials: J
authors.lastname: You
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Yihui
authors.initials: Y
authors.lastname: Guan
authors.forename: Xingdang
authors.initials: X
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Neurosciences, Cleveland Clinic , Cleveland, Ohio 44195, United States.
authors.forename: Bruce D
authors.initials: BD
authors.lastname: Trapp
authors.affiliation: Department of Anatomy and Regenerative Biology, George Washington University , Washington, D.C. 20037, United States.
authors.forename: Robert
authors.initials: R
authors.lastname: Miller
authors.forename: Jerry
authors.initials: J
authors.lastname: Silver
authors.forename: David
authors.initials: D
authors.lastname: Wilson
authors.affiliation: Department of Radiology, Bingzhou Medical University , Binzhou, Shandong 256603, China.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000000981:diagnostic imaging*; D005453:Fluorescence; D051379:Mice; D064847:Multimodal Imaging; D009186:Myelin Sheath / Q000378:metabolism*; D049268:Positron-Emission Tomography; D014057:Tomography, X-Ray Computed; D014230:Triazoles / Q000008:administration & dosage*
chemical_list: D014230:Triazoles