title: Candidate SNP markers of aggressiveness-related complications and comorbidities of genetic diseases are predicted by a significant change in the affinity of TATA-binding protein for human gene promoters.
abstract: Aggressiveness in humans is a hereditary behavioral trait that mobilizes all systems of the body-first of all, the nervous and endocrine systems, and then the respiratory, vascular, muscular, and others-e.g., for the defense of oneself, children, family, shelter, territory, and other possessions as well as personal interests. The level of aggressiveness of a person determines many other characteristics of quality of life and lifespan, acting as a stress factor. Aggressive behavior depends on many parameters such as age, gender, diseases and treatment, diet, and environmental conditions. Among them, genetic factors are believed to be the main parameters that are well-studied at the factual level, but in actuality, genome-wide studies of aggressive behavior appeared relatively recently. One of the biggest projects of the modern science-1000 Genomes-involves identification of single nucleotide polymorphisms (SNPs), i.e., differences of individual genomes from the reference genome. SNPs can be associated with hereditary diseases, their complications, comorbidities, and responses to stress or a drug. Clinical comparisons between cohorts of patients and healthy volunteers (as a control) allow for identifying SNPs whose allele frequencies significantly separate them from one another as markers of the above conditions. Computer-based preliminary analysis of millions of SNPs detected by the 1000 Genomes project can accelerate clinical search for SNP markers due to preliminary whole-genome search for the most meaningful candidate SNP markers and discarding of neutral and poorly substantiated SNPs.
  
  
  
  Here, we combine two computer-based search methods for SNPs (that alter gene expression) {i} Web service SNP_TATA_Comparator (DNA sequence analysis) and {ii} PubMed-based manual search for articles on aggressiveness using heuristic keywords. Near the known binding sites for TATA-binding protein (TBP) in human gene promoters, we found aggressiveness-related candidate SNP markers, including rs1143627 (associated with higher aggressiveness in patients undergoing cytokine immunotherapy), rs544850971 (higher aggressiveness in old women taking lipid-lowering medication), and rs10895068 (childhood aggressiveness-related obesity in adolescence with cardiovascular complications in adulthood).
  
  
  
  After validation of these candidate markers by clinical protocols, these SNPs may become useful for physicians (may help to improve treatment of patients) and for the general population (a lifestyle choice preventing aggressiveness-related complications).
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10 Lavrentyev Avenue, Novosibirsk, 630090, Russia.|Novosibirsk State University, 2 Pirogova Street, Novosibirsk, 630090, Russia.
authors.forename: Irina V
authors.initials: IV
authors.lastname: Chadaeva
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10 Lavrentyev Avenue, Novosibirsk, 630090, Russia. pon@bionet.nsc.ru.|Novosibirsk State University, 2 Pirogova Street, Novosibirsk, 630090, Russia. pon@bionet.nsc.ru.
authors.forename: Mikhail P
authors.initials: MP
authors.lastname: Ponomarenko
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10 Lavrentyev Avenue, Novosibirsk, 630090, Russia.
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Rasskazov
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10 Lavrentyev Avenue, Novosibirsk, 630090, Russia.
authors.forename: Ekaterina B
authors.initials: EB
authors.lastname: Sharypova
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10 Lavrentyev Avenue, Novosibirsk, 630090, Russia.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Kashina
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10 Lavrentyev Avenue, Novosibirsk, 630090, Russia.
authors.forename: Marina Yu
authors.initials: MY
authors.lastname: Matveeva
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10 Lavrentyev Avenue, Novosibirsk, 630090, Russia.
authors.forename: Tatjana V
authors.initials: TV
authors.lastname: Arshinova
authors.affiliation: Children's Hospital Los Angeles, 4640 Hollywood Boulevard, University of Southern California, Los Angeles, CA, 90027, USA.
authors.forename: Petr M
authors.initials: PM
authors.lastname: Ponomarenko
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10 Lavrentyev Avenue, Novosibirsk, 630090, Russia.|Vector-Best Inc, Koltsovo, Novosibirsk Region, 630559, Russia.
authors.forename: Olga V
authors.initials: OV
authors.lastname: Arkova
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10 Lavrentyev Avenue, Novosibirsk, 630090, Russia.
authors.forename: Natalia P
authors.initials: NP
authors.lastname: Bondar
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10 Lavrentyev Avenue, Novosibirsk, 630090, Russia.
authors.forename: Ludmila K
authors.initials: LK
authors.lastname: Savinkova
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10 Lavrentyev Avenue, Novosibirsk, 630090, Russia.|Novosibirsk State University, 2 Pirogova Street, Novosibirsk, 630090, Russia.
authors.forename: Nikolay A
authors.initials: NA
authors.lastname: Kolchanov
mesh_terms: D000483:Alleles; D018450:Disease Progression; D005260:Female; D056726:Genetic Association Studies; D030342:Genetic Diseases, Inborn / Q000150:complications / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D009765:Obesity / Q000150:complications / Q000235:genetics; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D035181:TATA-Box Binding Protein / Q000378:metabolism*; D016896:Treatment Outcome
chemical_list: D005819:Genetic Markers; D035181:TATA-Box Binding Protein
keywords: Aggressiveness; Candidate SNP marker; Gene; Keyword-based search; Prediction in silico; Promoter; Single nucleotide polymorphism; TATA-binding protein