title: Silencing of Armadillo Repeat-Containing Protein 8 (ARMc8) Inhibits TGF-β-Induced EMT in Bladder Carcinoma UMUC3 Cells.
abstract: Armadillo repeat-containing protein 8 (ARMc8) is a key factor in regulating cell migration, proliferation, tissue maintenance, and tumorigenesis. However, its role in bladder cancer remains unknown. Thus, in this study we sought to investigate the effect of ARMc8 on the epithelial-to-mesenchymal transition (EMT) progress in bladder cancer cells induced by transforming growth factor-β1 (TGF-β1). Our results found that ARMc8 was highly expressed in bladder cancer cell lines. ARMc8 silencing inhibited the TGF-β1-induced migration and invasion and suppressed the EMT progress in bladder cancer cells. Furthermore, ARMc8 silencing inhibited the TGF-β1-induced expression of β-catenin, cyclin D1, and c-myc in bladder cancer cells. In conclusion, the present study demonstrates a novel function for ARMc8, which acts as a mediator for TGF-β1-induced cell migration/invasion through modulation of the Wnt/β-catenin signaling pathway in bladder cancer cells. This study suggests that ARMc8 may be a potential therapeutic target for the development of therapies for bladder cancer.
authors.affiliation: Department of Oncology, The First Affiliated Hospital, Xian Jiaotong University Medical CollegeXian, ShaanxiP.R. China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Liang
authors.affiliation: Department of Urology, The Central Hospital of BaojiBaoji, ShaanxiP.R. China.
authors.forename: Qun-Li
authors.initials: QL
authors.lastname: Men
authors.affiliation: Department of Urology, The Central Hospital of WeinanWeinan, ShaanxiP.R. China.
authors.forename: Yong-Wei
authors.initials: YW
authors.lastname: Li
authors.affiliation: Department of Urology, The Second Affiliated Hospital, Xian Jiaotong University Medical CollegeXian, ShaanxiP.R. China.
authors.forename: He-Cheng
authors.initials: HC
authors.lastname: Li
authors.affiliation: Department of Urology, The Second Affiliated Hospital, Xian Jiaotong University Medical CollegeXian, ShaanxiP.R. China.
authors.forename: Tie
authors.initials: T
authors.lastname: Chong
authors.affiliation: Department of Urology, The Second Affiliated Hospital, Xian Jiaotong University Medical CollegeXian, ShaanxiP.R. China.
authors.forename: Zhao-Lun
authors.initials: ZL
authors.lastname: Li
mesh_terms: D051186:Armadillo Domain Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects / Q000235:genetics*; D015870:Gene Expression; D020868:Gene Silencing; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000378:metabolism / Q000494:pharmacology; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000378:metabolism*; D060449:Wnt Signaling Pathway / Q000187:drug effects
chemical_list: C522393:ARMC8 protein, human; D051186:Armadillo Domain Proteins; D034741:RNA, Small Interfering; D016212:Transforming Growth Factor beta