title: Iron Availability Compromises Not Only Oligodendrocytes But Also Astrocytes and Microglial Cells.
abstract: When disrupted, iron homeostasis negatively impacts oligodendrocyte (OLG) differentiation and impairs myelination. To better understand myelin formation and OLG maturation, in vivo and in vitro studies were conducted to evaluate the effect of iron deficiency (ID) not only on OLG maturation but also on astrocytes (AST) and microglial cells (MG). In vivo experiments in an ID model were carried out to describe maturational events during OLG and AST development and the reactive profile of MG during myelination when iron availability is lower than normal. In turn, in vitro assays were conducted to explore proliferating and maturational states of each glial cell type derived from control or ID conditions. Studies targeted NG2, PDGFRα, CNPAse, CC1, and MBP expression in OLG, GFAP and S100 expression in AST, and CD11b, ED1, and cytokine expression in MG, as well as BrDU incorporation in the three cell types. Our results show that ID affected OLG development at early stages, not only reducing their maturation capacity but also increasing their proliferation and affecting their morphological complexity. AST ID proliferated more than control ones and were more immature, much like OLG. Cytokine expression in ID animals reflected an anti-inflammatory state which probably influenced OLG maturation. These results show that ID conditions alter all glial cells and may impact myelin formation, which could be regulated by a mechanism involving a cross talk between AST, MG, and oligodendrocyte progenitors (OPC).
authors.affiliation: Departamento de Química Biológica, Facultad de Farmacia y Bioquímica. IQUIFIB-CONICET, Universidad de Buenos Aires, Junín 956, C1113, Buenos Aires, Argentina.
authors.forename: Maria Victoria
authors.initials: MV
authors.lastname: Rosato-Siri
authors.affiliation: Departamento de Química Biológica, Facultad de Farmacia y Bioquímica. IQUIFIB-CONICET, Universidad de Buenos Aires, Junín 956, C1113, Buenos Aires, Argentina.
authors.forename: Leandro
authors.initials: L
authors.lastname: Marziali
authors.affiliation: Departamento de Química Biológica, Facultad de Farmacia y Bioquímica. IQUIFIB-CONICET, Universidad de Buenos Aires, Junín 956, C1113, Buenos Aires, Argentina.
authors.forename: María Eugenia
authors.initials: ME
authors.lastname: Guitart
authors.affiliation: Departamento de Química Biológica, Facultad de Farmacia y Bioquímica. IQUIFIB-CONICET, Universidad de Buenos Aires, Junín 956, C1113, Buenos Aires, Argentina.
authors.forename: Maria Elvira
authors.initials: ME
authors.lastname: Badaracco
authors.affiliation: Instituto Leloir - IIBBA-CONICET, Buenos Aires, Argentina.
authors.forename: Mariana
authors.initials: M
authors.lastname: Puntel
authors.affiliation: Instituto Leloir - IIBBA-CONICET, Buenos Aires, Argentina.
authors.forename: Fernando
authors.initials: F
authors.lastname: Pitossi
authors.affiliation: Instituto de Investigaciones Neurológicas Dr. Raúl Carrea, FLENI, Buenos Aires, Argentina.
authors.forename: Jorge
authors.initials: J
authors.lastname: Correale
authors.affiliation: Departamento de Química Biológica, Facultad de Farmacia y Bioquímica. IQUIFIB-CONICET, Universidad de Buenos Aires, Junín 956, C1113, Buenos Aires, Argentina. jpasquin@qb.ffyb.uba.ar.
authors.forename: Juana Maria
authors.initials: JM
authors.lastname: Pasquini
mesh_terms: D018798:Anemia, Iron-Deficiency / Q000378:metabolism*; D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D001921:Brain / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D006706:Homeostasis / Q000502:physiology; D007501:Iron / Q000378:metabolism*; D017628:Microglia / Q000378:metabolism*; D009186:Myelin Sheath / Q000378:metabolism; D009836:Oligodendroglia / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007501:Iron
keywords: Astrocyte response; Hypomyelination; Iron deficiency; Microglial cell activation; Oligodendrocyte maturation