title: Immuno-PCR technology for detection of natural human antibodies against Lecdisaccharide.
abstract: The development of an immuno-PCR assay for quantitation of low amounts of anti-glycan human antibodies is described. The sensitivity of the assay for determination of low-affinity anti-LeCIgM has been found to be 4 ng/ml (~100 pg per sample), thus being two orders of magnitude higher compared to the conventional ELISA with the same antigen.
authors.affiliation: JSC NPF DNA-Technology, Moscow, Russia. maerle@yandex.ru.
authors.forename: Artem V
authors.initials: AV
authors.lastname: Maerle
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Daria V
authors.initials: DV
authors.lastname: Voronina
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Kira L
authors.initials: KL
authors.lastname: Dobrochaeva
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Oxana E
authors.initials: OE
authors.lastname: Galanina
authors.affiliation: NRC Institute of Immunology, Federal Biomedical Agency of Russia, Moscow, Russia.
authors.forename: Leonid P
authors.initials: LP
authors.lastname: Alekseev
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Nicolai V
authors.initials: NV
authors.lastname: Bovin
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Sergei K
authors.initials: SK
authors.lastname: Zavriev
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Dmitriy Yu
authors.initials: DY
authors.lastname: Ryazantsev
mesh_terms: D004187:Disaccharides / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis* / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004187:Disaccharides; D007075:Immunoglobulin M
keywords: Glycans; Immuno-PCR; LeC; Natural antibodies