title: Overexpression of ARF1 is associated with cell proliferation and migration through PI3K signal pathway in ovarian cancer.
abstract: ADP-ribosylation factor 1 (ARF1) is a small G protein that regulates many cellular processes such as reorganization of the actin cytoskeleton and is highly expressed in various tumor cells and tissues. However, the role of ARF1 in ovarian cancer progression remains unknown. In the present study, we explored the expression patterns of ARF1 in clinical ovarian cancer samples and adjacent noncancerous tissues. The results revealed that ARF1 overexpressed in EOC tissues and cell lines, compared with the adjacent non-tumorous tissues and normal ovarian cells. In addition, the immunoreactivity of ARF1 was positively correlated with EOC grade and Ki-67 expression. Knockdown of ARF1 expression notably inhibited cell proliferation and migration rate of EOC cells by the auxiliary of PI3K. Taken together, our findings provide new insights into the functional role of ARF1 on EOC cell growth and migration and it may serve as a diagnostic and therapeutic target.
authors.affiliation: Department of Obstetrics and Gynecology, Nantong Third People's Hospital, Nantong University, Nantong, Jiangsu 226001, P.R. China.
authors.forename: Guifang
authors.initials: G
authors.lastname: Gu
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong University, Nantong, Jiangsu 226001, P.R. China.
authors.forename: Yannan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Science and Education, The Second People's Hospital of Nantong, Nantong, Jiangsu 226002, P.R. China.
authors.forename: Chengwei
authors.initials: C
authors.lastname: Duan
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Maternal and Child Care Service Centre, Nantong University, Nantong, Jiangsu 226001, P.R. China.
authors.forename: Linsen
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Obstetrics and Gynecology, Yixing People's Hospital, Wuxi, Jiangsu 214200, P.R. China.
authors.forename: Caiwen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Oncology, Jiangyin People's Hospital, Jiangyin, Jiangsu 214401, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong University, Nantong, Jiangsu 226001, P.R. China.
authors.forename: Jialin
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong University, Nantong, Jiangsu 226001, P.R. China.
authors.forename: Nannan
authors.initials: N
authors.lastname: Shi
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong University, Nantong, Jiangsu 226001, P.R. China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong University, Nantong, Jiangsu 226001, P.R. China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Xi
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong University, Nantong, Jiangsu 226001, P.R. China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Zhong
mesh_terms: D020823:ADP-Ribosylation Factor 1 / Q000378:metabolism*; D018262:Adenocarcinoma, Clear Cell / Q000378:metabolism / Q000556:secondary*; D002288:Adenocarcinoma, Mucinous / Q000378:metabolism / Q000556:secondary*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D014408:Biomarkers, Tumor; D015153:Blotting, Western; D002453:Cell Cycle; D049109:Cell Proliferation; D018284:Cystadenocarcinoma, Serous / Q000378:metabolism / Q000556:secondary*; D016889:Endometrial Neoplasms / Q000378:metabolism / Q000556:secondary*; D005260:Female; D005455:Fluorescent Antibody Technique; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D047468:Immunoprecipitation; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D011379:Prognosis; D014407:Tumor Cells, Cultured; D014945:Wound Healing; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D019869:Phosphatidylinositol 3-Kinases; D020823:ADP-Ribosylation Factor 1