title: Molecular and Functional Characterization of Three Odorant-Binding Protein from Periplaneta americana.
abstract: The American cockroach, Periplaneta americana, is a vector of many pathogenic organisms associated with human diseases. Olfaction plays a crucial role in guiding cockroach behaviors and contributes to their ability to transmit pathogens. Odorant binding proteins (OBPs), abundant in the insect olfactory sensilla, are important for insect olfaction. In this study, three OBP genes, PameOBP1, 2 and 3, were cloned from P. americana. Sequence alignment and phylogenetic analysis revealed that PameOBP1, 2 and 3 belong to the Minus-C OBP, Classic OBP, and Plus-C OBP subfamilies, respectively. Expression pattern and ligand-binding analysis showed that PameOBP1 and 2 were specifically expressed in antennae, and exhibited high binding affinities (Ki < 2 μM) to farnesene, farnesol, 2-tridecanone, and tetradecane, suggesting roles in volatile perception. Conversely, PameOBP3 was ubiquitously expressed in most of the tissues examined at high levels and displayed very weak binding affinities (Ki > 40 μM) for all 87 ligands tested. Our study provides insights into the functional diversity of PameOBP genes and provides some volatiles that can potentially be used in behavioral interference of P. americana.
authors.affiliation: Key Laboratory of Tea Biology and Resource Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou, China.
authors.forename: Zhao-Qun
authors.initials: ZQ
authors.lastname: Li
authors.affiliation: State Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: He
authors.affiliation: College of Life Sciences, Huaibei Normal University, Huaibei, China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Zhang
authors.affiliation: College of Plant Protection, Nanjing Agricultural University/Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing, China.
authors.forename: Shuang-Lin
authors.initials: SL
authors.lastname: Dong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D057506:Arthropod Antennae / Q000378:metabolism / Q000473:pathology; D005260:Female; D019476:Insect Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D007659:Ketones / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D008297:Male; D010526:Periplaneta / Q000378:metabolism*; D010802:Phylogeny; D011485:Protein Binding; D012313:RNA / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D018035:Receptors, Odorant / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D016415:Sequence Alignment; D013050:Spectrometry, Fluorescence
chemical_list: D019476:Insect Proteins; D007659:Ketones; D008024:Ligands; D018035:Receptors, Odorant; D011994:Recombinant Proteins; C045648:odorant-binding protein; C009541:2-tridecanone; D012313:RNA