title: Characterization of 16 microsatellite markers for theOreinotinusclade ofViburnum(Adoxaceae).
abstract: OBJECTIVE
  Microsatellite loci were isolated from four species ofViburnum(Adoxaceae) to study population structure and assess species boundaries among morphologically similar South AmericanViburnumspecies of theOreinotinusclade.
  
  
  RESULTS
  Using a microsatellite-enriched library and mining next-generation sequence data, 16 microsatellites were developed. Each locus was tested on two populations ofV. triphyllumand one population ofV. pichinchense. For nuclear loci, one to 13 alleles were recovered, expected heterozygosity ranged from 0 to 0.8975, Simpson diversity index ranged from 0.0167 to 1.000, and Shannon diversity index ranged from 0 to 2.3670 in a given population. For the mitochondrial locus, three to six alleles were recovered and unbiased haploid diversity values ranged from 0.756 to 0.853 in a given population.
  
  
  CONCLUSIONS
  The 16 microsatellite loci developed for theOreinotinusclade (Viburnum, Adoxaceae) will inform investigations of population structure and species boundaries within this group.
authors.affiliation: Department of Biology, The College of New Jersey, 2000 Pennington Road, Ewing, New Jersey 08628 USA.
authors.forename: Syndi
authors.initials: S
authors.lastname: Barish
authors.affiliation: Facultad de Ciencias Biológicas and Museo de Historia Natural, Universidad Nacional Mayor de San Marcos, Av. Arenales 1256, Apdo. 14-0434, Lima 14, Peru.
authors.forename: Mónica
authors.initials: M
authors.lastname: Arakaki
authors.affiliation: Department of Ecology and Evolutionary Biology, Brown University, Box G-W, Providence, Rhode Island 02912 USA.
authors.forename: Erika J
authors.initials: EJ
authors.lastname: Edwards
authors.affiliation: Department of Ecology and Evolutionary Biology, Yale University, P.O. Box 208106, New Haven, Connecticut 06520 USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Donoghue
authors.affiliation: Department of Biology, The College of New Jersey, 2000 Pennington Road, Ewing, New Jersey 08628 USA.
authors.forename: Wendy L
authors.initials: WL
authors.lastname: Clement
keywords: Adoxaceae; Viburnum dentatum; Viburnum hallii; Viburnum pichinchense; Viburnum trilobum; Viburnum triphyllum; genetic diversity