title: PlasmamiR-21expression: an indicator for the severity of Type 2 diabetes with diabetic retinopathy.
abstract: To investigate the roles of plasmamiR-21in the pathogenic process of Type 2 diabetes (T2D) with diabetic retinopathy (DR). T2D patients included patients without DR (NDR) group, patients with non-proliferative/background DR (BDR) group and patients with proliferative DR (PDR) group. Healthy individuals served as control group. Fasting plasma glucose (FPG), glycosylated haemoglobin (HbA1c), triacylglycerol (TG), total cholesterol (TC), urine creatinine (Cr), fasting blood glucose (FBG), blood urea nitrogen (BUN), low-density lipoprotein cholesterol (LDL-C), fasting insulin (FINS) and plasmamiR-21expression were measured. Quantitative real-time PCR (qRT-PCR) was applied to detectmiR-21expression. Pearson analysis was used to conduct correlation analysis and receiver operating characteristic (ROC) curve was used to analyse the diagnostic value ofmiR-21in T2D with DR. Compared with the control group, FBG and HbA1c increased in the NDR group; compared with the control and NDR groups, disease course, HbA1c, FPG levels and homoeostasis model assessment of insulin resistance (HOMA-IR) were increased in the BDR and PDR groups; and compared with the BDR group, disease course, HbA1c and FPG levels were higher in the PDR group.miR-21expression was higher in the BDR group than the control group, and higher in the PDR group than the BDR group.miR-21expression was positively related with disease course, HbA1C, FPG and HOMA-IR, and had diagnostic value for T2D with DR and PDR. The plasmamiR-21expression was increased in the development of T2D with DR and can be used as an indicator for the severity of T2D with DR.
authors.affiliation: Department of Nephrology, China-Japan Union Hospital of Jilin University, Changchun 130033, P.R. China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: Department of Ophthalmology, China-Japan Union Hospital of Jilin University, Changchun 130033, P.R. China.
authors.forename: Xue-Man
authors.initials: XM
authors.lastname: Lyu
authors.affiliation: Department of Ophthalmology, China-Japan Union Hospital of Jilin University, Changchun 130033, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Department of Ophthalmology, China-Japan Union Hospital of Jilin University, Changchun 130033, P.R. China lingww_ww@163.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D015992:Body Mass Index; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000150:complications; D003930:Diabetic Retinopathy / Q000097:blood* / Q000209:etiology; D005260:Female; D005951:Glucose Tolerance Test; D006801:Humans; D007333:Insulin Resistance; D016014:Linear Models; D008297:Male; D055442:Metabolome / Q000502:physiology; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D012720:Severity of Illness Index
chemical_list: D015415:Biomarkers; C524940:MIRN21 microRNA, human; D035683:MicroRNAs
keywords: Background diabetic retinopathy; Diabetic retinopathy; Linear regression; Plasma microRNA-21; Proliferative diabetic retinopathy; Type 2 diabetes