title: Binding induced strand displacement amplification for homogeneous protein assay.
abstract: An ultrasensitive and homogenous strategy for protein assay was established based upon binding-induced strand displacement amplification (BI-SDA). Binding-Induced DNA strand-displacement occurred between Apt-T•signal DNA and Apt-C, and release of signal DNA upon addition of platelet-derived growth factor (PDGF BB). The released signal DNA further hybridized with multifunctional hairpin DNA probe and induced the strand-displacement amplification in the presence of Klenow Fragment (exo-) and dNTPs. The BI-SDA product contain G-quaruplex DNA, which could be recognized and reported by the fluorescence of fluorochrome N-methyl porphyrin propionic acid IX (NMM). The fluorescence intensity was proportional to the concentration of PDGF-BB over the range of 1.0×10-11mol/L -2.0×10-9mol/L, with a detection limit of 3.6pmol/L. This proposed strategy showed good selectivity and practicality, and might be applied to other proteins in the future.
authors.affiliation: College of Science, Guangdong Ocean University, Zhanjiang 524088, PR China. Electronic address: 2007liyubin@163.com.
authors.forename: Yubin
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Science, Guangdong Ocean University, Zhanjiang 524088, PR China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Science, Guangdong Ocean University, Zhanjiang 524088, PR China.
authors.forename: Zike
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: College of Science, Guangdong Ocean University, Zhanjiang 524088, PR China.
authors.forename: Yuner
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: College of Science, Guangdong Ocean University, Zhanjiang 524088, PR China.
authors.forename: Zirui
authors.initials: Z
authors.lastname: Wang
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D000077214:Becaplermin; D015374:Biosensing Techniques / Q000379:methods*; D015342:DNA Probes / Q000235:genetics / Q000378:metabolism; D020574:Proto-Oncogene Proteins c-sis / Q000032:analysis* / Q000378:metabolism
chemical_list: D052157:Aptamers, Nucleotide; D015342:DNA Probes; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin
keywords: Binding-induced strand displacement amplification; Homogeneous assay; Platelet-derived growth factor; Protein