title: Dimethyl-2-[(acridin-9-yl)methylidene]-malonate as fluorescent probe for histochemical analysis.
abstract: Fluorescent compounds have been widely used for biomolecule labeling in cytochemistry and histochemistry analysis. Here, it is described the optical properties of dimethyl 2-[(acridin-9-yl)methylidene]-malonate (LPSF/IP-81), an acridine derivative. This compound was conjugated to Concanavalin A (Con A) lectin and applied as sugar probe in lectin histochemistry. Evaluation of luminescent properties showed that LPSF/IP-81 is photoluminescent with excitation at 360 nm and emission at 428 nm. Con A hemagglutinating activity and LPSF/IP81 photoluminescence were unaltered after conjugation. Circular dichroism of Con A-LPSF/IP81 conjugate showed the maintenance of the Con A structure. Lectin histochemistry with Con A-LPSF/IP81 conjugate demonstrated different pattern recognition studying normal, fibroadenoma, and invasive ductal carcinoma of human breast. These findings indicate that LPSF/IP-81 can be proposed as an alternative probe for histochemical analysis.
authors.affiliation: Laboratório de Imunopatologia Keizo Asami (LIKA), Departamento de Bioquímica, Universidade Federal de Pernambuco (UFPE), Recife, PE, 50670-901, Brazil.|Faculdade de Ciências, Educação e Tecnologia de Garanhuns (FACETEG), Universidade de Pernambuco (UPE), Garanhuns, PE, 55290-000, Brazil.
authors.forename: Sinara Mônica Vitalino
authors.identifier: http://orcid.org/0000-0002-2605-4192
authors.initials: SMV
authors.lastname: Almeida
authors.affiliation: Laboratório de Imunopatologia Keizo Asami (LIKA), Departamento de Bioquímica, Universidade Federal de Pernambuco (UFPE), Recife, PE, 50670-901, Brazil.
authors.forename: Lúcia Patrícia Bezerra Gomes
authors.initials: LPBG
authors.lastname: Silva
authors.affiliation: Laboratório de Imunopatologia Keizo Asami (LIKA), Departamento de Bioquímica, Universidade Federal de Pernambuco (UFPE), Recife, PE, 50670-901, Brazil.
authors.forename: Luiza Rayanna Amorim
authors.initials: LRA
authors.lastname: Lima
authors.affiliation: Laboratório de Química e Inovação Terapêutica (LQIT), Departamento de Antibióticos, Universidade Federal de Pernambuco (UFPE), Recife, PE, 50670-901, Brazil.
authors.forename: Sandra Paula Sarinho
authors.initials: SPS
authors.lastname: Botelho
authors.affiliation: Laboratório de Química e Inovação Terapêutica (LQIT), Departamento de Antibióticos, Universidade Federal de Pernambuco (UFPE), Recife, PE, 50670-901, Brazil.
authors.forename: Maria do Carmo
authors.initials: MDC
authors.lastname: Lima
authors.affiliation: Núcleo de Pesquisas em Inovação Terapêutica (Nupit), Universidade Federal de Pernambuco (UFPE), Recife, PE, 50670-901, Brazil.
authors.forename: Ivan da Rocha
authors.initials: IDR
authors.lastname: Pitta
authors.affiliation: Laboratório de Imunopatologia Keizo Asami (LIKA), Departamento de Bioquímica, Universidade Federal de Pernambuco (UFPE), Recife, PE, 50670-901, Brazil.
authors.forename: Eduardo Isidoro Carneiro
authors.initials: EIC
authors.lastname: Beltrão
authors.affiliation: Laboratório de Imunopatologia Keizo Asami (LIKA), Departamento de Bioquímica, Universidade Federal de Pernambuco (UFPE), Recife, PE, 50670-901, Brazil.
authors.forename: Luiz Bezerra
authors.initials: LB
authors.lastname: Carvalho Júnior
mesh_terms: D000166:Acridines / Q000737:chemistry*; D001940:Breast / Q000000981:diagnostic imaging; D001943:Breast Neoplasms / Q000000981:diagnostic imaging*; D018270:Carcinoma, Ductal, Breast / Q000000981:diagnostic imaging*; D002942:Circular Dichroism; D003208:Concanavalin A / Q000737:chemistry*; D005260:Female; D018226:Fibroadenoma / Q000000981:diagnostic imaging*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D037102:Lectins / Q000032:analysis*; D008314:Malonates / Q000737:chemistry*
chemical_list: D000166:Acridines; D005456:Fluorescent Dyes; D037102:Lectins; D008314:Malonates; C000628362:dimethyl-2-((acridin-9-yl)methylidene)-malonate; D003208:Concanavalin A
keywords: Concanavalin A; acridine derivative; histochemical probe