title: Protective effect of Fragaria ananassa methanolic extract on cadmium chloride (CdCl2)-induced hepatotoxicity in rats.
abstract: This study investigated the protective effect of Fragaria ananassa methanolic extract on cadmium chloride (CdCl2)-induced hepatotoxicity in rats. CdCl2was intraperitoneally injected at a dose of 6.5 mg/kg of body weight for 5 d with or without methanol extract of Fragaria ananassa (250 mg/kg). The hepatic cadmium concentration, lipid peroxidation, nitric oxide, glutathione (GSH) content, and antioxidant enzyme activities, including superoxide dismutase, catalase (CAT), GSH peroxidase, and GSH reductase, were estimated. CdCl2injection induced a significant elevation in cadmium concentration, lipid peroxidation, and nitric oxide and caused a significant depletion in GSH content compared to controls, along with a remarkable decrease in antioxidant enzymes. Oxidative stress induction and cadmium accumulation in the liver were successfully ameliorated by F. ananassa (strawberry) pre-administration. In addition, the pre-administration of strawberry decreased the elevated gene expression of the pro-apoptotic Bax gene as well as the protein expression of caspases-3 in the liver of CdCl2-injected rats. In addition, the reduced gene expression of anti-apoptotic Bcl-2 was increased. Our results show an increase in the expression of tumor necrosis factor α in the liver of rats treated with cadmium. In sum, our results suggested that F. ananassa successfully prevented deleterious effects on liver function by reinforcing the antioxidant defense system, inhibiting oxidative stress and reducing apoptosis.
authors.affiliation: a Department of Zoology , College of Science, King Saud University , Riyadh , Saudi Arabia.|b Department of Zoology and Entomology, Faculty of Science , Helwan University , Cairo , Egypt.
authors.forename: Manal F
authors.initials: MF
authors.lastname: Elkhadragy
authors.affiliation: b Department of Zoology and Entomology, Faculty of Science , Helwan University , Cairo , Egypt.
authors.forename: Ahmed E
authors.initials: AE
authors.lastname: Abdel Moneim
mesh_terms: D000818:Animals; D019256:Cadmium Chloride / Q000493:pharmacokinetics / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D004195:Disease Models, Animal; D031985:Fragaria / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D007150:Immunohistochemistry; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D008111:Liver Function Tests; D008297:Male; D000432:Methanol / Q000737:chemistry; D010936:Plant Extracts / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D020011:Protective Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D017208:Rats, Wistar
chemical_list: D010936:Plant Extracts; D020011:Protective Agents; D019256:Cadmium Chloride; D000432:Methanol
keywords: Fragaria ananassa; apoptosis; cadmium chloride; liver; oxidative stress