title: Effects of photoperiod regimes and ultraviolet-C radiations on biosynthesis of industrially important lignans and neolignans in cell cultures of Linum usitatissimum L. (Flax).
abstract: Lignans and neolignans are principal bioactive components of Linum usitatissimum L. (Flax), having multiple pharmacological activities. In present study, we are reporting an authoritative abiotic elicitation strategy of photoperiod regimes along with UV-C radiations. Cell cultures were grown in different photoperiod regimes (24h-dark, 24h-light and 16L/8D h photoperiod) either alone or in combination with various doses (1.8-10.8kJ/m2) of ultraviolet-C (UV-C) radiations. Secoisolariciresinol diglucoside (SDG), lariciresinol diglucoside (LDG), dehydrodiconiferyl alcohol glucoside (DCG), and guaiacylglycerol-β-coniferyl alcohol ether glucoside (GGCG) were quantified by using reverse phase-high performance liquid chromatography (RP-HPLC). Results showed that the cultures exposed to UV-C radiations, accumulated higher levels of lignans, neolignans and other biochemical markers than cultures grown under different photoperiod regimes. 3.6kJ/m2dose of UV-C radiations resulted in 1.86-fold (7.1mg/g DW) increase in accumulation of SDG, 2.25-fold (21.6mg/g DW) in LDG, and 1.33-fold (9.2mg/g DW) in GGCG in cell cultures grown under UV+photoperiod than their respective controls. Furthermore, cell cultures grown under UV+dark showed 1.36-fold (60.0mg/g DW) increase in accumulation of DCG in response to 1.8kJ/m2dose of UV-C radiations. Smilar trends were observed in productivity of SDG, LDG and GGCG. Additionally, 3.6kJ/m2dose of UV-C radiations also resulted in 2.82-fold (195.65mg/l) increase in total phenolic production, 2.94-fold (98.9mg/l) in total flavonoid production and 1.04-fold (95%) in antioxidant activity of cell cultures grown under UV+photoperiod. These findings open new dimensions for feasible production of biologically active lignans and neolignans by Flax cell cultures.
authors.affiliation: Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan.
authors.forename: Sumaira
authors.initials: S
authors.lastname: Anjum
authors.affiliation: Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan. Electronic address: bhabbasi@qau.edu.pk.
authors.forename: Bilal Haider
authors.initials: BH
authors.lastname: Abbasi
authors.affiliation: Laboratoire de Biologie des Ligneux et des Grandes Cultures, INRA USC1328/Université d'Orléans, 28000 Chartres, France; Le CNAM, Conservatoire National des Arts et Métiers, CASER-SITI-CG, 2 rue Conté, 75003 Paris, France.
authors.forename: Joël
authors.initials: J
authors.lastname: Doussot
authors.affiliation: Le CNAM, Conservatoire National des Arts et Métiers, CASER-SITI-CG, 2 rue Conté, 75003 Paris, France; Université de Lyon, Laboratoire de Génie des Procédés Catalytiques (UMR 5285), CPE Lyon, 43 boulevard du 11 Novembre 1918, 69100 Villeurbanne, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Favre-Réguillon
authors.affiliation: Laboratoire de Biologie des Ligneux et des Grandes Cultures, INRA USC1328/Université d'Orléans, 28000 Chartres, France; Bioactifs et Cosmétiques, GDR 3711 COSMACTIFS, CNRS/Université d'Orléans, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Hano
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D018533:Biomass; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D005419:Flavonoids / Q000378:metabolism; D019597:Flax / Q000166:cytology / Q000378:metabolism*; D017705:Lignans / Q000096:biosynthesis*; D010636:Phenols / Q000378:metabolism; D017440:Photoperiod; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D017705:Lignans; D010636:Phenols
keywords: Antioxidant activity; Cell cultures; Lignans; Neolignans; Photoperiod regimes; UV-C