title: Selenizing Hericium erinaceus polysaccharides induces dendritic cells maturation through MAPK and NF-κB signaling pathways.
abstract: In this study, polysaccharides extracted from Hericium erinaceus were modified to obtain its nine selenium derivatives, sHEP1-sHEP9. Their structures were identified, yields and selenium contents were determined, the phenotypic and functional maturation of murine bone marrow-derived dendritic cells (DCs) and relevant mechanisms were compared taking unmodified HEP as control. The results revealed that the selenylation were successful. sHEP1, sHEP2and sHEP8treatment of DCs increased their surface expression of MHC-II and CD86 and indicated that sHEP1, sHEP2and sHEP8induced DC maturation. Furthermore, sHEP2and sHEP8also significantly decreased DCs endocytosis and significantly enhanced cytokine (IL-12 and IFN-γ) production. In line with TLR4 activation, sHEP2increased the phosphorylation of ERK, p38, and JNK, and the nuclear translocation of p-c-Jun, p-CREB, and c-Fos. sHEP2also activated NF-κB signaling, as evidenced by degradation of IκBα/β and nuclear translocation of p65 and p50. Together, these results suggest that sHEP is a strong immunostimulant.
authors.affiliation: Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health in Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Qin
authors.affiliation: Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health in Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Ren
authors.affiliation: Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health in Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China. Electronic address: yfhuangfj@163.com.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health in Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Song
authors.affiliation: Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health in Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Lin
authors.affiliation: Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health in Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health in Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
authors.forename: Yufang
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health in Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
authors.forename: Xiuqin
authors.initials: X
authors.lastname: Wu
authors.affiliation: Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health in Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
authors.forename: Fuan
authors.initials: F
authors.lastname: Qiu
authors.affiliation: Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health in Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Xiao
mesh_terms: D000818:Animals; D001487:Basidiomycota / Q000737:chemistry*; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D004705:Endocytosis / Q000187:drug effects; D007155:Immunologic Factors / Q000737:chemistry / Q000494:pharmacology; D007371:Interferon-gamma / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D051379:Mice; D009005:Monosaccharides / Q000032:analysis; D016328:NF-kappa B / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D012643:Selenium / Q000737:chemistry*
chemical_list: D007155:Immunologic Factors; D009005:Monosaccharides; D016328:NF-kappa B; D011134:Polysaccharides; D018664:Interleukin-12; D007371:Interferon-gamma; D012643:Selenium
keywords: Dendritic cell; Selenizing Hericium erinaceus polysaccharide; Signaling pathways