title: Msi1 promotes tumor progression by epithelial-to-mesenchymal transition in cervical cancer.
abstract: Musashi1 (Msi1) is an RNA-binding protein that has been reported to be a pivotal regulator in tumorigenesis and progression in several cancers. However, its function and mechanism in cervical cancer is still unknown. In this study, Msi1 expression was found elevated in cervical cancers by immunohistochemistry and correlated with poor outcomes. Then, endogenous Msi1 was silenced in cervical cancer cell lines by short hairpin RNA, and its function and mechanism were determined. The results showed that the silencing of Msi1 in SiHa and HeLa cells inhibited the cells' migratory and invasive abilities in vitro and tumor progression in vivo. Epithelial-to-mesenchymal transition (EMT) markers were down-regulated, and Wnt activity was inhibited by the silencing of Msi1. In clinical tissues, positive correlations between Msi1 and EMT markers were found. In conclusion, Msi1, a diagnostic marker and potential therapeutic target, promoted the EMT progression through activation of the Wnt signaling pathway in cervical cancers, thereby contributing to poor prognosis.
authors.affiliation: Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710004, China.
authors.forename: Pijun
authors.initials: P
authors.lastname: Gong
authors.affiliation: Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710004, China.
authors.forename: Yidong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710004, China.
authors.forename: Yane
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710004, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Gao
authors.affiliation: Department of Obstetrics and Gynecology, Xi'an Electric Power Central Hospital, Xi'an, Shaanxi, 710032, China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, Xianyang First People's Hospital, Xianyang, Shaanxi, 717200, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Qu
authors.affiliation: Department of Obstetrics and Gynecology, Xi'an Gaoxin Hospital, Xi'an, Shaanxi, 710032, China.
authors.forename: Xinxing
authors.initials: X
authors.lastname: Jin
authors.affiliation: Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710004, China. Electronic address: gaoqing201666@sina.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Gao
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002465:Cell Movement; D018572:Disease-Free Survival; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D053208:Kaplan-Meier Estimate; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D014162:Transfection; D047368:Tumor Burden; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D060449:Wnt Signaling Pathway
chemical_list: D014408:Biomarkers, Tumor; C491297:MSI1 protein, human; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins
keywords: Cervical cancer; Epithelial-to-mesenchymal transition; Msi1; Prognosis; Wnt signaling pathway