title: Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H2O2: Implications for Pharmacological Ascorbate Therapy.
abstract: Cancer cell toxicity to therapeutic H2O2 varies widely depending on cell type. Interestingly, it has been observed that different cancer cell types have varying peroxiporin expression. We hypothesize that variation in peroxiporin expression can alter cell susceptibility to therapeutic H2O2 concentrations. Here, we silence peroxiporin aquaporin-3 (AQP3) on the pancreatic cancer cell line MIA PaCa-2 and compare clonogenic survival response to the wild-type. The results showed a significantly higher surviving fraction in the clonogenic response for siAQP3 MIA PaCa-2 cells at therapeutic H2O2 doses (P < 0.05). These results suggest that peroxiporin expression is significant in modulating the susceptibility of cancer cells to ascorbate therapy.
authors.affiliation: Department of Bioengineering, University of California, Riverside, Riverside, California, United States of America.
authors.forename: Dieanira
authors.initials: D
authors.lastname: Erudaitius
authors.affiliation: Department of Neuroscience, University of California, Riverside, Riverside, California, United States of America.
authors.forename: Andrew
authors.initials: A
authors.lastname: Huang
authors.affiliation: Department of Bioengineering, University of California, Riverside, Riverside, California, United States of America.
authors.forename: Sarah
authors.initials: S
authors.lastname: Kazmi
authors.affiliation: Free Radical & Radiation Biology, Department of Radiation Oncology, University of Iowa College of Medicine, Iowa City, IA, United States of America.
authors.forename: Garry R
authors.initials: GR
authors.lastname: Buettner
authors.affiliation: Department of Bioengineering, University of California, Riverside, Riverside, California, United States of America.
authors.forename: Victor G J
authors.identifier: http://orcid.org/0000-0002-1857-8025
authors.initials: VG
authors.lastname: Rodgers
mesh_terms: D051400:Aquaporin 3 / Q000235:genetics / Q000378:metabolism*; D001205:Ascorbic Acid / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival; D020868:Gene Silencing; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007150:Immunohistochemistry; D009369:Neoplasms / Q000473:pathology*
chemical_list: D051400:Aquaporin 3; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid