title: Investigation of the Key Pharmacological Activities ofFicus racemosaand Analysis of Its Major Bioactive Polyphenols by HPLC-DAD.
abstract: Objective. Oxidative stress leads to numerous physiological disorders including infectious diseases, inflammation, and cancer. The present study was carried out to investigate antioxidant, antibacterial, and cytotoxic activity of methanol crude extract of leaves and fruits of theFicus racemosa(LCME and FCME, resp.) and to analyse its major bioactive polyphenols by HPLC-DAD.Methods. Antioxidant capacity of the extracts was evaluated by DPPH free radical scavenging, reducing power, total phenolic, total flavonoid, total tannin content assay, superoxide radical, hydroxyl radical, and hydrogen peroxide scavenging assay. Identification and quantification of bioactive polyphenols were done by HPLC-DAD method. Antibacterial activity was tested by "disc diffusion" method. Brine shrimp lethality assay was carried out to check the cytotoxic potential.Result. Both LCME and FCME showed DPPH scavenging ability and concentration dependent reducing power activity. They had phenolic content, flavonoid content, and tannin content. Both the extracts showed superoxide radical scavenging ability, hydroxyl radical scavenging ability, and hydrogen peroxide scavenging ability. HPLC analysis of LCME and FCME indicated the presence of significant amount of gallic acid along with other phenolic constituents.Conclusion. Significant amount of gallic acid along with other phenolic constituents might have played an important role in the observed antioxidant, antibacterial, and cytotoxic activity.
authors.affiliation: Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh.
authors.forename: Salma Akter
authors.initials: SA
authors.lastname: Sumi
authors.affiliation: Department of Pharmaceutical Sciences, Daniel K. Inouye College of Pharmacy, University of Hawaii at Hilo, Hilo, HI 96720, USA.
authors.forename: Md Afjalus
authors.initials: MA
authors.lastname: Siraj
authors.affiliation: Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh.
authors.forename: Amir
authors.initials: A
authors.lastname: Hossain
authors.affiliation: Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh.
authors.forename: Md Sagir
authors.initials: MS
authors.lastname: Mia
authors.affiliation: Pharmacy Department, Jahangirnagar University, Dhaka 1342, Bangladesh.
authors.forename: Seagufta
authors.initials: S
authors.lastname: Afrin
authors.affiliation: Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh.
authors.forename: Md Mustafizur
authors.identifier: 0000-0001-8585-263X
authors.initials: MM
authors.lastname: Rahman