title: Loop-Mediated Isothermal Amplification (LAMP): Emergence As an Alternative Technology for Herbal Medicine Identification.
abstract: Correct identification of medicinal plant ingredients is essential for their safe use and for the regulation of herbal drug supply chain. Loop-mediated isothermal amplification (LAMP) is a recently developed approach to identify herbal medicine species. This novel molecular biology technique enables timely and accurate testing, especially in settings where infrastructures to support polymerase chain reaction facilities are lacking. Studies that used this method have altered our view on the extent and complexity of herbal medicine identification. In this review, we give an introduction into LAMP analysis, covers the basic principles and important aspects in the development of LAMP analysis method. Then we presented a critical review of the application of LAMP-based methods in detecting and identifying raw medicinal plant materials and their processed products. We also provide a practical standard operating procedure (SOP) for the utilization of the LAMP protocol in herbal authentication, and consider the prospects of LAMP technology in the future developments of herbal medicine identification and the challenges associated with its application.
authors.affiliation: College of Forestry and Landscape Architecture, South China Agricultural University Guangzhou, China.
authors.forename: Jing-Jian
authors.initials: JJ
authors.lastname: Li
authors.affiliation: College of Pharmacy, Hubei University of Chinese Medicine Wuhan, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Xiong
authors.affiliation: Sichuan Industrial Institute of Antibiotics, Chengdu University Chengdu, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Biology and Pharmacy, Yulin Normal University Yulin, China.
authors.forename: Jun-Song
authors.initials: JS
authors.lastname: Liang
authors.affiliation: College of Biology and Pharmacy, Yulin Normal University Yulin, China.
authors.forename: Xing-Wen
authors.initials: XW
authors.lastname: Zhou
keywords: LAMP technology; detection method; drug safety; herbal medicine; species identification