title: High glucose induces N-linked glycosylation-mediated functional upregulation and overexpression of Cav3.2 T-type calcium channels in neuroendocrine-like differentiated human prostate cancer cells.
abstract: Given that Cav3.2 T-type Ca2+channels were functionally regulated by asparagine (N)-linked glycosylation, we examined effects of high glucose on the function of Cav3.2, known to regulate secretory function, in neuroendocrine-like differentiated prostate cancer LNCaP cells. High glucose accelerated the increased channel function and overexpression of Cav3.2 during neuroendocrine differentiation, the former prevented by enzymatic inhibition of N-glycosylation and cleavage of N-glycans. Hyperglycemia thus appears to induce N-linked glycosylation-mediated functional upregulation and overexpression of Cav3.2 in neuroendocrine-like differentiated prostate cancer cells.
authors.affiliation: Laboratory of Pharmacology and Pathophysiology, Faculty of Pharmacy, Kindai University (Formerly Kinki University), Higashi-Osaka 577-8502, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Fukami
authors.affiliation: Laboratory of Pharmacology and Pathophysiology, Faculty of Pharmacy, Kindai University (Formerly Kinki University), Higashi-Osaka 577-8502, Japan.
authors.forename: Erina
authors.initials: E
authors.lastname: Asano
authors.affiliation: Laboratory of Pharmacology and Pathophysiology, Faculty of Pharmacy, Kindai University (Formerly Kinki University), Higashi-Osaka 577-8502, Japan.
authors.forename: Mai
authors.initials: M
authors.lastname: Ueda
authors.affiliation: Laboratory of Pharmacology and Pathophysiology, Faculty of Pharmacy, Kindai University (Formerly Kinki University), Higashi-Osaka 577-8502, Japan.
authors.forename: Fumiko
authors.initials: F
authors.lastname: Sekiguchi
authors.affiliation: Department of Life Science, Faculty of Science and Engineering, Kindai University, Higashi-Osaka 577-8502, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Yoshida
authors.affiliation: Laboratory of Pharmacology and Pathophysiology, Faculty of Pharmacy, Kindai University (Formerly Kinki University), Higashi-Osaka 577-8502, Japan. Electronic address: kawabata@phar.kindai.ac.jp.
authors.forename: Atsufumi
authors.initials: A
authors.lastname: Kawabata
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D003994:Bucladesine / Q000494:pharmacology; D020747:Calcium Channels, T-Type / Q000096:biosynthesis*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D005947:Glucose / Q000494:pharmacology*; D006031:Glycosylation / Q000187:drug effects; D006801:Humans; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D014415:Tunicamycin / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: C479952:CACNA1H protein, human; D020747:Calcium Channels, T-Type; D014415:Tunicamycin; D003994:Bucladesine; D005947:Glucose; D015056:1-Methyl-3-isobutylxanthine
keywords: Asparagine-linked glycosylation; Ca(v)3.2 T-type calcium channel; Prostate cancer