title: The Involvement of Corin in the Progression of Diabetic Erectile Dysfunction in a Rat Model by Down-Regulating ANP /NO/cGMP Signal Pathway.
abstract: This study was aimed to analyze the potential role of Corin in the procession of diabetic ED and to explore the underlying mechanism. Diabetic ED rat model was constructed and the characteristics of diabetic ED and control rats were recorded at 4, 8, 12, and 16 weeks. qRT-PCR and Western bloting were used to detected the mRNA and protein levels. Intracellular cGMP detection was accomplished using a commercial radioimmunoassay method. Vascular endothelial cell from rat corpus cavernosum spiral artery was isolated and transfected with si- Corin to analyzed the potential role of Corin. Cell viability was assessed using crystal violet. The results showed that diabetic ED rats showed significantly higher glucose level, and lower body weight, ICP level, and ICP/MAP ratio at 12 and 16 weeks in diabetic ED rats compared with control rats. The protein levels of Corin, atrial natriuretic peptide (ANP) and eNOS, and the level of cGMP were significantly down-regulated in corpus cavernosum in diabetic ED rats, revealing the potential role of Corin in NO-associated diabetic ED. Further, studies proved that defect of Corin not only inhibited the vascular endothelial cell viability in high-glucose condition, but also suppressed ANP, eNOS, and cGMP expression in vascular endothelial cells. To sum up, Corin contributes to the progression of diabetic ED and the underlying mechanism is associated with the down-regulation of ANP /NO/cGMP signal pathway. J. Cell. Biochem. 118: 2325-2332, 2017. © 2017 Wiley Periodicals, Inc.
authors.affiliation: Department of Urology, The Third Affiliated Hospital of Nantong University, Wuxi 214041, Jiangsu, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Urology, The Third Affiliated Hospital of Nantong University, Wuxi 214041, Jiangsu, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Mi
authors.affiliation: Department of Orthopaedics and Central Laboratory, The Third Affiliated Hospital of Nantong University, Wuxi 214041, Jiangsu, China.
authors.forename: Fenglai
authors.initials: F
authors.lastname: Yuan
authors.affiliation: Department of Urology, The Third Affiliated Hospital of Nantong University, Wuxi 214041, Jiangsu, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Urology, The Third Affiliated Hospital of Nantong University, Wuxi 214041, Jiangsu, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: You
authors.affiliation: Department of Urology, The Third Affiliated Hospital of Nantong University, Wuxi 214041, Jiangsu, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Dai
authors.affiliation: Department of Urology, The Third Affiliated Hospital of Nantong University, Wuxi 214041, Jiangsu, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Ophthalmology, Nanjing Medical University Affiliated Wuxi People's Hospital, Wuxi 214041, Jiangsu, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Cao
authors.affiliation: Department of Urology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Urology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, China.
authors.forename: Boxin
authors.initials: B
authors.lastname: Xue
authors.affiliation: Department of Urology, The Third Affiliated Hospital of Nantong University, Wuxi 214041, Jiangsu, China.
authors.forename: Lijie
authors.initials: L
authors.lastname: Zhu
mesh_terms: D000818:Animals; D062186:Arterial Pressure / Q000235:genetics / Q000502:physiology; D009320:Atrial Natriuretic Factor / Q000235:genetics / Q000378:metabolism*; D001794:Blood Pressure / Q000235:genetics / Q000502:physiology; D002470:Cell Survival / Q000235:genetics / Q000502:physiology; D006152:Cyclic GMP / Q000235:genetics / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000503:physiopathology*; D007172:Erectile Dysfunction / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D009589:Nitrogen Oxides / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism*
chemical_list: D009589:Nitrogen Oxides; D009320:Atrial Natriuretic Factor; D012697:Serine Endopeptidases; C492879:corin protein, rat; D006152:Cyclic GMP
keywords: ANP/NO/cGMP; CORIN; DIABETIC ERECTILE DYSFUNCTION