title: Anti-pathogenic and probiotic attributes of Lactobacillus salivarius and Lactobacillus plantarum strains isolated from feces of Algerian infants and adults.
abstract: Sixty-seven (67) lactic acid bacteria (LAB) isolates belonging to Lactobacillus genus were isolated from human feces and tested for their auto-aggregation and cell surface hydrophobicity in order to establish their adhesion capabilities, a prerequisite for probiotic selection. Strains with the upmost auto-aggregation and cell surface hydrophobicity scores were identified by MALDI-TOF spectrometry and 16S rDNA sequencing as Lactobacillus plantarum (p25lb1 and p98lb1) and Lactobacillus salivarius (p85lb1 and p104lb1). These strains were also able to adhere to human epithelial colorectal adenocarcinoma Caco-2 cells, with percentages ranging from 4.68 to 9.59%. They displayed good survival under conditions mimicking the gastrointestinal environment and remarkably impeded adhesion and invasion of human Caco-2 by Listeria monocytogenes and Enteropathogenic Escherichia coli. It should also be noted that Lb. plantarum p98lb1 was able to reduce in vitro cholesterol concentration by about 32%, offering an additional health attribute.
authors.affiliation: Laboratoire de Microbiologie Appliquée, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, Bejaia 06000, Algeria; Univ. Lille, INRA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394 - ICV - Institut Charles Viollette, F-59000 Lille, France. Electronic address: hamzaseddik1@gmail.com.
authors.forename: Hamza
authors.initials: H
authors.lastname: Ait Seddik
authors.affiliation: Laboratoire de Microbiologie Appliquée, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, Bejaia 06000, Algeria. Electronic address: kamelea03@hotmail.com.
authors.forename: Farida
authors.initials: F
authors.lastname: Bendali
authors.affiliation: Univ. Lille, INRA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394 - ICV - Institut Charles Viollette, F-59000 Lille, France. Electronic address: Benoit.Cudennec@univ-lille1.fr.
authors.forename: Benoit
authors.initials: B
authors.lastname: Cudennec
authors.affiliation: Univ. Lille, INRA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394 - ICV - Institut Charles Viollette, F-59000 Lille, France. Electronic address: djamel.drider@univ-lille1.fr.
authors.forename: Djamel
authors.initials: D
authors.lastname: Drider
mesh_terms: D000328:Adult; D000462:Algeria; D001422:Bacterial Adhesion; D001647:Bile Acids and Salts; D018938:Caco-2 Cells; D002784:Cholesterol / Q000032:analysis; D003470:Culture Media / Q000737:chemistry; D005243:Feces / Q000382:microbiology*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007223:Infant; D048191:Lactiplantibacillus plantarum / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000069996:Ligilactobacillus salivarius / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D050296:Microbial Viability; D019936:Probiotics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D001647:Bile Acids and Salts; D003470:Culture Media; D012336:RNA, Ribosomal, 16S; D002784:Cholesterol
keywords: Anti-adhesion properties; Anti-invasion; Cholesterol lowering; Human feces; Lactobacillus; Probiotics