title: Development of Diversity Arrays Technology markers as a tool for rapid genomic assessment in Nile tilapia, Oreochromis niloticus.
abstract: The development of genomic markers is described for Nile tilapia, Oreochromis niloticus, using the Diversity Arrays Technology (DArT) genotype-by-sequencing platform. A total of 13 215 single nucleotide polymorphism (SNP) markers and 12 490 silicoDArT (dominant) markers were identified from broodstock of two selective breeding programs [Genetically Improved Farmed Tilapia (GIFT) strain from Malaysia and the Abbassa strain from Egypt]. Over 10 000 SNPs were polymorphic in either strain, and 2985 and 3087 showed strain-specific polymorphisms for the GIFT and Abbassa strains respectively. We demonstrate the potential utility of these markers for rapid genomic screening and use in breeding programs.
authors.affiliation: WorldFish, Jalan Batu Maung, 11960, Bayan Lepas, Penang, Malaysia.
authors.forename: C E
authors.identifier: http://orcid.org/0000-0002-1193-4138
authors.initials: CE
authors.lastname: Lind
authors.affiliation: Diversity Arrays Technology Pty Ltd (DArT P/L), University of Canberra, Building 3, Bruce, ACT, 2617, Australia.
authors.forename: A
authors.initials: A
authors.lastname: Kilian
authors.affiliation: WorldFish, Jalan Batu Maung, 11960, Bayan Lepas, Penang, Malaysia.|School of Biological Earth and Environmental Sciences, University College Cork, Cork, Ireland.
authors.forename: J A H
authors.initials: JAH
authors.lastname: Benzie
mesh_terms: D000818:Animals; D001947:Breeding; D023681:Cichlids / Q000235:genetics*; D005819:Genetic Markers; D023281:Genomics; D005838:Genotype; D020411:Oligonucleotide Array Sequence Analysis / Q000662:veterinary*; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D005819:Genetic Markers
keywords: 
  SNP
  ; DArTseq; aquaculture; genetically improved farmed tilapia; genotype-by-sequencing