title: Single-Cell Multiomics: Multiple Measurements from Single Cells.
abstract: Single-cell sequencing provides information that is not confounded by genotypic or phenotypic heterogeneity of bulk samples. Sequencing of one molecular type (RNA, methylated DNA or open chromatin) in a single cell, furthermore, provides insights into the cell's phenotype and links to its genotype. Nevertheless, only by taking measurements of these phenotypes and genotypes from the same single cells can such inferences be made unambiguously. In this review, we survey the first experimental approaches that assay, in parallel, multiple molecular types from the same single cell, before considering the challenges and opportunities afforded by these and future technologies.
authors.affiliation: Earlham Institute, Norwich Research Park, Norwich NR4 7UH, UK. Electronic address: Iain.Macaulay@earlham.ac.uk.
authors.forename: Iain C
authors.initials: IC
authors.lastname: Macaulay
authors.affiliation: Sanger Institute - EBI Single-Cell Genomics Centre, Wellcome Trust Sanger Institute, Hinxton CB10 1SA, UK; MRC Human Genetics Unit, MRC IGMM, University of Edinburgh, Crewe Road, Edinburgh EH4 2XU, UK. Electronic address: Chris.Ponting@igmm.ed.ac.uk.
authors.forename: Chris P
authors.initials: CP
authors.lastname: Ponting
authors.affiliation: Sanger Institute - EBI Single-Cell Genomics Centre, Wellcome Trust Sanger Institute, Hinxton CB10 1SA, UK; Department of Human Genetics, University of Leuven, KU Leuven, Leuven, 3000, Belgium. Electronic address: thierry.voet@kuleuven.be.
authors.forename: Thierry
authors.initials: T
authors.lastname: Voet
mesh_terms: D019175:DNA Methylation / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D055442:Metabolome / Q000235:genetics*; D020543:Proteome / Q000235:genetics*; D059010:Single-Cell Analysis / Q000379:methods; D059467:Transcriptome / Q000235:genetics*
chemical_list: D020543:Proteome
keywords: epigenomics; genomics; multiomics; proteomics; single cell; transcriptomics