title: Sequence, structure and function relationships in flaviviruses as assessed by evolutive aspects of its conserved non-structural protein domains.
abstract: Flaviviruses are responsible for serious diseases such as dengue, yellow fever, and zika fever. Their genomes encode a polyprotein which, after cleavage, results in three structural and seven non-structural proteins. Homologous proteins can be studied by conservation and coevolution analysis as detected in multiple sequence alignments, usually reporting positions which are strictly necessary for the structure and/or function of all members in a protein family or which are involved in a specific sub-class feature requiring the coevolution of residue sets. This study provides a complete conservation and coevolution analysis on all flaviviruses non-structural proteins, with results mapped on all well-annotated available sequences. A literature review on the residues found in the analysis enabled us to compile available information on their roles and distribution among different flaviviruses. Also, we provide the mapping of conserved and coevolved residues for all sequences currently in SwissProt as a supplementary material, so that particularities in different viruses can be easily analyzed.
authors.affiliation: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Av. Antônio Carlos, 6627, Belo Horizonte, 31270-901, Brazil. Electronic address: nelijfjr@gmail.com.
authors.forename: Néli José
authors.initials: NJ
authors.lastname: da Fonseca
authors.affiliation: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Av. Antônio Carlos, 6627, Belo Horizonte, 31270-901, Brazil. Electronic address: marceloqla@gmail.com.
authors.forename: Marcelo Querino
authors.initials: MQ
authors.lastname: Lima Afonso
authors.affiliation: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Av. Antônio Carlos, 6627, Belo Horizonte, 31270-901, Brazil. Electronic address: natanpedersolli@gmail.com.
authors.forename: Natan Gonçalves
authors.initials: NG
authors.lastname: Pedersolli
authors.affiliation: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Av. Antônio Carlos, 6627, Belo Horizonte, 31270-901, Brazil. Electronic address: lucas.carrijodeoliveira@gmail.com.
authors.forename: Lucas Carrijo
authors.initials: LC
authors.lastname: de Oliveira
authors.affiliation: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Av. Antônio Carlos, 6627, Belo Horizonte, 31270-901, Brazil. Electronic address: dsouto49@gmail.com.
authors.forename: Dhiego Souto
authors.initials: DS
authors.lastname: Andrade
authors.affiliation: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Av. Antônio Carlos, 6627, Belo Horizonte, 31270-901, Brazil. Electronic address: lbleicher@icb.ufmg.br.
authors.forename: Lucas
authors.initials: L
authors.lastname: Bleicher
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D017124:Conserved Sequence / Q000235:genetics*; D019143:Evolution, Molecular; D005416:Flavivirus / Q000235:genetics*; D016679:Genome, Viral / Q000235:genetics*; D000072417:Protein Domains / Q000235:genetics; D013329:Structure-Activity Relationship; D017361:Viral Nonstructural Proteins / Q000737:chemistry* / Q000235:genetics*
chemical_list: D017361:Viral Nonstructural Proteins
keywords: Decomposition of residue coevolution networks; Dengue; Flaviviruses; Zika