title: Biofilm formation and antimicrobial resistance genes of coagulase-negative staphylococci isolated from cows with mastitis in Argentina.
abstract: Mastitis affects the health and welfare of dairy cows worldwide. Coagulase-negative staphylococci (CNS) are known to form biofilms and are increasingly recognized as a cause of persistent bovine intramammary infections. A total of 90 CNS isolated from cows with clinical and subclinical mastitis in Argentina from 2008 to 2014 were identified by PCR-RFLP using the gap gene. Standard microtiter plate assays were used to assess CNS biofilm formation, and Staphylococcus haemolyticus species formed the strongest biofilms. The presence of biofilm-associated genes icaA, bap and aap was detected in a few isolates, while embP, fbe, atlE and eno were present in the majority of isolates. Genes encoding resistance to β-lactams were detected among the isolates; blaZ, mecA and mecC were detected in 21, 4 and 1 isolate, respectively. Resistance to macrolides and lincosamides (n = 6) was attributable to ermB, ermC, mphC or mrsA or a combination of those genes. In this study, we identified CNS species involved in mastitis and provide information about pathogenicity and antimicrobial resistance, which is essential to design efficient strategies to control mastitis caused by CNS.
authors.affiliation: Microbiology Area, Faculty of Veterinary Sciences, University of Buenos Aires, Av. Chorroarín 280, C1427CWO, Buenos Aires, Argentina.
authors.forename: Mariela E
authors.initials: ME
authors.lastname: Srednik
authors.affiliation: Faculty of Veterinary Medicine, University of Montreal, 3200 rue Sicotte, St- Hyacinthe, QC J2S 2M2, Canada.
authors.forename: Yannick D N
authors.initials: YDN
authors.lastname: Tremblay
authors.affiliation: Faculty of Veterinary Medicine, University of Montreal, 3200 rue Sicotte, St- Hyacinthe, QC J2S 2M2, Canada.
authors.forename: Josée
authors.initials: J
authors.lastname: Labrie
authors.affiliation: Faculty of Veterinary Medicine, University of Montreal, 3200 rue Sicotte, St- Hyacinthe, QC J2S 2M2, Canada.|Regroupement de Recherche Pour un lait de Qualité Optimale (Op+Lait, 3200 rue Sicotte, St-Hyacinthe, QC J2S 2M2, Canada).
authors.forename: Marie
authors.initials: M
authors.lastname: Archambault
authors.affiliation: Faculty of Veterinary Medicine, University of Montreal, 3200 rue Sicotte, St- Hyacinthe, QC J2S 2M2, Canada.|Regroupement de Recherche Pour un lait de Qualité Optimale (Op+Lait, 3200 rue Sicotte, St-Hyacinthe, QC J2S 2M2, Canada).|Canadian Bovine Mastitis and Milk Quality Research Network, 3200 rue Sicotte, St-Hyacinthe, QC J2S 2M2, Canada.
authors.forename: Mario
authors.initials: M
authors.lastname: Jacques
authors.affiliation: Instituto de Investigaciones en Producción Animal (INPA-CONICET), Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Av. Chorroarín 280, C1427CWO, Buenos Aires, Argentnina.
authors.forename: Alicia
authors.initials: A
authors.lastname: Fernández Cirelli
authors.affiliation: Microbiology Area, Faculty of Veterinary Sciences, University of Buenos Aires, Av. Chorroarín 280, C1427CWO, Buenos Aires, Argentina.
authors.forename: Elida R
authors.initials: ER
authors.lastname: Gentilini
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001118:Argentina; D018441:Biofilms / Q000254:growth & development*; D002417:Cattle; D003030:Coagulase / Q000235:genetics / Q000378:metabolism; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005260:Female; D005798:Genes, Bacterial; D008414:Mastitis, Bovine / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D012150:Polymorphism, Restriction Fragment Length; D060888:Real-Time Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013210:Staphylococcus / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*
chemical_list: D000900:Anti-Bacterial Agents; D003030:Coagulase
keywords: Argentina; antimicrobial resistance genes; biofilm; bovine mastitis; coagulase-negative staphylococci