title: NMR line-fitting quantification of polysaccharide N-acylurea-based modification in glycoconjugates of Salmonella Typhi Vi polysaccharide.
abstract: The polysaccharides modification via carbodiimide reaction is one of the most applied methods for obtaining conjugated vaccines against Salmonella enterica. However, N-acylurea carbodiimide adduct generated in the process is a critical impurity in carbohydrate-based vaccines. A quantitative NMR method was developed for assessing the N-acylurea carbodiimide adduct impurity. The procedure was based on line-fitting facilities for processing the NMR signals on complex spectra. The method showed good linearity, accuracy and precision under inter-operator variation (relative standard deviation <5%). Copyright © 2017 John Wiley & Sons, Ltd.
authors.affiliation: Finlay Institute of Vaccines, Ave 27, No. 19805, La Lisa 17100, Havana, Cuba.
authors.forename: Raine
authors.identifier: http://orcid.org/0000-0002-6987-2814
authors.initials: R
authors.lastname: Garrido
authors.affiliation: Finlay Institute of Vaccines, Ave 27, No. 19805, La Lisa 17100, Havana, Cuba.
authors.forename: Jean Pierre
authors.initials: JP
authors.lastname: Soubal
authors.affiliation: Finlay Institute of Vaccines, Ave 27, No. 19805, La Lisa 17100, Havana, Cuba.
authors.forename: Leonid
authors.initials: L
authors.lastname: Torres
authors.affiliation: Finlay Institute of Vaccines, Ave 27, No. 19805, La Lisa 17100, Havana, Cuba.
authors.forename: Ubel
authors.initials: U
authors.lastname: Ramírez
authors.affiliation: Finlay Institute of Vaccines, Ave 27, No. 19805, La Lisa 17100, Havana, Cuba.
authors.forename: Vicente
authors.initials: V
authors.lastname: Vérez
mesh_terms: D006001:Glycoconjugates / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D012485:Salmonella typhi / Q000737:chemistry*; D014508:Urea / Q000031:analogs & derivatives* / Q000737:chemistry*; D018074:Vaccines, Conjugate
chemical_list: D006001:Glycoconjugates; D011135:Polysaccharides, Bacterial; D018074:Vaccines, Conjugate; D014508:Urea
keywords: NMR; Salmonella Typhi; line-fitting; quantitative analysis