title: MFPPI - Multi FASTA ProtParam Interface.
abstract: Physico-chemical properties reflect the functional and structural characteristics of a protein. The comparative study of the physicochemical properties is important to know role of a protein in exploring its molecular evolution. A number of online and offline tools are available for calculating the physico-chemical properties of a single protein sequence. However, a tool is not available for a comparative study with graphical visualization of Multi-FASTA sequences. Hence, we describe the development and utility of MFPPI V.1.0 (a web interface developed in JAVA platform) to input each FASTA sequence from Multi-FASTA file into the ProtParam web server for the calculation of physico-chemical properties. MFPPI V.1.0 calculates different physico-chemical properties for a given set of proteins in a single run and saves the data in the MSExcel sheet. Furthermore, it provides a graphical representation of protein physico-chemical properties for analysis and visualization of data in a user-friendly manner. Therefore, the output from the analysis helps to understand compositional changes and functional relationship in evolution among organisms. We have demonstrated the utility of MFPPI V.1.0 using 17 mtATP6 protein sequences from different mammalian species. It is available for free at http://insilicogenomics.in/mfpcalc/mfppi.html.
authors.affiliation: Department of Computational Biology & Bioinformatics, Jacob School of Biotechnology & Bioengineering, Sam Higginbottom Institute of Agriculture Technology & Sciences, Allahabad-211007, Uttar Pradesh, Bharat (India).
authors.forename: Vijay Kumar
authors.initials: VK
authors.lastname: Garg
authors.affiliation: Department of Computational Biology & Bioinformatics, Jacob School of Biotechnology & Bioengineering, Sam Higginbottom Institute of Agriculture Technology & Sciences, Allahabad-211007, Uttar Pradesh, Bharat (India).
authors.forename: Himanshu
authors.initials: H
authors.lastname: Avashthi
authors.affiliation: Department of Computational Biology & Bioinformatics, Jacob School of Biotechnology & Bioengineering, Sam Higginbottom Institute of Agriculture Technology & Sciences, Allahabad-211007, Uttar Pradesh, Bharat (India).
authors.forename: Apoorv
authors.initials: A
authors.lastname: Tiwari
authors.affiliation: Department of Computational Biology & Bioinformatics, Jacob School of Biotechnology & Bioengineering, Sam Higginbottom Institute of Agriculture Technology & Sciences, Allahabad-211007, Uttar Pradesh, Bharat (India).
authors.forename: Prashant Ankur
authors.initials: PA
authors.lastname: Jain
authors.affiliation: Department of Biological Sciences, Sam Higginbottom Institute of Agriculture Technology & Sciences, Allahabad-211007, Uttar Pradesh, Bharat (India).
authors.forename: Pramod Wasudev
authors.initials: PW
authors.lastname: Ramkete
authors.affiliation: Centre for Bioinformatics, School of Biotechnology, Institute of Science, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, Bharat (India).
authors.forename: Arvind Mohan
authors.initials: AM
authors.lastname: Kayastha
authors.affiliation: Centre for Bioinformatics, School of Biotechnology, Institute of Science, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, Bharat (India).
authors.forename: Vinay Kumar
authors.initials: VK
authors.lastname: Singh