title: Development of microsatellite markers inGarcinia paucinervis(Clusiaceae), an endangered species of karst habitats.
abstract: OBJECTIVE
  Microsatellite markers were developed forGarcinia paucinervis(Clusiaceae), an endangered and endemic tree species of karst habitats, to analyze its genetic diversity and genetic structure.
  
  
  RESULTS
  Using shotgun sequencing on an Illumina MiSeq platform, a total of 22 microsatellite primer sets were characterized, of which 17 were identified as polymorphic. For these polymorphic loci, the total number of alleles per locus ranged from two to 12 across 54 individuals from three populations. The observed and expected heterozygosities ranged from 0.000 to 1.000 and from 0.000 to 0.850, respectively. No pair of loci showed significant linkage disequilibrium. Three loci in one population deviated significantly from Hardy-Weinberg equilibrium (P< 0.05). Seven loci (JSL3, JSL5, JSL22, JSL29, JSL32, JSL39, and JSL43) were successfully amplified inG. bracteata.
  
  
  CONCLUSIONS
  These markers will be useful in studies on genetic diversity and population structure ofG. paucinervis.
authors.affiliation: Key Laboratory of Beibu Gulf Environment Change and Resources Utilization of Ministry of Education, Guangxi Teachers Education University, Nanning 530001, People's Republic of China; Guangxi Key Laboratory of Earth Surface Processes and Intelligent Simulation, Guangxi Teachers Education University, Nanning 530001, People's Republic of China; School of Environment and Life Sciences, Guangxi Teachers Education University, Nanning 530001, People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Hu
authors.affiliation: Key Laboratory of Beibu Gulf Environment Change and Resources Utilization of Ministry of Education, Guangxi Teachers Education University, Nanning 530001, People's Republic of China; Guangxi Key Laboratory of Earth Surface Processes and Intelligent Simulation, Guangxi Teachers Education University, Nanning 530001, People's Republic of China; School of Environment and Life Sciences, Guangxi Teachers Education University, Nanning 530001, People's Republic of China.
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Zhang
authors.affiliation: Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards, Guangxi Institute of Chinese Medicine and Pharmaceutical Science, Nanning 530022, People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Yang
authors.affiliation: Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards, Guangxi Institute of Chinese Medicine and Pharmaceutical Science, Nanning 530022, People's Republic of China.
authors.forename: Qi-Wei
authors.initials: QW
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Beibu Gulf Environment Change and Resources Utilization of Ministry of Education, Guangxi Teachers Education University, Nanning 530001, People's Republic of China; Guangxi Key Laboratory of Earth Surface Processes and Intelligent Simulation, Guangxi Teachers Education University, Nanning 530001, People's Republic of China.
authors.forename: Chang-An
authors.initials: CA
authors.lastname: Yuan
keywords: Clusiaceae; Garcinia paucinervis; genetic diversity; microsatellite marker; population structure