title: Selective nano-sensing approach for the determination of inorganic phosphate in human urine samples.
abstract: A novel sensing approach is presented for the analysis of phosphate ions in human urine samples. The new sensor is based on the fluorescence energy transfer between a luminescent probe ([Tb-EDTA]-1) and gold nanoparticles capped with a cetyltrimethylammonium bromide (Au NPs-CTAB). The strong affinity between CTAB receptors and phosphate ions results in a highly selective assay with minimum sample preparation steps. Possible chemical interference is monitored on real-time basis via luminescence lifetime analysis. The simplicity of analysis and the competitive limit of detection in the micro-molar concentration range provide a well-suited approach for routine monitoring of phosphate ions in numerous urine samples.
authors.affiliation: Department of Chemistry, University of Central Florida, P. O. Box 25000, Orlando, FL 32816-2366, United States.
authors.forename: Alaa A
authors.initials: AA
authors.lastname: Fadhel
authors.affiliation: Department of Chemistry, University of Central Florida, P. O. Box 25000, Orlando, FL 32816-2366, United States.
authors.forename: Madeleine
authors.initials: M
authors.lastname: Johnson
authors.affiliation: Department of Chemistry, University of Central Florida, P. O. Box 25000, Orlando, FL 32816-2366, United States.
authors.forename: Khang
authors.initials: K
authors.lastname: Trieu
authors.affiliation: Department of Chemistry, University of Central Florida, P. O. Box 25000, Orlando, FL 32816-2366, United States.
authors.forename: Eda
authors.initials: E
authors.lastname: Koculi
authors.affiliation: Department of Chemistry, University of Central Florida, P. O. Box 25000, Orlando, FL 32816-2366, United States. Electronic address: andres.campiglia@ucf.edu.
authors.forename: Andres D
authors.initials: AD
authors.lastname: Campiglia
mesh_terms: D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D010710:Phosphates / Q000737:chemistry / Q000652:urine*; D016482:Urinalysis / Q000379:methods*
chemical_list: D002593:Cetrimonium Compounds; D010710:Phosphates; D006046:Gold; D000077286:Cetrimonium
keywords: FRET; Gold nanoparticles; Lanthanide sensor; Lifetime analysis; Phosphate ions; Urine analysis