title: Reduced Sampling Size with Nanopipette for Tapping-Mode Scanning Probe Electrospray Ionization Mass Spectrometry Imaging.
abstract: Mass spectrometry imaging (MSI) with ambient sampling and ionization can rapidly and easily capture the distribution of chemical components in a solid sample. Because the spatial resolution of MSI is limited by the size of the sampling area, reducing sampling size is an important goal for high resolution MSI. Here, we report the first use of a nanopipette for sampling and ionization by tapping-mode scanning probe electrospray ionization (t-SPESI). The spot size of the sampling area of a dye molecular film on a glass substrate was decreased to 6 μm on average by using a nanopipette. On the other hand, ionization efficiency increased with decreasing solvent flow rate. Our results indicate the compatibility between a reduced sampling area and the ionization efficiency using a nanopipette. MSI of micropatterns of ink on a glass and a polymer substrate were also demonstrated.
authors.affiliation: Faculty of Chemistry, Materials and Bioengineering, Kansai University.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Kohigashi
authors.affiliation: Department of Chemistry, Graduate School of Science, Osaka University.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Otsuka
authors.affiliation: Faculty of Chemistry, Materials and Bioengineering, Kansai University.
authors.forename: Ryo
authors.initials: R
authors.lastname: Shimazu
authors.affiliation: Department of Chemistry, Graduate School of Science, Osaka University.
authors.forename: Takuya
authors.initials: T
authors.lastname: Matsumoto
authors.affiliation: Faculty of Engineering, Shizuoka University.
authors.forename: Futoshi
authors.initials: F
authors.lastname: Iwata
authors.affiliation: Faculty of Chemistry, Materials and Bioengineering, Kansai University.
authors.forename: Hideya
authors.initials: H
authors.lastname: Kawasaki
authors.affiliation: Faculty of Chemistry, Materials and Bioengineering, Kansai University.
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Arakawa
keywords: ambient sampling/ionization; chemical imaging; liquid bridge; surface analysis