title: Upregulated miR-193a-3p as an oncogene in esophageal squamous cell carcinoma regulating cellular proliferation, migration and apoptosis.
abstract: MicroRNAs (miRs) are small endogenous non-coding RNAs that play a vital role in carcinogenesis. miR-193a-3p has been described in multiple cancers. However, the function of miR-193a-3p in esophageal squamous cell carcinoma (ESCC) is still unclear. To explore the role of miR-193a-3p in ESCC, reverse transcription-quantitative polymerase chain reaction was used to evaluate the expression of miR-193a-3p in 48 paired ESCC and adjacent normal tissues. In addition, the impact of miR-193a-3p on cell proliferation, migration and apoptosis were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, wound scratch assay and flow cytometry, respectively. The results revealed that miR-193a-3p was upregulated in ESCC, compared with adjacent normal tissues. Downregulation of miR-193a-3p expression using a synthesized inhibitor suppressed cell proliferation and migration, and induced cell apoptosis, indicating that miR-193a-3p could be characterized as an oncogene in ESCC. In summary, the present study demonstrated that miR-193a-3p was upregulated in ESCC, where it plays a significant role by affecting cellular proliferation, migration and apoptosis.
authors.affiliation: Department of Thoracic Surgery, Dongnan Affiliated Hospital of Xiamen University, Zhangzhou, Fujian 363000, P.R. China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Yi
authors.affiliation: Department of Thoracic Surgery, Dongnan Affiliated Hospital of Xiamen University, Zhangzhou, Fujian 363000, P.R. China.
authors.forename: Jianming
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Thoracic Surgery, Dongnan Affiliated Hospital of Xiamen University, Zhangzhou, Fujian 363000, P.R. China.
authors.forename: Changjie
authors.initials: C
authors.lastname: Jiao
authors.affiliation: Department of Thoracic Surgery, Dongnan Affiliated Hospital of Xiamen University, Zhangzhou, Fujian 363000, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Department of Thoracic Surgery, Dongnan Affiliated Hospital of Xiamen University, Zhangzhou, Fujian 363000, P.R. China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Song
authors.affiliation: Department of Thoracic Surgery, Dongnan Affiliated Hospital of Xiamen University, Zhangzhou, Fujian 363000, P.R. China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Thoracic Surgery, Dongnan Affiliated Hospital of Xiamen University, Zhangzhou, Fujian 363000, P.R. China.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Thoracic Surgery, Dongnan Affiliated Hospital of Xiamen University, Zhangzhou, Fujian 363000, P.R. China.
authors.forename: Baoli
authors.initials: B
authors.lastname: Lin
keywords: ESCC; miR-193a-3p; oncogene; upregulated