title: Study of in vitro RBCs membrane elasticity with AOD scanning optical tweezers.
abstract: The elasticity of red cell membrane is a critical physiological index for the activity of RBC. Study of the inherent mechanism for RBCs membrane elasticity transformation is attention-getting all along. This paper proposes an optimized measurement method of erythrocytes membrane shear modulus incorporating acousto-optic deflector (AOD) scanning optical tweezers system. By use of this method, both membrane shear moduli and sizes of RBCs with different in vitro times were determined. The experimental results reveal that the RBCs membrane elasticity and size decline with in vitro time extension. In addition, semi quantitative measurements of S-nitrosothiol content in blood using fluorescent spectrometry during in vitro storage show that RBCs membrane elasticity change is positively associated with the S-nitrosylation level of blood. The analysis considered that the diminished activity of the nitric oxide synthase makes the S-nitrosylation of in vitro blood weaker gradually. The main reason for worse elasticity of the in vitro RBCs is that S-nitrosylation effect of spectrin fades. These results will provide a guideline for further study of in vitro cells activity and other clinical applications.
authors.affiliation: Jiangsu Key Laboratory of Advanced Laser Materials and Devices, College of Physics & Electronic Engineering, Jiangsu Normal University, Xuzhou, Jiangsu, China.
authors.forename: Huadong
authors.initials: H
authors.lastname: Song
authors.affiliation: Jiangsu Key Laboratory of Advanced Laser Materials and Devices, College of Physics & Electronic Engineering, Jiangsu Normal University, Xuzhou, Jiangsu, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Jiangsu Key Laboratory of Advanced Laser Materials and Devices, College of Physics & Electronic Engineering, Jiangsu Normal University, Xuzhou, Jiangsu, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Jiangsu Key Laboratory of Advanced Laser Materials and Devices, College of Physics & Electronic Engineering, Jiangsu Normal University, Xuzhou, Jiangsu, China.
authors.forename: Kangzhen
authors.initials: K
authors.lastname: Tian
authors.affiliation: Jiangsu Key Laboratory of Advanced Laser Materials and Devices, College of Physics & Electronic Engineering, Jiangsu Normal University, Xuzhou, Jiangsu, China.
authors.forename: Panpan
authors.initials: P
authors.lastname: Zhu
authors.affiliation: Jiangsu Key Laboratory of Advanced Laser Materials and Devices, College of Physics & Electronic Engineering, Jiangsu Normal University, Xuzhou, Jiangsu, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Lu
authors.affiliation: Jiangsu Key Laboratory of Advanced Laser Materials and Devices, College of Physics & Electronic Engineering, Jiangsu Normal University, Xuzhou, Jiangsu, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Tang
keywords: (000.1430) Biology and medicine; (350.4855) Optical tweezers or optical manipulation