title: Draft genome sequence of an aminoglycoside-resistant RmtG-producing Pseudomonas aeruginosa ST235 isolated from a cystic fibrosis patient.
abstract: Cystic fibrosis (CF) patients are often chronically colonised or infected by non-fermenting Gram-negative bacilli, with Pseudomonas aeruginosa being the most prevalent. In this study, we report the draft genome sequence of a multidrug-resistant P. aeruginosa strain belonging to sequence type ST235, isolated from the respiratory tract of a CF patient with chronic colonisation. Whole-genome sequencing analysis revealed a 6.7Mb genome size and the presence of 12 antibiotic resistance genes, including the rmtG gene conferring high-level aminoglycoside resistance, located on the chromosome.
authors.affiliation: Center of Bacteriology, Instituto Adolfo Lutz, São Paulo, Brazil.
authors.forename: Maria Fernanda C
authors.initials: MFC
authors.lastname: Bueno
authors.affiliation: Center of Bacteriology, Instituto Adolfo Lutz, São Paulo, Brazil.
authors.forename: Gabriela Rodrigues
authors.initials: GR
authors.lastname: Francisco
authors.affiliation: Department of Clinical Analysis, School of Pharmacy, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Louise
authors.initials: L
authors.lastname: Cerdeira
authors.affiliation: Genome Investigation and Analysis Laboratory (GENIAL), Institute of Biomedical Sciences, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Susan
authors.initials: S
authors.lastname: Ienne
authors.affiliation: Genome Investigation and Analysis Laboratory (GENIAL), Institute of Biomedical Sciences, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Tiago A
authors.initials: TA
authors.lastname: Souza
authors.affiliation: Department of Clinical Analysis, School of Pharmacy, Universidade de São Paulo, São Paulo, Brazil; Department of Microbiology, Institute of Biomedical Sciences, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Nilton
authors.initials: N
authors.lastname: Lincopan
authors.affiliation: Center of Bacteriology, Instituto Adolfo Lutz, São Paulo, Brazil. Electronic address: dogarcia@yahoo.com.
authors.forename: Doroti
authors.initials: D
authors.lastname: de Oliveira Garcia
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003550:Cystic Fibrosis / Q000150:complications; D024881:Drug Resistance, Bacterial; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D006801:Humans; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D000073336:Whole Genome Sequencing
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents
keywords: Brazil; Cystic fibrosis; Pseudomonas aeruginosa; RmtG; WGS