title: Rapid Solid-Liquid Dynamic Extraction (RSLDE): a New Rapid and Greener Method for Extracting Two Steviol Glycosides (Stevioside and Rebaudioside A) from Stevia Leaves.
abstract: Stevioside and rebaudioside A are the main diterpene glycosides present in the leaves of the Stevia rebaudiana plant, which is used in the production of foods and low-calorie beverages. The difficulties associated with their extraction and purification are currently a problem for the food processing industries. The objective of this study was to develop an effective and economically viable method to obtain a high-quality product while trying to overcome the disadvantages derived from the conventional transformation processes. For this reason, extractions were carried out using a conventional maceration (CM) and a cyclically pressurized extraction known as rapid solid-liquid dynamic extraction (RSLDE) by the Naviglio extractor (NE). After only 20 min of extraction using the NE, a quantity of rebaudioside A and stevioside equal to 1197.8 and 413.6 mg/L was obtained, respectively, while for the CM, the optimum time was 90 min. From the results, it can be stated that the extraction process by NE and its subsequent purification developed in this study is a simple, economical, environmentally friendly method for producing steviol glycosides. Therefore, this method constitutes a valid alternative to conventional extraction by reducing the extraction time and the consumption of toxic solvents and favouring the use of the extracted metabolites as food additives and/or nutraceuticals. As an added value and of local interest, the experiment was carried out on stevia leaves from the Benevento area (Italy), where a high content of rebaudioside A was observed, which exhibits a sweet taste compared to stevioside, which has a significant bitter aftertaste.
authors.affiliation: Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, via Pansini, 5, 80131, Naples, Italy. mongallo@unina.it.
authors.forename: Monica
authors.initials: M
authors.lastname: Gallo
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, via Cintia, 80126, Naples, Italy.
authors.forename: Manuela
authors.initials: M
authors.lastname: Vitulano
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, via Cintia, 80126, Naples, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Andolfi
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, via Cintia, 80126, Naples, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: DellaGreca
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, via Cintia, 80126, Naples, Italy.
authors.forename: Esterina
authors.initials: E
authors.lastname: Conte
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, via Cintia, 80126, Naples, Italy.
authors.forename: Martina
authors.initials: M
authors.lastname: Ciaravolo
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, via Cintia, 80126, Naples, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Naviglio
mesh_terms: D019587:Dietary Supplements; D045786:Diterpenes, Kaurane / Q000032:analysis / Q000302:isolation & purification*; D005503:Food Additives; D005960:Glucosides / Q000032:analysis / Q000302:isolation & purification*; D006027:Glycosides / Q000032:analysis / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry; D036625:Stevia / Q000737:chemistry*; D013549:Sweetening Agents / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D045786:Diterpenes, Kaurane; D005503:Food Additives; D005960:Glucosides; D006027:Glycosides; D013549:Sweetening Agents; C012043:stevioside; C025474:rebaudioside A
keywords: Extraction; Food additives; Maceration; Nutraceuticals; RSLDE; Steviol glycosides