title: Astrovirus Diagnostics.
abstract: Various methods exist to detect an astrovirus infection. Current methods include electron microscopy (EM), cell culture, immunoassays, polymerase chain reaction (PCR) and various other molecular approaches that can be applied in the context of diagnostic or in surveillance studies. With the advent of metagenomics, novel human astrovirus (HAstV) strains have been found in immunocompromised individuals in association with central nervous system (CNS) infections. This work reviews the past and current methods for astrovirus detection and their uses in both research laboratories and for medical diagnostic purposes.
authors.affiliation: Institut Pasteur, Biology of Infection Unit, Inserm U1117, Laboratory of Pathogen Discovery, 75015 Paris, France. philippe.perot@pasteur.fr.|Institut Pasteur, Centre d'innovation et de Recherche Technologique (Citech), 75015 Paris, France. philippe.perot@pasteur.fr.
authors.forename: Philippe
authors.initials: P
authors.lastname: Pérot
authors.affiliation: Institut Pasteur, Biology of Infection Unit, Inserm U1117, Laboratory of Pathogen Discovery, 75015 Paris, France. marc.lecuit@pasteur.fr.|Paris Descartes University, Sorbonne Paris Cité, 75005, Paris, France. marc.lecuit@pasteur.fr.|Necker-Enfants Malades University Hospital, Division of Infectious Diseases and Tropical Medicine, 75015 Paris, France. marc.lecuit@pasteur.fr.
authors.forename: Marc
authors.initials: M
authors.lastname: Lecuit
authors.affiliation: Institut Pasteur, Biology of Infection Unit, Inserm U1117, Laboratory of Pathogen Discovery, 75015 Paris, France. marc.eloit@pasteur.fr.|Ecole Nationale Vétérinaire d'Alfort, 94700 Maisons-Alfort, France. marc.eloit@pasteur.fr.
authors.forename: Marc
authors.initials: M
authors.lastname: Eloit
mesh_terms: D019200:Astroviridae / Q000302:isolation & purification*; D019350:Astroviridae Infections / Q000175:diagnosis*; D003955:Diagnostic Tests, Routine / Q000379:methods*; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*
keywords: astrovirus; detection methods; molecular diagnostics; virology