title: (Re)Building a Kidney.
abstract: (Re)Building a Kidney is a National Institute of Diabetes and Digestive and Kidney Diseases-led consortium to optimize approaches for the isolation, expansion, and differentiation of appropriate kidney cell types and the integration of these cells into complex structures that replicate human kidney function. The ultimate goals of the consortium are two-fold: to develop and implement strategies forin vitroengineering of replacement kidney tissue, and to devise strategies to stimulate regeneration of nephronsin situto restore failing kidney function. Projects within the consortium will answer fundamental questions regarding human gene expression in the developing kidney, essential signaling crosstalk between distinct cell types of the developing kidney, how to derive the many cell types of the kidney through directed differentiation of human pluripotent stem cells, which bioengineering or scaffolding strategies have the most potential for kidney tissue formation, and basic parameters of the regenerative response to injury. As these projects progress, the consortium will incorporate systematic investigations in physiologic function ofin vitroandin vivodifferentiated kidney tissue, strategies for engraftment in experimental animals, and development of therapeutic approaches to activate innate reparative responses.
authors.affiliation: Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, Maine; oxburl@mmc.org idrummond@mgh.harvard.edu.
authors.forename: Leif
authors.initials: L
authors.lastname: Oxburgh
authors.affiliation: Department of Molecular Biology and.|Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, Texas.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Carroll
authors.affiliation: Department of Molecular Biology and.|Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, Texas.
authors.forename: Ondine
authors.initials: O
authors.lastname: Cleaver
authors.affiliation: Division of Kidney, Urologic, & Hematologic Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland.
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Gossett
authors.affiliation: Division of Kidney, Urologic, & Hematologic Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland.
authors.forename: Deborah K
authors.initials: DK
authors.lastname: Hoshizaki
authors.affiliation: Institute for Molecular Engineering, University of Chicago, Chicago, Illinois.
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Hubbell
authors.affiliation: Division of Nephrology, Washington University School of Medicine, St. Louis, Missouri.
authors.forename: Benjamin D
authors.initials: BD
authors.lastname: Humphreys
authors.affiliation: Division of Nephrology, Washington University School of Medicine, St. Louis, Missouri.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Jain
authors.affiliation: Department of Stem Cell Biology and Regenerative Medicine and.|Trailhead Biosystems, Inc, Cleveland, Ohio.
authors.forename: Jan
authors.initials: J
authors.lastname: Jensen
authors.affiliation: Department of Biomedical Engineering, Tufts University, Medford, Massachusetts.
authors.forename: David L
authors.initials: DL
authors.lastname: Kaplan
authors.affiliation: Epstein Department of Industrial and Systems Engineering and Information Sciences Institute, Viterbi School of Engineering and.|Department of Preventive Medicine, Keck School of Medicine, University of Southern California, Los Angeles, California.
authors.forename: Carl
authors.initials: C
authors.lastname: Kesselman
authors.affiliation: Division of Kidney, Urologic, & Hematologic Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland.
authors.forename: Christian J
authors.initials: CJ
authors.lastname: Ketchum
authors.affiliation: Cell Biology Theme, Murdoch Childrens Research Institute, Parkville, Melbourne, Australia.|Department of Pediatrics, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, Melbourne, Australia.
authors.forename: Melissa H
authors.initials: MH
authors.lastname: Little
authors.affiliation: Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine of the University of Southern California, Los Angeles, California.
authors.forename: Andrew P
authors.initials: AP
authors.lastname: McMahon
authors.affiliation: Division of Nephrology and.
authors.forename: Stuart J
authors.initials: SJ
authors.lastname: Shankland
authors.affiliation: Department of Internal Medicine, Division of Gastroenterology and.|Department of Cell and Developmental Biology and Center for Organogenesis, University of Michigan Medical School, Ann Arbor, Michigan.
authors.forename: Jason R
authors.initials: JR
authors.lastname: Spence
authors.affiliation: Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts.
authors.forename: M Todd
authors.initials: MT
authors.lastname: Valerius
authors.affiliation: Department of Surgery, Jesse Brown VA Medical Center, and.|Comprehensive Transplant Center, Department of Surgery, Northwestern University Feinberg School of Medicine, Chicago, Illinois; and.
authors.forename: Jason A
authors.initials: JA
authors.lastname: Wertheim
authors.affiliation: Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio.
authors.forename: Oliver
authors.initials: O
authors.lastname: Wessely
authors.affiliation: Department of Bioengineering, Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Nephrology Division, Massachusetts General Hospital, Charlestown, Massachusetts.
authors.forename: Iain A
authors.initials: IA
authors.lastname: Drummond
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D006801:Humans; D057026:Induced Pluripotent Stem Cells; D007668:Kidney / Q000166:cytology* / Q000254:growth & development / Q000502:physiology*; D012038:Regeneration; D046509:Tissue Culture Techniques / Q000379:methods; D054457:Tissue Scaffolds
keywords: Directed differentiation; Organogenesis; Organoid; Regeneration