title: A long noncoding RNA UCA1 promotes proliferation and predicts poor prognosis in glioma.
abstract: BACKGROUND
  Acting as a proto-oncogene, long noncoding RNAs (lncRNAs) urothelial carcinoembryonic antigen 1 (UCA1) plays a key role in the occurrence and development of several human tumors. However, the expression and biological functions of UCA1 in glioma are less known. This study discussed the expression of UCA1 in glioma and its effect on the proliferation and cell cycle of glioma cells.
  
  
  METHODS
  LncRNA UCA1 expressions in 64 glioma samples (Grade I-II in 22 cases and Grade III-IV in 42 cases, according to WHO criteria) and 10 normal brain samples were detected using real-time fluorescence quantitative PCR. On this basis, the correlations of UCA1 to clinicopathological characteristics and prognosis of glioma were assessed. Then, using qPCR, the lncRNA UCA1 expressions in glioma cell lines and astrocytes were detected. UCA1-overexpressing glioma cell lines U87 and U251 were further detected after siRNA transfection of these two cell lines, and the impact on cell proliferation and cell cycle was assessed with CCK-8 (cell counting kit-8) assay and flow cytometry method (FCM), respectively. The expression of cyclin D1, a cell cycle-related protein, was detected using Western Blot.
  
  
  RESULTS
  LncRNA UCA1 expression in the glioma samples was obviously higher as compared with the normal brain samples (P < 0.001), and the expression was correlated significantly with grading of the tumors (P < 0.05). However, lncRNA UCA1 expression was not correlated with age, gender, tumor size and KPS score (P > 0.05). After interference of UCA1 expression by siRNA transfection, the proliferation of both U251 and SHG-44 cells was inhibited (P < 0.05), with more cells arrested in G0/G1 (P < 0.05). Moreover, cyclin D1 expression was also downregulated considerably.
  
  
  CONCLUSIONS
  LncRNA UCA1 can promote the proliferation and cell cycle progression of glioma cells by upregulating cyclin D1 transcription. So UCA1 may serve as an independent prognostic indicator and a novel therapeutic target for glioma.
authors.affiliation: Department of Neurosurgery, The Affiliated 2ed Hospital of Nantong University, 6 Baby Lane North Road, Nantong, 226001, Jiangsu, China.
authors.forename: W
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, Zhejiang Cancer Hospital, 38 Guangji Road, Hangzhou, 310022, Zhejiang, China. 2226124552@qq.com.
authors.forename: C
authors.initials: C
authors.lastname: Sun
authors.affiliation: Department of Neurosurgery, The Affiliated 2ed Hospital of Nantong University, 6 Baby Lane North Road, Nantong, 226001, Jiangsu, China. cuizhimingtxt@163.com.
authors.forename: Z
authors.initials: Z
authors.lastname: Cui
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics*; D001932:Brain Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D049109:Cell Proliferation / Q000235:genetics; D019938:Cyclin D1 / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D005910:Glioma / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D000090063:Proto-Oncogene Mas; D062085:RNA, Long Noncoding / Q000096:biosynthesis*; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C530104:CCND1 protein, human; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D062085:RNA, Long Noncoding; C515159:UCA1 RNA, human; D019938:Cyclin D1
keywords: Glioma; LncRNA; Prognosis; Proliferation; UCA1