title: Production and characterization of bacterial cellulose membranes with hyaluronic acid from chicken comb.
abstract: The bacterial cellulose (BC), from Gluconacetobacter hansenii, is a biofilm with a high degree of crystallinity that can be used for therapeutic purposes and as a candidate for healing wounds. Hyaluronic acid (HA) is a constitutive polysaccharide found in the extracellular matrix and is a material used in tissue engineering and scaffolding for tissue regeneration. In this study, polymeric composites were produced in presence of hyaluronic acid isolated from chicken comb on different days of fermentation, specifically on the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation. The structural characteristics, thermal stability and molar mass of hyaluronic acid from chicken comb were evaluated. Native membrane and polymeric composites were characterized with respect to their morphology and crystallinity. The optimized process of extraction and purification of hyaluronic acid resulted in low molar mass hyaluronic acid with structural characteristics similar to the standard commercial hyaluronic acid. The results demonstrate that the polymeric composites (BC/HA-SAB) can be produced in situ. The membranes produced on the third day presented better incorporation of HA-SAB between cellulose microfiber, resulting in membranes with higher thermal stability, higher roughness and lower crystallinity. The biocompatiblily of bacterial cellulose and the importance of hyaluronic acid as a component of extracellular matrix qualify the polymeric composites as promising biomaterials for tissue engineering.
authors.affiliation: Departament of Biochemistry and Biotechnology, Londrina State University-UEL, CEP 86051-980, Londrina, PR, Brazil.
authors.forename: Sabrina Alves
authors.initials: SA
authors.lastname: de Oliveira
authors.affiliation: Departament of Chemistry, Federal University of Parana-UFPR, CEP 81531-980, Curitiba, PR, Brazil.
authors.forename: Bruno Campos
authors.initials: BC
authors.lastname: da Silva
authors.affiliation: Departament of Chemistry, Federal University of Parana-UFPR, CEP 81531-980, Curitiba, PR, Brazil.
authors.forename: Izabel Cristina
authors.initials: IC
authors.lastname: Riegel-Vidotti
authors.affiliation: Departamento of Physics, Londrina State University-UEL, CEP 86051-980, Londrina, PR, Brazil.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Urbano
authors.affiliation: Departament of Biochemistry and Biotechnology, Londrina State University-UEL, CEP 86051-980, Londrina, PR, Brazil.
authors.forename: Paula Cristina
authors.initials: PC
authors.lastname: de Sousa Faria-Tischer
authors.affiliation: Departament of Biochemistry and Biotechnology, Londrina State University-UEL, CEP 86051-980, Londrina, PR, Brazil. Electronic address: cesar.tischer@me.com.
authors.forename: Cesar Augusto
authors.initials: CA
authors.lastname: Tischer
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D002645:Chickens; D004355:Drug Stability; D041821:Gluconacetobacter / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry*; D007220:Industrial Waste; D008567:Membranes, Artificial; D008970:Molecular Weight; D013499:Surface Properties; D013696:Temperature
chemical_list: D001672:Biocompatible Materials; D007220:Industrial Waste; D008567:Membranes, Artificial; D002482:Cellulose; D006820:Hyaluronic Acid
keywords: Bacterial cellulose; Chicken comb; Hyaluronic acid; Polymeric composites