title: Hydrogen-Rich Saline Attenuates Cardiac and Hepatic Injury in Doxorubicin Rat Model by Inhibiting Inflammation and Apoptosis.
abstract: Doxorubicin (DOX) remains the most effective anticancer agent which is widely used in several adult and pediatric cancers, but its application is limited for its cardiotoxicity and hepatotoxicity. Hydrogen, as a selective antioxidant, is a promising potential therapeutic option for many diseases. In this study, we found that intraperitoneal injection of hydrogen-rich saline (H2saline) ameliorated the mortality, cardiac dysfunction, and histopathological changes caused by DOX in rats. Meanwhile, serum brain natriuretic peptide (BNP), aspartate transaminase (AST), alanine transaminase (ALT), albumin (ALB), tissue reactive oxygen species (ROS), and malondialdehyde (MDA) levels were also attenuated after H2saline treatment. What is more, we further demonstrated that H2saline treatment could inhibit cardiac and hepatic inflammation and apoptosis relative proteins expressions by western blotting test. In conclusion, our results revealed a protective effect of H2saline on DOX-induced cardiotoxicity and hepatotoxicity in rats by inhibiting inflammation and apoptosis.
authors.affiliation: Department of Cardiology, The Fourth Affiliated Hospital of Harbin Medical University, 37 Yiyuan Street, Harbin, Heilongjiang 150001, China.
authors.forename: Yunan
authors.identifier: 0000-0002-4381-4712
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Harbin, Heilongjiang 150001, China.
authors.forename: Hongxiao
authors.identifier: 0000-0002-2992-3074
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Harbin, Heilongjiang 150001, China.
authors.forename: Yanbin
authors.identifier: 0000-0002-5960-7961
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Harbin, Heilongjiang 150001, China.
authors.forename: Lin
authors.identifier: 0000-0001-8659-6121
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Harbin, Heilongjiang 150001, China.
authors.forename: Jiahui
authors.identifier: 0000-0001-9382-9341
authors.initials: J
authors.lastname: Fang
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Harbin, Heilongjiang 150001, China.
authors.forename: Wei
authors.identifier: 0000-0001-7245-4579
authors.initials: W
authors.lastname: Yang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000627:therapeutic use; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D001219:Aspartate Aminotransferases / Q000097:blood; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy*; D004317:Doxorubicin / Q000009:adverse effects*; D004452:Echocardiography; D006321:Heart / Q000187:drug effects; D006335:Heart Injuries / Q000139:chemically induced / Q000188:drug therapy*; D006859:Hydrogen / Q000737:chemistry / Q000627:therapeutic use*; D007249:Inflammation / Q000188:drug therapy; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D020097:Natriuretic Peptide, Brain / Q000378:metabolism; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D012709:Serum Albumin / Q000378:metabolism; D012965:Sodium Chloride / Q000737:chemistry / Q000627:therapeutic use*
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D017382:Reactive Oxygen Species; D012709:Serum Albumin; C495963:natriuretic peptide precursor type B, rat; D020097:Natriuretic Peptide, Brain; D012965:Sodium Chloride; D008315:Malondialdehyde; D006859:Hydrogen; D004317:Doxorubicin; D001219:Aspartate Aminotransferases