title: A novel salt-tolerant bacterial consortium for biodegradation of saline and recalcitrant petrochemical wastewater.
abstract: Treatment of a saline petrochemical wastewater with BOD5/COD ratio of less than 0.1 was investigated using a consortium consisted of three isolated salt-tolerant bacteria namely, Kocuria turfanesis, Halomonas alkaliphila and Pseudomonas balearica. Selected bacteria were isolated from petrochemical wastewater containing mineral salt mediums of 3% salinity. A lab-scale activated sludge bioreactor was used for startup in batch mode operation and after obtaining the MLSS concentration of about 3000 mg/L, the operation was changed to continuous flow mode to determine the biokinetic coefficients under different organic loading rates of 0.33-1.21 kg CODm-3d-1. The COD removal efficiency of 78.7%-61.5% was observed for treatment of real saline wastewater with a decreasing trend along with increasing the organic loading rate. In addition, results of kinetic investigation demonstrated that the yield(Y), endogenous decay coefficient (kd), maximum reaction rate (Kmax), maximum specific growth rate (μmax) and saturation constant (Ks) were 0.54 mg VSS mg COD-1, 0.014 day-1, 1.23 day-1, 0.66 day-1, and 1315 mg L-1, respectively.
authors.affiliation: Environmental Technologies Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Department of Environmental Health Engineering, School of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Electronic address: ahmadi241@gmail.com.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Ahmadi
authors.affiliation: Environmental Technologies Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Department of Environmental Health Engineering, School of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Electronic address: sahand369@yahoo.com.
authors.forename: Sahand
authors.initials: S
authors.lastname: Jorfi
authors.affiliation: School of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Electronic address: bayatiyani.hojat@gmail.com.
authors.forename: Raheleh
authors.initials: R
authors.lastname: Kujlu
authors.affiliation: Health Research Institute, Infectious and Tropical Disease Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Virology Department, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Electronic address: shokouh_gh@ymail.com.
authors.forename: Shokouh
authors.initials: S
authors.lastname: Ghafari
authors.affiliation: Department of Environmental Health Engineering, School of Health, Arak University of Medical Sciences, Arak, Iran. Electronic address: rezadarvish86@yahoo.com.
authors.forename: Reza
authors.initials: R
authors.lastname: Darvishi Cheshmeh Soltani
authors.affiliation: Environmental Technologies Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Department of Environmental Health Engineering, School of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Electronic address: n.jaafarzade@yahoo.com.
authors.forename: Nematollah
authors.initials: N
authors.lastname: Jaafarzadeh Haghighifard
mesh_terms: D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology; D012722:Sewage; D012965:Sodium Chloride; D014865:Waste Disposal, Fluid; D062065:Wastewater
chemical_list: D012722:Sewage; D012965:Sodium Chloride; D062065:Wastewater
keywords: Activated sludge bioreactor; Biokinetic coefficient; Petrochemical industry; Saline wastewater; Salt-tolerant bacteria