title: Molecular diagnostic markers ofTachysurus fulvidracoandLeiocassis longirostrisand their hybrids.
abstract: BACKGROUND
  Bagridae is an important family of catfishes and has a high market demand. Recently, more cultivable Bagridae fishes are being exploited in China, and hybridization of some species has been carried out to achieve better growth performance, favorable sex ratios and better disease resistance. Yet, these hybrids have further increased the difficulties of taxonomy identification due to morphological indistinguishableness.
  
  
  RESULTS
  In this study, the molecular identification technologies forTachysurus fulvidraco,Leiocassis longirostrissand their hybrids were successfully established by using mitochondrialCOIand nuclear ITS sequences to identify the maternal and paternal lineage, respectively.
  
  
  CONCLUSIONS
  These molecular diagnostic methods could also be used to manage breeding plans of hybrids, monitor and minimize the negative impacts of hybridization programs in aquaculture. Furthermore, our study could also provide a reference for establishing detection technique for hybrids in other groups of fishes.
authors.affiliation: Yangtze River Fisheries Research Institute, The Chinese Academy of Fisheries Sciences, No. 8, 1st Wudayuan Road, Wuhan East-lake Hi-tech Development Zone, Wuhan, 430223 China ; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, 430070 China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Liang
authors.affiliation: Yangtze River Fisheries Research Institute, The Chinese Academy of Fisheries Sciences, No. 8, 1st Wudayuan Road, Wuhan East-lake Hi-tech Development Zone, Wuhan, 430223 China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Guo
authors.affiliation: Yangtze River Fisheries Research Institute, The Chinese Academy of Fisheries Sciences, No. 8, 1st Wudayuan Road, Wuhan East-lake Hi-tech Development Zone, Wuhan, 430223 China.
authors.forename: Xiangzhong
authors.initials: X
authors.lastname: Luo
authors.affiliation: Yangtze River Fisheries Research Institute, The Chinese Academy of Fisheries Sciences, No. 8, 1st Wudayuan Road, Wuhan East-lake Hi-tech Development Zone, Wuhan, 430223 China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Li
authors.affiliation: Yangtze River Fisheries Research Institute, The Chinese Academy of Fisheries Sciences, No. 8, 1st Wudayuan Road, Wuhan East-lake Hi-tech Development Zone, Wuhan, 430223 China.
authors.forename: Guiwei
authors.initials: G
authors.lastname: Zou
keywords: COI; ITS; Leiocassis longirostris; Molecular diagnostic markers; Tachysurus fulvidraco