title: An expanded nuclear phylogenomic PCR toolkit for Sapindales.
abstract: OBJECTIVE
  We tested PCR amplification of 91 low-copy nuclear gene loci in taxa from Sapindales using primers developed forBursera simaruba(Burseraceae).
  
  
  RESULTS
  Cross-amplification of these markers among 10 taxa tested was related to their phylogenetic distance fromB. simaruba. On average, each Sapindalean taxon yielded product for 53 gene regions (range: 16-90).Arabidopsis thaliana(Brassicales), by contrast, yielded product for two. Single representatives of Anacardiaceae and Rutacaeae yielded 34 and 26 products, respectively. Twenty-six primer pairs worked for all Burseraceae species tested if highly divergentAucoumea klaineanais excluded, and eight of these amplified product in every Sapindalean taxon.
  
  
  CONCLUSIONS
  Our study demonstrates that customized primers forBurseracan amplify product in a range of Sapindalean taxa. This collection of primer pairs, therefore, is a valuable addition to the toolkit for nuclear phylogenomic analyses of Sapindales and warrants further investigation.
authors.affiliation: George Mason University, 4400 University Drive, MSN 3E1, Fairfax, Virginia 22030-4444 USA.
authors.forename: Elizabeth S
authors.initials: ES
authors.lastname: Collins
authors.affiliation: Department of Botany, National Museum of Natural History, Smithsonian Institution, MRC 166, P.O. Box 37012, Washington, D.C. 20013-7012 USA.
authors.forename: Morgan R
authors.initials: MR
authors.lastname: Gostel
authors.affiliation: George Mason University, 4400 University Drive, MSN 3E1, Fairfax, Virginia 22030-4444 USA.
authors.forename: Andrea
authors.initials: A
authors.lastname: Weeks
keywords: Anacardiaceae; Burseraceae; Rutaceae; low-copy nuclear genes; microfluidic PCR