title: Improved survival of anchorage-dependent cells in core-shell hydrogel microcapsules via co-encapsulation with cell-friendly microspheres.
abstract: In this study, we investigated the effect of intracapsular environment on the survival of anchorage-dependent cells (ADCs) encapsulated in alginate microcapsules with three different core structures, i.e. liquid, semi-liquid and microsphere-encapsulating semi-liquid core, using NIH 3T3 fibroblasts as an ADC model. For the latter, we fabricated poly (ɛ-caprolactone) microspheres and co-encapsulated them with the cells, to establish cell-substrate interactions in the capsule. The fibroblast cells co-encapsulated with the microspheres exhibited higher survival and growth than those without. This study provides a "proof of concept" for employing microspheres as a cell-friendly surface to establish intracapsular cell-substrate interactions thus prolonging the survival of encapsulated therapeutic ADCs.
authors.affiliation: a Department of Electrical and Computer Engineering , University of Illinois , Urbana , IL , USA.|b Micro and Nanotechnology Laboratory , University of Illinois , Urbana , IL , USA.
authors.forename: In-Yong
authors.initials: IY
authors.lastname: Kim
authors.affiliation: a Department of Electrical and Computer Engineering , University of Illinois , Urbana , IL , USA.|b Micro and Nanotechnology Laboratory , University of Illinois , Urbana , IL , USA.
authors.forename: Hyungsoo
authors.initials: H
authors.lastname: Choi
authors.affiliation: a Department of Electrical and Computer Engineering , University of Illinois , Urbana , IL , USA.|b Micro and Nanotechnology Laboratory , University of Illinois , Urbana , IL , USA.|c Department of Bioengineering , University of Illinois , Urbana , IL , USA.|d Department of Materials Science and Engineering , University of Illinois , Urbana , IL , USA.
authors.forename: Kyekyoon Kevin
authors.initials: KK
authors.lastname: Kim
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D002214:Capsules; D002470:Cell Survival; D018914:Cells, Immobilized / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D051379:Mice; D008863:Microspheres; D041681:NIH 3T3 Cells; D011091:Polyesters / Q000737:chemistry*
chemical_list: D000464:Alginates; D002214:Capsules; D006603:Hexuronic Acids; D011091:Polyesters; C016240:polycaprolactone; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D020723:Glucuronic Acid
keywords: Microspheres; cell encapsulation; co-encapsulation; core/shell structure; microcapsules