title: Characteristics and processing of Pol IV-dependent transcripts in Arabidopsis.
abstract: RNAi is a highly conserved machinery in eukaryotes for transcriptional or post-transcriptional silencing. In plants, RNA-directed DNA Methylation (RdDM) pathway deploys small interfering RNAs (siRNAs) to target DNA methylation. This process is initiated by the plant-specific RNA polymerase IV (Pol IV) to produce RNA precursors that are later processed into siRNAs. Until recently, characterization of Pol IV transcript had been difficult due to its presumed rapid turnover. This review summarizes five recent reports on Pol IV-dependent RNAs that explore the biogenesis and features of Pol IV transcripts, as well as alternative dicer-independent processing of Pol IV products.
authors.affiliation: Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Hsuan Yu
authors.initials: HY
authors.lastname: Kuo
authors.affiliation: Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Elise L
authors.initials: EL
authors.lastname: Jacobsen
authors.affiliation: Department of Biology, Southern University of Science and Technology, Shenzhen 518055, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Long
authors.affiliation: Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Biology, Southern University of Science and Technology, Shenzhen 518055, China; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA. Electronic address: zhaijx@sustc.edu.cn.
authors.forename: Jixian
authors.initials: J
authors.lastname: Zhai
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D018749:RNA, Plant; C499181:RNA polymerase IV, Arabidopsis; D012321:DNA-Directed RNA Polymerases