title: Synthesis and antioxidant properties of caffeic acid corn bran arabinoxylan esters.
abstract: OBJECTIVE
  As a potential of bioactive additives, corn bran arabinoxylan (CAX) was prominent in its probiotic benefits and immuno-enhancing activities. To improve the antioxidant ability of CAX, naturally occurring caffeic acid (CA) was covalently attached to CAX by esterification to generate caffeic acid corn bran arabinoxylan esters (CA-CAX) with various degrees of substitution (DS).
  
  
  METHODS
  The structure of CA-CAX was analysed by NMR, and the DS was determined by HPLC. The antioxidant activity of CA-CAX was investigated on scavenging the 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical and autoxidazing methyl linoleate, and an aqueous linolenic acid dispersion was also used as an in vitro test system to examine the inhibition effect of CA-CAX on the lipid peroxidation level after UV exposure. The ability of CA-CAX to prevent H2O2-induced oxidative damage of cell was studied by reducing MDA levels and increasing SOD and GSH-Px activities in human hepatocarcinoma (HepG2) cell.
  
  
  RESULTS
  The results certificated that CA was linked to CAX successfully with various DS. Compared with CAX, the antioxidant capacity of CA-CAX was improved significantly and enhanced with the increasing concentration and DS.
authors.affiliation: The Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.affiliation: The Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: The Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, China.
authors.forename: R
authors.initials: R
authors.lastname: Liang
authors.affiliation: The Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, China.
authors.forename: C
authors.initials: C
authors.lastname: Yang
mesh_terms: D000975:Antioxidants / Q000138:chemical synthesis* / Q000494:pharmacology; D002109:Caffeic Acids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004951:Esterification; D056945:Hep G2 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D018384:Oxidative Stress / Q000187:drug effects; D003313:Zea mays / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D002109:Caffeic Acids; D006861:Hydrogen Peroxide; C040048:caffeic acid
keywords: antioxidant; arabinoxylan; caffeic acid; cell; modify