title: CAP-Gly proteins contribute to microtubule-dependent trafficking via interactions with the C-terminal aromatic residue of α-tubulin.
abstract: In mammals, the C-terminal tyrosine residue of α-tubulin is subjected to removal/re-addition cycles resulting in tyrosinated microtubules and detyrosinated Glu-microtubules. CLIP170 and its yeast ortholog (Bik1) interact weakly with Glu-microtubules. Recently, we described a Microtubule- Rho1- and Bik1-dependent mechanism involved in Snc1 routing. Here, we further show a contribution of the yeast p150Glued ortholog (Nip100) in Snc1 trafficking. Both CLIP170 and p150Glued are CAP-Gly-containing proteins that belong to the microtubule +end-tracking protein family (known as +Tips). We discuss the +Tips-dependent role of microtubules in trafficking, the role of CAP-Gly proteins as possible molecular links between microtubules and vesicles, as well as the contribution of the Rho1-GTPase to the regulation of the +Tips repertoire and the partners associated with microtubules.
authors.affiliation: a Université Grenoble Alpes , Grenoble , France.|b Inserm , U1216 , Grenoble , France.|c CEA, BIG , Grenoble , France.
authors.forename: Annie
authors.initials: A
authors.lastname: Andrieux
authors.affiliation: a Université Grenoble Alpes , Grenoble , France.|c CEA, BIG , Grenoble , France.|d Inserm , U1038 , Grenoble , France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Aubry
authors.affiliation: a Université Grenoble Alpes , Grenoble , France.|b Inserm , U1216 , Grenoble , France.|c CEA, BIG , Grenoble , France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Boscheron
mesh_terms: D000818:Animals; D001692:Biological Transport; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D014404:Tubulin / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D008869:Microtubule-Associated Proteins; D014404:Tubulin
keywords: +Tips tyrosination; CLIP170; endosome; microtubules; p150Glued; trafficking