title: Insight into the genetic variability analysis and cultivar identification of tall fescue by using SSR markers.
abstract: BACKGROUND
  Genetic diversity of 19 forage-type and 2 turf-type cultivars of tall fescue (Festuca arundinaceaSchreb.) was revealed using SSR markers in an attempt to explore the genetic relationships among them, and examine potential use of SSR markers to identify cultivars by bulked samples.
  
  
  RESULTS
  A total of 227 clear band was scored with 14 SSR primers and out of which 201 (88.6 %) were found polymorphic. The percentage of polymorphic bands (PPB) per primer pair varied from 62.5 to 100 % with an average of 86.9 %. The polymorphism information content (PIC) value ranged from 0.116 to 0.347 with an average of 0.257 and the highest PIC value (0.347) was noticed for primer NFA040 followed by NFA113 (0.346) whereas the highest discriminating power (D) of 1 was shown in NFA037 and LMgSSR02-01C. A Neighbor-joining dendrogram and the principal component analysis identified six major clusters and grouped the cultivars in agreement with their breeding histories. STRUCTURE analysis divided these cultivars into 3 sub-clades which correspond to distance based groupings.
  
  
  CONCLUSIONS
  These findings indicates that SSR markers by bulking strategy are a useful tool to measure genetic diversity among tall fescue cultivars and could be used to supplement morphological data for plant variety protection.
authors.affiliation: Animal Science and Technology College, Sichuan Agricultural University, Chengdu, 611130 China.
authors.forename: Kaixin
authors.initials: K
authors.lastname: Fu
authors.affiliation: Animal Science and Technology College, Sichuan Agricultural University, Chengdu, 611130 China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Animal Science and Technology College, Sichuan Agricultural University, Chengdu, 611130 China.
authors.forename: Xinquan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Chongqing Municipal Institute of Animal Husbandry, Chongqing, 400039 China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Animal Science and Technology College, Sichuan Agricultural University, Chengdu, 611130 China.
authors.forename: Wendan
authors.initials: W
authors.lastname: Wu
authors.affiliation: Sichuan Academy of Grassland Science, Chengdu, 611731 China.
authors.forename: Daxu
authors.initials: D
authors.lastname: Li
authors.affiliation: Animal Science and Technology College, Sichuan Agricultural University, Chengdu, 611130 China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Animal Science and Technology College, Sichuan Agricultural University, Chengdu, 611130 China.
authors.forename: Linkai
authors.initials: L
authors.lastname: Huang
authors.affiliation: Animal Science and Technology College, Sichuan Agricultural University, Chengdu, 611130 China.
authors.forename: Ming
authors.initials: M
authors.lastname: Sun
authors.affiliation: Sichuan Academy of Grassland Science, Chengdu, 611731 China.
authors.forename: Shiqie
authors.initials: S
authors.lastname: Bai
authors.affiliation: Animal Science and Technology College, Sichuan Agricultural University, Chengdu, 611130 China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Ma
mesh_terms: D018744:DNA, Plant / Q000235:genetics; D031751:Festuca / Q000145:classification / Q000235:genetics*; D005819:Genetic Markers; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers
keywords: Cultivar identification; Genetic diversity; Neighbor joining; Population structure; Principal component analysis; SSR markers; Tall fescue