title: Montagnuphilones A-G, Azaphilones from Montagnulaceae sp. DM0194, a Fungal Endophyte of Submerged Roots of Persicaria amphibia.
abstract: Seven azaphilones, montagnuphilones A-G (1-7), together with previously known azaphilones 8-11, were encountered in Montagnulaceae sp. DM0194, an endophytic fungus isolated from submerged roots of Persicaria amphibia. The structures of 1-7 were elucidated on the basis of their MS and NMR spectroscopic analysis. Compounds 1-8 were evaluated for their cytotoxicity and ability to inhibit nitric oxide (NO) production in lipopolysaccharide-activated RAW264.7 macrophage cells. Among these, none were found to be cytotoxic to RAW264.7 cells up to 100.0 μM, but 8, 5, and 2 showed NO inhibitory activity with IC50values of 9.2 ± 0.9, 25.5 ± 1.1, and 39.6 ± 1.8 μM, respectively.
authors.affiliation: Natural Products Center, School of Natural Resources and the Environment, College of Agriculture and Life Sciences, University of Arizona , 250 E. Valencia Road, Tucson, Arizona 85706, United States.|State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University , 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China.
authors.forename: Jian-Guang
authors.initials: JG
authors.lastname: Luo
authors.affiliation: Natural Products Center, School of Natural Resources and the Environment, College of Agriculture and Life Sciences, University of Arizona , 250 E. Valencia Road, Tucson, Arizona 85706, United States.
authors.forename: Ya-Ming
authors.initials: YM
authors.lastname: Xu
authors.affiliation: School of Plant Sciences, College of Agriculture and Life Sciences, University of Arizona , Tucson, Arizona 85721, United States.
authors.forename: Dustin C
authors.initials: DC
authors.lastname: Sandberg
authors.affiliation: School of Plant Sciences, College of Agriculture and Life Sciences, University of Arizona , Tucson, Arizona 85721, United States.
authors.forename: A Elizabeth
authors.initials: AE
authors.lastname: Arnold
authors.affiliation: Natural Products Center, School of Natural Resources and the Environment, College of Agriculture and Life Sciences, University of Arizona , 250 E. Valencia Road, Tucson, Arizona 85706, United States.
authors.forename: A A Leslie
authors.identifier: 0000-0001-9663-3600
authors.initials: AA
authors.lastname: Gunatilaka
mesh_terms: D000663:Amphibians / Q000382:microbiology*; D000818:Animals; D001203:Ascomycota / Q000737:chemistry*; D001578:Benzopyrans / Q000737:chemistry* / Q000302:isolation & purification; D060026:Endophytes / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D008264:Macrophages / Q000737:chemistry / Q000187:drug effects*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009569:Nitric Oxide / Q000096:biosynthesis* / Q000737:chemistry; D010860:Pigments, Biological / Q000737:chemistry* / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry*
chemical_list: D001578:Benzopyrans; D008070:Lipopolysaccharides; D010860:Pigments, Biological; C494154:azaphilone; D009569:Nitric Oxide