title: Functional Characterization of the N-Terminal C2 Domain fromArabidopsis thalianaPhospholipase Dαand Dβ.
abstract: Most of plant phospholipases D (PLD) exhibit a C2-lipid binding domain of around 130 amino acid residues at their N-terminal region, involved in their Ca2+-dependent membrane binding. In this study, we expressed and partially purified catalytically active PLDαfromArabidopsis thaliana(AtPLDα) in the yeastPichia pastoris. The N-terminal amino acid sequence of the recombinant AtPLDαwas found to be NVEETIGV and thus to lack the first 35 amino acid belonging to the C2 domain, as found in other recombinant or plant purified PLDs. To investigate the impact of such a cleavage on the functionality of C2 domains, we expressed, inE. coli, purified, and refolded the mature-like form of the C2 domain of the AtPLDαalong with its equivalent C2 domain of the AtPLDβ, for the sake of comparison. Using Förster Resonance Energy Transfer and dot-blot assays, both C2 domains were shown to bind phosphatidylglycerol in a Ca2+-independent manner while phosphatidic acid and phosphatidylserine binding were found to be enhanced in the presence of Ca2+. Amino acid sequence alignment and molecular modeling of both C2 domains with known C2 domain structures revealed the presence of a novel Ca2+-binding site within the C2 domain of AtPLDα.
authors.affiliation: Institut de Chimie et de Biochimie Moléculaires et Supramoléculaires (ICBMS), Univ Lyon, Université Lyon 1, UMR 5246 CNRS, Métabolisme, Enzymes et Mécanismes Moléculaires (MEM), F-69622 Villeurbanne cedex, France.
authors.forename: Renaud
authors.identifier: 0000-0002-1686-9806
authors.initials: R
authors.lastname: Rahier
authors.affiliation: Institut de Chimie et de Biochimie Moléculaires et Supramoléculaires (ICBMS), Univ Lyon, Université Lyon 1, UMR 5246 CNRS, Métabolisme, Enzymes et Mécanismes Moléculaires (MEM), F-69622 Villeurbanne cedex, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Noiriel
authors.affiliation: Institut de Chimie et de Biochimie Moléculaires et Supramoléculaires (ICBMS), Univ Lyon, Université Lyon 1, UMR 5246 CNRS, Métabolisme, Enzymes et Mécanismes Moléculaires (MEM), F-69622 Villeurbanne cedex, France.
authors.forename: Abdelkarim
authors.identifier: 0000-0002-8020-4294
authors.initials: A
authors.lastname: Abousalham
mesh_terms: D017360:Arabidopsis / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000378:metabolism; D002118:Calcium / Q000737:chemistry* / Q000378:metabolism; D008563:Membrane Lipids / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D010739:Phospholipase D / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D000072417:Protein Domains
chemical_list: D029681:Arabidopsis Proteins; D008563:Membrane Lipids; D010739:Phospholipase D; D002118:Calcium