title: Multilayered particle-packed column: Evaluation and comparison with monolithic and core-shell particle columns for the determination of red azo dyes in Sequential Injection Chromatography.
abstract: A recently presented new type of "multilayered" organic-inorganic hybrid silica particle packed column YMC-Triart C18(50 mm × 4.6 mm, 5 μm) was used for the development of a sequential injection chromatography method for determination of five azo dyes (Sudan I, Sudan II, Sudan III, Sudan orange G, and para red) in selected food seasonings. The use of a novel sorbent brings attractive features, reduced backpressure, and broader chemical stability together with high separation performance, which are discussed and compared with that of three types of columns typically used in medium-pressure flow chromatography techniques (classic monolithic, narrow monolithic, and core-shell particle columns). The separation was performed in gradient elution mode created by the zone mixing of two mobile phases (acetonitrile/water 90:10, 1.5 mL + acetonitrile/water 100:0, 2.3 mL) at a flow rate of 0.60 mL/min and time of analysis <9.5 min. The spectrophotometric detection wavelengths were set to 400, 480, and 500 nm. The high performance of the developed method with multilayered particle column was well documented and the results indicate a broad capability of sequential injection chromatography.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Hradec Králové, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Chocholouš
authors.affiliation: Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal.
authors.forename: Renato
authors.initials: R
authors.lastname: Gil
authors.affiliation: INQUISUR(UNS-CONICET), Department of Chemistry, Universidad Nacional del Sur, Bahía Blanca, Argentina.
authors.forename: Carolina C
authors.initials: CC
authors.lastname: Acebal
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Hradec Králové, Czech Republic.
authors.forename: Viktor
authors.initials: V
authors.lastname: Kubala
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Hradec Králové, Czech Republic.
authors.forename: Dalibor
authors.initials: D
authors.lastname: Šatínský
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Hradec Králové, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Solich
keywords: azo dyes; columns comparison; multilayered particle column; sequential injection chromatography