title: TRIP13 is expressed in colorectal cancer and promotes cancer cell invasion.
abstract: Thyroid hormone receptor interactor 13 (TRIP13) is a member of the ATPases associated with various cellular activities family of proteins and is highly conserved in a wide range of species. Recent studies have demonstrated that TRIP13 is critical for the inactivation of the spindle assembly checkpoint and is associated with the progression of certain cancers. In the present study, the role of TRIP13 in colorectal cancer (CRC) was examined. Reverse transcription-quantitative polymerase chain reaction analysis revealed that TRIP13 messenger RNA was highly expressed in multiple CRC tissues. The depletion of TRIP13 in CRC cells suppressed cell proliferation, migration and invasion. To determine whether the catalytic activity of TRIP13 was critical for cancer progression, an inactive mutant of TRIP13 was expressed in CRC cells. The invasion of cancer cells that expressed the mutant TRIP13 was significantly reduced compared with that of the wild type TRIP13-expressing cancer cells. These results indicate that TRIP13 could be a potential target for CRC treatment.
authors.affiliation: Department of Surgical Oncology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kurita
authors.affiliation: Division of Cancer Biology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
authors.forename: Masao
authors.initials: M
authors.lastname: Maeda
authors.affiliation: Division of Cancer Biology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
authors.forename: Mohammed A
authors.initials: MA
authors.lastname: Mansour
authors.affiliation: Department of Surgical Oncology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Kokuryo
authors.affiliation: Department of Surgical Oncology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Uehara
authors.affiliation: Department of Surgical Oncology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Yokoyama
authors.affiliation: Department of Surgical Oncology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Nagino
authors.affiliation: Division of Cancer Biology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
authors.forename: Michinari
authors.initials: M
authors.lastname: Hamaguchi
authors.affiliation: Division of Cancer Biology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Senga
keywords: AAA+; TRIP13; colorectal cancer; invasion; migration