title: ATP Binding Cassette Transporter ABCA7 Regulates NKT Cell Development and Function by Controlling CD1d Expression and Lipid Raft Content.
abstract: ABCA7 is an ABC transporter expressed on the plasma membrane, and actively exports phospholipid complexes from the cytoplasmic to the exocytoplasmic leaflet of membranes. Invariant NKT (iNKT) cells are a subpopulation of T lymphocytes that recognize glycolipid antigens in the context of CD1d-mediated antigen presentation. In this study, we demonstrate that ABCA7 regulates the development of NKT cells in a cell-extrinsic manner. We found that in Abca7-/-mice there is reduced expression of CD1d accompanied by an alteration in lipid raft content on the plasma membrane of thymocytes and antigen presenting cells. Together, these alterations caused by absence of ABCA7 negatively affect NKT cell development and function.
authors.affiliation: La Jolla Institute for Allergy and Immunology, La Jolla, CA, USA.
authors.forename: Heba N
authors.initials: HN
authors.lastname: Nowyhed
authors.affiliation: La Jolla Institute for Allergy and Immunology, La Jolla, CA, USA.
authors.forename: Shilpi
authors.initials: S
authors.lastname: Chandra
authors.affiliation: La Jolla Institute for Allergy and Immunology, La Jolla, CA, USA.
authors.forename: William
authors.initials: W
authors.lastname: Kiosses
authors.affiliation: La Jolla Institute for Allergy and Immunology, La Jolla, CA, USA.
authors.forename: Paola
authors.initials: P
authors.lastname: Marcovecchio
authors.affiliation: La Jolla Institute for Allergy and Immunology, La Jolla, CA, USA.
authors.forename: Farah
authors.initials: F
authors.lastname: Andary
authors.affiliation: La Jolla Institute for Allergy and Immunology, La Jolla, CA, USA.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Lipid Metabolism Unit and Center for Computational and Integrative Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
authors.forename: Michael L
authors.initials: ML
authors.lastname: Fitzgerald
authors.affiliation: La Jolla Institute for Allergy and Immunology, La Jolla, CA, USA.
authors.forename: Mitchell
authors.initials: M
authors.lastname: Kronenberg
authors.affiliation: La Jolla Institute for Allergy and Immunology, La Jolla, CA, USA.
authors.forename: Catherine C
authors.initials: CC
authors.lastname: Hedrick
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D055610:Antigens, CD1d / Q000378:metabolism*; D005260:Female; D008297:Male; D021962:Membrane Microdomains / Q000276:immunology / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D055611:Natural Killer T-Cells / Q000276:immunology / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; C485856:Abca7 protein, mouse; D055610:Antigens, CD1d; C584692:CD1d antigen, mouse