title: Exploiting cell cycle inhibitor genes of the KRP family to control root-knot nematode induced feeding sites in plants.
abstract: Cell cycle control in galls provoked by root-knot nematodes involves the activity of inhibitor genes like the Arabidopsis ICK/KRP members. Ectopic KRP1, KRP2 and KRP4 expression resulted in decreased gall size by inhibiting mitotic activity, whereas KRP6 induces mitosis in galls. Herein, we investigate the role of KRP3, KRP5 and KRP7 during gall development and compared their role with previously studied members of this class of cell cycle inhibitors. Overexpression of KRP3 and KRP7 culminated in undersized giant cells, with KRP3OEgalls presenting peculiar elongated giant cells. Nuclei in KRP3OEand KRP5OElines presented a convoluted and apparently connected phenotype. This appearance may be associated with the punctuated protein nuclear localization driven by specific common motifs. As well, ectopic expression of KRP3OEand KRP5OEaffected nematode development and offspring. Decreased mitotic activity in galls of KRP3OEand KRP7OElines led to a reduced gall size which presented distinct shapes - from more elongated like in the KRP3OEline to small rounded like in the KRP7OEline. Results presented strongly support the idea that induced expression of cell cycle inhibitors such as KRP3 and KRP7 in galls can be envisaged as a conceivable strategy for nematode feeding site control in crop species attacked by phytopathogenic nematodes.
authors.affiliation: INRA, University of Nice Sophia Antipolis, CNRS, UMR 1355-7254 Institut Sophia Agrobiotech, 06900, Sophia Antipolis, France.|Embrapa Genetic Resources and Biotechnology, Parque Estação Biológica, PqEB - Av. W5 Norte, Caixa Postal 02372, CEP 70770-917, Brasília, DF, Brazil.
authors.forename: Roberta Ramos
authors.initials: RR
authors.lastname: Coelho
authors.affiliation: INRA, University of Nice Sophia Antipolis, CNRS, UMR 1355-7254 Institut Sophia Agrobiotech, 06900, Sophia Antipolis, France.|NemaLab/ICAAM - Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Universidade de Évora, Núcleo da Mitra, Ap., 94,7002-554, Évora, Portugal.
authors.forename: Paulo
authors.initials: P
authors.lastname: Vieira
authors.affiliation: INRA, University of Nice Sophia Antipolis, CNRS, UMR 1355-7254 Institut Sophia Agrobiotech, 06900, Sophia Antipolis, France.|Embrapa Genetic Resources and Biotechnology, Parque Estação Biológica, PqEB - Av. W5 Norte, Caixa Postal 02372, CEP 70770-917, Brasília, DF, Brazil.
authors.forename: José Dijair
authors.identifier: http://orcid.org/0000-0002-4992-0698
authors.initials: JD
authors.lastname: Antonino de Souza Júnior
authors.affiliation: INRA, University of Nice Sophia Antipolis, CNRS, UMR 1355-7254 Institut Sophia Agrobiotech, 06900, Sophia Antipolis, France.
authors.forename: Cristina
authors.initials: C
authors.lastname: Martin-Jimenez
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052, Gent, Belgium.|Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052, Gent, Belgium.
authors.forename: Lieven
authors.initials: L
authors.lastname: De Veylder
authors.affiliation: Université de Nice Sophia Antipolis, 06103, Nice, France.|Centre National de la Recherche Scientifique (CNRS), Institut de Pharmacologie Moléculaire et Cellulaire, UMR 7275, 06560, Valbonne, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Cazareth
authors.affiliation: INRA, University of Nice Sophia Antipolis, CNRS, UMR 1355-7254 Institut Sophia Agrobiotech, 06900, Sophia Antipolis, France.
authors.forename: Gilbert
authors.initials: G
authors.lastname: Engler
authors.affiliation: Embrapa Genetic Resources and Biotechnology, Parque Estação Biológica, PqEB - Av. W5 Norte, Caixa Postal 02372, CEP 70770-917, Brasília, DF, Brazil.
authors.forename: Maria Fatima
authors.initials: MF
authors.lastname: Grossi-de-Sa
authors.affiliation: INRA, University of Nice Sophia Antipolis, CNRS, UMR 1355-7254 Institut Sophia Agrobiotech, 06900, Sophia Antipolis, France.
authors.forename: Janice
authors.identifier: http://orcid.org/0000-0002-8312-672X
authors.initials: J
authors.lastname: de Almeida Engler
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000166:cytology* / Q000235:genetics / Q000469:parasitology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics; D018797:Cell Cycle Proteins; D002467:Cell Nucleus / Q000235:genetics; D050756:Cyclin-Dependent Kinase Inhibitor Proteins / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D006790:Host-Parasite Interactions / Q000235:genetics; D007976:Leupeptins / Q000494:pharmacology; D018517:Plant Roots / Q000378:metabolism / Q000469:parasitology; D010941:Plant Tumors / Q000235:genetics; D030821:Plants, Genetically Modified; D011003:Ploidies; D011401:Promoter Regions, Genetic; D014431:Tylenchoidea / Q000472:pathogenicity* / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; C000625627:KRP3 protein, Arabidopsis; C584094:KRP5 protein, Arabidopsis; C587774:KRP7 protein, Arabidopsis; D007976:Leupeptins; C072553:benzyloxycarbonylleucyl-leucyl-leucine aldehyde
keywords: Arabidopsis; Meloidogyne incognita; giant cells