title: Cytotoxic activity of genistein-8-C-glucoside formLupinus luteusL. and genistein against human SK-OV-3 ovarian carcinoma cell line.
abstract: Genistein belongs to isoflavones, which are a subclass of flavonoids, a large group of polyphenolic compounds widely distributed in plants. Numerous in vitro studies suggest that isoflavones, particularly genistein, have both chemopreventive and chemotherapeutic potential in multiple tumor types. However, the molecular and cellular mechanisms of genistein effects on human ovarian cancer cells are still little known. In the present study, we investigated anticancer activity of genistein and its natural glucoside, genistein-8-C-glucoside isolated from flowers ofLupinus luteusL. We examined the effects of the two isoflavones alone or in combination on cultured human SK-OV-3 ovarian carcinoma cells. The cells were exposed to genistein and genistein-8-C-glucoside at various concentrations (1-90 µM) for 24 and 48 h. The cytotoxic and apoptotic properties of compounds were studied by the colorimetric 3-[4,5-2-yl]-2-5-diphenyltetrazolium bromide assay and the acridine orange/ethidium bromide staining technique. The morphological features of SK-OV-3 cells were examined by Nomarski differential interference contrast combined with a confocal laser scanning microscope. The level of ROS was evaluated with fluorescence probes: dichlorofluorescein-diacetate by flow cytometry. Changes in mitochondrial membrane potential were determined using 5,5,6,6-tetrachloro-1,1,3,3-tetraethylbenzimidazolcarbocyanine iodide. Genistein-treatment and genistein-8-C-glucoside-treatment resulted in the inhibition of cell proliferation, induction of apoptotic cell death and loss of mitochondrial membrane potential. The present data provide the first evidence in vitro that genistein-8-C-glucoside and combination genistein-genistein-8-C-glucoside could be a potential chemotherapeutic candidate for ovarian cancer therapy.
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., Lodz, 90-236 Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Antosiak
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., Lodz, 90-236 Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Milowska
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., Lodz, 90-236 Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Maczynska
authors.affiliation: Department of Industrial Microbiology and Biotechnology, University of Lodz, 12/16 Banacha St., Lodz, 90-237 Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Rozalska
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., Lodz, 90-236 Poland.
authors.forename: Teresa
authors.initials: T
authors.lastname: Gabryelak
keywords: Apoptosis; Cytotoxicity; Genistein; Genistein-8-C-glucoside; Ovarian cancer