title: LC-MS/MS determination of d-mannose in human serum as a potential cancer biomarker.
abstract: Several metabolites in human serum have been identified as potential cancer biomarkers for early detection. This study focuses on the LC-MS/MS method development and validation of d-mannose in human serum. Surrogate blank serum, coupled with stable isotope d-mannose-13C6, as internal standard, was used for generating standard curves ranging from 1 to 50μg/mL. Separation was achieved by an Agilent 1200 series HPLC equipped with a SUPELCOGELTM Pb, 6% Crosslinked column with HPLC water as a mobile phase at flow rate of 0.5mL/min at 80°C. Mass detection was performed under negative ionization electrospray. Inter- and intra-day accuracy and precision were <2%. The extraction recovery and matrix effect were 104.1%-105.5% and 97.0%-100.0%, respectively. This method was successfully applied for the quantification of d-mannose in the serum samples of 320 esophageal cancer patients and 323 healthy volunteers. We report a simple, specific and reproducible LC-MS/MS method for the quantification of d-mannose in human serum as a potential cancer biomarker.
authors.affiliation: Department of Pharmaceutical and Environmental Health Sciences, Texas Southern University, Houston, TX 77004, United States.
authors.forename: Lyndsey
authors.initials: L
authors.lastname: White
authors.affiliation: Department of Pharmaceutical and Environmental Health Sciences, Texas Southern University, Houston, TX 77004, United States.
authors.forename: Jing
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Pharmaceutical and Environmental Health Sciences, Texas Southern University, Houston, TX 77004, United States.
authors.forename: Su
authors.initials: S
authors.lastname: Liang
authors.affiliation: Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, United States.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Sanchez-Espiridion
authors.affiliation: Department of Pharmaceutical and Environmental Health Sciences, Texas Southern University, Houston, TX 77004, United States. Electronic address: liang_dx@tsu.edu.
authors.forename: Dong
authors.initials: D
authors.lastname: Liang
mesh_terms: D014408:Biomarkers, Tumor / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D008358:Mannose / Q000737:chemistry*; D009369:Neoplasms / Q000175:diagnosis*; D015203:Reproducibility of Results; D044967:Serum / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor; D008358:Mannose
keywords: Biomarker; Esophageal cancer; LC–MS/MS; Mannose; Serum