title: Identification of the Relationship between Oil Body Morphology and Oil Content by Microstructure Comparison Combining with QTL Analysis inBrassica napus.
abstract: Oil bodies (OBs) are relatively simple but very important organelles comprising a matrix of triacylglycerol (TAG) surrounded by a phospholipid monolayer embedded and covered with unique proteins. The OB structure inBrassica napuswith different oil content and the relationship between the oil content and the OB structure needs to be better understood. In this paper, the characteristics of OBs in the embryo of a series ofB. napusmaterials with different oil content ranging from 34% to over 60% were studied. The results indicated that the OB size was significantly positively correlated with the oil content but was significantly negatively correlated with the glucosinolates and the protein content. Many genes associated with TAG synthesis, OB-membrane proteins, and the cell progress regulatory pathway were identified in the confidence interval of co-located QTLs for oil content, fatty acid (FA) compositions, and protein content. Our results suggested that the morphology of OBs might be directly controlled by the genes associated with OB-membrane proteins and indirectly controlled by the genes associated with TAG synthesis and cell progress regulatory pathway.
authors.affiliation: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and TechnologyWuhan, China; Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang Normal UniversityHuanggang, China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Gu
authors.affiliation: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Chao
authors.affiliation: Hybrid Rapeseed Research Center of Shaanxi Province, Shaanxi Rapeseed Branch of National Centre for Oil Crops Genetic Improvement Yangling, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Hybrid Rapeseed Research Center of Shaanxi Province, Shaanxi Rapeseed Branch of National Centre for Oil Crops Genetic Improvement Yangling, China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Hybrid Rapeseed Research Center of Shaanxi Province, Shaanxi Rapeseed Branch of National Centre for Oil Crops Genetic Improvement Yangling, China.
authors.forename: Dianrong
authors.initials: D
authors.lastname: Li
authors.affiliation: Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang Normal University Huanggang, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xiang
authors.affiliation: Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang Normal University Huanggang, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Gan
authors.affiliation: Oil Crops Research Institute, Chinese Academy of Agricultural Sciences Wuhan, China.
authors.forename: Guangyuan
authors.initials: G
authors.lastname: Lu
authors.affiliation: Oil Crops Research Institute, Chinese Academy of Agricultural Sciences Wuhan, China.
authors.forename: Xuekun
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute of Biotechnology, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Long
authors.affiliation: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and TechnologyWuhan, China; Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang Normal UniversityHuanggang, China.
authors.forename: Maoteng
authors.initials: M
authors.lastname: Li
keywords: Brassica napus; QTL; fatty acids; map alignment; oil body; oil content