title: MicroRNA-142-5p Overexpression Inhibits Cell Growth and Induces Apoptosis by Regulating FOXO in Hepatocellular Carcinoma Cells.
abstract: Abnormal expression of microRNA (miR)-142-5p has been reported in hepatocellular carcinoma (HCC). However, little information is available regarding the functional role of miR-142-5p in HCC. We aimed to explore the effects of miR-142-5p aberrant expression on HCC cell growth and cell apoptosis, as well as the underlying mechanism. Human HCC cell lines HepG2 and SMMC-7721 cells were transfected with miR-142-5p mimic, inhibitor, or a corresponding negative control. Cell viability, cell cycle distribution, and cell apoptosis were then analyzed. In addition, protein expression of Forkhead box, class O (FOXO) 1 and 3, a Bcl-2-interacting mediator of cell death (Bim), procaspase 3, and activated caspase 3 was measured. After transfection with miR-142-5p inhibitor, FOXO1 and FOXO3 were overexpressed, and then the cell viability and cell apoptosis were determined again. The relative cell viability in both HepG2 and SMMC-7721 cells was significantly reduced by miR-142-5p overexpression (p < 0.05). miR-142-5p overexpression displayed a significant blockage at the G1/S transition and significantly increased the percentages of G0/G1 phase. Moreover, the results showed that miR-142-5p overexpression significantly induced cell apoptosis and statistically elevated the protein expression levels of FOXO1, FOXO3, Bim, procaspase 3, and activated caspase 3. However, the cells transfected with miR-142-5p inhibitor showed contrary results. Additionally, the effects of miR-142-5p inhibitor on cell viability and apoptosis were reversed by overexpression of FOXO. In conclusion, our results suggest that miR-142-5p overexpression shows an important protective role in HCC by inhibiting cell growth and inducing apoptosis. These effects might be by regulating FOXO expression in HCC cells.
authors.affiliation: Department of Ultrasound, Xuzhou Central HospitalXuzhou, JiangsuP.R. China.
authors.forename: Kexin
authors.initials: K
authors.lastname: Lou
authors.affiliation: Department of Gynecology and Obstetrics, Xuzhou Central HospitalXuzhou, JiangsuP.R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Chen
authors.affiliation: Department of Ultrasound, Xuzhou Central HospitalXuzhou, JiangsuP.R. China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Gynecology and Obstetrics, Xuzhou Central HospitalXuzhou, JiangsuP.R. China.
authors.forename: Bei
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Central Laboratory, Xuzhou Central HospitalXuzhou, JiangsuP.R. China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Ultrasound, Xuzhou Central HospitalXuzhou, JiangsuP.R. China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Ultrasound, Xuzhou Central HospitalXuzhou, JiangsuP.R. China.
authors.forename: Haining
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Ultrasound, Xuzhou Central HospitalXuzhou, JiangsuP.R. China.
authors.forename: Dongwei
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Ultrasound, Xuzhou Central HospitalXuzhou, JiangsuP.R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D059447:Cell Cycle Checkpoints / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics; D000071161:Forkhead Box Protein O1 / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*
chemical_list: D000071161:Forkhead Box Protein O1; C555159:MIRN142 microRNA, human; D035683:MicroRNAs