title: Overexpression of MoSM1, encoding for an immunity-inducing protein from Magnaporthe oryzae, in rice confers broad-spectrum resistance against fungal and bacterial diseases.
abstract: Potential of MoSM1, encoding for a cerato-platanin protein from Magnaporthe oryzae, in improvement of rice disease resistance was examined. Transient expression of MoSM1 in rice leaves initiated hypersensitive response and upregulated expression of defense genes. When transiently expressed in tobacco leaves, MoSM1 targeted to plasma membrane. The MoSM1-overexpressing (MoSM1-OE) transgenic rice lines showed an improved resistance, as revealed by the reduced disease severity and decreased in planta pathogen growth, against 2 strains belonging to two different races of M. oryzae, causing blast disease, and against 2 strains of Xanthomonas oryzae pv. oryzae, causing bacterial leaf blight disease. However, no alteration in resistance to sheath blight disease was observed in MoSM1-OE lines. The MoSM1-OE plants contained elevated levels of salicylic acid (SA) and jasmonic acid (JA) and constitutively activated the expression of SA and JA signaling-related regulatory and defense genes. Furthermore, the MoSM1-OE plants had no effect on drought and salt stress tolerance and on grain yield. We conclude that MoSM1 confers a broad-spectrum resistance against different pathogens through modulating SA- and JA-mediated signaling pathways without any penalty on abiotic stress tolerance and grain yield, providing a promising potential for application of MoSM1 in improvement of disease resistance in crops.
authors.affiliation: National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P. R. China.
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Hong
authors.affiliation: National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P. R. China.
authors.forename: Yayun
authors.initials: Y
authors.lastname: Yang
authors.affiliation: National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P. R. China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P. R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.affiliation: National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P. R. China.
authors.forename: Dayong
authors.initials: D
authors.lastname: Li
authors.affiliation: National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P. R. China.
authors.forename: Fengming
authors.initials: F
authors.lastname: Song
mesh_terms: D003517:Cyclopentanes / Q000378:metabolism; D060467:Disease Resistance; D005656:Fungal Proteins / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D020082:Magnaporthe / Q000502:physiology*; D012275:Oryza / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D054883:Oxylipins / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D018515:Plant Leaves / Q000378:metabolism / Q000382:microbiology; D030821:Plants, Genetically Modified; D020156:Salicylic Acid / Q000378:metabolism; D014026:Nicotiana / Q000378:metabolism / Q000382:microbiology
chemical_list: D003517:Cyclopentanes; D005656:Fungal Proteins; D054883:Oxylipins; C011006:jasmonic acid; D020156:Salicylic Acid