title: Molecularly imprinted cryogel membranes for mitomycin C delivery.
abstract: In this study, cryogel-based implantable molecularly imprinted drug delivery systems were designed for the delivery of antineoplastic agent. Mitomycin C imprinted poly(2-hydroxyethyl methacrylate-N-methacryloyl-l-glutamic acid) cryogel membranes were produced by free-radical bulk polymerization under partially frozen conditions. The membranes were characterized by swelling tests, Fourier transform infrared spectroscopy, scanning electron microscopy, surface area measurements and in vitro hemocompatibility tests. In vitro delivery studies were carried out to examine the effects of cross-linker ratio and template content. Mitomycin C imprinted cryogel membranes have megaporous structure (10-100 μm in diameter). The cumulative release of mitomycin C was decreased with increasing cross-linking agent ratio and increased with the amount of template in the cryogel structure. The nature of transport mechanism of the mitomycin C from the membranes was non-Fickian.
authors.affiliation: a Bioengineering Division , Hacettepe University , Ankara , Turkey.
authors.forename: Pınar
authors.initials: P
authors.lastname: Öncel
authors.affiliation: b Biochemistry Division, Department of Chemistry , Hacettepe University , Ankara , Turkey.
authors.forename: Kemal
authors.initials: K
authors.lastname: Çetin
authors.affiliation: c Department of Chemistry , Aksaray University , Aksaray , Turkey.
authors.forename: Aykut Arif
authors.initials: AA
authors.lastname: Topçu
authors.affiliation: b Biochemistry Division, Department of Chemistry , Hacettepe University , Ankara , Turkey.
authors.forename: Handan
authors.initials: H
authors.lastname: Yavuz
authors.affiliation: b Biochemistry Division, Department of Chemistry , Hacettepe University , Ankara , Turkey.
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D003432:Cross-Linking Reagents / Q000737:chemistry; D059985:Cryogels / Q000737:chemistry*; D016503:Drug Delivery Systems; D065546:Drug Liberation; D006801:Humans; D008422:Materials Testing / Q000379:methods; D008567:Membranes, Artificial; D051379:Mice; D016685:Mitomycin / Q000737:chemistry* / Q000494:pharmacology*; D054802:Molecular Imprinting / Q000379:methods*; D058105:Polymerization; D016062:Porosity; D013499:Surface Properties
chemical_list: D000180:Acrylic Resins; D003432:Cross-Linking Reagents; D059985:Cryogels; D008567:Membranes, Artificial; D016685:Mitomycin
keywords: Cryogel membrane; mitomycin C delivery; molecular imprinting