title: Haloterrigena mahii sp. nov., an extremely halophilic archaeon from a solar saltern.
abstract: A pleomorphic, gas-vesicle-containing, halophilic archaeon, designated strain H13T, was isolated from the solar saltern of the Western Salt Co., Chula Vista, California, USA. Cells of strain H13T were non-motile, rod-shaped and 3-10 µm in length. The optimum growth conditions were 3.5-5.0 M NaCl, 45-55 °C, and pH range of 6.5-8.2. The major polar lipids were C20C20 and C20C25 derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and disulfated diglycosyl diether-1. The G+C content of he genome of strain H13T was calculated as 65.10 mol%. Phylogenetic analysis of 16S rRNA and rpoB' genes revealed that strain H13 was most closely related to Haloterrigena saccharevitans AB14T (16S rRNA gene sequence similarity: 99.51 %; rpoB' sequence similarity: 96.19 %) and Haloterrigena thermotolerans PR5T (99.11 %; 95.50 %). Strain H13T showed low genome relatedness values with Htg. saccharevitans AB14T and Htg. thermotolerans PR5T based on estimated average nucleotide identity (ANI; 92.59 and 91.68 %, respectively) and genome-to-genome distance analysis (GGDA; 47.90 and 45.00 %, respectively). Based on the phenotypic, chemotaxonomic and phylogenetic properties and the genome relatedness, it is evident that strain H13T represents a novel species of the genus Haloterrigena, for which the name Haloterrigena mahiisp. nov. is proposed. The type strain is H13T (=BCRC 910151T=NBRC 111885T).
authors.affiliation: Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan, ROC.
authors.forename: Jiun-Yan
authors.initials: JY
authors.lastname: Ding
authors.affiliation: Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan, ROC.
authors.forename: Sheng-Chung
authors.initials: SC
authors.lastname: Chen
authors.affiliation: Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan, ROC.|Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan, ROC.
authors.forename: Mei-Chin
authors.initials: MC
authors.lastname: Lai
authors.affiliation: Department of Medical Research, Taichung Veterans General Hospital, Taichung, Taiwan, ROC.
authors.forename: Tsai-Ling
authors.initials: TL
authors.lastname: Liao
mesh_terms: D001482:Base Composition; D002140:California; D019641:DNA, Archaeal / Q000235:genetics; D006216:Halobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D019641:DNA, Archaeal; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S