title: Upregulated effects of miR-7 in methicillin-resistantStaphylococcus aureus.
abstract: The aim of the study was to investigate the characteristic function of the upregulated effects of miR-7 in methicillin-resistantStaphylococcus aureus(MRSA). After separating the MRSA in clinic, the expression of miR-7 mRNA was tested by reverse transcription polymerase chain reaction. The overexpression, inhibition of miR-7, and control group were established by plasmidin vitro. Following transfection of the bacterial strain, the effect of β-lactam antibiotics in minimum inhibitory concentration (MIC) was observed using the microporous dilution method, and antibacterial effectsin vitrowere observed using the dynamic growth curve method. The expression of miR-7 in sensitive MRSA was upregulated distinctly, with significant difference (P<0.05). MIC and the number of bacteria in the miR-7 overexpression group significantly increased while the inhibition group decreased prominently, with significant difference (P<0.05). The control and null plasmid groups revealed no significant difference. In conclusion, miR-7 upregulated the antimicrobial activity of MRSA, and the intervention of its expression may become a possible antibacterial target.
authors.affiliation: Department of Clinical Laboratory, The Sixth People's Hospital of Jinan, Jinan, Shandong 250200, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Nursing, The Sixth People's Hospital of Jinan, Jinan, Shandong 250200, P.R. China.
authors.forename: Haiqing
authors.initials: H
authors.lastname: Li
authors.affiliation: Health Management Center, The Sixth People's Hospital of Jinan, Jinan, Shandong 250200, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Clinical Laboratory, The Sixth People's Hospital of Jinan, Jinan, Shandong 250200, P.R. China.
authors.forename: Qingyan
authors.initials: Q
authors.lastname: Li
authors.affiliation: Operation Room, The Sixth People's Hospital of Jinan, Jinan, Shandong 250200, P.R. China.
authors.forename: Yufang
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Department of Clinical Laboratory, Jinan Stomatological Hospital, Jinan, Shandong 250001, P.R. China.
authors.forename: Guiqing
authors.initials: G
authors.lastname: Fang
keywords: dynamic growth curve method; methicillin-resistant Staphylococcus aureus; miR-7; microporous dilution method; upregulation