title: Identification of a novel recombinant virulent avian infectious bronchitis virus.
abstract: The emergence of new infectious bronchitis virus (IBV) variants is often disastrous in the poultry industry. In this study, an IBV, CK/CH/2010/JT-1, was isolated from an H120- and 4/91-IBV-vaccinated flock in China. Antisera against vaccine strains H120 and 4/91 could not provide effective protection against CK/CH/2010/JT-1 in virus neutralization assays. CK/CH/2010/JT-1 could cause 43.75% mortality with respiratory and severe renal lesions in inoculated chickens. Phylogenetic analysis of the S1 gene showed that CK/CH/2010/JT-1 and 31 other isolates could be grouped as a new genotypic cluster. Recombination analysis revealed that three recombination events could be found in the genome of CK/CH/2010/JT-1 at positions 24709-365, 17160-19811 and 21136-21770. Whole-genome sequence analysis showed that CK/CH/2010/JT-1 originated from multiple template switches among QX-like, CK/CH/LSC/99I-, tl/CH/LDT3/03- and 4/91-type IBVs. All of these data demonstrated that CK/CH/2010/JT-1 is a new recombinant genotype IBV with high virulence. Our findings suggest that the surveillance of new genotype strains of IBV is very important for developing more effective anti-IBV strategies.
authors.affiliation: Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, Jiangsu 225009, PR China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China. Electronic address: 290368247@qq.com.
authors.forename: Haisheng
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, Jiangsu 225009, PR China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China. Electronic address: 317359898@qq.com.
authors.forename: Meihong
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, Jiangsu 225009, PR China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China. Electronic address: 1297056040@qq.com.
authors.forename: Xue
authors.initials: X
authors.lastname: Tian
authors.affiliation: Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, Jiangsu 225009, PR China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China; Jiangsu Key Lab of Zoonosis, Yangzhou 225009, PR China. Electronic address: hxshao@yzu.edu.cn.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Shao
authors.affiliation: Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, Jiangsu 225009, PR China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China; Jiangsu Key Lab of Zoonosis, Yangzhou 225009, PR China. Electronic address: qiankun@yzu.edu.cn.
authors.forename: Kun
authors.initials: K
authors.lastname: Qian
authors.affiliation: Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, Jiangsu 225009, PR China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China; Jiangsu Key Lab of Zoonosis, Yangzhou 225009, PR China. Electronic address: jqye@yzu.edu.cn.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Ye
authors.affiliation: Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, Jiangsu 225009, PR China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China; Jiangsu Key Lab of Zoonosis, Yangzhou 225009, PR China. Electronic address: aijian@yzu.edu.cn.
authors.forename: Aijian
authors.initials: A
authors.lastname: Qin
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000235:genetics; D018352:Coronavirus Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D016679:Genome, Viral / Q000235:genetics; D005838:Genotype; D007106:Immune Sera / Q000276:immunology; D001351:Infectious bronchitis virus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D011200:Poultry; D011201:Poultry Diseases / Q000473:pathology / Q000821:virology*; D014774:Virulence / Q000235:genetics
chemical_list: D036022:Capsid Proteins; D007106:Immune Sera
keywords: Genome; Infectious bronchitis virus; Recombinant; Virulence