title: Replication kinetics of neuropathogenic and non-neuropathogenic equine herpesvirus type 1 (EHV-1) strains in primary murine neurons and ED cell line.
abstract: Equine herpesvirus type 1 (EHV-1) causes respiratory infections, abortion and neurological disorders in horses. Molecular epidemiology studies have demonstrated that a single-point mutation in DNA polymerase gene, resulting in an amino acid variation (N752/D752), is significantly associated with the neuropathogenic potential of EHV-1 strains. The aim of the study was to elucidate if there are any differences between neuropathogenic (EHV-1 26) and non-neuropathogenic (Jan-E and Rac-H) EHV-1 strains in their ability to infect neuronal cells. For the tested EHV-1 strains, cytopathic effect (CPE) was manifested by changed morphology of cells, destruction of actin cytoskeleton and nuclei degeneration, which led to focal degeneration. Moreover, EHV-1 26 strain caused fusion of the infected cells to form syncytia in culture. Real-time PCR analysis demonstrated that both neuropathogenic and non-neuropathogenic EHV-1 strains replicated in neurons and ED cells (equine dermal cell line) at a similar level. We can assume that a point mutation in the EHV-1 polymerase does not affect viral replication in this cell type.
authors.forename: J
authors.initials: J
authors.lastname: Cymerys
authors.forename: A
authors.initials: A
authors.lastname: Słońska
authors.forename: J
authors.initials: J
authors.lastname: Brzezicka
authors.forename: A
authors.initials: A
authors.lastname: Tucholska
authors.forename: A
authors.initials: A
authors.lastname: Chmielewska
authors.forename: J
authors.initials: J
authors.lastname: Rola
authors.forename: P
authors.initials: P
authors.lastname: Malik
authors.forename: M W
authors.initials: MW
authors.lastname: Bańbura
mesh_terms: D000818:Animals; D002460:Cell Line; D005347:Fibroblasts / Q000821:virology*; D004861:Herpesvirus 1, Equid / Q000502:physiology*; D006736:Horses; D051379:Mice; D009474:Neurons / Q000821:virology*; D013997:Time Factors; D014779:Virus Replication / Q000502:physiology*