title: Separation of Vitamin E on a 100-Å Phenogel Column.
abstract: The effect of π-electrons and hydroxyl group on the separations of vitamin E on a swelling-controlled polystyrene-divinylbenzene (Phenogel) column using toluene/isooctane as the mobile phase was investigated. The effect of the π-electrons was demonstrated in the baseline separation of α-tocopherol and α-tocotrienol on a 100-Å Phenogel column. In addition, baseline separation of α-, (β- + γ-)- and δ-tocopherol could be achieved on this column. The separation mechanism of these isomers are due to the difference in the interactions between the hydroxyl group on the chromanol ring of each tocopherol and the gel matrix caused by the steric hindrance of methyl group(s). It was concluded that solutes of the same molecular size but different in the polar groups could be separated on a high performance size-exclusion chromatography by controlling the swelling of the gel matrix via modification of the mobile phase.
authors.affiliation: Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, 49 Soi Thian Thale 25, Bang Khun Thian Chai Thale Road, Tha Kham, Bang Khun Thian, Bangkok 10150, Thailand.
authors.forename: Salisa
authors.initials: S
authors.lastname: Chumsantea
authors.affiliation: Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, 49 Soi Thian Thale 25, Bang Khun Thian Chai Thale Road, Tha Kham, Bang Khun Thian, Bangkok 10150, Thailand kornkanok.ary@kmutt.ac.th.
authors.forename: Kornkanok
authors.initials: K
authors.lastname: Aryusuk
authors.affiliation: Department of Nutrition, Faculty of Public Health, Mahidol University, Ratchathewi, Bangkok 10400, Thailand.
authors.forename: Supathra
authors.initials: S
authors.lastname: Lilitchan
authors.affiliation: Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, 49 Soi Thian Thale 25, Bang Khun Thian Chai Thale Road, Tha Kham, Bang Khun Thian, Bangkok 10150, Thailand.
authors.forename: Narumon
authors.initials: N
authors.lastname: Jeyashoke
authors.affiliation: Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, 49 Soi Thian Thale 25, Bang Khun Thian Chai Thale Road, Tha Kham, Bang Khun Thian, Bangkok 10150, Thailand.
authors.forename: Kanit
authors.initials: K
authors.lastname: Krisnangkura
mesh_terms: D002850:Chromatography, Gel / Q000295:instrumentation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D011137:Polystyrenes / Q000737:chemistry*; D015203:Reproducibility of Results; D014810:Vitamin E / Q000032:analysis* / Q000302:isolation & purification*
chemical_list: D011137:Polystyrenes; C432006:divinylbenzene-polystyrene copolymer; D014810:Vitamin E