title: LSP1, a responsive protein from Meyerozyma guilliermondii, elicits defence response and improves glycyrrhizic acid biosynthesis in Glycyrrhiza uralensis Fisch adventitious roots.
abstract: This research explored the effects of protein and polysaccharide in Meyerozyma guilliermondii on active compounds in Glycyrrhiza uralensis Fisch adventitious roots. In this study, a responsive protein LSP1 was purified from the Meyerozyma guilliermondii since the excellent induction. The contents of total flavonoids (3.46 mg · g-1), glycyrrhizic acid (0.41 mg · g-1), glycyrrhetinic acid (0.41 mg · g-1), and polysaccharide (94.49 mg · g-1) in adventitious root peaked at LSP1 group, which were 1.6, 3.4, 2.4, 2.0-fold that of control, respectively. Besides, the responsive protein LSP1 significantly activated the defense signaling, mitogen-activated protein kinases and extremely up-regulated the expression of defense-related genes and functional genes involved in glycyrrhizic acid biosynthesis.
authors.affiliation: Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China.|Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology, College of Biotechnology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, China.
authors.forename: Jianli
authors.initials: J
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China.|Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China.|Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin, China.
authors.forename: Jinxin
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology, College of Biotechnology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, China.
authors.forename: Shujie
authors.initials: S
authors.lastname: Liu
authors.affiliation: Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China.|Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin, China.
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Gao
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005656:Fungal Proteins / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D029882:Glycyrrhiza uralensis / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D019695:Glycyrrhizic Acid / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism* / Q000382:microbiology*; D004718:Saccharomycetales / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D021241:Spectrometry, Mass, Electrospray Ionization; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D005656:Fungal Proteins; D019695:Glycyrrhizic Acid; D020928:Mitogen-Activated Protein Kinases
keywords: Glycyrrhiza uralensis Fisch; Meyerozyma guilliermondii; adventitious root; defense gene; functional gene; signaling molecule