title: Studying hematopoiesis using single-cell technologies.
abstract: Hematopoiesis is probably the best-understood stem cell differentiation system; hematopoietic stem cell (HSC) transplantation represents the most widely used regenerative therapy. The classical view of lineage hierarchy in hematopoiesis is built on cell type definition system by a group of cell surface markers. However, the traditional model is facing increasing challenges, as many classical cell types are proved to be heterogeneous. Recently, the developments of new technologies allow genome, transcriptome, proteome, and epigenome analysis at the single-cell level. For the first time, we can study hematopoietic system at single-cell resolution on a multi-omic scale. Here, we review recent technical advances in single-cell analysis technology, as well as their current applications. We will also discuss the impact of single-cell technologies on both basic research and clinical application in hematology.
authors.affiliation: Center for Stem Cell and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou, 310058, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Ye
authors.affiliation: Center for Stem Cell and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou, 310058, China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Huang
authors.affiliation: Center for Stem Cell and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou, 310058, China. ggj@zju.edu.cn.
authors.forename: Guoji
authors.initials: G
authors.lastname: Guo
mesh_terms: D000818:Animals; D023281:Genomics; D006410:Hematopoiesis; D006801:Humans; D040901:Proteomics; D059010:Single-Cell Analysis / Q000379:methods*
keywords: Hematopoietic stem cell; Lineage hierarchy; Regulatory network; Single-cell analysis