title: Effects of treatment with sucrose in drinking water on liver histology, lipogenesis and lipogenic gene expression in rats fed high-fiber diet.
abstract: We studied the influence of sucrose in drinking water on liver histology, fatty acid profile and lipogenic genes expression in rats maintained on high-fiber. The experimental groups were: control group (water) and sucrose group (sucrose solution in drinking water, 30% w/v). Liver histology of sucrose treated rats revealed steatosis and increased number of αSMA immunoreactive cells without the signs of fibrosis. Sucrose treatment increased de novo lipogenesis, lipid peroxidation and MUFA content and decreased PUFA content, C18:2n6 and C20:4n6 content in total phospholipids and phosphatidylethanolamine and C18:2n6 content in cardiolipin. RT-qPCR revealed increase in Δ-9-desaturase and SREBP1c gene expression and decrease in the Δ-5-desaturase and elongase 5 expression. Treatment with sucrose extensively changes fatty acid composition of hepatic lipid and phospholipid classes including cardiolipin, increases oxidative stress and causes pathological changes in liver in rats maintained on high-fiber diet.
authors.affiliation: Department of Animal Nutrition and Dietetics, University of Zagreb, Faculty of Veterinary Medicine, Heinzelova 55, 10000 Zagreb, Croatia. Electronic address: tomislav.masek@vef.hr.
authors.forename: Tomislav
authors.initials: T
authors.lastname: Mašek
authors.affiliation: Department of Anatomy, Histology and Embryology, University of Split, School of Medicine, Split, Croatia.
authors.forename: Natalija
authors.initials: N
authors.lastname: Filipović
authors.affiliation: Department of Anatomy, Histology and Embryology, University of Split, School of Medicine, Split, Croatia.
authors.forename: Ana
authors.initials: A
authors.lastname: Vuica
authors.affiliation: Department of Animal Husbandry, University of Zagreb Faculty, of Veterinary Medicine, Zagreb, Croatia.
authors.forename: Kristina
authors.initials: K
authors.lastname: Starčević
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D000090943:Delta-5 Fatty Acid Desaturase; D059305:Diet, High-Fat / Q000009:adverse effects*; D060766:Drinking Water / Q000737:chemistry*; D044943:Fatty Acid Desaturases / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005786:Gene Expression Regulation / Q000187:drug effects; D053263:Gene Regulatory Networks / Q000187:drug effects*; D015227:Lipid Peroxidation; D050155:Lipogenesis / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000473:pathology*; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D013230:Stearoyl-CoA Desaturase / Q000235:genetics; D051779:Sterol Regulatory Element Binding Proteins / Q000235:genetics; D013395:Sucrose / Q000008:administration & dosage*
chemical_list: D000199:Actins; D000090943:Delta-5 Fatty Acid Desaturase; D060766:Drinking Water; D044943:Fatty Acid Desaturases; D005227:Fatty Acids; D013230:Stearoyl-CoA Desaturase; D051779:Sterol Regulatory Element Binding Proteins; D013395:Sucrose; C470194:Srebf2 protein, rat; C498862:Acta2 protein, rat
keywords: Cardiolipin; Lipogenesis; Phosphatidylethanolamine; Sucrose