title: MiR-320a effectively suppresses lung adenocarcinoma cell proliferation and metastasis by regulating STAT3 signals.
abstract: MicroRNAs play important roles in tumorigenesis of various types of cancers. MiR-320a can inhibits cell proliferation of some cancers, but the biologic roles of miR-320a in lung cancer need to be further studied. Here, we investigated the roles of miR-320a in suppressing the proliferation of lung adenocarcinoma cells. MiR-320a treatment was found to effectively suppress LTEP-a-2 and A549 cell proliferation, and induce more apoptotic cells with irradiation treatment compared with control treatment. Our results also showed that miR-320a, as a novel miRNA, directly regulated signal transducer and activator of transcription 3 (STAT3) and its signals, such as Bcl-2, Bax, and Caspase 3. The siRNA-inhibited STAT3 levels further proved its roles in regulating STAT3 signals. Moreover, miR-320a treatment effectively suppressed cancer cell growth in mice xenografts compared with controls, and significantly inhibited cell migration in vitro and in vivo. Our findings collectively demonstrated that miR-320a, by directly regulating STAT3 signals, not only suppressed cell proliferation and metastasis, but also enhanced irradiation-induced apoptosis of adenocarcinomia cells.
authors.affiliation: a Key Laboratory of Tumor Molecular Biology in Binzhou Medical University, Department of Biochemistry and Molecular Biology , Binzhou Medical University , YanTai , Shandong , P.R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Lv
authors.affiliation: a Key Laboratory of Tumor Molecular Biology in Binzhou Medical University, Department of Biochemistry and Molecular Biology , Binzhou Medical University , YanTai , Shandong , P.R. China.
authors.forename: Jin-Xia
authors.initials: JX
authors.lastname: Hu
authors.affiliation: a Key Laboratory of Tumor Molecular Biology in Binzhou Medical University, Department of Biochemistry and Molecular Biology , Binzhou Medical University , YanTai , Shandong , P.R. China.
authors.forename: You-Jie
authors.initials: YJ
authors.lastname: Li
authors.affiliation: b YanTaiShan Hospital , YanTai , Shandong , P.R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Xie
authors.affiliation: a Key Laboratory of Tumor Molecular Biology in Binzhou Medical University, Department of Biochemistry and Molecular Biology , Binzhou Medical University , YanTai , Shandong , P.R. China.
authors.forename: Dan Dan
authors.initials: DD
authors.lastname: Song
authors.affiliation: a Key Laboratory of Tumor Molecular Biology in Binzhou Medical University, Department of Biochemistry and Molecular Biology , Binzhou Medical University , YanTai , Shandong , P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: a Key Laboratory of Tumor Molecular Biology in Binzhou Medical University, Department of Biochemistry and Molecular Biology , Binzhou Medical University , YanTai , Shandong , P.R. China.
authors.forename: Yun-Fei
authors.initials: YF
authors.lastname: Yan
authors.affiliation: c Department of Radiation Oncology , Shandong Cancer Hospital, Shandong Academy of Medical Sciences , Jinan , P.R. China.
authors.forename: Bao-Sheng
authors.initials: BS
authors.lastname: Li
authors.affiliation: a Key Laboratory of Tumor Molecular Biology in Binzhou Medical University, Department of Biochemistry and Molecular Biology , Binzhou Medical University , YanTai , Shandong , P.R. China.
authors.forename: Ping-Yu
authors.initials: PY
authors.lastname: Wang
authors.affiliation: a Key Laboratory of Tumor Molecular Biology in Binzhou Medical University, Department of Biochemistry and Molecular Biology , Binzhou Medical University , YanTai , Shandong , P.R. China.
authors.forename: Shu-Yang
authors.initials: SY
authors.lastname: Xie
mesh_terms: D000072283:A549 Cells; D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology / Q000532:radiotherapy / Q000628:therapy*; D000077192:Adenocarcinoma of Lung; D000818:Animals; D049109:Cell Proliferation / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology / Q000532:radiotherapy / Q000628:therapy*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000008:administration & dosage* / Q000235:genetics*; D009362:Neoplasm Metastasis; D050796:STAT3 Transcription Factor / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014162:Transfection; D023041:Xenograft Model Antitumor Assays
chemical_list: C572916:MIRN320 microRNA, human; D035683:MicroRNAs; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human
keywords: Cell proliferation; NSCLC; STAT3; metastasis; miR-320a