title: An ultrasensitive aptasensor for Ochratoxin A using hexagonal core/shell upconversion nanoparticles as luminophores.
abstract: We developed an ultrasensitive luminescence resonance energy transfer (LRET) aptasensor for Ochratoxin A (OTA) detection, using core/shell upconversion nanoparticles (CS-UCNPs) as luminophores. The OTA aptamer was tagged to CS-UCNPs as energy donor and graphene oxide (GO) acted as energy acceptor. The π-π stacking interaction between the aptamer and GO brought CS-UCNPs and GO in close proximity hence initiated the LRET process resulting in quenching of CS-UCNPs luminescence. A linear calibration was obtained between the luminescence intensity and the logarithm of OTA concentration in the range from 0.001ngmL-1to 250ngmL-1, with a detection limit of 0.001ngmL-1. The aptasensor showed good specificity towards OTA in beer samples. The ultrahigh sensitivity and pronounced robustness in beer sample matrix suggested promising prospect of the aptasensor inpractical applications.
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Taicang Entry-Exit Inspection and Quarantine Bureau, Suzhou 215400, China.
authors.forename: Shaoliang
authors.initials: S
authors.lastname: Dai
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Shijia
authors.initials: S
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Nuo
authors.initials: N
authors.lastname: Duan
authors.affiliation: Taicang Entry-Exit Inspection and Quarantine Bureau, Suzhou 215400, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.affiliation: Taicang Entry-Exit Inspection and Quarantine Bureau, Suzhou 215400, China.
authors.forename: Zhigao
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. Electronic address: wangzp@jiangnan.edu.cn.
authors.forename: Zhouping
authors.initials: Z
authors.lastname: Wang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001515:Beer / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D004871:Erbium / Q000737:chemistry; D005459:Fluorides / Q000737:chemistry; D057230:Limit of Detection; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009793:Ochratoxins / Q000032:analysis*; D015019:Yttrium / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D049408:Luminescent Agents; D009793:Ochratoxins; C525362:sodium yttriumtetrafluoride; C025589:ochratoxin A; D015019:Yttrium; D004871:Erbium; D005459:Fluorides
keywords: Graphene oxide; Luminescence resonance energy transfer; Ochratoxin A; Upconversion nanoparticles