title: Expression Profiles Analysis and Functional Characterization of MicroRNA-660 in Skeletal Muscle Differentiation.
abstract: MicroRNA are a series of small non-coding RNAs that have emerged as critical regulators of skeletal muscle development. Here, we concentrated on the function of miR-660 during bovine skeletal myogenesis from our previous high-throughput sequencing results, then analyzed its expression profiles and characterized related functional roles. Overexpression of miR-660 significantly attenuated myogenic differentiation of C2C12 cells, whereas miR-660 inhibition enhanced C2C12 differentiation. Dual-Luciferase Reporter Assay went for demonstrating that miR-660 directly targeted the 3'-UTR of Rho guanine nucleotide exchange factor 12 (ARHGEF-12). Furthermore, we found an inverse relationship between the expression of miR-660 and ARHGEF12 in both gain- and loss-of-function studies: overexpression of miR-660 declined the mRNA and protein expressions of ARHGEF12 in C2C12 cells differentiation; however, knockdown of miR-660 had completely opposite results. Taken together, these results offered a new perspective for miR-660 in skeletal muscle differentiation. J. Cell. Biochem. 118: 2387-2394, 2017. © 2017 Wiley Periodicals, Inc.
authors.affiliation: Institute of Cellular and Molecular Biology, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, P.R. China.
authors.forename: Binglin
authors.initials: B
authors.lastname: Yue
authors.affiliation: Institute of Cellular and Molecular Biology, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, P.R. China.
authors.forename: Jiyao
authors.initials: J
authors.lastname: Wu
authors.affiliation: Institute of Cellular and Molecular Biology, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, P.R. China.
authors.forename: Yanhuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Cellular and Molecular Biology, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, P.R. China.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Institute of Cellular and Molecular Biology, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, P.R. China.
authors.forename: Xingtang
authors.initials: X
authors.lastname: Fang
authors.affiliation: Institute of Cellular and Molecular Biology, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D024510:Muscle Development / Q000235:genetics / Q000502:physiology*; D018482:Muscle, Skeletal / Q000166:cytology* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D064067:Rho Guanine Nucleotide Exchange Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C577223:Arhgef12 protein, mouse; C000601115:MIRN-660 microRNA, mouse; D035683:MicroRNAs; D012333:RNA, Messenger; D064067:Rho Guanine Nucleotide Exchange Factors
keywords: ARHGEF12; DIFFERENTIATION; SKELETAL MUSCLE; miR-660