title: Bidirectional communication between sensory neurons and osteoblasts in an in vitro coculture system.
abstract: Recent studies have revealed that the sensory nervous system is involved in bone metabolism. However, the mechanism of communication between neurons and osteoblasts is yet to be elucidated. In this study, we investigated the signaling pathways between sensory neurons of the dorsal root ganglion (DRG) and the osteoblast-like MC3T3-E1 cells using an in vitro coculture system. Our findings indicate that signal transduction from DRG-derived neurons to MC3T3-E1 cells is suppressed by antagonists of the AMPA receptor and the NK1receptor. Conversely, signal transduction from MC3T3-E1 cells to DRG-derived neurons is suppressed by a P2X7receptor antagonist. Our results suggest that these cells communicate with each other by exocytosis of glutamate, substance P in the efferent signal, and ATP in the afferent signal.
authors.affiliation: Laboratory of Neuropharmacology, School of Pharmacy, Aichi-Gakuin University, Chikusa-ku, Nagoya, Japan.|Department of Pharmacology, School of Dentistry, Aichi-Gakuin University, Chikusa-ku, Nagoya, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kodama
authors.affiliation: Laboratory of Medicinal Resources, School of Pharmacy, Aichi-Gakuin University, Chikusa-ku, Nagoya, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Hirai
authors.affiliation: Department of Pharmacology, School of Dentistry, Aichi-Gakuin University, Chikusa-ku, Nagoya, Japan.
authors.forename: Hisataka
authors.initials: H
authors.lastname: Kondo
authors.affiliation: Department of Pharmacology, School of Dentistry, Aichi-Gakuin University, Chikusa-ku, Nagoya, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Hamamura
authors.affiliation: Department of Pharmacology, School of Dentistry, Aichi-Gakuin University, Chikusa-ku, Nagoya, Japan.
authors.forename: Akifumi
authors.initials: A
authors.lastname: Togari
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002450:Cell Communication / Q000187:drug effects; D002460:Cell Line; D018920:Coculture Techniques / Q000379:methods*; D005089:Exocytosis / Q000187:drug effects; D005727:Ganglia, Spinal / Q000166:cytology; D018698:Glutamic Acid / Q000378:metabolism; D006801:Humans; D008807:Mice, Inbred BALB C; D018377:Neurotransmitter Agents / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013373:Substance P / Q000378:metabolism
chemical_list: D018377:Neurotransmitter Agents; D013373:Substance P; D018698:Glutamic Acid; D000255:Adenosine Triphosphate; D001920:Bradykinin; D002118:Calcium
keywords: intercellular communication; osteoblast; sensory nervous system