title: Endophytic fungi from mangrove inhibit lung cancer cell growth and angiogenesis in vitro.
abstract: The secondary metabolites of mangrove-derived endophytic fungi contain multiple substances with novel structures and biological activities. In the present study, three types of mangrove plants, namely Kandelia candel, Rhizophora stylosa and Rhizophoraceae from Zhanjiang region including the leaves, roots and stems were collected, and endophytic fungi were isolated, purified and identified from these mangrove plants. MTT assay was used to observe the effects of the isolated endophytic fungi on the growth of A549 and NCI-H460 lung cancer cells. The effect of the endophytic fungi on lung cancer angiogenesis in vitro induced by the HPV-16 E7 oncoprotein was observed. Our results showed that 28 strains of endophytic fungi were isolated, purified and identified from the three types of mangrove plants. Ten strains of endophytic fungi significantly suppressed the growth of A549 and NCI-H460 cells. The average inhibitory rates in the A549 cells were 64.4, 59.5, 81.9, 43.9, 58.3, 56.2, 48.3, 42.4, 93.0 and 49.7%, respectively. The average inhibitory rates in the NCI-H460 cells were 41.2, 49.3, 82.7, 40.7, 53.9, 52.6, 56.8, 64.3, 91.0 and 45.6%, respectively. Particularly, three strains of endophytic fungi markedly inhibited HPV-16 E7 oncoprotein‑induced lung cancer angiogenesis in vitro. These findings contribute to the further screening of potential chemotherapeutic agents from mangrove-derived endophytic fungi.
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical University, Xiashan, Zhanjiang, Guangdong 524023, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical University, Xiashan, Zhanjiang, Guangdong 524023, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wu
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical University, Xiashan, Zhanjiang, Guangdong 524023, P.R. China.
authors.forename: Yuefan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Institute of Biochemistry and Molecular Biology, Guangdong Medical University, Xiashan, Zhanjiang, Guangdong 524023, P.R. China.
authors.forename: Wenzhang
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Institute of Biochemistry and Molecular Biology, Guangdong Medical University, Xiashan, Zhanjiang, Guangdong 524023, P.R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Hu
authors.affiliation: Institute of Biochemistry and Molecular Biology, Guangdong Medical University, Xiashan, Zhanjiang, Guangdong 524023, P.R. China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Feng
authors.affiliation: Institute of Biochemistry and Molecular Biology, Guangdong Medical University, Xiashan, Zhanjiang, Guangdong 524023, P.R. China.
authors.forename: Xiangyong
authors.initials: X
authors.lastname: Li
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical University, Xiashan, Zhanjiang, Guangdong 524023, P.R. China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Tang
mesh_terms: D049109:Cell Proliferation; D005455:Fluorescent Antibody Technique; D005658:Fungi / Q000472:pathogenicity*; D006801:Humans; D066298:In Vitro Techniques; D008175:Lung Neoplasms / Q000098:blood supply / Q000473:pathology / Q000517:prevention & control*; D009389:Neovascularization, Pathologic / Q000517:prevention & control*; D018515:Plant Leaves / Q000254:growth & development / Q000382:microbiology; D016133:Polymerase Chain Reaction; D043982:Rhizophoraceae / Q000254:growth & development / Q000382:microbiology*; D014407:Tumor Cells, Cultured