title: Production and characterization of medium-chain-length polyhydroxyalkanoate copolymer from Arctic psychrotrophic bacterium Pseudomonas sp. PAMC 28620.
abstract: Arctic psychrotrophic bacterium Pseudomonas sp. PAMC 28620 was found to produce a distinctive medium-chain-length polyhydroxyalkanoate (MCL-PHA) copolymer when grown on structurally unrelated carbon sources including glycerol. The maximum MCL-PHA copolymer yield was obtained about 52.18±4.12% from 7.95±0.66g/L of biomass at 144h of fermentation when 3% glycerol was used as sole carbon and energy source during the laboratory-scale bioreactor process. Characterization of the copolymer was carried out using fourier transform infrared spectroscopy (FTIR), gas chromatography-mass spectrometry (GC-MS), proton (1H) and carbon (13C) nuclear magnetic resonance spectroscopy (NMR), gel permeation chromatography (GPC), differential scanning calorimeter (DSC) and thermo-gravimetric analysis (TGA). The copolymer produced by Pseudomonas sp. PAMC 28620 consisting of four PHA monomers and identified as 3-hydroxyoctanoate (3HO), 3-hydroxydecanoate (3HD), 3-hydroxydodecanoate (3HDD) and 3-hydroxytetradecanoate (3HTD). An average molecular weight of the copolymer was found approximately 30.244kDa with polydispersity index (PDI) value of 2.05. Thermal analysis showed the produced MCL-PHA copolymer to be low-crystalline (43.73%) polymer with great thermal stability, having the thermal decomposition temperature of 230°C-280°C, endothermic melting temperature (Tm) of 172.84°C, glass transition (Tg) temperature of 3.99°C, and apparent melting enthalpy fusion (ΔHm) about 63.85Jg-1.
authors.affiliation: Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, South Korea.
authors.forename: Ganesan
authors.initials: G
authors.lastname: Sathiyanarayanan
authors.affiliation: Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, South Korea.
authors.forename: Shashi Kant
authors.initials: SK
authors.lastname: Bhatia
authors.affiliation: Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, South Korea.
authors.forename: Hun-Suk
authors.initials: HS
authors.lastname: Song
authors.affiliation: Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, South Korea.
authors.forename: Jong-Min
authors.initials: JM
authors.lastname: Jeon
authors.affiliation: Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, South Korea.
authors.forename: Junyoung
authors.initials: J
authors.lastname: Kim
authors.affiliation: Division of Life Sciences, Korea Polar Research Institute, 26 Songdomirae-ro, Yeonsu-gu, Incheon 21990, South Korea.
authors.forename: Yoo Kyung
authors.initials: YK
authors.lastname: Lee
authors.affiliation: Department of Chemical Engineering, Soongsil University, 511 Sangdo-dong, Seoul 06978, South Korea.
authors.forename: Yun-Gon
authors.initials: YG
authors.lastname: Kim
authors.affiliation: Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, South Korea; Institute for Ubiquitous Information Technology and Applications, Konkuk University, Seoul 05029, South Korea. Electronic address: seokor@konkuk.ac.kr.
authors.forename: Yung-Hun
authors.initials: YH
authors.lastname: Yang
mesh_terms: D001110:Arctic Regions; D002244:Carbon / Q000378:metabolism; D008970:Molecular Weight; D009584:Nitrogen / Q000378:metabolism; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis* / Q000737:chemistry*; D011549:Pseudomonas / Q000378:metabolism*; D013696:Temperature
chemical_list: D054813:Polyhydroxyalkanoates; D002244:Carbon; D009584:Nitrogen
keywords: Arctic Pseudomonas; Copolymer; Fermentation; Glycerol; Polyhydroxyalkanoates