title: Effects of Release-Active Antibodies to CD4 Receptor on the Level of lck-Kinase in Cultured Mononuclear Cells from Human Peripheral Blood.
abstract: For evaluation of effects of release-active antibodies to CD4 on cultured lymphocytes from human peripheral blood, we measured intracellular content of lck-kinase cell-based ELISA. In cells treated with release-active antibodies to CD4, the content of intracellular lck-kinase significantly (p<0.01) decreased in comparison with the control (purified water processed in a similar way). Phytohemagglutinin had no effect on the concentration of lck-kinase in cells. The decrease in the content of CD4-associated lck protein suggests that the preparation enhanced intracellular coupling of lck-kinase with T-cell receptor and potentiated T-cell immune response.
authors.affiliation: 1Laboratory of Physiologically Active Substances, Department of Molecular and Cellular Pathophysiology, Research Institute of General Pathology and Pathophysiology, Moscow, Russia. agemelyanova@gmail.com.
authors.forename: A G
authors.initials: AG
authors.lastname: Emel'yanova
authors.affiliation: Research and Production Company Materia Medica Holding, Moscow, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Grechenko
authors.affiliation: 1Laboratory of Physiologically Active Substances, Department of Molecular and Cellular Pathophysiology, Research Institute of General Pathology and Pathophysiology, Moscow, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Petrova
authors.affiliation: State Research Centre Institute of Immunology, Federal Medical-Biological Agency of the Russian Federation, Moscow, Russia.
authors.forename: I P
authors.initials: IP
authors.lastname: Shilovskii
authors.affiliation: Research and Production Company Materia Medica Holding, Moscow, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Gorbunov
authors.affiliation: Research and Production Company Materia Medica Holding, Moscow, Russia.
authors.forename: S A
authors.initials: SA
authors.lastname: Tarasov
authors.affiliation: State Research Centre Institute of Immunology, Federal Medical-Biological Agency of the Russian Federation, Moscow, Russia.
authors.forename: M R
authors.initials: MR
authors.lastname: Khaitov
authors.affiliation: 1Laboratory of Physiologically Active Substances, Department of Molecular and Cellular Pathophysiology, Research Institute of General Pathology and Pathophysiology, Moscow, Russia.
authors.forename: S G
authors.initials: SG
authors.lastname: Morozov
authors.affiliation: 1Laboratory of Physiologically Active Substances, Department of Molecular and Cellular Pathophysiology, Research Institute of General Pathology and Pathophysiology, Moscow, Russia.
authors.forename: O I
authors.initials: OI
authors.lastname: Epshtein
mesh_terms: D000328:Adult; D000906:Antibodies / Q000494:pharmacology*; D015704:CD4 Antigens / Q000235:genetics* / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / Q000235:genetics* / Q000276:immunology; D008297:Male; D010835:Phytohemagglutinins / Q000494:pharmacology; D061251:Primary Cell Culture
chemical_list: D000906:Antibodies; D015704:CD4 Antigens; D010835:Phytohemagglutinins; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
keywords: CD4; Ergoferon; antibodies; lck-kinase; release-activity