title: Towards perylenequinonoid: Effective application to reversible fluorescent probe for monitoring hydrogen persulfide in solvents and living cells.
abstract: Hydrogen persulfide (H2S2), as a direct redox form of H2S, may have its own physiological processes in maintaining intracellular redox homeostasis. The research on hydrogen persulfide was rapidly growing. As it is the actual signalling molecules derived from hydrogen sulfide, reversible detection of hydrogen persulfide changes in cells is of great significance. To address this critical need, we present a reversible fluorescent switch-on H2S2probe, the tautomeric of 6, 7-dihydroxyperylene-1, 12-quinone (DHPQ), capable of tracking H2S2in solvents and living cells. The probe can be applied to quantified H2S2expressions ranging linearly from 2.0×10-6to 2.0×10-5M. The limit of detection was found to be 1.82×10-8M. In addition, the fluorescent alterations are remarkably specific for H2S2in the presence of other reactive sulfur species. These features are favorable for imaging application. Taking advantage of favorable cycle stability and admirable selectivity, the DHPQ dye can be used as a reversible fluorescent platform to construct novel H2S2probes. Since such reversible H2S2probes are rare, this work provide a potential approach for reversible probing fluctuation of hydrogen persulfide in biological systems.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, PR China.
authors.forename: Tiancheng
authors.initials: T
authors.lastname: Qiu
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, PR China.
authors.forename: Haipeng
authors.initials: H
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, PR China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, PR China.
authors.forename: Chuanglin
authors.initials: C
authors.lastname: Jiang
authors.affiliation: College of Environmental and Engineering, Nanchang Hangkong University, Nanchang 330063, PR China. Electronic address: sllou@hnu.edu.cn.
authors.forename: Shenglian
authors.initials: S
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, PR China. Electronic address: zbzeng@hnu.edu.cn.
authors.forename: Zebing
authors.initials: Z
authors.lastname: Zeng
mesh_terms: D002470:Cell Survival; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis* / Q000737:chemistry*; D061848:Optical Imaging; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry*; D011809:Quinones / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D011809:Quinones; D012997:Solvents; D010569:Perylene; D006862:Hydrogen Sulfide
keywords: Bioimaging; Excited-state intramolecular hydrogen atom transfer; Hydrogen persulfide detection; Perylenequinonoid dye; Reversible fluorescent probe