title: 1H,13C and15N backbone and side-chain resonance assignments of the ZnF-UBP domain of USP20/VDU2.
abstract: Deubiquitinase USP20/VDU2 has been identified as a regulator of multiple proteins including hypoxia-inducible factor (HIF)-1α, β2-adrenergic receptor, and tumor necrosis factor receptor associated factor 6 etc. It contains four structural domains, including an N-terminal zinc-finger ubiquitin binding domain (ZnF-UBP) that potentially helps USP20 to recruit its ubiquitin substrates. Here we report the1H,13C and15N backbone and side-chain resonance assignments of the ZnF-UBP domain of USP20/VDU2. The BMRB accession number is 26901. The secondary structural elements predicted from the NMR data reveal a global fold consisting of three α-helices and four β-strands. The complete assignments can be used to explore the protein dynamics of the USP20 ZnF-UBP and its interactions with monoubiquitin and ubiquitin chains.
authors.affiliation: CAS Key Laboratory of Receptor Research, Department of Analytical Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, People's Republic of China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: CAS Key Laboratory of Receptor Research, Department of Analytical Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, People's Republic of China. wenyi1984112@126.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wen
authors.affiliation: CAS Key Laboratory of Receptor Research, Department of Analytical Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, People's Republic of China. nxzhang@simm.ac.cn.
authors.forename: Naixia
authors.initials: N
authors.lastname: Zhang
mesh_terms: D019906:Nuclear Magnetic Resonance, Biomolecular; D000072417:Protein Domains; D025801:Ubiquitin / Q000378:metabolism*; D043222:Ubiquitin Thiolesterase / Q000737:chemistry* / Q000378:metabolism*; D016335:Zinc Fingers
chemical_list: C459591:USP20 protein, human; D025801:Ubiquitin; D043222:Ubiquitin Thiolesterase
keywords: Deubiquitinase; NMR; USP20; ZnF-UBP domain