title: Pedobacter lusitanus sp. nov., isolated from sludge of a deactivated uranium mine.
abstract: Strain NL19T is a Gram-stain-negative, aerobic bacterium that was isolated from sludge of a deactivated uranium mine in Portugal. 16S rRNA gene sequence analysis revealed that strain NL19T is a member of the genus Pedobacter and closely related to the strains Pedobacter himalayensis MTCC 6384T, Pedobacter cryoconitis DSM 14825T, Pedobacter westerhofensis DSM 19036T and Pedobacterhartonius DSM 19033T. It had a DNA G+C content of 40.8 mol%, which agreed with the genus description. The main fatty acids included C16 : 1ω7c, C14 : 1ω5c, C4 : 0, iso-C17 : 0, iso-C17 : 0 3-OH, C16 : 0, anteiso-C15 : 0 and iso-C15 : 0 3-OH. The main lipids present were phospholipids (60 %) and sphingolipids (35 %). The most abundant phospholipids included phosphatidylethanolamine, phosphatidylinositol and phosphatidylcholine. Menaquinone-7 (MK-7) was the only isoprenoid quinone detected. DNA-DNA hybridization similarities between strain NL19T and Pedobacter himalayensis MTCC 6384T, Pedobacter cryoconitis DSM 14825T, Pedobacter westerhofensis DSM 19036T and Pedobacter hartonius DSM 19033T were 15.3 , 16.2 , 11.5 and 16.0 %, respectively. Strain NL19T can also be distinguished from these four species based on gyrB and intergenic transcribed spacers (ITS) sequences and by some phenotypic traits such as NaCl tolerance, pH, growth temperature and carbon source utilization. Strain NL19Trepresents a novel species of the genus Pedobacter, for which the name Pedobacter lusitanus sp. nov. is proposed. The type strain is NL19T (=LMG 29220T=CECT 9028T). An amended description of Pedobacter himalayensis is also included.
authors.affiliation: Biology Department and CESAM, University of Aveiro, Aveiro, Portugal.
authors.forename: Cláudia
authors.initials: C
authors.lastname: Covas
authors.affiliation: Biology Department and CESAM, University of Aveiro, Aveiro, Portugal.
authors.forename: Tânia
authors.initials: T
authors.lastname: Caetano
authors.affiliation: Biology Department and CESAM, University of Aveiro, Aveiro, Portugal.
authors.forename: Andreia
authors.initials: A
authors.lastname: Cruz
authors.affiliation: Biology Department and CESAM, University of Aveiro, Aveiro, Portugal.
authors.forename: Tiago
authors.initials: T
authors.lastname: Santos
authors.affiliation: Biology Department and CESAM, University of Aveiro, Aveiro, Portugal.
authors.forename: Liliana
authors.initials: L
authors.lastname: Dias
authors.affiliation: Institute of Food Quality and Food Safety, Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Guenter
authors.initials: G
authors.lastname: Klein
authors.affiliation: Institute of Food Quality and Food Safety, Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Amir
authors.initials: A
authors.lastname: Abdulmawjood
authors.affiliation: CBQF - Centro de Biotecnologia e Quı́mica Fina - Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Porto, Portugal.|Centro de Investigación en Recursos Naturales y Sustentabilidad (CIRENYS), Universidad Bernardo O'Higgins, Fábrica N∘ 1990, Segundo Piso, Santiago, Chile.
authors.forename: Luis M
authors.initials: LM
authors.lastname: Rodríguez-Alcalá
authors.affiliation: CBQF - Centro de Biotecnologia e Quı́mica Fina - Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Porto, Portugal.
authors.forename: Lígia L
authors.initials: LL
authors.lastname: Pimentel
authors.affiliation: CBQF - Centro de Biotecnologia e Quı́mica Fina - Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Porto, Portugal.
authors.forename: Ana
authors.initials: A
authors.lastname: Gomes
authors.affiliation: CBQF - Centro de Biotecnologia e Quı́mica Fina - Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Porto, Portugal.
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Freitas
authors.affiliation: Department of Bioactivity and Food Analysis, Group of Lipids, Instituto de Investigación en Ciencias de la Alimentación (CIAL CSIC-UAM), Universidad Autónoma de Madrid, Nicolás Cabrera 9, Madrid 28049, Spain.
authors.forename: Alba
authors.initials: A
authors.lastname: Garcia-Serrano
authors.affiliation: Department of Bioactivity and Food Analysis, Group of Lipids, Instituto de Investigación en Ciencias de la Alimentación (CIAL CSIC-UAM), Universidad Autónoma de Madrid, Nicolás Cabrera 9, Madrid 28049, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Fontecha
authors.affiliation: Biology Department and CESAM, University of Aveiro, Aveiro, Portugal.
authors.forename: Sónia
authors.initials: S
authors.lastname: Mendo
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D008906:Mining; D009693:Nucleic Acid Hybridization; D060485:Pedobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D011174:Portugal; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D013107:Sphingolipids; D014501:Uranium; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D013107:Sphingolipids; D024482:Vitamin K 2; D014501:Uranium; C062629:menaquinone 7