title: Pulsed light and antimicrobial combination treatments for surface decontamination of cheese: Favorable and antagonistic effects.
abstract: Postprocessing cross-contamination of cheese can lead to both food safety issues and significant losses due to spoilage. Pulsed light (PL) treatment, consisting of short, high-energy, broad-spectrum light pulses, has been proven effective in reducing the microbial load on cheese surface. As PL treatment effectiveness is limited by light-cheese interactions, the possibility to improve its effectiveness by combining it with the antimicrobial nisin was explored. The effect of natamycin, which is added to cheeses as an antifungal agent, on PL effectiveness was also investigated. Pseudomonas fluorescens, Escherichia coli ATCC 25922, and Listeria innocua were used as challenge microorganisms. Bacterial cultures in stationary growth phase were diluted to initial inoculum levels of 5 or 7 log cfu per cheese slice. Slices of sharp white Cheddar cheese and white American singles were cut in rectangles of 2.5 × 5 cm. For cheese slices receiving antimicrobial treatment before PL, slices were dipped in natamycin or nisin, spot inoculated with 100 μL of bacterial suspension, and then treated with PL. Cheese slices receiving PL treatment before antimicrobials were spot inoculated, treated with PL, and then treated with antimicrobials. The PL fluence levels from 1.02 to 12.29 J/cm2were used. Survivors were enumerated by standard plate counting or the most probable number technique, as appropriate. All treatments were performed in triplicate, and the data were analyzed using a general linear model. Treatment with nisin or natamycin before PL decreased the effectiveness of PL for all bacteria tested. For instance, PL reduced P. fluorescens on Cheddar cheese by 2.19 ± 0.27 log after 6.14 J/cm2, whereas combination treatments at the same PL fluence yielded barely 1 log reduction. Inactivation of L. innocua on Cheddar was only 0.78 ± 0.01 log when using PL after nisin, compared with a 1.30 ± 0.76 log reduction by nisin alone. This was attributed to the absorption of UV light by the 2 antimicrobials, which diminished the UV fluence received by the bacteria. Increased inactivation was obtained when antimicrobials were applied after PL. On process cheese, a maximum reduction of 3.73 ± 0.96 log of L. innocua was obtained at 9.22 J/cm2for PL followed by nisin, compared with 3.01 ± 0.48 by PL alone. This study demonstrates that antimicrobials may increase the antimicrobial effectiveness of PL on cheese surface, but the order of treatments is critical.
authors.affiliation: Department of Food Science, Cornell University, Ithaca, New York 14853.
authors.forename: J
authors.initials: J
authors.lastname: Proulx
authors.affiliation: Department of Food Science, Cornell University, Ithaca, New York 14853.
authors.forename: G
authors.initials: G
authors.lastname: Sullivan
authors.affiliation: Department of Food Science, Cornell University, Ithaca, New York 14853.
authors.forename: L F
authors.initials: LF
authors.lastname: Marostegan
authors.affiliation: Department of Food Science, Cornell University, Ithaca, New York 14853.
authors.forename: S
authors.initials: S
authors.lastname: VanWees
authors.affiliation: Department of Food Science, Cornell University, Ithaca, New York 14853.
authors.forename: L C
authors.initials: LC
authors.lastname: Hsu
authors.affiliation: Department of Food Science, Cornell University, Ithaca, New York 14853. Electronic address: cim24@cornell.edu.
authors.forename: C I
authors.initials: CI
authors.lastname: Moraru
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents; D002611:Cheese / Q000382:microbiology*; D015169:Colony Count, Microbial; D003666:Decontamination; D005516:Food Microbiology; D009561:Nisin
chemical_list: D000890:Anti-Infective Agents; D009561:Nisin
keywords: cheese surface decontamination; natamycin; nisin; pulsed light