title: Identification of novel cellular clusters define a specialized area in the cerebellar periventricular zone.
abstract: The periventricular zone of cerebellum is a germinative niche during the embryonic development, nevertheless its structural organization and functional implications in adult have not been widely studied. Here we disclose the presence of two novel clusters of cells in that area. The first one was named the subventricular cellular cluster (SVCC) and is composed of cells that express glial and neuronal markers. The second was named the ventromedial cord (VMC) and appears as a streak of biciliated cells with microvillosities facing the ventricle, that includes GFAP+and nestin+cells organized along the periventricular vasculature. The dorsal limit of the SVCC is associated with myelinated axons of neurons of unknown origin. This paper describes the characteristics and organization of these groups of cells. They can be observed from late embryonic development in the transgenic mouse line GFAP-GFP. The SVCC and VMC expand during early postnatal development but are restricted to the central area of the ventricle in adulthood. We did not find evidence of cell proliferation, cell migration or the presence of fenestrated blood vessels. These findings provide new insights into the knowledge of the cellular composition and structural organization of the periventricular zone of cerebellum.
authors.affiliation: Instituto de Neurobiología, Universidad Nacional Autónoma de México, Departamento de Neurobiología Celular y Molecular, Laboratorio de Neurobiología Molecular y Celular, Juriquilla, Querétaro, 76230, México.
authors.forename: María Alejandra
authors.initials: MA
authors.lastname: González-González
authors.affiliation: Instituto de Neurobiología, Universidad Nacional Autónoma de México, Departamento de Neurobiología Celular y Molecular, Laboratorio de Neurobiología Molecular y Celular, Juriquilla, Querétaro, 76230, México.
authors.forename: Gabriela B
authors.initials: GB
authors.lastname: Gómez-González
authors.affiliation: Instituto de Neurobiología, Universidad Nacional Autónoma de México, Departamento de Neurobiología Celular y Molecular, Laboratorio de Neurobiología Molecular y Celular, Juriquilla, Querétaro, 76230, México.
authors.forename: Marymar
authors.initials: M
authors.lastname: Becerra-González
authors.affiliation: Instituto de Neurobiología, Universidad Nacional Autónoma de México, Departamento de Neurobiología Celular y Molecular, Laboratorio de Neurobiología Molecular y Celular, Juriquilla, Querétaro, 76230, México.
authors.forename: Ataúlfo
authors.initials: A
authors.lastname: Martínez-Torres
mesh_terms: D000818:Animals; D015415:Biomarkers; D049109:Cell Proliferation; D002531:Cerebellum / Q000166:cytology* / Q000378:metabolism* / Q000503:physiopathology / Q000648:ultrastructure; D055724:Electrophysiological Phenomena; D005455:Fluorescent Antibody Technique; D017930:Genes, Reporter; D007031:Hypothalamus / Q000166:cytology* / Q000378:metabolism* / Q000503:physiopathology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D018408:Patch-Clamp Techniques
chemical_list: D015415:Biomarkers