title: Changes in the Neurochemical Composition of Motor Neurons of the Spinal Cord in Mice under Conditions of Space Flight.
abstract: Expression of choline acetyltransferase, 200-kDa neurofilament protein, 28-kDa calbindin, neuronal NO synthase, caspase 3, and Ki-67 in the motor neurons of spinal cord segments T3-T5 in male C57Bl/6 mice after 30-day space flight in the Bion-M1 biosatellite was studied by immunohistochemical methods. Under conditions space flight, the size of motoneurons increased, the number of neurons containing choline acetyltransferase and neurofilaments, decreased, and the number of calbindin-positive neurons increased; motoneurons, expressing neuronal NO synthase and caspase 3 appeared, while Ki-67 was not detected. Fragmentation of neurons with the formation structures similar to apoptotic (residual) bodies was observed in individual caspase 3-positive motoneurons.
authors.affiliation: Department of Normal Physiology and Biophysics, Yaroslavl State Medical University, Ministry of Health of the Russian Federation, Yaroslavl, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Porseva
authors.affiliation: Department of Normal Physiology and Biophysics, Yaroslavl State Medical University, Ministry of Health of the Russian Federation, Yaroslavl, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Shilkin
authors.affiliation: Department of Normal Physiology and Biophysics, Yaroslavl State Medical University, Ministry of Health of the Russian Federation, Yaroslavl, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Strelkov
authors.affiliation: Laboratory of Gravitational Biology, State Scientific Centre Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia.
authors.forename: I B
authors.initials: IB
authors.lastname: Krasnov
authors.affiliation: Department of Normal Physiology and Biophysics, Yaroslavl State Medical University, Ministry of Health of the Russian Federation, Yaroslavl, Russia. mpm@yma.ac.ru.
authors.forename: P M
authors.initials: PM
authors.lastname: Masliukov
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D064026:Calbindins / Q000235:genetics / Q000378:metabolism; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002452:Cell Count; D048429:Cell Size; D002795:Choline O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D009046:Motor Neurons / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D016900:Neurofilament Proteins / Q000235:genetics / Q000378:metabolism; D052248:Nitric Oxide Synthase Type I / Q000235:genetics / Q000378:metabolism; D013026:Space Flight; D013116:Spinal Cord / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D014893:Weightlessness
chemical_list: D064026:Calbindins; D019394:Ki-67 Antigen; C498355:Mki67 protein, mouse; D016900:Neurofilament Proteins; C051774:neurofilament protein H; D052248:Nitric Oxide Synthase Type I; C496321:Nos1 protein, mouse; D002795:Choline O-Acetyltransferase; C505409:Casp3 protein, mouse; D053148:Caspase 3
keywords: immunohistochemistry; microgravity; motoneurons; spinal cord