title: Fluorogenic probes to monitor cytosolic phospholipase A2activity.
abstract: Arachidonic acid derivatives equipped with either one or two fluorescent groups attached to the tip of the alkyl chains were synthesized and shown to function as inhibitor and substrate probes of cPLA2. The inhibitor probe was demonstrated to perform dual functions of inhibition and imaging while the substrate probe could be used for activity assay.
authors.affiliation: Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore. chmlamyl@nus.edu.sg.
authors.forename: Cheng Yang
authors.initials: CY
authors.lastname: Ng
authors.affiliation: Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore. chmlamyl@nus.edu.sg.
authors.forename: Timothy Xiong Wei
authors.initials: TX
authors.lastname: Kwok
authors.affiliation: Department of Anaesthesia, Yong Loo Lin School of Medicine, National University of Singapore, 5 Lower Kent Ridge Road, Singapore 119074, Singapore.
authors.forename: Francis Chee Kuan
authors.identifier: http://orcid.org/0000-0002-5272-9990
authors.initials: FC
authors.lastname: Tan
authors.affiliation: Department of Anaesthesia, Yong Loo Lin School of Medicine, National University of Singapore, 5 Lower Kent Ridge Road, Singapore 119074, Singapore and Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, MD3 #04-01V, 16 Medical Drive, Singapore 117600, Singapore. phclowcm@nus.edu.sg.
authors.forename: Chian-Ming
authors.initials: CM
authors.lastname: Low
authors.affiliation: Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore. chmlamyl@nus.edu.sg.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Lam
mesh_terms: D016718:Arachidonic Acid / Q000378:metabolism; D002460:Cell Line; D003600:Cytosol / Q000201:enzymology*; D005456:Fluorescent Dyes / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D054467:Phospholipases A2 / Q000032:analysis* / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D016718:Arachidonic Acid; D054467:Phospholipases A2