title: Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels.
abstract: Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity.
authors.affiliation: Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: T W
authors.initials: TW
authors.lastname: Bilverstone
authors.affiliation: Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, NG7 2RD, UK; NIHR Nottingham Digestive Diseases, (NDCC) Biomedical Research Unit, Nottingham University Hospitals NHS Trust and the University of Nottingham, NG7 2RD, UK.
authors.forename: N L
authors.initials: NL
authors.lastname: Kinsmore
authors.affiliation: Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, NG7 2RD, UK; NIHR Nottingham Digestive Diseases, (NDCC) Biomedical Research Unit, Nottingham University Hospitals NHS Trust and the University of Nottingham, NG7 2RD, UK. Electronic address: nigel.minton@nottingham.ac.uk.
authors.forename: N P
authors.initials: NP
authors.lastname: Minton
authors.affiliation: Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, NG7 2RD, UK; NIHR Nottingham Digestive Diseases, (NDCC) Biomedical Research Unit, Nottingham University Hospitals NHS Trust and the University of Nottingham, NG7 2RD, UK.
authors.forename: S A
authors.initials: SA
authors.lastname: Kuehne
mesh_terms: D036002:ADP Ribose Transferases / Q000378:metabolism* / Q000633:toxicity; D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism* / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D016360:Clostridioides difficile / Q000235:genetics* / Q000378:metabolism*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D005809:Genes, Regulator; D014709:Vero Cells
chemical_list: D001426:Bacterial Proteins; D036002:ADP Ribose Transferases; C410164:actin-specific ADP-ribosyltransferase, Clostridium
keywords: Binary toxin; C. difficile; CdtR; PaLoc; Virulence