title: Intervention of Isomaltodextrin Mitigates Intestinal Inflammation in a Dextran Sodium Sulfate-Induced Mouse Model of Colitis via Inhibition of Toll-like Receptor-4.
abstract: Isomaltodextrin (IMD), a highly branched α-glucan, is a type of resistant starch. Earlier studies have indicated that polysaccharides could prevent inflammation and can be effective in reducing the complications of chronic gastrointestinal diseases such as inflammatory bowel disease (IBD). Therefore, the aim of the present study was to evaluate the anti-inflammatory effect of IMD in dextran sodium sulfate (DSS)-induced colitis in a mouse model. IMD (0.5, 1.0, 2.5, and 5.0% (w/v)) was given orally for 23 days to female Balb/c mice, and then 5% DSS was administered to induce colitis (from day 15 onward to the end of the trial). IMD could not prevent DSS-induced weight loss or colon shortening. However, IMD could reduce inflammatory cytokines, TNF-α and IL-6, in the colon. Gene expression indicated the tendency of IMD to suppress pro-inflammatory cytokines IL-1β, MCP-1, and IL-17 and to increase an anti-inflammatory cytokine, IL-10. Further study revealed that the anti-inflammatory action of IMD mediates through inhibition of the expression of Toll-like receptor-4.
authors.affiliation: Department of Food Science, University of Guelph , Guelph, Ontario N1G 2W1, Canada.
authors.forename: Kaustav
authors.initials: K
authors.lastname: Majumder
authors.affiliation: Department of Food Science, University of Guelph , Guelph, Ontario N1G 2W1, Canada.|Division of Bioresources and Bioenvironmental Sciences, Faculty of Agriculture, Graduate School, Kyushu University , Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Fukuda
authors.affiliation: Department of Food Science, University of Guelph , Guelph, Ontario N1G 2W1, Canada.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.affiliation: R&D Center, Hayashibara Company, Ltd. , 675-1 Fujisaki, Naka-ku, Okayama 702-8006, Japan.
authors.forename: Takeo
authors.initials: T
authors.lastname: Sakurai
authors.affiliation: R&D Center, Hayashibara Company, Ltd. , 675-1 Fujisaki, Naka-ku, Okayama 702-8006, Japan.
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Taniguchi
authors.affiliation: R&D Center, Hayashibara Company, Ltd. , 675-1 Fujisaki, Naka-ku, Okayama 702-8006, Japan.
authors.forename: Hikaru
authors.initials: H
authors.lastname: Watanabe
authors.affiliation: R&D Center, Hayashibara Company, Ltd. , 675-1 Fujisaki, Naka-ku, Okayama 702-8006, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Mitsuzumi
authors.affiliation: Division of Bioresources and Bioenvironmental Sciences, Faculty of Agriculture, Graduate School, Kyushu University , Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Toshiro
authors.initials: T
authors.lastname: Matsui
authors.affiliation: Department of Food Science, University of Guelph , Guelph, Ontario N1G 2W1, Canada.
authors.forename: Yoshinori
authors.identifier: http://orcid.org/0000-0002-3567-5556
authors.initials: Y
authors.lastname: Mine
mesh_terms: D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology; D003106:Colon / Q000276:immunology; D016264:Dextran Sulfate / Q000009:adverse effects; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology; D007422:Intestines / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011134:Polysaccharides / Q000008:administration & dosage*; D013431:Sulfates / Q000009:adverse effects; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology
chemical_list: D015850:Interleukin-6; D011134:Polysaccharides; D013431:Sulfates; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; C012036:sodium sulfate; D016753:Interleukin-10; C008315:maltodextrin; D016264:Dextran Sulfate
keywords: Toll-like receptor-4; dextran sodium sulfate (DSS)-induced colitis; inflammatory bowel disease; inflammatory cytokine; isomaltodextrin