title: Design an aptasensor based on structure-switching aptamer on dendritic gold nanostructures/Fe3O4@SiO2/DABCO modified screen printed electrode for highly selective detection of epirubicin.
abstract: The present work describes a label free electrochemical aptasensor for selective detection of epirubicin. In this project, 5'-thiole terminated aptamer was self-assembled on carbon screen printed electrode, which modified with electrodeposited gold nanoparticles on magnetic double-charged diazoniabicyclo [2.2.2] octane dichloride silica hybrid (Fe3O4@SiO2/DABCO) by Au-S bond. The interactions of epirubicin with aptamer on the AuNPs/Fe3O4@SiO2/DABCO/SPE have been studied by cyclic voltammetry, linear sweep voltammetry and electrochemical impedance spectroscopy. Under optimized conditions, the peak current of epirubicin increased linearly with increasing epirubicin concentration, due to the switching in the aptamer conformation and formation of aptamer- epirubicin complex instead of aptamer on the modified electrode surface. The Apt/AuNPs/Fe3O4@SiO2/DABCO/SPE is sensitive, selective and has two linear range from 0.07µM to 1.0µM and 1.0µM to 21.0µM with a detection limit of 0.04µM. The applicability of the aptasensor was successfully assessed by determination of epirubicin in a human blood serum sample.
authors.affiliation: Electroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
authors.forename: Ayemeh Bagheri
authors.initials: AB
authors.lastname: Hashkavayi
authors.affiliation: Electroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran. Electronic address: j.raoof@umz.ac.ir.
authors.forename: Jahan Bakhsh
authors.initials: JB
authors.lastname: Raoof
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000097:blood*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D058266:Dielectric Spectroscopy / Q000295:instrumentation / Q000379:methods; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D004595:Electroplating; D015251:Epirubicin / Q000097:blood*; D052203:Ferrosoferric Oxide / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010879:Piperazines / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D000903:Antibiotics, Antineoplastic; D052157:Aptamers, Nucleotide; D010879:Piperazines; D015251:Epirubicin; D006046:Gold; D012822:Silicon Dioxide; C007306:triethylenediamine; D052203:Ferrosoferric Oxide
keywords: Aptamer; Carbon screen-printed electrode; Dendritic gold nanostructure; Epirubicin