title: Mangrovibacter phragmitis sp. nov., an endophyte isolated from the roots of Phragmites karka.
abstract: A facultatively anaerobic, Gram-stain-negative, rod-shaped, nitrogen-fixing, endophytic bacterial strain designated MP23T was isolated from the roots of Phragmites karka growing in Chilika Lagoon, Odisha, India. Strain MP23T was slightly halophilic, and the optimal NaCl concentration and temperature for growth were 1 % and 30 °C, respectively. On the basis of 16S rRNA gene sequence similarities, strain MP23T was affiliated to the family Enterobacteriaceae and most closely related to Mangrovibacter yixingensis KCTC 42181T and Mangrovibacter plantisponsor DSM 19579T with 99.71 % similarity, followed by Salmonella enterica subsp. salamae DSM 9220T (97.22 %), Cronobacter condimenti LMG 26250T (97.14 %) and Salmonella enterica subsp. diarizonae DSM 14847T (97 %). Sequence analysis of 16S rRNA, hsp60, gyrB and rpoB genes showed that strain MP23T formed a phylogenetic cluster with M. yixingensis KCTC 42181T and M. plantisponsor DSM 19579T indicating that it belongs to the genus Mangrovibacter. The major cellular fatty acids were C16 : 0, C18 : 1ω6c and/or C18 : 1ω7c, C16 : 1ω6c and/or C16 : 1ω7c, C14 : 0, C14 : 0 3-OH and/or iso-C16 : 1 I and C17 : 0 cyclo. Polar lipids of strain MP23T consisted of phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 50.3 mol%. Based on experimental DNA-DNA hybridization values and average nucleotide identity derived from in silico comparison of whole-genome sequences, strain MP23T could be distinguished from its closest neighbours. We therefore conclude that strain MP23T represents a novel species of the genus Mangrovibacter for which the name Mangrovibacter phragmitis sp. nov. is proposed. The type strain is MP23T (=DSM 100250T=KCTC 42580T).
authors.affiliation: Wetland Research and Training Centre, Chilika Development Authority Odisha, Barkul, Balugaon 752030, India.|School of Biotechnology, KIIT University, Patia, Bhubaneswar 751024, Odisha, India.
authors.forename: Pratiksha
authors.initials: P
authors.lastname: Behera
authors.affiliation: Microbial Culture Collection, Pashan, Pune, Maharashtra 411021, India.
authors.forename: V
authors.initials: V
authors.lastname: Venkata Ramana
authors.affiliation: Wetland Research and Training Centre, Chilika Development Authority Odisha, Barkul, Balugaon 752030, India.
authors.forename: Bhagirathi
authors.initials: B
authors.lastname: Maharana
authors.affiliation: Microbial Culture Collection, Pashan, Pune, Maharashtra 411021, India.
authors.forename: Neetha
authors.initials: N
authors.lastname: Joseph
authors.affiliation: Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, NASA, California Institute of Technology, Pasadena, CA 91109, USA.
authors.forename: Parag
authors.initials: P
authors.lastname: Vaishampayan
authors.affiliation: Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, NASA, California Institute of Technology, Pasadena, CA 91109, USA.
authors.forename: Nitin K
authors.initials: NK
authors.lastname: Singh
authors.affiliation: Microbial Culture Collection, Pashan, Pune, Maharashtra 411021, India.
authors.forename: Yogesh
authors.initials: Y
authors.lastname: Shouche
authors.affiliation: Integrative Taxonomy and Microbial Ecology Research Group, Department of Biological Sciences, Indian Institute of Science Education and Research- Kolkata, Mohanpur 741252, Nadia, West Bengal, India.
authors.forename: Punyasloke
authors.initials: P
authors.lastname: Bhadury
authors.affiliation: School of Biotechnology, KIIT University, Patia, Bhubaneswar 751024, Odisha, India.
authors.forename: Samir R
authors.initials: SR
authors.lastname: Mishra
authors.affiliation: School of Biotechnology, KIIT University, Patia, Bhubaneswar 751024, Odisha, India.
authors.forename: Vishakha
authors.initials: V
authors.lastname: Raina
authors.affiliation: School of Biotechnology, KIIT University, Patia, Bhubaneswar 751024, Odisha, India.
authors.forename: Mrutyunjay
authors.initials: M
authors.lastname: Suar
authors.affiliation: Wetland Research and Training Centre, Chilika Development Authority Odisha, Barkul, Balugaon 752030, India.|Present address: State Project Management Unit, Integrated Coastal Zone Management Project, Bhubaneswar, Odisha 752003, India.
authors.forename: Ajit K
authors.initials: AK
authors.lastname: Pattnaik
authors.affiliation: Wetland Research and Training Centre, Chilika Development Authority Odisha, Barkul, Balugaon 752030, India.
authors.forename: Gurdeep
authors.initials: G
authors.lastname: Rastogi
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D060026:Endophytes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D004755:Enterobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005227:Fatty Acids / Q000737:chemistry; D007194:India; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D006109:Poaceae / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S