title: Detection of aberrant methylated SEPT9 and NTRK3 genes in sporadic colorectal cancer patients as a potential diagnostic biomarker.
abstract: Colorectal cancer (CRC) is one of the most common malignancies, and the third leading cause of cancer mortality worldwide. Timely detection of CRC in patients with earlier stages provides the highest rate of survival. Epigenetic alterations are important in the occurrence and progression of CRC, and represent the primary modifications of cancer cells. Therefore, detection of these alterations in CRC cases are thought to hold great promise as diagnostic biomarkers. It has been shown that theSEPT9andNTRK3genes are aberrantly methylated and their detection can be used as biomarkers for early diagnosis of CRC. The present study analyzed promoter methylation status of these genes in CRC patients. Genomic DNA was extracted from 45 CRC and paired adjacent healthy tissues and undergone bisulfite conversion, and the methylation status ofNTRK3andSEPT9were defined using the MS-HRM assay. Our results showed that there are statistically significant differences in methylation status ofNTRK3and speciallySEPT9between CRC and adjacent normal tissues (P<0.001). High sensitivity and specificity for a specific location inSEPT9gene promoter as a diagnostic biomarker was observed.SEPT9promoter hypermethylation may serve as a promising biomarker for the detection of CRC development. However, to validate the biomarker potential ofNTRK3there is a requirement for further investigation.
authors.affiliation: Department of Biochemistry and Clinical Laboratory, Division of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz 5165665811, Iran; Department of Molecular Medicine, Pasteur Institute of Iran, Tehran 1316943551, Iran; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz 5166614731, Iran.
authors.forename: Shahin
authors.initials: S
authors.lastname: Behrouz Sharif
authors.affiliation: Department of General & Vascular Surgery, Tabriz University of Medical Sciences, Tabriz 5165665811, Iran; Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz 5165665811, Iran.
authors.forename: Shahriar
authors.initials: S
authors.lastname: Hashemzadeh
authors.affiliation: Department of Biochemistry and Clinical Laboratory, Division of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz 5165665811, Iran; Department of Molecular Medicine, Pasteur Institute of Iran, Tehran 1316943551, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Mousavi Ardehaie
authors.affiliation: Department of Pathology, Imam Reza Hospital, Tabriz University of Medical Sciences, Tabriz 5165665811, Iran.
authors.forename: Amirtaher
authors.initials: A
authors.lastname: Eftekharsadat
authors.affiliation: Liver and Gastrointestinal Disease Research Center and Department of General and Thoracic Surgery, Tabriz University of Medical Sciences, Tabriz 5165665811, Iran.
authors.forename: Mortaza
authors.initials: M
authors.lastname: Ghojazadeh
authors.affiliation: Department of Molecular Medicine, Pasteur Institute of Iran, Tehran 1316943551, Iran.
authors.forename: Amir Hossein
authors.initials: AH
authors.lastname: Mehrtash
authors.affiliation: Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran 1471613151, Iran.
authors.forename: Mehrdad Asghari
authors.initials: MA
authors.lastname: Estiar
authors.affiliation: Department of Molecular Medicine, Pasteur Institute of Iran, Tehran 1316943551, Iran.
authors.forename: Ladan
authors.initials: L
authors.lastname: Teimoori-Toolabi
authors.affiliation: Department of Biochemistry and Clinical Laboratory, Division of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz 5165665811, Iran; Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz 5165665811, Iran.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Sakhinia
keywords: NTRK3; SEPT9; biomarker; colorectal cancer; methylation