title: BKV-specific T cells in the treatment of severe refractory haemorrhagic cystitis after HLA-haploidentical haematopoietic cell transplantation.
abstract: BACKGROUND
  Haemorrhagic cystitis caused by BK virus (BKV) is a known complication of allogeneic haematopoietic cell transplantation (HCT) and is relatively common following HLA-haploidentical transplantation. Adoptive immunotransfer of virus-specific T cells from the donor is a promising therapeutic approach, although production of these cells is challenging, particularly when dealing with low-frequency T cells such as BKV-specific T cells.
  
  
  METHODS
  Here, we present a patient who, following haploidentical HCT, developed severe BKV haemorrhagic cystitis, resistant to standard therapy. He responded well to adoptive transfer of donor cells enriched in BKV-specific T cells using the new second-generation CliniMACS Prodigy and the Cytokine Capture System from Miltenyi Biotec. Treatment led to full resolution of both the symptoms and viraemia without unwanted complications.
  
  
  CONCLUSIONS
  Our observations suggest that use of products enriched with BKV-specific T cells generated using this system is safe and efficient in HLA-haploidentical HCT where BKV cystitis can be a serious complication.
authors.affiliation: Centre for Haematology, Imperial College at Hammersmith Hospital, London, UK.
authors.forename: Oscar M
authors.identifier: http://orcid.org/0000-0002-6519-2598
authors.initials: OM
authors.lastname: Pello
authors.affiliation: Centre for Haematology, Imperial College at Hammersmith Hospital, London, UK.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Innes
authors.affiliation: Centre for Haematology, Imperial College at Hammersmith Hospital, London, UK.
authors.forename: Anne
authors.initials: A
authors.lastname: Bradshaw
authors.affiliation: Centre for Haematology, Imperial College at Hammersmith Hospital, London, UK.
authors.forename: Sally-Anne
authors.initials: SA
authors.lastname: Finn
authors.affiliation: Centre for Haematology, Imperial College at Hammersmith Hospital, London, UK.
authors.forename: Shab
authors.initials: S
authors.lastname: Uddin
authors.affiliation: Centre for Haematology, Imperial College at Hammersmith Hospital, London, UK.
authors.forename: Emma
authors.initials: E
authors.lastname: Bray
authors.affiliation: Centre for Haematology, Imperial College at Hammersmith Hospital, London, UK.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Olavarria
authors.affiliation: Centre for Haematology, Imperial College at Hammersmith Hospital, London, UK.
authors.forename: Jane F
authors.initials: JF
authors.lastname: Apperley
authors.affiliation: Centre for Haematology, Imperial College at Hammersmith Hospital, London, UK.
authors.forename: Jiří
authors.initials: J
authors.lastname: Pavlů
mesh_terms: D000328:Adult; D001739:BK Virus / Q000472:pathogenicity / Q000502:physiology; D003556:Cystitis / Q000209:etiology / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006470:Hemorrhage / Q000209:etiology / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D006650:Histocompatibility Testing; D006801:Humans; D016219:Immunotherapy, Adoptive; D015470:Leukemia, Myeloid, Acute / Q000276:immunology / Q000473:pathology / Q000628:therapy; D008297:Male; D027601:Polyomavirus Infections / Q000209:etiology / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D013601:T-Lymphocytes / Q000637:transplantation*; D014185:Transplantation, Isogeneic; D016896:Treatment Outcome; D014412:Tumor Virus Infections / Q000209:etiology / Q000276:immunology / Q000473:pathology / Q000628:therapy*
keywords: BK virus; CliniMACS Prodigy; HLA transplantation; adoptive transfer; case report; donor-specific T cells