title: Harnessing ester bond chemistry for protein ligation.
abstract: The hydrolysis potential of ester bonds in covalently cross-linking proteins is captured in our novel protein ligation technology. This new type of "molecular superglue" based on the spontaneously-formed Thr-Gln ester bonds found in cell-surface adhesins, affords a unique mechanism to both rationally assemble and disassemble complex protein nanomaterials.
authors.affiliation: School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland, New Zealand. c.squire@auckland.ac.nz and Maurice Wilkins Centre for Molecular Biodiscovery, c/o The University of Auckland, Private Bag 92019, Auckland, New Zealand.
authors.forename: P G
authors.initials: PG
authors.lastname: Young
authors.affiliation: School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland, New Zealand. c.squire@auckland.ac.nz.
authors.forename: Y
authors.initials: Y
authors.lastname: Yosaatmadja
authors.affiliation: School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland, New Zealand. c.squire@auckland.ac.nz and Maurice Wilkins Centre for Molecular Biodiscovery, c/o The University of Auckland, Private Bag 92019, Auckland, New Zealand.
authors.forename: P W R
authors.initials: PW
authors.lastname: Harris
authors.affiliation: School of Chemical Sciences, The University of Auckland, Private Bag 92019, Auckland, New Zealand.
authors.forename: I K H
authors.identifier: http://orcid.org/0000-0003-0633-6771
authors.initials: IK
authors.lastname: Leung
authors.affiliation: School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland, New Zealand. c.squire@auckland.ac.nz and Maurice Wilkins Centre for Molecular Biodiscovery, c/o The University of Auckland, Private Bag 92019, Auckland, New Zealand.
authors.forename: E N
authors.initials: EN
authors.lastname: Baker
authors.affiliation: School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland, New Zealand. c.squire@auckland.ac.nz and Maurice Wilkins Centre for Molecular Biodiscovery, c/o The University of Auckland, Private Bag 92019, Auckland, New Zealand.
authors.forename: C J
authors.identifier: http://orcid.org/0000-0001-9212-0461
authors.initials: CJ
authors.lastname: Squire
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry*; D003016:Clostridium perfringens / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004952:Esters / Q000737:chemistry*; D006868:Hydrolysis; D008958:Models, Molecular
chemical_list: D018829:Adhesins, Bacterial; D003432:Cross-Linking Reagents; D004952:Esters