title: Plasma miR-92a-2 as a biomarker for small cell lung cancer.
abstract: MicroRNAs (miRNAs) are small, non-coding RNAs that play important roles in the carcinogenesis and progression of cancers. Aberrant expression of miRNAs in tissue and plasma has been found in various solid tumors. Our research aims to determine whether the abnormal plasma miRNA expression patterns can be used as a predictive marker for the diagnosis and prognosis of small cell lung cancer (SCLC). Fifty SCLC patients and 30 healthy controls annotated with clinical characteristics and specific questionnaire survey for smoking history were available. Quantification of several miRNAs (miR-20a-5p, miR-92a-2-5p and miR-17-5p) was performed using quantitative real-time polymerase chain reaction (qRT-PCR), and results were analyzed using SPSS statistics 17.0. Plasma miR-92a-2 level was significantly higher in the SCLC patients group compared with healthy control (P< 0.0001), the receiver operating characteristic (ROC) curve analysis showed that the specificity and sensitivity were at 100% and 56% for diagnosis of SCLC, area under the ROC curve (AUC) was 0.761. No other statistically significant differences were found in the expression level of plasma miR-92a-2 among survival analysis in SCLC. Detection of miR-92a-2 levels in plasma could be a potential and noninvasive method for the diagnosis of SCLC.
authors.affiliation: Department of Laboratory Medicine, Clinical Laboratory Medicine and Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China.|Department of Laboratory Medicine, Clinical Laboratory Medicine and Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China.
authors.forename: Yalan
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Laboratory Medicine, Clinical Laboratory Medicine and Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China.|Department of Laboratory Medicine, Maternal and Child Health Hospital of Yiling, Yichang, Hubei, China.|Department of Laboratory Medicine, Clinical Laboratory Medicine and Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China.
authors.forename: Jiangcheng
authors.initials: J
authors.lastname: Zuo
authors.affiliation: Department of Laboratory Medicine, Clinical Laboratory Medicine and Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Tan
authors.affiliation: Department of Laboratory Medicine, Clinical Laboratory Medicine and Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China.
authors.forename: Khaing
authors.initials: K
authors.lastname: Zar Thin
authors.affiliation: Division of the Tumor Radiation and Chemotherapy, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Laboratory Medicine, Clinical Laboratory Medicine and Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China.
authors.forename: Man
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Department of Laboratory Medicine, Clinical Laboratory Medicine and Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China.|School of Laboratory Medicine, Hubei University of Traditional Chinese Medicine, Wuhan, Hubei, China.
authors.forename: Mingxia
authors.initials: M
authors.lastname: Yu
authors.affiliation: Department of Laboratory Medicine, Clinical Laboratory Medicine and Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China.
authors.forename: Zhenming
authors.initials: Z
authors.lastname: Fu
authors.affiliation: Department of Laboratory Medicine, Clinical Laboratory Medicine and Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China.
authors.forename: Chunzi
authors.initials: C
authors.lastname: Liang
authors.affiliation: Department of Laboratory Medicine, Clinical Laboratory Medicine and Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China.|School of Laboratory Medicine, Hubei University of Traditional Chinese Medicine, Wuhan, Hubei, China.
authors.forename: Jiancheng
authors.initials: J
authors.lastname: Tu
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality*; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D011379:Prognosis; D012372:ROC Curve; D012307:Risk Factors; D055752:Small Cell Lung Carcinoma / Q000097:blood / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality*; D016019:Survival Analysis
chemical_list: D014408:Biomarkers, Tumor; C541874:MIRN92 microRNA, human; D035683:MicroRNAs
keywords: MiR-92a-2; diagnosis; plasma; small cell lung cancer