title: The membrane-tethered NAC transcription factor, AtNTL7, contributes to ER-stress resistance in Arabidopsis.
abstract: We screened for endoplasmic reticulum (ER) stress-resistant mutants among 25 mutants of the Arabidopsis NTL (NAC with Transmembrane motif 1-Like) family. We identified a novel mutant, SALK_044777, showing strong resistance to ER stress. RT-PCR and genomic DNA sequence analyses identified the mutant as atntl7, which harbors a T-DNA insertion in the fourth exon of AtNTL7. Two other atntl7-mutant alleles, in which T-DNA was inserted in the second exon and third intron of AtNTL7, respectively, showed ER-stress sensitive phenotypes, suggesting that SALK_044777 is a gain-of-function mutant. Arabidopsis plants overexpressing AtNTL7 showed strong ER-stress resistance. Our findings suggest that AtNTL7 fragment is cleaved from the ER membrane under ER stress and translocates into the nucleus to induce downstream ER-stress responsive genes.
authors.affiliation: Division of Applied Life Science (BK21(+) Program) and PMBBRC, Gyeongsang National University, Jinju, 52828, Republic of Korea.
authors.forename: Yong Hun
authors.initials: YH
authors.lastname: Chi
authors.affiliation: Division of Applied Life Science (BK21(+) Program) and PMBBRC, Gyeongsang National University, Jinju, 52828, Republic of Korea.
authors.forename: Sarah Mae Boyles
authors.initials: SMB
authors.lastname: Melencion
authors.affiliation: Division of Applied Life Science (BK21(+) Program) and PMBBRC, Gyeongsang National University, Jinju, 52828, Republic of Korea.
authors.forename: Cresilda Vergara
authors.initials: CV
authors.lastname: Alinapon
authors.affiliation: Division of Applied Life Science (BK21(+) Program) and PMBBRC, Gyeongsang National University, Jinju, 52828, Republic of Korea.
authors.forename: Min Ji
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Division of Applied Life Science (BK21(+) Program) and PMBBRC, Gyeongsang National University, Jinju, 52828, Republic of Korea.
authors.forename: Eun Seon
authors.initials: ES
authors.lastname: Lee
authors.affiliation: Division of Applied Life Science (BK21(+) Program) and PMBBRC, Gyeongsang National University, Jinju, 52828, Republic of Korea.
authors.forename: Seol Ki
authors.initials: SK
authors.lastname: Paeng
authors.affiliation: Division of Applied Life Science (BK21(+) Program) and PMBBRC, Gyeongsang National University, Jinju, 52828, Republic of Korea.
authors.forename: Joung Hun
authors.initials: JH
authors.lastname: Park
authors.affiliation: Division of Applied Life Science (BK21(+) Program) and PMBBRC, Gyeongsang National University, Jinju, 52828, Republic of Korea.
authors.forename: Ganesh M
authors.initials: GM
authors.lastname: Nawkar
authors.affiliation: National Institute of Ecology, 1210 Geumgang-ro, Maseo-myeon, Seocheon-gun 33657, Republic of Korea.
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Jung
authors.affiliation: Division of Applied Life Science (BK21(+) Program) and PMBBRC, Gyeongsang National University, Jinju, 52828, Republic of Korea.
authors.forename: Ho Byoung
authors.initials: HB
authors.lastname: Chae
authors.affiliation: Division of Applied Life Science (BK21(+) Program) and PMBBRC, Gyeongsang National University, Jinju, 52828, Republic of Korea.
authors.forename: Chang Ho
authors.initials: CH
authors.lastname: Kang
authors.affiliation: Division of Applied Life Science (BK21(+) Program) and PMBBRC, Gyeongsang National University, Jinju, 52828, Republic of Korea. Electronic address: sylee@gnu.ac.kr.
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Lee
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D059865:Endoplasmic Reticulum Stress; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C000622961:NTL7 protein, Arabidopsis; D014157:Transcription Factors
keywords: ER stress; Membrane-tethered transcription factors; NAC-family transcription factor; Tunicamycin; Unfolded protein response