title: Anticancer activity of baicalein and luteolin studied in colorectal adenocarcinoma LoVo cells and in drug-resistant LoVo/Dx cells.
abstract: Due to the type-specific diversity of cancer cells, an analysis and elucidation of molecular mechanisms responsible for anticancer properties of biologically active compounds are essential. Plant-derived polyphenolic compounds such as flavonoids may be useful in cancer chemoprevention or treatment because they influence diverse molecular pathways in cancer cells. In these studies anticancer activity of natural occurring flavones, baicalein and luteolin was investigated in colon cancer cells LoVo and in their drug resistant subline LoVo/Dx. Inhibitory activity of these flavones on cells growth and their ability to induce apoptosis were observed. A less pronounced influence of studied flavones on proliferation and apoptosis of LoVo/Dx as compared with LoVo cells well correlated with significantly lower cytotoxicity of these compounds in drug-resistant cells. These effects may be related to overexpression of multidrug transporter P-glycoprotein in drug-resistant LoVo/Dx cells. Our studies indicated that baicalein could be a substrate of this drug transporter.
authors.affiliation: Department of Biophysics, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, Poland. Electronic address: labuz@umed.wroc.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Palko-Labuz
authors.affiliation: Department of Biophysics, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, Poland.
authors.forename: Kamila
authors.initials: K
authors.lastname: Sroda-Pomianek
authors.affiliation: Department of Biophysics, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Uryga
authors.affiliation: Department of Chemistry, Wroclaw University of Environmental and Life Sciences, Norwida 25, 50-375 Wroclaw, Poland.
authors.forename: Edyta
authors.initials: E
authors.lastname: Kostrzewa-Suslow
authors.affiliation: Department of Biophysics, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, Poland.
authors.forename: Krystyna
authors.initials: K
authors.lastname: Michalak
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D000230:Adenocarcinoma / Q000188:drug therapy*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015179:Colorectal Neoplasms / Q000188:drug therapy*; D019008:Drug Resistance, Neoplasm; D044950:Flavanones / Q000494:pharmacology*; D020868:Gene Silencing / Q000187:drug effects; D006801:Humans; D047311:Luteolin / Q000494:pharmacology*; D034741:RNA, Small Interfering / Q000494:pharmacology
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000972:Antineoplastic Agents, Phytogenic; D044950:Flavanones; D034741:RNA, Small Interfering; C006680:baicalein; C505407:CASP3 protein, human; D053148:Caspase 3; D047311:Luteolin
keywords: Caspase 3; Colon cancer; Flavonoids; Multidrug resistance; PCNA; RNAi