title: The Asymmetric Cell Division Regulators Par3, Scribble and Pins/Gpsm2 Are Not Essential for Erythroid Development or Enucleation.
abstract: Erythroid enucleation is the process by which the future red blood cell disposes of its nucleus prior to entering the blood stream. This key event during red blood cell development has been likened to an asymmetric cell division (ACD), by which the enucleating erythroblast divides into two very different daughter cells of alternate molecular composition, a nucleated cell that will be removed by associated macrophages, and the reticulocyte that will mature to the definitive erythrocyte. Here we investigated gene expression of members of the Par, Scribble and Pins/Gpsm2 asymmetric cell division complexes in erythroid cells, and functionally tested their role in erythroid enucleation in vivo and ex vivo. Despite their roles in regulating ACD in other contexts, we found that these polarity regulators are not essential for erythroid enucleation, nor for erythroid development in vivo. Together our results put into question a role for cell polarity and asymmetric cell division in erythroid enucleation.
authors.affiliation: Cell Cycle and Cancer Genetics, Peter MacCallum Cancer Centre, East Melbourne, Australia.|La Trobe Institute for Molecular Science, Department of Biochemistry and Genetics, La Trobe University, Melbourne, Australia.
authors.forename: Christina B
authors.initials: CB
authors.lastname: Wölwer
authors.affiliation: Cell Cycle and Cancer Genetics, Peter MacCallum Cancer Centre, East Melbourne, Australia.|La Trobe Institute for Molecular Science, Department of Biochemistry and Genetics, La Trobe University, Melbourne, Australia.
authors.forename: Nathan
authors.initials: N
authors.lastname: Gödde
authors.affiliation: Cell Cycle and Cancer Genetics, Peter MacCallum Cancer Centre, East Melbourne, Australia.
authors.forename: Luke B
authors.initials: LB
authors.lastname: Pase
authors.affiliation: Cell Cycle and Cancer Genetics, Peter MacCallum Cancer Centre, East Melbourne, Australia.
authors.forename: Imogen A
authors.initials: IA
authors.lastname: Elsum
authors.affiliation: La Trobe Institute for Molecular Science, Department of Biochemistry and Genetics, La Trobe University, Melbourne, Australia.
authors.forename: Krystle Y B
authors.initials: KY
authors.lastname: Lim
authors.affiliation: Immune Signaling Laboratory, Peter MacCallum Cancer Centre, East Melbourne, Australia.|Department of Pathology, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Faruk
authors.initials: F
authors.lastname: Sacirbegovic
authors.affiliation: St. Vincent's Institute of Medical Research, Fitzroy, Victoria, Australia.|Department of Medicine, St. Vincent's Hospital, The University of Melbourne, Fitzroy, Victoria.
authors.forename: Carl R
authors.initials: CR
authors.lastname: Walkley
authors.affiliation: Immune Signaling Laboratory, Peter MacCallum Cancer Centre, East Melbourne, Australia.|Department of Pathology, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Sarah
authors.initials: S
authors.lastname: Ellis
authors.affiliation: Department of Molecular Biology, Yokohama City University Graduate School of Medical Science, Yokohama, Japan.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Ohno
authors.affiliation: Laboratory for Cell Asymmetry, RIKEN Center for Developmental Biology, Kobe, Japan.
authors.forename: Fumio
authors.initials: F
authors.lastname: Matsuzaki
authors.affiliation: Department of Pathology, University of Melbourne, Parkville, Victoria, Australia.|Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, Australia.|Centre for Micro-Photonics, Faculty of Engineering and Industrial Sciences, Swinburne University of Technology, Hawthorn, Australia.
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Russell
authors.affiliation: Cell Cycle and Cancer Genetics, Peter MacCallum Cancer Centre, East Melbourne, Australia.|La Trobe Institute for Molecular Science, Department of Biochemistry and Genetics, La Trobe University, Melbourne, Australia.|Department of Pathology, University of Melbourne, Parkville, Victoria, Australia.|Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, Australia.|Department of Biochemistry and Molecular Biology, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Patrick O
authors.initials: PO
authors.lastname: Humbert
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D060049:Asymmetric Cell Division / Q000502:physiology*; D002352:Carrier Proteins / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000502:physiology*; D018797:Cell Cycle Proteins; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000378:metabolism; D016764:Cell Polarity; D002478:Cells, Cultured; D004900:Erythroblasts / Q000166:cytology* / Q000378:metabolism; D004920:Erythropoiesis / Q000502:physiology*; D005260:Female; D047908:Intracellular Signaling Peptides and Proteins / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D018797:Cell Cycle Proteins; D047908:Intracellular Signaling Peptides and Proteins; C502655:LGN protein, mouse; C115084:Pard3 protein, mouse; C508433:scrib protein, mouse