title: Human heart failure biomarker immunosensor based on excessively tilted fiber gratings.
abstract: A label-free immunosensor platform based on excessively tilted fiber gratings (Ex-TFGs) was developed for highly specific and fast detection of human N-terminal pro-B-type natriuretic peptide (NT-proBNP), which is considered a powerful biomarker for prognosis and risk stratification of heart failure (HF). High-purity anti-NT-proBNP monoclonal antibodies (MAbs) prepared in our laboratory were immobilized on fiber surface through the staphylococcal protein A (SPA) method for subsequent specific binding of the targeted NT-proBNP. Utilizing fiber optic grating demodulation system (FOGDS), immunoassays were carried outin vitroby monitoring the resonance wavelength shift of Ex-TFG biosensor with immobilized anti-NT-proBNP MAbs. Lowest detectable concentration of ~0.5ng/mL for NT-proBNP was obtained, and average sensitivity for NT-proBNP at a concentration range of 0~1.0 ng/mL was approximately 45.967 pm/(ng/mL). Several human serum samples were assessed by the proposed Ex-TFG biomarker sensor, with high specificity for NT-proBNP, indicating potential application in early diagnosing patients with acute HF symptoms.
authors.affiliation: Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, Chongqing University of Technology, Chongqing, 400054, China; School of Opto-electronic Information, University of Electronic Science and Technology of China, Chengdu, 610054, China; Aston Institute of Photonic Technologies, Aston University, Birmingham, B4 7ET, UK; luobinbin@cqut.edu.cn.
authors.forename: Binbin
authors.initials: B
authors.lastname: Luo
authors.affiliation: School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing, 400054, China.
authors.forename: Shengxi
authors.initials: S
authors.lastname: Wu
authors.affiliation: School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing, 400054, China.
authors.forename: Zhonghao
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, Chongqing University of Technology, Chongqing, 400054, China.
authors.forename: Wengen
authors.initials: W
authors.lastname: Zou
authors.affiliation: Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, Chongqing University of Technology, Chongqing, 400054, China.
authors.forename: Shenghui
authors.initials: S
authors.lastname: Shi
authors.affiliation: Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, Chongqing University of Technology, Chongqing, 400054, China.
authors.forename: Mingfu
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, Chongqing University of Technology, Chongqing, 400054, China.
authors.forename: Nianbing
authors.initials: N
authors.lastname: Zhong
authors.affiliation: School of Opto-electronic Information, University of Electronic Science and Technology of China, Chengdu, 610054, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, Chongqing University of Technology, Chongqing, 400054, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Zou
authors.affiliation: School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing, 400054, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Wang
authors.affiliation: The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400050, China.
authors.forename: Weina
authors.initials: W
authors.lastname: Chai
authors.affiliation: Department of Clinical Biochemistry, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Chuanmin
authors.initials: C
authors.lastname: Hu
authors.affiliation: Aston Institute of Photonic Technologies, Aston University, Birmingham, B4 7ET, UK.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
keywords: (060.2370) Fiber optics sensors; (170.0170) Medical optics and biotechnology; (280.1415) Biological sensing and sensors