title: Downregulation of Annexin A1 is correlated with radioresistance in nasopharyngeal carcinoma.
abstract: Radiotherapy is the primary treatment for nasopharyngeal carcinoma (NPC), but radioresistance often remains an obstacle to successful treatment. In our previous study, it was demonstrated that Annexin A1 (ANXA1) was involved in the p53-mediated radioresponse in NPC cells, which suggested that it may be associated with radioresistance in NPC; however, the role of ANXA1 in NPC radioresistance is unknown. In the present study, CNE2 cells were stably transfected with pLKO.1-ANXA1-small hairpin (sh)RNAs to investigate the effects of ANXA1 on the radiosensitivity of NPC. CNE2 cells transfected with pLKO.1 were used as the control. The radiosensitivities of the cellsin vitrowere analyzed using the clonogenic survival assay, cell growth analysis, flow cytometry and Hoechst 33258 staining. ANXA1 downregulation significantly enhanced clonogenic survival and cell growth following treatment of CNE2 cells with ionizing radiation (IR), increased the number of cells in the S phase and decreased IR-induced apoptosis. These results suggested that the radiosensitivity of CNE2 cells transfected with ANXA1-specific shRNA was significantly lower compared with the control cells. Therefore, ANXA1 downregulation may be involved in the radioresistance of NPC, and ANXA1 may be considered a novel biomarker for predicting NPC response to radiotherapy.
authors.affiliation: Institute of Nursing Research, School of Nursing, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Lifang
authors.initials: L
authors.lastname: Huang
authors.affiliation: Institute of Nursing Research, School of Nursing, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Liao
authors.affiliation: Institute of Nursing Research, School of Nursing, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wan
authors.affiliation: Cancer Research Institute, School of Medicine, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Ailan
authors.initials: A
authors.lastname: Cheng
authors.affiliation: Cancer Research Institute, School of Medicine, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Meixiang
authors.initials: M
authors.lastname: Li
authors.affiliation: Institute of Nursing Research, School of Nursing, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Sihan
authors.initials: S
authors.lastname: Chen
authors.affiliation: Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Maoyu
authors.initials: M
authors.lastname: Li
authors.affiliation: Institute of Nursing Research, School of Nursing, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Xing
authors.initials: X
authors.lastname: Tan
authors.affiliation: Institute of Nursing Research, School of Nursing, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Guqing
authors.initials: G
authors.lastname: Zeng
keywords: Annexin A1; ionizing radiation; nasopharyngeal carcinoma; radioresistance