title: 155R is a novel structural protein of bovine adenovirus type 3, but it is not essential for virus replication.
abstract: Bovine adenovirus (AdV) type 3 (BAdV-3) E1 region shares functional homology with E1 of human AdV type C5. Sequence analysis of the BAdV-3 E1 region revealed the presence of a novel 155R ORF that is not observed in other AdVs, on the lower strand antiparallel to a portion of the E1B region. The 155R gene products in BAdV-3-infected cells were identified by Northern blot, reverse transcriptase PCR followed by sequencing and Western blot analysis using the155R-specific antibody. 155R seems to be a late protein and is present in purified BAdV-3 particles. Replication kinetics of BAdV mutants with either one (BAdV/155R/mt1) or two (BAdV/155R/mt2) stop codons in the 155R ORF were comparable to those of BAdV-3, indicating that 155R is not essential for virus replication in cell culture. These results suggest that 155R-deleted BAdV-3 vectors could be generated in a cell line that fully complements BAdV-3 E1 functions.
authors.affiliation: Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.|Purdue Institute for Immunology, Inflammation and Infectious Diseases, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.|Purdue University Center for Cancer Research, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.
authors.forename: Ahmed O
authors.initials: AO
authors.lastname: Hassan
authors.affiliation: Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.|Purdue University Center for Cancer Research, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.|Present address: Merck Sharp and Dohme, West Point, PA, USA.
authors.forename: Sai V
authors.initials: SV
authors.lastname: Vemula
authors.affiliation: Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.|Purdue University Center for Cancer Research, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.|Present address: Department of Pediatrics, Weill Cornell Medical College, New York, NY, USA.
authors.forename: Anurag
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.|Purdue University Center for Cancer Research, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.|Present address: Department of Pathology, Sanofi Genzyme, 5 Mountain Road, Framingham, MA, USA.
authors.forename: Dinesh S
authors.initials: DS
authors.lastname: Bangari
authors.affiliation: Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.|Purdue University Center for Cancer Research, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.|Present address: Department of Biology, Ivy Tech Community College, Lafayette, IN, USA.
authors.forename: Krishna K
authors.initials: KK
authors.lastname: Mishra
authors.affiliation: Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.|Purdue Institute for Immunology, Inflammation and Infectious Diseases, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.|Purdue University Center for Cancer Research, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.
authors.forename: Suresh K
authors.initials: SK
authors.lastname: Mittal
mesh_terms: D000256:Adenoviridae / Q000235:genetics* / Q000502:physiology*; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002417:Cattle; D004252:DNA Mutational Analysis; D020869:Gene Expression Profiling; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D015678:Viral Structural Proteins / Q000235:genetics* / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D015678:Viral Structural Proteins