title: Solid supports for extraction and preconcentration of proteins and peptides in microfluidic devices: A review.
abstract: Determination of proteins and peptides is among the main challenges of today's bioanalytical chemistry. The application of microchip technology in this field is an exhaustively developed concept that aims to create integrated and fully automated analytical devices able to quantify or detect one or several proteins from a complex matrix. Selective extraction and preconcentration of targeted proteins and peptides especially from biological fluids is of the highest importance for a successful realization of these microsystems. Incorporation of solid structures or supports is a convenient solution employed to face these demands. This review presents a critical view on the latest achievements in sample processing techniques for protein determination using solid supports in microfluidics. The study covers the period from 2006 to 2015 and focuses mainly on the strategies based on microbeads, monolithic materials and membranes. Less common approaches are also briefly discussed. The reviewed literature suggests future trends which are discussed in the concluding remarks.
authors.affiliation: Univ. Paris-Est, ICMPE (UMR7182), CNRS, UPEC, Thiais 94320, France; Institut Galien Paris Sud, UMR8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Univ. Paris-Saclay, 5 rue Jean Baptiste Clément, 92290 Châtenay-Malabry, France; Medical University of Gdansk, Department of Toxicology, 107 Hallera Street, 80-416 Gdansk, Poland. Electronic address: dziomb@wp.pl.
authors.forename: Szymon
authors.initials: S
authors.lastname: Dziomba
authors.affiliation: Institut Galien Paris Sud, UMR8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Univ. Paris-Saclay, 5 rue Jean Baptiste Clément, 92290 Châtenay-Malabry, France. Electronic address: monica.araya-farias@u-psud.fr.
authors.forename: Monica
authors.initials: M
authors.lastname: Araya-Farias
authors.affiliation: Institut Galien Paris Sud, UMR8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Univ. Paris-Saclay, 5 rue Jean Baptiste Clément, 92290 Châtenay-Malabry, France. Electronic address: claire.smadja@u-psud.fr.
authors.forename: Claire
authors.initials: C
authors.lastname: Smadja
authors.affiliation: Institut Galien Paris Sud, UMR8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Univ. Paris-Saclay, 5 rue Jean Baptiste Clément, 92290 Châtenay-Malabry, France. Electronic address: myriam.taverna@u-psud.fr.
authors.forename: Myriam
authors.initials: M
authors.lastname: Taverna
authors.affiliation: Univ. Paris-Est, ICMPE (UMR7182), CNRS, UPEC, Thiais 94320, France. Electronic address: carbonnier@icmpe.cnrs.fr.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Carbonnier
authors.affiliation: Institut Galien Paris Sud, UMR8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Univ. Paris-Saclay, 5 rue Jean Baptiste Clément, 92290 Châtenay-Malabry, France. Electronic address: thuy.tran-maignan@u-psud.fr.
authors.forename: N Thuy
authors.initials: NT
authors.lastname: Tran
mesh_terms: D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D010455:Peptides / Q000032:analysis*; D011506:Proteins / Q000032:analysis*
chemical_list: D010455:Peptides; D011506:Proteins
keywords: Microchips; Microfluidics; Polymer monolith; Protein and peptide preconcentration; Solid support