title: Biosynthesis, Characterization, and Bioactivities Evaluation of Silver and Gold Nanoparticles Mediated by the Roots of Chinese Herbal Angelica pubescens Maxim.
abstract: A facile synthesis and biological applications of silver (DH-AgNps) and gold nanoparticles (DH-AuNps) mediated by the aqueous extract of Angelicae Pubescentis Radix (Du Huo) are explored. Du Huo is a medicinal root belonging to Angelica pubescens Maxim which possesses anti-inflammatory, analgesic, and antioxidant properties. The absorption spectra of nanoparticles in varying root extract and metal ion concentration, pH, reaction temperatures, and time were recorded by ultraviolet-visible (UV-Vis) spectroscopy. The presence of DH-AgNps and DH-AuNps was confirmed from the surface plasmon resonance intensified at ~414 and ~540 nm, respectively. Field emission transmission electron micrograph (FE-TEM) analysis revealed the formation of quasi-spherical DH-AgNps and spherical icosahedral DH-AuNps. These novel DH-AgNps and DH-AuNps maintained an average crystallite size of 12.48 and 7.44 nm, respectively. The biosynthesized DH-AgNps and DH-AuNps exhibited antioxidant activity against 2,2-diphenyl-1-picrylhydrzyl (DPPH) radicals and the former exhibited antimicrobial activity against clinical pathogens including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica. The expected presence of flavonoids, sesquiterpenes, and phenols on the nanoparticle surface were conjectured to grant protection against aggregation and free radical scavenging activity. DH-AgNps and DH-AuNps were further investigated for their cytotoxic properties in RAW264.7 macrophages for their potential application as drug carriers to sites of inflammation. In conclusion, this green synthesis is favorable for the advancement of plant mediated nano-carriers in drug delivery systems, cancer diagnostic, and medical imaging. Schematic diagram of biosynthesis of DH-AgNps and DH-AuNps and evaluation of their bioactivities.
authors.affiliation: Graduate School of Biotechnology and Ginseng Bank, College of Life Science, Kyung Hee University, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea.
authors.forename: Josua
authors.initials: J
authors.lastname: Markus
authors.affiliation: Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea.
authors.forename: Dandan
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea. yeonjukim@khu.ac.kr.
authors.forename: Yeon-Ju
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea.
authors.forename: Sungeun
authors.initials: S
authors.lastname: Ahn
authors.affiliation: Graduate School of Biotechnology and Ginseng Bank, College of Life Science, Kyung Hee University, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea.
authors.forename: Ramya
authors.initials: R
authors.lastname: Mathiyalagan
authors.affiliation: Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Graduate School of Biotechnology and Ginseng Bank, College of Life Science, Kyung Hee University, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea. dcyang@khu.ac.kr.|Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea. dcyang@khu.ac.kr.
authors.forename: Deok Chun
authors.initials: DC
authors.lastname: Yang
keywords: Angelica pubescens Maxim; Antimicrobial activity; Antioxidant activity; Cytotoxicity; Gold nanoparticles; RAW264.7; Silver nanoparticles