title: Quantitative assessment of hemodynamic and structural characteristics ofin vivobrain tissue using total diffuse reflectance spectrum measured in a non-contact fashion.
abstract: Here we present a new methodology that investigates the intrinsic structural and hemodynamic characteristics ofin vivobrain tissue, in a non-contact fashion, and can be easily incorporated in an intra-operative environment. Within this methodology, relative total diffuse reflectance spectra (RTD(λ)) were acquired from targets using a hybrid spectroscopy imaging system. A spectral interpretation algorithm was subsequently applied to RTD(λ) to retrieve optical properties related to the compositional and structural characteristics of each target. Estimation errors of the proposed methodology were computationally evaluated using a Monte Carlo simulation model for photon migration under various conditions. It was discovered that this new methodology could handle moderate noise and achieve very high accuracy, but only if the refractive index of the target is known. The accuracy of the technique was also validated using a series of tissue phantom studies, and consistent and accurate estimates of μs'(λ)/μa(λ) were obtained from all the phantoms tested. Finally, a small-scale animal study was conducted to demonstrate the clinical utility of the reported method, wherein a forepaw stimulation model was utilized to induce transient hemodynamic responses in somatosensory cortices. With this approach, significant stimulation-related changes (p < 0.001) in cortical hemodynamic and structural characteristics were successfully measured.
authors.affiliation: Department of Biomedical Engineering, College of Engineering and Computing, Florida International University, 10555 West Flagler St, EC 2610, Miami, FL 33174, USA.
authors.forename: Yinchen
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Biomedical Engineering, College of Engineering and Computing, Florida International University, 10555 West Flagler St, EC 2610, Miami, FL 33174, USA.
authors.forename: Sarahy
authors.initials: S
authors.lastname: Garcia
authors.affiliation: Department of Biomedical Engineering, College of Engineering and Computing, Florida International University, 10555 West Flagler St, EC 2610, Miami, FL 33174, USA.
authors.forename: Yisel
authors.initials: Y
authors.lastname: Frometa
authors.affiliation: Department of Biomedical Engineering, College of Engineering and Computing, Florida International University, 10555 West Flagler St, EC 2610, Miami, FL 33174, USA.
authors.forename: Jessica C
authors.initials: JC
authors.lastname: Ramella-Roman
authors.affiliation: Department of Biomedical Engineering, College of Engineering and Computing, Florida International University, 10555 West Flagler St, EC 2610, Miami, FL 33174, USA.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Soltani
authors.affiliation: Department of Biomedical Engineering, College of Engineering and Computing, Florida International University, 10555 West Flagler St, EC 2610, Miami, FL 33174, USA.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Almadi
authors.affiliation: Department of Biomedical Engineering, College of Engineering and Computing, Florida International University, 10555 West Flagler St, EC 2610, Miami, FL 33174, USA.
authors.forename: Jorge J
authors.initials: JJ
authors.lastname: Riera
authors.affiliation: Department of Biomedical Engineering, College of Engineering and Computing, Florida International University, 10555 West Flagler St, EC 2610, Miami, FL 33174, USA.
authors.forename: Wei-Chiang
authors.initials: WC
authors.lastname: Lin
keywords: (120.4640) Optical instruments; (170.3890) Medical optics instrumentation