title: Repair of a common bile duct defect with a decellularized ureteral graft.
abstract: OBJECTIVE
  To evaluate the feasibility of repairing a common bile duct defect with a decellularized ureteral graft in a porcine model.
  
  
  METHODS
  Eighteen pigs were randomly divided into three groups. An approximately 1 cm segment of the common bile duct was excised from all the pigs. The defect was repaired using a 2 cm long decellularized ureteral graft over a T-tube (T-tube group,n= 6) or a silicone stent (stent group,n= 6). Six pigs underwent bile duct reconstruction with a graft alone (stentless group). The surviving animals were euthanized at 3 mo. Specimens of the common bile ducts were obtained for histological analysis.
  
  
  RESULTS
  The animals in the T-tube and stent groups survived until sacrifice. The blood test results were normal in both groups. The histology results showed a biliary epithelial layer covering the neo-bile duct. In contrast, all the animals in the stentless group died due to biliary peritonitis and cholangitis within two months post-surgery. Neither biliary epithelial cells nor accessory glands were observed at the graft sites in the stentless group.
  
  
  CONCLUSIONS
  Repair of a common bile duct defect with a decellularized ureteral graft appears to be feasible. A T-tube or intraluminal stent was necessary to reduce postoperative complications.
authors.affiliation: Yao Cheng, Xian-Ze Xiong, Rong-Xing Zhou, Yi-Lei Deng, Yan-Wen Jin, Jiong Lu, Fu-Yu Li, Nan-Sheng Cheng, Department of Bile Duct Surgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Yao Cheng, Xian-Ze Xiong, Rong-Xing Zhou, Yi-Lei Deng, Yan-Wen Jin, Jiong Lu, Fu-Yu Li, Nan-Sheng Cheng, Department of Bile Duct Surgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
authors.forename: Xian-Ze
authors.initials: XZ
authors.lastname: Xiong
authors.affiliation: Yao Cheng, Xian-Ze Xiong, Rong-Xing Zhou, Yi-Lei Deng, Yan-Wen Jin, Jiong Lu, Fu-Yu Li, Nan-Sheng Cheng, Department of Bile Duct Surgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
authors.forename: Rong-Xing
authors.initials: RX
authors.lastname: Zhou
authors.affiliation: Yao Cheng, Xian-Ze Xiong, Rong-Xing Zhou, Yi-Lei Deng, Yan-Wen Jin, Jiong Lu, Fu-Yu Li, Nan-Sheng Cheng, Department of Bile Duct Surgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
authors.forename: Yi-Lei
authors.initials: YL
authors.lastname: Deng
authors.affiliation: Yao Cheng, Xian-Ze Xiong, Rong-Xing Zhou, Yi-Lei Deng, Yan-Wen Jin, Jiong Lu, Fu-Yu Li, Nan-Sheng Cheng, Department of Bile Duct Surgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
authors.forename: Yan-Wen
authors.initials: YW
authors.lastname: Jin
authors.affiliation: Yao Cheng, Xian-Ze Xiong, Rong-Xing Zhou, Yi-Lei Deng, Yan-Wen Jin, Jiong Lu, Fu-Yu Li, Nan-Sheng Cheng, Department of Bile Duct Surgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
authors.forename: Jiong
authors.initials: J
authors.lastname: Lu
authors.affiliation: Yao Cheng, Xian-Ze Xiong, Rong-Xing Zhou, Yi-Lei Deng, Yan-Wen Jin, Jiong Lu, Fu-Yu Li, Nan-Sheng Cheng, Department of Bile Duct Surgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
authors.forename: Fu-Yu
authors.initials: FY
authors.lastname: Li
authors.affiliation: Yao Cheng, Xian-Ze Xiong, Rong-Xing Zhou, Yi-Lei Deng, Yan-Wen Jin, Jiong Lu, Fu-Yu Li, Nan-Sheng Cheng, Department of Bile Duct Surgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
authors.forename: Nan-Sheng
authors.initials: NS
authors.lastname: Cheng
mesh_terms: D064591:Allografts / Q000637:transplantation; D000818:Animals; D001662:Biliary Tract Surgical Procedures / Q000379:methods*; D002763:Cholecystectomy / Q000009:adverse effects*; D003135:Common Bile Duct / Q000293:injuries / Q000601:surgery*; D004195:Disease Models, Animal; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D007049:Iatrogenic Disease; D008297:Male; D011183:Postoperative Complications / Q000517:prevention & control / Q000601:surgery*; D011897:Random Allocation; D019651:Plastic Surgery Procedures / Q000379:methods*; D015607:Stents; D034421:Sus scrofa; D014184:Transplantation, Homologous; D014513:Ureter / Q000637:transplantation*
keywords: Bile duct injury; Biliary reconstruction; Decellularization; Stent; Ureteral graft