title: Pericytes from Mesenchymal Stem Cells as a model for the blood-brain barrier.
abstract: Blood brain-barrier (BBB) in vitro models have been widely reported in studies of the BBB phenotype. However, established co-culture systems involve brain endothelial cells, astrocytes, neurons and pericytes, and therefore are often consuming and technically challenging. Here we use mesenchymal system cells (MSC) as a potential substitute for pericytes in a BBB model. Both MSC and pericyte markers in 2D culture environment were evaluated on different extracellular matrix compositions. Further experiments indicated that MSC contributed in a similar manner to pericytes in a co-cultured 3D model on increasing trans-endothelial electric resistance (TEER) and decreasing permeability against macromolecules.
authors.affiliation: School of Life Science, Anhui University, Hefei, China.|Department of Chemistry, University College London, London, UK.
authors.forename: Xiaohe
authors.initials: X
authors.lastname: Tian
authors.affiliation: School of Engineering and Materials Science, Queen Mary University of London, London, UK.
authors.forename: Oliver
authors.initials: O
authors.lastname: Brookes
authors.affiliation: Department of Chemistry, University College London, London, UK.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Battaglia
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods; D018920:Coculture Techniques / Q000379:methods; D005109:Extracellular Matrix; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D008810:Mice, Inbred C57BL; D008959:Models, Neurological; D020286:Pericytes / Q000166:cytology / Q000502:physiology*; D010539:Permeability