title: Brompheniramine as a novel probe for indirect UV detection and its application for the capillary electrophoresis of adamantane drugs.
abstract: Brompheniramine, an antihistamine drug, was employed as a novel UV probe for capillary electrophoresis with indirect UV detection of adamantane drugs (memantine, amantadine, and rimantadine). The probe possesses high molar absorptivity of 24 × 103L/mol cm at 6 mM, which enables the measurement of these nonchromophore analytes without derivatization. The simple background electrolyte (10 mM sodium dihydrogen phosphate (pH 5.0) containing 5 mM brompheniramine and 6 mM β-cyclodextrin) provided the separation of the analytes in a short time (7.5 min). Under these conditions, brompheniramine had similar mobility to that of the analyte ions resulting in symmetric peaks with minimal electrodispersion. The analytes displace the probe at a one-to-one ratio with transfer values close to unity. β-Cyclodextrin played a role in the resolution of the structurally similar adamantane derivatives. Method validation showed good linearity (r2> 0.98), precision (%RSD ≤ 3.30), and accuracy (recoveries ranging from 98 to 109%). The proposed method was successfully applied to determine the adamantane content in pharmaceutical products.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
authors.forename: Pornpan
authors.initials: P
authors.lastname: Prapatpong
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
authors.forename: Brompoj
authors.initials: B
authors.lastname: Prutthiwanasan
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
authors.forename: Nantana
authors.initials: N
authors.lastname: Nuchtavorn
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
authors.forename: Sawanya
authors.initials: S
authors.lastname: Buranaphalin
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
authors.forename: Leena
authors.initials: L
authors.lastname: Suntornsuk
mesh_terms: D000218:Adamantane / Q000032:analysis*; D001977:Brompheniramine / Q000737:chemistry*; D004573:Electrolytes; D019075:Electrophoresis, Capillary; D012299:Rimantadine / Q000032:analysis*
chemical_list: D004573:Electrolytes; D012299:Rimantadine; D001977:Brompheniramine; D000218:Adamantane
keywords: adamantane drugs; brompheniramine; capillary electrophoresis; cyclodextrin; indirect UV detection