title: Introduction: History of SH2 Domains and Their Applications.
abstract: The Src Homology 2 (SH2) domain is the prototypical protein interaction module that lies at the heart of phosphotyrosine signaling. Since its serendipitous discovery, there has been a tremendous advancement in technologies and an array of techniques available for studying SH2 domains and phosphotyrosine signaling. In this chapter, we provide a glimpse of the history of SH2 domains and describe many of the tools and techniques that have been developed along the way and discuss future directions for SH2 domain studies. We highlight the gist of each chapter in this volume in the context of: the structural biology and phosphotyrosine binding; characterizing SH2 specificity and generating prediction models; systems biology and proteomics; SH2 domains in signal transduction; and SH2 domains in disease, diagnostics, and therapeutics. Many of the individual chapters provide an in-depth approach that will allow scientists to interrogate the function and role of SH2 domains.
authors.affiliation: Broad Institute of Harvard and MIT, 415 Main St., 5175 JJ, Cambridge, MA, 02142, USA. baliu@broadinstitute.org.
authors.forename: Bernard A
authors.initials: BA
authors.lastname: Liu
authors.affiliation: Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Genome Sciences, University of Connecticut School of Medicine, 400 Farmington Ave., Farmington, CT, 06030, USA. machida@uchc.edu.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Machida
mesh_terms: D000818:Animals; D004198:Disease Susceptibility; D055808:Drug Discovery; D006801:Humans; D008958:Models, Molecular; D025202:Molecular Diagnostic Techniques; D010766:Phosphorylation; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping / Q000379:methods; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D012106:Research; D015398:Signal Transduction; D013329:Structure-Activity Relationship; D049490:Systems Biology / Q000379:methods; D018909:src Homology Domains
chemical_list: D011506:Proteins
keywords: High-throughput screening; Phosphoproteomics; SH2 binding assays; SH2 domain; SH2 domain discovery; SH2 specificity; SPOT; pTyr peptides