title: EcoTILLING by sequencing reveals polymorphisms in genes encoding starch synthases that are associated with low glycemic response in rice.
abstract: Glycemic response, a trait that is tedious to be assayed in cereal staples, has been identified as a factor correlated with alarmingly increasing prevalence of Type II diabetes. Reverse genetics based discovery of allelic variants associated with this nutritional trait gains significance as they can provide scope for genetic improvement of this factor which is otherwise difficult to target through routine screening methods.
  
  
  
  Through EcoTILLING by sequencing in 512 rice accessions, we report the discovery of six deleterious variants in the genes with potential to increase Resistant Starch (RS) and reduce Hydrolysis Index (HI) of starch. By deconvolution of the variant harbouring EcoTILLING DNA pools, we discovered accessions with a minimum of one to a maximum of three deleterious allelic variants in the candidate genes.
  
  
  
  Through biochemical assays, we confirmed the potential role of the discovered alleles alone or in combinations in increasing RS the key factor for reduction in glycemic response.
authors.affiliation: Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, 641 003, Tamil Nadu, India.|Department of Plant Sciences, University of Saskatchewan, Saskatoon, SK, S7N 5A8, Canada.
authors.forename: Ramadoss Bharathi
authors.initials: RB
authors.lastname: Raja
authors.affiliation: Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, 641 003, Tamil Nadu, India.
authors.forename: Somanath
authors.initials: S
authors.lastname: Agasimani
authors.affiliation: Department of Plant Sciences, University of Saskatchewan, Saskatoon, SK, S7N 5A8, Canada.
authors.forename: Sarita
authors.initials: S
authors.lastname: Jaiswal
authors.affiliation: Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, 641 003, Tamil Nadu, India.
authors.forename: Venkatesan
authors.initials: V
authors.lastname: Thiruvengadam
authors.affiliation: Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, 641 003, Tamil Nadu, India.
authors.forename: Robin
authors.initials: R
authors.lastname: Sabariappan
authors.affiliation: Department of Plant Sciences, University of Saskatchewan, Saskatoon, SK, S7N 5A8, Canada.
authors.forename: Ravindra N
authors.initials: RN
authors.lastname: Chibbar
authors.affiliation: Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, 641 003, Tamil Nadu, India. ganeshgene@gmail.com.
authors.forename: Sundaram Ganesh
authors.initials: SG
authors.lastname: Ram
mesh_terms: D000483:Alleles; D009154:Mutation; D012275:Oryza / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013213:Starch / Q000378:metabolism*; D013214:Starch Synthase / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D013213:Starch; D013214:Starch Synthase
keywords: Allele mining; EcoTILLING by sequencing; Glycemic response; Resistant starch; Rice; Starch biosynthesis