title: [Establishment of a SD rat model of vulvar lichen simplex chronicus and detection of the expression of protease activated receptor 2].
abstract: OBJECTIVE
  To establish a SD rat model of vulvar lichen simplex chronicus (LSC) and investigate the expression of protease activated receptor 2 (PAR2) in the genital skin.
  
  
  METHODS
  Seventy female SD rats were randomly divided into group A (blank control group, n=10), group B (with application of acetone solution 3 times per week for 10 weeks, n=10), group C (with chronic mechanical irritation 3 times per week for 10 weeks, n=10), and group D (with topical treatment with 0.5= 7,12-Dimethylbenzanthracene [DMBA] in acetone solution and chronic mechanical irritation 3 times per week for 10 weeks, n=40). The changes of the genital skin changes were observed regularly and the expression of PAR2 in groups A and D was detected with immunohistochemistry, Western blotting and qRT-PCR.
  
  
  RESULTS
  In group D, LSC occurred in 23 rats (57.5=) at 8 weeks and in 38 rats (95=) at 10 weeks; 8 rats (20=) showed papilloma at 12 weeks. Acetone treatment or chronic mechanical irritation did not cause LSC in the rats. Immunohistochemistry, Western blotting and qRT-PCR showed significantly increased expressions of PAR2 in group D at both the protein and mRNA levels as compared with those in group A (P<0.05).
  
  
  CONCLUSIONS
  0.5= DMBA in acetone solution along with chronic mechanical irritation can induce LSC in female SD rats, and PAR2 is closely related with the occurrence and progression of LSC.
authors.affiliation: State Key Laboratory of Ultrasound Engineering in Medicine Co-Founded by Chongqing and the Ministry of Science and Technology, College of Biomedical Engineering, Chongqing Key Laboratory of Biomedical Engineering, Chongqing Collaborative Innovation Center for Minimally-invasive and Noninvasive Medicine; Chongqing Medical University, Chongqing 40016, China.E-mail: yanghuan1822@foxmail.com.
authors.forename: Huan
authors.initials: H
authors.lastname: Yang
authors.forename: Zhen-Hua
authors.initials: ZH
authors.lastname: Fu
authors.forename: Hua-Jun
authors.initials: HJ
authors.lastname: Tang
authors.forename: Cheng-Zhi
authors.initials: CZ
authors.lastname: Li
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene; D000096:Acetone; D000818:Animals; D002273:Carcinogens; D004195:Disease Models, Animal; D005260:Female; D017276:Friction; D009450:Neurodermatitis / Q000139:chemically induced / Q000378:metabolism*; D010212:Papilloma / Q000209:etiology; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D044464:Receptor, PAR-2 / Q000235:genetics / Q000378:metabolism*; D012997:Solvents; D014845:Vulvar Diseases / Q000139:chemically induced / Q000378:metabolism*; D014846:Vulvar Neoplasms / Q000209:etiology
chemical_list: D002273:Carcinogens; D012333:RNA, Messenger; D044464:Receptor, PAR-2; D012997:Solvents; D000096:Acetone; D015127:9,10-Dimethyl-1,2-benzanthracene