title: Development of a quantum dot-based immunochromatography test strip for rapid screening of oxytetracycline and 4-epi-oxytetracycline in edible animal tissues.
abstract: A rapid and sensitive immunochromatographic test strip (ICTS) was developed in a competitive format with the quantum dot-conjugated monoclonal antibody specifically to determine the residues of oxytetracycline (OTC) and its metabolite 4-epi-oxytetracycline (4-epi-OTC). Using an ICTS reader, the 50% inhibition concentration (IC50) was found to be 3.41 ± 0.29 ng ml-1of OTC in phosphate-buffered saline samples and the detection limit was 0.44 ± 0.05 ng ml-1. The visual cut-off level of the ICTS is 25 ng ml-1. The recoveries from tissue samples spiked with OTC of 50-600 μg kg-1were 74.2-107.2%, with coefficients of variation below 15%. The method was used to analyse incurred tissue samples, and there was good correlation (R2= 0.999) between the ICTS and high-performance liquid chromatography. These results indicate that ICTS is suitable for the rapid and quantitative detection of OTC and 4-epi-OTC residues in edible animal tissues.
authors.affiliation: a College of Life Science , Chongqing Normal University , Chongqing , China.|b Department of Animation , Chongqing College of Electronic Engineering , Chongqing , China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Xie
authors.affiliation: a College of Life Science , Chongqing Normal University , Chongqing , China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: a College of Life Science , Chongqing Normal University , Chongqing , China.
authors.forename: Xian
authors.initials: X
authors.lastname: Yang
authors.affiliation: a College of Life Science , Chongqing Normal University , Chongqing , China.
authors.forename: Tao
authors.initials: T
authors.lastname: Le
mesh_terms: D000818:Animals; D002645:Chickens; D002846:Chromatography, Affinity / Q000295:instrumentation*; D002851:Chromatography, High Pressure Liquid; D005504:Food Analysis / Q000295:instrumentation*; D010118:Oxytetracycline / Q000031:analogs & derivatives* / Q000032:analysis*; D010316:Particle Size; D045663:Quantum Dots; D012756:Sheep; D013237:Stereoisomerism; D013499:Surface Properties; D013552:Swine
chemical_list: D010118:Oxytetracycline
keywords: Oxytetracycline; immunochromatography test strip (ICTS); quantitative detection; quantum dot