title: Structure of O-specific polysaccharide of Oligotropha carboxidovorans OM5 - a wastewater bacterium.
abstract: Oligotropha carboxidovorans strain OM5 (previously known as Pseudomonas carboxydovorans OM5) is a rod-shaped Gram-negative bacterium isolated from wastewater. This bacterium is able to live in aerobic and, facultatively, in autotrophic conditions. For autotrophic growth, the bacteria can utilize carbon monoxide or hydrogen as a source of energy. The O-specific polysaccharide isolated from O. carboxidovorans OM5 lipopolysaccharide was structurally characterized using chemical analyses, 1D and 2D NMR spectroscopy, and MALDI-TOF mass spectrometry techniques. The polysaccharide was found to be a homopolymer built up of 3-O-methyl-α-d-mannose residues linked by (1 → 2)-glycosidic bonds. The degree of polymerization of high-molecular-weight polysaccharide was estimated at approximately 35-40 units. The structure of the homopolymer is depicted below: [Formula: see text].
authors.affiliation: Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Lublin, Poland. Electronic address: iwona.komaniecka@poczta.umcs.lublin.pl.
authors.forename: Iwona
authors.initials: I
authors.lastname: Komaniecka
authors.affiliation: Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Lublin, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Choma
authors.affiliation: Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Lublin, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Zamlynska
authors.affiliation: Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Lublin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Sroka-Bartnicka
authors.affiliation: Intercollegiate NMR Laboratory, Department of Chemistry, Gdansk University of Technology, Poland.
authors.forename: Pawel
authors.initials: P
authors.lastname: Sowinski
mesh_terms: D000332:Aerobiosis; D052818:Autotrophic Processes; D041841:Bradyrhizobiaceae / Q000737:chemistry* / Q000378:metabolism; D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000031:analogs & derivatives*; D008970:Molecular Weight; D058105:Polymerization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D062065:Wastewater / Q000382:microbiology; D014871:Water Microbiology
chemical_list: D008070:Lipopolysaccharides; D008358:Mannose; D062065:Wastewater
keywords: 3-O-methylmannose; Lipopolysaccharide; O-specific polysaccharide; Oligotropha carboxidovorans