title: Mother gestational exposure to organophosphorus pesticide induces neuron and glia loss in daughter adult brain.
abstract: Chlorpyrifos (CPF) is a widely used organophosphorus pesticide with developmental neurotoxicity such as morphogenesis toxicity. In the present study, we assessed the effects of prenatal CPF exposure on systemic parameters and cytoarchitecture of medial prefrontal cortex (mPFC) in adulthood. Gestational dams were exposed to 5mg/kg/d of CPF during gestational days 13-17, while body weight, organ coefficient, and neuron and glia counts of offspring were determined on postnatal day 60. Our results showed that CPF treatment induced little or no effects on body weight and organ coefficients. There were also no significant pathological changes in mPFC. However, neuron and glia count analysis showed that CPF treatment reduced neuron and glia counts in anterior cingulate, prelimbic, and infralimbic areas of mPFC. The CPF react pattern was similar in both sexes, and there was no statistical difference in most of the sub-regions. Thus, our results revealed an embryonic origin brain deficit induced by gestational mother pesticide exposure.
authors.affiliation: a College of Biological and Environmental Engineering, Zhejiang University of Technology , Hangzhou , China.
authors.forename: Xiao P
authors.initials: XP
authors.lastname: Chen
authors.affiliation: a College of Biological and Environmental Engineering, Zhejiang University of Technology , Hangzhou , China.
authors.forename: Yong S
authors.initials: YS
authors.lastname: Chao
authors.affiliation: a College of Biological and Environmental Engineering, Zhejiang University of Technology , Hangzhou , China.
authors.forename: Wen Z
authors.initials: WZ
authors.lastname: Chen
authors.affiliation: b School of Medicine and Life Sciences, Zhejiang University City College , Hangzhou , China.
authors.forename: Jing Y
authors.initials: JY
authors.lastname: Dong
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000473:pathology*; D004390:Chlorpyrifos / Q000633:toxicity*; D005260:Female; D008297:Male; D018811:Maternal Exposure / Q000009:adverse effects; D008813:Mice, Inbred ICR; D009457:Neuroglia / Q000187:drug effects / Q000473:pathology; D009474:Neurons / Q000187:drug effects* / Q000473:pathology; D010575:Pesticides / Q000633:toxicity*; D017397:Prefrontal Cortex / Q000187:drug effects / Q000473:pathology; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000473:pathology*
chemical_list: D010575:Pesticides; D004390:Chlorpyrifos
keywords: Organophosphorus pesticide; daughter adulthood brain; gestational mother exposure; glia count; medial prefrontal cortex; neuron count