title: Multi-response optimization of Artemia hatching process using split-split-plot design based response surface methodology.
abstract: A novel method, BBD-SSPD is proposed by the combination of Box-Behnken Design (BBD) and Split-Split Plot Design (SSPD) which would ensure minimum number of experimental runs, leading to economical utilization in multi- factorial experiments. The brine shrimp Artemia was tested to study the combined effects of photoperiod, temperature and salinity, each with three levels, on the hatching percentage and hatching time of their cysts. The BBD was employed to select 13 treatment combinations out of the 27 possible combinations that were grouped in an SSPD arrangement. Multiple responses were optimized simultaneously using Derringer's desirability function. Photoperiod and temperature as well as temperature-salinity interaction were found to significantly affect the hatching percentage of Artemia, while the hatching time was significantly influenced by photoperiod and temperature, and their interaction. The optimum conditions were 23 h photoperiod, 29 °C temperature and 28 ppt salinity resulting in 96.8% hatching in 18.94 h. In order to verify the results obtained from BBD-SSPD experiment, the experiment was repeated preserving the same set up. Results of verification experiment were found to be similar to experiment originally conducted. It is expected that this method would be suitable to optimize the hatching process of animal eggs.
authors.affiliation: ICAR-Central Institute of Fisheries Education, Mumbai, India.
authors.forename: V V
authors.initials: VV
authors.lastname: Arun
authors.affiliation: ICAR-Central Institute of Fisheries Education, Mumbai, India.
authors.forename: Neelam
authors.initials: N
authors.lastname: Saharan
authors.affiliation: ICAR-Central Institute of Fisheries Education, Mumbai, India.
authors.forename: V
authors.initials: V
authors.lastname: Ramasubramanian
authors.affiliation: ICAR-Central Institute of Fisheries Education, Mumbai, India.
authors.forename: A M
authors.initials: AM
authors.lastname: Babitha Rani
authors.affiliation: Aquaculture and Aquatic Resources Management, Asian Institute of Technology, Thailand.
authors.forename: K R
authors.initials: KR
authors.lastname: Salin
authors.affiliation: ICAR-Central Institute of Fisheries Education, Mumbai, India.
authors.forename: Ravindra
authors.initials: R
authors.lastname: Sontakke
authors.affiliation: ICAR-Central Institute of Fisheries Education, Mumbai, India.
authors.forename: Harsha
authors.initials: H
authors.lastname: Haridas
authors.affiliation: Department of Primary Industry and Fisheries, Northern Territory Government, Australia.
authors.forename: Deepak George
authors.initials: DG
authors.lastname: Pazhayamadom
mesh_terms: D000818:Animals; D001156:Artemia / Q000254:growth & development*; D008954:Models, Biological; D017440:Photoperiod; D010827:Physiology / Q000379:methods*; D054712:Salinity; D013696:Temperature; D013997:Time Factors