title: Transcriptomic Analysis of Laribacter hongkongensis Reveals Adaptive Response Coupled with Temperature.
abstract: Bacterial adaptation to different hosts requires transcriptomic alteration in response to the environmental conditions. Laribacter hongkongensis is a gram-negative, facultative anaerobic, urease-positive bacillus caused infections in liver cirrhosis patients and community-acquired gastroenteritis. It was also found in intestine from commonly consumed freshwater fishes and drinking water reservoirs. Since L. hongkongensis could survive as either fish or human pathogens, their survival mechanisms in two different habitats should be temperature-regulated and highly complex. Therefore, we performed transcriptomic analysis of L. hongkongensis at body temperatures of fish and human in order to elucidate the versatile adaptation mechanisms coupled with the temperatures. We identified numerous novel temperature-induced pathways involved in host pathogenesis, in addition to the shift of metabolic equilibriums and overexpression of stress-related proteins. Moreover, these pathways form a network that can be activated at a particular temperature, and change the physiology of the bacteria to adapt to the environments. In summary, the dynamic of transcriptomes in L. hongkongensis provides versatile strategies for the bacterial survival at different habitats and this alteration prepares the bacterium for the challenge of host immunity.
authors.affiliation: Department of Biomedical Sciences, City University of Hong Kong, Kowloon, Hong Kong Special Administrative Region, China.
authors.forename: Hoi-Kuan
authors.initials: HK
authors.lastname: Kong
authors.affiliation: Department of Biomedical Sciences, City University of Hong Kong, Kowloon, Hong Kong Special Administrative Region, China.
authors.forename: Hon-Wai
authors.initials: HW
authors.lastname: Law
authors.affiliation: Department of Biomedical Sciences, City University of Hong Kong, Kowloon, Hong Kong Special Administrative Region, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Biomedical Sciences, City University of Hong Kong, Kowloon, Hong Kong Special Administrative Region, China.
authors.forename: Carmen O K
authors.initials: CO
authors.lastname: Law
authors.affiliation: Department of Biomedical Sciences, City University of Hong Kong, Kowloon, Hong Kong Special Administrative Region, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Pan
authors.affiliation: Department of Biomedical Sciences, City University of Hong Kong, Kowloon, Hong Kong Special Administrative Region, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of Microbiology and Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Xiong
authors.affiliation: Department of Microbiology and Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.
authors.forename: Susanna K P
authors.initials: SK
authors.lastname: Lau
authors.affiliation: Department of Microbiology and Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.
authors.forename: Patrick C Y
authors.initials: PC
authors.lastname: Woo
authors.affiliation: Department of Biomedical Sciences, City University of Hong Kong, Kowloon, Hong Kong Special Administrative Region, China.
authors.forename: Terrence Chi Kong
authors.initials: TC
authors.lastname: Lau
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D015415:Biomarkers / Q000378:metabolism*; D005759:Gastroenteritis / Q000235:genetics* / Q000382:microbiology; D016680:Genome, Bacterial; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D009346:Neisseriaceae / Q000235:genetics* / Q000302:isolation & purification; D012329:RNA, Bacterial / Q000235:genetics; D013312:Stress, Physiological; D013696:Temperature
chemical_list: D015415:Biomarkers; D012329:RNA, Bacterial