title: Purification and biochemical characterization of a novel thermostable serine alkaline protease from Aeribacillus pallidus C10: a potential additive for detergents.
abstract: An extracellular thermostable alkaline serine protease enzyme from Aeribacillus pallidus C10 (GenBank No: KC333049), was purified 4.85 and 17. 32-fold with a yield of 26.9 and 19.56%, respectively, through DE52 anion exchange and Probond affinity chromatography. The molecular mass of the enzyme was determined through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), with approximately 38.35 kDa. The enzyme exhibited optimum activity at pH 9 and at temperature 60 °C. It was determined that the enzyme had remained stable at the range of pH 7.0-10.0, and that it had preserved more than 80% of its activity at a broad temperature range (20-80 °C). The enzyme activity was found to retain more than 70% and 55% in the presence of organic solvents and commercial detergents, respectively. In addition, it was observed that the enzyme activity had increased in the presence of 5% SDS. KMand Vmaxvalues were calculated as 0.197 mg/mL and 7.29 μmol.mL-1.min-1, respectively.
authors.affiliation: a Department of Molecular Biology and Genetics, Faculty of Science , Ataturk University , Erzurum , Turkey.
authors.forename: Vildan
authors.initials: V
authors.lastname: Yildirim
authors.affiliation: a Department of Molecular Biology and Genetics, Faculty of Science , Ataturk University , Erzurum , Turkey.
authors.forename: Mustafa Ozkan
authors.initials: MO
authors.lastname: Baltaci
authors.affiliation: b Department of Chemistry, Faculty of Science , Ataturk University , Erzurum , Turkey.
authors.forename: Ilknur
authors.initials: I
authors.lastname: Ozgencli
authors.affiliation: a Department of Molecular Biology and Genetics, Faculty of Science , Ataturk University , Erzurum , Turkey.
authors.forename: Melda
authors.initials: M
authors.lastname: Sisecioglu
authors.affiliation: a Department of Molecular Biology and Genetics, Faculty of Science , Ataturk University , Erzurum , Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Adiguzel
authors.affiliation: c Department of Food Hygiene and Technology, Faculty of Veterinary , Ataturk University , Erzurum , Turkey.
authors.forename: Gulsah
authors.initials: G
authors.lastname: Adiguzel
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D003902:Detergents / Q000737:chemistry*; D010450:Endopeptidases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012694:Serine / Q000737:chemistry*; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D003902:Detergents; D012694:Serine; D010450:Endopeptidases; C087822:alkaline protease
keywords: Aeribacillus pallidus; Thermotolerant; alkaline serine protease; biochemical characterization; purification