title: MicroRNA-297a regulates vascular calcification by targeting fibroblast growth factor 23.
abstract: OBJECTIVE
  Vascular calcification is one the major characteristics in patients with various types of chronic inflammatory disorders. MiRNAs have been shown to be involved in many normal biological functions as well as diseases; however, their role in vascular calcification has not received much attention.
  
  
  METHODS
  In the current study, we built a vascular calcification rat model using vitamin D3 plus nicotine and analyzed miRNA expression profile by miRNA chip assay. Potential target of one selected miRNA with sharpest variation in expression were predicted by both PicTar and TargetScan. The impact of the selected miRNA on the expression of the potential target on both mRNA and protein levels were measured by RT-PCR and Western blot, respectively.
  
  
  RESULTS
  Our results identified 16 dysregulated miRNAs, among which miR-297a showed the sharpest variation. Further analysis focusing on miR-297a revealed that fibroblast growth factor 23 (FGF23) was a potential target of miR297a. Measurement of FGF23 and its regulator Klotho on both mRNA and protein levels demonstrated that FGF23 was significantly increased while Klotho was decreased in rats with vascular calcification.
  
  
  CONCLUSIONS
  Our results indicated that FGF23 was target of miR-297a and decreased miR-297a in vascular calcification lead to the increase of FGF23, which together with Klotho might enhance vascular calcification. The findings of this study could provide valuable information for the understanding of mechanisms underlying miR-dependent vascular calcification as well as potential treatment target for the disease.
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052 China.
authors.forename: Shouhua
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052 China.
authors.forename: Shuijun
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052 China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052 China.
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Guo
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052 China.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Zhai
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052 China.
authors.forename: Xinguang
authors.initials: X
authors.lastname: Qiu
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052 China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li
keywords: Chronic inflammatory disorder; Fibroblast growth factor 23; Klotho; MicroRNA-297a; Vascular calcification