title: Effects of Ambient Fine Particles PM2.5on Human HaCaT Cells.
abstract: The current study was conducted to observe the effects of fine particulate matter (PM2.5) on human keratinocyte cell line (HaCaT) cells. The potential mechanism linking PM2.5and skin was explored. HaCaT cells were cultured and then accessed in plate with PM2.5. Cell viability was tested by Cell Counting Kit-8. The mRNA and protein expression of Filaggrin, Loricrin, Involucrin, and Repetin were analyzed. The levels of Granulocyte-macrophage Colony Stimulating Factor, Thymic Stromal Lymphopoietin, Tumor Necrosis Factor-α, Interleukin-1α, and Interleukin-8 were detected in the supernatant of the HaCaT cell with enzyme-linked immunosorbent assay kits. Cell viability decreased with the increase in PM2.5. Compared with the control group, the protein expression of Filaggrin, Repetin, Involucrin, and Loricrin showed different expression patterns in PM2.5treatment groups. The level of Tumor Necrosis Factor-α, Thymic Stromal Lymphopoietin, Interleukin-1α, and Interleukin-8 significantly increased in the cells treated with PM2.5. Ambient PM2.5may increase the risk of eczema and other skin diseases. The relative mechanism may be associated with the impairment of the skin barrier and the elevation of inflammatory responses.
authors.affiliation: Department of Dermatology, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai 200040, China. dr_liqiao@163.com.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai 200040, China. kangban@163.com.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Kang
authors.affiliation: Department of Environmental Health, School of Public Health and the Key Laboratory of Public Health Safety, Ministry of Education, Fudan University, Shanghai 200032, China. jiangshuofd@sina.com.
authors.forename: Shuo
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Department of Environmental Health, School of Public Health and the Key Laboratory of Public Health Safety, Ministry of Education, Fudan University, Shanghai 200032, China. Zhaojinzhuofd@sina.com.
authors.forename: Jinzhuo
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Dermatology, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai 200040, China. yanshuxianhs@sina.com.
authors.forename: Shuxian
authors.initials: S
authors.lastname: Yan
authors.affiliation: Department of Dermatology, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai 200040, China. xufeng@medmail.com.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.affiliation: Department of Dermatology, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai 200040, China. xujinhua2012@sina.com.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Xu
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D000091344:Filaggrin Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000187:drug effects; D006801:Humans; D053582:Interleukin-1alpha / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D015603:Keratinocytes / Q000187:drug effects*; D052638:Particulate Matter / Q000494:pharmacology*; D011498:Protein Precursors; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D016207:Cytokines; C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D053582:Interleukin-1alpha; D016209:Interleukin-8; D052638:Particulate Matter; D011498:Protein Precursors; D014409:Tumor Necrosis Factor-alpha; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D000094632:Thymic Stromal Lymphopoietin
keywords: HaCaT cell; PM2.5; skin damage