title: Down-regulation of a chitin synthase a gene by RNA interference enhances pathogenicity of Beauveria bassiana ANU1 against Spodoptera exigua (HÜBNER).
abstract: Chitin synthase (CHS) is an important enzymatic component, which is required for chitin formation in the cuticles and cuticular linings of other tissues in insects. CHSs have been divided into two classes, classes A and B, based on their amino acid sequence similarities and functions. Class A CHS (CHS-A) is specifically expressed in the epidermis and related ectodermal cells such as tracheal cells, while class B CHS (CHS-B) is expressed in gut epithelial cells that produce peritrophic matrices. In this study, we cloned the CHS-A gene from the beet armyworm, Spodoptera exigua (SeCHS-A). The SeCHS-A contains an open reading frame of 4,698 nucleotides, encoding a protein of 1,565 amino acids with a predicted molecular mass of approximately 177.8 kDa. The SeCHS-A mRNA was expressed in all developmental stages and specifically in the epidermis and tracheae tissue by quantitative real-time-PCR analysis. Expression of SeCHS-A gene was suppressed by feeding double-stranded RNA (dsCHS-A, 400 ng/larva) in the third instar larvae of S. exigua. Suppression of the SeCHS-A gene expression significantly increased 35% of mortality on pupation of S. exigua. Also, the third instar larvae fed with dsCHS-A significantly increased susceptibility to entomopathogenic fungi, Beauveria bassiana ANU1 at 3 days after treatment. These results suggest that the SeCHS-A gene plays an important role in development of S. exigua and RNA interference may apply to effective pest control with B. bassiana.
authors.affiliation: Department of Bioresource Sciences, Andong National University, Andong, Republic of Korea.
authors.forename: Jung-Bok
authors.initials: JB
authors.lastname: Lee
authors.affiliation: Department of Plant Medicine, Andong National University, Andong, Republic of Korea.
authors.forename: Hyun Soo
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Bioresource Sciences, Andong National University, Andong, Republic of Korea.
authors.forename: Youngjin
authors.initials: Y
authors.lastname: Park
mesh_terms: D000818:Animals; D052982:Beauveria / Q000472:pathogenicity*; D001681:Biological Assay; D061046:Biological Control Agents; D002687:Chitin Synthase / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation; D007814:Larva / Q000201:enzymology / Q000235:genetics / Q000382:microbiology; D034622:RNA Interference; D018411:Spodoptera / Q000201:enzymology* / Q000235:genetics / Q000382:microbiology*
chemical_list: D061046:Biological Control Agents; D002687:Chitin Synthase
keywords: Beauveria bassiana; RNA interference; Spodoptera exigua; biopesticide; chitin synthase