title: One-step synthesis of DNA functionalized cadmium-free quantum dots and its application in FRET-based protein sensing.
abstract: DNA functionalized quantum dots (QDs) are promising nanoprobes for the fluorescence resonance energy transfer (FRET)-based biosensing. Herein, cadmium-free DNA functionalized Mn-doped ZnS (DNA-ZnS:Mn2+) QDs were successfully synthesized by one-step route. As-synthesized QDs show excellent photo-stability with the help of PAA and DNA. Then, we constructed a novel FRET model based on the QDs and WS2nanosheets as the energy donor-acceptor pairs, which was successfully applied for the protein detection through the terminal protection of small molecule-linked DNA assay. This work not only explores the potential bioapplication of the DNA-ZnS:Mn2+QDs, but also provides a platform for the investigation of small molecule-protein interaction.
authors.affiliation: Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China. Electronic address: clzhang@chem.ecnu.edu.cn.
authors.forename: Cuiling
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China.
authors.forename: Caiping
authors.initials: C
authors.lastname: Ding
authors.affiliation: School of Chemistry and Chemical Engineering, Lingnan Normal University, Zhanjiang, 524048, China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Zhou
authors.affiliation: Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Xue
authors.affiliation: Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China. Electronic address: yzxian@chem.ecnu.edu.cn.
authors.forename: Yuezhong
authors.initials: Y
authors.lastname: Xian
mesh_terms: D002104:Cadmium; D004247:DNA; D031541:Fluorescence Resonance Energy Transfer; D017895:Manganese Compounds; D011506:Proteins / Q000032:analysis*; D045663:Quantum Dots; D013440:Sulfides; D017967:Zinc Compounds
chemical_list: D017895:Manganese Compounds; D011506:Proteins; D013440:Sulfides; D017967:Zinc Compounds; D002104:Cadmium; D004247:DNA
keywords: Biosensor; FRET; Quantum dots; Terminal protection of small-molecule linked DNA; WS(2)