title: Effects of nano tantalum implants on inducing osteoblast proliferation and differentiation.
abstract: In the present study, we examined the effects of nano tantalum (Ta) dental implants on inducing osteoblast proliferation and differentiation. The MG-63 osteoblasts were divided into 3 groups after recovery, passage and storage: i) Osteoblast culturing group (control group); ii) osteoblast and titanium (Ti) implant co-culturing group (Ti group); and iii) osteoblast and Ta implant co-culturing group (Ta group). After 7 days, a scanning electron microscope was used to observe the growth status, number and morphological changes of the cells on the surfaces of the materials. An MTT assay was used to detect cell proliferation after culturing for 1, 3 and 7 days. ELISA assay was used to detect the levels of alkaline phosphatase (ALP) after 1, 3 and 7 days. Western blot analysis was used to detect the expression levels of collagen type I (Col-1) and osteocalcin after 1, 3 and 7 days. There was significant cell spreading on the surfaces of Ti and of Ta after 7 days, flat and with many pseudopodia. Additionally, there were more cell components in the Ta group. Concurrently, cell proliferation in the Ti and Ta groups increased. There was also an increase in the level of ALP and the expression level of Col-1 over time. The indexes of the Ta group were more apparent than those of the Ti group at each time-point, and the differences were statistically significant (p<0.05). In conclusion, compared with Ti implants, Ta implants induced more osteoblast proliferation and differentiation.
authors.affiliation: Stomatological College of Shandong University, Jinan, Shandong 250012, P.R. China; Shandong Province Key Laboratory of Oral Biology Medicine, Jinan, Shandong 250012, P.R. China; Department of Stomatology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Stomatology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of Orthopedics, The Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250012, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Stomatological College of Shandong University, Jinan, Shandong 250012, P.R. China; Shandong Province Key Laboratory of Oral Biology Medicine, Jinan, Shandong 250012, P.R. China.
authors.forename: Zhenkun
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Stomatological College of Shandong University, Jinan, Shandong 250012, P.R. China; Shandong Province Key Laboratory of Oral Biology Medicine, Jinan, Shandong 250012, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
keywords: alkaline phosphatase; collagen type I; differentiation; nano tantalum implants; osteoblast; osteocalcin; proliferation