title: Evaluation of the diuretic activity of the aqueous and 80% methanol extracts of the root of Euclea divinorum Hiern (Ebenaceae) in Sprague Dawley rats.
abstract: BACKGROUND
  Diuretics are drugs that increase the formation of urine and are important for the treatment of various diseases including hypertension and edema. The root decoction of Euclea divinorum has been used as a diuretic agent in the traditional medicine. Therefore, this study was aimed to evaluate the diuretic activity of the crude extracts of the roots of Euclea divinorum in Sprague Dawley rats.
  
  
  METHODS
  The aqueous extract (AE) and 80% methanol extract (80ME) of the plant were prepared using decoction and maceration, respectively. Vehicle (distilled water, 10ml/kg), standard drug (hydrochlorothiazide, 10mg/kg) and three doses (100mg/kg, 200mg/kg and 400mg/kg) of the AE and 80ME were given to male rats by oral gavage. Parameters like urine volume (for 5h), electrolyte concentration and pH were measured (at 5thh) and analyzed. Data were analyzed using one way analysis of variance (ANOVA) followed by Tukey post hoc test. Linear regression was also applied to show the dose dependency nature of the diuretic effect.
  
  
  RESULTS
  The result indicated that the 80ME of the plant significantly (p<0.05) produced diuresis at 200mg/kg and 400mg/kg. Furthermore, the AE produced significant diuresis (p<0.05) at all doses. With regard to the electrolyte excretion, the AE produced significant natriuresis and kaliuresis at all tested doses (p<0.001), while the 80ME showed significant natriuresis and kaliuresis at 200mg/kg (p<0.01) and 400mg/kg (p<0.001). Preliminary phytochemical screening revealed the presence of secondary metabolites, including saponins, flavonoids, glycosides, steroids, tannins and terpinoids in both extracts. These constituents might be responsible for the diuretic activity of Euclea divinorum. Both extracts were also found to be safe at 2000mg/kg on the acute toxicity study.
  
  
  CONCLUSIONS
  This finding provides a scientific support for the acclaimed traditional use of the roots of Euclea divinorum as a diuretic agent.
authors.affiliation: Department of Pharmacology, College of Medicine and Health Sciences, Dilla University, Ethiopia.
authors.forename: Beharu
authors.initials: B
authors.lastname: Woldemedhin
authors.affiliation: Department of Pharmacology and Clinical Pharmacy, School of Pharmacy, College of Health Sciences, Addis Ababa University, Ethiopia.
authors.forename: Teshome
authors.initials: T
authors.lastname: Nedi
authors.affiliation: Department of Pharmacology and Clinical Pharmacy, School of Pharmacy, College of Health Sciences, Addis Ababa University, Ethiopia.
authors.forename: Workineh
authors.initials: W
authors.lastname: Shibeshi
authors.affiliation: Department of Pharmacology, School of Pharmacy, College of Health and Medical Sciences, Haramaya University, Ethiopia. Electronic address: mekonnensisay27@yahoo.com.
authors.forename: Mekonnen
authors.initials: M
authors.lastname: Sisay
mesh_terms: D000818:Animals; D004232:Diuretics / Q000494:pharmacology* / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D031262:Ebenaceae / Q000737:chemistry* / Q000633:toxicity; D004573:Electrolytes / Q000032:analysis / Q000652:urine; D005260:Female; D008297:Male; D000432:Methanol; D009318:Natriuresis / Q000187:drug effects; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology* / Q000633:toxicity; D018517:Plant Roots / Q000737:chemistry; D011188:Potassium / Q000652:urine; D051381:Rats; D017207:Rats, Sprague-Dawley; D012997:Solvents; D014563:Urodynamics / Q000187:drug effects; D014867:Water
chemical_list: D004232:Diuretics; D004573:Electrolytes; D010936:Plant Extracts; D012997:Solvents; D014867:Water; D011188:Potassium; D000432:Methanol
keywords: 80ME; Aqueous; Diuresis; Euclea divinorum; HCT; Kaluresis; Natriuresis