title: Effect of lipid-bound apolipoprotein A-I cysteine mutant on ATF3 in RAW264.7 cells.
abstract: Activating transcription factor 3 (ATF3) is a TLR-induced repressor that plays an important role in the inhibition of specific inflammatory signals. We previously constructed recombinant high density lipoproteins (rHDL) (including rHDLWT, rHDLM, rHDL228and rHDL74) and found that rHDL74had a strong anti-inflammatory ability. In the present study, we investigate the roles of recombinant apolipoprotein A-I (ApoA-I) (rHDLWT) and its cysteine mutant HDLs (rHDLM, rHDL228and rHDL74) on ATF3 function in RAW264.7 cells stimulated by lipopolysaccharide. Our results showed that compared with the LPS group, rHDL74can decrease the level of TNF-α and IL-6, whereas rHDL228increases their expression levels. RT-PCR and Western blotting results showed that compared with the LPS group, rHDL74, rHDLWTand rHDLMcan markedly increase the expression level of ATF3, whereas the level of ATF3 decreases in the rHDL228group. In summary, the different anti-inflammatory mechanisms of the ApoA-I cysteine mutants might be associated with the regulation of ATF3 level.
authors.affiliation: Biological Specimen Bank, The Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Biochemistry, Medical College, Qingdao University, Qingdao, Shandong, China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Opthalmology, The Affiliated Hospital of Medical College Qingdao University, 16 Jiangsu Road, Qingdao, China.
authors.forename: Shaoyou
authors.initials: S
authors.lastname: Jia
authors.affiliation: Biological Specimen Bank, The Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Qingzhe
authors.initials: Q
authors.lastname: Dong
authors.affiliation: Biological Specimen Bank, The Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Yuanbin
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Stomatology, Qingdao Municipal Hospital, Qingdao, China wylapollo@163.com lshl97@163.com qingyi001@126.com.
authors.forename: Shulai
authors.initials: S
authors.lastname: Lu
authors.affiliation: Department of Biochemistry, Medical College, Qingdao University, Qingdao, Shandong, China wylapollo@163.com lshl97@163.com qingyi001@126.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Hou
mesh_terms: D051700:Activating Transcription Factor 3 / Q000276:immunology*; D000818:Animals; D016632:Apolipoprotein A-I / Q000235:genetics / Q000276:immunology*; D003545:Cysteine / Q000235:genetics / Q000276:immunology; D006801:Humans; D015850:Interleukin-6 / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D017354:Point Mutation; D000067996:RAW 264.7 Cells; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: C505693:APOA1 protein, human; D051700:Activating Transcription Factor 3; D016632:Apolipoprotein A-I; C103002:Atf3 protein, mouse; D015850:Interleukin-6; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D003545:Cysteine
keywords: ATF3; inflammatory; lipopolysaccharide; rHDL