title: Involvement of water channel Aquaporin 5 in H2S-induced pulmonary edema.
abstract: Acute exposure to hydrogen sulfide (H2S) poses a significant threat to life, and the lung is one of the primary target organs of H2S. However, the mechanisms involved in H2S-induced acute pulmonary edema are poorly understood. This study aims to investigate the effects of H2S on the expression of water channel aquaporin 5 (AQP5) and to elucidate the signaling pathways involved in AQP5 regulation. In an in vivo study, C57BL6 mice were exposed to sub-lethal concentrations of inhaled H2S, and histological injury of the lungs and ultrastructure injury of the epithelial cells were evaluated. With real-time PCR and western blot assays, we found that H2S exposure contributed to a significant decrease in AQP5 expression both in murine lung tissue and the A549 cell line, and the ERK1/2 and p38 MAPK signaling pathways were demonstrated to be implicated in AQP5 regulation. Therefore, adjusting AQP5 protein levels could be considered a therapeutic strategy for the treatment of APE induced by H2S and other hazardous gases.
authors.affiliation: Department of Emergency Medicine, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu, 210029, China.
authors.forename: Chunyang
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Emergency Medicine, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu, 210029, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Key Lab of Modern Toxicology (NJMU), Ministry of Education. Department of Toxicology, School of Public Health, Nanjing Medical University, 818 Tianyuan East Road, Nanjing, Jiangsu, 211166, China.
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Department of Emergency Medicine, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu, 210029, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Xing
authors.affiliation: Department of Emergency Medicine, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu, 210029, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Occupational Disease Prophylactic-Therapeutic Institution, Jiangsu Provincial Center for Disease Prevention and Control, 122 Heban Cun, Nanjing, Jiangsu, 210028, China.
authors.forename: Baoli
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Department of Occupational Disease Prophylactic-Therapeutic Institution, Jiangsu Provincial Center for Disease Prevention and Control, 122 Heban Cun, Nanjing, Jiangsu, 210028, China.
authors.forename: Hengdong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Lab of Modern Toxicology (NJMU), Ministry of Education. Department of Toxicology, School of Public Health, Nanjing Medical University, 818 Tianyuan East Road, Nanjing, Jiangsu, 211166, China. Electronic address: wangjun@njmu.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Emergency Medicine, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu, 210029, China. Electronic address: zhangjsojz@163.com.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000072283:A549 Cells; D000818:Animals; D051402:Aquaporin 5 / Q000235:genetics / Q000378:metabolism*; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D006801:Humans; D006862:Hydrogen Sulfide / Q000633:toxicity*; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D011654:Pulmonary Edema / Q000139:chemically induced* / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D051402:Aquaporin 5; D012333:RNA, Messenger; D020928:Mitogen-Activated Protein Kinases; D006862:Hydrogen Sulfide
keywords: Aquaporin 5; ERK1/2; Hydrogen sulfide; JNK; Pulmonary edema; p38 MAPK