title: Multimodality MRI assessment of grey and white matter injury and blood-brain barrier disruption after intracerebral haemorrhage in mice.
abstract: In this study, we examined injury progression after intracerebral haemorrhage (ICH) induced by collagenase in mice using a preclinical 11.7 Tesla MRI system. On T2-weighted MRI, lesion and striatal volumes were increased on day 3 and then decreased from days 7 to 28. On day 3, with an increase in striatal water content, vasogenic oedema in the perihaematomal region presented as increased T2 and increased apparent diffusion coefficient (ADC) signal. With a synchronous change in T2 and ADC signals, microglial activation peaked on day 3 in the same region and decreased over time. Iron deposition appeared on day 3 around the haematoma border but did not change synchronously with ADC signals. Vascular permeability measured by Evans blue extravasation on days 1, 3, and 7 correlated with the T1-gadolinium results, both of which peaked on day 3. On diffusion tensor imaging, white matter injury was prominent in the corpus callosum and internal capsule on day 3 and then partially recovered over time. Our results indicate that the evolution of grey/white matter injury and blood-brain barrier disruption after ICH can be assessed with multimodal MRI, and that perihaematomal vasogenic oedema might be attributable to microglial activation, iron deposition, and blood-brain barrier breakdown.
authors.affiliation: Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Institute of Neuroscience, Department of Neurology, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.|Department of Anesthesiology/Critical Care Medicine, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Anesthesiology/Critical Care Medicine, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Anesthesiology/Critical Care Medicine, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
authors.forename: Zhongyu
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology/Critical Care Medicine, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
authors.forename: Cunfang
authors.initials: C
authors.lastname: Qi
authors.affiliation: Department of Anesthesiology/Critical Care Medicine, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Han
authors.affiliation: Department of Anesthesiology/Critical Care Medicine, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
authors.forename: Xi
authors.initials: X
authors.lastname: Lan
authors.affiliation: Department of Anesthesiology/Critical Care Medicine, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
authors.forename: Jieru
authors.initials: J
authors.lastname: Wan
authors.affiliation: Department of Anesthesiology/Critical Care Medicine, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
authors.forename: Wenzhu
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology/Critical Care Medicine, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Radiology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Hou
authors.affiliation: Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Institute of Neuroscience, Department of Neurology, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Gao
authors.affiliation: Department of Anesthesiology/Critical Care Medicine, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.|Department of Neurology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors.forename: J Ricardo
authors.initials: JR
authors.lastname: Carhuapoma
authors.affiliation: Department of Radiology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors.forename: Susumu
authors.initials: S
authors.lastname: Mori
authors.affiliation: Bernard and Irene Schwartz Center for Biomedical Imaging, Department of Radiology, New York University School of Medicine, New York, NY 10016, USA.
authors.forename: Jiangyang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology/Critical Care Medicine, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000000981:diagnostic imaging* / Q000503:physiopathology; D002543:Cerebral Hemorrhage / Q000000981:diagnostic imaging* / Q000503:physiopathology; D003337:Corpus Callosum / Q000000981:diagnostic imaging / Q000503:physiopathology; D056324:Diffusion Tensor Imaging; D004195:Disease Models, Animal; D066128:Gray Matter / Q000000981:diagnostic imaging* / Q000293:injuries / Q000503:physiopathology; D006801:Humans; D008279:Magnetic Resonance Imaging; D051379:Mice; D064847:Multimodal Imaging; D066127:White Matter / Q000000981:diagnostic imaging* / Q000293:injuries / Q000503:physiopathology