title: Quantitative Analysis in Combination with Fingerprint Technology and Chemometric Analysis Applied for Evaluating Six Species of WildParisUsing UHPLC-UV-MS.
abstract: A fast method was developed by ultra high performance liquid chromatography (UHPLC) for simultaneous determination of polyphyllin I and polyphyllin II. Chemometric analyses including principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) based on UHPLC chromatography were used to evaluate 38 batches from six species ofParis. Variable importance of projection was applied to select important peaks. Meanwhile, similarity analysis of UHPLC fingerprint was used to evaluate the sample ofParis polyphylla yunnanensis(PPY) andP. axialis(PA). The results indicated that the total content of saponins in PPY and PA collected from Baoshan City of Yunnan Province above 8.07 mg/g was stronger than that from other areas of the rest of species. PLS-DA showed better performance than PCA with regard to classifying the samples. Retention time during 20-27 minutes of UHPLC was screened as significant peak for distinguishingParisof different species and original geography. All of PPY and PA with similarity value were more than 0.80. It indicated that quantitative analysis combined with chemometric and similarity analyses could evaluate the different species ofPariseffectively and comprehensively.
authors.affiliation: College of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China; Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China; Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming 650200, China.
authors.forename: Yuangui
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China; Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming 650200, China.
authors.forename: Ji
authors.identifier: 0000-0002-7737-0785
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China; Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming 650200, China.
authors.forename: Hang
authors.identifier: 0000-0003-1735-7623
authors.initials: H
authors.lastname: Jin
authors.affiliation: College of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China; Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China; Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming 650200, China.
authors.forename: Jinyu
authors.identifier: 0000-0002-1092-0623
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China; Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China; Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming 650200, China.
authors.forename: Yuanzhong
authors.identifier: 0000-0001-5376-757X
authors.initials: Y
authors.lastname: Wang