title: The Polyherbal Wattana Formula Displays Anti-Amyloidogenic Properties by Increasing α-Secretase Activities.
abstract: Alzheimer's disease is characterized by the deposition of insoluble amyloid-β peptides produced from the β-amyloid precursor protein (βAPP). Because α-secretase cleavage by ADAM10 and ADAM17 takes place in the middle of Aβ, its activation is considered as a promising anti-AD therapeutic track. Here we establish that the polyherbal Wattana formula (WNF) stimulates sAPPα production in cells of neuronal and non-neuronal origins through an increase of both ADAM10 and ADAM17 catalytic activities with no modification of BACE1 activity and expression. This effect is blocked by specific inhibition or genetic depletion of these disintegrins and we show that WNF up-regulates ADAM10 transcription and ADAM17 maturation. In addition, WNF reduces Aβ40 and Aβ42 generation in human cell lines. Altogether, WNF presents all the characteristics of a potent preventive anti-Alzheimer formula. Importantly, this natural recipe, currently prescribed to patients for the treatment of other symptoms without any secondary effect, can be tested immediately for further clinical studies.
authors.affiliation: Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.
authors.forename: Htut Htut
authors.initials: HH
authors.lastname: Htoo
authors.affiliation: Center of Applied Thai Traditional Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Suveerawan
authors.initials: S
authors.lastname: Limsuvan
authors.affiliation: Center of Applied Thai Traditional Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Onusa
authors.initials: O
authors.lastname: Thamsermsang
authors.affiliation: Institut des Biomolécules Max Mousseron, UMR5247 CNRS/Université de Montpellier/ENSCM, Faculté de Pharmacie, Montpellier, France.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Hernandez
authors.affiliation: Université Côte d'Azur, INSERM, CNRS, IPMC, Laboratory of excellence DistALZ, Sophia-Antipolis, Valbonne, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Checler
authors.affiliation: Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.|Center for Neuroscience and Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok, Thailand.|Research Center for Neuroscience, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.
authors.forename: Piyarat
authors.initials: P
authors.lastname: Govitrapong
authors.affiliation: Department of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Narawut
authors.initials: N
authors.lastname: Pakaprot
authors.affiliation: Center of Applied Thai Traditional Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.|Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Pravit
authors.initials: P
authors.lastname: Akarasereenont
authors.affiliation: Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.|Research Center for Neuroscience, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.|Centre National de la Recherche Scientifique, Paris, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Vincent
mesh_terms: D053829:Amyloid Precursor Protein Secretases / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D004789:Enzyme Activation; D029001:Herbal Medicine; D006801:Humans
chemical_list: D053829:Amyloid Precursor Protein Secretases