title: Efficacy of silver/hydrophilic poly(p-xylylene) on preventing bacterial growth and biofilm formation in urinary catheters.
abstract: Catheter associated urinary tract infections (CAUTI), caused by several strains of bacteria, are a common complication for catheterized patients. This may eventually lead to a blockage of the catheter due to the formation of a crystalline or amorphous biofilm. Inhibiting bacteria should result in a longer application time free of complaints. This issue has been investigated using an innovative type of silver-coated catheter with a semipermeable cap layer to prevent CAUTI. In this work, two different types of silver catheters were investigated, both of which were capped with poly(p-xylylene) (PPX-N) and exhibited different surface properties that completely changed their wetting conduct with water. The contact angle of conventionally deposited PPX-N is approximately 80°. After O2plasma treatment, the contact angle drops to approximately 30°. These two systems, Ag/PPX-N and Ag/PPX-N-O2, were tested in synthetic urine at a body temperature of 37 °C. First, the optical density and the inhibition zones of both bacteria strains (Escherichia coli and Staphylococcus cohnii) were examined to confirm the antibacterial effect of these silver-coated catheters. Afterward, the efficacy of silver catheters with different treatments of biofilm formed by E. coli and S. cohnii were tested with crystal violet staining assays. To estimate the life cycles of silver/PPX-catheters, the eluted amount of silver was assessed at several time intervals by anodic stripping voltammetry. The silver catheter with hydrophilic PPX-N coating limited bacterial growth in synthetic urine and prevented biofilm formation. The authors attribute the enhanced bacteriostatic effect to increased silver ion release detected under these conditions. With this extensive preparatory analytic work, the authors studied the ability of the two different cap layers (without silver), PPX-N and oxygen plasma treated PPX-N, to control the growth of a crystalline biofilm by measuring the concentrations of the Ca2+and Mg2+ions after exposure of the catheters to saturated urine for 24 h. The higher concentrations of Ca2+and Mg2+in the precipitates on the PPX-N catheters indicates that the hydrophilic PPX-N coating is superior to the simple PPX-N coating, with regard to the formation of a crystalline biofilm. Moreover, hydrophilic PPX-N as a cap layer may promote wettability and increase silver ion release rate and thus reduce the adhesion of suspended crystals to the catheter. Reduced bacterial growth and reduced adhesion may help to prevent CAUTI.
authors.affiliation: Laboratory for Surface Refinement and Thin Film Technology, Munich University of Applied Sciences, Munich D-80335, Bavaria, Germany.
authors.forename: Hamideh
authors.initials: H
authors.lastname: Heidari Zare
authors.affiliation: Laboratory for Surface Refinement and Thin Film Technology, Munich University of Applied Sciences, Munich D-80335, Bavaria, Germany.
authors.forename: Viktorija
authors.initials: V
authors.lastname: Juhart
authors.affiliation: Laboratory for Surface Refinement and Thin Film Technology, Munich University of Applied Sciences, Munich D-80335, Bavaria, Germany.
authors.forename: Attila
authors.initials: A
authors.lastname: Vass
authors.affiliation: Laboratory for Surface Refinement and Thin Film Technology, Munich University of Applied Sciences, Munich D-80335, Bavaria, Germany.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Franz
authors.affiliation: University Hospital of the State of Schleswig-Holstein at Lübeck, Lübeck D-23538, Schleswig-Holstein, Germany.
authors.forename: Dieter
authors.initials: D
authors.lastname: Jocham
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D011108:Polymers / Q000494:pharmacology*; D012834:Silver / Q000494:pharmacology*; D013210:Staphylococcus / Q000187:drug effects* / Q000502:physiology; D013499:Surface Properties; D062885:Urinary Catheters / Q000382:microbiology*; D014992:Xylenes / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D011108:Polymers; D014992:Xylenes; C513670:poly(p-xylylene); D012834:Silver