title: miR-486-3p, miR-139-5p, and miR-21 as Biomarkers for the Detection of Oral Tongue Squamous Cell Carcinoma.
abstract: Oral tongue squamous cell carcinoma (TSCC) is a complex disease with extensive genetic and epigenetic defects, including microRNA deregulation. The aims of the present study were to test the feasibility of performing the microRNA profiling analysis on archived TSCC specimens and to assess the potential diagnostic utility of the identified microRNA biomarkers for the detection of TSCC. TaqMan array-based microRNA profiling analysis was performed on 10 archived TSCC samples and their matching normal tissues. A panel of 12 differentially expressed microRNAs was identified. Eight of these differentially expressed microRNAs were validated in an independent sample set. A random forest (RF) classification model was built with miR-486-3p, miR-139-5p, and miR-21, and it was able to detect TSCC with a sensitivity of 100% and a specificity of 86.7% (overall error rate = 6.7%). As such, this study demonstrated the utility of the archived clinical specimens for microRNA biomarker discovery. The feasibility of using microRNA biomarkers (miR-486-3p, miR-139-5p, and miR-21) for the detection of TSCC was confirmed.
authors.affiliation: Center for Molecular Biology of Oral Diseases, Department of Periodontics, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA.
authors.forename: Zujian
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Biostatistics and Bioinformatics, Rollins School of Public Health, Emory University, Atlanta, GA, USA.
authors.forename: Tianwei
authors.initials: T
authors.lastname: Yu
authors.affiliation: Department of Pathology, College of Medicine, University of Illinois at Chicago, Chicago, IL, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Cabay
authors.affiliation: Center for Molecular Biology of Oral Diseases, Department of Periodontics, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Center for Molecular Biology of Oral Diseases, Department of Periodontics, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA.; Department of Biosciences, COMSATS Institute of Information and Technology, Islamabad, Pakistan.
authors.forename: Ishrat
authors.initials: I
authors.lastname: Mahjabeen
authors.affiliation: Department of Oral Biology, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA.
authors.forename: Xianghong
authors.initials: X
authors.lastname: Luan
authors.affiliation: Department of Bioengineering, College of Engineering, University of Illinois at Chicago, Chicago, IL, USA.
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Bioengineering, College of Engineering, University of Illinois at Chicago, Chicago, IL, USA.; UIC Cancer Center, Graduate College, University of Illinois at Chicago, Chicago, IL, USA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Center for Molecular Biology of Oral Diseases, Department of Periodontics, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA.; UIC Cancer Center, Graduate College, University of Illinois at Chicago, Chicago, IL, USA.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Zhou
keywords: biomarker; formalin-fixed paraffin-embedded; miR-139-5p; miR-21; miR-486-3p; microRNA; tongue squamous cell carcinoma