title: Interaction of octahedral ruthenium(II) polypyridyl complex [Ru(bpy)2(PIP)]2+with poly(U)·poly(A)*poly(U) triplex: Increasing third-strand stabilization of the triplex without affecting the stability of the duplex.
abstract: Triple-helical RNA are of interest because of possible biological roles as well as the potential therapeutic uses of these structures, while the stability of triplexes is usually weaker than that of the Watson-Crick base pairing duplex strand due to the electrostatic repulsion between three polyanionic strands. Therefore, how to increase the stability of the specific sequences of triplexes are of importance. In this paper the binding of a Ru(II) complex, [Ru(bpy)2(PIP)]2+(bpy=2.2'-bipyridine, PIP=2-phenyl-1H-imidazo[4,5-f]- [1,10]-phenanthroline), with poly(U)·poly(A)*poly(U) triplex has been investigated by spectrophotometry, spectrofluorometry, viscosimetry and circular dichroism. The results suggest that [Ru(bpy)2(PIP)]2+as a metallointercalator can stabilize poly(U)·poly(A)*poly(U) triplex (where · denotes the Watson-Crick base pairing and * denotes the Hoogsteen base pairing),while it stabilizes third-strand with no obvious effect on the duplex of poly(U)·poly(A), reflecting the binding of this complex with the triplex is favored by the Hoogsteen paired poly(U) third strand to a great extent.
authors.affiliation: College of Chemistry, Xiangtan University, Xiangtan 411105, PR China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: College of Chemistry, Xiangtan University, Xiangtan 411105, PR China.
authors.forename: Mengna
authors.initials: M
authors.lastname: Peng
authors.affiliation: College of Chemistry, Xiangtan University, Xiangtan 411105, PR China.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Lab of Environment-friendly Chemistry and Application in Ministry of Education, Xiangtan University, Xiangtan 411105, PR China. Electronic address: lfwyxh@yeah.net.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Tan
mesh_terms: D015082:2,2'-Dipyridyl / Q000737:chemistry*; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009942:Organometallic Compounds / Q000737:chemistry*; D011072:Poly U / Q000737:chemistry*; D011485:Protein Binding; D012313:RNA / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*
chemical_list: D009942:Organometallic Compounds; D011072:Poly U; D015082:2,2'-Dipyridyl; D012313:RNA; D012428:Ruthenium
keywords: RNA triplex; Ruthenium(II) polypyridyl complex; Stabilization