title: Protein Nanoparticle Formation Using a Circularly Permuted α-Helix-Rich Trimeric Protein.
abstract: We here report the production of highly spherical protein nanoparticles based on the domain-swapping oligomerization of a circularly permuted trimeric protein, major histocompatibility complex (MHC) class II associated chaperonin. The size distribution of the nanoparticles can be adjusted to between 40 and 100 nm in diameter, and thus, these particles are suitable as drug carriers following purification under basic conditions. Our approach involves no harsh treatments and could provide an alternative approach for protein nanoparticle formation.
authors.affiliation: Department of Bioscience and Informatics, Faculty of Science and Technology, Keio University , 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan.
authors.forename: Norifumi
authors.identifier: 0000-0002-0981-9499
authors.initials: N
authors.lastname: Kawakami
authors.affiliation: Department of Bioscience and Informatics, Faculty of Science and Technology, Keio University , 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kondo
authors.affiliation: Department of Bioscience and Informatics, Faculty of Science and Technology, Keio University , 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Muramatsu
authors.affiliation: Department of Bioscience and Informatics, Faculty of Science and Technology, Keio University , 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Miyamoto
mesh_terms: D000949:Histocompatibility Antigens Class II / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D000072756:Protein Conformation, alpha-Helical; D055503:Protein Multimerization
chemical_list: D000949:Histocompatibility Antigens Class II