title: ChMob2 binds to ChCbk1 and promotes virulence and conidiation of the fungal pathogen Colletotrichum higginsianum.
abstract: BACKGROUND
  Mob family proteins are conserved between animals, plants and fungi and are essential for the activation of NDR kinases that control crucial cellular processes like cytokinesis, proliferation and morphology.
  
  
  RESULTS
  We identified a hypomorphic allele of ChMOB2 in a random insertional mutant (vir-88) of the hemibiotrophic ascomycete fungus Colletotrichum higginsianum. The mutant is impaired in conidiation, host penetration and virulence on Arabidopsis thaliana. ChMob2 binds to and co-localizes with the NDR/LATS kinase homolog ChCbk1. Mutants in the two potential ChCbk1 downstream targets ChSSD1 and ChACE2 show defects in pathogenicity. The genome of C. higginsianum encodes two more Mob proteins. While we could not detect any effect on pathogenicity in ΔChmob3 mutants, ChMob1 is involved in conidiation, septae formation and virulence.
  
  
  CONCLUSIONS
  This study shows that ChMob2 binds to the conserved NDR/LATS Kinase ChCbk1 and plays an important role in pathogenicity of Colletotrichum higginsianum on Arabidopsis thaliana.
authors.affiliation: Department of Biology, Division of Biochemistry, Friedrich-Alexander University Erlangen-Nuremberg, Staudtstrasse 5, 91058, Erlangen, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Schmidpeter
authors.affiliation: Department of Biology, Division of Biochemistry, Friedrich-Alexander University Erlangen-Nuremberg, Staudtstrasse 5, 91058, Erlangen, Germany.
authors.forename: Marlis
authors.initials: M
authors.lastname: Dahl
authors.affiliation: Department of Biology, Division of Biochemistry, Friedrich-Alexander University Erlangen-Nuremberg, Staudtstrasse 5, 91058, Erlangen, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Hofmann
authors.affiliation: Department of Biology, Division of Biochemistry, Friedrich-Alexander University Erlangen-Nuremberg, Staudtstrasse 5, 91058, Erlangen, Germany. christian.koch@fau.de.
authors.forename: Christian
authors.identifier: http://orcid.org/0000-0001-8407-4952
authors.initials: C
authors.lastname: Koch
mesh_terms: D017360:Arabidopsis / Q000382:microbiology; D020231:Colletotrichum / Q000235:genetics* / Q000472:pathogenicity; D004269:DNA, Bacterial; D005656:Fungal Proteins / Q000235:genetics* / Q000502:physiology*; D005800:Genes, Fungal / Q000235:genetics; D020928:Mitogen-Activated Protein Kinases; D016254:Mutagenesis, Insertional; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000382:microbiology; D014774:Virulence / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D005656:Fungal Proteins; C036483:T-DNA; D020928:Mitogen-Activated Protein Kinases; C409987:cmk1 protein, Colletotrichum lagenarium
keywords: ATMT; Ace2; Colletotrichum higginsianum; Conidiation; Cts1; Mob1; Mob2; Mob3; NDR Kinase Cbk1; Phytopathogenic ascomycete fungus; Ssd1