title: Antioxidant capacity and identification of the constituents of ethyl acetate fraction from Rhus verniciflua Stokes by HPLC-MS.
abstract: Ethyl acetate fraction (EAF) from Rhus verniciflua Stokes is an important source of bioactive compounds. The aim of this study was the tentative identification and quantification of phenolic compounds, comparison of the phenolic structure-antioxidant activity relationships. Twelve compounds of EAF belonging to polyphenol types were detected by high performance liquid chromatography and analysed on line with negative ion electrospray ionisation tandem mass spectrometry, which were ethoxy 3-hydroxy benzoic acid, gallic acid (GA), 3,4-dihydroxy amygdalic acid, gallic acid cetyl ester, protocatechuic acid (PA), fustin, ethyl gallate (EG), garbanzol, fisetin, sulfuretin, butin and 3,7-dihydroxyflavanone-4'-rhamnoside. The antioxidant activity were evaluated based on the different types of radical scavenging capacities, i.e. DPPH·, ABTS·+ and OH. The antioxidant capacity of EAF mainly depended on the GA, EG, PA, fisetin, sulfuretin and butin. The phenolics exhibited a dose-dependent behaviour and high antioxidant ability.
authors.affiliation: a Institute of Chemical Industry of Forest Products , CAF , Nanjing , China.|b Key and Open Laboratory on Forest Chemical Engineering , SFA , Nanjing , China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Chen
authors.affiliation: a Institute of Chemical Industry of Forest Products , CAF , Nanjing , China.|b Key and Open Laboratory on Forest Chemical Engineering , SFA , Nanjing , China.|c Institute of New Technology of Forestry , CAF , Beijing , China.
authors.forename: Chengzhang
authors.initials: C
authors.lastname: Wang
authors.affiliation: a Institute of Chemical Industry of Forest Products , CAF , Nanjing , China.|b Key and Open Laboratory on Forest Chemical Engineering , SFA , Nanjing , China.|c Institute of New Technology of Forestry , CAF , Beijing , China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhou
authors.affiliation: a Institute of Chemical Industry of Forest Products , CAF , Nanjing , China.|b Key and Open Laboratory on Forest Chemical Engineering , SFA , Nanjing , China.
authors.forename: Ran
authors.initials: R
authors.lastname: Tao
authors.affiliation: a Institute of Chemical Industry of Forest Products , CAF , Nanjing , China.|b Key and Open Laboratory on Forest Chemical Engineering , SFA , Nanjing , China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Ye
authors.affiliation: a Institute of Chemical Industry of Forest Products , CAF , Nanjing , China.|b Key and Open Laboratory on Forest Chemical Engineering , SFA , Nanjing , China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Li
mesh_terms: D000085:Acetates; D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology; D001572:Benzofurans / Q000032:analysis; D001578:Benzopyrans; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis; D044948:Flavonols; D016166:Free Radical Scavengers; D010636:Phenols / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry*; D029045:Rhus / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000085:Acetates; D000975:Antioxidants; D001572:Benzofurans; D001578:Benzopyrans; D005419:Flavonoids; D044948:Flavonols; D016166:Free Radical Scavengers; D010636:Phenols; D010936:Plant Extracts; C051437:butin; C522894:fustin; C007650:ethyl acetate; C054989:sulfuretin; C017875:fisetin
keywords: HPLC-MS; Rhus verniciflua Stokes; antioxidant activity; ethyl acetate fraction; phenolic compounds