title: Conjugated polymer with carboxylate groups-Hg2+system as a turn-on fluorescence probe for label-free detection of cysteine-containing compounds.
abstract: In this work, a turn on fluorescent sensor, based on Hg2+coordination conjugated polymer, was developed to detect cysteine-containing compounds. The fluorescence of conjugated polymer (poly(2,5-bis (sodium 4-oxybutyrate) -1,4 - phenylethynylene-alt-1,4-phenyleneethynylene; PPE-OBS) would be quenched by Hg2+because of the coordination-induced aggregation and electron transfers of PPE-OBS toward Hg2+. When there were some cysteine-containing compounds in PPE-OBS-Hg2+system, the fluorescence of PPE-OBS would be recovered. It indicated that the PPE-OBS-Hg2+system could be used to detect cysteine-containing compounds. Under the optimized conditions, the experiment results showed that there were particularly linear range, high sensitivity and selectivity over other amino acids. The limit of detection (LOD) of cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) were 0.725μmolL-1, 0.982μmolL-1and 1.21μmolL-1by using this sensor. In addition, Cys standard recovery in several green tea drink and honey samples was also demonstrated. The recovery of Cys was range from 96.3 to 105.0% and RSD was less than 3.25%. The satisfactory results demonstrated that the proposed method could be as a potential fluorescent method for determining cysteine-containing compounds in real samples.
authors.affiliation: College of Chemistry, Jilin University, Jiefang Road 2519, Changchun 130021, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Mi
authors.affiliation: College of Chemistry, Jilin University, Jiefang Road 2519, Changchun 130021, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Guan
authors.affiliation: College of Chemistry, Jilin University, Jiefang Road 2519, Changchun 130021, China.
authors.forename: Jilin
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Chemistry, Jilin University, Jiefang Road 2519, Changchun 130021, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Shan
authors.affiliation: College of Chemistry, Jilin University, Jiefang Road 2519, Changchun 130021, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fei
authors.affiliation: College of Chemistry, Jilin University, Jiefang Road 2519, Changchun 130021, China.
authors.forename: Yanfu
authors.initials: Y
authors.lastname: Huan
authors.affiliation: College of Chemistry, Jilin University, Jiefang Road 2519, Changchun 130021, China. Electronic address: fenggd@jlu.edu.cn.
authors.forename: Guodong
authors.initials: G
authors.lastname: Feng
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002264:Carboxylic Acids / Q000737:chemistry*; D003545:Cysteine / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008628:Mercury / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling; D013696:Temperature; D013997:Time Factors
chemical_list: D000596:Amino Acids; D002264:Carboxylic Acids; D005456:Fluorescent Dyes; D011108:Polymers; D008628:Mercury; D003545:Cysteine
keywords: Cysteine-containing compounds; Fluorescence sensor; Hg(2+); Water-soluble conjugated polymer