title: The Arabidopsis RING-Type E3 Ligase TEAR1 Controls Leaf Development by Targeting the TIE1 Transcriptional Repressor for Degradation.
abstract: The developmental plasticity of leaf size and shape is important for leaf function and plant survival. However, the mechanisms by which plants form diverse leaves in response to environmental conditions are not well understood. Here, we identified TIE1-ASSOCIATED RING-TYPE E3 LIGASE1 (TEAR1) and found that it regulates leaf development by promoting the degradation of TCP INTERACTOR-CONTAINING EAR MOTIF PROTEIN1 (TIE1), an important repressor of CINCINNATA (CIN)-like TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) transcription factors, which are key for leaf development. TEAR1 contains a typical C3H2C3-type RING domain and has E3 ligase activity. We show that TEAR1 interacts with the TCP repressor TIE1, which is ubiquitinated in vivo and degraded by the 26S proteasome system. We demonstrate that TEAR1 is colocalized with TIE1 in nuclei and negatively regulates TIE1 protein levels. Overexpression ofTEAR1rescued leaf defects caused byTIE1overexpression, whereas disruption of TEAR1 resulted in leaf phenotypes resembling those caused byTIE1overexpression or TCP dysfunction. Deficiency inTEARpartially rescued the leaf defects ofTCP4overexpression line and enhanced the wavy leaf phenotypes ofjaw-5DWe propose that TEAR1 positively regulates CIN-like TCP activity to promote leaf development by mediating the degradation of the TCP repressor TIE1.
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China.
authors.forename: Jinzhe
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China.
authors.forename: Baoye
authors.initials: B
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China.
authors.forename: Rongrong
authors.initials: R
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China.
authors.forename: Jianhui
authors.identifier: 0000-0003-1532-0448
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China.
authors.forename: Mingxin
authors.identifier: 0000-0002-4296-5033
authors.initials: M
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China.
authors.forename: Zhuoyao
authors.identifier: 0000-0003-4201-4649
authors.initials: Z
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China qingenji@pku.edu.cn.|School of Advanced Agricultural Sciences, Peking University, Beijing 100871, People's Republic of China.
authors.forename: Genji
authors.identifier: 0000-0002-6995-5126
authors.initials: G
authors.lastname: Qin
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D018519:Meristem / Q000378:metabolism; D008957:Models, Genetic; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D029681:Arabidopsis Proteins; D012097:Repressor Proteins; C585558:TIE1 protein, Arabidopsis; C000625898:TEAR1 protein, Arabidopsis; D044767:Ubiquitin-Protein Ligases