title: Cotransfer of regulatory T cells improve the therapeutic effectiveness of mesenchymal stem cells in treating a colitis mouse model.
abstract: Inflammatory bowel disease (IBD) is a severe inflammatory condition in the colon. To date, clinical solutions for this disease have been limited. Mesenchymal stem cells (MSCs), multipotential cells with immune regulation and anti-inflammatory functions, have been applied to treatment of IBD. However, the therapeutic effectiveness of MSCs still needs to be improved. Here, we were interested in whether regulatory T cells (Tregs) could enhance the immune regulation function of MSCs in treatment of mouse colitis. We generated a dextran sulfate sodium (DSS)-induced IBD mouse model. Combined cell therapy with both MSCs and Tregs was able to help increase body weight and preserve a better colon morphology compared with single cell therapy with MSCs or Tregs alone. Further studies demonstrated that combined cell therapy could reduce pro-inflammatory cytokines such as TNF-α,IL-10, IFN-γ, IL-17A, IL-1β and at the same time promote CD3+T cells apoptosis. In conclusion, our study indicates that combined cell therapy could prevent the development of colitis in a mouse model, which may lead to a new effective therapeutic approach for treatment of human IBD.
authors.affiliation: Stomatological Hospital of Chongqing Medical University, Chongqing, P. R. China.|Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, P. R. China.|Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, P. R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Stomatological Hospital of Chongqing Medical University, Chongqing, P. R. China.|Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, P. R. China.|Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, P. R. China.
authors.forename: Tianyu
authors.initials: T
authors.lastname: Zhao
authors.affiliation: Stomatological Hospital of Chongqing Medical University, Chongqing, P. R. China.|Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, P. R. China.|Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, P. R. China.
authors.forename: Deqin
authors.initials: D
authors.lastname: Yang
mesh_terms: D000818:Animals; D017209:Apoptosis; D017252:CD3 Complex; D002454:Cell Differentiation; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D002478:Cells, Cultured; D003092:Colitis / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D003106:Colon / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D018836:Inflammation Mediators / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000276:immunology; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes; D050378:T-Lymphocytes, Regulatory; D016896:Treatment Outcome
chemical_list: D017252:CD3 Complex; D016207:Cytokines; D018836:Inflammation Mediators
keywords: colitis; mesenchymal stem cells; regulatory T cells