title: A chemiluminescent aptasensor based on rolling circle amplification and Co2+/N-(aminobutyl)-N-(ethylisoluminol) functional flowerlike gold nanoparticles for Salmonella typhimurium detection.
abstract: A sensitive steady-state chemiluminescent aptasensor based on rolling circle amplification (RCA) was fabricated for the detection of Salmonella typhimurium. The sensor utilized aptamer modified Fe3O4magnetic nanoparticles (MNPs) as capture probes, aptamer as recognition molecules, and Co2+enhanced N‑(aminobutyl)-N-(ethylisoluminol) (ABEI) functional flowerlike gold nanoparticles (AuNFs) and complementary strand (cDNA) complex (Co2+/ABEI-AuNFs-cDNA) as signal probes. And P-Iodophenol (PIP) was also added to form a typical ABEI- AuNFs-PIP-H2O2steady-state CL system. By virtue of Fe3O4MNPs based solid-phase RCA strategy, S. typhimurium can be first captured by the aptamer immobilized on the surface of Fe3O4MNPs then complex with RCA products to form a sandwich complex. Co2+/ABEI-AuNFs-cDNA signal probes were then assembled on the RCA products to produce and enhance CL signals. Under optimal conditions, the logarithmic correlation between the concentration of S. typhimurium and the CL signal was found to be linear within the range of 32cfumL-1to 3.2×106cfumL-1(R2=0.9921). The limits of detection of the developed method were found to be 10cfumL-1for S. typhimurium. The method was also used to detect S. typhimurium in real pork samples. The results were compared with those obtained from the plate-counting methods and showed good consistency. Therefore, this detection aptasnesor can be a good candidate for sensitive and selective detection of S. typhimurium with simple and effective operations.
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Liling
authors.initials: L
authors.lastname: Hao
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Huajie
authors.initials: H
authors.lastname: Gu
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Nuo
authors.initials: N
authors.lastname: Duan
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Shijia
authors.initials: S
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xia
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China. Electronic address: wangzp@jiangnan.edu.cn.
authors.forename: Zhouping
authors.initials: Z
authors.lastname: Wang
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods*; D003035:Cobalt / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D008165:Luminol / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D021141:Nucleic Acid Amplification Techniques; D012486:Salmonella typhimurium / Q000302:isolation & purification*
chemical_list: D052157:Aptamers, Nucleotide; D003035:Cobalt; D008165:Luminol; D006046:Gold
keywords: Chemiluminescent; Co(2+) enhanced N‑(aminobutyl)-N- (ethylisoluminol) functional flowerlike gold nanoparticles; Rolling circle amplification; Salmonella typhimurium