title: Williamsia spongiae sp. nov., an actinomycete isolated from the marine sponge Amphimedon viridis.
abstract: A novel actinobacterium, designated isolate B138T, was isolated from the marine sponge, Amphimedon viridis, which was collected from Praia Guaecá (São Paulo, Brazil), and its taxonomic position was established using data from a polyphasic study. The organism showed a combination of chemotaxonomic and morphological characteristics consistent with its classification in the genus Williamsia and it formed a distinct phyletic line in the Williamsia 16S rRNA gene tree. It was most closely related to Williamsia serinedens DSM 45037T and Williamsia deligens DSM 44902T (99.0 % 16S rRNA gene sequence similarity) and Williamsia maris DSM 44693T (97.5 % 16S rRNA gene sequence similarity), but was distinguished readily from these strains by the low DNA-DNA relatedness values (62.3-64.4 %) and by the discriminatory phenotypic properties. Based on the data obtained, the isolate B138T (=CBMAI 1094T=DSM 46676T) should be classified as the type strain of a novel species of the genus Williamsia, for which the name Williamsia spongiae sp. nov. is proposed.
authors.affiliation: Centro Pluridisciplinar de Pesquisas Químicas Biológicas e Agrícolas, UNICAMP, Av. Alexandre Cazelatto 999, CEP 13148-218, Paulínia, SP, Brazil.|Programa de Pós-Graduação em Genética e Biologia Molecular, UNICAMP, CP 6109, 13083-970, Campinas, SP, Brazil.
authors.forename: Cláudia Beatriz
authors.initials: CB
authors.lastname: Afonso de Menezes
authors.affiliation: Centro Pluridisciplinar de Pesquisas Químicas Biológicas e Agrícolas, UNICAMP, Av. Alexandre Cazelatto 999, CEP 13148-218, Paulínia, SP, Brazil.
authors.forename: Rafael Sanches
authors.initials: RS
authors.lastname: Afonso
authors.affiliation: Embrapa Meio Ambiente, Rodovia SP 340 Km 127.5, CP 69, CEP 13820-000, Jaguariúna, SP, Brazil.
authors.forename: Wallace Rafael de
authors.initials: WR
authors.lastname: Souza
authors.affiliation: Embrapa Meio Ambiente, Rodovia SP 340 Km 127.5, CP 69, CEP 13820-000, Jaguariúna, SP, Brazil.
authors.forename: Márcia
authors.initials: M
authors.lastname: Parma
authors.affiliation: Embrapa Meio Ambiente, Rodovia SP 340 Km 127.5, CP 69, CEP 13820-000, Jaguariúna, SP, Brazil.
authors.forename: Itamar Soares de
authors.initials: IS
authors.lastname: Melo
authors.affiliation: Embrapa Meio Ambiente, Rodovia SP 340 Km 127.5, CP 69, CEP 13820-000, Jaguariúna, SP, Brazil.|Agrivalle - Biotecnologia Agrícola, Rua Moisés Lopes Silva 50, CEP 37550-000, Pouso Alegre, MG, Brazil.
authors.forename: Tiago Domingues
authors.initials: TD
authors.lastname: Zucchi
authors.affiliation: Programa de Pós-Graduação em Genética e Biologia Molecular, UNICAMP, CP 6109, 13083-970, Campinas, SP, Brazil.|Centro Pluridisciplinar de Pesquisas Químicas Biológicas e Agrícolas, UNICAMP, Av. Alexandre Cazelatto 999, CEP 13148-218, Paulínia, SP, Brazil.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Fantinatti-Garboggini
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001938:Brazil; D004269:DNA, Bacterial / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011161:Porifera / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S