title: HnRNP-L mediates bladder cancer progression by inhibiting apoptotic signaling and enhancing MAPK signaling pathways.
abstract: Heterogeneous nuclear ribonucleoprotein L (hnRNP-L) is a promoter of various kinds of cancers, but its actions in bladder cancer (BC) are unclear. In this study, we investigated the function and the underlying mechanism of hnRNP-L in bladder carcinogenesis. Our results demonstrated that enhanced hnRNP-L expression in BC tissues was associated with poor overall survival of BC patients. Depletion of hnRNP-L significantly suppressed cell proliferation in vitro and inhibited xenograft tumor growth in vivo. Furthermore, downregulation of hnRNP-L resulted in G1-phase cell cycle arrest and enhanced apoptosis accompanied by inhibition of EMT and cell migration. All these cellular changes were reversed by ectopic expression of hnRNP-L. Deletion of hnRNP-L resulted in decreased expression of Bcl-2, enhanced expression of caspases-3, -6 and -9 and inhibition of the MAPK signaling pathway. These findings demonstrate that hnRNP-L contributes to poor prognosis and tumor progression of BC by inhibiting the intrinsic apoptotic signaling and enhancing MAPK signaling pathways.
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P. R. China.
authors.forename: Daojun
authors.initials: D
authors.lastname: Lv
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P. R. China.
authors.forename: Huayan
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Urology, Affiliated Weihai Second Municipal Hospital of Qingdao University, Weihai 264200, P. R. China.
authors.forename: Rongwei
authors.initials: R
authors.lastname: Xing
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P. R. China.
authors.forename: Fangpeng
authors.initials: F
authors.lastname: Shu
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P. R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Lei
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P. R. China.
authors.forename: Chengyong
authors.initials: C
authors.lastname: Lei
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P. R. China.
authors.forename: Xumin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P. R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wan
authors.affiliation: Department of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen, Guangdong Province, 518036, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P. R. China.
authors.forename: Liren
authors.initials: L
authors.lastname: Zhong
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P. R. China.|Department of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen, Guangdong Province, 518036, China.
authors.forename: Xiangming
authors.initials: X
authors.lastname: Mao
authors.affiliation: Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P. R. China.
authors.forename: Yaguang
authors.initials: Y
authors.lastname: Zou
mesh_terms: D017209:Apoptosis / Q000502:physiology; D059447:Cell Cycle Checkpoints; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D018450:Disease Progression; D005260:Female; D034664:Heterogeneous-Nuclear Ribonucleoprotein L / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D020935:MAP Kinase Signaling System; D008297:Male; D008875:Middle Aged; D015398:Signal Transduction; D015854:Up-Regulation; D001749:Urinary Bladder Neoplasms / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D034664:Heterogeneous-Nuclear Ribonucleoprotein L
keywords: MAPK; bladder cancer; epithelial-mesenchymal transition; heterogeneous nuclear ribonucleoprotein L; intrinsic apoptosis