title: Involvement of cancer-derived IgG in the proliferation, migration and invasion of bladder cancer cells.
abstract: It is widely accepted that immunoglobulin (Ig), the classical immune molecule, is extensively expressed in many cell types other than B-cells (non-B-IgG), including some malignant cells. The expression of Ig in malignant cells has been associated with a poor prognosis. In the present study, immunohistochemical analysis detected strong positive staining of IgG in three bladder cancer cell lines, the cancer cells in 77 bladder cancer patient samples and the cells in 3 cystitis glandularis tissue samples, while negative staining was observed in 4 specimens of normal transitional epithelial tissues. Importantly, functional transcripts of IgG with unique VHDJHrearrangement patterns were also found in bladder cancer cells. The knockdown of IgG in bladder cancer cell lines using small interfering RNA significantly inhibited the proliferation, migration and invasion of the cells. Notably, high IgG expression, as determined by immunostaining, was significantly correlated with a high histological grade and recurrence. The results of the present study suggested that IgG expression is involved in the malignant biological behavior and poor prognosis of bladder cancer. Therefore, IgG may serve as a novel target for bladder cancer therapy.
authors.affiliation: Department of Urology, 2nd Clinical Medical College of Peking University, Peking University People's Hospital, Beijing 100044, P.R. China.
authors.forename: Zhengzuo
authors.initials: Z
authors.lastname: Sheng
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University, Beijing 100191, P.R. China; Peking University Center for Human Disease Genomics, Beijing 100191, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Urology, 2nd Clinical Medical College of Peking University, Peking University People's Hospital, Beijing 100044, P.R. China.
authors.forename: Caipeng
authors.initials: C
authors.lastname: Qin
authors.affiliation: Department of Urology, Beijing Jishuitan Hospital, Beijing 100035, P.R. China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Urology, 2nd Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, P.R. China.
authors.forename: Yeqing
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Department of Urology, 2nd Clinical Medical College of Peking University, Peking University People's Hospital, Beijing 100044, P.R. China.
authors.forename: Huaqi
authors.initials: H
authors.lastname: Yin
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University, Beijing 100191, P.R. China; Peking University Center for Human Disease Genomics, Beijing 100191, P.R. China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Qiu
authors.affiliation: Department of Urology, 2nd Clinical Medical College of Peking University, Peking University People's Hospital, Beijing 100044, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
keywords: bladder cancer; cancer progression; immunoglobulin G; prognosis