title: Functional Roles of Pattern Recognition Receptors That Recognize Virus Nucleic Acids in Human Adipose-Derived Mesenchymal Stem Cells.
abstract: Human adipose-derived mesenchymal stem cells (hAD-MSCs) are mesenchymal stem cells with the capability to modulate immune responses. Evidence showing that hAD-MSCs could mediate innate immune responses through pattern recognition receptors (PRRs) is increasing. However, the roles of PRRs in regulating the innate sensing of virus nucleic acids (RNA and DNA) in hAD-MSCs have not yet been investigated. This study focused on the abundant expression of PRRs, including Toll-like receptor 3 (TLR3) and retinoic acid-inducible gene I (RIG-I), which recognize viral RNA, and gamma-interferon inducible protein 16 (IFI16), which recognizes viral DNA in hAD-MSCs. Poly(I:C), a synthetic dsRNA analogy, activated TLR3 and RIG-I and induced the expression of type I interferons (IFN-α/β) and antivirus proteins, including IFN-stimulating gene 15, 2'5'-oligoadenylate synthetase, and Mx GTPase 1 in hAD-MSCs, which were attenuated by the knockdown of each TLR3 or RIG-I. Synthetic herpes simplex viral DNA (HSV60) activated IFI16 and induced the expression of IFN-α/βand antivirus proteins in hAD-MSCs, which were inhibited by the knockdown of IFI16. Both poly(I:C) and HSV60 induced the expression of IFN-α/βthrough the phosphorylation of IFN-regulatory factor 3. All these results indicated that PRRs recognizing virus nucleic acids were expressed and can mediate antivirus responses in hAD-MSCs.
authors.affiliation: School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453003, China; Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang, Henan 453003, China.
authors.forename: Lili
authors.identifier: 0000-0001-5832-1024
authors.initials: L
authors.lastname: Yu
authors.affiliation: School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453003, China; School of Biomedical Engineering, Xinxiang Medical University, Xinxiang, Henan 453003, China.
authors.forename: Yongtao
authors.identifier: 0000-0002-4444-7790
authors.initials: Y
authors.lastname: Xu
authors.affiliation: School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453003, China; Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang, Henan 453003, China.
authors.forename: Fangchao
authors.initials: F
authors.lastname: Wang
authors.affiliation: School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453003, China; Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang, Henan 453003, China.
authors.forename: Can
authors.initials: C
authors.lastname: Yang
authors.affiliation: School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453003, China; Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang, Henan 453003, China.
authors.forename: Guoyan
authors.initials: G
authors.lastname: Liu
authors.affiliation: School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453003, China; Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang, Henan 453003, China.
authors.forename: Xiangfeng
authors.initials: X
authors.lastname: Song
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000071457:DEAD Box Protein 58 / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D006801:Humans; D016898:Interferon-alpha / Q000096:biosynthesis; D016899:Interferon-beta / Q000096:biosynthesis; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*; D011971:Receptors, Immunologic; D051196:Toll-Like Receptor 3 / Q000378:metabolism*
chemical_list: D004279:DNA, Viral; D016898:Interferon-alpha; D009687:Nuclear Proteins; D010750:Phosphoproteins; D012367:RNA, Viral; D011971:Receptors, Immunologic; C495344:TLR3 protein, human; D051196:Toll-Like Receptor 3; C076700:IFI16 protein, human; D016899:Interferon-beta; C452820:RIGI protein, human; D000071457:DEAD Box Protein 58