title: Expression of decorin in intestinal tissues of mice with inflammatory bowel disease and its correlation with autophagy.
abstract: The aim of this study was to investigate the expression of decorin (DCN) in the intestinal tissues of mice with inflammatory bowel disease (IBD) and its correlation with autophagy. The IBD mouse model was created by intrarectal injection of trinitrobenzene sulfonic acid. The pathology of colon tissues of the mice was examined using hematoxylin and eosin staining. Expression of DCN and the proteins associated with autophagy was detected using immunohistochemistry. Normal human colon mucosal epithelial cells (NCM460 cells) were transfected with DCN expression plasmid and the expression of DCN and autophagy-associated proteins was detected by western blot analysis. Cell apoptosis was studied using an Annexin V apoptosis detection assay and intracellular autophagosomes were observed using electron microscopy. The IBD mouse model was successfully established. Thickening, edema and inflammatory cell infiltration of the intestinal wall were observed in the IBD mice. The expression of DCN as well as the autophagy-associated proteins beclin 1 and LC3B, was increased in the intestinal tissues of the IBD mice. Furthermore, in the NCM460 cells transfected with DCN, the expression of beclin 1 and LC3B was upregulated, while p62 expression was downregulated. Intracellular autophagosomes were increased and apoptosis was decreased in the cells with DCN overexpression. Inhibition of autophagy reversed the effects of DCN on apoptosis. Therefore, DCN is able to induce autophagy and protect intestinal cells during the occurrence and development of IBD.
authors.affiliation: Department of General Surgery, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China; Department of General Surgery, 309 Hospital of Chinese People's Liberation Army, Beijing 100091, P.R. China.
authors.forename: Huazhou
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of General Surgery, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China.
authors.forename: Hongqing
authors.initials: H
authors.lastname: Xi
authors.affiliation: Department of General Surgery, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wei
authors.affiliation: Department of General Surgery, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China.
authors.forename: Aizhen
authors.initials: A
authors.lastname: Cai
authors.affiliation: Medical Administration Division of Beijing Chaoyang Hospital, Beijing 100020, P.R. China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of General Surgery, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China; Department of General Surgery, 309 Hospital of Chinese People's Liberation Army, Beijing 100091, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of General Surgery, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of General Surgery, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China.
authors.forename: Yanjing
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of General Surgery, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
keywords: autophagy; decorin; inflammatory bowel disease