title: MIL-101(Cr) as matrix for sensitive detection of quercetin by matrix-assisted laser desorption/ionization mass spectrometry.
abstract: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has been proven as a useful and advanced technique in the identification of polymers and proteins. However, MALDI-TOF-MS still has the unavoidable drawback of self-signal interference with traditional organic matrices, which could suppress and overlap with the analyte signals in the low-mass region. In this work, MIL-101(Cr), a kind of metal-organic frameworks which possess high molecular weight, π-conjugated 3-D structure, coordinately unsaturated chromium sites (CUS) and strong absorption in the UV range, was employed to replace traditional organic matrices, and it was found that MIL-101(Cr) can dramatically eliminate the background peaks, showing high signal-to-noise level in the analysis of small molecules. As proof-of-concept, quercetin, daidzein, genistein and naringenin, members of flavonol family which widely exists in food and natural products, were successfully determined by utilizing MIL-101(Cr) as the surface-assisted matrix, and the detection of quercetin was sensitive with good salt tolerance and reproducibility. Under optimal conditions, the mass peak intensity exhibited good linear relationships in the range from 0.25µg/mL-7.00µg/mL for quercetin (R2=0.996) with detection limit 2.11ng/mL (3σ/k), making the identification of quercetin in sophora japonica successfully. With this strategy we have demonstrated the potentiality of MIL-101(Cr) nanomaterials as MALDI-MS matrix for the detection of small molecules.
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Beibei, Chongqing 400715, China.
authors.forename: Guobin
authors.initials: G
authors.lastname: Han
authors.affiliation: Chongqing Key Laboratory of Biomedical Analysis, Chongqing Science and Technology Commission, College of Pharmaceutical Sciences, Southwest University, Beibei, Chongqing 400715, China.
authors.forename: Qiaoling
authors.initials: Q
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Beibei, Chongqing 400715, China.
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Beibei, Chongqing 400715, China.
authors.forename: Tiantian
authors.initials: T
authors.lastname: Xing
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Beibei, Chongqing 400715, China; Chongqing Key Laboratory of Biomedical Analysis, Chongqing Science and Technology Commission, College of Pharmaceutical Sciences, Southwest University, Beibei, Chongqing 400715, China.
authors.forename: Chengzhi
authors.initials: C
authors.lastname: Huang
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Beibei, Chongqing 400715, China. Electronic address: liyf@swu.edu.cn.
authors.forename: Yuanfang
authors.initials: Y
authors.lastname: Li
mesh_terms: D002138:Calibration; D056831:Coordination Complexes / Q000737:chemistry*; D057230:Limit of Detection; D000073396:Metal-Organic Frameworks; D011794:Quercetin / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012492:Salts / Q000737:chemistry; D029909:Sophora / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D056831:Coordination Complexes; C000589635:MIL-101; D000073396:Metal-Organic Frameworks; D012492:Salts; D011794:Quercetin
keywords: MALDI matrix; MALDI-TOF-MS; Metal-organic frameworks; Quercetin analysis; Sophora japonica