title: Vascular endothelial growth factor-targeted paclitaxel-loaded liposome microbubbles and inhibition of human epidermoid-2 cell proliferation.
abstract: Vascular endothelial growth factor (VEGF) plays an important role in the formation of capillary blood vessels. The purpose of this study was to evaluate the inhibitory effect of VEGF-targeted paclitaxel (PTX)-loaded liposome microbubbles (VTPLLMs) on the proliferation of human epidermoid (Hep-2) laryngeal squamous cell carcinoma (SCC).
  
  
  
  Six groups were randomly divided. The inhibitory effects on Hep-2 proliferation were assessed by a methylthiazol tetrazolium (MTT) method. The cell cycle distributions were investigated by flow cytometry. The expression variations of VEGF and caspase-3 were compared by Western blotting.
  
  
  
  Proliferation of Hep-2 in the VTPLLM+ultrasound group was significantly inhibited, and flow cytometry showed that the Hep-2 cells were significantly blocked at stage G2/M compared with other groups. Moreover, Western blotting showed VEGF expression was downregulated and caspase-3 expression was upregulated.
  
  
  
  VTPLLMs can efficiently inhibit tumor cell proliferation and outperform nontargeted microbubbles or PTX. © 2017 Wiley Periodicals, Inc. Head Neck 39: 656-661, 2017.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Lian
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Luo
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects*; D003124:Colorimetry / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D056945:Hep G2 Cells; D006801:Humans; D007822:Laryngeal Neoplasms / Q000188:drug therapy / Q000473:pathology; D008081:Liposomes / Q000494:pharmacology; D045423:Microbubbles; D058990:Molecular Targeted Therapy; D017239:Paclitaxel / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D042461:Vascular Endothelial Growth Factor A / Q000187:drug effects*
chemical_list: D008081:Liposomes; D042461:Vascular Endothelial Growth Factor A; D017239:Paclitaxel
keywords: human epidermoid carcinoma cells (Hep-2); inhibition effect; targeted therapy; ultrasound microbubbles; vascular endothelial growth factor (VEGF)