title: Comparative Proteomic Analysis of Bleomycin-induced Pulmonary Fibrosis Based on Isobaric Tag for Quantitation.
abstract: BACKGROUND
  Pulmonary fibrosis (PF) is a destructive pulmonary disease and the molecular mechanisms underlying PF are unclear. This study investigated differentially expressed proteins associated with the occurrence and development of PF in rat lung tissue with bleomycin-induced PF.
  
  
  METHODS
  Sixteen Sprague-Dawley rats were randomly divided into 2 groups: the PF model group (n = 8) and the control group (n = 8). After successfully establishing the rat PF model induced by bleomycin, the differentially expressed proteins in the 2 groups were identified through isobaric tag for relative and absolute quantitation coupled with liquid chromatography-mass spectrometry and bioinformatics analysis.
  
  
  RESULTS
  A total of 146 differentially expressed proteins were identified; 88 of which displayed increased abundance and 58 were downregulated in the PF rat model group. Most functional proteins were associated with extracellular matrix, inflammation, damage response, vitamin A synthesis and metabolism. Critical proteins related to PF development and progression was identified, such as type V collagen-3, arachidonic acid 12-lipoxygenase, arachidonic acid 15-lipoxygenase and cytochrome P4501A1. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that these differentially expressed proteins were enriched in extracellular matrix receptor interaction pathway, renin-angiotensin system and metabolic pathway of retinol.
  
  
  CONCLUSIONS
  The proteins expressed in bleomycin-induced PF rat model provide important data for further functional analysis of proteins involved in PF.
authors.affiliation: Department of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, PR China; Department of Urology, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Henan, PR China.
authors.forename: Tiejun
authors.initials: T
authors.lastname: Yang
authors.affiliation: Pharmacy College, Xinxiang Medical University, Xinxiang, Henan, PR China.
authors.forename: Yanlong
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Department of Urology, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Henan, PR China.
authors.forename: Yongkang
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Oncology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Cao
authors.affiliation: Department of Oncology, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Henan, PR China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, PR China. Electronic address: 2542015600@qq.com.
authors.forename: Baoping
authors.initials: B
authors.lastname: Qiao
mesh_terms: D000818:Animals; D001761:Bleomycin; D002853:Chromatography, Liquid; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics / Q000378:metabolism; D006909:Hydroxyproline / Q000378:metabolism; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008297:Male; D040901:Proteomics / Q000379:methods*; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry
chemical_list: D001761:Bleomycin; D019363:Cytochrome P-450 CYP1A1; D006909:Hydroxyproline
keywords: Bleomycin; Isobaric tag for relative and absolute quantitation; Proteome; Pulmonary fibrosis; Rat model