title: Genome Analysis of a Zygomycete Fungus Choanephora cucurbitarum Elucidates Necrotrophic Features Including Bacterial Genes Related to Plant Colonization.
abstract: A zygomycete fungus, Choanephora cucurbitarum is a plant pathogen that causes blossom rot in cucurbits and other plants. Here we report the genome sequence of Choanephora cucurbitarum KUS-F28377 isolated from squash. The assembled genome has a size of 29.1 Mbp and 11,977 protein-coding genes. The genome analysis indicated that C. cucurbitarum may employ a plant pathogenic mechanism similar to that of bacterial plant pathogens. The genome contained 11 genes with a Streptomyces subtilisin inhibitor-like domain, which plays an important role in the defense against plant immunity. This domain has been found only in bacterial genomes. Carbohydrate active enzyme analysis detected 312 CAZymes in this genome where carbohydrate esterase family 6, rarely found in dikaryotic fungal genomes, was comparatively enriched. The comparative genome analysis showed that the genes related to sexual communication such as the biosynthesis of β-carotene and trisporic acid were conserved and diverged during the evolution of zygomycete genomes. Overall, these findings will help us to understand how zygomycetes are associated with plants.
authors.affiliation: Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea.
authors.forename: Byoungnam
authors.initials: B
authors.lastname: Min
authors.affiliation: Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea.
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea.
authors.forename: Hongjae
authors.initials: H
authors.lastname: Park
authors.affiliation: Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea.
authors.forename: Hyeon-Dong
authors.initials: HD
authors.lastname: Shin
authors.affiliation: Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea.
authors.forename: In-Geol
authors.initials: IG
authors.lastname: Choi
mesh_terms: D000595:Amino Acid Sequence; D015169:Colony Count, Microbial; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics; D005658:Fungi / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D005800:Genes, Fungal; D016681:Genome, Fungal; D054884:Host-Pathogen Interactions / Q000235:genetics; D058977:Molecular Sequence Annotation; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D000072417:Protein Domains; D016415:Sequence Alignment; D014774:Virulence / Q000235:genetics
chemical_list: D005656:Fungal Proteins