title: Potential Osteoinductive Effects of Calcitriol on the m-RNA of Mesenchymal Stem Cells Derived from Human Alveolar Periosteum.
abstract: This study characterized alveolar periosteum-derived mesenchymal stem cells (P-MSCs) and examined the hypothesis that 1,25-(OH)2D3(calcitriol) exerts osteoinductive effects on P-MSCs. The mRNA expressions of alkaline phosphatase (ALP), bone sialoprotein (BSP), core-binding factor alpha-1 (CBFA1), collagen-1 (Col-1), osteocalcin (OCN), and vitamin D3receptor (VDR) were assessed after incubation with calcitriol for 2 weeks. Vitamin C as positive control (Vit. C-p) increased ALP and CBFA1 mRNA expression at both 1 and 2 weeks and increased BSP and Col-1 mRNA expression only at the first week. A concentration of 10-8M calcitriol enhanced ALP, CBFA1, Col-1, and OCN mRNA expression at both weeks and BSP mRNA expression at the first week. Furthermore, 10-7M calcitriol increased the mRNA expressions of all compounds at both weeks, except that of CBFA1 at the first week. 10-8M calcitriol and Vit. C-p enhanced ALP activity at the second and third weeks. The results revealed that 10-9, 10-8, and 10-7M calcitriol induced osteoinduction in alveolar P-MSCs by increasing ALP, CBFA1, Col-1, and OCN mRNA expression. A 10-7M calcitriol yielded a higher mRNA expression than Vit. Cp on VDR and OCN mRNA expression at both weeks and on Col-1 mRNA at the second week.
authors.affiliation: Department of Periodontics, Chang Gung Memorial Hospital, Linkou, Taiwan; Chang Gung University, Taoyuan, Taiwan; School of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
authors.forename: Hsiang-Hsi
authors.initials: HH
authors.lastname: Hong
authors.affiliation: California Northstate University, College of Medicine, Elk Grove, CA, USA.
authors.forename: Adrienne
authors.initials: A
authors.lastname: Hong
authors.affiliation: Chang Gung University, Taoyuan, Taiwan; Division of Clinical Toxicology, Department of Nephrology, Chang Gung Memorial Hospital, Linkou, Taiwan; Kidney Research Center, Chang Gung Memorial Hospital, Linkou, Taiwan; Center for Tissue Engineering, Chang Gung Memorial Hospital, Linkou, Taiwan.
authors.forename: Tzung-Hai
authors.identifier: 0000-0002-0907-1505
authors.initials: TH
authors.lastname: Yen
authors.affiliation: Chang Gung University, Taoyuan, Taiwan; Department of Periodontics, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
authors.forename: Yen-Li
authors.identifier: 0000-0002-6213-7497
authors.initials: YL
authors.lastname: Wang
mesh_terms: D000328:Adult; D002117:Calcitriol / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D008875:Middle Aged; D010521:Periosteum / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*
chemical_list: D012333:RNA, Messenger; D002117:Calcitriol