title: Resource Reallocation in Bacteria by Reengineering the Gene Expression Machinery.
abstract: Bacteria have evolved complex regulatory networks to control the activity of transcription and translation, and thus the growth rate, over a range of environmental conditions. Reengineering RNA polymerase and ribosomes allows modifying naturally evolved regulatory networks and thereby profoundly reorganizing the manner in which bacteria allocate resources to different cellular functions. This opens new opportunities for our fundamental understanding of microbial physiology and for a variety of applications. Recent breakthroughs in genome engineering and the miniaturization and automation of culturing methods have offered new perspectives for the reengineering of the transcription and translation machinery in bacteria as well as the development of novel in vitro and in vivo gene expression systems. We review different examples from the unifying perspective of resource reallocation, and discuss the impact of these approaches for microbial systems biology and biotechnological applications.
authors.affiliation: Inria, Grenoble Rhône-Alpes Research Centre, Montbonnot, Saint Ismier Cedex, France. Electronic address: hidde.de-jong@inria.fr.
authors.forename: Hidde
authors.initials: H
authors.lastname: de Jong
authors.affiliation: Inria, Grenoble Rhône-Alpes Research Centre, Montbonnot, Saint Ismier Cedex, France; Université Grenoble Alpes, Laboratoire Interdisciplinaire de Physique (CNRS UMR 5588), Saint Martin d'Hères, France. Electronic address: hans.geiselmann@univ-grenoble-alpes.fr.
authors.forename: Johannes
authors.initials: J
authors.lastname: Geiselmann
authors.affiliation: Université Grenoble Alpes, Laboratoire Interdisciplinaire de Physique (CNRS UMR 5588), Saint Martin d'Hères, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Ropers
mesh_terms: D001419:Bacteria / Q000235:genetics* / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D053263:Gene Regulatory Networks / Q000235:genetics*; D005818:Genetic Engineering; D016680:Genome, Bacterial; D012313:RNA / Q000235:genetics / Q000378:metabolism; D040841:Resource Allocation; D012270:Ribosomes / Q000235:genetics; D058615:Synthetic Biology; D049490:Systems Biology
chemical_list: D012313:RNA; D012321:DNA-Directed RNA Polymerases
keywords: Biotechnology; Genome engineering; RNA polymerase; Resource allocation; Ribosome; Synthetic biology; Systems biology