title: Characterization and carboplatin loaded chitosan nanoparticles for the chemotherapy against breast cancer in vitro studies.
abstract: Aim of the studies to synthesized chitosan nanoparticles by an ionic interaction procedure. The nanoparticles were characterized by physicochemical methods like, DLS, TEM, Surface potential measurements, FT-IR and DSC. The average particle size of chitosan and carboplatin nanoparticles was found to be 277.25±11.37nm and 289.30±8.15nm and zeta potential was found to be 31±3.14mV and 33±2.15mV respectively with low polydispersity index. The maximum entrapment of carboplatin in nanoparticles was a spherical shape with a positive charge. The maximum encapsulation and loading efficiencies of carboplatin (5mg/ml) were obtained to be 58.43% and 13.27% respectively. The nanocarboplatin was better blood compatibility as compared to chitosan nanoparticles. Finally, the cytotoxic effects of the carboplatin loaded chitosan nanoparticles were tested in-vitro against breast cancer (MCF-7) cell lines. Our studies showed that the chitosan nanoparticles could be used as a promising candidate for drug delivery for the therapeutic treatment of breast cancer.
authors.affiliation: Genome Biology Lab, Department of Biosciences, Jamia Millia Islamia, New Delhi, 110025, India. Electronic address: asad1amu@gmail.com.
authors.forename: Md Asad
authors.initials: MA
authors.lastname: Khan
authors.affiliation: Genome Biology Lab, Department of Biosciences, Jamia Millia Islamia, New Delhi, 110025, India.
authors.forename: Md
authors.initials: M
authors.lastname: Zafaryab
authors.affiliation: Genome Biology Lab, Department of Biosciences, Jamia Millia Islamia, New Delhi, 110025, India.
authors.forename: Syed Hassan
authors.initials: SH
authors.lastname: Mehdi
authors.affiliation: Department of Anatomy, All India Institute of Medical Sciences, New Delhi, 110029, India.
authors.forename: Javed
authors.initials: J
authors.lastname: Quadri
authors.affiliation: Genome Biology Lab, Department of Biosciences, Jamia Millia Islamia, New Delhi, 110025, India. Electronic address: rizvi_ma@yahoo.com.
authors.forename: M Moshahid A
authors.initials: MM
authors.lastname: Rizvi
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D001692:Biological Transport; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D016190:Carboplatin / Q000737:chemistry* / Q000494:pharmacology*; D048271:Chitosan / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D065546:Drug Liberation; D004355:Drug Stability; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D061986:MCF-7 Cells; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D048271:Chitosan; D016190:Carboplatin
keywords: Anticancer; Carboplatin; Chitosan; Nanocarboplatin; Nanoparticles