title: Elucidation of stress-induced degradation products of mangiferin: Method development and validation.
abstract: The degradation behavior of mangiferin, under various ICH Q1A(R2) recommended stress conditions, was studied using an isocratic elution with mobile phase (pH 2.4), composed of acetonitrile and 1% orthophosphoric acid (12:88 v/v) at a flow rate of 1.0 mL/min, with λmax262 nm. It was suitably adapted for LC-MS studies by replacing with 1% acetic acid (ACN-1% acetic acid; 18:82) and the pH was adjusted to 3.0. Extensive degradation was found to occur during alkaline medium stress studies at 2.31 min of retention time at λmaxof 235 nm. The mass spectrum of mangiferin, 3 h after treatment with 0.1 M NaOH, clearly shows the rupture of the tricyclic ring, indicating that a fragment at m/z - 269 was formed. Furthermore, the results were supported by nuclear magnetic resonance as well. However, no degradation was observed in other stress conditions.
authors.affiliation: University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh, India.
authors.forename: Rajneet Kaur
authors.initials: RK
authors.lastname: Khurana
authors.affiliation: GHG Khalsa College of Pharmacy, Gurusar Sadhar, India.
authors.forename: Satvinder
authors.initials: S
authors.lastname: Kaur
authors.affiliation: DAV College, Chandigarh, India.
authors.forename: Jasleen
authors.initials: J
authors.lastname: Kaur
authors.affiliation: University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh, India.|UGC Centre of Excellence in Applications of Nanomaterials, Nanoparticles and Nanocomposites (Biomedical Sciences), Panjab University, Chandigarh, India.
authors.forename: Bhupinder
authors.identifier: http://orcid.org/0000-0003-0340-5524
authors.initials: B
authors.lastname: Singh
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry*; D002853:Chromatography, Liquid; D004355:Drug Stability; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D031022:Mangifera / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D053719:Tandem Mass Spectrometry; D044004:Xanthones / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; C501277:Mangifera indica extract; D010936:Plant Extracts; D044004:Xanthones; C013592:mangiferin
keywords: LC-MS-MS; degaradation products; mass spectra; xanthone ring