title: Toxicity and Larvicidal Activity of Podophyllum-Based Lignans Against Aedes aegypti (Diptera: Culicidae).
abstract: Aedes aegypti L. (Diptera: Culicidae) is a mosquito species that has adapted to urban environments and is the main vector of dengue viruses. Because of the increasing incidence of dengue, a more environmentally acceptable insecticide needs to be found. Natural products have been and continue to be an important source of leading compounds that can be modified in order to develop new drugs. The lignan family of natural products includes compounds with a diverse spectrum of biological activity. Podophyllotoxin and its related lignans represent an exciting class of natural products that can be targeted at different types of biological activity and are therefore worth exploring further. This study had the aim of evaluating the larvicidal activity of an ethanolic extract from the rhizomes and roots of Podophyllum hexandrum (PM-3) and its isolated lignans, podophyllotoxone (1) and desoxypodophyllotoxin (2), on the larvae of the mosquito vector Ae. aegypti. The PM-3 extract and the compounds (1) and (2) were dissolved in a mixture of acetone and dimethylsulfoxide at final concentrations of 1, 10, 30, 50, 100, and 200 μg/ml. After dilution, the solutions were applied (μg/ml) to the larvae-rearing medium. Overall, the ethanolic extract from the rhizomes and roots of P. hexandrum and the compounds (1) and (2) showed larvicidal activity against the larvae of Ae. aegypti According to the results from this study, it can be concluded that podophyllotoxone (1) and desoxypodophyllotoxin (2) exhibited significant toxicity toward Ae. aegypti larvae.
authors.affiliation: Laboratório de Insetos Vetores, Universidade Severino Sombra, Rua Antenor Caravana, 677, Barreiro, 27700-000 Vassouras, RJ, Brazil (marise.maleck@gmail.com; priscilahollanda@yahoo.com.br; mserdeiro@gmail.com) marise.maleck@gmail.com.|Pró-Reitoria das Ciências da Saúde e Humanas, Universidade Severino Sombra, Av. Expedicionário Oswaldo Almeida Ramos, 280, 27700-000 Vassouras, RJ, Brazil.|Mestrado Profissional em Ciências Ambientais, Universidade Severino Sombra, Av. Expedicionário Oswaldo Almeida Ramos, 280, 27700-000 Vassouras, RJ, Brazil.
authors.forename: Marise
authors.initials: M
authors.lastname: Maleck
authors.affiliation: Laboratório de Insetos Vetores, Universidade Severino Sombra, Rua Antenor Caravana, 677, Barreiro, 27700-000 Vassouras, RJ, Brazil (marise.maleck@gmail.com; priscilahollanda@yahoo.com.br; mserdeiro@gmail.com).|Pró-Reitoria das Ciências da Saúde e Humanas, Universidade Severino Sombra, Av. Expedicionário Oswaldo Almeida Ramos, 280, 27700-000 Vassouras, RJ, Brazil.
authors.forename: Priscila de Oliveira
authors.initials: PO
authors.lastname: Hollanda
authors.affiliation: Laboratório de Insetos Vetores, Universidade Severino Sombra, Rua Antenor Caravana, 677, Barreiro, 27700-000 Vassouras, RJ, Brazil (marise.maleck@gmail.com; priscilahollanda@yahoo.com.br; mserdeiro@gmail.com).|Mestrado Profissional em Ciências Ambientais, Universidade Severino Sombra, Av. Expedicionário Oswaldo Almeida Ramos, 280, 27700-000 Vassouras, RJ, Brazil.
authors.forename: Michele Teixeira
authors.initials: MT
authors.lastname: Serdeiro
authors.affiliation: Laboratório de Biologia Molecular e Doenças Endêmicas, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brazil, 4365, 21045-900 Rio de Janeiro, RJ, Brazil (soaresroa@gmail.com).
authors.forename: Renata Oliveira de Araújo
authors.initials: RO
authors.lastname: Soares
authors.affiliation: Laboratório de Transmissores de Hematozoários e Núcleo Operacional Sentinela de Mosquitos Vetores, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brazil, 4365, 21045-900 Rio de Janeiro, RJ, Brazil (nahonorio1@gmail.com).
authors.forename: Nildimar Alves
authors.initials: NA
authors.lastname: Honório
authors.affiliation: Serviço de Biotecnologia Vegetal, Fundação Ezequiel Dias, Rua Conde Pereira Carneiro, 80, 30510-010 Belo Horizonte, MG, Brazil (claudia.gontijo@funed.mg.gov.br).
authors.forename: Cláudia Gontijo
authors.initials: CG
authors.lastname: Silva
mesh_terms: D000330:Aedes / Q000254:growth & development; D000818:Animals; D007306:Insecticides; D007814:Larva / Q000254:growth & development; D017705:Lignans; D009032:Mosquito Control / Q000379:methods; D010936:Plant Extracts; D018517:Plant Roots / Q000737:chemistry; D011035:Podophyllum / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry
chemical_list: D007306:Insecticides; D017705:Lignans; D010936:Plant Extracts
keywords: Aedes aegypti; Podophyllum hexandrum; larvicidal activity; lignans