title: Global identification of microRNAs associated with chlorantraniliprole resistance in diamondback moth Plutella xylostella (L.).
abstract: The diamondback moth (DBM), Plutella xylostella (L.), is one of the most serious cruciferous pests and has developed high resistance to most insecticides, including chlorantraniliprole. Previous studies have reported several protein-coding genes that involved in chlorantraniliprole resistance, but research on resistance mechanisms at the post-transcription level is still limited. In this study, a global screen of microRNAs (miRNAs) associated with chlorantraniliprole resistance in P. xylostella was performed. The small RNA libraries for a susceptible (CHS) and two chlorantraniliprole resistant strains (CHR, ZZ) were constructed and sequenced, and a total of 199 known and 30 novel miRNAs were identified. Among them, 23 miRNAs were differentially expressed between CHR and CHS, and 90 miRNAs were differentially expressed between ZZ and CHS, of which 11 differentially expressed miRNAs were identified in both CHR and ZZ. Using miRanda and RNAhybrid, a total of 1,411 target mRNAs from 102 differentially expressed miRNAs were predicted, including mRNAs in several groups of detoxification enzymes. The expression of several differentially expressed miRNAs and their potential targets was validated by qRT-PCR. The results may provide important clues for further study of the mechanisms of miRNA-mediated chlorantraniliprole resistance in DBM and other target insects.
authors.affiliation: Department of Entomology, China Agricultural University, Beijing, 100193, P. R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Department of Entomology, China Agricultural University, Beijing, 100193, P. R. China.
authors.forename: Xiuxia
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Entomology, China Agricultural University, Beijing, 100193, P. R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Entomology, China Agricultural University, Beijing, 100193, P. R. China.
authors.forename: Xiwu
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Entomology, China Agricultural University, Beijing, 100193, P. R. China.
authors.forename: Pei
authors.initials: P
authors.lastname: Liang
mesh_terms: D000818:Animals; D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D059014:High-Throughput Nucleotide Sequencing; D007305:Insecticide Resistance / Q000235:genetics*; D007306:Insecticides / Q000494:pharmacology*; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D058977:Molecular Sequence Annotation; D009036:Moths / Q000145:classification / Q000187:drug effects* / Q000235:genetics*; D009690:Nucleic Acid Conformation; D034622:RNA Interference; D015203:Reproducibility of Results; D059467:Transcriptome; D062367:ortho-Aminobenzoates / Q000494:pharmacology*
chemical_list: D007306:Insecticides; D035683:MicroRNAs; D062367:ortho-Aminobenzoates; C517733:chlorantranilipole