title: Comprehensive assessment of peripheral blood TCRβ repertoire in infectious mononucleosis and chronic active EBV infection patients.
abstract: Epstein-Barr virus (EBV) primary infection is usually asymptomatic, but it sometimes progresses to infectious mononucleosis (IM). Occasionally, some people develop chronic active EBV infection (CAEBV) with underlying immunodeficiency, which belongs to a continuous spectrum of EBV-associated lymphoproliferative disorders (EBV+LPD) with heterogeneous clinical presentations and high mortality. It has been well established that T cell-mediated immune response plays a critical role in the disease evolution of EBV infection. Recently, high-throughput sequencing of the hypervariable complementarity-determining region 3 (CDR3) segments of the T cell receptor (T cell receptor β (TCRβ)) has emerged as a sensitive approach to assess the T cell repertoire. In this study, we fully characterized the diversity of peripheral blood TCRβ repertoire in IM (n = 6) and CAEBV patients (n = 5) and EBV-seropositive controls (n = 5). Compared with the healthy EBV-seropositive controls, both IM and CAEBV patients demonstrate a significant decrease in peripheral blood TCRβ repertoire diversity, basically, including narrowed repertoire breadth, highly expanded clones, and skewed CDR3 length distribution. However, there is no significant difference between IM and CAEBV patients. Furthermore, we observed some disease-related preferences in TRBV/TRBJ usage and combinations, as well as lots of T cell clones shared by different groups (unique or overlapped) involved in public T cell responses, which provide more detailed insights into the divergent disease evolution.
authors.affiliation: Biomedical Research Institute, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong Province, 518036, China.
authors.forename: Shenglin
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Hematology, Peking University Shenzhen Hospital, Shenzhen, Guangdong, 518036, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Changyi People's Hospital, Changyi, Shandong, 261300, China.
authors.forename: Dongli
authors.initials: D
authors.lastname: Huang
authors.affiliation: Department of Hematology, Peking University Shenzhen Hospital, Shenzhen, Guangdong, 518036, China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Hematology, Peking University Shenzhen Hospital, Shenzhen, Guangdong, 518036, China.
authors.forename: Fengluan
authors.initials: F
authors.lastname: Zhong
authors.affiliation: Department of Hematology, Peking University Shenzhen Hospital, Shenzhen, Guangdong, 518036, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Feng
authors.affiliation: Department of Hematology, Peking University Shenzhen Hospital, Shenzhen, Guangdong, 518036, China.
authors.forename: Xueru
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Hematology, Peking University Shenzhen Hospital, Shenzhen, Guangdong, 518036, China.
authors.forename: Qingxiang
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Biomedical Research Institute, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong Province, 518036, China.
authors.forename: Xiaofan
authors.initials: X
authors.lastname: Chen
authors.affiliation: Biomedical Research Institute, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong Province, 518036, China. zhangweispace@yeah.net.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Hematology, Peking University Shenzhen Hospital, Shenzhen, Guangdong, 518036, China. zyiqu@163.com.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000097:blood; D002908:Chronic Disease; D020031:Epstein-Barr Virus Infections / Q000097:blood* / Q000175:diagnosis* / Q000453:epidemiology; D005260:Female; D006801:Humans; D007244:Infectious Mononucleosis / Q000097:blood* / Q000175:diagnosis* / Q000453:epidemiology; D008297:Male; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000097:blood*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D016693:Receptors, Antigen, T-Cell, alpha-beta
keywords: Chronic active EBV infection (CAEBV); Epstein-Barr virus (EBV); Infectious mononucleosis (IM); T cell receptor (TCRβ) repertoire complementarity-determining region 3 (CDR3)