title: Seminal miRNA Relationship with Apoptotic Markers and Oxidative Stress in Infertile Men with Varicocele.
abstract: Aim. This study aimed to assess seminal miRNA relationship with seminal apoptotic markers and oxidative stress (OS) in infertile men associated with varicocele (Vx).Methods. In all, 220 subjects were divided into the following groups: fertile normozoospermic men, fertile normozoospermic men with Vx, infertile oligoasthenoteratozoospermic (OAT) men without Vx, and infertile OAT men with Vx. They were subjected to history taking, clinical examination, and semen analysis. In their semen, the following were estimated: miRNA-122, miRNA-181a, and miRNA-34c5 using quantitative real-time PCR, apoptotic markers (BAX, BCL2) protein expression, and OS markers [malondialdehyde (MDA) and glutathione peroxidase (GPx)].Results. The mean levels of seminal miRNA-122, miRNA-181a, and miRNA-34c5 were significantly reduced in infertile OAT men with Vx compared with other groups coupled with Vx grade and Vx bilaterality. Seminal miRNA-122, miRNA-181a, and miRNA-34c5 were positively correlated with sperm concentration, total sperm motility, sperm normal morphology, seminal GPx, and seminal BCL2 and negatively correlated with seminal MDA and seminal BAX.Conclusions. Seminal miRNA-122, miRNA-181a, and miRNA-34c5 are decreased in infertile OAT men with Vx associated with increased Vx grade and Vx bilaterality. In addition, they are positively correlated with sperm parameters and negatively correlated with OS, apoptotic markers.
authors.affiliation: Department of Andrology & Sexology, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors.forename: Taymour
authors.identifier: 0000-0003-3627-0662
authors.initials: T
authors.lastname: Mostafa
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors.forename: Laila A
authors.initials: LA
authors.lastname: Rashed
authors.affiliation: Department of Andrology and Sexology, Faculty of Medicine, Beni Suef University, Beni Suef, Egypt.
authors.forename: Nashaat I
authors.initials: NI
authors.lastname: Nabil
authors.affiliation: Department of Andrology & Sexology, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors.forename: Ihab
authors.initials: I
authors.lastname: Osman
authors.affiliation: Department of Andrology and Sexology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
authors.forename: Rashad
authors.initials: R
authors.lastname: Mostafa
authors.affiliation: Department of Andrology and Sexology, Faculty of Medicine, Beni Suef University, Beni Suef, Egypt.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Farag
mesh_terms: D000328:Adult; D017209:Apoptosis; D015415:Biomarkers / Q000378:metabolism; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D018384:Oxidative Stress; D012661:Semen / Q000378:metabolism*; D014646:Varicocele / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D035683:MicroRNAs