title: Melting Behavior of Zipper-Structured Lipopeptides in Lipid Bilayer.
abstract: A zipper-structured lipopeptide is expected to play a role of "intelligent valve" in the lipid bilayer. In this paper, a series of zipper-structured lipopeptides have been designed for preparing thermocontrollable hybrid liposomes. Their conformational transition as a function of temperature in lipid bilayer has been investigated for understanding the influences of molecular structure and bilayer property on biofunction. The melting temperatures Tmof the lipopeptides have been found to depend on their molecular structures. When the lipopeptides have been doped in bilayer, an increase of size of alkyl chain increases the stability of the α-helix resulting in a decrease in fluidity of lipid bilayer. However, an increase of amino groups at N-terminal is found to decrease the stability of the spatial structure. The thermocontrollability of the "valve" in lipid bilayer is confirmed by drug release experiments under different temperatures. Meanwhile, effects of bilayer properties on the thermosensitivity of lipopeptides have also been investigated. Results show the Tmof lipopeptide doped in bilayer decreases with the increase of membrane fluidity. Furthermore, the reversibility of the thermocontrolled "valve" is also proven by release drug under intermittent temperatures. It could be concluded that the molecular structure of the lipopeptide, as well as the property of bilayer, give great influence on the biofunction of the hybrid liposomes.
authors.affiliation: Key Laboratory for Advanced Materials and School of Chemistry and Molecular Engineering, East China University of Science and Technology , Shanghai, 200237, China.
authors.forename: Sijia
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory for Advanced Materials and School of Chemistry and Molecular Engineering, East China University of Science and Technology , Shanghai, 200237, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Han
authors.affiliation: Key Laboratory for Advanced Materials and School of Chemistry and Molecular Engineering, East China University of Science and Technology , Shanghai, 200237, China.
authors.forename: Danyang
authors.initials: D
authors.lastname: Liu
authors.affiliation: Key Laboratory for Advanced Materials and School of Chemistry and Molecular Engineering, East China University of Science and Technology , Shanghai, 200237, China.
authors.forename: Mengya
authors.initials: M
authors.lastname: Li
authors.affiliation: Key Laboratory for Advanced Materials and School of Chemistry and Molecular Engineering, East China University of Science and Technology , Shanghai, 200237, China.
authors.forename: Shouhong
authors.identifier: 0000-0002-6019-0585
authors.initials: S
authors.lastname: Xu
authors.affiliation: Key Laboratory for Advanced Materials and School of Chemistry and Molecular Engineering, East China University of Science and Technology , Shanghai, 200237, China.
authors.forename: Honglai
authors.initials: H
authors.lastname: Liu
mesh_terms: D002784:Cholesterol / Q000031:analogs & derivatives / Q000737:chemistry; D004317:Doxorubicin / Q000737:chemistry; D065546:Drug Liberation; D016350:Leucine Zippers; D008051:Lipid Bilayers / Q000737:chemistry*; D055666:Lipopeptides / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008560:Membrane Fluidity; D015394:Molecular Structure; D010713:Phosphatidylcholines / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry; D011487:Protein Conformation; D013501:Surface-Active Agents / Q000737:chemistry; D044366:Transition Temperature
chemical_list: D008051:Lipid Bilayers; D055666:Lipopeptides; D008081:Liposomes; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D013501:Surface-Active Agents; C070648:3-(N-(N',N'-dimethylaminoethane)carbamoyl)cholesterol; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine; D004317:Doxorubicin; D002784:Cholesterol; C010942:1,2-distearoyllecithin