title: Transcriptome Profiling of the Lungs Reveals Molecular Clock Genes Expression Changes after Chronic Exposure to Ambient Air Particles.
abstract: The symptoms of asthma, breathlessness, insomnia, etc. all have relevance to pulmonary rhythmic disturbances. Epidemiology and toxicology studies have demonstrated that exposure to ambient air particles can result in pulmonary dysfunction. However, there are no data directly supporting a link between air pollution and circadian rhythm disorder. In the present study, we found that breathing highly polluted air resulted in changes of the molecular clock genes expression in lung by transcriptome profiling analyses in a rodent model. Compared to those exposed to filtered air, in both pregnant and offspring rats in the unfiltered group, key clock genes (Per1,Per2,Per3,Rev-erbαandDbp) expression level decreased andBmal1expression level increased. In both rat dams and their offspring, after continuous exposure to unfiltered air, we observed significant histologic evidence for both perivascular and peribronchial inflammation, increased tissue and systemic oxidative stress in the lungs. Our results suggest that chronic exposure to particulate matter can induce alterations of clock genes expression, which could be another important pathway for explaining the feedbacks of ambient particle exposure in addition to oxidative stress and inflammation.
authors.affiliation: College of Environmental Science and Engineering, Dong Hua University, Shanghai 201620, China. penches@163.com.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Song
authors.affiliation: Laboratory of Environmental Criteria and Risk Assessment & Environmental Standards Institute, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. lizg@craes.org.cn.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Laboratory of Environmental Criteria and Risk Assessment & Environmental Standards Institute, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. lixiaoqian@craes.org.cn.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Li
authors.affiliation: Laboratory of Environmental Criteria and Risk Assessment & Environmental Standards Institute, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. yanglx@craes.org.cn.
authors.forename: Lixin
authors.initials: L
authors.lastname: Yang
authors.affiliation: Laboratory of Environmental Criteria and Risk Assessment & Environmental Standards Institute, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. zhang_lulu@yeah.net.
authors.forename: Lulu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Laboratory of Environmental Criteria and Risk Assessment & Environmental Standards Institute, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. linannqinian@163.com.
authors.forename: Nannan
authors.initials: N
authors.lastname: Li
authors.affiliation: Laboratory of Environmental Criteria and Risk Assessment & Environmental Standards Institute, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. chenpreteen@sina.com.
authors.forename: Chen
authors.initials: C
authors.lastname: Guo
authors.affiliation: College of Environmental Science and Engineering, Dong Hua University, Shanghai 201620, China. sisrlu@126.com.
authors.forename: Shuyu
authors.initials: S
authors.lastname: Lu
authors.affiliation: Laboratory of Environmental Criteria and Risk Assessment & Environmental Standards Institute, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. weiyj@craes.org.cn.
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Wei
mesh_terms: D000397:Air Pollution / Q000009:adverse effects*; D000818:Animals; D002940:Circadian Rhythm / Q000187:drug effects; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D007249:Inflammation / Q000209:etiology* / Q000235:genetics*; D008171:Lung Diseases / Q000209:etiology* / Q000235:genetics* / Q000503:physiopathology; D008297:Male; D052638:Particulate Matter / Q000009:adverse effects*; D011247:Pregnancy; D051381:Rats
chemical_list: D052638:Particulate Matter
keywords: ambient air particles; clock genes; lung; rhythm; transcriptome