title: Transcriptome Analysis of theCf-12-Mediated Resistance Response toCladosporium fulvumin Tomato.
abstract: Cf-12is an effective gene for resisting tomato leaf mold disease caused byCladosporium fulvum(C. fulvum). Unlike many otherCfgenes such asCf-2, Cf-4, Cf-5, andCf-9, no physiological races ofC. fulvumthat are virulent toCf-12carrying plant lines have been identified. In order to better understand the molecular mechanism ofCf-12gene resistance response, RNA-Seq was used to analyze the transcriptome changes at three different stages ofC. fulvuminfection (0, 4, and 8 days post infection [dpi]). A total of 9100 differentially expressed genes (DEGs) between 4 and 0 dpi, 8643 DEGs between 8 and 0 dpi and 2547 DEGs between 8 and 4 dpi were identified. In addition, we found that 736 DEGs shared among the above three groups, suggesting the presence of a common core of DEGs in response toC. fulvuminfection. These DEGs were significantly enriched in defense-signaling pathways such as the calcium dependent protein kinases pathway and the jasmonic acid signaling pathway. Additionally, we found that many transcription factor genes were among the DEGs, indicating that transcription factors play an important role inC. fulvumdefense response. Our study provides new insight on the molecular mechanism ofCfresistance toC. fulvum, especially the unique features ofCf-12in responding toC. fulvuminfection.
authors.affiliation: College of Horticulture, Northeast Agricultural University Harbin, China.
authors.forename: Dong-Qi
authors.initials: DQ
authors.lastname: Xue
authors.affiliation: College of Horticulture, Northeast Agricultural University Harbin, China.
authors.forename: Xiu-Ling
authors.initials: XL
authors.lastname: Chen
authors.affiliation: College of Horticulture, Northeast Agricultural University Harbin, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Life Science, Northeast Agricultural University Harbin, China.
authors.forename: Xin-Feng
authors.initials: XF
authors.lastname: Chai
authors.affiliation: College of Horticulture, Northeast Agricultural University Harbin, China.
authors.forename: Jing-Bin
authors.initials: JB
authors.lastname: Jiang
authors.affiliation: College of Horticulture, Northeast Agricultural University Harbin, China.
authors.forename: Xiang-Yang
authors.initials: XY
authors.lastname: Xu
authors.affiliation: College of Horticulture, Northeast Agricultural University Harbin, China.
authors.forename: Jing-Fu
authors.initials: JF
authors.lastname: Li
keywords: Cf-12 tomato; Cladosporium fulvum; RNA-Seq; differentially expressed genes; resistance response