title: Class II MHC-independent suppressive adhesion of dendritic cells by regulatory T cells in vivo.
abstract: Regulatory T (T reg) cells are essential for peripheral homeostasis and known to target and suppress dendritic cells (DCs). One important mechanism is through prolonged interaction between antigen-specific T reg cells and DCs that down-regulates the co-stimulatory capacity of DCs. However, the dynamics and TCR specificities of such T reg cell-DC interaction and its relevance to the suppressive outcomes for individual DCs have not been clarified. To gain insights into the underlying cellular events in vivo, we analyzed individual T reg cell-DC interaction events in lymph nodes by intravital microscopy. Our results show that, upon exposure to interleukin-2, T reg cells formed prolonged adhesive contact with DCs, independent of antigen or MHC recognition, which significantly suppressed the contemporaneous interaction of the same DCs with antigen-specific conventional T cells and impaired T cell priming. Therefore, T reg cells may function in part as feedback regulators in inflammatory milieu, by suppressing local DCs and interrupting immune activation in a contact-dependent and class II MHC-independent manner.
authors.affiliation: Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 10084, China.|School of Life Sciences, Tsinghua University, Beijing 10084, China.|Institute for Immunology, Tsinghua University, Beijing 10084, China.
authors.forename: Jiacong
authors.initials: J
authors.lastname: Yan
authors.affiliation: Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 10084, China.|Institute for Immunology, Tsinghua University, Beijing 10084, China.|Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing 10084, China.
authors.forename: Bo
authors.identifier: 0000-0002-9596-7307
authors.initials: B
authors.lastname: Liu
authors.affiliation: Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 10084, China.|Institute for Immunology, Tsinghua University, Beijing 10084, China.|Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing 10084, China.|Department of Microbiology, Immunology, and Infectious Diseases, University of Calgary, Calgary, Alberta T2N 1N4, Canada.|Snyder Institute, University of Calgary, Calgary, Alberta T2N 1N4, Canada.
authors.forename: Yan
authors.identifier: 0000-0002-6715-7681
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 10084, China qihai@tsinghua.edu.cn.|School of Life Sciences, Tsinghua University, Beijing 10084, China.|Institute for Immunology, Tsinghua University, Beijing 10084, China.|Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing 10084, China.
authors.forename: Hai
authors.identifier: 0000-0001-5475-3989
authors.initials: H
authors.lastname: Qi
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002450:Cell Communication; D003713:Dendritic Cells / Q000166:cytology / Q000502:physiology*; D000949:Histocompatibility Antigens Class II / Q000502:physiology*; D007376:Interleukin-2 / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D050378:T-Lymphocytes, Regulatory / Q000502:physiology*
chemical_list: D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2