title: Cometabolic degradation of chloramphenicol via a meta-cleavage pathway in a microbial fuel cell and its microbial community.
abstract: The performance of a microbial fuel cell (MFC) in terms of degradation of chloramphenicol (CAP) was investigated. Approximately 84% of 50mg/L CAP was degraded within 12h in the MFC. A significant interaction of pH, temperature, and initial CAP concentration was found on removal of CAP, and a maximum degradation rate of 96.53% could theoretically be achieved at 31.48°C, a pH of 7.12, and an initial CAP concentration of 106.37mg/L. Moreover, CAP was further degraded through a ring-cleavage pathway. The antibacterial activity of CAP towards Escherichia coli ATCC 25922 and Shewanella oneidensis MR-1 was largely eliminated by MFC treatment. High-throughput sequencing analysis indicated that Azonexus, Comamonas, Nitrososphaera, Chryseobacterium, Azoarcus, Rhodococcus, and Dysgonomonas were the predominant genera in the MFC anode biofilm. In conclusion, the MFC shows potential for the treatment of antibiotic residue-containing wastewater due to its high rates of CAP removal and energy recovery.
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Science, Chengdu 610041, China; College of Life Sciences, Sichuan University, Chengdu 610064, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Science, Chengdu 610041, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Science, Chengdu 610041, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: lidp@cib.ac.cn.
authors.forename: Daping
authors.initials: D
authors.lastname: Li
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry; D001673:Biodegradation, Environmental; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D018441:Biofilms; D002701:Chloramphenicol / Q000378:metabolism* / Q000494:pharmacology; D004566:Electrodes / Q000382:microbiology; D004926:Escherichia coli / Q000187:drug effects; D059014:High-Throughput Nucleotide Sequencing; D006863:Hydrogen-Ion Concentration; D059013:Microbial Consortia / Q000235:genetics / Q000502:physiology*; D020592:Shewanella / Q000187:drug effects; D013696:Temperature; D062065:Wastewater / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D002701:Chloramphenicol; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Chloramphenicol; Degradation pathway; Microbial community; Microbial fuel cell