title: Simultaneous separation of neutral and cationic analytes by one dimensional open tubular capillary electrochromatography using zeolitic imidazolate framework-8 as stationary phase.
abstract: Developing simple methods for separation of analytes that belong to different classes is of great importance. Herein, we developed a simple one-dimensional (1-D) capillary electrochromatography method to demonstrate the simultaneous separation of target fractions that belong to two different classes (i.e., cationic and neutral analytes) without switching buffer solution by a zeolitic imidazolate framework ZIF-8 coated capillary column. Owing to the difference of charge-to-mass ratio of the cationic analytes, the interaction of the cationic analytes and coating material ZIF-8 and the hydrophobic interactions between the neutral analytes and the microporous framework of ZIF-8, six cationic analytes and four neutral analytes were simultaneously separated in a single run by 1-D capillary electrochromatography. The relative standard deviations (RSDs) of the analytes migration time for intra-day, inter-day and column-to-column were in the range of 0.11-0.87%, 0.54-2.04% and 2.00-6.89% and the RSDs of the analytes peak area for intra-day, inter-day and column-to-column were in the range of 0.70-4.45%, 1.33-6.20% and 2.27-11.88%. Additionally, the developed method was employed in the analysis of urine samples with satisfactory recoveries.
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
authors.forename: Congjie
authors.initials: C
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China. Electronic address: lvwenj@lzu.edu.cn.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Lv
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
authors.forename: Guoxiu
authors.initials: G
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Niu
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
authors.forename: Heying
authors.initials: H
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China; Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou 730000, China. Electronic address: chenxg@lzu.edu.cn.
authors.forename: Xingguo
authors.initials: X
authors.lastname: Chen
mesh_terms: D053801:Capillary Electrochromatography / Q000379:methods*; D002412:Cations; D017641:Zeolites / Q000737:chemistry*
chemical_list: D002412:Cations; D017641:Zeolites
keywords: Cationic and neutral analytes; One dimensional open tubular capillary electrochromatography; Simultaneous separation; Zeolitic imidazolate framework-8