title: In Vivo Effect of a 5-HT7Receptor Agonist on 5-HT Neurons and GABA Interneurons in the Dorsal Raphe Nuclei of Sham and PD Rats.
abstract: The 5-hydroxytryptamine (5-HT; serotonin) neurotransmission is severely affected by the degeneration of nigrostriatal dopaminergic neurons. Here, we report the effects of the systemic administration of the 5-HT7receptor agonist AS-19. In sham rats, the mean response of the 5-HT neurons in the dorsal raphe nucleus (DRN) to systemic AS-19 was excitatory and the mean response of the γ-aminobutyric acid (GABA) interneurons was inhibitory. In Parkinson disease (PD) rats, the same dose did not affect the 5-HT neurons and only high doses (640 μg/kg intravenous) were able to the increase GABA interneuron activity. These results indicate that DRN 5-HT neurons and GABA interneurons are regulated by the activation of 5-HT7receptors and that the degeneration of the nigrostriatal pathway leads to decreased responses of these neurons to AS-19, which in turn suggests that the 5-HT7receptors on 5-HT neurons and GABA interneurons in PD rats are dysfunctional and downregulated.
authors.affiliation: 1 Department of Pathophysiology, Institute of Basic Medical Science, Xi'an Medical University, Xi'an, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Wang
authors.affiliation: 1 Department of Pathophysiology, Institute of Basic Medical Science, Xi'an Medical University, Xi'an, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: 1 Department of Pathophysiology, Institute of Basic Medical Science, Xi'an Medical University, Xi'an, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.affiliation: 1 Department of Pathophysiology, Institute of Basic Medical Science, Xi'an Medical University, Xi'an, China.
authors.forename: Yufang
authors.initials: Y
authors.lastname: Guo
authors.affiliation: 1 Department of Pathophysiology, Institute of Basic Medical Science, Xi'an Medical University, Xi'an, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: 1 Department of Pathophysiology, Institute of Basic Medical Science, Xi'an Medical University, Xi'an, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Huo
authors.affiliation: 2 Department of Immunology, Institute of Basic Medical Science, Xi'an Medical University, Xi'an, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
authors.affiliation: 3 Department of Physiology, Institute of Basic Medical Science, Xi'an Medical University, Xi'an, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Cao
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D065847:Dorsal Raphe Nucleus / Q000187:drug effects*; D059330:GABAergic Neurons / Q000187:drug effects*; D007395:Interneurons / Q000187:drug effects*; D010300:Parkinson Disease / Q000378:metabolism*; D051381:Rats; D011985:Receptors, Serotonin / Q000378:metabolism*; D059326:Serotonergic Neurons / Q000187:drug effects*; D017366:Serotonin Receptor Agonists / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D011985:Receptors, Serotonin; D017366:Serotonin Receptor Agonists; C104425:serotonin 7 receptor
keywords: 5-HT neurons; 5-HT7 receptor; GABA interneurons; Parkinson disease; dorsal raphe nucleus; extracellular recordings