title: Dynamics of bacterial class Bacilli in the deepest valley lake of Kashmir-the Manasbal Lake.
abstract: In recognition of the importance of bacteria as ecological indicators of the aquatic systems a comprehensive and systematic analysis was carried out on Manasbal Lake, the deepest spring fed valley lake of Kashmir. The main objective envisaged was to analyze bacterial community composition (BCC) and for this purpose systematic and regular sampling of waters from ten different sampling stations, predetermined in the Lake according to differences in degree of human interference and also as zones of special ecological interests were selected. The isolated species were identified according to Bergey's Manual specification by examining their micro and macro morphological characteristics and biochemical characteristics on different culture media. Further confirmation was done by sequencing the 16s rRNA gene by using universal bacterial primers 27F and 1429R. From all the sampling stations the class Bacilli showed a maximum relative abundance with a contribution of 16 bacterial species. The whole process resulted in the identification of Bacillus aerius, Bacillus altitudinis, Bacillus anthracis, Bacillus cereus, Bacillus ginsengisoli, Bacillus pumilus, Bacillus safensis, Bacillus stratosphericus, Bacillus subtilis, Bacillus tequilensis, Bacillus thermocopriae, Bacillus thuringiensis, Brevibacillus agri strain, Lysinibacillus boronitolerans, Lysinibacillus pakistanensis and Lysinibacillus sphaericus.
authors.affiliation: Department of Environmental Science/Centre of Research for Development, University of Kashmir, Srinagar, J&K, 190 006, India.
authors.forename: Sana
authors.initials: S
authors.lastname: Shafi
authors.affiliation: Department of Environmental Science/Centre of Research for Development, University of Kashmir, Srinagar, J&K, 190 006, India.
authors.forename: Azra N
authors.initials: AN
authors.lastname: Kamili
authors.affiliation: Department of Botany, University of Kashmir, Srinagar, J&K, 190 006, India.
authors.forename: Manzoor A
authors.initials: MA
authors.lastname: Shah
authors.affiliation: Department of Environmental Science/Centre of Research for Development, University of Kashmir, Srinagar, J&K, 190 006, India. Electronic address: suhaibbandh@gmail.com.
authors.forename: Suhaib A
authors.initials: SA
authors.lastname: Bandh
authors.affiliation: Department of Environmental Science/Centre of Research for Development, University of Kashmir, Srinagar, J&K, 190 006, India; Department of Biotechnology, University of Kashmir, Srinagar, J&K, 190 006, India.
authors.forename: Rubiya
authors.initials: R
authors.lastname: Dar
mesh_terms: D001407:Bacillus / Q000145:classification* / Q000235:genetics; D015373:Bacterial Typing Techniques; D044822:Biodiversity; D058893:DNA Barcoding, Taxonomic; D005843:Geography; D060106:Lakes / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: 16s rRNA sequencing; BCC; Bacilli; Manasbal lake