title: Detection of urinary cell-free miR-210 as a potential tool of liquid biopsy for clear cell renal cell carcinoma.
abstract: Recent findings show that cell-free miRNAs are stable in biological fluid. Urine provides an alternative to blood serum or plasma as a potential source of tumor biomarkers. MiR-210 is proven to be overexpressed in patients with a clear cell renal cell carcinoma (ccRCC). The purpose of this pilot study was to examine the urinary cell-free miR-210 as a potential tool of liquid biopsy for ccRCC.
  
  
  
  Overall, 75 patients with a ccRCC and 45 control subjects without a cancer were included in this study. Urine samples were centrifuged twice and the cell-free urine supernanants were stored in -80°C until use. A total of 350µl cell-free urine was used for the extraction of total RNA. The expression levels of these miRNAs were performed by using quantitative RT-PCR.
  
  
  
  The level of urinary cell-free miR-210 was significantly higher in patients with ccRCC than in control subjects (P<0.001). The urinary cell-free miR-210 yielded the areas under the receiver operating characteristics curve of 0.76 in discriminating the patients with ccRCC from the control subjects with a sensitivity of 57.8% and a specificity of 80.0%. Moreover, the expression level of urinary cell-free miRNA-210 was significantly decreased in the patients a week after surgery (P<0.001).
  
  
  
  Urinary cell-free miR-210 may be used as a potential tool of liquid biopsy for ccRCC diagnosis. Further studies are needed to confirm our results.
authors.affiliation: Department of Urology, North Hospital, CHU of Saint-Etienne, University of Jean-Monnet, Saint-Etienne, France; Inserm U1059, Saint-Etienne, France. Electronic address: grli2001@yahoo.fr.
authors.forename: Guorong
authors.initials: G
authors.lastname: Li
authors.affiliation: Zhejiang Cancer Research Institute, Zhejiang Province Cancer Hospital, Hangzhou, China. Electronic address: anzmxx@gmail.com.
authors.forename: An
authors.initials: A
authors.lastname: Zhao
authors.affiliation: Laboratory of Pathology, North Hospital, CHU of Saint-Etienne, University of Jean-Monnet, Saint-Etienne, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Péoch
authors.affiliation: Laboratory of Cytology, North Hospital, CHU of Saint-Etienne, University of Jean-Monnet, Saint-Etienne, France.
authors.forename: Michèle
authors.initials: M
authors.lastname: Cottier
authors.affiliation: Department of Urology, North Hospital, CHU of Saint-Etienne, University of Jean-Monnet, Saint-Etienne, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Mottet
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000652:urine; D002292:Carcinoma, Renal Cell / Q000601:surgery / Q000652:urine*; D016022:Case-Control Studies; D000074166:Circulating MicroRNA / Q000652:urine*; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000601:surgery / Q000652:urine*; D000073890:Liquid Biopsy; D008297:Male; D035683:MicroRNAs / Q000652:urine*; D008875:Middle Aged; D010865:Pilot Projects; D011184:Postoperative Period; D057234:Preoperative Period; D012372:ROC Curve; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D000074166:Circulating MicroRNA; C543800:MIRN210 microRNA, human; D035683:MicroRNAs
keywords: Cell-free urine; Clear cell renal cell carcinoma; Liquid biopsy; miR-210