title: Circular BANP, an upregulated circular RNA that modulates cell proliferation in colorectal cancer.
abstract: Circular RNAs (circRNAs) are recently identified as widespread and diverse endogenous noncoding RNAs that may harbor vital functions in human and animals. However, the role of circRNAs in the process of tumorigenesis and development of colorectal cancer (CRC) remains vague. Here we characterized the circRNA expression profile from three paired CRC cancerous and adjacent normal tissues by human circRNA array, and identified 136 significantly overexpressed circRNAs and 243 downregulated circRNAs in CRC cancerous tissues (>2-fold changes). We further validated one circRNA generated from Exon 5-11 of BANP gene, termed circ-BANP. In addition, RT-PCR result showed that circ-BANP was overexpressed in 35 CRC cancerous tissues. Knockdown of circ-BANP with siRNA significantly attenuate the proliferation of CRC cells. In summary, our findings demonstrated that dysregulated circ-BANP appears to have an important role in CRC cells and could serve as a prognostic and therapeutic marker for CRC.
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing 211166, China; Department of Clinical Laboratory, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical University Affiliated Cancer Hospital, Nanjing 210009, China.
authors.forename: Mingchen
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Department of Gastroenterology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Xu
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing 211166, China. Electronic address: ychen@njmu.edu.cn.
authors.forename: Yun
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Clinical Laboratory, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical University Affiliated Cancer Hospital, Nanjing 210009, China. Electronic address: yanfeng2007@sohu.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Yan
mesh_terms: D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000235:genetics*; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000235:genetics*; D012313:RNA / Q000235:genetics* / Q000378:metabolism; D000079962:RNA, Circular; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D015854:Up-Regulation / Q000235:genetics*
chemical_list: C417914:BANP protein, human; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D000079962:RNA, Circular; D012333:RNA, Messenger; D012313:RNA
keywords: BANP; Circular RNA; Colorectal cancer; Proliferation; siRNA