title: Determination of Four Nadolol Stereoisomers in Capsules by High-Performance Liquid Chromatography and Circular Dichroism.
abstract: Nadolol is a blocking agent with activity in β-adrenergic receptors. The objective of this research was to develop and validate an HPLC and circular dichroism method for the quantification of four nadolol stereoisomers in capsules. The HPLC method was validated using a Chiralcel OD column (250 × 4,6 mm, 10 μm). The mobile phase consisted of hexane-ethanol-diethylamine-acetic acid (86 + 14 + 0.3 + 0.3, v/v/v/v), with a flow rate of 0.7 mL/min and temperature of 25 ± 1°C and UV detection carried out at 220 nm. Solutions were prepared in ethanol containing 200 μg/mL nadolol. The method proved to be precise, selective, accurate, and robust and was successfully applied for the determination of the two homologs of four nadolol stereoisomers in capsules.
authors.affiliation: University of São Paulo, Faculty of Pharmaceutical Sciences, Department of Pharmacy, Avenida Professor Lineu Prestes 580, CEP 05508-000 São Paulo, SP, Brazil.
authors.forename: Grazielle
authors.initials: G
authors.lastname: Prado Alexandre
authors.affiliation: University of São Paulo, Faculty of Pharmaceutical Sciences, Department of Pharmacy, Avenida Professor Lineu Prestes 580, CEP 05508-000 São Paulo, SP, Brazil.
authors.forename: Márcio Nardelli
authors.initials: MN
authors.lastname: Wandermuren
authors.affiliation: University of São Paulo, Faculty of Pharmaceutical Sciences, Department of Pharmacy, Avenida Professor Lineu Prestes 580, CEP 05508-000 São Paulo, SP, Brazil.
authors.forename: Adriano
authors.initials: A
authors.lastname: Peron
authors.affiliation: University of São Paulo, Faculty of Pharmaceutical Sciences, Department of Pharmacy, Avenida Professor Lineu Prestes 580, CEP 05508-000 São Paulo, SP, Brazil.
authors.forename: Vanessa Franco
authors.initials: VF
authors.lastname: Tavares
authors.affiliation: University of São Paulo, Faculty of Pharmaceutical Sciences, Department of Pharmacy, Avenida Professor Lineu Prestes 580, CEP 05508-000 São Paulo, SP, Brazil.
authors.forename: Denis Alexandre
authors.initials: DA
authors.lastname: Ramalho Padeiro
authors.affiliation: University of São Paulo, Faculty of Pharmaceutical Sciences, Department of Pharmacy, Avenida Professor Lineu Prestes 580, CEP 05508-000 São Paulo, SP, Brazil.
authors.forename: Kelly Alessandra
authors.initials: KA
authors.lastname: Sakiara
authors.affiliation: University of São Paulo, Faculty of Pharmaceutical Sciences, Department of Pharmacy, Avenida Professor Lineu Prestes 580, CEP 05508-000 São Paulo, SP, Brazil.
authors.forename: María Segunda
authors.initials: MS
authors.lastname: Aurora-Prado
authors.affiliation: University of São Paulo, Faculty of Pharmaceutical Sciences, Department of Pharmacy, Avenida Professor Lineu Prestes 580, CEP 05508-000 São Paulo, SP, Brazil.
authors.forename: Anil Kumar
authors.initials: AK
authors.lastname: Singh
authors.affiliation: University of São Paulo, Faculty of Pharmaceutical Sciences, Department of Pharmacy, Avenida Professor Lineu Prestes 580, CEP 05508-000 São Paulo, SP, Brazil.
authors.forename: Erika Rosa
authors.initials: ER
authors.lastname: Kedor-Hackmann
authors.affiliation: University of São Paulo, Faculty of Pharmaceutical Sciences, Department of Pharmacy, Avenida Professor Lineu Prestes 580, CEP 05508-000 São Paulo, SP, Brazil.
authors.forename: Maria Inês Rocha
authors.initials: MIR
authors.lastname: Miritello Santoro
mesh_terms: D002214:Capsules; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D009248:Nadolol / Q000032:analysis*; D013237:Stereoisomerism
chemical_list: D002214:Capsules; D009248:Nadolol