title: Fast methods of fungal and bacterial identification. MALDI-TOF mass spectrometry, chromogenic media.
abstract: MALDI-TOF mass spectrometry is now a routine resource in Clinical Microbiology, because of its speed and reliability in the identification of microorganisms. Its performance in the identification of bacteria and yeasts is perfectly contrasted. The identification of mycobacteria and moulds is more complex, due to the heterogeneity of spectra within each species. The methodology is somewhat more complex, and expanding the size of species libraries, and the number of spectra of each species, will be crucial to achieve greater efficiency. Direct identification from blood cultures has been implemented, since its contribution to the management of severe patients is evident, but its application to other samples is more complex. Chromogenic media have also contributed to the rapid diagnosis in both bacteria and yeast, since they accelerate the diagnosis, facilitate the detection of mixed cultures and allow rapid diagnosis of resistant species.
authors.affiliation: Instituto de Investigación Biomédica de Salamanca (IBSAL), Complejo Asistencial Universitario de Salamanca, Universidad de Salamanca, CSIC, Salamanca, España.
authors.forename: María
authors.initials: M
authors.lastname: Siller-Ruiz
authors.affiliation: Instituto de Investigación Biomédica de Salamanca (IBSAL), Complejo Asistencial Universitario de Salamanca, Universidad de Salamanca, CSIC, Salamanca, España.
authors.forename: Sara
authors.initials: S
authors.lastname: Hernández-Egido
authors.affiliation: Instituto de Investigación Biomédica de Salamanca (IBSAL), Complejo Asistencial Universitario de Salamanca, Universidad de Salamanca, CSIC, Salamanca, España.
authors.forename: Fernando
authors.initials: F
authors.lastname: Sánchez-Juanes
authors.affiliation: Instituto de Investigación Biomédica de Salamanca (IBSAL), Complejo Asistencial Universitario de Salamanca, Universidad de Salamanca, CSIC, Salamanca, España; Servicio de Bioquímica Clínica, Complejo Asistencial Universitario de Salamanca, Salamanca, España; Departamento de Bioquímica y Biología Molecular, Universidad de Salamanca, Salamanca, España.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: González-Buitrago
authors.affiliation: Instituto de Investigación Biomédica de Salamanca (IBSAL), Complejo Asistencial Universitario de Salamanca, Universidad de Salamanca, CSIC, Salamanca, España; Servicio de Microbiología, Complejo Asistencial Universitario de Salamanca, Salamanca, España; Departamento de Ciencias Biomédicas y del Diagnóstico, Universidad de Salamanca, Salamanca, España. Electronic address: jlmubel@usal.es.
authors.forename: Juan Luis
authors.initials: JL
authors.lastname: Muñoz-Bellido
mesh_terms: D001419:Bacteria / Q000145:classification / Q000302:isolation & purification; D001424:Bacterial Infections / Q000382:microbiology; D015373:Bacterial Typing Techniques / Q000379:methods*; D001826:Body Fluids / Q000382:microbiology; D002863:Chromogenic Compounds; D003470:Culture Media; D005658:Fungi / Q000145:classification / Q000302:isolation & purification; D006801:Humans; D016533:Mycological Typing Techniques / Q000379:methods*; D009181:Mycoses / Q000382:microbiology; D013048:Specimen Handling; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013194:Staining and Labeling; D013997:Time Factors
chemical_list: D002863:Chromogenic Compounds; D003470:Culture Media
keywords: Chromogenic media; Espectrometría de masas MALDI-TOF; Identificación; Identification; MALDI-TOF mass spectrometry; Medios cromogénicos