title: Deregulated Cardiac Specific MicroRNAs in Postnatal Heart Growth.
abstract: The heart is recognized as an organ that is terminally differentiated by adulthood. However, during the process of human development, the heart is the first organ with function in the embryo and grows rapidly during the postnatal period. MicroRNAs (miRNAs, miRs), as regulators of gene expression, play important roles during the development of multiple systems. However, the role of miRNAs in postnatal heart growth is still unclear. In this study, by using qRT-PCR, we compared the expression of seven cardiac- or muscle-specific miRNAs that may be related to heart development in heart tissue from mice at postnatal days 0, 3, 8, and 14. Four miRNAs-miR-1a-3p, miR-133b-3p, miR-208b-3p, and miR-206-3p-were significantly decreased while miR-208a-3p was upregulated during the postnatal heart growth period. Based on these results, GeneSpring GX was used to predict potential downstream targets by performing a 3-way comparison of predictions from the miRWalk, PITA, and microRNAorg databases. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were used to identify potential functional annotations and signaling pathways related to postnatal heart growth. This study describes expression changes of cardiac- and muscle-specific miRNAs during postnatal heart growth and may provide new therapeutic targets for cardiovascular diseases.
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China.
authors.forename: Pujiao
authors.initials: P
authors.lastname: Yu
authors.affiliation: Department of Cardiology, Yangpu Hospital, Tongji University School of Medicine, Shanghai 200090, China.
authors.forename: Hongbao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China.
authors.forename: Jinzhe
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Cardiology, Yangpu Hospital, Tongji University School of Medicine, Shanghai 200090, China.
authors.forename: Jianhua
authors.identifier: 0000-0002-0980-705X
authors.initials: J
authors.lastname: Yao
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China.
authors.forename: Lin
authors.identifier: 0000-0002-6107-6012
authors.initials: L
authors.lastname: Che
mesh_terms: D000818:Animals; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006321:Heart / Q000254:growth & development*; D006801:Humans; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis*
chemical_list: D035683:MicroRNAs