title: Dengue infection in pregnancy and its impact on the placenta.
abstract: A histopathological and immunohistochemical study was conducted in placental tissues and retained products of conception from 24 patients with confirmed dengue infection during pregnancy. The immunohistochemical assay was positive for dengue virus in 19 placental and three ovular remnants analyzed. The light microscopic findings were signs of hypoxia, choriodeciduitis, deciduitis and intervillositis and the viral antigens were found in cytoplasmic of the trophoblast, villous stroma and decidua. Our results suggest that immunohistochemistry could be used as a laboratory confirmation method for dengue in pregnant women, especially in endemic areas when embedded material is the only material available.
authors.affiliation: Departamento Materno-infantil, Universidade Federal Fluminense, 303, Marquês do Paraná Street, 3° floor, Centro, Niterói, Rio de Janeiro 24033-900, Brazil. Electronic address: chrisribeiro@vm.uff.br.
authors.forename: Christiane Fernandes
authors.initials: CF
authors.lastname: Ribeiro
authors.affiliation: Department of Pathology, Universidade Federal Fluminense, 303, Marquês do Paraná Street, Centro, Niterói, Rio de Janeiro, 24033-900, Brazil. Electronic address: vasilami@oul.com.br.
authors.forename: Vânia Glória Silami
authors.initials: VGS
authors.lastname: Lopes
authors.affiliation: 'Evandro Chagas' National Institute of Infectology [Instituto Nacional de Infectologia Evandro Chagas]/Fiocruz, Rio de Janeiro, Brazil. Electronic address: patricia.brasil@ini.fiocruz.br.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Brasil
authors.affiliation: Department of Pathology, Universidade Federal Fluminense, 303, Marquês do Paraná Street, Centro, Niterói, Rio de Janeiro, 24033-900, Brazil. Electronic address: andreapires@fontemd.com.br.
authors.forename: Andrea Rodrigues Cordovil
authors.initials: ARC
authors.lastname: Pires
authors.affiliation: Intensivist, Head of maternal-fetal intensive care unit of Perinatal de Laranjeiras and Barra da Tijuca, Brazil. Electronic address: roger.rohloff@gmail.com.
authors.forename: Roger
authors.initials: R
authors.lastname: Rohloff
authors.affiliation: Flavirus Laboratory, Oswaldo Cruz Institute/Fiocruz, Brazil. Electronic address: rita@ioc.fiocruz.br.
authors.forename: Rita Maria Ribeiro
authors.initials: RMR
authors.lastname: Nogueira
mesh_terms: D000293:Adolescent; D000328:Adult; D003656:Decidua / Q000276:immunology; D003715:Dengue / Q000276:immunology / Q000473:pathology*; D003716:Dengue Virus / Q000276:immunology; D005260:Female; D005865:Gestational Age; D006801:Humans; D007150:Immunohistochemistry; D007231:Infant, Newborn; D010920:Placenta / Q000276:immunology / Q000473:pathology*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000276:immunology / Q000473:pathology / Q000821:virology*; D011256:Pregnancy Outcome; D014327:Trophoblasts / Q000276:immunology / Q000473:pathology; D055815:Young Adult
keywords: dengue; placenta; pregnancy; vertical transmission