title: The use of rockfish Sebastiscus marmoratus as a sentinel species to assess petroleum hydrocarbons pollution: A case study in Quanzhou Bay, China.
abstract: To monitor the biological effects of marine pollution, choosing a native fish species and establishing suitable biomarkers are required. In this study, the full-length cDNA of cyp1a1 was cloned from Sebastiscus marmoratus (SM-CYP1A1). Then the dose-response and time-course induction of hepatic CYP1A1 mRNA by the crude oil water-soluble fraction (WSF) were determined. Subsequently, SM-CYP1A1 mRNA was applied to investigate the biological effect of petroleum hydrocarbon pollution in Quanzhou Bay, China. The transcription levels of hepatic CYP1A1 were significantly elevated in fish caged in the polluted sites for 2weeks compared with those of the reference site, which were correlated with the concentrations of petroleum hydrocarbon and polycyclic aromatic hydrocarbon (PAH) in the surface seawaters. The results suggest that S. marmoratus is a potential sentinel organism to monitor marine pollutants and the hepatic CYP1A1 mRNA can serve as a sensitive biomarker to organic xenobiotics in aquatic environments.
authors.affiliation: Third Institute of Oceanography, State Oceanic Administration, Xiamen, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Bo
authors.affiliation: Third Institute of Oceanography, State Oceanic Administration, Xiamen, China.
authors.forename: Ronghui
authors.initials: R
authors.lastname: Zheng
authors.affiliation: Third Institute of Oceanography, State Oceanic Administration, Xiamen, China.
authors.forename: Weiming
authors.initials: W
authors.lastname: Kuang
authors.affiliation: Third Institute of Oceanography, State Oceanic Administration, Xiamen, China.
authors.forename: Fukun
authors.initials: F
authors.lastname: Hong
authors.affiliation: Third Institute of Oceanography, State Oceanic Administration, Xiamen, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Xie
authors.affiliation: Third Institute of Oceanography, State Oceanic Administration, Xiamen, China. Electronic address: zhangyusheng@tio.org.cn.
authors.forename: Yusheng
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D061006:Bays; D002681:China; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004781:Environmental Exposure; D004784:Environmental Monitoring; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D005399:Fishes / Q000235:genetics* / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000662:veterinary; D010578:Petroleum / Q000009:adverse effects*; D059392:Petroleum Pollution; D010802:Phylogeny; D011084:Polycyclic Aromatic Hydrocarbons / Q000009:adverse effects*; D000072739:Sentinel Species / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment / Q000662:veterinary; D014874:Water Pollutants, Chemical / Q000009:adverse effects*
chemical_list: D029941:Fish Proteins; D010578:Petroleum; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D019363:Cytochrome P-450 CYP1A1
keywords: Cytochrome 4501A1; Field monitoring; Petroleum hydrocarbon; Polycyclic aromatic hydrocarbons (PAHs); Sebastiscus marmoratus