title: Development of SSR markers by 454 sequencing in the endemic speciesGentianella praecoxsubsp.bohemica(Gentianaceae).
abstract: OBJECTIVE
  Polymorphic microsatellite loci were developed and used to genotype individuals ofGentianella praecoxsubsp.bohemica(Gentianaceae), a highly protected taxon in Europe, to study the genetic structure of the remaining populations.
  
  
  RESULTS
  Thirty-eight primer pairs were successfully amplified; of these, 12 polymorphic microsatellite loci were developed using a 454 sequencing approach and used to genotype 180 individuals ofG. praecoxsubsp.bohemicafrom six populations. Allelic richness ranged between one and nine alleles per locus. We detected a high frequency of polyploid individuals (77.8%). The highest average percentage of heterozygous genotypes was identified for samples from the Hroby population (75.5%). All loci can also be amplified in the congeneric speciesG. praecoxsubsp.praecox,G. amarellasubsp.amarella, andG. obtusifoliasubsp.sturmiana.
  
  
  CONCLUSIONS
  These markers will provide knowledge on patterns of gene flow and population genetic structure, which is necessary for current protection actions and for effective conservation of this species in the future.
authors.affiliation: Institute of Botany of the Czech Academy of Sciences, Zámek 1, 252 43 Průhonice, Czech Republic; Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01 Prague, Czech Republic.
authors.forename: Mária
authors.initials: M
authors.lastname: Šurinová
authors.affiliation: Muzeum Cheb, Krále Jiřího z Poděbrad 493/4, 350 11 Cheb, Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Brabec
authors.affiliation: Institute of Botany of the Czech Academy of Sciences, Zámek 1, 252 43 Průhonice, Czech Republic; Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01 Prague, Czech Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Münzbergová
keywords: Gentianaceae; Gentianella praecox subsp. bohemica; genotyping; microsatellites; polyploidy