title: Core‑shell lipid polymer nanoparticles for combined chemo and gene therapy of childhood head and neck cancers.
abstract: Pediatric head and neck cancers account for overall 12% of all pediatric cancers. Despite recent advances in therapeutic modalities, children with tumor metastasis have poor prognosis. Therefore, there is an unmet need for new and effective treatment modalities for pediatric head and neck cancers. The present study describes a simple and efficient method for fabrication of cationic lipid‑polymer hybrid nanoparticles (CLPNs) for co‑delivery of cisplatin (CDDP) and DNA (CDDP/DNA CLPNs) for the therapy of childhood head and neck cancers. CDDP/DNA CLPNs were prepared by the modified double emulsion solvent evaporation method with self‑assembly. CDDP‑loaded CLPNs (CDDP CLPNs), CDDP-loaded polymeric nanoparticles (PNPs) (CDDP PNPs), and DNA‑loaded Lipofectamine® 2000 (DNA LIPO) were also prepared for comparison. The results illustrated that the concentration of the cationic lipid has influence on the characteristics of CLPNs. In vitro anticancer effect, in vitro transfection efficiency, in vivo antitumor and gene delivery efficacy of CDDP/DNA CLPNs have advantages over other formulations tested. In conclusion, outstanding delivery ability of CLPNs for both CDDP and DNA could combine the therapeutic efficiency of both drug and gene for the treatment of pediatric rhabdomyosarcoma (RMS).
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Kunming Children's Hospital, Kunming, Yunnan 650032, P.R. China.
authors.forename: Tiesong
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Kunming Children's Hospital, Kunming, Yunnan 650032, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Anesthesiology, Kunming Children's Hospital, Kunming, Yunnan 650032, P.R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Kunming Children's Hospital, Kunming, Yunnan 650032, P.R. China.
authors.forename: Ken
authors.initials: K
authors.lastname: Lin
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Kunming Children's Hospital, Kunming, Yunnan 650032, P.R. China.
authors.forename: Fan
authors.initials: F
authors.lastname: Lou
authors.affiliation: Department of Clinical Laboratory, Kunming Children's Hospital, Kunming, Yunnan 650032, P.R. China.
authors.forename: Hongchao
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Kunming Children's Hospital, Kunming, Yunnan 650032, P.R. China.
authors.forename: Yingqin
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, P.R. China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Otolaryngology, The Third People's Hospital of Yunnan Province, Kunming, Yunnan 650032, P.R. China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Ming
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, P.R. China.
authors.forename: Biao
authors.initials: B
authors.lastname: Ruan
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002470:Cell Survival; D002648:Child; D002945:Cisplatin / Q000494:pharmacology*; D003131:Combined Modality Therapy; D004337:Drug Carriers; D016503:Drug Delivery Systems; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D015316:Genetic Therapy; D006258:Head and Neck Neoplasms / Q000235:genetics / Q000628:therapy*; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D008055:Lipids; C086724:Lipofectamine; D011108:Polymers; D002945:Cisplatin