title: Dalbergioidin Ameliorates Doxorubicin-Induced Renal Fibrosis by Suppressing the TGF-βSignal Pathway.
abstract: We investigated the effect of Dalbergioidin (DAL), a well-known natural product extracted fromUraria crinita, on doxorubicin- (DXR-) induced renal fibrosis in mice. The mice were pretreated for 7 days with DAL followed by a single injection of DXR (10 mg/kg) via the tail vein. Renal function was analyzed 5 weeks after DXR treatment. DXR caused nephrotoxicity. The symptoms of nephrotic syndrome were greatly improved after DAL treatment. The indices of renal fibrosis, the phosphorylation of Smad3, and the expression of alpha-smooth muscle actin (α-SMA), fibronectin, collagen III (Col III), E-cadherin, TGF-β, and Smad7 in response to DXR were all similarly modified by DAL. The present findings suggest that DAL improved the markers for kidney damage investigated in this model of DXR-induced experimental nephrotoxicity.
authors.affiliation: National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China; Department of Pediatrics, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China.
authors.forename: Xianguo
authors.initials: X
authors.lastname: Ren
authors.affiliation: Department of Geriatrics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Bo
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing, China.
authors.forename: Junting
authors.initials: J
authors.lastname: Fan
authors.affiliation: Department of Nephrology, Zhejiang Provincial People's Hospital, Hangzhou, China.
authors.forename: Maosheng
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Nephrology, Anhui Provincial Children's Hospital, Hefei, China; Nanjing University of Traditional Chinese Medicine, Nanjing, China.
authors.forename: Daliang
authors.initials: D
authors.lastname: Xu
authors.affiliation: Department of Nephrology, Anhui Provincial Children's Hospital, Hefei, China; Nanjing University of Traditional Chinese Medicine, Nanjing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Department of Urology, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China.
authors.forename: Haowei
authors.initials: H
authors.lastname: He
authors.affiliation: Nanjing University of Traditional Chinese Medicine, Nanjing, China.
authors.forename: Xianzhi
authors.initials: X
authors.lastname: Ren
authors.affiliation: Department of Nephrology, Anhui Provincial Children's Hospital, Hefei, China; Nanjing University of Traditional Chinese Medicine, Nanjing, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Qu
authors.affiliation: Department of Nephrology, Anhui Provincial Children's Hospital, Hefei, China; Nanjing University of Traditional Chinese Medicine, Nanjing, China.
authors.forename: Yulian
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Geriatrics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Weihong
authors.identifier: 0000-0001-7809-6020
authors.initials: W
authors.lastname: Zhao
authors.affiliation: National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China; Nanjing University of Traditional Chinese Medicine, Nanjing, China.
authors.forename: Changliang
authors.identifier: 0000-0001-6972-8460
authors.initials: C
authors.lastname: Xu
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000009:adverse effects; D015820:Cadherins / Q000378:metabolism; D002867:Chromones / Q000627:therapeutic use*; D024061:Collagen Type III / Q000378:metabolism; D004317:Doxorubicin / Q000009:adverse effects*; D005353:Fibronectins / Q000378:metabolism; D005355:Fibrosis / Q000139:chemically induced / Q000188:drug therapy*; D005978:Glutathione / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D007674:Kidney Diseases / Q000378:metabolism; D051379:Mice; D009130:Muscle, Smooth / Q000378:metabolism; D018696:Neuroprotective Agents / Q000627:therapeutic use; D010084:Oxidation-Reduction; D010766:Phosphorylation; D010936:Plant Extracts / Q000627:therapeutic use; D011897:Random Allocation; D015398:Signal Transduction; D051900:Smad3 Protein / Q000378:metabolism; D051906:Smad7 Protein / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors*
chemical_list: D000199:Actins; D000903:Antibiotics, Antineoplastic; D015820:Cadherins; D002867:Chromones; D024061:Collagen Type III; D005353:Fibronectins; D018696:Neuroprotective Agents; D010936:Plant Extracts; D051900:Smad3 Protein; C497791:Smad3 protein, mouse; D051906:Smad7 Protein; C497807:Smad7 protein, mouse; D016212:Transforming Growth Factor beta; C531366:dalbergioidin; D004317:Doxorubicin; D005978:Glutathione