title: Genome wide occurrence and insertion preferences of INGI/RIME and SLACS CRE transposable elements inTrypanosoma brucei.
abstract: Retrotransposons play significant role in genome remodelling ofT. bruceiand about 5% of its genome consists of retrotransposons including INGI/RIME elements. INGI is one of the dispersed repetitive elements inT. bruceigenome which is found distributed throughout all the chromosomes. SLACS (Spliced Leader Associated Conserved Sequence) however, is more conserved in its nature and lacks the typical poly-distributional pattern of LINE like transposons. We have found total 589 copies of these TEs with only 17.06 % (104 copies out of 589) copies with both ends intact thus showing a majority of truncated copies. Complete SLACS CRE were found only on chromosome 9, whereas, complete INGI/RIME were more ubiquitously distributed. The 50 - 70 bp upstream flanking sequence of these elements shows suitable nucleotide biophysical properties to favor transposition.
authors.affiliation: Department of Plant Sciences, M.J.P. Rohilkhand University, Bareilly, UP, India; Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Bareilly, UP, India.
authors.forename: Mohd Faheem
authors.initials: MF
authors.lastname: Khan
authors.affiliation: Division of Animal Genetics, Indian Veterinary Research Institute , Izatnagar, Bareilly, UP, India.
authors.forename: Kush
authors.initials: K
authors.lastname: Shrivastava
authors.affiliation: Dairy Cattle Breeding Division, National Dairy Research Institute , Karnal, Haryana, India.
authors.forename: Rebeka
authors.initials: R
authors.lastname: Sinha
authors.affiliation: Department of Plant Sciences, M.J.P. Rohilkhand University , Bareilly, UP, India.
authors.forename: Virendra
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Department of Plant Sciences, M.J.P. Rohilkhand University , Bareilly, UP, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Jaitly
keywords: INGI/RIME; SLACS CRE; T. brucei; TEs; distribution; insertion preferences