title: Ubiquitin specific protease 21 upregulation in breast cancer promotes cell tumorigenic capability and is associated with the NOD-like receptor signaling pathway.
abstract: Ubiquitination and deubiquitination have emerged as critical regulators in cancer. In the present study, the expression pattern of 50 ubiquitin-specific proteases (USPs) was summarized in breast cancer using a bioinformatics approach, and USP21 was identified as the most altered gene in breast cancer. In particular, expression of USP21 in triple negative breast cancer (TNBC) cell lines was greater compared with other subtypes of breast cancer. Knockdown of USP21 in TNBC cells inhibited cell proliferation, migration and invasion. Microarray profiling of the USP21 knockdown cells revealed significant downregulation of multiple genes associated with the NOD-like receptor signaling pathway. The results of the present study suggest that USP21 has a significant role in TNBC progression, and therefore may represent a novel therapeutic target.
authors.affiliation: Department of Oncology, Chinese PLA General Hospital, Beijing 100853, P.R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Peng
authors.affiliation: Department of Oncology, Chinese PLA General Hospital, Beijing 100853, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Hu
authors.affiliation: School of Dentistry, University of California, Los Angeles, CA 90095, USA.
authors.forename: Demeng
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Oncology, Chinese PLA General Hospital, Beijing 100853, P.R. China.
authors.forename: Ruixia
authors.initials: R
authors.lastname: Linghu
authors.affiliation: Department of Oncology, Chinese PLA General Hospital, Beijing 100853, P.R. China.
authors.forename: Yingzhe
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Oncology, Chinese PLA General Hospital, Beijing 100853, P.R. China.
authors.forename: Xiaoxue
authors.initials: X
authors.lastname: Kou
authors.affiliation: Department of Oncology, Chinese PLA General Hospital, Beijing 100853, P.R. China.
authors.forename: Junlan
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Oncology, Chinese PLA General Hospital, Beijing 100853, P.R. China.
authors.forename: Shunchang
authors.initials: S
authors.lastname: Jiao
keywords: nucleotide oligomerization domain-like receptor signaling pathway; relA; triple negative breast cancer; ubiquitin specific protease 21