title: Salt stress-induced changes in antioxidative defense system and proteome profiles of salt-tolerant and sensitive Frankia strains.
abstract: An appreciation of comparative microbial survival is most easily done while evaluating their adaptive strategies during stress. In the present experiment, antioxidative and whole cell proteome variations based on spectrophotometric analysis and SDS-PAGE and 2-dimensional gel electrophoresis have been analysed among salt-tolerant and salt-sensitive Frankia strains. This is the first report of proteomic basis underlying salt tolerance in these newly isolated Frankia strains from Hippophae salicifolia D. Don. Salt-tolerant strain HsIi10 shows higher increment in the contents of superoxide dismutase, catalase and ascorbate peroxidase as compared to salt-sensitive strain HsIi8. Differential 2-DGE profile has revealed differential profiles for salt-tolerant and salt-sensitive strains. Proteomic confirmation of salt tolerance in the strains with inbuilt efficiency of thriving in nitrogen-deficient locales is a definite advantage for these microbes. This would be equally beneficial for improvement of soil nitrogen status. Efficient protein regulation in HsIi10 suggests further exploration for its potential use as biofertilizer in saline soils.
authors.affiliation: a Laboratory of Microbial Genetics, Department of Botany , Banaras Hindu University , Varanasi , India.|b Life Science Programme , Central University of South Bihar , Patna , India.
authors.forename: Amrita
authors.initials: A
authors.lastname: Srivastava
authors.affiliation: a Laboratory of Microbial Genetics, Department of Botany , Banaras Hindu University , Varanasi , India.
authors.forename: Anumeha
authors.initials: A
authors.lastname: Singh
authors.affiliation: a Laboratory of Microbial Genetics, Department of Botany , Banaras Hindu University , Varanasi , India.|c Department of Botany , Guru Ghasidas Vishwavidyalaya , Bilaspur , Chhattisgarh , India.
authors.forename: Satya S
authors.initials: SS
authors.lastname: Singh
authors.affiliation: a Laboratory of Microbial Genetics, Department of Botany , Banaras Hindu University , Varanasi , India.
authors.forename: Arun K
authors.initials: AK
authors.lastname: Mishra
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D040161:Frankia / Q000737:chemistry / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D018384:Oxidative Stress; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D055049:Salt Tolerance; D012965:Sodium Chloride / Q000378:metabolism; D013312:Stress, Physiological
chemical_list: D000975:Antioxidants; D020543:Proteome; D012965:Sodium Chloride; D002374:Catalase; D009584:Nitrogen
keywords: 2-dimensional gel electrophoresis; Biofertilizer; Frankia; salt tolerance