title: Intravascular Ultrasound Characterization of a Tissue-Engineered Vascular Graft in an Ovine Model.
abstract: Patients who undergo implantation of a tissue-engineered vascular graft (TEVG) for congenital cardiac anomalies are monitored with echocardiography, followed by magnetic resonance imaging or angiography when indicated. While these methods provide data regarding the lumen, minimal information regarding neotissue formation is obtained. Intravascular ultrasound (IVUS) has previously been used in a variety of conditions to evaluate the vessel wall. The purpose of this study was to evaluate the utility of IVUS for evaluation of TEVGs in our ovine model. Eight sheep underwent implantation of TEVGs either unseeded or seeded with bone marrow-derived mononuclear cells. Angiography, IVUS, and histology were directly compared. Endothelium, tunica media, and graft were identifiable on IVUS and histology at multiple time points. There was strong agreement between IVUS and angiography for evaluation of luminal diameter. IVUS offers a valuable tool to evaluate the changes within TEVGs, and clinical translation of this application is warranted.
authors.affiliation: Tissue Engineering Program, Research Institute at Nationwide Children's Hospital, 700 Children's Drive, WB 4154, Columbus, OH, 43205, USA.|Department of Pediatric Surgery, Nationwide Children's Hospital, Columbus, OH, USA.
authors.forename: Victoria K
authors.initials: VK
authors.lastname: Pepper
authors.affiliation: Tissue Engineering Program, Research Institute at Nationwide Children's Hospital, 700 Children's Drive, WB 4154, Columbus, OH, 43205, USA.|College of Veterinary Medicine, The Ohio State University, Columbus, OH, USA.
authors.forename: Elizabeth S
authors.initials: ES
authors.lastname: Clark
authors.affiliation: Tissue Engineering Program, Research Institute at Nationwide Children's Hospital, 700 Children's Drive, WB 4154, Columbus, OH, 43205, USA.
authors.forename: Cameron A
authors.initials: CA
authors.lastname: Best
authors.affiliation: Tissue Engineering Program, Research Institute at Nationwide Children's Hospital, 700 Children's Drive, WB 4154, Columbus, OH, 43205, USA.|Department of Surgery, The Ohio State University Wexner Medical Center, Columbus, OH, USA.
authors.forename: Ekene A
authors.initials: EA
authors.lastname: Onwuka
authors.affiliation: Division of Cardiothoracic Transplant & Mechanical Circulatory Support, Texas Heart Institute at Baylor St. Luke's Medical Center, Houston, TX, USA.
authors.forename: Tadahisa
authors.initials: T
authors.lastname: Sugiura
authors.affiliation: Tissue Engineering Program, Research Institute at Nationwide Children's Hospital, 700 Children's Drive, WB 4154, Columbus, OH, 43205, USA.
authors.forename: Eric D
authors.initials: ED
authors.lastname: Heuer
authors.affiliation: Tissue Engineering Program, Research Institute at Nationwide Children's Hospital, 700 Children's Drive, WB 4154, Columbus, OH, 43205, USA.
authors.forename: Lilamarie E
authors.initials: LE
authors.lastname: Moko
authors.affiliation: Tissue Engineering Program, Research Institute at Nationwide Children's Hospital, 700 Children's Drive, WB 4154, Columbus, OH, 43205, USA.
authors.forename: Shinka
authors.initials: S
authors.lastname: Miyamoto
authors.affiliation: Tissue Engineering Program, Research Institute at Nationwide Children's Hospital, 700 Children's Drive, WB 4154, Columbus, OH, 43205, USA.
authors.forename: Hideki
authors.initials: H
authors.lastname: Miyachi
authors.affiliation: The Heart Center, Nationwide Children's Hospital, Columbus, OH, USA.
authors.forename: Darren P
authors.initials: DP
authors.lastname: Berman
authors.affiliation: The Heart Center, Nationwide Children's Hospital, Columbus, OH, USA.
authors.forename: Sharon L
authors.initials: SL
authors.lastname: Cheatham
authors.affiliation: The Heart Center, Nationwide Children's Hospital, Columbus, OH, USA.
authors.forename: Joanne L
authors.initials: JL
authors.lastname: Chisolm
authors.affiliation: Tissue Engineering Program, Research Institute at Nationwide Children's Hospital, 700 Children's Drive, WB 4154, Columbus, OH, 43205, USA.|The Heart Center, Nationwide Children's Hospital, Columbus, OH, USA.|Department of Cardiothoracic Surgery, Nationwide Children's Hospital, Columbus, OH, USA.
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Shinoka
authors.affiliation: Tissue Engineering Program, Research Institute at Nationwide Children's Hospital, 700 Children's Drive, WB 4154, Columbus, OH, 43205, USA. christopher.breuer@nationwidechildrens.org.|Department of Pediatric Surgery, Nationwide Children's Hospital, Columbus, OH, USA. christopher.breuer@nationwidechildrens.org.
authors.forename: Christopher K
authors.initials: CK
authors.lastname: Breuer
authors.affiliation: The Heart Center, Nationwide Children's Hospital, Columbus, OH, USA.
authors.forename: John P
authors.initials: JP
authors.lastname: Cheatham
mesh_terms: D000818:Animals; D001705:Bioprosthesis; D001807:Blood Vessel Prosthesis; D019917:Blood Vessel Prosthesis Implantation / Q000009:adverse effects / Q000295:instrumentation*; D016026:Bone Marrow Transplantation; D002478:Cells, Cultured; D023421:Models, Animal; D010690:Phlebography; D011183:Postoperative Complications / Q000000981:diagnostic imaging / Q000473:pathology; D011474:Prosthesis Design; D034561:Sheep, Domestic; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D018084:Ultrasonography, Interventional; D014682:Vena Cava, Inferior / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery*
keywords: Angiography; IVUS; Intravascular ultrasound; Neotissue; Stenosis; TEVG; Tissue-engineered vascular graft