title: Application of Z-sinapinic matrix in peptide MALDI-MS analysis.
abstract: Since introduction of sinapinic acid (SA) and α-cyano-4-hydroxycinnamic acid as matrices, successful application of matrix-assisted laser desorption/ionization mass spectrometry started for protein/polypeptides. Both show some limitations in short peptide analysis because matrix clusters are quite abundant. Cinnamics currently used are E-cinnamics. Here, Z-SA as matrix for peptides is studied and compared with E-SA and α-cyano-4-hydroxycinnamic acid. Minor number of clusters is always observed in the low m/z region allowing the detection of short peptides. The results here described show that this novel matrix is a tool of choice for direct, rapid and sensitive detection of hydrophilic and hydrophobic peptides. Copyright © 2017 John Wiley & Sons, Ltd.
authors.affiliation: CIHDECAR-CONICET y Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon II, 3 piso, Ciudad Universitaria, 1428, Buenos Aires, Argentina.
authors.forename: M L
authors.initials: ML
authors.lastname: Salum
authors.affiliation: Consejo Nacional de lnvestigaciones Cientificas y Técnicas, Instituto de Nanobiotecnología (NANOBIOTEC), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113, Buenos Aires, Argentina.|Facultad de Farmacia y Bioquímica, Cátedra de Biotecnología, Universidad de Buenos Aires, Junín 956, 1113, Buenos Aires, Argentina.
authors.forename: S L
authors.initials: SL
authors.lastname: Giudicessi
authors.affiliation: CIHDECAR-CONICET y Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon II, 3 piso, Ciudad Universitaria, 1428, Buenos Aires, Argentina.
authors.forename: T
authors.initials: T
authors.lastname: Schmidt De León
authors.affiliation: Consejo Nacional de lnvestigaciones Cientificas y Técnicas, Instituto de Nanobiotecnología (NANOBIOTEC), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113, Buenos Aires, Argentina.|Facultad de Farmacia y Bioquímica, Cátedra de Biotecnología, Universidad de Buenos Aires, Junín 956, 1113, Buenos Aires, Argentina.
authors.forename: S A
authors.initials: SA
authors.lastname: Camperi
authors.affiliation: CIHDECAR-CONICET y Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon II, 3 piso, Ciudad Universitaria, 1428, Buenos Aires, Argentina.
authors.forename: R
authors.identifier: 0000-0003-0169-0173
authors.initials: R
authors.lastname: Erra-Balsells
mesh_terms: D000595:Amino Acid Sequence; D002934:Cinnamates / Q000737:chemistry; D003373:Coumaric Acids / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D057230:Limit of Detection; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D002934:Cinnamates; D003373:Coumaric Acids; D010455:Peptides; C029010:cinnamic acid; C007175:alpha-cyano-4-hydroxycinnamate; C073734:sinapinic acid
keywords: CHCA clusters; MALDI-MS; Z-sinapinic acid; peptides