title: Near-Infrared Fluorescent Ag2Se-Cetuximab Nanoprobes for Targeted Imaging and Therapy of Cancer.
abstract: Theranostic nanoprobes integrated with diagnostic imaging and therapy capabilities have shown great potential for highly effective tumor therapy by realizing imaging-guided drug delivery and tumor treatment. Developing novel high-performance nanoprobes is an important basis for tumor theranostic application. Here, near-infrared (NIR) fluorescent and low-biotoxicity Ag2Se quantum dots (QDs) have been coupled with cetuximab, a clinical antiepidermal growth factor receptor antibody drug for tumor therapy, via a facile bioconjugation strategy to prepare multifunctional Ag2Se-cetuximab nanoprobes. Compared with the Ag2Se QDs alone, the Ag2Se-cetuximab nanoprobes display faster and more enrichment at the site of orthotopic tongue cancer, and thus present better NIR fluorescence contrast between the tumor and the surrounding regions. At 24 h postinjection, the NIR fluorescence of Ag2Se-cetuximab nanoprobes at the tumor site is still easily detectable, whereas no fluorescence is observed for the Ag2Se QDs. Moreover, the Ag2Se-cetuximab nanoprobes have also significantly inhibited the tumor growth and improved the survival rate of orthotopic tongue cancer-bearing nude mice from 0% to 57.1%. Taken together, the constructed multifunctional Ag2Se-cetuximab nanoprobes have achieved combined targeted imaging and therapy of orthotopic tongue cancer, which may greatly contribute to the development of nanotheranostics.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, The Institute for Advanced Studies and Wuhan Institute of Biotechnology, Wuhan University, Wuhan, 430072, P. R. China.
authors.forename: Chun-Nan
authors.initials: CN
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Oral Biomedicine (Ministry of Education) and Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, P. R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, The Institute for Advanced Studies and Wuhan Institute of Biotechnology, Wuhan University, Wuhan, 430072, P. R. China.
authors.forename: Zhi-Quan
authors.initials: ZQ
authors.lastname: Tian
authors.affiliation: Key Laboratory of Oral Biomedicine (Ministry of Education) and Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Key Laboratory of Oral Biomedicine (Ministry of Education) and Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, P. R. China.
authors.forename: Wen-Qun
authors.initials: WQ
authors.lastname: Zhong
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, The Institute for Advanced Studies and Wuhan Institute of Biotechnology, Wuhan University, Wuhan, 430072, P. R. China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, The Institute for Advanced Studies and Wuhan Institute of Biotechnology, Wuhan University, Wuhan, 430072, P. R. China.
authors.forename: Zhi-Ling
authors.initials: ZL
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, The Institute for Advanced Studies and Wuhan Institute of Biotechnology, Wuhan University, Wuhan, 430072, P. R. China.
authors.forename: Dai-Wen
authors.initials: DW
authors.lastname: Pang
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D000068818:Cetuximab / Q000627:therapeutic use*; D003952:Diagnostic Imaging; D005260:Female; D005453:Fluorescence; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007259:Infrared Rays; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry*; D045663:Quantum Dots / Q000648:ultrastructure; D018036:Selenium Compounds / Q000737:chemistry* / Q000633:toxicity; D012834:Silver / Q000737:chemistry* / Q000633:toxicity; D012995:Solubility; D013499:Surface Properties; D016019:Survival Analysis; D000068936:Theranostic Nanomedicine; D013997:Time Factors; D014062:Tongue Neoplasms / Q000175:diagnosis* / Q000188:drug therapy* / Q000473:pathology; D016896:Treatment Outcome; D014867:Water / Q000737:chemistry
chemical_list: D018036:Selenium Compounds; D014867:Water; D012834:Silver; D000068818:Cetuximab
keywords: cancer therapy; cetuximab; targeted imaging; theranostic nanoprobes; tumor therapy