title: Diversity of the subspecies Bifidobacterium animalis subsp. lactis.
abstract: Strains of Bifidobacterium animalis subsp. lactis are well-known health-promoting probiotics used commercially. B. animalis subsp. lactis has been isolated from different sources, and little is known about animal isolates of this taxon. The aim of this study was to examine the genotypic and phenotypic diversity between B. animalis subsp. lactis strains different animal hosts including Cameroon sheep, Barbary sheep, okapi, mouflon, German shepard and to compare to BB12, food isolates and the collection strain DSM 10140. Ten strains of B. animalis subsp. lactis from different sources were characterised by phenotyping, fingerprinting, and multilocus sequence typing (MLST). Regardless of origin, MLST and phylogenetic analyses revealed a close relationship between strains of B. animalis subsp. lactis with commercial and animal origin with the exception of isolates from ovine cheese, mouflon and German Shepard dog. Moreover, isolates from dog and mouflon showed significant differences in fermentation profiles and peptide mass fingerprints (MALDI-TOF). Results indicated phenotypic and genotypic diversity among strains of B. animalis subsp. lactis.
authors.affiliation: Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences Prague, Kamýcka 129, Prague 6, 165 21, Czechia. Electronic address: bunesova@af.czu.cz.
authors.forename: Vera
authors.initials: V
authors.lastname: Bunesova
authors.affiliation: Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences Prague, Kamýcka 129, Prague 6, 165 21, Czechia; Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 4-Krč, 14 200, Czechia.
authors.forename: Jiri
authors.initials: J
authors.lastname: Killer
authors.affiliation: Department of Biochemistry and Microbiology, Institute of Chemical Technology Prague, Technická 5, 166 28, Prague, Czechia.
authors.forename: Barbora
authors.initials: B
authors.lastname: Javurkova
authors.affiliation: Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences Prague, Kamýcka 129, Prague 6, 165 21, Czechia.
authors.forename: Eva
authors.initials: E
authors.lastname: Vlkova
authors.affiliation: Department of Soil Science and Soil Protection, Czech University of Life Sciences Prague, Kamýcká 129, 165 21, Prague 6, Czechia.
authors.forename: Vaclav
authors.initials: V
authors.lastname: Tejnecky
authors.affiliation: Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences Prague, Kamýcka 129, Prague 6, 165 21, Czechia.
authors.forename: Sarka
authors.initials: S
authors.lastname: Musilova
authors.affiliation: Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences Prague, Kamýcka 129, Prague 6, 165 21, Czechia.
authors.forename: Vojtech
authors.initials: V
authors.lastname: Rada
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D000069982:Bifidobacterium animalis / Q000737:chemistry / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D005516:Food Microbiology; D014644:Genetic Variation; D005838:Genotype; D008322:Mammals / Q000382:microbiology*; D058889:Molecular Typing; D010641:Phenotype; D010802:Phylogeny; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
keywords: Bifidobacteria; Environment; Genotyping; Origin; Phenotyping