title: Comparative Transcriptomics Atlases Reveals Different Gene Expression Pattern Related toFusariumWilt Disease Resistance and Susceptibility in TwoVerniciaSpecies.
abstract: Vernicia fordii(tung oil tree) is a promising industrial crop. Unfortunately, the devastatingFusariumwilt disease has caused its great losses, while its sister species (Vernicia montana) is remarkably resistant to this pathogen. However, the genetic mechanisms underlying this difference remain largely unknown. We here generated comparative transcriptomic atlases for different stages ofFusarium oxysporuminfectedVerniciaroot. The transcriptomes ofV. fordiiandV. montanawere assembledde novoand contained 258,430 and 245,240 non-redundant transcripts with N50 values of 1776 and 2452, respectively. A total of 44,310 pairs of putative one-to-one orthologous genes were identified inVerniciaspecies. Overall, the vast majority of orthologous genes shared a remarkably similar expression mode. The expression patterns of a small set of genes were further validated by quantitative real-time PCR. Moreover, 157 unigenes whose expression significantly correlated between the two species were defined, and gene set enrichment analysis indicated roles in increased defense response and in jasmonic and salicylic acid signaling responses during pathogen attack. Co-expression network analysis further identified the 17 hub unigenes, such as the serine/threonine protein kinase D6PK, leucine-rich repeat receptor-like kinase (LRR-RLK), and EREBP transcription factor, which play essential roles in plant pathogen resistance. Intriguingly, the expression of most hub genes differed significantly betweenV. montanaandV. fordii. Based on our results, we propose a model to describe the major molecular reactions that underlie the defense responses of resistantV. montanatoF. oxysporum. These data represent a crucial step toward breeding more pathogen-resistantV. fordii.
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry Bejing, China.
authors.forename: Yicun
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry Bejing, China.
authors.forename: Hengfu
authors.initials: H
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry Bejing, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry Bejing, China.
authors.forename: Huiping
authors.initials: H
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry Bejing, China.
authors.forename: Qiyan
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry Bejing, China.
authors.forename: Yangdong
authors.initials: Y
authors.lastname: Wang
keywords: Fusarium wilt; Vernicia species; co-expression; comparative transcriptomics; resistance genes