title: Blood Meal Identification of the Mosquito (Diptera: Culicidae) Specimens Belong to Culex pipiens Complex that were Collected from Kayseri Province.
abstract: OBJECTIVE
  This study aimed to determine the host preferences in blood meal of specimens belonging to Culex pipiens complex.
  
  
  METHODS
  A total of 1284 female mosquitos were morphologically examined, and genomic DNA isolations were individually performed on 376 (28.4%) specimens that were determined to be Cx. pipiens complex. PCR was performed with primers to specifically amplify the avian and mammalian mitochondrial cytochrome b (mt-cytb) gene region. Amplicons were cloned, and the obtained plasmids were sequenced to determine host species.
  
  
  RESULTS
  Of 376 specimens, 148 (39.4%) were positive for the avian and/or mammalian blood meal. Among the positive specimens, 43, 98, and seven were determined to be positive for only mammalian, avian, and both avian and mammalian blood, respectively. Avian host preference in blood meal of the specimens belonging to Cx. pipiens was found to be significant. Of 15 avian blood positive isolates, nine, three, two, and one were designated as blood meal from avian species in Passeriformes, Accipitriformes, Columbiformes, and Strigiformes orders, respectively. While six, four, three, and two out of 15 mammalian blood-positive specimens were found to be positive for human, cattle, sheep, and dog blood, respectively.
  
  
  CONCLUSIONS
  Molecular data regarding the host preferences of the Cx. pipiens species complex in blood meal were revealed for the first time in Turkey with this study.
authors.affiliation: Erciyes Üniversitesi Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Kayseri, Türkiye. yildirima@erciyes.edu.tr.
authors.forename: Seval
authors.initials: S
authors.lastname: Korkmaz
authors.forename: Alparslan
authors.initials: A
authors.lastname: Yıldırım
authors.forename: Önder
authors.initials: Ö
authors.lastname: Düzlü
authors.forename: Arif
authors.initials: A
authors.lastname: Çiloğlu
authors.forename: Zuhal
authors.initials: Z
authors.lastname: Önder
authors.forename: Abdullah
authors.initials: A
authors.lastname: İnci
mesh_terms: D000818:Animals; D001717:Birds; D002417:Cattle; D003465:Culex / Q000145:classification / Q000502:physiology* / Q000821:virology; D004285:Dogs; D005247:Feeding Behavior; D005260:Female; D058507:Host Specificity; D006801:Humans; D007303:Insect Vectors / Q000145:classification / Q000502:physiology* / Q000821:virology; D012756:Sheep; D014421:Turkey; D014901:West Nile Fever / Q000635:transmission*