title: Evaluation of Two Methods for Determination of CD64 as a Diagnostic Marker of Infection in Critically Ill Adults.
abstract: Objectives. Diagnostic markers of infection have had little innovation over the last few decades. CD64, a marker expressed on the surface of neutrophils, may have utility for this purpose.Methods. This study was conducted in an adult intensive care unit (ICU) in São Paulo, Brazil, with 89 patients. We evaluated CD64 in patients with documented or clinically diagnosed infection (infection group) and controls (patients without any evidence of infection) by two different methodologies: method #1, an in house assay, and method #2, the commercial kit Leuko64 (Trillium Diagnostics).Results. CD64 displayed good discriminating power with a 91.2% sensitivity (95% CI 90.7-91.6%) for detecting infection. The commercial kit (Leuko64) demonstrated higher specificity (87.3%) compared with method #1 as well as better accuracy (88.8%).Conclusions. CD64 seems to be a promising marker of infection in the intensive care setting, with Leuko64 showing a slight advantage.
authors.affiliation: Intensive Care Unit, Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
authors.forename: Thiago
authors.identifier: 0000-0001-8005-0929
authors.initials: T
authors.lastname: Zinsly Sampaio Camargo
authors.affiliation: Instituto Israelita de Ensino e Pesquisa Albert Einstein, Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
authors.forename: Alexandre R
authors.initials: AR
authors.lastname: Marra
authors.affiliation: Clinical Laboratory, Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
authors.forename: Nydia Strachman
authors.initials: NS
authors.lastname: Bacal
authors.affiliation: Intensive Care Unit, Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Casaroto
authors.affiliation: Intensive Care Unit, Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
authors.forename: Lilian Moreira
authors.initials: LM
authors.lastname: Pinto
authors.affiliation: Clinical Laboratory, Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
authors.forename: Jacyr
authors.initials: J
authors.lastname: Pasternak
authors.affiliation: Instituto Israelita de Ensino e Pesquisa Albert Einstein, Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
authors.forename: Elivane da Silva
authors.initials: ED
authors.lastname: Victor
authors.affiliation: Division of Medical Practice, Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
authors.forename: Oscar Fernando Pavão
authors.initials: OF
authors.lastname: Dos Santos
authors.affiliation: Department of Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, IA, USA.
authors.forename: Michael B
authors.initials: MB
authors.lastname: Edmond
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood*; D016638:Critical Illness; D005260:Female; D006801:Humans; D007239:Infections / Q000097:blood* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000473:pathology; D017452:Receptors, IgG / Q000097:blood* / Q000302:isolation & purification
chemical_list: D015415:Biomarkers; C489017:FCGR1A protein, human; D017452:Receptors, IgG