title: Synthesis of silver nanoparticles (Ag NPs) for anticancer activities (MCF 7 breast and A549 lung cell lines) of the crude extract of Syzygium aromaticum.
abstract: In the present report, silver nanoparticles were synthesized using Piper nigrum extract for in vitro cytotoxicity efficacy against MCF-7 and HEP-2 cells. The silver nanoparticles (AgNPs) were formed within 20min and after preliminarily confirmation by UV-Visible spectroscopy (strong peak observed at ~441nm), they were characterized by using FT-IR and HR-TEM. The TEM images show spherical shape of biosynthesized AgNPs with particle size in the range 5-40nm while as compositional analysis were observed by EDAX. MTT assays were carried out for cytotoxicity of various concentrations of biosynthesized silver nanoparticles and Piper nigrum extract ranging from 10 to 100μg. The biosynthesized silver nanoparticles showed a significant anticancer activity against both MCF-7 and Hep-2 cells compared to Piper nigrum extract which was dose dependent. Our study thus revealed an excellent application of greenly synthesized silver nanoparticles using Piper nigrum. The study further suggested the potential therapeutic use of these nanoparticles in cancer study.
authors.affiliation: Department of Biotechnology, Vels University, Chennai, India.
authors.forename: K
authors.initials: K
authors.lastname: Venugopal
authors.affiliation: Department of Biotechnology, Vels University, Chennai, India.
authors.forename: H A
authors.initials: HA
authors.lastname: Rather
authors.affiliation: Department of Biotechnology, Vels University, Chennai, India.
authors.forename: K
authors.initials: K
authors.lastname: Rajagopal
authors.affiliation: Dept of Zoology, Nehru Memorial College, Puthanampatti-621007, Tiruchirappalli, Tamil Nadu, India.
authors.forename: M P
authors.initials: MP
authors.lastname: Shanthi
authors.affiliation: Dept of Virology, King Institute of Preventive Medicine and Research, Chennai 600032, India.
authors.forename: K
authors.initials: K
authors.lastname: Sheriff
authors.affiliation: Dept of Virology, King Institute of Preventive Medicine and Research, Chennai 600032, India.
authors.forename: M
authors.initials: M
authors.lastname: Illiyas
authors.affiliation: Dept of Virology, King Institute of Preventive Medicine and Research, Chennai 600032, India.
authors.forename: R A
authors.initials: RA
authors.lastname: Rather
authors.affiliation: Department of Biotechnology, Vels University, Chennai, India; Dept of Physics & Biochemistry, TUCAS, Thennangur-604408, Thiruvalluvar University, Serkadu, Vellore, India; UNESCO-UNISA AFNET in Nanosciences/Nanotechnology, College of Graduate Studies, University of South Africa, Muckleneuk Ridge, PO BOX 392, Pretoria, South Africa; Nanosciences African Network (NANO-AFNET), Materials Research Department, iThemba LABS-National Research Foundation (NRF), 1 Old Faure Road, Somerset West, PO BOX 722, Western Cape, South Africa. Electronic address: ammaaappa@gmail.com.
authors.forename: E
authors.initials: E
authors.lastname: Manikandan
authors.affiliation: Dept of Physics & Biochemistry, TUCAS, Thennangur-604408, Thiruvalluvar University, Serkadu, Vellore, India.
authors.forename: S
authors.initials: S
authors.lastname: Uvarajan
authors.affiliation: Dept of Zoology, UGC SAP-DSA-I, Sri Venkateswara University, Tirupati 517502, India.
authors.forename: M
authors.initials: M
authors.lastname: Bhaskar
authors.affiliation: UNESCO-UNISA AFNET in Nanosciences/Nanotechnology, College of Graduate Studies, University of South Africa, Muckleneuk Ridge, PO BOX 392, Pretoria, South Africa; Nanosciences African Network (NANO-AFNET), Materials Research Department, iThemba LABS-National Research Foundation (NRF), 1 Old Faure Road, Somerset West, PO BOX 722, Western Cape, South Africa.
authors.forename: M
authors.initials: M
authors.lastname: Maaza
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000627:therapeutic use*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000473:pathology; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D010936:Plant Extracts / Q000627:therapeutic use; D012834:Silver / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D020349:Surface Plasmon Resonance; D027842:Syzygium / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts; D012834:Silver
keywords: Anticancer activities; Cellines; Characterizations; Phytochemical synthesis; Silver nanoparticles