title: Cell laden hydrogel construct on-a-chip for mimicry of cardiac tissue in-vitro study.
abstract: Since the leading cause of death are cardiac diseases, engineered heart tissue (EHT) is one of the most appealing topics defined in tissue engineering and regenerative medicine fields. The importance of EHT is not only for heart regeneration but also for in vitro developing of cardiology. Cardiomyocytes could grow and commit more naturally in their microenvironment rather than traditional cultivation. Thus, this research tried to develop a set up on-a-chip to produce EHT based on chitosan hydrogel. Micro-bioreactor was hydrodynamically designed and simulated by COMSOL and produced via soft lithography process. Chitosan hydrogel was also prepared, adjusted, and assessed by XRD, FTIR and also its degradation rate and swelling ratio were determined. Finally, hydrogels in which mice cardiac progenitor cells (CPC) were loaded were injected into the micro-device chambers and cultured. Each EHT in every chamber was evaluated separately. Prepared EHTs showed promising results that expanded in them CPCs and work as an integrated syncytium. High cell density culture was the main accomplishment of this study.
authors.affiliation: Biomedical Engineering Group, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Ghiaseddin
authors.affiliation: Biomedical Engineering Group, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Pouri
authors.affiliation: Hematology Group, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
authors.affiliation: Biomedical Engineering Group, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Vasheghani-Farahani
authors.affiliation: Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Ahmadi Tafti
authors.affiliation: Biomedical Engineering Group, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
authors.forename: Sameereh
authors.initials: S
authors.lastname: Hashemi-Najafabadi
mesh_terms: D000818:Animals; D019149:Bioreactors; D049109:Cell Proliferation; D006321:Heart; D020100:Hydrogels; D056656:Lab-On-A-Chip Devices; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009206:Myocardium / Q000166:cytology; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering; D014961:X-Ray Diffraction
chemical_list: D020100:Hydrogels
keywords: Chitosan; Engineered heart tissue; Hydrogel; Micro-bioreactor; Microfluidic; Perfusion