title: Cross feeding of glucose metabolism byproducts ofEscherichia colihuman gut isolates and probiotic strains affect survival ofVibrio cholerae.
abstract: Vibrio choleraeconverts glucose into either acid or the neutral end product acetoin and its survival in carbohydrate enriched media is linked to the nature of the byproducts produced. It has been demonstrated in this study thatEscherichia colistrain isolated from the gut of healthy human volunteers and the commonly used probioticE. coliNissle strain that metabolize glucose to acidic byproducts drastically reduce the survival ofV. choleraestrains irrespective of their glucose sensitivity and acetoin production status. Accordingly,E. coliglucose transport mutants that produce lower amounts of acidic metabolites had little effect on the survival ofV. choleraein cocultures. Thus, cross feeding of byproducts of glucose metabolism by heterologous bacteria modulates the survival ofV. choleraein glucose rich medium suggesting that composition of the gut microbiota could influence the outcome ofV. choleraeinfection especially when glucose based ORS is administered.
authors.affiliation: CSIR-Indian Institute of Chemical Biology, 4 Raja SC Mullick Road, Kolkata, 700032 India.
authors.forename: Chirantana
authors.initials: C
authors.lastname: Sengupta
authors.affiliation: CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, 160036 India.
authors.forename: Manjula
authors.initials: M
authors.lastname: Ekka
authors.affiliation: CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, 160036 India.
authors.forename: Saurabh
authors.initials: S
authors.lastname: Arora
authors.affiliation: CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, 160036 India.
authors.forename: Prashant D
authors.initials: PD
authors.lastname: Dhaware
authors.affiliation: CSIR-Indian Institute of Chemical Biology, 4 Raja SC Mullick Road, Kolkata, 700032 India.
authors.forename: Rukhsana
authors.initials: R
authors.lastname: Chowdhury
authors.affiliation: CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, 160036 India.
authors.forename: Saumya
authors.initials: S
authors.lastname: Raychaudhuri
keywords: Cocultures; E. coli Nissle 1917; E. coli glucose transport mutants; V. cholerae survival