title: Effects of chlorogenic acid on intracellular calcium regulation in lysophosphatidylcholine-treated endothelial cells.
abstract: Lysophosphatidylcholine (LPC) is a major phospholipid component of oxidized low-density lipoprotein (ox-LDL) and is implicated in its atherogenic activity. This study investigated the effects of LPC on cell viability, intracellular calcium homeostasis, and the protective mechanisms of chlorogenic acid (CGA) in human umbilical vein endothelial cells (HUVECs). LPC increased intracellular calcium ([Ca2＋]i) by releasing Ca2＋from intracellular stores and via Ca2＋influx through store-operated channels (SOCs). LPC also increased the generation of reactive oxygen species (ROS) and decreased cell viability. The mRNA expression of Transient receptor potential canonical (TRPC) channel 1 was increased significantly by LPC treatment and suppressed by CGA. CGA inhibited LPC-induced Ca2＋influx and ROS generation, and restored cell viability. These results suggested that CGA inhibits SOC-mediated Ca2＋influx and ROS generation by attenuating TRPC1 expression in LPC-treated HUVECs. Therefore, CGA might protect endothelial cells against LPC injury, thereby inhibiting atherosclerosis. [BMB Reports 2017; 50(6): 323-328].
authors.affiliation: Department of Physiology, Keimyung University School of Medicine, Daegu 42601, Korea.
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Jung
authors.affiliation: Department of Physiology, Keimyung University School of Medicine, Daegu 42601, Korea.
authors.forename: Seung-Soon
authors.initials: SS
authors.lastname: Im
authors.affiliation: Department of Physiology, Keimyung University School of Medicine, Daegu 42601, Korea.
authors.forename: Dae-Kyu
authors.initials: DK
authors.lastname: Song
authors.affiliation: Department of Physiology, Keimyung University School of Medicine, Daegu 42601, Korea.
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Bae
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D002726:Chlorogenic Acid / Q000494:pharmacology*; D006706:Homeostasis / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D000074025:TRPA1 Cation Channel / Q000378:metabolism
chemical_list: D008077:Lipoproteins, LDL; D008244:Lysophosphatidylcholines; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D000074025:TRPA1 Cation Channel; C473350:TRPA1 protein, human; D002726:Chlorogenic Acid; D002118:Calcium