title: Quantitative proteomic analysis of gingival crevicular fluids from deciduous and permanent teeth.
abstract: OBJECTIVE
  Gingivitis commonly progresses to periodontitis in permanent dentition but rarely in deciduous teeth. Little is known about the biochemical differences between gingiva of deciduous and permanent teeth. Here, we compared the protein profiles of gingival crevicular fluids (GCF) from the gingiva of deciduous and permanent teeth.
  
  
  METHODS
  Forty children with mixed dentition (Hellman's dental age IIIA) were selected and GCF samples were collected from deciduous cuspids and central incisors in the maxilla. Pairs of GCF samples were labelled using isobaric tags to permit quantitative comparison of protein abundance in the samples using liquid chromatography-electron spray ionization-tandem mass spectrometry.
  
  
  RESULTS
  Sixty-two proteins were upregulated in deciduous teeth GCF and 54 in permanent teeth GCF. In particular, neutrophil-derived proteins, including myeloperoxidase and lactoferrin, were repeatedly higher in deciduous teeth GCF than in permanent teeth GCF. These differences were verified using ELISA (p < 0.01). In contrast, immunoglobulin components were upregulated in permanent teeth GCF.
  
  
  CONCLUSIONS
  Neutrophil-related proteins were enriched in deciduous teeth GCF and immunoglobulins in permanent teeth GCF. This suggests that neutrophil accumulation plays a protective role in innate immunity against bacterial infection in gingival tissue of deciduous teeth.
authors.affiliation: Department of Periodontology, Showa University School of Dentistry, Ohta-ku, Tokyo, Japan.|Division of Biological Chemistry, Department of Molecular Biology, Showa University School of Pharmacy, Shinagawa-ku, Tokyo, Japan.
authors.forename: Yumi
authors.identifier: http://orcid.org/0000-0002-9556-5316
authors.initials: Y
authors.lastname: Moriya
authors.affiliation: Division of Biological Chemistry, Department of Molecular Biology, Showa University School of Pharmacy, Shinagawa-ku, Tokyo, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Obama
authors.affiliation: Division of Biological Chemistry, Department of Molecular Biology, Showa University School of Pharmacy, Shinagawa-ku, Tokyo, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Aiuchi
authors.affiliation: Department of Pediatric Dentistry, Showa University School of Dentistry, Ohta-ku, Tokyo, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Sugiyama
authors.affiliation: Department of Pediatric Dentistry, Showa University School of Dentistry, Ohta-ku, Tokyo, Japan.
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Endo
authors.affiliation: Department of Periodontology, Showa University School of Dentistry, Ohta-ku, Tokyo, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Koide
authors.affiliation: Department of Periodontology, Showa University School of Dentistry, Ohta-ku, Tokyo, Japan.
authors.forename: Emiko
authors.initials: E
authors.lastname: Noguchi
authors.affiliation: Department of Periodontology, Showa University School of Dentistry, Ohta-ku, Tokyo, Japan.|Division of Biological Chemistry, Department of Molecular Biology, Showa University School of Pharmacy, Shinagawa-ku, Tokyo, Japan.
authors.forename: Motonori
authors.initials: M
authors.lastname: Ishizuka
authors.affiliation: Department of Pediatric Dentistry, Showa University School of Dentistry, Ohta-ku, Tokyo, Japan.
authors.forename: Mitsuko
authors.initials: M
authors.lastname: Inoue
authors.affiliation: Division of Biological Chemistry, Department of Molecular Biology, Showa University School of Pharmacy, Shinagawa-ku, Tokyo, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Itabe
authors.affiliation: Department of Periodontology, Showa University School of Dentistry, Ohta-ku, Tokyo, Japan.
authors.forename: Matsuo
authors.initials: M
authors.lastname: Yamamoto
mesh_terms: D002648:Child; D003818:Dentition, Mixed; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D008297:Male; D040901:Proteomics
keywords: deciduous dentition; gingival crevicular fluid; neutrophil function; permanent dentition; quantitative proteomics