title: Evaluation of Salivary Vitamin C and Catalase in HIV Positive and Healthy HIV Negative Control Group.
abstract: BACKGROUND
  Saliva is a complex oral biologic fluid secreted by major and minor salivary glands. Saliva has immunological, enzymatic and antioxidant defense mechanisms. Infection with human immunodeficiency virus (HIV) is a life-threatening disease.
  
  
  OBJECTIVE
  The aim of this study was to evaluate salivary vitamin C and catalase levels in HIV-positive patients in comparison to a healthy control group.
  
  
  METHODS
  Forty-nine HIV-infected individuals and 49 healthy subjects were selected. Five mL of unstimulated saliva was collected in 5 minutes using a sterilized Falcon tube with Navazesh method. Catalase and vitamin C levels were assessed by spectrophotometric assay. Data were analyzed with STATA 12.
  
  
  RESULTS
  Salivary catalase levels were 7.99±2.40 and 8.37±1.81 in the case and control groups, respectively. Catalase level was lower in the case group but the difference was not statistically significant (P=0.380). Salivary vitamin C levels in the case and control groups were 3.76±1.92 and 4.87±2.20, respectively (P=0.009).
  
  
  CONCLUSIONS
  HIV can alter salivary antioxidant capacity as well as vitamin C and catalase levels. Saliva may reflect serum antioxidative changes in these patients. Therefore, further research is necessary on salivary and serum oxidants and the antioxidant changes.
authors.affiliation: Dental Research Center and Research Center for Molecular Medicine, Department of Oral Medicine, Dental School, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Ahmadi-Motamayel
authors.affiliation: Department of Oral Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Samaneh
authors.initials: S
authors.lastname: Vaziri-Amjad
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Mohammad Taghi
authors.initials: MT
authors.lastname: Goodarzi
authors.affiliation: Research Center for Health Sciences and Department of Epidemiology & Biostatistics, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Jalal
authors.initials: J
authors.lastname: Poorolajal
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000032:analysis*; D001205:Ascorbic Acid / Q000032:analysis*; D018791:CD4 Lymphocyte Count; D002374:Catalase / Q000378:metabolism*; D005260:Female; D015658:HIV Infections / Q000276:immunology / Q000378:metabolism* / Q000821:virology; D064368:Healthy Volunteers; D006801:Humans; D008297:Male; D012463:Saliva / Q000737:chemistry* / Q000201:enzymology*; D013053:Spectrophotometry
chemical_list: D000975:Antioxidants; D002374:Catalase; D001205:Ascorbic Acid
keywords: HIV positive; Saliva; Spectrophotometric; antioxidant; catalase; saliva; vitamin C