title: Plasmon-induced photoelectrochemical biosensor for in situ real-time measurement of biotin-streptavidin binding kinetics under visible light irradiation.
abstract: We developed a localized surface plasmon-induced visible light-responsive photoelectrochemical (PEC) biosensor using a titanium dioxide (TiO2) photoelectrode loaded with gold nanoislands (AuNIs) for in situ real-time measurement of biotin-streptavidin association. As a proof of concept, self-assembled thiol-terminated biotin molecules bound on a AuNIs/TiO2photoelectrode were successfully utilized to explore the photocurrent response to streptavidin-modified gold nanoparticle (STA-AuNP) solutions. This plasmon-induced PEC biosensor is simple and easy to miniaturize. Additionally, the PEC biosensor achieves highly sensitive measurements under only visible light irradiation and prevents the UV-induced damage of samples. Furthermore, a novel approach has been proposed to realize the real-time monitoring of biotin-STA binding affinities and kinetics by analyzing the PEC sensing characteristics. This PEC biosensor and novel analysis method could provide a new approach for the specific electrical detection and real-time kinetic measurements for clinical diagnostics and drug development.
authors.affiliation: Research Institute for Electronic Science, Hokkaido University, Sapporo 001-0021, Japan.
authors.forename: Jingchun
authors.initials: J
authors.lastname: Guo
authors.affiliation: Research Institute for Electronic Science, Hokkaido University, Sapporo 001-0021, Japan.
authors.forename: Tomoya
authors.initials: T
authors.lastname: Oshikiri
authors.affiliation: Research Institute for Electronic Science, Hokkaido University, Sapporo 001-0021, Japan.
authors.forename: Kosei
authors.initials: K
authors.lastname: Ueno
authors.affiliation: Research Institute for Electronic Science, Hokkaido University, Sapporo 001-0021, Japan.
authors.forename: Xu
authors.initials: X
authors.lastname: Shi
authors.affiliation: Research Institute for Electronic Science, Hokkaido University, Sapporo 001-0021, Japan; Department of Applied Chemistry & Institute of Molecular Science, National Chiao Tung University, Hsinchu 30010, Taiwan. Electronic address: misawa@es.hokudai.ac.jp.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Misawa
mesh_terms: D015374:Biosensing Techniques; D001710:Biotin / Q000737:chemistry*; D055664:Electrochemical Techniques; D006046:Gold; D007700:Kinetics; D008027:Light; D053768:Metal Nanoparticles; D019809:Streptavidin / Q000737:chemistry*
chemical_list: D001710:Biotin; D006046:Gold; D019809:Streptavidin
keywords: Kinetic analysis; Localized surface plasmon; Photoelectrochemical biosensor; Visible light-response