title: Identification of potential antioxidant indices by biogenic gold nanoparticles in hyperglycemic Wistar rats.
abstract: Oxidative stress is a crucial factor in diabetes, where the abnormal metabolic ambience leads to hyperglycemia resulting in the onset of several vascular complications. Under homeostasis, innate antioxidants efficiently inhibit the oxidative stress, thereby restrain further progression of diabetes. In the present study, a potential antioxidant marker was identified from hepatic tissue of diabetic Wistar rats after oral administration of biogenic gold nanoparticles (GNPs). Diabetic animals treated with GNPs showed increase in insulin level and subsequently reduced the concentration of blood glucose level to normal. Further, GNPs favoured to retain the hepatic enzymatic markers, serum lipid levels and followed by renal biochemical profile in the rats. In addition, GNPs treated rats displayed an elevated level of lipid peroxidation, superoxide dismutase, glutathione peroxidase, and catalase enzymatic activity. Consequently, GNPs treated rats showed diminished level of histological injury in the hepatic, renal, and pancreatic tissues. Taken together, these results suggested that among the several antioxidant enzymes, catalase elucidated the highest area under curve (AUC) with 0.80 accomplished by receiver operating characteristic (ROC) curve. Collectively, our findings enlighten that GNPs treated rat able to alleviate the hyperglycemic condition due to the enzymatic activity of catalase.
authors.affiliation: Department of Biomedical Sciences, School of Biosciences and Technology, VIT University, Vellore 632014, India.
authors.forename: Manimegalai
authors.initials: M
authors.lastname: Sengani
authors.affiliation: Department of Biomedical Sciences, School of Biosciences and Technology, VIT University, Vellore 632014, India. Electronic address: sdevirajeswari@gmail.com.
authors.forename: Devi Rajeswari
authors.initials: DR
authors.lastname: V
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001786:Blood Glucose / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006046:Gold / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology; D006943:Hyperglycemia / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D018384:Oxidative Stress; D010936:Plant Extracts / Q000008:administration & dosage* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D064028:Tracheophyta / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D007328:Insulin; D010936:Plant Extracts; D006046:Gold; D002374:Catalase; D013482:Superoxide Dismutase
keywords: Antioxidants; Catalase; Couroupita guianensis; Diabetes; Gold nanoparticles