title: Characterization of Developmental Immature Fiber ( im) Mutant and Texas Marker-1 (TM-1) Cotton Fibers Using Attenuated Total Reflection Fourier Transform Infrared (ATR FT-IR) Spectroscopy.
abstract: The immature fiber ( im) mutant is one type of cotton fiber mutant with unique characteristics of non-fluffy cotton bolls. Compared to its near-isogenic wild type Texas Marker-1 (TM-1), im fiber has a thin secondary cell wall and is less mature. In this work, we applied the previously proposed principal component analysis (PCA) and simple algorithms to analyze the attenuated total reflection Fourier transform infrared (ATR FT-IR) spectra of developmental im and TM-1 fibers. The results from these approaches could not effectively and consistently indicate the inherent difference between TM-1 and im fibers at the same developmental stage. The difference between TM-1 and corresponding im fibers was detected when comparing the normalized intensity variations of the 730 cm-1bands. The 730 cm-1band intensities in developmental im fibers are temporally lower than those in developmental TM-1 fibers although they became similar when the TM-1 and im fibers are fully mature. The observation might imply the likelihood of temporal reduction of amorphous regions in developmental im fibers rather than in developmental TM-1 fibers.
authors.affiliation: 1 USDA, ARS, Cotton Structure & Quality Research Unit, Southern Regional Research Center (SRRC), New Orleans, LA, USA.
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: 2 USDA, ARS, Cotton Fiber Bioscience Research Unit, Southern Regional Research Center (SRRC), New Orleans, LA, USA.
authors.forename: Hee-Jin
authors.initials: HJ
authors.lastname: Kim
mesh_terms: D000465:Algorithms; D015415:Biomarkers / Q000032:analysis / Q000737:chemistry*; D002482:Cellulose / Q000032:analysis / Q000737:chemistry* / Q000145:classification; D032561:Cotton Fiber / Q000145:classification*; D003368:Gossypium / Q000737:chemistry / Q000145:classification; D010641:Phenotype; D025341:Principal Component Analysis; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D015415:Biomarkers; D002482:Cellulose
keywords: ATR; FT-IR; Fourier transform infrared spectroscopy; algorithm analysis; attenuated total reflection; cellulose; fiber secondary wall biosynthesis; im mutant; principal component analysis