title: Efficacy of Korean Multipurpose Contact Lens Disinfecting Solutions againstAcanthamoeba castellanii.
abstract: Acanthamoebakeratitis has been increasing in recent years. Main risk factors are contact lens wear and their cleaning solutions. Most contact lens wearers use multipurpose disinfecting solutions (MPDS) for cleansing and disinfecting microorganisms because of its convenience. We determined amoebicidal effects of MPDS made in Korea and their cytotoxicity on human corneal epithelium cells. Fifteen commercial MPDS (A to O) were tested for their amoebicidal effects onAcanthamoeba castellaniitrophozoites and cysts by using a most probable number (MPN) technique. Among them, 7 kinds of MPDS showed little or no amoebicidal effects for 24 hr exposure. Solutions A, B, G, H, L, and O showed positive amoebicidal effects, and solutions M and N killed almost all trophozoites and cysts after 24 hr exposure. However, 50%-N solution showed 56% cytotoxicity on human corneal epithelial cells within 4 hr exposure, and 50%-O solution also showed 62% cytotoxicity on human cells within 4 hr exposure. Solution A did not show any cytotoxicity on human cells. These results revealed that most MPDS made in Korea were ineffective to killAcanthamoeba. The solutions having amoebicidal activity also showed high levels of cytotoxicity on human corneal epithelial cells. New formulations for improved MPDS that are amoebicidal but safe for host cells are needed to preventAcanthamoebakeratitis.
authors.affiliation: Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul 02447, Korea.
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Moon
authors.affiliation: Department of Parasitology, Dong-A University College of Medicine, Busan 49201, Korea.
authors.forename: Hye-Ryun
authors.initials: HR
authors.lastname: Park
authors.affiliation: Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul 02447, Korea.
authors.forename: Fu-Shi
authors.initials: FS
authors.lastname: Quan
authors.affiliation: Department of Parasitology, Dong-A University College of Medicine, Busan 49201, Korea.
authors.forename: Hyun-Hee
authors.initials: HH
authors.lastname: Kong
mesh_terms: D048248:Acanthamoeba castellanii / Q000187:drug effects* / Q000502:physiology*; D018639:Contact Lens Solutions / Q000494:pharmacology* / Q000633:toxicity; D004847:Epithelial Cells / Q000187:drug effects; D006801:Humans; D007723:Korea; D050296:Microbial Viability
chemical_list: D018639:Contact Lens Solutions
keywords: Acanthamoeba castellanii; cytotoxicity; multipurpose disinfecting solution (MPDS)