title: Enhanced microalgal biomass and lipid production from a consortium of indigenous microalgae and bacteria present in municipal wastewater under gradually mixotrophic culture conditions.
abstract: The goal of this study was to investigate the influences of gradually mixotrophic culture conditions on microalgal biomass and lipid production by a consortium of indigenous microalgae and bacteria present in raw municipal wastewater. Lab-scale photobioreactors containing the consortium were operated in repeated batch mode. Initial cultivation (phase I) was performed using only the municipal wastewater, then 10% and 25% of the reactor volumes were replaced with the effluent from a sewage sludge fermentation system producing volatile fatty acids (SSFV) at the beginnings of phase II and phase III, respectively. The highest biomass productivity (117.1±2.7mg/L/d) was attained during phase II, but the lipid productivity (17.2±0.2mg/L/d) was attained during phase III. The increase in the effluent from the SSFV influenced microalgal diversity with a preference for Chlorella sp., but bacterial diversity increased significantly during phase III.
authors.affiliation: School of Environmental Science and Engineering, Pohang University of Science and Technology, 77, Cheongam-ro, Nam-gu, Pohang, Gyeongbuk 37673, Republic of Korea; Bioenergy Research Center, Pohang University of Science and Technology, 77, Cheongam-ro, Nam-gu, Pohang, Gyeongbuk 37673, Republic of Korea.
authors.forename: Hyun Uk
authors.initials: HU
authors.lastname: Cho
authors.affiliation: School of Earth Science and Environmental Engineering, Gwang-ju Institute of Science and Technology, 123, Cheomdangwagi-ro, Buk-gu, Gwang-ju 61005, Republic of Korea.
authors.forename: Young Mo
authors.initials: YM
authors.lastname: Kim
authors.affiliation: School of Environmental Science and Engineering, Pohang University of Science and Technology, 77, Cheongam-ro, Nam-gu, Pohang, Gyeongbuk 37673, Republic of Korea; Bioenergy Research Center, Pohang University of Science and Technology, 77, Cheongam-ro, Nam-gu, Pohang, Gyeongbuk 37673, Republic of Korea; Department of Chemical Engineering, Pohang University of Science and Technology, 77, Cheongam-ro, Nam-gu, Pohang, Gyeongbuk 37673, Republic of Korea; Division of Advanced Nuclear Engineering, Pohang University of Science and Technology, 77, Cheongam-ro, Nam-gu, Pohang, Gyeongbuk 37673, Republic of Korea. Electronic address: jmpark@postech.ac.kr.
authors.forename: Jong Moon
authors.initials: JM
authors.lastname: Park
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D061249:Batch Cell Culture Techniques / Q000379:methods*; D018533:Biomass; D018929:Cell Culture Techniques / Q000379:methods*; D002708:Chlorella / Q000254:growth & development / Q000378:metabolism; D058645:Denaturing Gradient Gel Electrophoresis; D005232:Fatty Acids, Volatile / Q000032:analysis; D005285:Fermentation; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology; D062065:Wastewater / Q000382:microbiology*
chemical_list: D005232:Fatty Acids, Volatile; D008055:Lipids; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D062065:Wastewater
keywords: Fatty acids; Microalgal biomass; Microalgal-bacterial consortium; Mixotrophic culture; Volatile fatty acids (VFAs)