title: Quantitative SPM Analysis Involving an Adaptive Template May Be Easily Applied to [18F]FDG PET Images of the Rat Brain.
abstract: The Statistical Parametric Mapping (SPM) software is frequently used for the quantitative analysis of patients' brain images obtained from 2-deoxy-2-[18F]fluoro-D-glucose positron emission tomography ([18F]FDG PET). However, its adaptation to small animals is difficult, particularly for the initial step of spatial normalization which requires a specific brain anatomical template. This study was aimed at determining whether SPM analysis can be applied to rat, and more specifically to the lithium-pilocarpine model of epilepsy, by using an adaptive template. This template developed for PET clinical imaging is constructed from a block matching algorithm.
  
  
  
  SPM analysis of brain [18F]FDG PET images from Sprague-Dawley rats was used with the block matching (BM) adaptive template for the detection of brain abnormalities (1) artificially inserted within the initially normal brain images of 10 rats (50 % decrease in signal intensity within 40 spheres of 0.5 to 1.0 mm in diameter) and (2) occurring at 4 h (n = 16), 48 h (n = 15), and 8 days (n = 13) after lithium-pilocarpine treatment.
  
  
  
  Concordant positive clusters were documented for all inserted abnormalities, whereas no aberrant clusters were documented in remote brain areas. Positive clusters were also detected on sites known to be involved in the epileptogenesis process of the lithium-pilocarpine model (piriform and entorhinal cortex, hippocampus), with the expected time-specific changes involving an early hypermetabolism followed by a severe hypometabolism and a subsequent partial recovery.
  
  
  
  A quantitative SPM analysis of brain [18F]FDG PET images may be applied to the monitoring of rat brain function when using an adaptive BM template.
authors.affiliation: NANCYCLOTEP-Experimental Imaging Platform, F-5400, Nancy, France. sylvain.poussier@univ-lorraine.fr.|INSERM, IADI U947 and Lorraine University, F-5400, Nancy, France. sylvain.poussier@univ-lorraine.fr.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Poussier
authors.affiliation: NANCYCLOTEP-Experimental Imaging Platform, F-5400, Nancy, France.|Department of Nuclear Medicine, University Hospital, F-5400, Nancy, France.
authors.forename: Fatiha
authors.initials: F
authors.lastname: Maskali
authors.affiliation: Department of Neurology, University Hospital, F-5400, Nancy, France.
authors.forename: Gaelle
authors.initials: G
authors.lastname: Vexiau
authors.affiliation: NANCYCLOTEP-Experimental Imaging Platform, F-5400, Nancy, France.|INSERM, IADI U947 and Lorraine University, F-5400, Nancy, France.|Department of Nuclear Medicine, University Hospital, F-5400, Nancy, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Verger
authors.affiliation: NANCYCLOTEP-Experimental Imaging Platform, F-5400, Nancy, France.
authors.forename: Henri
authors.initials: H
authors.lastname: Boutley
authors.affiliation: NANCYCLOTEP-Experimental Imaging Platform, F-5400, Nancy, France.|Department of Nuclear Medicine, University Hospital, F-5400, Nancy, France.|INSERM U1116 and Lorraine University, F-5400, Nancy, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Karcher
authors.affiliation: Department of Neurology, University Hospital, F-5400, Nancy, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Raffo
authors.affiliation: NANCYCLOTEP-Experimental Imaging Platform, F-5400, Nancy, France.|Department of Nuclear Medicine, University Hospital, F-5400, Nancy, France.|INSERM U1116 and Lorraine University, F-5400, Nancy, France.
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Marie
mesh_terms: D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging*; D019788:Fluorodeoxyglucose F18 / Q000737:chemistry*; D021621:Imaging, Three-Dimensional; D008297:Male; D049268:Positron-Emission Tomography; D017207:Rats, Sprague-Dawley; D013223:Statistics as Topic
chemical_list: D019788:Fluorodeoxyglucose F18
keywords: Brain PET; Epilepsy; FDG; Spatial normalization; Statistical Parametric Mapping