title: [Effect of methamphetamine exposure on S-nitrosylation of protein disulphide isomerase in PC12 cells].
abstract: To study the effect of methamphetamine (METH) exposure on S-nitrosylation of protein disulphide isomerase and the neurotoxicity of METH in PC12 cells.
  
  
  
  PC12 cells were exposed to different concentrations of METH, and the cell viability was assessed using the cell-counting kit-8. PC12 cells exposed to METH in the presence of the NOS inhibitor N-nitro-L-arginine (L-NNA) were examined for cell viability and S-nitrosylation of protein disulphide isomerase using the biotin-switch method, and the changes in cell morphology were examined with HE staining.
  
  
  
  METH exposure obviously decreased the cell viability and increased S-nitrosylation of protein disulphide isomerase, and the effect of METH was obviously inhibited by L-NNA treatment.
  
  
  
  METH can cause obvious neurotoxicity and promote S-nitrosylation of protein disulphide isomerase in PC12 cells.
authors.affiliation: School of Medicine and Healthcare, Shunde Polytechnic, Foshan 528000, China.E-mail: 13512783934@163.com.
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Wu
authors.forename: Ai-Feng
authors.initials: AF
authors.lastname: Wang
authors.forename: Ping-Ming
authors.initials: PM
authors.lastname: Qiu
mesh_terms: D000818:Animals; D002452:Cell Count; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008694:Methamphetamine / Q000494:pharmacology / Q000633:toxicity*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D019335:Nitroarginine / Q000494:pharmacology; D016716:PC12 Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D019704:Protein Disulfide-Isomerases / Q000378:metabolism*; D051381:Rats
chemical_list: D004791:Enzyme Inhibitors; D019335:Nitroarginine; D008694:Methamphetamine; D019001:Nitric Oxide Synthase; D019704:Protein Disulfide-Isomerases