title: Development of 15 polymorphic microsatellite markers forFicus virens(Moraceae).
abstract: OBJECTIVE
  Ficus virens(Moraceae) is distributed widely in South and Southeast Asia, Melanesia, and northern Australia, and it is also cultivated outside its original northern range limit in southwestern China. Therefore, the species is well suited to explore the mechanism of range limits ofFicusspecies. However, little is known about its genetic background.
  
  
  RESULTS
  Fifteen polymorphic microsatellite markers were developed using the biotin-streptavidin capture method. Polymorphism was tested in 85F. virensindividuals sampled from three populations. The number of alleles ranged from three to 17. The observed and expected heterozygosity of each population varied from 0.0667 to 0.9286 and 0.0650 to 0.8890, respectively. Cross-species amplification was also carried out in eight otherFicusspecies.
  
  
  CONCLUSIONS
  These 15 markers will be valuable for studying the genetic variation and population structure ofF. virensand relatedFicusspecies.
authors.affiliation: Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, Sichuan 637002, People's Republic of China; Ecological Security and Protection Key Laboratory of Sichuan Province, Mianyang Normal University, Sichuan 621000, People's Republic of China.
authors.forename: Rong-Hua
authors.initials: RH
authors.lastname: Fu
authors.affiliation: Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, Sichuan 637002, People's Republic of China.
authors.forename: Yun-Xiang
authors.initials: YX
authors.lastname: Li
authors.affiliation: Ecological Security and Protection Key Laboratory of Sichuan Province, Mianyang Normal University, Sichuan 621000, People's Republic of China.
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.affiliation: Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, Sichuan 637002, People's Republic of China.
authors.forename: Qiu-Mei
authors.initials: QM
authors.lastname: Quan
keywords: Ficus virens; Moraceae; cross-amplification; genetic diversity; microsatellite; southwest China