title: Phytochemical and Antinociceptive, Anti-Inflammatory, and Antioxidant Studies ofSmilax larvata(Smilacaceae).
abstract: The tea of aerial parts ofSmilax larvataGriseb. (Smilacaceae) has been ethnopharmacologically used in Southern Brazil due to its anti-inflammatory action. In this study, ethanolic and organic extracts from aerial parts ofS. larvatawere phytochemically and pharmacologically characterized. The phytochemical analysis of EtOAc extract ofS. larvatarevealed the presence of three flavonoids, drabanemoroside, kaempferol 3-O-α-L-rhamnopyranosyl(1→2)-α-L-rhamnopyranoside, and kaempferol, the first two being isolated for the first time in this genus, two phenolic compoundsp-hydroxybenzoic acid andp-coumaric acid, and alkaloids. In vitro assays demonstrated a potential antioxidant property of SLG. The treatment with SLG induced a significant reduction of the formalin-evoked flinches in rats, an effect reversed by opioid antagonist naloxone. Treatment with SLG also induced a significant increase in the hot plate latency and a decrease of intestinal motility by 45%. No effect was observed over nociceptive responses induced by a TRPA1 agonist mustard oil or over acetic acid-induced writhing in mice. Together, our data suggested that SLG has an in vivo antinociceptive effect, which seems to be associated with the opioid system activation. These findings support previous claims of medical use ofSmilax larvatain the treatment of pain conditions.
authors.affiliation: Pharmacotechnical Laboratory, Department of Pharmacy, Universidade Federal do Paraná, 80.210-170 Curitiba, PR, Brazil.
authors.forename: Beatriz Cristina Konopatzki
authors.identifier: 0000-0002-2766-2368
authors.initials: BC
authors.lastname: Hirota
authors.affiliation: Pharmacotechnical Laboratory, Department of Pharmacy, Universidade Federal do Paraná, 80.210-170 Curitiba, PR, Brazil.
authors.forename: Cristiane da Silva
authors.identifier: 0000-0002-5170-5727
authors.initials: CD
authors.lastname: Paula
authors.affiliation: Phytochemistry Laboratory, Department of Pharmacy, Universidade Federal do Paraná, 80.210-170 Curitiba, PR, Brazil.
authors.forename: Vinícius Bednarczuk
authors.identifier: 0000-0001-7821-7742
authors.initials: VB
authors.lastname: de Oliveira
authors.affiliation: Laboratory of Pharmacology of Pain, Department of Pharmacology, Universidade Federal do Paraná, 80.210-170 Curitiba, PR, Brazil.
authors.forename: Joice Maria
authors.initials: JM
authors.lastname: da Cunha
authors.affiliation: Laboratory of Pharmacology of Pain, Department of Pharmacology, Universidade Federal do Paraná, 80.210-170 Curitiba, PR, Brazil.
authors.forename: Anne Karoline
authors.initials: AK
authors.lastname: Schreiber
authors.affiliation: Phytochemistry Laboratory, Department of Pharmacy, Universidade Federal do Paraná, 80.210-170 Curitiba, PR, Brazil; NMR Center, Chemistry Department, Universidade Federal do Paraná, 80.210-170 Curitiba, PR, Brazil.
authors.forename: Fernanda Maria Marins
authors.initials: FM
authors.lastname: Ocampos
authors.affiliation: NMR Center, Chemistry Department, Universidade Federal do Paraná, 80.210-170 Curitiba, PR, Brazil.
authors.forename: Anderson
authors.initials: A
authors.lastname: Barison
authors.affiliation: Phytochemistry Laboratory, Department of Pharmacy, Universidade Federal do Paraná, 80.210-170 Curitiba, PR, Brazil.
authors.forename: Obdulio Gomes
authors.initials: OG
authors.lastname: Miguel
authors.affiliation: Pharmacotechnical Laboratory, Department of Pharmacy, Universidade Federal do Paraná, 80.210-170 Curitiba, PR, Brazil.
authors.forename: Marilis Dallarmi
authors.initials: MD
authors.lastname: Miguel