title: Nioella sediminis sp. nov., isolated from surface sediment and emended description of the genus Nioella.
abstract: A bacterial strain, designated JS7-11T, was isolated from the surface sediment of the Jiulong River and characterized using a polyphasic taxonomic approach. Cells of JS7-11T were Gram-staining-negative, non-motile and short-rod-shaped. Growth was observed at 10-40 °C (optimum 25-30 °C), at pH 6-8 (optimum 7) and in 1-12 % NaCl (optimum 3-4 %, w/v). The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that JS7-11T and Nioella nitratireducens SSW136T formed an independent cluster, sharing 97.9 % similarity between the two strains, and less than 96.0 % similarities with other type strains of members of the family Rhodobacteraceae. The major fatty acids were summed feature 8 and C16 : 0. The quinone was identified as Q-10. The polar lipids comprised phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and two unidentified phospholipids. The DNA G+C content was 63.4 mol%. The digital DNA-DNA hybridization and average nucleotide identity values between JS7-11T and N. nitratireducens SSW136T were 22.1 and 79.4 %, respectively. The results of phenotypic, phylogenetic and chemotaxonomic analyses clearly indicated that JS7-11T represents a novel species within the genus Nioella, for which the name Nioellasediminis sp. nov. is proposed. The type strain of the novel species is JS7-11T (=MCCC 1A00710T=KCTC 42144T).
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, State Oceanic Administration, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Fujian Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA, Xiamen 361005, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, State Oceanic Administration, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Fujian Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA, Xiamen 361005, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, State Oceanic Administration, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Fujian Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA, Xiamen 361005, PR China.
authors.forename: Qiliang
authors.initials: Q
authors.lastname: Lai
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, State Oceanic Administration, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Fujian Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA, Xiamen 361005, PR China.
authors.forename: Chunming
authors.initials: C
authors.lastname: Dong
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, State Oceanic Administration, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Fujian Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA, Xiamen 361005, PR China.
authors.forename: Zongze
authors.initials: Z
authors.lastname: Shao
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D045483:Rivers / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2