title: Evaluation of sample extraction methods for minimizing hematocrit effect on whole blood analysis with volumetric absorptive microsampling.
abstract: OBJECTIVE
  Volumetric absorptive microsampler (VAMS) was designed to sample a fixed volume of blood regardless of the hematocrit (HCT) levels. Model compounds with a wide range of hydrophobicity were evaluated for their extraction recoveries from VAMS dried blood samples.
  
  
  RESULTS
  For the highly hydrophobic compounds, recoveries with methanol or methanol/acetonitrile extraction were higher compared with using the aqueous mixture of methanol or acetonitrile. Extraction with methanol/acetonitrile (1:1) yielded more consistent recovery across the HCT range of 20-70% than using methanol alone, with good linearity, accuracy and precision achieved from 1 to 2000 ng/ml.
  
  
  CONCLUSIONS
  An organic solvent sample preparation approach was developed to optimize extraction recovery and minimize the HCT effect on the analysis of VAMS dried blood samples.
authors.affiliation: Drug Metabolism & Pharmacokinetics, Vertex Pharmaceuticals, Inc. Boston, MA 02210, USA.
authors.forename: Zhengqi
authors.initials: Z
authors.lastname: Ye
authors.affiliation: Drug Metabolism & Pharmacokinetics, Vertex Pharmaceuticals, Inc. Boston, MA 02210, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Gao
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D001800:Blood Specimen Collection; D002853:Chromatography, Liquid; D059788:Dried Blood Spot Testing / Q000379:methods; D006400:Hematocrit; D006403:Hematologic Tests; D006801:Humans; D016014:Linear Models; D000432:Methanol / Q000737:chemistry; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D013048:Specimen Handling / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D000097:Acetonitriles; D012997:Solvents; D000432:Methanol; C032159:acetonitrile
keywords: hematocrit; microsampling; volumetric absorptive microsampler