title: Red Wine and Pomegranate Extracts Suppress Cured Meat Promotion of Colonic Mucin-Depleted Foci in Carcinogen-Induced Rats.
abstract: Processed meat intake is carcinogenic to humans. We have shown that intake of a workshop-made cured meat with erythorbate promotes colon carcinogenesis in rats. We speculated that polyphenols could inhibit this effect by limitation of endogenous lipid peroxidation and nitrosation. Polyphenol-rich plant extracts were added to the workshop-made cured meat and given for 14 days to rats and 100 days to azoxymethane-induced rats to evaluate the inhibition of preneoplastic lesions. Colons of 100-d study were scored for precancerous lesions (mucin-depleted foci, MDF), and biochemical end points of peroxidation and nitrosation were measured in urinary and fecal samples. In comparison with cured meat-fed rats, dried red wine, pomegranate extract, α-tocopherol added at one dose to cured meat and withdrawal of erythorbate significantly decreased the number of MDF per colon (but white grape and rosemary extracts did not). This protection was associated with the full suppression of fecal excretion of nitrosyl iron, suggesting that this nitroso compound might be a promoter of carcinogenesis. At optimized concentrations, the incorporation of these plant extracts in cured meat might reduce the risk of colorectal cancer associated with processed meat consumption.
authors.affiliation: a INRA UMR1331, TOXALIM (Research Center in Food Toxicology), Université de Toulouse, ENVT, INP , Toulouse , France.
authors.forename: Nadia M
authors.initials: NM
authors.lastname: Bastide
authors.affiliation: a INRA UMR1331, TOXALIM (Research Center in Food Toxicology), Université de Toulouse, ENVT, INP , Toulouse , France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Naud
authors.affiliation: b IFIP-Institut du Porc , Paris , France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Nassy
authors.affiliation: b IFIP-Institut du Porc , Paris , France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Vendeuvre
authors.affiliation: a INRA UMR1331, TOXALIM (Research Center in Food Toxicology), Université de Toulouse, ENVT, INP , Toulouse , France.
authors.forename: Sylviane
authors.initials: S
authors.lastname: Taché
authors.affiliation: a INRA UMR1331, TOXALIM (Research Center in Food Toxicology), Université de Toulouse, ENVT, INP , Toulouse , France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Guéraud
authors.affiliation: c Department of Food and Nutritional Sciences , University of Reading , Whiteknights , UK.
authors.forename: Ditte A
authors.initials: DA
authors.lastname: Hobbs
authors.affiliation: c Department of Food and Nutritional Sciences , University of Reading , Whiteknights , UK.
authors.forename: Gunter G
authors.initials: GG
authors.lastname: Kuhnle
authors.affiliation: a INRA UMR1331, TOXALIM (Research Center in Food Toxicology), Université de Toulouse, ENVT, INP , Toulouse , France.
authors.forename: Denis E
authors.initials: DE
authors.lastname: Corpet
authors.affiliation: a INRA UMR1331, TOXALIM (Research Center in Food Toxicology), Université de Toulouse, ENVT, INP , Toulouse , France.
authors.forename: Fabrice H F
authors.initials: FH
authors.lastname: Pierre
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000652:urine; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000517:prevention & control; D005243:Feces; D005752:Gastric Mucins / Q000378:metabolism; D015227:Lipid Peroxidation; D029561:Lythraceae / Q000737:chemistry*; D008297:Male; D008460:Meat / Q000009:adverse effects* / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology*; D011230:Precancerous Conditions / Q000139:chemically induced / Q000178:diet therapy*; D011916:Rats, Inbred F344; D014920:Wine; D024502:alpha-Tocopherol / Q000494:pharmacology
chemical_list: D015415:Biomarkers; D005752:Gastric Mucins; D010936:Plant Extracts; D024502:alpha-Tocopherol