title: Roux-en-Y gastric bypass increases systemic but not portal bile acid concentrations by decreasing hepatic bile acid uptake in minipigs.
abstract: Roux-en-Y gastric bypass (RYGB) surgery is widely used in the management of morbid obesity. RYGB improves metabolism independently of weight loss by still unknown mechanisms. Bile acids (BAs) are good candidates to explain this benefit, since they regulate metabolic homeostasis and their systemic concentrations increase upon RYGB. Here we analyzed the mechanisms underlying the increase in systemic BA concentrations after RYGB and the role of the liver therein. To this aim, we used the Göttingen-like minipig, a human-size mammalian model, which allows continuous sampling and simultaneous analysis of pre-hepatic portal and systemic venous blood. BA concentrations and pool composition were measured in portal blood, containing intestinal reabsorbed BAs and compared to systemic blood during a standardized meal test before and after RYGB. Systemic total BA concentrations increased after RYGB, due to an increase in conjugated BAs. Interestingly, the ratio of portal:systemic conjugated BAs decreased after RYGB, indicating a role for the liver in systemic BA concentrations changes. In line, hepatic expression of BA transporter genes decreased after RYGB. Our results show that the increase in systemic BAs after surgery is due to decreased selective hepatic recapture. Thus, alterations in hepatic function contribute to the increase in systemic BAs after RYGB.
authors.affiliation: Université de Lille, U1011 - EGID, Lille, France.|Inserm, U1011, Lille, France.|CHU Lille, Lille, France.|Institut Pasteur de Lille, Lille, France.|PECEM, Faculty of Medicine, UNAM, Mexico City, Mexico.
authors.forename: O
authors.initials: O
authors.lastname: Chávez-Talavera
authors.affiliation: CHU Lille, Lille, France.|Université de Lille, U1190-EGID, Lille, France.|Inserm, U1190, Lille, France.
authors.forename: G
authors.initials: G
authors.lastname: Baud
authors.affiliation: Université de Lille, U1011 - EGID, Lille, France.|Inserm, U1011, Lille, France.|CHU Lille, Lille, France.|Institut Pasteur de Lille, Lille, France.
authors.forename: V
authors.initials: V
authors.lastname: Spinelli
authors.affiliation: CHU Lille, Lille, France.|Université de Lille, U1190-EGID, Lille, France.|Inserm, U1190, Lille, France.
authors.forename: M
authors.initials: M
authors.lastname: Daoudi
authors.affiliation: Centre Universitaire de Mesures et d'Analyses, Université de Lille, Lille France.
authors.forename: M
authors.initials: M
authors.lastname: Kouach
authors.affiliation: Centre Universitaire de Mesures et d'Analyses, Université de Lille, Lille France.
authors.forename: J-F
authors.initials: JF
authors.lastname: Goossens
authors.affiliation: Université de Lille, U1011 - EGID, Lille, France.|Inserm, U1011, Lille, France.|CHU Lille, Lille, France.|Institut Pasteur de Lille, Lille, France.
authors.forename: E
authors.initials: E
authors.lastname: Vallez
authors.affiliation: CHU Lille, Lille, France.|Université de Lille, U1190-EGID, Lille, France.|Inserm, U1190, Lille, France.
authors.forename: R
authors.initials: R
authors.lastname: Caiazzo
authors.affiliation: CHU Lille, Lille, France.|Université de Lille, U1190-EGID, Lille, France.|Inserm, U1190, Lille, France.
authors.forename: M
authors.initials: M
authors.lastname: Ghunaim
authors.affiliation: CHU Lille, Lille, France.|Université de Lille, U1190-EGID, Lille, France.|Inserm, U1190, Lille, France.
authors.forename: T
authors.initials: T
authors.lastname: Hubert
authors.affiliation: Université de Lille, U1011 - EGID, Lille, France.|Inserm, U1011, Lille, France.|CHU Lille, Lille, France.|Institut Pasteur de Lille, Lille, France.
authors.forename: S
authors.initials: S
authors.lastname: Lestavel
authors.affiliation: Université de Lille, U1011 - EGID, Lille, France.|Inserm, U1011, Lille, France.|CHU Lille, Lille, France.|Institut Pasteur de Lille, Lille, France.
authors.forename: A
authors.initials: A
authors.lastname: Tailleux
authors.affiliation: Université de Lille, U1011 - EGID, Lille, France.|Inserm, U1011, Lille, France.|CHU Lille, Lille, France.|Institut Pasteur de Lille, Lille, France.
authors.forename: B
authors.initials: B
authors.lastname: Staels
authors.affiliation: CHU Lille, Lille, France.|Université de Lille, U1190-EGID, Lille, France.|Inserm, U1190, Lille, France.
authors.forename: F
authors.initials: F
authors.lastname: Pattou
mesh_terms: D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism*; D004195:Disease Models, Animal; D015390:Gastric Bypass; D008099:Liver / Q000378:metabolism*; D008297:Male; D009767:Obesity, Morbid / Q000378:metabolism* / Q000601:surgery*; D013552:Swine; D013556:Swine, Miniature / Q000378:metabolism*; D015431:Weight Loss / Q000502:physiology
chemical_list: D001647:Bile Acids and Salts