title: A Disease Model of Muscle Necrosis Caused byAeromonas dhakensisInfection inCaenorhabditis elegans.
abstract: A variety of bacterial infections cause muscle necrosis in humans.Caenorhabditis eleganshas epidermis and bands of muscle that resemble soft-tissue structures in mammals and humans. Here, we developed a muscle necrosis model caused byAeromonas dhakensisinfection inC. elegans. Our data showed thatA. dhakensisinfected and killedC. elegansrapidly. Characteristic muscle damage inC. elegansinduced byA. dhakensiswas demonstratedin vivo. Relative expression levels of host necrosis-associated genes,asp-3,asp-4, andcrt-1increased significantly afterA. dhakensisinfection. The RNAi sensitive NL2099rrf-3 (pk1426) worms with knockdown of necrosis genes ofcrt-1andasp-4by RNAi showed prolonged survival afterA. dhakensisinfection. Specifically knockdown ofcrt-1andasp-4by RNAi in WM118 worms, which restricted RNAi only to the muscle cells, conferred significant resistance toA. dhakensisinfection. In contrast, the severity of muscle damage and toxicity produced by theA. dhakensishemolysin-deletion mutant is attenuated. In another example, shiga-like toxin-producing enterohemorrhagicE. coli(EHEC) known to elicit toxicity toC. eleganswith concomitant enteropathogenicty, did not cause muscle necrosis asA. dhakensisdid. Taken together, these results show thatAeromonasinfection induces muscle necrosis and rapid death of infectedC. elegans, which are similar to muscle necrosis in humans, and then validate the value of theC. elegansmodel withA. dhakensisinfection in studyingAeromonaspathogenicity.
authors.affiliation: Institute of Clinical Medicine, College of Medicine, National Cheng Kung UniversityTainan, Taiwan; Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung UniversityTainan, Taiwan; Department of Internal Medicine, College of Medicine, National Cheng Kung UniversityTainan, Taiwan.
authors.forename: Po-Lin
authors.initials: PL
authors.lastname: Chen
authors.affiliation: Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University Tainan, Taiwan.
authors.forename: Yi-Wei
authors.initials: YW
authors.lastname: Chen
authors.affiliation: Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University Tainan, Taiwan.
authors.forename: Chun-Chun
authors.initials: CC
authors.lastname: Ou
authors.affiliation: Institute of Oral Medicine, College of Medicine, National Cheng Kung University Tainan, Taiwan.
authors.forename: Tzer-Min
authors.initials: TM
authors.lastname: Lee
authors.affiliation: Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung UniversityTainan, Taiwan; National Institute of Infectious Diseases and Vaccinology, National Health Research InstitutesTainan, Taiwan.
authors.forename: Chi-Jung
authors.initials: CJ
authors.lastname: Wu
authors.affiliation: Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung UniversityTainan, Taiwan; Department of Internal Medicine, College of Medicine, National Cheng Kung UniversityTainan, Taiwan.
authors.forename: Wen-Chien
authors.initials: WC
authors.lastname: Ko
authors.affiliation: Department of Internal Medicine, College of Medicine, National Cheng Kung UniversityTainan, Taiwan; Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung UniversityTainan, Taiwan.
authors.forename: Chang-Shi
authors.initials: CS
authors.lastname: Chen
keywords: Aeromonas dhakensis; Caenorhabditis elegans; disease model; infection; muscle necrosis