title: A novel chimeric Newcastle disease virus vectored vaccine against highly pathogenic avian influenza virus.
abstract: Avian influenza (AI) is an economically-important disease of poultry worldwide. The use of vaccines to control AI has increased because of frequent outbreaks of the disease in endemic countries. Newcastle disease virus (NDV) vectored vaccine has shown to be effective in protecting chickens against a highly pathogenic avian influenza virus (HPAIV) infection. However, preexisting antibodies to NDV vector might affect protective efficacy of the vaccine in the field. As an alternative strategy, we evaluated vaccine efficacy of a chimeric NDV vectored vaccine in which the ectodomains of F and HN proteins were replaced by those of avian paramyxovirus serotype-2. The chimeric NDV vector stably expressed the HA protein in vivo, did not cross-react with NDV, was attenuated to be used as a safe vaccine, and provided a partial protection of 1-day-old immunized chickens against HPAIV subtype H5N1challenge, indicating its potential use for early protection of chickens.
authors.affiliation: Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, MD, USA.
authors.forename: Shin-Hee
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, MD, USA.
authors.forename: Anandan
authors.initials: A
authors.lastname: Paldurai
authors.affiliation: Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, MD, USA. Electronic address: ssamal@umd.edu.
authors.forename: Siba K
authors.initials: SK
authors.lastname: Samal
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D018109:Avulavirus / Q000235:genetics / Q000276:immunology; D002642:Chick Embryo; D002645:Chickens; D003429:Cross Reactions; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics* / Q000276:immunology*; D007114:Immunization; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines; D005585:Influenza in Birds / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D009521:Newcastle Disease / Q000821:virology; D009522:Newcastle disease virus / Q000235:genetics* / Q000276:immunology*; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology
chemical_list: D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines
keywords: Avian influenza virus; Chickens; Newcastle disease virus; Protection; Vaccine