title: Electrochemiluminescent chemodosimeter based on iridium(III) complex for point-of-care detection of homocysteine levels.
abstract: Elevated levels of plasma homocysteine (Hcy) are an independent risk factor for cardiovascular disease. Although a routine, rapid, and simple determination of Hcy levels is highly desired, the existing methods are practically limited because of complicated sample preparation and bulky instrumentation. Herein, we report a chemodosimetric approach for one-step analysis of Hcy levels based on the electrochemiluminescence (ECL). A rationally designed cyclometalated iridium(III) complex possessing a phenylisoquinoline main ligand underwent a selective ring-formation reaction with Hcy to generate a binding adduct, which enabled producing highly luminescent excited states, and yielded strong ECL signals on the surface of electrode without any use of enzymes or antibodies. The level of Hcy was successfully monitored by the ECL increment with a linear correlation between 0 and 40µM in 99.9% aqueous media. The approach required neither sample preparation nor bulky instrument, suggesting the point-of-care testing of Hcy levels, and is potentially useful for routine, cost-effective, and precautionary diagnosis of various cardiovascular diseases.
authors.affiliation: Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151-747, Republic of Korea.
authors.forename: Hoon Jun
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151-747, Republic of Korea.
authors.forename: Kyung-Sik
authors.initials: KS
authors.lastname: Lee
authors.affiliation: Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151-747, Republic of Korea.
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Jeon
authors.affiliation: Department of Chemistry, College of Natural Science, Soongsil University, Seoul 156-743, Republic of Korea. Electronic address: extant@ssu.ac.kr.
authors.forename: Ik-Soo
authors.initials: IS
authors.lastname: Shin
authors.affiliation: Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151-747, Republic of Korea. Electronic address: jihong@snu.ac.kr.
authors.forename: Jong-In
authors.initials: JI
authors.lastname: Hong
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation; D056831:Coordination Complexes / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000295:instrumentation; D006710:Homocysteine / Q000032:analysis / Q000097:blood*; D006801:Humans; D007495:Iridium / Q000737:chemistry*; D007546:Isoquinolines / Q000737:chemistry*; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements / Q000295:instrumentation*; D008958:Models, Molecular; D019095:Point-of-Care Systems
chemical_list: D056831:Coordination Complexes; D007546:Isoquinolines; D049408:Luminescent Agents; D006710:Homocysteine; D007495:Iridium
keywords: Chemodosimeter; Electrochemiluminescence (ECL); Homocysteine (Hcy); Iridium complex