title: Beta-adrenergic antagonist propranolol inhibits mammalian cell lysosome spreading and invasion by Trypanosoma cruzi metacyclic forms.
abstract: The involvement of β-adrenergic receptor (β-AR) in host cell invasion by Trypanosoma cruzi metacyclic trypomastigote (MT) is not known. We examined whether isoproterenol, an agonist of β-AR, or nonselective β-blocker propranolol affected MT internalization mediated the stage-specific surface molecule gp82. Treatment of HeLa cells with propranolol significantly inhibited MT invasion whereas isoproterenol had no effect. Propranolol, but not isoproterenol, also inhibited the lysosome spreading required for gp82-dependent MT invasion. The effect of propranolol in inhibiting MT internalization was not due to the prevention of gp82 interaction with β-AR. It was mainly associated with its ability to impair lysosome spreading.
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, R. Pedro de Toledo, 669 - 6(o) andar, 04039-032, São Paulo, SP, Brazil.
authors.forename: Silene
authors.initials: S
authors.lastname: Macedo
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, R. Pedro de Toledo, 669 - 6(o) andar, 04039-032, São Paulo, SP, Brazil.
authors.forename: João Paulo Ferreira
authors.initials: JPF
authors.lastname: Rodrigues
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, R. Pedro de Toledo, 669 - 6(o) andar, 04039-032, São Paulo, SP, Brazil.
authors.forename: Sergio
authors.initials: S
authors.lastname: Schenkman
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, R. Pedro de Toledo, 669 - 6(o) andar, 04039-032, São Paulo, SP, Brazil. Electronic address: nyoshida@unifesp.br.
authors.forename: Nobuko
authors.initials: N
authors.lastname: Yoshida
mesh_terms: D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology*; D000977:Antiparasitic Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D014355:Chagas Disease / Q000469:parasitology / Q000473:pathology; D006367:HeLa Cells; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology*; D008247:Lysosomes / Q000378:metabolism*; D011433:Propranolol / Q000494:pharmacology*; D015800:Protozoan Proteins / Q000378:metabolism; D011943:Receptors, Adrenergic, beta / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000254:growth & development* / Q000378:metabolism; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000378:metabolism
chemical_list: D000318:Adrenergic beta-Agonists; D000319:Adrenergic beta-Antagonists; D000977:Antiparasitic Agents; D015800:Protozoan Proteins; D011943:Receptors, Adrenergic, beta; D014643:Variant Surface Glycoproteins, Trypanosoma; C527132:glycoprotein GP82, Trypanosoma cruzi; D011433:Propranolol; D007545:Isoproterenol
keywords: Host cell invasion; Metacyclic trypomastigote; Propranolol; Trypanosoma cruzi