title: S100A8 protein attenuates airway hyperresponsiveness by suppressing the contraction of airway smooth muscle.
abstract: Airway hyperresponsiveness (AHR) is a major clinical problem in allergic asthma mainly caused by the hypercontractility of airway smooth muscles (ASM). S100A8 is an important member of the S100 calcium-binding protein family with a potential to regulate cell contractility. Here, we analyze the potential of S100A8 to regulate allergen-induced AHR and ASM contraction. Treatment with recombinant S100A8 (rS100A8) diminished airway hyperresponsiveness in OVA-sensitized rats. ASM contraction assays showed that rS100A8 reduced hypercontractility in both isolated tracheal rings and primary ASM cells treated by acetylcholine. rS100A8 markedly rescued the phosphorylation level of myosin light chain induced by acetylcholine in ASM cells. These results show that rS100A8 plays a protective role in regulating AHR in asthma by inhibiting ASM contraction. These results support S100A8 as a novel therapeutic target to control ASM contraction in asthma.
authors.affiliation: Shanghai Research Institute of Acupuncture and Meridian, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Yu-Dong
authors.initials: YD
authors.lastname: Xu
authors.affiliation: Shanghai Research Institute of Acupuncture and Meridian, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Shanghai Research Institute of Acupuncture and Meridian, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Lei-Miao
authors.initials: LM
authors.lastname: Yin
authors.affiliation: Shanghai Research Institute of Acupuncture and Meridian, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Gyoung-Hee
authors.initials: GH
authors.lastname: Park
authors.affiliation: Shanghai Research Institute of Acupuncture and Meridian, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China; Center of Immunology and Inflammation, Rutgers-New Jersey Medical School, Rutgers University, Newark, NJ 07101, USA. Electronic address: Luis.Ulloa@Rutgers.edu.
authors.forename: Luis
authors.initials: L
authors.lastname: Ulloa
authors.affiliation: Shanghai Research Institute of Acupuncture and Meridian, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China. Electronic address: yyq@shutcm.edu.cn.
authors.forename: Yong-Qing
authors.initials: YQ
authors.lastname: Yang
mesh_terms: D000109:Acetylcholine / Q000008:administration & dosage; D000818:Animals; D040501:Calgranulin A / Q000502:physiology*; D002478:Cells, Cultured; D009119:Muscle Contraction / Q000502:physiology; D009130:Muscle, Smooth / Q000502:physiology*; D018994:Myosin Light Chains / Q000378:metabolism; D010047:Ovalbumin / Q000008:administration & dosage; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology; D012130:Respiratory Hypersensitivity / Q000517:prevention & control*
chemical_list: D040501:Calgranulin A; D018994:Myosin Light Chains; D011994:Recombinant Proteins; D010047:Ovalbumin; D000109:Acetylcholine
keywords: Airway hyperresponsiveness; Airway smooth muscle; Asthma; Contraction; S100A8