title: MiR-338-5p Promotes Inflammatory Response of Fibroblast-Like Synoviocytes in Rheumatoid Arthritis via Targeting SPRY1.
abstract: Our purpose is to study the roles of microRNA-338-5p (miR-338-5p) on the proliferation, invasion, and inflammatory response of fibroblast-like synoviocytes (SFs) in rheumatoid arthritis patients by regulating SPRY1. The target relationship between miR-338-5p and SPRY1 was validated through luciferase reporter system. The expression of miR-338-5p and SPRY1 in synovial tissues and synovial cells were detected using RT-PCR and western blot. The mimics and inhibitors of miR-338-5p were transfected into SFs. MTT, Transwell, and ELISA assays were used to analyze cell proliferation, invasiveness, and the secreted extracellular pro-inflammatory cytokines (such as IL-1a, IL-6, COX2) levels of SFs. MiR-338-5p was highly expressed in rheumatoid arthritis tissues and cells, and directly down-regulated the expression of SPRY1 in the SFs of rheumatoid arthritis patients. Cell proliferation, invasiveness and the expression level of pro-inflammatory cytokines in synovial cells increased after the transfection of miR-338-5p mimics, while the proliferation, invasion and expression level of pro-inflammatory cytokines decreased after the transfection of miR-338-5p inhibitors. In conclusion,miR-338-5p promoted the proliferation, invasion and inflammatory reaction in SFs of rheumatoid arthritis by directly down-regulating SPRY1 expression. J. Cell. Biochem. 118: 2295-2301, 2017. © 2017 Wiley Periodicals, Inc.
authors.affiliation: Department of Sports Medicine and Joint Surgery/Orthopaedics, First Affiliated Hospital of China Medical University, Shenyang, Liaoning, 110001, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Sports Medicine and Joint Surgery/Orthopaedics, First Affiliated Hospital of China Medical University, Shenyang, Liaoning, 110001, China.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Sports Medicine and Joint Surgery/Orthopaedics, First Affiliated Hospital of China Medical University, Shenyang, Liaoning, 110001, China.
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Liang
authors.affiliation: Department of Sports Medicine and Joint Surgery/Orthopaedics, First Affiliated Hospital of China Medical University, Shenyang, Liaoning, 110001, China.
authors.forename: Lutian
authors.initials: L
authors.lastname: Yao
authors.affiliation: Department of Sports Medicine and Joint Surgery/Orthopaedics, First Affiliated Hospital of China Medical University, Shenyang, Liaoning, 110001, China.
authors.forename: Shizhong
authors.initials: S
authors.lastname: Gu
authors.affiliation: Department of Sports Medicine/Joint Surgery, The People's Hospital of China Medical University, Shenyang, Liaoning, 110001, China.
authors.forename: Xizhuang
authors.initials: X
authors.lastname: Bai
mesh_terms: D000818:Animals; D001172:Arthritis, Rheumatoid / Q000139:chemically induced / Q000276:immunology* / Q000378:metabolism*; D015153:Blotting, Western; D049109:Cell Proliferation / Q000235:genetics / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen / Q000633:toxicity; D051546:Cyclooxygenase 2 / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D053582:Interleukin-1alpha / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013583:Synovial Membrane / Q000166:cytology / Q000378:metabolism; D000070918:Synoviocytes / Q000166:cytology* / Q000378:metabolism*
chemical_list: D053582:Interleukin-1alpha; D015850:Interleukin-6; C532050:MIRN338 microRNA, human; D035683:MicroRNAs; D003094:Collagen; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human
keywords: FIBROBLAST-LIKE SYNOVIAL CELLS (SFs); MIR-338-5p; RHEUMATOID ARTHRITIS; SPRY1