title: Single-Cell Genomics: Approaches and Utility in Immunology.
abstract: Single-cell genomics offers powerful tools for studying immune cells, which make it possible to observe rare and intermediate cell states that cannot be resolved at the population level. Advances in computer science and single-cell sequencing technology have created a data-driven revolution in immunology. The challenge for immunologists is to harness computing and turn an avalanche of quantitative data into meaningful discovery of immunological principles, predictive models, and strategies for therapeutics. Here, we review the current literature on computational analysis of single-cell RNA-sequencing data and discuss underlying assumptions, methods, and applications in immunology, and highlight important directions for future research.
authors.affiliation: Committee on Immunology, University of Chicago, Chicago, IL 60637, USA.
authors.forename: Karlynn E
authors.initials: KE
authors.lastname: Neu
authors.affiliation: Toyota Technological Institute at Chicago, Chicago, IL 60637, USA.
authors.forename: Qingming
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Committee on Immunology, University of Chicago, Chicago, IL 60637, USA.
authors.forename: Patrick C
authors.initials: PC
authors.lastname: Wilson
authors.affiliation: Toyota Technological Institute at Chicago, Chicago, IL 60637, USA. Electronic address: aakhan@uchicago.edu.
authors.forename: Aly A
authors.initials: AA
authors.lastname: Khan
mesh_terms: D000486:Allergy and Immunology / Q000639:trends*; D000818:Animals; D019295:Computational Biology; D023281:Genomics; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D007107:Immune System; D007158:Immunologic Techniques; D007167:Immunotherapy / Q000379:methods / Q000639:trends*; D059010:Single-Cell Analysis
keywords: dimensionality reduction; immune repertoire; single-cell RNA-sequencing; visualization