title: SH2 Domain Histochemistry.
abstract: Among posttranslational modifications, the phosphorylation of tyrosine residues is a key modification in cell signaling. Because of its biological importance, characterization of the cellular state of tyrosine phosphorylation is of great interest. Based on the unique properties of endogenously expressed SH2 domains recognizing tyrosine phosphorylated signaling proteins with high specificity we have developed an alternative approach, coined SH2 profiling, enabling us to decipher complex patterns of tyrosine phosphorylation in various normal and cancerous tissues. So far, SH2 profiling has largely been applied for the analysis of protein extracts with the limitation that information on spatial distribution and intensity of tyrosine phosphorylation within a tissue is lost. Here, we describe a novel SH2 domain based strategy for differential characterization of the state of tyrosine phosphorylation in formaldehyde-fixed and paraffin-embedded tissues. This approach demonstrates that SH2 domains may serve as very valuable tools for the analysis of the differential state of tyrosine phosphorylation in primary tissues fixed and processed under conditions frequently applied by routine pathology laboratories.
authors.affiliation: Research Institute Children's Cancer Center and Department of Pediatric Hematology and Oncology, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, Building N63, 2nd Floor, 20251, Hamburg, Germany.
authors.forename: Sophia
authors.initials: S
authors.lastname: Buhs
authors.affiliation: Research Institute Children's Cancer Center and Department of Pediatric Hematology and Oncology, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, Building N63, 2nd Floor, 20251, Hamburg, Germany. nollau@uke.de.
authors.forename: Peter
authors.initials: P
authors.lastname: Nollau
mesh_terms: D007150:Immunohistochemistry / Q000379:methods*; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism; D011485:Protein Binding; D015398:Signal Transduction; D014443:Tyrosine / Q000378:metabolism; D018909:src Homology Domains
chemical_list: D019000:Phosphotyrosine; D014443:Tyrosine
keywords: Cancer; Histochemistry; Phosphotyrosine; SH2 domain; Signal amplification; Signal transduction; Src-homology 2; Tyrosine phosphorylation