title: MicroRNA-21 Inhibits the Apoptosis of Osteosarcoma Cell Line SAOS-2 via Targeting Caspase 8.
abstract: Currently, multiple microRNAs (miRNAs) have been found to play vital roles in the pathogenesis of osteosarcoma. This study aimed to investigate the role of miR-21 in osteosarcoma. The level of miR-21 in 20 pairs of osteosarcoma and corresponding adjacent tissues was monitored by qPCR. Human osteosarcoma cell line SAOS-2 was transfected with either miR-21 mimic or miR-21 inhibitor, and then cell viability, survival, and apoptosis were measured by MTT, colony formation assay, and flow cytometry. A target of miR-21 was predicted by the microRNA.org database and verified in vitro by using luciferase reporter, qPCR, and Western blot analyses. Finally, cells were cotransfected with siRNA against caspase 8 and miR-21 inhibitor, and the apoptotic cell rate was determined again. Results showed that the mRNA level of miR-21 was highly expressed in osteosarcoma tissues compared with adjacent tissues. Overexpression of miR-21 improved cell viability and survival but suppressed apoptosis. Caspase 8 was a direct target of miR-21, and it was negatively regulated by miR-21. Moreover, miR-21 suppression attenuated caspase 8 silencing and induced the decrease in apoptosis. In conclusion, overexpression of miR-21 suppressed SAOS-2 cell apoptosis via directly targeting caspase 8.
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.forename: Hehuan
authors.initials: H
authors.lastname: Xia
authors.forename: Junming
authors.initials: J
authors.lastname: Cao
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Wang
authors.forename: Yipeng
authors.initials: Y
authors.lastname: Yang
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Lin
mesh_terms: D000328:Adult; D017209:Apoptosis / Q000235:genetics*; D001859:Bone Neoplasms / Q000235:genetics*; D053181:Caspase 8 / Q000235:genetics*; D045744:Cell Line, Tumor; D002470:Cell Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D012516:Osteosarcoma / Q000235:genetics*; D034622:RNA Interference; D055815:Young Adult
chemical_list: C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D053181:Caspase 8