title: Dual-focus coherent anti-Stokes Raman scattering microscopy using a compact two-beam fiber laser source.
abstract: We have developed a dual-focus coherent anti-Stokes Raman scattering (CARS) microscope based on a dual output, compact fiber laser source. The underlying concepts of time-multiplexed, two-beam scanning and demultiplexed detection that we already employed for second-harmonic generation are here naturally extended for CARS microscopy. The layout of a robust, all-fiber laser source was reconfigured to provide two outputs, each containing the two colors necessary for the CARS process. The utilization of the design for simultaneously imaging two laterally or axially separated fields of view and, thus, inherently speeding up the image acquisition process, is demonstrated on human artery tissue samples.
authors.forename: Fisseha Bekele
authors.initials: FB
authors.lastname: Legesse
authors.forename: Tobias
authors.initials: T
authors.lastname: Meyer
authors.forename: Sandro
authors.initials: S
authors.lastname: Heuke
authors.forename: Thomas
authors.initials: T
authors.lastname: Gottschall
authors.forename: Torbjörn
authors.initials: T
authors.lastname: Pascher
authors.forename: Jens
authors.initials: J
authors.lastname: Limpert
authors.forename: Andreas
authors.initials: A
authors.lastname: Tünnermann
authors.forename: Michael
authors.initials: M
authors.lastname: Schmitt
authors.forename: Jürgen
authors.initials: J
authors.lastname: Popp
mesh_terms: D001158:Arteries / Q000502:physiology*; D006801:Humans; D007834:Lasers; D013059:Spectrum Analysis, Raman / Q000379:methods*