title: MicroRNA-150 targets ELK1 and modulates the apoptosis induced by ox-LDL in endothelial cells.
abstract: Atherosclerosis, a chronic inflammatory disease, is the major cause of life-threatening complications such as myocardial infarction and stroke. Endothelial cells (ECs) apoptosis plays a vital role in the initiation and progression of atherosclerosis. Although a subset of microRNAs (miRNAs) have been identified as critical regulators of atherosclerosis, studies on their participation in endothelial apoptosis in atherosclerosis have been limited. In the current study, we show that miRNA-150 (miR-150) expression was substantially up-regulated during the oxidized low-density lipoprotein (ox-LDL)-induced apoptosis in human umbilical cord vein endothelial cells (HUVECs). Forced expression of miR-150 enhanced apoptosis in ECs, whereas inhibition of miR-150 could partly alleviate apoptotic cell death mediated by ox-LDL. Further analysis identified ELK1 as a direct target of miR-150, and ELK1 knockdown abolished the anti-apoptotic effect of miR-150 inhibitor. These findings reveal a novel role of miR-150 in endothelial apoptosis and indicate a therapeutic potential of miR-150 for endothelial dysfunction and atherosclerosis.
authors.affiliation: Department of Neurology, The Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe Road, Guangzhou, 510630, Guangdong, People's Republic of China.
authors.forename: Bing
authors.initials: B
authors.lastname: Qin
authors.affiliation: Department of Neurology, The Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe Road, Guangzhou, 510630, Guangdong, People's Republic of China.
authors.forename: Yaqing
authors.initials: Y
authors.lastname: Shu
authors.affiliation: Department of Neurology, The Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe Road, Guangzhou, 510630, Guangdong, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Department of Neurology, The Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe Road, Guangzhou, 510630, Guangdong, People's Republic of China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Lu
authors.affiliation: Department of Neurology, The Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe Road, Guangzhou, 510630, Guangdong, People's Republic of China.
authors.forename: Yinyao
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, People's Republic of China.
authors.forename: Huan
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Neurology, The Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe Road, Guangzhou, 510630, Guangdong, People's Republic of China. luzhengqi1828@126.com.
authors.forename: Zhengqi
authors.identifier: http://orcid.org/0000-0002-8889-7697
authors.initials: Z
authors.lastname: Lu
mesh_terms: D020413:3' Untranslated Regions; D017209:Apoptosis; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects; D005786:Gene Expression Regulation; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D035683:MicroRNAs / Q000235:genetics*; D050807:ets-Domain Protein Elk-1 / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D008077:Lipoproteins, LDL; D035683:MicroRNAs; D050807:ets-Domain Protein Elk-1; C494112:ELK1 protein, human; C533097:MIR150, human; C099252:oxidized low density lipoprotein
keywords: Apoptosis; Atherosclerosis; ELK1; HUVECs; MicroRNA-150