title: Isogarcinol Extracted from Garcinia mangostana L. Ameliorates Imiquimod-Induced Psoriasis-like Skin Lesions in Mice.
abstract: Isogarcinol (YDIS), a natural compound extracted from Garcinia mangostana L., has a significant immunosuppressive effect on systemic lupus erythematosus and rheumatoid arthritis. This paper reports that it reduced imiquimod-induced psoriasis-like skin lesions in mice. It strongly attenuated the aberrant proliferation and differentiation of keratinocytes. Moreover, the expression of genes involving the interleukin-23 (IL-23)/T-helper 17 (Th17) axis was significantly inhibited in the dorsal skin of the YDIS-treated mice, as was that of the other pro-inflammatory factors TNF-α, IL-2, and even interferon (IFN)-γ. Furthermore, YDIS prevented the abnormal distribution of T cell types and suppressed the differentiation of CD4+T cells into Th17 cells in the spleens of mice exposed to imiquimod. Interestingly, it elevated numbers of regulatory T cells (Tregs) in the spleen and boosted IL-10 expression in the skin. In agreement with the above, YDIS increased serum IL-10 and reduced serum IL-17. It also caused less damage to the liver and, especially, kidneys of mice than cyclosporine A (CsA). In vitro, YDIS caused more death of HaCaT keratinocytes than CsA. It also strongly inhibited inflammatory factor expression in lipopolysaccharide (LPS)-stimulated HaCaT cells. These findings suggest that YDIS is a promising immunosuppressive agent for treating psoriasis.
authors.affiliation: Department of Biochemistry and Molecular Biology, Gene Engineering and Biotechnology Beijing Key Laboratory, College of Life Sciences, Beijing Normal University , Beijing 100875, People's Republic of China.
authors.forename: Shanzao
authors.initials: S
authors.lastname: Chen
authors.affiliation: Haikou Qili Pharmaceutical Company, Ltd. , Haikou 570216, People's Republic of China.
authors.forename: Kesheng
authors.initials: K
authors.lastname: Han
authors.affiliation: Department of Biochemistry and Molecular Biology, Gene Engineering and Biotechnology Beijing Key Laboratory, College of Life Sciences, Beijing Normal University , Beijing 100875, People's Republic of China.
authors.forename: Hu
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, Gene Engineering and Biotechnology Beijing Key Laboratory, College of Life Sciences, Beijing Normal University , Beijing 100875, People's Republic of China.
authors.forename: Juren
authors.initials: J
authors.lastname: Cen
authors.affiliation: College of Pharmacy, Hubei University of Chinese Medicine , Wuhan 430061, People's Republic of China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Pharmacy, Hubei University of Chinese Medicine , Wuhan 430061, People's Republic of China.
authors.forename: Hezhen
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Biochemistry and Molecular Biology, Gene Engineering and Biotechnology Beijing Key Laboratory, College of Life Sciences, Beijing Normal University , Beijing 100875, People's Republic of China.
authors.forename: Qun
authors.identifier: http://orcid.org/0000-0001-6241-0679
authors.initials: Q
authors.lastname: Wei
mesh_terms: D000634:Aminoquinolines / Q000008:administration & dosage*; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D029762:Garcinia mangostana / Q000737:chemistry*; D006801:Humans; D000077271:Imiquimod; D007376:Interleukin-2 / Q000235:genetics / Q000276:immunology; D053759:Interleukin-23 / Q000235:genetics / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D010936:Plant Extracts / Q000008:administration & dosage*; D011565:Psoriasis / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology; D012867:Skin / Q000187:drug effects / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology; D058504:Th17 Cells / Q000187:drug effects / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha
chemical_list: D000634:Aminoquinolines; D007376:Interleukin-2; D053759:Interleukin-23; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D000077271:Imiquimod
keywords: HaCaT; T-helper 17 cell; cyclosporine A; imiquimod-induced; isogarcinol; psoriasis; regulatory T cell