title: Effect of resveratrol on platelet aggregation by fibrinogen protection.
abstract: The effect of resveratrol (RSV) in inhibiting platelet adhesion and aggregation, as well as fibrinogen (FBG) conformational changes promoted by epinephrine (EP), were studied, by using complementary experimental techniques. NMR and IR spectroscopies were used to investigate possible protective effects by RSV towards FBG, in presence of EP. The protective effect of RSV towards FBG was highlighted by spin nuclear relaxation experiments that were interpreted for determining the thermodynamic equilibrium constants of FBG-EP interaction, and by infrared measurements, that showed EP-induced conformational changes of FBG. The ability of RSV in inhibiting platelet adhesion and aggregation promoted by EP was evaluated by scanning electron microscopy (SEM), measuring the platelet adhesion and aggregation degree, in comparison to data obtained for platelet aggregation in platelet rich plasma (PRP). The experimental combined approach pointed out that RSV is able to protect both FBG and platelets from the denaturant and aggregating action of EP at stress level concentration.
authors.affiliation: Department of Biotechnologies, Chemistry, and Pharmacy, Via Aldo Moro 2, 53100 Siena, Italy; CSGI-Research Center for Colloids and Nanoscience, Via della Lastruccia 3, 50019 Sesto Fiorentino, FI, Italy. Electronic address: claudia.bonechi@unisi.it.
authors.forename: Claudia
authors.initials: C
authors.lastname: Bonechi
authors.affiliation: Department of Biotechnologies, Chemistry, and Pharmacy, Via Aldo Moro 2, 53100 Siena, Italy; INSTM-National Interuniversity Consortium of Materials Science and Technology, Via G. Giusti 9, 50121 Firenze, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Lamponi
authors.affiliation: Department of Biotechnologies, Chemistry, and Pharmacy, Via Aldo Moro 2, 53100 Siena, Italy; CSGI-Research Center for Colloids and Nanoscience, Via della Lastruccia 3, 50019 Sesto Fiorentino, FI, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Donati
authors.affiliation: Department of Biotechnologies, Chemistry, and Pharmacy, Via Aldo Moro 2, 53100 Siena, Italy; CSGI-Research Center for Colloids and Nanoscience, Via della Lastruccia 3, 50019 Sesto Fiorentino, FI, Italy.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Tamasi
authors.affiliation: Department of Biotechnologies, Chemistry, and Pharmacy, Via Aldo Moro 2, 53100 Siena, Italy; INSTM-National Interuniversity Consortium of Materials Science and Technology, Via G. Giusti 9, 50121 Firenze, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Consumi
authors.affiliation: Department of Biotechnologies, Chemistry, and Pharmacy, Via Aldo Moro 2, 53100 Siena, Italy; INSTM-National Interuniversity Consortium of Materials Science and Technology, Via G. Giusti 9, 50121 Firenze, Italy.
authors.forename: Gemma
authors.initials: G
authors.lastname: Leone
authors.affiliation: Department of Biotechnologies, Chemistry, and Pharmacy, Via Aldo Moro 2, 53100 Siena, Italy; CSGI-Research Center for Colloids and Nanoscience, Via della Lastruccia 3, 50019 Sesto Fiorentino, FI, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Rossi
authors.affiliation: Department of Biotechnologies, Chemistry, and Pharmacy, Via Aldo Moro 2, 53100 Siena, Italy; INSTM-National Interuniversity Consortium of Materials Science and Technology, Via G. Giusti 9, 50121 Firenze, Italy.
authors.forename: Agnese
authors.initials: A
authors.lastname: Magnani
mesh_terms: D004837:Epinephrine / Q000494:pharmacology; D005340:Fibrinogen / Q000737:chemistry* / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D064347:Microscopy, Electrochemical, Scanning; D010974:Platelet Aggregation / Q000187:drug effects*; D020011:Protective Agents / Q000494:pharmacology; D011487:Protein Conformation / Q000187:drug effects; D000077185:Resveratrol; D013057:Spectrum Analysis; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D020011:Protective Agents; D013267:Stilbenes; D005340:Fibrinogen; D000077185:Resveratrol; D004837:Epinephrine
keywords: FTIR; Fibrinogen; NMR; Platelet adhesion and aggregation; Resveratrol