title: Determination of hexitols by reversed phase liquid chromatography using on-line complexation with molybdate ion.
abstract: A new approach is proposed to determine three hexitols (mannitol, sorbitol and dulcitol) by reversed phase liquid chromatography and ultraviolet detection. When molybdate ion is added to the mobile phase, it forms complexes with hexitols that can be separated on a reversed phase C30 column and detected by UV absorption at 247 nm. The mobile phase (pH 3.1) consisted of 0.1 mM disodium molybdate and 1 mM phosphoric acid. Other sugar alcohols, such as erythritol and xylitol, and glucose could not be detected under these conditions. The quantification limits of the examined three hexitols calculated at S/N = 10 were 0.001 mM and the detector response was linear in the range 0.001-0.3 mM. The method successfully measured these hexitols in candy samples, and the results obtained by the proposed method agreed well with those obtained by enzymatic methods.
authors.affiliation: Toyama Institute of Health, 17-1 Nakataikoyama, Imizu 939-0363, Japan. Electronic address: tomoko.kenmei@pref.toyama.lg.jp.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kemmei
authors.affiliation: Department of Chemistry, Faculty of Science, Tokai University, 4-1-1 Kitakaname, Hiratsuka 259-1292, Japan.
authors.forename: Shuji
authors.initials: S
authors.lastname: Kodama
authors.affiliation: Department of Biological Chemistry, College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai 487-8501, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yamamoto
authors.affiliation: Department of Biological Chemistry, College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai 487-8501, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Inoue
authors.affiliation: Institute of Nature and Environmental Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Kazuichi
authors.initials: K
authors.lastname: Hayakawa
mesh_terms: D002182:Candy / Q000032:analysis*; D056148:Chromatography, Reverse-Phase; D008982:Molybdenum; D013402:Sugar Alcohols / Q000032:analysis*
chemical_list: D013402:Sugar Alcohols; C543192:hexitol; C044659:molybdate; D008982:Molybdenum
keywords: Hexitols; Molybdate ion; Reversed phase liquid chromatography; Sugar alcohols; Ultraviolet detection