title: Virome of US bovine calf serum.
abstract: Using viral metagenomics we analyzed four bovine serum pools assembled from 715 calves in the United States. Two parvoviruses, bovine parvovirus 2 (BPV2) and a previously uncharacterized parvovirus designated as bosavirus (BosaV), were detected in 3 and 4 pools respectively and their complete coding sequences generated. Based on NS1 protein identity, bosavirus qualifies as a member of a new species in the copiparvovirus genus. Also detected were low number of reads matching ungulate tetraparvovirus 2, bovine hepacivirus, and several papillomaviruses. This study further characterizes the diversity of viruses in calf serum with the potential to infect fetuses and through fetal bovine serum contaminate cell cultures.
authors.affiliation: Blood Systems Research Institute, San Francisco, CA, USA; Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA, USA; Department of Virology, University of Helsinki, Finland.
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Sadeghi
authors.affiliation: Blood Systems Research Institute, San Francisco, CA, USA; Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA, USA.
authors.forename: Beatrix
authors.initials: B
authors.lastname: Kapusinszky
authors.affiliation: Department of Biomedical Sciences and Pathobiology, College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
authors.forename: Danielle M
authors.initials: DM
authors.lastname: Yugo
authors.affiliation: Blood Systems Research Institute, San Francisco, CA, USA; Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA, USA.
authors.forename: Tung Gia
authors.initials: TG
authors.lastname: Phan
authors.affiliation: Blood Systems Research Institute, San Francisco, CA, USA; Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA, USA.
authors.forename: Xutao
authors.initials: X
authors.lastname: Deng
authors.affiliation: Department of Dairy Science, College of Agriculture and Life Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
authors.forename: Isis
authors.initials: I
authors.lastname: Kanevsky
authors.affiliation: The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, Scotland, UK.
authors.forename: Tanja
authors.initials: T
authors.lastname: Opriessnig
authors.affiliation: Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, MS, USA.
authors.forename: Amelia R
authors.initials: AR
authors.lastname: Woolums
authors.affiliation: Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Hurley
authors.affiliation: Department of Biomedical Sciences and Pathobiology, College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
authors.forename: Xiang-Jin
authors.initials: XJ
authors.lastname: Meng
authors.affiliation: Blood Systems Research Institute, San Francisco, CA, USA; Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA, USA. Electronic address: delwarte@medicine.ucsf.edu.
authors.forename: Eric
authors.initials: E
authors.lastname: Delwart
mesh_terms: D000818:Animals; D052660:Bocavirus / Q000145:classification / Q000235:genetics; D036022:Capsid Proteins / Q000145:classification / Q000235:genetics; D002417:Cattle / Q000097:blood* / Q000821:virology*; D016679:Genome, Viral / Q000235:genetics*; D005843:Geography; D056186:Metagenomics / Q000379:methods*; D010322:Parvoviridae Infections / Q000662:veterinary / Q000821:virology; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D044967:Serum / Q000821:virology; D013045:Species Specificity; D014481:United States; D017361:Viral Nonstructural Proteins / Q000145:classification / Q000235:genetics
chemical_list: D036022:Capsid Proteins; D017361:Viral Nonstructural Proteins
keywords: Bosavirus; Cell culture; Metagenomics; Parvovirus