title: Correlation of five secretory proteins with the nasopharyngeal carcinoma metastasis and the clinical applications.
abstract: In our previous study, five different secretory proteins, including GSN, ADAMTSL4, CALR, PPIA and TXN, have been identified to be associated with the nasopharyngeal carcinoma (NPC) metastasis.In this work, the 5 proteins were further investigated. Bioinformatics analysis suggested that they might play an important role in the process of NPC development.Western blotting analysis showed that all of these 5 targets could be secreted into extracellular by both high metastatic NPC 5-8F cells and non-metastatic NPC 6-10B cells. Except for GSN, the expressions of ADAMTSL4, CALR, PPIA and TXN proteins in extracts of the 5-8F and 6-10B cells were significantly different (P < 0.05). Thus, the expressions of these 4 differentially expressed proteins were further tested in a cohort of NPC tissue specimens. The results indicated that the expression levels of ADAMTSL4 and TXN were highly correlated with the lymph node and distant metastasis (P<0.05) in NPC patients. Moreover, Enzyme-linked immunosorbent assay (ELISA) was used to investigate the concentrations of the ADAMTSL4 and TXN in serum specimens of NPC patients. The results revealed that serum ADAMTSL4 expression level was closely correlated with lymph node metastasis and clinical stage (P<0.05) in NPC patients, and it was able to discriminate metastasis NPC from non-metastasis NPC with a sensitivity of 75.6% and a specificity of 64.7%. The present data show for the first time that the ADAMTSL4 and TXN may be novel and potential biomarkers for predicting the NPC metastasis.Furthermore, the serum ADAMTSL4 could be a potential serum tumor biomarker for prognosis of NPC.
authors.affiliation: Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Tao
authors.affiliation: Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Long
authors.affiliation: Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Facai
authors.initials: F
authors.lastname: Cui
authors.affiliation: Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Lepan
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Manlin
authors.initials: M
authors.lastname: Xiang
authors.affiliation: Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha, Hunan, China.|Department of Clinical Laboratory, Hunan Cancer Hospital, Changsha, Hunan, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Yunlai
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Shiyang
authors.initials: S
authors.lastname: Qiu
authors.affiliation: The Higher Educational Key Laboratory for Cancer Proteomics and Translational Medicine of Hunan, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yi
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D009362:Neoplasm Metastasis; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor
keywords: ADAMTSL4; metastasis; nasopharyngeal carcinoma; secretory proteins; serum tumor biomarker