title: Identification and monitoring of Korean medicines derived fromCinnamomumspp. by using ITS and DNA marker.
abstract: In this study, we identified and evaluated the genetic relationships amongCinnamomumplants, which are used in traditional medicine. We also attempted to monitor the distribution of traditional medicines derived fromCinnamomum cassiaby using DNA barcoding and a species-specific DNA marker. Plants of the genusCinnamomum, and in particularC. cassia, are commonly used as medicinal herbs in the form of Cinnamomi Ramulus, Cinnamomi Cortex, and Cassiae Cortex Interior. However, it is difficult to distinguish among differentCinnamomumspecies based on morphological features, and so to overcome this limitation, nucleotide sequences of the internal transcribed spacer (ITS) region ofCinnamomumDNA were determined and compared. On the basis of the discrepancy in determined ITS sequences, a 408-bp product, amplified by the primer pair CC F1/CC R3, was developed as aC. cassia-specific DNA marker. Using the developed DNA marker in combination with the ITS 2 nucleotide sequence, we monitored imported and commercially supplied medicinal products derived fromCinnamomumplants in markets in Korean, China, and Japan. The results revealed that most of the specimens monitored were derived fromC. cassia.
authors.affiliation: Department of Herbology, College of Korean Medicine, Wonkwang University, Iksan, 54538 Republic of Korea ; Center for Metabolic Function Regulation, Wonkwang University, Iksan, 54538 Republic of Korea.
authors.forename: Eui Jeong
authors.initials: EJ
authors.lastname: Doh
authors.affiliation: Division of Pharmacology, School of Korean Medicine, Pusan National University, Yangsan, 50612 Republic of Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Division of Biological Sciences, Konkuk University, Seoul, 05029 Republic of Korea.
authors.forename: Seung Eun
authors.initials: SE
authors.lastname: Oh
authors.affiliation: Department of Herbology, College of Korean Medicine, Wonkwang University, Iksan, 54538 Republic of Korea.
authors.forename: Guemsan
authors.identifier: 0000-0003-2049-6640
authors.initials: G
authors.lastname: Lee
keywords: Cassiae Cortex Interior; Cinnamomi Cortex; Cinnamomi Ramulus; Cinnamomum cassia; ITS (Internal transcribed spacer)