title: Analysis of the Surface, Secreted, and Intracellular Proteome ofPropionibacterium acnes.
abstract: Propionibacterium acnes, plays an important role in acne vulgaris and other diseases. However, understanding of the exact mechanisms ofP. acnespathogenesis is limited. Few studies have investigated its proteome, which is essential for vaccine development. Here, we comprehensively investigate the proteome ofP. acnesstrain ATCC 6919, including secreted, cell wall, membrane, and cytosolic fractions in three types of growth media. A total of 531 proteins were quantified using an Orbitrap mass spectrometer and bioinformatically categorized for localization and function. Several, including PPA1939, a highly expressed surface and secreted protein, were identified as potential vaccine candidates.
authors.affiliation: Division of Dermatology and Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA; University of California, Irvine School of Medicine, Irvine, CA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Division of Dermatology and Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA.
authors.forename: Jackson
authors.initials: J
authors.lastname: Champer
authors.affiliation: Division of Dermatology and Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA; Department of Dermatology, Greater Los Angeles Healthcare System Veterans Affairs, Los Angeles, CA.
authors.forename: Jenny
authors.initials: J
authors.lastname: Kim
keywords: Propionibacterium acnes; acne; cell wall; mass spectrometry; proteomics