title: The effect of polymeric formula on enterocyte differentiation.
abstract: Exclusive enteral nutrition is established as an initial therapy to induce remission in active Crohn's disease (CD), especially in children, but the mechanisms of action of this therapy are yet to be fully defined. Intestinal alkaline phosphatase (IAP), a recognised marker of enterocyte differentiation, is implicated in the innate gut immune response to enteric pathogens. Using the Caco-2 human adenocarcinoma cell line, this study showed that the incubation of human cells with a polymeric formula (PF) resulted in a dose-dependent increase in the expression of IAP on the cell surface. While further investigation is required to determine the pathway(s) involved, this finding suggests that cell surface-associated IAP may be an aspect of the gut's innate immune response to pathogenic bacteria that is strengthened by PF in the setting of CD.
authors.affiliation: 1 Department of Surgery, University of Otago Christchurch, Christchurch, New Zealand.
authors.forename: Gabrielle R
authors.initials: GR
authors.lastname: Budd
authors.affiliation: 1 Department of Surgery, University of Otago Christchurch, Christchurch, New Zealand.
authors.forename: Alan
authors.initials: A
authors.lastname: Aitchison
authors.affiliation: 2 Department of Pediatrics, University of Otago Christchurch, Christchurch, New Zealand.
authors.forename: Andrew S
authors.initials: AS
authors.lastname: Day
authors.affiliation: 1 Department of Surgery, University of Otago Christchurch, Christchurch, New Zealand.
authors.forename: Jacqueline I
authors.initials: JI
authors.lastname: Keenan
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism*; D018938:Caco-2 Cells; D002241:Carbohydrates / Q000494:pharmacology*; D002364:Caseins / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D003424:Crohn Disease / Q000628:therapy*; D004750:Enteral Nutrition; D020895:Enterocytes / Q000187:drug effects* / Q000502:physiology; D005526:Food, Formulated; D058851:GPI-Linked Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007113:Immunity, Innate; D008055:Lipids / Q000494:pharmacology*; D014674:Plant Proteins, Dietary / Q000494:pharmacology*; D012074:Remission Induction
chemical_list: D002241:Carbohydrates; D002364:Caseins; D058851:GPI-Linked Proteins; D008055:Lipids; D014674:Plant Proteins, Dietary; C080652:Osmolite; C063088:ALPI protein, human; D000469:Alkaline Phosphatase
keywords: Crohn’s disease; cellular differentiation; enteral nutrition; intestinal alkaline phosphatase; vitamin D receptor