title: Description of two novel members of the family Erysipelotrichaceae: Ileibacterium valens gen. nov., sp. nov. and Dubosiella newyorkensis, gen. nov., sp. nov., from the murine intestine, and emendation to the description of Faecalibaculum rodentium.
abstract: To better characterize murine intestinal microbiota, a large number (187) of Gram-positive-staining, rod- and coccoid-shaped, and facultatively or strictly anaerobic bacteria were isolated from small and large intestinal contents from mice. Based on 16S rRNA gene sequencing, a total 115 isolates formed three phylogenetically distinct clusters located within the family Erysipelotrichaceae. Group 1, as represented by strain NYU-BL-A3T, was most closely related to Allobaculum stercoricanis, with 16S rRNA gene sequence similarity values of 87.7 %. A second group, represented by NYU-BL-A4T, was most closely related to Faecalibaculum rodentium, with 86.6 % 16S rRNA gene sequence similarity. A third group had a nearly identical 16S rRNA gene sequence (99.9 %) compared with the recently described Faecalibaculum rodentium, also recovered from a laboratory mouse; however, this strain had a few differences in biochemical characteristics, which are detailed in an emended description. The predominant (>10 %) cellular fatty acids of strain NYU-BL-A3T were C16 : 0 and C18 : 0, and those of strain NYU-BL-A4T were C10 : 0, C16 : 0, C18 : 0 and C18 : 1ω9c. The two groups could also be distinguished by multiple biochemical reactions, with the group represented by NYU-BL-A4T being considerably more active. Based on phylogenetic, biochemical and chemotaxonomic criteria, two novel genera are proposed, Ileibacterium valens gen. nov., sp. nov. with NYU-BL-A3T (=ATCC TSD-63T=DSM 103668T) as the type strain and Dubosiella newyorkensis gen. nov., sp. nov. with NYU-BL-A4T (=ATCC TSD-64T=DSM 103457T) as the type strain.
authors.affiliation: Department of Microbiology, NYU Langone Medical Center, New York, NY 10016, USA.|Department of Medicine, NYU Langone Medical Center, New York, NY 10016, USA.|Department of Neurology, Ann Romney Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Laura M
authors.initials: LM
authors.lastname: Cox
authors.affiliation: Department of Medicine, NYU Langone Medical Center, New York, NY 10016, USA.
authors.forename: Jiho
authors.initials: J
authors.lastname: Sohn
authors.affiliation: R. M. Alden Research Laboratory, Culver City, CA 90230, USA.
authors.forename: Kerin L
authors.initials: KL
authors.lastname: Tyrrell
authors.affiliation: R. M. Alden Research Laboratory, Culver City, CA 90230, USA.
authors.forename: Diane M
authors.initials: DM
authors.lastname: Citron
authors.affiliation: Department of Microbiology and Plant Biology, University of Oklahoma, Norman, USA.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Lawson
authors.affiliation: Department of Microbiology and Plant Biology, University of Oklahoma, Norman, USA.
authors.forename: Nisha B
authors.initials: NB
authors.lastname: Patel
authors.affiliation: Department of Medicine, NYU Langone Medical Center, New York, NY 10016, USA.
authors.forename: Tadasu
authors.initials: T
authors.lastname: Iizumi
authors.affiliation: Department of Medicine, NYU Langone Medical Center, New York, NY 10016, USA.|Department of Microbiology, NYU Langone Medical Center, New York, NY 10016, USA.
authors.forename: Guillermo I
authors.initials: GI
authors.lastname: Perez-Perez
authors.affiliation: R. M. Alden Research Laboratory, Culver City, CA 90230, USA.
authors.forename: Ellie J C
authors.initials: EJC
authors.lastname: Goldstein
authors.affiliation: Department of Medicine, NYU Langone Medical Center, New York, NY 10016, USA.|Department of Microbiology, NYU Langone Medical Center, New York, NY 10016, USA.|New York Harbor Department of Veterans Affairs Medical Center, New York, NY 10010, USA.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Blaser
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D000070036:Faecalibacterium / Q000145:classification*; D005227:Fatty Acids / Q000737:chemistry; D007422:Intestines / Q000382:microbiology*; D051379:Mice / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D008973:Tenericutes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S