title: Upregulation of long noncoding RNA TUG1 promotes cervical cancer cell proliferation and migration.
abstract: Long noncoding RNAs (lncRNAs), a novel class of transcripts that have critical roles in carcinogenesis and progression, have emerged as important gene expression modulators. Recent evidence indicates that lncRNA taurine-upregulated gene 1 (TUG1) functions as an oncogene in numerous types of human cancers. However, its function in the development of cervical cancer remains unknown. The aim of this research was to investigate the clinical significance and biological functions of TUG1 in cervical cancer. TUG1 was found to be significantly upregulated in cervical cancer tissues and four cervical cancer cell lines by quantitative real-time polymerase chain reaction (qRT-PCR). Elevated TUG1 expression was correlated with larger tumor size, advanced international federation of gynecology and obstetrics (FIGO) stage, poor differentiation, and lymph node metastasis. Furthermore, knockdown of TUG1 suppressed cell proliferation with activation of apoptosis, in part by regulating the expression of Bcl-2 and caspase-3. Silencing of TUG1 inhibited cell migration and invasion via the progression of epithelial-mesenchymal transition (EMT). Taken together, our findings indicate that TUG1 acts as an oncogene in cervical cancer and may represent a novel therapeutic target.
authors.affiliation: Department of Obstetrics and Gynecology, Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of General Surgery, First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.
authors.forename: Xiangwei
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of General Surgery, First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.
authors.forename: Chenchen
authors.initials: C
authors.lastname: Mao
authors.affiliation: Department of Microbiology and Immunology, Institute of Molecular Virology and Immunology, Institute of Tropical Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.
authors.forename: Gangqiang
authors.initials: G
authors.lastname: Guo
authors.affiliation: Department of Microbiology and Immunology, Institute of Molecular Virology and Immunology, Institute of Tropical Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.
authors.forename: Sisi
authors.initials: S
authors.lastname: Ye
authors.affiliation: Department of General Surgery, First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.
authors.forename: Ruanmin
authors.initials: R
authors.lastname: Zou
authors.affiliation: Department of Microbiology and Immunology, Institute of Molecular Virology and Immunology, Institute of Tropical Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Obstetrics and Gynecology, Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.
authors.forename: Ledan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Duan
authors.affiliation: Department of Microbiology and Immunology, Institute of Molecular Virology and Immunology, Institute of Tropical Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Xue
mesh_terms: D053148:Caspase 3 / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D008207:Lymphatic Metastasis; D009367:Neoplasm Staging; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D062085:RNA, Long Noncoding / Q000235:genetics*; D015854:Up-Regulation; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology*
chemical_list: C000595707:BCL2 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D062085:RNA, Long Noncoding; C000598536:TUG1 long noncoding RNA, human; C505407:CASP3 protein, human; D053148:Caspase 3
keywords: Bcl-2; LncRNA; TUG1; cervical cancer; epithelial-mesenchymal transition