title: DNA-duplex linker for AFM-SELEX of DNA aptamer against human serum albumin.
abstract: DNA-duplex interactions in thymines and adenins are used as a linker for the novel methodology of Atomic Force Microscope-Systematic Evolution of Ligands by EXpotential enrichment (AFM-SELEX). This study used the hydrogen bonds in 10 mer of both thymines (T10) and adenines (A10). Initially, the interactive force in T10-A10 was measured by AFM, which returned an average interactive force of approximately 350pN. Based on this result, DNA aptamers against human serum albumin could be selected in the 4th round, and 15 different clones could be sequenced. The lowest dissociation constant of the selected aptamer was identified via surface plasmon resonance, and it proved to be identical to that of the commercial aptamer. Therefore, specific hydrogen bonds in DNA can be useful linkers for AFM-SELEX.
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Rokkoudaichou, Nada-ku, Kobe 657 8501, Japan.
authors.forename: Musashi
authors.initials: M
authors.lastname: Takenaka
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Rokkoudaichou, Nada-ku, Kobe 657 8501, Japan.
authors.forename: Yuzo
authors.initials: Y
authors.lastname: Okumura
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Rokkoudaichou, Nada-ku, Kobe 657 8501, Japan.
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Amino
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Rokkoudaichou, Nada-ku, Kobe 657 8501, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Miyachi
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Rokkoudaichou, Nada-ku, Kobe 657 8501, Japan. Electronic address: ochiaki@port.kobe-u.ac.jp.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ogino
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Rokkoudaichou, Nada-ku, Kobe 657 8501, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D004247:DNA / Q000737:chemistry*; D006801:Humans; D006860:Hydrogen Bonding; D018625:Microscopy, Atomic Force / Q000379:methods*; D052156:SELEX Aptamer Technique / Q000379:methods*; D012709:Serum Albumin / Q000737:chemistry*; D020349:Surface Plasmon Resonance
chemical_list: D012709:Serum Albumin; D004247:DNA
keywords: Atomic force microscope; DNA aptamer; Human serum albumin; SELEX