title: Jasmonate Regulates Plant Responses to Postsubmergence Reoxygenation through Transcriptional Activation of Antioxidant Synthesis.
abstract: Submergence induces hypoxia in plants; exposure to oxygen following submergence, termed reoxygenation, produces a burst of reactive oxygen species. The mechanisms of hypoxia sensing and signaling in plants have been well studied, but how plants respond to reoxygenation remains unclear. Here, we show that reoxygenation in Arabidopsis (Arabidopsis thaliana) involves rapid accumulation of jasmonates (JAs) and increased transcript levels of JA biosynthesis genes. Application of exogenous methyl jasmonate improved tolerance to reoxygenation in wild-type Arabidopsis; also, mutants deficient in JA biosynthesis and signaling were very sensitive to reoxygenation. Moreover, overexpression of the transcription factor geneMYC2enhanced tolerance to posthypoxic stress, andmyc2knockout mutants showed increased sensitivity to reoxygenation, indicating that MYC2 functions as a key regulator in the JA-mediated reoxygenation response. MYC2 transcriptionally activates members of theVITAMIN C DEFECTIVE(VTC) andGLUTATHIONE SYNTHETASE(GSH) gene families, which encode rate-limiting enzymes in the ascorbate and glutathione synthesis pathways. Overexpression ofVTC1andGSH1in themyc2-2mutant suppressed the posthypoxic hypersensitive phenotype. The JA-inducible accumulation of antioxidants may alleviate oxidative damage caused by reoxygenation, improving plant survival after submergence. Taken together, our findings demonstrate that JA signaling interacts with the antioxidant pathway to regulate reoxygenation responses in Arabidopsis.
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, Collaborative Innovation Center of Genetics and Development, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Li-Bing
authors.identifier: 0000-0001-5303-8676
authors.initials: LB
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, Collaborative Innovation Center of Genetics and Development, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Yang-Shuo
authors.initials: YS
authors.lastname: Dai
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, Collaborative Innovation Center of Genetics and Development, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, Collaborative Innovation Center of Genetics and Development, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Lu-Jun
authors.initials: LJ
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, Collaborative Innovation Center of Genetics and Development, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, Collaborative Innovation Center of Genetics and Development, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Yong-Xia
authors.initials: YX
authors.lastname: Lai
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, Collaborative Innovation Center of Genetics and Development, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Yi-Cong
authors.initials: YC
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, Collaborative Innovation Center of Genetics and Development, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Le
authors.initials: L
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, Collaborative Innovation Center of Genetics and Development, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Qin-Fang
authors.initials: QF
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, Collaborative Innovation Center of Genetics and Development, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China xiaoshi3@mail.sysu.edu.cn.
authors.forename: Shi
authors.identifier: 0000-0002-6632-8952
authors.initials: S
authors.lastname: Xiao
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics; D000975:Antioxidants / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism* / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D005981:Glutathione Synthase / Q000235:genetics / Q000378:metabolism; D007101:Immersion; D009154:Mutation; D010100:Oxygen / Q000378:metabolism* / Q000494:pharmacology; D054883:Oxylipins / Q000378:metabolism* / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000378:metabolism / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015533:Transcriptional Activation; D014867:Water / Q000378:metabolism
chemical_list: D000975:Antioxidants; D029681:Arabidopsis Proteins; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D003517:Cyclopentanes; C473611:MYC2 protein, Arabidopsis; D054883:Oxylipins; D010937:Plant Growth Regulators; D014867:Water; C011006:jasmonic acid; D005981:Glutathione Synthase; D005978:Glutathione; D001205:Ascorbic Acid; D010100:Oxygen