title: Dual-acting biofunctionalised scaffolds for applications in regenerative medicine.
abstract: Off the shelf scaffolds for replacing ultra-small diameter vascular grafts are valuable for reconstruction of diseased or damaged vessels. The limitations for such grafts include optimal handling with ready availability of varied lengths of grafts, graft patency with the ability to replace the function of active cellular mechanisms and adequate mechanical properties to maintain physicochemical function. We used a well-established, solvent casting method for potential tissue replacement scaffold fabrication with incorporated bioactive molecules, which we have previously explored to confer haemocompatibility. These grafts were tested in-vivo within the abdominal aorta of 10 Wistar rats and the patency was clinically and echographically evaluated. Haemocompatibility and endothelialisation were assessed on explants. Biofunctionalised scaffolds were also grafted subcutaneously and intraperitoneally to evaluate integration, inflammation and angiogenesis reactions. The potential wider applications of this dual acting scaffold were evaluated for its interactions with human dermal fibroblasts as well as bronchial epithelial cells. Physicochemical property evaluation of the functionalised grafts has clarified the mechanical strength and permeability. This study confirmed the microsurgical suturability of tubular grafts and graft patency of functionalized scaffolds. The study demonstrated the potential of a dual acting biofunctionalised scaffold's use for a wide range of tissue engineering applications where micro-porous, yet impermeable scaffolds are needed.
authors.affiliation: Division of Surgery and Interventional Science, University College London, London, UK.|Université Pierre et Marie Curie, 4 Place Jussieu, Paris, 75005, France.|Ecole de Chirurgie, AGEPS, AP-HP, 7 Rue du Fer À Moulin, Paris, 75005, France.
authors.forename: Camilo
authors.initials: C
authors.lastname: Chaves
authors.affiliation: Division of Surgery and Interventional Science, University College London, London, UK.
authors.forename: Chuanyu
authors.initials: C
authors.lastname: Gao
authors.affiliation: Division of Surgery and Interventional Science, University College London, London, UK.
authors.forename: Jerome
authors.initials: J
authors.lastname: Hunckler
authors.affiliation: Division of Surgery and Interventional Science, University College London, London, UK.|University College London Hospitals, London, UK.
authors.forename: Moustafa
authors.initials: M
authors.lastname: Elsawy
authors.affiliation: Ecole de Chirurgie, AGEPS, AP-HP, 7 Rue du Fer À Moulin, Paris, 75005, France.
authors.forename: Josette
authors.initials: J
authors.lastname: Legagneux
authors.affiliation: Institut Cochin-INSERM U1016, 27 rue du fbg Saint Jacques, Paris, 75014, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Renault
authors.affiliation: Hôpital St Antoine, Service de Chirurgie Orthopédique & Traumatologique, Unité Chirurgie Réparatrice & Chirurgie de la Main, Paris, F-75571, France.
authors.forename: Alain Charles
authors.initials: AC
authors.lastname: Masquelet
authors.affiliation: Division of Surgery and Interventional Science, University College London, London, UK. demelach@gmail.com.
authors.forename: Achala
authors.initials: A
authors.lastname: de Mel
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal / Q000473:pathology*; D001807:Blood Vessel Prosthesis; D001980:Bronchi / Q000166:cytology; D004847:Epithelial Cells / Q000166:cytology; D004867:Equipment Design; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007249:Inflammation; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D010539:Permeability; D011108:Polymers / Q000737:chemistry; D011312:Pressure; D051381:Rats; D017208:Rats, Wistar; D044968:Regenerative Medicine / Q000379:methods*; D012867:Skin / Q000378:metabolism; D013314:Stress, Mechanical; D013696:Temperature; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011108:Polymers