title: A ratiometric fluorescence probe for imaging sulfur dioxide derivatives in the mitochondria of living cells.
abstract: Although sulfur dioxide (SO2) plays an essential role in several physiological processes, monitoring of intracellular SO2at subcellular levels remains challenging due to the lack of rapid and sensitive methods for its quantification in a 100% aqueous solution. Herein, a new hemicyanine dyes-based fluorescence probe, NBD-Id, was designed and synthesized for the detection of SO2derivatives in pure aqueous solution and living cells. By virtue of a specific 1,4-addition reaction of SO32-HSO3-and the polymethine chain of hemicyanine, significant changes in the absorption and fluorescence emission spectra were observed in less than 20 seconds. The ratiometric fluorescence (F467/F593) detection of SO2derivatives was then obtained with high sensitivity (detection limit 3.6 nM). It was noted that NBD-Id has a specific response towards SO2derivatives without interference from other anions and biomolecules. Intracellular fluorescence imaging indicated that NBD-Id is cell membrane permeable and mainly distributed within the mitochondria. Therefore, ratiometric fluorescence imaging of SO2derivatives in the mitochondria of MCF-7 cells was successfully demonstrated.
authors.affiliation: School of Chemical Engineering, University of Science and Technology Liaoning, 185 Qianshan Zhong Road, Anshan 114044, P. R. China. zhangzhiqiang@ustl.edu.cn.
authors.forename: Yongfei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Chemical Engineering, University of Science and Technology Liaoning, 185 Qianshan Zhong Road, Anshan 114044, P. R. China. zhangzhiqiang@ustl.edu.cn.
authors.forename: Qingtao
authors.initials: Q
authors.lastname: Meng
authors.affiliation: School of Chemical Engineering, University of Science and Technology Liaoning, 185 Qianshan Zhong Road, Anshan 114044, P. R. China. zhangzhiqiang@ustl.edu.cn and Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, 4072, Australia. r.zhang@uq.edu.au.
authors.forename: Run
authors.identifier: http://orcid.org/0000-0002-0943-824X
authors.initials: R
authors.lastname: Zhang
authors.affiliation: School of Chemical Engineering, University of Science and Technology Liaoning, 185 Qianshan Zhong Road, Anshan 114044, P. R. China. zhangzhiqiang@ustl.edu.cn.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Jia
authors.affiliation: School of Chemical Engineering, University of Science and Technology Liaoning, 185 Qianshan Zhong Road, Anshan 114044, P. R. China. zhangzhiqiang@ustl.edu.cn.
authors.forename: Xuehu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Chemical Engineering, University of Science and Technology Liaoning, 185 Qianshan Zhong Road, Anshan 114044, P. R. China. zhangzhiqiang@ustl.edu.cn.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D061986:MCF-7 Cells; D008928:Mitochondria / Q000737:chemistry*; D015394:Molecular Structure; D061848:Optical Imaging; D013458:Sulfur Dioxide / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D013458:Sulfur Dioxide