title: Effect of Combinatory Treatment With Resveratrol and Guggulsterone on Mild Acute Pancreatitis in Mice.
abstract: This study was conducted to assess the preventive/therapeutic effects of combined administration of resveratrol and guggulsterone on cerulein-induced acute pancreatitis in mice.
  
  
  
  Acute pancreatitis was induced by intraperitoneal injection of cerulein in mice. Serum amylase assay and histology were performed to measure the severity of pancreatitis. Western blotting and multiplex cytokine/chemokine analysis were conducted to understand the action mechanisms of the reagents.
  
  
  
  Serum amylase assay and histology revealed that the severity of acute pancreatitis was reduced by the combinatory treatment with resveratrol and guggulsterone, but the ratio of the band intensity implied that reduced nuclear factor-κB activation is primarily responsible for the effect. The reduced amounts of keratinocyte chemoattractant (chemokine [C-X-C motif] ligand 1), interferon gamma-induced protein 10 (C-X-C motif chemokine 10) and interleukin 6 expression in the sera could be involved in attenuated immune cell migration and reduced inflammation by these reagents.
  
  
  
  Combinatory treatment with resveratrol and guggulsterone marginally reduced cerulein-induced mild acute pancreatitis in mice.
authors.affiliation: From the *Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine; †Department of Internal Medicine and Liver Research Institute, Seoul National University Hospital, Seoul National University College of Medicine, Jongno-gu, Seoul; ‡Department of Internal Medicine, Kangwon National University School of Medicine, Kangwon National University Hospital, Chuncheon; and §Department of Laboratory Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
authors.forename: Nayoung
authors.initials: N
authors.lastname: Kim
authors.forename: Jin Myung
authors.initials: JM
authors.lastname: Park
authors.forename: Sang Hyub
authors.initials: SH
authors.lastname: Lee
authors.forename: Bo Hye
authors.initials: BH
authors.lastname: Kim
authors.forename: Jun Hyuk
authors.initials: JH
authors.lastname: Son
authors.forename: Ji Kon
authors.initials: JK
authors.lastname: Ryu
authors.forename: Yong-Tae
authors.initials: YT
authors.lastname: Kim
authors.forename: Woochang
authors.initials: W
authors.lastname: Lee
mesh_terms: D000208:Acute Disease; D000681:Amylases / Q000097:blood; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D002108:Ceruletide; D018925:Chemokines / Q000097:blood; D016207:Cytokines / Q000097:blood; D005260:Female; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism; D010195:Pancreatitis / Q000139:chemically induced / Q000188:drug therapy*; D011282:Pregnenediones / Q000494:pharmacology*; D000077185:Resveratrol; D050796:STAT3 Transcription Factor / Q000378:metabolism; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D018925:Chemokines; D016207:Cytokines; D016328:NF-kappa B; D011282:Pregnenediones; D050796:STAT3 Transcription Factor; D013267:Stilbenes; D002108:Ceruletide; C023617:pregna-4,17-diene-3,16-dione; D000681:Amylases; D000077185:Resveratrol