title: Comparative proteomic analysis of liver antioxidant mechanisms in Megalobrama amblycephala stimulated with dietary emodin.
abstract: Oxidative stress is a toxicological endpoint that correlates with the nutrition status of fish through cellular damage, inflammation, and apoptosis. In order to understand the antioxidant mechanism induced by dietary emodin in Megalobrama amblycephala liver, a comparative proteomic analysis was performed to investigate the proteome alteration under emodin administration. 27 altered protein spots were separated under 30 mg kg-1emodin stimulation based on 2-DE, and were all successfully identified using MALDI-TOF/TOF, representing 17 unique proteins. These proteins were functionally classified into antioxidant, metabolism, cytoskeleton, chaperone, signal transduction and cofactor groups. Network interaction and Gene Ontology annotation indicated 10 unique proteins were closely related to antioxidation and directly regulated by each other. Compared with the control group, administration of 30 mg kg-1emodin significantly increased the antioxidant-related mRNA expressions of GPx1, GSTm and HSP70, but decreased the mRNA expressions of GAPDH and Sord, which was consistent with the protein expression. Nevertheless, Pgk1 and Aldh8a1 were up- and down-regulated, and ALDOB was down- and up-regulated at the mRNA and protein levels, respectively. These results revealed that the altered proteins enhanced antioxidation via complex regulatory mechanisms, and 30 mg kg-1emodin was a suitable immunostimulant for M. amblycephala.
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China.
authors.forename: Changyou
authors.initials: C
authors.lastname: Song
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China.|Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China.|Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xie
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China.|Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China.
authors.forename: Xianping
authors.initials: X
authors.lastname: Ge
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China.
authors.forename: Zhenxin
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China.
authors.forename: Mingchun
authors.initials: M
authors.lastname: Ren
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China.
authors.forename: Qunlan
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China.
authors.forename: Linghong
authors.initials: L
authors.lastname: Miao
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China.|Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China.
authors.forename: Pao
authors.initials: P
authors.lastname: Xu
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lin
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D003530:Cyprinidae / Q000235:genetics; D004032:Diet; D004642:Emodin / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000235:genetics*; D006801:Humans; D007223:Infant; D008099:Liver / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics*; D020543:Proteome / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D013482:Superoxide Dismutase / Q000235:genetics; D000094765:Glutathione Peroxidase GPX1
chemical_list: D000975:Antioxidants; D020543:Proteome; D012333:RNA, Messenger; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D004642:Emodin; D000094765:Glutathione Peroxidase GPX1