title: Photoinduced Cross-Linking of Short Furan-Modified DNA on Surfaces.
abstract: We report for the first time the formation of site-specific interstrand cross-linked (ICL) surface-immobilized furan-modified DNA duplexes via singlet oxygen.1O2, necessary for effecting furan-mediated ICL formation, was produced in situ using methylene blue or a zinc phthalocyanine derivative (TT1) as a photosensitizer. Via surface plasmon resonance spectroscopy, we show that surface ICL was achieved, and a robust link formed that enhances the stability of the 12-mer duplex even after surface regeneration. The described method represents a novel platform technology based on surfaces with addressable and stable DNA duplexes requiring only short oligonucleotides.
authors.affiliation: Department of Analytical Chemistry; Reference Materials, BAM Federal Institute for Materials Research and Testing , Richard-Willstätter-Str. 11, D-12489 Berlin, Germany.|Department of Organic Chemistry, University Autónoma of Madrid , Cantoblanco, 28049 Madrid, Spain.
authors.forename: Cinthya
authors.initials: C
authors.lastname: Véliz Montes
authors.affiliation: Branch Bioanalytics and Bioprocesses, IZI-BB Fraunhofer Institute for Cell Therapy and Immunology , Am Mühlenberg 11, D-14476 Potsdam, Germany.
authors.forename: Henry
authors.initials: H
authors.lastname: Memczak
authors.affiliation: Faculty of Sciences, Department of Organic and Macromolecular Chemistry, Organic and Biomimetic Chemistry Research Group, Ghent University , Krijgslaan 281 (S4), 9000 Ghent, Belgium.
authors.forename: Ellen
authors.initials: E
authors.lastname: Gyssels
authors.affiliation: Department of Organic Chemistry, University Autónoma of Madrid , Cantoblanco, 28049 Madrid, Spain.|IMDEA Nanoscience , c/Faraday, 9, Cantoblanco, 28049 Madrid, Spain.
authors.forename: Tomás
authors.initials: T
authors.lastname: Torres
authors.affiliation: Faculty of Sciences, Department of Organic and Macromolecular Chemistry, Organic and Biomimetic Chemistry Research Group, Ghent University , Krijgslaan 281 (S4), 9000 Ghent, Belgium.
authors.forename: Annemieke
authors.identifier: 0000-0003-0179-7608
authors.initials: A
authors.lastname: Madder
authors.affiliation: Department of Analytical Chemistry; Reference Materials, BAM Federal Institute for Materials Research and Testing , Richard-Willstätter-Str. 11, D-12489 Berlin, Germany.
authors.forename: Rudolf J
authors.identifier: 0000-0003-2228-1248
authors.initials: RJ
authors.lastname: Schneider
mesh_terms: D003432:Cross-Linking Reagents / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D019793:Fluorescein / Q000737:chemistry; D005663:Furans / Q000737:chemistry*; D017879:Isothiocyanates / Q000737:chemistry; D055668:Photochemical Processes; D026082:Singlet Oxygen / Q000737:chemistry; D020349:Surface Plasmon Resonance; D013499:Surface Properties
chemical_list: D003432:Cross-Linking Reagents; D005663:Furans; D017879:Isothiocyanates; D026082:Singlet Oxygen; C037152:isothiocyanic acid; D004247:DNA; D019793:Fluorescein; C039281:furan