title: Copper(II)-Controlled Molecular Glue for Mismatched DNA.
abstract: Isothermal hybridization of two DNA strands bearing three thymine-thymine (T:T) mismatches can be brought about in the presence of a stoichiometric amount of a bis-naphthalene macrocycle, 2,7-BisNP-NH. This process can be reverted by addition of a CuIIsalt due to formation of a dinuclear metal complex which does not bind to DNA. Subsequent sequestration of CuIIreleases the macrocycle and restores the hybridization state of DNA strands, thus allowing implementation of a fast fluorescent two-state DNA switch.
authors.affiliation: CNRS UMR9187, INSERM U1196, Institut Curie, PSL Research University, 91405, Orsay, France.|CNRS UMR9187, INSERM U1196, Université Paris Sud, Université Paris-Saclay, 91405, Orsay, France.
authors.forename: Naoko
authors.initials: N
authors.lastname: Kotera
authors.affiliation: Institut de Chimie Moléculaire et des Matériaux d'Orsay (ICMMO), CNRS UMR8182, Université Paris Sud, Université Paris-Saclay, 91405, Orsay, France.
authors.forename: Régis
authors.identifier: 0000-0002-9003-0670
authors.initials: R
authors.lastname: Guillot
authors.affiliation: CNRS UMR9187, INSERM U1196, Institut Curie, PSL Research University, 91405, Orsay, France.|CNRS UMR9187, INSERM U1196, Université Paris Sud, Université Paris-Saclay, 91405, Orsay, France.
authors.forename: Marie-Paule
authors.identifier: 0000-0002-2053-7513
authors.initials: MP
authors.lastname: Teulade-Fichou
authors.affiliation: CNRS UMR9187, INSERM U1196, Institut Curie, PSL Research University, 91405, Orsay, France.|CNRS UMR9187, INSERM U1196, Université Paris Sud, Université Paris-Saclay, 91405, Orsay, France.
authors.forename: Anton
authors.identifier: 0000-0002-0424-0461
authors.initials: A
authors.lastname: Granzhan
mesh_terms: D020137:Base Pair Mismatch; D056831:Coordination Complexes / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004492:Edetic Acid / Q000737:chemistry; D007364:Intercalating Agents / Q000737:chemistry*; D008024:Ligands; D047028:Macrocyclic Compounds / Q000737:chemistry*; D009281:Naphthalenes / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D010472:Perchlorates / Q000737:chemistry*; D013696:Temperature; D013941:Thymine / Q000737:chemistry
chemical_list: D056831:Coordination Complexes; D007364:Intercalating Agents; D008024:Ligands; D047028:Macrocyclic Compounds; D009281:Naphthalenes; D010472:Perchlorates; C062280:copper(II) perchlorate; D003300:Copper; D004247:DNA; D004492:Edetic Acid; D013941:Thymine
keywords: DNA recognition; DNA switch; hybridization; macrocycles; metal complexes