title: Whole Blood Transcriptome Sequencing Reveals Gene Expression Differences between Dapulian and Landrace Piglets.
abstract: There is little genomic information regarding gene expression differences at the whole blood transcriptome level of different pig breeds at the neonatal stage. To solve this, we characterized differentially expressed genes (DEGs) in the whole blood of Dapulian (DPL) and Landrace piglets using RNA-seq (RNA-sequencing) technology. In this study, 83 DEGs were identified between the two breeds. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses identified immune response and metabolism as the most commonly enriched terms and pathways in the DEGs. Genes related to immunity and lipid metabolism were more highly expressed in the DPL piglets, while genes related to body growth were more highly expressed in the Landrace piglets. Additionally, the DPL piglets had twofold more single nucleotide polymorphisms (SNPs) and alternative splicing (AS) than the Landrace piglets. These results expand our knowledge of the genes transcribed in the piglet whole blood of two breeds and provide a basis for future research of the molecular mechanisms underlying the piglet differences.
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong 271000, China.
authors.forename: Jiaqing
authors.identifier: 0000-0002-8753-4034
authors.initials: J
authors.lastname: Hu
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong 271000, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong 271000, China.
authors.forename: Wei
authors.identifier: 0000-0002-2901-5156
authors.initials: W
authors.lastname: Chen
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong 271000, China.
authors.forename: Chuanhao
authors.initials: C
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong 271000, China.
authors.forename: Yandong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong 271000, China.
authors.forename: Yongqing
authors.identifier: 0000-0002-0740-2470
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong 271000, China.
authors.forename: Hui
authors.identifier: 0000-0002-9432-6233
authors.initials: H
authors.lastname: Wang
mesh_terms: D017398:Alternative Splicing / Q000502:physiology*; D000818:Animals; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D020641:Polymorphism, Single Nucleotide; D013045:Species Specificity; D013552:Swine; D059467:Transcriptome / Q000502:physiology*