title: Reconstruction of a composite comparative map composed of ten legume genomes.
abstract: The Fabaceae (legume family) is the third largest and the second of agricultural importance among flowering plant groups. In this study, we report the reconstruction of a composite comparative map composed of ten legume genomes, including seven species from the galegoid clade (Medicago truncatula,Medicago sativa,Lens culinaris, Pisum sativum,Lotus japonicus,Cicer arietinum,Vicia faba) and three species from the phaseoloid clade (Vigna radiata,Phaseolus vulgaris,Glycine max). To accomplish this comparison, a total of 209 cross-species gene-derived markers were employed. The comparative analysis resulted in a single extensive genetic/genomic network composed of 93 chromosomes or linkage groups, from which 110 synteny blocks and other evolutionary events (e.g., 13 inversions) were identified. This comparative map also allowed us to deduce several large scale evolutionary events, such as chromosome fusion/fission, with which might explain differences in chromosome numbers among compared species or between the two clades. As a result, useful properties of cross-species genic markers were re-verified as an efficient tool for cross-species translation of genomic information, and similar approaches, combined with a high throughput bioinformatic marker design program, should be effective for applying the knowledge of trait-associated genes to other important crop species for breeding purposes. Here, we provide a basic comparative framework for the ten legume species, and expect to be usefully applied towards the crop improvement in legume breeding.
authors.affiliation: Department of Medical Bioscience, Graduate School, Dong-A University, Nakdong-Daero 550-Beongil 37, Saha-Gu, Busan, 49315 Republic of Korea.
authors.forename: Chaeyoung
authors.initials: C
authors.lastname: Lee
authors.affiliation: Department of Molecular Genetics, College of Natural Resources and Life Science, Dong-A University, Nakdong-Daero 550-Beongil 37, Saha-Gu, Busan, 49315 Republic of Korea.|SeqGenesis Inc., Gajeongbuk-Ro 96, Yuseong-Gu, Daejeon, 34111 Republic of Korea.
authors.forename: Dongwoon
authors.initials: D
authors.lastname: Yu
authors.affiliation: Department of Molecular Genetics, College of Natural Resources and Life Science, Dong-A University, Nakdong-Daero 550-Beongil 37, Saha-Gu, Busan, 49315 Republic of Korea.
authors.forename: Hong-Kyu
authors.initials: HK
authors.lastname: Choi
authors.affiliation: Korea Bioinformation Center, Korea Research Institute of Bioscience & Biotechnology, Gwahak-Ro 125 Yuseong-Gu, Daejeon, 34141 Republic of Korea.
authors.forename: Ryan W
authors.initials: RW
authors.lastname: Kim
keywords: Comparative genomics; Gene-specific marker; Legumes; Synteny