title: miR-574-3p acts as a tumor promoter in osteosarcoma by targeting SMAD4 signaling pathway.
abstract: Human osteosarcoma is the most common primary bone malignancy sarcoma that affects primarily children and people <20 years old. In the present study, it was demonstrated that miR-574-3p was downregulated in human osteosarcoma U2OS, SAOS and MG63 cells lines as well as in osteosarcoma tissue compared with the normal tissues. Downregulation of miR-574-3p by antisense miR-574-3p, inhibited cell growth and induced cell apoptosis. Overexpression of miR-574-3p by transfection with miR-574-3p mimics promoted the growth of U2OS cells. The present study then identified mothers against decapentaplegic homolog 4 (SMAD4) as a target of miR-574-3p and SMAD4 was suppressed in miR-574-3p transfected cells. Overexpression of SMAD4 could rescue the promoting effects of miR-574-3p on cancer cell growth. In conclusion, miR-574-3p exerts tumor-promoting roles by targeting the tumor-suppressing gene SMAD4 and its downstream signaling in human osteosarcoma, which provides a novel target for the treatment.
authors.affiliation: Department of Orthopedics of Jinling Hospital, Nanjing University, School of Medicine, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Haidong
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Orthopedics of Jinling Hospital, Nanjing University, School of Medicine, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Xiaozhou
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Orthopedics of Jinling Hospital, Nanjing University, School of Medicine, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Orthopedics of Jinling Hospital, Nanjing University, School of Medicine, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Orthopedics of Jinling Hospital, Nanjing University, School of Medicine, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Jianning
authors.initials: J
authors.lastname: Zhao
keywords: SMAD4; miR-574-3p; oncogene; osteosarcoma