title: Photocurrent Generation of Reconstituted Photosystem II on a Self-Assembled Gold Film.
abstract: Photosystem II (PSII)-modified gold electrodes were prepared by the deposition of PSII reconstituted with platinum nanoparticles (PtNPs) on Au electrodes. PtNPs modified with 1-[15-(3,5,6-trimethyl-1,4-benzoquinone-2-yl)]pentadecyl disulfide ((TMQ(CH2)15S)2) were incorporated into the QBsite of PSII isolated from thermophilic cyanobacterium Thermosynechococcus elongatus. The reconstitution was confirmed by QA-reoxidation measurements. PSII reconstituted with PtNPs was deposited and integrated on a Au(111) surface modified with 4,4'-biphenyldithiol. The cross section of the reconstituted PSII film on the Au electrode was investigated by SEM. Absorption spectra showed that the surface coverage of the electrode was about 18 pmol PSII cm-2. A photocurrent density of 15 nAcm-2at E = +0.10 V (vs Ag/AgCl) was observed under 680 nm irradiation. The photoresponse showed good reversibility under alternating light and dark conditions. Clear photoresponses were not observed in the absence of PSII and molecular wire. These results supported the photocurrent originated from PSII and moved to a gold electrode by light irradiation, which also confirmed conjugation with orientation through the molecular wire.
authors.affiliation: Department of Chemistry, School of Science, The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Miyachi
authors.affiliation: Department of Chemistry, School of Science, The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Shu
authors.initials: S
authors.lastname: Ikehira
authors.affiliation: Department of Chemistry, School of Science, The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Daiki
authors.initials: D
authors.lastname: Nishiori
authors.affiliation: Department of Chemistry, School of Science, The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Yoshinori
authors.identifier: 0000-0002-6155-2357
authors.initials: Y
authors.lastname: Yamanoi
authors.affiliation: Department of Biology, Faculty of Science, Tokyo University of Science , Kagurazaka 1-3, Shinjuku-ku, Tokyo 162-8601, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Yamada
authors.affiliation: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology , Yokohama 226-8503, Japan.
authors.forename: Masako
authors.initials: M
authors.lastname: Iwai
authors.affiliation: Department of Biology, Faculty of Science, Tokyo University of Science , Kagurazaka 1-3, Shinjuku-ku, Tokyo 162-8601, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Tomo
authors.affiliation: Controlled Photobiosynthesis Laboratory, Institute of Plant Physiology, Russian Academy of Sciences , Botanicheskaya Street 35, Moscow 127276, Russia.|Institute of Basic Biological Problems, Russian Academy of Sciences , Pushchino, Moscow Region 142290, Russia.|Faculty of Biology, M. V. Lomonosov Moscow State University , Leninskie Gory 1-12, Moscow 119991, Russia.
authors.forename: Suleyman I
authors.initials: SI
authors.lastname: Allakhverdiev
authors.affiliation: Department of Chemistry, School of Science, The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nishihara