title: Construction and characterisation of a heparan sulphate heptasaccharide microarray.
abstract: A targeted heptasaccharide library was synthesised to prepare a heparan sulphate (HS) microarray. The array was probed with two glycan-binding proteins, HS 3-O-sulphotransferase 1 and antithrombin, demonstrating the binding selectivity between HS and proteins. The HS microarray technique will accelerate the understanding of the structure and function relationships of HS.
authors.affiliation: Division of Chemical Biology and Medicinal Chemistry, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC 27599, USA. jian_liu@unc.edu and College of Environmental & Safety Engineering, Changzhou University, Changzhou 213164, Jiangsu, China.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Yang
authors.affiliation: Division of Chemical Biology and Medicinal Chemistry, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC 27599, USA. jian_liu@unc.edu.
authors.forename: Po-Hung
authors.initials: PH
authors.lastname: Hsieh
authors.affiliation: Department of Chemistry and Chemical Biology, Centre for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
authors.forename: Xinyue
authors.initials: X
authors.lastname: Liu
authors.affiliation: Division of Chemical Biology and Medicinal Chemistry, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC 27599, USA. jian_liu@unc.edu and School of Chinese Medical Material, Guangzhou University of Chinese Medicine, Guangzhou, 510006, Guangdong, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Department of Chemistry and Chemical Biology, Centre for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Chemistry and Chemical Biology, Centre for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Division of Chemical Biology and Medicinal Chemistry, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC 27599, USA. jian_liu@unc.edu.
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Chemistry and Chemical Biology, Centre for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
authors.forename: Fuming
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Chemistry and Chemical Biology, Centre for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
authors.forename: Robert J
authors.identifier: http://orcid.org/0000-0003-2219-5833
authors.initials: RJ
authors.lastname: Linhardt
authors.affiliation: Division of Chemical Biology and Medicinal Chemistry, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC 27599, USA. jian_liu@unc.edu.
authors.forename: Jian
authors.identifier: http://orcid.org/0000-0001-8552-1400
authors.initials: J
authors.lastname: Liu
mesh_terms: D006497:Heparan Sulfate / Q000737:chemistry*; D046228:Microarray Analysis; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D006497:Heparan Sulfate; D009844:Oligosaccharides