title: High-throughput untargeted screening of veterinary drug residues and metabolites in tilapia using high resolution orbitrap mass spectrometry.
abstract: An analytical method was developed and validated for simultaneous analysis of one hundred and thirty-seven veterinary drug residues and metabolites from sixteen different classes in tilapia utilizing an improved fully non-targeted way of data acquisition with fragmentation. The automated on-line extraction procedure was achieved in a simple disposable pipet extraction. Ultrahigh-performance liquid chromatography and electrospray ionization quadrupole Orbitrap high-resolution mass spectrometry (UHPLC Q-Orbitrap) was used for the separation and detection of all the analytes. The methodology was validated by taking into consideration the guidelines specified in European SANCO/12571/2013 Guideline 2013 and Commission Decision 2002/657/EC. The extraction recoveries ranged from 81% to 111%. The limits of decision ranged from 0.01 to 2.73 μg kg-1and the detection capabilities ranged from 0.01 to 4.73 μg kg-1. The one hundred and thirty-seven compounds behave dynamic 0.1-500 μg kg-1, with correlation coefficient >0.99. The fully non-targeted data acquisition way improves both sensitivity and selectivity for the fragments, which is beneficial for screening performance and identification capability. This validated method has been successfully applied on screening of veterinary drug residues and metabolites in muscle of tilapia, an important and intensively produced fish in aquaculture.
authors.affiliation: School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi'an 710021, China; Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China. Electronic address: foodjiawei@aliyun.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Jia
authors.affiliation: School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi'an 710021, China; Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Chu
authors.affiliation: Thermo Fisher Scientific, 355 River Oaks Parkway, San Jose, CA 95134, United States.
authors.forename: James
authors.initials: J
authors.lastname: Chang
authors.affiliation: U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, 5100 Paint Branch Parkway, HFS-717, College Park, MD 20740-3835, United States.
authors.forename: Perry G
authors.initials: PG
authors.lastname: Wang
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D057166:High-Throughput Screening Assays; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D017210:Tilapia; D019155:Veterinary Drugs / Q000032:analysis*
chemical_list: D019155:Veterinary Drugs
keywords: Aquaculture; Multi-residue; UHPLC/ESI Q-Orbitrap; Veterinary drug residues