title: Influence of feeding habits in the endocrine pancreas of insectivore bat Pteronotus personatus and nectarivore bat Anoura geoffroyi: A comparative stereological and immunohistochemical study.
abstract: Pteronotus personatus as an insectivore bat and has a diet that consists of a high protein diet, whereas the diet of Anoura geoffroyi, a predominantly nectarivore bat, is rich in simple sugars like sucrose, glucose and fructose. Considering that diet influences the activation of different pathways, which may influence morphological adaptations in the gastrointestinal system, the aim of this study was to compare the morphology of the endocrine pancreas in P. personatus and A. geoffroyi. For this, histological, stereological and immunohistochemical methods were used. In P. personatus, the average diameter of the pancreatic islet was 40.47μm±13.94, while in A. geoffroyi was 88.16μm±36.40. The total number of pancreatic islets in P. personatus was 26150±2346 and in A. geoffroyi was 15970±1666. In P. personatus, the volume density of the pancreatic islets was 3.4%± 2.6, whereas in A. geoffroyi the volume density was 6.1%±3.7. In addition, the immunodensity of the α, β and δ cells, in P. personatus was 25.8%±11.9, 35.5%±13.5, 3.9%±0.7, respectively, and in A. geoffroyi was 33.10%±12.7, 55.08%±7.4, 6.2%±4.6, respectively. In conclusion, the results of this study indicate differences in the pancreatic weight/body, weight ratio, diameter and volume density of pancreatic islets and in immunodensity of the β and α cells between both species, which have different dietary habits.
authors.affiliation: Laboratory of Teaching and Research in Histology and Embryology, Biomedical Institute, Fluminense Federal University, RJ, Brazil. Electronic address: claricemachado@id.uff.br.
authors.forename: Clarice
authors.initials: C
authors.lastname: Machado-Santos
authors.affiliation: Laboratory of Morphometry, Metabolism & Cardiovascular Disease, Biomedical Center, Institute of Biology, State University of Rio de Janeiro, RJ, Brazil.
authors.forename: Júlio Cesar Fraulob
authors.initials: JC
authors.lastname: Aquino
authors.affiliation: Conservation Biology Laboratory, Department of Ecology, Federal University of Sergipe, SE, Brazil.
authors.forename: Patrício Adriano
authors.initials: PA
authors.lastname: da Rocha
authors.affiliation: Animal Biology Program, Rio de Janeiro Federal Rural University, RJ, Brazil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Abidu-Figueiredo
authors.affiliation: Laboratory of Integrative Histology, Research Program in Glycobiology, Rio de Janeiro Federal University, RJ, Brazil.
authors.forename: Lycia
authors.initials: L
authors.lastname: de Brito-Gitirana
authors.affiliation: Animal Biology Program, Rio de Janeiro Federal Rural University, RJ, Brazil.
authors.forename: Armando
authors.initials: A
authors.lastname: Sales
mesh_terms: D000818:Animals; D002685:Chiroptera / Q000033:anatomy & histology* / Q000502:physiology; D004032:Diet; D007312:Eulipotyphla / Q000033:anatomy & histology* / Q000502:physiology; D005247:Feeding Behavior / Q000502:physiology; D041981:Gastrointestinal Tract / Q000033:anatomy & histology / Q000502:physiology; D006184:Habits; D060434:Herbivory / Q000502:physiology; D007515:Islets of Langerhans / Q000033:anatomy & histology* / Q000502:physiology
keywords: Chiroptera; Endocrine cells; Histology; Pancreatic islets