title: Polyphyllin I inhibits the growth of ovarian cancer cells in nude mice.
abstract: Polyphyllin I (PPI) is an active component in Rhizoma Paridis, which displays extensive pharmacological antitumor activities. In a previous study, we found that polyphyllin I exhibited inhibitory effects on cell growth in the human ovarian cancer HO-8910PM cell line, as well as promoting apoptosis and the inhibition of cell migration. Furthermore, gene expression was also profiled by microarray, which showed that numerous genes were altered by PPI; three genes were of particular note that were associated with tumor progression, namely, Caspase-9, C-jun and Wnt5a. In the present study, the effect of PPI on subcutaneous tumor growth (HO-8910PM cells) in nude mice was further evaluated, and immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR) were used to examine the expression of Caspase-9, C-jun and Wnt5a in subcutaneous and lung metastatic tumor tissues, in order to investigate the possible mechanisms involved. The results showed that PPI significantly inhibited the tumor growthin vivowithout a marked impact on body weight, and through use of immunohistochemical staining and RT-PCR, it was found that the expression of Caspase-9 and Wnt5a was decreased, while the expression of C-jun was increased, in subcutaneous and lung metastatic tumor tissue; this was consistent with thein vitroresults. In conclusion, the present study showed that PPI exerted antitumor activity on ovarian cancer cellsin vivo, and indicated that the modulation of Caspase-9, C-jun and Wnt5a may be involved in the antitumor effect of PPI.
authors.affiliation: Research Institute, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China; Key Laboratory of Diagnosis and Treatment Technology on Thoracic Oncology, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China.
authors.forename: Linhui
authors.initials: L
authors.lastname: Gu
authors.affiliation: Research Institute, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China; Key Laboratory of Diagnosis and Treatment Technology on Thoracic Oncology, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Feng
authors.affiliation: Research Institute, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China; Key Laboratory of Diagnosis and Treatment Technology on Thoracic Oncology, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: Biological Specimen Bank, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Xu
authors.affiliation: Biological Specimen Bank, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yu
keywords: C-jun; Caspase-9; Wnt5a; growth; ovarian cancer; polyphyllin I