title: IL-6 and IL-10 gene polymorphisms in patients with aggressive periodontitis: effects on GCF, serum and clinic parameters.
abstract: Genetic variations observed in cytokines affect periodontitis susceptibility. The aim of this study was to investigate interleukin(IL)-6(-174) and IL-10(-597) gene polymorphisms in generalized aggressive periodontitis (GAgP) patients. Also, we aimed to evaluate the effects of IL-6 and IL-10 gene polymorphisms on the clinical outcomes of non-surgical periodontal therapy and cytokine levels in gingival crevicular fluid(GCF) and serum. Fifty-three patients with GAgP and 50 periodontally healthy individuals were included in this study. Clinical parameters, GCF and blood samples were collected at baseline and at 6-week. Non-surgical periodontal therapy was performed in patients with GAgP. Gene analysis were determined by PCR-RFLP(polymerase chain reaction-restriction fragment length polymorphism) and cytokine levels were determined by enzyme-linked immunosorbent assay(ELISA).GAgP patients showed significant improvement on clinical parameters after periodontal therapy(p<0.05). In the GAgP group, IL-6 GG genotype and G allele frequency were higher than in the control group. GCF IL-6 level was also significantly lower at 6-week in the GAgP group. Higher GCF IL-10 levelswere observed in patients carrying the IL-6 GG genotype than in those carrying the GC+CC genotype at baseline. In conclusion, IL-6(-174) and IL-10(-597) gene polymorphisms were found to be associated with GAgP and genotype distribution did not affect the outcome of non-surgical periodontal therapy, while patients with IL-6(-174) GG genotype had higher levels of GCF IL-10 levels.
authors.affiliation: Cumhuriyet University Faculty of Dentistry, Department of Periodontology, Sivas, Turkey.
authors.forename: Emine
authors.initials: E
authors.lastname: Pirim Gorgun
authors.affiliation: Cumhuriyet University Faculty of Dentistry, Department of Periodontology, Sivas, Turkey.
authors.forename: Hulya
authors.initials: H
authors.lastname: Toker
authors.affiliation: Cumhuriyet University Faculty of Sciences, Department of Molecular Biology and Genetics, Sivas, Turkey.
authors.forename: Ertan Mahir
authors.initials: EM
authors.lastname: Korkmaz
authors.affiliation: Cumhuriyet University Faculty of Medicine, Department of Microbiology, Sivas, Turkey.
authors.forename: Omer
authors.initials: O
authors.lastname: Poyraz
mesh_terms: D000328:Adult; D010520:Aggressive Periodontitis / Q000235:genetics* / Q000628:therapy; D016022:Case-Control Studies; D003774:Dental Plaque Index; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005883:Gingival Crevicular Fluid / Q000737:chemistry; D006801:Humans; D016753:Interleukin-10 / Q000032:analysis* / Q000235:genetics*; D015850:Interleukin-6 / Q000032:analysis* / Q000235:genetics*; D016015:Logistic Models; D008297:Male; D010512:Periodontal Index; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012016:Reference Values; D013997:Time Factors; D055815:Young Adult
chemical_list: D015850:Interleukin-6; D016753:Interleukin-10