title: Structure of the O-specific polysaccharide from the lipopolysaccharide of Aeromonas hydrophila strain K691 containing 4-acetamido-4,6-dideoxy-d-glucose.
abstract: The O-specific polysaccharide (OPS) was isolated from the lipopolysaccharide of Aeromonas hydrophila strain K691 and studied by chemical methods and1H and13C NMR spectroscopy, including 2D1H,1H COSY, TOCSY, NOESY,1H-detected heteronuclear1H,13C HSQC, and HMBC experiments. It was found that the O-specific polysaccharide was built up of pentasaccharide repeating units composed of β-GlcpNAc, 2-O-acetylated α-Rhap, and β-Quip4NAc residues. The following structure of the OPS was established: →3)-α-l-Rha2OAc-(1→3)-β-d-GlcNAc-(1→3)-α-l-Rha2OAc-(1→3)-β-d-GlcNAc-(1→2)-β-d-Qui4NAc-(1→.
authors.affiliation: Department of Genetics and Microbiology, M. Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Pakiet
authors.affiliation: Department of Genetics and Microbiology, M. Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland. Electronic address: aturska@hektor.umcs.lublin.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Turska-Szewczuk
authors.affiliation: Department of Genetics and Microbiology, M. Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Magdalena A
authors.initials: MA
authors.lastname: Karas
authors.affiliation: Department of Fish Diseases, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Pekala
authors.affiliation: Department of Genetics and Microbiology, M. Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Hubert
authors.initials: H
authors.lastname: Pietras
mesh_terms: D000117:Acetylglucosamine / Q000737:chemistry*; D016980:Aeromonas hydrophila / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy
chemical_list: D008070:Lipopolysaccharides; D000117:Acetylglucosamine
keywords: 4-Amino-4,6-dideoxy-d-glucose; Aeromonas hydrophila; Fish pathogen; LPS; O-Acetylation; O-Antigen; O-specific polysaccharide