title: Genome Analysis of a Novel Broad Host Range Proteobacteria Phage Isolated from a Bioreactor Treating Industrial Wastewater.
abstract: Bacteriophages are viruses that infect bacteria, and consequently they have a major impact on the development of a microbial population. In this study, the genome of a novel broad host range bacteriophage,Aquamicrobiumphage P14, isolated from a wastewater treatment plant, was analyzed. TheAquamicrobiumphage P14 was found to infect members of different Proteobacteria classes (Alphaproteobacteria and Betaproteobacteria). This phage contains a 40,551 bp long genome and 60% of its genes had blastx hits. Furthermore, the bacteriophage was found to share more than 50% of its genes with several podoviruses and has the same gene order as other polyvalent bacteriophages. The results obtained in this study led to the conclusion that indeed general features of the genome of theAquamicrobiumphage P14 are shared with other broad host range bacteriophages, however further analysis of the genome is needed in order to identify the specific mechanisms which enable the bacteriophage to infect both Alphaproteobacteria and Betaproteobacteria.
authors.affiliation: Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben-Gurion University of the Negev, P.O. Box 653, Be'er Sheva 8410501, Israel. deleeuw@post.bgu.ac.il.
authors.forename: Marina
authors.initials: M
authors.lastname: de Leeuw
authors.affiliation: Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben-Gurion University of the Negev, P.O. Box 653, Be'er Sheva 8410501, Israel. becherm@post.bgu.ac.il.
authors.forename: Maayan
authors.initials: M
authors.lastname: Baron
authors.affiliation: Unit of Environmental Engineering, Faculty of Engineering Sciences, Ben-Gurion University of the Negev, Be'er Sheva 8410501, Israel. brenner@bgu.ac.il.
authors.forename: Asher
authors.initials: A
authors.lastname: Brenner
authors.affiliation: Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben-Gurion University of the Negev, P.O. Box 653, Be'er Sheva 8410501, Israel. arielkus@bgu.ac.il.|The National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be'er Sheva 8410501, Israel. arielkus@bgu.ac.il.|The Ilse Katz Center for Meso and Nanoscale Science and Technology, Ben-Gurion University of the Negev, Be'er Sheva 8410501, Israel. arielkus@bgu.ac.il.
authors.forename: Ariel
authors.initials: A
authors.lastname: Kushmaro
keywords: Aquamicrobium; bacteriophage; genome; polyvalent; wastewater