title: Jagged 2 silencing inhibits motility and invasiveness of colorectal cancer cell lines.
abstract: Although the Notch pathway has been reported to be activated in colorectal cancer (CRC), limited information is available regarding the expression and role of its ligand, Jagged 2 (JAG2), in CRC. Using immunohistochemistry, the present study demonstrated that JAG2 protein expression may be detected in up to 95% of CRC cases and is 3-fold upregulated in tumor cells compared to surrounding normal tissues. This finding suggests that JAG2 may have a role in the tumorigenicity of CRC. To further investigate the cellular functions of JAG2 expression in CRC, two different small interfering RNAs (siRNAs) were used to downregulate JAG2 expression in CRC cell lines (HCT116, DLD-1 and HT-29). The results indicated that JAG2 knockdown inhibits the motility and invasiveness of CRC cell lines without significantly affecting cell proliferation. These findings implicate JAG2 in promoting aggressiveness of CRC, and lay the foundation for its future development as a therapeutic target for the treatment of CRC.
authors.affiliation: Department of Oncology, Shenzhen People's Hospital, Second Clinical Medical College of Jinan University, Shenzhen, Guangdong 518029, P.R. China.
authors.forename: Wan
authors.initials: W
authors.lastname: He
authors.affiliation: State Key Laboratory of Oncology in South China, Sir YK Pao Center for Cancer, Department of Clinical Oncology, Hong Kong Cancer Institute and Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong SAR, P.R. China.
authors.forename: Charles Ming Lok
authors.initials: CM
authors.lastname: Chan
authors.affiliation: Department of Health Technology and Informatics, Hong Kong Polytechnic University, Hong Kong SAR, P.R. China.
authors.forename: Sze Chuen Cesar
authors.initials: SC
authors.lastname: Wong
authors.affiliation: State Key Laboratory of Oncology in South China, Sir YK Pao Center for Cancer, Department of Clinical Oncology, Hong Kong Cancer Institute and Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong SAR, P.R. China.
authors.forename: Thomas Chi Chuen
authors.initials: TC
authors.lastname: Au
authors.affiliation: State Key Laboratory of Oncology in South China, Sir YK Pao Center for Cancer, Department of Clinical Oncology, Hong Kong Cancer Institute and Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong SAR, P.R. China.
authors.forename: Wing Shan
authors.initials: WS
authors.lastname: Ho
authors.affiliation: Department of Pathology, Queen Elizabeth Hospital, Hong Kong SAR, P.R. China.
authors.forename: Amanda Kit Ching
authors.initials: AK
authors.lastname: Chan
authors.affiliation: State Key Laboratory of Oncology in South China, Sir YK Pao Center for Cancer, Department of Clinical Oncology, Hong Kong Cancer Institute and Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong SAR, P.R. China.
authors.forename: Andrew Sai Kit
authors.initials: AS
authors.lastname: Chan
authors.affiliation: State Key Laboratory of Oncology in South China, Sir YK Pao Center for Cancer, Department of Clinical Oncology, Hong Kong Cancer Institute and Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong SAR, P.R. China.
authors.forename: Brigette Buig Yue
authors.initials: BB
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Oncology in South China, Sir YK Pao Center for Cancer, Department of Clinical Oncology, Hong Kong Cancer Institute and Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong SAR, P.R. China.
authors.forename: Anthony Tak Cheung
authors.initials: AT
authors.lastname: Chan
keywords: Jagged 2; Notch; colorectal cancer; invasion; migration