title: Snapshot situation of oxidative degradation of the nervous system, kidney, and adrenal glands biomarkers-neuroprostane and dihomo-isoprostanes-urinary biomarkers from infancy to elderly adults.
abstract: We analyzed biomarkers of lipid peroxidation of the nervous system -F2-dihomo-isoprostanes, F3-neuroprostanes, and F4-neuroprostanes- in urine samples from 158 healthy volunteers ranging from 4 to 88 years old with the aim of analyzing possible associations between their excretion values and age (years). Ten biomarkers were screened in the urine samples by UHPLC-QqQ-MS/MS. Four F2-dihomo-isoprostanes (ent-7-(R)-7-F2t-dihomo-isoprostane, ent-7-epi-7-F2t-dihomo-isoprostane, 17-F2t-dihomo-isoprostane, 17-epi-17-F2t-dihomo-isoprostane), and one DPA-neuroprostane (4-F3t-neuroprostane) were detected in the samples. On the one hand, we found a significant, positive correlation (Rho: 0.197, P=0.015) between the age increase and the amount of total F2-dihomo-IsoPs. On the other hand, the values were significantly higher in the childhood group (4-12 years old), when compared to the adolescence group (13-17 years old) and the young adult group (18-35 years old). Surprisingly, no significant differences were found between the middle-aged adults (36-64 years old) and the elderly adults (65-88 years old). We display a snapshot situation of excretory values of oxidative stress biomarkers of the nervous system, using healthy volunteers representative of the different stages of human growth and development. The values reported in this study could be used as a basal or starting point in clinical interventions related to aging processes and/or pathologies associated with the nervous system.
authors.affiliation: Department of Food Science and Technology, CEBAS-CSIC, Campus de Espinardo, P.O. Box 164, 30100 Murcia, Spain.
authors.forename: Libia Alejandra
authors.initials: LA
authors.lastname: García-Flores
authors.affiliation: Department of Food Science and Technology, CEBAS-CSIC, Campus de Espinardo, P.O. Box 164, 30100 Murcia, Spain. Electronic address: smescudero@cebas.csic.es.
authors.forename: Sonia
authors.initials: S
authors.lastname: Medina
authors.affiliation: Clinical Analysis Service, University Hospital Virgen de la Arrixaca, Murcia, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Martínez-Hernández
authors.affiliation: Institut des Biomolécules Max Mousseron, UMR 5247 CNRS-University of Montpellier, ENSCM, Montpellier, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Oger
authors.affiliation: Institut des Biomolécules Max Mousseron, UMR 5247 CNRS-University of Montpellier, ENSCM, Montpellier, France.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Galano
authors.affiliation: Institut des Biomolécules Max Mousseron, UMR 5247 CNRS-University of Montpellier, ENSCM, Montpellier, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Durand
authors.affiliation: Clinical Analysis Service, University Hospital Virgen de la Arrixaca, Murcia, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Casas-Pina
authors.affiliation: Department of Food Science and Technology, CEBAS-CSIC, Campus de Espinardo, P.O. Box 164, 30100 Murcia, Spain.
authors.forename: Federico
authors.initials: F
authors.lastname: Ferreres
authors.affiliation: Department of Food Science and Technology, CEBAS-CSIC, Campus de Espinardo, P.O. Box 164, 30100 Murcia, Spain. Electronic address: angelgil@cebas.csic.es.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Gil-Izquierdo
mesh_terms: D000293:Adolescent; D000311:Adrenal Glands / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D028441:F2-Isoprostanes / Q000378:metabolism; D005260:Female; D006801:Humans; D007668:Kidney / Q000378:metabolism; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D009420:Nervous System / Q000378:metabolism*; D058632:Neuroprostanes / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D053719:Tandem Mass Spectrometry; D055815:Young Adult
chemical_list: D015415:Biomarkers; D028441:F2-Isoprostanes; D058632:Neuroprostanes
keywords: Age; Dihomo-isoprostanes; Lipid peroxidation; Neuroprostanes; Oxidative stress; Urine biomarkers