title: The mechanism of alopolysaccharide protecting ulceralive colitis.
abstract: The aim of this study is to explain the mechanism of alopolysaccharide protecting ulceralive colitis in cells and animal models. We divided this study into two parts: cell and animal research parts. In the cell research, HT-29 cells were divided into normal group, model group, aloe polysacchride group and positive drug group, the cell model was used by tumor necrosis factor- α (TNF-α) combine with LPS, detecting IL-6 concentration of difference groups by Elisa testing, Apoptosis of each group was detected by flow cytometry. We detected JAK2 and STAT-3 gene expressions of difference groups by RT-PCR. WB assay was used to detect the expression of JAK2, p-JAK2, STAT-3 and p-STAT3 protein in the cells of each group. In animal experiment, SD rats were divided into normal group, model control group, aloe polysaccharide group and positive drug group. A rat model of colitis was established with 2,4,6- three nitrobenzene sulfonic acid (TNBS). IL-6 concentrations of difference groups were measured by Elisa test, and compared the colon length of difference groups, H&E staining observation of colon tissue changes in each group. We observed JAK2, p-JAK2, STAT-3 and p-STAT3 protein expression in colon tissues by immuno histochemistry and measured JAK2 and STAT-3 gene expression of difference groups by RT-PCR. We found IL-6 concentration and cell apoptosis rate of Model group were significantly up-regulation compared with normal group (P<0.05, respectively) in the cell experiment. Compared with Model group, The IL-6 concentration and apoptosis rate of aloe polysaccharide group and positive drug group were significantly down-regulation (P<0.05, respectively). In the gene expression, JAK2 and STAT-3 expression of aloe polysaccharide group and positive drug group were higher than model group (P<0.05, respectively). JAK2, p-JAK2, STAT-3 and p-STAT3 protein expression of Aloe polysaccharide group and positive drug group were lower than model group. In animal experiment, compared with model group, The serum IL-6 concentration of aloe polysaccharide group and positive drug group were significantly decreased (P<0.05, respectively), colon length was improved by drug treated, The HE stain of aloe polysaccharide and positive drug group were significantly improved. In immuno histochemistry, The JAK2, p-JAK2, STAT-3 and p-STAT3 protein expression of aloe polysaccharide and positive drug group was significantly improved, The JAK2 and STAT-3 gene expression of aloe polysaccharide group and positive drug group were signivicantly lower than those of model group (P<0.05, respectively). Depending on the results, We supposed Aloe polysaccharide could effectively control the apoptosis of colonic tissues by inhibiting the JAK2/STAT-3 signaling pathway in vovo and vitro.
authors.affiliation: Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Nanchang University, No.1 Minde Road, East Lake District, Nanchang City, Jiangxi Province 330006, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Lin
authors.affiliation: Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Nanchang University, No.1 Minde Road, East Lake District, Nanchang City, Jiangxi Province 330006, China. Electronic address: lihonglangsci2016@hotmail.com.
authors.forename: Li
authors.initials: L
authors.lastname: Honglang
authors.affiliation: Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Nanchang University, No.1 Minde Road, East Lake District, Nanchang City, Jiangxi Province 330006, China.
authors.forename: Li
authors.initials: L
authors.lastname: Weifeng
authors.affiliation: Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Nanchang University, No.1 Minde Road, East Lake District, Nanchang City, Jiangxi Province 330006, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Junmin
authors.affiliation: Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Nanchang University, No.1 Minde Road, East Lake District, Nanchang City, Jiangxi Province 330006, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Jiantao
authors.affiliation: Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Nanchang University, No.1 Minde Road, East Lake District, Nanchang City, Jiangxi Province 330006, China.
authors.forename: Gu
authors.initials: G
authors.lastname: Junjing
mesh_terms: D000504:Aloe / Q000737:chemistry*; D000818:Animals; D017209:Apoptosis; D003093:Colitis, Ulcerative / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects; D019073:HT29 Cells; D006801:Humans; D007150:Immunohistochemistry; D015850:Interleukin-6 / Q000378:metabolism; D053614:Janus Kinase 2 / Q000378:metabolism; D008297:Male; D011134:Polysaccharides / Q000494:pharmacology / Q000627:therapeutic use*; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D017207:Rats, Sprague-Dawley; D050796:STAT3 Transcription Factor / Q000378:metabolism
chemical_list: D015850:Interleukin-6; D011134:Polysaccharides; D020011:Protective Agents; D050796:STAT3 Transcription Factor; D053614:Janus Kinase 2
keywords: Aloe polysaccharide; Apoptosis; JAK2/STAT-3 signaling pathway; Ulcerative colitis