title: The effect of lead exposure on fatty acid composition in mouse brain analyzed using pseudo-catalytic derivatization.
abstract: We performed toxicological study of mice exposed to lead by quantifying fatty acids in brain of the mice. This study suggests that the introduced analytical method had an extremely high tolerance against impurities such as water and extractives; thus, it led to the enhanced resolution in visualizing the spectrum of fatty acid profiles in animal brain. Furthermore, one of the biggest technical advantages achieved in this study was the quantitation of fatty acid methyl ester profiles of mouse brain using a trace amount of sample (e.g., 100 μL mixture). Methanol was screened as the most effective extraction solvent for mouse brain. The behavioral test of the mice before and after lead exposure was conducted to see the effect of lead exposure on fatty acid composition of the mice' brain. The lead exposure led to changes in disease-related behavior of the mice. Also, the lead exposure induced significant alterations of fatty acid profile (C16:0, C 18:0, and C 18:1) in brain of the mice, implicated in pathology of psychiatric diseases. The alteration of fatty acid profile of brain of the mice suggests that the derivatizing technique can be applicable to most research fields associated with the environmental neurotoxins with better resolution in a short time, as compared to the current protocols for lipid analysis.
authors.affiliation: Department of Environment and Energy, Sejong University, Seoul 05006, Republic of Korea.
authors.forename: Jong-Min
authors.initials: JM
authors.lastname: Jung
authors.affiliation: Department of Environment and Energy, Sejong University, Seoul 05006, Republic of Korea.
authors.forename: Jechan
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Civil and Environmental Engineering, Hanyang University, Seoul 04763, Republic of Korea.
authors.forename: Ki-Hyun
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Department of Biological Science and Technology, Sejong University, Seoul 05006, Republic of Korea.
authors.forename: In Geon
authors.initials: IG
authors.lastname: Jang
authors.affiliation: Department of Biological Science and Technology, Sejong University, Seoul 05006, Republic of Korea.
authors.forename: Jae Gwang
authors.initials: JG
authors.lastname: Song
authors.affiliation: Department of Anatomy and Cell Biology, School of Medicine, Sungkyunkwan University, Suwon 16419, Republic of Korea.
authors.forename: Kyeongjin
authors.initials: K
authors.lastname: Kang
authors.affiliation: Department of Applied Analytical and Physical Chemistry, Ghent University, Ghent 9000, Belgium.
authors.forename: Filip M G
authors.initials: FMG
authors.lastname: Tack
authors.affiliation: Advanced Technology Department, Land & Housing Institute, Daejon 34047, Republic of Korea.
authors.forename: Jeong-Ik
authors.initials: JI
authors.lastname: Oh
authors.affiliation: Department of Environment and Energy, Sejong University, Seoul 05006, Republic of Korea. Electronic address: ekwon74@sejong.ac.kr.
authors.forename: Eilhann E
authors.initials: EE
authors.lastname: Kwon
authors.affiliation: Department of Biological Science and Technology, Sejong University, Seoul 05006, Republic of Korea. Electronic address: kimhyung@sejong.ac.kr.
authors.forename: Hyung-Wook
authors.initials: HW
authors.lastname: Kim
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001682:Biological Availability; D001921:Brain / Q000187:drug effects* / Q000378:metabolism*; D002384:Catalysis; D004785:Environmental Pollutants / Q000378:metabolism / Q000633:toxicity; D004951:Esterification; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D007854:Lead / Q000378:metabolism* / Q000633:toxicity*; D050356:Lipid Metabolism / Q000187:drug effects; D000432:Methanol; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D004785:Environmental Pollutants; D005227:Fatty Acids; D007854:Lead; D000432:Methanol
keywords: Behavioral test; Fatty acid methyl esters (FAMEs); Lead (Pb); Lipid profile; Transesterification