title: The difference in in vivo sensitivity between Bacillus licheniformis PerR and Bacillus subtilis PerR is due to the different cellular environments.
abstract: PerR, a member of Fur family of metal-dependent regulators, is a major peroxide sensor in many Gram positive bacteria, and controls the expression of genes involved in peroxide resistance. Bacillus licheniformis, a close relative to the well-studied model organism Bacillus subtilis, contains three PerR-like proteins (PerRBL, PerR2 and PerR3) in addition to Fur and Zur. In the present study, we characterized the role of PerRBLin B. licheniformis. In vitro and in vivo studies indicate that PerRBL, like PerRBS, uses either Fe2+or Mn2+as a corepressor and only the Fe2+-bound form of PerRBLsenses low levels of H2O2by iron-mediated histidine oxidation. Interestingly, regardless of the difference in H2O2sensitivity, if any, between PerRBLand PerRBS, B. licheniformis expressing PerRBLor PerRBScould sense lower levels of H2O2and was more sensitive to H2O2than B. subtilis expressing PerRBLor PerRBS. This result suggests that the differences in cellular milieu between B. subtilis and B. licheniformis, rather than the intrinsic differences in PerRBSand PerRBLper se, affect the H2O2sensing ability of PerR inside the cell and the H2O2resistance of cell. In contrast, B. licheniformis and B. subtilis expressing Staphylococcus aureus PerR (PerRSA), which is more sensitive to H2O2than PerRBLand PerRBS, were more resistant to H2O2than those expressing either PerRBLor PerRBS. This result indicates that the sufficient difference in H2O2susceptibility of PerR proteins can override the difference in cellular environment and affect the resistance of cell to H2O2.
authors.affiliation: Department of Life Science and Research Institute for Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Life Science and Research Institute for Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea.
authors.forename: Young-Bin
authors.initials: YB
authors.lastname: Won
authors.affiliation: Department of Life Science and Research Institute for Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea.
authors.forename: Chang-Jun
authors.initials: CJ
authors.lastname: Ji
authors.affiliation: Department of Life Science and Research Institute for Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea.
authors.forename: Yoon-Mo
authors.initials: YM
authors.lastname: Yang
authors.affiliation: Department of Life Science and Research Institute for Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea.
authors.forename: Su-Hyun
authors.initials: SH
authors.lastname: Ryu
authors.affiliation: Department of Life Science and Research Institute for Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea.
authors.forename: Shin-Yeong
authors.initials: SY
authors.lastname: Ju
authors.affiliation: Department of Life Science and Research Institute for Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Kwon
authors.affiliation: Department of Life Science and Research Institute for Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea.
authors.forename: Yeh-Eun
authors.initials: YE
authors.lastname: Lee
authors.affiliation: Department of Life Science and Research Institute for Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea. Electronic address: jwl@hanyang.ac.kr.
authors.forename: Jin-Won
authors.initials: JW
authors.lastname: Lee
mesh_terms: D000069976:Bacillus licheniformis / Q000378:metabolism*; D001412:Bacillus subtilis / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D005454:Fluorescence Polarization; D006639:Histidine / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D010084:Oxidation-Reduction; D012097:Repressor Proteins / Q000378:metabolism*; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins; D012097:Repressor Proteins; C122138:peroxide repressor proteins; D006639:Histidine; D006861:Hydrogen Peroxide; D007501:Iron
keywords: Bacillus licheniformis; Fur (ferric uptake regulator) family; Hydrogen peroxide; PerR (peroxide sensing repressor)