title: Clinical significance of leukocyte-associated immunoglobulin-like receptor-1 expression in human cervical cancer.
abstract: Leukocyte-associated immunoglobulin-like receptor-1 (LAIR-1) is broadly expressed on the majority of immune cells; however, the biological role of LAIR in solid tumors has yet to be elucidated. In the present study, using immunohistochemical staining analysis, the expression of LAIR-1 in human cervical cancer (HCC) and nontumor-adjacent tissue specimens was determined, and the results indicated that the expression of LAIR-1 in HCC tissue was higher compared with that in noncancerous tissue. The χ2test was used to analyze the correlation between the expression of LAIR-1 in tumor tissues with clinicopathological parameters. The results showed that the expression of LAIR-1 in the cancer cell nucleus was significantly associated with tumor size, pathological differentiation, T classification and clinical stage. In addition, the expression in the cytoplasm was evidently associated with the number of positive lymph nodes. The HCC cell line, ME-180, which does not express LAIR-1, was stably transfected using LAIR-1 cDNA. Cell Counting Kit-8 and an annexin V assay showed that the overexpression of LAIR-1 in ME-180 cells suppressed the proliferation and anti-apoptosis capacity of the cells. These findings demonstrated that LAIR-1 is markedly overexpressed in HCC tissue, and that its expression status is associated with tumor progression. LAIR-1 may be a biomarker and target in the diagnosis and treatment of patients with HCC.
authors.affiliation: Department of Immunology, School of Basic Medicine, Binzhou Medical University, Yantai, Shandong 264003, P.R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Immunology, School of Basic Medicine, Binzhou Medical University, Yantai, Shandong 264003, P.R. China.
authors.forename: Xueshan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Immunology, School of Basic Medicine, Binzhou Medical University, Yantai, Shandong 264003, P.R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Miao
authors.affiliation: Department of Immunology, School of Basic Medicine, Binzhou Medical University, Yantai, Shandong 264003, P.R. China.
authors.forename: Yanning
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Immunology, School of Basic Medicine, Binzhou Medical University, Yantai, Shandong 264003, P.R. China.
authors.forename: Jiangnan
authors.initials: J
authors.lastname: Xue
authors.affiliation: Department of Immunology, School of Basic Medicine, Binzhou Medical University, Yantai, Shandong 264003, P.R. China.
authors.forename: Qizhi
authors.initials: Q
authors.lastname: Cao
authors.affiliation: Department of Immunology, School of Basic Medicine, Binzhou Medical University, Yantai, Shandong 264003, P.R. China.
authors.forename: Xiaoshu
authors.initials: X
authors.lastname: Zhang
keywords: biomarker; human cervical cancer; inhibitory receptor; leukocyte-associated immunoglobulin-like receptor-1