title: In vivo metabolomic interpretation of the anti-obesity effects of hyacinth bean (Dolichos lablab L.) administration in high-fat diet mice.
abstract: The esoteric anti-obesity effects of hyacinth bean (Dolichos lablab L) have largely remained unexplored. Herein, we investigated the anti-obesity mechanisms of hyacinth bean compared to milk thistle, a natural herb employed for ameliorating obesity-related diseases, using high-fat diet (HFD) fed mice towards unfolding the perplexing mechanisms.
  
  
  
  C57BL/6J mice were orally administered hyacinth bean (25 mg/kg/day) and milk thistle (100 mg/kg/day) for 9 weeks along with HFD. Intriguingly, a number of anti-obesity mechanisms indexed through clinical parameters, suppression in weight gains and liver steatosis were found similar to some disparity. Furthermore, the corresponding metabolic implications were studied through MS-based metabolite profiling, and using the Kyoto Encyclopedia of Genes and Genomes for metabolic pathways revealing that hyacinth bean or milk thistle administration effectively attenuates the HFD-induced lipid, glucose, and bile acid metabolism, with former specifically attenuates pyruvate-derived amino acids metabolism. Among them, valine, asparagine, and lysine displayed high correlation with blood clinical parameters.
  
  
  
  A lower dose of hyacinth bean resulted in similar anti-obesity effects as milk thistle, as confirmed by both clinical and metabolomics analyses. Equivocally, we conjecture that hyacinth bean could be used as a potent anti-obesity herbal functional food.
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul, Republic of Korea.
authors.forename: Dong Ho
authors.initials: DH
authors.lastname: Suh
authors.affiliation: KM Convergence Research Division,  Korea Institute of Oriental Medicine, Daejeon, Republic of Korea.
authors.forename: Hye Won
authors.initials: HW
authors.lastname: Lee
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul, Republic of Korea.
authors.forename: Eun Sung
authors.initials: ES
authors.lastname: Jung
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul, Republic of Korea.
authors.forename: Digar
authors.initials: D
authors.lastname: Singh
authors.affiliation: Institute of Traditional Medicine and Bioscience, Daejeon University, Daejeon, Republic of Korea.
authors.forename: Seung-Hyung
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul, Republic of Korea.
authors.forename: Choong Hwan
authors.initials: CH
authors.lastname: Lee
mesh_terms: D000818:Animals; D019440:Anti-Obesity Agents / Q000493:pharmacokinetics / Q000494:pharmacology*; D001647:Bile Acids and Salts / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects; D031294:Dolichos / Q000737:chemistry*; D055951:Functional Food; D008401:Gas Chromatography-Mass Spectrometry; D050356:Lipid Metabolism / Q000187:drug effects; D008810:Mice, Inbred C57BL; D020944:Silybum marianum / Q000737:chemistry; D009765:Obesity / Q000178:diet therapy* / Q000209:etiology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D019440:Anti-Obesity Agents; D001647:Bile Acids and Salts; D010936:Plant Extracts
keywords: Amino acids; Anti-obesity effects; Hyacinth bean; Metabolomics; Milk thistle