title: In Vitro Antiproliferative and Antioxidant Effects of Extracts fromRubus caesiusLeaves and Their Quality Evaluation.
abstract: The present study was performed to evaluate the effect of different extracts and subfractions fromRubus caesiusleaves on two human colon cancer cell lines obtained from two stages of the disease progression lines HT29 and SW948. Tested samples inhibited the viability of cells, both HT29 and SW948 lines, in a concentration-dependent manner. The most active was the ethyl acetate fraction which, applied at the highest concentration (250μg/mL), decreased the viability of cells (HT29 and SW948) below 66%. The extracts and subfractions were also investigated for antioxidant activities on DPPH and FRAP assays. All extracts, with the exception of water extract at a dose of 250μg/mL, almost totally reduced DPPH. The highest Fe3+ion reduction was shown for the diethyl and ethyl acetate fractions. It was more than 6.5 times higher (at a dose 250μg/mL) as compared to the control. The LC-MS studies of the analysed preparations showed that all samples contain a wide variety of polyphenolics, among which ellagitannins turned out to be the main constituents with dominant ellagic acid, sanguiin H-6, and flavonol derivatives.
authors.affiliation: Department of Pharmacognosy, Medical University of Białystok, ul. Mickiewicza 2a, 15-230 Białystok, Poland.
authors.forename: Daniel Mirosław
authors.initials: DM
authors.lastname: Grochowski
authors.affiliation: Department of Virology and Immunology, Maria Curie-Skłodowska University, ul. Akademicka 19, 20-033 Lublin, Poland; Department of General Ophthalmology, Medical University of Lublin, ul. Chmielna 1, 20-079 Lublin, Poland.
authors.forename: Roman
authors.initials: R
authors.lastname: Paduch
authors.affiliation: Department of Industrial Microbiology, Maria Curie-Skłodowska University, ul. Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Adrian
authors.initials: A
authors.lastname: Wiater
authors.affiliation: Department of Virology and Immunology, Maria Curie-Skłodowska University, ul. Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Adrianna
authors.initials: A
authors.lastname: Dudek
authors.affiliation: Department of Industrial Microbiology, Maria Curie-Skłodowska University, ul. Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Pleszczyńska
authors.affiliation: Department of Organic Chemistry, Medical University of Białystok, ul. Mickiewicza 2a, 15-222 Białystok, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Tomczykowa
authors.affiliation: Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, ul. Banacha 1, 02-097 Warsaw, Poland.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Granica
authors.affiliation: Department of Industrial Microbiology, Maria Curie-Skłodowska University, ul. Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Polak
authors.affiliation: Department of Pharmacognosy, Medical University of Białystok, ul. Mickiewicza 2a, 15-230 Białystok, Poland.
authors.forename: Michał
authors.identifier: 0000-0002-4063-1048
authors.initials: M
authors.lastname: Tomczyk