title: Vitamin B3metal-organic frameworks as potential delivery vehicles for therapeutic nitric oxide.
abstract: The synthesis and structural characterization of two isostructural metal (M=Ni, Co) 3D framework structure that integrate vitamin B3building blocks with NO delivery capabilities and low toxicity is presented. The compounds with a formula [M2(μ2-H2O)(μ-vitamin B3)4]·2H2O contain two crystallographic distinct divalent metal centres connected by a bridging water and carboxylate group from vitamin B3. The porous compounds have the capability of storing and releasing nitric oxide (NO) in a slow and reversible manner, with released amounts of 2.6 and 2.0μmol NOmgsolid-1, on the Ni and Co compound, respectively. The NO release followed a convenient slow release kinetic profile in both gas and liquid phases. Haemoglobin tests demonstrated that NO is released to the medium in a biologically active form, thus suitable to trigger the desired response in biological systems. The toxicity of the samples with and without loaded NO was evaluated from cytotoxicity tests in HeLa and HEKn cells, showing low toxicity of the compounds at concentrations below 180μgcm-3. The overall results indicate that these bio based MOFs are of interest for therapeutic applications related with NO delivery. STATEMENT OF SIGNIFICANCE.
authors.affiliation: CERENA, Departamento de Engenharia Química, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisboa, Portugal; Centro de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal.
authors.forename: Rosana V
authors.initials: RV
authors.lastname: Pinto
authors.affiliation: Centro de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal.
authors.forename: Fernando
authors.initials: F
authors.lastname: Antunes
authors.affiliation: Centro de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal.
authors.forename: João
authors.initials: J
authors.lastname: Pires
authors.affiliation: Department of Chemistry, CICECO, University of Aveiro, 3810-193 Aveiro, Portugal.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Graça
authors.affiliation: Department of Chemistry, CICECO, University of Aveiro, 3810-193 Aveiro, Portugal. Electronic address: pbrandao@ua.pt.
authors.forename: Paula
authors.initials: P
authors.lastname: Brandão
authors.affiliation: CERENA, Departamento de Engenharia Química, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisboa, Portugal. Electronic address: moises.pinto@tecnico.ulisboa.pt.
authors.forename: Moisés L
authors.initials: ML
authors.lastname: Pinto
mesh_terms: D001672:Biocompatible Materials / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D003198:Computer Simulation; D016503:Drug Delivery Systems; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008670:Metals / Q000737:chemistry*; D009536:Niacinamide / Q000494:pharmacology*; D009569:Nitric Oxide / Q000494:pharmacology* / Q000627:therapeutic use; D009930:Organic Chemicals / Q000737:chemistry*; D010108:Oxyhemoglobins / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D008670:Metals; D009930:Organic Chemicals; D010108:Oxyhemoglobins; D009536:Niacinamide; D009569:Nitric Oxide
keywords: Adsorption; Controlled delivery systems; Metal-organic frameworks; Nitric oxide; Vitamin B(3)