title: Silver-Containing Hydroxyapatite Coating Reduces Biofilm Formation by Methicillin-ResistantStaphylococcus aureusIn Vitro and In Vivo.
abstract: Biofilm-producing bacteria are the principal causes of infections associated with orthopaedic implants. We previously reported that silver-containing hydroxyapatite (Ag-HA) coatings exhibit high antibacterial activity against methicillin-resistantStaphylococcus aureus(MRSA). In the present study, we evaluated the effects of Ag-HA coating of implant surfaces on biofilm formation. Titanium disks (14-mm diameter, 1-mm thickness), one surface of which was coated with HA or 0.5%-3.0% Ag-HA with a thermal spraying technique, were used. In vitro, the disks were inoculated with an MRSA suspension containing 4 × 105CFU and incubated for 1-2 weeks. In vivo, MRSA-inoculated HA and 3% Ag-HA disks (8.8-10.0 × 108CFU) were implanted subcutaneously on the back of rats for 1-7 days. All disks were subsequently stained with a biofilm dye and observed under a fluorescence microscope, and biofilm coverage rates (BCRs) were calculated. The BCRs on the Ag-HA coating were significantly lower than those on the HA coating at all time points in vitro (p< 0.05). Similar results were observed in vivo (p< 0.001) without argyria. Ag-HA coating reduced biofilm formation by MRSA in vitro and in vivo; therefore, Ag-HA coating might be effective for reducing implant-associated infections.
authors.affiliation: Department of Orthopaedic Surgery, Faculty of Medicine, Saga University, Saga 849-8501, Japan.
authors.forename: Masaya
authors.identifier: 0000-0002-4121-9127
authors.initials: M
authors.lastname: Ueno
authors.affiliation: Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga 849-8501, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miyamoto
authors.affiliation: Department of Orthopaedic Surgery, Faculty of Medicine, Saga University, Saga 849-8501, Japan.
authors.forename: Masatsugu
authors.identifier: 0000-0001-6999-9711
authors.initials: M
authors.lastname: Tsukamoto
authors.affiliation: Department of Orthopaedic Surgery, Faculty of Medicine, Saga University, Saga 849-8501, Japan.
authors.forename: Shuichi
authors.identifier: 0000-0002-6005-5914
authors.initials: S
authors.lastname: Eto
authors.affiliation: Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga 849-8501, Japan; Research Department, KYOCERA Medical Corporation, Osaka 532-0003, Japan.
authors.forename: Iwao
authors.identifier: 0000-0001-9511-5473
authors.initials: I
authors.lastname: Noda
authors.affiliation: Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga 849-8501, Japan.
authors.forename: Takeo
authors.initials: T
authors.lastname: Shobuike
authors.affiliation: Department of Orthopaedic Surgery, Faculty of Medicine, Saga University, Saga 849-8501, Japan.
authors.forename: Tomoki
authors.initials: T
authors.lastname: Kobatake
authors.affiliation: Department of Orthopaedic Surgery, Faculty of Medicine, Saga University, Saga 849-8501, Japan.
authors.forename: Motoki
authors.identifier: 0000-0001-7786-8957
authors.initials: M
authors.lastname: Sonohata
authors.affiliation: Department of Orthopaedic Surgery, Faculty of Medicine, Saga University, Saga 849-8501, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Mawatari
mesh_terms: D000818:Animals; D018441:Biofilms / Q000187:drug effects*; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008297:Male; D008422:Materials Testing; D055624:Methicillin-Resistant Staphylococcus aureus / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012834:Silver / Q000737:chemistry / Q000494:pharmacology
chemical_list: D020099:Coated Materials, Biocompatible; D012834:Silver; D017886:Durapatite