title: Using fluorescence immunochromatographic test strips based on quantum dots for the rapid and sensitive determination of microcystin-LR.
abstract: A novel immunosensor for the detection of microcystin-LR (MC-LR) was constructed with use of immunochromatographic test strips (ICTS). Quantum dots were chosen to be the fluorescent labels for the immune sensor in ICTS because of their excellent optical and electronic properties with a relatively narrow emission spectrum. The detection sensitivity of the ICTS was related to the concentration of the fluorescent probe and the amount of the MC-LR standards. Under optimal conditions, with MC-LR as the target, the ICTS sensor had a linear range from 0.25 to 5 μg/L, with a correlation coefficient of 0.9901 and a detection limit of 0.1 μg/L. Furthermore, the repeatability of the ICTS was good, and the coefficient of variation was 10%. The ICTS immunosensor allows the reliable detection of MC-LR in water, and has potential in simple, sensitive detection applications. Graphical Abstract A novel method was developed to detect MC-LR using QDs based immunochromatographic test strip.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu, 214122, China.
authors.forename: Jiadi
authors.initials: J
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu, 214122, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu, 214122, China.
authors.forename: Fuwei
authors.initials: F
authors.lastname: Pi
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu, 214122, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Ji
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu, 214122, China.
authors.forename: Yinzhi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu, 214122, China. sxlzzz@jiangnan.edu.cn.
authors.forename: Xiulan
authors.initials: X
authors.lastname: Sun
mesh_terms: D002846:Chromatography, Affinity / Q000295:instrumentation*; D004591:Electrophoresis, Polyacrylamide Gel; D005453:Fluorescence; D057230:Limit of Detection; D008387:Marine Toxins; D052998:Microcystins / Q000032:analysis*; D045663:Quantum Dots; D015203:Reproducibility of Results
chemical_list: D008387:Marine Toxins; D052998:Microcystins; C057862:cyanoginosin LR
keywords: Immunochromatographic test strips; Microcystin-LR; Quantum dots