title: Systemic and mucosal pre-administration of recombinant Helicobacter pylori neutrophil-activating protein prevents ovalbumin-induced allergic asthma in mice.
abstract: Previous epidemiologic studies have demonstrated an inverse association between Helicobacter pylori infection and the frequency of allergic asthma. The neutrophil-activating protein (NAP) of H. pylori has been identified as a modulator possessing anti-Th2 inflammation activity. Here, we sought to determine whether systemic or mucosal pre-administration of recombinant H. pylori NAP (rNAP) could prevent ovalbumin (OVA)-induced allergic asthma in mice.
  
  
  
  Mice were exposed to purified rNAP through intraperitoneal injection or inhalation and then sensitized with OVA. Following a challenge with aerosolized OVA, the bronchoalveolar lavage fluid (BALF) cell count, lung tissue histology, BALF cytokines and serum IgE were evaluated.
  
  
  
  Both intraperitoneal injection and inhalation of rNAP prior to OVA sensitization significantly reduced eosinophil accumulation and inflammatory infiltration in lung tissue in OVA-induced asthma mice; eosinophils were reduced in the BALF of rNAP-treated mice. In addition, IL-4 and IL-13 levels were lower (P < 0.01), IL-10 and IFN-γ levels were higher (P < 0.01) and IgE serum levels were lower (P < 0.01) in the treated groups compared to the control group.
  
  
  
  Systemic and mucosal pre-administration of rNAP could suppress the development of OVA-induced asthma in mice; rNAP may be utilized as part of novel strategies for the prevention or treatment of allergic diseases.
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No. 318 Renminzhong Road, Yuexiu, Guangzhou, Guangdong 510120, People's Republic of China.|Translational Medicine Center, Guangdong Women and Children Hospital, No. 521 Xingnan Avenue, Panyu district, Guangzhou, Guangdong 511400, People's Republic of China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No. 318 Renminzhong Road, Yuexiu, Guangzhou, Guangdong 510120, People's Republic of China.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No. 318 Renminzhong Road, Yuexiu, Guangzhou, Guangdong 510120, People's Republic of China.
authors.forename: Bingshao
authors.initials: B
authors.lastname: Liang
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No. 318 Renminzhong Road, Yuexiu, Guangzhou, Guangdong 510120, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Dong
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No. 318 Renminzhong Road, Yuexiu, Guangzhou, Guangdong 510120, People's Republic of China.
authors.forename: Shuwen
authors.initials: S
authors.lastname: Yao
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No. 318 Renminzhong Road, Yuexiu, Guangzhou, Guangdong 510120, People's Republic of China.
authors.forename: Yinshuang
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No. 318 Renminzhong Road, Yuexiu, Guangzhou, Guangdong 510120, People's Republic of China.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No. 318 Renminzhong Road, Yuexiu, Guangzhou, Guangdong 510120, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Long
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No. 318 Renminzhong Road, Yuexiu, Guangzhou, Guangdong 510120, People's Republic of China.
authors.forename: Sitang
authors.initials: S
authors.lastname: Gong
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No. 318 Renminzhong Road, Yuexiu, Guangzhou, Guangdong 510120, People's Republic of China zzw6248@126.com.
authors.forename: Zhenwen
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D001249:Asthma / Q000139:chemically induced* / Q000517:prevention & control*; D001426:Bacterial Proteins / Q000008:administration & dosage*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D002648:Child; D016207:Cytokines / Q000032:analysis; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000517:prevention & control*; D007155:Immunologic Factors / Q000008:administration & dosage; D007274:Injections, Intraperitoneal; D007958:Leukocyte Count; D008168:Lung / Q000473:pathology; D008297:Male; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage* / Q000009:adverse effects*; D011994:Recombinant Proteins / Q000008:administration & dosage; D016896:Treatment Outcome
chemical_list: D001426:Bacterial Proteins; D016207:Cytokines; D007155:Immunologic Factors; D011994:Recombinant Proteins; C092412:neutrophil-activating protein A, Helicobacter pylori; D010047:Ovalbumin
keywords: Helicobacter pylori; allergic asthma; cytokines; inflammation; neutrophil-activating protein