title: Novel CagA ELISA exhibits enhanced sensitivity ofHelicobacter pyloriCagA antibody.
abstract: OBJECTIVE
  To develop a novelHelicobacter pylori(H. pylori) CagA antibody enzyme-linked immunosorbent assay (ELISA) suitable for detecting serum anti-CagA antibodies with high sensitivity.
  
  
  METHODS
  Recombinant East Asian-type CagA protein was purified and immobilized for ELISA. Serum samples from 217 Vietnamese individuals (110H. pylori-infected and 107 uninfected individuals) were applied. Conventional ELISA from Western-type CagA and our East Asian-type CagA ELISA were evaluated by comparing 38 subjects with the Western-type genotype and 72 subjects with the East Asian-typecagAgenotype. Histological scores of the gastric mucosa were determined using the updated Sydney System to examine the relationship with anti-CagA antibody titers.
  
  
  RESULTS
  Recombinant 70-100 kDa fragments were immobilized on the ELISA plate. In ROC analysis, the area under the curve of our East Asian-type CagA ELISA was comparable to that of conventional CagA ELISA. The sensitivity of the two ELISAs differed depending on thecagAgenotype. The sensitivity of East Asian-type CagA ELISA was higher for subjects infected with East Asian-typecagA H. pylori(P< 0.001), and the sensitivity of the conventional CagA ELISA tended to be higher for subjects infected with WesterncagA H. pylori(P= 0.056). The titer of anti-CagA antibody tended to correlate with monocyte infiltration scores (r= 0.25,P= 0.058) and was inversely correlated withH. pyloridensity (r= -0.26,P= 0.043).
  
  
  CONCLUSIONS
  The novel ELISA is useful to detect anti-CagA antibodies in East Asian countries, and the titer may be a marker for predicting chronic gastritis.
authors.affiliation: Yuichi Matsuo, Yasutoshi Kido, Junko Akada, Seiji Shiota, Tran Thanh Binh, Tran Thi Huyen Trang, Yoshio Yamaoka, Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, Oita 879-5593, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Matsuo
authors.affiliation: Yuichi Matsuo, Yasutoshi Kido, Junko Akada, Seiji Shiota, Tran Thanh Binh, Tran Thi Huyen Trang, Yoshio Yamaoka, Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, Oita 879-5593, Japan.
authors.forename: Yasutoshi
authors.initials: Y
authors.lastname: Kido
authors.affiliation: Yuichi Matsuo, Yasutoshi Kido, Junko Akada, Seiji Shiota, Tran Thanh Binh, Tran Thi Huyen Trang, Yoshio Yamaoka, Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, Oita 879-5593, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Akada
authors.affiliation: Yuichi Matsuo, Yasutoshi Kido, Junko Akada, Seiji Shiota, Tran Thanh Binh, Tran Thi Huyen Trang, Yoshio Yamaoka, Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, Oita 879-5593, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Shiota
authors.affiliation: Yuichi Matsuo, Yasutoshi Kido, Junko Akada, Seiji Shiota, Tran Thanh Binh, Tran Thi Huyen Trang, Yoshio Yamaoka, Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, Oita 879-5593, Japan.
authors.forename: Tran Thanh
authors.initials: TT
authors.lastname: Binh
authors.affiliation: Yuichi Matsuo, Yasutoshi Kido, Junko Akada, Seiji Shiota, Tran Thanh Binh, Tran Thi Huyen Trang, Yoshio Yamaoka, Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, Oita 879-5593, Japan.
authors.forename: Tran Thi Huyen
authors.initials: TT
authors.lastname: Trang
authors.affiliation: Yuichi Matsuo, Yasutoshi Kido, Junko Akada, Seiji Shiota, Tran Thanh Binh, Tran Thi Huyen Trang, Yoshio Yamaoka, Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, Oita 879-5593, Japan.
authors.forename: Ho D Q
authors.initials: HD
authors.lastname: Dung
authors.affiliation: Yuichi Matsuo, Yasutoshi Kido, Junko Akada, Seiji Shiota, Tran Thanh Binh, Tran Thi Huyen Trang, Yoshio Yamaoka, Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, Oita 879-5593, Japan.
authors.forename: Pham Huu
authors.initials: PH
authors.lastname: Tung
authors.affiliation: Yuichi Matsuo, Yasutoshi Kido, Junko Akada, Seiji Shiota, Tran Thanh Binh, Tran Thi Huyen Trang, Yoshio Yamaoka, Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, Oita 879-5593, Japan.
authors.forename: Tran Dinh
authors.initials: TD
authors.lastname: Tri
authors.affiliation: Yuichi Matsuo, Yasutoshi Kido, Junko Akada, Seiji Shiota, Tran Thanh Binh, Tran Thi Huyen Trang, Yoshio Yamaoka, Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, Oita 879-5593, Japan.
authors.forename: Ngo P Minh
authors.initials: NP
authors.lastname: Thuan
authors.affiliation: Yuichi Matsuo, Yasutoshi Kido, Junko Akada, Seiji Shiota, Tran Thanh Binh, Tran Thi Huyen Trang, Yoshio Yamaoka, Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, Oita 879-5593, Japan.
authors.forename: Le Quang
authors.initials: LQ
authors.lastname: Tam
authors.affiliation: Yuichi Matsuo, Yasutoshi Kido, Junko Akada, Seiji Shiota, Tran Thanh Binh, Tran Thi Huyen Trang, Yoshio Yamaoka, Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, Oita 879-5593, Japan.
authors.forename: Bui Chi
authors.initials: BC
authors.lastname: Nam
authors.affiliation: Yuichi Matsuo, Yasutoshi Kido, Junko Akada, Seiji Shiota, Tran Thanh Binh, Tran Thi Huyen Trang, Yoshio Yamaoka, Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, Oita 879-5593, Japan.
authors.forename: Vu Van
authors.initials: VV
authors.lastname: Khien
authors.affiliation: Yuichi Matsuo, Yasutoshi Kido, Junko Akada, Seiji Shiota, Tran Thanh Binh, Tran Thi Huyen Trang, Yoshio Yamaoka, Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, Oita 879-5593, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Yamaoka
mesh_terms: D000942:Antigens, Bacterial / Q000097:blood / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000097:blood / Q000235:genetics / Q000276:immunology*; D001706:Biopsy; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay; D005753:Gastric Mucosa / Q000382:microbiology* / Q000473:pathology; D005756:Gastritis / Q000097:blood / Q000175:diagnosis* / Q000382:microbiology; D005773:Gastroscopy; D005838:Genotype; D016481:Helicobacter Infections / Q000097:blood / Q000175:diagnosis* / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity; D014744:Vietnam
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D011994:Recombinant Proteins; C080866:cagA protein, Helicobacter pylori
keywords: Anti-CagA antibody; Enzyme-linked immunosorbent assay; Gastritis; Helicobacter pylori; Inflammation; cagA genotype