title: Effects of umbilical cord blood stem cells on healing factors for diabetic foot injuries.
abstract: The use of stem or progenitor cells from bone marrow, or peripheral or umbilical cord blood is becoming more common for treatment of diabetic foot problems. These cells promote neovascularization by angiogenic factors and they promote epithelium formation by stimulating cell replication and migration under certain pathological conditions. We investigated the role of CD34 + stem cells from human umbilical cord blood in wound healing using a rat model. Rats were randomly divided into a control group and two groups with diabetes induced by a single dose of 55 mg/kg intraperitoneal streptozocin. Scarred areas 5 mm in diameter were created on the feet of all rats. The diabetic rats constituted the diabetes control group and a diabetes + stem cell group with local injection into the wound site of 0.5 × 106 CD34 + stem cells from human umbilical cord blood. The newly formed skin in the foot wounds following CD34 + stem cell treatment showed significantly improvement by immunohistochemistry and TUNEL staining, and were closer to the wound healing of the control group than the untreated diabetic animals. The increase in FGF expression that accompanied the local injection of CD34 + stem cells indicates that FGF stimulation helped prevent apoptosis. Our findings suggest a promising new treatment approach to diabetic wound healing.
authors.affiliation: a Departments of Histology and Embryology.
authors.forename: N
authors.initials: N
authors.lastname: Çil
authors.affiliation: a Departments of Histology and Embryology.
authors.forename: E O
authors.initials: EO
authors.lastname: Oğuz
authors.affiliation: b Departments of Microbiology, Faculty of Medicine , Pamukkale University , Denizli , Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Mete
authors.affiliation: a Departments of Histology and Embryology.
authors.forename: A
authors.initials: A
authors.lastname: Çetinkaya
authors.affiliation: a Departments of Histology and Embryology.
authors.forename: G A
authors.initials: GA
authors.lastname: Mete
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000008:administration & dosage / Q000494:pharmacology*; D015415:Biomarkers; D003921:Diabetes Mellitus, Experimental; D017719:Diabetic Foot / Q000188:drug therapy*; D005312:Fetal Blood / Q000166:cytology*; D005786:Gene Expression Regulation; D006801:Humans; D018919:Neovascularization, Physiologic; D011897:Random Allocation; D051381:Rats; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000502:physiology*; D014945:Wound Healing
chemical_list: D018952:Antigens, CD34; D015415:Biomarkers
keywords: CD34; Caspase 3; FGF; IL-1; PDGF; TGF-α; TNF-β; apoptosis VEGF; diabetic foot; wound healing