title: Identification of a novel protein kinase that affects the chronological lifespan in fission yeast.
abstract: Chronological lifespan is defined by how long a cell can survive in a non-dividing state. In yeast, it is measured by viability after entry into the stationary phase. To understand the regulatory mechanisms of chronological lifespan in Schizosaccharomyces pombe, it is necessary to identify and characterize novel factors involved in the regulation of chronological lifespan. To this end, we have screened for a long-lived mutant and identified that novel gene nnk1+that encodes an essential protein kinase is the determinant of chronological lifespan. We showed that the expression of major glucose transporter gene, ght5+, is decreased in the isolated nnk1-35 mutant, suggesting that Nnk1 protein is involved in the regulation of ght5+The consumption of glucose in the growth medium after saturated growth was lower in the nnk1-35 mutant than that in wild-type cell. The isolated ght5 deletion mutant showed long-lived phenotype. Based on these results, we propose that Nnk1 regulates chronological lifespan through the regulation of ght5+Nnk1 might coordinate glucose availability and lifespan in fission yeast.
authors.affiliation: Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.
authors.forename: Tatsuhiro
authors.initials: T
authors.lastname: Kurauchi
authors.affiliation: Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.
authors.forename: Aya
authors.initials: A
authors.lastname: Hashizume
authors.affiliation: Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Imai
authors.affiliation: Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.
authors.forename: Kanako
authors.initials: K
authors.lastname: Hayashi
authors.affiliation: Laboratory of Molecular Microbiology, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tsubouchi
authors.affiliation: Center for Gene Research, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.
authors.forename: Kunio
authors.initials: K
authors.lastname: Ihara
authors.affiliation: Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.
authors.forename: Hokuto
authors.initials: H
authors.lastname: Ohtsuka
authors.affiliation: Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan aiba@ps.nagoya-u.ac.jp.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Aiba
mesh_terms: D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D015870:Gene Expression; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism; D050296:Microbial Viability; D009004:Monosaccharide Transport Proteins / Q000096:biosynthesis; D009154:Mutation; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000502:physiology*; D029702:Schizosaccharomyces pombe Proteins / Q000096:biosynthesis
chemical_list: D005656:Fungal Proteins; C000601636:Ght5 protein, S pombe; D009004:Monosaccharide Transport Proteins; D029702:Schizosaccharomyces pombe Proteins; D011494:Protein Kinases; D005947:Glucose
keywords: Lifespan; Nnk1; Schizosaccharomyces pombe; aging, glucose transporter; yeast