title: Effects of local vanadium delivery on diabetic fracture healing.
abstract: This study evaluated the effect of local vanadyl acetylacetonate (VAC), an insulin mimetic agent, upon the early and late parameters of fracture healing in rats using a standard femur fracture model. Mechanical testing, and radiographic scoring were performed, as well as histomorphometry, including percent bone, percent cartilage, and osteoclast numbers. Fractures treated with local 1.5 mg/kg VAC possessed significantly increased mechanical properties compared to controls at 6 weeks post-fracture, including increased torque to failure (15%; p = 0.046), shear modulus (89%; p = 0.043), and shear stress (81%; p = 0.009). The radiographic scoring analysis showed increased cortical bridging at 4 weeks and 6 weeks (119%; p = 0.036 and 209%; p = 0.002) in 1.5 mg/kg VAC treated groups. Histomorphometry of the fracture callus at days 10 and 14 showed increased percent cartilage (121%; p = 0.009 and 45%; p = 0.035) and percent mineralized tissue (66%; p = 0.035 and 58%; p = 0.006) with local VAC treated groups compared to control. Additionally, fewer osteoclasts were observed in the local VAC treated animals as compared to controls at day 14 (0.45% ± 0.29% vs. 0.83% ± 0.36% of callus area; p = 0.032). The results suggest local administration of VAC acts to modulate osteoclast activity and increase percentage of early callus cartilage, ultimately enhancing mechanical properties comparably to non-diabetic animals treated with local VAC. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:2174-2180, 2017.
authors.affiliation: Department of Orthopaedics, Rutgers New Jersey Medical School, 90 Bergen Street, Suite 7300, Newark, New Jersey 07101.
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Ippolito
authors.affiliation: Department of Orthopaedics, Rutgers New Jersey Medical School, 90 Bergen Street, Suite 7300, Newark, New Jersey 07101.
authors.forename: Ethan S
authors.initials: ES
authors.lastname: Krell
authors.affiliation: Department of Biological Sciences, Seton Hall University, South Orange, New Jersey.
authors.forename: Jessica
authors.initials: J
authors.lastname: Cottrell
authors.affiliation: Department of Orthopaedics, Rutgers New Jersey Medical School, 90 Bergen Street, Suite 7300, Newark, New Jersey 07101.
authors.forename: Ryan
authors.initials: R
authors.lastname: Meyer
authors.affiliation: Department of Orthopaedics, Rutgers New Jersey Medical School, 90 Bergen Street, Suite 7300, Newark, New Jersey 07101.
authors.forename: Devin
authors.initials: D
authors.lastname: Clark
authors.affiliation: Department of Orthopaedics, Rutgers New Jersey Medical School, 90 Bergen Street, Suite 7300, Newark, New Jersey 07101.
authors.forename: Daniel
authors.initials: D
authors.lastname: Nguyen
authors.affiliation: Department of Orthopaedics, Rutgers New Jersey Medical School, 90 Bergen Street, Suite 7300, Newark, New Jersey 07101.
authors.forename: Suleiman
authors.initials: S
authors.lastname: Sudah
authors.affiliation: Department of Orthopaedics, Rutgers New Jersey Medical School, 90 Bergen Street, Suite 7300, Newark, New Jersey 07101.
authors.forename: Maximillian
authors.initials: M
authors.lastname: Muñoz
authors.affiliation: Department of Orthopaedics, Rutgers New Jersey Medical School, 90 Bergen Street, Suite 7300, Newark, New Jersey 07101.
authors.forename: Elisha
authors.initials: E
authors.lastname: Lim
authors.affiliation: Department of Orthopaedics, Rutgers New Jersey Medical School, 90 Bergen Street, Suite 7300, Newark, New Jersey 07101.
authors.forename: Anthony
authors.initials: A
authors.lastname: Lin
authors.affiliation: Department of Orthopaedics, Rutgers New Jersey Medical School, 90 Bergen Street, Suite 7300, Newark, New Jersey 07101.
authors.forename: Thomas Jae Hoon
authors.initials: TJH
authors.lastname: Lee
authors.affiliation: Department of Orthopaedics, Rutgers New Jersey Medical School, 90 Bergen Street, Suite 7300, Newark, New Jersey 07101.
authors.forename: James Patrick
authors.initials: JP
authors.lastname: O'Connor
authors.affiliation: Department of Orthopaedics, Rutgers New Jersey Medical School, 90 Bergen Street, Suite 7300, Newark, New Jersey 07101.
authors.forename: Joseph
authors.initials: J
authors.lastname: Benevenia
authors.affiliation: Department of Orthopaedics, Rutgers New Jersey Medical School, 90 Bergen Street, Suite 7300, Newark, New Jersey 07101.
authors.forename: Sheldon S
authors.initials: SS
authors.lastname: Lin
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D004353:Drug Evaluation, Preclinical; D005264:Femoral Fractures / Q000150:complications / Q000000981:diagnostic imaging / Q000188:drug therapy*; D017102:Fracture Healing / Q000187:drug effects*; D011859:Radiography; D017208:Rats, Wistar; D014131:Trace Elements / Q000008:administration & dosage*; D014639:Vanadium / Q000008:administration & dosage*
chemical_list: D014131:Trace Elements; D014639:Vanadium
keywords: BB Wistar; bone; cartilage; diabetes; fracture healing; insulin mimetic fracture healing; mechanical strength; vanadium