title: Phytochemical study and protective effect of Trigonella foenum graecum (Fenugreek seeds) against carbon tetrachloride-induced toxicity in liver and kidney of male rat.
abstract: Liver and kidney diseases are a global concern, therefore considerable efforts to obtain fine herbs useful as drugs from medicinal plants are currently in progress. The aim of this work was to study the antioxidant effects of previous supplementation with fenugreek seeds (FS) against carbon tetrachloride (CCl4) toxicity in the liver and kidney. CCl4toxicity was induced by one dose (i.g. 5ml CCl4/kg of body weight, 50% CCl4in olive oil) after 7 weeks of normal diet or diet rich in 10% of grinded fenugreek seeds (20g of pellet rat food/rat/day). 24h after the treatment with CCl4, all animals were scarified and biological analyses were performed. A phytochemical study of fenugreek seed extract (FSE) was also carried out. The phytochemical analysis of FS and FSE revealed the presence of polyphenols (5.92±0.02mg EGA/g DM), flavonoids (0.44±0.19mg ER/g DM), polysaccharides and trace elements. DPPH radical-scavenging activity of FSE showed an EC50 of 285.59±2.01μg/ml. In vivo, CCl4administration significantly (p<0.05) induced an increase liver and kidney biomarkers. A significant (p<0.05) alteration of the antioxidant enzyme activities was also observed. In animals pretreated with FS, the studied parameters were much less shifted. These results indicate that the supplementation with fenugreek seeds is significantly effective in protecting the liver and kidneys from CCl4toxicity.
authors.affiliation: Laboratory of Environmental pathophysiology, Development of bioactive molecules and mathematical modeling, Faculty of Science, 3018 Sfax, Tunisia. Electronic address: mbarki_sakhria@yahoo.com.
authors.forename: Sakhria
authors.initials: S
authors.lastname: Mbarki
authors.affiliation: Research unit of Macromolecular Biochemistry and Genetics, Faculty of Sciencces of Gafsa, 2112 Gafsa, Tunisia.
authors.forename: Hichem
authors.initials: H
authors.lastname: Alimi
authors.affiliation: Laboratory of Environmental pathophysiology, Development of bioactive molecules and mathematical modeling, Faculty of Science, 3018 Sfax, Tunisia.
authors.forename: Hafsia
authors.initials: H
authors.lastname: Bouzenna
authors.affiliation: Laboratory of Environmental pathophysiology, Development of bioactive molecules and mathematical modeling, Faculty of Science, 3018 Sfax, Tunisia.
authors.forename: Abdelfettah
authors.initials: A
authors.lastname: Elfeki
authors.affiliation: Laboratory of Environmental pathophysiology, Development of bioactive molecules and mathematical modeling, Faculty of Science, 3018 Sfax, Tunisia.
authors.forename: Najla
authors.initials: N
authors.lastname: Hfaiedh
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002251:Carbon Tetrachloride / Q000633:toxicity*; D005419:Flavonoids / Q000032:analysis; D016166:Free Radical Scavengers / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects / Q000473:pathology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000473:pathology*; D008297:Male; D010636:Phenols / Q000032:analysis; D064209:Phytochemicals / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D020011:Protective Agents / Q000494:pharmacology*; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014020:Tissue Extracts; D029910:Trigonella / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D016166:Free Radical Scavengers; D010636:Phenols; D064209:Phytochemicals; D010936:Plant Extracts; D020011:Protective Agents; D017392:Thiobarbituric Acid Reactive Substances; D014020:Tissue Extracts; D002251:Carbon Tetrachloride
keywords: Antioxidant compounds; CCl(4); Kidney; Liver; Oxidative stress; Trigonella