title: Using Reciprocal Protein-Peptide Array Screening to Unravel Protein Interaction Networks.
abstract: Protein-protein interactions (PPIs) play a central role in almost all cellular processes. Recent technological advances have enabled the elucidation of an incredibly complex PPI network within the cell. However, protein interactions driven by posttranslational modifications (PTMs) such as phosphorylation, which comprises a significant part of the PPI network, have proven difficult to decipher systematically. Herein, we describe a reciprocal protein-peptide array strategy to uncover PPIs mediated by tyrosine phosphorylation and the Src homology 2 (SH2) domain. This strategy, namely combining peptide and protein domain arrays for PPI mapping, may be applicable for other peptide-binding modules.
authors.affiliation: Department of Biochemistry, Siebens-Drake Medical Research Institute, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, N6A 5C1, Canada.
authors.forename: Huadong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Biochemistry, Siebens-Drake Medical Research Institute, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, N6A 5C1, Canada.
authors.forename: Courtney
authors.initials: C
authors.lastname: Voss
authors.affiliation: Department of Biochemistry, Siebens-Drake Medical Research Institute, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, N6A 5C1, Canada. sli@uwo.ca.
authors.forename: Shawn S C
authors.initials: SS
authors.lastname: Li
mesh_terms: D019295:Computational Biology / Q000379:methods; D006801:Humans; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D010748:Phosphopeptides; D010766:Phosphorylation; D040081:Protein Array Analysis / Q000379:methods*; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping / Q000379:methods*; D060066:Protein Interaction Maps; D011993:Recombinant Fusion Proteins; D018909:src Homology Domains
chemical_list: D010455:Peptides; D010748:Phosphopeptides; D011993:Recombinant Fusion Proteins
keywords: Peptide array; Phosphorylation; Protein array; Protein-protein interaction; SH2 domain