title: Rapid Aminoglycoside NP Test for Rapid Detection of Multiple Aminoglycoside Resistance in Enterobacteriaceae.
abstract: The rapid aminoglycoside NP (Nordmann/Poirel) test was developed to rapidly identify multiple aminoglycoside (AG) resistance inEnterobacteriaceaeIt is based on the detection of the glucose metabolism related to enterobacterial growth in the presence of a defined concentration of amikacin plus gentamicin. Formation of acid metabolites was evidenced by a color change (orange to yellow) of the red phenol pH indicator. The rapid aminoglycoside NP test was evaluated by using bacterial colonies of 18 AG-resistant isolates producing 16S rRNA methylases, 20 AG-resistant isolates expressing AG-modifying enzymes (acetyl-, adenyl-, and phosphotransferases), and 10 isolates susceptible to AG. Its sensitivity and specificity were 100% and 97%, respectively, compared to the broth dilution method, which was taken as the gold standard for determining aminoglycoside resistance. The test is inexpensive, rapid (<2 h), and implementable worldwide.
authors.affiliation: Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, INSERM European Unit, LEA, IAME, Paris, France, and Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland patrice.nordmann@unifr.ch.|Institute of Microbiology, University Hospital Centre and University of Lausanne, Lausanne, Switzerland.
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
authors.affiliation: Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, INSERM European Unit, LEA, IAME, Paris, France, and Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland.|Laboratoire de Bactériologie, CHU de Bordeaux, and CNRS UMR5234 Microbiologie Fondamentale et Pathogénicité, University of Bordeaux, Bordeaux, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Jayol
authors.affiliation: Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, INSERM European Unit, LEA, IAME, Paris, France, and Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland.
authors.forename: Jan
authors.initials: J
authors.lastname: Dobias
authors.affiliation: Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, INSERM European Unit, LEA, IAME, Paris, France, and Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland.
authors.forename: Laurent
authors.initials: L
authors.lastname: Poirel
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003365:Costs and Cost Analysis; D024881:Drug Resistance, Bacterial; D004755:Enterobacteriaceae / Q000187:drug effects*; D008826:Microbial Sensitivity Tests / Q000191:economics / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents
keywords: 16S rRNA methylases; amikacin; antibiotic; gentamicin; kanamycin; netilmicin; plazomicin; rapid diagnostic test; susceptibility testing; tobramycin