title: A development and an improvement of selectable markers in Pleurotus ostreatus transformation.
abstract: Pleurotus ostreatus was transformed using the nourseothricin-resistant gene for the first time. The transformation efficiency was 1.3±0.6transformants/μg plasmid DNA. In addition, the transformation efficiency of the bialaphos-resistant gene was increased to 26.7±11.5transformants/μg plasmid DNA.
authors.affiliation: School of Environmental Science, University of Shiga Prefecture, 2500 Hassaka-cho, Hikone, Shiga 522-8533, Japan.
authors.forename: Yukari
authors.initials: Y
authors.lastname: Matsunaga
authors.affiliation: School of Environmental Science, University of Shiga Prefecture, 2500 Hassaka-cho, Hikone, Shiga 522-8533, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Ando
authors.affiliation: School of Environmental Science, University of Shiga Prefecture, 2500 Hassaka-cho, Hikone, Shiga 522-8533, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Izumitsu
authors.affiliation: School of Environmental Science, University of Shiga Prefecture, 2500 Hassaka-cho, Hikone, Shiga 522-8533, Japan.
authors.forename: Kazumi
authors.initials: K
authors.lastname: Suzuki
authors.affiliation: Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto Prefecture 606-8502, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Honda
authors.affiliation: School of Environmental Science, University of Shiga Prefecture, 2500 Hassaka-cho, Hikone, Shiga 522-8533, Japan. Electronic address: tirie@ses.usp.ac.jp.
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Irie
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D004271:DNA, Fungal / Q000235:genetics*; D025141:Drug Resistance, Fungal / Q000235:genetics; D005819:Genetic Markers; D009943:Organophosphorus Compounds / Q000494:pharmacology; D010957:Plasmids; D020076:Pleurotus / Q000187:drug effects / Q000235:genetics*; D013309:Streptothricins / Q000494:pharmacology; D014170:Transformation, Genetic
chemical_list: D000935:Antifungal Agents; D004271:DNA, Fungal; D005819:Genetic Markers; D009943:Organophosphorus Compounds; C003122:bialaphos; D013309:Streptothricins
keywords: Basidiomycete; Mushroom; White-rot