title: Molecular Characterization ofNippostrongylus brasiliensis(Nematoda: Heligmosomatidae) fromMus musculusin India.
abstract: Mus musculus(Rodentia: Muridae) has generally been infected with a rodent hookwormNippostrongylus brasiliensis. In this report, we present morphological and molecular identification ofN. brasiliensisby light and scanning electron microscopy and PCR amplification of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene and the protein sequences encoded bycox1gene, respectively. Despite the use ofN. brasiliensisin many biochemistry studies from India, their taxonomic identification was not fully understood, especially at the species level, and no molecular data is available in GenBank from India. Sequence analysis ofcox1gene in this study revealed that the present specimen showed close identity with the same species available in GenBank, confirming that the species isN. brasiliensis. This study represents the first record of molecular identification ofN. brasiliensisfrom India and the protein structure to better understand the comparative phylogenetic characteristics.
authors.affiliation: Molecular Taxonomy Laboratory, Department of Zoology, Chaudhary Charan Singh University, Meerut (U.P.), 250004, India.
authors.forename: Anshu
authors.initials: A
authors.lastname: Chaudhary
authors.affiliation: Molecular Taxonomy Laboratory, Department of Zoology, Chaudhary Charan Singh University, Meerut (U.P.), 250004, India.
authors.forename: Urvashi
authors.initials: U
authors.lastname: Goswami
authors.affiliation: Molecular Taxonomy Laboratory, Department of Zoology, Chaudhary Charan Singh University, Meerut (U.P.), 250004, India.
authors.forename: Hridaya Shanker
authors.initials: HS
authors.lastname: Singh
mesh_terms: D000825:Animal Structures / Q000033:anatomy & histology; D000818:Animals; D016000:Cluster Analysis; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D005260:Female; D007194:India; D008297:Male; D051379:Mice; D008853:Microscopy; D009559:Nippostrongylus / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D012376:Rodent Diseases / Q000469:parasitology*; D017422:Sequence Analysis, DNA; D017206:Strongylida Infections / Q000469:parasitology / Q000662:veterinary*
chemical_list: D018509:DNA, Helminth; D003576:Electron Transport Complex IV
keywords: Meerut; Mus musculus; Nippostrongylus brasiliensis; Uttar Pradesh; mitochondrial cox1; nematode; protein