title: Inhibition of Apoptosis Stages of Human Blood Lymphocytes after Exposure to Carbon Monoxide in the Presence of Recombinant Interleukin-2.
abstract: We studied the effect of carbon monoxide (60-, 75-, and 90-min exposure) on the expression of antiapoptotic proteins (survivin and Bcl-2) in human blood lymphocytes in the presence of recombinant IL-2 in an apoptosis-inducing dose (0.1 ng/ml). Incubation of cells in atmosphere with carbon monoxide in the presence of recombinant IL-2 was accompanied by accumulation of Bcl-2 protein with simultaneous decrease of survivin content. It was concluded that carbon monoxide plays a role in the dysregulation of apoptosis of human blood lymphocytes Bcl-2 (i.e. CO inhibits the proapoptotic effect of recombinant IL-2).
authors.affiliation: Voronezh State University, Voronezh, Russia. artyukhov@bio.vsu.ru.
authors.forename: V G
authors.initials: VG
authors.lastname: Artyukhov
authors.affiliation: N. N. Burdenko Voronezh State Medical University, Voronezh, Russia.
authors.forename: O I
authors.initials: OI
authors.lastname: Tyunina
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D002248:Carbon Monoxide / Q000494:pharmacology*; D005786:Gene Expression Regulation; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D007376:Interleukin-2 / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D061251:Primary Cell Culture; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000819:agonists / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction; D000077022:Survivin
chemical_list: C000595707:BCL2 protein, human; C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D007376:Interleukin-2; D019253:Proto-Oncogene Proteins c-bcl-2; D011994:Recombinant Proteins; D000077022:Survivin; D002248:Carbon Monoxide
keywords: Bcl-2; carbon monoxide; lymphocytes; recombinant interleukin-2; survivin