title: Profile of Inflammation-Associated Proteins in Early Post-Transplant Samples of Patients After Allogeneic Hematopoietic Stem Cell Transplantation: a Preliminary Study.
abstract: Allogeneic hematopoietic stem cell transplantation (aHSCT) is used as a curative treatment in severe hematological and immunological disorders. Despite clear improvement of the aHSCT outcome, substantial proportion of patients still suffers from severe complications, including graft-versus-host disease (GvHD). The aim of this study was, therefore, to identify inflammation-associated molecules deregulated in the early serum samples of the patients after aHSCT and nominate markers associated with particular aHSCT parameters/complications. Serum concentrations of 92 inflammation-associated proteins were measured in samples obtained from 80 aHSCT patients 14 days after transplantation and from 23 healthy control subjects by a novel sensitive proximity extension assay technology using Proseek Multiplex Inflammation I kit. Serum profiles of inflammatory proteins in patients after aHSCT were substantially different from those observed in control subjects and related to underlying disease status before transplantation. Particularly, the difference between aHSCT patients and controls reached significance level for 57 analytes (40 upregulated, 17 downregulated in aHSCT patients). The concentration of several markers was associated with the level of donor/recipient HLA match (TGF-α: pcorr= 0.025, HGF: pcorr= 0.036) and with complete donor chimerism at day +30 after allografting (DNER: pcorr= 0.042). None of the markers was significantly associated with acute and chronic GvHD after correction. More than half of investigated proteins significantly differed between the samples from aHSCT patients and healthy control subjects as a consequence of the "cytokine storm" after aHSCT. Comparisons of patient's subgroups based on specific biological/clinical parameters revealed much less evident differences; nevertheless, we nominated several markers associated with the level of donor/recipient HLA match and post-transplant chimerism.
authors.affiliation: Department of Immunology, Faculty of Medicine and Dentistry, Palacky University and University Hospital, I. P. Pavlova 185/6, 779 00, Olomouc, Czech Republic. mrazekf@fnol.cz.
authors.forename: Frantisek
authors.identifier: http://orcid.org/0000-0002-9981-7517
authors.initials: F
authors.lastname: Mrazek
authors.affiliation: Department of Immunology, Faculty of Medicine and Dentistry, Palacky University and University Hospital, I. P. Pavlova 185/6, 779 00, Olomouc, Czech Republic.
authors.forename: Petra
authors.initials: P
authors.lastname: Schneiderova
authors.affiliation: Department of Immunology, Faculty of Medicine and Dentistry, Palacky University and University Hospital, I. P. Pavlova 185/6, 779 00, Olomouc, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Kriegova
authors.affiliation: Department of Haemato-Oncology, Faculty of Medicine and Dentistry, Palacky University and University Hospital, Olomouc, Czech Republic.
authors.forename: Ludek
authors.initials: L
authors.lastname: Raida
authors.affiliation: Department of Haemato-Oncology, Faculty of Medicine and Dentistry, Palacky University and University Hospital, Olomouc, Czech Republic.
authors.forename: Adam
authors.initials: A
authors.lastname: Kuba
authors.affiliation: Department of Computer Science, Faculty of Electrical Engineering and Computer Science, VSB-Technical University of Ostrava, Ostrava, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Gajdos
authors.affiliation: Department of Immunology, Faculty of Medicine and Dentistry, Palacky University and University Hospital, I. P. Pavlova 185/6, 779 00, Olomouc, Czech Republic.
authors.forename: Nikola
authors.initials: N
authors.lastname: Königova
authors.affiliation: Department of Immunology, Faculty of Medicine and Dentistry, Palacky University and University Hospital, I. P. Pavlova 185/6, 779 00, Olomouc, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Onderkova
authors.affiliation: Department of Immunology, Faculty of Medicine and Dentistry, Palacky University and University Hospital, I. P. Pavlova 185/6, 779 00, Olomouc, Czech Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Ambruzova
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis; D005260:Female; D006086:Graft vs Host Disease / Q000276:immunology; D019337:Hematologic Neoplasms / Q000628:therapy*; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects / Q000379:methods*; D006801:Humans; D007249:Inflammation / Q000097:blood* / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D011184:Postoperative Period; D019172:Transplantation Conditioning / Q000009:adverse effects / Q000379:methods*; D014184:Transplantation, Homologous; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D015415:Biomarkers; D001798:Blood Proteins
keywords: Biomarkers; Graft-versus-host disease; Hematopoetic stem cell transplantation; Inflammation; Protein profiles