title: Centromere Stability: The Replication Connection.
abstract: The fission yeast centromere, which is similar to metazoan centromeres, contains highly repetitive pericentromere sequences that are assembled into heterochromatin. This is required for the recruitment of cohesin and proper chromosome segregation. Surprisingly, the pericentromere replicates early in the S phase. Loss of heterochromatin causes this domain to become very sensitive to replication fork defects, leading to gross chromosome rearrangements. This review examines the interplay between components of DNA replication, heterochromatin assembly, and cohesin dynamics that ensures maintenance of genome stability and proper chromosome segregation.
authors.affiliation: Program in Molecular & Computational Biology, University of Southern California, Los Angeles, CA 90089-2910, USA. Forsburg@usc.edu.
authors.forename: Susan L
authors.initials: SL
authors.lastname: Forsburg
authors.affiliation: Program in Molecular & Computational Biology, University of Southern California, Los Angeles, CA 90089-2910, USA. kuofangs@usc.edu.
authors.forename: Kuo-Fang
authors.initials: KF
authors.lastname: Shen
keywords: Fork Protection Complex; Swi6; centromere; cohesion; fragile site; heterochromatin; replication