title: RNA-Seq transcriptome analysis of the olive flounder (Paralichthys olivaceus) kidney response to vaccination with heat-inactivated viral hemorrhagic septicemia virus.
abstract: Viral hemorrhagic septicemia (VHS) is a highly contagious disease of cultured flounder caused by VHS virus (VHSV). To develop effective VHSV vaccines, it is essential to understand the molecular mechanisms underlying the host's protective response against VHSV. The purpose of this study is to clarify which genes are involved in the protective response of olive flounder after VHSV vaccination. We first injected olive flounder intraperitoneally with 107TCID50heat-inactivated VHSV vaccine and evaluated the vaccine efficacy at 20 °C. Fish vaccinated with heat-inactivated VHSV were significantly protected compared to non-vaccinated fish, with a relative percentage survival of 83%. To analyze the vaccination-induced changes in the expression profiles of genes, kidneys were collected from control and vaccinated fish at days 1, 3, and 7 after vaccination and global gene expression profiling was carried out by RNA sequencing. The analysis revealed that 15,001 genes were differentially expressed by at least 2-fold between vaccinated fish and non-vaccinated controls. Of these, 58 genes clustered into the acute phase response, Toll-like receptor, interferon-inducible/regulatory proteins, and apoptosis pathways. These data provided insights into the molecular mechanisms underlying the protective immune response of olive flounder against heat-inactivated VHSV vaccine and might aid future studies to develop a highly immunogenic vaccine against VHSV in flounder.
authors.affiliation: Aquatic Disease Control Division, National Institute of Fisheries Science, 216 GijangHaean-Ro, Gijang-up, Gijang-Gun, Busan 46083, Republic of Korea. Electronic address: jinihwang@korea.kr.
authors.forename: Jee Youn
authors.initials: JY
authors.lastname: Hwang
authors.affiliation: Aquatic Disease Control Division, National Institute of Fisheries Science, 216 GijangHaean-Ro, Gijang-up, Gijang-Gun, Busan 46083, Republic of Korea. Electronic address: mgkwon@korea.kr.
authors.forename: Mun Gyeoung
authors.initials: MG
authors.lastname: Kwon
authors.affiliation: Pathology Division, National Institute of Fisheries Science, 216 GijangHaean-Ro, Gijang-up, Gijang-Gun, Busan 46083, Republic of Korea. Electronic address: immu@korea.kr.
authors.forename: Sung-Hee
authors.initials: SH
authors.lastname: Jung
authors.affiliation: Southeast Sea Fisheries Research Institute, National Institute of Fisheries Science (NIFS), Tongyeong 650-943, Republic of Korea. Electronic address: mgpark@korea.kr.
authors.forename: Myoung Ae
authors.initials: MA
authors.lastname: Park
authors.affiliation: AllBT Co., Ltd., O1-315 Ho, Internet Business Incubation Center, Mokweon University, 88 Doanbuk-ro, Seo-gu, Daejeon 302-729, Republic of Korea. Electronic address: kdw9017@naver.com.
authors.forename: Dong-Wook
authors.initials: DW
authors.lastname: Kim
authors.affiliation: AllBT Co., Ltd., O1-315 Ho, Internet Business Incubation Center, Mokweon University, 88 Doanbuk-ro, Seo-gu, Daejeon 302-729, Republic of Korea. Electronic address: marronnier@hanmail.net.
authors.forename: Wang Sik
authors.initials: WS
authors.lastname: Cho
authors.affiliation: Department of Biological Sciences, University of Ulsan, Ulsan 680-749, Republic of Korea. Electronic address: jwpark@ulsan.ac.kr.
authors.forename: Jeong Woo
authors.initials: JW
authors.lastname: Park
authors.affiliation: Aquatic Disease Control Division, National Institute of Fisheries Science, 216 GijangHaean-Ro, Gijang-up, Gijang-Gun, Busan 46083, Republic of Korea. Electronic address: hyu782@korea.kr.
authors.forename: Maeng-Hyun
authors.initials: MH
authors.lastname: Son
mesh_terms: D000818:Animals; D005393:Fish Diseases / Q000276:immunology / Q000517:prevention & control; D005412:Flatfishes; D020869:Gene Expression Profiling / Q000662:veterinary; D031941:Hemorrhagic Septicemia, Viral / Q000276:immunology / Q000517:prevention & control; D059014:High-Throughput Nucleotide Sequencing / Q000662:veterinary; D007274:Injections, Intraperitoneal / Q000662:veterinary; D029141:Novirhabdovirus / Q000276:immunology*; D059467:Transcriptome; D014611:Vaccination / Q000662:veterinary*; D015164:Vaccines, Inactivated / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D015164:Vaccines, Inactivated; D014765:Viral Vaccines
keywords: Heat-killed vaccine; Olive flounder; RNA-seq; Viral hemorrhagic septicemia virus