title: A report on identification of sequence polymorphism in barcode region of six commercially important Cymbopogon species.
abstract: CYMBOPOGON: is an important member of grass family Poaceae, cultivated for essential oils which have greater medicinal and industrial value. Taxonomic identification of Cymbopogon species is determined mainly by morphological markers, odour of essential oils and concentration of bioactive compounds present in the oil matrices which are highly influenced by environment. Authenticated molecular marker based taxonomical identification is also lacking in the genus; hence effort was made to evaluate potential DNA barcode loci in six commercially important Cymbopogon species for their individual discrimination and authentication at the species level. Four widely used DNA barcoding regions viz., ITS 1 & ITS 2 spacers, matK, psbA-trnH and rbcL were taken for the study. Gene sequences of the same or related genera of the concerned loci were mined from NCBI domain and primers were designed and validated for barcode loci amplification. Out of the four loci studied, sequences from matK and ITS spacer loci revealed 0.46% and 5.64% nucleotide sequence diversity, respectively whereas the other two loci i.e., psbA-trnH and rbcL showed 100% sequence homology. The newly developed primers can be used for barcode loci amplification in the genus Cymbopogon. The identified Single Nucleotide Polymorphisms from the studied sequences may be used as barcodes for the six Cymbopogon species. The information generated can also be utilized for barcode development of the genus by including more number of Cymbopgon species in future.
authors.affiliation: PDPIAS, Charotar University of Science and Technology, Changa, Anand, Gujarat, India.|ICAR-Directorate of Medicinal and Aromatic Plant Research, Boriavi, Anand, Gujarat, India.
authors.forename: Ashok Kumar
authors.initials: AK
authors.lastname: Bishoyi
authors.affiliation: ICAR-Directorate of Medicinal and Aromatic Plant Research, Boriavi, Anand, Gujarat, India.
authors.forename: Aarti
authors.initials: A
authors.lastname: Kavane
authors.affiliation: ICAR-Directorate of Medicinal and Aromatic Plant Research, Boriavi, Anand, Gujarat, India.
authors.forename: Anjali
authors.initials: A
authors.lastname: Sharma
authors.affiliation: ICAR-Directorate of Medicinal and Aromatic Plant Research, Boriavi, Anand, Gujarat, India. geethaka99@yahoo.com.
authors.forename: K A
authors.initials: KA
authors.lastname: Geetha
mesh_terms: D001483:Base Sequence; D031743:Cymbopogon / Q000145:classification* / Q000235:genetics; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D018744:DNA, Plant / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000379:methods; D013045:Species Specificity
chemical_list: D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer
keywords: Chloroplast genome; Cymbopogon species; DNA barcode; ITS spacer; MatK