title: Self-targeted salinomycin-loaded DSPE-PEG-methotrexate nanomicelles for targeting both head and neck squamous cell carcinoma cancer cells and cancer stem cells.
abstract: OBJECTIVE
  To target both head and neck squamous cell carcinoma (HNSCC) cells and cancer stem cells (CSCs) by salinomycin-loaded DSPE-PEG-MTX (synthesized using DSPE-PEG2000-NH2 and methotrexate) nanomicelles (M-SAL-MTX).
  
  
  METHODS
  The characterization, antitumor activity and mechanism of M-SAL-MTX were evaluated.
  
  
  CONCLUSIONS
  M-SAL-MTX showed enhanced inhibitory effect toward both HNSCC CSCs and non-CSCs compared with a single treatment of methotrexate and salinomycin. In nude mice-bearing HNSCC xenografts, M-SAL-MTX suppressed tumor growth more effectively than other controls including combination of methotrexate and salinomycin. Therefore, M-SAL-MTX may provide a strategy for treating HNSCC by targeting both HNSCC CSCs and HNSCC cells.
authors.affiliation: Department of Otolaryngology Head & Neck Surgery, Shanghai Changhai Hospital, the Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.
authors.forename: Minhui
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Department of Otolaryngology Head & Neck Surgery, Shanghai Changhai Hospital, the Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.
authors.forename: Shicai
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Otolaryngology Head & Neck Surgery, Shanghai Changhai Hospital, the Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.
authors.forename: Libo
authors.initials: L
authors.lastname: Hua
authors.affiliation: Department of Otolaryngology Head & Neck Surgery, Shanghai Changhai Hospital, the Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.
authors.forename: Caiyun
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology Head & Neck Surgery, Shanghai Changhai Hospital, the Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.
authors.forename: Mengjie
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Otolaryngology Head & Neck Surgery, Shanghai Changhai Hospital, the Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.
authors.forename: Donghui
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Otolaryngology Head & Neck Surgery, Shanghai Changhai Hospital, the Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.
authors.forename: Yinmei
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Department of Otolaryngology Head & Neck Surgery, Shanghai Changhai Hospital, the Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology Head & Neck Surgery, Shanghai Changhai Hospital, the Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Otolaryngology Head & Neck Surgery, Shanghai Changhai Hospital, the Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.
authors.forename: Xianmin
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of Otolaryngology Head & Neck Surgery, Shanghai Changhai Hospital, the Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.|Sunlipo Biotech Research Center for Nanomedicine, 3688 Tingwei Road, Shanghai 201507, China.
authors.forename: Huaiwen
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Otolaryngology Head & Neck Surgery, Shanghai Changhai Hospital, the Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Zheng
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D006258:Head and Neck Neoplasms / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D008297:Male; D008727:Methotrexate / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008823:Micelles; D014411:Neoplastic Stem Cells / Q000187:drug effects* / Q000473:pathology; D010714:Phosphatidylethanolamines / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011714:Pyrans / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D000077195:Squamous Cell Carcinoma of Head and Neck; D000095662:Polyether Polyketides
chemical_list: D000900:Anti-Bacterial Agents; D000964:Antimetabolites, Antineoplastic; D004337:Drug Carriers; D008727:Methotrexate; D008823:Micelles; D010714:Phosphatidylethanolamines; D011092:Polyethylene Glycols; D011714:Pyrans; C038089:1,2-distearoylphosphatidylethanolamine; C010327:salinomycin; D000095662:Polyether Polyketides
keywords: cancer stem cell; head and neck squamous cell carcinoma; methotrexate; nanomicelles; salinomycin