title: Hydrogen sulfide attenuates gastric mucosal injury induced by restraint water-immersion stressviaactivation of KATPchannel and NF-κB dependent pathway.
abstract: OBJECTIVE
  To explore the effect of hydrogen sulfide (H2S) on restraint water-immersion stress (RWIS)-induced gastric lesions in rats and the influence of adenosine triphosphate (ATP)-sensitive potassium (KATP) channels and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway on such an effect.
  
  
  METHODS
  Male Wistar rats were randomly divided into a control group, a physiological saline (PS) group, a sodium hydrosulfide (NaHS) group, a glibenclamide (Gl) group, Gl plus NaHS group, a pyrrolidine dithiocarbamate (PDTC) group, and a PDTC plus NaHS group. Gastric mucosal injury was induced by RWIS for 3 h in rats, and gastric mucosal damage was analyzed after that. The PS, NaHS (100 μmol/kg body weight), Gl (100 μmol/kg body weight), Gl (100 μmol/kg or 150 μmol/kg body weight) plus NaHS (100 μmol/kg body weight), PDTC (100 μmol/kg body weight), and PDTC (100 μmol/kg body weight) plus NaHS (100 μmol/kg body weight) were respectively injected intravenously before RWIS.
  
  
  RESULTS
  RWIS induced serious gastric lesions in the rats in the PS pretreatment group. The pretreatment of NaHS (a H2S donor) significantly reduced the damage induced by RWIS. The gastric protective effect of the NaHS during RWIS was attenuated by PDTC, an NF-κB inhibitor, and also by glibenclamide, an ATP-sensitive potassium channel blocker, in a dose-dependent manner.
  
  
  CONCLUSIONS
  These results suggest that exogenous H2S plays a protective role against RWIS injury in rats, possibly through modulation of KATPchannel opening and the NF-κB dependent pathway.
authors.affiliation: Hong-Zhao Sun, Shan Zheng, Kai Lu, Feng-Tian Hou, Jie-Xue Bi, Xue-Lian Liu, Shan-Shan Wang, College of Life Science, Qi Lu Normal University, Zhangqiu 250200, Jinan, Shandong Province, China.
authors.forename: Hong-Zhao
authors.initials: HZ
authors.lastname: Sun
authors.affiliation: Hong-Zhao Sun, Shan Zheng, Kai Lu, Feng-Tian Hou, Jie-Xue Bi, Xue-Lian Liu, Shan-Shan Wang, College of Life Science, Qi Lu Normal University, Zhangqiu 250200, Jinan, Shandong Province, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Hong-Zhao Sun, Shan Zheng, Kai Lu, Feng-Tian Hou, Jie-Xue Bi, Xue-Lian Liu, Shan-Shan Wang, College of Life Science, Qi Lu Normal University, Zhangqiu 250200, Jinan, Shandong Province, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Lu
authors.affiliation: Hong-Zhao Sun, Shan Zheng, Kai Lu, Feng-Tian Hou, Jie-Xue Bi, Xue-Lian Liu, Shan-Shan Wang, College of Life Science, Qi Lu Normal University, Zhangqiu 250200, Jinan, Shandong Province, China.
authors.forename: Feng-Tian
authors.initials: FT
authors.lastname: Hou
authors.affiliation: Hong-Zhao Sun, Shan Zheng, Kai Lu, Feng-Tian Hou, Jie-Xue Bi, Xue-Lian Liu, Shan-Shan Wang, College of Life Science, Qi Lu Normal University, Zhangqiu 250200, Jinan, Shandong Province, China.
authors.forename: Jie-Xue
authors.initials: JX
authors.lastname: Bi
authors.affiliation: Hong-Zhao Sun, Shan Zheng, Kai Lu, Feng-Tian Hou, Jie-Xue Bi, Xue-Lian Liu, Shan-Shan Wang, College of Life Science, Qi Lu Normal University, Zhangqiu 250200, Jinan, Shandong Province, China.
authors.forename: Xue-Lian
authors.initials: XL
authors.lastname: Liu
authors.affiliation: Hong-Zhao Sun, Shan Zheng, Kai Lu, Feng-Tian Hou, Jie-Xue Bi, Xue-Lian Liu, Shan-Shan Wang, College of Life Science, Qi Lu Normal University, Zhangqiu 250200, Jinan, Shandong Province, China.
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Wang
mesh_terms: D000818:Animals; D005753:Gastric Mucosa / Q000187:drug effects* / Q000293:injuries; D005905:Glyburide / Q000008:administration & dosage / Q000494:pharmacology; D006862:Hydrogen Sulfide / Q000008:administration & dosage / Q000494:pharmacology*; D007275:Injections, Intravenous; D054086:KATP Channels / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008297:Male; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011759:Pyrrolidines / Q000008:administration & dosage / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D013315:Stress, Psychological / Q000150:complications; D013859:Thiocarbamates / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D054086:KATP Channels; D016328:NF-kappa B; D011759:Pyrrolidines; D013859:Thiocarbamates; C020972:pyrrolidine dithiocarbamic acid; D005905:Glyburide; D006862:Hydrogen Sulfide
keywords: Adenosine triphosphate-sensitive potassium; Gastric mucosal injury; Hydrogen sulfide; Nuclear factor kappa B; Restraint water-immersion stress