title: Development of poly(lactide-co-glycolide) nanoparticles functionalized with a mitochondria penetrating peptide.
abstract: The development of mitochondria-targeting cell permeable vectors represents a promising therapeutic approach for several diseases, such as cancer and oxidative pathologies. Nevertheless, access to mitochondria can be difficult. A new hybrid material composed by poly(lactide-co-glycolide) (PLGA) functionalized with a 6-mer mitochondria penetrating peptide (MPP), consisting in alternating arginine and unnatural cyclohexylalanine, was developed. Circular dichroism, FT-IR and DSC studies indicated that the conjugation of the peptide with the polymer led to the obtainment of a more rigid material with respect to both PLGA and MPP as such. In particular, a conformational rearrangement to a helical structure was observed for MPP. MPP-PLGA conjugates were used for the preparation of nanoparticles that showed no cytotoxicity in MTT assay, suggesting their putative use for future studies on mitochondria targeting. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd.
authors.affiliation: Department of Pharmaceutical Sciences, Università degli Studi di Milano, via G. Colombo, 71, 20133, Milano, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Selmin
authors.affiliation: Department of Pharmaceutical Sciences, Università degli Studi di Milano, via G. Colombo, 71, 20133, Milano, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Magri
authors.affiliation: Department of Pharmaceutical Sciences, Università degli Studi di Milano, via G. Colombo, 71, 20133, Milano, Italy.
authors.forename: Chiara G M
authors.initials: CG
authors.lastname: Gennari
authors.affiliation: Department of Pharmacological and Biomolecular Sciences, via Balzaretti, 9, 20133, Milano, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Marchianò
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Padua, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Ferri
authors.affiliation: Department of Pharmaceutical Sciences, Università degli Studi di Milano, via Venezian, 21, 20133, Milano, Italy.
authors.forename: Sara
authors.identifier: http://orcid.org/0000-0002-2325-3583
authors.initials: S
authors.lastname: Pellegrino
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000494:pharmacology; D005449:Fluorenes / Q000737:chemistry; D045325:HCT116 Cells; D006801:Humans; D008928:Mitochondria / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis* / Q000494:pharmacology; D010539:Permeability; D011098:Polyglactin 910 / Q000138:chemical synthesis* / Q000494:pharmacology; D060327:Solid-Phase Synthesis Techniques / Q000379:methods
chemical_list: D004337:Drug Carriers; D005449:Fluorenes; D010455:Peptides; D011098:Polyglactin 910
keywords: conformational study; hybrid polymers; mitochondria penetrating peptide; nanoparticles; poly(lactide-co-glycolide); polymer-peptide