title: Acute toxicity of chlorpyrifos to embryo and larvae of banded gourami Trichogaster fasciata.
abstract: This study elucidated the acute toxicity of chlorpyrifos on the early life stages of banded gourami (Trichogaster fasciata). To determine the acute effects of chlorpyrifos on their survival and development, we exposedthe embryos and two-day-old larvae to six concentrations (0, 0.01, 0.10, 1.0, 10 and 100 µg L-1) of chlorpyrifos in plastic bowls. Log-logistic regression was used to calculate LC10 and LC50 values. Results showed that embryo mortality significantly increased with increasing chlorpyrifos concentrations. The 24-h LC10 and LC50 values (with 95% confidence limits) of chlorpyrifos for embryos were 0.89 (0.50-1.58) and 11.8 (9.12-15.4) µg L-1, respectively. Hatching success decreased and mortality of larvae significantly increased with increasing concentrations of chlorpyrifos. The 24-h LC10 and LC50 values (with 95% confidence limits) of chlorpyrifos for larvae were 0.53 (0.27-1.06) and 21.7 (15.9-29.4) µg L-1, respectively; the 48-h LC10 and LC50 for larvae were 0.04 (0.02-0.09) and 5.47 (3.77-7.94) µg L-1, respectively. The results of this study suggest that 1 µg L-1of chlorpyrifos in the aquatic environment may adversely affect the development and the reproduction of banded gourami. Our study also suggests that banded gourami fish can serve as an ideal model species for evaluating developmental toxicity of environmental contaminants.
authors.affiliation: a Department of Aquatic Ecology and Water Quality Management , Wageningen University, Wageningen University and Research Centre , Wageningen , The Netherlands.|b Department of Fisheries Management , Bangladesh Agricultural University , Mymensingh , Bangladesh.
authors.forename: Kizar Ahmed
authors.initials: KA
authors.lastname: Sumon
authors.affiliation: b Department of Fisheries Management , Bangladesh Agricultural University , Mymensingh , Bangladesh.
authors.forename: Sampa
authors.initials: S
authors.lastname: Saha
authors.affiliation: a Department of Aquatic Ecology and Water Quality Management , Wageningen University, Wageningen University and Research Centre , Wageningen , The Netherlands.|c Alterra, Wageningen University and Research Centre , Wageningen , The Netherlands.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: van den Brink
authors.affiliation: a Department of Aquatic Ecology and Water Quality Management , Wageningen University, Wageningen University and Research Centre , Wageningen , The Netherlands.
authors.forename: Edwin T H M
authors.initials: ET
authors.lastname: Peeters
authors.affiliation: d Aquaculture and Fisheries, Wageningen University, Wageningen University and Research Centre , Wageningen , The Netherlands.
authors.forename: Roel H
authors.initials: RH
authors.lastname: Bosma
authors.affiliation: b Department of Fisheries Management , Bangladesh Agricultural University , Mymensingh , Bangladesh.|e Science and Math Program, Asian University for Women , Chittagong , Bangladesh.
authors.forename: Harunur
authors.initials: H
authors.lastname: Rashid
mesh_terms: D000818:Animals; D001459:Bangladesh; D004390:Chlorpyrifos / Q000008:administration & dosage / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian / Q000187:drug effects; D005260:Female; D007814:Larva / Q000187:drug effects; D008297:Male; D010473:Perciformes / Q000196:embryology*; D010575:Pesticides / Q000633:toxicity; D023382:Toxicity Tests, Acute / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D010575:Pesticides; D014874:Water Pollutants, Chemical; D004390:Chlorpyrifos
keywords: Organophosphorous pesticide; aquatic environment; banded gourami; developmental toxicity; malformation