title: Canna edulisLeaf Extract-Mediated Preparation of Stabilized Silver Nanoparticles: Characterization, Antimicrobial Activity, and Toxicity Studies.
abstract: A novel approach to synthesize silver nanoparticles (AgNPs) using leaf extract ofCanna edulisKer-Gawl. (CELE) under ambient conditions is reported here. The as-prepared AgNPs were analyzed by UV-visible spectroscopy, transmission emission microscopy, X-ray diffraction, Fourier transform-infrared spectroscopy, energy-dispersive analysis of X-ray spectroscopy, zeta potential, and dynamic light scattering. The AgNPs showed excellent antimicrobial activity against various pathogens, including bacteria and various fungi. The biocompatibility of the AgNPs was analyzed in the L929 cell line using NRU and MTT assays. Acridine orange/ethidium bromide staining was used to determine whether the AgNPs had necrotic or apoptotic effects on L929 cells. The concentration of AgNPs required for 50% inhibition of growth of mammalian cells is far more than that required for inhibition of pathogenic microorganisms. Thus, CELE is a candidate for the eco-friendly, clean, cost-effective, and nontoxic synthesis of AgNPs.
authors.affiliation: Department of Chemical Engineering, Konkuk University, Seoul 05029, Republic of Korea.|Center for Interdisciplinary Research, D. Y. Patil University, Kolhapur-416 006, Maharashtra State, India.
authors.forename: S V
authors.initials: SV
authors.lastname: Otari
authors.affiliation: Center for Interdisciplinary Research, D. Y. Patil University, Kolhapur-416 006, Maharashtra State, India.
authors.forename: S H
authors.initials: SH
authors.lastname: Pawar
authors.affiliation: Department of Chemical Engineering, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Sanjay K S
authors.initials: SKS
authors.lastname: Patel
authors.affiliation: Department of Chemical Engineering, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Raushan K
authors.initials: RK
authors.lastname: Singh
authors.affiliation: Department of Food Science and Biotechnology, Shin-Ansan University, Ansan 15435, Republic of Korea.
authors.forename: Sang-Yong
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Department of Mechanical Engineering, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Jai Hyo
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Sciences, Gutian Edible Fungi Research Institute, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province 350002, P.R. China.
authors.forename: Liaoyuan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Chemical Engineering, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Jung-Kul
authors.initials: JK
authors.lastname: Lee
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology* / Q000633:toxicity; D001419:Bacteria / Q000187:drug effects; D002460:Cell Line / Q000187:drug effects; D000067493:Dynamic Light Scattering / Q000379:methods; D005658:Fungi / Q000187:drug effects; D055772:Green Chemistry Technology / Q000379:methods*; D008422:Materials Testing / Q000379:methods; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission / Q000379:methods; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D056928:X-Ray Absorption Spectroscopy / Q000379:methods; D014961:X-Ray Diffraction / Q000379:methods; D019862:Zingiberales / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D010936:Plant Extracts; D012834:Silver
keywords: Canna edulis Ker-Gawl.; Green chemistry; antimicrobial; biocompatible; nanobiotechnology