title: A novel mitochondria-targeting fluorescent probe for hydrogen sulfide in living cells.
abstract: A novel mitochondria-targeting fluorescent probe compound S-N3for hydrogen sulfide (H2S) in living cells has been designed and synthesized in this study. This article contained the chemosynthesis and some studies on bioactivity of the target compound in living cells. Compound S-N3is easy to synthesize and can remain stable under the effect of pH, system and photostability. In addition, it shows low cytotoxicity in cell imaging. And it can react with H2S highly selective in PBS or FBS solution, which would cause the obvious increase in fluorescence intensity. Therefore, the low-cost detection method for H2S is allowed for monitoring the quantity of H2S existed in the mitochondria.
authors.affiliation: Center of Drug Discovery, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.affiliation: Center of Drug Discovery, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China.
authors.forename: Miaobo
authors.initials: M
authors.lastname: Pan
authors.affiliation: Center of Drug Discovery, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Qiang
authors.affiliation: Center of Drug Discovery, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Qiu
authors.affiliation: Center of Drug Discovery, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China.|Jiangsu Key Laboratory of Drug Discovery for Metabolic Disease, China Pharmaceutical University, Nanjing, China.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Huang
authors.affiliation: Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Lin
authors.affiliation: Center of Drug Discovery, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China.|Jiangsu Key Laboratory of Drug Discovery for Metabolic Disease, China Pharmaceutical University, Nanjing, China.
authors.forename: Hai
authors.identifier: 0000-0002-3827-0992
authors.initials: H
authors.lastname: Qian
authors.affiliation: Center of Drug Discovery, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Ge
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D061986:MCF-7 Cells; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000737:chemistry* / Q000648:ultrastructure; D061848:Optical Imaging; D010782:Photolysis; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D006862:Hydrogen Sulfide
keywords: fluorescent probe; hydrogen sulfide; mitochondria targeting; selectively