title: Early Changes of Peripheral Blood Lymphocyte Subpopulations in Patients with Occupational 2,4-dinitrophenol Poisoning.
abstract: 2,4-dinitrophenol (DNP), an organic compound which frequently used in industry, is considered to have high toxicity. This study aimed to investigate the early changes of lymphocyte subpopulations in patients with occupational 2,4-DNP poisoning. Totally 9 patients with acute occupational 2,4-DNP poisoning and 30 healthy volunteers as control were enrolled. The patients received immediately comprehensive supportive treatments, including large-dose glucocorticoid and repeated hemoperfusion (HP). The ratio of CD4+/CD8+ T cells were significantly higher in patients upon admission compared to healthy controls (P < 0.01); however, counts of total lymphocytes, CD3+, CD3+CD4+, CD3+CD8+, B (CD19+), and natural killer (NK) cells (CD16+CD56+) were significantly reduced (all P < 0.001). The NK cell count was negatively correlated with initial plasma 2,4-DNP concentration (r = -0.750, P = 0.026). Thus, acute occupational 2,4-DNP poisoning was accompanied by immediate complex immune cell reactions, especially NK cells might play important role in severe 2,4-DNP poisoning.
authors.affiliation: Department of Emergency Medicine, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang, China.
authors.forename: Jiu Kun
authors.initials: JK
authors.lastname: Jiang
authors.affiliation: Department of Emergency Medicine, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Fang
authors.affiliation: Cancer Institute, Zhejiang Tumor Hospital, Hangzhou 310022, Zhejiang, China.
authors.forename: Lin Hui
authors.initials: LH
authors.lastname: Gu
authors.affiliation: Department of Emergency Medicine, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang, China.
authors.forename: Yuan Qiang
authors.initials: YQ
authors.lastname: Lu
mesh_terms: D019297:2,4-Dinitrophenol / Q000506:poisoning* / Q000633:toxicity; D000328:Adult; D002681:China; D004396:Coloring Agents / Q000506:poisoning* / Q000633:toxicity; D005260:Female; D006801:Humans; D007694:Killer Cells, Natural / Q000187:drug effects; D016131:Lymphocyte Subsets / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D009784:Occupational Diseases / Q000139:chemically induced*; D013601:T-Lymphocytes / Q000187:drug effects
chemical_list: D004396:Coloring Agents; D019297:2,4-Dinitrophenol