title: The RFamide receptor DMSR-1 regulates stress-induced sleep inC. elegans.
abstract: In response to environments that cause cellular stress, animals engage in sleep behavior that facilitates recovery from the stress. InCaenorhabditis elegans, stress-induced sleep(SIS) is regulated by cytokine activation of the ALA neuron, which releases FLP-13 neuropeptides characterized by an amidated arginine-phenylalanine (RFamide) C-terminus motif. By performing an unbiased genetic screen for mutants that impair the somnogenic effects of FLP-13 neuropeptides, we identified the genedmsr-1, which encodes a G-protein coupled receptor similar to an insect RFamide receptor. DMSR-1 is activated by FLP-13 peptides in cell culture, is required for SISin vivo, is expressed non-synaptically in several wake-promoting neurons, and likely couples to a Gi/o heterotrimeric G-protein. Our data expand our understanding of how a single neuroendocrine cell coordinates an organism-wide behavioral response, and suggest that similar signaling principles may function in other organisms to regulate sleep during sickness.
authors.affiliation: Department of Neurology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States.|Center for Sleep and Circadian Neurobiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Iannacone
authors.affiliation: Department of Biology, Katholieke Universiteit Leuven, Leuven, Belgium.
authors.forename: Isabel
authors.initials: I
authors.lastname: Beets
authors.affiliation: Department of Neurology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States.|Center for Sleep and Circadian Neurobiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States.
authors.forename: Lindsey E
authors.initials: LE
authors.lastname: Lopes
authors.affiliation: Department of Bioengineering, School of Engineering and Applied Sciences, University of Pennsylvania, Philadelphia, United States.
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Churgin
authors.affiliation: Department of Bioengineering, School of Engineering and Applied Sciences, University of Pennsylvania, Philadelphia, United States.
authors.forename: Christopher
authors.initials: C
authors.lastname: Fang-Yen
authors.affiliation: Department of Biology, Saint Joseph's University, Philadelphia, United States.
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Nelson
authors.affiliation: Department of Biology, Katholieke Universiteit Leuven, Leuven, Belgium.
authors.forename: Liliane
authors.initials: L
authors.lastname: Schoofs
authors.affiliation: Department of Neurology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States.|Center for Sleep and Circadian Neurobiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States.
authors.forename: David M
authors.identifier: 0000-0001-5935-0476
authors.initials: DM
authors.lastname: Raizen
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism*; D005786:Gene Expression Regulation; D009479:Neuropeptides / Q000378:metabolism*; D018000:Receptors, Peptide / Q000378:metabolism*; D012890:Sleep; D013312:Stress, Physiological
chemical_list: D029742:Caenorhabditis elegans Proteins; D009479:Neuropeptides; D018000:Receptors, Peptide; C058047:arginylphenylalaninamide
keywords: C. elegans; GPCR; RFamide; neuropeptides; neuroscience; sickness; sleep