title: Simultaneous detection of three sex steroid hormone classes using a novel yeast-based biosensor.
abstract: A biosensor detecting estrogens, progestogens, and androgens in complex samples and in a single step is described. Three Arxula adeninivorans yeast strains were created, each strain producing a different recombinant human hormone receptor and a different fluorescent reporter protein. These strains were then mixed to create G1212/YRC102-hHR-fluo, the biological component of the biosensor. During incubation with G1212/YRC102-hHR-fluo, hormones present in a sample bind to their target receptor, which leads to the production of a specific fluorescent protein. Three fluorescence scans of the yeast suspension determine which fluorescence protein has been produced, thus revealing which hormone receptor (estrogen, progesterone, and androgen) has been activated by the hormones or hormone mimics present in the sample. The biosensor has similar sensitivities to the existing A. adeninivorans cell-based assays. The detection of the three hormone classes in one single experiment reduces the labor and time required to assay for the three hormone classes. The biosensor was also trialed with animal serum samples for the detection of progestogens, androgens, and estrogens and gave results that correlated well with ELISA analysis in case of progestogens. These results highlight the potential usefulness of the biosensor for comprehensive determination of hormone status in samples from veterinary origin. Biotechnol. Bioeng. 2017;114: 1539-1549. © 2017 Wiley Periodicals, Inc.
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, Gatersleben D-06466, Germany.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Chamas
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, Gatersleben D-06466, Germany.|Institute of Biotechnology, Vietnam Academy of Science and Technology, Cau Giay, Ha Noi, Vietnam.
authors.forename: Ha Thi Minh
authors.initials: HTM
authors.lastname: Pham
authors.affiliation: QuoData GmbH, Dresden, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Jähne
authors.affiliation: QuoData GmbH, Dresden, Germany.
authors.forename: Karina
authors.initials: K
authors.lastname: Hettwer
authors.affiliation: QuoData GmbH, Dresden, Germany.
authors.forename: Steffen
authors.initials: S
authors.lastname: Uhlig
authors.affiliation: New diagnostics GmbH, Freising, Germany.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Simon
authors.affiliation: Faculty of Veterinary Medicine, Institute of Physiological Chemistry, Leipzig, Germany.
authors.forename: Almuth
authors.initials: A
authors.lastname: Einspanier
authors.affiliation: School of Biological Sciences, University of Canterbury, Christchurch, New Zealand.
authors.forename: Kim
authors.initials: K
authors.lastname: Baronian
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, Gatersleben D-06466, Germany.
authors.forename: Gotthard
authors.initials: G
authors.lastname: Kunze
mesh_terms: D000818:Animals; D001203:Ascomycota / Q000145:classification* / Q000187:drug effects*; D001681:Biological Assay / Q000295:instrumentation*; D015374:Biosensing Techniques / Q000295:instrumentation*; D002144:Callithrix; D004867:Equipment Design; D019544:Equipment Failure Analysis; D012739:Gonadal Steroid Hormones / Q000032:analysis / Q000097:blood* / Q000494:pharmacology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation
chemical_list: D012739:Gonadal Steroid Hormones
keywords: Arxula adeninivorans; fluorescence; hormone assay; marmoset serum