title: Proteomic changes in the ileum of sheep infected with Mycobacterium avium subspecies paratuberculosis.
abstract: Johne's disease (JD) is a chronic enteritis of ruminants caused by Mycobacterium avium subspecies paratuberculosis (MAP). To identify the processes activated in the sheep intestine during natural MAP infection, and to provide a panel of differential host and pathogen proteins with diagnostic and prognostic potential, a differential shotgun proteomics workflow, including mass spectrometry, label-free quantisation and pathway analysis, was applied to ileal tissues of ewes with and without JD. Out of 2889 total proteins identified, 384 were differentially expressed and 341 were expressed at a higher level in JD. On the basis of Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) analysis, these proteins were involved in numerous relevant biological networks and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including inhibition of phagosome acidification (such as V-ATPase), bacterial invasion, leucocyte recruitment and activation, and antimicrobial activity (such as haptoglobin, lactoferrin, cathelicidins, calgranulins and interleukins). A total of 28 MAP proteins were identified, including bacterioferritin, β-lactamase and heparin-binding haemagglutinin (HBHA), a mycobacterial adhesin crucial for dissemination of infection.
authors.affiliation: Porto Conte Ricerche, S.P. 55 Porto Conte/Capo Caccia Km 8.400, Loc. Tramariglio, 07041 Alghero, SS, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Pisanu
authors.affiliation: Dipartimento di Medicina Veterinaria, Università di Sassari, Via Vienna 2, 07100 Sassari, Italy.
authors.forename: T
authors.initials: T
authors.lastname: Cubeddu
authors.affiliation: Porto Conte Ricerche, S.P. 55 Porto Conte/Capo Caccia Km 8.400, Loc. Tramariglio, 07041 Alghero, SS, Italy; Dipartimento di Scienze Biomediche, Università di Sassari, Viale S. Pietro 43/B, 07100 Sassari, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Uzzau
authors.affiliation: Dipartimento di Medicina Veterinaria, Università di Sassari, Via Vienna 2, 07100 Sassari, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Rocca
authors.affiliation: Porto Conte Ricerche, S.P. 55 Porto Conte/Capo Caccia Km 8.400, Loc. Tramariglio, 07041 Alghero, SS, Italy. Electronic address: addis@portocontericerche.it.
authors.forename: M F
authors.initials: MF
authors.lastname: Addis
mesh_terms: D000818:Animals; D005260:Female; D015870:Gene Expression; D007082:Ileum / Q000378:metabolism* / Q000382:microbiology; D016927:Mycobacterium avium subsp. paratuberculosis / Q000502:physiology*; D010283:Paratuberculosis / Q000235:genetics* / Q000382:microbiology; D020543:Proteome; D040901:Proteomics; D012756:Sheep; D012757:Sheep Diseases / Q000235:genetics* / Q000382:microbiology
chemical_list: D020543:Proteome
keywords: Johne's disease; Mass spectrometry; Mycobacterium avium subspecies paratuberculosis; Ovine; Proteomics