title: Metformin and low dose radiation modulates cisplatin-induced oxidative injury in rat via PPAR-γ and MAPK pathways.
abstract: Cisplatin (CIS) is a chemotherapeutic agent used for therapy of many tumors and has been limited by its toxicity. The aim of this study was to investigate the role of Peroxisome proliferator-activated receptor-gamma (PPAR-γ), mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B(NFkB) in the pathogenesis of hepatic damage induced by CIS, and investigated the modulatory effect of metformin (MET) and/or low dose gamma radiation (LDR) on CIS-induced hepatotoxicity in rats. CIS(7.5 mg/kg, i.p.) hepatotoxicity was evidenced by alteration of serum hepatic indices (ALT and AST) accompanied with decreased hepatic PPAR-γ, superoxide dismutase (SOD) activities and reduced glutathione (GSH) content, whereas the levels of malondialdehyde (MDA), total nitrate/nitrite (NOx) and NFkB significantly increased as well as MAPK activity compared with the control, MET and LDR groups. Furthermore, CIS induces apoptosis as indicated by an elevation of hepatic caspase-3. Treatment with MET (150 mg/kg, orally for 14 days) and/or LDR (0.5 Gy), prior to CIS alleviates CIS-induced hepatic damage by mitigating oxidative/ nitrosative stress and PPAR-γ activity reduction, hepatic caspase-3 elevation, and inhibition of NFκB, and MAPK activity levels.
  
  
  
  Modulation of PPAR-γ, MAPK and NFkB might contribute to amelioration of CIS-induced hepatic toxicity.
authors.affiliation: Health Radiation Research Department, National Center for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt. Electronic address: hebahosny@hotmail.com.
authors.forename: Heba H
authors.initials: HH
authors.lastname: Mansour
authors.affiliation: Health Radiation Research Department, National Center for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt. Electronic address: shereenelkiki@yahoo.com.
authors.forename: Shereen M
authors.initials: SM
authors.lastname: El Kiki
authors.affiliation: Health Radiation Research Department, National Center for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt. Electronic address: shereen.galal@yahoo.com.
authors.forename: Shereen M
authors.initials: SM
authors.lastname: Galal
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000009:adverse effects; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D002945:Cisplatin / Q000009:adverse effects*; D005786:Gene Expression Regulation; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D020935:MAP Kinase Signaling System; D008297:Male; D008687:Metformin / Q000737:chemistry*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress; D047495:PPAR gamma / Q000378:metabolism*; D011829:Radiation Dosage; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D016328:NF-kappa B; D047495:PPAR gamma; D008687:Metformin; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D020928:Mitogen-Activated Protein Kinases; D053148:Caspase 3; D005978:Glutathione; D002945:Cisplatin
keywords: Cisplatin; Low dose radiation; MAPK; Metformin; NF(k)B; PPAR-γ