title: Dysregulated circulating miRNAs in preeclampsia.
abstract: Preeclampsia (PE) is a pregnancy-related disease with potentially severe consequences with respect to foeto-maternal morbidity and mortality. However, the molecular pathogenesis of PE remains largely unknown. Recent reports have shown that microRNAs (miRNAs or miRs) may play important roles in the development of PE. Analysing the miRNAs in sera from preeclamptic women may improve our understanding of the pathophysiological mechanisms of the disease. The aim of this retrospective study was to identify whether circulating miRNAs were differentially expressed in PE patients compared with controls. Serum samples from 23 women who developed PE were compared with samples from 44 pregnant controls. Seventeen circulating miRNAs previously described in PE were chosen for evaluation of their expression by reverse transcription quantitative polymerase chain reaction (RT-qPCR). In the maternal serum, the miR-210-3p, miR-210-5p, miR-1233-3p, and miR-574-5p levels were found to be significantly higher in the PE patients than in the controls (P<0.05). Using a logistic regression model, we evaluated the discriminant power of those differentially expressed miRNAs, and the combination of miR-210-5p and miR-574-5p yielded an area under the curve of 0.7223 for discriminating PE patients from the controls. In conclusion, the fact that four circulating miRNAs (miR-210-3p, miR-210-5p, miR-1233-3p, and miR-574-5p) were differentially expressed in the sera of women who developed PE compared with controls confirms the possible pathophysiological role of miRNAs in PE.
authors.affiliation: Laboratory of Tumor and Development Biology, GIGA-R, University of Liège, B-4000 Liège, Belgium.
authors.forename: Carine
authors.initials: C
authors.lastname: Munaut
authors.affiliation: Department of Obstetrics and Gynecology, University of Liège, Hôpital de la Citadelle, B-4000 Liège, Belgium.
authors.forename: Linda
authors.initials: L
authors.lastname: Tebache
authors.affiliation: Laboratory of Tumor and Development Biology, GIGA-R, University of Liège, B-4000 Liège, Belgium.
authors.forename: Silvia
authors.initials: S
authors.lastname: Blacher
authors.affiliation: Laboratory of Tumor and Development Biology, GIGA-R, University of Liège, B-4000 Liège, Belgium.
authors.forename: Agnès
authors.initials: A
authors.lastname: Noël
authors.affiliation: Department of Obstetrics and Gynecology, University of Liège, Hôpital de la Citadelle, B-4000 Liège, Belgium.
authors.forename: Michelle
authors.initials: M
authors.lastname: Nisolle
authors.affiliation: Department of Obstetrics and Gynecology, University of Liège, Hôpital de la Citadelle, B-4000 Liège, Belgium.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Chantraine
keywords: RT-qPCR; logistic regression model; miRNAs; preeclampsia