title: Glucose-6-phosphate dehydrogenase deficiency does not increase the susceptibility of sperm to oxidative stress induced by H2O2.
abstract: OBJECTIVE
  Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common human enzyme defect. G6PD plays a key role in the pentose phosphate pathway, which is a major source of nicotinamide adenine dinucleotide phosphate (NADPH). NADPH provides the reducing equivalents for oxidation-reduction reductions involved in protecting against the toxicity of reactive oxygen species such as H2O2. We hypothesized that G6PD deficiency may reduce the amount of NADPH in sperms, thereby inhibiting the detoxification of H2O2, which could potentially affect their motility and viability, resulting in an increased susceptibility to infertility.
  
  
  METHODS
  Semen samples were obtained from four males with G6PD deficiency and eight healthy males as a control. In both groups, motile sperms were isolated from the seminal fluid and incubated with 0, 10, 20, 40, 60, 80, and 120 µM concentrations of H2O2. After 1 hour incubation at 37℃, sperms were evaluated for motility and viability.
  
  
  RESULTS
  Incubation of sperms with 10 and 20 µM H2O2led to very little decrease in motility and viability, but motility decreased notably in both groups in 40, 60, and 80 µM H2O2, and viability decreased in both groups in 40, 60, 80, and 120 µM H2O2. However, no statistically significant differences were found between the G6PD-deficient group and controls.
  
  
  CONCLUSIONS
  G6PD deficiency does not increase the susceptibility of sperm to oxidative stress induced by H2O2, and the reducing equivalents necessary for protection against H2O2are most likely produced by other pathways. Therefore, G6PD deficiency cannot be considered as major risk factor for male infertility.
authors.affiliation: Department of Anatomical Sciences and Biology, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Shiva
authors.initials: S
authors.lastname: Roshankhah
authors.affiliation: Molecular Pathology Research Center, Imam Reza Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Rostami-Far
authors.affiliation: Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Farhad
authors.initials: F
authors.lastname: Shaveisi-Zadeh
authors.affiliation: Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Movafagh
authors.affiliation: Department of Anatomical Sciences and Biology, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Mitra
authors.initials: M
authors.lastname: Bakhtiari
authors.affiliation: Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Jila
authors.initials: J
authors.lastname: Shaveisi-Zadeh
keywords: Glucose-6-phosphate dehydrogenase deficiency; Infertility; Oxidative stress; Risk factors