title: Genome engineering of stem cell organoids for disease modeling.
abstract: Precision medicine emerges as a new approach that takes into account individual variability. Successful realization of precision medicine requires disease models that are able to incorporate personalized disease information and recapitulate disease development processes at the molecular, cellular and organ levels. With recent development in stem cell field, a variety of tissue organoids can be derived from patient specific pluripotent stem cells and adult stem cells. In combination with the state-of-the-art genome editing tools, organoids can be further engineered to mimic disease-relevant genetic and epigenetic status of a patient. This has therefore enabled a rapid expansion of sophisticated in vitro disease models, offering a unique system for fundamental and biomedical research as well as the development of personalized medicine. Here we summarize some of the latest advances and future perspectives in engineering stem cell organoids for human disease modeling.
authors.affiliation: CAS Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China.|University of Chinese Academy of Sciences, Shanghai, 200031, China.
authors.forename: Yingmin
authors.initials: Y
authors.lastname: Sun
authors.affiliation: CAS Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China. qrding@sibs.ac.cn.|University of Chinese Academy of Sciences, Shanghai, 200031, China. qrding@sibs.ac.cn.
authors.forename: Qiurong
authors.initials: Q
authors.lastname: Ding
mesh_terms: D000818:Animals; D035843:Biomedical Research; D000072669:Gene Editing / Q000379:methods*; D006801:Humans; D008954:Models, Biological; D009940:Organoids / Q000378:metabolism*; D039904:Pluripotent Stem Cells / Q000378:metabolism*; D057285:Precision Medicine / Q000379:methods*
keywords: genome editing; pluripotent/adult stem cell; precision medicine; tissue organoid