title: Integrated Physiological, Proteomic, and Metabolomic Analysis of Ultra Violet (UV) Stress Responses and Adaptation Mechanisms inPinus radiata.
abstract: Globally expected changes in environmental conditions, especially the increase of UV irradiation, necessitate extending our knowledge of the mechanisms mediating tree species adaptation to this stress. This is crucial for designing new strategies to maintain future forest productivity. Studies focused on environmentally realistic dosages of UV irradiation in forest species are scarce.Pinus spp.are commercially relevant trees and not much is known about their adaptation to UV. In this work, UV treatment and recovery ofPinus radiataplants with dosages mimicking future scenarios, based on current models of UV radiation, were performed in a time-dependent manner. The combined metabolome and proteome analysis were complemented with measurements of + physiological parameters and gene expression. Sparse PLS analysis revealed complex molecular interaction networks of molecular and physiological data. Early responses prevented phototoxicity by reducing photosystem activity and the electron transfer chain together with the accumulation of photoprotectors and photorespiration. Apart from the reduction in photosynthesis as consequence of the direct UV damage on the photosystems, the primary metabolism was rearranged to deal with the oxidative stress while minimizing ROS production. New protein kinases and proteases related to signaling, coordination, and regulation of UV stress responses were revealed. All these processes demonstrate a complex molecular interaction network extending the current knowledge on UV-stress adaptation in pine.
authors.affiliation: From the ‡Plant Physiology Lab, Organisms and Systems Biology, Faculty of Biology, University of Oviedo, Oviedo, Asturias, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Pascual
authors.affiliation: From the ‡Plant Physiology Lab, Organisms and Systems Biology, Faculty of Biology, University of Oviedo, Oviedo, Asturias, Spain.
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Cañal
authors.affiliation: From the ‡Plant Physiology Lab, Organisms and Systems Biology, Faculty of Biology, University of Oviedo, Oviedo, Asturias, Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: Escandón
authors.affiliation: From the ‡Plant Physiology Lab, Organisms and Systems Biology, Faculty of Biology, University of Oviedo, Oviedo, Asturias, Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: Meijón
authors.affiliation: §Department of Ecogenomics and Systems Biology, Faculty of Life Sciences, University of Vienna, Vienna, Austria.|¶Vienna Metabolomics Center (VIME), University of Vienna, Vienna, Austria.
authors.forename: Wolfram
authors.initials: W
authors.lastname: Weckwerth
authors.affiliation: From the ‡Plant Physiology Lab, Organisms and Systems Biology, Faculty of Biology, University of Oviedo, Oviedo, Asturias, Spain; valledor.luis@uniovi.es.
authors.forename: Luis
authors.identifier: 0000-0002-0636-365X
authors.initials: L
authors.lastname: Valledor
mesh_terms: D000222:Adaptation, Physiological / Q000528:radiation effects*; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D055432:Metabolomics / Q000379:methods*; D018384:Oxidative Stress; D010788:Photosynthesis / Q000528:radiation effects; D028223:Pinus / Q000378:metabolism / Q000528:radiation effects*; D018515:Plant Leaves / Q000378:metabolism / Q000528:radiation effects; D010940:Plant Proteins / Q000378:metabolism* / Q000528:radiation effects; D060066:Protein Interaction Maps / Q000528:radiation effects; D040901:Proteomics / Q000379:methods*; D011829:Radiation Dosage; D013997:Time Factors
chemical_list: D010940:Plant Proteins