title: Proteomic Analysis Reveals Proteins Involved in Seed Imbibition under Salt Stress in Rice.
abstract: Enhancement of salinity tolerance during seed germination is very important for direct seeding in rice. In this study, the salt-tolerantjaponicalandrace Jiucaiqing was used to determine the regulators that are involved in seed imbibition under salt stress. Briefly, the comparative proteomic analysis was conducted between dry (0 h) and imbibed (24 h) seeds with 150 mM NaCl. Under salt stress, the uptake of water increased rapidly before 24 h imbibition (Phase I), followed by a plateau of seed imbibition from 24 to 96 h imbibition (Phase II). We identified 14 proteins involved in seed imbibition, in which the majority of these proteins were involved in energy supply and storage protein. The early imbibition process was mediated by protein catabolism; the most of proteins were down-regulated after 24 h imbibition. Eleven genes in salt stress treated seeds were expressed early during the seed imbibition in comparison to control seeds. By comparison, 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (BPM), glutelin (GLU2.2andGLU2.3), glucose-1-phosphate adenylyltransferase large subunit (GAS8), and cupin domain containing protein (CDP3.1andCDP3.2) were near the regions of quantitative trait loci (QTLs) for seed dormancy, seed reserve utilization, and seed germination in Jiucaiqing. In particular,CDP3.1was co-located in the region ofqIR-3for imbibition rate, andqGP-3for germination percentage. The role ofCDP3.1was verified in enhancing seed germination under salt stress using T-DNA mutant. The identified proteins might be applicable for the improvement of seed germination under salt stress in rice.
authors.affiliation: The Laboratory of Seed Science and Technology, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University Nanjing, China.
authors.forename: Enshun
authors.initials: E
authors.lastname: Xu
authors.affiliation: Department of Plant Science, College of Biological Sciences, Henan Agricultural University Zhengzhou, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Chen
authors.affiliation: The Laboratory of Seed Science and Technology, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University Nanjing, China.
authors.forename: Hui
authors.initials: H
authors.lastname: He
authors.affiliation: The Laboratory of Seed Science and Technology, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University Nanjing, China.
authors.forename: Chengfang
authors.initials: C
authors.lastname: Zhan
authors.affiliation: The Laboratory of Seed Science and Technology, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University Nanjing, China.
authors.forename: Yanhao
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: The Laboratory of Seed Science and Technology, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University Nanjing, China.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Zhang
authors.affiliation: The Laboratory of Seed Science and Technology, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University Nanjing, China.
authors.forename: Zhoufei
authors.initials: Z
authors.lastname: Wang
keywords: comparative proteomics; rice; salt stress; seed germination; seed imbibition