title: Lack of Association of CD55 Receptor Genetic Variants and Severe Malaria in Ghanaian Children.
abstract: In a recent report, the cellular receptor CD55 was identified as a molecule essential for the invasion of human erythrocytes byPlasmodium falciparum, the causal agent of the most severe form of malaria. As this invasion process represents a critical step during infection with the parasite, it was hypothesized that genetic variants in the gene could affect severe malaria (SM) susceptibility. We performed high-resolution variant discovery of rare and common genetic variants in the humanCD55gene. Association testing of these variants in over 1700 SM cases and unaffected control individuals from the malaria-endemic Ashanti Region in Ghana, West Africa, were performed on the basis of single variants, combined rare variant analyses, and reconstructed haplotypes. A total of 26 genetic variants were detected in coding and regulatory regions ofCD55Five variants were previously unknown. None of the single variants, rare variants, or haplotypes showed evidence for association with SM orP. falciparumdensity. Here, we present the first comprehensive analysis of variation in theCD55gene in the context of SM and show that genetic variants present in a Ghanaian study group appear not to influence susceptibility to the disease.
authors.affiliation: Department of Molecular Medicine, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany schuldt@bnitm.de.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Schuldt
authors.affiliation: Department of Molecular Medicine, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany.
authors.forename: Christa
authors.initials: C
authors.lastname: Ehmen
authors.affiliation: Department of Molecular Medicine, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany.
authors.forename: Juergen
authors.initials: J
authors.lastname: Sievertsen
authors.affiliation: Department of Molecular Medicine, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Evans
authors.affiliation: Infectious Disease Epidemiology Group, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany.
authors.forename: Juergen
authors.initials: J
authors.lastname: May
authors.affiliation: School of Medical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
authors.forename: Daniel
authors.initials: D
authors.lastname: Ansong
authors.affiliation: Department of Molecular Medicine, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Muntau
authors.affiliation: Department of Molecular Medicine, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany.
authors.forename: Gerd
authors.initials: G
authors.lastname: Ruge
authors.affiliation: Department of Molecular Medicine, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Timmann
authors.affiliation: School of Medical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
authors.forename: Tsiri
authors.initials: T
authors.lastname: Agbenyega
authors.affiliation: Department of Molecular Medicine, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany.
authors.forename: Rolf D
authors.initials: RD
authors.lastname: Horstmann
authors.affiliation: Department of Molecular Medicine, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Thye
mesh_terms: D018958:CD55 Antigens / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005869:Ghana; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D007223:Infant; D016778:Malaria, Falciparum / Q000235:genetics*; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D018958:CD55 Antigens
keywords: CD55; DAF; genetic association study; high-resolution screen; malaria