title: Compact diode laser source for multiphoton biological imaging.
abstract: We demonstrate a compact, pulsed diode laser source suitable for multiphoton microscopy of biological samples. The center wavelength is 976 nm, near the peak of the two-photon cross section of common fluorescent markers such as genetically encoded green and yellow fluorescent proteins. The laser repetition rate is electrically tunable between 66.67 kHz and 10 MHz, with 2.3 ps pulse duration and peak powers >1 kW. The laser components are fiber-coupled and scalable to a compact package. We demonstrate >600 μm depth penetration in brain tissue, limited by laser power.
authors.affiliation: Department of Physics, University of Colorado, 390 UCB, Boulder, CO 80309-0390, USA.
authors.forename: Robert D
authors.initials: RD
authors.lastname: Niederriter
authors.affiliation: Department of Bioengineering, University of Colorado Denver, Anschutz Medical Campus, Mail Stop 8607, 12700 East 19th Ave, Aurora, CO 80045, USA.
authors.forename: Baris N
authors.initials: BN
authors.lastname: Ozbay
authors.affiliation: Department of Bioengineering, University of Colorado Denver, Anschutz Medical Campus, Mail Stop 8607, 12700 East 19th Ave, Aurora, CO 80045, USA.
authors.forename: Gregory L
authors.initials: GL
authors.lastname: Futia
authors.affiliation: Department of Bioengineering, University of Colorado Denver, Anschutz Medical Campus, Mail Stop 8607, 12700 East 19th Ave, Aurora, CO 80045, USA.
authors.forename: Emily A
authors.initials: EA
authors.lastname: Gibson
authors.affiliation: Department of Physics, University of Colorado, 390 UCB, Boulder, CO 80309-0390, USA; Department of Electrical, Computer, and Energy Engineering, University of Colorado, 425 UCB, Boulder, CO 80309-0425, USA.
authors.forename: Juliet T
authors.initials: JT
authors.lastname: Gopinath
keywords: (140.3538) Lasers, pulsed; (140.5960) Semiconductor lasers; (180.2520) Fluorescence microscopy; (180.4315) Nonlinear microscopy