title: Comparison of various types of collagenous scaffolds applied for embryonic nerve cell culture.
abstract: The purpose of the study was to confirm whether collagen-based scaffolds using different cross-linking methods are suitable elaborate environments for embryonic nerve cell culture. Three 3D sponge-shaped porous scaffolds were composed using collagen alone, collagen with chondroitin sulphate modified by 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride, and collagen cross-linked by 2,3-dialdehyde cellulose (DAC). Embryonic nerve cells from rats were applied to the scaffolds and stained with bisbenzimide to study cell entrapment within the scaffolds. The metabolic activity of the cells cultured in the scaffolds was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The majority of cells were differentiated into neurocytes or oligodendrocytes. Collagen and collagen-chondroitin sulphate scaffolds entrapped a low number of cells. The highest cell density was found in the collagen-DAC scaffold. Moreover, in collagen-DAC scaffolds, the metabolic activity was markedly higher than in the other samples. Although all used scaffolds are suitable for the culture of embryonic nerve cells, the collagen-DAC scaffold properties are the most favorable. This scaffold entraps the highest number of cells and constitutes a favorable environment for their culture. Hence, the Col-DAC scaffold is recommended as an effective carrier for embryonic nerve cells.
authors.affiliation: Laboratory of Connective Tissue Metabolism, Department of Neuropeptides Research, Medical University of Lodz, Poland. Electronic address: drob@op.pl.
authors.forename: Jacek
authors.initials: J
authors.lastname: Drobnik
authors.affiliation: Department of Material and Commodity Sciences and Textile Metrology, Lodz University of Technology, Poland.
authors.forename: Krystyna
authors.initials: K
authors.lastname: Pietrucha
authors.affiliation: Textile Research Institute, Lodz, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Kudzin
authors.affiliation: Textile Research Institute, Lodz, Poland.
authors.forename: Krystyna
authors.initials: K
authors.lastname: Mader
authors.affiliation: Central Scientific Laboratory, Medical University of Lodz, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Szymański
authors.affiliation: Laboratory of Connective Tissue Metabolism, Department of Neuropeptides Research, Medical University of Lodz, Poland.
authors.forename: Alicja
authors.initials: A
authors.lastname: Szczepanowska
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000737:chemistry / Q000378:metabolism; D003094:Collagen / Q000737:chemistry / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000166:cytology; D005260:Female; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D009836:Oligodendroglia / Q000166:cytology / Q000502:physiology; D016062:Porosity; D011247:Pregnancy; D051381:Rats; D015203:Reproducibility of Results; D013552:Swine; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D002809:Chondroitin Sulfates; D003094:Collagen
keywords: Biomaterials; Chondroitin sulphate; Collagen; Embryonic nervous cells