title: Combining doxorubicin with a phenolic extract from flaxseed oil: Evaluation of the effect on two breast cancer cell lines.
abstract: Breast cancer is one of the most frequently diagnosed forms of cancer and different treatments are used to block its progression. However, it still represents a very common cause of death in women. Doxorubicin (Dox) is reported as an effective agent in breast cancer treatment nonetheless it induces many side‑effects. For this reason, many laboratories are engaged in understanding how it is possible to decrease the drug concentration, considering that one of the possible solutions is to use drug synergy, combining it with natural substances. Recently we showed that a phenolic extract from flaxseed (FS) oil, named PEFSO, induced on MCF‑7 cell line an increase of apoptosis with related modification of G0/G1 phase cell cycle, and the activation of signaling and pro‑oxidant pathways. In this study we present data on the combined effect of Dox and PEFSO on two different breast cancer cell lines to define the conditions to use lower doses of this chemotherapeutic agent. We report the data relating to the ability of this mixture to induce cytotoxicity and apoptosis, cell cycle modification, mitochondrial membrane depolarization and activation of extrinsic and/or intrinsic apoptotic pathway.
authors.affiliation: CROM, Istituto Nazionale Tumori 'Fondazione G. Pascale' ‑ IRCCS, Naples, Italy.
authors.forename: Eliana
authors.initials: E
authors.lastname: Guerriero
authors.affiliation: CROM, Istituto Nazionale Tumori 'Fondazione G. Pascale' ‑ IRCCS, Naples, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Sorice
authors.affiliation: CROM, Istituto Nazionale Tumori 'Fondazione G. Pascale' ‑ IRCCS, Naples, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Capone
authors.affiliation: Onco-Hematology Department, Avellino Hospital, Avellino, Italy.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Storti
authors.affiliation: Medical Informatics Service, University Hospital, Second University of Naples, Naples, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Colonna
authors.affiliation: Scientific Direction, Istituto Nazionale Tumori 'Fondazione G. Pascale' ‑ IRCCS, Naples, Italy.
authors.forename: Gennaro
authors.initials: G
authors.lastname: Ciliberto
authors.affiliation: CROM, Istituto Nazionale Tumori 'Fondazione G. Pascale' ‑ IRCCS, Naples, Italy.
authors.forename: Susan
authors.initials: S
authors.lastname: Costantini
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000008:administration & dosage*; D004357:Drug Synergism; D005260:Female; D006801:Humans; D008043:Linseed Oil / Q000008:administration & dosage*; D061986:MCF-7 Cells; D010936:Plant Extracts / Q000008:administration & dosage*; D016133:Polymerase Chain Reaction
chemical_list: D010936:Plant Extracts; D008043:Linseed Oil; D004317:Doxorubicin