title: Microsporidial Spores in Fecal Samples of Some Domesticated Animals Living in Giza, Egypt.
abstract: BACKGROUND
  The aim of the present work was to investigate the prevalence and species of intestinal microsporidiosis among animals in Giza, Egypt.
  
  
  METHODS
  A total of 869 animal fecal samples were collected from domesticated animals (dogs, cats, rabbits, cattle, buffaloes, sheep, goats, donkeys and pigs) living in Giza, Egypt. Spores of microsporidia were concentrated from collected samples by centrifugation and finally stained with modified trichrome (MT) stain to detect microsporidial spores. Microsporidial spores in microscopically-positive samples were molecularly confirmed and identified using species-specific primers.
  
  
  RESULTS
  Spores of microsporidia were microscopically detected in 17.0% of the examined animal fecal samples. The highest and lowest rates of infection with intestinal microsporidia were recorded in dogs (33.3%) and buffaloes (6.9%), respectively. Molecularly, the obtained microsporidial spores were classified asEnterocytozoon bieneusiandE. intestinalis. Dual infection with both identified species was observed in fecal samples from buffalo, rabbit, goat, cat, pig and dog.
  
  
  CONCLUSIONS
  Domestic animals may play a role in dissemination of intestinal microsporidiosis in the environment. Examined animals were infected withE. bieneusiin a higher percentage thanE. intestinalis.
authors.affiliation: Dept. of Water Pollution Research, National Research Centre, 12622 Dokki, Giza, Egypt.
authors.forename: Ahmad Z
authors.initials: AZ
authors.lastname: Al-Herrawy
authors.affiliation: Dept. of Water Pollution Research, National Research Centre, 12622 Dokki, Giza, Egypt.
authors.forename: Mahmoud A
authors.initials: MA
authors.lastname: Gad
keywords: Domesticated animals; Fecal samples; Intestinal microsporidia; Modified trichrome; PCR