title: The short- and long-term proteomic effects of sleep deprivation on the cortical and thalamic synapses.
abstract: Acute total sleep deprivation (SD) impairs memory consolidation, attention, working memory and perception. Structural, electrophysiological and molecular experimental approaches provided evidences for the involvement of sleep in synaptic functions. Despite the wide scientific interest on the effects of sleep on the synapse, there is a lack of systematic investigation of sleep-related changes in the synaptic proteome. We isolated parietal cortical and thalamic synaptosomes of rats after 8h of total SD by gentle handling and 16h after the end of deprivation to investigate the short- and longer-term effects of SD on the synaptic proteome, respectively. The SD efficiency was verified by electrophysiology. Protein abundance alterations of the synaptosomes were analyzed by fluorescent two-dimensional differential gel electrophoresis and by tandem mass spectrometry. As several altered proteins were found to be involved in synaptic strength regulation, our data can support the synaptic homeostasis hypothesis function of sleep and highlight the long-term influence of SD after the recovery sleep period, mostly on cortical synapses. Furthermore, the large-scale and brain area-specific protein network change in the synapses may support both ideas of sleep-related synaptogenesis and molecular maintenance and reorganization in normal rat brain.
authors.affiliation: Laboratory of Proteomics, Institute of Biology, Eötvös Loránd University, Budapest H-1117, Hungary.
authors.forename: Attila
authors.initials: A
authors.lastname: Simor
authors.affiliation: Laboratory of Proteomics, Institute of Biology, Eötvös Loránd University, Budapest H-1117, Hungary; MTA-ELTE NAP B Neuroimmunology Research Group, Department of Biochemistry, Institute of Biology, Eötvös Loránd University, Budapest H-1117, Hungary.
authors.forename: Balázs András
authors.initials: BA
authors.lastname: Györffy
authors.affiliation: Laboratory of Proteomics, Institute of Biology, Eötvös Loránd University, Budapest H-1117, Hungary; MTA-TTK NAP B MS Neuroproteomics Research Group, Hungarian Academy of Sciences, Budapest H-1117, Hungary.
authors.forename: Péter
authors.initials: P
authors.lastname: Gulyássy
authors.affiliation: Laboratory of Proteomics, Institute of Biology, Eötvös Loránd University, Budapest H-1117, Hungary; MTA-ELTE NAP B Laboratory of Molecular and Systems Neurobiology, Institute of Biology, Hungarian Academy of Sciences and Eötvös Loránd University, Budapest H-1117, Hungary.
authors.forename: Katalin
authors.initials: K
authors.lastname: Völgyi
authors.affiliation: Laboratory of Proteomics, Institute of Biology, Eötvös Loránd University, Budapest H-1117, Hungary; MTA-TTK NAP B MS Neuroproteomics Research Group, Hungarian Academy of Sciences, Budapest H-1117, Hungary.
authors.forename: Vilmos
authors.initials: V
authors.lastname: Tóth
authors.affiliation: Laboratory of Proteomics, Institute of Biology, Eötvös Loránd University, Budapest H-1117, Hungary.
authors.forename: Mihail Ivilinov
authors.initials: MI
authors.lastname: Todorov
authors.affiliation: Department of Anatomy, Cell and Developmental Biology, Institute of Biology, Eötvös Loránd University, Budapest H-1117, Hungary.
authors.forename: Viktor
authors.initials: V
authors.lastname: Kis
authors.affiliation: MTA-ELTE-NAP B Opto-Neuropharmacology Group, Hungarian Academy of Sciences and Eötvös Loránd University, Budapest H-1117, Hungary.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Borhegyi
authors.affiliation: Institute of Medical Chemistry, University of Szeged, Szeged H-6720, Hungary.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Szabó
authors.affiliation: Institute of Medical Chemistry, University of Szeged, Szeged H-6720, Hungary.
authors.forename: Tamás
authors.initials: T
authors.lastname: Janáky
authors.affiliation: MTA-TTK NAP B MS Neuroproteomics Research Group, Hungarian Academy of Sciences, Budapest H-1117, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Drahos
authors.affiliation: Laboratory of Proteomics, Institute of Biology, Eötvös Loránd University, Budapest H-1117, Hungary; MTA-TTK NAP B MS Neuroproteomics Research Group, Hungarian Academy of Sciences, Budapest H-1117, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Juhász
authors.affiliation: Laboratory of Proteomics, Institute of Biology, Eötvös Loránd University, Budapest H-1117, Hungary; Department of Physiology and Neurobiology, Eötvös Loránd University, Budapest H-1117, Hungary. Electronic address: kakekesi@ttk.elte.hu.
authors.forename: Katalin Adrienna
authors.initials: KA
authors.lastname: Kékesi
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012892:Sleep Deprivation / Q000378:metabolism* / Q000473:pathology; D013569:Synapses / Q000378:metabolism* / Q000648:ultrastructure; D013788:Thalamus / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D020543:Proteome
keywords: 2D-DIGE proteomics; Parietal cortex; Sleep deprivation; Synaptic homeostasis hypothesis; Synaptosome; Thalamus