title: Schinus terebinthifolius countercurrent chromatography (Part III): Method transfer from small countercurrent chromatography column to preparative centrifugal partition chromatography ones as a part of method development.
abstract: Countercurrent chromatography (CCC) and centrifugal partition chromatography (CPC) are support free liquid-liquid chromatography techniques sharing the same basic principles and features. Method transfer has previously been demonstrated for both techniques but never from one to another. This study aimed to show such a feasibility using fractionation of Schinus terebinthifolius berries dichloromethane extract as a case study. Heptane - ethyl acetate - methanol -water (6:1:6:1, v/v/v/v) was used as solvent system with masticadienonic and 3β-masticadienolic acids as target compounds. The optimized separation methodology previously described in Part I and II, was scaled up from an analytical hydrodynamic CCC column (17.4mL) to preparative hydrostatic CPC instruments (250mL and 303mL) as a part of method development. Flow-rate and sample loading were further optimized on CPC. Mobile phase linear velocity is suggested as a transfer invariant parameter if the CPC column contains sufficient number of partition cells.
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Pesquisas de Produtos Naturais, Rio de Janeiro, Brazil. Electronic address: fncosta@nppn.ufrj.br.
authors.forename: Fernanda
authors.initials: F
authors.lastname: das Neves Costa
authors.affiliation: UMR CNRS 7312, Institut de Chimie Moléculaire de Reims, Université de Reims Champagne-Ardenne, Reims, France.
authors.forename: Jane
authors.initials: J
authors.lastname: Hubert
authors.affiliation: UMR CNRS 7312, Institut de Chimie Moléculaire de Reims, Université de Reims Champagne-Ardenne, Reims, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Borie
authors.affiliation: UMR CNRS 7312, Institut de Chimie Moléculaire de Reims, Université de Reims Champagne-Ardenne, Reims, France.
authors.forename: Alexis
authors.initials: A
authors.lastname: Kotland
authors.affiliation: Advanced Bioprocessing Centre, Institute of Environment, Health & Societies, CEDPS, Brunel University London, Middlesex, UB8 3PH, UK.
authors.forename: Peter
authors.initials: P
authors.lastname: Hewitson
authors.affiliation: Advanced Bioprocessing Centre, Institute of Environment, Health & Societies, CEDPS, Brunel University London, Middlesex, UB8 3PH, UK.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Ignatova
authors.affiliation: UMR CNRS 7312, Institut de Chimie Moléculaire de Reims, Université de Reims Champagne-Ardenne, Reims, France.
authors.forename: Jean-Hugues
authors.initials: JH
authors.lastname: Renault
mesh_terms: D027926:Anacardiaceae / Q000737:chemistry*; D005591:Chemical Fractionation; D002623:Chemistry Techniques, Analytical / Q000379:methods* / Q000592:standards; D002853:Chromatography, Liquid; D003377:Countercurrent Distribution; D005638:Fruit / Q000737:chemistry; D000432:Methanol / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D012997:Solvents / Q000737:chemistry; D014315:Triterpenes / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D010936:Plant Extracts; D012997:Solvents; D014315:Triterpenes; C459621:masticadienolic acid; D014867:Water; D000432:Methanol
keywords: Centrifugal partition chromatography; Countercurrent chromatography; Method transfer; Schinus terebinthifolius