title: Hypermethylation of antisense long noncoding RNAs in acute lymphoblastic leukemia.
abstract: Long noncoding RNAs serve critical regulatory functions highly specific for a tissue and its developmental stage. Antisense long ncRNA (AS-lncRNA) methylation changes in acute lymphoblastic leukemia (ALL) versus normal pre-B-cell lymphoblasts were evaluated to identify potential differential methylation in this group of genes.
  
  
  
  The methylome of ALL and normal lymphoblasts was examined by the methylated CpG island recovery assay followed by NGS.
  
  
  
  The potential effect of trans regulation by AS-lncRNA through DNA/RNA binding is significant as sequence alignment analysis of the 25 most differentially methylated AS-lncRNAs revealed 368 genes containing highly similar sequences with a median nucleotide identity of 90.8% and binding span of 122 base pairs. Regulation of biological processes and anatomical structure development were over represented. ALL classification schemes based on AS-lncRNA methylation can provide new insights into its pathogenesis and treatment.
authors.affiliation: Department of Pathology & Anatomical Sciences, University of Missouri-Columbia, Columbia, MO 65212, USA.|MU Informatics Institute, University of Missouri-Columbia, Columbia, MO 65212, USA.
authors.forename: Gerald
authors.initials: G
authors.lastname: Arthur
authors.affiliation: Hematology/Oncology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Md
authors.initials: M
authors.lastname: Almamun
authors.affiliation: Department of Pathology & Anatomical Sciences, University of Missouri-Columbia, Columbia, MO 65212, USA.
authors.forename: Kristen
authors.initials: K
authors.lastname: Taylor
mesh_terms: D001402:B-Lymphocytes / Q000378:metabolism; D016022:Case-Control Studies; D018899:CpG Islands; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D016372:RNA, Antisense / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000235:genetics*
chemical_list: D016372:RNA, Antisense; D062085:RNA, Long Noncoding
keywords: DNA methylation; epigenetic regulations; lncRNA