title: Overexpression of wheat ferritin gene TaFER-5B enhances tolerance to heat stress and other abiotic stresses associated with the ROS scavenging.
abstract: The yield of wheat (Triticum aestivum L.), an important crop, is adversely affected by heat stress in many regions of the world. However, the molecular mechanisms underlying thermotolerance are largely unknown.
  
  
  
  A novel ferritin gene, TaFER, was identified from our previous heat stress-responsive transcriptome analysis of a heat-tolerant wheat cultivar (TAM107). TaFER was mapped to chromosome 5B and named TaFER-5B. Expression pattern analysis revealed that TaFER-5B was induced by heat, polyethylene glycol (PEG), H2O2and Fe-ethylenediaminedi(o-hydroxyphenylacetic) acid (Fe-EDDHA). To confirm the function of TaFER-5B in wheat, TaFER-5B was transformed into the wheat cultivar Jimai5265 (JM5265), and the transgenic plants exhibited enhanced thermotolerance. To examine whether the function of ferritin from mono- and dico-species is conserved, TaFER-5B was transformed into Arabidopsis, and overexpression of TaFER-5B functionally complemented the heat stress-sensitive phenotype of a ferritin-lacking mutant of Arabidopsis. Moreover, TaFER-5B is essential for protecting cells against heat stress associated with protecting cells against ROS. In addition, TaFER-5B overexpression also enhanced drought, oxidative and excess iron stress tolerance associated with the ROS scavenging. Finally, TaFER-5B transgenic Arabidopsis and wheat plants exhibited improved leaf iron content.
  
  
  
  Our results suggest that TaFER-5B plays an important role in enhancing tolerance to heat stress and other abiotic stresses associated with the ROS scavenging.
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, NO.2 Yuanmingyuan Xi Road, Haidian District, Beijing, 100193, China.
authors.forename: Xinshan
authors.initials: X
authors.lastname: Zang
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, NO.2 Yuanmingyuan Xi Road, Haidian District, Beijing, 100193, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Geng
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, NO.2 Yuanmingyuan Xi Road, Haidian District, Beijing, 100193, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, NO.2 Yuanmingyuan Xi Road, Haidian District, Beijing, 100193, China.
authors.forename: Zhenshan
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, NO.2 Yuanmingyuan Xi Road, Haidian District, Beijing, 100193, China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, NO.2 Yuanmingyuan Xi Road, Haidian District, Beijing, 100193, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, NO.2 Yuanmingyuan Xi Road, Haidian District, Beijing, 100193, China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Tian
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, NO.2 Yuanmingyuan Xi Road, Haidian District, Beijing, 100193, China.
authors.forename: Zhongfu
authors.initials: Z
authors.lastname: Ni
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, NO.2 Yuanmingyuan Xi Road, Haidian District, Beijing, 100193, China.
authors.forename: Yingyin
authors.initials: Y
authors.lastname: Yao
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, NO.2 Yuanmingyuan Xi Road, Haidian District, Beijing, 100193, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Xin
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, NO.2 Yuanmingyuan Xi Road, Haidian District, Beijing, 100193, China.
authors.forename: Zhaorong
authors.initials: Z
authors.lastname: Hu
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, NO.2 Yuanmingyuan Xi Road, Haidian District, Beijing, 100193, China.
authors.forename: Qixin
authors.initials: Q
authors.lastname: Sun
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, NO.2 Yuanmingyuan Xi Road, Haidian District, Beijing, 100193, China. penghuiru@cau.edu.cn.
authors.forename: Huiru
authors.initials: H
authors.lastname: Peng
mesh_terms: D055864:Droughts; D005293:Ferritins / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D006358:Hot Temperature; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013312:Stress, Physiological; D014908:Triticum / Q000235:genetics / Q000502:physiology*
chemical_list: D010940:Plant Proteins; D017382:Reactive Oxygen Species; D005293:Ferritins
keywords: Abiotic stress; Arabidopsis; Ferritin-lacking mutant; Heat stress; TaFER-5B; Wheat