title: Molecularly Imprinted Solid Phase Extraction using Bismethacryloyl-β-cyclodextrin and Methacrylic Acid as Double Functional Monomers for Selective Analysis of Glycyrrhizic Acid in Aqueous Media.
abstract: In this work, a new molecularly imprinted solid phase extraction protocol was developed for the selective extraction and purification of glycyrrhizic acid from liquorice roots in aqueous media. The molecularly imprinted polymers (MIPs) for glycyrrhizic acid were prepared by using bismethacryloyl-β-cyclodextrin and methacrylic acid as double functional monomers and characterized by Fourier transform infrared spectroscopy, scanning electron microscope, thermo gravimetric analysis, nitrogen adsorption and elemental analysis. In aqueous media, the adsorption properties of MIPs including adsorption kinetics, adsorption isotherms and selectivity adsorption were investigated. The characterization of imprinted polymers indicated that the prepared MIPs had good stability and many cavity structures. The results of adsorption experiments illustrated the MIPs had high adsorption capacity of glycyrrhizic acid (69.3 mg g-1) with the imprinting factor 3.77, and it took ~5 min to get adsorption equilibrium. The MIPs could be used as an solid phase extraction sorbent absorbent for enrichment and purification of glycyrrhizic acid from the crude extraction of licorice roots, and the results showed promising practical value.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, P.R. China.|Department of Pharmacy, Hospital of Stomatology Xi'an Jiaotong University, Xi'an 710004, P.R. China.
authors.forename: Weili
authors.initials: W
authors.lastname: Tang
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Du
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, P.R. China.
authors.forename: Pengqi
authors.initials: P
authors.lastname: Guo
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, P.R. China.|Department of Pharmacy, Xi'an First Hospital, Xi'an 710002, P.R. China.
authors.forename: Ningli
authors.initials: N
authors.lastname: Wu
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, P.R. China.|Department of Pharmacy, Tianjin Huanhu Hospital, Tianjin 300060, P.R. China.
authors.forename: Kangli
authors.initials: K
authors.lastname: Du
authors.affiliation: Lab of Chemical, Shaanxi Institute for Food and Drug Control, Xi'an 710065, P.R. China.
authors.forename: Changgen
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, P.R. China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Luo
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, P.R. China.
authors.forename: Ruimiao
authors.initials: R
authors.lastname: Chang
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, P.R. China.
authors.forename: Aiguo
authors.initials: A
authors.lastname: Zeng
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, P.R. China.
authors.forename: Wanghui
authors.initials: W
authors.lastname: Jing
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, P.R. China.
authors.forename: Chun
authors.initials: C
authors.lastname: Chang
authors.affiliation: Lab of Chemical, Shaanxi Institute for Food and Drug Control, Xi'an 710065, P.R. China.
authors.forename: Ji
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, P.R. China fuqiang@mail.xjtu.edu.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
mesh_terms: D019695:Glycyrrhizic Acid / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D057230:Limit of Detection; D016014:Linear Models; D008689:Methacrylates / Q000737:chemistry*; D054802:Molecular Imprinting / Q000379:methods*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D008689:Methacrylates; D047392:beta-Cyclodextrins; C008384:methacrylic acid; D019695:Glycyrrhizic Acid