title: Altered oxidative stress and carbohydrate metabolism in canine mammary tumors.
abstract: OBJECTIVE
  Mammary tumors are the most prevalent type of neoplasms in canines. Even though cancer induced metabolic alterations are well established, the clinical data describing the metabolic profiles of animal tumors is not available. Hence, our present investigation was carried out with the aim of studying changes in carbohydrate metabolism along with the level of oxidative stress in canine mammary tumors.
  
  
  METHODS
  Fresh mammary tumor tissues along with the adjacent healthy tissues were collected from the college surgical ward. The levels of thiobarbituric acid reactive substances (TBARS), glutathione, protein, hexose, hexokinase, glucose-6-phosphatase, fructose-1, 6-bisphosphatase, and glucose-6-phosphate dehydrogenase (G6PD) were analyzed in all the tissues. The results were analyzed statistically.
  
  
  RESULTS
  More than two-fold increase in TBARS and three-fold increase in glutathione levels were observed in neoplastic tissues. Hexokinase activity and hexose concentration (175%) was found to be increased, whereas glucose-6-phosphatase (33%), fructose-1, 6-bisphosphatase (42%), and G6PD (5 fold) activities were reduced in tumor mass compared to control.
  
  
  CONCLUSIONS
  Finally, it was revealed that lipid peroxidation was increased with differentially altered carbohydrate metabolism in canine mammary tumors.
authors.affiliation: Department of Veterinary Biochemistry, College of Veterinary Science, Tirupati, Andhra Pradesh, India.
authors.forename: K
authors.initials: K
authors.lastname: Jayasri
authors.affiliation: Department of Veterinary Biochemistry, College of Veterinary Science, Tirupati, Andhra Pradesh, India.
authors.forename: K
authors.initials: K
authors.lastname: Padmaja
authors.affiliation: Department of Surgery and Radiology, College of Veterinary Science, Tirupati, Andhra Pradesh, India.
authors.forename: M
authors.initials: M
authors.lastname: Saibaba
keywords: 6-bisphosphatase; canine mammary tumor; fructose-1; glucose-6-phosphatase; glucose-6-phosphate dehydrogenase; hexokinase; thiobarbituric reactive substances