title: FRET-based nanobiosensor for detection of scopolamine in hairy root extraction of Atropa belladonna.
abstract: A simple, sensitive, selective, and rapid optical nanobiosensor based on FRET was designed to detect tropane alkaloids as anti-cholinergic agents in natural and transgenic hairy roots extracts of Atropa belladonna. To achieve that, conjugation of tioglycolyic acid capped cadmium telluride quantum Dots, M2muscarinic receptor (Cd/Te QDs-M2R) and conjugation of scopolamine-rhodamine123 (Sc-Rho123) were performed. More specifically, proportional amounts of M2muscarinic receptor and quantum dots (QDs) were conjugated while scopolamine (as a tropane alkaloid) and rhodamine123 were also combined and these moieties functioned as donor and acceptor pairs, respectively. The system response was linear over the range of 0.01-4µmolL-1of scopolamine hydrochloride concentration with a detection limit of 0.001µmolL-1. The developed nanobiosensor was successfully used for in vitro recognition of scopolamine as an anti-cholinergic agent in the investigated plant extracts. In addition, Agrobacterium rhizogenesis mediated gene transfer technique was employed to generate hairy roots and to enhance the production of tropane alkaloids in the studied medicinal plant.
authors.affiliation: Department of Biotechnology, College of Agriculture, Bu-Ali Sina University, Hamedan, Iran; Research Pharmaceutical Center, School of pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran. Electronic address: f.bagheri2016@yahoo.com.
authors.forename: Fereshte
authors.initials: F
authors.lastname: Bagheri
authors.affiliation: Department of Biotechnology, College of Agriculture, Bu-Ali Sina University, Hamedan, Iran. Electronic address: khpiri@gmail.com.
authors.forename: Khosro
authors.initials: K
authors.lastname: Piri
authors.affiliation: Research and Development Department of Nanozino, Tehran, Iran. Electronic address: mohsenifar_a@yahoo.com.
authors.forename: Afshine
authors.initials: A
authors.lastname: Mohsenifar
authors.affiliation: Department of Chemistry, Bu-Ali Sina University, Hamedan, Iran.
authors.forename: Smaiil
authors.initials: S
authors.lastname: Ghaderi
mesh_terms: D060054:Agrobacterium / Q000378:metabolism; D001532:Atropa belladonna / Q000737:chemistry* / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D055620:Optical Phenomena; D010084:Oxidation-Reduction; D018515:Plant Leaves / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry* / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism; D045663:Quantum Dots / Q000737:chemistry; D012601:Scopolamine / Q000032:analysis* / Q000737:chemistry; D013997:Time Factors
chemical_list: D012601:Scopolamine
keywords: Cd/Te quantum dots; FRET; Hairy roots; M(2) muscarinic receptor; Nanobiosensor; Tropane alkaloids