title: 2'-O-Methyl-5-hydroxymethylcytidine: A Second Oxidative Derivative of 5-Methylcytidine in RNA.
abstract: 5-Hydroxymethylcytidine (hm5C) was recently identified as a direct metabolite of m5C in RNA. We investigated the stability of hm5C in human cells using bio-isotopologues and LC-MS/HRMS. This has led to the discovery of a second oxidative metabolite of m5C in RNA, namely 2'-O-methyl-5-hydroxymethylcytidine (hm5Cm). Subsequent quantitative analysis of total RNA from higher organisms revealed varying levels and TET-independent formation of this new RNA modification.
authors.affiliation: Department of Chemistry, University of Cambridge , Lensfield Road, Cambridge CB2 1EW, United Kingdom.
authors.forename: Sabrina M
authors.initials: SM
authors.lastname: Huber
authors.affiliation: Department of Chemistry, University of Cambridge , Lensfield Road, Cambridge CB2 1EW, United Kingdom.
authors.forename: Pieter
authors.initials: P
authors.lastname: van Delft
authors.affiliation: Department of Chemistry, University of Cambridge , Lensfield Road, Cambridge CB2 1EW, United Kingdom.
authors.forename: Arun
authors.initials: A
authors.lastname: Tanpure
authors.affiliation: Wellcome Trust/Cancer Research UK Gurdon Institute , Tennis Court Road, Cambridge CB2 1QN, United Kingdom.
authors.forename: Eric A
authors.initials: EA
authors.lastname: Miska
authors.affiliation: Department of Chemistry, University of Cambridge , Lensfield Road, Cambridge CB2 1EW, United Kingdom.
authors.forename: Shankar
authors.identifier: 0000-0002-0281-5815
authors.initials: S
authors.lastname: Balasubramanian
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002853:Chromatography, Liquid; D003562:Cytidine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D015394:Molecular Structure; D010084:Oxidation-Reduction; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: C000627341:2'-O-methyl-5-hydroxymethylcytidine; D003562:Cytidine; D012313:RNA; C016568:5-methylcytidine