title: Frankia discariae sp. nov.: an infective and effective microsymbiont isolated from the root nodule of Discaria trinervis.
abstract: Strain BCU110501Twas the first isolate reported to fulfill Koch's postulates by inducing effective nodules on its host plant of origin Discaria trinervis (Rhalmnaceae). Based on 16S rRNA gene sequence similarities, the strain was found to be most closely related to the type strain of Frankia elaeagni DSM 46783T(98.6%) followed by F. alni DSM 45986T(98.2%), F. casuarinae DSM 45818T(97.8%) and F. inefficacies DSM 45817T(97.8%). Digital DNA:DNA hybridizations (dDDH) between strain BCU110501Tand the type strains of other Frankia species were clearly below the cutoff point of 70%. The G+C content of DNA is 72.36%. The cell wall of strain BCU110501Tcontained meso-diaminopimelic acid and the cell sugars were galactose, glucose, mannose, xylose and ribose. Polar lipids were phosphatidylinositol (PI), diphosphatidylglycerol (DPG), glycophospholipid (GPL1-3), phosphatidylglycerol (PG) and an unknown lipid (L). The major fatty acids of strain BCU110501Tconsisted of iso-C16:0, C17:1 w8c and C16:0. Major menaquinones were MK9 (H4), MK9 (H6) and MK9 (H2). Based on these analyses, strain BCU110501T(=DSM 46785T=CECT 9042T) should be classified as the type strain of a novel Frankia species, for which the name Frankia discariae sp. nov. is proposed.
authors.affiliation: Laboratoire Microorganismes et Biomolécules Actives, Université Tunis El Manar (FST) & Université de Carthage (INSAT), 2092, Tunis, Tunisia.|School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne, NE1 7RU, UK.
authors.forename: Imen
authors.initials: I
authors.lastname: Nouioui
authors.affiliation: School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne, NE1 7RU, UK.
authors.forename: Maria
authors.initials: M
authors.lastname: Del Carmen Montero-Calasanz
authors.affiliation: Laboratoire Microorganismes et Biomolécules Actives, Université Tunis El Manar (FST) & Université de Carthage (INSAT), 2092, Tunis, Tunisia.
authors.forename: Faten
authors.initials: F
authors.lastname: Ghodhbane-Gtari
authors.affiliation: HZI-Helmholtz Centre for Infection Research, Inhoffenstraße7, 38124, Brunswick, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: Rohde
authors.affiliation: Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, 289 Rudman Hall, 46 college Road, Durham, NH, 03824-2617, USA.
authors.forename: Louis S
authors.initials: LS
authors.lastname: Tisa
authors.affiliation: School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne, NE1 7RU, UK.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Klenk
authors.affiliation: Laboratoire Microorganismes et Biomolécules Actives, Université Tunis El Manar (FST) & Université de Carthage (INSAT), 2092, Tunis, Tunisia. maher.gtari@fst.rnu.tn.
authors.forename: Maher
authors.identifier: http://orcid.org/0000-0001-6946-6907
authors.initials: M
authors.lastname: Gtari
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition / Q000235:genetics; D001483:Base Sequence; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D040161:Frankia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D027823:Rhamnaceae / Q000382:microbiology*; D053204:Root Nodules, Plant / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013559:Symbiosis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid
keywords: Chemotaxonomy; Discaria; Frankia; Symbiosis; Type strain