title: Mass Spectrometry: A Technique of Many Faces.
abstract: Protein complexes form the critical foundation for a wide range of biological process, however understanding the intricate details of their activities is often challenging. In this review we describe how mass spectrometry plays a key role in the analysis of protein assemblies and the cellular pathways which they are involved in. Specifically, we discuss how the versatility of mass spectrometric approaches provides unprecedented information on multiple levels. We demonstrate this on the ubiquitin-proteasome proteolytic pathway, a process that is responsible for protein turnover. We follow the various steps of this degradation route and illustrate the different mass spectrometry workflows that were applied for elucidating molecular information. Overall, this review aims to stimulate the integrated use of multiple mass spectrometry approaches for analyzing complex biological systems.
authors.affiliation: Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot 7610001, Israel.
authors.forename: Maya A
authors.initials: MA
authors.lastname: Olshina
authors.affiliation: Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot 7610001, Israel.
authors.forename: Michal
authors.initials: M
authors.lastname: Sharon
mesh_terms: D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D054875:Ubiquitination
chemical_list: D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease