title: The Pseudouridine Synthase RPUSD4 Is an Essential Component of Mitochondrial RNA Granules.
abstract: Mitochondrial gene expression is a fundamental process that is largely dependent on nuclear-encoded proteins. Several steps of mitochondrial RNA processing and maturation, including RNA post-transcriptional modification, appear to be spatially organized into distinct foci, which we have previously termed mitochondrial RNA granules (MRGs). Although an increasing number of proteins have been localized to MRGs, a comprehensive analysis of the proteome of these structures is still lacking. Here, we have applied a microscopy-based approach that has allowed us to identify novel components of the MRG proteome. Among these, we have focused our attention on RPUSD4, an uncharacterized mitochondrial putative pseudouridine synthase. We show that RPUSD4 depletion leads to a severe reduction of the steady-state level of the 16S mitochondrial (mt) rRNA with defects in the biogenesis of the mitoribosome large subunit and consequently in mitochondrial translation. We report that RPUSD4 binds 16S mt-rRNA, mt-tRNAMet, and mt-tRNAPhe, and we demonstrate that it is responsible for pseudouridylation of the latter. These data provide new insights into the relevance of RNA pseudouridylation in mitochondrial gene expression.
authors.affiliation: From the Department of Cell Biology, University of Geneva, 30 quai Ernest-Ansermet, 1211 Genève 4, Switzerland.
authors.forename: Sofia
authors.initials: S
authors.lastname: Zaganelli
authors.affiliation: Medical Research Council Mitochondrial Biology Unit, Hills Road, Cambridge CB2 0XY, United Kingdom.|Graduate Program in Areas of Basic and Applied Biology (GABBA), University of Porto, Porto 4200-135, Portugal.
authors.forename: Pedro
authors.initials: P
authors.lastname: Rebelo-Guiomar
authors.affiliation: From the Department of Cell Biology, University of Geneva, 30 quai Ernest-Ansermet, 1211 Genève 4, Switzerland.
authors.forename: Kinsey
authors.initials: K
authors.lastname: Maundrell
authors.affiliation: Wellcome Trust Centre for Mitochondrial Research, Institute of Cell and Molecular Biosciences, The Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne, NE2 4HH, United Kingdom, and.
authors.forename: Agata
authors.initials: A
authors.lastname: Rozanska
authors.affiliation: From the Department of Cell Biology, University of Geneva, 30 quai Ernest-Ansermet, 1211 Genève 4, Switzerland.
authors.forename: Sandra
authors.initials: S
authors.lastname: Pierredon
authors.affiliation: Medical Research Council Mitochondrial Biology Unit, Hills Road, Cambridge CB2 0XY, United Kingdom.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Powell
authors.affiliation: From the Department of Cell Biology, University of Geneva, 30 quai Ernest-Ansermet, 1211 Genève 4, Switzerland.
authors.forename: Alexis A
authors.identifier: 0000-0002-1721-8394
authors.initials: AA
authors.lastname: Jourdain
authors.affiliation: Institute of Genetics and Genomics of Geneva, Université de Genève, 1211 Genève 4, Switzerland.
authors.forename: Nicolas
authors.identifier: 0000-0003-2640-636X
authors.initials: N
authors.lastname: Hulo
authors.affiliation: Wellcome Trust Centre for Mitochondrial Research, Institute of Cell and Molecular Biosciences, The Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne, NE2 4HH, United Kingdom, and.
authors.forename: Robert N
authors.identifier: 0000-0002-0587-9937
authors.initials: RN
authors.lastname: Lightowlers
authors.affiliation: Wellcome Trust Centre for Mitochondrial Research, Institute of Cell and Molecular Biosciences, The Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne, NE2 4HH, United Kingdom, and.
authors.forename: Zofia M
authors.identifier: 0000-0002-0045-3152
authors.initials: ZM
authors.lastname: Chrzanowska-Lightowlers
authors.affiliation: Medical Research Council Mitochondrial Biology Unit, Hills Road, Cambridge CB2 0XY, United Kingdom.
authors.forename: Michal
authors.identifier: 0000-0001-8242-1420
authors.initials: M
authors.lastname: Minczuk
authors.affiliation: From the Department of Cell Biology, University of Geneva, 30 quai Ernest-Ansermet, 1211 Genève 4, Switzerland, jean-claude.martinou@unige.ch.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Martinou
mesh_terms: D002460:Cell Line; D006801:Humans; D019751:Intramolecular Transferases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism; D021381:Protein Transport; D012313:RNA / Q000378:metabolism*; D034622:RNA Interference; D000077278:RNA, Mitochondrial; D012336:RNA, Ribosomal, 16S / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D012358:RNA, Transfer, Met / Q000378:metabolism; D012360:RNA, Transfer, Phe / Q000378:metabolism
chemical_list: D024101:Mitochondrial Proteins; D000077278:RNA, Mitochondrial; D012336:RNA, Ribosomal, 16S; D034741:RNA, Small Interfering; D012358:RNA, Transfer, Met; D012360:RNA, Transfer, Phe; D012313:RNA; D019751:Intramolecular Transferases; C096995:pseudouridine synthases
keywords: OXPHOS; RNA; RNA modification; RNA-binding protein; RPUSD; mitochondria; mitoribosome biogenesis; pseudouridine; ribosome