title: The 1910HK/RR two-component system is essential for the virulence of Streptococcus suis serotype 2.
abstract: Streptococcus suis serotype 2 is a major zoonotic pathogen, and the two-component system plays an important role in bacterial pathogenesis. The present study targeted the 1910HK/RR two-component system of S. suis 2. A 1910HK/RR deletion mutant (Δ1910HK/RR) and the corresponding complementation strain (CΔ1910HK/RR) were constructed in S. suis 2 strain 05ZYH33. 1910HK/RR deletion had no effect on S. suis 2 growth, but significantly inhibited the adherence and invasion of S. suis 2 to HEp-2 cells. Analysis of the role of 1910HK/RR in murine and pig infection models demonstrated that 1910HK/RR played a distinct role in the virulence of S. suis 2. In addition, deletion of 1910HK/RR significantly impaired the survival of 05ZYH33 in human blood. These data provided important insights into the pathogenesis of S. suis 2.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, China.
authors.forename: Fangyan
authors.initials: F
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Tan
authors.affiliation: Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, China.
authors.forename: Zewen
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, China.
authors.forename: Keli
authors.initials: K
authors.lastname: Yang
authors.affiliation: Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, China.
authors.forename: Danna
authors.initials: D
authors.lastname: Zhou
authors.affiliation: Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, China.
authors.forename: Zhengying
authors.initials: Z
authors.lastname: Duan
authors.affiliation: Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Huanchun
authors.initials: H
authors.lastname: Chen
authors.affiliation: Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, China.
authors.forename: Yongxiang
authors.initials: Y
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: beiwc@mail.hzau.edu.cn.
authors.forename: Weicheng
authors.initials: W
authors.lastname: Bei
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000235:genetics; D002460:Cell Line; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D055786:Gene Knockout Techniques; D023061:Gene Order; D005816:Genetic Complementation Test; D056426:Genetic Loci; D016680:Genome, Bacterial; D044404:Genomic Islands; D006801:Humans; D051379:Mice; D050296:Microbial Viability; D017384:Sequence Deletion; D065288:Serogroup; D013290:Streptococcal Infections / Q000382:microbiology* / Q000401:mortality / Q000473:pathology; D017011:Streptococcus suis / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity*; D013552:Swine; D014774:Virulence / Q000235:genetics
keywords: 1910HK/RR; Streptococcus suis; Two-component system; Virulence