title: Low temperature MBBR nitrification: Microbiome analysis.
abstract: This study aims to investigate post carbon removal moving bed biofilm reactor (MBBR) nitrification through the transition from 20 °C to 1 °C and during through long term operation at 1 °C. Four pilot nitrifying MBBR reactors were operated at various ammonia loading rates to elucidate the temperature effects on ammonia removal rates, cell viability and bacterial communities. The transition from 20 °C to 1 °C and during long term operation at 1 °C were modeled using Arrhenius temperature correction coefficients. Specifically, the steady state removal rates at 1 °C on average were 22.8% of the maximum ammonia removal rate at 20 °C, which corresponds to an Arrhenius temperature correction of 1.086 during steady operation at 1 °C. The microbial communities of the nitrifying MBBR biofilm were shown to be significantly more diverse at 20 °C as compared to 1 °C operation. Although less diverse at 1 °C, 2000 species of bacteria were identified in the nitrifying biofilm during operation at this low temperature. Nitrosomonads were shown to be the dominant ammonia oxidizing bacteria (AOB) and Nitrospira was shown to be the dominant nitrite oxidizing bacteria (NOB) in all the pilot MBBR reactors at all temperatures. The performance of the post carbon removal nitrifying MBBR systems were shown to be enhanced at 1 °C by an increase in the viable embedded biomass as well as thicker biofilm. This effectively increases the number of viable cell present during low temperature operation, which partially compensates for the significant decrease in rate of ammonia removal per nitrifying cell. Operation at the highest loading conditions tested in this study at 1 °C were shown to reduce the ammonia removal rate compared to lower loading conditions at 1 °C. The lower performance at higher loading conditions at 1 °C demonstrated an enrichment in the stress response metagenomics pathways of the system.
authors.affiliation: Department of Civil Engineering, Faculty of Engineering, University of Ottawa, Ottawa, Canada.
authors.forename: Bradley
authors.initials: B
authors.lastname: Young
authors.affiliation: Department of Civil Engineering, Faculty of Engineering, University of Ottawa, Ottawa, Canada. Electronic address: robert.delatolla@uottawa.ca.
authors.forename: Robert
authors.initials: R
authors.lastname: Delatolla
authors.affiliation: Department of Civil Engineering, Faculty of Engineering, University of Ottawa, Ottawa, Canada.
authors.forename: Kevin
authors.initials: K
authors.lastname: Kennedy
authors.affiliation: Veolia Water Technologies, Montreal, Quebec, Canada.
authors.forename: Edith
authors.initials: E
authors.lastname: Laflamme
authors.affiliation: Ottawa Institute of Systems Biology, Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Canada.
authors.forename: Alain
authors.initials: A
authors.lastname: Stintzi
mesh_terms: D000641:Ammonia / Q000378:metabolism; D018441:Biofilms; D019149:Bioreactors / Q000382:microbiology; D064307:Microbiota; D058465:Nitrification; D009573:Nitrites / Q000378:metabolism; D013696:Temperature
chemical_list: D009573:Nitrites; D000641:Ammonia
keywords: Biofilm; Low temperature; MBBR; Microbiome analysis; Next generation sequencing; Nitrification