title: Insights on the structural dynamics of Leishmania braziliensis Hsp90 molecular chaperone by small angle X-ray scattering.
abstract: Heat shock protein of 90kDa (Hsp90) is an essential molecular chaperone involved in a plethora of cellular activities which modulate protein homeostasis. During the Hsp90 mechanochemical cycle, it undergoes large conformational changes, oscillating between open and closed states. Although structural and conformational equilibria of prokaryotic and some eukaryotic Hsp90s are known, some protozoa Hsp90 structures and dynamics are poorly understood. In this study, we report the solution structure and conformational dynamics of Leishmania braziliensis Hsp90 (LbHsp90) investigated by small angle X-ray scattering (SAXS). The results indicate that LbHsp90 coexists in open and closed conformations in solution and that the linkers between domains are not randomly distributed. These findings noted interesting features of the LbHsp90 system, opening doors for further conformational studies of other protozoa chaperones.
authors.affiliation: Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil.
authors.forename: Thiago V
authors.initials: TV
authors.lastname: Seraphim
authors.affiliation: Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil.
authors.forename: Kelly P
authors.initials: KP
authors.lastname: Silva
authors.affiliation: Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil.
authors.forename: Paulo R
authors.initials: PR
authors.lastname: Dores-Silva
authors.affiliation: Instituto de Física, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Leandro R S
authors.initials: LR
authors.lastname: Barbosa
authors.affiliation: Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil. Electronic address: borgesjc@iqsc.usp.br.
authors.forename: Júlio C
authors.initials: JC
authors.lastname: Borges
mesh_terms: D018841:HSP90 Heat-Shock Proteins / Q000737:chemistry* / Q000378:metabolism; D007892:Leishmania braziliensis; D008958:Models, Molecular; D000072417:Protein Domains; D015800:Protozoan Proteins / Q000737:chemistry* / Q000378:metabolism; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction
chemical_list: D018841:HSP90 Heat-Shock Proteins; D015800:Protozoan Proteins
keywords: Hsp90; Leishmania; Molecular chaperones; Multi-domain flexible proteins; SAXS