title: A benzothiazole-based fluorescent probe for distinguishing and bioimaging of Hg2+and Cu2.
abstract: A new benzothiazole-based fluorescent probe 2-(benzo[d]thiazol-2-yl)-4-(1,3- dithian-2-yl)phenol (BT) with two different reaction sites, a thioacetal group (site 1 for Hg2+), and O and N atoms of the benzothiazole dye (site 2 for Cu2+), was designed and synthesized. The probe BT showed ratiometric fluorescent response to Hg2+and fluorescence quenching behavior to Cu2+, which induces naked-eye fluorescent color changes from green to blue and colorless, respectively. Moreover, it displayed highly sensitivity and selectivity toward Hg2+and Cu2+without interference from other metal ions. The sensing mechanisms were also confirmed by1H NMR titration, mass spectrum and Job's plot analyses. Finally, probe BT was successfully used for fluorescent imaging of Hg2+and Cu2+in living cells, demonstrating its potential applications in biological science.
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Biao
authors.initials: B
authors.lastname: Gu
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Huang
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Su
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Duan
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China. Electronic address: haitao-li@hunnu.edu.cn.
authors.forename: Haitao
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Shouzhuo
authors.initials: S
authors.lastname: Yao
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry*; D003300:Copper / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007477:Ions / Q000032:analysis; D008628:Mercury / Q000032:analysis*; D013050:Spectrometry, Fluorescence
chemical_list: D052160:Benzothiazoles; D005456:Fluorescent Dyes; D007477:Ions; D003300:Copper; D008628:Mercury
keywords: Fluorescent imaging; Fluorescent probe; Hg(2+) and Cu(2+); Selectivity