title: Unenhanced areas revealed by contrast-enhanced abdominal ultrasonography with Sonazoid™ potentially correspond to colorectal cancer.
abstract: The present study investigated the potential utility of contrast-enhanced abdominal ultrasonography (CEUS), using Sonazoid™, in colorectal cancer (CRC). Three patients were subjected to CEUS with Sonazoid™. Surgical specimens were immunostained for CD31. Numbers of blood vessels positive for CD31 were analyzed in each of five fields at ×400 magnification and averaged to determine blood vessel density. Blood vessel density was compared between non-tumorous and tumorous areas. Prior to the administration of Sonazoid™, CRC was illustrated as irregular-shaped wall thickening. One minute after the administration of Sonazoid™, the majority of the thickened wall was enhanced, while some parts of the thickened wall remained unenhanced. Blood vessel densities of non-tumorous and tumorous areas in patient two were 25.2±2.5 and 5.2±1.1 (P<0.0001). Blood vessel densities of non-tumorous and tumorous areas in patient three were 19.0±3.1 and 2.2±0.8 (P<0.0001). Tumorous areas of CRC were not enhanced 1 min after the administration of Sonazoid™. Blood vessel density was lower in tumorous areas compared with non-tumorous areas, as evidenced by immunohistochemistry for CD31. These findings suggest that CEUS may be useful for the determination of the extent of CRC.
authors.affiliation: Department of Gastroenterology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido City, Chiba, 284-0003, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Tomizawa
authors.affiliation: Department of Clinical Laboratory, National Hospital Organization, Shimoshizu Hospital, Yotsukaido City, Chiba, 284-0003, Japan.
authors.forename: Mizuki
authors.initials: M
authors.lastname: Togashi
authors.affiliation: Department of Radiology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido City, Chiba, 284-0003, Japan.
authors.forename: Fuminobu
authors.initials: F
authors.lastname: Shinozaki
authors.affiliation: Department of Surgery, National Hospital Organization, Shimoshizu Hospital, Yotsukaido City, Chiba, 284-0003, Japan.
authors.forename: Rumiko
authors.initials: R
authors.lastname: Hasegawa
authors.affiliation: Department of Surgery, National Hospital Organization, Shimoshizu Hospital, Yotsukaido City, Chiba, 284-0003, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Shirai
authors.affiliation: Department of Clinical Laboratory, National Hospital Organization, Shimoshizu Hospital, Yotsukaido City, Chiba, 284-0003, Japan.
authors.forename: Midori
authors.initials: M
authors.lastname: Noritake
authors.affiliation: Department of Clinical Laboratory, National Hospital Organization, Shimoshizu Hospital, Yotsukaido City, Chiba, 284-0003, Japan.
authors.forename: Yukie
authors.initials: Y
authors.lastname: Matsuoka
authors.affiliation: Department of Clinical Laboratory, National Hospital Organization, Shimoshizu Hospital, Yotsukaido City, Chiba, 284-0003, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kainuma
authors.affiliation: Department of Clinical Laboratory, National Hospital Organization, Shimoshizu Hospital, Yotsukaido City, Chiba, 284-0003, Japan.
authors.forename: Yasuji
authors.initials: Y
authors.lastname: Iwasaki
authors.affiliation: Department of Molecular Pathology, Chiba University Graduate School of Medicine, Chiba City, Chiba 260-8670, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Fugo
authors.affiliation: Department of Neurology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Yotsukaido City, Chiba 284-0003, Japan.
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Motoyoshi
authors.affiliation: Department of Rheumatology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Yotsukaido City, Chiba 284-0003, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Sugiyama
authors.affiliation: Department of Pediatrics, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Yotsukaido City, Chiba 284-0003, Japan.
authors.forename: Shigenori
authors.initials: S
authors.lastname: Yamamoto
authors.affiliation: Department of Molecular Pathology, Chiba University Graduate School of Medicine, Chiba City, Chiba 260-8670, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kishimoto
authors.affiliation: Department of Neurosurgery, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Yotsukaido City, Chiba 284-0003, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Ishige
keywords: Sonazoid™; blood vessel; cluster of differentiation 31; colorectal cancer; density; perfluorocarbon microbubbles