title: The effect of dissolved organic matter on soybean peroxidase-mediated removal of triclosan in water.
abstract: Dissolved organic matter (DOM) is ubiquitous in water and involved in numerous important chemical processes in aqueous systems, enabling it a unique challenge for a variety of water treatment processes. Soybean peroxidase (SBP)-based enzymatic process, as a promising treatment technique, has been successfully applied to remove pollutants in wastewaters such as coal-tar and refinery wastewater. In this study, the effect of DOM on the removal of polychlorinated aromatic antimicrobials triclosan (TCS) by SBP was investigated. Our results suggested that DOM significantly suppressed the catalytic performance of SBP to TCS, presumably resulting from the competition of the phenolic moiety in DOM structure as the active substrate of SBP via the analysis of excitation emission matrix (EEM) spectra of DOM. Although the product species of TCS in SBP-mediated system with DOM has no change compared with the system without DOM, the yields of self-coupling products relative to total transformed TCS were remarkably reduced in the presence of DOM, suggesting that DOM participated in the oxidative coupling reactions. Cross-coupling between TCS and DOM was also verified using guaiacol as a model DOM constituent. Moreover, the products including self-coupling products and co-polymers in SBP-mediated TCS reaction system with DOM were innocuous through growth inhibition assay of S. obliquus.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li
authors.affiliation: Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection of the People's Republic of China, Nanjing, 210042, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, China.
authors.forename: Jianbiao
authors.initials: J
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, China.
authors.forename: Xinan
authors.initials: X
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, China. Electronic address: ecsxg@nju.edu.cn.
authors.forename: Shixiang
authors.initials: S
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Mao
mesh_terms: D019149:Bioreactors; D002384:Catalysis; D000460:Chlorophyta / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D003033:Coal Tar; D004396:Coloring Agents / Q000032:analysis; D006139:Guaiacol / Q000737:chemistry; D007700:Kinetics; D013058:Mass Spectrometry; D010544:Peroxidases / Q000737:chemistry*; D019800:Phenol / Q000737:chemistry; D013025:Glycine max / Q000201:enzymology*; D014260:Triclosan / Q000032:analysis*; D062065:Wastewater / Q000032:analysis; D014867:Water / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*; D018508:Water Purification
chemical_list: D003033:Coal Tar; D004396:Coloring Agents; D006139:Guaiacol; D010544:Peroxidases; D019800:Phenol; D014260:Triclosan; D062065:Wastewater; D014867:Water; D014874:Water Pollutants, Chemical; C120494:anionic peroxidase
keywords: Dissolved organic matter; Soybean peroxidase; Triclosan; Wastewater treatment