title: Combined utilization of nutrients and sugar derived from wheat bran for d-Lactate fermentation by Sporolactobacillus inulinus YBS1-5.
abstract: To decrease d-Lactate production cost, wheat bran, a low-cost waste of milling industry, was selected as the sole feedstock. First, the nutrients were recovered from wheat bran by acid protease hydrolysis. Then, cellulosic hydrolysates were prepared from protease-treated samples after acid pretreatment and enzymatic saccharification. The combined use of nutrients and hydrolysates as nitrogen and carbon sources for fermentation by S. inulinus YB1-5 resulted in d-Lactate levels of 99.5g/L, with an average production efficiency of 1.94g/L/h and a yield of 0.89g/g glucose. Moreover, fed-batch simultaneous saccharification and fermentation process at 40°C, 20% (w/v) solid loading and 20FPU/g solid cellulase concentration was obtained. d-Lactate concentrations, yield, productivity, and optical purity were 87.3g/L, 0.65g/g glucose, 0.81g/L/h and 99.1%, respectively. This study provided a feasible procedure that can help produce cellulosic d-Lactate using agricultural waste without external nutrient supplementation.
authors.affiliation: School of Life Sciences, Nanjing University, 163 Xianlin Road, Nanjing 210023, Jiangsu, China.
authors.forename: Jiahuang
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 30 Puzhunan Road, Nanjing 211816, Jiangsu, China.
authors.forename: Junfei
authors.initials: J
authors.lastname: Sun
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 30 Puzhunan Road, Nanjing 211816, Jiangsu, China; Jiangsu National Synergetic Innovation Center for Advanced Materials, 30 Puzhunan Road, Nanjing 211816, Jiangsu, China. Electronic address: wbwb11@163.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.affiliation: Jiangsu National Synergetic Innovation Center for Advanced Materials, 30 Puzhunan Road, Nanjing 211816, Jiangsu, China; School of Pharmaceutical Sciences, Nanjing Tech University, 30 Puzhunan Road, Nanjing 211816, Jiangsu, China.
authors.forename: Bingfang
authors.initials: B
authors.lastname: He
mesh_terms: D056449:Bacillales / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry*; D002480:Cellulase / Q000378:metabolism; D003365:Costs and Cost Analysis; D004043:Dietary Fiber / Q000032:analysis*; D005285:Fermentation; D006868:Hydrolysis; D019344:Lactic Acid / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism; D013997:Time Factors
chemical_list: D002241:Carbohydrates; D004043:Dietary Fiber; D019344:Lactic Acid; D002480:Cellulase; D010447:Peptide Hydrolases
keywords: Enzymatic saccharification; Nutrient recovery; Sporolactobacillus inulinus; Wheat bran; d-Lactate