title: Expression and localization of fibroblast growth factor (FGF)23 and Klotho in the spleen: its physiological and functional implications.
abstract: The FGF23-Klotho signaling axis is known to exert anti-aging effects via calcium-phosphorus metabolism. In mice deficient in FGF23-Klotho signaling, however, the number of splenocytes is reduced. FGF23 is expressed in both bone and spleen, with regulation of its production differing in these organs. As FGF23-Klotho signaling may play an immunological role in the spleen, splenocytes in male C57BL/6J mice were assayed for expression of Klotho or FGF23 by flow cytometry and immunohistochemistry. Cells that expressed Klotho included CD45R/B220+CD21/CD35+CD1d+CD43-marginal zone B cells. These cells also expressed FGF receptor 1, indicating that Klotho-positive B cells could respond to FGF23. Plasmacytoid dendritic cells (pDCs) with CD11c+CD45R/B220+CD11b-CD8α-were found to produce FGF23. Klotho-positive cells and FGF23-producing cells were present in close proximity to each other, suggesting that FGF23 produced by pDCs may act within a limited area. These findings indicate that FGF23-Klotho signaling could play a biological or immunological role in the spleen.
authors.affiliation: a Department of Nephrology , Wakayama Medical University , Wakayama , Japan.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Nakashima
authors.affiliation: a Department of Nephrology , Wakayama Medical University , Wakayama , Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Mima
authors.affiliation: a Department of Nephrology , Wakayama Medical University , Wakayama , Japan.
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Yashiro
authors.affiliation: a Department of Nephrology , Wakayama Medical University , Wakayama , Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Sonou
authors.affiliation: a Department of Nephrology , Wakayama Medical University , Wakayama , Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Ohya
authors.affiliation: a Department of Nephrology , Wakayama Medical University , Wakayama , Japan.
authors.forename: Asuka
authors.initials: A
authors.lastname: Masumoto
authors.affiliation: a Department of Nephrology , Wakayama Medical University , Wakayama , Japan.
authors.forename: Shintaro
authors.initials: S
authors.lastname: Yamanaka
authors.affiliation: a Department of Nephrology , Wakayama Medical University , Wakayama , Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Koreeda
authors.affiliation: a Department of Nephrology , Wakayama Medical University , Wakayama , Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Tatsuta
authors.affiliation: a Department of Nephrology , Wakayama Medical University , Wakayama , Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Hanba
authors.affiliation: a Department of Nephrology , Wakayama Medical University , Wakayama , Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Moribata
authors.affiliation: a Department of Nephrology , Wakayama Medical University , Wakayama , Japan.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Negi
authors.affiliation: a Department of Nephrology , Wakayama Medical University , Wakayama , Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Shigematsu
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D003713:Dendritic Cells / Q000378:metabolism; D000089703:Fibroblast Growth Factor-23; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000378:metabolism*; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism*; D000090265:Klotho Proteins; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000378:metabolism*
chemical_list: C000717428:Fgf23 protein, mouse; D017468:Receptors, Fibroblast Growth Factor; D005346:Fibroblast Growth Factors; D000089703:Fibroblast Growth Factor-23; D005966:Glucuronidase; D000090265:Klotho Proteins
keywords: B cells; FGF23; Klotho; dendritic cells; splenocytes