title: Self-Assembly of Hierarchical DNA Nanotube Architectures with Well-Defined Geometries.
abstract: An essential motif for the assembly of biological materials such as actin at the scale of hundreds of nanometers and beyond is a network of one-dimensional fibers with well-defined geometry. Here, we demonstrate the programmed organization of DNA filaments into micron-scale architectures where component filaments are oriented at preprogrammed angles. We assemble L-, T-, and Y-shaped DNA origami junctions that nucleate two or three micron length DNA nanotubes at high yields. The angles between the nanotubes mirror the angles between the templates on the junctions, demonstrating that nanoscale structures can control precisely how micron-scale architectures form. The ability to precisely program filament orientation could allow the assembly of complex filament architectures in two and three dimensions, including circuit structures, bundles, and extended materials.
authors.affiliation: Chemical and Biomolecular Engineering and ‡Computer Science, Johns Hopkins University , Baltimore, Maryland 21218, United States.
authors.forename: Tyler D
authors.initials: TD
authors.lastname: Jorgenson
authors.affiliation: Chemical and Biomolecular Engineering and ‡Computer Science, Johns Hopkins University , Baltimore, Maryland 21218, United States.
authors.forename: Abdul M
authors.initials: AM
authors.lastname: Mohammed
authors.affiliation: Chemical and Biomolecular Engineering and ‡Computer Science, Johns Hopkins University , Baltimore, Maryland 21218, United States.
authors.forename: Deepak K
authors.initials: DK
authors.lastname: Agrawal
authors.affiliation: Chemical and Biomolecular Engineering and ‡Computer Science, Johns Hopkins University , Baltimore, Maryland 21218, United States.
authors.forename: Rebecca
authors.identifier: 0000-0003-4555-3162
authors.initials: R
authors.lastname: Schulman
mesh_terms: D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry*; D043942:Nanotubes / Q000737:chemistry*; D010316:Particle Size
chemical_list: D004247:DNA
keywords: DNA nanotechnology; DNA nanotubes; DNA origami; nanotube junctions; programmable nanostructures; self-assembly