title: Long-Distance Travellers: Phylogeography of a Generalist Parasite, Pholeter gastrophilus, from Cetaceans.
abstract: We studied the phylogeography and historical demography of the most generalist digenean from cetaceans, Pholeter gastrophilus, exploring the effects of isolation by distance, ecological barriers and hosts' dispersal ability on the population structure of this parasite. The ITS2 rDNA, and the mitochondrial COI and ND1 from 68 individual parasites were analysed. Worms were collected from seven oceanic and coastal cetacean species from the south western Atlantic (SWA), central eastern Atlantic, north eastern Atlantic (NEA), and Mediterranean Sea. Pholeter gastrophilus was considered a single lineage because reciprocal monophyly was not detected in the ML cladogram of all individuals, and sequence variability was <1% for mtDNA and 0% for ITS2. These results rule out a recent suggestion that P. gastrophilus would actually be a cryptic-species complex. The genetic cohesion of P. gastrophilus could rely on the extensive exploitation of wide-ranging and highly mobile cetaceans, with a putative secondary role, if any, of intermediate hosts. Unique haplotypes were detected in SWA and NEA, and an AMOVA revealed significant population structure associated to the genetic variation in these regions. The Equator possibly acts as a significant geographical barrier for cetacean movements, possibly limiting gene flow between northern and southern populations of P. gastrophilus. A partial Mantel tests revealed that the significant isolation of NEA populations resulted from geographic clustering. Apparently, the limited mobility of cetaceans used by P. gastrophilus as definitive hosts in this region, coupled with oceanographic barriers and a patchy distribution of potential intermediate hosts could contribute to significant ecological isolation of P. gastrophilus in NEA. Rather unexpectedly, no genetic differentiation was found in the Mediterranean samples of this parasite. Historical demographic analyses suggested a recent population expansion of P. gastrophilus in the Atlantic Ocean, perhaps linked to initial association and subsequent spreading in cetaceans.
authors.affiliation: Cavanilles Institute of Biodiversity and Evolutionary Biology, Science Park, University of Valencia, Paterna, Valencia, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: Fraija-Fernández
authors.affiliation: Cavanilles Institute of Biodiversity and Evolutionary Biology, Science Park, University of Valencia, Paterna, Valencia, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Fernández
authors.affiliation: Institute for Terrestrial and Aquatic Wildlife Research, University of Veterinary Medicine Hannover, Werftstrasse, Büsum, Germany.
authors.forename: Kristina
authors.initials: K
authors.lastname: Lehnert
authors.affiliation: Cavanilles Institute of Biodiversity and Evolutionary Biology, Science Park, University of Valencia, Paterna, Valencia, Spain.
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: Raga
authors.affiliation: Institute for Terrestrial and Aquatic Wildlife Research, University of Veterinary Medicine Hannover, Werftstrasse, Büsum, Germany.
authors.forename: Ursula
authors.initials: U
authors.lastname: Siebert
authors.affiliation: Cavanilles Institute of Biodiversity and Evolutionary Biology, Science Park, University of Valencia, Paterna, Valencia, Spain.
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Aznar
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001267:Atlantic Ocean; D002591:Cetacea / Q000469:parasitology*; D018509:DNA, Helminth / Q000737:chemistry; D003710:Demography; D049810:Genetic Speciation; D014644:Genetic Variation; D016013:Likelihood Functions; D008522:Mediterranean Sea; D058974:Phylogeography; D011157:Population Dynamics; D060047:Reproductive Isolation; D017422:Sequence Analysis, DNA; D014322:Troglotrematidae / Q000145:classification / Q000235:genetics* / Q000502:physiology
chemical_list: D018509:DNA, Helminth