title: [Effect of methyleugenol on expression of MUC5AC in nasal mucosa of rats with allergic rhinitis].
abstract: Objective:To investigate the effect of methyleugenol on expression of MUC5AC in nasal mucosa of rats with allergic rhinitis (AR).Methods:Seventy-two Wistar rats were randomly divided into 6 groups:normal control group, AR group, loratadine group, low-dose methyleugenol group, middle-dose methyleugenol group and high-dose methyleugenol group with 12 rats in each group. AR was induced by intraperitoneal injection of ovalbumin in latter 5 groups. 10 mg loratadine q.d was given to rats in loratadine group by gavage; and 10 mg/kg, 20 mg/kg and 40 mg/kg methyleugenol were given by gavege q.d to rats in low-, middle-and high-dose methyleugenol groups, respectively. Nasal mucosa samples were obtained from rats at 1, 2, 4 and 6 weeks after drug intervention. The expression of MUC5AC protein and mRNA in nasal mucosa was detected by immunohistochemistry and real-time fluorescence quota PCR (RT-PCR), respectively.Results:Compared with AR, the percentage of cells staining positively for MUC5AC protein and the relative quantity of MUC5AC mRNA in middle-and high-dose methyleugenol groups were significantly decreased after 2 and 4 weeks of drug intervention (P<0.05), but no such decrease was observed in low-dose methyleugenol group at all time points (P>0.05). The percentage of cells with positive expression of MUC5AC protein and mRNA in loratadine group were significantly decreased after 1 week of administration (P<0.05). The percentage of cells with positive MUC5AC protein in middle-dose methyleugenol group was higher than that in loratadine group (P<0.05) after 6 week of drug intervention, but the difference was not seen in high-dose group (P>0.05). There was no significant difference in relative quantities of MUC5AC mRNA after 4 weeks of administration between high-and middle-dose methyeugenol groups and loratadine group (P>0.05).Conclusion:Methyleugenol can attenuate AR through inhibiting the expression of MUC5AC mRNA and protein in nasal mucosa of AR rats.
authors.affiliation: Department of Child Care, the First Hospital of Lanzhou University, Lanzhou 730000, China.
authors.forename: Nannan
authors.initials: N
authors.lastname: Meng
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, the First Hospital of Lanzhou University, Lanzhou 730000, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, the First Hospital of Lanzhou University, Lanzhou 730000, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Gui
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, the First Hospital of Lanzhou University, Lanzhou 730000, China.
authors.forename: Kehu
authors.initials: K
authors.lastname: Xi
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, the First Hospital of Lanzhou University, Lanzhou 730000, China.
authors.forename: Youhu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, the First Hospital of Lanzhou University, Lanzhou 730000, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Child Care, the First Hospital of Lanzhou University, Lanzhou 730000, China. CH701@163.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, the First Hospital of Lanzhou University, Lanzhou 730000, China. 790736924@qq.com.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects*; D005054:Eugenol / Q000031:analogs & derivatives* / Q000494:pharmacology; D017336:Loratadine; D055271:Mucin 5AC / Q000187:drug effects* / Q000502:physiology; D009297:Nasal Mucosa / Q000737:chemistry; D010047:Ovalbumin; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D065631:Rhinitis, Allergic / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology
chemical_list: C524680:Muc5ac protein, rat; D055271:Mucin 5AC; C005223:methyleugenol; D005054:Eugenol; D017336:Loratadine; D010047:Ovalbumin