title: Degenerative effects of cobalt-chloride treatment on neurons and microglia in a porcine retina organ culture model.
abstract: In order to understand the pathological processes of retinal diseases, experimental models are necessary. Cobalt, as part of the vitamin B12 complex, is important for neuronal integrity. However, it is known that high quantities of cobalt induce cytotoxic mechanisms via hypoxia mimicry. Therefore, we tested the degenerative effect of cobalt chloride (CoCl2) on neurons and microglia in a porcine retina organ culture model. Organotypic cultures of porcine retinas were cultured and treated with different concentrations of CoCl2(0, 100, 300 and 500 μM) for 48 h. After four and eight days, CoCl2induced a strong degeneration of the porcine retina, starting at 300 μM. A loss of retinal ganglion cells (RGCs, Brn-3a), amacrine cells (calretinin) and bipolar cells (PKCα) was observed. Additionally, a high expression of hypoxia induced factor-1a (HIF-1a) and heat shock protein 70 (HSP70) was noted at both points in time. Also, the Caspase 3 protein was activated and P21 expression was induced. However, only at day four, the Bax/Bcl-2 ratio was increased. The effect of CoCl2was not restricted to neurons. CoCl2concentrations reduced the microglia amount (Iba1) and activity (Iba1 + Fcγ-Receptor) at both points in time. These damaging effects on microglia were surprising, since CoCl2causes hypoxia and a pro-inflammatory environment. However, high concentrations of CoCl2also seem to be toxic to these cells. Similar degenerative mechanisms as in comparison to retinal ischemia animal models were observed. In summary, an effective and reproducible hypoxia-mimicking organotypic model for retinal degeneration was established, which is easy to handle and ready for drug studies.
authors.affiliation: Experimental Eye Research, Eye Hospital, Ruhr-University Bochum, In der Schornau 23-25, 44892 Bochum, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Kuehn
authors.affiliation: University Eye Hospital Tübingen, Centre for Ophthalmology, Elfriede-Aulhorn-Str. 7, 72076, Tübingen, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Hurst
authors.affiliation: Experimental Eye Research, Eye Hospital, Ruhr-University Bochum, In der Schornau 23-25, 44892 Bochum, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Rensinghoff
authors.affiliation: Experimental Eye Research, Eye Hospital, Ruhr-University Bochum, In der Schornau 23-25, 44892 Bochum, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Tsai
authors.affiliation: Experimental Eye Research, Eye Hospital, Ruhr-University Bochum, In der Schornau 23-25, 44892 Bochum, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Grauthoff
authors.affiliation: Experimental Eye Research, Eye Hospital, Ruhr-University Bochum, In der Schornau 23-25, 44892 Bochum, Germany.
authors.forename: Y
authors.initials: Y
authors.lastname: Satgunarajah
authors.affiliation: Experimental Eye Research, Eye Hospital, Ruhr-University Bochum, In der Schornau 23-25, 44892 Bochum, Germany.
authors.forename: H B
authors.initials: HB
authors.lastname: Dick
authors.affiliation: University Eye Hospital Tübingen, Centre for Ophthalmology, Elfriede-Aulhorn-Str. 7, 72076, Tübingen, Germany. Electronic address: sven.schnichels@med.uni-tuebingen.de.
authors.forename: S
authors.initials: S
authors.lastname: Schnichels
authors.affiliation: Experimental Eye Research, Eye Hospital, Ruhr-University Bochum, In der Schornau 23-25, 44892 Bochum, Germany. Electronic address: stephanie.joachim@rub.de.
authors.forename: S C
authors.initials: SC
authors.lastname: Joachim
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000009:adverse effects; D017209:Apoptosis; D015153:Blotting, Western; D002470:Cell Survival; D003035:Cobalt / Q000009:adverse effects*; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000096:biosynthesis / Q000235:genetics*; D007150:Immunohistochemistry; D017628:Microglia / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D009924:Organ Culture Techniques; D012313:RNA / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012162:Retinal Degeneration / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D012165:Retinal Ganglion Cells / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D055351:Retinal Neurons / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D013552:Swine
chemical_list: D016587:Antimutagenic Agents; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D017382:Reactive Oxygen Species; D003035:Cobalt; D012313:RNA; C018021:cobaltous chloride
keywords: Amacrine cells; Bipolar cells; Cobalt-chloride; Degeneration; Microglia; Pig; Retina; Retina organ culture; Retinal ganglion cells