title: Post-translational selective intracellular silencing of acetylated proteins with de novo selected intrabodies.
abstract: The ability to selectively interfere with post-translationally modified proteins would have many biological and therapeutic applications. However, post-translational modifications cannot be selectively targeted by nucleic-acid-based interference approaches. Here we describe post-translational intracellular silencing antibody technology (PISA), a method for selecting intrabodies against post-translationally modified proteins. We demonstrate our method by generating intrabodies against native acetylated proteins and showing functional interference in living cells.
authors.affiliation: Bio@SNS Laboratory, Scuola Normale Superiore, Pisa, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Chirichella
authors.affiliation: Bio@SNS Laboratory, Scuola Normale Superiore, Pisa, Italy.
authors.forename: Simonetta
authors.initials: S
authors.lastname: Lisi
authors.affiliation: Bio@SNS Laboratory, Scuola Normale Superiore, Pisa, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Fantini
authors.affiliation: Bio@SNS Laboratory, Scuola Normale Superiore, Pisa, Italy.
authors.forename: Martina
authors.initials: M
authors.lastname: Goracci
authors.affiliation: Bio@SNS Laboratory, Scuola Normale Superiore, Pisa, Italy.
authors.forename: Mariantonietta
authors.initials: M
authors.lastname: Calvello
authors.affiliation: Genomic Facility, European Brain Research Institute (EBRI), Roma, Italy.
authors.forename: Rossella
authors.initials: R
authors.lastname: Brandi
authors.affiliation: Genomic Facility, European Brain Research Institute (EBRI), Roma, Italy.
authors.forename: Ivan
authors.initials: I
authors.lastname: Arisi
authors.affiliation: Genomic Facility, European Brain Research Institute (EBRI), Roma, Italy.
authors.forename: Mara
authors.initials: M
authors.lastname: D'Onofrio
authors.affiliation: Bio@SNS Laboratory, Scuola Normale Superiore, Pisa, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Di Primio
authors.affiliation: Bio@SNS Laboratory, Scuola Normale Superiore, Pisa, Italy.
authors.forename: Antonino
authors.initials: A
authors.lastname: Cattaneo
mesh_terms: D000107:Acetylation; D000906:Antibodies / Q000276:immunology*; D019427:HIV Integrase / Q000276:immunology* / Q000378:metabolism*; D006657:Histones / Q000276:immunology* / Q000378:metabolism*; D006801:Humans; D011499:Protein Processing, Post-Translational / Q000276:immunology*
chemical_list: D000906:Antibodies; D006657:Histones; D019427:HIV Integrase; C514801:p31 integrase protein, Human immunodeficiency virus 1