title: Neither quantification by qPCR nor quantitative Elisa can be used to discriminate Angus cattle for resistance/susceptibility to Babesia bovis.
abstract: With the aim of finding quantitative phenotypic traits that can be used to discriminate the levels of resistance/susceptibility to Babesia bovis, we estimated the repeatability and correlation between the level of infection, determined by the number of copies of a fragment of the gene that encodes cytochrome B (NC mt-cyB) of B. bovis, and the levels of the anti-B. bovis antibodies, in blood samples collected from 51 Angus cattle on two different occasions. Samples with the anticoagulant EDTA were used for DNA extraction and without anticoagulant for separation of the blood serum. The quantification of the NC mt-cyB of B. bovis was carried out by the quantitative PCR technique (qPCR), while the anti-B. bovis IgG antibody titers (S/P) were quantified by the ELISA method. The NC and S/P data were log10-transformed to improve the approximation to the normal distribution and were analyzed using mixed models. The correlations between NC mt-cyB and S/P were estimated, as well as the repeatability values for each trait. The results obtained showed the high sensitivity of the techniques, with 100% of the animals being positive for B. bovis, detected by both the serological and molecular tests. The correlations estimated between NC and S/P were low, 0.10 and 0.12, in the first and second collection, respectively. The repeatability estimated for NC was 0.06, whereas for the S/P it was 0.42. The low correlations between S/P and NC in the two collections demonstrated that the variation in the NC value is independent of the level of antibodies. This results indicated that animals with a higher levels of antibodies against B. bovis in the first collection continued to have a higher levels in the second one. However, the very low values for the repeatability value of NC, and for the correlations between S/P and NC, demonstrates that neither NC or S/P could be used to discriminate animals for resistance/susceptibility to B. bovis.
authors.affiliation: Universidade Estadual Júlio de Mesquita Filho, Jaboticabal, SP, Brazil. Electronic address: gigliotirodrigo@gmail.com.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Giglioti
authors.affiliation: Universidade Estadual Júlio de Mesquita Filho, Jaboticabal, SP, Brazil.
authors.forename: Henrique Nunes
authors.initials: HN
authors.lastname: Oliveira
authors.affiliation: Embrapa Suínos e Aves, Concórdia, SC, Brazil.
authors.forename: Adriana Mércia Guaratini
authors.initials: AMG
authors.lastname: Ibelli
authors.affiliation: Universidade Estadual Júlio de Mesquita Filho, Jaboticabal, SP, Brazil.
authors.forename: Talita Barban
authors.initials: TB
authors.lastname: Bilhassi
authors.affiliation: Universidade Federal de, São Carlos, SP, Brazil.
authors.forename: Thalita Athiê
authors.initials: TA
authors.lastname: Néo
authors.affiliation: Universidade Estadual Júlio de Mesquita Filho, Jaboticabal, SP, Brazil.
authors.forename: Clarissa Helena
authors.initials: CH
authors.lastname: Santana
authors.affiliation: Embrapa Pecuária Sudeste, São Carlos, SP, Brazil.
authors.forename: Márcio Dias
authors.initials: MD
authors.lastname: Rabelo
authors.affiliation: Universidade Estadual Júlio de Mesquita Filho, Jaboticabal, SP, Brazil.
authors.forename: Rosângela Zacarias
authors.initials: RZ
authors.lastname: Machado
authors.affiliation: Embrapa Pecuária Sudeste, São Carlos, SP, Brazil.
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: de Souza Chagas
authors.affiliation: Embrapa Pecuária Sudeste, São Carlos, SP, Brazil.
authors.forename: Márcia Cristina
authors.initials: MC
authors.lastname: de Sena Oliveira
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D016793:Babesia bovis / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D001404:Babesiosis / Q000276:immunology* / Q000469:parasitology; D002417:Cattle / Q000469:parasitology; D002418:Cattle Diseases / Q000276:immunology* / Q000469:parasitology; D045303:Cytochromes b / Q000235:genetics; D016054:DNA, Protozoan / Q000097:blood; D060467:Disease Resistance; D004198:Disease Susceptibility; D004797:Enzyme-Linked Immunosorbent Assay; D025202:Molecular Diagnostic Techniques / Q000379:methods; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
chemical_list: D000913:Antibodies, Protozoan; D016054:DNA, Protozoan; D045303:Cytochromes b
keywords: Babesia bovis; Beef cattle; Correlations; Repeatability; Resistance