title: Comparative analysis of NK cell receptor repertoire in adults and very elderly subjects with cytomegalovirus infection.
abstract: Human cytomegalovirus (HCMV) infection in children and young adults has been associated with changes in the innate immune system. We herein analyzed the possible effect of very long term HCMV infection on the expression of several NK cell receptors. Ninety HCMV-seropositive individuals were included and classified as young adults (n=30), elderly (n=30) and very elderly subjects (n=30). A peripheral blood sample was obtained and the expression of NK cell receptors (NKG2A, NKG2C, ILT2, CD161, KIR2DL1, KIR3DL1, and KIR3DL2) by NK and other lymphocyte subsets was assessed by flow cytometry. In addition, the frequency of the sixteen KIR genes was analyzed by polymerase chain reaction. We found a significant increase in the number of NKG2C+ NK and T cells in elderly individuals compared to young adults accompanied by an opposite trend in the number of NKG2A+ lymphocytes, and ILT2+ cells were also increased in elderly individuals. A significant increase in the levels of CD3-CD56+NKG2C+CD57+ cells was also detected in the elderly groups. Finally, KIR gene analysis revealed that the KIR genotype 2 was significantly less frequent in the elderly individuals. Our results support that long-term infection by HCMV exerts a significant progressive effect on the innate immune system.
authors.affiliation: Department of Immunology, School of Medicine, UASLP, San Luis Potosí, SLP, Mexico.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Juárez-Vega
authors.affiliation: Department of Microbiology, School of Medicine, UASLP, San Luis Potosí, SLP, Mexico.
authors.forename: Velia
authors.initials: V
authors.lastname: Rangel-Ramírez
authors.affiliation: Department of Immunology, School of Medicine, UASLP, San Luis Potosí, SLP, Mexico; Research Center for Health Sciences and Biomedicine, UASLP, San Luis Potosí, SLP, Mexico.
authors.forename: Adriana
authors.initials: A
authors.lastname: Monsiváis-Urenda
authors.affiliation: Department of Immunology, School of Medicine, UASLP, San Luis Potosí, SLP, Mexico; Research Center for Health Sciences and Biomedicine, UASLP, San Luis Potosí, SLP, Mexico.
authors.forename: Perla
authors.initials: P
authors.lastname: Niño-Moreno
authors.affiliation: Laboratory of Viral and Human Genomics, School of Medicine, UASLP, San Luis Potosí, SLP, Mexico.
authors.forename: Christian
authors.initials: C
authors.lastname: Garcia-Sepúlveda
authors.affiliation: Department of Microbiology, School of Medicine, UASLP, San Luis Potosí, SLP, Mexico.
authors.forename: Daniel E
authors.initials: DE
authors.lastname: Noyola
authors.affiliation: Department of Immunology, School of Medicine, UASLP, San Luis Potosí, SLP, Mexico; Research Center for Health Sciences and Biomedicine, UASLP, San Luis Potosí, SLP, Mexico. Electronic address: rgonzale@uaslp.mx.
authors.forename: Roberto
authors.initials: R
authors.lastname: González-Amaro
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000276:immunology* / Q000502:physiology; D003586:Cytomegalovirus Infections / Q000235:genetics / Q000276:immunology* / Q000821:virology; D005260:Female; D005434:Flow Cytometry; D005787:Gene Frequency; D005838:Genotype; D006239:Haplotypes; D054884:Host-Pathogen Interactions / Q000276:immunology; D006801:Humans; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D055607:Receptors, Natural Killer Cell / Q000145:classification / Q000235:genetics / Q000276:immunology*; D055815:Young Adult
chemical_list: D055607:Receptors, Natural Killer Cell
keywords: Cytomegalovirus; Innate immunity; NK cells