title: BDE-47 and BDE-209 inhibit proliferation of Neuro-2a cells via inducing G1-phase arrest.
abstract: Cell proliferation is closely related to cell cycle which is strictly regulated by genes and regulatory proteins. In the present study, we comparatively analyzed the toxic effects of BDE-47 and BDE-209 on cell proliferation of Neuro-2a cells, and the possible mechanism was discussed. The results indicated that BDE-47 significantly inhibited the cell proliferation and the cell cycle were arrest at G1 phase, while BDE-209 had little effects on either cell proliferation or cell cycle. qRT-PCR and Western blot assay presented that BDE-47 up-regulated the gene expressions of p53 and p21, which down-regulated the expresseion of cyclinD1 and CDK2, and inhibited retinoblastoma protein (pRb) phosphorylation. This process could effectively arrest the cell cycle at G1 phase, which finally caused the inhibition on Neuro-2a cell proliferation. However, BDE-209 was only up-regulated the gene expressions of p53, also suggested to be involved in the inhibition on Neuro-2a cell proliferation.
authors.affiliation: Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
authors.forename: Xuexi
authors.initials: X
authors.lastname: Tang
authors.affiliation: Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
authors.forename: Ningning
authors.initials: N
authors.lastname: Xu
authors.affiliation: Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
authors.forename: Zhongyuan
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
authors.forename: Kuan
authors.initials: K
authors.lastname: Fang
authors.affiliation: Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China. Electronic address: wangyou@ouc.edu.cn.
authors.forename: You
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D051357:Cyclin-Dependent Kinase 2 / Q000235:genetics / Q000378:metabolism; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics / Q000378:metabolism; D059585:G1 Phase Cell Cycle Checkpoints / Q000187:drug effects*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D055768:Halogenated Diphenyl Ethers / Q000494:pharmacology*; D051379:Mice; D016160:Retinoblastoma Protein / Q000235:genetics / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: C530105:Ccnd1 protein, mouse; D050759:Cyclin-Dependent Kinase Inhibitor p21; D055768:Halogenated Diphenyl Ethers; D016160:Retinoblastoma Protein; D016159:Tumor Suppressor Protein p53; C511295:2,2',4,4'-tetrabromodiphenyl ether; D019938:Cyclin D1; C495893:Cdk2 protein, mouse; D051357:Cyclin-Dependent Kinase 2; C010902:decabromobiphenyl ether
keywords: 2,2′ -,4,4′-tetrabromodiphenyl ether (BDE-47); Cell cycle; Cell proliferation; Decabrominateddiphenyl ether (BDE-209); G1-phase arrest; Neuro-2a cells