title: Sex-dependent telomere shortening, telomerase activity and oxidative damage in marine medaka Oryzias melastigma during aging.
abstract: Marine medaka Oryzias melastigma at 4months (young), 8months (middle-aged) and 12months old (senior) were employed to determine age-associated change of sex ratios, sex hormones, telomere length (TL), telomerase activity (TA), telomerase transcription (omTERT) and oxidative damage in the liver. Overall, O. melastigma exhibited gradual senescence, sex differences in longevity (F>M), TL (F>M) and oxidative damage (F<M) during aging. In females, the plasma E2 level was positively correlated with TL (TRF>5kb), TA and omTERT expression (p≤0.01), and negatively correlated with liver DNA oxidation (p≤0.05). The results suggest high levels of E2 in female O. melastigma may retard TL shortening by enhancing TA via TERT transcription and/or reducing oxidative DNA damage. The findings support TL shortening as a biomarker of aging and further development of accelerated TL shortening, abnormal suppression of TA and excessive oxidative DNA damage as early molecular endpoints, indicative of advanced/premature aging in marine medaka/fish.
authors.affiliation: State Key Laboratory in Marine Pollution, Department of Biology and Chemistry, City University of Hong Kong, China.
authors.forename: Bill Wp
authors.initials: BW
authors.lastname: Yip
authors.affiliation: State Key Laboratory in Marine Pollution, Department of Biology and Chemistry, City University of Hong Kong, China.
authors.forename: Helen Ol
authors.initials: HO
authors.lastname: Mok
authors.affiliation: State Key Laboratory in Marine Pollution, Department of Biology and Chemistry, City University of Hong Kong, China.
authors.forename: Drew R
authors.initials: DR
authors.lastname: Peterson
authors.affiliation: State Key Laboratory in Marine Pollution, Department of Biology and Chemistry, City University of Hong Kong, China.
authors.forename: Miles T
authors.initials: MT
authors.lastname: Wan
authors.affiliation: Department of Preventive Medicine and Public Health, School of Medicine, Kyorin University, Tokyo 181-8611, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Taniguchi
authors.affiliation: Centre of Reproduction, Development and Aging, Faculty of Health Sciences, University of Macau, Avenida da Universidade, Taipa, Macau, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ge
authors.affiliation: State Key Laboratory in Marine Pollution, Department of Biology and Chemistry, City University of Hong Kong, China. Electronic address: bhdwtau@cityu.edu.hk.
authors.forename: Doris Wt
authors.initials: DW
authors.lastname: Au
mesh_terms: D000375:Aging; D000818:Animals; D004967:Estrogens / Q000378:metabolism*; D005260:Female; D008297:Male; D009990:Oryzias / Q000502:physiology*; D018384:Oxidative Stress; D012737:Sex Factors; D019098:Telomerase / Q000378:metabolism*; D059506:Telomere Shortening; D013739:Testosterone / Q000378:metabolism*
chemical_list: D004967:Estrogens; D013739:Testosterone; D019098:Telomerase
keywords: 8-OHdG; Biomarker of aging; Longevity gender gap; Post-menopausal decline of E2; Protein carbonyl; Sex hormones; TERT; Telomerase and telomere