title: Comparative Characterization of Phosphatidic Acid Sensors and Their Localization during Frustrated Phagocytosis.
abstract: Phosphatidic acid (PA) is the simplest phospholipid naturally existing in living organisms, but it constitutes only a minor fraction of total cell lipids. PA has attracted considerable attention because it is a phospholipid precursor, a lipid second messenger, and a modulator of membrane shape, and it has thus been proposed to play key cellular functions. The dynamics of PA in cells and in subcellular compartments, however, remains an open question. The recent generation of fluorescent probes for PA, by fusing GFP to PA-binding domains, has provided direct evidence for PA dynamics in different intracellular compartments. Here, three PA sensors were characterizedin vitro,and their preferences for different PA species in particular lipidic environments were compared. In addition, the localization of PA in macrophages during frustrated phagocytosis was examined using these PA sensors and was combined with a lipidomic analysis of PA in intracellular compartments. The results indicate that the PA sensors display some preferences for specific PA species, depending on the lipid environment, and the localization study in macrophages revealed the complexity of intracellular PA dynamics.
authors.affiliation: From the Institut des Neurosciences Cellulaires et Intégratives (INCI), UPR-3212 CNRS and Université de Strasbourg, 5 Rue Blaise Pascal, 67084 Strasbourg.
authors.forename: Nawal
authors.initials: N
authors.lastname: Kassas
authors.affiliation: From the Institut des Neurosciences Cellulaires et Intégratives (INCI), UPR-3212 CNRS and Université de Strasbourg, 5 Rue Blaise Pascal, 67084 Strasbourg.
authors.forename: Emeline
authors.initials: E
authors.lastname: Tanguy
authors.affiliation: From the Institut des Neurosciences Cellulaires et Intégratives (INCI), UPR-3212 CNRS and Université de Strasbourg, 5 Rue Blaise Pascal, 67084 Strasbourg.
authors.forename: Tamou
authors.initials: T
authors.lastname: Thahouly
authors.affiliation: the Laboratoire de Biogénèse Membranaire, UMR-5200 CNRS, Plateforme Métabolome, Université de Bordeaux, 33883 Villenave D'Ornon, and.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Fouillen
authors.affiliation: the Plateforme Métabolomique, Institut de Biologie Moléculaire des Plantes, UPR-2357 CNRS and Université de Strasbourg, Institut de Botanique, 28 Rue Goethe, 67083 Strasbourg, France.
authors.forename: Dimitri
authors.initials: D
authors.lastname: Heintz
authors.affiliation: From the Institut des Neurosciences Cellulaires et Intégratives (INCI), UPR-3212 CNRS and Université de Strasbourg, 5 Rue Blaise Pascal, 67084 Strasbourg.
authors.forename: Sylvette
authors.initials: S
authors.lastname: Chasserot-Golaz
authors.affiliation: From the Institut des Neurosciences Cellulaires et Intégratives (INCI), UPR-3212 CNRS and Université de Strasbourg, 5 Rue Blaise Pascal, 67084 Strasbourg.
authors.forename: Marie-France
authors.initials: MF
authors.lastname: Bader
authors.affiliation: From the Institut des Neurosciences Cellulaires et Intégratives (INCI), UPR-3212 CNRS and Université de Strasbourg, 5 Rue Blaise Pascal, 67084 Strasbourg.
authors.forename: Nancy J
authors.initials: NJ
authors.lastname: Grant
authors.affiliation: From the Institut des Neurosciences Cellulaires et Intégratives (INCI), UPR-3212 CNRS and Université de Strasbourg, 5 Rue Blaise Pascal, 67084 Strasbourg, vitalen@unistra.fr.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Vitale
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D002462:Cell Membrane / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D008055:Lipids / Q000032:analysis; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D010587:Phagocytosis / Q000502:physiology*; D010712:Phosphatidic Acids / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D015290:Second Messenger Systems
chemical_list: D008055:Lipids; D010712:Phosphatidic Acids; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins
keywords: Phospholipase D; biosensor; fatty acid; lipid signaling; phagocytosis; phosphatidic acid