title: One-pot native chemical ligation by combination of two orthogonal thioester precursors.
abstract: We developed a one-pot peptide ligation method using two orthogonal thioester precursors and a protecting group for the ligation reaction between Asp and Cys. Combination of the two precursors facilitated the one-pot operation and yielded the entire polypeptide. The usefulness of this method was successfully demonstrated by the total synthesis of histone H4.
authors.affiliation: Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan. hojo@protein.osaka-u.ac.jp.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Asahina
authors.affiliation: Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan. hojo@protein.osaka-u.ac.jp.
authors.forename: Toru
authors.initials: T
authors.lastname: Kawakami
authors.affiliation: Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan. hojo@protein.osaka-u.ac.jp.
authors.forename: Hironobu
authors.identifier: http://orcid.org/0000-0003-0916-1233
authors.initials: H
authors.lastname: Hojo