title: Quality Control by Isoleucyl-tRNA Synthetase of Bacillus subtilis Is Required for Efficient Sporulation.
abstract: Isoleucyl-tRNA synthetase (IleRS) is an aminoacyl-tRNA synthetase whose essential function is to aminoacylate tRNAIlewith isoleucine. Like some other aminoacyl-tRNA synthetases, IleRS can mischarge tRNAIleand correct this misacylation through a separate post-transfer editing function. To explore the biological significance of this editing function, we created a ileS(T233P) mutant of Bacillus subtilis that allows tRNAIlemischarging while retaining wild-type Ile-tRNAIlesynthesis activity. As seen in other species defective for aminoacylation quality control, the growth rate of the ileS(T233P) strain was not significantly different from wild-type. When the ileS(T233P) strain was assessed for its ability to promote distinct phenotypes in response to starvation, the ileS(T233P) strain was observed to exhibit a significant defect in formation of environmentally resistant spores. The sporulation defect ranged from 3-fold to 30-fold and was due to a delay in activation of early sporulation genes. The loss of aminoacylation quality control in the ileS(T233P) strain resulted in the inability to compete with a wild-type strain under selective conditions that required sporulation. These data show that the quality control function of IleRS is required in B. subtilis for efficient sporulation and suggests that editing by aminoacyl-tRNA synthetases may be important for survival under starvation/nutrient limitation conditions.
authors.affiliation: Department of Microbiology, Immunology and Molecular Genetics University of California, Los Angeles, California 90095, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Kermgard
authors.affiliation: Department of Microbiology, Immunology and Molecular Genetics University of California, Los Angeles, California 90095, USA.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Microbiology, Immunology and Molecular Genetics University of California, Los Angeles, California 90095, USA.|Technische Universität Braunschweig, Institut of Microbiology, Braunschweig, Germany.
authors.forename: Annika-Marisa
authors.initials: AM
authors.lastname: Michel
authors.affiliation: Ohio State Biochemistry Program, Ohio State University, Columbus, Ohio 43210, USA.
authors.forename: Rachel
authors.initials: R
authors.lastname: Simari
authors.affiliation: Department of Microbiology, Immunology and Molecular Genetics University of California, Los Angeles, California 90095, USA.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Wong
authors.affiliation: Ohio State Biochemistry Program, Ohio State University, Columbus, Ohio 43210, USA.|Department of Microbiology, Ohio State University, Columbus, Ohio 43210, USA.|Center for RNA Biology, Ohio State University, Columbus, Ohio 43210, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Ibba
authors.affiliation: Department of Microbiology, Immunology and Molecular Genetics University of California, Los Angeles, California 90095, USA.|Molecular Biology Institute, University of California, Los Angeles, California 90095, USA.
authors.forename: Beth A
authors.initials: BA
authors.lastname: Lazazzera
mesh_terms: D000483:Alleles; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001412:Bacillus subtilis / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects; D024881:Drug Resistance, Bacterial; D015964:Gene Expression Regulation, Bacterial; D007533:Isoleucine-tRNA Ligase / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation; D013171:Spores, Bacterial; D013379:Substrate Specificity; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C080950:Spo0A protein, Bacillus subtilis; D014157:Transcription Factors; D007533:Isoleucine-tRNA Ligase