title: Effects of LncRNA-HOST2 on cell proliferation, migration, invasion and apoptosis of human hepatocellular carcinoma cell line SMMC-7721.
abstract: The present study explored the effect of long non-coding RNA-human ovarian cancer-specific transcript 2 (LncRNA-HOST2) on cell proliferation, migration, invasion and apoptosis of human hepatocellular carcinoma (HCC) cell line SMMC-7721. HCC tissues and adjacent normal tissues from 162 HCC patients were collected. The HCC cell lines were assigned into the control group (regular culture), negative control (NC) group (transfected with siRNA) and experimental group (transfected with Lnc-HOST2 siRNA). Quantitative real-time PCR (qRT-PCR) was used to detect the expression of LncRNA-HOST2. Cell proliferation was detected by CCK-8 and colony-forming assays, cell apoptosis by flow cytometry and cell migration by Scratch test. Transwell assay was used to evaluate cell migration and invasion abilities. LncRNA-HOST2 expression in the HCC tissues increased 2-10 times than that in the adjacent normal tissues. Compared with the HL-7702 cell line, LncRNA-HOST2 expression in HepG2, SMMC-7721 and Huh7 cell lines was all up-regulated, but the SMMC-7721 cell had the highest Lnc-HOST2 expression. The LncRNA-HOST2 expression in the experimental group was down-regulated as compared with the control and NC groups. In comparison with the control and NC groups, cloned cells reduced, cell apoptosis increased, clone-forming ability weakened and inhibitory rate of colony formation increased in the experimental group. The cells migrating and penetrating into the transwell chamber were fewer in the experimental group than those in the control and NC groups. The experimental group exhibited slow wound healing and decreased cell migration area after 48 h. These findings indicate that LncRNA-HOST2 can promote cell proliferation, migration and invasion and inhibit cell apoptosis in human HCC cell line SMMC-7721.
authors.affiliation: Department of Hepatobiliary Surgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050051, P.R. China.
authors.forename: Run-Tian
authors.initials: RT
authors.lastname: Liu
authors.affiliation: Department of Hepatobiliary Surgery, The Third Hospital of Hebei Medical University, Shijiazhuang 050051, P.R. China jinglin_cao@163.com.
authors.forename: Jing-Lin
authors.initials: JL
authors.lastname: Cao
authors.affiliation: Department of Hepatobiliary Surgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050051, P.R. China.
authors.forename: Chang-Qing
authors.initials: CQ
authors.lastname: Yan
authors.affiliation: Department of Hepatobiliary Surgery, The Third Hospital of Hebei Medical University, Shijiazhuang 050051, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Medical Examination Center, The Second Hospital of Hebei Medical University, Shijiazhuang 050051, P.R. China.
authors.forename: Cong-Jing
authors.initials: CJ
authors.lastname: An
authors.affiliation: Department of Hepatobiliary Surgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050051, P.R. China.
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Lv
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D009361:Neoplasm Invasiveness / Q000235:genetics*; D034622:RNA Interference / Q000502:physiology; D062085:RNA, Long Noncoding / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics
chemical_list: C000599552:LncRNA-HOST2, human; D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering
keywords: Hepatocellular carcinoma; Human ovarian cancer specific transcript 2; Long noncoding RNA; Migration; Proliferation; SMMC-7721 cell line