title: Anti-asthmatic activity of osthole in an ovalbumin-induced asthma murine model.
abstract: Osthole, an active coumarin extracted from the dried fruits of Cnidium monnieri (L.) Cusson, is known to possess a variety of pharmacological activities. In the present study, we investigated and illuminated the mechanisms underlying the protective effects of osthole in an experimental model of allergic asthma. Our results show that osthole treatment significantly reduced the OVA-induced increase in serum IgE and inflammatory cytokines (IL-4, IL-5, IL-13) in bronchoalveolar lavage fluid (BALF), and decreased the recruitment of inflammatory cells in BALF and the lung. It also effectively attenuated goblet cell hyperplasia and mucus overproduction in lung tissue. In addition, western blot analysis demonstrated that osthole blocked NF-κB activation, which may be associated with a reduction in inflammatory cytokine production. These data suggest that osthole attenuated OVA-induced allergic asthma inflammation by inhibiting NF-κB activation. The present study identified the molecular mechanisms of action of osthole, which support the potential pharmaceutical application of osthole treatment for asthma and other airway inflammation disorders.
authors.affiliation: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, People's Republic of China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, People's Republic of China.
authors.forename: Yunhe
authors.initials: Y
authors.lastname: Fu
authors.affiliation: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, People's Republic of China.
authors.forename: Zhengkai
authors.initials: Z
authors.lastname: Wei
authors.affiliation: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, People's Republic of China.
authors.forename: Xuexiu
authors.initials: X
authors.lastname: He
authors.affiliation: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, People's Republic of China.
authors.forename: Mingyu
authors.initials: M
authors.lastname: Shi
authors.affiliation: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, People's Republic of China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Kou
authors.affiliation: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, People's Republic of China.
authors.forename: Ershun
authors.initials: E
authors.lastname: Zhou
authors.affiliation: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, People's Republic of China.
authors.forename: Weijian
authors.initials: W
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, People's Republic of China.
authors.forename: Zhengtao
authors.initials: Z
authors.lastname: Yang
authors.affiliation: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, People's Republic of China. Electronic address: guochm1980@163.com.
authors.forename: Changming
authors.initials: C
authors.lastname: Guo
mesh_terms: D000818:Animals; D018927:Anti-Asthmatic Agents / Q000627:therapeutic use*; D001249:Asthma / Q000097:blood / Q000139:chemically induced* / Q000188:drug therapy*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D003374:Coumarins / Q000627:therapeutic use*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D007073:Immunoglobulin E / Q000097:blood; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D010047:Ovalbumin / Q000633:toxicity*
chemical_list: D018927:Anti-Asthmatic Agents; D003374:Coumarins; D016207:Cytokines; D016328:NF-kappa B; D007073:Immunoglobulin E; D010047:Ovalbumin; C046627:osthol
keywords: Allergic asthma; Inflammation; NF-κB; Osthole