title: Basophil-derived IL-6 regulates TH17 cell differentiation and CD4 T cell immunity.
abstract: Basophils are rare, circulating granulocytes proposed to be involved in T helper (TH) type 2 immunity, mainly through secretion of interleukin (IL)-4. In addition to IL-4, basophils produce IL-6 and tumor necrosis factor (TNF)-α in response to immunoglobulin E (IgE) crosslinking. Differentiation of TH17 cells requires IL-6 and transforming growth factor (TGF)-β, but whether basophils play a significant role in TH17 induction is unknown. Here we show a role for basophils in TH17 cell development by using in vitro T cell differentiation and in vivo TH17-mediated inflammation models. Bone marrow derived-basophils (BMBs) and splenic basophils produce significant amounts of IL-6 as well as IL-4 following stimulation with IgE crosslink or cholera toxin (CT). In addition, through IL-6 secretion, BMBs cooperate with dendritic cells to promote TH17 cell differentiation. In the TH17 lung inflammation model, basophils are recruited to the inflamed lungs following CT challenge, and TH17 responses are significantly reduced in the absence of basophils or IL-6. Furthermore, reconstitution with wild-type, but not IL-6-deficient, basophils restored CT-mediated lung inflammation. Lastly, basophil-deficient mice showed reduced phenotypes of TH17-dependent experimental autoimmune encephalomyelitis. Therefore, our results indicate that basophils are an important inducer of TH17 cell differentiation, which is dependent on IL-6 secretion.
authors.affiliation: Biomedical Science and Engineering Interdisciplinary Program, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Korea.|Graduate School of Medical Science and Engineering (GSMSE), Biomedical Research Center, KAIST Institute for the Bio-Century, KAIST, Daejeon, 34141, Korea.
authors.forename: Chae Min
authors.initials: CM
authors.lastname: Yuk
authors.affiliation: Biomedical Science and Engineering Interdisciplinary Program, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Korea.|Graduate School of Medical Science and Engineering (GSMSE), Biomedical Research Center, KAIST Institute for the Bio-Century, KAIST, Daejeon, 34141, Korea.
authors.forename: Hyeung Ju
authors.initials: HJ
authors.lastname: Park
authors.affiliation: K-herb Research Center, Korea Institute of Oriental Medicine (KIOM), Daejeon, 34054, Korea.
authors.forename: Bo-In
authors.initials: BI
authors.lastname: Kwon
authors.affiliation: Biomedical Science and Engineering Interdisciplinary Program, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Korea.|Graduate School of Medical Science and Engineering (GSMSE), Biomedical Research Center, KAIST Institute for the Bio-Century, KAIST, Daejeon, 34141, Korea.
authors.forename: Sang Joon
authors.initials: SJ
authors.lastname: Lah
authors.affiliation: Division of Life and Pharmaceutical Science, Ewha Womans University, Seoul, 03760, Korea.
authors.forename: Jun
authors.initials: J
authors.lastname: Chang
authors.affiliation: Department of Biochemistry and Molecular Biology, Korea University College of Medicine, Seoul, 02841, Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Department of Biochemistry and Molecular Biology, Korea University College of Medicine, Seoul, 02841, Korea.
authors.forename: Kyung-Mi
authors.initials: KM
authors.lastname: Lee
authors.affiliation: Biomedical Science and Engineering Interdisciplinary Program, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Korea.|Graduate School of Medical Science and Engineering (GSMSE), Biomedical Research Center, KAIST Institute for the Bio-Century, KAIST, Daejeon, 34141, Korea.
authors.forename: Su-Hyung
authors.initials: SH
authors.lastname: Park
authors.affiliation: Graduate School of Medical Science and Engineering (GSMSE), Biomedical Research Center, KAIST Institute for the Bio-Century, KAIST, Daejeon, 34141, Korea.|Division of Rheumatology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, 05505, Korea.
authors.forename: Seokchan
authors.initials: S
authors.lastname: Hong
authors.affiliation: Biomedical Science and Engineering Interdisciplinary Program, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Korea.|Graduate School of Medical Science and Engineering (GSMSE), Biomedical Research Center, KAIST Institute for the Bio-Century, KAIST, Daejeon, 34141, Korea.
authors.forename: Seung-Hyo
authors.initials: SH
authors.lastname: Lee
mesh_terms: D000818:Animals; D001491:Basophils / Q000276:immunology* / Q000378:metabolism*; D015415:Biomarkers; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000502:physiology*; D002454:Cell Differentiation / Q000276:immunology*; D002772:Cholera Toxin / Q000009:adverse effects; D016207:Cytokines / Q000096:biosynthesis; D004195:Disease Models, Animal; D007109:Immunity; D016130:Immunophenotyping; D007249:Inflammation / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D015850:Interleukin-6 / Q000172:deficiency / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D058504:Th17 Cells / Q000166:cytology* / Q000502:physiology*
chemical_list: D015415:Biomarkers; D016207:Cytokines; D015850:Interleukin-6; D002772:Cholera Toxin