title: Luminescent Metal-Organic-Framework-Based Label-Free Assay of Polyphenol Oxidase with Fluorescent Scan.
abstract: Metal-organic frameworks (MOFs) are emerging in recent years as a kind of versatile fluorescent sensing materials, but their application to enzyme assays has rarely been studied. Here, the first example of a MOF-based label-free enzyme assay system is reported. A luminescent MOF was synthesized and applied to the activity analysis of polyphenol oxidase (PPO). With its distinct responses to the phenolic substrate and o-quinone product, this MOF could transduce the extent of PPO-catalyzed oxidation to fluorescence signal and enable the real-time monitoring of this reaction. Wide substrate adaptability and high sensitivity (detection limit=0.00012 U mL-1) were exhibited by this method, which meets the requirement of common bioanalysis. Interestingly, by the comparison with molecular capturing reagents, the heterogeneous nature of this MOF-based assay effectively preventing the interaction with the enzyme was proven, thus ensuring the authenticity of results.
authors.affiliation: College of Chemistry, and Key Laboratory of Advanced Energy Materials Chemistry, (Ministry of Education), Nankai University, Tianjin, 300071, P. R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Chemistry, and Key Laboratory of Advanced Energy Materials Chemistry, (Ministry of Education), Nankai University, Tianjin, 300071, P. R. China.
authors.forename: An
authors.initials: A
authors.lastname: Guo
authors.affiliation: College of Chemistry, and Key Laboratory of Advanced Energy Materials Chemistry, (Ministry of Education), Nankai University, Tianjin, 300071, P. R. China.
authors.forename: Lan
authors.initials: L
authors.lastname: Chang
authors.affiliation: College of Chemistry, and Key Laboratory of Advanced Energy Materials Chemistry, (Ministry of Education), Nankai University, Tianjin, 300071, P. R. China.
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Li
authors.affiliation: College of Chemistry, and Key Laboratory of Advanced Energy Materials Chemistry, (Ministry of Education), Nankai University, Tianjin, 300071, P. R. China.
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Ruan
mesh_terms: D000363:Agaricales / Q000201:enzymology; D055162:Biocatalysis; D004156:Catechol Oxidase / Q000378:metabolism*; D057075:Enzyme Assays; D008024:Ligands; D057230:Limit of Detection; D000073396:Metal-Organic Frameworks / Q000737:chemistry*; D010084:Oxidation-Reduction; D011809:Quinones / Q000737:chemistry / Q000378:metabolism; D011198:Solanum tuberosum / Q000201:enzymology; D013050:Spectrometry, Fluorescence; D013379:Substrate Specificity
chemical_list: D008024:Ligands; D000073396:Metal-Organic Frameworks; D011809:Quinones; D004156:Catechol Oxidase
keywords: enzyme assay; fluorescence; metal-organic frameworks; polyphenol oxidase; sensors