title: Non-autonomous role of Cdc42 in cell-cell communication during collective migration.
abstract: Collective cell migration is involved in numerous processes both physiological, such as embryonic development, and pathological such as metastasis. Compared to single cell migration, collective motion requires cell behaviour coordination through an as-yet poorly understood but critical cell-cell communication mechanism. Using Drosophila border cell migration, we show here that the small Rho GTPase Cdc42 regulates cell-cell communication. Indeed, we demonstrate that Cdc42 controls protrusion formation in a cell non-autonomous manner. Moreover, we found that the endocytic small GTPase Rab11, controls Cdc42 localisation to the periphery of migrating border cell clusters. Accordingly, over-expression of Cdc42 in border cells rescues the loss of Rab11 function. In addition, we showed that Cdc42 acts upstream of Moesin, a cytoskeletal regulator known to function downstream of rab11. Thus, our study positions Cdc42 as a new key player in cell-cell communication, acting downstream of Rab11.
authors.affiliation: LBCMCP, Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, France. Electronic address: nathalie.colombie@univ-tlse3.fr.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Colombié
authors.affiliation: LBCMCP, Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Choesmel-Cadamuro
authors.affiliation: LBCMCP, Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, France.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Series
authors.affiliation: Institute for Research in Immunology and Cancer, University of Montréal, Montréal, QC, Canada H3C 3J7.
authors.forename: Gregory
authors.initials: G
authors.lastname: Emery
authors.affiliation: LBCMCP, Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, France.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Wang
authors.affiliation: LBCMCP, Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, France; INSERM, U1048, U1048, I2MC and Université Toulouse III, Toulouse, F-31300 France.. Electronic address: damien.ramel@inserm.fr.
authors.forename: Damien
authors.initials: D
authors.lastname: Ramel
mesh_terms: D000818:Animals; D002450:Cell Communication; D002465:Cell Movement; D022081:Cell Surface Extensions / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology* / Q000378:metabolism*; D004705:Endocytosis; D019204:GTP-Binding Proteins / Q000378:metabolism*; D008954:Models, Biological
chemical_list: C090145:Cdc42 protein, Drosophila; D029721:Drosophila Proteins; D019204:GTP-Binding Proteins
keywords: Cdc42; Cell-cell communication; Collective cell migration; Endocytosis