title: Association ofCDKN2B-AS1rs1333049 with Brain Diseases: A Case-control Study and a Meta-analysis.
abstract: OBJECTIVE
  CDKN2B-AS1polymorphisms were shown to associate with the risk of stroke in European. The goal of this study was to evaluate the contribution ofCDKN2B-AS1rs1333049 to the risk of hemorrhagic stroke (HS) and brain tumor (BT) in Han Chinese.
  
  
  METHODS
  A total of 142 HSs, 115 BTs, and 494 controls were included in the current association study. The genotyping test was performed using the melting temperature shift method.
  
  
  RESULTS
  We failed to validate the association ofCDKN2B-AS1rs1333049 with the risk of brain disease. Significantly higher levels of low-density lipoprotein cholesterol (LDL-C) (p=0.027), high-density lipoprotein cholesterol (HDL-C) (p＜0.001) and total cholesterol (TC) (p＜0.001) were found in HSs in the genotype GG/GC carriers, but not the genotype CC carriers (p＞0.05). The meta-analysis of 10 studies among 133,993 individuals concluded that rs1333049 ofCDKN2B-AS1gene was likely to increase a 16% incidence rate of cerebrovascular disease (CD) among various populations (odds ratio 1.16, 95% confidence interval 1.08-1.25;p＜0.0001, random-effect method).
  
  
  CONCLUSIONS
  Our case-control study identified rs1333049 genotypes showed different association with the concentration of the LDL-C, HDL-C and TC in the HS patients. Meta-analysis supported the association between rs1333049 and CD risk in various populations, although we were unable to observe association between rs1333049 and the risk of HSs in Han Chinese.
authors.affiliation: Department of Neurosurgery, Ningbo First Hospital, Ningbo Hospital of Zhejiang University, Ningbo, China.
authors.forename: Jikuang
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, Ninghai First Hospital, Ninghai, China.
authors.forename: Xizheng
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Neurosurgery, Ningbo First Hospital, Ningbo Hospital of Zhejiang University, Ningbo, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Nie
authors.affiliation: Department of Neurosurgery, Ningbo First Hospital, Ningbo Hospital of Zhejiang University, Ningbo, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Neurosurgery, Ningbo First Hospital, Ningbo Hospital of Zhejiang University, Ningbo, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Neurosurgery, Ningbo First Hospital, Ningbo Hospital of Zhejiang University, Ningbo, China.
authors.forename: Keqin
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Ningbo First Hospital, Ningbo Hospital of Zhejiang University, Ningbo, China.
authors.forename: Tiefeng
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Ningbo First Hospital, Ningbo Hospital of Zhejiang University, Ningbo, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Huang
keywords: CDKN2BAS1; Cerebrovascular disease; Hemorrhagic stroke; Lipid concentration; rs1333049