title: The deubiquitinating enzyme Usp5 regulates Notch and RTK signaling during Drosophila eye development.
abstract: Usp5 belongs to the USP family of deubiquitinating enzymes (DUBs), which comprises the largest class of DUBs. We previously reported that loss of Usp5 impairs development of photoreceptors in Drosophila eyes, although the detailed mechanism remained unclear. In the present study, we demonstrate that Usp5 regulates both Notch and receptor tyrosine kinase (RTK) signaling. Loss of Usp5 results in upregulation of Notch signaling and downregulation of RTK signaling, leading to impaired photoreceptor development. Moreover, genetic rescue experiments with the DNA binding protein Suppressor of Hairless or Notch RNAi indicate that they mediate the regulation of RTK signaling by Usp5. The present study provides mechanistic insight into how Usp5 regulates photoreceptor differentiation by Notch and RTK signaling in the Drosophila eye.
authors.affiliation: School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical University, Zhejiang, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Ling
authors.affiliation: Taizhou Hospital of Zhejiang Province, Wenzhou Medical University, Linhai, Zhejiang, China.
authors.forename: Qinzhu
authors.initials: Q
authors.lastname: Huang
authors.affiliation: School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical University, Zhejiang, China.
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Xu
authors.affiliation: School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical University, Zhejiang, China.
authors.forename: Yuxiao
authors.initials: Y
authors.lastname: Jin
authors.affiliation: School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical University, Zhejiang, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Feng
authors.affiliation: School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical University, Zhejiang, China.
authors.forename: Weijie
authors.initials: W
authors.lastname: Shi
authors.affiliation: School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical University, Zhejiang, China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Ye
authors.affiliation: School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical University, Zhejiang, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Lin
authors.affiliation: School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical University, Zhejiang, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Hou
authors.affiliation: School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical University, Zhejiang, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Lin
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D000072017:Deubiquitinating Enzymes / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism; D005123:Eye / Q000254:growth & development / Q000378:metabolism*; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D007814:Larva / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D018613:Microscopy, Confocal; D009154:Mutation; D017956:Photoreceptor Cells, Invertebrate / Q000378:metabolism; D034622:RNA Interference; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism; D018010:Receptors, Invertebrate Peptide / Q000235:genetics / Q000378:metabolism; D051880:Receptors, Notch / Q000235:genetics* / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics; D064570:Ubiquitin-Specific Proteases / Q000235:genetics* / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D005136:Eye Proteins; C497759:N protein, Drosophila; D018010:Receptors, Invertebrate Peptide; D051880:Receptors, Notch; D012097:Repressor Proteins; C088731:Su(H) protein, Drosophila; C066532:Egfr protein, Drosophila; D066246:ErbB Receptors; D020794:Receptor Protein-Tyrosine Kinases; C056737:sev protein, Drosophila; C000593006:USP5 protein, Drosophila; D000072017:Deubiquitinating Enzymes; D064570:Ubiquitin-Specific Proteases
keywords: 
  EGFR
  ; 
  RTK
  ; Notch; Su(H); Yan; deubiquitinating enzymes; photoreceptors