title: Approaches to Study Light Effects on Brassinosteroid Sensitivity.
abstract: Light perception and hormone signaling in plants are likely connected at multiple points. Light conditions, perceived by photoreceptors, control plant responses by altering hormone concentration, tissue sensitivity, or a combination of both. Whereas it is relatively straightforward to assess the light effects on hormone levels, hormone sensitivity is subjected to interpretation. In Arabidopsis thaliana seedlings, hypocotyl length is strongly affected by light conditions. As hypocotyl elongation also depends on brassinosteroids (BRs), assaying this response provides a valuable and easy way to measure the responsiveness of seedlings to BRs and the impact of light. We describe a simple protocol to evaluate the responsiveness of hypocotyls to commercial BRs and/or BR inhibitors under a range of light conditions. These assays can be used to establish whether light affects BR sensitivity or whether BRs affect light sensitivity. Overall, our protocol can be easily applied for deetiolation (under polychromatic or monochromatic light) and simulated shade treatments combined with BR treatments.
authors.affiliation: Centre for Research in Agricultural Genomics (CRAG), Consejo Superior de Investigaciones Científicas-Institut de Recerca i Tecnologia Agroalimentàries-Universitat Autonòma de Barcelona-Universitat de Barcelona (CSIC-IRTA-UAB- UB), 08193, Barcelona, Spain.
authors.forename: Sandi
authors.initials: S
authors.lastname: Paulišić
authors.affiliation: Centre for Research in Agricultural Genomics (CRAG), Consejo Superior de Investigaciones Científicas-Institut de Recerca i Tecnologia Agroalimentàries-Universitat Autonòma de Barcelona-Universitat de Barcelona (CSIC-IRTA-UAB- UB), 08193, Barcelona, Spain.
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Molina-Contreras
authors.affiliation: Centre for Research in Agricultural Genomics (CRAG), Consejo Superior de Investigaciones Científicas-Institut de Recerca i Tecnologia Agroalimentàries-Universitat Autonòma de Barcelona-Universitat de Barcelona (CSIC-IRTA-UAB- UB), 08193, Barcelona, Spain.
authors.forename: Irma
authors.initials: I
authors.lastname: Roig-Villanova
authors.affiliation: Centre for Research in Agricultural Genomics (CRAG), Consejo Superior de Investigaciones Científicas-Institut de Recerca i Tecnologia Agroalimentàries-Universitat Autonòma de Barcelona-Universitat de Barcelona (CSIC-IRTA-UAB- UB), 08193, Barcelona, Spain. jaume.martinez@cragenomica.es.|, Institució Catalana de Recerca i Estudis Avançats (ICREA), 08010, Barcelona, Spain. jaume.martinez@cragenomica.es.
authors.forename: Jaime F
authors.initials: JF
authors.lastname: Martínez-García
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000254:growth & development / Q000528:radiation effects*; D060406:Brassinosteroids / Q000494:pharmacology*; D005659:Fungicides, Industrial / Q000494:pharmacology; D018546:Hypocotyl / Q000187:drug effects / Q000254:growth & development / Q000528:radiation effects*; D008027:Light; D055537:Light Signal Transduction / Q000502:physiology*; D010507:Periodicity; D010937:Plant Growth Regulators / Q000494:pharmacology*; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development / Q000528:radiation effects; D013260:Steroids, Heterocyclic / Q000494:pharmacology*; D014230:Triazoles / Q000494:pharmacology
chemical_list: D060406:Brassinosteroids; D005659:Fungicides, Industrial; D010937:Plant Growth Regulators; D013260:Steroids, Heterocyclic; D014230:Triazoles; C045950:propiconazole; C023623:brassinolide
keywords: Dark; Deetiolation; Hormone sensitivity; Hypocotyl length; Monochromatic light; Photoreceptors; Shade