title: Bioleaching of electronic waste using bacteria isolated from the marine sponge Hymeniacidon heliophila (Porifera).
abstract: The bacteria isolated from Hymeniacidon heliophila sponge cells showed bioleaching activity. The most active strain, Hyhel-1, identified as Bacillus sp., was selected for bioleaching tests under two different temperatures, 30°C and 40°C, showing rod-shaped cells and filamentous growth, respectively. At 30°C, the bacteria secreted substances which linked to the leached copper, and at 40°C metallic nanoparticles were produced inside the cells. In addition, infrared analysis detected COOH groups and linear peptides in the tested bacteria at both temperatures. The Hyhel-1 strain in presence of electronic waste (e-waste) induced the formation of crust, which could be observed due to bacteria growing on the e-waste fragment. SEM-EDS measurements showed that the bacterial net surface was composed mostly of iron (16.1% w/w), while a higher concentration of copper was observed in the supernatant (1.7% w/w) and in the precipitated (49.8% w/w). The substances linked to copper in the supernatant were sequenced by MALDI-TOF-ms/ms and identified as macrocyclic surfactin-like peptides, similar to the basic sequence of Iturin, a lipopeptide from Bacillus subtilis. Finally, the results showed that Hyhel-1 is a bioleaching bacteria and cooper nanoparticles producer and that this bacteria could be used as a copper recovery tool from electronic waste.
authors.affiliation: LSCP, Chemical Engineering Department, University of São Paulo (USP), Brazil. Electronic address: isoquir@gmail.com.
authors.forename: Enrique E
authors.initials: EE
authors.lastname: Rozas
authors.affiliation: LSCP, Chemical Engineering Department, University of São Paulo (USP), Brazil.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Mendes
authors.affiliation: LSCP, Chemical Engineering Department, University of São Paulo (USP), Brazil.
authors.forename: Claudio A O
authors.initials: CA
authors.lastname: Nascimento
authors.affiliation: LAREX, Chemical Engineering Department, University of São Paulo (USP), Brazil.
authors.forename: Denise C R
authors.initials: DC
authors.lastname: Espinosa
authors.affiliation: Vale Institute of Technology, Belém, PA, Brazil.
authors.forename: Renato
authors.initials: R
authors.lastname: Oliveira
authors.affiliation: Vale Institute of Technology, Belém, PA, Brazil.
authors.forename: Guilherme
authors.initials: G
authors.lastname: Oliveira
authors.affiliation: Departamento de Fisiologia Geral, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Marcio R
authors.initials: MR
authors.lastname: Custodio
mesh_terms: D000818:Animals; D001407:Bacillus / Q000302:isolation & purification* / Q000502:physiology / Q000648:ultrastructure; D003300:Copper / Q000302:isolation & purification* / Q000378:metabolism; D059029:Electronic Waste; D011161:Porifera / Q000382:microbiology*; D059027:Recycling / Q000379:methods*
chemical_list: D003300:Copper
keywords: Bacillus; Bioleaching; E-waste; Nanoparticles; Sponge cell