title: Key genes expressed in different stages of spinal cord ischemia/reperfusion injury.
abstract: The temporal expression of microRNA after spinal cord ischemia/reperfusion injury is not yet fully understood. In the present study, we established a model of spinal cord ischemia in Sprague-Dawley rats by clamping the abdominal aorta for 90 minutes, before allowing reperfusion for 24 or 48 hours. A sham-operated group underwent surgery but the aorta was not clamped. The damaged spinal cord was removed for hematoxylin-eosin staining and RNA extraction. Neuronal degeneration and tissue edema were the most severe in the 24-hour reperfusion group, and milder in the 48-hour reperfusion group. RNA amplification, labeling, and hybridization were used to obtain the microRNA expression profiles of each group. Bioinformatics analysis confirmed four differentially expressed microRNAs (miR-22-3p, miR-743b-3p, miR-201-5p and miR-144-5p) and their common target genes (Tmem69 and Cxcl10). Compared with the sham group, miR-22-3p was continuously upregulated in all three ischemia groups but was highest in the group with no reperfusion, whereas miR-743b-3p, miR-201-5p and miR-144-5p were downregulated in the three ischemia groups. We have successfully identified the key genes expressed at different stages of spinal cord ischemia/reperfusion injury, which provide a reference for future investigations into the mechanism of spinal cord injury.
authors.affiliation: Department of Orthopaedics, the Second Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Jian-An
authors.initials: JA
authors.lastname: Li
authors.affiliation: Department of Orthopaedics, the Second Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Chun-Fang
authors.initials: CF
authors.lastname: Zan
authors.affiliation: Department of Orthopaedics, the Second Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xia
authors.affiliation: Department of Orthopaedics, the Second Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Chang-Jun
authors.initials: CJ
authors.lastname: Zheng
authors.affiliation: Department of Orthopaedics, the Second Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Zhi-Ping
authors.initials: ZP
authors.lastname: Qi
authors.affiliation: Department of Orthopaedics, the Second Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Chun-Xu
authors.initials: CX
authors.lastname: Li
authors.affiliation: Department of Orthopaedics, the Second Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, the Second Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Ting-Ting
authors.identifier: 0000-0001-9010-2147
authors.initials: TT
authors.lastname: Hou
authors.affiliation: Department of Orthopaedics, the Second Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Xiao-Yu
authors.identifier: 0000-0001-9388-3794
authors.initials: XY
authors.lastname: Yang
keywords: Cxcl10; Tmem69; bioinformatics; ischemia/reperfusion injury; mRNA; microRNA; microRNA arrays; nerve regeneration; neural regeneration; spinal cord injury; transcriptome