title: Platelet-functionalized three-dimensional poly-ε-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors.
abstract: Bone and cartilage are tissues of a three-dimensional (3D) nature. Therefore, scaffolds for their regeneration should support cell infiltration and growth in all 3 dimensions. To fulfill such a requirement, the materials should possess large, open pores. Centrifugal spinning is a simple method for producing 3D fibrous scaffolds with large and interconnected pores. However, the process of bone regeneration is rather complex and requires additional stimulation by active molecules. In the current study, we introduced a simple composite scaffold based on platelet adhesion to poly-ε-caprolactone 3D fibers. Platelets were used as a natural source of growth factors and cytokines active in the tissue repair process. By immobilization in the fibrous scaffolds, their bioavailability was prolonged. The biological evaluation of the proposed system in the MG-63 model showed improved metabolic activity, proliferation and alkaline phosphatase activity in comparison to nonfunctionalized fibrous scaffold. In addition, the response of cells was dose dependent with improved biocompatibility with increasing platelet concentration. The results demonstrated the suitability of the system for bone tissue.
authors.affiliation: Indoor Environmental Quality, University Center for Energy Efficient Buildings, Czech Technical University in Prague, Buštěhrad; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic.
authors.forename: Michala
authors.initials: M
authors.lastname: Rampichová
authors.affiliation: Indoor Environmental Quality, University Center for Energy Efficient Buildings, Czech Technical University in Prague, Buštěhrad.
authors.forename: Matej
authors.initials: M
authors.lastname: Buzgo
authors.affiliation: Indoor Environmental Quality, University Center for Energy Efficient Buildings, Czech Technical University in Prague, Buštěhrad; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic.
authors.forename: Andrea
authors.initials: A
authors.lastname: Míčková
authors.affiliation: Laboratory of Tissue Engineering, Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic.
authors.forename: Karolína
authors.initials: K
authors.lastname: Vocetková
authors.affiliation: Laboratory of Tissue Engineering, Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic.
authors.forename: Věra
authors.initials: V
authors.lastname: Sovková
authors.affiliation: Laboratory of Tissue Engineering, Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic.
authors.forename: Věra
authors.initials: V
authors.lastname: Lukášová
authors.affiliation: Laboratory of Tissue Engineering, Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Filová
authors.affiliation: Laboratory of Tissue Engineering, Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic.
authors.forename: Franco
authors.initials: F
authors.lastname: Rustichelli
authors.affiliation: Indoor Environmental Quality, University Center for Energy Efficient Buildings, Czech Technical University in Prague, Buštěhrad; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic.
authors.forename: Evžen
authors.initials: E
authors.lastname: Amler
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D016503:Drug Delivery Systems / Q000379:methods*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000008:administration & dosage* / Q000494:pharmacology*; D007700:Kinetics; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D010012:Osteogenesis / Q000187:drug effects; D010973:Platelet Adhesiveness / Q000187:drug effects; D011091:Polyesters / Q000737:chemistry* / Q000494:pharmacology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D011091:Polyesters; C016240:polycaprolactone; D000469:Alkaline Phosphatase
keywords: 3D scaffold; PCL; centrifugal spinning; cytokines; growth factors; platelets