title: Neuroprotection against hydrogen peroxide-induced toxicity by Dictyophora echinovolvata polysaccharide via inhibiting the mitochondria-dependent apoptotic pathway.
abstract: Neuronal apoptosis caused by toxic stimuli such as oxidative stress is believed to be one of the major reasons in the pathologenesis of neurodegenerative diseases. In the current study, the neuroprotective effects of the crude polysaccharide fraction of edible Dictyophora echinovolvata (DEVP) against H2O2-induced cytotoxicity were investigated using PC12 cells. Following exposure of PC12 cells to 750μM H2O2, a significant reduction in cell viability and the number of FDA-stained viable neurons as well as an increase in the number of PI-stained dead cells were observed. Furthermore, H2O2treatment significantly upregulated the protein expression of Bax, cleaved caspases 3 and cytosolic cytochrome c, and down-regulated Bcl-2 levels. 2h pre-treatment with VP reversed these changes caused by H2O2, including inhibiting neuronal loss and decreasing Bax, cleaved caspases 3 and cytosolic cytochrome c levels, as well as increasing Bcl-2 levels. These results taken together demonstrated that DEVP provided a substantial neuroprotection against H2O2-induced toxicity in PC12 cells, at least partly through inhibiting the mitochondrial apoptotic pathway. These findings suggested that DEVP might be a potential candidate for further preclinical study for preventing neurodegenerative diseases in which oxidative stress and apoptosis are involved.
authors.affiliation: Shenzhen Research Institute of The Hong Kong Polytechnic University, State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation), Shenzhen, Guangdong, People's Republic of China; Shenzhen Key Laboratory of Food Biological Safety Control, Shenzhen, Guangdong, People's Republic of China; Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, People's Republic of China.
authors.forename: Wen-Xuan
authors.initials: WX
authors.lastname: Yu
authors.affiliation: Soil and Fertilizer Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, People's Republic of China.
authors.forename: Chen-Qiang
authors.initials: CQ
authors.lastname: Lin
authors.affiliation: Department of Neurology, Linzi Maternal & Child Health Hospital of Zibo, Zibo, Shandong, People's Republic of China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Soil and Fertilizer Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, People's Republic of China. Electronic address: xinjianlin@163.net.
authors.forename: Xin-Jian
authors.initials: XJ
authors.lastname: Lin
authors.affiliation: Shenzhen Research Institute of The Hong Kong Polytechnic University, State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation), Shenzhen, Guangdong, People's Republic of China; Shenzhen Key Laboratory of Food Biological Safety Control, Shenzhen, Guangdong, People's Republic of China; Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, People's Republic of China. Electronic address: xiaoli.dong@polyu.edu.hk.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Dong
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001487:Basidiomycota / Q000737:chemistry*; D048430:Cell Shape / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000633:toxicity*; D042541:Intracellular Space / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D000066829:Neuroprotection / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology; D009498:Neurotoxins / Q000633:toxicity; D016716:PC12 Cells; D011134:Polysaccharides / Q000494:pharmacology*; D051381:Rats
chemical_list: D018696:Neuroprotective Agents; D009498:Neurotoxins; D011134:Polysaccharides; D006861:Hydrogen Peroxide
keywords: Apoptosis; Bax; Bcl-2; Mitochondrial-dependent; Neuroprotection; Oxidative stress