title: The Histone Chaperone NRP1 Interacts with WEREWOLF to ActivateGLABRA2in Arabidopsis Root Hair Development.
abstract: NUCLEOSOME ASSEMBLY PROTEIN1 (NAP1) defines an evolutionarily conserved family of histone chaperones and loss of function of theArabidopsis thalianaNAP1 family genesNAP1-RELATED PROTEIN1(NRP1) andNRP2causes abnormal root hair formation. Yet, the underlying molecular mechanisms remain unclear. Here, we show that NRP1 interacts with the transcription factor WEREWOLF (WER) in vitro and in vivo and enriches at theGLABRA2(GL2) promoter in a WER-dependent manner. Crystallographic analysis indicates that NRP1 forms a dimer via its N-terminal α-helix. Mutants of NRP1 that either disrupt the α-helix dimerization or remove the C-terminal acidic tail, impair its binding to histones and WER and concomitantly lead to failure to activateGL2transcription and to rescue thenrp1-1 nrp2-1mutant phenotype. Our results further demonstrate that WER-dependent enrichment of NRP1 at theGL2promoter is involved in local histone eviction and nucleosome loss in vivo. Biochemical competition assays imply that the association between NRP1 and histones may counteract the inhibitory effect of histones on the WER-DNA interaction. Collectively, our study provides important insight into the molecular mechanisms by which histone chaperones are recruited to target chromatin via interaction with a gene-specific transcription factor to moderate chromatin structure for proper root hair development.
authors.affiliation: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, International Associated Laboratory of CNRS-Fudan-HUNAU on Plant Epigenome Research, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 20043, P.R. China aiwudong@fudan.edu.cn majb@fudan.edu.cn zhu_yan@fudan.edu.cn.
authors.forename: Yan
authors.identifier: 0000-0001-7206-2612
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 20043, P.R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Rong
authors.affiliation: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, International Associated Laboratory of CNRS-Fudan-HUNAU on Plant Epigenome Research, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 20043, P.R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, International Associated Laboratory of CNRS-Fudan-HUNAU on Plant Epigenome Research, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 20043, P.R. China.
authors.forename: Baihui
authors.initials: B
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, International Associated Laboratory of CNRS-Fudan-HUNAU on Plant Epigenome Research, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 20043, P.R. China.
authors.forename: Nana
authors.initials: N
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, International Associated Laboratory of CNRS-Fudan-HUNAU on Plant Epigenome Research, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 20043, P.R. China.
authors.forename: Yue
authors.identifier: 0000-0002-6360-6857
authors.initials: Y
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, International Associated Laboratory of CNRS-Fudan-HUNAU on Plant Epigenome Research, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 20043, P.R. China.
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, International Associated Laboratory of CNRS-Fudan-HUNAU on Plant Epigenome Research, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 20043, P.R. China.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 20043, P.R. China.
authors.forename: Lina
authors.identifier: 0000-0002-6296-3668
authors.initials: L
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, International Associated Laboratory of CNRS-Fudan-HUNAU on Plant Epigenome Research, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 20043, P.R. China.|Institut de Biologie Moléculaire des Plantes, UPR2357 CNRS, Université de Strasbourg, 67084 Strasbourg Cédex, France.
authors.forename: Wen-Hui
authors.identifier: 0000-0001-7988-6382
authors.initials: WH
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 20043, P.R. China aiwudong@fudan.edu.cn majb@fudan.edu.cn zhu_yan@fudan.edu.cn.
authors.forename: Jinbiao
authors.identifier: 0000-0003-1860-9714
authors.initials: J
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, International Associated Laboratory of CNRS-Fudan-HUNAU on Plant Epigenome Research, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 20043, P.R. China aiwudong@fudan.edu.cn majb@fudan.edu.cn zhu_yan@fudan.edu.cn.
authors.forename: Aiwu
authors.identifier: 0000-0002-2075-4235
authors.initials: A
authors.lastname: Dong
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D019281:Dimerization; D018506:Gene Expression Regulation, Plant; D006657:Histones / Q000378:metabolism / Q000502:physiology; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D011401:Promoter Regions, Genetic
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C089991:GL2 protein, Arabidopsis; D006657:Histones; D018398:Homeodomain Proteins; C402111:WER protein, Arabidopsis; C551996:nodulin-related protein 1, Arabidopsis