title: Isolation and Characterization of a Phaseolus vulgaris Trypsin Inhibitor with Antiproliferative Activity on Leukemia and Lymphoma Cells.
abstract: A 17.5-kDa trypsin inhibitor was purified fromPhaseolus vulgariscv. "gold bean" with an isolation protocol including ion exchange chromatography on DEAE-cellulose (Diethylaminoethyl-cellulose), affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-sepharose (Sulfopropyl-sepharose), and gel filtration by FPLC (Fast protein liquid chromatography) on Superdex 75. It dose-dependently inhibited trypsin with an IC50value of 0.4 μM, and this activity was reduced in the presence of dithiothreitol in a dose- and time-dependent manner, signifying the importance of the disulfide linkage to the activity. It inhibited [methyl-³H] thymidine incorporation by leukemia L1210 cells and lymphoma MBL2 cells with an IC50value of 2.3 μM and 2.5 μM, respectively. The inhibitor had no effect on fungal growth and the activities of various viral enzymes when tested up to 100 μM.
authors.affiliation: State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, China. shuiyuemiaomiao@163.com.|Engineering Research Center of System-Nutrition, Beijing Research Institute for Nutritional Resources, Beijing 100069, China. shuiyuemiaomiao@163.com.
authors.forename: Miao
authors.initials: M
authors.lastname: Li
authors.affiliation: Institute of Plant Nutrition, Agricultural Resources and Environmental Science, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. liuqin_bio@hotmail.com.
authors.forename: Qin
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Engineering Research Center of System-Nutrition, Beijing Research Institute for Nutritional Resources, Beijing 100069, China. 15801453639@163.com.
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Engineering Research Center of System-Nutrition, Beijing Research Institute for Nutritional Resources, Beijing 100069, China. xingyunxing_127@163.com.
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.affiliation: State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, China. hxwang@cau.edu.cn.
authors.forename: Hexiang
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China. tzibunng@cuhk.edu.hk.
authors.forename: Tzi Bun
authors.initials: TB
authors.lastname: Ng
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003636:DEAE-Cellulose / Q000737:chemistry; D005658:Fungi / Q000254:growth & development / Q000378:metabolism; D006801:Humans; D007938:Leukemia / Q000473:pathology*; D008223:Lymphoma / Q000473:pathology*; D051379:Mice; D027805:Phaseolus / Q000201:enzymology*; D012639:Seeds / Q000201:enzymology; D012685:Sepharose / Q000737:chemistry; D014357:Trypsin / Q000378:metabolism*; D014361:Trypsin Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D014361:Trypsin Inhibitors; D012685:Sepharose; D003636:DEAE-Cellulose; D014357:Trypsin
keywords: leguminous seeds; leukemia L1210; lymphoma MBL2; purification