title: Antitumor magnetic hyperthermia induced by RGD-functionalized Fe3O4nanoparticles, in an experimental model of colorectal liver metastases.
abstract: This work reports important advances in the study of magnetic nanoparticles (MNPs) related to their application in different research fields such as magnetic hyperthermia. Nanotherapy based on targeted nanoparticles could become an attractive alternative to conventional oncologic treatments as it allows a local heating in tumoral surroundings without damage to healthy tissue. RGD-peptide-conjugated MNPs have been designed to specifically target αVβ3receptor-expressing cancer cells, being bound the RGD peptides by "click chemistry" due to its selectivity and applicability. The thermal decomposition of iron metallo-organic precursors yield homogeneous Fe3O4nanoparticles that have been properly functionalized with RGD peptides, and the preparation of magnetic fluids has been achieved. The nanoparticles were characterized by transmission electron microscopy (TEM), vibrating sample magnetometry (VSM), electron magnetic resonance (EMR) spectroscopy and magnetic hyperthermia. The nanoparticles present superparamagnetic behavior with very high magnetization values, which yield hyperthermia values above 500 W/g for magnetic fluids. These fluids have been administrated to rats, but instead of injecting MNP fluid directly into liver tumors, intravascular administration of MNPs in animals with induced colorectal tumors has been performed. Afterwards the animals were exposed to an alternating magnetic field in order to achieve hyperthermia. The evolution of an in vivo model has been described, resulting in a significant reduction in tumor viability.
authors.affiliation: Faculty of Science and Technology, University of the Basque Country, UPV/EHU, 48080, Bilbao, Spain.
authors.forename: Oihane K
authors.initials: OK
authors.lastname: Arriortua
authors.affiliation: Faculty of Science and Technology, University of the Basque Country, UPV/EHU, 48080, Bilbao, Spain.
authors.forename: Eneko
authors.initials: E
authors.lastname: Garaio
authors.affiliation: Faculty of Medicine and Nursing, University of the Basque Country, UPV/EHU, P.O. Box 644, 48080, Bilbao, Spain.
authors.forename: Borja
authors.initials: B
authors.lastname: Herrero de la Parte
authors.affiliation: Faculty of Science and Technology, University of the Basque Country, UPV/EHU, 48080, Bilbao, Spain; BCMaterials, Basque Center for Materials, Applications and Nanostructures, 48160, Derio, Spain.
authors.forename: Maite
authors.initials: M
authors.lastname: Insausti
authors.affiliation: Faculty of Science and Technology, University of the Basque Country, UPV/EHU, 48080, Bilbao, Spain; BCMaterials, Basque Center for Materials, Applications and Nanostructures, 48160, Derio, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Lezama
authors.affiliation: Faculty of Science and Technology, University of the Basque Country, UPV/EHU, 48080, Bilbao, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Plazaola
authors.affiliation: Faculty of Science and Technology, University of the Basque Country, UPV/EHU, 48080, Bilbao, Spain; BCMaterials, Basque Center for Materials, Applications and Nanostructures, 48160, Derio, Spain.
authors.forename: Jose Angel
authors.initials: JA
authors.lastname: García
authors.affiliation: José Mari Korta Center, University of the Basque Country, UPV/EHU, 20018 Donostia, Spain.
authors.forename: Jesús M
authors.initials: JM
authors.lastname: Aizpurua
authors.affiliation: José Mari Korta Center, University of the Basque Country, UPV/EHU, 20018 Donostia, Spain.
authors.forename: Maialen
authors.initials: M
authors.lastname: Sagartzazu
authors.affiliation: Faculty of Science and Technology, University of the Basque Country, UPV/EHU, 48080, Bilbao, Spain.
authors.forename: Mireia
authors.initials: M
authors.lastname: Irazola
authors.affiliation: Faculty of Science and Technology, University of the Basque Country, UPV/EHU, 48080, Bilbao, Spain.
authors.forename: Nestor
authors.initials: N
authors.lastname: Etxebarria
authors.affiliation: Faculty of Medicine and Nursing, University of the Basque Country, UPV/EHU, P.O. Box 644, 48080, Bilbao, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: García-Alonso
authors.affiliation: Galdakao Usansolo Hospital, 48960 Bizkaia, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Saiz-López
authors.affiliation: Galdakao Usansolo Hospital, 48960 Bizkaia, Spain.
authors.forename: José Javier
authors.initials: JJ
authors.lastname: Echevarria-Uraga
keywords: RGD functionalization; magnetic hyperthermia; magnetite nanoparticles; tumor targeting