title: The protein and neutral lipid composition of lipid droplets isolated from the fission yeast, Schizosaccharomyces pombe.
abstract: Lipid droplets consist of a core of neutral lipids surrounded by a phospholipid monolayer with bound proteins. Much of the information on lipid droplet function comes from proteomic and lipodomic studies that identify the components of droplets isolated from organisms throughout the phylogenetic tree. Here, we add to that important inventory by reporting lipid droplet factors from the fission yeast, Schizosaccharomyces pombe. Unique to this study was the fact that cells were cultured in three different environments: 1) late log growth phase in glucose-based media, 2) stationary phase in glucosebased media, and 3) late log growth phase in media containing oleic acid. We confirmed colocalization of major factors with lipid droplets using live-cell fluorescent microscopy. We also analyzed droplets from each of the three conditions for sterol ester (SE) and triacylglycerol (TAG) content, along with their respective fatty acid compositions. We identified a previously undiscovered lipid droplet protein, Vip1p, which affects droplet size distribution. The results provide further insight into the workings of these ubiquitous organelles.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996-2200, USA.
authors.forename: Alex
authors.initials: A
authors.lastname: Meyers
authors.affiliation: Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.
authors.forename: Karuna
authors.initials: K
authors.lastname: Chourey
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996-2200, USA.
authors.forename: Taylor M
authors.initials: TM
authors.lastname: Weiskittel
authors.affiliation: Department of Biochemistry, Cellular and Molecular Biology, University of Tennessee, Knoxville, TN, 37996, USA.
authors.forename: Susan
authors.initials: S
authors.lastname: Pfiffner
authors.affiliation: Department of Biochemistry, Cellular and Molecular Biology, University of Tennessee, Knoxville, TN, 37996, USA.|Advanced Microscopy and Imaging Center, University of Tennessee, Knoxville, TN, 37996, USA.
authors.forename: John R
authors.initials: JR
authors.lastname: Dunlap
authors.affiliation: Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Hettich
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996-2200, USA. pdalhaim@utk.edu.|Department of Biochemistry, Cellular and Molecular Biology, University of Tennessee, Knoxville, TN, 37996, USA. pdalhaim@utk.edu.|Institute of Biomedical Engineering, University of Tennessee, Knoxville, TN, 37996, USA. pdalhaim@utk.edu.
authors.forename: Paul
authors.initials: P
authors.lastname: Dalhaimer
mesh_terms: D003470:Culture Media / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis; D005947:Glucose / Q000494:pharmacology; D066292:Lipid Droplets / Q000737:chemistry* / Q000382:microbiology / Q000648:ultrastructure; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis* / Q000737:chemistry; D008856:Microscopy, Fluorescence; D019301:Oleic Acid / Q000494:pharmacology; D010802:Phylogeny; D040901:Proteomics; D012568:Schizosaccharomyces / Q000737:chemistry* / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D014280:Triglycerides / Q000032:analysis
chemical_list: D003470:Culture Media; D005227:Fatty Acids; D008055:Lipids; D029702:Schizosaccharomyces pombe Proteins; D014280:Triglycerides; D019301:Oleic Acid; D005947:Glucose
keywords: lipid droplets; lipid metabolism; proteomics; yeast