title: Determination of panduratin A in rat plasma by HPLC-MS/MS and its application to a pharmacokinetic study.
abstract: Although panduratin A, a chalcone derivative isolated from the rhizome of Kaempferia pandurata Roxb., represents a variety of pharmacological activities, no validated method for determination of panduratin A levels in biological samples is yet available. Thus, we developed a liquid chromatographic method using a tandem mass spectrometry for the determination of panduratin A in rat plasma. After simple protein precipitation with methanol including flufenamic acid (internal standard, IS), the analytes were chromatographed on a reversed-phased column with a mobile phase of distilled water and acetonitrile including 2% of formic acid (2:8, v/v). The ion transitions of the precursor to the product ion were principally deprotonated ions [M-H]-at m/z 405.2→165.9 for panduratin A and 280.1→236.0 for the IS. The accuracy and precision of the assay were in accordance with FDA regulations for the validation of bioanalytical methods. This analytical method was successfully applied to monitor the plasma concentrations of panduratin A following oral administration in rats.
authors.affiliation: College of Pharmacy, Chung-Ang University, Seoul 06974, South Korea.
authors.forename: Minsoo
authors.initials: M
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Chung-Ang University, Seoul 06974, South Korea.
authors.forename: Seungmok
authors.initials: S
authors.lastname: Choi
authors.affiliation: Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, 144 College Street, Toronto, Canada.
authors.forename: Keumhan
authors.initials: K
authors.lastname: Noh
authors.affiliation: Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, South Korea.
authors.forename: Changhee
authors.initials: C
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Chung-Ang University, Seoul 06974, South Korea.
authors.forename: Eunyoung
authors.initials: E
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, South Korea. Electronic address: jkhwang@yonsei.ac.kr.
authors.forename: Jae-Kwan
authors.initials: JK
authors.lastname: Hwang
authors.affiliation: College of Pharmacy, Chung-Ang University, Seoul 06974, South Korea. Electronic address: wkang@cau.ac.kr.
authors.forename: Wonku
authors.initials: W
authors.lastname: Kang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D047188:Chalcones / Q000097:blood* / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D010949:Plasma / Q000737:chemistry*; D051381:Rats; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D047188:Chalcones; C482884:panduratin A
keywords: HPLC–MS/MS; Panduratin A; Rat