title: Withania somnifera Root Extract Enhances Chemotherapy through 'Priming'.
abstract: Withania somnifera extracts are known for their anti-cancerous, anti-inflammatory and antioxidative properties. One of their mechanisms of actions is to modulate mitochondrial function through increasing oxidative stress. Recently 'priming' has been suggested as a potential mechanism for enhancing cancer cell death. In this study we demonstrate that 'priming', in HT-29 colon cells, with W. somnifera root extract increased the potency of the chemotherapeutic agent cisplatin. We have also showed the W. somnifera root extract enhanced mitochondrial dysfunction and that the underlying mechanism of 'priming' was selectively through increased ROS. Moreover, we showed that this effect was not seen in non-cancerous cells.
authors.affiliation: Department of Life Sciences, Faculty of Science and Technology, University of Westminster, London, United Kingdom.
authors.forename: Aine Brigette
authors.identifier: http://orcid.org/0000-0002-2271-7788
authors.initials: AB
authors.lastname: Henley
authors.affiliation: Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung, Taiwan.
authors.forename: Ling
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung, Taiwan.
authors.forename: Kun-Lin
authors.initials: KL
authors.lastname: Chuang
authors.affiliation: Department of Life Sciences, Faculty of Science and Technology, University of Westminster, London, United Kingdom.
authors.forename: Meliz
authors.initials: M
authors.lastname: Sahuri-Arisoylu
authors.affiliation: Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China.
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Wu
authors.affiliation: Department of Life Sciences, Faculty of Science and Technology, University of Westminster, London, United Kingdom.
authors.forename: S W Annie
authors.initials: SW
authors.lastname: Bligh
authors.affiliation: Department of Life Sciences, Faculty of Science and Technology, University of Westminster, London, United Kingdom.
authors.forename: Jimmy David
authors.initials: JD
authors.lastname: Bell
mesh_terms: D002945:Cisplatin / Q000008:administration & dosage; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000473:pathology; D019073:HT29 Cells; D006801:Humans; D008515:Medicine, Ayurvedic; D008928:Mitochondria / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D032341:Withania / Q000737:chemistry
chemical_list: D010936:Plant Extracts; D002945:Cisplatin