title: Transcriptome Analysis Reveals Comprehensive Insights into the Early Immune Response of Large Yellow Croaker (Larimichthys crocea) Induced by Trivalent Bacterial Vaccine.
abstract: Vaccination is an effective and safe strategy for combating bacterial diseases in fish, but the mechanisms underlying the early immune response after vaccination remain to be elucidated. In the present study, we used RNA-seq technology to perform transcriptome analysis of spleens from large yellow croaker (Larimichthys crocea) induced by inactivated trivalent bacterial vaccine (Vibrio parahaemolyticus, Vibrio alginolyticus and Aeromonas hydrophila). A total of 2,789 or 1,511 differentially expressed genes (DEGs) were obtained at 24 or 72 h after vaccination, including 1,132 or 842 remarkably up-regulated genes and 1,657 or 669 remarkably down-regulated genes, respectively. Gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichments revealed that numerous DEGs belong to immune-relevant genes, involved in many immune-relevant pathways. Most of the strongly up-regulated DEGs are innate defense molecules, such as antimicrobial peptides, complement components, lectins, and transferrins. Trivalent bacterial vaccine affected the expressions of many components associated with bacterial ligand-depending Toll-like receptor signaling pathways and inflammasome formation, indicating that multiple innate immune processes were activated at the early period of vaccination in large yellow croaker. Moreover, the expression levels of genes involved in antigen processing were also up-regulated by bacterial vaccine. However, the expression levels of several T cell receptors and related CD molecules and signal transducers were down-regulated, suggesting that the T cell receptor signaling pathway was rapidly suppressed after vaccination. These results provide the comprehensive insights into the early immune response of large yellow croaker to vaccination and valuable information for developing a highly immunogenic vaccine against bacterial infection in teleosts.
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo, China.|Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen, China.|Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo, China.|Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen, China.|Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, China.
authors.forename: Yinnan
authors.initials: Y
authors.lastname: Mu
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen, China.|Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Mu
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen, China.|Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, China.
authors.forename: Jingqun
authors.initials: J
authors.lastname: Ao
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen, China.|Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, China.|Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000235:genetics; D001428:Bacterial Vaccines / Q000276:immunology*; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D063990:Gene Ontology; D007109:Immunity / Q000235:genetics*; D058977:Molecular Sequence Annotation; D010473:Perciformes / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D017423:Sequence Analysis, RNA; D015398:Signal Transduction / Q000235:genetics; D059467:Transcriptome / Q000235:genetics; D014611:Vaccination
chemical_list: D001428:Bacterial Vaccines