title: Mung bean (Phaseolus radiatus L.) polyphenol extract attenuates aluminum-induced cardiotoxicity through an ROS-triggered Ca2+/JNK/NF-κB signaling pathway in rats.
abstract: Aluminum (Al) has been linked to the development of some cardiovascular diseases and mung bean is a functional food with the ability to detoxify. We aimed to evaluate the preventive effect and possible underlying mechanisms of the mung bean polyphenol extract (MPE) on Al-induced cardiotoxicity. Control, AlCl3(171.8 mg Al per kg body weight), MPE + AlCl3(Al-treatment plus 200 mg MPE per kg body weight), and a group of MPE per se were used. Al intake induced a significant increase of serum CK and LDH activity and the level of Na+, Ca2+, malondialdehyde and advanced oxidation protein products in the AlCl3-treated rats' heart tissue. Administration of MPE significantly improved the integrity and normal ion levels of heart tissue, and attenuated oxidative damage and the accumulation of Al in Al-treated rats. MPE significantly inhibited Al-induced increase of myocardial p-JNK, cytoplasmic NF-κB, cytochrome C, and caspase-9 protein expressions. Therefore, these results showed that MPE has a cardiac protective effect against Al-induced biotoxicity through ROS-JNK and NF-κB-mediated caspase pathways. Furthermore, the stability constant for the vitexin-Al complex was analyzed (log K = log K1+ log K2= 4.91 + 4.85 = 9.76). We found that MPE-mediated protection against Al-cardiotoxicity is connected both with MPE antioxidant and chelation properties.
authors.affiliation: Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, People's Republic of China. dcheng@tust.edu.cn.
authors.forename: Dai
authors.identifier: http://orcid.org/0000-0002-4956-948X
authors.initials: D
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, People's Republic of China. dcheng@tust.edu.cn.
authors.forename: Ruhua
authors.initials: R
authors.lastname: Wang
authors.affiliation: Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, People's Republic of China. dcheng@tust.edu.cn.
authors.forename: Chunling
authors.initials: C
authors.lastname: Wang
authors.affiliation: Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, People's Republic of China. dcheng@tust.edu.cn.
authors.forename: Lihua
authors.initials: L
authors.lastname: Hou
mesh_terms: D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000633:toxicity*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D066126:Cardiotoxicity / Q000188:drug therapy* / Q000209:etiology / Q000235:genetics / Q000378:metabolism; D053453:Caspase 9 / Q000235:genetics / Q000378:metabolism; D002712:Chlorides / Q000633:toxicity*; D006321:Heart / Q000187:drug effects; D006801:Humans; D048670:MAP Kinase Kinase 4 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D027805:Phaseolus / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage*; D059808:Polyphenols / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D017607:Aluminum Compounds; D002712:Chlorides; D016328:NF-kappa B; D010936:Plant Extracts; D059808:Polyphenols; D017382:Reactive Oxygen Species; D000077410:Aluminum Chloride; D008315:Malondialdehyde; D048670:MAP Kinase Kinase 4; D053453:Caspase 9; D002118:Calcium