title: Identification of the active protein in rice bran protein having an inhibitory activity of cholesterol micellar solubility.
abstract: In our previous study, rice bran protein (RBP) inhibited cholesterol micellar solubility in vitro and decreased serum cholesterol level in rats. In the present study, RBP was separated and purified by size-exclusion chromatography and reversed-phase chromatography. The active protein of RBP related to cholesterol micellar solubility was identified as lectin and non-specific lipid-transfer protein 1 using MALDI-TOF mass spectrometry analysis.
authors.affiliation: a Faculty of Applied Biological Sciences, Department of Applied Life Science , Gifu University , Gifu , Japan.
authors.forename: Jilite
authors.initials: J
authors.lastname: Wang
authors.affiliation: a Faculty of Applied Biological Sciences, Department of Applied Life Science , Gifu University , Gifu , Japan.
authors.forename: Masaya
authors.initials: M
authors.lastname: Shimada
authors.affiliation: a Faculty of Applied Biological Sciences, Department of Applied Life Science , Gifu University , Gifu , Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nagaoka
mesh_terms: D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification*; D002784:Cholesterol / Q000737:chemistry*; D002850:Chromatography, Gel; D056148:Chromatography, Reverse-Phase; D004043:Dietary Fiber / Q000032:analysis*; D008823:Micelles; D012275:Oryza / Q000737:chemistry*; D037121:Plant Lectins / Q000737:chemistry / Q000302:isolation & purification*; D016415:Sequence Alignment; D012995:Solubility
chemical_list: D002352:Carrier Proteins; D004043:Dietary Fiber; D008823:Micelles; D037121:Plant Lectins; C040599:rice lectin; C072087:sterol carrier proteins; D002784:Cholesterol
keywords: cholesterol; lectin; micelle; non-specific lipid-transfer protein 1; rice bran protein