title: Analysis of gene expression in granulosa cells post-maturation to evaluate oocyte culture systems in the domestic cat.
abstract: Maturation of oocytes is a prerequisite for successful embryo development. The fertilization competence of in vivo derived oocytes is significantly higher than that of oocytes matured in vitro. Commonly evaluated morphological criteria for oocyte maturation do not reflect the complexity and quality of maturation processes. Oocytes and granulosa cells are communicating closely in a bidirectional way during follicular growth and maturation. Assessing the mRNA expression of specific genes in granulosa cells could be a non-invasive way to evaluate the conditions of in vitro oocyte maturation. The objective of this study was to elucidate the influence of two different FSH additives on the in vitro maturation rate and gene expression of cumulus-oocytes complexes in domestic cat. Feline oocytes were matured in a medium, supplemented with LH and 0.02 IU/ml porcine FSH versus 0.02 IU or 1.06 IU/ml human FSH. Granulosa cells were separated from oocytes directly after 24 hr of maturation or after additional 12 hr of in vitro fertilization. Gene expression levels were analysed by quantitative PCR for aromatase, antimullerian hormone, follicle stimulating hormone receptor (FSHR), luteinizing hormone/choriogonadotropin receptor (LHCGR) and prostaglandin E synthase. Neither oocyte maturation rate nor gene expression levels differed after 24 or 36 hr in all three groups. However, variations were discovered in correlations of expression levels, for instance for FSHR and LHCG, indicating differences in the fine-tuning of in vitro maturation processes under varying FSH supplementations. We suppose that correlation between gene expressions of selected genes suggests a superior maturation quality of feline oocytes.
authors.affiliation: Leibniz-Institute for Zoo and Wildlife Research, Berlin, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Zahmel
authors.affiliation: Leibniz-Institute for Zoo and Wildlife Research, Berlin, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Mundt
authors.affiliation: Leibniz-Institute for Zoo and Wildlife Research, Berlin, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Jewgenow
authors.affiliation: Leibniz-Institute for Zoo and Wildlife Research, Berlin, Germany.
authors.forename: B C
authors.initials: BC
authors.lastname: Braun
mesh_terms: D000818:Animals; D001141:Aromatase / Q000235:genetics; D002415:Cats / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006107:Granulosa Cells / Q000378:metabolism*; D059471:In Vitro Oocyte Maturation Techniques; D007986:Luteinizing Hormone / Q000378:metabolism; D009865:Oocytes / Q000166:cytology*; D009866:Oogenesis; D011962:Receptors, FSH / Q000235:genetics / Q000378:metabolism*; D011974:Receptors, LH / Q000235:genetics / Q000378:metabolism*
chemical_list: D011962:Receptors, FSH; D011974:Receptors, LH; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D001141:Aromatase