title: Towards a FISH-based karyotype ofRosaL. (Rosaceae).
abstract: The genusRosaLinnaeus, 1753 has important economic value in ornamental sector and many breeding activities are going on supported by molecular studies. However, the cytogenetic studies of rose species are scarce and mainly focused on chromosome counting and chromosome morphology-based karyotyping. Due to the small size of the chromosomes and a high frequency of polyploidy in the genus, karyotyping is very challenging for rose species and requires FISH-based cytogenetic markers to be applied. Therefore, in this work the aim is to establish a FISH-based karyotype forRosa wichurana(Crépin, 1888), a rose species with several benefits for advanced molecular cytogenetic studies of genusRosa(Kirov et al. 2015a). It is shown that FISH signals from 5S, 45S and anArabidopsis-type telomeric repeat are distributed on five (1, 2, 4, 5 and 7) of seven chromosome pairs. In addition, it is demonstrated that the interstitial telomeric repeat sequences (ITR) are located in the centromeric regions of four chromosome pairs. Using low hybridization stringency for ITR visualization, we showed that the number of ITR signals increases four times (1-4 signals). This study is the first to propose a FISH-basedRosa wichuranakaryotype for the reliable identification of chromosomes. The possible origin ofRosa wichuranaITR loci is discussed.
authors.affiliation: Center of Molecular Biotechnology, Russian State Agrarian University - Moscow Timiryazev Agricultural Academy, Timiryazevskay str. 49, 127550, Moscow, Russia; Department of Genetics and Biotechnology, Russian State Agrarian University - Moscow Timiryazev Agricultural Academy, Timiryazevskay str. 3, 127550, Moscow, Russia; Institute for Agricultural and Fisheries Research (ILVO), Plant Sciences Unit, Applied Genetics and Breeding, Caritasstraat 39, 9090, Melle, Belgium.
authors.forename: Ilya V
authors.initials: IV
authors.lastname: Kirov
authors.affiliation: Institute for Agricultural and Fisheries Research (ILVO), Plant Sciences Unit, Applied Genetics and Breeding, Caritasstraat 39, 9090, Melle, Belgium.
authors.forename: Katrijn
authors.initials: K
authors.lastname: Van Laere
authors.affiliation: Center of Medical Genetics, Faculty of Medicine and Health Sciences, Ghent University, De Pintelaan 185, 9000, Ghent, Belgium.
authors.forename: Nadine
authors.initials: N
authors.lastname: Van Roy
authors.affiliation: Center of Molecular Biotechnology, Russian State Agrarian University - Moscow Timiryazev Agricultural Academy, Timiryazevskay str. 49, 127550, Moscow, Russia; Department of Genetics and Biotechnology, Russian State Agrarian University - Moscow Timiryazev Agricultural Academy, Timiryazevskay str. 3, 127550, Moscow, Russia.
authors.forename: Ludmila I
authors.initials: LI
authors.lastname: Khrustaleva
keywords: 45S rDNA; 5S rDNA; Cytogenetic markers; Rosa wichurana; fluorescence in situ hybridization; interstitial telomeric repeat (ITR)