title: In vivo gene expression profiling for chemosensitivity to docetaxel-cisplatin-5-FU (TPF) triplet regimen in laryngeal squamous cell carcinoma and the effect of TPF treatment on related gene expression in vitro.
abstract: CONCLUSIONS
  These results provided a battery of genes relating to TPF chemotherapeutic sensitivity and might act as molecular targets in laryngeal squamous cell carcinoma (LSCC) treatment. Moreover, these candidate biomarkers could contribute to LSCC individualized treatment.
  
  
  OBJECTIVE
  To screen out a set of candidate genes which could help to determine whether patients with LSCC could benefit from TPF induction chemotherapy.
  
  
  METHODS
  Gene-expression profiles in seven TPF-sensitive patients were compared to four resistant controls by microarray analysis. Subsequently, expression levels of potential biomarkers in chemosensitive cell line UMSCC5 after TPF treatment were observed by qRT-PCR.
  
  
  RESULTS
  Through microarray analysis, 1546 differently expressed genes were identified, of which 769 were up-regulated in TPF chemotherapy-responsive tissues, whereas 777 were down-regulated. Gene ontology (GO) analysis suggested these genes participating in physiological processes including cell differentiation, metabolism, signal transduction, and cellular component organization. Additionally, Kyoto Encyclopedia of Genes and Genomes (KEGG) database revealed that Wnt and p53 signaling pathways occupied important roles in TPF chemotherapeutic sensitivity. Moreover, in vitro cell culture experiments revealed the expression alternations of Mapk10, Jun, Vegfb, Pik3r5, Pld1, Tek, Itga6 exposed to TPF treatment by qRT-PCR, whilst providing an insight into the mechanism underlying TPF chemotherapeutic response in LSCC.
authors.affiliation: a Department of Otorhinolaryngology Head and Neck Surgery , Beijing Tongren Hospital, Capital Medical University , Beijing , PR China.
authors.forename: Meng
authors.initials: M
authors.lastname: Lian
authors.affiliation: a Department of Otorhinolaryngology Head and Neck Surgery , Beijing Tongren Hospital, Capital Medical University , Beijing , PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Shi
authors.affiliation: a Department of Otorhinolaryngology Head and Neck Surgery , Beijing Tongren Hospital, Capital Medical University , Beijing , PR China.
authors.forename: Jugao
authors.initials: J
authors.lastname: Fang
authors.affiliation: a Department of Otorhinolaryngology Head and Neck Surgery , Beijing Tongren Hospital, Capital Medical University , Beijing , PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Feng
authors.affiliation: a Department of Otorhinolaryngology Head and Neck Surgery , Beijing Tongren Hospital, Capital Medical University , Beijing , PR China.
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Ma
authors.affiliation: a Department of Otorhinolaryngology Head and Neck Surgery , Beijing Tongren Hospital, Capital Medical University , Beijing , PR China.
authors.forename: Haizhou
authors.initials: H
authors.lastname: Wang
authors.affiliation: b Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of Education , Beijing Institute of Otorhinolaryngology , Beijing , PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: b Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of Education , Beijing Institute of Otorhinolaryngology , Beijing , PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: c Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology , Beijing , PR China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Ma
authors.affiliation: c Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology , Beijing , PR China.
authors.forename: Honggang
authors.initials: H
authors.lastname: Liu
mesh_terms: D000368:Aged; D000970:Antineoplastic Agents; D000971:Antineoplastic Combined Chemotherapy Protocols; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000378:metabolism*; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007822:Laryngeal Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D060449:Wnt Signaling Pathway
chemical_list: D000970:Antineoplastic Agents
keywords: LSCC; TPF; chemotherapeutic sensitivity in vivo; gene expression alteration in vitro; microarray