title: Lipopolysaccharide promotes the development of murine endometriosis-like lesions via the nuclear factor-kappa B pathway.
abstract: Is lipopolysaccharide (LPS) involved in the development of endometriosis?
  
  
  
  BALB/c mice (n=69) were used for the murine endometriosis model. Mice with surgically induced endometriosis were injected with LPS intraperitoneally. After 4 weeks of LPS injections with or without the nuclear factor-kappa B (NF-κB) inhibitor, the extent of endometriosis-like lesions was evaluated. Expression of inflammatory factors in the implants was evaluated using real-time RT-PCR. Cell proliferation, angiogenic activity, inflammation, and NF-κB phosphorylation were assessed by immunohistochemical staining.
  
  
  
  Lipopolysaccharide increased total number, size, and mRNA expression of Ptgs-2, Vegf, Ccl-2, and Il-6 in endometriosis-like lesions. LPS also increased the percentage of Ki67-positive cells and enhanced the intensity and rate of positive cells of CD3, F4/80, and PECAM. Intense expression of phospho-NF-κB p65 after LPS administration was observed. Treatment with the NF-kB inhibitor negated these LPS-induced effects.
  
  
  
  LPS-induced pelvic inflammation status enhanced the development of murine endometriosis-like lesions via NF-κB pathway.
authors.affiliation: Department of Obstetrics and Gynecology, Tottori University Faculty of Medicine, Yonago, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Azuma
authors.affiliation: Department of Obstetrics and Gynecology, Tottori University Faculty of Medicine, Yonago, Japan.
authors.forename: Fuminori
authors.initials: F
authors.lastname: Taniguchi
authors.affiliation: Division of Laboratory Animal Science, Research Center for Bioscience and Technology, Tottori University Faculty of Medicine, Yonago, Japan.
authors.forename: Kazuomi
authors.initials: K
authors.lastname: Nakamura
authors.affiliation: Department of Obstetrics and Gynecology, Tottori University Faculty of Medicine, Yonago, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Nagira
authors.affiliation: Department of Obstetrics and Gynecology, Tottori University Faculty of Medicine, Yonago, Japan.
authors.forename: Yin Mon
authors.initials: YM
authors.lastname: Khine
authors.affiliation: Department of Obstetrics and Gynecology, Tottori University Faculty of Medicine, Yonago, Japan.
authors.forename: Tomoiki
authors.initials: T
authors.lastname: Kiyama
authors.affiliation: Department of Obstetrics and Gynecology, Tottori University Faculty of Medicine, Yonago, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Uegaki
authors.affiliation: Department of Obstetrics and Gynecology, Tottori University Faculty of Medicine, Yonago, Japan.
authors.forename: Masao
authors.initials: M
authors.lastname: Izawa
authors.affiliation: Department of Obstetrics and Gynecology, Tottori University Faculty of Medicine, Yonago, Japan.
authors.forename: Tasuku
authors.initials: T
authors.lastname: Harada
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004715:Endometriosis / Q000378:metabolism / Q000473:pathology*; D005260:Female; D007150:Immunohistochemistry; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D008070:Lipopolysaccharides / Q000633:toxicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*
chemical_list: D008070:Lipopolysaccharides; D016328:NF-kappa B
keywords: 
  LPS
  ; NF-κB; inflammation; murine endometriosis-like lesions