title: Cell-Free DNA Integrity Analysis in Urine Samples.
abstract: Although the presence of circulating cell-free DNA in plasma or serum has been widely shown to be a suitable source of biomarkers for many types of cancer, few studies have focused on the potential use of urine cell-free (UCF) DNA. Starting from the hypotheses that normal apoptotic cells produce highly fragmented DNA and that cancer cells release longer DNA, the potential role of UCF DNA integrity was evaluated as an early diagnostic marker capable of distinguishing between patients with prostate or bladder cancer and healthy individuals. A UCF DNA integrity analysis is proposed on the basis of four quantitative real-time PCRs of four sequences longer than 250 bp: c-MYC, BCAS1, HER2, and AR. Sequences that frequently have an increased DNA copy number in bladder and prostate cancers were chosen for the analysis, but the method is flexible, and these genes could be substituted with other genes of interest. The potential utility of UCF DNA as a source of biomarkers has already been demonstrated for urologic malignancies, thus paving the way for further studies on UCF DNA characterization. The UCF DNA integrity test has the advantage of being non-invasive, rapid, and easy to perform, with only a few milliliters of urine needed to carry out the analysis.
authors.affiliation: Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS; valentina.casadio@irst.emr.it.
authors.forename: Valentina
authors.initials: V
authors.lastname: Casadio
authors.affiliation: Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS.
authors.forename: Samanta
authors.initials: S
authors.lastname: Salvi
authors.affiliation: Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS.
authors.forename: Filippo
authors.initials: F
authors.lastname: Martignano
authors.affiliation: Department of Urology, Morgagni-Pierantoni Hospital.
authors.forename: Roberta
authors.initials: R
authors.lastname: Gunelli
authors.affiliation: Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS.
authors.forename: Sara
authors.initials: S
authors.lastname: Ravaioli
authors.affiliation: Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS.
authors.forename: Daniele
authors.initials: D
authors.lastname: Calistri
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000652:urine*; D000073888:Cell-Free Nucleic Acids / Q000652:urine*; D006801:Humans; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics; D013053:Spectrophotometry; D016482:Urinalysis; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D000073888:Cell-Free Nucleic Acids; D009363:Neoplasm Proteins; D016271:Proto-Oncogene Proteins c-myc; D018719:Erb-b2 Receptor Tyrosine Kinases; C113699:BCAS1 protein, human