title: Comparative Effects of Non-Thermal Atmospheric Pressure Plasma on Migration and Invasion in Oral Squamous Cell Cancer, by Gas Type.
abstract: OBJECTIVE
  The fourth state of matter, plasma is known as an ionized gas with electrons, radicals and ions. The use of non-thermal plasma (NTP) in cancer research became possible because of the progresses in plasma medicine. Previous studies on the potential NTP-mediated cancer therapy have mainly concentrated on cancer cell apoptosis. In the present study, we compared the inhibitory effect of NTP on cell migration and invasion in the oral squamous cancer cell lines.
  
  
  METHODS
  We used oral squamous cancer cell lines (SCC1483, MSKQLL1) and different gases (N₂, He, and Ar). To investigate the mechanism of plasma treatment, using different gases (N₂, He, and Ar) which induces anti-migration and anti-invasion properties, we performed wound healing assay, invasion assay and gelatin zymography.
  
  
  RESULTS
  The results showed that NTP inhibits cancer cell migration and invasion of oral squamous cancer cell. In addition, focal adhesion kinase expression and matrix metalloproteinase-2/9 activity were also inhibited.
  
  
  CONCLUSIONS
  The suppression of cancer cell invasion by NTP varied depending on the type of gas. Comparison of the three gases revealed that N₂ NTP inhibited cell migration and invasion most potently via decreased expression of focal adhesion kinase and matrix metalloproteinase activity.
authors.affiliation: Department of Otolaryngology, Ajou University School of Medicine, Suwon, Korea.
authors.forename: Sung Un
authors.initials: SU
authors.lastname: Kang
authors.affiliation: Department of Otolaryngology, Ajou University School of Medicine, Suwon, Korea.
authors.forename: Seong Jin
authors.initials: SJ
authors.lastname: Seo
authors.affiliation: Department of Otorhinolaryngology, Konyang University College of Medicine, Daejeon, Korea.
authors.forename: Yeon Soo
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Department of Otolaryngology, Ajou University School of Medicine, Suwon, Korea.
authors.forename: Yoo Seob
authors.initials: YS
authors.lastname: Shin
authors.affiliation: Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Yoon Woo
authors.initials: YW
authors.lastname: Koh
authors.affiliation: Department of Electrical and Computer Engineering, Ajou University, Suwon, Korea.
authors.forename: Chang Min
authors.initials: CM
authors.lastname: Lee
authors.affiliation: Department of Electrical and Computer Engineering, Ajou University, Suwon, Korea.
authors.forename: Sang Sik
authors.initials: SS
authors.lastname: Yang
authors.affiliation: Department of Life Science, Ajou University, Suwon, Korea.
authors.forename: Jong Soo
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of Life Science, Ajou University, Suwon, Korea.
authors.forename: Eunpyo
authors.initials: E
authors.lastname: Moon
authors.affiliation: Ajou University, Suwon, Korea.
authors.forename: Hami
authors.initials: H
authors.lastname: Kang
authors.affiliation: ICD Co., Ltd., Anseong, Korea.
authors.forename: Jeong Beom
authors.initials: JB
authors.lastname: Ryeo
authors.affiliation: ICD Co., Ltd., Anseong, Korea.
authors.forename: Yuijina
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Department of Otolaryngology, Ajou University School of Medicine, Suwon, Korea. ostium@ajou.ac.kr.
authors.forename: Chul Ho
authors.initials: CH
authors.lastname: Kim
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D051416:Focal Adhesion Protein-Tyrosine Kinases; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D009062:Mouth Neoplasms / Q000378:metabolism / Q000628:therapy*; D009361:Neoplasm Invasiveness; D058626:Plasma Gases / Q000494:pharmacology*; D014945:Wound Healing
chemical_list: D058626:Plasma Gases; D051416:Focal Adhesion Protein-Tyrosine Kinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9
keywords: Non thermal atmospheric pressure plasma; focal adhesion kinase; matrix metallopeptidase-2/9; oral squamous cancer cell; paxillin