title: Release of Type 2 Cytokines by Epithelial Cells of Nasal Polyps.
abstract: Background. T2 inflammation of chronic rhinosinusitis with nasal polyps (CRSwNP) may be influenced by epithelial cytokines release (TSLP, IL-25, and IL-33). We investigated the release of TSLP, IL-25, and IL-33 by epithelial CRSwNP cells compared to epithelial sinus mucosa cells of patients with chronic rhinosinusitis without nasal polyps (CRSsNP).Methods. IL-25, IL-33, and TSLP were measured by ELISA in the supernatant of cell cultures derived by CRSwNP (9 patients, 6 atopic) and CRSsNP (7 patients, 2 atopic) in baseline condition and following stimulation withDermatophagoides pteronyssinus(DP),Aspergillus fumigatus(AF), and poly(I:C).Results. CRSwNP epithelial cells released increased levels of IL-25 (from 0.12 ± 0.06 pg/ml to 0.27 ± 0.1 pg/ml,p< 0.01) and TSLP (from 0.77 ± 0.5 pg/ml to 2.53 ± 1.17 pg/ml,p< 0.001) following poly(I:C) stimulation, while CRSsNP epithelial cells released increased levels of IL-25 and IL-33 following AF and DP stimulation, respectively (IL-25: from 0.18 ± 0.07 pg/ml to 0.51 ± 0.1 pg/ml,p< 0.001; IL-33: from 2.57 ± 1.3 pg/ml to 5.7 ± 3.1 pg/ml,p< 0.001).Conclusions. CRSwNP epithelial cells release TSLP and IL-25 when stimulated by poly(I:C) but not by DP or AF, suggesting that viral infection may contribute to maintain and amplify the T2 immune response seen in CRSwNP.
authors.affiliation: Department of Medical Sciences, University of Torino, Turin, Italy.
authors.forename: Monica
authors.identifier: 0000-0003-4925-037X
authors.initials: M
authors.lastname: Boita
authors.affiliation: Department of Medical Sciences, University of Torino, Turin, Italy.
authors.forename: Caterina
authors.initials: C
authors.lastname: Bucca
authors.affiliation: 1 ENT Division, Department of Surgical Sciences, University of Torino, Turin, Italy.
authors.forename: Giuseppe
authors.identifier: 0000-0002-1825-7635
authors.initials: G
authors.lastname: Riva
authors.affiliation: Respiratory Medicine and Allergology, Department of Clinical and Experimental Medicine, University of Catania, Catania, Italy.
authors.forename: Enrico
authors.identifier: 0000-0002-0492-5663
authors.initials: E
authors.lastname: Heffler
authors.affiliation: Department of Medical Sciences, University of Torino, Turin, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Rolla
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology; D001232:Aspergillus fumigatus / Q000276:immunology; D002478:Cells, Cultured; D002908:Chronic Disease; D003470:Culture Media; D016207:Cytokines / Q000276:immunology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000276:immunology; D005260:Female; D006801:Humans; D020381:Interleukin-17 / Q000276:immunology / Q000378:metabolism*; D000067596:Interleukin-33 / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000276:immunology*; D009298:Nasal Polyps / Q000276:immunology*; D011070:Poly I-C / Q000276:immunology; D011107:Polylysine / Q000276:immunology; D012220:Rhinitis / Q000276:immunology*; D012852:Sinusitis / Q000276:immunology*; D055815:Young Adult; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D039741:Antigens, Dermatophagoides; D003470:Culture Media; D016207:Cytokines; C486701:IL25 protein, human; C506536:IL33 protein, human; D020381:Interleukin-17; D000067596:Interleukin-33; D011107:Polylysine; C040125:poly ICL; D011070:Poly I-C; D000094632:Thymic Stromal Lymphopoietin