title: Mussel-Inspired Multifunctional Hydrogel Coating for Prevention of Infections and Enhanced Osteogenesis.
abstract: Prevention of postsurgery infection and promotion of biointegration are the key factors to achieve long-term success in orthopedic implants. Localized delivery of antibiotics and bioactive molecules by the implant surface serves as a promising approach toward these goals. However, previously reported methods for surface functionalization of the titanium alloy implants to load bioactive ingredients suffer from time-consuming complex processes and lack of long-term stability. Here, we present the design and characterization of an adhesive, osteoconductive, and antimicrobial hydrogel coating for Ti implants. To form this multifunctional hydrogel, a photo-cross-linkable gelatin-based hydrogel was modified with catechol motifs to enhance adhesion to Ti surfaces and thus promote coating stability. To induce antimicrobial and osteoconductive properties, a short cationic antimicrobial peptide (AMP) and synthetic silicate nanoparticles (SNs) were introduced into the hydrogel formulation. The controlled release of AMP loaded in the hydrogel demonstrated excellent antimicrobial activity to prevent biofilm formation. Moreover, the addition of SNs to the hydrogel formulation enhanced osteogenesis when cultured with human mesenchymal stem cells, suggesting the potential to promote new bone formation in the surrounding tissues. Considering the unique features of our implant hydrogel coating, including high adhesion, antimicrobial capability, and the ability to induce osteogenesis, it is believed that our design provides a useful alternative method for bone implant surface modification and functionalization.
authors.affiliation: Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School , Cambridge, Massachusetts 02139, United States.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.|Othopeadic Department, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan 430030, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School , Cambridge, Massachusetts 02139, United States.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.
authors.forename: Kan
authors.initials: K
authors.lastname: Yue
authors.affiliation: Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School , Cambridge, Massachusetts 02139, United States.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Kazemzadeh-Narbat
authors.affiliation: Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School , Cambridge, Massachusetts 02139, United States.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.|College of Textiles, Donghua University , Shanghai, 201620, China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School , Cambridge, Massachusetts 02139, United States.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.
authors.forename: Akbar
authors.initials: A
authors.lastname: Khalilpour
authors.affiliation: Department of Orthopaedics, School of Medicine, West Virginia University , Morgantown, West Virginia 26506, United States.
authors.forename: Bingyun
authors.initials: B
authors.lastname: Li
authors.affiliation: Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School , Cambridge, Massachusetts 02139, United States.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.
authors.forename: Yu Shrike
authors.identifier: http://orcid.org/0000-0001-8642-133X
authors.initials: YS
authors.lastname: Zhang
authors.affiliation: Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School , Cambridge, Massachusetts 02139, United States.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.|Department of Chemical Engineering, Northeastern University , Boston, Massachusetts 02115-5000, United States.
authors.forename: Nasim
authors.initials: N
authors.lastname: Annabi
authors.affiliation: Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School , Cambridge, Massachusetts 02139, United States.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.|Department of Bioindustrial Technologies, College of Animal Bioscience and Technology, Konkuk University , Seoul, 143-701, the Republic of Korea.|Department of Physics, King Abdulaziz University , Jeddah 21569, Saudi Arabia.
authors.forename: Ali
authors.identifier: http://orcid.org/0000-0002-2692-1524
authors.initials: A
authors.lastname: Khademhosseini
mesh_terms: D000818:Animals; D049872:Bivalvia; D020099:Coated Materials, Biocompatible; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D059630:Mesenchymal Stem Cells; D010012:Osteogenesis; D014025:Titanium
chemical_list: D020099:Coated Materials, Biocompatible; D020100:Hydrogels; D014025:Titanium
keywords: adhesive hydrogels; antimicrobial; osteogenesis; silicate nanoparticles; titanium implant