title: Comparison of Periodontal Ligament Stem Cells Isolated from the Periodontium of Healthy Teeth and Periodontitis-Affected Teeth.
abstract: OBJECTIVE
  Stem cell (SC) therapy is a promising technique for tissue regeneration. This study aimed to compare the viability and proliferation ability of periodontal ligament stem cells (PDLSCs) isolated from the periodontium of healthy and periodontitis-affected teeth to obtain an autologous, easily accessible source of SCs for tissue regeneration in periodontitis patients.
  
  
  METHODS
  The PDLSCs were isolated from the roots of clinically healthy premolars extracted for orthodontic purposes and periodontally involved teeth with hopeless prognosis (with and without phase I periodontal treatment). Cells were cultured and viability and proliferation ability of third passage cells in each group were evaluated using the methyl thiazol tetrazolium assay. The results were statistically analyzed using t-test.
  
  
  RESULTS
  No SCs could be obtained from periodontitis-affected teeth without phase I periodontal treatment. The viability of cells was 0.86±0.13 OD/540 in healthy group and 0.4±0.25 OD/540 in periodontitis-affected group (P=0.035). The proliferation ability (population doubling time) of cells obtained from healthy teeth was 4.22±1.23 hours. This value was 2.3±0.35 hours for those obtained from periodontitis-affected teeth (P=0.02).
  
  
  CONCLUSIONS
  Viability and proliferation ability of cells isolated from the periodontium of healthy teeth were significantly greater than those of cells isolated from the periodontitis-affected teeth.
authors.affiliation: Assistant Professor, Department of Periodontics, Faculty of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Sara
authors.initials: S
authors.lastname: Soheilifar
authors.affiliation: Professor, Endometrium and Endometriosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Iraj
authors.initials: I
authors.lastname: Amiri
authors.affiliation: Assistant Professor, Department of Periodontics, Faculty of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Bidgoli
authors.affiliation: Assistant Professor, Department of Periodontics, Faculty of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Morad
authors.initials: M
authors.lastname: Hedayatipanah
keywords: Periodontitis; Regeneration; Stem Cells; Tooth