title: Effect of troxerutin on 2-aminoanthracene and DNA interaction and its anti-mutagenic property.
abstract: One of the pivotal mechanisms projected for bioflavonoids in cancer chemoprevention is through their intervention against mutagen-DNA interaction. Recent literatures emphasize the role of troxerutin (TXER) as an emerging anticancer agent. However, there are no reports on its intervention in any carcinogen-DNA interaction. The present study investigates the possibility of TXER, in prevention of 2-aminoanthracene (2-AA) contact with DNA. Steady state and time resolved fluorescence spectroscopy results, highlight the direct contact of 2-AA with DNA, while presence of TXER prevented this interaction. Gel-electrophoresis study clearly revealed that, TXER inhibits 2-AA+UVA radiation induced DNA damage. Fluorescence microscopic studies elucidated that, TXER treatment obstructs the 2-AA interaction with cellular DNA, while molecular docking showed the energetically favourable structure of TXER/2-AA/TXER complex. Further anti-mutagenicity experiment revealed that, TXER prevents the mutation induced colony formation in mutant strain of S. typhymurium. Our in vitro and ex vivo experimental findings provide imperative evidence about the protective role of TXER against environmental carcinogens through the inhibition of carcinogen-DNA interaction, implicating its potential for therapeutic applications in cancer.
authors.affiliation: Department of Biochemistry and Molecular Biology, Pondicherry University, Puducherry 605 014, India.
authors.forename: A
authors.initials: A
authors.lastname: Subastri
authors.affiliation: Centre for Bioinformatics, Pondicherry University, Puducherry 605 014, India.
authors.forename: K
authors.initials: K
authors.lastname: Harikrishna
authors.affiliation: Centre for Bioinformatics, Pondicherry University, Puducherry 605 014, India.
authors.forename: M
authors.initials: M
authors.lastname: Sureshkumar
authors.affiliation: Department of Food Science and Nutrition, College of Food and Agricultural Science, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia.
authors.forename: Ghedeir M
authors.initials: GM
authors.lastname: Alshammari
authors.affiliation: Department of Food Science and Nutrition, College of Food and Agricultural Science, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia.
authors.forename: B
authors.initials: B
authors.lastname: Aristatile
authors.affiliation: Department of Biochemistry and Molecular Biology, Pondicherry University, Puducherry 605 014, India. Electronic address: tchinnasamy@hotmail.com.
authors.forename: C
authors.initials: C
authors.lastname: Thirunavukkarasu
mesh_terms: D016475:3T3 Cells; D000818:Animals; D000873:Anthracenes / Q000633:toxicity*; D016587:Antimutagenic Agents / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage / Q000187:drug effects; D005838:Genotype; D006896:Hydroxyethylrutoside / Q000031:analogs & derivatives* / Q000494:pharmacology; D051379:Mice; D008958:Models, Molecular; D062105:Molecular Docking Simulation; D009153:Mutagens / Q000633:toxicity*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D029909:Sophora / Q000737:chemistry; D014466:Ultraviolet Rays
chemical_list: D000873:Anthracenes; D016587:Antimutagenic Agents; D006896:Hydroxyethylrutoside; D009153:Mutagens; C005865:troxerutin; C006593:2-anthramine; D004247:DNA
keywords: 2-aminoanthracene; Carcinogen; DNA-interaction; Flavonoid; Troxerutin