title: IL-7 Induces an Epitope Masking ofγc Protein in IL-7 Receptor Signaling Complex.
abstract: IL-7 signaling via IL-7Rαand commonγ-chain (γc) is necessary for the development and homeostasis of T cells. Although the delicate mechanism in which IL-7Rαdownregulation allows the homeostasis of T cell with limited IL-7 has been well known, the exact mechanism behind the interaction between IL-7Rαandγc in the absence or presence of IL-7 remains unclear. Additionally, we are still uncertain as to how only IL-7Rαis separately downregulated by the binding of IL-7 from the IL-7Rα/γc complex. We demonstrate here that 4G3, TUGm2, and 3E12 epitope masking ofγc protein are induced in the presence of IL-7, indicating that the epitope alteration is induced by IL-7 binding to the preassembled receptor core. Moreover, the epitope masking ofγc protein is inversely correlated with the expression of IL-7Rαupon IL-7 binding, implying that the structural alteration ofγc might be involved in the regulation of IL-7Rαexpression. The conformational change inγc upon IL-7 binding may contribute not only to forming the functional IL-7 signaling complex but also to optimally regulating the expression of IL-7Rα.
authors.affiliation: Department of Anatomy and Cell Biology, Pusan National University School of Medicine, Yangsan, Republic of Korea; Department of Orthopedic Surgery, Medical Research Institute, Pusan National University School of Medicine, Busan, Republic of Korea.
authors.forename: Tae Sik
authors.initials: TS
authors.lastname: Goh
authors.affiliation: Department of Anatomy and Cell Biology, Pusan National University School of Medicine, Yangsan, Republic of Korea.
authors.forename: Yuna
authors.initials: Y
authors.lastname: Jo
authors.affiliation: Department of Anatomy and Cell Biology, Pusan National University School of Medicine, Yangsan, Republic of Korea.
authors.forename: Byunghyuk
authors.initials: B
authors.lastname: Lee
authors.affiliation: Department of Anatomy and Cell Biology, Pusan National University School of Medicine, Yangsan, Republic of Korea.
authors.forename: Geona
authors.initials: G
authors.lastname: Kim
authors.affiliation: Department of Anatomy and Cell Biology, Pusan National University School of Medicine, Yangsan, Republic of Korea.
authors.forename: Hyunju
authors.initials: H
authors.lastname: Hwang
authors.affiliation: Department of Anatomy and Cell Biology, Pusan National University School of Medicine, Yangsan, Republic of Korea.
authors.forename: Eunhee
authors.initials: E
authors.lastname: Ko
authors.affiliation: Department of Anatomy and Cell Biology, Pusan National University School of Medicine, Yangsan, Republic of Korea.
authors.forename: Seung Wan
authors.initials: SW
authors.lastname: Kang
authors.affiliation: Department of Anatomy and Cell Biology, Pusan National University School of Medicine, Yangsan, Republic of Korea.
authors.forename: Sae-Ock
authors.initials: SO
authors.lastname: Oh
authors.affiliation: Department of Anatomy and Cell Biology, Pusan National University School of Medicine, Yangsan, Republic of Korea.
authors.forename: Sun-Yong
authors.initials: SY
authors.lastname: Baek
authors.affiliation: Department of Anatomy and Cell Biology, Pusan National University School of Medicine, Yangsan, Republic of Korea.
authors.forename: Sik
authors.initials: S
authors.lastname: Yoon
authors.affiliation: Department of Orthopedic Surgery, Medical Research Institute, Pusan National University School of Medicine, Busan, Republic of Korea.
authors.forename: Jung Sub
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of Anatomy and Cell Biology, Pusan National University School of Medicine, Yangsan, Republic of Korea.
authors.forename: Changwan
authors.identifier: 0000-0003-2567-2973
authors.initials: C
authors.lastname: Hong
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D016207:Cytokines / Q000378:metabolism; D000939:Epitopes / Q000737:chemistry*; D006801:Humans; D053631:Interleukin Receptor Common gamma Subunit / Q000737:chemistry*; D015851:Interleukin-7 / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D008810:Mice, Inbred C57BL; D010766:Phosphorylation; D011485:Protein Binding; D000072417:Protein Domains; D020395:Receptors, Interleukin-7 / Q000737:chemistry*; D015398:Signal Transduction
chemical_list: D000911:Antibodies, Monoclonal; D016207:Cytokines; D000939:Epitopes; C508605:IL7 protein, human; D053631:Interleukin Receptor Common gamma Subunit; D015851:Interleukin-7; D020395:Receptors, Interleukin-7; C415350:interleukin-7 receptor, alpha chain