title: TSA and BIX-01294 Induced Normal DNA and Histone Methylation and Increased Protein Expression in Porcine Somatic Cell Nuclear Transfer Embryos.
abstract: The poor efficiency of animal cloning is mainly attributed to the defects in epigenetic reprogramming of donor cells' chromatins during early embryonic development. Previous studies indicated that inhibition of histone deacetylases or methyltransferase, such as G9A, using Trichostatin A (TSA) or BIX-01294 significantly enhanced the developmental efficiency of porcine somatic cell nuclear transfer (SCNT) embryos. However, potential mechanisms underlying the improved early developmental competence of SCNT embryos exposed to TSA and BIX-01294 are largely unclear. Here we found that 50 nM TSA or 1.0 μM BIX-01294 treatment alone for 24 h significantly elevated the blastocyst rate (P < 0.05), while further improvement was not observed under combined treatment condition. Furthermore, co-treatment or TSA treatment alone significantly reduced H3K9me2 level at the 4-cell stage, which is comparable with that in in vivo and in vitro fertilized counterparts. However, only co-treatment significantly decreased the levels of 5mC and H3K9me2 in trophectoderm lineage and subsequently increased the expression of OCT4 and CDX2 associated with ICM and TE lineage differentiation. Altogether, these results demonstrate that co-treatment of TSA and BIX-01294 enhances the early developmental competence of porcine SCNT embryos via improvements in epigenetic status and protein expression.
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
authors.forename: Zubing
authors.initials: Z
authors.lastname: Cao
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
authors.forename: Renyun
authors.initials: R
authors.lastname: Hong
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Ding
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Zuo
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Ding
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
authors.forename: Yunsheng
authors.initials: Y
authors.lastname: Li
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Huang
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
authors.forename: Yunhai
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001381:Azepines / Q000494:pharmacology*; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects; D019070:Cell Lineage; D004247:DNA / Q000378:metabolism*; D019175:DNA Methylation; D004622:Embryo, Mammalian / Q000187:drug effects* / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D006877:Hydroxamic Acids / Q000494:pharmacology*; D053652:Nuclear Transfer Techniques; D011799:Quinazolines / Q000494:pharmacology*; D013552:Swine
chemical_list: D001381:Azepines; C518299:BIX 01294; D006657:Histones; D006877:Hydroxamic Acids; D011799:Quinazolines; C012589:trichostatin A; D004247:DNA