title: Adipose-Derived Stem Cells Cocultured with Chondrocytes Promote the Proliferation of Chondrocytes.
abstract: Articular cartilage injury and defect caused by trauma and chronic osteoarthritis vascularity are very common, while the repair of injured cartilage remains a great challenge due to its limited healing capacity. Stem cell-based tissue engineering provides a promising treatment option for injured articular cartilage because of the cells potential for multiple differentiations. However, its application has been largely limited by stem cell type, number, source, proliferation, and differentiation. We hypothesized that (1) adipose-derived stem cells are ideal seed cells for articular cartilage repair because of their accessibility and abundance and (2) the microenvironment of articular cartilage could induce adipose-derived stem cells (ADSCs) to differentiate into chondrocytes. In order to test our hypotheses, we isolated stem cells from rabbit adipose tissues and cocultured these ADSCs with rabbit articular cartilage chondrocytes. We found that when ADSCs were cocultured with chondrocytes, the proliferation of articular cartilage chondrocytes was promoted, the apoptosis of chondrocytes was inhibited, and the osteogenic and chondrogenic differentiation of ADSCs was enhanced. The study on the mechanism of this coculture system indicated that the role of this coculture system is similar to the function of TGF-β1 in the promotion of chondrocytes.
authors.affiliation: Reconstructive and Plastic Surgery, The General Hospital of Shenyang Military Region, Shenyang, China.
authors.forename: Jie
authors.identifier: 0000-0002-1560-5729
authors.initials: J
authors.lastname: Shi
authors.affiliation: Reconstructive and Plastic Surgery, The General Hospital of Shenyang Military Region, Shenyang, China.
authors.forename: Jiulong
authors.identifier: 0000-0001-6335-4795
authors.initials: J
authors.lastname: Liang
authors.affiliation: Reconstructive and Plastic Surgery, The General Hospital of Shenyang Military Region, Shenyang, China.
authors.forename: Bingyu
authors.identifier: 0000-0002-8383-454X
authors.initials: B
authors.lastname: Guo
authors.affiliation: Reconstructive and Plastic Surgery, The General Hospital of Shenyang Military Region, Shenyang, China.
authors.forename: Yu
authors.identifier: 0000-0001-8660-331X
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Reconstructive and Plastic Surgery, The General Hospital of Shenyang Military Region, Shenyang, China.
authors.forename: Qiang
authors.identifier: 0000-0001-8245-6252
authors.initials: Q
authors.lastname: Hui
authors.affiliation: Reconstructive and Plastic Surgery, The General Hospital of Shenyang Military Region, Shenyang, China.
authors.forename: Peng
authors.identifier: 0000-0001-7926-7725
authors.initials: P
authors.lastname: Chang
authors.affiliation: Reconstructive and Plastic Surgery, The General Hospital of Shenyang Military Region, Shenyang, China.
authors.forename: Kai
authors.identifier: 0000-0002-9410-9669
authors.initials: K
authors.lastname: Tao