title: Design of a Bioabsorbable Multilayered Patch for Esophagus Tissue Engineering.
abstract: A gold standard for esophagus reconstruction is not still available. The present work aims to design a polymer patch combining synthetic polylactide-co-polycaprolacton and chitosan biopolymers, tailoring patch properties to esophageal tissue characteristics by a temperature-induced precipitation method, to get multilayered patches (1L, 2L, and 3L). Characterization shows stable multilayered patches (1L and 2L) by selection of copolymer type, and their Mw. In vitro investigation of the functional patch properties in simulated physiologic and pathologic conditions demonstrates that the chitosan layer (patch 3L) decreases patch stability and cell adhesion, while improves cell proliferation. Patches 2L and 3L comply with physiological esophageal pressure (3-5 kPa) and elongation (20%).
authors.affiliation: Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100, Pavia, Italy.
authors.forename: Rossella
authors.initials: R
authors.lastname: Dorati
authors.affiliation: Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100, Pavia, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: De Trizio
authors.affiliation: Department of Civil Engineering and Architecture, University of Pavia, Via Ferrata 1, 27100, Pavia, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Marconi
authors.affiliation: Department of Civil Engineering and Architecture, University of Pavia, Via Ferrata 1, 27100, Pavia, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Ferrara
authors.affiliation: Department of Civil Engineering and Architecture, University of Pavia, Via Ferrata 1, 27100, Pavia, Italy.
authors.forename: Ferdinando
authors.initials: F
authors.lastname: Auricchio
authors.affiliation: Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100, Pavia, Italy.
authors.forename: Ida
authors.initials: I
authors.lastname: Genta
authors.affiliation: Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100, Pavia, Italy.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Modena
authors.affiliation: Department of Otolaryngology/Head and Neck Surgery, University of Pavia, I.R.C.C.S. Policlinico S. Matteo, 27100, Pavia, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Benazzo
authors.affiliation: Department of Thoracic Surgery, Medical University of Vienna, 1090, Vienna, Austria.
authors.forename: Alberto
authors.initials: A
authors.lastname: Benazzo
authors.affiliation: Department of Otolaryngology/Head and Neck Surgery, University of Pavia, I.R.C.C.S. Policlinico S. Matteo, 27100, Pavia, Italy.
authors.forename: Gino
authors.initials: G
authors.lastname: Volpato
authors.affiliation: Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100, Pavia, Italy.
authors.forename: Bice
authors.initials: B
authors.lastname: Conti
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001704:Biopolymers / Q000737:chemistry* / Q000627:therapeutic use; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry / Q000627:therapeutic use; D004947:Esophagus / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D011091:Polyesters / Q000737:chemistry / Q000627:therapeutic use; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001704:Biopolymers; D011091:Polyesters; C016240:polycaprolactone; C033616:poly(lactide); D048271:Chitosan
keywords: biopolymers; chitosan; polylactide-co-polycaprolactone; scaffold; tissue regeneration