title: Polyphasic characterization of the thermotolerant cyanobacterium Desertifilum sp. strain IPPAS B-1220.
abstract: A cyanobacterial strain from Lake Shar-Nuur, a freshwater lake in Mongolia, was isolated and characterized by a polyphasic approach. According to the 16S ribosomal RNA gene sequence, this strain (IPPAS B-1220) belongs to a newly described genus Desertifilum. In general, strains of Desertifilum maintain their genetic stability, as seen from the analysis of the 16S rRNA gene and 16S-23S rRNA internal transcribed spacer sequences from strains collected at distant locations. The newly discovered strain is characterized by an unusual fatty acid composition (16:1Δ7 and 16:2Δ7,10). Analysis of its draft genomic sequence reveals the presence of six genes for the acyl-lipid desaturases: two Δ9-desaturases, desC1 and desC2; two Δ12-desaturases, desA1 and desA2; one desaturase of unknown specificity, desX; and one gene for the bacillary-type desaturase, desG, which supposedly encodes an ω9-desaturase. A scheme for a fatty acid desaturation pathway that describes the biosynthesis of 16:1Δ7 and 16:2Δ7,10 fatty acids in Desertifilum is proposed.
authors.affiliation: Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Sinetova
authors.affiliation: Department of Biotechnology, Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, Kazakhstan.
authors.forename: Kenzhegul
authors.initials: K
authors.lastname: Bolatkhan
authors.affiliation: Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia.
authors.forename: Roman A
authors.initials: RA
authors.lastname: Sidorov
authors.affiliation: Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia.
authors.forename: Kirill S
authors.initials: KS
authors.lastname: Mironov
authors.affiliation: Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia.
authors.forename: Alexandra N
authors.initials: AN
authors.lastname: Skrypnik
authors.affiliation: Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Kupriyanova
authors.affiliation: Department of Biotechnology, Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, Kazakhstan.
authors.forename: Bolatkhan K
authors.initials: BK
authors.lastname: Zayadan
authors.affiliation: Department of Biology, School of Natural Sciences, Kean University, 1000 Morris Ave, Union, NJ 07083, USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Shumskaya
authors.affiliation: Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia.
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Los
mesh_terms: D015373:Bacterial Typing Techniques; D000458:Cyanobacteria / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000502:physiology*; D004269:DNA, Bacterial / Q000235:genetics; D044943:Fatty Acid Desaturases / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005618:Fresh Water / Q000382:microbiology; D060106:Lakes / Q000382:microbiology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D000071436:Thermotolerance
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D044943:Fatty Acid Desaturases
keywords: Desertifilum; fatty acid desaturases; fatty acids; molecular phylogeny; polyphasic approach; ultrastructure