title: Adenovirus Vectors Target Several Cell Subtypes of Mammalian Inner EarIn Vivo.
abstract: Mammalian inner ear harbors diverse cell types that are essential for hearing and balance. Adenovirus is one of the major vectors to deliver genes into the inner ear for functional studies and hair cell regeneration. To identify adenovirus vectors that target specific cell subtypes in the inner ear, we studied three adenovirus vectors, carrying a reporter gene encoding green fluorescent protein(GFP)from two vendors or with a genome editing gene Cre recombinase(Cre), by injection into postnatal days 0 (P0) and 4 (P4) mouse cochlea through scala media by cochleostomyin vivo. We found three adenovirus vectors transduced mouse inner ear cells with different specificities and expression levels, depending on the type of adenoviral vectors and the age of mice. The most frequently targeted region was the cochlear sensory epithelium, including auditory hair cells and supporting cells. Adenovirus withGFPtransduced utricular supporting cells as well. This study shows that adenovirus vectors are capable of efficiently and specifically transducing different cell types in the mammalian inner ear and provides useful tools to study inner ear gene function and to evaluate gene therapy to treat hearing loss and vestibular dysfunction.
authors.affiliation: Department of Otolaryngology, Harvard Medical School and Eaton-Peabody Laboratories, Massachusetts Eye & Ear Infirmary, Boston, MA 02114, USA; Department of Otolaryngology-Head and Neck Surgery, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China; Key Laboratory of Hearing Medicine, National Health and Family Planning Commission, Shanghai, China.
authors.forename: Yilai
authors.identifier: 0000-0001-5668-5107
authors.initials: Y
authors.lastname: Shu
authors.affiliation: Department of Otolaryngology, Harvard Medical School and Eaton-Peabody Laboratories, Massachusetts Eye & Ear Infirmary, Boston, MA 02114, USA.
authors.forename: Yong
authors.identifier: 0000-0002-3346-6937
authors.initials: Y
authors.lastname: Tao
authors.affiliation: Department of Otolaryngology, Harvard Medical School and Eaton-Peabody Laboratories, Massachusetts Eye & Ear Infirmary, Boston, MA 02114, USA; Department of Otolaryngology-Head and Neck Surgery, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China; Key Laboratory of Hearing Medicine, National Health and Family Planning Commission, Shanghai, China.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA; Laboratory for Molecular Medicine, Partners Personalized Medicine, Cambridge, MA 02139, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Shen
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China; Key Laboratory of Hearing Medicine, National Health and Family Planning Commission, Shanghai, China.
authors.forename: Zhengmin
authors.identifier: 0000-0002-5141-9851
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Otolaryngology, Harvard Medical School and Eaton-Peabody Laboratories, Massachusetts Eye & Ear Infirmary, Boston, MA 02114, USA.
authors.forename: Zheng-Yi
authors.identifier: 0000-0002-1452-4193
authors.initials: ZY
authors.lastname: Chen
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D000831:Animals, Newborn; D003051:Cochlea / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D007758:Ear, Inner / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D018390:Gene Targeting / Q000379:methods*; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000008:administration & dosage / Q000235:genetics; D051379:Mice
chemical_list: D049452:Green Fluorescent Proteins