title: HLA class II alleles and risk for peripheral neuropathy in type 2 diabetes patients.
abstract: The potential impact of human leukocyte antigen (HLA) genotype variations on development of diabetic peripheral neuropathy (DPN) is not well determined. This study aimed to identify the association of HLA class II alleles with DPN in type 2 diabetes (T2D) patients. Totally 106 T2D patients, 49 with DPN and 57 without DPN, and 100 ethnic-matched healthy controls were analyzed. Both groups of the patients were matched based on sex, age, body mass index (BMI) and duration of T2D. Polyneuropathy was diagnosed using electrodiagnostic methods. HLA-DRB1 and DQB1 genotyping was performed in all subjects by the polymerase chain reaction with sequence-specific primers (PCR-SSP) method. T2D patients with DPN showed higher frequencies of HLA-DRB1*10 and DRB1*12 alleles compared to control group (P= 0.04). HLA-DQB1*02 allele and HLA-DRB1*07-DQB1*02 haplotype were associated with a decreased risk for developing DPN in T2D patients (P= 0.02 andP= 0.05 respectively). Also, patients with severe neuropathy showed higher frequencies of DRB1*07 (P= 0.003) and DQB1*02 (P= 0.02) alleles than those with mild-to-moderate form of neuropathy. The distribution of DRB1 and DQB1 alleles and haplotypes were not statistically different between all patients and healthy controls. Our findings implicate a possible protective role of HLA-DQB1*02 allele and HLA-DRB1*07-DQB1*02 haplotype against development of peripheral neuropathy in T2D patients. Therefore, variations in HLA genotypes might be used as genetic markers for prediction and potentially management of neuropathy in T2D patients.
authors.affiliation: Division of Endocrinology, Department of Internal Medicine, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Marzban
authors.affiliation: Division of Endocrinology, Department of Internal Medicine, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Javad
authors.initials: J
authors.lastname: Kiani
authors.affiliation: Department of Immunology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Hajilooi
authors.affiliation: Department of Immunology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Hamzeh
authors.initials: H
authors.lastname: Rezaei
authors.affiliation: Division of Endocrinology, Department of Internal Medicine, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Kahramfar
authors.affiliation: Department of Immunology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran; Psoriasis Research Center, Department of Dermatology, Farshchian Hospital, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Ghasem
authors.identifier: 0000-0001-8929-5658
authors.initials: G
authors.lastname: Solgi
keywords: HLA-DQB1; HLA-DRB1; alleles; genotypes; haplotypes; nerve regeneration; neural regeneration; peripheral neuropathy; type 2 diabetes