title: Novel adverse outcome pathways revealed by chemical genetics in a developing marine fish.
abstract: Crude oil spills are a worldwide ocean conservation threat. Fish are particularly vulnerable to the oiling of spawning habitats, and crude oil causes severe abnormalities in embryos and larvae. However, the underlying mechanisms for these developmental defects are not well understood. Here, we explore the transcriptional basis for four discrete crude oil injury phenotypes in the early life stages of the commercially important Atlantic haddock (Melanogrammus aeglefinus). These include defects in (1) cardiac form and function, (2) craniofacial development, (3) ionoregulation and fluid balance, and (4) cholesterol synthesis and homeostasis. Our findings suggest a key role for intracellular calcium cycling and excitation-transcription coupling in the dysregulation of heart and jaw morphogenesis. Moreover, the disruption of ionoregulatory pathways sheds new light on buoyancy control in marine fish embryos. Overall, our chemical-genetic approach identifies initiating events for distinct adverse outcome pathways and novel roles for individual genes in fundamental developmental processes.
authors.affiliation: Institute of Marine Research, Bergen, Norway.|Centre for Ecological and Evolutionary Synthesis, University of Oslo, Oslo, Norway.
authors.forename: Elin
authors.identifier: 0000-0003-3542-4201
authors.initials: E
authors.lastname: Sørhus
authors.affiliation: Environmental and Fisheries Science Division, Northwest Fisheries Science Center, National Marine Fisheries Service, Seattle, United States.
authors.forename: John P
authors.initials: JP
authors.lastname: Incardona
authors.affiliation: Institute of Marine Research, Bergen, Norway.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Furmanek
authors.affiliation: Environmental and Fisheries Science Division, Northwest Fisheries Science Center, National Marine Fisheries Service, Seattle, United States.
authors.forename: Giles W
authors.initials: GW
authors.lastname: Goetz
authors.affiliation: Environmental and Fisheries Science Division, Northwest Fisheries Science Center, National Marine Fisheries Service, Seattle, United States.
authors.forename: Nathaniel L
authors.initials: NL
authors.lastname: Scholz
authors.affiliation: Institute of Marine Research, Bergen, Norway.
authors.forename: Sonnich
authors.initials: S
authors.lastname: Meier
authors.affiliation: Institute of Marine Research, Bergen, Norway.
authors.forename: Rolf B
authors.initials: RB
authors.lastname: Edvardsen
authors.affiliation: Centre for Ecological and Evolutionary Synthesis, University of Oslo, Oslo, Norway.|Department of Natural Sciences, University of Agder, Kristiansand, Norway.
authors.forename: Sissel
authors.initials: S
authors.lastname: Jentoft
mesh_terms: D000818:Animals; D059001:Aquatic Organisms / Q000187:drug effects*; D048388:Gadiformes / Q000196:embryology*; D009024:Morphogenesis / Q000187:drug effects*; D010578:Petroleum / Q000633:toxicity*; D014873:Water Pollutants / Q000633:toxicity*
chemical_list: D010578:Petroleum; D014873:Water Pollutants
keywords: Atlantic haddock; cardiac abnormalities; chemical genetics; craniofacial abnormalities; crude oil; ecology; evolutionary biology; genomics; transcriptome