title: Characteristics Peripheral Blood IgG and IgM Heavy Chain Complementarity Determining Region 3 Repertoire before and after Immunization with Recombinant HBV Vaccine.
abstract: Immunization with recombinant HBV vaccine induces specific immune responses in human causing B lymphocytes to produce protective HBsAb, and to form memory B lymphocytes, thereby facilitating HBV immunity in the body. However, B lymphocytes heterogeneity and characteristics are not fully elucidated. In this study, we conducted high-throughput sequencing of BCR heavy chain CDR3 repertoires in 3 healthy volunteers before and after the third immunization with recombinant HBV vaccine. We used Roche 454 Genome Sequencer FLX system to perform a comparative analysis of IgM and IgG H chain CDR3 repertoires. First, we found that the diversity of IgG H chain CDR3 repertoires was 1/6 of IgM on average. Moreover, after the third immunization with HBV vaccine, the diversity of IgG H chain CDR3 repertoires was 1/26 of IgM on average. Second, we detected relatively high levels of HBsAbs in all the healthy volunteers after immunization with HBV vaccine. The volunteers shared a small number of CDR3 sequences before and after immunization, and among each other. However, we did not find completely identical BCR H chain CDR3 amino acid sequences in these volunteers. Lastly, after immunization with recombinant HBV vaccine, the volunteers showed high frequency of IgG H chain CDR3 amino acid sequences mostly resulting from rearrangements of IGHV, IGHD and IGHJ, suggesting that the mechanism of high frequency CDR3 generation might be associated with the maturation of IgG affinity (somatic hypermutation) during the recombinant HBV vaccine-induced B lymphocyte responses. This study identified the characteristics and changes of BCR CDR3 repertoires before and after immunization with HBV vaccine, and evaluated the performance of the sequencing technology for this application. Our findings provide a basis for further research in B lymphocyte generated HBsAb heterogeneity and monitoring the maintenance of memory B lymphocytes.
authors.affiliation: Department of Immunology, Research Center for Medicine & Biology, Innovation & Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi, Guizhou, China.
authors.forename: Long
authors.initials: L
authors.lastname: Ma
authors.affiliation: The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Immunology, Research Center for Medicine & Biology, Innovation & Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi, Guizhou, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Bi
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Jiezuan
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Laboratory Medicine, Zunyi Medical University, Zunyi, Guizhou, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Shi
authors.affiliation: Department of Immunology, Research Center for Medicine & Biology, Innovation & Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi, Guizhou, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Immunology, Research Center for Medicine & Biology, Innovation & Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi, Guizhou, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Ma
authors.affiliation: Department of Immunology, Research Center for Medicine & Biology, Innovation & Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi, Guizhou, China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Department of Immunology, Research Center for Medicine & Biology, Innovation & Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi, Guizhou, China.
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Yao
mesh_terms: D022801:Complementarity Determining Regions; D064368:Healthy Volunteers; D017325:Hepatitis B Vaccines / Q000008:administration & dosage*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000737:chemistry; D007075:Immunoglobulin M / Q000097:blood* / Q000737:chemistry; D014614:Vaccines, Synthetic / Q000008:administration & dosage*
chemical_list: D022801:Complementarity Determining Regions; D017325:Hepatitis B Vaccines; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014614:Vaccines, Synthetic