title: Bone Marrow CD34+Progenitor Cells from HIV-Infected Patients Show an Impaired T Cell Differentiation Potential Related to Proinflammatory Cytokines.
abstract: The impact of HIV infection on the frequency and differentiation capability of CD34+bone marrow hematopoietic progenitor cells (BM-HPCs) is still debated, having a possible primary role in antiretroviral-induced immunoreconstitution. We investigated the influence of HIV replication or proinflammatory cytokines on lymphopoietic capability of BM-HPCs from seven viremic (VR) and five nonviremic (NVR) HIV-infected patients. We found that BM-HPCs from VR patients were unable to differentiate in vitro toward T cells, and produced proinflammatory cytokines in the absence of viral replication. In contrast, the lymphoid differentiation potential of BM-HPCs was partially restored in successfully antiretroviral therapy-treated patients. We also showed that TLR8 triggering induced BM-HPCs from healthy donors to release proinflammatory cytokines affecting T cell differentiation. These data suggest that in HIV-infected patients, the lymphopoiesis capability of BM-HPCs may be modulated by a virus-driven autocrine mechanism involving proinflammatory cytokines.
authors.affiliation: 1 Cellular Immunology Laboratory, National Institute for Infectious Diseases "L. Spallanzani" I.R.C.C.S. , Rome, Italy .
authors.forename: Veronica
authors.initials: V
authors.lastname: Bordoni
authors.affiliation: 2 Clinical Department, National Institute for Infectious Diseases "L. Spallanzani" I.R.C.C.S. , Rome, Italy .
authors.forename: Michele
authors.initials: M
authors.lastname: Bibas
authors.affiliation: 1 Cellular Immunology Laboratory, National Institute for Infectious Diseases "L. Spallanzani" I.R.C.C.S. , Rome, Italy .
authors.forename: Domenico
authors.initials: D
authors.lastname: Viola
authors.affiliation: 1 Cellular Immunology Laboratory, National Institute for Infectious Diseases "L. Spallanzani" I.R.C.C.S. , Rome, Italy .
authors.forename: Alessandra
authors.initials: A
authors.lastname: Sacchi
authors.affiliation: 1 Cellular Immunology Laboratory, National Institute for Infectious Diseases "L. Spallanzani" I.R.C.C.S. , Rome, Italy .
authors.forename: Eleonora
authors.initials: E
authors.lastname: Cimini
authors.affiliation: 1 Cellular Immunology Laboratory, National Institute for Infectious Diseases "L. Spallanzani" I.R.C.C.S. , Rome, Italy .
authors.forename: Nicola
authors.initials: N
authors.lastname: Tumino
authors.affiliation: 1 Cellular Immunology Laboratory, National Institute for Infectious Diseases "L. Spallanzani" I.R.C.C.S. , Rome, Italy .
authors.forename: Rita
authors.initials: R
authors.lastname: Casetti
authors.affiliation: 3 Virology Laboratory, National Institute for Infectious Diseases "L. Spallanzani" I.R.C.C.S. , Rome, Italy .
authors.forename: Alessandra
authors.initials: A
authors.lastname: Amendola
authors.affiliation: 2 Clinical Department, National Institute for Infectious Diseases "L. Spallanzani" I.R.C.C.S. , Rome, Italy .
authors.forename: Adriana
authors.initials: A
authors.lastname: Ammassari
authors.affiliation: 1 Cellular Immunology Laboratory, National Institute for Infectious Diseases "L. Spallanzani" I.R.C.C.S. , Rome, Italy .|3 Virology Laboratory, National Institute for Infectious Diseases "L. Spallanzani" I.R.C.C.S. , Rome, Italy .
authors.forename: Chiara
authors.initials: C
authors.lastname: Agrati
authors.affiliation: 1 Cellular Immunology Laboratory, National Institute for Infectious Diseases "L. Spallanzani" I.R.C.C.S. , Rome, Italy .
authors.forename: Federico
authors.initials: F
authors.lastname: Martini
mesh_terms: D018952:Antigens, CD34 / Q000032:analysis*; D001853:Bone Marrow / Q000473:pathology*; D002454:Cell Differentiation; D016207:Cytokines / Q000378:metabolism*; D015658:HIV Infections / Q000473:pathology*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D013601:T-Lymphocytes / Q000502:physiology*
chemical_list: D018952:Antigens, CD34; D016207:Cytokines
keywords: HIV; T cells; immune response; in vitro models; inflammation