title: Bacteria encapsulated electrospun nanofibrous webs for remediation of methylene blue dye in water.
abstract: In this study, preparation and application of novel biocomposite materials that were produced by encapsulation of bacterial cells within electrospun nanofibrous webs are described. A commercial strain of Pseudomonas aeruginosa which has methylene blue (MB) dye remediation capability was selected for encapsulation, and polyvinyl alcohol (PVA) and polyethylene oxide (PEO) were selected as the polymer matrices for the electrospinning of bacteria encapsulated nanofibrous webs. Encapsulation of bacterial cells was monitored by scanning electron microscopy (SEM) and fluorescence microscopy, and the viability of encapsulated bacteria was checked by live/dead staining and viable cell counting assay. Both bacteria/PVA and bacteria/PEO webs have shown a great potential for remediation of MB, yet bacteria/PEO web has shown higher removal performances than bacteria/PVA web, which was probably due to the differences in the initial viable bacterial cells for those two samples. The bacteria encapsulated electrospun nanofibrous webs were stored at 4°C for three months and they were found as potentially storable for keeping encapsulated bacterial cells alive. Overall, the results suggest that electrospun nanofibrous webs are suitable platforms for preservation of living bacterial cells and they can be used directly as a starting inoculum for bioremediation of water systems.
authors.affiliation: Institute of Materials Science & Nanotechnology, Bilkent University, 06800, Bilkent, Ankara, Turkey; UNAM-National Nanotechnology Research Center, Bilkent University, 06800, Bilkent, Ankara, Turkey.
authors.forename: Omer Faruk
authors.initials: OF
authors.lastname: Sarioglu
authors.affiliation: UNAM-National Nanotechnology Research Center, Bilkent University, 06800, Bilkent, Ankara, Turkey; Polatlı Science and Literature Faculty, Biology Department, Gazi University, 06900, Polatlı, Ankara, Turkey; Life Sciences Application and Research Center, Gazi University, 06830, Golbasi, Ankara, Turkey.
authors.forename: Nalan Oya San
authors.initials: NOS
authors.lastname: Keskin
authors.affiliation: Institute of Materials Science & Nanotechnology, Bilkent University, 06800, Bilkent, Ankara, Turkey; UNAM-National Nanotechnology Research Center, Bilkent University, 06800, Bilkent, Ankara, Turkey.
authors.forename: Asli
authors.initials: A
authors.lastname: Celebioglu
authors.affiliation: Life Sciences Application and Research Center, Gazi University, 06830, Golbasi, Ankara, Turkey; Department of Medical Biology and Genetics, Faculty of Medicine, Gazi University, 06500, Besevler, Ankara, Turkey. Electronic address: ttekinay@gazi.edu.tr.
authors.forename: Turgay
authors.initials: T
authors.lastname: Tekinay
authors.affiliation: Institute of Materials Science & Nanotechnology, Bilkent University, 06800, Bilkent, Ankara, Turkey; UNAM-National Nanotechnology Research Center, Bilkent University, 06800, Bilkent, Ankara, Turkey. Electronic address: uyar@unam.bilkent.edu.tr.
authors.forename: Tamer
authors.initials: T
authors.lastname: Uyar
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D008751:Methylene Blue / Q000378:metabolism*; D057139:Nanofibers / Q000382:microbiology*; D014867:Water / Q000378:metabolism
chemical_list: D014867:Water; D008751:Methylene Blue
keywords: Bacteria; Bioremediation; Electrospinning; Encapsulation; Methylene blue; Nanofibers