title: Lipidomics reveals dramatic lipid compositional changes in the maturing postnatal lung.
abstract: Lung immaturity is a major cause of morbidity and mortality in premature infants. Understanding the molecular mechanisms driving normal lung development could provide insights on how to ameliorate disrupted development. While transcriptomic and proteomic analyses of normal lung development have been previously reported, characterization of changes in the lipidome is lacking. Lipids play significant roles in the lung, such as dipalmitoylphosphatidylcholine in pulmonary surfactant; however, many of the roles of specific lipid species in normal lung development, as well as in disease states, are not well defined. In this study, we used liquid chromatography-mass spectrometry (LC-MS/MS) to investigate the murine lipidome during normal postnatal lung development. Lipidomics analysis of lungs from post-natal day 7, day 14 and 6-8 week mice (adult) identified 924 unique lipids across 21 lipid subclasses, with dramatic alterations in the lipidome across developmental stages. Our data confirmed previously recognized aspects of post-natal lung development and revealed several insights, including in sphingolipid-mediated apoptosis, inflammation and energy storage/usage. Complementary proteomics, metabolomics and chemical imaging corroborated these observations. This multi-omic view provides a unique resource and deeper insight into normal pulmonary development.
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, USA.
authors.forename: Sydney E
authors.initials: SE
authors.lastname: Dautel
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, USA.
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Kyle
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, USA.
authors.forename: Geremy
authors.initials: G
authors.lastname: Clair
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, USA.
authors.forename: Ryan L
authors.initials: RL
authors.lastname: Sontag
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, USA.
authors.forename: Karl K
authors.initials: KK
authors.lastname: Weitz
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, USA.
authors.forename: Anil K
authors.initials: AK
authors.lastname: Shukla
authors.affiliation: Physical Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, USA.
authors.forename: Son N
authors.initials: SN
authors.lastname: Nguyen
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, USA.
authors.forename: Young-Mo
authors.initials: YM
authors.lastname: Kim
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, USA.
authors.forename: Erika M
authors.initials: EM
authors.lastname: Zink
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, USA.
authors.forename: Teresa
authors.initials: T
authors.lastname: Luders
authors.affiliation: Center for Lung Biology, University of Washington, Seattle, Washington, USA.
authors.forename: Charles W
authors.initials: CW
authors.lastname: Frevert
authors.affiliation: Center for Lung Biology, University of Washington, Seattle, Washington, USA.
authors.forename: Sina A
authors.initials: SA
authors.lastname: Gharib
authors.affiliation: Physical Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, USA.
authors.forename: Julia
authors.initials: J
authors.lastname: Laskin
authors.affiliation: Texas Advanced Computing Center, University of Texas at Austin, Austin, TX 78758, USA.
authors.forename: James P
authors.initials: JP
authors.lastname: Carson
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, USA.
authors.forename: Thomas O
authors.initials: TO
authors.lastname: Metz
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, USA.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Corley
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, USA.
authors.forename: Charles
authors.initials: C
authors.lastname: Ansong
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D005227:Fatty Acids / Q000378:metabolism; D007249:Inflammation / Q000473:pathology; D050356:Lipid Metabolism; D008168:Lung / Q000254:growth & development* / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D008810:Mice, Inbred C57BL; D011650:Pulmonary Alveoli / Q000254:growth & development; D013107:Sphingolipids / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D013107:Sphingolipids