title: Comparison of a commercial real-time PCR assay, RealCycler® PJIR kit, progenie molecular, to an in-house real-time PCR assay for the diagnosis of Pneumocystis jirovecii infections.
abstract: We compared the RealCycler® PJIR kit (Progenie Molecular), available in Europe, to an in-house real-time PCR assay for the diagnosis of Pneumocystis jirovecii infections. Excellent agreement was found (concordance rate, 97.4%; Cohen's kappa, 0.918>0.8) showing that this commercial assay represents an alternative method for the diagnosis of P. jirovecii infections.
authors.affiliation: Laboratory of Parasitology and Mycology, Brest University Hospital, Brest, France; University of Brest, GEIHP EA, 3142, Brest, France. Electronic address: thibaud.guillaud-saumur@hotmail.fr.
authors.forename: Thibaud
authors.initials: T
authors.lastname: Guillaud-Saumur
authors.affiliation: Laboratory of Parasitology and Mycology, Brest University Hospital, Brest, France; University of Brest, GEIHP EA, 3142, Brest, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Nevez
authors.affiliation: Medical intensive care unit, Brest University Hospital, Brest, France.
authors.forename: Amélie
authors.initials: A
authors.lastname: Bazire
authors.affiliation: University of Brest, GEIHP EA, 3142, Brest, France.
authors.forename: Michèle
authors.initials: M
authors.lastname: Virmaux
authors.affiliation: University of Angers, GEIHP EA, 3142, Angers, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Papon
authors.affiliation: Laboratory of Parasitology and Mycology, Brest University Hospital, Brest, France; University of Brest, GEIHP EA, 3142, Brest, France. Electronic address: solene.legal@univ-brest.fr.
authors.forename: Solène
authors.initials: S
authors.lastname: Le Gal
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001681:Biological Assay / Q000379:methods; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D005060:Europe; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D045363:Pneumocystis carinii / Q000235:genetics* / Q000302:isolation & purification*; D011020:Pneumonia, Pneumocystis / Q000175:diagnosis* / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
keywords: Commercial kit; Comparison of methods; Pneumocystis jirovecii; Real-time PCR