title: Drought stress had a predominant effect over heat stress on three tomato cultivars subjected to combined stress.
abstract: Abiotic stresses due to environmental factors could adversely affect the growth and development of crops. Among the abiotic stresses, drought and heat stress are two critical threats to crop growth and sustainable agriculture worldwide. Considering global climate change, incidence of combined drought and heat stress is likely to increase. The aim of this study was to shed light on plant growth performance and leaf physiology of three tomatoes cultivars ('Arvento', 'LA1994' and 'LA2093') under control, drought, heat and combined stress.
  
  
  
  Shoot fresh and dry weight, leaf area and relative water content of all cultivars significantly decreased under drought and combined stress as compared to control. The net photosynthesis and starch content were significantly lower under drought and combined stress than control in the three cultivars. Stomata and pore length of the three cultivars significantly decreased under drought and combined stress as compared to control. The tomato 'Arvento' was more affected by heat stress than 'LA1994' and 'LA2093' due to significant decreases in shoot dry weight, chlorophyll a and carotenoid content, starch content and NPQ (non-photochemical quenching) only in 'Arvento' under heat treatment. By comparison, the two heat-tolerant tomatoes were more affected by drought stress compared to 'Arvento' as shown by small stomatal and pore area, decreased sucrose content, ΦPSII(quantum yield of photosystem II), ETR (electron transport rate) and qL(fraction of open PSII centers) in 'LA1994' and 'LA2093'. The three cultivars showed similar response when subjected to the combination of drought and heat stress as shown by most physiological parameters, even though only 'LA1994' and 'LA2093' showed decreased Fv/Fm(maximum potential quantum efficiency of photosystem II), ΦPSII, ETR and qLunder combined stress.
  
  
  
  The cultivars differing in heat sensitivity did not show difference in the combined stress sensitivity, indicating that selection for tomatoes with combined stress tolerance might not be correlated with the single stress tolerance. In this study, drought stress had a predominant effect on tomato over heat stress, which explained why simultaneous application of heat and drought revealed similar physiological responses to the drought stress. These results will uncover the difference and linkage between the physiological response of tomatoes to drought, heat and combined stress and be important for the selection and breeding of tolerant tomato cultivars under single and combine stress.
authors.affiliation: Institute of Vegetable Crop, Jiangsu Academy of Agricultural Science, Nanjing, Jiangsu, China.|Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing, Jiangsu, China.|College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhou
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Xiaqing
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Food Science, Aarhus University, Årslev, Denmark.
authors.forename: Carl-Otto
authors.initials: CO
authors.lastname: Ottosen
authors.affiliation: Department of Plant and Environmental Sciences, University of Copenhagen, Taastrup, Denmark.
authors.forename: Eva
authors.initials: E
authors.lastname: Rosenqvist
authors.affiliation: Institute of Vegetable Crop, Jiangsu Academy of Agricultural Science, Nanjing, Jiangsu, China.|Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing, Jiangsu, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Institute of Vegetable Crop, Jiangsu Academy of Agricultural Science, Nanjing, Jiangsu, China.|Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing, Jiangsu, China.
authors.forename: Yinlei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Vegetable Crop, Jiangsu Academy of Agricultural Science, Nanjing, Jiangsu, China.|Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing, Jiangsu, China.
authors.forename: Wengui
authors.initials: W
authors.lastname: Yu
authors.affiliation: Institute of Vegetable Crop, Jiangsu Academy of Agricultural Science, Nanjing, Jiangsu, China. tmzhaomail@163.com.|Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing, Jiangsu, China. tmzhaomail@163.com.
authors.forename: Tongmin
authors.initials: T
authors.lastname: Zhao
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, China. zpzx@njau.edu.cn.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wu
mesh_terms: D000064:Acclimatization; D050260:Carbohydrate Metabolism; D002734:Chlorophyll / Q000378:metabolism; D055864:Droughts; D018869:Heat-Shock Response; D006358:Hot Temperature; D018551:Solanum lycopersicum / Q000254:growth & development / Q000502:physiology*; D010858:Pigmentation; D018515:Plant Leaves / Q000502:physiology; D054046:Plant Stomata / Q000378:metabolism; D013045:Species Specificity; D014867:Water / Q000378:metabolism
chemical_list: D014867:Water; D002734:Chlorophyll
keywords: Chlorophyll fluorescence; Drought stress; Heat stress; Photosynthesis; Tomato