title: Pectic Oligosaccharide from tomato exhibiting anticancer potential on a gastric cancer cell line: Structure-function relationship.
abstract: Pectic Polysaccharide (PP) from dietary sources has been known to prevent cancer growth and hence impede cancer progression. We evaluated anticancer effect of Pectic-Oligosaccharide isolated from Sour Raw Tomato (SrTPO); its bioavailability and structure elucidated from purified fraction (SrTPO1). SrTPO1 inhibited galectin-3 activity with MIC of 0.25 ug/mL (100 fold better than standard galactose), inhibited the growth of AGS cells (IC503.4μg/mL) and induced apoptosis (70% inhibition at 30μg/mL concentration). Normal- NIH 3T3 cells were not affected by SrTPO as opposed to doxorubicin, a known anticancer drug, which reduced 76% viability at equivalent dose. SrTPO1 was identified as RhamnogalacturonanI-arabinogalactan (RGI-AG), where repeated alternative rhamnose and galacturonic acid residues were observed while arabinose in the branch point and β-1,4 linked galactose in the linear chain form. SrTPO was found to be bioavailable as evaluated by FITC labelled oligos inside the cell, which was in reciprocal proportion with apoptosis.
authors.affiliation: Department of Biochemistry, CSIR-Central Food Technological Research Institute, Mysore-570020, India.
authors.forename: Sabeeta
authors.initials: S
authors.lastname: Kapoor
authors.affiliation: Department of Biochemistry, CSIR-Central Food Technological Research Institute, Mysore-570020, India. Electronic address: shylaja@cftri.res.in.
authors.forename: Shylaja M
authors.initials: SM
authors.lastname: Dharmesh
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis; D045744:Cell Line, Tumor; D006801:Humans; D018551:Solanum lycopersicum / Q000737:chemistry*; D009844:Oligosaccharides / Q000494:pharmacology*; D010368:Pectins / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000188:drug therapy; D013329:Structure-Activity Relationship
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D009844:Oligosaccharides; D010368:Pectins
keywords: Bioavailability; Gastric cancer; Pectic polysaccharide; Pectic-oligosaccharide; Rahmnogalactouronanl-arabinogalactan; Sour raw tomato