title: Muscadine Grape (Vitis rotundifolia) or Wine Phytochemicals Reduce Intestinal Inflammation in Mice with Dextran Sulfate Sodium-Induced Colitis.
abstract: The objective of this study was to determine the anti-inflammatory effects of phytochemical extracts from muscadine grapes or wine on dextran sulfate sodium (DSS)-induced colitis in mice and to investigate cellular mechanisms. Two groups of C57BL/6J mice were gavaged with muscadine grape phytochemicals (MGP) or muscadine wine phytochemicals (MWP), respectively, for 14 days. Acute colitis was induced by 3% DSS in drinking water for 7 days. An additional two groups of mice served as healthy and disease controls. Results indicated that MGP or MWP significantly prevented weight loss, reduced disease activity index, and preserved colonic length compared to the colitis group (p ≤ 0.05). MGP or MWP significantly decreased myeloperoxidase activity as well as the levels of IL-1β, IL-6, and TNF-α in colon (p ≤ 0.05). MGP or MWP caused down-regulation of the NF-κB pathway by inhibiting the phosphorylation and degradation of IκB in a dose-dependent manner. These findings suggest that phytochemicals from muscadine grape or wine mitigate ulcerative colitis via attenuation of pro-inflammatory cytokine production and modulation of the NF-κB pathway.
authors.affiliation: Food Science and Human Nutrition Department and ‡Center for Nutritional Sciences, College of Agricultural and Life Sciences, University of Florida , Gainesville, Florida 32611, United States.
authors.forename: Ruiqi
authors.identifier: http://orcid.org/0000-0001-8206-0362
authors.initials: R
authors.lastname: Li
authors.affiliation: Food Science and Human Nutrition Department and ‡Center for Nutritional Sciences, College of Agricultural and Life Sciences, University of Florida , Gainesville, Florida 32611, United States.
authors.forename: Min-Hyun
authors.initials: MH
authors.lastname: Kim
authors.affiliation: Food Science and Human Nutrition Department and ‡Center for Nutritional Sciences, College of Agricultural and Life Sciences, University of Florida , Gainesville, Florida 32611, United States.
authors.forename: Amandeep K
authors.initials: AK
authors.lastname: Sandhu
authors.affiliation: Food Science and Human Nutrition Department and ‡Center for Nutritional Sciences, College of Agricultural and Life Sciences, University of Florida , Gainesville, Florida 32611, United States.
authors.forename: Chi
authors.initials: C
authors.lastname: Gao
authors.affiliation: Food Science and Human Nutrition Department and ‡Center for Nutritional Sciences, College of Agricultural and Life Sciences, University of Florida , Gainesville, Florida 32611, United States.
authors.forename: Liwei
authors.initials: L
authors.lastname: Gu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology; D003106:Colon / Q000187:drug effects / Q000276:immunology; D016264:Dextran Sulfate / Q000009:adverse effects; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D020672:I-kappa B Proteins / Q000235:genetics / Q000276:immunology; D053583:Interleukin-1beta / Q000235:genetics / Q000276:immunology; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology; D007422:Intestines / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology; D010936:Plant Extracts / Q000008:administration & dosage*; D027843:Vitis / Q000737:chemistry*; D014920:Wine / Q000032:analysis*
chemical_list: D000893:Anti-Inflammatory Agents; D020672:I-kappa B Proteins; D053583:Interleukin-1beta; D015850:Interleukin-6; D016328:NF-kappa B; D010936:Plant Extracts; D016264:Dextran Sulfate
keywords: NF-κB; intestinal inflammation; muscadine grape; phytochemicals; wine