title: Molecular evidence of tick-borne hemoprotozoan-parasites (Theileria ovis and Babesia ovis) and bacteria in ticks and blood from small ruminants in Northern Algeria.
abstract: Using qPCR, standard PCR and/or sequencing, we investigated the presence of tick-associated microorganisms in ticks and blood from sheep and goats from Souk Ahras, Algeria. Borrelia theileri, was detected in (7/120, 5.8%) blood from sheep and (13/120, 10.8%) goats. Anaplasma ovis was screened in (38/73, 52%) Rhipicephalus bursa and (5/22, 22.7%) R. turanicus and in (74/120, 61.7%), (65/120, 54.2%) blood of sheep and goats respectively. Coxiella burnetii tested positive in R. bursa (4/73, 5.5%) and (7/120, 5.8%) blood of sheep and (2/120, 1.7%) goats. Theileria ovis was detected in (50/147, 34%) R. bursa and (3/22, 13.6%) R. turanicus and in (64/120, 53.3%) blood of sheep and (25/120, 20.8%) goats. Babesia ovis was screened positive in (23/147, 15.6%) R. bursa and (7/48, 14.6%) R. turanicus. Our findings expand knowledge about the repertoire of tick-borne microorganisms present in ectoparasites and/or the blood of small ruminants in Algeria.
authors.affiliation: Université Chadli Bendjdid, Département des Sciences Vétérinaires, El Tarf, 36000, Algeria; Université Mohamed Cherif Messaadia, Institut des Sciences Agronomiques et Vétérinaires, Souk Ahras, 41000, Algeria.
authors.forename: Atef
authors.initials: A
authors.lastname: Aouadi
authors.affiliation: Aix Marseille Université, Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63, CNRS 7278, IRD 198 (Dakar), Inserm 1095, Marseille, France; Ecole Nationale Supérieure Vétérinaire d'Alger. El Aliya Alger, 16000, Algeria.
authors.forename: Hamza
authors.initials: H
authors.lastname: Leulmi
authors.affiliation: Université Chadli Bendjdid, Département des Sciences Vétérinaires, El Tarf, 36000, Algeria.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Boucheikhchoukh
authors.affiliation: Université Chadli Bendjdid, Département des Sciences Vétérinaires, El Tarf, 36000, Algeria.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Benakhla
authors.affiliation: Aix Marseille Université, Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63, CNRS 7278, IRD 198 (Dakar), Inserm 1095, Marseille, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Raoult
authors.affiliation: Aix Marseille Université, Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63, CNRS 7278, IRD 198 (Dakar), Inserm 1095, Marseille, France. Electronic address: philippe.parola@univ-amu.fr.
authors.forename: Philippe
authors.initials: P
authors.lastname: Parola
mesh_terms: D000462:Algeria / Q000453:epidemiology; D042323:Anaplasma ovis / Q000235:genetics / Q000302:isolation & purification; D000712:Anaplasmosis / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology; D000818:Animals; D001403:Babesia / Q000235:genetics / Q000302:isolation & purification; D001404:Babesiosis / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology; D001898:Borrelia / Q000235:genetics / Q000302:isolation & purification; D001899:Borrelia Infections / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology; D016997:Coxiella burnetii / Q000235:genetics / Q000302:isolation & purification; D003430:Cross-Sectional Studies; D015511:Goat Diseases / Q000175:diagnosis / Q000382:microbiology / Q000469:parasitology; D006041:Goats / Q000469:parasitology; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000302:isolation & purification*; D016792:Piroplasmida / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011778:Q Fever / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary; D048494:Rhipicephalus / Q000382:microbiology* / Q000469:parasitology*; D012418:Ruminants / Q000382:microbiology* / Q000469:parasitology*; D012756:Sheep / Q000469:parasitology; D012757:Sheep Diseases / Q000175:diagnosis / Q000382:microbiology / Q000469:parasitology; D016794:Theileria / Q000235:genetics / Q000302:isolation & purification; D013801:Theileriasis / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology; D017282:Tick-Borne Diseases / Q000453:epidemiology / Q000382:microbiology / Q000469:parasitology / Q000662:veterinary*
keywords: Algeria; Babesia ovis; Small ruminants; Theileria ovis; Ticks