title: [Effects of MMPs inhibitors on microleakage with wet bonding technique].
abstract: OBJECTIVE
  To observe the effects of matrix metalloproteinases(MMPs) inhibitors on microleakage with wet bonding technique.
  
  
  METHODS
  Twenty-seven premolars were randomly assigned to 3 groups. Class Ⅴ cavities were prepared in the neck of the teeth and treated with distilled water, 2% chlorhexidine (CHX) or 2% monocycline (MI) for 60 seconds. Resin was used to restore the prepared cavities following application of luting agent. Samples in each group were soaked for 1 h with 10% NaClO and then 2 layers of nail polish were applied, followed by treatment with 50wt% ammoniated silver nitrate (ASN), and developing solution in turn. Each tooth was sectioned longitudinally to 1~2 mm slices and examined with stereo microscope and scanning electron microscope (SEM). The data were analyzed with SPSS 16.0 software package for Wilcoxon rank sum test.
  
  
  RESULTS
  There was varied microleakage at the margin. The difference was significant between group CHX and control group, between group MI and control group; However, there was no significant difference between group CHX and group MI.
  
  
  CONCLUSIONS
  MMPs inhibitor can reduce microleakage with wet bonding technique.
authors.affiliation: School of Stomatology, Jilin University. Changchun 130021, Jilin Province, China. E-mail:10124566@qq.com.
authors.forename: Cong
authors.initials: C
authors.lastname: Zhao
authors.forename: Shuang
authors.initials: S
authors.lastname: Chi
authors.forename: He
authors.initials: H
authors.lastname: Li
authors.forename: Tian-Bo
authors.initials: TB
authors.lastname: Li
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Zhang
mesh_terms: D001641:Bicuspid; D002710:Chlorhexidine; D003188:Composite Resins; D001840:Dental Bonding; D003731:Dental Caries; D003737:Dental Cavity Preparation / Q000379:methods; D003738:Dental Cements; D003763:Dental Leakage; D003793:Dental Restoration, Permanent; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006801:Humans; D061965:Matrix Metalloproteinase Inhibitors / Q000737:chemistry*; D020782:Matrix Metalloproteinases / Q000378:metabolism; D011897:Random Allocation; D019279:Resin Cements
chemical_list: D003188:Composite Resins; D003738:Dental Cements; D017220:Dentin-Bonding Agents; D061965:Matrix Metalloproteinase Inhibitors; D019279:Resin Cements; D020782:Matrix Metalloproteinases; D002710:Chlorhexidine