title: Identification of Stem-Like Cells in Atrial Myxoma by Markers CD44, CD19, and CD45.
abstract: Atrial myxoma is the most frequent tumor arising mainly in atrial septum and its origin remains uncertain. It has been reported that a subpopulation of stem-like cells are present in benign tumors and responsible for tumor initiation and maintenance. In this study, we investigated whether stem-like cells could contribute to the atrial cardiac myxoma. Immunohistology data confirmed that a population of cells bearing the surface markers CD19, CD45, and CD44 resided in a mucopolysaccharide-rich matrix of myxoma. Moreover, we isolated myxoma cells with phase-bright culture method and confirmed that myxoma derived cells express robust level of CD19, CD45, and CD44. Furthermore, the pluripotency of this population of cells also was validated by cardiomyocytes and smooth muscle cells differentiation in vitro. Our results indicate that primary cardiac myxoma may arise from mesenchymal stem cells with the ability to generate tumors with multilineage differentiation. In conclusion, this study for the first time verified that stem-like cells are present in atrial myxoma and this population of cells may have the capacity for myxoma initiation and progression.
authors.affiliation: Department of Cardiology, Rizhao Hospital of Traditional Chinese Medicine, Shandong 276800, China.
authors.forename: Xianghe
authors.initials: X
authors.lastname: Song
authors.affiliation: Nankai University School of Medicine, Tianjin 300071, China; Department of Cardiology, Tianjin Union Medical Center, Nankai University Affiliated Hospital, Tianjin 300121, China.
authors.forename: Danni
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Intensive Care Unit (ICU), People's Hospital of Rizhao, Shandong 276826, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Cui
authors.affiliation: Department of Radiation Oncology, Tianjin Union Medical Center, Tianjin 300121, China.
authors.forename: Manqian
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Department of Radiation Oncology, Tianjin Union Medical Center, Tianjin 300121, China.
authors.forename: Hui
authors.identifier: 0000-0003-0702-5406
authors.initials: H
authors.lastname: Wang
authors.affiliation: Nankai University School of Medicine, Tianjin 300071, China.
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.affiliation: Department of Cardiology, Tianjin Union Medical Center, Nankai University Affiliated Hospital, Tianjin 300121, China.
authors.forename: Xin
authors.identifier: 0000-0002-7193-5319
authors.initials: X
authors.lastname: Qi
authors.affiliation: Nankai University School of Medicine, Tianjin 300071, China; The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, The College of Life Science, Tianjin 300071, China.
authors.forename: Zongjin
authors.identifier: 0000-0002-4603-3743
authors.initials: Z
authors.lastname: Li