title: Trends in hepatocyte growth factor, insulin-like growth factor 1, thyroid-stimulating hormone, and leptin expression levels in uveal melanoma patient serum and tumor tissues: correlation to disease progression.
abstract: This exploratory study was carried out to determine the expression levels of hepatocyte growth factor (HGF), insulin-like growth factor 1, thyroid-stimulating hormone (TSH), and leptin in serum and tumor samples from patients with uveal melanoma and to investigate the potential association of these expression levels with disease progression and patient survival. Seventeen patients, including nine nonmetastatic and eight metastatic, were included in the study. Eighteen healthy individuals served as controls. The levels of these four proteins in serum and tissue samples were determined by enzyme-linked immunosorbent assays and immunohistochemical staining, respectively. Associations between protein levels and survival, disease progression, and other clinicopathological factors were analyzed statistically. Serum levels of HGF were significantly higher and TSH levels were lower in uveal melanoma patients than in healthy individuals, but the level of neither protein differed significantly between metastatic and nonmetastatic groups. Of the four proteins tested, only serum TSH was significantly associated with patient survival. No correlation was observed between the tissue and serum levels of each protein. The levels of HGF in serum may be markers of uveal melanoma development. The prognostic and predictive values of these potential markers need to be determined in a larger cohort.
authors.affiliation: Departments of aMelanoma Medical Oncology bOrbital Oncology and Ophthalmic Plastic Surgery cPlastic Surgery and Biostatistics dPathology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA eDepartment of Pathology, AC Camargo Cancer Center, São Paulo, Brazil.
authors.forename: Junna
authors.initials: J
authors.lastname: Oba
authors.forename: Bita
authors.initials: B
authors.lastname: Esmaeli
authors.forename: Julie A
authors.initials: JA
authors.lastname: Ellerhorst
authors.forename: Genevieve R
authors.initials: GR
authors.lastname: Lyons
authors.forename: Denái R
authors.initials: DR
authors.lastname: Milton
authors.forename: Wei-Lien
authors.initials: WL
authors.lastname: Wang
authors.forename: Mariana P
authors.initials: MP
authors.lastname: Macedo
authors.forename: Alexander J
authors.initials: AJ
authors.lastname: Lazar
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Grimm
authors.forename: Chandrani
authors.initials: C
authors.lastname: Chattopadhyay
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood; D018450:Disease Progression; D005260:Female; D017228:Hepatocyte Growth Factor / Q000097:blood*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D020738:Leptin / Q000097:blood*; D008297:Male; D008545:Melanoma / Q000097:blood* / Q000556:secondary; D008875:Middle Aged; D015996:Survival Rate; D013972:Thyrotropin / Q000097:blood*; D014604:Uveal Neoplasms / Q000097:blood* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; C583557:IGF1 protein, human; D020738:Leptin; D017228:Hepatocyte Growth Factor; D007334:Insulin-Like Growth Factor I; D013972:Thyrotropin