title: Lentivirus-mediated RNAi knockdown ofLMP2Ainhibits the growth of the Epstein-Barr-associated gastric carcinoma cell line GT38in vitro.
abstract: In this study, lentivirus-mediated RNA interference (RNAi) was applied to inhibit latent membrane protein 2A (LMP2A) gene expression, in order to explore the effects ofLMP2Asilencing on the growth of an Epstein-Barr virus-associated gastric carcinoma (EBVaGC) cell linein vitro. Lentivirus-mediated RNAi technology was employed to specifically knock down theLMP2Agene in the EBV-positive gastric carcinoma cell line GT38. After infection, reverse transcription-quantitative polymerase chain reaction, western blotting, flow cytometry and colony formation assays were conducted to evaluate the expression ofLMP2Aand the biological behavior of the GT38 cell linein vitro. The results showed that the expression of theLMP2Agene was clearly downregulated in the infected cells, which indicated that a highly efficient and stable lentivirus vector was successfully constructed. In the GT38 cells in which the expression ofLMP2Awas downregulated, the proliferation and colony formation of the cells was significantly inhibited. In addition, it was found that the cell cycle of the GT38 cells was arrested in the G0/G1 phase and the apoptosis rate was increased. These results indicate that lentivirus-mediated RNAi knockdown ofLMP2Ainhibits the growth of the EBVaGC cell line GT38in vitro, and suggests thatLMP2Ais a potential target for gene therapy in the treatment of EBVaGC.
authors.affiliation: Department of Gastroenterology, The Affiliated Jiangyin Hospital of Southeast University, Jiangyin, Jiangsu 214400, P.R. China; Department of Digestive Diseases, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.
authors.forename: Fangjun
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Digestive Diseases, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.
authors.forename: Weichang
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Gastroenterology, The Affiliated Jiangyin Hospital of Southeast University, Jiangyin, Jiangsu 214400, P.R. China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Liu
authors.affiliation: The Core Laboratory of Suzhou Cancer Center and Department of Radiotherapy, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215001, P.R. China.
authors.forename: Jundong
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Digestive Diseases, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.
authors.forename: Bingtuan
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology, The Affiliated Jiangyin Hospital of Southeast University, Jiangyin, Jiangsu 214400, P.R. China.
authors.forename: Wu
authors.initials: W
authors.lastname: Ye
authors.affiliation: Department of Gastroenterology, The Affiliated Jiangyin Hospital of Southeast University, Jiangyin, Jiangsu 214400, P.R. China.
authors.forename: Wenping
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, The Affiliated Jiangyin Hospital of Southeast University, Jiangyin, Jiangsu 214400, P.R. China.
authors.forename: Xiuyun
authors.initials: X
authors.lastname: Shen
keywords: RNA interference; gastric carcinoma; latent membrane protein 2A; lentivirus; proliferation