title: Purification and Characterization of 2S Albumin from Seeds of Wrightia tinctoria Exhibiting Antibacterial and DNase Activity.
abstract: 2S albumin is a low-molecular-weight seed storage protein belonging to the prolamin superfamily. In the present work a small 2S albumin (WTA) protein of ~16 kDa has been purified from the seeds of Wrightia tinctoria. The WTA is a heterodimer protein with a small subunit of ~5 kDa and a larger subunit of ~11 kDa bridged together through disulphide bonds. The protein exhibits deoxyribonucleases activity against closed circular pBR322 plasmid DNA and linear BL21 genomic DNA. The protein also showed antibacterial activity against Morexalla catarrhalis. CD studies indicate a high α-helical content in the protein. The conserved disulphide bonds in the protein suggest that the WTA is highly stable under high pH and temperature like other 2S albumin.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand-247667. India.
authors.forename: Anchal
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand-247667. India.
authors.forename: Pramod
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand-247667. India.
authors.forename: Pooja
authors.initials: P
authors.lastname: Kesari
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand-247667. India.
authors.lastname: Neetu
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand-247667. India.
authors.forename: Madhusudhanarao
authors.initials: M
authors.lastname: Katiki
authors.affiliation: Genetics and Plant Molecular Biology, Council of Scientific and Industrial Research (CSIR)-National Botanical Research Institute, Lucknow. India.
authors.forename: Manisha
authors.initials: M
authors.lastname: Mishra
authors.affiliation: Genetics and Plant Molecular Biology, Council of Scientific and Industrial Research (CSIR)-National Botanical Research Institute, Lucknow. India.
authors.forename: Pradhyumna K
authors.initials: PK
authors.lastname: Singh
authors.affiliation: Department of Civil Engineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand-247667. India.
authors.forename: Bhola R
authors.initials: BR
authors.lastname: Gurjar
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand-247667. India.
authors.forename: Ashwani K
authors.initials: AK
authors.lastname: Sharma
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand-247667. India.
authors.forename: Shailly
authors.initials: S
authors.lastname: Tomar
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand-247667. India.
authors.forename: Pravindra
authors.initials: P
authors.lastname: Kumar
mesh_terms: D055516:2S Albumins, Plant / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D029062:Apocynaceae / Q000737:chemistry*; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D003851:Deoxyribonucleases / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018985:Epitopes, B-Lymphocyte; D001936:Moraxella catarrhalis / Q000187:drug effects; D012639:Seeds / Q000737:chemistry
chemical_list: D055516:2S Albumins, Plant; D000900:Anti-Bacterial Agents; D018985:Epitopes, B-Lymphocyte; D004247:DNA; D003851:Deoxyribonucleases
keywords: 2S Albumin; IgE binding
  allergen; Wrightia tinctoria; b-cell epitope; deoxyribonucleases activity; seed storage protein