title: Mrpl10 and Tbp Are Suitable Reference Genes for Peripheral Nerve Crush Injury.
abstract: Peripheral nerve injury triggers the dysregulation of a large number of genes at multiple sites, including neurons, peripheral nerve stump, and the target organ. Housekeeping genes were frequently used as reference genes to normalize the expression values of target genes. Suitable selection of housekeeping genes that are stably expressed after nerve injury minimizes bias elicited by reference genes and thus helps to better and more sensitively reflect gene expression changes. However, many housekeeping genes have been used as reference genes without testing the expression patterns of themselves. In the current study, we calculated the expression stability of nine commonly used housekeeping genes, such as18S(18S ribosomal RNA),Actb(β-actin),CypA(cyclophilin A),Gapdh(glyceraldehydes-3-phosphate dehydrogenase),Hprt(hypoxanthine guanine phosphoribosyl transferase),Pgk1(phosphoglycerate kinase 1),Tbp(TATA box binding protein),Ubc(ubiquitin C),YwhaZ(tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation), and four newly identified housekeeping genes, includingAnkrd27(Ankyrin repeat domain 27),Mrpl10(mitochondrial ribosomal protein L10),Rictor(rapamycin-insensitive companion of mTOR, Complex 2), andUbxn 11(UBX domain protein 11), in both distal sciatic nerve samples and dorsal root ganglion (DRG) samples after sciatic nerve injury. Our results suggested that following peripheral nerve injury,Mrpl10andTbpmight be used as suitable reference genes for sciatic nerve stump and DRGs, respectively.
authors.affiliation: School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China. wyx1984@ntu.edu.cn.|Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China. wyx1984@ntu.edu.cn.
authors.forename: Yaxian
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Radiotherapy and Oncology, The Affiliated Hospital of Nantong University, Nantong 226001, China. shanqianiqan1986@126.com.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Shan
authors.affiliation: Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China. 18705592415@163.com.
authors.forename: Yali
authors.initials: Y
authors.lastname: Meng
authors.affiliation: Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China. kaixinge@126.com.
authors.forename: Jiacheng
authors.initials: J
authors.lastname: Pan
authors.affiliation: Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China. syi@ntu.edu.cn.
authors.forename: Sheng
authors.initials: S
authors.lastname: Yi
mesh_terms: D000818:Animals; D000071576:Crush Injuries / Q000235:genetics*; D005260:Female; D005727:Ganglia, Spinal / Q000378:metabolism; D005786:Gene Expression Regulation; D020043:Genes, Essential; D007150:Immunohistochemistry; D009409:Nerve Crush; D059348:Peripheral Nerve Injuries / Q000235:genetics*; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D012269:Ribosomal Proteins / Q000235:genetics* / Q000378:metabolism; D012584:Sciatic Nerve / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D012984:Software; D035181:TATA-Box Binding Protein / Q000235:genetics* / Q000378:metabolism
chemical_list: D012269:Ribosomal Proteins; D035181:TATA-Box Binding Protein
keywords: NormFinder; distal nerve stump; dorsal root ganglion; geNorm; housekeeping gene; rat peripheral nerve injury