title: Downregulated miR-29a/b/c during Contact Inhibition Stage Promote 3T3-L1 Adipogenesis by Targeting DNMT3A.
abstract: Differentiation of 3T3-L1 cells into adipocytes involves a highly-orchestrated series of events including contact inhibition (CI), clonal expansion, growth arrest, and terminal differentiation. Recent study demonstrated that 3T3-L1 preadipocytes will not be differentiated into mature adipocytes without CI stage, which indicated that CI stage plays an important role during 3T3-L1 adipogenesis. However, the molecular mechanism is not yet fully understood. In the present study, we found that the expression level of miR-29a/b/c was decreased and the expression of DNMT3A was up-regulated during CI stage, respectively. Furthermore, overexpression of miR-29a/b/c during CI stage inhibits adipogenesis significantly but not at other stages. In addition, miR-29a/b/c repressed DNMT3A expression by directly targeting its 3' untranslated region (3' UTR). Our data reveal a novel mechanism of miR-29a/b/c in the regulation of adipogenesis.
authors.affiliation: Henan Provincial Luoyang Orthopedic-Traumatological Hospital (Henan Provincial Orthopedic Hospital), Luoyang, Henan, China.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: CAS-MPG Partner Institute for Computational Biology, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Guangyong
authors.initials: G
authors.lastname: Zheng
authors.affiliation: Henan Provincial Luoyang Orthopedic-Traumatological Hospital (Henan Provincial Orthopedic Hospital), Luoyang, Henan, China.
authors.forename: Huichao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Henan Provincial Luoyang Orthopedic-Traumatological Hospital (Henan Provincial Orthopedic Hospital), Luoyang, Henan, China.
authors.forename: Yudong
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Henan Provincial Luoyang Orthopedic-Traumatological Hospital (Henan Provincial Orthopedic Hospital), Luoyang, Henan, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Henan Provincial Luoyang Orthopedic-Traumatological Hospital (Henan Provincial Orthopedic Hospital), Luoyang, Henan, China.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Henan Provincial Luoyang Orthopedic-Traumatological Hospital (Henan Provincial Orthopedic Hospital), Luoyang, Henan, China.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Li
authors.affiliation: Henan Provincial Luoyang Orthopedic-Traumatological Hospital (Henan Provincial Orthopedic Hospital), Luoyang, Henan, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Henan Provincial Luoyang Orthopedic-Traumatological Hospital (Henan Provincial Orthopedic Hospital), Luoyang, Henan, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Henan Provincial Luoyang Orthopedic-Traumatological Hospital (Henan Provincial Orthopedic Hospital), Luoyang, Henan, China.
authors.forename: Youwen
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China.
authors.forename: Sanhong
authors.identifier: http://orcid.org/0000-0002-8695-0343
authors.initials: S
authors.lastname: Liu
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000235:genetics*; D000818:Animals; D002454:Cell Differentiation; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000378:metabolism*; D000090205:DNA Methyltransferase 3A; D015536:Down-Regulation; D020869:Gene Expression Profiling; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D015398:Signal Transduction
chemical_list: C000717967:DNMT3A protein, human; C000715535:Dnmt3a protein, mouse; C547014:MIRN29 microRNA, mouse; D035683:MicroRNAs; D004248:DNA (Cytosine-5-)-Methyltransferases; D000090205:DNA Methyltransferase 3A