title: Insights into the Planktothrix genus: Genomic and metabolic comparison of benthic and planktic strains.
abstract: Planktothrix is a dominant cyanobacterial genus forming toxic blooms in temperate freshwater ecosystems. We sequenced the genome of planktic and non planktic Planktothrix strains to better represent this genus diversity and life style at the genomic level. Benthic and biphasic strains are rooting the Planktothrix phylogenetic tree and widely expand the pangenome of this genus. We further investigated in silico the genetic potential dedicated to gas vesicles production, nitrogen fixation as well as natural product synthesis and conducted complementary experimental tests by cell culture, microscopy and mass spectrometry. Significant differences for the investigated features could be evidenced between strains of different life styles. The benthic Planktothrix strains showed unexpected characteristics such as buoyancy, nitrogen fixation capacity and unique natural product features. In comparison with Microcystis, another dominant toxic bloom-forming genus in freshwater ecosystem, different evolutionary strategies were highlighted notably as Planktothrix exhibits an overall greater genetic diversity but a smaller genomic plasticity than Microcystis. Our results are shedding light on Planktothrix evolution, phylogeny and physiology in the frame of their diverse life styles.
authors.affiliation: Institut Pasteur, Collection des Cyanobactéries, 28 rue du Dr Roux, 75724 Paris Cedex 05, France.|UMR UPMC 113, CNRS 7618, IRD 242, INRA 1392, PARIS 7 113, UPEC, IEES Paris, 4 Place Jussieu, 75005, Paris, France.|Université Pierre et Marie Curie (UPMC), 4 Place Jussieu, 75005, Paris, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Pancrace
authors.affiliation: UMR UPMC 113, CNRS 7618, IRD 242, INRA 1392, PARIS 7 113, UPEC, IEES Paris, 4 Place Jussieu, 75005, Paris, France.
authors.forename: Marie-Anne
authors.initials: MA
authors.lastname: Barny
authors.affiliation: Institute of Microbiology, Eigenössische Technische Hochschule (ETH) Zurich, Vladimir-Prelog-Weg 4, 8093 Zurich, Switzerland.
authors.forename: Reiko
authors.initials: R
authors.lastname: Ueoka
authors.affiliation: Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Genoscope &CNRS, UMR 8030, Laboratoire d'Analyse Bioinformatique en Génomique et Métabolisme, 2, rue Gaston Crémieux, CP 5706, 91057 EVRY cedex, France.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Calteau
authors.affiliation: Institut Pasteur, Collection des Cyanobactéries, 28 rue du Dr Roux, 75724 Paris Cedex 05, France.
authors.forename: Thibault
authors.initials: T
authors.lastname: Scalvenzi
authors.affiliation: UMR UPMC 113, CNRS 7618, IRD 242, INRA 1392, PARIS 7 113, UPEC, IEES Paris, 4 Place Jussieu, 75005, Paris, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Pédron
authors.affiliation: Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Genoscope, Laboratoire de Biologie Moléculaire pour l'étude des Génomes, 2, rue Gaston Crémieux, CP 5706, 91057 EVRY cedex, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Barbe
authors.affiliation: Institute of Microbiology, Eigenössische Technische Hochschule (ETH) Zurich, Vladimir-Prelog-Weg 4, 8093 Zurich, Switzerland.
authors.forename: Joern
authors.initials: J
authors.lastname: Piel
authors.affiliation: UMR UPMC 113, CNRS 7618, IRD 242, INRA 1392, PARIS 7 113, UPEC, IEES Paris, 4 Place Jussieu, 75005, Paris, France.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Humbert
authors.affiliation: Institut Pasteur, Collection des Cyanobactéries, 28 rue du Dr Roux, 75724 Paris Cedex 05, France.
authors.forename: Muriel
authors.initials: M
authors.lastname: Gugger
mesh_terms: D014644:Genetic Variation; D016678:Genome; D016680:Genome, Bacterial; D023281:Genomics; D054300:Oscillatoria / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny