title: In Vivo Selective Capture and Rapid Identification of Luteolin and Its Metabolites in Rat Livers by Molecularly Imprinted Solid-Phase Microextraction.
abstract: A method based on molecularly imprinted solid-phase microextraction (MIP-SPME) coupled with liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (QTOF-MS/MS) was developed for the detection of luteolin and its metabolites in vivo. The MIP-SPME fibers were first fabricated by dopamine and silane, and then luteolin MIPs-coated fibers were successfully prepared using luteolin, acrylamide (AM), and ethylene glycol dimethacrylate (EGDMA) as the template, functional monomer and cross-linker, respectively. The characterizations of polymers were analyzed by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and the Brunauer-Emmett-Teller method (BET). The properties involving adsorption and selective experiments were evaluated, and these results revealed that MIP fibers presented high adsorption capacity and selectivity to luteolin. Furthermore, the developed MIP-SPME coupled with the LC-QTOF-MS/MS method was adopted to capture and identify luteolin and its metabolites in rat livers in vivo, and eventually, apigenin, chrysoeriol, and diosmetin were rapidly identified as metabolites.
authors.affiliation: School of Pharmaceutical Sciences, Chongqing University , Chongqing 400030, China.|Department of Pharmaceutical Sciences, School of Pharmacy, Southwest Medical University , Luzhou, Sichuan 646000, China.|School of Chemistry and Chemical Engineering, Chongqing University , Chongqing 400030, China.
authors.forename: Die
authors.initials: D
authors.lastname: Gao
authors.affiliation: School of Pharmaceutical Sciences, Chongqing University , Chongqing 400030, China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University , Chongqing 400030, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University , Chongqing 400030, China.
authors.forename: Feng-Qing
authors.identifier: http://orcid.org/0000-0002-9300-5710
authors.initials: FQ
authors.lastname: Yang
authors.affiliation: School of Pharmaceutical Sciences, Chongqing University , Chongqing 400030, China.|School of Chemistry and Chemical Engineering, Chongqing University , Chongqing 400030, China.
authors.forename: Zhi-Ning
authors.identifier: http://orcid.org/0000-0002-2064-5880
authors.initials: ZN
authors.lastname: Xia
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University , Chongqing 400030, China.
authors.forename: Qi-Hui
authors.initials: QH
authors.lastname: Zhang
authors.affiliation: Tang Center for Herbal Medicine Research and Department of Anesthesia & Critical Care, University of Chicago , Chicago, Illinois 60637, United States.
authors.forename: Chun-Su
authors.initials: CS
authors.lastname: Yuan
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008099:Liver / Q000737:chemistry* / Q000378:metabolism; D047311:Luteolin / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting; D017207:Rats, Sprague-Dawley; D052617:Solid Phase Microextraction / Q000295:instrumentation / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D047311:Luteolin
keywords: HPLC-MS/MS; in vivo sampling; luteolin; metabolites; molecularly imprinted polymer; solid phase microextraction