title: A combined biomarker and clinical panel for chronic graft versus host disease diagnosis.
abstract: Whilst many chronic graft versus host disease (cGVHD) biomarkers have been previously reported, few have been verified in an independent cGVHD cohort. We aimed to verify the diagnostic accuracy of previously reported markers of cGVHD in a multi-centre Chronic GVHD Consortium. A total of 42 RNA and 18 protein candidate biomarkers were assessed amongst 59 cGVHD cases and 33 matched non-GVHD controls. Total RNA was isolated from PBMC, and RNA markers were quantified using PCR. Serum protein markers were quantified using ELISA. A combined 3 RNA biomarker (IRS2, PLEKHF1 and IL1R2) and 2 clinical variables (recipient CMV serostatus and conditioning regimen intensity) panel accurately (AUC 0.81) segregated cGVHD cases from controls. Other studied RNA and protein markers were not confirmed as accurate cGVHD diagnostic biomarkers. The studied markers failed to segregate higher risk cGVHD (per overall NIH 0-3 score, and overlap versus classic cGVHD status). These data support the need for multiple independent verification studies for the ultimate clinical application of cGVHD diagnostic biomarkers.
authors.affiliation: Department of Blood and Marrow Transplantation H. Lee Moffitt Cancer Center and Research Institute Tampa FL USA.
authors.forename: Joseph
authors.initials: J
authors.lastname: Pidala
authors.affiliation: Department of Surgery University of California San Francisco San Francisco CA USA.
authors.forename: Tara K
authors.initials: TK
authors.lastname: Sigdel
authors.affiliation: Department of Surgery University of California San Francisco San Francisco CA USA.
authors.forename: Anyou
authors.initials: A
authors.lastname: Wang
authors.affiliation: Department of Surgery University of California San Francisco San Francisco CA USA.
authors.forename: Sue
authors.initials: S
authors.lastname: Hsieh
authors.affiliation: Clinical Research Division Fred Hutchinson Cancer Research Center Seattle WA USA.
authors.forename: Yoshi
authors.initials: Y
authors.lastname: Inamoto
authors.affiliation: Clinical Research Division Fred Hutchinson Cancer Research Center Seattle WA USA.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Martin
authors.affiliation: Clinical Research Division Fred Hutchinson Cancer Research Center Seattle WA USA.
authors.forename: Mary Ed
authors.initials: ME
authors.lastname: Flowers
authors.affiliation: Clinical Research Division Fred Hutchinson Cancer Research Center Seattle WA USA.
authors.forename: John A
authors.initials: JA
authors.lastname: Hansen
authors.affiliation: Clinical Research Division Fred Hutchinson Cancer Research Center Seattle WA USA.
authors.forename: Stephanie J
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Surgery University of California San Francisco San Francisco CA USA.
authors.forename: Minnie M
authors.initials: MM
authors.lastname: Sarwal
keywords: chronic graft versus host disease; diagnostic biomarkers