title: Characterisation of the regulatory function of the H-NS protein in the benthic bacterium Shewanella piezotolerans WP3 under cold conditions.
abstract: The histone-like nucleoid structuring (H-NS) protein is conserved in Gram-negative bacteria and plays important roles as a multifunctional bacterial modulator. However, its roles in deep-sea microorganisms warrant investigation. In this study, the relationship between temperature and the regulatory function of H-NS was characterised in the benthic bacterium Shewanella piezotolerans WP3. Comparative microarray analysis of the hns knockout mutant and wild-type strain identified a total of 264 differentially expressed genes at 4°C, which is significantly less than that at 20°C. Notably, H-NS was proved to be involved in the cold-mediated induction of lateral flagellar gene transcription. We further showed that the binding affinity of H-NS is higher at 4°C than 20°C, thus explaining the significant difference in the expression pattern at different temperatures.
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 200240 Shanghai, PR China.
authors.forename: Huahua
authors.initials: H
authors.lastname: Jian
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 200240 Shanghai, PR China.
authors.forename: Guanpeng
authors.initials: G
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 200240 Shanghai, PR China.
authors.forename: Fengping
authors.initials: F
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 200240 Shanghai, PR China.|State Key Laboratory of Ocean Engineering, School of Naval Architecture, Ocean and Civil Engineering, Shanghai Jiao Tong University, 200240 Shanghai, PR China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Xiao
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D003080:Cold Temperature; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005407:Flagella / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D055786:Gene Knockout Techniques; D019015:Geologic Sediments / Q000382:microbiology*; D046228:Microarray Analysis; D009154:Mutation; D012623:Seawater / Q000382:microbiology*; D020592:Shewanella / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C031908:H-NS protein, bacteria
keywords: H-NS; deep sea; lateral flagella; low temperature; microarray