title: Silver nanoparticles decorated lipase-sensitive polyurethane micelles for on-demand release of silver nanoparticles.
abstract: In order to improve the antibacterial activities while decrease the cytotoxity of silver nanoparticles, we prepared a novel nanocomposites composed of silver nanoparticles decorated lipase-sensitive polyurethane micelles (PUM-Ag) with MPEG brush on the surface. The nanocomposite was characterized by UV-vis, TEM and DLS. UV-vis and TEM demonstrated the formation of silver nanoparticles on PU micelles and the nanoassembly remained intact without the presence of lipase. The silver nanoparticles were protected by the polymer matrix and PEG brush which show good cytocompatibility to HUVEC cells and low hemolysis. Moreover, at the presence of lipase, the polymer matrix of nanocomposites is subject to degradation and the small silver nanoparticles were released as is shown by DLS and TEM. The MIC and MBC studies showed an enhanced toxicity of the nanocomposites to both gram negative and gram positive bacteria, i.e. E. coli and S. aureus, as the result of the degradation of polymer matrix by bacterial lipase. Therefore, the nanocomposites are biocompatible to mammalian cells cells which can also lead to activated smaller silver nanoparticles release at the presence of bacteria and subsequently enhanced inhibition of bacteria growth. The satisfactory selectivity for bacteria compared to HUVEC and RBCs make PUM-Ag a promising antibacterial nanomedicine in biomedical field.
authors.affiliation: College of Chemistry and Environmental Protection Engineering, Southwest University for Nationalities, 610041 Chengdu, China.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Su
authors.affiliation: College of Chemistry and Environmental Protection Engineering, Southwest University for Nationalities, 610041 Chengdu, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhao
authors.affiliation: College of Chemistry and Environmental Protection Engineering, Southwest University for Nationalities, 610041 Chengdu, China.
authors.forename: Fancui
authors.initials: F
authors.lastname: Meng
authors.affiliation: College of Chemistry and Environmental Protection Engineering, Southwest University for Nationalities, 610041 Chengdu, China.
authors.forename: Quanxin
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Chemistry and Environmental Protection Engineering, Southwest University for Nationalities, 610041 Chengdu, China.
authors.forename: Yongchao
authors.initials: Y
authors.lastname: Yao
authors.affiliation: College of Chemistry and Environmental Protection Engineering, Southwest University for Nationalities, 610041 Chengdu, China. Electronic address: luojb1971@163.com.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Luo
mesh_terms: D004926:Escherichia coli / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008049:Lipase / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000009:adverse effects / Q000737:chemistry*; D008823:Micelles; D053761:Nanocomposites / Q000737:chemistry; D050997:Nanomedicine; D011108:Polymers / Q000009:adverse effects / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D011140:Polyurethanes / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D008823:Micelles; D011108:Polymers; D011140:Polyurethanes; D012834:Silver; D008049:Lipase
keywords: Antibacterial; Biocompatible; Lipase sensitive; Polyurethane micelles; Silver nanoparticles