title: Nano-precipitated curcumin loaded particles: effect of carrier size and drug complexation with (2-hydroxypropyl)-β-cyclodextrin on their biological performances.
abstract: In this work, curcumin (CURC)-encapsulating nanoparticles (NPs), made up of an amphiphilic blend of poloxamers and PLGA (PPC NPs) at different polymer concentrations, were prepared by nanoprecipitation. CURC was preliminarily complexed with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) to improve its loading efficiency. The formation of host-guest complexes of CURC with HPβCD (CD-CURC) was confirmed by means of1HNMR studies and differential scanning calorimetry (DSC). Nanoprecipitation allowed to obtain NPs with a small size (90-120nm depending on the polymer concentration), a narrow size distribution and stable in water for 30days at 4°C and in RPMI-1640 cell culture medium up to 72h at 37°C. The in vitro release of CD-CURC, sustained up to 5days, was governed mainly by a diffusive mechanism. It was also found that the produced NPs were efficiently internalized by mesothelioma cells (MSTO-211H) in the cytoplasmic space, at an extent strongly dependent on NP size and polydispesity index, therefore pointing at the importance of NP preparation method in improving their uptake.
authors.affiliation: Dipartimento di Scienze del Farmaco e Prodotti per la Salute, Università di Messina, Piazza Pugliatti 1, Messina, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Serri
authors.affiliation: Istituto di Bioscienze e Biorisorse - CNR, Via Pietro Castellino 111, Napoli, Italy; Istituto di Genetica e Biofisica "ABT" - CNR, Via Pietro Castellino 111, Napoli, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Argirò
authors.affiliation: Istituto di Cristallografia - CNR, Via G. Amendola 122/O, Bari, Italy.
authors.forename: Linda
authors.initials: L
authors.lastname: Piras
authors.affiliation: Istituto di Genetica e Biofisica "ABT" - CNR, Via Pietro Castellino 111, Napoli, Italy; Consorzio Interuniversitario INBB: Laboratorio Nazionale Interferenti Endocrini, Via Pietro Castellino 111, Napoli, Italy.
authors.forename: Damiano Gustavo
authors.initials: DG
authors.lastname: Mita
authors.affiliation: Dipartimento di Scienze del Farmaco e Prodotti per la Salute, Università di Messina, Piazza Pugliatti 1, Messina, Italy.
authors.forename: Antonina
authors.initials: A
authors.lastname: Saija
authors.affiliation: Istituto di Genetica e Biofisica "ABT" - CNR, Via Pietro Castellino 111, Napoli, Italy; Consorzio Interuniversitario INBB: Laboratorio Nazionale Interferenti Endocrini, Via Pietro Castellino 111, Napoli, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Mita
authors.affiliation: Istituto di Bioscienze e Biorisorse - CNR, Via Pietro Castellino 111, Napoli, Italy; Istituto di Genetica e Biofisica "ABT" - CNR, Via Pietro Castellino 111, Napoli, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Forte
authors.affiliation: Dipartimento di Farmacia, Università di Napoli Federico II, Via D. Montesano 49, Napoli, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Giarra
authors.affiliation: Consorzio Interuniversitario INBB: Laboratorio Nazionale Interferenti Endocrini, Via Pietro Castellino 111, Napoli, Italy; Dipartimento di Farmacia, Università di Napoli Federico II, Via D. Montesano 49, Napoli, Italy; Interdisciplinary Research Centre on Biomaterials - CRIB - Università di Napoli Federico II, P.le Tecchio, 80, Napoli, Italy. Electronic address: mabiondi@unina.it.
authors.forename: Marco
authors.initials: M
authors.lastname: Biondi
authors.affiliation: Istituto di Bioscienze e Biorisorse - CNR, Via Pietro Castellino 111, Napoli, Italy; Consorzio Interuniversitario INBB: Laboratorio Nazionale Interferenti Endocrini, Via Pietro Castellino 111, Napoli, Italy. Electronic address: stefania.crispi@ibbr.cnr.it.
authors.forename: Stefania
authors.initials: S
authors.lastname: Crispi
authors.affiliation: Consorzio Interuniversitario INBB: Laboratorio Nazionale Interferenti Endocrini, Via Pietro Castellino 111, Napoli, Italy; Dipartimento di Farmacia, Università di Napoli Federico II, Via D. Montesano 49, Napoli, Italy; Interdisciplinary Research Centre on Biomaterials - CRIB - Università di Napoli Federico II, P.le Tecchio, 80, Napoli, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Mayol
mesh_terms: D000073738:2-Hydroxypropyl-beta-cyclodextrin; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D011232:Chemical Precipitation; D003474:Curcumin / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D004355:Drug Stability; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism; D010316:Particle Size; D020442:Poloxamer / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D047392:beta-Cyclodextrins; D020442:Poloxamer; D000073738:2-Hydroxypropyl-beta-cyclodextrin; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D003474:Curcumin
keywords: Curcumin; Cyclodextrin; Mesothelioma; Nanoparticles; PLGA; Poloxamers