title: Simultaneous determination of rifabutin and human serum albumin in pharmaceutical formulations by capillary electrophoresis.
abstract: Capillary zone electrophoresis (CZE) was used for determination of rifabutin (RFB), an anti-tuberculosis antibiotic drug, in various pharmaceutical formulations. Apart from that, simultaneous determination of RFB and human serum albumin (HSA) was performed. Electrophoretic behaviour of RFB was examined at various pH levels. CE conditions: a quartz capillary tube (internal diameter 75mm, effective length 50cm, total length 60cm), the capillary temperature was 25°С, the voltage applied to the capillary tube was +20kV, the UV detection wavelength was 214nm, hydrodynamic injection of the sample was performed at 30mbar for 5s, tetraborate buffer solution (0.01М, рН9.2). The obtained results are characterized by high efficiency (number of theoretical plates up to 260,000) and sufficient sensitivity (LOQ starting from 0.02μg/ml for RFB). The obtained data are in good accord with both HPLC results (for RFB) and spectrophotometry (for HSA).
authors.affiliation: Drugs Technology LLC, Khimki, Moscow Region, Russia; D. Mendeleev University of Chemical Technology of Russia, Moscow, Russia. Electronic address: uve2007@yandex.ru.
authors.forename: Yu
authors.initials: Y
authors.lastname: Ermolenko
authors.affiliation: D. Mendeleev University of Chemical Technology of Russia, Moscow, Russia.
authors.forename: A
authors.initials: A
authors.lastname: Anshakova
authors.affiliation: Drugs Technology LLC, Khimki, Moscow Region, Russia.
authors.forename: N
authors.initials: N
authors.lastname: Osipova
authors.affiliation: Saint Petersburg State University, Institute of Chemistry, St. Petersburg, Russia.
authors.forename: M
authors.initials: M
authors.lastname: Kamentsev
authors.affiliation: Drugs Technology LLC, Khimki, Moscow Region, Russia.
authors.forename: O
authors.initials: O
authors.lastname: Maksimenko
authors.affiliation: Drugs Technology LLC, Khimki, Moscow Region, Russia.
authors.forename: V
authors.initials: V
authors.lastname: Balabanyan
authors.affiliation: Drugs Technology LLC, Khimki, Moscow Region, Russia.
authors.forename: S
authors.initials: S
authors.lastname: Gelperina
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis / Q000737:chemistry; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D004339:Drug Compounding; D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans; D017828:Rifabutin / Q000032:analysis* / Q000737:chemistry; D000075462:Serum Albumin, Human / Q000032:analysis* / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D017828:Rifabutin; D000075462:Serum Albumin, Human
keywords: Capillary electrophoresis (CE); Human serum albumin (HSA); Rifabutin (RFB)