title: Spirosoma knui sp. nov., a radiation-resistant bacterium isolated from the Han River.
abstract: A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, aerobic bacterium, designated 15J8-12T, was isolated from a water sample after exposure to 3 kGy of gamma radiation. The strain showed resistance to gamma radiation with a dose required to reduce the bacterial population 10 fold (D10) value of 4.7 kGy. The results of comparative 16S rRNA gene sequence analysis indicated that strain 15J8-12T represented a member of the family Cytophagaceae, phylum Bacteroidetes, and was most closely related to 'Spirosomafluminis' 15J17 (97.92 %) and Spirosoma arcticum R2-35T (92.22 %). The G+C content of the genomic DNA of 15J8-12T was 51.3 mol%. The detection of menaquinone MK-7 as the predominant respiratory quinone, a fatty acid profile with summed feature 3 (C16 : 1ω7c/C16 : 1ω6c; 40.5 %), C16 : 1ω5c (35.3 %), C15 : 0 iso (6.9 %) and C16 : 0 (6.8 %) as the major components and phosphatidylethanolamine as the major polar lipid also supported the affiliation of 15J8-12T with the genus Spirosoma. The DNA-DNA relatedness between 15J8-12T and 'Spirosoma fluminis' 15J17 was 27.8 %. On the basis of its phenotypic and genotypic properties, together with its phylogenetic distinctiveness, 15J8-12T should be considered to be a representative of a novel species of the genus Spirosoma, for which the name Spirosoma knui sp. nov. is proposed. The type strain is 15J8-12T (=KCTC 52510T=JCM 31407T).
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea.
authors.forename: Jae-Jin
authors.initials: JJ
authors.lastname: Lee
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea.
authors.forename: Yeon-Hee
authors.initials: YH
authors.lastname: Lee
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea.
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Park
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea.
authors.forename: Seung-Yeol
authors.initials: SY
authors.lastname: Lee
authors.affiliation: School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea.
authors.forename: Byung-Oh
authors.initials: BO
authors.lastname: Kim
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea.
authors.forename: Leonid N
authors.initials: LN
authors.lastname: Ten
authors.affiliation: Department of Bio and Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 01797, Republic of Korea.
authors.forename: Myung Kyum
authors.initials: MK
authors.lastname: Kim
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea.|Institute of Plant Medicine, Kyungpook National University, Daegu 41566, Republic of Korea.
authors.forename: Hee-Young
authors.initials: HY
authors.lastname: Jung
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000528:radiation effects*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005720:Gamma Rays; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C062629:menaquinone 7