title: Budding Yeast: An Ideal Backdrop forIn vivoLipid Biochemistry.
abstract: Biological membranes are non-covalent assembly of lipids and proteins. Lipids play critical role in determining membrane physical properties and regulate the function of membrane associated proteins. Budding yeastSaccharomyces cerevisiaeoffers an exceptional advantage to understand the lipid-protein interactions since lipid metabolism and homeostasis are relatively simple and well characterized as compared to other eukaryotes. In addition, a vast array of genetic and cell biological tools are available to determine and understand the role of a particular lipid in various lipid metabolic disorders. Budding yeast has been instrumental in delineating mechanisms related to lipid metabolism, trafficking and their localization in different subcellular compartments at various cell cycle stages. Further, availability of tools and enormous potential for the development of useful reagents and novel technologies to localize a particular lipid in different subcellular compartments in yeast makes it a formidable system to carry out lipid biology. Taken together, yeast provides an outstanding backdrop to characterize lipid metabolic changes under various physiological conditions.
authors.affiliation: Center for Cell Dynamics, Department of Cell Biology, Johns Hopkins University School of MedicineBaltimore, MD, USA; Department of Chemical and Biomolecular Engineering, Whiting School of Engineering, Johns Hopkins UniversityBaltimore, MD, USA.
authors.forename: Pushpendra
authors.initials: P
authors.lastname: Singh
keywords: budding yeast; ergosterol; lipid sensors; lipid-protein interactions; sphingolipid