title: Production of Rare Phyto-Ceramides from Abundant Food Plant Residues.
abstract: Ceramides (Cers) are major components of the outermost layer of the skin, the stratum corneum, and play a crucial role in permeability barrier functions. Alterations in Cer composition causing skin diseases are compensated with semisynthetic skin-identical Cers. Plants constitute new resources for Cer production as they contain glucosylceramides (GluCers) as major components. GluCers were purified from industrial waste plant materials, apple pomace (Malus domestica), wheat germs (Triticum sp.), and coffee grounds (Coffea sp.), with GluCer contents of 28.9 mg, 33.7 mg, and 4.4 mg per 100 g of plant material. Forty-five species of GluCers (1-45) were identified with different sphingoid bases, saturated or monounsaturated α-hydroxy fatty acids (C15-28), and β-glucose as polar headgroup. Three main GluCers were hydrolyzed by a recombinant human glucocerebrosidase to produce phyto-Cers (46-48). These studies showed that rare and expensive phyto-Cers can be obtained from industrial food plant residues.
authors.affiliation: Department of Pharmaceutical Biology and Pharmacology, Institute of Pharmacy, Faculty of Natural Sciences I, Martin Luther University Halle-Wittenberg , Hoher Weg 8, D-06120 Halle (Saale), Germany.
authors.forename: Mathias
authors.initials: M
authors.lastname: Reisberg
authors.affiliation: Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry , Weinberg 3, D-06120 Halle (Saale), Germany.
authors.forename: Norbert
authors.initials: N
authors.lastname: Arnold
authors.affiliation: Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry , Weinberg 3, D-06120 Halle (Saale), Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Porzel
authors.affiliation: Department of Pharmaceutical Technology and Biopharmaceutics, Institute of Pharmacy, Faculty of Natural Sciences I, Martin Luther University Halle-Wittenberg , Wolfgang-Langenbeck-Str. 4, D-06120 Halle (Saale), Germany.
authors.forename: Reinhard H H
authors.initials: RH
authors.lastname: Neubert
authors.affiliation: Department of Pharmaceutical Biology and Pharmacology, Institute of Pharmacy, Faculty of Natural Sciences I, Martin Luther University Halle-Wittenberg , Hoher Weg 8, D-06120 Halle (Saale), Germany.
authors.forename: Birgit
authors.identifier: http://orcid.org/0000-0002-0615-5674
authors.initials: B
authors.lastname: Dräger
mesh_terms: D002518:Ceramides / Q000737:chemistry* / Q000302:isolation & purification; D040503:Coffea / Q000737:chemistry*; D005504:Food Analysis; D027845:Malus / Q000737:chemistry*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D014908:Triticum / Q000737:chemistry*; D014866:Waste Products / Q000032:analysis*
chemical_list: D002518:Ceramides; D010936:Plant Extracts; D014866:Waste Products
keywords: Coffea sp.; Malus domestica; Triticum sp.; apple pomace; ceramide; coffee grounds; glucocerebrosidase; glucosylceramide; wheat germs