title: Arbuscular mycorrhizal fungi inoculation mediated changes in rhizosphere bacterial community structure while promoting revegetation in a semiarid ecosystem.
abstract: The main goal of this study was to assess the effect of the inoculation of four autochthonous shrub species with the arbuscular mycorrhizal (AM) fungus Rhizophagus intraradices on the rhizosphere bacterial community and to ascertain whether such an effect is dependent on the host plant species. Additionally, analysis of rhizosphere soil chemical and biochemical properties was performed to find relationships between them and the rhizosphere bacterial communities. Non-metric multidimensional scaling analysis and subsequent permutational multivariate analysis of variance revealed differences in bacterial community composition and structure between non-inoculated and inoculated rhizospheres. Moreover, an influence of the plant species was observed. Different bacterial groups were found to be indicator taxonomic groups of non-inoculated and inoculated rhizospheres, Gemmatimonadetes and Anaerolineaceae, respectively, being the most notable indicators. As shown by distance based redundancy analysis, the shifts in bacterial community composition and structure mediated by the inoculation with the AM fungus were mainly related to changes in plant nutrients and growth parameters, such as the shoot phosphorus content. Our findings suggest that the AM fungal inoculum was able to modify the rhizosphere bacterial community assemblage while improving the host plant performance.
authors.affiliation: CSIC-Centro de Edafología y Biología Aplicada del Segura, Department of Soil and Water Conservation, P.O. Box 164, Campus de Espinardo, 30100 Murcia, Spain. Electronic address: grcaballero@cebas.csic.es.
authors.forename: G
authors.initials: G
authors.lastname: Rodríguez-Caballero
authors.affiliation: CSIC-Centro de Edafología y Biología Aplicada del Segura, Department of Soil and Water Conservation, P.O. Box 164, Campus de Espinardo, 30100 Murcia, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Caravaca
authors.affiliation: CSIC - Estación Experimental del Zaidín, Soil Microbiology and Symbiotic Systems Department, Profesor Albareda, 1, 18008 Granada, Spain.
authors.forename: A J
authors.initials: AJ
authors.lastname: Fernández-González
authors.affiliation: CSIC-Centro de Edafología y Biología Aplicada del Segura, Department of Soil and Water Conservation, P.O. Box 164, Campus de Espinardo, 30100 Murcia, Spain.
authors.forename: M M
authors.initials: MM
authors.lastname: Alguacil
authors.affiliation: CSIC - Estación Experimental del Zaidín, Soil Microbiology and Symbiotic Systems Department, Profesor Albareda, 1, 18008 Granada, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Fernández-López
authors.affiliation: CSIC-Centro de Edafología y Biología Aplicada del Segura, Department of Soil and Water Conservation, P.O. Box 164, Campus de Espinardo, 30100 Murcia, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Roldán
mesh_terms: D059827:Agricultural Inoculants; D019659:Asteraceae; D001419:Bacteria / Q000145:classification*; D017753:Ecosystem; D027523:Lavandula; D038821:Mycorrhizae / Q000502:physiology*; D018517:Plant Roots / Q000382:microbiology; D058441:Rhizosphere; D027544:Salvia; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D013030:Spain; D046930:Thymus Plant
chemical_list: D012987:Soil
keywords: Autochthonous shrub species; Microbial activity; Pyrosequencing; Rhizosphere bacterial community; Semiarid environment