title: MiR-125b inhibits anaplastic thyroid cancer cell migration and invasion by targeting PIK3CD.
abstract: Emerging evidences indicate that dysregulated microRNAs are implicated in the process of tumorigenesis and progression. The miRNA-125b (miR-125b) is downregulated and identified as tumor supressor in various cancers including thyroid cancer. However, the role and mechanism of miR-125b in anaplastic thyroid cancer (ATC) migration and invasion remain unknown. In the present study, the expression levels of miR-125b were downregulated and the expression levels of phosphoinositide 3-kinase catalytic subunit delta (PIK3CD) were upregulated in ATC tissues and cell lines. Moreover, miR-125b expression was negatively related to PIK3CD expression in ATC tissues. A computational search and luciferase assay identified PIK3CD as a direct target of miR-125b in ATC and PIK3CD expression was downregulated by miR-125b in ATC cells. In terms of function, miR-125b repressed migration and invasion of ATC cells, whereas PIK3CD overexpression reversed this effect. Furthermore, we showed that exogenous miR-125b decreased the PI3K, phospho-Akt, and phospho-mTOR expression in ATC cells. In conclusion, these results indicated that miR-125b suppressed ATC cell migration and invasion by targeting PIK3CD expression, and suggested novel potential therapeutic targets for the treatment of ATC.
authors.affiliation: Shengli Oilfield Central Hospital, Jinan Road, Dongying, Shandong Province No. 31, 257034, China. Electronic address: qgbuzxc@163.com.
authors.forename: Qingao
authors.initials: Q
authors.lastname: Bu
authors.affiliation: Shengli Oilfield Central Hospital, Jinan Road, Dongying, Shandong Province No. 31, 257034, China. Electronic address: fpyouzxc@163.com.
authors.forename: Faping
authors.initials: F
authors.lastname: You
authors.affiliation: Shengli Oilfield Central Hospital, Jinan Road, Dongying, Shandong Province No. 31, 257034, China. Electronic address: gzpanabc@163.com.
authors.forename: Guozheng
authors.initials: G
authors.lastname: Pan
authors.affiliation: Shengli Oilfield Central Hospital, Jinan Road, Dongying, Shandong Province No. 31, 257034, China. Electronic address: qzyuanzxc@163.com.
authors.forename: Qingzhong
authors.initials: Q
authors.lastname: Yuan
authors.affiliation: Shengli Oilfield Central Hospital, Jinan Road, Dongying, Shandong Province No. 31, 257034, China. Electronic address: tcuizxc@163.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Cui
authors.affiliation: Shengli Oilfield Central Hospital, Jinan Road, Dongying, Shandong Province No. 31, 257034, China. Electronic address: llhaozxc@163.com.
authors.forename: Long
authors.initials: L
authors.lastname: Hao
authors.affiliation: Shengli Oilfield Central Hospital, Jinan Road, Dongying, Shandong Province No. 31, 257034, China. Electronic address: jzhangzxc@163.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D058534:Class I Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D015398:Signal Transduction / Q000235:genetics; D065646:Thyroid Carcinoma, Anaplastic / Q000235:genetics* / Q000473:pathology*; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology*
chemical_list: C531888:MIRN125 microRNA, human; D035683:MicroRNAs; D058534:Class I Phosphatidylinositol 3-Kinases; C486018:PIK3CD protein, human
keywords: ATC; Invasion; Migration; PIK3CD; miR-125b