title: Development of a Novel Quantum Dots and Graphene Oxide Based FRET Assay for Rapid Detection ofinvAGene ofSalmonella.
abstract: A novel, rapid and simple fluorescence resonance energy transfer (FRET) basedSalmonellaspecific gene,invA, detection system was developed, in which quantum dots (QDs) and graphene oxide (GO) worked as fluorescent donor and quencher, respectively. By measuring the fluorescence intensity signal, theSalmonellaspecificinvAgene could be sensitively and specifically detected with a limit of detection (LOD) of ∼4 nM of theinvAgene in 20 min. The developed system has the potential to be used forSalmonelladetection in food and environmental samples and further developed into a platform for detection of other bacterial pathogens.
authors.affiliation: Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural UniversityGuangzhou, China; Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research InstituteShenzhen, China.
authors.forename: Jiubiao
authors.initials: J
authors.lastname: Guo
authors.affiliation: Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research InstituteShenzhen, China; State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic UniversityHung Hom, Hong Kong.
authors.forename: Edward W C
authors.initials: EW
authors.lastname: Chan
authors.affiliation: Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research InstituteShenzhen, China; State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic UniversityHung Hom, Hong Kong.
authors.forename: Sheng
authors.initials: S
authors.lastname: Chen
authors.affiliation: Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University Guangzhou, China.
authors.forename: Zhenling
authors.initials: Z
authors.lastname: Zeng
keywords: FRET assay; Salmonella; graphene oxide; invA; quantum dots; rapid detection