title: Identification of RAN1 orthologue associated with sex determination through whole genome sequencing analysis in fig (Ficus carica L.).
abstract: With the aim of identifying sex determinants of fig, we generated the first draft genome sequence of fig and conducted the subsequent analyses. Linkage analysis with a high-density genetic map established by a restriction-site associated sequencing technique, and genome-wide association study followed by whole-genome resequencing analysis identified two missense mutations in RESPONSIVE-TO-ANTAGONIST1 (RAN1) orthologue encoding copper-transporting ATPase completely associated with sex phenotypes of investigated figs. This result suggests that RAN1 is a possible sex determinant candidate in the fig genome. The genomic resources and genetic findings obtained in this study can contribute to general understanding of Ficus species and provide an insight into fig's and plant's sex determination system.
authors.affiliation: Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Mori
authors.affiliation: Kazusa DNA Research Institute, Kisarazu, Chiba, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Shirasawa
authors.affiliation: Fukuoka Agriculture and Forestry Research Center Buzen Branch, Yukuhashi, Fukuoka, Japan.|Fukuoka Keichiku Agricultural Extension Center, Fukuoka, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nogata
authors.affiliation: Fukuoka Agriculture and Forestry Research Center, Fukuoka, Japan.
authors.forename: Chiharu
authors.initials: C
authors.lastname: Hirata
authors.affiliation: Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Tashiro
authors.affiliation: Graduate School of Agriculture, Ehime University, Ehime, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Habu
authors.affiliation: Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
authors.forename: Sangwan
authors.initials: S
authors.lastname: Kim
authors.affiliation: Fukuoka Agriculture and Forestry Research Center Buzen Branch, Yukuhashi, Fukuoka, Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Himeno
authors.affiliation: Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Kuhara
authors.affiliation: Fukuoka Agriculture and Forestry Research Center Buzen Branch, Yukuhashi, Fukuoka, Japan. ikegami@farc.pref.fukuoka.jp.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Ikegami
mesh_terms: D027682:Cation Transport Proteins / Q000235:genetics*; D030681:Ficus / Q000235:genetics*; D055106:Genome-Wide Association Study; D050505:Mutant Proteins / Q000235:genetics*; D020125:Mutation, Missense; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics*; D000073336:Whole Genome Sequencing
chemical_list: D027682:Cation Transport Proteins; D050505:Mutant Proteins; D010940:Plant Proteins