title: Determining the Enzymatic Activity of Angiotensin-Converting Enzyme 2 (ACE2) in Brain Tissue and Cerebrospinal Fluid Using a Quenched Fluorescent Substrate.
abstract: Angiotensin-converting enzyme 2 (ACE2) is a component of the renin-angiotensin system (RAS) which plays an important role in the regulation of blood pressure and volume homeostasis. Accumulating evidence shows alterations in ACE2 expression and activity in several hypertensive animal models, as well as in patients with hypertension. In order to assess the role of brain ACE2 in hypertension, a specific ACE2 assay is required. Based on a quenched fluorescent substrate, we describe an easy-to-use method for determining ACE2 activity in brain tissue and cerebrospinal fluid. The method can further be adapted for other tissues, plasma, cell extracts, and cell culture supernatants.
authors.affiliation: Department of Pharmacology and Experimental Therapeutics, Cardiovascular Center of Excellence, Louisiana State University Health Sciences Center, 1901 Perdido Street, New Orleans, LA, 70112, USA.
authors.forename: Srinivas
authors.initials: S
authors.lastname: Sriramula
authors.affiliation: Department of Pharmacology and Experimental Therapeutics, Cardiovascular Center of Excellence, Louisiana State University Health Sciences Center, 1901 Perdido Street, New Orleans, LA, 70112, USA.
authors.forename: Kim Brint
authors.initials: KB
authors.lastname: Pedersen
authors.affiliation: Department of Pharmacology and Experimental Therapeutics, Cardiovascular Center of Excellence, Louisiana State University Health Sciences Center, 1901 Perdido Street, New Orleans, LA, 70112, USA.
authors.forename: Huijing
authors.initials: H
authors.lastname: Xia
authors.affiliation: Department of Pharmacology and Experimental Therapeutics, Cardiovascular Center of Excellence, Louisiana State University Health Sciences Center, 1901 Perdido Street, New Orleans, LA, 70112, USA. elazar@lsuhsc.edu.
authors.forename: Eric
authors.initials: E
authors.lastname: Lazartigues
mesh_terms: D000085962:Angiotensin-Converting Enzyme 2; D000818:Animals; D001794:Blood Pressure / Q000235:genetics / Q000502:physiology; D001921:Brain / Q000378:metabolism*; D057075:Enzyme Assays / Q000379:methods*; D006801:Humans; D006973:Hypertension / Q000134:cerebrospinal fluid / Q000378:metabolism; D010455:Peptides; D007703:Peptidyl-Dipeptidase A / Q000134:cerebrospinal fluid / Q000378:metabolism*; D012084:Renin-Angiotensin System / Q000502:physiology
chemical_list: C556151:DX600 peptide; D010455:Peptides; D007703:Peptidyl-Dipeptidase A; C000705307:ACE2 protein, human; D000085962:Angiotensin-Converting Enzyme 2
keywords: ACE2; Brain ACE2; CSF; DX600; Hypertension; Mca-APK(Dnp); Quenched fluorescent substrate