title: Wisteria floribunda Agglutinin and Its Reactive-Glycan-Carrying Prostate-Specific Antigen as a Novel Diagnostic and Prognostic Marker of Prostate Cancer.
abstract: Wisteria floribundaagglutinin (WFA) preferably binds to LacdiNAc glycans, and its reactivity is associated with tumor progression. The aim of this study to examine whether the serum LacdiNAc carrying prostate-specific antigen-glycosylation isomer (PSA-Gi) and WFA-reactivity of tumor tissue can be applied as a diagnostic and prognostic marker of prostate cancer (PCa). Between 2007 and 2016, serum PSA-Gi levels before prostate biopsy (Pbx) were measured in 184 biopsy-proven benign prostatic hyperplasia patients and 244 PCa patients using an automated lectin-antibody immunoassay. WFA-reactivity on tumor was analyzed in 260 radical prostatectomy (RP) patients. Diagnostic and prognostic performance of serum PSA-Gi was evaluated using area under the receiver-operator characteristic curve (AUC). Prognostic performance of WFA-reactivity on tumor was evaluated via Cox proportional hazards regression analysis and nomogram. The AUC of serum PSA-Gi detecting PCa and predicting Pbx Grade Group (GG) 3 and GG ≥ 3 after RP was much higher than those of conventional PSA. Multivariate analysis showed that WFA-reactivity on prostate tumor was an independent risk factor of PSA recurrence. The nomogram was a strong model for predicting PSA-free survival provability with ac-index ≥0.7. Serum PSA-Gi levels and WFA-reactivity on prostate tumor may be a novel diagnostic and pre- and post-operative prognostic biomarkers of PCa, respectively.
authors.affiliation: Department of Urology, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan. hagiwara.kazuhisa@gmail.com.
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Hagiwara
authors.affiliation: Department of Urology, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan. tobisawa@hirosaki-u.ac.jp.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Tobisawa
authors.affiliation: Corporate R&D Headquarters, Konica Minolta, Inc., Hino-shi, Tokyo 191-8511, Japan. takatoshi.kaya@konicaminolta.com.
authors.forename: Takatoshi
authors.initials: T
authors.lastname: Kaya
authors.affiliation: Corporate R&D Headquarters, Konica Minolta, Inc., Hino-shi, Tokyo 191-8511, Japan. tomonori.kaneko1@konicaminolta.com.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Kaneko
authors.affiliation: Department of Urology, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan. shingoh@hirosaki-u.ac.jp.
authors.forename: Shingo
authors.initials: S
authors.lastname: Hatakeyama
authors.affiliation: Department of Urology, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan. moribio@hirosaki-u.ac.jp.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Mori
authors.affiliation: Department of Advanced Transplant and Regenerative Medicine, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan. bikkuri@opal.plala.or.jp.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Hashimoto
authors.affiliation: Department of Urology, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan. goodwin@hirosaki-u.ac.jp.
authors.forename: Takuya
authors.initials: T
authors.lastname: Koie
authors.affiliation: Corporate R&D Headquarters, Konica Minolta, Inc., Hino-shi, Tokyo 191-8511, Japan. yoshihiko.suda@konicaminolta.com.
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Suda
authors.affiliation: Department of Urology, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan. coyama@hirosaki-u.ac.jp.|Department of Advanced Transplant and Regenerative Medicine, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan. coyama@hirosaki-u.ac.jp.
authors.forename: Chikara
authors.initials: C
authors.lastname: Ohyama
authors.affiliation: Department of Urology, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan. tohruyon@hirosaki-u.ac.jp.|Department of Advanced Transplant and Regenerative Medicine, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan. tohruyon@hirosaki-u.ac.jp.
authors.forename: Tohru
authors.initials: T
authors.lastname: Yoneyama
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood*; D001706:Biopsy; D006031:Glycosylation; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D060787:Neoplasm Grading; D009364:Neoplasm Recurrence, Local / Q000473:pathology; D037121:Plant Lectins / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D011379:Prognosis; D011467:Prostate / Q000473:pathology / Q000601:surgery; D017430:Prostate-Specific Antigen / Q000097:blood / Q000378:metabolism*; D011468:Prostatectomy; D011470:Prostatic Hyperplasia / Q000097:blood / Q000473:pathology; D011471:Prostatic Neoplasms / Q000097:blood* / Q000175:diagnosis* / Q000473:pathology / Q000601:surgery; D012372:ROC Curve; D034781:Receptors, N-Acetylglucosamine / Q000378:metabolism*; D012307:Risk Factors
chemical_list: D014408:Biomarkers, Tumor; D037121:Plant Lectins; D011134:Polysaccharides; D034781:Receptors, N-Acetylglucosamine; C040129:wisteria lectin; D017430:Prostate-Specific Antigen
keywords: LacdiNAc; N-glycan; Wisteria floribunda agglutinin (WFA) lectin; biomarker; prostate-specific antigen