title: [Expression and localization of FGFR family in squamous cell carcinoma of head and neck].
abstract: OBJECTIVE
  To investigate the expression and localization of FGFR family in squamous cell carcinoma of head and neck (SCCHN) cell lines.
  
  
  METHODS
  Total protein was extracted from 10 SCCHN cell lines and the expression of FGFR was detected by Western blot. The localization of FGFR was further demonstrated by immunofluorescence staining in SCC25 and HN4 cell lines. Gray value was measured by Image J. GraphPad Prism 5.01 software package was used for data processing and analysis.
  
  
  RESULTS
  FGFR1 expression was detected in 6/10 cell lines and FGFR2, 3, 4 was detectable in all cell lines. The expression of FGFR1, 2, 4 was mainly in the nucleus and cytoplasm while FGFR3 was predominantly localized in cytoplasm.
  
  
  CONCLUSIONS
  FGFR shows co-expression in SCCHN cell lines, which may be associated with the tumorigenesis and development of SCCHN.
authors.affiliation: Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology. Shanghai 200011, China. E-mail:scjtdcq@126.com.
authors.forename: Chao-Ji
authors.initials: CJ
authors.lastname: Shi
authors.forename: Rong
authors.initials: R
authors.lastname: Zhou
authors.forename: Yong
authors.initials: Y
authors.lastname: Han
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Tao
authors.forename: Shu-Yang
authors.initials: SY
authors.lastname: Sun
authors.forename: Zhi-Yuan
authors.initials: ZY
authors.lastname: Zhang
mesh_terms: D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D006258:Head and Neck Neoplasms / Q000378:metabolism*; D006801:Humans; D012333:RNA, Messenger; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000378:metabolism; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000378:metabolism; D051498:Receptor, Fibroblast Growth Factor, Type 3; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: D012333:RNA, Messenger; C496348:FGFR1 protein, human; C496353:FGFR2 protein, human; C496365:FGFR3 protein, human; D051496:Receptor, Fibroblast Growth Factor, Type 1; D051497:Receptor, Fibroblast Growth Factor, Type 2; D051498:Receptor, Fibroblast Growth Factor, Type 3