title: Evaluation of a novel ELISA for the tumor-associated antigen CA 72-4 in patients with ovarian cancer.
abstract: OBJECTIVE
  Cancer antigen 72-4 (CA 72-4) is an established tumor marker in ovarian cancer. We evaluated a new solid-phase ELISA (DRG TM-CA 72-4 ELISA).
  
  
  METHODS
  Repeated measures of test samples and controls were performed to evaluate reliability and reproducibility. Afterward, we performed analyses on the sera of 150 patients with primarily diagnosed ovarian cancer. Results were compared with those of the Cobas CA 72-4 kit. Results were correlated with clinical patient data.
  
  
  RESULTS
  Results of the DRG TM-CA 72-4 ELISA were reproducible with acceptable deviations within measures, and the measured CA 72-4 serum concentrations were well in accordance with the references. High concentrations were significantly associated with grading, tumor stage and tumor residuals after surgery.
authors.affiliation: Department of Obstetrics & Gynecology, University Hospital Essen, Hufelandstr. 55, 45147 Essen, Germany; Department of Obstetrics & Gynecology, University Hospital Essen, Hufelandstr. 55, 45147 Essen, Germany.
authors.forename: Paul
authors.initials: P
authors.lastname: Buderath
authors.affiliation: Department of Obstetrics & Gynecology, University Hospital Essen, Hufelandstr. 55, 45147 Essen, Germany; Department of Obstetrics & Gynecology, University Hospital Essen, Hufelandstr. 55, 45147 Essen, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Kasimir-Bauer
authors.affiliation: Department of Obstetrics & Gynecology, University Hospital Essen, Hufelandstr. 55, 45147 Essen, Germany; Department of Obstetrics & Gynecology, University Hospital Essen, Hufelandstr. 55, 45147 Essen, Germany.
authors.forename: Bahriye
authors.initials: B
authors.lastname: Aktas
authors.affiliation: Department of Obstetrics & Gynecology, University Hospital Essen, Hufelandstr. 55, 45147 Essen, Germany; Department of Obstetrics & Gynecology, University Hospital Essen, Hufelandstr. 55, 45147 Essen, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Rasch
authors.affiliation: Department of Obstetrics & Gynecology, University Hospital Essen, Hufelandstr. 55, 45147 Essen, Germany; Department of Obstetrics & Gynecology, University Hospital Essen, Hufelandstr. 55, 45147 Essen, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Kimmig
authors.affiliation: Department of Obstetrics & Gynecology, University Hospital Essen, Hufelandstr. 55, 45147 Essen, Germany; DRG Instruments GmbH, Frauenbergstraße 18, 35039 Marburg, Germany; Department of Obstetrics & Gynecology, University Hospital Essen, Hufelandstr. 55, 45147 Essen, Germany; DRG Instruments GmbH, Frauenbergstraße 18, 35039 Marburg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Zeller
authors.affiliation: Department of Obstetrics & Gynecology, University Hospital Essen, Hufelandstr. 55, 45147 Essen, Germany; Department of Obstetrics & Gynecology, University Hospital Essen, Hufelandstr. 55, 45147 Essen, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Heubner
keywords: CA 72–4; ELISA; diagnostics; ovarian cancer; tumor markers