title: Dynamic changes in biomarkers in acute human immunodeficiency virus infections: a case report.
abstract: BACKGROUND
  The highest incidence of human immunodeficiency virus infection in China is among men who have sex with men. This case report aims to describe the dynamic changes in biomarkers in an acute human immunodeficiency virus infection of a Han Chinese man who has sex with men, and to illustrate the possibility of using these biomarkers for the early detection of human immunodeficiency virus infection in Chinese hospital settings.
  
  
  METHODS
  The 25-year-old Han Chinese male patient presented himself with an 8-day history of symptoms and signs of upper respiratory viral infections to a sexually transmitted infection clinic of a hospital setting in Shanghai. The viral load of human immunodeficiency virus, p24 antigen-antibody complex, and lymphocyte subsets of blood samples were repeatedly measured over the next 39 days. The human immunodeficiency virus from serum was genotyped. This patient was diagnosed as a human immunodeficiency virus infection, and the viral genotype was CRF 01_AE. The onset of the symptoms and signs was 12 days after his last reported unprotected intercourse with a human immunodeficiency virus -infected man. The patient had detectable levels of p24 antigen at his first visit, 20 days after infection, and the HIV viral load was at the highest point (8 × 106copies/ml). A low concentration of antibody to HIV was observed in the patient's serum 10 days after his 1st visit (30 days after infection). The confirmation of human immunodeficiency virus infection by Western blot assays was made at day 20 after his 1st visit (40 days after infection).
  
  
  CONCLUSIONS
  Symptoms of acute human immunodeficiency virus infection are non-specific. Specific laboratory markers appear shortly after HIV infections. The first biomarker detected from serum is the viral RNA and p24 antigen, followed by HIV-specific antibody. The results suggest that there are urgent needs for both human immunodeficiency virus antigen and antibody testing in routine medical practice, and that human immunodeficiency virus RNA testing should be recommended to detect early infection. Ethics approval was obtained from the Ethics Board of the Shanghai Dermatology Hospital.
authors.affiliation: Shanghai Dermatology Hospital, Shanghai, 200050, China. weiming_gu2003@aliyun.com.
authors.forename: Wei-Ming
authors.initials: WM
authors.lastname: Gu
authors.affiliation: School of Public Health, Fudan University, Shanghai, 200032, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Shanghai Dermatology Hospital, Shanghai, 200050, China.
authors.forename: Wei-Zhong
authors.initials: WZ
authors.lastname: Hu
authors.affiliation: School of Public Health, Fudan University, Shanghai, 200032, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Xu
mesh_terms: D000208:Acute Disease; D000328:Adult; D015415:Biomarkers / Q000097:blood*; D015658:HIV Infections / Q000097:blood*; D006801:Humans; D008297:Male
chemical_list: D015415:Biomarkers
keywords: Acute infection; Antibody testing; Biomarkers; China; HIV case report; Men who have sex with men; p24 antigen