title: Dietary Tryptophan Enhanced the Expression of Tight Junction Protein ZO-1 in Intestine.
abstract: Dietary amino acids provide various beneficial effects for our health. The aim of the present study was to assess the effects of tryptophan (Trp) supplementation on barrier function. Ninety-six healthy finishing pigs (initial body weight 51.49 ± 1.12 kg) were randomly allocated into 2 treatment groups, control group, and 0.2% Trp group. The control group was fed the basal diet, and 0.2% Trp group was fed basal diet plus 0.2% Trp. The trial period is 60 d. Compared with control group, the mRNA abundance of claudin-3 and zonula occluden-1 (ZO-1) in the jejunum in 0.2% Trp group (P < 0.05) was increased. According to immunohistochemistry and immunoblotting test, the expression of ZO-1 in jejunum in 0.2% Trp group was also significantly increased compared with the control group (P < 0.05). These results revealed that Trp enhanced the expression of tight junction protein ZO-1 in the intestine of pig model. Trp may be potential and beneficial dietary functional factor for regulating the intestinal development and inhibiting intestinal aging.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang Univ., Nanchang, 330047, China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang Univ., Nanchang, 330047, China.
authors.forename: Shumei
authors.initials: S
authors.lastname: Mi
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang Univ., Nanchang, 330047, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Ruan
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang Univ., Nanchang, 330047, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang Univ., Nanchang, 330047, China.
authors.forename: Xugang
authors.initials: X
authors.lastname: Shu
authors.affiliation: Inst. of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang Univ., Nanchang, 330047, China.
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang Univ., Nanchang, 330047, China.
authors.forename: Zeyuan
authors.initials: Z
authors.lastname: Deng
mesh_terms: D000821:Animal Feed; D000818:Animals; D001835:Body Weight; D062465:Claudin-3 / Q000378:metabolism; D004032:Diet; D019587:Dietary Supplements; D004731:Endotoxins / Q000737:chemistry; D007413:Intestinal Mucosa / Q000378:metabolism*; D010539:Permeability; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D015398:Signal Transduction / Q000187:drug effects; D013552:Swine; D019108:Tight Junctions / Q000378:metabolism; D014364:Tryptophan / Q000737:chemistry*; D062826:Zonula Occludens-1 Protein / Q000378:metabolism*
chemical_list: D062465:Claudin-3; D004731:Endotoxins; D012333:RNA, Messenger; D062826:Zonula Occludens-1 Protein; D014364:Tryptophan
keywords: amino acids; intestinal permeability; tight junction; tryptophan; zonula occluden-1