title: Allosteric regulation of helicase core activities of the DEAD-box helicase YxiN by RNA binding to its RNA recognition motif.
abstract: DEAD-box proteins share a structurally similar core of two RecA-like domains (RecA_N and RecA_C) that contain the conserved motifs for ATP-dependent RNA unwinding. In many DEAD-box proteins the helicase core is flanked by ancillary domains. To understand the regulation of the DEAD-box helicase YxiN by its C-terminal RNA recognition motif (RRM), we investigated the effect of RNA binding to the RRM on its position relative to the core, and on core activities. RRM/RNA complex formation substantially shifts the RRM from a position close to the RecA_C to the proximity of RecA_N, independent of RNA contacts with the core. RNA binding to the RRM is communicated to the core, and stimulates ATP hydrolysis and RNA unwinding. The conformational space of the core depends on the identity of the RRM-bound RNA. Allosteric regulation of core activities by RNA-induced movement of ancillary domains may constitute a general regulatory mechanism of DEAD-box protein activity.
authors.affiliation: Institute for Physical Chemistry, University of Muenster, Correnstrasse 30, 48149 Muenster, Germany.
authors.forename: Brighton
authors.initials: B
authors.lastname: Samatanga
authors.affiliation: Institute for Physical Chemistry, University of Muenster, Correnstrasse 30, 48149 Muenster, Germany.
authors.forename: Alexandra Z
authors.initials: AZ
authors.lastname: Andreou
authors.affiliation: Institute for Physical Chemistry, University of Muenster, Correnstrasse 30, 48149 Muenster, Germany.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Klostermeier
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000494:Allosteric Regulation; D000595:Amino Acid Sequence; D001665:Binding Sites; D053487:DEAD-box RNA Helicases / Q000737:chemistry* / Q000378:metabolism*; D006868:Hydrolysis; D008390:Markov Chains; D008958:Models, Molecular; D008968:Molecular Conformation; D009690:Nucleic Acid Conformation; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D012313:RNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000071377:RNA Recognition Motif
chemical_list: D010455:Peptides; D012313:RNA; D000255:Adenosine Triphosphate; D053487:DEAD-box RNA Helicases