title: Protocol for Producing Synthetic Polyploid Oats.
abstract: A protocol to produce synthetic oats by introducing alien genetic variations into cultivated oats is proposed based on suitable genetic relationships between Avena species. Furthermore, artificial hybridization procedures between different ploidy species are explained. Amphiploids can be produced by rescuing aborted embryos and treating the F1hybrids with colchicine to overcome sterility between interspecific plants. Furthermore, I present the cytological methods for observing somatic and meiotic chromosomes in the treated hybrids.
authors.affiliation: Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai city, Osaka, 599-8531, Japan. morikawa@plant.osakafu-u.ac.jp.
authors.forename: Toshinobu
authors.initials: T
authors.lastname: Morikawa
mesh_terms: D018554:Avena / Q000235:genetics* / Q000378:metabolism; D001947:Breeding; D018014:Gene Transfer Techniques; D006824:Hybridization, Genetic; D054817:Pollination; D011123:Polyploidy
keywords: Amphiploid; Avena sativa; Chromosome doubling; Colchicine; Emasculation; Embryo rescue; Hybridization; Pollination; Polyploid; Synthetic oats