title: The expression of VEGF, myoglobin and CRP2 proteins regulating endometrial remodeling in the porcine endometrial tissues during follicular and luteal phase.
abstract: Endometrial remodeling is important for successful embryo development and implantation in pigs. Therefore, this study investigated change of proteins regulating endometrial remodeling on follicular and luteal phase in porcine endometrial tissues. The endometrial tissue samples were collected from porcine uterus during follicular and luteal phase, vascular endothelial growth factor (VEGF), myoglobin and cysteine-rich protein 2 (CRP2) proteins were expressed by immnofluorescence, immunoblotting, and determined by 2-DE and MALDI-TOF/MS. We found that VEGF, myoglobin and CRP2 were strongly localized in endometrial tissues during luteal phase, but not follicular phase. The protein levels of VEGF, myoglobin and CRP2 in endometrial tissues were higher than luteal phase (P < 0.05). These results may provide understanding of intrauterine environment during estrous cycle in pigs, and will be used in animal reproduction for developing specific biomarkers in the future.
authors.affiliation: College of Animal Life Sciences, Kangwon National University, Chuncheon, Republic of Korea.
authors.forename: Seunghyung
authors.initials: S
authors.lastname: Lee
authors.affiliation: College of Animal Life Sciences, Kangwon National University, Chuncheon, Republic of Korea.
authors.forename: Sang-Hee
authors.initials: SH
authors.lastname: Lee
authors.affiliation: College of Animal Life Sciences, Kangwon National University, Chuncheon, Republic of Korea.
authors.forename: Boo-Keun
authors.initials: BK
authors.lastname: Yang
authors.affiliation: College of Animal Life Sciences, Kangwon National University, Chuncheon, Republic of Korea.
authors.forename: Choon-Keun
authors.initials: CK
authors.lastname: Park
mesh_terms: D000818:Animals; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000378:metabolism* / Q000502:physiology; D010064:Embryo Implantation / Q000235:genetics / Q000502:physiology; D047108:Embryonic Development / Q000235:genetics / Q000502:physiology; D004717:Endometrium / Q000378:metabolism*; D030762:Estrous Cycle / Q000235:genetics / Q000502:physiology; D005260:Female; D005498:Follicular Phase / Q000235:genetics* / Q000378:metabolism*; D008183:Luteal Phase / Q000235:genetics* / Q000378:metabolism*; D009211:Myoglobin / Q000378:metabolism* / Q000502:physiology; D013552:Swine / Q000378:metabolism* / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism* / Q000502:physiology
chemical_list: D022782:CCAAT-Enhancer-Binding Protein-beta; D009211:Myoglobin; D042461:Vascular Endothelial Growth Factor A
keywords: endometrial gland; endometrial tissue; extracellular matrix; follicular phase; luteal phase