title: Comparative Proteomic and Nutritional Composition Analysis of Independent Transgenic Pigeon Pea Seeds Harboring cry1AcF and cry2Aa Genes and Their Nontransgenic Counterparts.
abstract: Safety assessment of genetically modified plants is an important aspect prior to deregulation. Demonstration of substantial equivalence of the transgenics compared to their nontransgenic counterparts can be performed using different techniques at various molecular levels. The present study is a first-ever comprehensive evaluation of pigeon pea transgenics harboring two independent cry genes, cry2Aa and cry1AcF. The absence of unintended effects in the transgenic seed components was demonstrated by proteome and nutritional composition profiling. Analysis revealed that no significant differences were found in the various nutritional compositional analyses performed. Additionally, 2-DGE-based proteome analysis of the transgenic and nontransgenic seed protein revealed that there were no major changes in the protein profile, although a minor fold change in the expression of a few proteins was observed. Furthermore, the study also demonstrated that neither the integration of T-DNA nor the expression of the cry genes resulted in the production of unintended effects in the form of new toxins or allergens.
authors.affiliation: ICAR-National Research Centre on Plant Biotechnology , New Delhi 110012, India.
authors.forename: Pragya
authors.initials: P
authors.lastname: Mishra
authors.affiliation: ICAR-National Research Centre on Plant Biotechnology , New Delhi 110012, India.
authors.forename: Shweta
authors.initials: S
authors.lastname: Singh
authors.affiliation: ICAR-National Research Centre on Plant Biotechnology , New Delhi 110012, India.
authors.forename: Maniraj
authors.initials: M
authors.lastname: Rathinam
authors.affiliation: ICAR-National Research Centre on Plant Biotechnology , New Delhi 110012, India.
authors.forename: Muralimohan
authors.initials: M
authors.lastname: Nandiganti
authors.affiliation: ICAR-National Research Centre on Plant Biotechnology , New Delhi 110012, India.
authors.forename: Nikhil
authors.initials: N
authors.lastname: Ram Kumar
authors.affiliation: ICAR-National Research Centre on Plant Biotechnology , New Delhi 110012, India.
authors.forename: Arulprakash
authors.initials: A
authors.lastname: Thangaraj
authors.affiliation: Division of Biochemistry, ICAR-Indian Agricultural Research Institute , New Delhi 110012, India.
authors.forename: Vinutha
authors.initials: V
authors.lastname: Thimmegowda
authors.affiliation: Division of Biochemistry, ICAR-Indian Agricultural Research Institute , New Delhi 110012, India.
authors.forename: Veda
authors.initials: V
authors.lastname: Krishnan
authors.affiliation: ICAR-National Research Centre on Plant Biotechnology , New Delhi 110012, India.
authors.forename: Vagish
authors.initials: V
authors.lastname: Mishra
authors.affiliation: ICAR-National Research Centre on Plant Biotechnology , New Delhi 110012, India.
authors.forename: Neha
authors.initials: N
authors.lastname: Jain
authors.affiliation: ICAR-National Research Centre on Plant Biotechnology , New Delhi 110012, India.
authors.forename: Vandna
authors.initials: V
authors.lastname: Rai
authors.affiliation: ICAR-National Research Centre on Plant Biotechnology , New Delhi 110012, India.
authors.forename: Debasis
authors.initials: D
authors.lastname: Pattanayak
authors.affiliation: ICAR-National Research Centre on Plant Biotechnology , New Delhi 110012, India.
authors.forename: Rohini
authors.identifier: http://orcid.org/0000-0003-4171-6956
authors.initials: R
authors.lastname: Sreevathsa
mesh_terms: D000596:Amino Acids / Q000032:analysis / Q000378:metabolism; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D036561:Cajanus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004731:Endotoxins / Q000235:genetics* / Q000378:metabolism; D006460:Hemolysin Proteins / Q000235:genetics* / Q000378:metabolism; D008903:Minerals / Q000032:analysis / Q000378:metabolism; D009753:Nutritive Value; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000235:genetics; D040901:Proteomics; D012639:Seeds / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D000596:Amino Acids; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D008903:Minerals; D010940:Plant Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis
keywords: nutritional analysis; pigeon pea; proteome; transgenics