title: Response of genes involved in lipid metabolism in rat epididymal white adipose tissue to different fasting conditions after long-term fructose consumption.
abstract: There has been much concern regarding the dietary fructose contributes to the development of metabolic syndrome. High-fructose diet changes the expression of genes involved in lipid metabolism. Levels of a number of hepatic lipogenic enzymes are increased by a high-carbohydrate diet in fasted-refed model rats/mice. Both the white adipose tissue (WAT) and the liver play a key role in the maintenance of nutrient homeostasis. Here, the aim of this study was to analyze the expression of key genes related to lipid metabolism in epididymal WAT (eWAT) in response to different fasting condition after long-term chronic fructose consumption. Rats were fed standard chow supplemented with 10% w/v fructose solution for 5 weeks, and killed after chow-fasting and fructose withdrawal (fasting) or chow-fasting and continued fructose (fructose alone) for 14 h. Blood parameters and the expression of genes involved in fatty acid synthesis (ChREBP, SREBP-1c, FAS, SCD1), triglyceride biosynthesis (DGAT-1, DGAT-2) and lipid mobilization (ATGL, HSL) in eWAT were analyzed. In addition, mRNA levels of PPAR-γ, CD36 and LPL were also detected. As expected, fructose alone increased the mRNA expression of FAS, SCD1, and correspondingly decreased ATGL and HSL mRNA levels. However, ChREBP, DGAT-2, ATGL and HSL mRNA levels restored near to normal while FAS and SCD1 tend to basic level under fasting condition. The mRNA expression of SREBP-1c, PPAR-γ and LPL did not changed at any situations but CD36 mRNA decreased remarkably in fructose alone group. In conclusion, these findings demonstrate that genes involved in lipid metabolism in rat eWAT are varied in response to different fasting conditions after long-term fructose consumption.
authors.affiliation: Chongqing Key Laboratory of Traditional Chinese Medicine for Prevention and Cure of Metabolic Diseases, Chongqing Medical University, 400016 China.
authors.forename: Jin-Xiu
authors.initials: JX
authors.lastname: Li
authors.affiliation: The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010 China.
authors.forename: Da-Zhi
authors.initials: DZ
authors.lastname: Ke
authors.affiliation: Chongqing Key Laboratory of Traditional Chinese Medicine for Prevention and Cure of Metabolic Diseases, Chongqing Medical University, 400016 China.
authors.forename: Ling
authors.initials: L
authors.lastname: Yao
authors.affiliation: Faculty of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016 China.
authors.forename: Shang
authors.initials: S
authors.lastname: Wang
authors.affiliation: Faculty of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016 China.
authors.forename: Peng
authors.initials: P
authors.lastname: Ma
authors.affiliation: Faculty of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016 China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016 China.
authors.forename: Guo-Wei
authors.initials: GW
authors.lastname: Zuo
authors.affiliation: Faculty of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016 China.
authors.forename: Li-Rong
authors.initials: LR
authors.lastname: Jiang
authors.affiliation: Chongqing Key Laboratory of Traditional Chinese Medicine for Prevention and Cure of Metabolic Diseases, Chongqing Medical University, 400016 China. Electronic address: wjwcq68@163.com.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Wang
mesh_terms: D052436:Adipose Tissue, White / Q000378:metabolism*; D000818:Animals; D001835:Body Weight; D004822:Epididymis / Q000378:metabolism*; D005215:Fasting; D005632:Fructose / Q000008:administration & dosage*; D015870:Gene Expression; D050356:Lipid Metabolism / Q000235:genetics*; D008297:Male; D051379:Mice; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005632:Fructose
keywords: Fasting; Fructose; Genes; Lipid metabolism; eWAT (epididymal white adipose tissue)