title: Role of Annexin A2 in the EGF-induced epithelial-mesenchymal transition in human CaSki cells.
abstract: The epidermal growth factor receptor (EGF-R) signaling pathway is thought to have an important role in the development and progression of several carcinomas, as it is associated with cell proliferation, differentiation and migration. Activation of EGF-R signaling regulates epithelial-mesenchymal transition (EMT)-associated invasion and migration in normal and malignant epithelial cells. However, the specific mechanisms have not yet been fully elucidated. The present study utilized wound healing assays, western blotting, flow cytometry and MTT assays to demonstrate that Annexin A2 (ANXA2) is a key regulatory factor in EGF-induced EMT in CaSki cervical cancer cells. Moreover, the increased expression levels of ANXA2 promoted cell viability and migration in human CaSki cells. It was also found that silencing ANXA2 partially reverses EGF-induced EMT and inhibits cell viability and migration in CaSki cells. These findings suggest that ANXA2 is a key regulator of EGF-induced EMT in CaSki cervical cancer cells.
authors.affiliation: Department of General Surgery, Yichang Central People's Hospital, The First College of Clinical Medical Science, Three Gorges University, Yichang, Hubei 443002, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Cui
authors.affiliation: Department of Biochemistry, School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei 430071, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Song
authors.affiliation: Institute of Molecular Biology of Three Gorges University, Yichang, Hubei 443002, P.R. China.
authors.forename: Liting
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of General Surgery, Yichang Central People's Hospital, The First College of Clinical Medical Science, Three Gorges University, Yichang, Hubei 443002, P.R. China.
authors.forename: Luhui
authors.initials: L
authors.lastname: Cheng
authors.affiliation: Department of General Surgery, Yichang Central People's Hospital, The First College of Clinical Medical Science, Three Gorges University, Yichang, Hubei 443002, P.R. China.
authors.forename: Aijun
authors.initials: A
authors.lastname: Chen
authors.affiliation: Institute of Molecular Biology of Three Gorges University, Yichang, Hubei 443002, P.R. China.
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Molecular Biology of Three Gorges University, Yichang, Hubei 443002, P.R. China.
authors.forename: Yingdi
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Institute of Molecular Biology of Three Gorges University, Yichang, Hubei 443002, P.R. China.
authors.forename: Liming
authors.initials: L
authors.lastname: Huang
keywords: Annexin A2; cell proliferation; epidermal growth factor receptor signaling; epithelial-mesenchymal transition