title: Aurora Kinase B Phosphorylates Histone H3.3 at Serine 31 during Mitosis in Mammalian Cells.
abstract: Serine 31 of histone H3.3 is phosphorylated during mitosis in mammalian cells and has been shown to influence chromosome segregation. We established a screening system to identify candidate kinase(s) phosphorylating H3.3S31 by using siRNA library targeting 720 human kinases, and we confirmed the results with an independent kinase inhibitor screen. Aurora kinase B was the best candidate hit in both screens. Moreover, the kinase activity of Aurora B on H3.3S31 was biochemically confirmed with nucleosomal substrates in vitro.
authors.affiliation: Peking University-Tsinghua University-National Institute of Biological Sciences Joint Graduate Program, School of Life Sciences, Peking University, Beijing, 100081, China; National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; National Institute of Biological Sciences, Beijing 102206, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Li
authors.affiliation: National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Dong
authors.affiliation: National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: zhubing@ibp.ac.cn.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhu
mesh_terms: D064107:Aurora Kinase B / Q000378:metabolism*; D005786:Gene Expression Regulation; D020868:Gene Silencing; D005820:Genetic Testing; D006657:Histones / Q000378:metabolism*; D006801:Humans; D008938:Mitosis; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D012694:Serine / Q000378:metabolism*
chemical_list: D006657:Histones; D012694:Serine; C576921:AURKB protein, human; D064107:Aurora Kinase B
keywords: Aurora kinase B; H3.3S31ph; histone phosphorylation; siRNA screen