title: Serum Metabolomics of Burkitt Lymphoma Mouse Models.
abstract: Burkitt lymphoma (BL) is a rare and highly aggressive type of non-Hodgkin lymphoma. The mortality rate of BL patients is very high due to the rapid growth rate and frequent systemic spread of the disease. A better understanding of the pathogenesis, more sensitive diagnostic tools and effective treatment methods for BL are essential. Metabolomics, an important aspect of systems biology, allows the comprehensive analysis of global, dynamic and endogenous biological metabolites based on their nuclear magnetic resonance (NMR) and mass spectrometry (MS). It has already been used to investigate the pathogenesis and discover new biomarkers for disease diagnosis and prognosis. In this study, we analyzed differences of serum metabolites in BL mice and normal mice by NMR-based metabolomics. We found that metabolites associated with energy metabolism, amino acid metabolism, fatty acid metabolism and choline phospholipid metabolism were altered in BL mice. The diagnostic potential of the metabolite differences was investigated in this study. Glutamate, glycerol and choline had a high diagnostic accuracy; in contrast, isoleucine, leucine, pyruvate, lysine, α-ketoglutarate, betaine, glycine, creatine, serine, lactate, tyrosine, phenylalanine, histidine and formate enabled the accurate differentiation of BL mice from normal mice. The discovery of abnormal metabolism and relevant differential metabolites may provide useful clues for developing novel, noninvasive approaches for the diagnosis and prognosis of BL based on these potential biomarkers.
authors.affiliation: National Laboratory for Molecular Sciences, Center for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, P. R. China.
authors.forename: Fengmin
authors.initials: F
authors.lastname: Yang
authors.affiliation: JOINN Laboratories, Inc., Beijing, P. R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Du
authors.affiliation: National Laboratory for Molecular Sciences, Center for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, P. R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Peking University People's Hospital and Institute of Hematology, Beijing, P. R. China.
authors.forename: Guorui
authors.initials: G
authors.lastname: Ruan
authors.affiliation: National Laboratory for Molecular Sciences, Center for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, P. R. China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Xiang
authors.affiliation: National Laboratory for Molecular Sciences, Center for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, P. R. China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Wang
authors.affiliation: National Laboratory for Molecular Sciences, Center for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, P. R. China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Sun
authors.affiliation: National Laboratory for Molecular Sciences, Center for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, P. R. China.
authors.forename: Aijiao
authors.initials: A
authors.lastname: Guan
authors.affiliation: National Laboratory for Molecular Sciences, Center for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, P. R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Shen
authors.affiliation: National Laboratory for Molecular Sciences, Center for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, P. R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: National Laboratory for Molecular Sciences, Center for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, P. R. China.
authors.forename: Xiaomeng
authors.initials: X
authors.lastname: Guo
authors.affiliation: National Laboratory for Molecular Sciences, Center for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, P. R. China.|University of the Chinese Academy of Sciences, Beijing, P. R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: National Laboratory for Molecular Sciences, Center for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, P. R. China.|University of the Chinese Academy of Sciences, Beijing, P. R. China.
authors.forename: Yalin
authors.initials: Y
authors.lastname: Tang
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood*; D002051:Burkitt Lymphoma / Q000097:blood* / Q000473:pathology; D004195:Disease Models, Animal; D004734:Energy Metabolism / Q000235:genetics; D005260:Female; D006801:Humans; D050356:Lipid Metabolism / Q000235:genetics; D008297:Male; D055432:Metabolomics; D051379:Mice
chemical_list: D014408:Biomarkers, Tumor