title: A Four-Well Dish for High-Resolution Longitudinal Imaging of the Tail and Posterior Trunk of Larval Zebrafish.
abstract: We describe the design, fabrication, and applications of a four-well dish for imaging of the trunk of larval zebrafish. The dish facilitates immobilization of anesthetized zebrafish larvae, with their tails gently pushed against a microscope cover glass, enabling longitudinal imaging at 24-72 h postfertilization using high-resolution objective lenses.
authors.affiliation: 1 Department of Cellular and Molecular Medicine, UC San Diego , La Jolla, California.
authors.forename: Bart
authors.initials: B
authors.lastname: Weijts
authors.affiliation: 2 School of Medicine, UC San Diego , La Jolla, California.
authors.forename: Eugene
authors.initials: E
authors.lastname: Tkachenko
authors.affiliation: 1 Department of Cellular and Molecular Medicine, UC San Diego , La Jolla, California.
authors.forename: David
authors.initials: D
authors.lastname: Traver
authors.affiliation: 3 Department of Physics, UC San Diego , La Jolla, California.
authors.forename: Alex
authors.initials: A
authors.lastname: Groisman
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D007091:Image Processing, Computer-Assisted; D007103:Immobilization / Q000295:instrumentation*; D007814:Larva / Q000033:anatomy & histology / Q000648:ultrastructure; D018613:Microscopy, Confocal / Q000379:methods*; D015027:Zebrafish / Q000033:anatomy & histology* / Q000254:growth & development*
keywords: imaging; vascular development; zebrafish