title: Noncovalent Bonding of RGD and YIGSR to an Electrospun Poly(ε-Caprolactone) Conduit through Peptide Self-Assembly to Synergistically Promote Sciatic Nerve Regeneration in Rats.
abstract: The nerve conduit with biofunctionalities can regulate neurite outgrowth, as well as the migration, proliferation, and myelination activity of Schwann cells. In the present study, polycaprolactone (PCL) conduits are coated with Naphthalene-phenylalanine-phenylalanine-glycine-arginine-glycine-aspartic (Nap-FFGRGD) and Naphthalene-phenylalanine-phenylalanine-glycine-cysteine-aspartic-proline-glycine-tyrosine-isoleucine-glycine-serine-arginine (Nap-FFGCDPGYIGSR) by self-assembly. In vitro studies demonstrate that arginine-glycine-aspartic (RGD) and tyrosine-isoleucine-glycine-serine-arginine (YIGSR) are capable of synergistically enhancing the ability of PCL to support the adhesion and proliferation of Schwann cells, as well as increasing neurite outgrowth from dorsal root ganglions explants. This synergistic effect may occur via the activation of both the phosphoinositide 3-kinase/protein kinase B and mitogen-activated protein kinase/extracellular signal-regulated protein kinase pathways. RGD/YIGSR modifications demonstrate beneficial effects across a 15 mm sciatic nerve gap in axonal regeneration and functional recovery. In addition, increased vascularization is observed in the RGD/YIGSR-PCL group, which might contribute to their beneficial effects on nerve regeneration. These findings indicate the potential of the RGD/YIGSR-PCL conduit to promote axonal regeneration and functional recovery, making the RGD/YIGSR-PCL conduit an attractive candidate for the treatment of a critical nerve defect.
authors.affiliation: Institute of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), College of Life Science, Nankai University, Tianjin, 300071, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: Institute of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, China.
authors.forename: Teng
authors.initials: T
authors.lastname: Ma
authors.affiliation: Institute of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, China.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Huang
authors.affiliation: Institute of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Xia
authors.affiliation: Institute of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Institute of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, China.
authors.forename: Yafeng
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Institute of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, China.
authors.forename: Zhongyang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Institute of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Quan
authors.affiliation: Institute of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), College of Life Science, Nankai University, Tianjin, 300071, China.
authors.forename: Deling
authors.initials: D
authors.lastname: Kong
authors.affiliation: Institute of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, China.
authors.forename: Jinghui
authors.initials: J
authors.lastname: Huang
authors.affiliation: Institute of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, China.
authors.forename: Zhuojing
authors.initials: Z
authors.lastname: Luo
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D009416:Nerve Regeneration / Q000187:drug effects* / Q000502:physiology; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000166:cytology / Q000378:metabolism; D012584:Sciatic Nerve / Q000502:physiology*
chemical_list: D020099:Coated Materials, Biocompatible; D009842:Oligopeptides; D011091:Polyesters; C081571:tyrosyl-isoleucyl-glycyl-seryl-arginine; C016240:polycaprolactone; C047981:arginyl-glycyl-aspartic acid; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt
keywords: axonal regeneration; peptides; peripheral nerve injury