title: Unlocking Cancer Glycomes from Histopathological Formalin-fixed and Paraffin-embedded (FFPE) Tissue Microdissections.
abstract: N- andO-glycans are attractive clinical biomarkers as glycosylation changes in response to diseases. The limited availability of defined clinical specimens impedes glyco-biomarker identification and validation in large patient cohorts. Formalin-fixed paraffin-embedded (FFPE) clinical specimens are the common form of sample preservation in clinical pathology, but qualitative and quantitativeN- andO-glycomics of such samples has not been feasible to date. Here, we report a highly sensitive and glycan isomer selective method for simultaneousN- andO-glycomics from histopathological slides. As few as 2000 cells isolated from FFPE tissue sections by laser capture microdissection were sufficient for in-depth histopathology-glycomics using porous graphitized carbon nanoLC ESI-MS/MS.N- andO-glycan profiles were similar between unstained and hematoxylin and eosin stained FFPE samples but differed slightly compared with fresh tissue. This method provides the key to unlock glyco-biomarker information from FFPE histopathological tissues archived in pathology laboratories worldwide.
authors.affiliation: From the ‡Max Planck Institute of Colloids and Interfaces, Department of Biomolecular Systems, 14424 Potsdam, Germany.|§Freie Universität Berlin, Department of Biology, Chemistry, Pharmacy, Institute of Chemistry and Biochemistry, 14195 Berlin, Germany.
authors.forename: Hannes
authors.initials: H
authors.lastname: Hinneburg
authors.affiliation: ¶Faculty of Science, Department of Biology, Division of Molecular Biology, University of Zagreb, Zagreb, Croatia.
authors.forename: Petra
authors.initials: P
authors.lastname: Korać
authors.affiliation: From the ‡Max Planck Institute of Colloids and Interfaces, Department of Biomolecular Systems, 14424 Potsdam, Germany.|§Freie Universität Berlin, Department of Biology, Chemistry, Pharmacy, Institute of Chemistry and Biochemistry, 14195 Berlin, Germany.
authors.forename: Falko
authors.initials: F
authors.lastname: Schirmeister
authors.affiliation: ‖Institute for Pathology and Cytology, University Hospital Merkur, Zagreb, Croatia.|**Department of Pathology, Medical School Zagreb, University of Zagreb, Zagreb, Croatia.
authors.forename: Slavko
authors.initials: S
authors.lastname: Gasparov
authors.affiliation: From the ‡Max Planck Institute of Colloids and Interfaces, Department of Biomolecular Systems, 14424 Potsdam, Germany.|§Freie Universität Berlin, Department of Biology, Chemistry, Pharmacy, Institute of Chemistry and Biochemistry, 14195 Berlin, Germany.
authors.forename: Peter H
authors.initials: PH
authors.lastname: Seeberger
authors.affiliation: ¶Faculty of Science, Department of Biology, Division of Molecular Biology, University of Zagreb, Zagreb, Croatia.
authors.forename: Vlatka
authors.initials: V
authors.lastname: Zoldoš
authors.affiliation: From the ‡Max Planck Institute of Colloids and Interfaces, Department of Biomolecular Systems, 14424 Potsdam, Germany; daniel.kolarich@mpikg.mpg.de.
authors.forename: Daniel
authors.identifier: 0000-0002-8452-1350
authors.initials: D
authors.lastname: Kolarich
mesh_terms: D015415:Biomarkers / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000379:methods; D054794:Glycomics / Q000379:methods*; D006801:Humans; D061346:Laser Capture Microdissection / Q000379:methods*; D009369:Neoplasms / Q000378:metabolism*; D016612:Paraffin Embedding; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods; D016707:Tissue Fixation
chemical_list: D015415:Biomarkers; D011134:Polysaccharides