title: Association of interleukin-25 levels with development of aspirin induced respiratory diseases.
abstract: Aspirin-exacerbated respiratory diseases (AERD) are caused by ingestion of non-steroidal anti-inflammatory drugs and are characterized by acute bronchospasms and marked infiltration of eosinophils, the latter being attributable to altered synthesis of cysteinyl leukotrienes (LT) and prostaglandins (PG). Recently, the innate Th2 response is revealed to induce eosinophil infiltration in allergic inflammation, however the role of the innate Th2 response has not been studies in AERD. Thus, we evaluated the relationship between the innate Th2 cytokines including IL-25, thymic stromal lymphopoietin (TSLP) and IL-33 and the development of AERD.
  
  
  
  Plasma IL-25, IL-33, and TSLP levels were measured before and after aspirin challenge in subjects with AERD (n = 25) and aspirin-tolerant asthma (ATA, n = 25) by enzyme-linked immunosorbent assay (ELISA). Pre and post-aspirin challenge levels of LTC4 and PGD2 were measured using ELISA.
  
  
  
  Basal plasma IL-25 levels were significantly higher in AERD group than in normal controls and in ATA group (p = 0.025 and 0.031, respectively). IL-33 and TSLP levels were comparable in the AERD and ATA groups. After the aspirin challenge, the IL-25 levels were markedly decreased in the ATA group (p = 0.024), while not changed in the AERD group. The post-challenge IL-25 levels of all asthmatic subjects were significantly correlated with aspirin challenge - induced declines in FEV1 (r = 0.357, p = 0.011), but not with basal and post challenge LTC4 and PGD2 levels.
  
  
  
  IL-25 is associated with bronchospasm after aspirin challenge, possibly via mechanisms other than altered LTC4 and PGD2 production.
authors.affiliation: Department of Interdisciplinary Program in Biomedical Science Major, Soonchunhyang Graduate School, Bucheon, Republic of Korea.
authors.forename: Jong-Uk
authors.initials: JU
authors.lastname: Lee
authors.affiliation: Department of Interdisciplinary Program in Biomedical Science Major, Soonchunhyang Graduate School, Bucheon, Republic of Korea; Genome Research Center, Division of Allergy and Respiratory Medicine, Soonchunhyang University, Bucheon Hospital, Republic of Korea.
authors.forename: Hun Soo
authors.initials: HS
authors.lastname: Chang
authors.affiliation: Department of Interdisciplinary Program in Biomedical Science Major, Soonchunhyang Graduate School, Bucheon, Republic of Korea.
authors.forename: Hyeon Ju
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Interdisciplinary Program in Biomedical Science Major, Soonchunhyang Graduate School, Bucheon, Republic of Korea.
authors.forename: Da-Jeong
authors.initials: DJ
authors.lastname: Bae
authors.affiliation: Department of Interdisciplinary Program in Biomedical Science Major, Soonchunhyang Graduate School, Bucheon, Republic of Korea.
authors.forename: Ji-Hye
authors.initials: JH
authors.lastname: Son
authors.affiliation: Genome Research Center, Division of Allergy and Respiratory Medicine, Soonchunhyang University, Bucheon Hospital, Republic of Korea.
authors.forename: Jong-Sook
authors.initials: JS
authors.lastname: Park
authors.affiliation: Division of Respiratory Medicine, Department of Internal Medicine, Soonchunhyang University, Cheonan Hospital, Republic of Korea.
authors.forename: Jae Sung
authors.initials: JS
authors.lastname: Choi
authors.affiliation: Division of Allergy and Respiratory Medicine, Department of Internal Medicine, Soonchunhyang University, Gumi Hospital, Republic of Korea.
authors.forename: Hun Gyu
authors.initials: HG
authors.lastname: Hwang
authors.affiliation: Department of Interdisciplinary Program in Biomedical Science Major, Soonchunhyang Graduate School, Bucheon, Republic of Korea; Genome Research Center, Division of Allergy and Respiratory Medicine, Soonchunhyang University, Bucheon Hospital, Republic of Korea. Electronic address: mdcspark@daum.net.
authors.forename: Choon-Sik
authors.initials: CS
authors.lastname: Park
mesh_terms: D000328:Adult; D000368:Aged; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology; D055963:Asthma, Aspirin-Induced / Q000097:blood / Q000276:immunology* / Q000503:physiopathology; D016207:Cytokines / Q000097:blood; D005260:Female; D005541:Forced Expiratory Volume / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects; D020381:Interleukin-17 / Q000097:blood*; D000067596:Interleukin-33 / Q000097:blood; D017997:Leukotriene C4 / Q000097:blood; D008297:Male; D008875:Middle Aged; D015230:Prostaglandin D2 / Q000097:blood; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D016207:Cytokines; C486701:IL25 protein, human; C506536:IL33 protein, human; D020381:Interleukin-17; D000067596:Interleukin-33; D017997:Leukotriene C4; D001241:Aspirin; D015230:Prostaglandin D2; D000094632:Thymic Stromal Lymphopoietin
keywords: Aspirin; Asthma; IL-25; Innate immune response