title: A novel Laccase Biosensor based on Laccase immobilized Graphene-Cellulose Microfiber Composite modified Screen-Printed Carbon Electrode for Sensitive Determination of Catechol.
abstract: In the present work, we demonstrate the fabrication of laccase biosensor to detect the catechol (CC) using laccase immobilized on graphene-cellulose microfibers (GR-CMF) composite modified screen printed carbon electrode (SPCE). The direct electrochemical behavior of laccase was investigated using laccase immobilized different modified SPCEs, such as GR/SPCE, CMF/SPCE and GR-CMF/SPCE. Compared with laccase immobilized GR and CMF modified SPCEs, a well-defined redox couple of CuI/CuIIfor laccase was observed at laccase immobilized GR-CMF composite modified SPCE. Cyclic voltammetry results show that the as-prepared biosensor has 7 folds higher catalytic activity with lower oxidation potential towards CC than SPCE modified with GR-CMF composite. Under optimized conditions, amperometric i-t method was used for the quantification of CC, and the amperometric response of the biosensor was linear over the concertation of CC ranging from 0.2 to 209.7 μM. The sensitivity, response time and the detection limit of the biosensor for CC is 0.932 μMμA-1cm-2, 2 s and 0.085 μM, respectively. The biosensor has high selectivity towards CC in the presence of potentially active biomolecules and phenolic compounds. The biosensor also accessed for the detection of CC in different water samples and shows good practicality with an appropriate repea.
authors.affiliation: Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei City, Taiwan, ROC.
authors.forename: Selvakumar
authors.initials: S
authors.lastname: Palanisamy
authors.affiliation: PG &Research department of Chemistry, Thiagarajar College, Madurai-09, Tamilnadu, India.
authors.forename: Sayee Kannan
authors.initials: SK
authors.lastname: Ramaraj
authors.affiliation: Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei City, Taiwan, ROC.
authors.forename: Shen-Ming
authors.initials: SM
authors.lastname: Chen
authors.affiliation: Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao East Road, Taipei City, Taiwan.
authors.forename: Thomas C K
authors.initials: TC
authors.lastname: Yang
authors.affiliation: Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei City, Taiwan, ROC.
authors.forename: Pan
authors.initials: P
authors.lastname: Yi-Fan
authors.affiliation: Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei City, Taiwan, ROC.
authors.forename: Tse-Wei
authors.initials: TW
authors.lastname: Chen
authors.affiliation: Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao East Road, Taipei City, Taiwan.|Division of Electrical and Electronic Engineering, School of Engineering, Manchester Metropolitan University, Manchester, M1 5GD, United Kingdom.
authors.forename: Vijayalakshmi
authors.initials: V
authors.lastname: Velusamy
authors.affiliation: PG &Research department of Chemistry, Thiagarajar College, Madurai-09, Tamilnadu, India.
authors.forename: Sonadevi
authors.initials: S
authors.lastname: Selvam
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D002396:Catechols / Q000032:analysis*; D002482:Cellulose / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D006108:Graphite / Q000737:chemistry; D042845:Laccase / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*
chemical_list: D002396:Catechols; D037742:Nanotubes, Carbon; D006108:Graphite; D002482:Cellulose; D042845:Laccase; C034221:catechol