title: Elevated expression of KLK8 predicts poor prognosis in colorectal cancer.
abstract: KLK8, also known as neuropsin, is one of fifteen members of the human kallikrein-related peptidase (KLK) gene family, which consists of enzymes with serine protease enzymatic activity. Aberrant KLK8 expression has been reported in several malignancies. However, the clinicopathological significance and prognostic value of KLK8 expression in colorectal cancer (CRC) are unknown. Therefore, analysis of public datasets, quantitative real-time PCR and western blot analysis were performed to assess KLK8 expression in CRC at both the mRNA and protein level. KLK8 expression was also assessed by immunohistochemistry in a tissue microarray containing 124 CRC specimens. We observed that KLK8 was overexpressed in CRC tissues and was significantly associated with TNM stage, vascular invasion, differentiation and AJCC stage. Univariate and multivariate Cox analyses confirmed that KLK8 is a significant independent prognostic factor for both DFS and OS. Cell function assays also indicated that KLK8 could facilitate CRC cell proliferation, migration and invasion in vitro. In conclusion, elevated KLK8 expression was correlated with the progression of CRC and is a potential independent prognostic indicator for CRC.
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Xuzhou Medical University, 32 Meijian Road, Xuzhou, 221006, Jiangsu, China.
authors.forename: Xianwu
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of General Surgery, XuZhou Central Hospital, 199 Jiefang Road, Xuzhou, 221009, Jiangsu, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Quan
authors.affiliation: Department of General Surgery, XuZhou Central Hospital, 199 Jiefang Road, Xuzhou, 221009, Jiangsu, China.
authors.forename: Zhilong
authors.initials: Z
authors.lastname: Tian
authors.affiliation: Department of General Surgery, XuZhou Central Hospital, 199 Jiefang Road, Xuzhou, 221009, Jiangsu, China.
authors.forename: Hailin
authors.initials: H
authors.lastname: Xi
authors.affiliation: Department of General Surgery, XuZhou Central Hospital, 199 Jiefang Road, Xuzhou, 221009, Jiangsu, China.
authors.forename: Gaolei
authors.initials: G
authors.lastname: Jia
authors.affiliation: Department of General Surgery, XuZhou Central Hospital, 199 Jiefang Road, Xuzhou, 221009, Jiangsu, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of General Surgery, XuZhou Central Hospital, 199 Jiefang Road, Xuzhou, 221009, Jiangsu, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, XuZhou Central Hospital, 199 Jiefang Road, Xuzhou, 221009, Jiangsu, China.
authors.forename: Ruming
authors.initials: R
authors.lastname: Liu
authors.affiliation: Department of General Surgery, XuZhou Central Hospital, 199 Jiefang Road, Xuzhou, 221009, Jiangsu, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Ma
authors.affiliation: Department of General Surgery, XuZhou Central Hospital, 199 Jiefang Road, Xuzhou, 221009, Jiangsu, China.
authors.forename: Fuzhou
authors.initials: F
authors.lastname: Han
authors.affiliation: Department of General Surgery, XuZhou Central Hospital, 199 Jiefang Road, Xuzhou, 221009, Jiangsu, China. Electronic address: lihuansongxzzx@163.com.
authors.forename: Huansong
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of General Surgery, XuZhou Central Hospital, 199 Jiefang Road, Xuzhou, 221009, Jiangsu, China. Electronic address: yuanfukangxzzx@163.com.
authors.forename: Fukang
authors.initials: F
authors.lastname: Yuan
mesh_terms: D000368:Aged; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D007610:Kallikreins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D011379:Prognosis; D016016:Proportional Hazards Models; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; C094799:KLK8 protein, human; D007610:Kallikreins
keywords: Colorectal cancer; KLK8; Prognosis; Tumorigenesis