title: Identification of a Novel Renal Coccidian (Apicomplexa: Eimeriidae) from the Great-Horned Owl ( Bubo virginianus ), USA.
abstract: We diagnosed renal coccidiosis in two of five Great-horned Owls ( Bubo virginianus ) examined in eastern Tennessee, US, 2007-13. Histopathologic examination of the kidneys revealed multifocal mild-to-moderate dilation and epithelial hyperplasia of collecting ducts. Renal collecting duct epithelial cells contained intracytoplasmic microgametocytes, macrogametocytes, and sporulating and sporulated oocysts. Renal coccidiosis in affected birds did not result in significant inflammation. Sequence analysis of the amplified partial 18S short subunit ribosomal RNA coding region from examination of formalin fixed tissue by using PCR disclosed a 93% identity to Eimeria reichenowi in GenBank, suggesting a novel Eimeria sp.
authors.affiliation: 1 Department of Biomedical and Diagnostic Sciences, College of Veterinary Medicine, University of Tennessee, 2407 River Drive, Knoxville, Tennessee 37996, USA.
authors.forename: Jennie M
authors.initials: JM
authors.lastname: Jankovsky
authors.affiliation: 1 Department of Biomedical and Diagnostic Sciences, College of Veterinary Medicine, University of Tennessee, 2407 River Drive, Knoxville, Tennessee 37996, USA.
authors.forename: Mabre
authors.initials: M
authors.lastname: Brand
authors.affiliation: 1 Department of Biomedical and Diagnostic Sciences, College of Veterinary Medicine, University of Tennessee, 2407 River Drive, Knoxville, Tennessee 37996, USA.
authors.forename: Richard W
authors.initials: RW
authors.lastname: Gerhold
mesh_terms: D000818:Animals; D003048:Coccidiosis / Q000662:veterinary*; D004539:Eimeria / Q000235:genetics* / Q000302:isolation & purification; D005243:Feces; D012337:RNA, Ribosomal, 18S; D020305:Strigiformes / Q000382:microbiology*; D013714:Tennessee
chemical_list: D012337:RNA, Ribosomal, 18S
keywords: 18s rDNA; Eimeria; Eimeria reichenowi; avian; coccidia; parasite; renal coccidiosis