title: ALK-negative anaplastic large cell lymphoma with urinary bladder involvement diagnosed in urine cytology: A case report and literature review.
abstract: Anaplastic large cell lymphoma is an aggressive T-cell neoplasm. It rarely involves the urinary bladder, with just twelve cases reported thus far and only one being ALK-negative. Immunophenotyping (particularly for ALK) is mandatory, both for prognostic and therapeutic reasons. Herein, we report the case of a patient with an ALK-negative anaplastic large cell lymphoma involving the bladder which was diagnosed and fully characterized by immunocytochemistry in urine cytology. The patient underwent a cystoscopy and the urine sample disclosed tumor diathesis background and aggregates of atypical cells, with evidence of multinucleation and mitotic figures. Immunocytochemistry revealed strong membrane/Golgi positivity for CD30 and negativity for ALK. The patient was submitted to transurethral resection for therapeutic purposes, which confirmed the diagnosis. To the best of our knowledge, this represents only the third case of anaplastic large cell lymphoma with bladder involvement diagnosed in urine cytology and the very first with diagnostic findings allowing for immunophenotyping of the disease in a bladder wash. The present report reinforces the role of urine cytology as a suitable method for establishing an earlier diagnosis and characterization of the disease, avoiding submitting patients to invasive procedures like transurethral resections. Diagn. Cytopathol. 2017;45:354-358. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Pathology, Portuguese Oncology Institute of Porto, Porto, Portugal.|Cancer Biology & Epigenetics Group - Research Center (CI-IPOP), Portuguese Oncology Institute of Porto, Portugal.|Department of Pathology and Molecular Immunology, Institute of Biomedical Sciences Abel Salazar - University of Porto (ICBAS-UP), Porto, Portugal.
authors.forename: João
authors.identifier: http://orcid.org/0000-0001-6829-1391
authors.initials: J
authors.lastname: Lobo
authors.affiliation: Department of Pathology, Portuguese Oncology Institute of Porto, Porto, Portugal.|Cancer Biology & Epigenetics Group - Research Center (CI-IPOP), Portuguese Oncology Institute of Porto, Portugal.|Department of Pathology and Molecular Immunology, Institute of Biomedical Sciences Abel Salazar - University of Porto (ICBAS-UP), Porto, Portugal.
authors.forename: Rui
authors.initials: R
authors.lastname: Henrique
authors.affiliation: Department of Pathology, Portuguese Oncology Institute of Porto, Porto, Portugal.|Cancer Biology & Epigenetics Group - Research Center (CI-IPOP), Portuguese Oncology Institute of Porto, Portugal.
authors.forename: Paula
authors.initials: P
authors.lastname: Monteiro
authors.affiliation: Department of Pathology, Portuguese Oncology Institute of Porto, Porto, Portugal.
authors.forename: Cláudia
authors.initials: C
authors.lastname: Lobo
mesh_terms: D000077548:Anaplastic Lymphoma Kinase; D006801:Humans; D017728:Lymphoma, Large-Cell, Anaplastic / Q000175:diagnosis* / Q000652:urine; D008297:Male; D008875:Middle Aged; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism; D001743:Urinary Bladder / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis* / Q000652:urine
chemical_list: C000626173:ALK protein, human; D000077548:Anaplastic Lymphoma Kinase; D020794:Receptor Protein-Tyrosine Kinases
keywords: ALK-1; anaplastic large cell lymphoma; bladder; cytology