title: Facile silicification of plastic surface for bioassays.
abstract: We herein report a biomimetic technique to modify plastic substrates for bioassays. The method first places a polydopamine adhesion layer to plastic surface, and then grows conformal silica coating. As proof of principle, we coated plastic microbeads to construct a disposable filter for point-of-care nucleic acid extraction.
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, Boston, MA 02114, USA. hlee@mgh.harvard.edu and Department of Radiology, Massachusetts General Hospital, Boston, MA 02114, USA.
authors.forename: Seonki
authors.identifier: http://orcid.org/0000-0003-1440-5167
authors.initials: S
authors.lastname: Hong
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, Boston, MA 02114, USA. hlee@mgh.harvard.edu and Department of Radiology, Massachusetts General Hospital, Boston, MA 02114, USA.
authors.forename: Ki Soo
authors.identifier: http://orcid.org/0000-0002-0545-0970
authors.initials: KS
authors.lastname: Park
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, Boston, MA 02114, USA. hlee@mgh.harvard.edu and Department of Radiology, Massachusetts General Hospital, Boston, MA 02114, USA and Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Ralph
authors.identifier: http://orcid.org/0000-0003-0828-4143
authors.initials: R
authors.lastname: Weissleder
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, Boston, MA 02114, USA. hlee@mgh.harvard.edu and Department of Medicine, Massachusetts General Hospital, Boston, MA 02114, USA. castro.cesar@mgh.harvard.edu.
authors.forename: Cesar M
authors.identifier: http://orcid.org/0000-0002-1159-5658
authors.initials: CM
authors.lastname: Castro
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, Boston, MA 02114, USA. hlee@mgh.harvard.edu and Department of Radiology, Massachusetts General Hospital, Boston, MA 02114, USA.
authors.forename: Hakho
authors.identifier: http://orcid.org/0000-0002-0087-0909
authors.initials: H
authors.lastname: Lee
mesh_terms: D032701:Biomimetics / Q000379:methods*; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005374:Filtration / Q000295:instrumentation*; D006801:Humans; D007211:Indoles / Q000737:chemistry*; D008863:Microspheres; D009696:Nucleic Acids / Q000302:isolation & purification*; D010969:Plastics / Q000737:chemistry*; D019095:Point-of-Care Systems; D011108:Polymers / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D007211:Indoles; D009696:Nucleic Acids; D010969:Plastics; D011108:Polymers; C568283:polydopamine; D012822:Silicon Dioxide