title: In vitro protective effects of botryosphaeran, a (1→3;1→6)-β-d-glucan, against mutagens in normal and tumor rodent cells.
abstract: Botryosphaeran (BOT) is an exocellular β-d-glucan (carbohydrate biopolymer) of the (1→3;1→6)-linked type produced by Botryosphaeria rhodina MAMB-05. The cytotoxic, mutagenic, genotoxic, and protective effects of this substance were evaluated in Chinese hamster lung fibroblasts (V79) and rat hepatocarcinoma cells (HTC) by the micronucleus test (MN) and the comet assay. BOT was not genotoxic in either cell line; it decreased the clastogenic effects of doxorubicin, H2O2, and benzo[a]pyrene. These results indicate that BOT may have potential as a therapeutic agent.
authors.affiliation: Departamento de Bioquímica e Biotecnologia, Centro de Ciências Exatas - Universidade Estadual de Londrina, CEP 86.057-970, Londrina, PR, Brazil. Electronic address: leakerche@gmail.com.
authors.forename: Leandra E
authors.initials: LE
authors.lastname: Kerche-Silva
authors.affiliation: Departamento de Biologia Geral, Centro de Ciências Biológicas - Universidade Estadual de Londrina, CEP 86.057-970, Londrina, PR, Brazil. Electronic address: ilcecolus@gmail.com.
authors.forename: Ilce M S
authors.initials: IM
authors.lastname: Cólus
authors.affiliation: Departamento de Biologia Geral, Centro de Ciências Biológicas - Universidade Estadual de Londrina, CEP 86.057-970, Londrina, PR, Brazil. Electronic address: maressa.malini@gmail.com.
authors.forename: Maressa
authors.initials: M
authors.lastname: Malini
authors.affiliation: Departamento de Biologia Geral, Centro de Ciências Biológicas - Universidade Estadual de Londrina, CEP 86.057-970, Londrina, PR, Brazil. Electronic address: mori_mateus@yahoo.com.br.
authors.forename: Mateus Prates
authors.initials: MP
authors.lastname: Mori
authors.affiliation: Programa de Pós-Graduação em Engenharia Ambiental, Universidade Tecnológica Federal do Paraná, Câmpus Londrina, CEP 86.036-370, Londrina, PR, Brazil. Electronic address: xylanase@gmail.com.
authors.forename: Robert F H
authors.initials: RF
authors.lastname: Dekker
authors.affiliation: Departamento de Bioquímica e Biotecnologia, Centro de Ciências Exatas - Universidade Estadual de Londrina, CEP 86.057-970, Londrina, PR, Brazil. Electronic address: anelibarbosa@gmail.com.
authors.forename: Aneli M
authors.initials: AM
authors.lastname: Barbosa-Dekker
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D006224:Cricetinae; D003412:Cricetulus; D005936:Glucans / Q000494:pharmacology*; D066298:In Vitro Techniques; D009152:Mutagenicity Tests; D051381:Rats
chemical_list: D016587:Antimutagenic Agents; D005936:Glucans; C483238:botryosphaeran
keywords: Benzo[a]pyrene; Chemoprevention; Doxorubicin; HTC cells; Hydrogen peroxide; V79 cells