title: Microbiome Remodeling via the Montmorillonite Adsorption-Excretion Axis Prevents Obesity-related Metabolic Disorders.
abstract: Obesity and its related metabolic disorders are closely correlated with gut dysbiosis. Montmorillonite is a common medicine used to treat diarrhea. We have previously found that dietary lipid adsorbent-montmorillonite (DLA-M) has an unexpected role in preventing obesity. The aim of this study was to further investigate whether DLA-M regulates intestinal absorption and gut microbiota to prevent obesity-related metabolic disorders. Here, we show that DLA-M absorbs free fatty acids (FFA) and endotoxins in vitro and in vivo. Moreover, the combination of fluorescent tracer technique and polarized light microscopy showed that DLA-M crystals immobilized BODIPY® FL C16 and FITC-LPS, respectively, in the digestive tract in situ. HFD-fed mice treated with DLA-M showed mild changes in the composition of the gut microbiota, particularly increases in short-chain fatty acids (SCFA)-producing Blautia bacteria and decreases in endotoxin-producing Desulfovibrio bacteria, these changes were positively correlated with obesity and inflammation. Our results indicated that DLA-M immobilizes FFA and endotoxins in the digestive tract via the adsorption-excretion axis and DLA-M may potentially be used as a prebiotic to prevent intestinal dysbiosis and obesity-associated metabolic disorders in obese individuals.
authors.affiliation: Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Xu
authors.affiliation: Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Hong
authors.affiliation: Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China.
authors.forename: Jialin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Aging Research, School of Medicine, Hangzhou Normal University, Hangzhou 311121, China.
authors.forename: Yusheng
authors.initials: Y
authors.lastname: Cong
authors.affiliation: Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Dai
authors.affiliation: Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Biology Science and Technology, Baotou Teacher's College, Baotou 014030, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China; Key Laboratory for Cell Proliferation and Regulation Biology of State Education Ministry, College of Life Sciences, Beijing Normal University, Beijing 100875, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Jin
authors.affiliation: Department of Medical Genetics and Cell Biology, School of Basic Sciences, Guangzhou Medical University, Guangzhou 511436, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China; Key Laboratory for Cell Proliferation and Regulation Biology of State Education Ministry, College of Life Sciences, Beijing Normal University, Beijing 100875, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.affiliation: Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China; Key Laboratory for Cell Proliferation and Regulation Biology of State Education Ministry, College of Life Sciences, Beijing Normal University, Beijing 100875, China. Electronic address: ygzhai@bnu.edu.cn.
authors.forename: Yonggong
authors.initials: Y
authors.lastname: Zhai
mesh_terms: D000818:Animals; D000931:Antidotes / Q000494:pharmacology; D001546:Bentonite / Q000494:pharmacology*; D015153:Blotting, Western; D018955:CD36 Antigens / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D059305:Diet, High-Fat / Q000009:adverse effects; D004731:Endotoxins / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005234:Fatty Liver / Q000503:physiopathology / Q000517:prevention & control; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000517:prevention & control; D007408:Intestinal Absorption / Q000187:drug effects*; D008297:Male; D008659:Metabolic Diseases / Q000503:physiopathology / Q000517:prevention & control*; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D064307:Microbiota / Q000187:drug effects* / Q000502:physiology; D009765:Obesity / Q000209:etiology / Q000503:physiopathology / Q000517:prevention & control*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000931:Antidotes; D018955:CD36 Antigens; D004731:Endotoxins; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D001546:Bentonite
keywords: Endotoxins; Free fatty acids; Gut microbiota; Metabolic disorders; Montmorillonite; Obesity