title: Synthetic polyphosphate inhibits endogenous coagulation and platelet aggregationin vitro.
abstract: Platelet-derived polyphosphate has previously been indicated to induce coagulation. However, industrially synthesized polyphosphate has been found to have different effects from those of the platelet-derived form. The present study investigated whether synthetic sodium polyphosphate inhibits coagulation using routine coagulation tests and thromboelastography. Synthetic polyphosphate was found to inhibit adenosine diphosphate-, epinephrine-, arachidonic acid-, ristocetin-, thrombin-, oxytocin- and pituitrin-induced platelet aggregation. The effects of synthetic polyphosphate in clotting inhibition were revealed by the analysis of clotting factor activity and platelet aggregation tests. Synthetic polyphosphate may inhibit platelet aggregation by reducing platelet calcium levels, as indicated by the results of flow cytometric analysis and high-throughput fluorescent screening. Furthermore, analysis of thromboxane (TX)B2 by ELISA indicated that synthetic polyphosphate reduces platelet aggregation by inhibiting the TXA2 signaling pathway. In conclusion, synthetic polyphosphate inhibits clotting factor activity and endogenous coagulation by reducing the levels of calcium ions and TXA2 to curb platelet aggregation.
authors.affiliation: Department of Hematology, Haikou Municipal Hospital, Affiliated Haikou Hospital of Central South University, Haikou, Hainan 570208, P.R. China; Department of Hematology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Hematology, Haikou Municipal Hospital, Affiliated Haikou Hospital of Central South University, Haikou, Hainan 570208, P.R. China.
authors.forename: Mengjie
authors.initials: M
authors.lastname: Wan
authors.affiliation: Department of Hematology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Ting
authors.initials: T
authors.lastname: Liang
authors.affiliation: Department of Hematology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Minyuan
authors.initials: M
authors.lastname: Peng
authors.affiliation: Department of Hematology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China; Department of Hematology, The Third Hospital of Xiangya Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Fangping
authors.initials: F
authors.lastname: Chen
keywords: high-throughput screening system; platelet aggregation; polyphosphate; routine coagulation test; thromboelastogram