title: Hepatocellular and developmental influences of early postnatal indomethacin in mice.
abstract: The effect of early postnatal indomethacin exposure on hepatocellular and developmental alterations in mice liver was investigated.
  
  
  
  Pups received IP injections of 0, 25, 50 and 100 mg/kg indomethacin on P0, then killed at P21 and P60.
  
  
  
  Indomethacin significantly suppressed body weight at P21, but liver weight significantly decreased only in 25 mg/kg. In contrast, liver weight and liver to body weight ratio significantly increased with increasing dose of indomethacin by P60. The restoration of liver weight was a result of proliferation, as a consequence of a significant increase in the number of uni and bi-nuclear hepatocytes per field in 25 mg/kg at P21 and no evidence of hepatocellular hypertrophy. Indomethacin had a dose-related decrease in number of hepatocytes as the result of hepatocellular hypertrophy confirmed with hepatocytes presenting large cellular and nuclear size in 50 and 100 mg/kg. Moreover, proliferation contributed to the increased liver size, since bi-nuclear hepatocytes and its ratio increased at P21 at first and then decreased by P60 with increasing in dose.
  
  
  
  Indomethacin has long term effect on liver development in a dose- and time- dependent manner. The hepatocytes during both the liver development and regeneration show significant differences in cell and nuclear number and size (Tab. 3, Fig. 2, Ref. 48).
authors.forename: F
authors.initials: F
authors.lastname: Nowrouzi
authors.forename: M
authors.initials: M
authors.lastname: Azadbakht
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001835:Body Weight / Q000187:drug effects*; D002467:Cell Nucleus / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D022781:Hepatocytes / Q000187:drug effects*; D007213:Indomethacin / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000254:growth & development*; D008115:Liver Regeneration / Q000187:drug effects; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009929:Organ Size / Q000187:drug effects
chemical_list: D007213:Indomethacin
keywords: development alterations mice.; hepatocellular alterations; indomethacin; liver