title: Do the complementarities of electrokinetic and chromatographic procedures represent the "Swiss knife" in proteomic investigation? An overview of the literature in the past decade.
abstract: This report reviews the literature of the past decade dealing with the combination of electrokinetic and chromatographic strategies in the proteomic field. Aim of this article is to highlight how the application of complementary techniques may contribute to substantially improve protein identification. Several studies here considered demonstrate that exploring the combination of these approaches can be a strategy to enrich the extent of proteomic information achieved from a sample. The coupling of "top-down" and "bottom-up" proteomics may result in the generation of a hybrid analytical tool, very efficient not only for large-scale profiling of complex proteomes but also for studying specific subproteomes. The range of applications described, while evidencing a continuous boost in the imagination of researchers for developing new combinations of methods for protein separation, also underlines the adaptability of these techniques to a wide variety of samples. This report points out the general usefulness of combining different procedures for proteomic analysis, an approach that allows researchers to go deeper in the proteome of samples under investigation.
authors.affiliation: Department of Molecular Medicine, Biochemistry Unit, University of Pavia, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Di Venere
authors.affiliation: Department of Molecular Medicine, Biochemistry Unit, University of Pavia, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Viglio
authors.affiliation: Department of Biology and Biotechnologies "L. Spallanzani,", Biochemistry Unit, University of Pavia, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Sassera
authors.affiliation: Department of Biology and Biotechnologies "L. Spallanzani,", Biochemistry Unit, University of Pavia, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Fumagalli
authors.affiliation: Department of Molecular Medicine, Biochemistry Unit, University of Pavia, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Bardoni
authors.affiliation: Department of Molecular Medicine, Biochemistry Unit, University of Pavia, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Salvini
authors.affiliation: Department of Molecular Medicine, Biochemistry Unit, University of Pavia, Italy.
authors.forename: Maddalena
authors.initials: M
authors.lastname: Cagnone
authors.affiliation: Department of Biology and Biotechnologies "L. Spallanzani,", Biochemistry Unit, University of Pavia, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Iadarola
mesh_terms: D000818:Animals; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary / Q000379:methods*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D011506:Proteins
keywords: 1DE; 2DE; CE-MS; Hybrid technologies; LC-MS