title: Cortactin and phosphorylated cortactin tyr466expression in temporal bone carcinoma.
abstract: OBJECTIVE
  Cortactin is a multidomain protein engaged in several cellular mechanisms involving actin assembly and cytoskeletal arrangement. Cortactin overexpression in several malignancies has been associated with increased cell migration, invasion, and metastatic potential. Cortactin needs to be activated by tyrosine or serine/threonine phosphorylation. The role of cortactin and phosphorylated cortactin (residue tyr466) was investigated in temporal bone squamous cell carcinoma (TBSCC).
  
  
  METHODS
  Immunohistochemical expression of cortactin and phosphorylated cortactin (residue tyr466) was assessed in 27 consecutively-operated TBSCCs.
  
  
  RESULTS
  Several clinicopathological variables correlated with recurrence (pT stage, dura mater involvement), and disease-free survival (DFS) (cT stage, pT stage, pN status, dura mater involvement). Twenty-three of 24 immunohistochemically evaluable TBSCCs were cortactin-positive. Median cortactin expression was 75.0%. Cortactin reaction in the cytoplasm was more intense in carcinoma cells than in normal adjacent tissue. Recurrence and DFS rates did not correlate with cortactin and phosphorylated cortactin (residue tyr466) expression in TBSCC specimens.
  
  
  CONCLUSIONS
  Cortactin upregulation in TBSCC supports the conviction that inhibiting cortactin functions could have selective effects on this malignancy. Multi-institutional studies should further investigate the role of cortactin and phosphorylated cortactin in TBSCC, and their potential clinical application in integrated treatment modalities.
authors.affiliation: Department of Neurosciences DNS, Otolaryngology Section, Padova University, Padova, Italy. Electronic address: gino.marioni@unipd.it.
authors.forename: Gino
authors.initials: G
authors.lastname: Marioni
authors.affiliation: Department of Neurosciences DNS, Otolaryngology Section, Padova University, Padova, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Zanoletti
authors.affiliation: Department of Neurosciences DNS, Otolaryngology Section, Padova University, Padova, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Mazzoni
authors.affiliation: Anatomic Pathology Unit, Ospedali Riuniti Bergamo, Bergamo, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Gianatti
authors.affiliation: Department of Medicine DIMED, University of Padova, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Valentini
authors.affiliation: Department of Neurosciences DNS, Otolaryngology Section, Padova University, Padova, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Girasoli
authors.affiliation: Department of Neurosciences DNS, Otolaryngology Section, Padova University, Padova, Italy.
authors.forename: Martina
authors.initials: M
authors.lastname: Guariento
authors.affiliation: Department of Medicine DIMED, University of Padova, Italy.
authors.forename: Luciano
authors.initials: L
authors.lastname: Giacomelli
authors.affiliation: Department of Neurosciences DNS, Otolaryngology Section, Padova University, Padova, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Martini
authors.affiliation: Department of Medicine DIMED, University of Padova, Italy.
authors.forename: Stella
authors.initials: S
authors.lastname: Blandamura
mesh_terms: D001859:Bone Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D051356:Cortactin / Q000378:metabolism*; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D010766:Phosphorylation; D011379:Prognosis; D013701:Temporal Bone / Q000378:metabolism* / Q000601:surgery; D015854:Up-Regulation
chemical_list: D051356:Cortactin
keywords: Cortactin; Phosphorylated; Prognosis; Target therapy; Temporal bone squamous cell carcinoma