title: Chitosan coatings with enhanced biostability in vivo.
abstract: In this article, we study the stability of chitosan coatings applied on glutaraldehyde-stabilized bovine pericardium when exposed to biodegradation in vivo in the course of model subcutaneous tests on rats. The coatings were deposited from carbonic acid solutions, that is, H2O saturated with CO2at high pressure. Histological sections of treated pericardium samples demonstrated that the structure of pericardial connective tissues was not significantly altered by the coating application method. It was revealed that the dynamics of biodegradation depended on the total mass of chitosan applied as well as on the DDA of chitosan used. As long as the amount of chitosan did not exceed a certain threshold limit, no detectable degradation occurred within the time of the tests (12 weeks for the rat model). For higher chitosan amounts, we detected a ∼20% reduction of the mass after the in vivo exposition. The presumed mechanism of such behavior is discussed. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 270-277, 2018.
authors.affiliation: Faculty of Physics, Lomonosov Moscow State University, Leninskie gory 1-2, Moscow, 119991, Russian Federation.|Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Vavilova 28, Moscow, 119991, Russian Federation.
authors.forename: Marat O
authors.identifier: http://orcid.org/0000-0001-7739-3074
authors.initials: MO
authors.lastname: Gallyamov
authors.affiliation: Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Vavilova 28, Moscow, 119991, Russian Federation.
authors.forename: Ivan S
authors.initials: IS
authors.lastname: Chaschin
authors.affiliation: Faculty of Physics, Lomonosov Moscow State University, Leninskie gory 1-2, Moscow, 119991, Russian Federation.
authors.forename: Matvey V
authors.initials: MV
authors.lastname: Bulat
authors.affiliation: Bakulev Scientific Center for Cardiovascular Surgery of the Ministry of Health of the Russian Federation, Roublyevskoe Sh. 135, Moscow, 121552, Russian Federation.
authors.forename: Natalia P
authors.initials: NP
authors.lastname: Bakuleva
authors.affiliation: Faculty of Chemistry, Lomonosov Moscow State University, Leninskie gory 1-2, Moscow, 119991, Russian Federation.
authors.forename: Gennadii A
authors.initials: GA
authors.lastname: Badun
authors.affiliation: Faculty of Chemistry, Lomonosov Moscow State University, Leninskie gory 1-2, Moscow, 119991, Russian Federation.
authors.forename: Maria G
authors.initials: MG
authors.lastname: Chernysheva
authors.affiliation: Faculty of Physics, Lomonosov Moscow State University, Leninskie gory 1-2, Moscow, 119991, Russian Federation.
authors.forename: Olga I
authors.initials: OI
authors.lastname: Kiselyova
authors.affiliation: Faculty of Physics, Lomonosov Moscow State University, Leninskie gory 1-2, Moscow, 119991, Russian Federation.|Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Vavilova 28, Moscow, 119991, Russian Federation.
authors.forename: Alexei R
authors.initials: AR
authors.lastname: Khokhlov
mesh_terms: D000818:Animals; D002417:Cattle; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology; D008422:Materials Testing; D051381:Rats
chemical_list: D020099:Coated Materials, Biocompatible; D003094:Collagen; D048271:Chitosan
keywords: biodegradation; bovine pericardium; carbonic acid; chitosan coating; heart valve bioprosthesis