title: BASIC PENTACYSTEINE Proteins Repress ABSCISIC ACID INSENSITIVE4 Expression via Direct Recruitment of the Polycomb-Repressive Complex 2 in Arabidopsis Root Development.
abstract: Plant transcription factors generally act in complex regulatory networks that function at multiple levels to govern plant developmental programs. Dissection of the interconnections among different classes of transcription factors can elucidate these regulatory networks and thus improve our understanding of plant development. Here, we investigated the molecular and functional relationships of the transcription factors ABSCISIC ACID INSENSITIVE 4 (ABI4) and members of the BASIC PENTACYSTEINE (BPC) family in lateral root (LR) development of Arabidopsis thaliana. Genetic analysis showed that BPCs promote LR development by repressing ABI4 expression. Molecular analysis showed that BPCs bind to the ABI4 promoter and repress ABI4 transcription in roots. BPCs directly recruit the Polycomb Repressive Complex 2 (PRC2) to the ABI4 locus and epigenetically repress ABI4 expression by catalyzing the trimethylation of histone H3 at Lys27. In addition, BPCs and ABI4 co-ordinate their activities to fine-tune the levels of PIN-FORMED1, a component of the auxin signaling pathway, and thus modulate LR formation. These results establish a functional relationship between two universal and multiple-role transcription factors, and provide insight into the mechanisms of the transcriptional regulatory networks that affect Arabidopsis organogenesis.
authors.affiliation: Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Mu
authors.affiliation: Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China.
authors.forename: Meijuan
authors.initials: M
authors.lastname: Zou
authors.affiliation: Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China.
authors.forename: Xuwu
authors.initials: X
authors.lastname: Sun
authors.affiliation: Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Baoye
authors.initials: B
authors.lastname: He
authors.affiliation: Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Xu
authors.affiliation: Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China.
authors.forename: Yini
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chi
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D008745:Methylation; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism*; D063151:Polycomb Repressive Complex 2; D011485:Protein Binding; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C470421:ABI4 protein, Arabidopsis; D029681:Arabidopsis Proteins; C574170:PRC2 protein, Arabidopsis; D012097:Repressor Proteins; D014157:Transcription Factors; D063151:Polycomb Repressive Complex 2
keywords: ABI4; Arabidopsis; BPC; LR development; PRC2