title: Overexpression of a chrysanthemum transcription factor gene DgNAC1 improves the salinity tolerance in chrysanthemum.
abstract: DgNAC1, a transcription factor of chrysanthemum, was functionally verified to confer salt stress responses by regulating stress-responsive genes. NAC transcription factors play effective roles in resistance to different abiotic stresses, and overexpressions of NAC TFs in Arabidopsis have been proved to be conducive in improving salinity tolerance. However, functions of NAC genes in chrysanthemum continue to be poorly understood. Here, we performed physiology and molecular experiments to evaluate roles of DgNAC1 in chrysanthemum salt stress responses. In this study, DgNAC1-overexpressed chrysanthemum was obviously more resistant to salt over the WT (wild type). Specifically, the transgenic chrysanthemum showed a higher survival rate and lower EC (electrolyte conductivity) than WT under salt stress. The transgenic chrysanthemum also showed fewer accumulations of MDA (malondialdehyde) and reactive oxygen species (H2O2and O2-), greater activities of SOD (superoxide dismutase), POD (peroxidase) and CAT (catalase), as well as more proline content than WT under salt stress. Furthermore, stress-responsive genes in transgenic chrysanthemum were greater up-regulated than in WT under salinity stress. Thus, all results revealed that DgNAC1 worked as a positive regulator in responses to salt stress and it may be an essential gene for molecular breeding of salt-tolerant plants.
authors.affiliation: Department of Ornamental Horticulture, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, 611130, Sichuan, People's Republic of China.
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Ornamental Horticulture, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, 611130, Sichuan, People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhong
authors.affiliation: Department of Ornamental Horticulture, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, 611130, Sichuan, People's Republic of China.
authors.forename: Yin-Huan
authors.initials: YH
authors.lastname: Wu
authors.affiliation: Department of Ornamental Horticulture, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, 611130, Sichuan, People's Republic of China.
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Bai
authors.affiliation: Department of Ornamental Horticulture, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, 611130, Sichuan, People's Republic of China.
authors.forename: Qian-Yu
authors.initials: QY
authors.lastname: Liang
authors.affiliation: Department of Ornamental Horticulture, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, 611130, Sichuan, People's Republic of China. qinglinliu@126.com.
authors.forename: Qing-Lin
authors.initials: QL
authors.lastname: Liu
authors.affiliation: Department of Ornamental Horticulture, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, 611130, Sichuan, People's Republic of China.
authors.forename: Yuan-Zhi
authors.initials: YZ
authors.lastname: Pan
authors.affiliation: Department of Ornamental Horticulture, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, 611130, Sichuan, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Ornamental Horticulture, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, 611130, Sichuan, People's Republic of China.
authors.forename: Bei-Bei
authors.initials: BB
authors.lastname: Jiang
authors.affiliation: Department of Ornamental Horticulture, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, 611130, Sichuan, People's Republic of China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Department of Ornamental Horticulture, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, 611130, Sichuan, People's Republic of China.
authors.forename: Guang-Li
authors.initials: GL
authors.lastname: Liu
mesh_terms: D031202:Chrysanthemum / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D006861:Hydrogen Peroxide / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D054712:Salinity; D055049:Salt Tolerance / Q000235:genetics*; D055051:Salt-Tolerant Plants / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors; D012965:Sodium Chloride; D006861:Hydrogen Peroxide
keywords: Chrysanthemum; DgNAC1; ROS-scavenging systems; Salt stress; Transcription factor