title: Dermal CD271+ Cells are Closely Associated with Regeneration of the Dermis in the Wound Healing Process.
abstract: Stem cells have recently been shown to play important roles in wound healing. The aim of this study was to investigate the role of dermal CD271+ cells in wound healing. Full-thickness wounds were produced on the backs of 5-year-old and 24-week-old mice, and time-course of wound closure, CD271+ cell counts, and gene expression levels were compared. Delayed wound healing was observed in 24-week-old mice. The peak of CD271+ cell increase was delayed in 24-week-old mice, and gene expression levels of growth factors in wounded tissue were significantly increased in 5-year-old mice. Dermal CD271+ cells purified by fluorescence-activated cell sorting (FACS) expressed higher growth factors than CD271- cells, suggesting that CD271+ cells play important roles by producing growth factors. This study also investigated dermal CD271+ cells in patients with chronic skin ulcers. Dermal CD271+ cells in patients were significantly reduced compared with in healthy controls. Thus, dermal CD271+ cells are closely associated with wound healing.
authors.affiliation: Department of Dermatology, Fujita Health University School of Medicine, 1-98 Kutsukake-cho, Toyoake, Aichi 470-1192, Japan. iwayou@fujita-hu.ac.jp.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Iwata
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Hasebe
authors.forename: Seiji
authors.initials: S
authors.lastname: Hasegawa
authors.forename: Satoru
authors.initials: S
authors.lastname: Nakata
authors.forename: Akiko
authors.initials: A
authors.lastname: Yagami
authors.forename: Kayoko
authors.initials: K
authors.lastname: Matsunaga
authors.forename: Kazumitsu
authors.initials: K
authors.lastname: Sugiura
authors.forename: Hirohiko
authors.initials: H
authors.lastname: Akamatsu
mesh_terms: D000068816:Adapalene / Q000276:immunology* / Q000378:metabolism; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000276:immunology / Q000473:pathology; D000818:Animals; D016022:Case-Control Studies; D049109:Cell Proliferation; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000276:immunology*; D010641:Phenotype; D017475:Receptors, Nerve Growth Factor / Q000276:immunology*; D012867:Skin / Q000276:immunology* / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D012883:Skin Ulcer / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D013234:Stem Cells / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D014945:Wound Healing; D014950:Wounds, Penetrating / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000401:mortality
chemical_list: C484065:NGFR protein, human; D009419:Nerve Tissue Proteins; D017475:Receptors, Nerve Growth Factor; D000068816:Adapalene