title: Enteroviruses infect human enteroids and induce antiviral signaling in a cell lineage-specific manner.
abstract: Enteroviruses are among the most common viral infectious agents of humans and are primarily transmitted by the fecal-oral route. However, the events associated with enterovirus infections of the human gastrointestinal tract remain largely unknown. Here, we used stem cell-derived enteroids from human small intestines to study enterovirus infections of the intestinal epithelium. We found that enteroids were susceptible to infection by diverse enteroviruses, including echovirus 11 (E11), coxsackievirus B (CVB), and enterovirus 71 (EV71), and that contrary to an immortalized intestinal cell line, enteroids induced antiviral and inflammatory signaling pathways in response to infection in a virus-specific manner. Furthermore, using the Notch inhibitor dibenzazepine (DBZ) to drive cellular differentiation into secretory cell lineages, we show that although goblet cells resist E11 infection, enteroendocrine cells are permissive, suggesting that enteroviruses infect specific cell populations in the human intestine. Taken together, our studies provide insights into enterovirus infections of the human intestine, which could lead to the identification of novel therapeutic targets and/or strategies to prevent or treat infections by these highly clinically relevant viruses.
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219.
authors.forename: Coyne G
authors.initials: CG
authors.lastname: Drummond
authors.affiliation: Division of Newborn Medicine, Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15224.
authors.forename: Alexa M
authors.initials: AM
authors.lastname: Bolock
authors.affiliation: Division of Newborn Medicine, Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15224.
authors.forename: Congrong
authors.initials: C
authors.lastname: Ma
authors.affiliation: Division of Newborn Medicine, Department of Pediatrics, Washington University School of Medicine, St. Louis, MO 63110.
authors.forename: Cliff J
authors.initials: CJ
authors.lastname: Luke
authors.affiliation: Division of Newborn Medicine, Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15224; coynec2@pitt.edu mgood@kids.wustl.edu.
authors.forename: Misty
authors.initials: M
authors.lastname: Good
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219; coynec2@pitt.edu mgood@kids.wustl.edu.
authors.forename: Carolyn B
authors.initials: CB
authors.lastname: Coyne
mesh_terms: D018938:Caco-2 Cells; D019070:Cell Lineage / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D003984:Dibenzazepines / Q000494:pharmacology; D060467:Disease Resistance / Q000235:genetics; D004770:Enterovirus / Q000502:physiology*; D004769:Enterovirus Infections / Q000378:metabolism / Q000821:virology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D020869:Gene Expression Profiling / Q000379:methods; D054884:Host-Pathogen Interactions; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism / Q000821:virology; D007421:Intestine, Small / Q000166:cytology / Q000378:metabolism / Q000821:virology*; D009940:Organoids / Q000166:cytology / Q000378:metabolism / Q000821:virology*; D015398:Signal Transduction / Q000235:genetics
chemical_list: D003984:Dibenzazepines; D004791:Enzyme Inhibitors; C527817:dibenzazepine
keywords: enteroendocrine cells; enteroid; enterovirus; goblet cells; innate immune