title: Ruthenium(II) Complex Incorporated UiO-67 Metal-Organic Framework Nanoparticles for Enhanced Two-Photon Fluorescence Imaging and Photodynamic Cancer Therapy.
abstract: Ruthenium(II) tris(bipyridyl) cationic complex (Ru(bpy)32+) incorporated UiO-67 (Universitetet i Oslo) nanoscale metal-organic frameworks (NMOFs) with an average diameter of ∼92 nm were developed as theranostic nanoplatform for in vitro two-photon fluorescence imaging and photodynamic therapy. After incorporation into porous UiO-67 nanoparticles, the quantum yield, luminescence lifetime, and two-photon fluorescence intensity of Ru(bpy)32+guest molecules were much improved owing to the steric confinement effect of MOF pores. Benefiting from these merits, the as-synthesized nanoparticles managed to be internalized by A549 cells while providing excellent red fluorescence in cytoplasm upon excitation with 880 nm irradiation. Photodynamic therapeutic application of the Ru(bpy)32+-incorporated UiO-67 NMOFs was investigated in vitro. The Ru(bpy)32+-incorporated UiO-67 NMOFs exhibited good biocompatibility without irradiation while having good cell-killing rates upon irradiation. In view of these facts, the developed Ru(bpy)32+-incorporated NMOFs give a new potential pathway to achieve enhanced two-photon fluorescence imaging and photodynamic therapy.
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Zhang
authors.forename: Jipsa
authors.initials: J
authors.lastname: Chelora
authors.forename: Yuan
authors.initials: Y
authors.lastname: Xiong
authors.forename: Stephen V
authors.identifier: http://orcid.org/0000-0003-0408-4902
authors.initials: SV
authors.lastname: Kershaw
authors.affiliation: Department of Physics and Institute of Advanced Materials, Hong Kong Baptist University , Kowloon Tong, Hong Kong SAR 999077, P. R. China.
authors.forename: King Fai
authors.initials: KF
authors.lastname: Li
authors.forename: Pik-Kwan
authors.identifier: http://orcid.org/0000-0001-5255-1718
authors.initials: PK
authors.lastname: Lo
authors.affiliation: Department of Physics and Institute of Advanced Materials, Hong Kong Baptist University , Kowloon Tong, Hong Kong SAR 999077, P. R. China.
authors.forename: Kok Wai
authors.initials: KW
authors.lastname: Cheah
authors.forename: Andrey L
authors.identifier: http://orcid.org/0000-0002-8263-8141
authors.initials: AL
authors.lastname: Rogach
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: Zapien
authors.forename: Chun-Sing
authors.identifier: http://orcid.org/0000-0001-6557-453X
authors.initials: CS
authors.lastname: Lee
mesh_terms: D005453:Fluorescence; D006801:Humans; D053768:Metal Nanoparticles; D000073396:Metal-Organic Frameworks; D009369:Neoplasms; D012428:Ruthenium
chemical_list: D000073396:Metal-Organic Frameworks; D012428:Ruthenium
keywords: metal−organic frameworks; nanoscale metal−organic frameworks; photodynamic therapy; theranostics; two-photon excited fluorescence