title: Zebrafish phosvitin-derived peptide Pt5 inhibits melanogenesis via cAMP pathway.
abstract: Zebrafish phosvitin-derived peptide Pt5, consisting of the C-terminal 55 residues of phosvitin, has been shown to have an antimicrobial-immunomodulatory activity comparable to phosvitin. Here, we showed clearly that Pt5 had the capacity to inhibit tyrosinase (TYR) activity and melanin biosynthesis, and this inhibition was independent of cell proliferation and cytotoxic effects. Incubation of fluorescein isothiocyanate (FITC)-labeled Pt5 with B16F10 melanoma cells revealed that Pt5 was localized in the cytoplasm of the cells. In addition, Pt5 inhibited the expression of TYR, tyrosinase-related protein-1 (TRP-1), tyrosinase-related protein-2 (TRP-2), and microphthalmia-associated transcription factor (MITF) in B16F10 melanoma cells and reduced the intracellular cyclic adenosine monophosphate (cAMP) concentration in the cells, but it did not affect the cellular contents of pERK1/2 and β-catenin, suggesting that Pt5 regulates melanin biosynthesis via cAMP signaling pathway rather than Wnt and MAPK pathways. Collectively, these data indicate that Pt5 has the potential to be used as a melanogenesis inhibitor in medical and cosmetic industry, a novel role ever reported.
authors.affiliation: Department of Medical Laboratory, Shaoyang University, Shaoyang, Hunan, 422000, China.|Laboratory for Evolution & Development, Institute of Evolution & Marine Biodiversity, Department of Marine Biology, Ocean University of China, Room 205, Ke Xue Guan, 5 Yushan Road, Qingdao, 266003, China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Liu
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo, Zhejiang, 315211, China.
authors.forename: Xiu-Rong
authors.initials: XR
authors.lastname: Su
authors.affiliation: Laboratory for Evolution & Development, Institute of Evolution & Marine Biodiversity, Department of Marine Biology, Ocean University of China, Room 205, Ke Xue Guan, 5 Yushan Road, Qingdao, 266003, China.
authors.forename: Shou-Sheng
authors.initials: SS
authors.lastname: Liu
authors.affiliation: Laboratory for Evolution & Development, Institute of Evolution & Marine Biodiversity, Department of Marine Biology, Ocean University of China, Room 205, Ke Xue Guan, 5 Yushan Road, Qingdao, 266003, China.
authors.forename: Shuang-Shuang
authors.initials: SS
authors.lastname: Yang
authors.affiliation: Laboratory for Evolution & Development, Institute of Evolution & Marine Biodiversity, Department of Marine Biology, Ocean University of China, Room 205, Ke Xue Guan, 5 Yushan Road, Qingdao, 266003, China.|College of Life Science and Technology, Honghe University, Mengzi, Yunnan, 661100, China.
authors.forename: Cheng-Yan
authors.initials: CY
authors.lastname: Jiang
authors.affiliation: Laboratory for Evolution & Development, Institute of Evolution & Marine Biodiversity, Department of Marine Biology, Ocean University of China, Room 205, Ke Xue Guan, 5 Yushan Road, Qingdao, 266003, China. yuzhang@ouc.edu.cn.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Laboratory for Evolution & Development, Institute of Evolution & Marine Biodiversity, Department of Marine Biology, Ocean University of China, Room 205, Ke Xue Guan, 5 Yushan Road, Qingdao, 266003, China. sczhang@ouc.edu.cn.
authors.forename: Shicui
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D000242:Cyclic AMP / Q000378:metabolism*; D019746:Intramolecular Oxidoreductases / Q000378:metabolism; D008543:Melanins / Q000096:biosynthesis*; D051379:Mice; D014442:Monophenol Monooxygenase / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D010446:Peptide Fragments / Q000494:pharmacology*; D010774:Phosvitin / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D029961:Zebrafish Proteins / Q000494:pharmacology*
chemical_list: D008543:Melanins; D010446:Peptide Fragments; C000588961:Pt5 peptide, zebrafish; D011994:Recombinant Proteins; D029961:Zebrafish Proteins; D010774:Phosvitin; D000242:Cyclic AMP; D010088:Oxidoreductases; C064056:tyrosinase-related protein-1; D014442:Monophenol Monooxygenase; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase
keywords: Melanogenesis; Phosvitin-derived peptide; Tyrosinase; Yolk protein; Zebrafish