title: Expression Analysis of Lily Type Lectin Isotypes in the Rock Bream,Oplegnathus fasciatus: in the Tissue, Developmental Stage and Viral Infection.
abstract: Lectins belong to the pattern-recognition receptors (PRRs) class and play important roles in the recognition and elimination of pathogens via the innate immune system. Recently, it was reported that lily-type lectin-1 is involved when a pathogen attacks in the early immune response of fish. However, this study is limited to information that the lectin is involved in the innate immune response against viral infection. In the present study, the lily-type lectin-2 and -3 ofOplegnathus fasciatus(OfLTL-2 and 3) have been presented to be included B-lectin domain and two D-mannose binding sites in the amino acid sequence that an important feature for the fundamental structure. To investigate the functional properties of OfLTLs, the tissue distribution in the healthy rock bream and temporal expression during early developmental stage analysis are performed using quantitative real-time PCR. OfLTL-2 and 3 are predominantly expressed in the liver and skin, but rarely expressed in other organ. Also, the transcripts of OfLTLs are not expressed during the early developmental stage but its transcripts are increased after immune-related organs which are fully formed. In the challenge experiment with RBIV (rock bream iridovirus), the expression of OfLTLs was increased much more strongly in the late response than the early, unlike previously known. These results suggest that OfLTLs are specifically expressed in the immune-related tissues when those organs are fully formed and it can be inferred that the more intensively involved in the second half to the virus infection.
authors.affiliation: Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS), Geoje 53334, Republic of Korea.
authors.forename: Young Mee
authors.initials: YM
authors.lastname: Lee
authors.affiliation: Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS), Geoje 53334, Republic of Korea.
authors.forename: In Jung
authors.initials: IJ
authors.lastname: Yang
authors.affiliation: Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS), Geoje 53334, Republic of Korea.
authors.forename: Jae Koo
authors.initials: JK
authors.lastname: Noh
authors.affiliation: Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS), Geoje 53334, Republic of Korea.
authors.forename: Hyun Chul
authors.initials: HC
authors.lastname: Kim
authors.affiliation: Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS), Geoje 53334, Republic of Korea.
authors.forename: Choul-Ji
authors.initials: CJ
authors.lastname: Park
authors.affiliation: Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS), Geoje 53334, Republic of Korea.
authors.forename: Jong-Won
authors.initials: JW
authors.lastname: Park
authors.affiliation: Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS), Geoje 53334, Republic of Korea.
authors.forename: Gyeong Eon
authors.initials: GE
authors.lastname: Noh
authors.affiliation: Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS), Geoje 53334, Republic of Korea.
authors.forename: Woo-Jin
authors.initials: WJ
authors.lastname: Kim
authors.affiliation: Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS), Geoje 53334, Republic of Korea.
authors.forename: Kyung-Kil
authors.initials: KK
authors.lastname: Kim
keywords: Gene expression; Immune response; Lily type lectin; Oplegnathus fasciatus; Rock bream; Rock bream iridovirus (RBIV)