title: Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary.
abstract: Biotherapeutic proteins, such as monoclonal antibodies (mAbs), are feasible alternatives for the treatment of chronic-degenerative diseases. The biological activity of these proteins depends on their physicochemical properties. The use of high-performance techniques like chromatography and capillary electrophoresis has been described for the analysis of physicochemical heterogeneity of mAbs. Nowadays, capillary zone electrophoresis (CZE) technique constitutes one of the most resolutive and sensitive assays for the analysis of biomolecules. Besides, the electro-driven separation in CZE is governed by extensive properties of matter and offers the advantage of analyzing proteins close to their native state. However, the successful implementation of this technique for routine analysis depends on the skills of the analyst at the critical steps during sample and system preparation. The purpose of this tutorial is to detail the steps to succeed in the CZE analysis of mAbs. Further, this protocol can be used for the development and improvement of skills of the personnel involved in protein analytical chemistry laboratories.
authors.affiliation: Unidad de Investigación y Desarrollo, Probiomed S.A. de C.V.
authors.forename: Carlos Eduardo
authors.initials: CE
authors.lastname: Espinosa-de la Garza
authors.affiliation: Unidad de Investigación y Desarrollo, Probiomed S.A. de C.V.
authors.forename: Rodolfo Daniel
authors.initials: RD
authors.lastname: Salazar-Flores
authors.affiliation: Unidad de Investigación y Desarrollo, Probiomed S.A. de C.V.
authors.forename: Néstor Octavio
authors.initials: NO
authors.lastname: Pérez
authors.affiliation: Unidad de Investigación y Desarrollo, Probiomed S.A. de C.V.; luis.flores@probiomed.com.mx.
authors.forename: Luis Francisco
authors.initials: LF
authors.lastname: Flores-Ortiz
authors.affiliation: Unidad de Investigación y Desarrollo, Probiomed S.A. de C.V.; emrivero@yahoo.com.mx.
authors.forename: Emilio
authors.initials: E
authors.lastname: Medina-Rivero
mesh_terms: D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D020033:Protein Isoforms / Q000302:isolation & purification
chemical_list: D000911:Antibodies, Monoclonal; D020033:Protein Isoforms