title: Wheat Bran Does Not Affect Postprandial Plasma Short-Chain Fatty Acids from13C-inulin Fermentation in Healthy Subjects.
abstract: Wheat bran (WB) is a constituent of whole grain products with beneficial effects for human health. Within the human colon, such insoluble particles may be colonized by specific microbial teams which can stimulate cross-feeding, leading to a more efficient carbohydrate fermentation and an increased butyrate production. We investigated the extent to which WB fractions with different properties affect the fermentation of other carbohydrates in the colon. Ten healthy subjects performed four test days, during which they consumed a standard breakfast supplemented with 10 g13C-inulin. A total of 20 g of a WB fraction (unmodified WB, wheat bran with a reduced particle size (WB RPS), or de-starched pericarp-enriched wheat bran (PE WB)) was also added to the breakfast, except for one test day, which served as a control. Blood samples were collected at regular time points for 14 h, in order to measure13C-labeled short-chain fatty acid (SCFA; acetate, propionate and butyrate) concentrations. Fermentation of13C-inulin resulted in increased plasma SCFA for about 8 h, suggesting that a sustained increase in plasma SCFA can be achieved by administering a moderate dose of carbohydrates, three times per day. However, the addition of a single dose of a WB fraction did not further increase the13C-SCFA concentrations in plasma, nor did it stimulate cross-feeding (Wilcoxon signed ranks test).
authors.affiliation: Translational Research in Gastrointestinal Disorders, KU Leuven, Leuven 3000, Belgium. lise.deroover@kuleuven.be.
authors.forename: Lise
authors.initials: L
authors.lastname: Deroover
authors.affiliation: Centre for Food and Microbial Technology, KU Leuven, Leuven 3000, Belgium. joran.verspreet@kuleuven.be.|Leuven Food Science and Nutrition Research Centre, KU Leuven, Leuven 3000, Belgium. joran.verspreet@kuleuven.be.
authors.forename: Joran
authors.initials: J
authors.lastname: Verspreet
authors.affiliation: Translational Research in Gastrointestinal Disorders, KU Leuven, Leuven 3000, Belgium. anja.luypaerts@kuleuven.be.
authors.forename: Anja
authors.initials: A
authors.lastname: Luypaerts
authors.affiliation: Translational Research in Gastrointestinal Disorders, KU Leuven, Leuven 3000, Belgium. greet.vandermeulen@kuleuven.be.
authors.forename: Greet
authors.initials: G
authors.lastname: Vandermeulen
authors.affiliation: Centre for Food and Microbial Technology, KU Leuven, Leuven 3000, Belgium. christophe.courtin@kuleuven.be.|Leuven Food Science and Nutrition Research Centre, KU Leuven, Leuven 3000, Belgium. christophe.courtin@kuleuven.be.
authors.forename: Christophe M
authors.initials: CM
authors.lastname: Courtin
authors.affiliation: Translational Research in Gastrointestinal Disorders, KU Leuven, Leuven 3000, Belgium. kristin.verbeke@kuleuven.be.|Leuven Food Science and Nutrition Research Centre, KU Leuven, Leuven 3000, Belgium. kristin.verbeke@kuleuven.be.
authors.forename: Kristin
authors.initials: K
authors.lastname: Verbeke
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D062408:Breakfast; D002247:Carbon Isotopes; D018592:Cross-Over Studies; D004043:Dietary Fiber / Q000378:metabolism / Q000627:therapeutic use*; D064806:Dysbiosis / Q000097:blood / Q000378:metabolism / Q000382:microbiology / Q000517:prevention & control*; D005232:Fatty Acids, Volatile / Q000097:blood / Q000378:metabolism*; D005260:Female; D005285:Fermentation; D000069196:Gastrointestinal Microbiome; D006801:Humans; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000382:microbiology; D007444:Inulin / Q000378:metabolism*; D008297:Male; D010316:Particle Size; D019518:Postprandial Period; D056692:Prebiotics; D015203:Reproducibility of Results; D016037:Single-Blind Method; D055815:Young Adult
chemical_list: D015415:Biomarkers; D002247:Carbon Isotopes; D004043:Dietary Fiber; D005232:Fatty Acids, Volatile; D056692:Prebiotics; D007444:Inulin
keywords: colonic fermentation; inulin; short-chain fatty acids; wheat bran