title: The Level of Serum and Urinary Nephrin in Normal Pregnancy and Pregnancy with Subsequent Preeclampsia.
abstract: OBJECTIVE
  The aim of this study was to evaluate serum and urinary nephrin levels of normal pregnancy to establish a standard reference value and to compare them with patients who subsequently developed preeclampsia (PE).
  
  
  METHODS
  In this prospective study, 117 healthy singleton pregnancies were enrolled between 6 to 20 weeks of gestation at 2 participating medical centers during October 2010 to March 2012. Urine and serum samples were collected at the time of enrollment, each trimester, and at 4 to 6 weeks postpartum. Enzyme-linked immunosorbent assay for nephrin was performed and samples from patients who subsequently developed PE were compared to the normal patients.
  
  
  RESULTS
  Of 117 patients initially enrolled, 99 patients delivered at the study centers and of those patients, 12 (12.1%) developed PE at a median gestational age of 34⁺⁴ weeks (range 29⁺⁵-36⁺⁶). In the normal patients (n=68), serum nephrin level decreased and urinary nephrin level increased during the latter of pregnancy. In 12 patients who subsequently developed PE, a significant rise in the 3rd trimester serum and urinary nephrin levels, compared to the controls, was observed (p<0.001), and this increase occurred 9 days prior to the onset of clinical disease.
  
  
  CONCLUSIONS
  As the onset of PE was preceded by the rise in the serum and urinary nephrin in comparison to normal pregnancy, serum and urinary nephrin may be a useful predictive marker of PE.
authors.affiliation: Department of Obstetrics and Gynecology, Institute of Women's Life Medical Science, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Yun Ji
authors.initials: YJ
authors.lastname: Jung
authors.affiliation: Department of Obstetrics and Gynecology, Institute of Women's Life Medical Science, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Hee Young
authors.initials: HY
authors.lastname: Cho
authors.affiliation: Department of Obstetrics and Gynecology, Institute of Women's Life Medical Science, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Sihyun
authors.initials: S
authors.lastname: Cho
authors.affiliation: Department of Obstetrics and Gynecology, Institute of Women's Life Medical Science, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Young Han
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Department of Obstetrics and Gynecology, MizMedi Women's Hospital, Seoul, Korea.
authors.forename: Jin Dong
authors.initials: JD
authors.lastname: Jeon
authors.affiliation: Department of Bioanalysis, Seoul Medical Science Institute & Seoul Clinical Laboratories, Seoul, Korea.
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Department of Bioanalysis, Seoul Medical Science Institute & Seoul Clinical Laboratories, Seoul, Korea.
authors.forename: Sanghoo
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Bioanalysis, Seoul Medical Science Institute & Seoul Clinical Laboratories, Seoul, Korea.
authors.forename: Jimyeong
authors.initials: J
authors.lastname: Park
authors.affiliation: Biostatistics Collaboration Unit, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Ha Yan
authors.initials: HY
authors.lastname: Kim
authors.affiliation: Department of Obstetrics and Gynecology, Institute of Women's Life Medical Science, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Yong Won
authors.initials: YW
authors.lastname: Park
authors.affiliation: Department of Obstetrics and Gynecology, Institute of Women's Life Medical Science, Yonsei University College of Medicine, Seoul, Korea. jaykwon@yuhs.ac.
authors.forename: Ja Young
authors.initials: JY
authors.lastname: Kwon
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood / Q000652:urine; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005865:Gestational Age; D006801:Humans; D008565:Membrane Proteins / Q000097:blood* / Q000652:urine*; D011225:Pre-Eclampsia / Q000097:blood / Q000209:etiology* / Q000652:urine; D011247:Pregnancy; D011257:Pregnancy Proteins; D011263:Pregnancy Trimester, Third / Q000097:blood / Q000652:urine; D011446:Prospective Studies; D012015:Reference Standards
chemical_list: D015415:Biomarkers; D008565:Membrane Proteins; D011257:Pregnancy Proteins; C113267:nephrin
keywords: Nephrin; preeclampsia; serum; urine