title: Spontaneous Activity Characteristics of 3D "Optonets".
abstract: Sporadic spontaneous network activity emerges during early central nervous system (CNS) development and, as the number of neuronal connections rises, the maturing network displays diverse and complex activity, including various types of synchronized patterns. These activity patterns have major implications on both basic research and the study of neurological disorders, and their interplay with network morphology tightly correlates with developmental events such as neuronal differentiation, migration and establishment of neurotransmitter phenotypes. Although 2D neural cultures models have provided important insights into network activity patterns, these cultures fail to mimic the complex 3D architecture of natural CNS neural networks and its consequences on connectivity and activity. A 3Din-vitromodel mimicking early network development while enabling cellular-resolution observations, could thus significantly advance our understanding of the activity characteristics in the developing CNS. Here, we longitudinally studied the spontaneous activity patterns of developing 3Din-vitroneural network "optonets," an optically-accessible bioengineered CNS model with multiple cortex-like characteristics. Optonet activity was observed using the genetically encodable calcium indicator GCaMP6m and a 3D imaging solution based on a standard epi-fluorescence microscope equipped with a piezo-electric z-stage and image processing-based deconvolution. Our results show that activity patterns become more complex as the network matures, gradually exhibiting longer-duration events. This report characterizes the patterns over time, and discusses how environmental changes affect the activity patterns. The relatively high degree of similarity between the network's spontaneously generated activity patterns and the reported characteristics ofin-vivoactivity, suggests that this is a compelling model system for brain-in-a chip research.
authors.affiliation: Department of Biomedical Engineering, Technion - Israel Institute of Technology Haifa, Israel.
authors.forename: Anat
authors.initials: A
authors.lastname: Marom
authors.affiliation: Department of Biomedical Engineering, Technion - Israel Institute of Technology Haifa, Israel.
authors.forename: Erez
authors.initials: E
authors.lastname: Shor
authors.affiliation: Department of Biomedical Engineering, Technion - Israel Institute of Technology Haifa, Israel.
authors.forename: Shulamit
authors.initials: S
authors.lastname: Levenberg
authors.affiliation: Department of Biomedical Engineering, Technion - Israel Institute of Technology Haifa, Israel.
authors.forename: Shy
authors.initials: S
authors.lastname: Shoham
keywords: 3D cultures; Neural activity; Neural development; calcium imaging