title: Transient loading of CD34+hematopoietic progenitor cells with polystyrene nanoparticles.
abstract: CD34+hematopoietic progenitor cells (HPCs) offer great opportunities to develop new treatments for numerous malignant and non-malignant diseases. Nanoparticle (NP)-based strategies can further enhance this potential, and therefore a thorough understanding of the loading behavior of HPCs towards NPs is essential for a successful application. The present study focusses on the interaction kinetics of 40 nm sized carboxylated polystyrene (PS) NPs with HPCs. Interestingly, a transient association of the NPs with HPCs is observed, reaching a maximum within 1 hour and declining afterwards. This behavior is not seen in dendritic cells (CD34-DCs) differentiated from HPCs, which display a monotonic increase in NP load. We demonstrate that this transient interaction requires an energy-dependent cellular process, suggesting active loading and release of NPs by HPCs. This novel observation offers a unique approach to transiently equip HPCs. A simple theoretical approach modeling the kinetics of NP loading and release is presented, contributing to a framework of describing this phenomenon.
authors.affiliation: VITO, Flemish Institute for Technological Research, Mol, Belgium; Biomedical Research Institute, Hasselt University, Diepenbeek, Belgium.
authors.forename: Sarah
authors.initials: S
authors.lastname: Deville
authors.affiliation: VITO, Flemish Institute for Technological Research, Mol, Belgium.
authors.forename: Wahyu Wijaya
authors.initials: WW
authors.lastname: Hadiwikarta
authors.affiliation: VITO, Flemish Institute for Technological Research, Mol, Belgium; Biomedical Research Institute, Hasselt University, Diepenbeek, Belgium.
authors.forename: Nick
authors.initials: N
authors.lastname: Smisdom
authors.affiliation: VITO, Flemish Institute for Technological Research, Mol, Belgium; Theoretical Physics, Hasselt University, Diepenbeek, Belgium.
authors.forename: Bart
authors.initials: B
authors.lastname: Wathiong
authors.affiliation: Biomedical Research Institute, Hasselt University, Diepenbeek, Belgium.
authors.forename: Marcel
authors.initials: M
authors.lastname: Ameloot
authors.affiliation: VITO, Flemish Institute for Technological Research, Mol, Belgium.
authors.forename: Inge
authors.initials: I
authors.lastname: Nelissen
authors.affiliation: VITO, Flemish Institute for Technological Research, Mol, Belgium; Theoretical Physics, Hasselt University, Diepenbeek, Belgium.
authors.forename: Jef
authors.initials: J
authors.lastname: Hooyberghs
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry; D016923:Cell Death; D049109:Cell Proliferation; D002478:Cells, Cultured; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D007700:Kinetics; D008164:Luminescent Proteins / Q000737:chemistry; D008954:Models, Biological; D053758:Nanoparticles / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*
chemical_list: D018952:Antigens, CD34; D001426:Bacterial Proteins; D008164:Luminescent Proteins; D011137:Polystyrenes; C054562:yellow fluorescent protein, Bacteria; D049452:Green Fluorescent Proteins
keywords: dendritic cells; hematopoietic progenitor cells; nanoparticles; release; uptake