title: CRISPR-Cas-Induced Mutants Identify a Requirement for dSTIM in Larval Dopaminergic Cells ofDrosophila melanogaster.
abstract: Molecular components of store-operated calcium entry have been identified in the recent past and consist of the endoplasmic reticulum (ER) membrane-resident calcium sensor STIM and the plasma membrane-localized calcium channel Orai. The physiological function of STIM and Orai is best defined in vertebrate immune cells. However, genetic studies with RNAi strains inDrosophilasuggest a role in neuronal development and function. We generated a CRISPR-Cas-mediated deletion for the gene encoding STIM inDrosophila(dSTIM), which we demonstrate is larval lethal. To study STIM function in neurons, we merged the CRISPR-Cas9 method with the UAS-GAL4 system to generate either tissue- or cell type-specific inducible STIM knockouts (KOs). Our data identify an essential role for STIM in larval dopaminergic cells. The molecular basis for this cell-specific requirement needs further investigation.
authors.affiliation: National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, Karnataka 560065, India.|Manipal University, Karnataka 576104, India.
authors.forename: Trayambak
authors.initials: T
authors.lastname: Pathak
authors.affiliation: National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, Karnataka 560065, India.
authors.forename: Deepti
authors.initials: D
authors.lastname: Trivedi
authors.affiliation: National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, Karnataka 560065, India gaiti@ncbs.res.in.
authors.forename: Gaiti
authors.identifier: 0000-0001-7194-383X
authors.initials: G
authors.lastname: Hasan
mesh_terms: D000818:Animals; D049628:Body Size; D064113:CRISPR-Cas Systems / Q000235:genetics*; D059290:Dopaminergic Neurons / Q000166:cytology* / Q000378:metabolism*; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000166:cytology / Q000235:genetics*; D055786:Gene Knockout Techniques; D006720:Homozygote; D007814:Larva / Q000378:metabolism; D009154:Mutation / Q000235:genetics*; D009474:Neurons / Q000378:metabolism; D009928:Organ Specificity / Q000235:genetics
chemical_list: D029721:Drosophila Proteins
keywords: Orai; SOCE; hypoderm; null allele; tyrosine hydroxylase