title: Effect of storage conditions on the LDL effectiveness in ovine sperm cryopreservation.
abstract: The present study evaluated the effect of storage conditions on the LDL efficacy for cryopreserving ovine sperm. In this way, we compared egg yolk extender with three different forms for LDL storing, LDL diluted in Tris-glucose extender and stored in frozen (i) and freeze-dried (ii) states and LDL stored pure and added into the extender prior to use (iii). We also tested the effect of two storage temperatures (-20 and -80 °C) and three storage times (30, 60, 120 d). Frozen and freeze-dried extenders containing LDL, as well as LDL stored pure, improved post-thaw sperm quality. Storage temperatures did not influence negatively the cryoprotectiveness of LDL extenders. Furthermore, lyophilised LDL extenders stored at -20 °C were more effective in preserving sperm longevity than the other extenders stored at -20 °C. Finally, LDL extenders stored for 30 and 120 d were more efficient than 60 d in preserving ram sperm freezability.
authors.affiliation: Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna, Km 16, Salobrinho, Ilhéus, BA 45650-000, Brazil. Electronic address: paolasnoeck@uesc.br.
authors.forename: P P N
authors.initials: PPN
authors.lastname: Snoeck
authors.affiliation: Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna, Km 16, Salobrinho, Ilhéus, BA 45650-000, Brazil.
authors.forename: L C O
authors.initials: LCO
authors.lastname: Moura
authors.affiliation: Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna, Km 16, Salobrinho, Ilhéus, BA 45650-000, Brazil.
authors.forename: M C
authors.initials: MC
authors.lastname: Silva
authors.affiliation: Departamento de Biologia Geral, Universidade Federal de Viçosa, Av. Peter Henry Rolfs s/n, Campus Universitário, Viçosa, MG 36570-000, Brazil. Electronic address: mariana.mneves@ufv.br.
authors.forename: M
authors.initials: M
authors.lastname: Machado-Neves
authors.affiliation: Departamento de Medicina Veterinária, Pontifícia Universidade Católica de Minas Gerais, Unidade Betim, Rua do Rosário, 1081, Angola, Betim, MG 32604-115, Brazil. Electronic address: bel.melo@terra.com.br.
authors.forename: M I V
authors.initials: MIV
authors.lastname: Melo
authors.affiliation: Laboratório de Imunologia, Fundação Ezequiel Dias - FUNED, Rua Conde Pereira Carneiro 80, Gameleira, Belo Horizonte, MG 30510-010, Brazil. Electronic address: heneinel@gmail.com.
authors.forename: L G D
authors.initials: LGD
authors.lastname: Heneine
authors.affiliation: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Rua Leonil Prata 819, Alípio de Melo, Belo Horizonte, MG 30840780, Brazil. Electronic address: henrym2601@gmail.com.
authors.forename: M
authors.initials: M
authors.lastname: Henry
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005612:Freeze Drying; D005615:Freezing; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008297:Male; D012662:Semen Preservation / Q000379:methods*; D012756:Sheep; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects; D013696:Temperature
chemical_list: D003451:Cryoprotective Agents; D008077:Lipoproteins, LDL
keywords: Cryopreservation; Low-density lipoproteins; Lyophilisation; Ovine; Semen; Storage period