title: Up-regulation of plakophilin-2 is correlated with the progression of glioma.
abstract: Glioma is the most common type of primary brain tumor in the CNS. Due to its poor prognosis and high mortality rates, it is urgent to find out more effective therapies. Plakophilin-2 (PKP2) is a widespread desmosomal plaque protein. Recently, the important roles of PKP2 in the proliferation and migration of cancer cells and tumor progression has been shown. However, the expression and potential function of PKP2 in glioma was still unclear. In this study, we demonstrated that PKP2 protein expression level was increased in glioma tissues compared with normal brain tissues, and its level was significantly associated with the Ki-67 expression and WHO grade by Western blot analysis and immunohistochemistry. Clinically, high PKP2 expression was tightly related to poor prognosis of glioma patients. Interestingly, we found that down-regulated PKP2 expression was shown to inhibit the migration of cells in glioma. Moreover, cell counting kit (CCK)-8 and colony formation analyses proved that reduced expression of PKP2 could weaken glioma cell proliferation. Taken together, these data uncover a potential role for PKP2 in the pathogenic process of glioma, suggesting that PKP2 may be a promising therapeutic target of glioma.
authors.affiliation: Department of Oncology, Taizhou People's Hospital, Taizhou, China.
authors.forename: Degeng
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Hematology, Taizhou People's Hospital, Taizhou, China.
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Qian
authors.affiliation: Department of Oncology, Taizhou People's Hospital, Taizhou, China.
authors.forename: Xiaoxing
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Pathology, Taizhou People's Hospital, Taizhou, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Neurology, Taizhou People's Hospital, Taizhou, China.
authors.forename: Niangao
authors.initials: N
authors.lastname: Zhao
authors.affiliation: Department of Hematology, Taizhou People's Hospital, Taizhou, China.
authors.forename: Zhengdong
authors.initials: Z
authors.lastname: Wu
mesh_terms: D000328:Adult; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D018450:Disease Progression; D015536:Down-Regulation; D005260:Female; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D053208:Kaplan-Meier Estimate; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D051191:Plakophilins / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D019394:Ki-67 Antigen; C495337:PKP2 protein, human; D051191:Plakophilins
keywords: PKP2; glioma; migration; proliferation; up-regulation