title: Coagulation Management in Jersey Calves: An ex vivo Study.
abstract: OBJECTIVE
  Jersey calves are frequently used as an experimental animal model for in vivo testing of cardiac assist devices or orthopedic implants. In this ex vivo study, we analyzed the coagulation system of the Jersey calves and the potential of human-based coagulation management to circumvent perioperative bleeding complications during surgery. Experimental Procedure: Blood from 7 Jersey calves was subjected to standard laboratory tests and thromboelastometry analysis. An ex vivo model of dilutional coagulopathy was used to study the effects of fibrinogen or prothrombin complex concentrate supplementation. Fibrinolysis was induced with tissue plasminogen activator to identify potential therapeutic strategies involving tranexamic acid or aprotinin. Furthermore, anticoagulation strategies were evaluated by incubating the blood samples with dabigatran or rivaroxaban.
  
  
  RESULTS
  Baseline values for thromboelastometry and standard laboratory parameters, including prothrombin time, activated partial thromboplastin time, fibrinogen, antithrombin III, and D-dimers, were established. Fifty percent diluted blood showed a statistically significant impairment of hemostasis. The parameters significantly improved after the administration of fibrinogen or prothrombin complex concentrate. Tranexamic acid and aprotinin ameliorated tissue plasminogen activator-induced fibrinolysis. Both dabigatran and rivaroxaban significantly prolonged the coagulation parameters.
  
  
  CONCLUSIONS
  In this ex vivo study, coagulation factors, factor concentrate, antifibrinolytic reagents, and anticoagulants regularly used in the clinic positively impacted coagulation parameters in Jersey calf blood.
authors.affiliation: Department of Anaesthesiology, RWTH Aachen University Hospital, Aachen, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Gröning
authors.forename: Judith
authors.initials: J
authors.lastname: Maas
authors.forename: Svenja
authors.initials: S
authors.lastname: van Geul
authors.forename: Rolf
authors.initials: R
authors.lastname: Rossaint
authors.forename: Ulrich
authors.initials: U
authors.lastname: Steinseifer
authors.forename: Oliver
authors.initials: O
authors.lastname: Grottke
mesh_terms: D000818:Animals; D000925:Anticoagulants; D000933:Antifibrinolytic Agents; D001777:Blood Coagulation; D001780:Blood Coagulation Tests; D002417:Cattle / Q000097:blood*; D005260:Female; D023421:Models, Animal; D012016:Reference Values
chemical_list: D000925:Anticoagulants; D000933:Antifibrinolytic Agents
keywords: Coagulation management; Coagulopathy; Direct oral anticoagulants; Jersey calves; Medical implants