title: Hollow Au-Ag Nanoparticles Labeled Immunochromatography Strip for Highly Sensitive Detection of Clenbuterol.
abstract: The probe materials play a significant role in improving the detection efficiency and sensitivity of lateral-flow immunochromatographic test strip (ICTS). Unlike conventional ICTS assay usually uses single-component, solid gold nanoparticles as labeled probes, in our present study, a bimetallic, hollow Au-Ag nanoparticles (NPs) labeled ICTS was successfully developed for the detection of clenbuterol (CLE). The hollow Au-Ag NPs with different Au/Ag mole ratio and tunable size were synthesized by varying the volume ratio of [HAuCl4]:[Ag NPs] via the galvanic replacement reaction. The surface of hollow Ag-Au NPs was functionalized with 11-mercaptoundecanoic acid (MUA) for further covalently bonded with anti-CLE monoclonal antibody. Overall size of the Au-Ag NPs, size of the holes within individual NPs and also Au/Ag mole ratio have been systematically optimized to amplify both the visual inspection signals and the quantitative data. The sensitivity of optimized hollow Au-Ag NPs probes has been achieved even as low as 2 ppb in a short time (within 15 min), which is superior over the detection performance of conventional test strip using Au NPs. The optimized hollow Au-Ag NPs labeled test strip can be used as an ideal candidate for the rapid screening of CLE in food samples.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.|Division of Polymer and Composite Materials, Ningbo Institute of Material Technology and Engineering Chinese Academy of Sciences, No. 1219 Zhongguan West Road, Zhenhai District, Ningbo 315201, China.
authors.forename: Jingyun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Division of Polymer and Composite Materials, Ningbo Institute of Material Technology and Engineering Chinese Academy of Sciences, No. 1219 Zhongguan West Road, Zhenhai District, Ningbo 315201, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Division of Polymer and Composite Materials, Ningbo Institute of Material Technology and Engineering Chinese Academy of Sciences, No. 1219 Zhongguan West Road, Zhenhai District, Ningbo 315201, China.
authors.forename: Youju
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Biotechnology, Kumaun University, Bhimtal-263136, Uttarakhand, India.
authors.forename: Anirban
authors.initials: A
authors.lastname: Dandapat
authors.affiliation: Division of Polymer and Composite Materials, Ningbo Institute of Material Technology and Engineering Chinese Academy of Sciences, No. 1219 Zhongguan West Road, Zhenhai District, Ningbo 315201, China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Dai
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.|Division of Polymer and Composite Materials, Ningbo Institute of Material Technology and Engineering Chinese Academy of Sciences, No. 1219 Zhongguan West Road, Zhenhai District, Ningbo 315201, China.
authors.forename: Ganggang
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Division of Polymer and Composite Materials, Ningbo Institute of Material Technology and Engineering Chinese Academy of Sciences, No. 1219 Zhongguan West Road, Zhenhai District, Ningbo 315201, China.
authors.forename: Xuefei
authors.initials: X
authors.lastname: Lu
authors.affiliation: Division of Polymer and Composite Materials, Ningbo Institute of Material Technology and Engineering Chinese Academy of Sciences, No. 1219 Zhongguan West Road, Zhenhai District, Ningbo 315201, China.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Lai
authors.affiliation: Division of Polymer and Composite Materials, Ningbo Institute of Material Technology and Engineering Chinese Academy of Sciences, No. 1219 Zhongguan West Road, Zhenhai District, Ningbo 315201, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D002976:Clenbuterol / Q000032:analysis*; D003116:Color; D006046:Gold / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D016062:Porosity; D012834:Silver / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013194:Staining and Labeling; D013499:Surface Properties
chemical_list: D012834:Silver; D006046:Gold; D002976:Clenbuterol