title: [CRISPR-Cas system as molecular scissors for gene therapy].
abstract: Since the discovery of the CRISPR-Cas system as the adaptive immune system of prokaryotes, the underlying mechanism has proven to be a precise molecular tool for the targeted editing of genetic information in various cell types. By using the CRISPR-Cas9 system DNA sequences can be cut out at any site in the genome and changed in a sequence-specific manner. In the long term this provides the opportunity to cure diseases caused by gene mutations.
authors.affiliation: Deutsches Rheuma-Forschungszentrum (DRFZ), Charitéplatz 1, 10117, Berlin, Deutschland.
authors.forename: G A
authors.initials: GA
authors.lastname: Heinz
authors.affiliation: Deutsches Rheuma-Forschungszentrum (DRFZ), Charitéplatz 1, 10117, Berlin, Deutschland. mashreghi@drfz.de.
authors.forename: M-F
authors.initials: MF
authors.lastname: Mashreghi
mesh_terms: D064113:CRISPR-Cas Systems / Q000235:genetics*; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D000072669:Gene Editing / Q000379:methods*; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D058990:Molecular Targeted Therapy / Q000379:methods*
keywords: CRISPR-Cas9; Gene mutations; Genome; Immune system; Prokaryotes