title: Targeting peptide functionalized liposomes towards aminopeptidase N for precise tumor diagnosis and therapy.
abstract: Aminopeptidase N (APN/CD13) is closely related to the growth of cancers and is suggested as a suitable target for anti-cancer therapy. Based on the "one-bead-one-compound" (OBOC) approach on a microarray device, we screened out a novel affinity peptide LN (YEVGHRC). It was determined that LN could specifically recognize and bind to APN. Moreover, LN-functionalized liposomes (LN-LS) could achieve efficient nano-encapsulated drug delivery under APN-overexpressing tumor conditions in vitro and in vivo. We expect that LN-LS could provide a new strategy for APN-positive tumor diagnosis and therapy.
authors.affiliation: Jinzhou Medical University, Jinzhou 121001, Liaoning, China. jiayunhong2012@163.com wangwz@nanoctr.cn huzy@nanoctr.cn and CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, P. R. China.
authors.forename: Xiangqian
authors.initials: X
authors.lastname: Jia
authors.affiliation: Jinzhou Medical University, Jinzhou 121001, Liaoning, China. jiayunhong2012@163.com wangwz@nanoctr.cn huzy@nanoctr.cn and CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, P. R. China.
authors.forename: Qiuju
authors.initials: Q
authors.lastname: Han
authors.affiliation: CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, P. R. China.
authors.forename: Zihua
authors.initials: Z
authors.lastname: Wang
authors.affiliation: CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, P. R. China and University of Chinese Academy of Science, No. 19A Yuquan Road, Beijing 100049, China and Sino-Danish Center for Education and Research, Beijing, 100190, China.
authors.forename: Yixia
authors.initials: Y
authors.lastname: Qian
authors.affiliation: Jinzhou Medical University, Jinzhou 121001, Liaoning, China. jiayunhong2012@163.com wangwz@nanoctr.cn huzy@nanoctr.cn.
authors.forename: Yunhong
authors.initials: Y
authors.lastname: Jia
authors.affiliation: CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, P. R. China.
authors.forename: Weizhi
authors.identifier: http://orcid.org/0000-0002-7310-4068
authors.initials: W
authors.lastname: Wang
authors.affiliation: CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, P. R. China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Hu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000627:therapeutic use; D018826:CD13 Antigens / Q000378:metabolism*; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000627:therapeutic use; D016503:Drug Delivery Systems / Q000379:methods*; D056945:Hep G2 Cells; D006801:Humans; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism*; D008819:Mice, Nude; D009369:Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D000970:Antineoplastic Agents; D008081:Liposomes; D010455:Peptides; D004317:Doxorubicin; D018826:CD13 Antigens