title: The Calibrated Phylogeny of the Drosophila fasciola Subgroup (D. repleta Group Wasserman) Indicates Neogene Diversification of Its Internal Branches.
abstract: The species of the Drosophila fasciola subgroup Wasserman represent the dominant section of the Drosophila repleta group Wasserman in the American rainforests and have a broad geographical distribution in the New World. However, despite of its wide range, the D. fasciola subgroup is one of the most overlooked D. repleta subgroups. Here, we report a molecular phylogenetic analysis focused on the D. fasciola subgroup using two mitochondrial [cytochrome oxidase subunit I (COI), cytochrome oxidase subunit II (COII)] and two nuclear [elongation factor-1alpha F1 (EF-alphaF1) and transformer (tra)] genes. Overall, we found that this subgroup is a monophyletic taxon, subdivided into two main internal branches: named Fas1 and Fas2 clades. The diversification of these clades is estimated to have begun in the middle Miocene, around 12 Ma [95% high posterior density (HPD) 9.0-15 Ma], and might be associated with the colonization of South America by Central America populations after the closure of Isthmus of Panama due to the temporal congruence between these events. The terminal branches had their origins estimated to be in the Pliocene or the Plio-Pleistocene transition. For the later estimates, both the geomorphological influences and the climatic oscillations of the Pleistocene may have played a role in shaping the diversification of the D. fasciola group.
authors.affiliation: Depto de Biologia, Centro de Ciências Humanas e Biológicas, Univ Federal de São Carlos, Rodovia João Leme dos Santos, Km 110, SP264, Sorocaba, 18052-780, Brasil. franco@ufscar.br.
authors.forename: F F
authors.initials: FF
authors.lastname: Franco
authors.affiliation: Secretaria do Meio Ambiente do Estado de São Paulo, São Paulo, Brasil.
authors.forename: E C C
authors.initials: ECC
authors.lastname: Silva
authors.affiliation: Pós-Graduação do Depto de Genética-Faculdade de Medicina de Ribeirão Preto, Univ de São Paulo, São Paulo, Brasil.
authors.forename: D Y
authors.initials: DY
authors.lastname: Barrios-Leal
authors.affiliation: Pós-Graduação do Depto de Genética-Faculdade de Medicina de Ribeirão Preto, Univ de São Paulo, São Paulo, Brasil.|Depto de Biologia-Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Univ de São Paulo, Ribeirão Preto, Brasil.
authors.forename: F M
authors.initials: FM
authors.lastname: Sene
authors.affiliation: Pós-Graduação do Depto de Genética-Faculdade de Medicina de Ribeirão Preto, Univ de São Paulo, São Paulo, Brasil.|Depto de Biologia-Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Univ de São Paulo, Ribeirão Preto, Brasil.
authors.forename: M H
authors.initials: MH
authors.lastname: Manfrin
mesh_terms: D000818:Animals; D005075:Biological Evolution; D002489:Central America; D004330:Drosophila / Q000145:classification*; D029721:Drosophila Proteins; D003576:Electron Transport Complex IV / Q000235:genetics; D017344:Genes, Insect; D009687:Nuclear Proteins / Q000235:genetics; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D010802:Phylogeny; D013020:South America
chemical_list: D029721:Drosophila Proteins; D009687:Nuclear Proteins; D020648:Peptide Elongation Factor 1; C097162:Tra protein, Drosophila; C117108:cytochrome C oxidase subunit II; D003576:Electron Transport Complex IV
keywords: Drosophila; Panamá Isthmus; elongation factor-1alpha F1; mtDNA; transformer