title: Low Incidence along with Low mRNA Levels ofEGFRvIIIin Prostate and Colorectal Cancers Compared to Glioblastoma.
abstract: Background:The presence as well as the potential role of EGFRvIIIin tumors other than glioblastoma still remains a controversial subject with many contradictory data published. Previous analyses, however, did not consider the level ofEGFRvIIImRNA expression in different tumor types.Methods:Appropriately designed protocol for Real-time quantitative reverse-transcription PCR (Real-time qRT-PCR) was applied to analyzeEGFRvIIIandEGFRWTmRNA expression in 155 tumor specimens. Additionally, Western Blot (WB) analysis was performed for selected samples. Stable cell lines showing EGFRvIIIexpression (CAS-1 and DK-MG) were analyzed by means of WB, immunocytochemistry (ICC) and fluorescencein situhybridization (FISH).Results:Our analyses revealedEGFRvIIIexpression in 27.59% of glioblastomas (8/29), 8.11% of colorectal cancers (3/37), 6.52% of prostate cancers (3/46) and none of breast cancers (0/43). Despite the average relative expression ofEGFRvIIIvarying greatly among tumors of different tissues (approximately 800-fold) or even within the same tissue group (up to 8000-fold for GB), even the marginal expression ofEGFRvIIImRNA can be detrimental to cancer progression, as determined by the analysis of stable cell lines endogenously expressing the oncogene.Conclusion:EGFRvIIIplays an unquestionable role in glioblastomas with high expression of this oncogene. Our data suggests that EGFRvIIIimportance should not be underestimated even in tumors with relatively low expression of this oncogene.
authors.affiliation: Department of Research and Development, Celther Polska, Ltd., Milionowa 23, 93-193 Lodz, Poland;; Department of Tumor Biology, Medical University of Lodz, Zeligowskiego 7/9, 90-752 Lodz, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Peciak
authors.affiliation: Department of Research and Development, Celther Polska, Ltd., Milionowa 23, 93-193 Lodz, Poland.
authors.forename: Wojciech J
authors.initials: WJ
authors.lastname: Stec
authors.affiliation: Department of Research and Development, Celther Polska, Ltd., Milionowa 23, 93-193 Lodz, Poland;; Department of Tumor Biology, Medical University of Lodz, Zeligowskiego 7/9, 90-752 Lodz, Poland.
authors.forename: Cezary
authors.initials: C
authors.lastname: Treda
authors.affiliation: Department of Research and Development, Celther Polska, Ltd., Milionowa 23, 93-193 Lodz, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Ksiazkiewicz
authors.affiliation: Department of Research and Development, Celther Polska, Ltd., Milionowa 23, 93-193 Lodz, Poland;; Department of Tumor Biology, Medical University of Lodz, Zeligowskiego 7/9, 90-752 Lodz, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Janik
authors.affiliation: Department of Research and Development, Celther Polska, Ltd., Milionowa 23, 93-193 Lodz, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Popeda
authors.affiliation: Department of Research and Development, Celther Polska, Ltd., Milionowa 23, 93-193 Lodz, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Smolarz
authors.affiliation: Department of Research and Development, Celther Polska, Ltd., Milionowa 23, 93-193 Lodz, Poland;; Department of Tumor Biology, Medical University of Lodz, Zeligowskiego 7/9, 90-752 Lodz, Poland.
authors.forename: Kamila
authors.initials: K
authors.lastname: Rosiak
authors.affiliation: Department of Research and Development, Celther Polska, Ltd., Milionowa 23, 93-193 Lodz, Poland.
authors.forename: Krystyna
authors.initials: K
authors.lastname: Hulas-Bigoszewska
authors.affiliation: Clinical Department of Neurosurgery, The Voivodal Specialistic Hospital in Olsztyn, Zolnierska 18, 10-561 Olsztyn, Poland.
authors.forename: Waldemar
authors.initials: W
authors.lastname: Och
authors.affiliation: Department of Research and Development, Celther Polska, Ltd., Milionowa 23, 93-193 Lodz, Poland;; Department of Tumor Biology, Medical University of Lodz, Zeligowskiego 7/9, 90-752 Lodz, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Rieske
authors.affiliation: Department of Research and Development, Celther Polska, Ltd., Milionowa 23, 93-193 Lodz, Poland;; Department of Tumor Biology, Medical University of Lodz, Zeligowskiego 7/9, 90-752 Lodz, Poland.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Stoczynska-Fidelus
keywords: EGFRvIII; Real-time quantitative reverse-transcription PCR.; breast cancer; colorectal cancer; glioblastoma; prostate cancer