title: Nanoengineered implant as a new platform for regenerative nanomedicine using 3D well-organized human cell spheroids.
abstract: In tissue engineering, it is still rare today to see clinically transferable strategies for tissue-engineered graft production that conclusively offer better tissue regeneration than the already existing technologies, decreased recovery times, and less risk of complications. Here a novel tissue-engineering concept is presented for the production of living bone implants combining 1) a nanofibrous and microporous implant as cell colonization matrix and 2) 3D bone cell spheroids. This combination, double 3D implants, shows clinical relevant thicknesses for the treatment of an early stage of bone lesions before the need of bone substitutes. The strategy presented here shows a complete closure of a defect in nude mice calvaria after only 31 days. As a novel strategy for bone regenerative nanomedicine, it holds great promises to enhance the therapeutic efficacy of living bone implants.
authors.affiliation: INSERM (French National Institute of Health and Medical Research), "Osteoarticular and Dental Regenerative Nanomedicine" Laboratory, UMR 1109, Faculté de Médecine, FMTS; University of Strasbourg, Faculté de Chirurgie Dentaire.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Keller
authors.affiliation: INSERM (French National Institute of Health and Medical Research), "Osteoarticular and Dental Regenerative Nanomedicine" Laboratory, UMR 1109, Faculté de Médecine, FMTS; University of Strasbourg, Faculté de Chirurgie Dentaire.
authors.forename: Ysia
authors.initials: Y
authors.lastname: Idoux-Gillet
authors.affiliation: INSERM (French National Institute of Health and Medical Research), "Osteoarticular and Dental Regenerative Nanomedicine" Laboratory, UMR 1109, Faculté de Médecine, FMTS; University of Strasbourg, Faculté de Chirurgie Dentaire.
authors.forename: Quentin
authors.initials: Q
authors.lastname: Wagner
authors.affiliation: INSERM (French National Institute of Health and Medical Research), "Osteoarticular and Dental Regenerative Nanomedicine" Laboratory, UMR 1109, Faculté de Médecine, FMTS; University of Strasbourg, Faculté de Chirurgie Dentaire.
authors.forename: Sandy
authors.initials: S
authors.lastname: Eap
authors.affiliation: CNRS (Centre National de la Recherche Scientifique), UMR 7178, IPHC (Hubert Curien Multidisciplinary Institute), Strasbourg, France.
authors.forename: David
authors.initials: D
authors.lastname: Brasse
authors.affiliation: INSERM (French National Institute of Health and Medical Research), "Osteoarticular and Dental Regenerative Nanomedicine" Laboratory, UMR 1109, Faculté de Médecine, FMTS; University of Strasbourg, Faculté de Chirurgie Dentaire.
authors.forename: Pascale
authors.initials: P
authors.lastname: Schwinté
authors.affiliation: Department of Chemical Engineering, INA (Aragon Nanoscience Institute), University of Zaragoza, Zaragoza, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Arruebo
authors.affiliation: INSERM (French National Institute of Health and Medical Research), "Osteoarticular and Dental Regenerative Nanomedicine" Laboratory, UMR 1109, Faculté de Médecine, FMTS; University of Strasbourg, Faculté de Chirurgie Dentaire.
authors.forename: Nadia
authors.initials: N
authors.lastname: Benkirane-Jessel
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects; D002113:Calcification, Physiologic / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D006801:Humans; D008819:Mice, Nude; D050997:Nanomedicine / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D011108:Polymers / Q000494:pharmacology; D016062:Porosity; D019736:Prostheses and Implants; D044968:Regenerative Medicine / Q000379:methods*; D018874:Spheroids, Cellular / Q000166:cytology*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D011108:Polymers
keywords: bioengineering; implants; matrix mineralization; microtissues; osteoblasts