title: Molecular and Quantum Mechanical Study for the Separation of Cefprozil in the Presence of Its Alkaline Degradation Product Using RP-HPLC with UV Detection.
abstract: A reversed-phase HPLC method (RP-HPLC) with UV detection was developed and validated for the quantitative determination of cefprozil, a second-generation cephalosporin. Due to β-lactam ring instability under alkaline conditions, this RP-HPLC method was applied for the determination of cefprozil in the presence of its possible degradation product. The interactions that govern the separation process with stationary phase were investigated at both molecular and quantum mechanical levels. Moreover, electrostatic potential maps were generated to determine the sites of interaction with mobile phase. The suggested method was validated in compliance with International Conference on Harmonization guidelines and successfully applied for the determination of cefprozil in its commercial pharmaceutical formulation.
authors.affiliation: Al-Azhar University, Faculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, 11751 Nasr City, Cairo, Egypt.
authors.forename: Khalid A M
authors.initials: KA
authors.lastname: Attia
authors.affiliation: Al-Azhar University, Faculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, 11751 Nasr City, Cairo, Egypt.
authors.forename: Mohammed W I
authors.initials: MW
authors.lastname: Nassar
authors.affiliation: Modern University for Technology and Information (MTI), Faculty of Pharmacy, Analytical Chemistry Department, 12582 Al Hadaba Al Wosta, Cairo, Egypt.
authors.forename: Mohamed B
authors.initials: MB
authors.lastname: El-Zeiny
authors.affiliation: Al-Azhar University, Faculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, 11751 Nasr City, Cairo, Egypt.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Serag
mesh_terms: D002511:Cephalosporins / Q000032:analysis*; D056148:Chromatography, Reverse-Phase; D057927:Hydrophobic and Hydrophilic Interactions; D008956:Models, Chemical; D010879:Piperazines / Q000032:analysis / Q000138:chemical synthesis; D011789:Quantum Theory; D012972:Sodium Hydroxide / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D000097670:Cefprozil
chemical_list: D002511:Cephalosporins; D010879:Piperazines; D012972:Sodium Hydroxide; D000097670:Cefprozil