title: Lnc-ATB contributes to gastric cancer growth through a MiR-141-3p/TGFβ2 feedback loop.
abstract: The long noncoding RNA (lncRNA) ATB is an important regulator in human tumors. Here, we aimed to investigate the potential molecular mechanisms of lnc-ATB in gastric cancer (GC) tumorigenesis. RT-qPCR analysis was used to detect lnc-ATB expression level in 20 pairs of gastric cancer tissues and adjacent normal gastric mucosa tissues (ANTs). Moreover, the biological role of lnc-ATB was determined in vitro. We found that lnc-ATB was significantly upregulated in GC tissues compared to lnc-ATB expression in ANTs. These high lnc-ATB expression levels predicted poor prognosis in GC patients. Low levels of lnc-ATB inhibited GC cell proliferation and cell cycle arrest in vitro. Lnc-ATB was found to directly bind miR-141-3p. Moreover, TGF-β actives lnc-ATB and TGF-β2 directly binds mir-141-3p. Finally, we demonstrated that lnc-ATB fulfilled its oncogenic roles in a ceRNA-mediated manner. Our study suggests that lnc-ATB promotes tumor progression by interacting with miR-141-3p and that Lnc-ATB may be a valuable prognostic predictor for GC. In conclusion, the positive feedback loop of lnc-ATB/miR-141-3p/TGF-β2 may be a potential therapeutic target for the treatment of GC.
authors.affiliation: State Key Laboratory of Bioreactor Engineering & Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, PR China.
authors.forename: Kecheng
authors.initials: K
authors.lastname: Lei
authors.affiliation: State Key Laboratory of Bioreactor Engineering & Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Bioreactor Engineering & Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, PR China.
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Bioreactor Engineering & Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, PR China.
authors.forename: Baixue
authors.initials: B
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Bioreactor Engineering & Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, PR China.
authors.forename: Yichun
authors.initials: Y
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Bioreactor Engineering & Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, PR China.
authors.forename: Yueqi
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou, Guangdong PR China. Electronic address: yywentao@aliyun.com.
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Tao
authors.affiliation: Department of General Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, PR China. Electronic address: weibindr@aliyun.com.
authors.forename: Weibin
authors.initials: W
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Bioreactor Engineering & Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, PR China. Electronic address: liujian@ecust.edu.cn.
authors.forename: Jianwen
authors.initials: J
authors.lastname: Liu
mesh_terms: D049109:Cell Proliferation; D025461:Feedback, Physiological; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D053781:Transforming Growth Factor beta2 / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
chemical_list: C555159:MIRN142 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding; C509041:TGFB2 protein, human; D053781:Transforming Growth Factor beta2; C000619854:long non-coding RNA ATB, human
keywords: Gastric cancer; TGF-β; lnc-ATB; miR-141-3p