title: Stromal Cell-Derived Growth Factor-1 Alpha-Elastin Like Peptide Fusion Protein Promotes Cell Migration and Revascularization of Experimental Wounds in Diabetic Mice.
abstract: Objective:In previous work, we demonstrated the development of a novel fusion protein containing stromal cell-derived growth factor-1 alpha juxtaposed to an elastin-like peptide (SDF1-ELP), which has similar bioactivity, but is more stable in elastase than SDF1. Herein, we compare the ability of a single topical application of SDF1-ELP to that of SDF1 in healing 1 × 1 cm excisional wounds in diabetic mice.Approach:Human Leukemia-60 cells were used to demonstrate the chemotactic potential of SDF1-ELP versus SDF1in vitro. Human umbilical vascular endothelial cells were used to demonstrate the angiogenic potential of SDF1-ELP versus SDF1in vitro. The bioactivity of SDF1-ELP versus SDF1 after incubation inex-vivodiabetic wound fluid was compared. Thein-vivoeffectiveness of SDF1-ELP versus SDF1 was compared in diabetic mice wound model by monitoring for the number of CD31+ cells in harvested wound tissues.Results:SDF1-ELP promotes the migration of cells and induces vascularization similar to SDF1in vitro. SDF1-ELP is more stable in wound fluids compared to SDF1.In vivo, SDF1-ELP induced a higher number of vascular endothelial cells (CD31+ cells) compared to SDF1 and other controls, suggesting increased vascularization.Innovation:While growth factors have been shown to improve wound healing, this strategy is largely ineffective in chronic wounds. In this work, we show that SDF1-ELP is a promising agent for the treatment of chronic skin wounds.Conclusion:The superiorin vivoperformance and stability of SDF1-ELP makes it a promising agent for the treatment of chronic skin wounds.
authors.affiliation: Department of Chemical and Biochemical Engineering, Rutgers University , Piscataway, New Jersey.
authors.forename: Agnes
authors.initials: A
authors.lastname: Yeboah
authors.affiliation: Department of Biomedical Engineering, Rutgers University , Piscataway, New Jersey.
authors.forename: Tim
authors.initials: T
authors.lastname: Maguire
authors.affiliation: Department of Biomedical Engineering, Rutgers University , Piscataway, New Jersey.
authors.forename: Rene
authors.initials: R
authors.lastname: Schloss
authors.affiliation: Department of Biomedical Engineering, Rutgers University , Piscataway, New Jersey.
authors.forename: Francois
authors.initials: F
authors.lastname: Berthiaume
authors.affiliation: Department of Biomedical Engineering, Rutgers University, Piscataway, New Jersey.; Center for Engineering in Medicine, Massachusetts General Hospital and Shriners Burns Hospital, Boston, Massachusetts.
authors.forename: Martin L
authors.initials: ML
authors.lastname: Yarmush
keywords: elastin like peptides (ELP); nanoparticles; skin; stromal cell-derived factor 1 (SDF1); wound healing