title: Differential gene expression by 1,25(OH)2D3in an endometriosis stromal cell line.
abstract: Endometriosis is a common female reproductive disease characterized by invasion of endometrial cells into other organs, frequently causing pelvic pain and infertility. Alterations of the vitamin D system have been linked to endometriosis incidence and severity. To shed light on the potential mechanism for these associations, we examined the effects of 1,25(OH)2D3on gene expression in endometriosis cells. Stromal cell lines derived from endometriosis tissue were treated with 1,25(OH)2D3, and RNA-seq was used to identify genes differentially expressed between treated and untreated cells. Gene ontology and pathway analyses were carried out using Partek Flow and Ingenuity software suites, respectively. We identified 1627 genes that were differentially expressed (886 down-regulated and 741 up-regulated) by 1,25(OH)2D3. Only one gene, CYP24A1, was strongly up-regulated (369-fold). Many genes were strongly down-regulated. 1,25(OH)2D3treatment down-regulated several genetic pathways related to neuroangiogenesis, cellular motility, and invasion, including pathways for axonal guidance, Rho GDP signaling, and matrix metalloprotease inhibition. These findings support a role for vitamin D in the pathophysiology of endometriosis, and provide new targets for investigation into possible causes and treatments.
authors.affiliation: University of Southern California, Department of Preventive Medicine, Los Angeles, CA 90089, USA; University of Southern California, Department of Obstetrics/Gynecology, Los Angeles, CA 90089, USA. Electronic address: ingles@usc.edu.
authors.forename: Sue Ann
authors.initials: SA
authors.lastname: Ingles
authors.affiliation: University of Southern California, Department of Preventive Medicine, Los Angeles, CA 90089, USA. Electronic address: lwu441@usc.edu.
authors.forename: Liang
authors.initials: L
authors.lastname: Wu
authors.affiliation: University of Southern California, Department of Preventive Medicine, Los Angeles, CA 90089, USA. Electronic address: liubenja@usc.edu.
authors.forename: Benjamin T
authors.initials: BT
authors.lastname: Liu
authors.affiliation: University of Southern California, Department of Health Science Libraries, Bioinformatics Service, Los Angeles, CA 90089, USA. Electronic address: yibuchen@usc.edu.
authors.forename: Yibu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: University of Southern California, Department of Obstetrics/Gynecology, Los Angeles, CA 90089, USA.
authors.forename: Chun-Yeh
authors.initials: CY
authors.lastname: Wang
authors.affiliation: University of Southern California, Department of Obstetrics/Gynecology, Los Angeles, CA 90089, USA.
authors.forename: Claire
authors.initials: C
authors.lastname: Templeman
authors.affiliation: University of Southern California, Department of Obstetrics/Gynecology, Los Angeles, CA 90089, USA. Electronic address: Doerthe.Brueggmann@med.usc.edu.
authors.forename: Doerthe
authors.initials: D
authors.lastname: Brueggmann
mesh_terms: D002117:Calcitriol / Q000378:metabolism*; D002460:Cell Line; D004715:Endometriosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D004717:Endometrium / Q000166:cytology / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology*; D015854:Up-Regulation / Q000187:drug effects; D065668:Vitamin D3 24-Hydroxylase / Q000235:genetics*; D014815:Vitamins / Q000378:metabolism
chemical_list: D014815:Vitamins; D065668:Vitamin D3 24-Hydroxylase; D002117:Calcitriol
keywords: CYP24A1; Differential gene expression; Endometriosis; Next generation sequencing; Vitamin D