title: Fast and sensitive quantification of protein SUMOylation using a dual luciferase biosensor.
abstract: Herein a convenient strategy was developed to quantify protein SUMOylation. The amount of target proteins and SUMO proteins conjugated to them could be quantified through luciferase-based bioluminescence detection and the relative bioluminescence was used to evaluate the SUMOylation degree of the target proteins.
authors.affiliation: Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. wanghz@wh.iov.cn.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xianliang
authors.initials: X
authors.lastname: Ke
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Zheng
authors.forename: Jin
authors.initials: J
authors.lastname: Meng
authors.forename: Hanzhong
authors.initials: H
authors.lastname: Wang
mesh_terms: D015374:Biosensing Techniques; D057809:HEK293 Cells; D006801:Humans; D008156:Luciferases / Q000737:chemistry*; D025841:Small Ubiquitin-Related Modifier Proteins / Q000737:chemistry*; D058207:Sumoylation
chemical_list: D025841:Small Ubiquitin-Related Modifier Proteins; D008156:Luciferases