title: Microbial succession in biofilms growing on artificial substratum in subtropical freshwater aquaculture ponds.
abstract: Biofilms can be used to improve the water quality in aquaculture ponds, and elucidating the process of microbial succession in biofilms would allow the characterization of metabolic processes and permit optimization. In the present study, microbial succession of a biofilm growing on artificial substrata in a subtropical freshwater pond was investigated by high-throughput sequencing. Providing artificial substrata effectively reduced the concentrations of total nitrogen and total phosphorus in the pond. Relatively stable microbiota were formed after approximately 1 week. The dominant phyla in the mature biofilm were Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria. The relative abundances of denitrifiers and phosphorus-removing bacteria, such as those in Comamonadaceae and Neisseriaceae, were significantly increased. The use of avermectin B1 changed the community structure of the microbiota; the microbiota were more similar to those at Week 0 than to those at Week 3. However, the microbial community structure recovered after approximately 1 week. Our results indicate that using artificial substrata can create a habitat for denitrifiers and phosphorus-removing bacteria, and thereby improve pond water quality. This study provided insight into how the use of artificial substrata could improve water quality and elucidated the environment-biofilm relationship in a subtropical freshwater pond.
authors.affiliation: Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou, China.
authors.forename: Zhifei
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou, China.|Tianjin Agricultural University, Tianjin, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Che
authors.affiliation: Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xie
authors.affiliation: Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou, China.
authors.forename: Guangjun
authors.initials: G
authors.lastname: Wang
authors.affiliation: Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou, China.
authors.forename: Ermeng
authors.initials: E
authors.lastname: Yu
authors.affiliation: Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou, China.
authors.forename: Deguang
authors.initials: D
authors.lastname: Yu
authors.affiliation: Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
mesh_terms: D039903:Actinobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D017756:Aquaculture; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D018441:Biofilms / Q000254:growth & development*; D005618:Fresh Water; D059014:High-Throughput Nucleotide Sequencing; D064307:Microbiota; D009584:Nitrogen / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism; D010802:Phylogeny; D061005:Ponds / Q000382:microbiology*; D020560:Proteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S; D013499:Surface Properties
chemical_list: D012336:RNA, Ribosomal, 16S; D010758:Phosphorus; D009584:Nitrogen
keywords: artificial substratum; bacterial community; biofilm; denitrifiers; microbiota succession; pond aquaculture