title: Effect of mesenchymal stem cells on small intestinal injury in a rat model of acute necrotizing pancreatitis.
abstract: Acute necrotizing pancreatitis (ANP) is often complicated by multiple organ failure. The small intestine is frequently damaged during ANP. Capillary leakage in multiple organs during ANP is one of the most important causes of multiple organ dysfunction. Damage to the capillary endothelial barrier and impaired water transportation could lead to capillary leakage in ANP.
  
  
  
  Sprague-Dawley (SD) rats were randomized into a control group, the ANP group, the culture media-treated group, or the bone marrow-derived mesenchymal stem cell (BMSC)-treated group (30 rats in each group). Ten rats in each group were sacrificed at 6, 12, and 24 h after induction of experimental models. Serum, ascites, pancreatic, and small intestinal samples were collected. The levels of serum and ascites albumin and amylases were measured, pancreatic histology was assessed, and the connection changes between vessel endothelial cells were evaluated using scanning electron microscopy (SEM). Capillary leakage in small intestinal tissue was observed visually by tracking fluorescein isothiocyanate (FITC)-albumin, and was measured by the Evans blue extravasation method. The location and expression of aquaporin 1 (AQP1) in the small intestine was analyzed using immunohistochemistry, real-time polymerase chain reaction (PCR), and Western blot.
  
  
  
  The outcomes showed that the level of serum and ascites amylase is elevated. Conversely, the level of serum albumin is decreased while ascites albumin is elevated. There is damage to pancreatic tissue, and the small intestinal capillary endothelial barrier was aggravated. Furthermore, the expression of AQP1 was reduced significantly after induced ANP. Following treatment with MSCs, the elevation of amylase and the decrease of serum albumin were inhibited, the damage to pancreatic tissue and the level of small intestinal capillary leakage was alleviated, and the downregulation of AQP1 was reversed.
  
  
  
  In conclusion, MSC therapy could alleviate small intestinal injury in rats with ANP, the mechanism of which might be related to reduction of damage to the small intestinal capillary endothelial barrier, and increased expression of AQP1 in the small intestine.
authors.affiliation: General Surgery Department, Fujian Medical University Union Hospital, Fuzhou, 350001, China.
authors.forename: Fengchun
authors.initials: F
authors.lastname: Lu
authors.affiliation: General Surgery Department, Fujian Medical University Union Hospital, Fuzhou, 350001, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: General Surgery Department, Fujian Medical University Union Hospital, Fuzhou, 350001, China.
authors.forename: Zhiyao
authors.initials: Z
authors.lastname: Chen
authors.affiliation: General Surgery Department, Fujian Medical University Union Hospital, Fuzhou, 350001, China. hhuang22@163.com.
authors.forename: Heguang
authors.initials: H
authors.lastname: Huang
mesh_terms: D000681:Amylases / Q000097:blood; D000818:Animals; D051398:Aquaporin 1 / Q000235:genetics / Q000378:metabolism; D001201:Ascites / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000473:pathology; D007421:Intestine, Small / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D010179:Pancreas / Q000473:pathology; D019283:Pancreatitis, Acute Necrotizing / Q000473:pathology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin / Q000032:analysis
chemical_list: D017077:Culture Media, Conditioned; D012333:RNA, Messenger; D012709:Serum Albumin; D051398:Aquaporin 1; D000681:Amylases
keywords: Acute necrotizing pancreatitis; Aquaporin 1; Capillary leakage; Intestinal injury; Mesenchymal stem cells