title: Effects of Different Ratio of n-6/n-3 Polyunsaturated Fatty Acids on the PI3K/Akt Pathway in Rats with Reflux Esophagitis.
abstract: BACKGROUND We designed this study to investigate the influence of different ratios of n-6/n-3 polyunsaturated fatty acid in the diet of reflux esophagitis (RE) rats' and the effect on the PI3K/Akt pathway. MATERIAL AND METHODS RE rats were randomly divided into a sham group and modeling groups of different concentrations of n-6/n-3 polyunsaturated fatty acid (PUFA): 12:1 group, 10:1 group, 5:1 group, and 1:1 group. RT-PCR and Western-blot were used to detect the expression of PI3K, Akt, p-Akt, NF-κBp50, and NF-κBp65 proteins in esophageal tissue. RESULTS In the n-6/n-3 PUFAs groups the expression of PI3K, Akt, p-Akt, nf-κbp50, and NF-κBp65 mRNA decreased with the decrease in n-6/n-3 ratios in the diet. The lowest expression of each indicator occurred in the 1:1 n-6/n-3 group compared with other n-6/n-3 groups, the difference was statistically significant (p<0.05). CONCLUSIONS The inhibition of n-3 PUFAs in the development of esophageal inflammation in rats with RE was attributed to the function of PI3K/Akt-NF-κB signaling pathway.
authors.affiliation: The School of Nursing, Fujian Medical University, Fuzhou, Fujian, China (mainland).
authors.forename: Jia-Yuan
authors.initials: JY
authors.lastname: Zhuang
authors.affiliation: The Second Affiliated Hospital, Fujian Medical University, Fuzhou, Fujian, China (mainland).
authors.forename: Zhi-Yao
authors.initials: ZY
authors.lastname: Chen
authors.affiliation: Fuzhou Second Hospital Affiliated to Xiamen University, Fuzhou, Fujian, China (mainland).
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: People's Hospital of Fujian Province, Fuzhou, Fujian, China (mainland).
authors.forename: Du-Peng
authors.initials: DP
authors.lastname: Tang
authors.affiliation: The School of Nursing, Fujian Medical University, Fuzhou, Fujian, China (mainland).
authors.forename: Xiao-Yin
authors.initials: XY
authors.lastname: Jiang
authors.affiliation: The First Affiliated Hospital, Fujian Medical University, Fuzhou, Fujian, China (mainland).
authors.forename: Ze-Hao
authors.initials: ZH
authors.lastname: Zhuang
mesh_terms: D000818:Animals; D004032:Diet; D004942:Esophagitis, Peptic / Q000178:diet therapy* / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage*; D043371:Fatty Acids, Omega-6 / Q000008:administration & dosage*; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000096:biosynthesis / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D016328:NF-kappa B; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt