title: In vivo differentiation of induced pluripotent stem cells into neural stem cells by chimera formation.
abstract: Like embryonic stem cells, induced pluripotent stem cells (iPSCs) can differentiate into all three germ layers in an in vitro system. Here, we developed a new technology for obtaining neural stem cells (NSCs) from iPSCs through chimera formation, in an in vivo environment. iPSCs contributed to the neural lineage in the chimera, which could be efficiently purified and directly cultured as NSCs in vitro. The iPSC-derived, in vivo-differentiated NSCs expressed NSC markers, and their gene-expression pattern more closely resembled that of fetal brain-derived NSCs than in vitro-differentiated NSCs. This system could be applied for differentiating pluripotent stem cells into specialized cell types whose differentiation protocols are not well established.
authors.affiliation: Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea.|Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Münster, Germany.
authors.forename: Hyun Woo
authors.initials: HW
authors.lastname: Choi
authors.affiliation: Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea.
authors.forename: Yean Ju
authors.initials: YJ
authors.lastname: Hong
authors.affiliation: Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea.
authors.forename: Jong Soo
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea.
authors.forename: Hyuk
authors.initials: H
authors.lastname: Song
authors.affiliation: Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea.
authors.forename: Ssang Gu
authors.initials: SG
authors.lastname: Cho
authors.affiliation: Department of Bioindustrial Technologies, College of Animal Bioscience and Technology, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea.
authors.forename: Hojae
authors.initials: H
authors.lastname: Bae
authors.affiliation: Department of Bioscience and Biotechnology, Sejong University, Gwangjin-gu, Seoul, Korea.
authors.forename: Changsung
authors.initials: C
authors.lastname: Kim
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, RDA, Iseo-myeon, Wanju-gun, Jeollabuk-do, Korea.
authors.forename: Sung June
authors.initials: SJ
authors.lastname: Byun
authors.affiliation: Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea.
authors.forename: Jeong Tae
authors.initials: JT
authors.lastname: Do
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D002678:Chimera / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D008822:Mice, Transgenic; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism