title: ANAQUIN: a software toolkit for the analysis of spike-in controls for next generation sequencing.
abstract: CONCLUSIONS
  Spike-in controls are synthetic nucleic-acid sequences that are added to a user's sample and constitute internal standards for subsequent steps in the next generation sequencing workflow.
  
  
  METHODS
  : The software is implemented in C ++/R and is freely available under BSD license. The source code is available from github.com/student-t/Anaquin , binaries and user manual from www.sequin.xyz/software and R package from bioconductor.org/packages/Anaquin.
  
  
  BACKGROUND
  anaquin@garvan.org.au or t.mercer@garvan.org.au.
  
  
  BACKGROUND
  Supplementary data are available at Bioinformatics online.
authors.affiliation: Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, NSW, Australia.
authors.forename: Ted
authors.initials: T
authors.lastname: Wong
authors.affiliation: Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, NSW, Australia.|Faculty of Science, School of Biotechnology and Biomolecular Sciences, UNSW, Sydney, NSW, Australia.
authors.forename: Ira W
authors.initials: IW
authors.lastname: Deveson
authors.affiliation: Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, NSW, Australia.|Faculty of Medicine, St Vincents Clinical School, UNSW, Sydney, NSW, Australia.
authors.forename: Simon A
authors.initials: SA
authors.lastname: Hardwick
authors.affiliation: Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, NSW, Australia.|Faculty of Medicine, St Vincents Clinical School, UNSW, Sydney, NSW, Australia.
authors.forename: Tim R
authors.initials: TR
authors.lastname: Mercer
mesh_terms: D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D017422:Sequence Analysis, DNA / Q000379:methods*; D017423:Sequence Analysis, RNA / Q000379:methods*; D012984:Software