title: A Lin28 homologue reprograms differentiated cells to stem cells in the moss Physcomitrella patens.
abstract: Both land plants and metazoa have the capacity to reprogram differentiated cells to stem cells. Here we show that the moss Physcomitrella patens Cold-Shock Domain Protein 1 (PpCSP1) regulates reprogramming of differentiated leaf cells to chloronema apical stem cells and shares conserved domains with the induced pluripotent stem cell factor Lin28 in mammals. PpCSP1 accumulates in the reprogramming cells and is maintained throughout the reprogramming process and in the resultant stem cells. Expression of PpCSP1 is negatively regulated by its 3'-untranslated region (3'-UTR). Removal of the 3'-UTR stabilizes PpCSP1 transcripts, results in accumulation of PpCSP1 protein and enhances reprogramming. A quadruple deletion mutant of PpCSP1 and three closely related PpCSP genes exhibits attenuated reprogramming indicating that the PpCSP genes function redundantly in cellular reprogramming. Taken together, these data demonstrate a positive role of PpCSP1 in reprogramming, which is similar to the function of mammalian Lin28.
authors.affiliation: National Institute for Basic Biology, Division of Evolutionary Biology, Okazaki 444-8585, Japan.|Department of Basic Biology, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Okazaki 444-8585, Japan.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.affiliation: National Institute for Basic Biology, Division of Evolutionary Biology, Okazaki 444-8585, Japan.|ERATO, Hasebe Reprogramming Evolution Project, Japan Science and Technology Agency, Okazaki 444-8585, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sako
authors.affiliation: National Institute for Basic Biology, Division of Evolutionary Biology, Okazaki 444-8585, Japan.|ERATO, Hasebe Reprogramming Evolution Project, Japan Science and Technology Agency, Okazaki 444-8585, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Imai
authors.affiliation: ERATO, Hasebe Reprogramming Evolution Project, Japan Science and Technology Agency, Okazaki 444-8585, Japan.|Advanced Science Research Center, Institute for Gene Research, Kanazawa University, Kanazawa 920-0934, Japan.
authors.forename: Tomoaki
authors.identifier: 0000-0003-1279-7806
authors.initials: T
authors.lastname: Nishiyama
authors.affiliation: National Institute for Basic Biology, Division of Evolutionary Biology, Okazaki 444-8585, Japan.|ERATO, Hasebe Reprogramming Evolution Project, Japan Science and Technology Agency, Okazaki 444-8585, Japan.|Division of Plant and Soil Sciences, West Virginia University, Morgantown, West Virginia 26506, USA.
authors.forename: Kari
authors.initials: K
authors.lastname: Thompson
authors.affiliation: National Institute for Basic Biology, Division of Evolutionary Biology, Okazaki 444-8585, Japan.|ERATO, Hasebe Reprogramming Evolution Project, Japan Science and Technology Agency, Okazaki 444-8585, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Kubo
authors.affiliation: National Institute for Basic Biology, Division of Evolutionary Biology, Okazaki 444-8585, Japan.|Department of Basic Biology, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Okazaki 444-8585, Japan.
authors.forename: Yuji
authors.identifier: 0000-0002-3996-0549
authors.initials: Y
authors.lastname: Hiwatashi
authors.affiliation: National Institute for Basic Biology, Division of Evolutionary Biology, Okazaki 444-8585, Japan.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kabeya
authors.affiliation: Division of Plant and Soil Sciences, West Virginia University, Morgantown, West Virginia 26506, USA.
authors.forename: Dale
authors.initials: D
authors.lastname: Karlson
authors.affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan.
authors.forename: Shu-Hsing
authors.identifier: 0000-0002-7179-3138
authors.initials: SH
authors.lastname: Wu
authors.affiliation: National Institute for Basic Biology, Division of Evolutionary Biology, Okazaki 444-8585, Japan.|Department of Basic Biology, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Okazaki 444-8585, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Ishikawa
authors.affiliation: National Institute for Basic Biology, Division of Evolutionary Biology, Okazaki 444-8585, Japan.|Department of Basic Biology, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Okazaki 444-8585, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Murata
authors.affiliation: Department of Biology and Howard Hughes Medical Institute, Duke University, Durham, North Carolina 27708, USA.
authors.forename: Philip N
authors.initials: PN
authors.lastname: Benfey
authors.affiliation: National Institute for Basic Biology, Division of Evolutionary Biology, Okazaki 444-8585, Japan.|ERATO, Hasebe Reprogramming Evolution Project, Japan Science and Technology Agency, Okazaki 444-8585, Japan.
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Sato
authors.affiliation: National Institute for Basic Biology, Division of Evolutionary Biology, Okazaki 444-8585, Japan.|Department of Basic Biology, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Okazaki 444-8585, Japan.
authors.forename: Yosuke
authors.identifier: 0000-0002-4845-4867
authors.initials: Y
authors.lastname: Tamada
authors.affiliation: National Institute for Basic Biology, Division of Evolutionary Biology, Okazaki 444-8585, Japan.|Department of Basic Biology, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Okazaki 444-8585, Japan.|ERATO, Hasebe Reprogramming Evolution Project, Japan Science and Technology Agency, Okazaki 444-8585, Japan.
authors.forename: Mitsuyasu
authors.initials: M
authors.lastname: Hasebe
mesh_terms: D020413:3' Untranslated Regions / Q000502:physiology; D019068:Bryopsida / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D065150:Cellular Reprogramming / Q000502:physiology*; D057773:Cold Shock Proteins and Peptides / Q000737:chemistry / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D018515:Plant Leaves / Q000166:cytology / Q000502:physiology; D010940:Plant Proteins / Q000737:chemistry / Q000502:physiology*; D030821:Plants, Genetically Modified; D000072417:Protein Domains / Q000502:physiology; D013234:Stem Cells / Q000502:physiology*
chemical_list: D020413:3' Untranslated Regions; D057773:Cold Shock Proteins and Peptides; D010940:Plant Proteins