title: Comparison of two rapid biochemical tests and four chromogenic selective media for detection of carbapenemase-producing Gram-negative bacteria.
abstract: We evaluated RAPIDEC® CARBA NP, Neo-Rapid CARB, chromID® CARBA SMART (CARB/OXA), Brilliance™ CRE/ESBL, ChromArt CRE and BBL™ CHROMagar™ CPE for the detection of carbapenemase-producing bacteria. The analytical sensitivity of RAPIDEC® CARBA NP was better than that of Neo-Rapid CARB. A combination of carbapenemase and ESBL screening plates could be advantageous.
authors.affiliation: Division of Clinical Microbiology, University Hospital Basel, Basel, Switzerland. Electronic address: vladimira.hinic@usb.ch.
authors.forename: Vladimira
authors.initials: V
authors.lastname: Hinić
authors.affiliation: Bioanalytica AG, Lucerne, Switzerland.
authors.forename: Ivo
authors.initials: I
authors.lastname: Amrein
authors.affiliation: Division of Clinical Microbiology, University Hospital Basel, Basel, Switzerland.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Stammler
authors.affiliation: Division of Clinical Microbiology, University Hospital Basel, Basel, Switzerland.
authors.forename: Judith
authors.initials: J
authors.lastname: Heckendorn
authors.affiliation: Division of Clinical Microbiology, University Hospital Basel, Basel, Switzerland; Applied Microbiology Research, Department of Biomedicine, University of Basel, Basel, Switzerland.
authors.forename: Dominik
authors.initials: D
authors.lastname: Meinel
authors.affiliation: Division of Clinical Microbiology, University Hospital Basel, Basel, Switzerland.
authors.forename: Reno
authors.initials: R
authors.lastname: Frei
authors.affiliation: Division of Clinical Microbiology, University Hospital Basel, Basel, Switzerland; Applied Microbiology Research, Department of Biomedicine, University of Basel, Basel, Switzerland.
authors.forename: Adrian
authors.initials: A
authors.lastname: Egli
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D001431:Bacteriological Techniques / Q000295:instrumentation* / Q000379:methods*; D002863:Chromogenic Compounds; D003124:Colorimetry / Q000379:methods; D003470:Culture Media / Q000737:chemistry; D006090:Gram-Negative Bacteria / Q000145:classification / Q000201:enzymology* / Q000254:growth & development; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013997:Time Factors; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins; D002863:Chromogenic Compounds; D003470:Culture Media; D011933:Reagent Kits, Diagnostic; D001618:beta-Lactamases; C063912:carbapenemase
keywords: Biochemical screening; Carbapenemase; Carbapenemase-producing Gram-negative bacteria; Chromogenic selective media; Resistance