title: In VitroAntifungal Susceptibility Testing of Candida Isolates with the EUCAST Methodology, a New Method for ECOFF Determination.
abstract: Thein vitrosusceptibilities of 1,099 molecularly identified clinicalCandidaisolates against 8 antifungal drugs were determined using the EUCAST microdilution method. A new simple, objective, and mathematically solid method for determining epidemiological cutoff values (ECOFFs) was developed by derivatizing the MIC distribution and determining the derivatized ECOFF (dECOFF) as the highest MIC with the maximum second derivative. The dECOFFs were similar (95% agreement within 1 dilution) to the EUCAST ECOFFs. Overall, low non-wild-type/resistance rates were found. The highest rates were found for azoles withC. parapsilosis(2.7 to 9.8%),C. albicans(7%), andC. glabrata(1.7 to 2.3%) and for echinocandins withC. krusei(3.3%),C. albicans(1%), andC. tropicalis(1.7%).
authors.affiliation: Clinical Microbiology Laboratory, Attikon University Hospital, Medical School, National and Kapodistrian University of Athens, Athens, Greece jmeletiadis@med.uoa.gr.|Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center, Rotterdam, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Meletiadis
authors.affiliation: Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands.
authors.forename: I
authors.initials: I
authors.lastname: Curfs-Breuker
authors.affiliation: Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands.|Center of Expertise in Mycology, Radboudumc, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands.
authors.forename: J F
authors.identifier: 0000-0003-3253-6080
authors.initials: JF
authors.lastname: Meis
authors.affiliation: Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center, Rotterdam, The Netherlands.
authors.forename: J W
authors.identifier: 0000-0001-6736-4149
authors.initials: JW
authors.lastname: Mouton
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D001393:Azoles / Q000494:pharmacology; D002175:Candida / Q000187:drug effects* / Q000378:metabolism*; D002176:Candida albicans / Q000187:drug effects / Q000378:metabolism; D041221:Candida glabrata / Q000187:drug effects / Q000378:metabolism; D025141:Drug Resistance, Fungal; D008826:Microbial Sensitivity Tests / Q000379:methods*; D008962:Models, Theoretical
chemical_list: D000935:Antifungal Agents; D001393:Azoles
keywords: Candida; EUCAST; antifungal susceptibility testing; epidemiological cutoff value