title: Gambogic acid inhibits the growth of ovarian cancer tumors by regulating p65 activity.
abstract: Ovarian cancer patients often have poor prognosis, therefore, it is important to search for more effective therapeutic strategies to treat them. Gambogic acid (GA) exhibits an anti-tumor effect through various mechanisms, and has multiple targets in tumor cells. The present study aimed to elucidate the efficacy of GA in the treatment of ovarian cancer bothin vivoandin vitroby analyzing its impact on cell survival and tumor growth through cell cycle and apoptosis analysis. GA inhibited the growth of ovarian cancer cells in a dose and time dependent manner, and arrested the cell cycle in ovarian cancer cells. Furthermore, GA increased caspase-3 and caspase-9 activity and inhibited RELA/NF-κB p65 (p65) DNA binding activity. Finally, GA suppressed tumor growthin vivo. Therefore, the current study suggests that GA inhibits the growth of ovarian cancer by regulating p65 activity, and may be developed as a novel therapeutic strategy to treat ovarian cancer.
authors.affiliation: Department of Pathology and Pathophysiology, Medical College, Southeast University, Nanjing, Jiangsu 210096, P.R. China.
authors.forename: Qiusha
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Genetic Laboratory, Wuxi Hospital for Maternal and Child Health Care, The Affiliated Hospital of Nanjing Medical University, Wuxi, Jiangsu 214002, P.R. China.
authors.forename: Mudan
authors.initials: M
authors.lastname: Lu
authors.affiliation: Department of Pathology and Pathophysiology, Medical College, Southeast University, Nanjing, Jiangsu 210096, P.R. China.
authors.forename: Huan
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Genetic Laboratory, Wuxi Hospital for Maternal and Child Health Care, The Affiliated Hospital of Nanjing Medical University, Wuxi, Jiangsu 214002, P.R. China.
authors.forename: Daozhen
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Pathology and Pathophysiology, Medical College, Southeast University, Nanjing, Jiangsu 210096, P.R. China.
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
keywords: anti-tumor activity; gambogic acid; ovarian cancer; p65 activity; tumor proliferation