title: Inhibition behaviours of some phenolic acids on rat kidney aldose reductase enzyme: an in vitro study.
abstract: Aldose reductase (AR) inhibitors have vital importance in the treatment and prevention of diabetic complications. In this study, rat kidney AR was purified 19.34-fold with a yield of 3.49% and a specific activity of 0.88 U/mg using DE-52 Cellulose anion exchange chromatography, gel filtration chromatography and 2'5' ADP Sepharose-4B affinity chromatography, respectively. After purification, the in vitro inhibition effects of some phenolic acids (tannic acid, chlorogenic acid, sinapic acid, protocatechuic acid, 4-hydroxybenzoic acid, p-coumaric acid, ferulic acid, vanillic acid, syringic acid, α-resorcylic acid, 3-hydroxybenzoic acid and gallic acid) were investigated on purified enzyme. We determined IC50, Kivalues and inhibition types of these phenolic acids. As a result, tannic and chlorogenic acid had a strong inhibition effect. On the other hand, gallic acid had a weak inhibition effect. In this study, all phenolic acids except for chlorogenic acid and p-coumaric acid showed non-competitive inhibition effects on rat kidney AR.
authors.affiliation: a Department of Chemistry, Faculty of Science and Arts , Ahi Evran University , Kirşehir , Turkey.
authors.forename: Zuhal
authors.initials: Z
authors.lastname: Alim
authors.affiliation: b Department of Medical Services and Techniques, Vocational School of Health Service , Iğdir University , Iğdir , Turkey.
authors.forename: Namik
authors.initials: N
authors.lastname: Kilinç
authors.affiliation: c Deparment of Health Care Service, Vocational School of Health Service , Bayburt University , Bayburt , Turkey.
authors.forename: Bülent
authors.initials: B
authors.lastname: Şengül
authors.affiliation: d Department of Chemistry, Faculty of Sciences , Atatürk University , Erzurum , Turkey.|e Department of Biochemistry, Faculty of Pharmacy , Anadolu University , Eskişehir , Turkey.
authors.forename: Şükrü
authors.initials: Ş
authors.lastname: Beydemir
mesh_terms: D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors*; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D062385:Hydroxybenzoates / Q000494:pharmacology*; D066298:In Vitro Techniques; D007668:Kidney / Q000201:enzymology*; D051381:Rats
chemical_list: D062385:Hydroxybenzoates; D000449:Aldehyde Reductase; C017616:phenolic acid
keywords: Aldose reductase; diabetic complications; inhibition; phenolic acids