title: Tissue-specific differentiation of colonic macrophages requires TGFβ receptor-mediated signaling.
abstract: Intestinal macrophages (mφ) form one of the largest populations of mφ in the body and are vital for the maintenance of gut homeostasis. They have several unique properties and are derived from local differentiation of classical Ly6Chimonocytes, but the factors driving this tissue-specific process are not understood. Here we have used global transcriptomic analysis to identify a unique homeostatic signature of mature colonic mφ that is acquired as they differentiate in the mucosa. By comparing the analogous monocyte differentiation process found in the dermis, we identify TGFβ as an indispensable part of monocyte differentiation in the intestine and show that it enables mφ to adapt precisely to the requirements of their environment. Importantly, TGFβR signaling on mφ has a crucial role in regulating the accumulation of monocytes in the mucosa, via mechanisms that are distinct from those used by IL10.
authors.affiliation: Centre for Immunobiology, Institute of Infection, Immunity and Inflammation, College of Medicine, Veterinary Medicine and Life Sciences, University of Glasgow, Scotland, UK.|Institute of Immunology, Hannover Medical School, Hannover, Germany.|Institute of Molecular Medicine, RWTH Aachen, Aachen, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Schridde
authors.affiliation: Centre for Immunobiology, Institute of Infection, Immunity and Inflammation, College of Medicine, Veterinary Medicine and Life Sciences, University of Glasgow, Scotland, UK.
authors.forename: C C
authors.initials: CC
authors.lastname: Bain
authors.affiliation: Centre for Immunobiology, Institute of Infection, Immunity and Inflammation, College of Medicine, Veterinary Medicine and Life Sciences, University of Glasgow, Scotland, UK.
authors.forename: J U
authors.initials: JU
authors.lastname: Mayer
authors.affiliation: Centre for Immunobiology, Institute of Infection, Immunity and Inflammation, College of Medicine, Veterinary Medicine and Life Sciences, University of Glasgow, Scotland, UK.
authors.forename: J
authors.initials: J
authors.lastname: Montgomery
authors.affiliation: Centre d'Immunologie de Marseille-Luminy, Aix Marseille Université, INSERM, CNRS, Marseille, France.
authors.forename: E
authors.initials: E
authors.lastname: Pollet
authors.affiliation: Genomics Facility IZKF, RWTH Aachen, Aachen, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Denecke
authors.affiliation: Centre for Immunobiology, Institute of Infection, Immunity and Inflammation, College of Medicine, Veterinary Medicine and Life Sciences, University of Glasgow, Scotland, UK.
authors.forename: S W F
authors.initials: SWF
authors.lastname: Milling
authors.forename: S J
authors.initials: SJ
authors.lastname: Jenkins
authors.affiliation: Centre d'Immunologie de Marseille-Luminy, Aix Marseille Université, INSERM, CNRS, Marseille, France.
authors.forename: M
authors.initials: M
authors.lastname: Dalod
authors.affiliation: Centre d'Immunologie de Marseille-Luminy, Aix Marseille Université, INSERM, CNRS, Marseille, France.
authors.forename: S
authors.initials: S
authors.lastname: Henri
authors.affiliation: Centre d'Immunologie de Marseille-Luminy, Aix Marseille Université, INSERM, CNRS, Marseille, France.
authors.forename: B
authors.initials: B
authors.lastname: Malissen
authors.affiliation: Institute of Immunology, Hannover Medical School, Hannover, Germany.|Institute of Molecular Medicine, RWTH Aachen, Aachen, Germany.
authors.forename: O
authors.initials: O
authors.lastname: Pabst
authors.affiliation: Centre for Immunobiology, Institute of Infection, Immunity and Inflammation, College of Medicine, Veterinary Medicine and Life Sciences, University of Glasgow, Scotland, UK.
authors.forename: A
authors.initials: A
authors.lastname: Mcl Mowat
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D060833:Cellular Microenvironment; D003106:Colon / Q000276:immunology*; D005260:Female; D020869:Gene Expression Profiling; D006706:Homeostasis; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009000:Monocytes / Q000276:immunology*; D009928:Organ Specificity; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D059467:Transcriptome; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: D000950:Antigens, Ly; C494192:Ly-6C antigen, mouse; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta