title: miR-214 down-regulates ARL2 and suppresses growth and invasion of cervical cancer cells.
abstract: Increasing evidence has shown that miRNAs are implicated in carcinogenesis and can function as oncogenes or tumor suppressor genes in human cancers. In this study, we confirmed that miR-214 is frequently down-regulated in cervical cancer compared with normal cervical tissues. Ectopic expression of miR-214 suppressed proliferation, migration and invasion of HeLa and C33A cervical cancer cells. Bioinformatics analysis revealed that ADP ribosylation factor like 2 (ARL2) was a potential target of miR-214 and was remarkably up-regulated in cervical cancer. Knockdown of ARL2 markedly inhibited cervical cancer cell proliferation, migration and invasion, similarly to over-expression of miR-214, indicating that ARL2 may function as an oncogene in cervical cancer. In conclusion, our study revealed that miR-214 acts as a tumor suppressor via inhibiting proliferation, migration and invasion of cervical cancer cells through targeting ARL2, and that both miR-214 and ARL2 may serve as prognostic or therapeutic targets for cervical cancer.
authors.affiliation: Precision medicine Center, Tianjin Medical University General Hospital, Tianjin, 300052, PR China.
authors.forename: Ruiqing
authors.initials: R
authors.lastname: Peng
authors.affiliation: Precision medicine Center, Tianjin Medical University General Hospital, Tianjin, 300052, PR China.
authors.forename: Jianlong
authors.initials: J
authors.lastname: Men
authors.affiliation: Precision medicine Center, Tianjin Medical University General Hospital, Tianjin, 300052, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Ma
authors.affiliation: Department of Clinical Laboratory Medicine, Tianjin Medical University General Hospital, Tianjin, 300052, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory Medicine, Tianjin Medical University General Hospital, Tianjin, 300052, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory Medicine, Tianjin Medical University General Hospital, Tianjin, 300052, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Precision medicine Center, Tianjin Medical University General Hospital, Tianjin, 300052, PR China. Electronic address: pengruiqing@yeah.net.
authors.forename: Jing
authors.initials: J
authors.lastname: Ren
mesh_terms: D017209:Apoptosis; D001665:Binding Sites; D049109:Cell Proliferation; D015536:Down-Regulation; D005260:Female; D019204:GTP-Binding Proteins / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D011485:Protein Binding; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology*
chemical_list: C547397:MIRN214 microRNA, human; D035683:MicroRNAs; C410130:ARL2 protein, human; D019204:GTP-Binding Proteins
keywords: ARL2; Cervical cancer; Growth; Invasion; Migration; miR-214