title: Increase in CD8+CD158a+ T Cells in Kidney Graft Blood is Associated with Better Renal Function.
abstract: BACKGROUND Studies of liver and heart transplant patients have shown a gradual reconstruction of the CD8 KIR2D+ T cell subpopulations, measured in peripheral blood (PB), associated with better graft acceptance. The kinetics of these populations in kidney transplants, however, is still poorly understood, especially given the lack of studies of blood samples from the kidney graft. MATERIAL AND METHODS Flow cytometry was used to measure CD8+CD158a/b/e T cells in 69 kidney transplant patients who had stable renal function during follow-up. Measurements were made at 3, 6, and 12 months post-transplantation in graft capillary blood extracted by fine needle aspiration puncture (FNAP) and in PB. RESULTS No progressive increase was found in the PB subpopulations. However, the CD8+CD158a+ subsets increased significantly at 12 months in the graft blood versus the PB samples (3.91±4.59 vs. 2.84±4.71; p=0.021). The ratio of the percentage of CD8+CD158a+ cells in graft blood compared to PB at 12 months was associated with better renal function in those patients with a ratio ≥3 (66.6±14.53 vs. 55.7±21.6; p=0.032). CONCLUSIONS An increased ratio of CD8+CD158a+ cells, measured by flow cytometry, between graft blood and PB was associated with improved renal function.
authors.affiliation: Department of Immunology, Carlos Haya Regional University Hospital, Institute of Biomedical Research in Malaga (IBIMA), University of Malaga, Malaga, Spain.
authors.forename: Abelardo
authors.initials: A
authors.lastname: Caballero
authors.affiliation: Department of Nephrology, Carlos Haya Regional University Hospital, Institute of Biomedical Research in Malaga (IBIMA), University of Malaga, Malaga, Spain.
authors.forename: Eulalia
authors.initials: E
authors.lastname: Palma
authors.affiliation: Department of Nephrology, Carlos Haya Regional University Hospital, Institute of Biomedical Research in Malaga (IBIMA), University of Malaga, Malaga, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Ruiz-Esteban
authors.affiliation: Department of Nephrology, Carlos Haya Regional University Hospital, Institute of Biomedical Research in Malaga (IBIMA), University of Malaga, Malaga, Spain.
authors.forename: Eugenia
authors.initials: E
authors.lastname: Sola
authors.affiliation: Department of Nephrology, Carlos Haya Regional University Hospital, Institute of Biomedical Research in Malaga (IBIMA), University of Malaga, Malaga, Spain.
authors.forename: Verónica
authors.initials: V
authors.lastname: López
authors.affiliation: Department of Nephrology, Carlos Haya Regional University Hospital, Institute of Biomedical Research in Malaga (IBIMA), University of Malaga, Malaga, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Fuentes
authors.affiliation: Department of Nephrology, Carlos Haya Regional University Hospital, Institute of Biomedical Research in Malaga (IBIMA), University of Malaga, Malaga, Spain.
authors.forename: Edisson
authors.initials: E
authors.lastname: Rudas
authors.affiliation: Department of Nephrology, Carlos Haya Regional University Hospital, Institute of Biomedical Research in Malaga (IBIMA), University of Malaga, Malaga, Spain.
authors.forename: Lara
authors.initials: L
authors.lastname: Perea
authors.affiliation: Department of Nephrology, Carlos Haya Regional University Hospital, Institute of Biomedical Research in Malaga (IBIMA), University of Malaga, Malaga, Spain.
authors.forename: Domingo
authors.initials: D
authors.lastname: Hernández
mesh_terms: D000328:Adult; D000368:Aged; D018414:CD8-Positive T-Lymphocytes; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007668:Kidney / Q000503:physiopathology*; D007677:Kidney Function Tests; D016030:Kidney Transplantation; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D011184:Postoperative Period; D013997:Time Factors