title: Octacosanol Attenuates Inflammation in Both RAW264.7 Macrophages and a Mouse Model of Colitis.
abstract: Octacosanol has multiple biological functions. In this study, the anti-inflammatory effect and molecular mechanism of octacosanol were evaluated by using dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice and lipopolysaccharide (LPS)-stimulated mouse macrophage RAW264.7 cells. The colitis mouse model was induced by 3.0% DSS in 8-week ICR mice and octacosanol orally administered with 100 mg/kg/day. The results showed that octacosanol significantly improved the health status of mice and reduced DSS-induced pathological damage in the colonic tissues. Octacosanol obviously inhibited the mRNA and protein expression levels of pro-inflammatory factors of colonic tissues. In vitro, octacosanol administration significantly reduced the expression of mRNA or protein of pro-inflammatory cytokines and the phosphorylation of c-Jun N-terminal kinase and p38, and it also partly prevented LPS-induced translocations of NF-κB and AP-1. Octacosanol has anti-inflammatory effect, and its molecular mechanism may be involved in downregulating the expression of inflammatory factors and blocking of MAPK/NF-κB/AP-1 signaling pathway.
authors.affiliation: Laboratory of Molecular Nutrition, National Engineering Laboratory for Rice and Byproducts, College of Food Science and Engineering, Central South University of Forestry and Technology , Changsha, Hunan 410004, China.
authors.forename: Tianyi
authors.initials: T
authors.lastname: Guo
authors.affiliation: Laboratory of Molecular Nutrition, National Engineering Laboratory for Rice and Byproducts, College of Food Science and Engineering, Central South University of Forestry and Technology , Changsha, Hunan 410004, China.
authors.forename: Qinlu
authors.initials: Q
authors.lastname: Lin
authors.affiliation: Department of Gastroenterology, Xiangya Hospital, Central South University , Changsha, Hunan 410008, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Li
authors.affiliation: Laboratory of Molecular Nutrition, National Engineering Laboratory for Rice and Byproducts, College of Food Science and Engineering, Central South University of Forestry and Technology , Changsha, Hunan 410004, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Nie
authors.affiliation: Laboratory of Molecular Nutrition, National Engineering Laboratory for Rice and Byproducts, College of Food Science and Engineering, Central South University of Forestry and Technology , Changsha, Hunan 410004, China.
authors.forename: Long
authors.initials: L
authors.lastname: Wang
authors.affiliation: Laboratory of Molecular Nutrition, National Engineering Laboratory for Rice and Byproducts, College of Food Science and Engineering, Central South University of Forestry and Technology , Changsha, Hunan 410004, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Shi
authors.affiliation: Laboratory of Molecular Nutrition, National Engineering Laboratory for Rice and Byproducts, College of Food Science and Engineering, Central South University of Forestry and Technology , Changsha, Hunan 410004, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.affiliation: Laboratory of Molecular Nutrition, National Engineering Laboratory for Rice and Byproducts, College of Food Science and Engineering, Central South University of Forestry and Technology , Changsha, Hunan 410004, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Hu
authors.affiliation: Laboratory of Molecular Nutrition, National Engineering Laboratory for Rice and Byproducts, College of Food Science and Engineering, Central South University of Forestry and Technology , Changsha, Hunan 410004, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Guo
authors.affiliation: Laboratory of Molecular Nutrition, National Engineering Laboratory for Rice and Byproducts, College of Food Science and Engineering, Central South University of Forestry and Technology , Changsha, Hunan 410004, China.
authors.forename: Feijun
authors.identifier: http://orcid.org/0000-0003-1951-4071
authors.initials: F
authors.lastname: Luo
mesh_terms: D000818:Animals; D003092:Colitis / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D005233:Fatty Alcohols / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000235:genetics / Q000276:immunology; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology; D012275:Oryza / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage*; D015398:Signal Transduction / Q000187:drug effects; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000276:immunology
chemical_list: D005233:Fatty Alcohols; D016328:NF-kappa B; D010936:Plant Extracts; D018808:Transcription Factor AP-1; D014409:Tumor Necrosis Factor-alpha; C044309:1-octacosanol; D048031:JNK Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases
keywords: AP-1; MAPK; NF-κB; colitis; inflammation factor; octacosanol