title: Disease progression and oxidative stress are associated with higher serum ferritin levels in patients with multiple sclerosis.
abstract: Hyperferritinemia and oxidative stress have been implicated in the pathogenesis of multiple sclerosis (MS). The aim of the present study was to evaluate the serum levels of ferritin and to verify their association with oxidative stress markers and MS progression. This study included 164 MS patients, which were divided in two groups according to their levels of ferritin (cut off 125.6μg/L). Oxidative stress was evaluated by tert-butyl hydroperoxide-initiated chemiluminescence (CL-LOOH), advanced oxidation protein products (AOPP), carbonyl protein, nitric oxide metabolites (NOx), sulfhydryl groups of protein and total radical-trapping antioxidant parameter (TRAP). MS patients with elevated levels of ferritin showed higher disease progression (p=0.030), AOPP (p=0.001), and lower plasma NOxlevels (p=0.031) and TRAP (p=0.006) than MS patients with lower ferritin levels. The multivariate binary logistic regression analysis showed that increased AOPP and progression of disease were significantly and positively associated with increase of ferritin. The combination of serum ferritin levels and oxidative stress markers were responsible for 13,9% in the disease progression. In conclusion, our results suggest that ferritin could aggravate oxidative stress in patients with MS and contribute to progression of disease.
authors.affiliation: Postgraduate Program, Biological Sciences Center, University of Londrina, Paraná, Brazil.
authors.forename: Katerine Panichi Zanin
authors.initials: KPZ
authors.lastname: Ferreira
authors.affiliation: Department of Pathology, Clinical Analysis and Toxicology, Health Sciences Center, University of Londrina, Paraná, Brazil.
authors.forename: Sayonara R
authors.initials: SR
authors.lastname: Oliveira
authors.affiliation: Postgraduate Program, Biological Sciences Center, University of Londrina, Paraná, Brazil.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Kallaur
authors.affiliation: Department of Internal Medicine, University of Londrina, Londrina, Paraná, Brazil.
authors.forename: Damacio R
authors.initials: DR
authors.lastname: Kaimen-Maciel
authors.affiliation: Department of Pathology, Clinical Analysis and Toxicology, Health Sciences Center, University of Londrina, Paraná, Brazil.
authors.forename: Marcell Alysson B
authors.initials: MAB
authors.lastname: Lozovoy
authors.affiliation: Department of Pathology, Clinical Analysis and Toxicology, Health Sciences Center, University of Londrina, Paraná, Brazil.
authors.forename: Elaine Regina Delicato
authors.initials: ERD
authors.lastname: de Almeida
authors.affiliation: Department of Pathology, Clinical Analysis and Toxicology, Health Sciences Center, University of Londrina, Paraná, Brazil.
authors.forename: Helena Kaminami
authors.initials: HK
authors.lastname: Morimoto
authors.affiliation: Department of Pathology, Clinical Analysis and Toxicology, Health Sciences Center, University of Londrina, Paraná, Brazil.
authors.forename: Leda
authors.initials: L
authors.lastname: Mezzaroba
authors.affiliation: Department of Internal Medicine, University of Londrina, Londrina, Paraná, Brazil.
authors.forename: Isaias
authors.initials: I
authors.lastname: Dichi
authors.affiliation: Department of Pathology, Clinical Analysis and Toxicology, Health Sciences Center, University of Londrina, Paraná, Brazil.
authors.forename: Edna Maria Vissoci
authors.initials: EMV
authors.lastname: Reiche
authors.affiliation: Department of Pathology, Clinical Analysis and Toxicology, Health Sciences Center, University of Londrina, Paraná, Brazil. Electronic address: deianame@yahoo.com.br.
authors.forename: Andréa Name Colado
authors.initials: ANC
authors.lastname: Simão
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D015992:Body Mass Index; D004185:Disability Evaluation; D018450:Disease Progression; D005260:Female; D005293:Ferritins / Q000097:blood*; D006801:Humans; D007155:Immunologic Factors / Q000627:therapeutic use; D007501:Iron / Q000097:blood; D016015:Logistic Models; D049449:Luminescence; D008297:Male; D009103:Multiple Sclerosis / Q000097:blood* / Q000188:drug therapy; D015999:Multivariate Analysis; D018384:Oxidative Stress / Q000502:physiology*; D012907:Smoking / Q000097:blood
chemical_list: D015415:Biomarkers; D007155:Immunologic Factors; D005293:Ferritins; D007501:Iron
keywords: Disease progression; Ferritin; Hyperferritinemia; Multiple sclerosis; Oxidative stress