title: Influence of random and oriented electrospun fibrous poly(lactic-co-glycolic acid) scaffolds on neural differentiation of mouse embryonic stem cells.
abstract: Engineering neural tissue by combining biodegradable materials, cells and growth factors is a promising strategy for the treatment of central and peripheral nervous system injuries. In this study, neural differentiation of mouse embryonic stem cells (mESCs) was investigated in combination with three dimensional (3D) electrospun nanofibers as a substitute for the extracellular matrix (ECM). Nano/microfibrous poly(lactic-co-glycolic acid) (PLGA) 3D scaffolds were fabricated through electrospinning and characterized. The scaffolds consisted of either a randomly oriented or an aligned structure of PLGA fibers. The mESCs were induced to differentiate into neuronal lineage and the effect of the polymer and fiber orientation on cell survival, morphology and differentiation efficiency was studied. The neural progenitors derived from the mESCs could survive and migrate onto the fibrous scaffolds. Aligned fibers provided more contact guidance with the neurites preferentially extending along the long axis of fiber. The mESCs differentiated into neural lineages expressing neural markers as seen by the immunocytochemistry. The nestin and beta3-tubulin expression was enhanced on the PLGA aligned fibers in comparison with the other groups, as seen by the quantitative analysis. Taken together, a combination of electrospun fiber scaffolds and mESC derived neural progenitor cells could provide valuable information about the effects of topology on neural differentiation and axonal regeneration. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1333-1345, 2017.
authors.affiliation: Hematology and Stem Cell Laboratory, Faculty of Pharmacy, Av. Ipiranga 2752, room 304G, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.|Stem Cell Laboratory, Fundamental Health Science Institute, Rua Sarmento Leite, 500, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Laura E
authors.initials: LE
authors.lastname: Sperling
authors.affiliation: Hematology and Stem Cell Laboratory, Faculty of Pharmacy, Av. Ipiranga 2752, room 304G, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.|Stem Cell Laboratory, Fundamental Health Science Institute, Rua Sarmento Leite, 500, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.|Post Graduate Program in Physiology, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Karina P
authors.initials: KP
authors.lastname: Reis
authors.affiliation: Hematology and Stem Cell Laboratory, Faculty of Pharmacy, Av. Ipiranga 2752, room 304G, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.|Stem Cell Laboratory, Fundamental Health Science Institute, Rua Sarmento Leite, 500, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Laura G
authors.initials: LG
authors.lastname: Pozzobon
authors.affiliation: Hematology and Stem Cell Laboratory, Faculty of Pharmacy, Av. Ipiranga 2752, room 304G, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Carolina S
authors.initials: CS
authors.lastname: Girardi
authors.affiliation: Hematology and Stem Cell Laboratory, Faculty of Pharmacy, Av. Ipiranga 2752, room 304G, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.|Stem Cell Laboratory, Fundamental Health Science Institute, Rua Sarmento Leite, 500, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.|Stem Cell Research Institute, Porto Alegre, RS, Brazil.
authors.forename: Patricia
authors.initials: P
authors.lastname: Pranke
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000096:biosynthesis; D002454:Cell Differentiation / Q000187:drug effects*; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D057139:Nanofibers / Q000737:chemistry*; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*; D011100:Polyglycolic Acid / Q000737:chemistry / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000943:Antigens, Differentiation; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: PLGA; electrospun scaffolds; embryonic stem cells; neural differentiation; substrate topography