title: Transcriptome profile of yeast reveals the essential role of PMA2 and uncharacterized gene YBR056W-A (MNC1) in adaptation to toxic manganese concentration.
abstract: Adaptation of S. cerevisiae to toxic concentrations of manganese provides a physiological model of heavy metal homeostasis. Transcriptome analysis of adapted yeast cells reveals upregulation of cell wall and plasma membrane proteins including membrane transporters. The gene expression in adapted cells differs from that of cells under short-term toxic metal stress. Among the most significantly upregulated genes are PMA2, encoding an ortholog of Pma1 H+-ATPase of the plasma membrane, and YBR056W-A, encoding a putative membrane protein Mnc1 that belongs to the CYSTM family and presumably chelates manganese at the cell surface. We demonstrate that these genes are essential for the adaptation to toxic manganese concentration and propose an extended scheme of manganese detoxification in yeast.
authors.affiliation: Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, 142290, Russia. alla@ibpm.pushchino.ru.
authors.forename: N
authors.initials: N
authors.lastname: Andreeva
authors.affiliation: Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, 142290, Russia. alla@ibpm.pushchino.ru.
authors.forename: E
authors.initials: E
authors.lastname: Kulakovskaya
authors.affiliation: Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, 142290, Russia. alla@ibpm.pushchino.ru.
authors.forename: A
authors.initials: A
authors.lastname: Zvonarev
authors.affiliation: Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow, 127051, Russia and A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119991, Russia and Laboratory of Extreme Biology, Institute of Fundamental Biology and Medicine, Kazan Federal University, Kazan, 420012, Russia and Faculty of Biology, Lomonosov Moscow State University, Moscow, 119991, Russia.
authors.forename: A
authors.initials: A
authors.lastname: Penin
authors.affiliation: Group of Protein Biosynthesis Regulation, Institute of Protein Research, Institutskaya 4, Pushchino, 142290, Russia.
authors.forename: I
authors.initials: I
authors.lastname: Eliseeva
authors.affiliation: Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkina 3, Moscow, GSP-1, 119991, Russia.
authors.forename: A
authors.initials: A
authors.lastname: Teterina
authors.affiliation: Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkina 3, Moscow, GSP-1, 119991, Russia and Moscow Institute of Physics and Technology (State University), Institutskiy per. 9, Dolgoprudny, Moscow Region 141700, Russia.
authors.forename: A
authors.initials: A
authors.lastname: Lando
authors.affiliation: Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkina 3, Moscow, GSP-1, 119991, Russia and Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilova 32, Moscow, GSP-1, 119991, Russia. ivan.kulakovskiy@gmail.com.
authors.forename: I V
authors.initials: IV
authors.lastname: Kulakovskiy
authors.affiliation: Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, 142290, Russia. alla@ibpm.pushchino.ru.
authors.forename: T
authors.identifier: 0000-0001-8556-809X
authors.initials: T
authors.lastname: Kulakovskaya
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects*; D002462:Cell Membrane / Q000378:metabolism; D008345:Manganese / Q000633:toxicity*; D006180:Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome / Q000187:drug effects*
chemical_list: C434364:PMA2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D008345:Manganese; D006180:Proton-Translocating ATPases