title: A one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine.
abstract: Klebsiella pneumoniae, Klebsiella variicola and Klebsiella quasipneumoniae are difficult to differentiate phenotypically, leading to misinterpretation of their infection prevalence. We propose a multiplex PCR for blaSHV, blaLENand blaOKPand their flanking gene (deoR). Since this scheme focuses only on chromosomal genes, it will be feasible for Klebsiella identification in the clinical routine.
authors.affiliation: Laboratório de Genética Molecular de Microrganismos, Instituto Oswaldo Cruz/FIOCRUZ, Avenida Brasil 4365, Manguinhos, Rio de Janeiro, Brazil. Electronic address: ericafon@ioc.fiocruz.br.
authors.forename: Erica Lourenço
authors.initials: EL
authors.lastname: Fonseca
authors.affiliation: Laboratório de Genética Molecular de Microrganismos, Instituto Oswaldo Cruz/FIOCRUZ, Avenida Brasil 4365, Manguinhos, Rio de Janeiro, Brazil. Electronic address: nilceia.ramos@ioc.fiocruz.br.
authors.forename: Nilceia da Veiga
authors.initials: ND
authors.lastname: Ramos
authors.affiliation: Laboratório de Genética Molecular de Microrganismos, Instituto Oswaldo Cruz/FIOCRUZ, Avenida Brasil 4365, Manguinhos, Rio de Janeiro, Brazil. Electronic address: bruno@ioc.fiocruz.br.
authors.forename: Bruno G Nascimento
authors.initials: BG
authors.lastname: Andrade
authors.affiliation: Laboratório de Microbiologia Ambiental, Seção de Meio Ambiente, Instituto Evandro Chagas, Belém, Pará, Brazil. Electronic address: lenasa@iec.pa.gov.br.
authors.forename: Lena L C S
authors.initials: LL
authors.lastname: Morais
authors.affiliation: Laboratório de Genética Molecular de Microrganismos, Instituto Oswaldo Cruz/FIOCRUZ, Avenida Brasil 4365, Manguinhos, Rio de Janeiro, Brazil. Electronic address: mfabanto@ioc.fiocruz.br.
authors.forename: Michel F Abanto
authors.initials: MF
authors.lastname: Marin
authors.affiliation: Laboratório de Genética Molecular de Microrganismos, Instituto Oswaldo Cruz/FIOCRUZ, Avenida Brasil 4365, Manguinhos, Rio de Janeiro, Brazil. Electronic address: anapaulo@ioc.fiocruz.br.
authors.forename: Ana Carolina P
authors.initials: AC
authors.lastname: Vicente
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D007709:Klebsiella / Q000235:genetics*; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000235:genetics*; D008826:Microbial Sensitivity Tests / Q000379:methods; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D001618:beta-Lactamases
keywords: Class A β-lactamase; Klebsiella species; Molecular identification; bla(LEN); bla(OKP); bla(SHV)