title: Full-term potential of goat in vitro produced embryos after different cryopreservation methods.
abstract: Cryopreservation of preimplantation embryos represents a major challenge due to their shape and relatively large cells. Embryo source and cryopreservation method are key factors to cryotolerance efficiency and few reports have investigated more promising protocols for goat embryos. The study was aimed to compare different cryopreservation methods for goat in vitro produced (IVP) embryos. Goat blastocysts were subjected to conventional freezing (CF), Dimethyl sulfoxide vitrification (DMSO-V) and Dimethylformamide vitrification (DMF-V). Cryopreserved blastocysts were assessed for re-expansion, cell viability and in vivo development rates. Blastocyst re-expansion after cryopreservation was similar between groups, but cell viability was lower for DMF-V (32%) than CF (68%) and DMSO-V (60%). Pregnancy and delivery rates were similar for CF (60% and 50%) and DMSO-V (50% and 45%) and higher then DMF-V (20% and 15%), respectively. Finally, kidding rates were also indistinguishable for CF (40%) and DMSO-V (35%), but higher then DMF-V (12.5%). In conclusion, conventional freezing and vitrification using DMSO have similar efficiencies for cryopreservation of goat IVP embryos and cryoprotectant for vitrification affects its outcome.
authors.affiliation: Universidade Federal Rural de Pernambuco (UFRPE), Laboratório de Biotécnicas Aplicadas a Reprodução Animal, Recife, PE, Brazil. Electronic address: carlos.ztec@gmail.com.
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Ferreira-Silva
authors.affiliation: Universidade Federal Rural de Pernambuco (UFRPE), Laboratório de Biotécnicas Aplicadas a Reprodução Animal, Recife, PE, Brazil.
authors.forename: Marcelo Tigre
authors.initials: MT
authors.lastname: Moura
authors.affiliation: Centro de Tecnologias Estratégicas do Nordeste (CETENE), Recife, PE, Brazil.
authors.forename: Túlio Diego
authors.initials: TD
authors.lastname: Silva
authors.affiliation: Universidade Federal Rural da Amazônia, Parauapebas, PA, Brazil.
authors.forename: Luis Rennan Sampaio
authors.initials: LRS
authors.lastname: Oliveira
authors.affiliation: Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Adauto
authors.initials: A
authors.lastname: Chiamenti
authors.affiliation: Universidade Estadual do Ceará, Fortaleza, CE, Brazil.
authors.forename: Vicente José
authors.initials: VJ
authors.lastname: Figueirêdo Freitas
authors.affiliation: Universidade Federal Rural de Pernambuco (UFRPE), Laboratório de Biotécnicas Aplicadas a Reprodução Animal, Recife, PE, Brazil.
authors.forename: Marcos Antonio Lemos
authors.initials: MAL
authors.lastname: Oliveira
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004126:Dimethylformamide / Q000494:pharmacology; D004624:Embryo Transfer; D004622:Embryo, Mammalian; D005307:Fertilization in Vitro / Q000379:methods*; D005615:Freezing; D006041:Goats; D058989:Vitrification / Q000187:drug effects
chemical_list: D003451:Cryoprotective Agents; D004126:Dimethylformamide; D004121:Dimethyl Sulfoxide
keywords: Capra hircus; Caprine; Cryobanking; Embryo transfer; IVF