title: Lactate Detection in Tumor Cell Cultures Using Organic Transistor Circuits.
abstract: A biosensing platform based on an organic transistor circuit for metabolite detection in highly complex biological media is introduced. The sensor circuit provides inherent background subtraction allowing for highly specific, sensitive lactate detection in tumor cell cultures. The proposed sensing platform paves the way toward rapid, label-free, and cost-effective clinically relevant in vitro diagnostic tools.
authors.affiliation: Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC, Gardanne, 13541, France.
authors.forename: Marcel
authors.initials: M
authors.lastname: Braendlein
authors.affiliation: Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC, Gardanne, 13541, France.
authors.forename: Anna-Maria
authors.initials: AM
authors.lastname: Pappa
authors.affiliation: Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC, Gardanne, 13541, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Ferro
authors.affiliation: INSERM U1068, Institut Paoli-Calmettes, CRCM, CNRS, Aix Marseille Université, Marseille, 13009, France.
authors.forename: Alexia
authors.initials: A
authors.lastname: Lopresti
authors.affiliation: INSERM U1068, Institut Paoli-Calmettes, CRCM, CNRS, Aix Marseille Université, Marseille, 13009, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Acquaviva
authors.affiliation: INSERM U1068, Institut Paoli-Calmettes, CRCM, CNRS, Aix Marseille Université, Marseille, 13009, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Mamessier
authors.affiliation: Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC, Gardanne, 13541, France.
authors.forename: George G
authors.initials: GG
authors.lastname: Malliaras
authors.affiliation: Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC, Gardanne, 13541, France.
authors.forename: Róisín M
authors.initials: RM
authors.lastname: Owens
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D002417:Cattle; D018929:Cell Culture Techniques; D048271:Chitosan / Q000737:chemistry; D017097:Electric Impedance; D055664:Electrochemical Techniques / Q000379:methods*; D004867:Equipment Design; D005296:Ferrous Compounds / Q000737:chemistry; D006801:Humans; D019344:Lactic Acid / Q000032:analysis*; D007963:Leukocytes, Mononuclear / Q000737:chemistry; D000075163:Metallocenes / Q000737:chemistry; D006899:Mixed Function Oxygenases / Q000737:chemistry; D009369:Neoplasms / Q000737:chemistry* / Q000175:diagnosis*; D011137:Polystyrenes; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013876:Thiophenes; D014173:Transistors, Electronic
chemical_list: D005296:Ferrous Compounds; D000075163:Metallocenes; D011137:Polystyrenes; D013876:Thiophenes; C533756:poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate); D012710:Serum Albumin, Bovine; D019344:Lactic Acid; D048271:Chitosan; D006899:Mixed Function Oxygenases; C021497:lactate 2-monooxygenase; C004998:ferrocene
keywords: Warburg effect; Wheatstone bridge circuit; organic electrochemical transistor; poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate); tumor cell cultures