title: Metabolic Response of Human Osteoarthritic Cartilage to Biochemically Characterized Collagen Hydrolysates.
abstract: The most frequent disease of the locomotor system is osteoarthritis (OA), which, as a chronic joint disease, might benefit more from nutrition than acute illnesses. Collagen hydrolysates (CHs) are peptidic mixtures that are often used as nutraceuticals for OA. Three CHs were characterized biochemically and pharmacologically. Our biophysical (MALDI-TOF-MS, NMR, AFM) and fluorescence assays revealed marked differences between CHs of fish (Peptan®F 5000, Peptan®F 2000) and porcine (Mobiforte®) origin with respect to the total number of peptides and common peptides between them. Using a novel dual radiolabeling procedure, no CH modulated collagen biosynthesis in human knee cartilage explants. Peptan®F 2000 enhanced the activities of the aggrecanase ADMATS4 and ADMATS5 in vitro without loss of proteoglycan from cartilage explants; the opposite effect was observed with Mobiforte®. Interleukin (IL)-6, matrix metalloproteinase (MMP)-1, -3 and -13 levels were elevated in explants that were treated with Mobiforte®and Peptan®F 5000, but not with Peptan®F 2000. In conclusion, the heterogeneous peptide composition and disparate pharmacological effects between CHs suggest that the effect of a CH preparation cannot be extrapolated to other formulations. Thus, the declaration of a CH as a safe and effective nutraceutical requires a thorough examination of its pleiotropic effects.
authors.affiliation: Laboratory for Experimental Orthopaedics, Department of Orthopaedics, Justus Liebig University Giessen, Paul-Meimberg-Str. 3, 35392 Giessen, Germany. saskia.schadow@meckit.de.
authors.forename: Saskia
authors.initials: S
authors.lastname: Schadow
authors.affiliation: Laboratory for Experimental Orthopaedics, Department of Orthopaedics, Justus Liebig University Giessen, Paul-Meimberg-Str. 3, 35392 Giessen, Germany. Viktor.S.Simons@med.uni-giessen.de.
authors.forename: Viktor S
authors.initials: VS
authors.lastname: Simons
authors.affiliation: Protein Analytics, Department of Biochemistry, Faculty of Medicine, Justus Liebig University Giessen, Friedrichstr. 24, 35392 Giessen, Germany. Guenter.Lochnit@biochemie.med.uni-giessen.de.
authors.forename: Guenter
authors.initials: G
authors.lastname: Lochnit
authors.affiliation: Laboratory for Experimental Orthopaedics, Department of Orthopaedics, Justus Liebig University Giessen, Paul-Meimberg-Str. 3, 35392 Giessen, Germany. jens.kordelle@ekm-gi.de.|Agaplesion Evangelical Hospital Mittelhessen, Paul-Zipp-Str. 171, 35398 Giessen, Germany. jens.kordelle@ekm-gi.de.
authors.forename: Jens
authors.initials: J
authors.lastname: Kordelle
authors.affiliation: Institute of Experimental Physics, Department of Biophysics, Slovak Academy of Sciences, Watsonova 47, 040 01 Košice, Slovakia. gazova@saske.sk.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Gazova
authors.affiliation: RI-B-NT Research Institute of Bioinformatics and Nanotechnology, Franziusallee 177, 24148 Kiel, Germany. hcsiebert@aol.com.
authors.forename: Hans-Christian
authors.initials: HC
authors.lastname: Siebert
authors.affiliation: Laboratory for Experimental Orthopaedics, Department of Orthopaedics, Justus Liebig University Giessen, Paul-Meimberg-Str. 3, 35392 Giessen, Germany. Juergen.Steinmeyer@ortho.med.uni-giessen.de.
authors.forename: Juergen
authors.initials: J
authors.lastname: Steinmeyer
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D019902:Chondrocytes / Q000187:drug effects / Q000378:metabolism; D003094:Collagen / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D010450:Endopeptidases / Q000378:metabolism; D005399:Fishes / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D020782:Matrix Metalloproteinases / Q000378:metabolism; D018625:Microscopy, Atomic Force; D010003:Osteoarthritis / Q000378:metabolism*; D011492:Protein Hydrolysates / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D011509:Proteoglycans / Q000378:metabolism; D019947:Receptors, Interleukin-6 / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013552:Swine; D046509:Tissue Culture Techniques; D019714:Tissue Inhibitor of Metalloproteinases / Q000378:metabolism
chemical_list: D015850:Interleukin-6; D011492:Protein Hydrolysates; D011509:Proteoglycans; D019947:Receptors, Interleukin-6; D019714:Tissue Inhibitor of Metalloproteinases; D003094:Collagen; D010450:Endopeptidases; D020782:Matrix Metalloproteinases; C083641:aggrecanase
keywords: ADAMTS; IL6, MALDI-TOF; MMP; Mobiforte®; Peptan® F; TIMP; cartilage; collagen hydrolysate; osteoarthritis