title: Identification and Characterization of a 25 kDa Protein That Is Indispensable for the Efficient Saccharification of Eisenia bicyclis in the Digestive Fluid of Aplysia kurodai.
abstract: The digestive fluid of the sea hare Aplysia kurodai can liberate approximately 2.5 mg of glucose from 10 mg of dried Eisenia bicyclis powder. Although laminaran, a major storage polysaccharide in E. bicyclis, is easily digested to glucose by the synergistic action of the 110 and 210 kDa A. kurodai β-glucosidases (BGLs), glucose is not liberated from E. bicyclis by direct incubation with these BGLs. To clarify this discrepancy, we searched for an Eisenia hydrolysis enhancing protein (EHEP) in the digestive fluid of A. kurodai. A novel 25 kDa protein that enhances E. bicyclis saccharification by β-glucosidases was purified to a homogeneous state from the digestive fluid of A. kurodai, and its cDNA was cloned from total cDNAs reverse-transcribed from hepatopancreas total RNA. The E. bicyclis extract strongly inhibited BGLs, suggesting some compound within this brown alga functioned as a feeding deterrent. However, when E. bicyclis was incubated with BGLs in the presence of EHEP, glucose production was markedly increased. As E. bicyclis is rich in phlorotannin, which are only found in brown algae, our study suggested that these compounds are the main BGL inhibitors in E. bicyclis extract. EHEP protects BGLs from phlorotannin inhibition by binding to phlorotannins and forming an insoluble complex with phloroglucinol and phlorotannins. These findings indicated that EHEP plays a key role in the saccharification of brown seaweeds containing phlorotannins in the digestive fluid of A. kurodai. This is the first report of EHEP as a phlorotannin-binding protein that protects BGLs from inhibition.
authors.affiliation: Department of Biomolecular function and Technology, Graduate School of Bioscience and Bioindustry, Tokushima University, 2-1 Minamijosanjima, Tokushima, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Tsuji
authors.affiliation: Department of Biomolecular function and Technology, Graduate School of Bioscience and Bioindustry, Tokushima University, 2-1 Minamijosanjima, Tokushima, Japan.
authors.forename: Shuji
authors.initials: S
authors.lastname: Kuwamura
authors.affiliation: Department of Bioresource Chemistry and Technology, Graduate School of Bioscience and Bioindustry, Tokushima University, 2-1 Minamijosanjima, Tokushima, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Shirai
authors.affiliation: Department of Biomolecular function and Technology, Graduate School of Bioscience and Bioindustry, Tokushima University, 2-1 Minamijosanjima, Tokushima, Japan.
authors.forename: Keizo
authors.initials: K
authors.lastname: Yuasa
mesh_terms: D000818:Animals; D001048:Aplysia / Q000235:genetics* / Q000378:metabolism; D044602:Cellulases / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004063:Digestion / Q000235:genetics*; D005936:Glucans / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006868:Hydrolysis; D000459:Phaeophyceae / Q000737:chemistry / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000235:genetics*; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D013634:Tannins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D005936:Glucans; D011134:Polysaccharides; D011506:Proteins; D013634:Tannins; C008247:laminaran; D044602:Cellulases; D005947:Glucose