title: Complete mitochondrial genomes of two flat-backed millipedes by next-generation sequencing (Diplopoda, Polydesmida).
abstract: A lack of mitochondrial genome data from myriapods is hampering progress across genetic, systematic, phylogenetic and evolutionary studies. Here, the complete mitochondrial genomes of two millipedes,Asiomorpha coarctataSaussure, 1860 (Diplopoda: Polydesmida: Paradoxosomatidae) andXystodesmussp. (Diplopoda: Polydesmida: Xystodesmidae) were assembled with high coverage using Illumina sequencing data. The mitochondrial genomes of the two newly sequenced species are circular molecules of 15,644 bp and 15,791 bp, within which the typical mitochondrial genome complement of 13 protein-coding genes, 22 tRNAs and two ribosomal RNA genes could be identified. The mitochondrial genome ofAsiomorpha coarctatais the first complete sequence in the family Paradoxosomatidae (Diplopoda: Polydesmida) and the gene order of the two flat-backed millipedes is novel among known myriapod mitochondrial genomes. Unique translocations have occurred, including inversion of one half of the two genomes with respect to other millipede genomes. Inversion of the entire side of a genome (trnF-nad5-trnH-nad4-nad4L,trnP,nad1-trnL2-trnL1-rrnL-trnV-rrnS,trnQ,trnCandtrnY) could constitute a common event in the order Polydesmida. Last, our phylogenetic analyses recovered the monophyletic Progoneata, subphylum Myriapoda and four internal classes.
authors.affiliation: College of Biology and Food Engineering, Chuzhou University, Chuzhou 239000, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Dong
authors.affiliation: College of Biology and Food Engineering, Chuzhou University, Chuzhou 239000, China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Zhu
authors.affiliation: College of Biology and Food Engineering, Chuzhou University, Chuzhou 239000, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Bai
authors.affiliation: College of Biology and Food Engineering, Chuzhou University, Chuzhou 239000, China.
authors.forename: Yongyue
authors.initials: Y
authors.lastname: Ou
authors.affiliation: College of Biology and Food Engineering, Chuzhou University, Chuzhou 239000, China.
authors.forename: Changbao
authors.initials: C
authors.lastname: Wang
keywords: Myriapoda; Polydesmida; gene order; mitochondrial genome; next-generation sequence