title: CHARACTERISTICS OF RAT LIVER EXPOSED TO NANOPARTICLES OF LEAD COMPOUNDS.
abstract: In recent times, the lead becomes great importance in environmental pollution, including its nanoparticles. In the literature, there is little data on the changes in the liver after the exposure with lead nanoparticles. The purpose of this study was the identification and determination of macroscopic, microscopic, and biochemical changes of the structural elements of the rat's liver exposed to the action of lead compounds. The study was carried out on 60 male Wistar rats. The first and second groups of animals were intraperitoneally injected with colloidal solution of nanoparticles of Lead Sulfide size of 10 nm and 30 nm, and the third group was intraperitoneally injected with a solution of nitrate lead. Macroscopic, histological, histochemical, biochemical methods and gas chromatography were used to identify the changes of fatty acids metabolism. The experiment has found that body weights of animals in all tested groups were decreased after 6 weeks of lead nanoparticles injection, while relative liver weight was increased. Levels of total lipids and cholesterol, total protein and albumin in the blood serum in study groups have decreased, and the level of triglycerides and glucose have increased. Moderate dystrophic changes were observed in the histological examinations of the liver, and this was confirmed by morphometric and densitometric parameters. Changes of fatty acid composition of lipids of the liver exposed to nanoparticles were the result of increasing arachidonic fatty acid content and reduction of the stearic fatty acid content. Thus, it has been proven by the experiment that the effect of lead nanoparticles depends on their size.
authors.affiliation: Bogomolets National medical university, Kyiv, Ukraine.
authors.forename: S
authors.initials: S
authors.lastname: Omelchuk
authors.affiliation: Bogomolets National medical university, Kyiv, Ukraine.
authors.forename: V
authors.initials: V
authors.lastname: Aleksijchuk
authors.affiliation: Bogomolets National medical university, Kyiv, Ukraine.
authors.forename: L
authors.initials: L
authors.lastname: Sokurenko
authors.affiliation: Bogomolets National medical university, Kyiv, Ukraine.
authors.forename: A
authors.initials: A
authors.lastname: Blagaia
authors.affiliation: Bogomolets National medical university, Kyiv, Ukraine.
authors.forename: S
authors.initials: S
authors.lastname: Prudchenko
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004785:Environmental Pollutants / Q000633:toxicity*; D005227:Fatty Acids / Q000378:metabolism; D007854:Lead / Q000633:toxicity*; D050356:Lipid Metabolism / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D053758:Nanoparticles / Q000633:toxicity*; D009566:Nitrates / Q000633:toxicity*; D009929:Organ Size / Q000187:drug effects; D017208:Rats, Wistar; D013440:Sulfides / Q000633:toxicity*
chemical_list: D004785:Environmental Pollutants; D005227:Fatty Acids; D009566:Nitrates; D013440:Sulfides; C018391:lead sulfide; D007854:Lead; C017461:lead nitrate