title: Preparation and Characterization of Hyaluronic Acid-Polycaprolactone Copolymer Micelles for the Drug Delivery of Radioactive Iodine-131 Labeled Lipiodol.
abstract: Micelles, with the structure of amphiphilic molecules including a hydrophilic head and a hydrophobic tail, are recently developed as nanocarriers for the delivery of drugs with poor solubility. In addition, micelles have shown many advantages, such as enhanced permeation and retention (EPR) effects, prolonged circulation times, and increased endocytosis through surface modification. In this study, we measured the critical micelle concentrations, diameters, stability, and cytotoxicity and the cell uptake of micelles against hepatic cells with two kinds of hydrophilic materials: PEG-PCL and HA-g-PCL. We used131I as a radioactive tracer to evaluate the stability, drug delivery, and cell uptake activity of the micelles. The results showed that HA-g-PCL micelles exhibited higher drug encapsulation efficiency and stability in aqueous solutions. In addition, the131I-lipiodol loaded HA-g-PCL micelles had better affinity and higher cytotoxicity compared to HepG2 cells.
authors.affiliation: Office of Research and Development, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Shih-Cheng
authors.initials: SC
authors.lastname: Chen
authors.affiliation: Center for Infectious Disease and Cancer Research, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Ming-Hui
authors.initials: MH
authors.lastname: Yang
authors.affiliation: Department of Biomedical Engineering, National Yang-Ming University, Taipei 112, Taiwan.
authors.forename: Tze-Wen
authors.initials: TW
authors.lastname: Chung
authors.affiliation: Invasive Cardiac Laboratory, Tan Tock Seng Hospital, Singapore 308433.
authors.forename: Ting-Syuan
authors.initials: TS
authors.lastname: Jhuang
authors.affiliation: Department of Chemistry, Chung Yuan Christian University, Taoyuan 320, Taiwan.
authors.forename: Jean-Dean
authors.initials: JD
authors.lastname: Yang
authors.affiliation: Department of Pathology, Changhua Christian Hospital, Changhua 500, Taiwan.
authors.forename: Ko-Chin
authors.initials: KC
authors.lastname: Chen
authors.affiliation: Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Wan-Jou
authors.initials: WJ
authors.lastname: Chen
authors.affiliation: Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Department of Nuclear Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.
authors.forename: Ying-Fong
authors.initials: YF
authors.lastname: Huang
authors.affiliation: Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Department of Nuclear Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.
authors.forename: Shiang-Bin
authors.initials: SB
authors.lastname: Jong
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.
authors.forename: Wan-Chi
authors.initials: WC
authors.lastname: Tsai
authors.affiliation: Department of Chemistry, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
authors.forename: Po-Chiao
authors.initials: PC
authors.lastname: Lin
authors.affiliation: Office of Research and Development, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Center for Infectious Disease and Cancer Research, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 804, Taiwan; Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.
authors.forename: Yu-Chang
authors.identifier: 0000-0002-1917-5893
authors.initials: YC
authors.lastname: Tyan
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003287:Contrast Media / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems; D004355:Drug Stability; D004998:Ethiodized Oil / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000633:toxicity; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D006820:Hyaluronic Acid / Q000031:analogs & derivatives; D057927:Hydrophobic and Hydrophilic Interactions; D007457:Iodine Radioisotopes / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000633:toxicity; D008823:Micelles; D010316:Particle Size; D011091:Polyesters; D019275:Radiopharmaceuticals / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000633:toxicity; D012995:Solubility
chemical_list: D003287:Contrast Media; D004337:Drug Carriers; D007457:Iodine Radioisotopes; D008823:Micelles; D011091:Polyesters; D019275:Radiopharmaceuticals; C531575:hyaluronic acid-polyethyleneglycol-polycaprolactone copolymer; C016240:polycaprolactone; D004998:Ethiodized Oil; D006820:Hyaluronic Acid