title: Identification of Arcanobacterium hippocoleae by MALDI-TOF MS analysis and by various genotypical properties.
abstract: In the present study an Arcanobacterium hippocoleae strain isolated from a uterus swab of an apparently healthy mare could be identified by phenotypic properties, by MALDI-TOF MS analysis and genotypically by investigating the molecular targets 16S rDNA, 16S-23S rDNA intergenic spacer region and the genes encoding the β subunit of bacterial RNA polymerase (rpoB), elongation factor tu (tuf) and glyceraldehyde 3-phosphate dehydrogenase (gap). The presented data are one of the few reports about the species A. hippocoleae and might help to elucidate the role this species plays in infections of horses.
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstraße 81, 35392 Gießen, Germany.
authors.forename: J-P
authors.initials: JP
authors.lastname: Wickhorst
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstraße 81, 35392 Gießen, Germany.
authors.forename: O
authors.initials: O
authors.lastname: Sammra
authors.affiliation: Department of Veterinary Public Health, College of Veterinary Medicine, University of Mosul, Mosul, Iraq.
authors.forename: A A
authors.initials: AA
authors.lastname: Hassan
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstraße 81, 35392 Gießen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Alssashen
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstraße 81, 35392 Gießen, Germany. Electronic address: christoph.laemmler@vetmed.uni-giessen.de.
authors.forename: C
authors.initials: C
authors.lastname: Lämmler
authors.affiliation: Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Gießen, Frankfurterstraße 85-91, 35392 Gießen, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Prenger-Berninghoff
authors.affiliation: RIPAC-LABOR GmbH, Am Mühlenberg 11, 14476 Potsdam-Golm, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Erhard
authors.affiliation: RIPAC-LABOR GmbH, Am Mühlenberg 11, 14476 Potsdam-Golm, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Metzner
authors.affiliation: Tierarztpraxis am Dorfteich, Am Dorfteich 8, 49661 Cloppenburg-Bethen, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Paschertz
authors.affiliation: Entwicklung Bioanalyse, Bruker Daltonik GmbH, Fahrenheitstraße 4, 28359 Bremen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Timke
authors.affiliation: Institut für Lebensmittelqualität und -sicherheit, Stiftung Tierärztliche Hochschule Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Abdulmawjood
mesh_terms: D000818:Animals; D055667:Arcanobacterium / Q000235:genetics / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000235:genetics; D005260:Female; D005838:Genotype; D006736:Horses / Q000382:microbiology*; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D017423:Sequence Analysis, RNA / Q000662:veterinary; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000662:veterinary; D014599:Uterus / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S
keywords: 16S rRNA gene; 16S-23S rRNA intergenic spacer region; Arcanobacterium hippocoleae; MALDI-TOF MS; gap; rpoB; tuf