title: Protein profiling of plasma proteins in dairy cows with subclinical hypocalcaemia.
abstract: Subclinical hypocalcaemia (SH) is an important metabolic disease in dairy cows that has a serious impact on production performance. The objective of this study was to investigate novel aspects of pathogenesis using proteomics technology to identify proteins that are differentially expressed in diseased and healthy animals. Dairy cows were divided into an SH group (T,n= 10) and a control group (C,n= 10) based on plasma calcium concentration. A total of 398 differentially expressed proteins were identified, of which 265 proteins were overlapped in the two parallel experiments. Of these, 24 differentially expressed proteins were statistically significant. Gene Ontology analysis yielded 74 annotations, including 7 cellular component, 55 biological process and 12 molecular function categories. Bioinformatics analysis indicated that calcium regulation, immune and inflammatory response, blood coagulation and complement pathway were all related to SH. Our iTRAQ/LC-MS/MS (isobaric tags for relative and absolute quantification/liquid chromatography-mass spectrometry/mass spectrometry) approach proved highly effective for plasma protein profiling of dairy cows with SH, and the results pave the way for further studies in this area.
authors.affiliation: Department of College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319 China.
authors.forename: Ziling
authors.initials: Z
authors.lastname: Fan
authors.affiliation: Department of College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319 China.
authors.forename: Shi
authors.initials: S
authors.lastname: Shu
authors.affiliation: Department of College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319 China.
authors.forename: Chuchu
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319 China.
authors.forename: Xinhuan
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Department of College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319 China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319 China.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Department of College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319 China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Xia
authors.affiliation: Department of College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319 China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319 China.
authors.forename: Hongyou
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319 China.
authors.forename: Chuang
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319 China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
keywords: Dairy cows; LC-MS/MS; Proteomics; Subclinical hypocalcaemia; iTRAQ