title: Nanoparticles Associate with Intrinsically Disordered RNA-Binding Proteins.
abstract: Nanoparticles are capable of penetrating cells, but little is known about the way they interact with intracellular proteome. Here we show that inorganic nanoparticles associate with low-complexity, intrinsically disordered proteins from HeLa cytosolic protein extracts in nondenaturing in vitro nanoparticle pull-down assays. Intrinsic protein disorder associates with structural mobility, suggesting that side-chain flexibility plays an important role in the driving of a protein to nanoparticle absorption. Disordered protein domains are often found in a diverse group of RNA-binding proteins. Consequently, the nanoparticle-associated proteomes were enriched in subunits of RNA-processing protein complexes. In turn, this indicates that within a cell, nanoparticles might interfere with protein synthesis triggering a range of cellular responses.
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Romashchenko
authors.affiliation: Department of Biochemistry, Erasmus Medical Center , Rotterdam 3015CE, The Netherlands.
authors.forename: Tsung-Wai
authors.initials: TW
authors.lastname: Kan
authors.affiliation: Institute of Systematics and Ecology of Animals, SB RAS , Novosibirsk 630091, Russia.
authors.forename: Dmitry V
authors.initials: DV
authors.lastname: Petrovski
authors.forename: Ludmila A
authors.initials: LA
authors.lastname: Gerlinskaya
authors.forename: Mikhail P
authors.initials: MP
authors.lastname: Moshkin
authors.forename: Yuri M
authors.identifier: 0000-0003-2964-8823
authors.initials: YM
authors.lastname: Moshkin
mesh_terms: D006367:HeLa Cells; D006801:Humans; D013058:Mass Spectrometry; D053758:Nanoparticles / Q000737:chemistry*; D040901:Proteomics; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D016601:RNA-Binding Proteins
keywords: RNA-binding proteins; intrinsically disordered proteins; nanoparticles; proteomics; stress granules