title: Single-Cell Metabolomics.
abstract: The dynamics of a cell is always changing. Cells move, divide, communicate, adapt, and are always reacting to their surroundings non-synchronously. Currently, single-cell metabolomics has become the leading field in understanding the phenotypical variations between them, but sample volumes, low analyte concentrations, and validating gentle sample techniques have proven great barriers toward achieving accurate and complete metabolomics profiling. Certainly, advanced technologies such as nanodevices and microfluidic arrays are making great progress, and analytical techniques, such as matrix-assisted laser desorption ionization (MALDI), are gaining popularity with high-throughput methodology. Nevertheless, live single-cell mass spectrometry (LCSMS) values the sample quality and precision, turning once theoretical speculation into present-day applications in a variety of fields, including those of medicine, pharmaceutical, and agricultural industries. While there is still room for much improvement, it is clear that the metabolomics field is progressing toward analysis and discoveries at the single-cell level.
authors.affiliation: Faculty of Pharmacy, Misr International University, Cairo, Egypt.
authors.forename: Samy
authors.initials: S
authors.lastname: Emara
authors.affiliation: Faculty of Pharmacy, Misr International University, Cairo, Egypt.|RIKEN, Quantitative Biology Center, Single Cell Mass Spectrometry Lab, 6-2-4 Furue-dai, Suita, Osaka, 565-0874, Japan.
authors.forename: Sara
authors.initials: S
authors.lastname: Amer
authors.affiliation: Faculty of Pharmacy, Misr International University, Cairo, Egypt.|RIKEN, Quantitative Biology Center, Single Cell Mass Spectrometry Lab, 6-2-4 Furue-dai, Suita, Osaka, 565-0874, Japan.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Ali
authors.affiliation: Faculty of Pharmacy, Misr International University, Cairo, Egypt.|RIKEN, Quantitative Biology Center, Single Cell Mass Spectrometry Lab, 6-2-4 Furue-dai, Suita, Osaka, 565-0874, Japan.
authors.forename: Yasmine
authors.initials: Y
authors.lastname: Abouleila
authors.affiliation: RIKEN, Quantitative Biology Center, Single Cell Mass Spectrometry Lab, 6-2-4 Furue-dai, Suita, Osaka, 565-0874, Japan.
authors.forename: April
authors.initials: A
authors.lastname: Oga
authors.affiliation: RIKEN, Quantitative Biology Center, Single Cell Mass Spectrometry Lab, 6-2-4 Furue-dai, Suita, Osaka, 565-0874, Japan. masujima@riken.jp.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Masujima
mesh_terms: D000383:Agriculture; D055808:Drug Discovery; D006801:Humans; D055432:Metabolomics / Q000379:methods*; D046210:Microfluidic Analytical Techniques; D059010:Single-Cell Analysis / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
keywords: Circulating tumor cell; Drug discovery; Live single-cell mass spectrometry; Mass spectrometry; Matrix-assisted laser desorption ionization; Metabolomics; Nanodevices; Single cell