title: Optimized Extraction of Resveratrol fromArachis repensHandro by Ultrasound and Microwave: A Correlation Study with the Antioxidant Properties and Phenol Contents.
abstract: The vegetal speciesArachis repens, commonly known as peanut grass, was studied and, for the first time, we detected the presence of the bioactive compoundtrans-resveratrol (t-RSV). We compared the efficiency of three different methodologies (conventional maceration [CM], ultrasound-assisted extractions [UAE], and microwave-assisted extractions [MAE]) concerning total phenolics (TP) and resveratrol (t-RSV) content, followed by antioxidant activity (AA) evaluation. By CM, at 1 h, the highest RSV content (1.024 ± 0.036 mg/L) and, correspondingly, the highest DPPH capture (23.90 ± 0.04%) were found. The TP contents, at 1 h, presented the highest value (27.26 ± 0.26 mg/g GAE). By the UAE, the maximum yields of TP (357.18 mg/g GAE) and RSV (2.14 mg/L), as well as, the highest AA (70.95%), were obtained by 5 min after a maceration pretreatment, on the solid-solvent ratio 1 : 40 w/v. For MAE, a central composite rotatable design (CCRD) was applied followed by the FFD design in order to evaluate the statistical effects of four independent variables on the extraction of RSV. The optimal conditions established for obtaining the highest recovery (2.516 mg/g) were 20 min; 90% MeOH aq.; 120 rpm; 60°C; and solid-solvent ratio: 1 : 35 w/v. Relevant correlations were established considering the TP and RSV contents, as well as the AA, corroborating obvious advantages of such techniques in terms of high extraction efficiency in shorter times.
authors.affiliation: Laboratório de Micropropagação e Transformação de Plantas, Cellular Biology Department, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Garcia
authors.affiliation: Laboratório de Micropropagação e Transformação de Plantas, Cellular Biology Department, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Renata
authors.initials: R
authors.lastname: Garcia
authors.affiliation: Laboratório de Micropropagação e Transformação de Plantas, Cellular Biology Department, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Georgia
authors.identifier: 0000-0001-5460-9899
authors.initials: G
authors.lastname: Pacheco
authors.affiliation: Departamento de Engenharia de Bioprocessos e Biotecnologia, Universidade Estadual Paulista (UNESP), Campus Botucatu, 18610 307 Botucatu, SP, Brazil.
authors.forename: Felipe
authors.initials: F
authors.lastname: Sutili
authors.affiliation: Biocatalysis and Organic Synthesis Group, Chemistry Institute, Federal University of Rio de Janeiro, 22941-909 Rio de Janeiro, RJ, Brazil.
authors.forename: Rodrigo De
authors.initials: R
authors.lastname: Souza
authors.affiliation: Laboratório de Micropropagação e Transformação de Plantas, Cellular Biology Department, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Elisabeth
authors.identifier: 0000-0001-6856-4425
authors.initials: E
authors.lastname: Mansur
authors.affiliation: Laboratório de Produtos Naturais e Ensaios Biológicos (LaProNEB), Pharmacy Faculty, Department of Natural Products and Food, Federal University of Rio de Janeiro, 21.941-902 Rio de Janeiro, RJ, Brazil.
authors.forename: Ivana
authors.identifier: 0000-0002-6917-9323
authors.initials: I
authors.lastname: Leal
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification*; D010367:Arachis / Q000737:chemistry*; D008872:Microwaves; D010636:Phenols / Q000737:chemistry*; D000077185:Resveratrol; D013267:Stilbenes / Q000302:isolation & purification*
chemical_list: D000975:Antioxidants; D010636:Phenols; D013267:Stilbenes; D000077185:Resveratrol