title: Infection Dynamics and Antimicrobial Resistance Profile of Salmonella Paratyphi B d-tartrate Positive (Java) in a Persistently Infected Broiler Barn.
abstract: The infection dynamics ofS.Java were examined in three consecutive rearing periods on a broiler farm in Northwestern Germany which had been persistently infected withS.Java for more than five years. The barn was investigated forSalmonellaoccurrence after cleaning and disinfection to verify the persistent contamination of the broiler house withS. Java before the start of the first rearing cycle. Confirmation ofSalmonellaabsence in day-old chicks (time-point 1) as well as early establishment of infection between days 5-7 (time-point 2) were confirmed by caecal swabs prepared for qPCR and classical microbiological methods. At three time-periods (between days 11-15 (time-point 3), days 25-28 (time-point 4), and days 38-40 (time-point 5)) caecal content was examined for colony forming units (CFU)Salmonella/g. In general, there was an increase inSalmonellaJava load at time-point 4 compared to time-points 3 and 5. Therefore, we observed a bell-shaped course of infection resulting in higher rates ofSalmonellaCFU/g prior to prethinning than at final slaughter. The antimicrobial susceptibility testing revealed resistance to tetracycline, fluorquinolones, trimethoprim, and cefoxitin.
authors.affiliation: Veterinary Practice and Laboratory Wilms-Ellert-Klosterhalfen, Lohe 13, D-49429 Visbek, Germany. franziska.kloska@tiho-hannover.de.|Institute of Food Quality and Food Safety, University of Veterinary Medicine, Bischofsholer Damm 15, D-30173 Hannover, Germany. franziska.kloska@tiho-hannover.de.
authors.forename: Franziska
authors.initials: F
authors.lastname: Kloska
authors.affiliation: Institute for Biometry, Epidemiology and Information Processing, University of Veterinary Medicine, Bünteweg 2, D-30559 Hannover, Germany. martin.beyerbach@tiho-hannover.de.
authors.forename: Martin
authors.initials: M
authors.lastname: Beyerbach
authors.affiliation: Institute of Food Quality and Food Safety, University of Veterinary Medicine, Bischofsholer Damm 15, D-30173 Hannover, Germany. guenter.klein@tiho-hannover.de.
authors.forename: Günter
authors.initials: G
authors.lastname: Klein
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002645:Chickens / Q000382:microbiology*; D024881:Drug Resistance, Bacterial; D005858:Germany; D006801:Humans; D007214:Indonesia; D012481:Salmonella Infections, Animal / Q000188:drug therapy*; D012484:Salmonella paratyphi B / Q000187:drug effects* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents
keywords: Infection dynamics; S. Java; antimicrobial resistance; broilers; veterinary public health