title: Impedimetric nanostructured genosensor for detection of schistosomiasis in cerebrospinal fluid and serum samples.
abstract: Schistosomiasis is a neglected disease closely related to the low levels of social development and a serious public health problem. In this work, we performed an electrochemical detection of Schistosoma mansoni DNA with a self-assembled monolayer of mercaptobenzoic acid (MBA) immobilizing nanostructures composed of gold nanoparticles (AuNPs) and magnetite nanoparticles (Fe3O4_NPs). Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were used to monitor the hybridization process. MBA-Fe3O4_NPs-AuNPs-DNAprobe system reveals an effective electrochemical response indicating the surface modification. The proposed biosystem was capable to recognize specific nucleotide sequence of S. mansoni present in cerebrospinal fluid (CFS) and serum samples at different genome DNA concentrations. The biorecognition resulted in an increase in the electron transfer resistance and a decrease of the current peaks at higher DNA concentrations during electrochemical measurements. The developed platform showed a DNA detection limit of 0.781 and 0.685pgμL-1for serum and CFS, respectively. Therefore, the obtained biosensor can be considered as a useful tool for specific detection of S. mansoni at low concentrations in various biological fluids.
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil.
authors.forename: Giselle S
authors.initials: GS
authors.lastname: Santos
authors.affiliation: Departamento de Bioquímica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil.
authors.forename: Cesar A S
authors.initials: CA
authors.lastname: Andrade
authors.affiliation: School of Medicine, University of Pernambuco, Recife, Pernambuco, Brazil.
authors.forename: Igor S
authors.initials: IS
authors.lastname: Bruscky
authors.affiliation: Aggeu Magalhaes Research Center/FIOCRUZ, Recife, Pernambuco, Brazil.
authors.forename: Leandro B
authors.initials: LB
authors.lastname: Wanderley
authors.affiliation: Laboratório de Doenças Transmissíveis, Centro de Pesquisas Aggeu Magalhães, 50670-420 Recife, PE, Brazil.
authors.forename: Fabio L
authors.initials: FL
authors.lastname: Melo
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil; Departamento de Bioquímica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil. Electronic address: m_danielly@yahoo.com.br.
authors.forename: Maria D L
authors.initials: MD
authors.lastname: Oliveira
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D018509:DNA, Helminth / Q000097:blood* / Q000134:cerebrospinal fluid*; D058266:Dielectric Spectroscopy / Q000379:methods; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D012550:Schistosoma mansoni / Q000235:genetics*; D012552:Schistosomiasis / Q000097:blood* / Q000134:cerebrospinal fluid*; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D018509:DNA, Helminth; D058185:Magnetite Nanoparticles; D013438:Sulfhydryl Compounds; D006046:Gold
keywords: Biosensors; Cyclic voltammetry; DNA; Impedance spectroscopy; Metal nanoparticles; Schistosomiasis