title: Effects of miRNA-200b on the development of diabetic retinopathy by targetingVEGFAgene.
abstract: The present study explored the effect ofmiR-200bon the development of diabetic retinopathy (DR) by targeting vascular endothelial growth factor A (VEGFA) gene. The study populations consisted of 255 DR patients (case group) and 253 healthy people (control group), while the expressions ofmiR-200band VEGFA mRNA were detected by quantitative real-time PCR (qRT-PCR). Bioinformatics software and dual-luciferase reporter assay were used to confirmVEGFAas a target gene ofmiR-200bAlso, a total of 70 Wistar male rats were selected and randomly assigned into blank, normal control (NC),miR-200bmimics,miR-200binhibitors,miR-200binhibitors + silencing vascular endothelial growth factor A (siVEGFA), and siVEGFA groups (n=10/group) respectively. Streptozotocin (STZ)-induced rat models of DR were successfully established. VEGFA, transforming growth factor-β1 (TGF-β1), hepatocyte growth factor (HGF), and pigment epithelium-derived factor (PEDF) were detected using qRT-PCR and Western blotting. In comparison with the control group, the case group showed lower expression ofmiR-200bbut higher expression ofVEGFAmRNA.VEGFAwas confirmed as a target gene ofmiR-200bRats in themiR-200bmimics and siVEGFA groups exhibited higher expression of PEDF mRNA and protein but lower expressions of VEGFA, TGF-β1, HGF protein, and mRNA than the NC group. There was no remarkable difference in expressions of PEDF, VEGFA, TGF-β1, HGF protein, and mRNA between themiR-200binhibitors + siVEGFA and NC groups. In conclusion, the present study demonstrated thatmiR-200bmight alleviate DR development by down-regulating its target geneVEGFA.
authors.affiliation: Department of Ophthalmology, Taizhou Hospital of Zhejiang Province, Taizhou 317000, P.R. China.
authors.forename: En-Hui
authors.initials: EH
authors.lastname: Li
authors.affiliation: Department of Ophthalmology, Taizhou Hospital of Zhejiang Province, Taizhou 317000, P.R. China.
authors.forename: Qin-Zhu
authors.initials: QZ
authors.lastname: Huang
authors.affiliation: Department of Ophthalmology, Taizhou Hospital of Zhejiang Province, Taizhou 317000, P.R. China.
authors.forename: Gao-Chun
authors.initials: GC
authors.lastname: Li
authors.affiliation: Department of Ophthalmology, Taizhou Hospital of Zhejiang Province, Taizhou 317000, P.R. China xiangzhengy29sda@163.com.
authors.forename: Zhen-Yang
authors.initials: ZY
authors.lastname: Xiang
authors.affiliation: Department of Ophthalmology, Taizhou Hospital of Zhejiang Province, Taizhou 317000, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000368:Aged; D000818:Animals; D015153:Blotting, Western; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000235:genetics / Q000378:metabolism; D003930:Diabetic Retinopathy / Q000209:etiology / Q000235:genetics*; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D017228:Hepatocyte Growth Factor / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015843:Serpins / Q000235:genetics / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics* / Q000378:metabolism; D000099174:Pigment Epithelium-Derived Factor
chemical_list: D005136:Eye Proteins; D017228:Hepatocyte Growth Factor; D035683:MicroRNAs; D009414:Nerve Growth Factors; D015843:Serpins; D053773:Transforming Growth Factor beta1; D042461:Vascular Endothelial Growth Factor A; D000099174:Pigment Epithelium-Derived Factor; C533896:MIRN200 microRNA, human; C555600:MIRN200 microRNA, rat
keywords: Diabetic retinopathy; Hepatocyte growth factor; Pigment epithelium derived factor; Vascular endothelial growth factor A; microRNA-200b