title: Expression analysis of matrix metalloproteinase-13 in human gastric cancer in the presence of Helicobacter Pylori infection.
abstract: BACKGROUND
  Matrix metalloproteinases (MMPs) can degrade essentially the extracellular matrix (ECM) components. MMPs are important regulators of tumor growth; hence the enzymes are considered as important targets for cancer therapy. MMP-13 is specially activated in gastric cancer and promotes the invasiveness of the primary tumors. Helicobacter Pylori (H.pylori) interacts with gastric epithelial cells and stimulates it to produce MMP-13in vitro.
  
  
  OBJECTIVE
  The relation between MMP-13 gene expression and clinicopathological characteristics of gastric cancer in the presence of H.pylori infection was investigated in fifty patients.
  
  
  METHODS
  The level of MMP-13 gene expression was measured by quantitative Real-time PCR method and was evaluated between two groups of normal and carcinomatous tissues.
  
  
  RESULTS
  The results showed 30% elevation of MMP-13 expression in tumor tissues. H.pylori infection did not have a significant effect on the expression of MMP-13. There was a correlation between gene expression and tumor type (P value = 0.032). In addition, there was a significant correlation between MMP-13 gene expression and tumor stage in intestinal group (P value = 0.023).
  
  
  CONCLUSIONS
  Based on the results, it might be concluded that in intestinal group, immune system plays an important role in reducing gene expression. Results also showed over expression (60%) in diffuse group. These findings suggest that using MMP-13 inhibitors in diffuse group might contribute to the control of tumor growth.
authors.affiliation: Department of Biophysics, Faculty of Science, Islamic Azad University-Mashhad Branch, Mashhad, Iran.
authors.forename: Shoaleh
authors.initials: S
authors.lastname: Sheibani
authors.affiliation: Immunology Research Center, BuAli Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Reihaneh Alsadat
authors.initials: RA
authors.lastname: Mahmoudian
authors.affiliation: Immunology Research Center, BuAli Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran.|Medical Genetics Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Abbaszadegan
authors.affiliation: Department of Biophysics, Faculty of Science, Islamic Azad University-Mashhad Branch, Mashhad, Iran.
authors.forename: Jamshidkhan
authors.initials: J
authors.lastname: Chamani
authors.affiliation: Pathology Department, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Bahram
authors.initials: B
authors.lastname: Memar
authors.affiliation: Department of Laboratory Sciences, School of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran.|Immunology Research Center, BuAli Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Mehran
authors.initials: M
authors.lastname: Gholamin
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D004847:Epithelial Cells / Q000382:microbiology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016481:Helicobacter Infections / Q000150:complications / Q000235:genetics* / Q000382:microbiology / Q000473:pathology; D016480:Helicobacter pylori / Q000472:pathogenicity; D006801:Humans; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000235:genetics*; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000150:complications / Q000235:genetics* / Q000382:microbiology / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13
keywords: Helicobacter Pylori; MMP-13; gastric cancer; real-time PCR