title: Metabolites of curculigoside in rats and their antiosteoporotic activities in osteoblastic MC3T3-E1 cells.
abstract: Curculigoside isolated from Curculiginis Rhizoma exhibits a wide spectrum of bioactivities. In this study, a high performance liquid chromatography/quadrupole time-of- flight tandem mass spectrometry (UHPLC/Q-TOF MS) method was employed to investigate the metabolism of curculigoside in rats. Plasma, bile, urine, feces and 17 tissues were collected from rats after a single PO dose of curculigoside at 100mg/kg and prepared through methanol precipitation. Parent compound and a total of 7 metabolites were detected and identified based on their retention time and fragment ions. Metabolic pathways of curculigoside in rats include hydrolysis, demethylation and glucuronidation. Exposure of major metabolite M2 in plasma and it's antiosteoporotic activity in osteoblastic MC3T3-E1 cells were studied to help understand that curculigoside assimilates less but works more.
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai, PR China.
authors.forename: Yong-Jing
authors.initials: YJ
authors.lastname: He
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Han
authors.affiliation: Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Lv
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai, PR China.
authors.forename: Yu-Qiong
authors.initials: YQ
authors.lastname: He
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai, PR China. Electronic address: zqy1965@163.com.
authors.forename: Qiao-Yan
authors.initials: QY
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai, PR China. Electronic address: hailiangxin@163.com.
authors.forename: Hai-Liang
authors.initials: HL
authors.lastname: Xin
mesh_terms: D000818:Animals; D001565:Benzoates / Q000493:pharmacokinetics*; D050071:Bone Density Conservation Agents / Q000493:pharmacokinetics*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D031408:Curculigo / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics; D005260:Female; D005960:Glucosides / Q000493:pharmacokinetics*; D008297:Male; D053858:Metabolic Networks and Pathways; D010006:Osteoblasts / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D027343:Rhizome / Q000737:chemistry; D053719:Tandem Mass Spectrometry
chemical_list: D001565:Benzoates; D050071:Bone Density Conservation Agents; D004365:Drugs, Chinese Herbal; D005960:Glucosides; C477170:curculigoside
keywords: Curculigoside; Metabolic profile; Metabolite, osteoporosis; UHPLC-Q-TOF MS