title: TGF-β1 promotes Staphylococcus aureus adhesion to and invasion into bovine mammary fibroblasts via the ERK pathway.
abstract: Fibroblasts are the structural base of mammary breast tissues. TGF-β1 can regulate the fibrotic process; however, it remains unclear whether TGF-β1 influences the susceptibility of fibroblasts to bacteria. Staphylococcus aureus (S. aureus) is a major bacterium in both chronic and subclinical mastitis in lactating cows that acts by invading host cells. To better understand the function of TGF-β1 in bovine mammary fibroblasts' (BMFBs) susceptibility to bacteria as well as the mechanisms involved, a primary BMFB model was established by treating cells with TGF-β1 followed by infection with S. aureus. The results revealed that the adhesion and invasion of S. aureus into BMFBs was significantly increased after cells were treated with 5 ng/ml TGF-β1 for 12 h. Moreover, TGF-β1 can increase Collagen I and α-SMA expression via activation of ERK signaling. However, the increased adhesion and invasion of S. aureus can be blocked by specific antibodies against either Collagen I or α-SMA, indicating that the increased adhesion and invasion are dependent on TGF-β1-induced upregulation of both Collagen I and α-SMA. Using PD98059, an ERK inhibitor, could also decrease the adhesion and invasion of S. aureus. These results indicate that TGF-β1 could promote S. aureus adhesion to and invasion into BMFBs by increasing Collagen I and α-SMA expression and may provide a novel target for controlling bovine mastitis.
authors.affiliation: College of Animal Science, Jilin University, Changchun, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhao
authors.affiliation: College of Animal Science, Jilin University, Changchun, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Xia
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun, China.
authors.forename: Yanyi
authors.initials: Y
authors.lastname: Che
authors.affiliation: College of Animal Science, Jilin University, Changchun, China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun, China.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Sun
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun, China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Ren
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun, China.
authors.forename: Wenyu
authors.initials: W
authors.lastname: Han
authors.affiliation: Department of Respiratory Medicine, The Second Hospital of Jilin University, Changchun, China.
authors.forename: Junling
authors.initials: J
authors.lastname: Yang
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun, China. Electronic address: leiliancheng@163.com.
authors.forename: Liancheng
authors.initials: L
authors.lastname: Lei
mesh_terms: D000199:Actins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D002417:Cattle; D002418:Cattle Diseases / Q000139:chemically induced; D018929:Cell Culture Techniques; D024042:Collagen Type I / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005355:Fibrosis / Q000382:microbiology / Q000662:veterinary; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D007774:Lactation; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D008321:Mammary Glands, Animal / Q000187:drug effects* / Q000378:metabolism; D008414:Mastitis, Bovine / Q000382:microbiology; D012333:RNA, Messenger / Q000096:biosynthesis; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000472:pathogenicity / Q000502:physiology*; D013997:Time Factors; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D000199:Actins; D024042:Collagen Type I; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1
keywords: Bovine mammary fibroblast; ERK; Staphylococcus aureus; TGF-β1