title: Evaluation of the protective effect of pVAX-EtMIC3-recombined plasmid against E. tenella in chicken.
abstract: Coccidiosis caused by protozoan parasites of the genus Eimeria has a severe economic impact on commercial production worldwide. Micronemes of Eimeria play important roles in invading intestinal cell processes. In this study, the DNA vaccine expressing Eimeria tenella microneme protein 3 (EtMIC3) was constructed to evaluate its immune protective effect against E. tenella infection in chickens. The results demonstrated that chickens immunized with pVAX-EtMIC3 produced strong immune responses in the body, as shown by significant lymphocyte proliferation, cytokine production, and antibody responses. The average body weight gains of chickens in all the vaccinated groups were higher than those of non-vaccinated and challenged groups. In general, oocyst shedding was reduced, and bloody feces and gut lesion scores decreased. In addition, the survival rate of the immunized chickens increased compared to that of the unvaccinated and challenged control chickens. In summary, this study indicated that pVAX-EtMIC3 could induce protective immune effects against coccidiosis and that EtMIC3 is a potential vaccine candidate against coccidiosis.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China.|Foshan Standard Bio-Tech Co. Ltd., Foshan, 528138, People's Republic of China.
authors.forename: Xin-Qiu
authors.initials: XQ
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China.
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Wu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China.|Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, Guangdong Province, 510642, People's Republic of China.
authors.forename: Ya-Biao
authors.initials: YB
authors.lastname: Weng
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China. rqlin@scau.edu.cn.|Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, Guangdong Province, 510642, People's Republic of China. rqlin@scau.edu.cn.
authors.forename: Rui-Qing
authors.initials: RQ
authors.lastname: Lin
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D002645:Chickens / Q000469:parasitology; D003048:Coccidiosis / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*; D004341:Drug Evaluation; D016786:Eimeria tenella / Q000235:genetics / Q000276:immunology*; D007114:Immunization; D033661:Oocysts / Q000276:immunology; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011201:Poultry Diseases / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control*; D015800:Protozoan Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D016052:Protozoan Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D019444:Vaccines, DNA
keywords: Emeria tenella; Immune protective effect; Micronemal proteins 3; pVAX-EtMIC3