title: Elevated-temperature-induced acceleration of PACT clearing process of mouse brain tissue.
abstract: Tissue optical clearing technique shows a great potential for neural imaging with high resolution, especially for connectomics in brain. The passive clarity technique (PACT) is a relative simple clearing method based on incubation, which has a great advantage on tissue transparency, fluorescence preservation and immunostaining compatibility for imaging tissue blocks. However, this method suffers from long processing time. Previous studies indicated that increasing temperature can speed up the clearing. In this work, we aim to systematacially and quantitatively study this influence based on PACT with graded increase of temperatures. We investigated the process of optical clearing of brain tissue block at different temperatures, and found that elevated temperature could accelerate the clearing process and also had influence on the fluorescence intensity. By balancing the advantages with drawbacks, we conclude that 42-47 °C is an alternative temperature range for PACT, which can not only produce faster clearing process, but also retain the original advantages of PACT by preserving endogenous fluorescence well, achieving fine morphology maintenance and immunostaining compatibility.
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, Hubei 430074, China.|Department of Biomedical Engineering, Key Laboratory of Biomedical Photonics, Ministry of Education, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, Hubei 430074, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Yu
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, Hubei 430074, China.|Department of Biomedical Engineering, Key Laboratory of Biomedical Photonics, Ministry of Education, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, Hubei 430074, China.
authors.forename: Yisong
authors.initials: Y
authors.lastname: Qi
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, Hubei 430074, China.|Department of Biomedical Engineering, Key Laboratory of Biomedical Photonics, Ministry of Education, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, Hubei 430074, China.
authors.forename: Jingtan
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, Hubei 430074, China.|Department of Biomedical Engineering, Key Laboratory of Biomedical Photonics, Ministry of Education, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, Hubei 430074, China.
authors.forename: Jianyi
authors.initials: J
authors.lastname: Xu
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, Hubei 430074, China.|Department of Biomedical Engineering, Key Laboratory of Biomedical Photonics, Ministry of Education, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, Hubei 430074, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Gong
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, Hubei 430074, China.|Department of Biomedical Engineering, Key Laboratory of Biomedical Photonics, Ministry of Education, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, Hubei 430074, China.
authors.forename: Qingming
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, Hubei 430074, China.|Department of Biomedical Engineering, Key Laboratory of Biomedical Photonics, Ministry of Education, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, Hubei 430074, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology*; D051379:Mice; D061848:Optical Imaging / Q000379:methods*; D010336:Pathology / Q000379:methods*; D013048:Specimen Handling / Q000379:methods*; D013696:Temperature