title: [Molecular detection of sexually transmitted agents in a symptomatic group of men and its relationship with sexual behavior].
abstract: BACKGROUND
  Sexually transmitted infections (STIs) affect sexual and reproductive health of millions of men. Pathogens such as human papillomavirus (HPV), herpes simplex virus type 1 and 2 (HSV-1 y HSV-2), Chlamydia trachomatis,Mycoplasmagenitalium,Mycoplasma hominis and Ureaplasma urealyticum are associated with STIs.
  
  
  OBJECTIVE
  To detect pathogens associated with STIs in symptomatic men and its relationship with sexual behavior.
  
  
  METHODS
  DNA was obtained from exfoliated cells of penis from 20 symptomatic men. Pathogens were detected using qPCR or PCR followed by reverse line blot. Sexual behavior was evaluated through a survey.
  
  
  RESULTS
  Two or more infectious agents were detected in 50% of samples. U. urealyticum was found in 25%, meanwhile C. trachomatis and M. hominis were detected in 15%. VHS-1, VHS-2 andM. genitalium were detected only in 5%. HPV was found in all samples. The most frequent HPV genotypes were VPH 16, 11, 70. There were no statistical link found between sexual behavior and the studied microorganisms Conclusion: Infectious agents associated with STIs were detected in symptomatic men. HPV was the most frequent pathogen and it was detected in multiple genotypes. It is necessary to increase the sample size to associate significantly the sexual behavior with the results.
authors.affiliation: Laboratorio de Patología Molecular, Departamento Anatomía Patológica, Facultad de Medicina, Universidad de La Frontera, Temuco, Chile.
authors.forename: Daniela
authors.initials: D
authors.lastname: León
authors.affiliation: Laboratorio de Patología Molecular, Departamento Anatomía Patológica, Facultad de Medicina, Universidad de La Frontera, Temuco, Chile.
authors.forename: Javier
authors.initials: J
authors.lastname: Retamal
authors.affiliation: Laboratorio de Patología Molecular, Departamento Anatomía Patológica, Facultad de Medicina, Universidad de La Frontera, Temuco, Chile.
authors.forename: Ramón
authors.initials: R
authors.lastname: Silva
authors.affiliation: Laboratorio de Patología Molecular, Departamento Anatomía Patológica, Facultad de Medicina, Universidad de La Frontera, Temuco, Chile.
authors.forename: Carmen
authors.initials: C
authors.lastname: Ili
authors.affiliation: Laboratorio de Patología Molecular, Departamento Anatomía Patológica, Facultad de Medicina, Universidad de La Frontera, Temuco, Chile.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Mieville
authors.affiliation: Laboratorio de Patología Molecular, Departamento Anatomía Patológica, Facultad de Medicina, Universidad de La Frontera, Temuco, Chile.
authors.forename: Pablo
authors.initials: P
authors.lastname: Guzmán
authors.affiliation: Servicio Dermatología, Hospital Hernán Henríquez Aravena, Temuco, Chile.
authors.forename: Gastón
authors.initials: G
authors.lastname: Briceño
authors.affiliation: Laboratorio de Patología Molecular, Departamento Anatomía Patológica, Facultad de Medicina, Universidad de La Frontera, Temuco, Chile.
authors.forename: Priscilla
authors.initials: P
authors.lastname: Brebi
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002692:Chlamydia trachomatis / Q000235:genetics* / Q000302:isolation & purification; D006561:Herpes Simplex / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009174:Mycoplasma / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012725:Sexual Behavior / Q000706:statistics & numerical data*; D012749:Sexually Transmitted Diseases / Q000382:microbiology* / Q000821:virology*; D014509:Ureaplasma / Q000235:genetics* / Q000302:isolation & purification; D055815:Young Adult