title: Sporosarcina terrae sp. nov., isolated from orchard soil.
abstract: A Gram-stain-positive, motile and rod-shaped bacterium, designated strain LZ2T, was isolated from a sample of orchard soil from Laizhou city, Shandong province, PR China. On the basis of 16S rRNA gene sequence analysis, strain LZ2T was closely related to members of the genus Sporosarcina, sharing highest levels of sequence similarity with Sporosarcina pasteurii NCIMB 8841T (98.8 %), Sporosarcina soli I80T (95.9 %). The value for the DNA-DNA relatedness between strain LZ2T and Sporosarcina pasteurii NCIMB 8841T was 39.8±1.7 %. Growth occurred at 10-44 °C (optimum, 30-35 °C), pH 5.0-11.0 (optimum pH 9.0-10.0); NaCl concentrations of up to 7.0 % (w/v) were tolerated. The dominant respiratory quinone was MK-7 and the G+C content was 39.2 mol%. The major fatty acids were anteiso-C15 : 0 and iso-C15 : 0. The major polar lipids of strain LZ2T were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid. Based on phenotypic and chemotaxonomic characteristics, and phylogenetic data strain LZ2T represents a novel species of the genus Sporosarcina, for which the name Sporosarcina terrae sp. nov. (type strain LZ2T=KACC 18822T=MCCC 1K03174T) is proposed.
authors.affiliation: Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, Institute of Microbiology, School of Life Science, Lanzhou University, Tianshui Road No.222, Lanzhou 730000, PR China.|Gansu Institute for Drug Control, Yinan Road No.7, Lanzhou 730070, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Grassland Agro-ecosystems, School of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Institute for Microbial and Biochemical Pharmacy, School of Pharmacy, Lanzhou University, Donggang Road No. 199, Lanzhou 730020, PR China.
authors.forename: Dejuan
authors.initials: D
authors.lastname: Zhi
authors.affiliation: Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, Institute of Microbiology, School of Life Science, Lanzhou University, Tianshui Road No.222, Lanzhou 730000, PR China.
authors.forename: Zining
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, Institute of Microbiology, School of Life Science, Lanzhou University, Tianshui Road No.222, Lanzhou 730000, PR China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, Institute of Microbiology, School of Life Science, Lanzhou University, Tianshui Road No.222, Lanzhou 730000, PR China.
authors.forename: Qinjian
authors.initials: Q
authors.lastname: Xie
authors.affiliation: Institute for Microbial and Biochemical Pharmacy, School of Pharmacy, Lanzhou University, Donggang Road No. 199, Lanzhou 730020, PR China.
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Institute for Microbial and Biochemical Pharmacy, School of Pharmacy, Lanzhou University, Donggang Road No. 199, Lanzhou 730020, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute for Microbial and Biochemical Pharmacy, School of Pharmacy, Lanzhou University, Donggang Road No. 199, Lanzhou 730020, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute for Microbial and Biochemical Pharmacy, School of Pharmacy, Lanzhou University, Donggang Road No. 199, Lanzhou 730020, PR China.
authors.forename: Lan
authors.initials: L
authors.lastname: Yu
authors.affiliation: Gansu Academy of Sciences, Lanzhou 730000, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.affiliation: Gansu Academy of Sciences, Lanzhou 730000, PR China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, Institute of Microbiology, School of Life Science, Lanzhou University, Tianshui Road No.222, Lanzhou 730000, PR China.|Institute for Microbial and Biochemical Pharmacy, School of Pharmacy, Lanzhou University, Donggang Road No. 199, Lanzhou 730020, PR China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Li
mesh_terms: D000383:Agriculture; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D058644:Sporosarcina / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7