title: Platelet Factor 4 Binds to Vascular Proteoglycans and Controls Both Growth Factor Activities and Platelet Activation.
abstract: Platelet factor 4 (PF4) is produced by platelets with roles in both inflammation and wound healing. PF4 is stored in platelet α-granules bound to the glycosaminoglycan (GAG) chains of serglycin. This study revealed that platelet serglycin is decorated with chondroitin/dermatan sulfate and that PF4 binds to these GAG chains. Additionally, PF4 had a higher affinity for endothelial-derived perlecan heparan sulfate chains than serglycin GAG chains. The binding of PF4 to perlecan was found to inhibit both FGF2 signaling and platelet activation. This study revealed additional insight into the ways in which PF4 interacts with components of the vasculature to modulate cellular events.
authors.affiliation: From the Graduate School of Biomedical Engineering, University of New South Wales, Sydney, New South Wales 2052, Australia and m.lord@unsw.edu.au.
authors.forename: Megan S
authors.initials: MS
authors.lastname: Lord
authors.affiliation: From the Graduate School of Biomedical Engineering, University of New South Wales, Sydney, New South Wales 2052, Australia and.
authors.forename: Bill
authors.initials: B
authors.lastname: Cheng
authors.affiliation: From the Graduate School of Biomedical Engineering, University of New South Wales, Sydney, New South Wales 2052, Australia and.
authors.forename: Brooke L
authors.initials: BL
authors.lastname: Farrugia
authors.affiliation: the Tricol Biomedical, Portland, Oregon 97205.
authors.forename: Simon
authors.initials: S
authors.lastname: McCarthy
authors.affiliation: From the Graduate School of Biomedical Engineering, University of New South Wales, Sydney, New South Wales 2052, Australia and.
authors.forename: John M
authors.initials: JM
authors.lastname: Whitelock
mesh_terms: D001792:Blood Platelets / Q000378:metabolism*; D015153:Blotting, Western; D002809:Chondroitin Sulfates / Q000378:metabolism*; D003871:Dermatan Sulfate / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D019812:Heparan Sulfate Proteoglycans / Q000378:metabolism*; D006497:Heparan Sulfate / Q000378:metabolism*; D006801:Humans; D015539:Platelet Activation; D010978:Platelet Factor 4 / Q000378:metabolism*; D011485:Protein Binding; D011509:Proteoglycans / Q000378:metabolism*; D033921:Vesicular Transport Proteins / Q000378:metabolism*
chemical_list: D002809:Chondroitin Sulfates; D003871:Dermatan Sulfate; D016222:Fibroblast Growth Factor 2; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D010978:Platelet Factor 4; D011509:Proteoglycans; D033921:Vesicular Transport Proteins; C075430:serglycin; C071927:perlecan
keywords: extracellular matrix protein; glycosaminoglycan; heparan sulfate; perlecan; proteoglycan; proteoglycan structure; serglycin