title: Regular and Moderate Exercise Counteracts the Decline of Antioxidant Protection but Not Methylglyoxal-Dependent Glycative Burden in the Ovary of Reproductively Aging Mice.
abstract: Population aging results in urgent needs of interventions aimed at ensuring healthy senescence. Exercise often results in healthy aging, yet many molecular mechanisms underlying such effects still need to be identified. We here investigated whether the age-dependent accumulation of oxidative and methylglyoxal- (MG-) related molecular damage could be delayed by moderate exercise in the mouse ovary, an organ that first exhibits impaired function with advancing age in mammals. CD1 female mice underwent two- or four-month treadmill-based running through the transition from adult to middle age, when ovaries show signs of senescence, and markers of protection against reactive oxygen species (ROS) and MG were measured. The long-term exercise reduced the protein oxidative damage in the ovaries (P< 0.01), and this was linked to the preservation of the glutathione peroxidase protection against ROS (P< 0.001), as well as to the increased glutathione availability (P< 0.001). Conversely, even though the age-related deactivation of the MG-targeting systems was partially prevented by the long-term running programme (P< 0.001), exercised mice were not protected from the age-dependent glycative burden. In summary, lately initiated regular and moderate exercise limited some changes occurring in the ovaries of middle-aged mice, and this might help to develop nonpharmacological cointerventions to reduce the vulnerability of mammalian ovaries towards redox dysfunctions.
authors.affiliation: Department of Life, Health and Environmental Sciences, University of L'Aquila, L'Aquila, Italy.
authors.forename: S
authors.identifier: 0000-0002-5978-0545
authors.initials: S
authors.lastname: Falone
authors.affiliation: Department of Life, Health and Environmental Sciences, University of L'Aquila, L'Aquila, Italy.
authors.forename: S Jr
authors.identifier: 0000-0001-6577-5892
authors.initials: SJ
authors.lastname: Santini
authors.affiliation: Department of Life, Health and Environmental Sciences, University of L'Aquila, L'Aquila, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Cordone
authors.affiliation: Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY, USA.
authors.forename: M
authors.initials: M
authors.lastname: Grannonico
authors.affiliation: Department of Biomedical Sciences, "G. d'Annunzio" University, Via dei Vestini, Chieti Scalo, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Cacchio
authors.affiliation: Department of Life, Health and Environmental Sciences, University of L'Aquila, L'Aquila, Italy.
authors.forename: G
authors.identifier: 0000-0001-9442-6923
authors.initials: G
authors.lastname: Di Emidio
authors.affiliation: Department of Life, Health and Environmental Sciences, University of L'Aquila, L'Aquila, Italy.
authors.forename: C
authors.identifier: 0000-0002-4978-2571
authors.initials: C
authors.lastname: Tatone
authors.affiliation: Department of Life, Health and Environmental Sciences, University of L'Aquila, L'Aquila, Italy; Institute of Translational Pharmacology (IFT), National Research Council (CNR), L'Aquila, Italy.
authors.forename: F
authors.identifier: 0000-0003-4528-4660
authors.initials: F
authors.lastname: Amicarelli
mesh_terms: D000375:Aging; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007791:Lactoylglutathione Lyase / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009952:Ornithine / Q000031:analogs & derivatives / Q000378:metabolism; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D010805:Physical Conditioning, Animal; D050050:Protein Carbonylation / Q000187:drug effects; D011743:Pyrimidines / Q000378:metabolism; D011765:Pyruvaldehyde / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012098:Reproduction / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D013869:Thiolester Hydrolases / Q000235:genetics / Q000378:metabolism; D000099301:Hydroxyacylglutathione Hydrolase
chemical_list: D000975:Antioxidants; D002374:Catalase; D005978:Glutathione; D005979:Glutathione Peroxidase; D007791:Lactoylglutathione Lyase; D009952:Ornithine; D011743:Pyrimidines; D011765:Pyruvaldehyde; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; D013869:Thiolester Hydrolases; D000099301:Hydroxyacylglutathione Hydrolase; C107313:argpyrimidine