title: Complete nucleotide sequence of clematis chlorotic mottle virus, a new member of the family Tombusviridae.
abstract: Clematis chlorotic mottle virus (ClCMV) is a previously undescribed virus associated with symptoms of yellow mottling and veining, chlorotic ring spots, line pattern mosaics, and flower distortion and discoloration on ornamental Clematis. The ClCMV genome is 3,880 nt in length with five open reading frames (ORFs) encoding a 27-kDa protein (ORF 1), an 87-kDa replicase protein (ORF 2), two centrally located movement proteins (ORF 3 and 4), and a 37-kDa capsid protein (ORF 5). Based on morphological, genomic, and phylogenetic analysis, ClCMV is predicted to be a member of the genus Pelarspovirus in the family Tombusviridae.
authors.affiliation: USDA ARS, National Germplasm Resources Laboratory, Beltsville, MD, 20705, USA.
authors.forename: Margaret
authors.initials: M
authors.lastname: McLaughlin
authors.affiliation: Department of Plant Pathology, University of Minnesota, St. Paul, MN, 55108, USA.
authors.forename: Ben
authors.initials: B
authors.lastname: Lockhart
authors.affiliation: USDA ARS, U.S. National Arboretum, Floral and Nursery Plant Research Unit, Beltsville, MD, 20705, USA.
authors.forename: Ramon
authors.initials: R
authors.lastname: Jordan
authors.affiliation: Royal Horticultural Society, Woking, Surrey, GU23 6QB, UK.
authors.forename: Geoff
authors.initials: G
authors.lastname: Denton
authors.affiliation: USDA ARS, National Germplasm Resources Laboratory, Beltsville, MD, 20705, USA. dimitre.mollov@ars.usda.gov.
authors.forename: Dimitre
authors.identifier: http://orcid.org/0000-0002-4750-6194
authors.initials: D
authors.lastname: Mollov
mesh_terms: D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics*; D039324:Clematis / Q000821:virology*; D016679:Genome, Viral; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*; D017423:Sequence Analysis, RNA; D019183:Tombusviridae / Q000145:classification* / Q000235:genetics*
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase