title: Assessment of the Antioxidant Activity ofSilybum marianumSeed Extract and Its Protective Effect against DNA Oxidation, Protein Damage and Lipid Peroxidation.
abstract: Antioxidant properties of ethanol extract ofSilybum marianum(milk thistle) seeds was investigated. We have also investigated the protein damage activated by oxidative Fenton reaction and its prevention bySilybum marianumseed extract. Antioxidant potential ofSilybum marianumseed ethanol extract was measured using differentin vitromethods, such as lipid peroxidation, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing power assays. The extract significantly decreased DNA damage caused by hydroxyl radicals. Protein damage induced by hydroxyl radicals was also efficiently inhibited, which was confirmed by the presence of protein damage markers, such as protein carbonyl formation and by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The present study shows that milk thistle seeds have good DPPH free radical scavenging activity and can prevent lipid peroxidation. Therefore,Silybum marianumcan be used as potentially rich source of antioxidants and food preservatives. The results suggest that the seeds may have potential beneficial health effects providing opportunities to develop value-added products.
authors.affiliation: Chemistry Department, Science Faculty, Dicle University, TR-21280 Diyarbakır, Turkey.
authors.forename: Aynur
authors.initials: A
authors.lastname: Serçe
authors.affiliation: Chemistry Department, Science Faculty, Dicle University, TR-21280 Diyarbakır, Turkey.
authors.forename: Bircan Çeken
authors.initials: BÇ
authors.lastname: Toptancı
authors.affiliation: Chemistry Department, Science Faculty, Dicle University, TR-21280 Diyarbakır, Turkey.
authors.forename: Sevil Emen
authors.initials: SE
authors.lastname: Tanrıkut
authors.affiliation: Chemistry Department, Science Faculty, Dicle University, TR-21280 Diyarbakır, Turkey.
authors.forename: Sevcan
authors.initials: S
authors.lastname: Altaş
authors.affiliation: Chemistry Department, Science Faculty, Dicle University, TR-21280 Diyarbakır, Turkey.
authors.forename: Göksel
authors.initials: G
authors.lastname: Kızıl
authors.affiliation: Department of Field Crops, Faculty of Agriculture, Dicle University, TR-21280 Diyarbakır, Turkey.
authors.forename: Süleyman
authors.initials: S
authors.lastname: Kızıl
authors.affiliation: Chemistry Department, Science Faculty, Dicle University, TR-21280 Diyarbakır, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Kızıl
keywords: SDS–PAGE; Silybum marianum (milk thistle) seeds; antioxidant activity; carbonyl formation; lipid peroxidation; oxidative DNA damage; protein oxidation; radical scavenging activity