title: Fermented green tea extract exhibits hypolipidaemic effects through the inhibition of pancreatic lipase and promotion of energy expenditure.
abstract: Hyperlipidaemia is a major cause of atherosclerosis and related CVD and can be prevented with natural substances. Previously, we reported that a novel Bacillus-fermented green tea (FGT) exerts anti-obesity and hypolipidaemic effects. This study further investigated the hypotriglyceridaemic and anti-obesogenic effects of FGT and its underlying mechanisms. FGT effectively inhibited pancreatic lipase activity in vitro (IC50, 0·48 mg/ml) and ameliorated postprandial lipaemia in rats (26 % reduction with 500 mg/kg FGT). In hypertriglyceridaemic hamsters, FGT administration significantly reduced plasma TAG levels. In mice, FGT administration (500 mg/kg) for 2 weeks augmented energy expenditure by 22 % through the induction of plasma serotonin, a neurotransmitter that modulates energy expenditure and mRNA expressions of lipid metabolism genes in peripheral tissues. Analysis of the gut microbiota showed that FGT reduced the proportion of the phylum Firmicutes in hamsters, which could further contribute to its anti-obesity effects. Collectively, these data demonstrate that FGT decreases plasma TAG levels via multiple mechanisms including inhibition of pancreatic lipase, augmentation of energy expenditure, induction of serotonin secretion and alteration of gut microbiota. These results suggest that FGT may be a useful natural agent for preventing hypertriglyceridaemia and obesity.
authors.affiliation: 1Department of Biotechnology,BK21-PLUS,College of Life Sciences & Biotechnology,Korea University,Seoul 02841,Republic of Korea.
authors.forename: Dae-Bang
authors.initials: DB
authors.lastname: Seo
authors.affiliation: 2Beauty Food Division,Amorepacific Corporation R&D Center,Gyeonggi-Do 17074,Republic of Korea.
authors.forename: Hyun Woo
authors.initials: HW
authors.lastname: Jeong
authors.affiliation: 1Department of Biotechnology,BK21-PLUS,College of Life Sciences & Biotechnology,Korea University,Seoul 02841,Republic of Korea.
authors.forename: Yeon-Ji
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: 2Beauty Food Division,Amorepacific Corporation R&D Center,Gyeonggi-Do 17074,Republic of Korea.
authors.forename: Sukyung
authors.initials: S
authors.lastname: Kim
authors.affiliation: 2Beauty Food Division,Amorepacific Corporation R&D Center,Gyeonggi-Do 17074,Republic of Korea.
authors.forename: Jeongkee
authors.initials: J
authors.lastname: Kim
authors.affiliation: 3Medical Beauty Division,Amorepacific Corporation R&D Center,Gyeonggi-Do 17074,Republic of Korea.
authors.forename: Ji Hae
authors.initials: JH
authors.lastname: Lee
authors.affiliation: 4Skin Research Division,Amorepacific Corporation R&D Center,Gyeonggi-Do 17074,Republic of Korea.
authors.forename: Kyungmi
authors.initials: K
authors.lastname: Joo
authors.affiliation: 5QC team,Aeustra Corporation,Gyeonggi-Do 17573,Republic of Korea.
authors.forename: Jin Kyu
authors.initials: JK
authors.lastname: Choi
authors.affiliation: 2Beauty Food Division,Amorepacific Corporation R&D Center,Gyeonggi-Do 17074,Republic of Korea.
authors.forename: Song Seok
authors.initials: SS
authors.lastname: Shin
authors.affiliation: 1Department of Biotechnology,BK21-PLUS,College of Life Sciences & Biotechnology,Korea University,Seoul 02841,Republic of Korea.
authors.forename: Sung-Joon
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology / Q000627:therapeutic use; D001407:Bacillus; D028241:Camellia sinensis; D004734:Energy Metabolism / Q000187:drug effects*; D005285:Fermentation; D000068536:Bacillota; D000069196:Gastrointestinal Microbiome / Q000187:drug effects; D006949:Hyperlipidemias / Q000097:blood* / Q000188:drug therapy; D015228:Hypertriglyceridemia / Q000097:blood / Q000188:drug therapy; D000960:Hypolipidemic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D008049:Lipase / Q000037:antagonists & inhibitors*; D050356:Lipid Metabolism / Q000187:drug effects / Q000235:genetics; D008647:Mesocricetus; D051379:Mice; D008810:Mice, Inbred C57BL; D010179:Pancreas / Q000201:enzymology; D008517:Phytotherapy; D010936:Plant Extracts / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D012333:RNA, Messenger / Q000378:metabolism; D012701:Serotonin / Q000097:blood; D013662:Tea; D014280:Triglycerides / Q000097:blood
chemical_list: D019440:Anti-Obesity Agents; D000960:Hypolipidemic Agents; D010936:Plant Extracts; D012333:RNA, Messenger; D013662:Tea; D014280:Triglycerides; D012701:Serotonin; D008049:Lipase
keywords:  AMPK AMP-activated protein kinase;  FF fenofibrate;  FGT fermented green tea;  HFD high-fat diet;  LPL lipoprotein lipase; Energy expenditure; Fermented green tea; Gut microbiota; Hyperlipidaemia; Pancreatic lipase; Serotonin