title: Fournierella massiliensis gen. nov., sp. nov., a new human-associated member of the family Ruminococcaceae.
abstract: An anaerobic bacterium, strain AT2T, was isolated from the fresh stool sample of a healthy French man using the culturomics approach. The 16S rRNA gene sequence analysis showed that strain AT2T had 95.2 % nucleotide sequence similarity with Gemmiger formicilisATCC 27749T, the phylogenetically closest species with standing in nomenclature. Cells are Gram-stain-negative, catalase- and oxidase-negative, obligately anaerobic, non-motile, non-spore-forming, rod-shaped, and the bacilli were mesothermophilic. The major fatty acids were C16 : 0 (43.8 %) and C18 : 1n9 (20 %). The DNA G+C content of the strain based on its genome sequence was 56.8 mol%. Based on the phenotypic, biochemical and phylogenetic analysis, we propose the creation of the genus Fournierella gen. nov., which contains strain AT2T (=CSUR P2014T=DSM 100451T) as the type strain of the type species Fournierella massiliensis gen. nov., sp. nov.
authors.affiliation: URMITE, UM63, CNRS7278, IRD198, INSERM1095, Institut Hospitalier Universitaire Méditerranée-Infection, Aix Marseille Université, Marseille, France.
authors.forename: Amadou Hamidou
authors.initials: AH
authors.lastname: Togo
authors.affiliation: URMITE, UM63, CNRS7278, IRD198, INSERM1095, Institut Hospitalier Universitaire Méditerranée-Infection, Aix Marseille Université, Marseille, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Durand
authors.affiliation: URMITE, UM63, CNRS7278, IRD198, INSERM1095, Institut Hospitalier Universitaire Méditerranée-Infection, Aix Marseille Université, Marseille, France.
authors.forename: Saber
authors.initials: S
authors.lastname: Khelaifia
authors.affiliation: URMITE, UM63, CNRS7278, IRD198, INSERM1095, Institut Hospitalier Universitaire Méditerranée-Infection, Aix Marseille Université, Marseille, France.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Armstrong
authors.affiliation: URMITE, UM63, CNRS7278, IRD198, INSERM1095, Institut Hospitalier Universitaire Méditerranée-Infection, Aix Marseille Université, Marseille, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Robert
authors.affiliation: URMITE, UM63, CNRS7278, IRD198, INSERM1095, Institut Hospitalier Universitaire Méditerranée-Infection, Aix Marseille Université, Marseille, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Cadoret
authors.affiliation: URMITE, UM63, CNRS7278, IRD198, INSERM1095, Institut Hospitalier Universitaire Méditerranée-Infection, Aix Marseille Université, Marseille, France.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Di Pinto
authors.affiliation: URMITE, UM63, CNRS7278, IRD198, INSERM1095, Institut Hospitalier Universitaire Méditerranée-Infection, Aix Marseille Université, Marseille, France.
authors.forename: Jérémy
authors.initials: J
authors.lastname: Delerce
authors.affiliation: URMITE, UM63, CNRS7278, IRD198, INSERM1095, Institut Hospitalier Universitaire Méditerranée-Infection, Aix Marseille Université, Marseille, France.
authors.forename: Anthony
authors.initials: A
authors.lastname: Levasseur
authors.affiliation: URMITE, UM63, CNRS7278, IRD198, INSERM1095, Institut Hospitalier Universitaire Méditerranée-Infection, Aix Marseille Université, Marseille, France.|Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Didier
authors.initials: D
authors.lastname: Raoult
authors.affiliation: URMITE, UM63, CNRS7278, IRD198, INSERM1095, Institut Hospitalier Universitaire Méditerranée-Infection, Aix Marseille Université, Marseille, France.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Million
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005243:Feces / Q000382:microbiology*; D005602:France; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008297:Male; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S