title: Detection of Reactive Oxygen and Nitrogen Species by Electron Paramagnetic Resonance (EPR) Technique.
abstract: During the last decade there has been growing interest in physical-chemical oxidation processes and the behavior of free radicals in living systems. Radicals are known as intermediate species in a variety of biochemical reactions. Numerous techniques, assays and biomarkers have been used to measure reactive oxygen and nitrogen species (ROS and RNS), and to examine oxidative stress. However, many of these assays are not entirely satisfactory or are used inappropriately. The purpose of this chapter is to review current EPR (Electron Paramagnetic Resonance) spectroscopy methods for measuring ROS, RNS, and their secondary products, and to discuss the strengths and limitations of specific methodological approaches.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Ankara University, Tandogan, Ankara 06100, Turkey. sibel@pharmacy.ankara.edu.tr.
authors.forename: Sibel
authors.initials: S
authors.lastname: Suzen
authors.affiliation: Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Ege University, 35100 Izmir, Turkey. hgurer@gmail.com.
authors.forename: Hande
authors.initials: H
authors.lastname: Gurer-Orhan
authors.affiliation: Department of Physiology and Pharmacology "Vittorio Erspamer", Sapienza University of Rome, P. le Aldo Moro 5, 00185 Rome, Italy. luciano.saso@uniroma1.it.
authors.forename: Luciano
authors.initials: L
authors.lastname: Saso
mesh_terms: D000818:Animals; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D005609:Free Radicals / Q000737:chemistry; D006801:Humans; D018384:Oxidative Stress; D026361:Reactive Nitrogen Species / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000737:chemistry*
chemical_list: D005609:Free Radicals; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species
keywords: EPR; RNS; ROS; free radical; oxidative stress