title: Azohydromonas riparia sp. nov. and Azohydromonas ureilytica sp. nov. isolated from a riverside soil in South Korea.
abstract: White and pale yellow coloured bacteria were isolated from the riverside soil, Daejeon, South Korea, and were designated UCM-11T, UCM-F25, and UCM-80T. We found that all strains were able to reduce nitrate, and the cells were aerobic and motile. The DNA G+C contents of UCM-11T, UCM-F25, and UCM-80Twere between 68.9 to 71.2 mol% and the main ubiquinone was observed as Q-8. Based on16S rRNA gene sequences, strains UCM-11Tand UCM-F25 were found to closely match with Azohydromonas australica IAM 12664T(98.48-98.55%), and the strain UCM-80Twas the closest match with Azohydromonas lata IAM 12599T(98.34%). The presence of summed feature 3 (C16:1ω7c and/or C16:1ω6c), C16:0, summed feature 8 (C18:1ω7c and/or C18:1ω6c) as well as twokinds of hydroxyfatty acids consisting of C10:03-OH and C12:02-OH, and branched fatty acids containing C16:0iso and C17:0cyclo were detected in all the strains. Phosphatidylethanolamine was a major polar lipid. DNA-DNA relatedness confirmed UCM-11T, UCM-F25 and UCM-80Tas novel members of the genus Azohydromonas. Based on the morphological, physiological, biochemical and genotypic characteristics, we suggest that strains UCM-11T, UCM-F25, and UCM-80Trepresent novel species within the genus Azohydromonas. The names Azohydromonas riparia sp. nov., and Azohydromonas ureilytica sp. nov. are proposed for the type strains UCM-11T(=KACC 18570T=NBRC 111646T) and UCM-80T(=KACC 18576T=NBRC 111658T), respectively.
authors.affiliation: Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, 16227, Republic of Korea.|Thai Nguyen University of Agriculture and Forestry, Quyet Thang Commune, Thai Nguyen City, Vietnam.
authors.forename: Tuan Manh
authors.initials: TM
authors.lastname: Nguyen
authors.affiliation: Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, 16227, Republic of Korea. jkimtamu@kgu.ac.kr.
authors.forename: Jaisoo
authors.initials: J
authors.lastname: Kim
mesh_terms: D042401:Alcaligenaceae / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal; D005227:Fatty Acids / Q000032:analysis; D010714:Phosphatidylethanolamines; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; C483858:phosphatidylethanolamine
keywords: Azohydromonas riparia sp. nov.; Azohydromonas ureilytica sp. nov.; Proteobacteria; soil; taxonomy