title: Urine Metabolomics in Hypertension Research.
abstract: Functional genomics requires an understanding of the complete network of changes within an organism by extensive measurements of moieties from mRNA, proteins, and metabolites. Metabolomics utilizes analytic chemistry tools to profile the complete spectrum of metabolites found in a tissue, cells, or biofluids using a wide range of tools from infrared spectroscopy, fluorescence spectroscopy, NMR spectroscopy, and mass spectrometry. In this protocol, we outline a procedure for performing metabolomic analysis of urine samples using liquid chromatography-mass spectrometry (LC-MS). We outline the advantages of using this approach and summarize some of the early promising studies in cardiovascular diseases using this approach.
authors.affiliation: Institute of Cardiovascular and Medical Sciences, BHF Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, UK. s.tsiropoulou.1@research.gla.ac.uk.
authors.forename: Sofia
authors.initials: S
authors.lastname: Tsiropoulou
authors.affiliation: Institute of Cardiovascular and Medical Sciences, BHF Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, UK.
authors.forename: Martin
authors.initials: M
authors.lastname: McBride
authors.affiliation: Institute of Cardiovascular and Medical Sciences, BHF Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, UK.
authors.forename: Sandosh
authors.initials: S
authors.lastname: Padmanabhan
mesh_terms: D002318:Cardiovascular Diseases / Q000235:genetics / Q000378:metabolism / Q000652:urine; D006973:Hypertension / Q000235:genetics / Q000378:metabolism* / Q000652:urine*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D055432:Metabolomics / Q000379:methods*; D013050:Spectrometry, Fluorescence
keywords: Hypertension; Mass spectrometry; Metabolomics; Urine