title: A Statistical Evaluation of Methods of In-Vitro Growth Assessment for Phyllosticta citricarpa: Average Colony Diameter vs. Area.
abstract: Fungal growth inhibition on solid media has been historically measured and calculated based on the average of perpendicular diameter measurements of growth on fungicide amended media. We investigated the sensitivity of the calculated area (DA) and the measured area (MA) for assessing fungicide growth inhibition of the ascomycete, Phyllosticta citricarpa on solid media. Both the calculated, DA and the actual measured area, MA were adequate for distinguishing significant treatment effects of fungicide on fungal growth, however MA was more sensitive at identifying significant differences between the controls and fungicide concentrations below 5 ppm.
authors.affiliation: Department of Plant Pathology, University of Florida, Immokalee, Florida, United States of America.
authors.forename: Katherine E
authors.identifier: http://orcid.org/0000-0003-1125-4658
authors.initials: KE
authors.lastname: Hendricks
authors.affiliation: Department of Statistics, University of Florida, Gainesville, Florida, United States of America.
authors.forename: Mary C
authors.initials: MC
authors.lastname: Christman
authors.affiliation: Department of Plant Pathology, University of Florida, Immokalee, Florida, United States of America.
authors.forename: Pamela D
authors.initials: PD
authors.lastname: Roberts
mesh_terms: D001203:Ascomycota / Q000187:drug effects* / Q000254:growth & development; D003470:Culture Media / Q000494:pharmacology; D052978:Disk Diffusion Antimicrobial Tests / Q000706:statistics & numerical data*; D005659:Fungicides, Industrial / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D005659:Fungicides, Industrial