title: SESN2 correlates with advantageous prognosis in hepatocellular carcinoma.
abstract: BACKGROUND
  SESN2 plays important roles in the regulation of cell survival, cell protection, and tumor suppression. However, the relationship between SESN2 expression and the clinicopathological attributes of hepatocellular carcinoma (HCC) is barely investigated.
  
  
  METHODS
  One-step quantitative reverse transcription PCR, Western blotting analysis in 15 fresh HCC tissues, and immunohistochemistry (IHC) analysis in a tissue microarray (TMA) containing 100 HCC cases were performed to examine SESN2 expression. Survival analyses by Cox regression method and Kaplan-Meier curve were performed to describe the overall survival of 100 HCC patients.
  
  
  RESULTS
  The SESN2 expression in HCC tissues declined dramatically compared with the corresponding noncancerous tissues, and SESN2 expression was remarkably associated with HBV infection (p = 0.019), HCV infection (p = 0.001), and lymph node metastasis (p = 0.033). Survival analysis further demonstrated that SESN2 expression could serve as an independent prognostic biomarker for overall survival in univariate (p = 0.001) and multivariate analyses (p = 0.003).
  
  
  CONCLUSIONS
  The data are the first to indicate that SESN2 might be a novel prognostic marker for HCC and that elevated SESN2 expression predicts advantageous outcomes in HCC patients.
authors.affiliation: Department of Thoracic Surgery and Oncology, the No. 2 Affiliated Hospital of Qiqhar Medical University, Qiqhar, 161000, China.
authors.forename: Shaosen
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Oncology, the First Hospital of Qiqhar City, Qiqhar, 161000, China.
authors.forename: Weigang
authors.initials: W
authors.lastname: Yan
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences of Qiqhar Medical University, Qiqhar, 161000, China.
authors.forename: Weiya
authors.initials: W
authors.lastname: Lang
authors.affiliation: Department of Histology and Embryology, School of Basic Medical Sciences of Qiqhar Medical University, Qiqhar, 161000, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Pathology, Jiangsu Cancer Hospital, Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, 210000, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Pathology, Jiangsu Cancer Hospital, Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, 210000, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Thoracic Surgery and Oncology, the No. 2 Affiliated Hospital of Qiqhar Medical University, Qiqhar, 161000, China. lylqqhe@126.com.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Thoracic Surgery and Oncology, the No. 2 Affiliated Hospital of Qiqhar Medical University, Qiqhar, 161000, China. bhg28k@163.com.
authors.forename: Hongguang
authors.initials: H
authors.lastname: Bao
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000401:mortality / Q000473:pathology*; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000401:mortality / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000032:analysis / Q000096:biosynthesis*; D011379:Prognosis; D016016:Proportional Hazards Models; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046888:Tissue Array Analysis; D000084622:Sestrins
chemical_list: D014408:Biomarkers, Tumor; D009687:Nuclear Proteins; C465398:SESN2 protein, human; D000084622:Sestrins
keywords: Hepatocellular carcinoma; IHC; SESN2; Western blotting; qPCR