title: A new compound, methylbergenin along with eight known compounds with cytotoxicity and anti-inflammatory activity from Ardisia japonica.
abstract: A new compound, methylbergenin (1), was isolated from the whole plants of Ardisia japonica (Thunb.) Bl. (Myrsinaceae), along with eight known compounds: demethoxybergenin (2), bergenin (3), afzelin (4), quercitrin (5), bauerenol (6), bauerenone (7), α-spinasterol (8) and chondrillasterone (9). Compounds 1, 7 and 9 were isolated from A. japonica for the first time. The structure of compound 1 was determined on the basis of NMR (1D and 2D) and mass spectroscopic analysis. The absolute configuration of 1 was assessed by single-crystal X-ray diffraction. Compounds 1, 4, 5, 7 and 9 showed potential inhibitory effects against nitric oxide production in LPS stimulated RAW 264.7 murine macrophages. In addition, Compounds 7 and 9 exhibited cytotoxicity against A549 and HepG-2 cells.
authors.affiliation: a State Key Laboratory of Natural Medicines , China Pharmaceutical University , Nanjing , China.
authors.forename: Ke-Yun
authors.initials: KY
authors.lastname: Yu
authors.affiliation: b College of Pharmacy , Guilin Medical University , Guilin , China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: a State Key Laboratory of Natural Medicines , China Pharmaceutical University , Nanjing , China.
authors.forename: Shang-Zhen
authors.initials: SZ
authors.lastname: Li
authors.affiliation: a State Key Laboratory of Natural Medicines , China Pharmaceutical University , Nanjing , China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Dou
authors.affiliation: a State Key Laboratory of Natural Medicines , China Pharmaceutical University , Nanjing , China.
authors.forename: Le-Le
authors.initials: LL
authors.lastname: Liu
authors.affiliation: a State Key Laboratory of Natural Medicines , China Pharmaceutical University , Nanjing , China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: a State Key Laboratory of Natural Medicines , China Pharmaceutical University , Nanjing , China.
authors.forename: E-Hu
authors.initials: EH
authors.lastname: Liu
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry* / Q000494:pharmacology*; D031644:Ardisia / Q000737:chemistry*; D001578:Benzopyrans / Q000302:isolation & purification; D018360:Crystallography, X-Ray; D004353:Drug Evaluation, Preclinical / Q000379:methods; D056945:Hep G2 Cells; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000096:biosynthesis; D013265:Stigmasterol / Q000031:analogs & derivatives / Q000302:isolation & purification; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D001578:Benzopyrans; D008070:Lipopolysaccharides; D014315:Triterpenes; C031312:spinasterol; D009569:Nitric Oxide; C061436:bauerenol; D013265:Stigmasterol; C006741:bergenin
keywords: Ardisia japonica; cytotoxicity; inhibition of NO production