title: Serum concentrations of apoptosis-associated molecules in septic children with leukemia, neutropenia and fever.
abstract: It has been shown that Fas, Fas-L, TNF and TNFR-1 display high serum concentrations in subjects with sepsis. This suggests that these are potential severity markers. However, the serum concentration of these molecules in children with leukemia and suspected sepsis has to be established before proposing their use as diagnostic biomarkers. We included children <17 years of age diagnosed with acute lymphoblastic leukemia with neutropenia and fever (NF). The subjects were divided into two groups: (1) leukemia and NF with sepsis, (2) leukemia and NF without sepsis. Determination of serum levels of TNF-α, TNFR-1, Fas and Fas-L was performed using ELISA tests, and apoptosis percentage using flow cytometry. Seventy-two subjects with ALL and NF were included in the two groups. The highest serum levels of TNF-α (35.2 ± 7.6 pg/ml) and TNF-R1 (4102 ± 2440) and the lowest levels of Fas-L (19.4 ± 7.3 pg/ml) were found in group 2: however, the difference in comparison with patients without sepsis was not statistically significant. Low levels of Fas-L and low percentage of apoptotic cells are observed in septic subjects. This pattern may reflect the presence of sepsis among subjects with NF secondary to leukemia.
authors.affiliation: Pediatrics Service, South Central Hospital of High Specialty of Petroleos Mexicanos, Health Services of Petroleos Mexicanos, Dirección: Blvd. Adolfo Ruiz Cortines 4091, Tlalpan, Fuentes del Pedregal, 14140, Mexico City, DF, Mexico. jesusreynaf@gmail.com.|Centre for Research on Infectious Diseases, National Institute of Public Health, Secretary of Health, Cuernavaca, Morelos, Mexico. jesusreynaf@gmail.com.
authors.forename: Jesus
authors.initials: J
authors.lastname: Reyna-Figueroa
authors.affiliation: Centre for Research on Infectious Diseases, National Institute of Public Health, Secretary of Health, Cuernavaca, Morelos, Mexico.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Lagunas-Martínez
authors.affiliation: Pediatrics Service, South Central Hospital of High Specialty of Petroleos Mexicanos, Health Services of Petroleos Mexicanos, Dirección: Blvd. Adolfo Ruiz Cortines 4091, Tlalpan, Fuentes del Pedregal, 14140, Mexico City, DF, Mexico.
authors.forename: Patricia
authors.initials: P
authors.lastname: Galindo-Delgado
authors.affiliation: Pediatrics Service, South Central Hospital of High Specialty of Petroleos Mexicanos, Health Services of Petroleos Mexicanos, Dirección: Blvd. Adolfo Ruiz Cortines 4091, Tlalpan, Fuentes del Pedregal, 14140, Mexico City, DF, Mexico.
authors.forename: María Fernanda
authors.initials: MF
authors.lastname: Fernández-Bautista
authors.affiliation: Pediatrics Service, South Central Hospital of High Specialty of Petroleos Mexicanos, Health Services of Petroleos Mexicanos, Dirección: Blvd. Adolfo Ruiz Cortines 4091, Tlalpan, Fuentes del Pedregal, 14140, Mexico City, DF, Mexico.
authors.forename: Paola Guadalupe
authors.initials: PG
authors.lastname: Castro-Oteo
authors.affiliation: Pediatrics Service, South Central Hospital of High Specialty of Petroleos Mexicanos, Health Services of Petroleos Mexicanos, Dirección: Blvd. Adolfo Ruiz Cortines 4091, Tlalpan, Fuentes del Pedregal, 14140, Mexico City, DF, Mexico.
authors.forename: Pilar
authors.initials: P
authors.lastname: Martínez-Matsumoto
authors.affiliation: Department of Molecular Medicine and Bioprocesses, Institute of Biotechnology, National Autonomous University of Mexico, Mexico City, Mexico.
authors.forename: Erika Melchy
authors.initials: EM
authors.lastname: Perez
authors.affiliation: Department of Molecular Medicine and Bioprocesses, Institute of Biotechnology, National Autonomous University of Mexico, Mexico City, Mexico.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Rosenstein
authors.affiliation: Pediatrics Service, South Central Hospital of High Specialty of Petroleos Mexicanos, Health Services of Petroleos Mexicanos, Dirección: Blvd. Adolfo Ruiz Cortines 4091, Tlalpan, Fuentes del Pedregal, 14140, Mexico City, DF, Mexico.
authors.forename: Ana Elena
authors.initials: AE
authors.lastname: Limón-Rojas
authors.affiliation: Paediatric Infectology Service, Hospital Angeles del Pedregal, Magdalena Contreras, Mexico.
authors.forename: Federico Javier
authors.initials: FJ
authors.lastname: Ortiz-Ibarra
authors.affiliation: Centre for Research on Infectious Diseases, National Institute of Public Health, Secretary of Health, Cuernavaca, Morelos, Mexico.
authors.forename: Vicente
authors.initials: V
authors.lastname: Madrid-Marina
mesh_terms: D000293:Adolescent; D015415:Biomarkers / Q000097:blood; D002648:Child; D053222:Fas Ligand Protein / Q000097:blood*; D005260:Female; D005334:Fever / Q000209:etiology*; D006801:Humans; D008297:Male; D009503:Neutropenia / Q000209:etiology*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000150:complications* / Q000175:diagnosis*; D011446:Prospective Studies; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000097:blood*; D018805:Sepsis / Q000175:diagnosis* / Q000209:etiology*; D012720:Severity of Illness Index; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*; D019014:fas Receptor / Q000097:blood*
chemical_list: D015415:Biomarkers; C491901:FAS protein, human; D053222:Fas Ligand Protein; D047888:Receptors, Tumor Necrosis Factor, Type I; D014409:Tumor Necrosis Factor-alpha; D019014:fas Receptor
keywords: Apoptosis; Fas; Fas-L; Leukemia; Neutropenia and fever; Sepsis; TNF-R1; TNF-α