title: Early suppression of adipocyte lipid turnover induces immunometabolic modulation in cancer cachexia syndrome.
abstract: Cancer cachexia is a multifactorial syndrome characterized by body weight loss, atrophy of adipose tissue (AT) and systemic inflammation. However, there is limited information regarding the mechanisms of immunometabolic response in AT from cancer cachexia. Male Wistar rats were inoculated with 2 × 107of Walker 256 tumor cells [tumor bearing (TB) rats]. The mesenteric AT (MeAT) was collected on d 0, 4, 7 (early stage), and 14 (cachexia stage) after tumor cell injection. Surgical biopsies for MeAT were obtained from patients who had gastrointestinal cancer with cachexia. Lipolysis showed an early decrease in glycerol release in TB d 4 (TB4) rats in relation to the control, followed by a 6-fold increase in TB14 rats, whereasde novolipogenesis was markedly lower in the incorporation of glucose into fatty acids in TB14 rats during the development of cachexia. CD11b and CD68 were positive in TB7 and TB14 rats, respectively. In addition, we found cachexia stage results similar to those of animals in MeAT from patients: an increased presence of CD68+, iNOS2+, TNFα+, and HSL+cells. In summary, translational analysis of MeAT from patients and an animal model of cancer cachexia enabled us to identify early disruption in Adl turnover and subsequent inflammatory response during the development of cancer cachexia.-Henriques, F. S., Sertié, R. A. L., Franco, F. O., Knobl, P., Neves, R. X., Andreotti, S., Lima, F. B., Guilherme, A., Seelaender, M., Batista, M. L., Jr. Early suppression of adipocyte lipid turnover induces immunometabolic modulation in cancer cachexia syndrome.
authors.affiliation: Laboratory of Adipose Tissue Biology, Integrated Group of Biotechnology, University of Mogi das Cruzes, Mogi das Cruzes, São Paulo, Brazil.|Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
authors.forename: Felipe Santos
authors.initials: FS
authors.lastname: Henriques
authors.affiliation: Laboratory of Physiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, São Paulo, Brazil; and.
authors.forename: Rogério Antônio Laurato
authors.initials: RAL
authors.lastname: Sertié
authors.affiliation: Laboratory of Adipose Tissue Biology, Integrated Group of Biotechnology, University of Mogi das Cruzes, Mogi das Cruzes, São Paulo, Brazil.
authors.forename: Felipe Oliveira
authors.initials: FO
authors.lastname: Franco
authors.affiliation: Laboratory of Adipose Tissue Biology, Integrated Group of Biotechnology, University of Mogi das Cruzes, Mogi das Cruzes, São Paulo, Brazil.
authors.forename: Pamela
authors.initials: P
authors.lastname: Knobl
authors.affiliation: Cancer Metabolism Research Group, Institute of Biomedical Sciences, University of São Paulo, São Paulo, São Paulo, Brazil.
authors.forename: Rodrigo Xavier
authors.initials: RX
authors.lastname: Neves
authors.affiliation: Laboratory of Physiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, São Paulo, Brazil; and.
authors.forename: Sandra
authors.initials: S
authors.lastname: Andreotti
authors.affiliation: Laboratory of Physiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, São Paulo, Brazil; and.
authors.forename: Fabio Bessa
authors.initials: FB
authors.lastname: Lima
authors.affiliation: Laboratory of Physiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, São Paulo, Brazil; and.
authors.forename: Adilson
authors.initials: A
authors.lastname: Guilherme
authors.affiliation: Cancer Metabolism Research Group, Institute of Biomedical Sciences, University of São Paulo, São Paulo, São Paulo, Brazil.
authors.forename: Marilia
authors.initials: M
authors.lastname: Seelaender
authors.affiliation: Laboratory of Adipose Tissue Biology, Integrated Group of Biotechnology, University of Mogi das Cruzes, Mogi das Cruzes, São Paulo, Brazil; migueljr4@me.com.
authors.forename: Miguel Luiz
authors.initials: ML
authors.lastname: Batista
mesh_terms: D017667:Adipocytes / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002100:Cachexia / Q000209:etiology* / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism; D050356:Lipid Metabolism / Q000502:physiology; D008297:Male; D009369:Neoplasms / Q000150:complications* / Q000378:metabolism*; D017208:Rats, Wistar
chemical_list: D005227:Fatty Acids
keywords:  de novo lipogenesis; adipose tissue; inflammation; lipolysis