title: Torulene and torularhodin, protects human prostate stromal cells from hydrogen peroxide-induced oxidative stress damage through the regulation of Bcl-2/Bax mediated apoptosis.
abstract: The current study was designed to elucidate the cytoprotective effects and possible mechanisms of torulene and torularhodin on hydrogen peroxide (H2O2)-induced oxidative stress damage in human prostate stromal cells (WPMY-1). After treated with H2O2, a notable decrease was appeared in cell viability, yet the decrease was attenuated when cells were pretreated with torulene and torularhodin (0.5-10 μM) as evaluated by WST-1 assay. Pretreatment with these two carotenoids significantly attenuated H2O2-induced apoptosis in WPMY-1 cells through the inhibition of intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) overproduction, as well as the activation of the activities in catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px). Finally, pretreatment of cells with carotenoids resulted in the regulation of the mRNA and protein expression of Bcl-2 and Bax in H2O2-exposed prostate stromal cells. The present results indicate that both torulene and torularhodin can protect human prostate stromal cells from oxidative stress damage via Bcl-2/Bax mediated apoptosis.
authors.affiliation: a School of Food Science and Technology, Jiangnan University , Wuxi , Jiangsu Province , PR China.|b School of Biotechnology, Jiangnan University , Wuxi , Jiangsu Province , PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Du
authors.affiliation: a School of Food Science and Technology, Jiangnan University , Wuxi , Jiangsu Province , PR China.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Guo
authors.affiliation: a School of Food Science and Technology, Jiangnan University , Wuxi , Jiangsu Province , PR China.
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: b School of Biotechnology, Jiangnan University , Wuxi , Jiangsu Province , PR China.
authors.forename: Mei
authors.initials: M
authors.lastname: Han
authors.affiliation: b School of Biotechnology, Jiangnan University , Wuxi , Jiangsu Province , PR China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Zhang
authors.affiliation: a School of Food Science and Technology, Jiangnan University , Wuxi , Jiangsu Province , PR China.|c National Engineering Research Center for Functional Food, Jiangnan University , Wuxi , Jiangsu Province , PR China.
authors.forename: He
authors.initials: H
authors.lastname: Qian
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002338:Carotenoids / Q000494:pharmacology*; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008297:Male; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D011467:Prostate / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: C494826:BAX protein, human; D016877:Oxidants; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; D002338:Carotenoids; C078922:torulene; D006861:Hydrogen Peroxide; C078923:torularhodin
keywords: Torulene; antioxidant effect; apoptosis; human prostate stromal cells; torularhodin