title: Identification and Characterization of Multiple Intermediate Alleles of the Key Genes Regulating Brassinosteroid Biosynthesis Pathways.
abstract: Most of the early identified brassinosteroid signaling and biosynthetic mutants are null mutants, exhibiting extremely dwarfed phenotypes and male sterility. These null mutants are usually unable to be directly transformed via a routinely usedAgrobacterium-mediated gene transformation system and therefore are less useful for genetic characterization of the brassinosteroid (BR)-related pathways. Identification of intermediate signaling mutants such asbri1-5andbri1-9has contributed drastically to the elucidation of BR signaling pathway using both genetic and biochemical approaches. However, intermediate mutants of key genes regulating BR biosynthesis have seldom been reported. Here we report identification of several intermediate BR biosynthesis mutants mainly resulted from leaky transcriptions due to the insertions of T-DNAs in the introns. These mutants are semi-dwarfed and fertile and capable to be transformed. These intermediate mutants could be useful tools for future discovery and analyses of novel components regulating BR biosynthesis and catabolism via genetic modifier screen.
authors.affiliation: College of Agronomy, Sichuan Agricultural University, Sichuan Engineering Research Center for Crop Strip Intercropping SystemChengdu, China; Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou UniversityLanzhou, China.
authors.forename: Junbo
authors.initials: J
authors.lastname: Du
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou UniversityLanzhou, China; Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of ScienceKunming, China.
authors.forename: Baolin
authors.initials: B
authors.lastname: Zhao
authors.affiliation: College of Agronomy, Sichuan Agricultural University, Sichuan Engineering Research Center for Crop Strip Intercropping System Chengdu, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Sun
authors.affiliation: College of Agronomy, Sichuan Agricultural University, Sichuan Engineering Research Center for Crop Strip Intercropping System Chengdu, China.
authors.forename: Mengyuan
authors.initials: M
authors.lastname: Sun
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University Lanzhou, China.
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University Lanzhou, China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Zhang
authors.affiliation: College of Agronomy, Sichuan Agricultural University, Sichuan Engineering Research Center for Crop Strip Intercropping System Chengdu, China.
authors.forename: Wenyu
authors.initials: W
authors.lastname: Yang
keywords: BR biosynthesis; BR catabolism; brassinolide; brassinosteroid; intermediate alleles