title: The possible protective role of pumpkin seed oil in an animal model of acid aspiration pneumonia: Light and electron microscopic study.
abstract: Aspiration pneumonitis is a common problem occurring in many clinical disorders. Pumpkin seed oil (PO) is a rich source of antioxidants. This work aimed to assess the effect of PO on the lung histopathological changes induced by acid aspiration. Forty male albino rats assigned to four groups were used. Rats of control group were instilled intratracheally with normal saline 2mL/kg. HCL group instilled with 2mL/kg of HCL 0.1N, pH 1.25. PO group received pumpkin seed oil (PO) orally (∼1375mg/kgbw/day) for 7days. HCL+PO group instilled with 2mL/kg of HCL 0.1N, pH 1.25 and received PO at the same dose of PO group. Lung tissue samples were processed for light, electron microscopic and immunohistochemical study using anti inducible NO synthase (iNOS). The lung of HCL group demonstrated thickened interalveolar septa, inflammatory cell infiltration and significant increase in the area percent of collagenous fibers and immune expression of iNOS. Ultra structurally, disrupted alveolocapillay membrane, degenerated type II pneumocytes and plentiful alveolar macrophages were evident. PO administration partially attenuated these histological and ultra structural alterations and reduced iNOS immune-expression in lung tissue. In conclusion, PO has a protective effect against HCL aspiration lung injury most probably through its antioxidant activity.
authors.affiliation: Department of Histology and Cell Biology, Faculty of Medicine, Mansoura University, Egypt. Electronic address: nesrinemoustafa@gmail.com.
authors.forename: Nesreen Moustafa
authors.initials: NM
authors.lastname: Omar
authors.affiliation: Department of Histology and Cell Biology, Faculty of Medicine, Mansoura University, Egypt.
authors.forename: Nahla Reda
authors.initials: NR
authors.lastname: Sarhan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D028464:Cucurbita / Q000737:chemistry; D019610:Cytoprotection; D008168:Lung / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D008297:Male; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D010938:Plant Oils; D011015:Pneumonia, Aspiration / Q000188:drug therapy*; D051381:Rats; D012639:Seeds / Q000737:chemistry
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D010938:Plant Oils; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat
keywords: Aspiration pneumonia; Lung; Pumpkin seed oil; Ultrastructure; iNO synthase