title: Fine-mapping qFS07.1 controlling fiber strength in upland cotton (Gossypium hirsutum L.).
abstract: KEY MESSAGE: qFS07.1 controlling fiber strength was fine-mapped to a 62.6-kb region containing four annotated genes. RT-qPCR and sequence of candidate genes identified an LRR RLK gene as the most likely candidate. Fiber strength is an important component of cotton fiber quality and is associated with other properties, such as fiber maturity, fineness, and length. Stable QTL qFS07.1, controlling fiber strength, had been identified on chromosome 7 in an upland cotton recombinant inbred line (RIL) population from a cross (CCRI35 × Yumian1) described in our previous studies. To fine-map qFS07.1, an F2population with 2484 individual plants from a cross between recombinant line RIL014 and CCRI35 was established. A total of 1518 SSR primer pairs, including 1062, designed from chromosome 1 of the Gossypium raimondii genome and 456 from chromosome 1 of the G. arboreum genome (corresponding to the QTL region) were used to fine-map qFS07.1, and qFS07.1 was mapped into a 62.6-kb genome region which contained four annotated genes on chromosome A07 of G. hirsutum. RT-qPCR and comparative analysis of candidate genes revealed a leucine-rich repeat protein kinase (LRR RLK) family protein to be a promising candidate gene for qFS07.1. Fine mapping and identification of the candidate gene for qFS07.1 will play a vital role in marker-assisted selection (MAS) and the study of mechanism of cotton fiber development.
authors.affiliation: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, 400716, Chongqing, People's Republic of China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Fang
authors.affiliation: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, 400716, Chongqing, People's Republic of China.
authors.forename: Xueying
authors.initials: X
authors.lastname: Liu
authors.affiliation: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, 400716, Chongqing, People's Republic of China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, 400716, Chongqing, People's Republic of China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Wang
authors.affiliation: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, 400716, Chongqing, People's Republic of China.
authors.forename: Dexin
authors.initials: D
authors.lastname: Liu
authors.affiliation: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, 400716, Chongqing, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, 400716, Chongqing, People's Republic of China.
authors.forename: Dajun
authors.initials: D
authors.lastname: Liu
authors.affiliation: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, 400716, Chongqing, People's Republic of China.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Teng
authors.affiliation: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, 400716, Chongqing, People's Republic of China.
authors.forename: Zhaoyun
authors.initials: Z
authors.lastname: Tan
authors.affiliation: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, 400716, Chongqing, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, 400716, Chongqing, People's Republic of China.
authors.forename: Fengjiao
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, 400716, Chongqing, People's Republic of China.
authors.forename: Maochao
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, 400716, Chongqing, People's Republic of China.
authors.forename: Xiuling
authors.initials: X
authors.lastname: Jia
authors.affiliation: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, 400716, Chongqing, People's Republic of China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, 400716, Chongqing, People's Republic of China.
authors.forename: Jinghong
authors.initials: J
authors.lastname: Yang
authors.affiliation: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, 400716, Chongqing, People's Republic of China. zhangzs@swu.edu.cn.
authors.forename: Zhengsheng
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D032561:Cotton Fiber; D005819:Genetic Markers; D018745:Genome, Plant; D003368:Gossypium / Q000235:genetics*; D000090263:Leucine-Rich Repeat Proteins; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics*; D011494:Protein Kinases / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D040641:Quantitative Trait Loci
chemical_list: D005819:Genetic Markers; D000090263:Leucine-Rich Repeat Proteins; D010940:Plant Proteins; D011506:Proteins; D011494:Protein Kinases