title: Long noncoding RNACRNDEfunctions as a competing endogenous RNA to promote metastasis and oxaliplatin resistance by sponging miR-136 in colorectal cancer.
abstract: Colorectal neoplasia differentially expressed (CRNDE) is a novel gene recognized as a long noncoding RNA (lncRNA) that is highly elevated in colorectal cancer and many other solid tumors but its functions on metastasis and oxaliplatin (OXA) resistance are unknown. In our study, we confirmed the upregulation ofCRNDEin both primary specimens from colorectal cancer patients and colorectal cancer cell lines. Knockdown ofCRNDEexpression inhibited the migration and invasion potency of colorectal cancer cells with no effect on cell apoptosis. Overexpression ofCRNDEpromoted the migration and invasion potency of colorectal cancer cells. Furthermore, we found thatCRNDEconferred chemoresistance in colorectal cancer cells. Knockdown ofCRNDEwith OXA treatment decreased cell viability and promoted DNA damage and cell apoptosis, while the overexpression ofCRNDEwith OXA treatment reduced DNA damage and cell apoptosis. Further in-depth mechanistic studies revealed thatCRNDEfunctioned as a competing endogenous RNA for miR-136, led to the de-repression of its endogenous target, E2F transcription factor 1 (E2F1). Overall, our findings demonstrate thatCRNDEfunctions as a competing endogenous RNA to promote metastasis and OXA resistance by sponging miR-136 in colorectal cancer.
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Xi'an Medical University.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Gao
authors.affiliation: Department of Pharmacy, Xi'an Central Hospital, Xi'an.
authors.forename: Xiaodi
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of Oncology, Yan'an University Affiliated Hospital, Yan'an.
authors.forename: Ting
authors.initials: T
authors.lastname: Kang
authors.affiliation: Department of Pharmacy, Hong-Hui Hospital, Xi'an Jiaotong University Medical College, Xi'an.
authors.forename: Baohong
authors.initials: B
authors.lastname: Yan
authors.affiliation: Department of Anorectal Surgery, Ankang City Central Hospital, Ankang.
authors.forename: Li
authors.initials: L
authors.lastname: Feng
authors.affiliation: Department of Pharmacy, Yan'an University Affiliated Hospital.
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of Pharmacy, Yan'an University Affiliated Hospital.
authors.forename: Liang
authors.initials: L
authors.lastname: Ai
authors.affiliation: Department of Endocrinology, Yan'an People's Hospital, Yan'an.
authors.forename: Xiaoni
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of General Surgery, Nuclear Industry 215 Hospital of Shaanxi Province, Xianyang.
authors.forename: Jie
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of General Surgery, The People's Hospital of Baoji City, Baoji, People's Republic of China.
authors.forename: Huiqi
authors.initials: H
authors.lastname: Li
keywords: CRNDE; E2F1; colorectal cancer; metastasis; miR-136; oxaliplatin resistance