title: Zenit RA evaluation, a solid-phase chemiluminescence immunoassay for detection of anti-cellular antibodies.
abstract: OBJECTIVE
  The objective was to compare Zenit RA chemiluminescent immunoassay (CLIA) from Menarini Diagnostics and ELISA from INOVA Diagnostics for the presence of specific anti-Ro/SS-A, anti-La/SS-B, anti-U1snRNP, anti-Sm, anti-Scl-70, anti-Jo-1 antibodies. Results/methodology: We studied 501 samples (178 connective autoimmune disease, 150 other autoimmune or inflammatory disease and 173 other disease or healthy). All samples were analyzed using CLIA and ELISA. The Kappa agreement was excellent for anti-SSA/Ro (0.864), good for anti-SSB/La (0.735), anti-Scl-70 (0.685) and ENA-screening (0.778), moderate for anti-RNP (0.563) and bad for anti-Sm (0.266) and anti-Jo-1 (0.243). Different combination of cut-off improved the specificity and agreement.
  
  
  CONCLUSIONS
  Zenit RA CLIA for detecting autoantibodies, provides a simple, useful and accurate tool.
authors.affiliation: UGC Clinical Biochemistry, Virgen Macarena University Hospital, Dr Fedriani 3 Avenue - 41009 Seville, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Melguizo Madrid
authors.affiliation: UGC Clinical Biochemistry, Virgen Macarena University Hospital, Dr Fedriani 3 Avenue - 41009 Seville, Spain.
authors.forename: Concepción
authors.initials: C
authors.lastname: González-Rodríguez
authors.affiliation: UGC Clinical Biochemistry, Virgen Macarena University Hospital, Dr Fedriani 3 Avenue - 41009 Seville, Spain.
authors.forename: María de Gracia
authors.initials: MG
authors.lastname: Ávila-García
authors.affiliation: UGC Clinical Biochemistry, Virgen Macarena University Hospital, Dr Fedriani 3 Avenue - 41009 Seville, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Arrobas-Velilla
authors.affiliation: UGC Clinical Biochemistry, Virgen Macarena University Hospital, Dr Fedriani 3 Avenue - 41009 Seville, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: Fernández-Riejos
mesh_terms: D000974:Antibodies, Antinuclear / Q000032:analysis*; D001327:Autoimmune Diseases / Q000175:diagnosis; D016022:Case-Control Studies; D004264:DNA Topoisomerases, Type I; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007118:Immunoassay; D016015:Logistic Models; D008163:Luminescent Measurements; D009687:Nuclear Proteins / Q000276:immunology; D017411:Ribonucleoproteins, Small Nuclear / Q000276:immunology
chemical_list: D000974:Antibodies, Antinuclear; C046750:Jo-1 antibody; D009687:Nuclear Proteins; D017411:Ribonucleoproteins, Small Nuclear; C552127:SNRNP35 protein, human; C048100:Scl 70 antigen, human; D004264:DNA Topoisomerases, Type I
keywords: CLIA; ELISA; Kappa agreement