title: Facilitated saliva secretion and reduced oral inflammation by a novel artificial saliva system in the treatment of salivary hypofunction.
abstract: Saliva substitutes and/or lubricants are commonly employed to lessen dry mouth symptoms by stimulating and/or substituting for the secretion of saliva. In this study, a novel artificial saliva containing inorganic salts, including sodium chloride and potassium chloride, and bactericidal agents, including potassium thiocyanate and lactoperoxidase, was formulated in the form of a solution (DM-sol) or gel (DM-gel). Those in vivo therapeutic efficacies were assessed in terms of saliva secretion and anti-inflammatory activity in rats and mice, respectively. Salivary secretion was promoted by mucosal application of DM-formulations in normal rats. In particular, DM-gel resulted in 2.5- and 1.9-fold greater salivary flow rates compared to normal saline and DM-sol, respectively. In an in vivo efficacy evaluation in diabetic mice with salivary hypofunction, repeated application of DM-formulations alleviated histopathological changes in the buccal mucosa in terms of atrophy and thinning of the epithelium, compared to vehicle, after 4 weeks. Moreover, the DM-sol and DM-gel were comparably effective for relieving periodontal gingivitis, reducing infiltration of inflammatory cells, and normalizing the neutrophil level in the gingival gingiva, after 4 weeks. Therefore, the novel artificial saliva is expected to facilitate salivary secretion and restore physiological conditions in the mouth of patients with salivary hypofunction.
authors.affiliation: Pharmaceutical Product Research Laboratories, Dong-A ST Research Institute, Gyeonggi.
authors.forename: Minkyung
authors.initials: M
authors.lastname: Kang
authors.affiliation: Pharmaceutical Product Research Laboratories, Dong-A ST Research Institute, Gyeonggi.
authors.forename: Hyounggeun
authors.initials: H
authors.lastname: Park
authors.affiliation: Pharmaceutical Product Research Laboratories, Dong-A ST Research Institute, Gyeonggi.
authors.forename: Joon-Ho
authors.initials: JH
authors.lastname: Jun
authors.affiliation: Pharmaceutical Product Research Laboratories, Dong-A ST Research Institute, Gyeonggi.
authors.forename: Miwon
authors.initials: M
authors.lastname: Son
authors.affiliation: Division of Pharmaceutical Sciences, College of Pharmacy, Dankook University, Cheonan, Chungnam, Korea.
authors.forename: Myung Joo
authors.initials: MJ
authors.lastname: Kang
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D005260:Female; D005891:Gingivitis / Q000150:complications / Q000188:drug therapy*; D007249:Inflammation / Q000150:complications / Q000188:drug therapy*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D017207:Rats, Sprague-Dawley; D012464:Saliva, Artificial / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D012469:Salivary Glands / Q000187:drug effects* / Q000378:metabolism*; D014987:Xerostomia / Q000150:complications / Q000188:drug therapy*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D012464:Saliva, Artificial
keywords: antimicrobial activity; carbopol gel; diabetic rats; hypothiocyanitehydrogen peroxide mixture; saliva substitute