title: DNA damage induced by Strontium-90 exposure at low concentrations in mesenchymal stromal cells: the functional consequences.
abstract: 90Sr is one of the radionuclides released after nuclear accidents that can significantly impact human health in the long term.90Sr accumulates mostly in the bones of exposed populations. Previous research has shown that exposure induces changes in bone physiology both in humans and in mice. We hypothesize that, due to its close location with bone marrow stromal cells (BMSCs),90Sr could induce functional damage to stromal cells that may explain these biological effects due to chronic exposure to90Sr. The aim of this work was to verify this hypothesis through the use of an in vitro model of MS5 stromal cell lines exposed to 1 and 10 kBq.mL-1of90Sr. Results indicated that a 30-minute exposure to90Sr induced double strand breaks in DNA, followed by DNA repair, senescence and differentiation. After 7 days of exposure, MS5 cells showed a decreased ability to proliferate, changes in cytokine expression, and changes in their ability to support hematopoietic progenitor proliferation and differentiation. These results demonstrate that chronic exposure to a low concentration of90Sr can induce functional changes in BMSCs that in turn may explain the health effects observed in following chronic90Sr exposure.
authors.affiliation: Institut de radioprotection et de sureté nucléaire (IRSN), PRP-HOM/SRBE/LRTOX, Fontenay aux roses, France.
authors.forename: S
authors.initials: S
authors.lastname: Musilli
authors.affiliation: Institut de radioprotection et de sureté nucléaire (IRSN), PRP-HOM/SRBE/LRTOX, Fontenay aux roses, France.
authors.forename: N
authors.initials: N
authors.lastname: Nicolas
authors.affiliation: UMR996 - Inflammation, Chemokines and Immunopathology, INSERM, Université Paris-Sud, Université Paris-Saclay, 92296, Châtenay-Malabry, France.
authors.forename: Z
authors.initials: Z
authors.lastname: El Ali
authors.affiliation: Institut de radioprotection et de sureté nucléaire (IRSN), PRP-HOM/SRBE/LRTOX, Fontenay aux roses, France.
authors.forename: P
authors.initials: P
authors.lastname: Orellana-Moreno
authors.affiliation: Institut de radioprotection et de sureté nucléaire (IRSN), PRP-HOM/SRBE/LRTOX, Fontenay aux roses, France.
authors.forename: C
authors.initials: C
authors.lastname: Grand
authors.affiliation: Institut de radioprotection et de sureté nucléaire (IRSN), PRP-HOM/SRBE/LRTOX, Fontenay aux roses, France.
authors.forename: K
authors.initials: K
authors.lastname: Tack
authors.affiliation: UMR996 - Inflammation, Chemokines and Immunopathology, INSERM, Université Paris-Sud, Université Paris-Saclay, 92296, Châtenay-Malabry, France.
authors.forename: S
authors.initials: S
authors.lastname: Kerdine-Römer
authors.affiliation: Institut de radioprotection et de sureté nucléaire (IRSN), PRP-HOM/SRBE/LRTOX, Fontenay aux roses, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Bertho
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D016923:Cell Death; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D016922:Cellular Senescence / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D053903:DNA Breaks, Double-Stranded / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects*; D004260:DNA Repair; D006657:Histones / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013326:Strontium Radioisotopes / Q000494:pharmacology*
chemical_list: D016207:Cytokines; D006657:Histones; D017382:Reactive Oxygen Species; D013326:Strontium Radioisotopes; C000615490:Strontium-90