title: Blood Group Determination using DNA extracted from Exfoliated Primary Teeth at Various Time Durations and Temperatures: A PCR Study.
abstract: OBJECTIVE
  To determine polymerase chain reaction (PCR)-based blood group on tooth pulp obtained from teeth stored for 1 month, 6 months, and 1 year following extraction and to evaluate the stability of deoxyribonucleic acid (DNA) in primary tooth subjected to a temperature of 200°C ± 5°C for 15 minutes.
  
  
  METHODS
  Dental pulp tissue was collected from 40 exfoliated primary teeth stored for various time durations and temperature and preserved at 4°C till DNA extraction was carried out. Deoxyribonucleic acid was extracted using silica membrane-based spin-column procedure of QIAamp DNA minikit from BioRad. Deoxyribonucleic acid was subjected to PCR amplification and monoplex allele-specific PCR primers for ABO genotyping.
  
  
  METHODS
  The data were analyzed by comparison (based on percentage).
  
  
  RESULTS
  In our study, overall, 85% samples showed a DNA yield. Cent percent results were obtained for samples studied at the end of 1 month followed by 90 and 80% for samples studied for 6 months and 1 year respectively. Heated samples showed 70% result.
  
  
  CONCLUSIONS
  Polymerase chain reaction was found to be an effective method for blood group determination for teeth stored at various time durations and temperatures. However, as the time interval increased, the number of positive results obtained decreased.
  Pai RK, Bhat SS, Salman A, Hegde S. Blood Group Determination using DNA extracted from Exfoliated Primary Teeth at Various Time Durations and Temperatures: A PCR Study. Int J Clin Pediatr Dent 2016;9(4):308-312.
authors.affiliation: Reader, Department of Pedodontics and Preventive Dentistry Yenepoya Dental College, Mangaluru, Karnataka, India.
authors.forename: Reshma K
authors.initials: RK
authors.lastname: Pai
authors.affiliation: Professor and Head, Department of Pedodontics and Preventive Dentistry Yenepoya Dental College, Mangaluru, Karnataka, India.
authors.forename: Sham S
authors.initials: SS
authors.lastname: Bhat
authors.affiliation: Senior Lecturer, Department of Pedodontics and Preventive Dentistry Yenepoya Dental College, Mangaluru, Karnataka, India.
authors.forename: Afreen
authors.initials: A
authors.lastname: Salman
authors.affiliation: Professor and Head, Department of Pedodontics and Preventive Dentistry Yenepoya Dental College, Mangaluru, Karnataka, India.
authors.forename: Sundeep
authors.initials: S
authors.lastname: Hegde
keywords: ABO; Blood grouping; Human identification; Polymerase chain reaction; Primary tooth; Pulp.