title: Time-Series Analyses of Transcriptomes and Proteomes Reveal Molecular Networks Underlying Oil Accumulation in Canola.
abstract: Understanding the regulation of lipid metabolism is vital for genetic engineering of canola (Brassica napusL.) to increase oil yield or modify oil composition. We conducted time-series analyses of transcriptomes and proteomes to uncover the molecular networks associated with oil accumulation and dynamic changes in these networks in canola. The expression levels of genes and proteins were measured at 2, 4, 6, and 8 weeks after pollination (WAP). Our results show that the biosynthesis of fatty acids is a dominant cellular process from 2 to 6 WAP, while the degradation mainly happens after 6 WAP. We found that genes in almost every node of fatty acid synthesis pathway were significantly up-regulated during oil accumulation. Moreover, significant expression changes of two genes, acetyl-CoA carboxylase and acyl-ACP desaturase, were detected on both transcriptomic and proteomic levels. We confirmed the temporal expression patterns revealed by the transcriptomic analyses using quantitative real-time PCR experiments. The gene set association analysis show that the biosynthesis of fatty acids and unsaturated fatty acids are the most significant biological processes from 2-4 WAP and 4-6 WAP, respectively, which is consistent with the results of time-series analyses. These results not only provide insight into the mechanisms underlying lipid metabolism, but also reveal novel candidate genes that are worth further investigation for their values in the genetic engineering of canola.
authors.affiliation: College of Agronomy and Biotechnology, Southwest UniversityChongqing, China; Engineering Research Center of South Upland Agriculture of Ministry of EducationChongqing, China.
authors.forename: Huafang
authors.initials: H
authors.lastname: Wan
authors.affiliation: College of Agronomy and Biotechnology, Southwest University Chongqing, China.
authors.forename: Yixin
authors.initials: Y
authors.lastname: Cui
authors.affiliation: College of Agronomy and Biotechnology, Southwest University Chongqing, China.
authors.forename: Yijuan
authors.initials: Y
authors.lastname: Ding
authors.affiliation: College of Agronomy and Biotechnology, Southwest UniversityChongqing, China; Engineering Research Center of South Upland Agriculture of Ministry of EducationChongqing, China.
authors.forename: Jiaqin
authors.initials: J
authors.lastname: Mei
authors.affiliation: College of Agronomy and Biotechnology, Southwest University Chongqing, China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Dong
authors.affiliation: College of Agronomy and Biotechnology, Southwest University Chongqing, China.
authors.forename: Wenxin
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of Agronomy and Biotechnology, Southwest University Chongqing, China.
authors.forename: Shiqi
authors.initials: S
authors.lastname: Wu
authors.affiliation: College of Agronomy and Biotechnology, Southwest UniversityChongqing, China; Engineering Research Center of South Upland Agriculture of Ministry of EducationChongqing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liang
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University Wuhan, China.
authors.forename: Chunyu
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Agronomy and Biotechnology, Southwest UniversityChongqing, China; Engineering Research Center of South Upland Agriculture of Ministry of EducationChongqing, China.
authors.forename: Jiana
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Computer Science and Technology, Southwest University Chongqing, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Xiong
authors.affiliation: College of Agronomy and Biotechnology, Southwest UniversityChongqing, China; Engineering Research Center of South Upland Agriculture of Ministry of EducationChongqing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qian
keywords: Brassica napus; lipid metabolism; molecular network; proteome; seed development; transcriptome