title: Peptidomic Analysis of Cultured Cardiomyocytes Exposed to Acute Ischemic-Hypoxia.
abstract: BACKGROUND
  Acute Myocardial Infarction (AMI) is a life-threatening cardiovascular disease involving disruption of blood flow to the heart, consequent tissue damage, and sometimes death. Peptidomics, an emerging branch of proteomics, has attracted wide attention.
  
  
  METHODS
  A comparative peptidomic profiling was used to explore changes induced by acute ischemic-hypoxia in primary cultured neonatal rat myocardial cells. Analysis of six-plex tandem mass tag (TMT) labelled peptides was performed using nanoflow liquid chromatography coupled online with an LTQ-Orbitrap Velos mass spectrometer.
  
  
  RESULTS
  A total of 220 differentially expressed peptides originating from 119 proteins were identified, of which 37 were upregulated and 183 were downregulated in cardiomyocytes exposed to hypoxia/ischemia conditions. Many of the identified peptides were derived from functional domains of proteins closely associated with cardiomyocyte structure or AMI.
  
  
  CONCLUSIONS
  Numerous peptides may be involved in process of AMI. These results pave the way for future functional studies of the identified peptides.
authors.forename: Lijie
authors.initials: L
authors.lastname: Wu
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Xing
authors.initials: X
authors.lastname: Li
authors.forename: Yumei
authors.initials: Y
authors.lastname: Chen
authors.forename: Qijun
authors.initials: Q
authors.lastname: Zhang
authors.forename: Zijie
authors.initials: Z
authors.lastname: Cheng
authors.forename: Yi
authors.initials: Y
authors.lastname: Fan
authors.forename: Lingmei
authors.initials: L
authors.lastname: Qian
authors.forename: Guixian
authors.initials: G
authors.lastname: Song
mesh_terms: D000818:Animals; D015687:Cell Hypoxia; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism; D010455:Peptides / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics; D051381:Rats; D053719:Tandem Mass Spectrometry
chemical_list: D010455:Peptides
keywords: Acute myocardial infarction; Cardiomyocytes; Hypoxia; Mass spectrometry; Peptidomics; Tandem mass tag