title: Identification of TWIST-interacting genes in prostate cancer.
abstract: Prostate cancer is one of the most common cancers in men worldwide, and the number of diagnosed patients has dramatically increased in recent years. Currently, the clinical parameters used to diagnose prostate cancer, such as Gleason score, pathological tumor staging, and prostate-specific antigen (PSA) expression level, are considered insufficient to inform recommendation to guide clinical practice. Thus, identification of a novel biomarker is necessary. TWIST is one of the well-studied targets and is correlated with cancer invasion and metastasis in several human cancers. We have investigated two largest prostate cancer patient cohorts available in GEO database and found that TWIST expression is positive correlated with Gleason score and associated with poorer survival. By using a prostate cancer cohort and a prostate cancer cell line dataset, we have identified three potential downstream targets of TWIST, PPM1A, SRP72 and TBCB. TWIST's prognostic capacity is lost when the gene is mutated. Further investigation in the prostate cancer cohort revealed that gene expression of SERPINA, STX7, PDIA2, FMP5, GP1BB, VGLL4, KCNMA1, SHMT2, SAA4 and DIDO1 influence the prognostic significance of TWIST and vice versa. Importantly, eight out of these ten genes are prognostic indicator by itself. In conclusion, our study has further confirmed that TWIST is a prognostic marker in prostate cancer, identified its potential downstream targets and genes that could possibly give additional prognostic value to predict TWIST-mediated prostate cancer progression.
authors.affiliation: Faculty of Health Sciences, University of Macau, Avenida de Universidade, Macau, 999078, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Lyu
authors.affiliation: Northern Ireland Centre for Stratified Medicine, C-TRIC Building, Altnagelvin Hospital Campus, Ulster University, Londonderry, BT47 6SB, UK.
authors.forename: Shu-Dong
authors.initials: SD
authors.lastname: Zhang
authors.affiliation: Institute of Molecular and Cellular Biology, Agency for Science, Technology and Research, Singapore, 138673, Singapore.
authors.forename: Hiu-Fung
authors.initials: HF
authors.lastname: Yuen
authors.affiliation: School of Pharmacy, Queen's University of Belfast, Belfast, BT9 7BL, UK.
authors.forename: Cian M
authors.initials: CM
authors.lastname: McCrudden
authors.affiliation: Centre for Cancer Research & Cell Biology, School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast, Belfast, BT9 7BL, UK.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wen
authors.affiliation: Department of Pathology, Queen Mary Hospital, University of Hong Kong, Hong Kong, 999077, China.
authors.forename: Kwok-Wah
authors.initials: KW
authors.lastname: Chan
authors.affiliation: Faculty of Health Sciences, University of Macau, Avenida de Universidade, Macau, 999078, China. hfkwok@umac.mo.
authors.forename: Hang Fai
authors.initials: HF
authors.lastname: Kwok
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism; D015331:Cohort Studies; D015870:Gene Expression; D006801:Humans; D008297:Male; D009361:Neoplasm Invasiveness; D011379:Prognosis; D017430:Prostate-Specific Antigen / Q000097:blood; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D012333:RNA, Messenger / Q000235:genetics; D016019:Survival Analysis; D051879:Twist-Related Protein 1 / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D051879:Twist-Related Protein 1; D017430:Prostate-Specific Antigen
keywords: TWIST; prognostic marker; prostate cancer