title: Microshell Arrays Enhanced Sensitivity in Detection of Specific Antibody for Reduced Graphene Oxide Optical Sensor.
abstract: Protein-protein interactions play an important role in the investigation of biomolecules. In this paper, we reported on the use of a reduced graphene oxide microshell (RGOM)-based optical biosensor for the determination of goat anti-rabbit IgG. The biosensor was prepared through a self-assembly of monolayers of monodisperse polystyrene microspheres, combined with a high-temperature reduction, in order to decorate the RGOM with rabbit IgG. The periodic microshells allowed a simpler functionalization and modification of RGOM with bioreceptor units, than reduced graphene oxide (RGO). With additional antibody-antigen binding, the RGOM-based biosensor achieved better real-time and label-free detection. The RGOM-based biosensor presented a more satisfactory response to goat anti-rabbit IgG than the RGO-based biosensor. This method is promising for immobilizing biomolecules on graphene surfaces and for the fabrication of biosensors with enhanced sensitivity.
authors.affiliation: The Key Laboratory of Weak Light Nonlinear Photonics, Ministry of Education, Teda Applied Physics School and School of Physics, Nankai University, Tianjin 300071, China. jws19860826@126.com.
authors.forename: Wen-Shuai
authors.initials: WS
authors.lastname: Jiang
authors.affiliation: The Key Laboratory of Weak Light Nonlinear Photonics, Ministry of Education, Teda Applied Physics School and School of Physics, Nankai University, Tianjin 300071, China. xinwei0117@126.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Xin
authors.affiliation: The Key Laboratory of Weak Light Nonlinear Photonics, Ministry of Education, Teda Applied Physics School and School of Physics, Nankai University, Tianjin 300071, China. chenshaonan@mail.nankai.edu.cn.
authors.forename: Shao-Nan
authors.initials: SN
authors.lastname: Chen
authors.affiliation: The Key Laboratory of Weak Light Nonlinear Photonics, Ministry of Education, Teda Applied Physics School and School of Physics, Nankai University, Tianjin 300071, China. lcbo710@126.com.
authors.forename: Cun-Bo
authors.initials: CB
authors.lastname: Li
authors.affiliation: The Key Laboratory of Weak Light Nonlinear Photonics, Ministry of Education, Teda Applied Physics School and School of Physics, Nankai University, Tianjin 300071, China. xiaoguang.gao@foxmail.com.
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: Gao
authors.affiliation: The Key Laboratory of Weak Light Nonlinear Photonics, Ministry of Education, Teda Applied Physics School and School of Physics, Nankai University, Tianjin 300071, China. plt@nankai.edu.cn.
authors.forename: Lei-Ting
authors.initials: LT
authors.lastname: Pan
authors.affiliation: The Key Laboratory of Weak Light Nonlinear Photonics, Ministry of Education, Teda Applied Physics School and School of Physics, Nankai University, Tianjin 300071, China. rainingstar@nankai.edu.cn.|The 2011 Project Collaborative Innovation Center for Biological Therapy, Nankai University, Tianjin 300071, China. rainingstar@nankai.edu.cn.
authors.forename: Zhi-Bo
authors.initials: ZB
authors.lastname: Liu
authors.affiliation: The Key Laboratory of Weak Light Nonlinear Photonics, Ministry of Education, Teda Applied Physics School and School of Physics, Nankai University, Tianjin 300071, China. jjtian@nankai.edu.cn.|The 2011 Project Collaborative Innovation Center for Biological Therapy, Nankai University, Tianjin 300071, China. jjtian@nankai.edu.cn.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Tian
mesh_terms: D000818:Animals; D000906:Antibodies; D015374:Biosensing Techniques; D006108:Graphite; D010087:Oxides; D011817:Rabbits
chemical_list: D000906:Antibodies; D010087:Oxides; D006108:Graphite
keywords: goat anti-rabbit IgG; label-free; microshells; optical sensor; real-time; reduced graphene oxide