title: A Method for LC-MS/MS Profiling of Coumarins in Zanthoxylum zanthoxyloides (Lam.) B. Zepernich and Timler Extracts and Essential Oils.
abstract: The metabolites from the coumarin class, present in tissues of plants belonging mainly to the Rutaceae and Apiaceae families, included compounds with high chemical diversity such as simple coumarins and furocoumarins. These health-promoting components are recognized for their valuable biological activities in herbal preparations but also for their phototoxic effects. In this work, a targeted liquid chromatography (LC) coupled with tandem mass spectrometry (MS²) was developed for the screening of 39 reference standards of coumarins and furocoumarins in essential oils and plant extracts. Chromatographic separation was accomplished on reversed phase column using water/acetonitrile as the mobile phase and detection was performed on a hybrid QqQ/linear ion trap spectrometer fitted with an atmospheric pressure chemical ionization (APCI) source operating in positive ion mode. This analytical approach was applied to investigate the coumarin compositions of fruit essential oils and methanolic extracts obtained from separated parts (fruit, leaf, stem, trunk, and root) ofZanthoxylum zanthoxyloides. Ten coumarins and six furanocoumarins were reported in this species and data analyses were used to assess the suitability of these compounds to the metabolomics-based differentiation of plant organs. The quantification criteria of the metabolites in extract samples included linearity, limit of quantification, limit of detection, and matrix effect were validated. As reported for other species of the Rutaceae family, the concentration of coumarins was drastically higher inZ. zanthoxyloidesfruits than in other plant organs.
authors.affiliation: Université de Corse, UMR CNRS 6134 SPE, Laboratoire de Chimie des Produits Naturels, Campus Grimaldi, BP 52, F-20250 Corte, France. thomastine34@gmail.com.|Laboratoire de Chimie Organique et Thérapeutique, Faculté de Médecine, Pharmacie et Odontologie, Université Cheikh Anta Diop, BP 5005 Dakar-Fann, Senegal. thomastine34@gmail.com.
authors.forename: Yoro
authors.initials: Y
authors.lastname: Tine
authors.affiliation: Université de Corse, UMR CNRS 6134 SPE, Laboratoire de Chimie des Produits Naturels, Campus Grimaldi, BP 52, F-20250 Corte, France. renucci@univ-corse.fr.
authors.forename: Franck
authors.initials: F
authors.lastname: Renucci
authors.affiliation: Université de Corse, UMR CNRS 6134 SPE, Laboratoire de Chimie des Produits Naturels, Campus Grimaldi, BP 52, F-20250 Corte, France. costa@univ-corse.fr.
authors.forename: Jean
authors.initials: J
authors.lastname: Costa
authors.affiliation: Laboratoire de Chimie Organique et Thérapeutique, Faculté de Médecine, Pharmacie et Odontologie, Université Cheikh Anta Diop, BP 5005 Dakar-Fann, Senegal. alassane.wele@ucad.edu.sn.
authors.forename: Alassane
authors.initials: A
authors.lastname: Wélé
authors.affiliation: Université de Corse, UMR CNRS 6134 SPE, Laboratoire de Chimie des Produits Naturels, Campus Grimaldi, BP 52, F-20250 Corte, France. paolini@univ-corse.fr.
authors.forename: Julien
authors.initials: J
authors.lastname: Paolini
mesh_terms: D019661:Apiaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003374:Coumarins / Q000032:analysis* / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D011564:Furocoumarins / Q000032:analysis* / Q000737:chemistry; D009822:Oils, Volatile / Q000032:analysis* / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D027821:Rutaceae / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods; D032106:Zanthoxylum / Q000737:chemistry*
chemical_list: D003374:Coumarins; D011564:Furocoumarins; D009822:Oils, Volatile; D010936:Plant Extracts
keywords: LC-APCI-MS/MS; Zanthoxylum zanthoxyloides; coumarins; essential oil