title: The Histone Deacetylase Inhibitor Suberoylanilide Hydroxamic Acid Alleviates Salinity Stress in Cassava.
abstract: Cassava (Manihot esculentaCrantz) demand has been rising because of its various applications. High salinity stress is a major environmental factor that interferes with normal plant growth and limits crop productivity. As well as genetic engineering to enhance stress tolerance, the use of small molecules is considered as an alternative methodology to modify plants with desired traits. The effectiveness of histone deacetylase (HDAC) inhibitors for increasing tolerance to salinity stress has recently been reported. Here we use the HDAC inhibitor, suberoylanilide hydroxamic acid (SAHA), to enhance tolerance to high salinity in cassava. Immunoblotting analysis reveals that SAHA treatment induces strong hyper-acetylation of histones H3 and H4 in roots, suggesting that SAHA functions as the HDAC inhibitor in cassava. Consistent with increased tolerance to salt stress under SAHA treatment, reduced Na+content and increased K+/Na+ratio were detected in SAHA-treated plants. Transcriptome analysis to discover mechanisms underlying salinity stress tolerance mediated through SAHA treatment reveals that SAHA enhances the expression of 421 genes in roots under normal condition, and 745 genes at 2 h and 268 genes at 24 h under both SAHA and NaCl treatment. The mRNA expression of genes, involved in phytohormone [abscisic acid (ABA), jasmonic acid (JA), ethylene, and gibberellin] biosynthesis pathways, is up-regulated after high salinity treatment in SAHA-pretreated roots. Among them, anallene oxide cyclase(MeAOC4) involved in a crucial step of JA biosynthesis is strongly up-regulated by SAHA treatment under salinity stress conditions, implying that JA pathway might contribute to increasing salinity tolerance by SAHA treatment. Our results suggest that epigenetic manipulation might enhance tolerance to high salinity stress in cassava.
authors.affiliation: Plant Biochemistry and Molecular Genetics Laboratory, Department of Biotechnology, Faculty of Science, Mahidol UniversityBangkok, Thailand; Plant Genomic Network Research Team, RIKEN Center for Sustainable Resource ScienceYokohama, Japan.
authors.forename: Onsaya
authors.initials: O
authors.lastname: Patanun
authors.affiliation: Plant Genomic Network Research Team, RIKEN Center for Sustainable Resource ScienceYokohama, Japan; CREST, Japan Science and Technology AgencySaitama, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Ueda
authors.affiliation: Plant Productivity Systems Research Group, RIKEN Center for Sustainable Resource Science Yokohama, Japan.
authors.forename: Misao
authors.initials: M
authors.lastname: Itouga
authors.affiliation: Plant Productivity Systems Research Group, RIKEN Center for Sustainable Resource Science Yokohama, Japan.
authors.forename: Yukari
authors.initials: Y
authors.lastname: Kato
authors.affiliation: Plant Genomic Network Research Team, RIKEN Center for Sustainable Resource Science Yokohama, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Utsumi
authors.affiliation: Plant Genomic Network Research Team, RIKEN Center for Sustainable Resource Science Yokohama, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Matsui
authors.affiliation: Plant Genomic Network Research Team, RIKEN Center for Sustainable Resource Science Yokohama, Japan.
authors.forename: Maho
authors.initials: M
authors.lastname: Tanaka
authors.affiliation: Plant Genomic Network Research Team, RIKEN Center for Sustainable Resource ScienceYokohama, Japan; CREST, Japan Science and Technology AgencySaitama, Japan.
authors.forename: Chikako
authors.initials: C
authors.lastname: Utsumi
authors.affiliation: Plant Productivity Systems Research Group, RIKEN Center for Sustainable Resource Science Yokohama, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Sakakibara
authors.affiliation: Chemical Genomics Research Group, RIKEN Center for Sustainable Resource Science Saitama, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Yoshida
authors.affiliation: Plant Biochemistry and Molecular Genetics Laboratory, Department of Biotechnology, Faculty of Science, Mahidol University Bangkok, Thailand.
authors.forename: Jarunya
authors.initials: J
authors.lastname: Narangajavana
authors.affiliation: Plant Genomic Network Research Team, RIKEN Center for Sustainable Resource ScienceYokohama, Japan; CREST, Japan Science and Technology AgencySaitama, Japan; Plant Genomic Network Science Division, Kihara Institute for Biological Research, Yokohama City UniversityYokohama, Japan.
authors.forename: Motoaki
authors.initials: M
authors.lastname: Seki
keywords: cassava; epigenetics; high salinity stress; histone modification; suberoylanilide hydroxamic acid (SAHA)