title: Revisiting the tubulin cofactors and Arl2 in the regulation of soluble αβ-tubulin pools and their effect on microtubule dynamics.
abstract: Soluble αβ-tubulin heterodimers are maintained at high concentration inside eukaryotic cells, forming pools that fundamentally drive microtubule dynamics. Five conserved tubulin cofactors and ADP ribosylation factor-like 2 regulate the biogenesis and degradation of αβ-tubulins to maintain concentrated soluble pools. Here I describe a revised model for the function of three tubulin cofactors and Arl2 as a multisubunit GTP-hydrolyzing catalytic chaperone that cycles to promote αβ-tubulin biogenesis and degradation. This model helps explain old and new data indicating these activities enhance microtubule dynamics in vivo via repair or removal of αβ-tubulins from the soluble pools.
authors.affiliation: Molecular Cellular Biology, University of California, Davis, Davis, CA 95616 jawdat@ucdavis.edu.
authors.forename: Jawdat
authors.initials: J
authors.lastname: Al-Bassam
mesh_terms: D020727:ADP-Ribosylation Factors / Q000378:metabolism / Q000502:physiology; D019281:Dimerization; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006706:Homeostasis; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism*; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism*
chemical_list: D008869:Microtubule-Associated Proteins; D018832:Molecular Chaperones; D014404:Tubulin; C410130:ARL2 protein, human; D019204:GTP-Binding Proteins; D020727:ADP-Ribosylation Factors