title: RNA interference-mediated knockdown of the hydroxyacid-oxoacid transhydrogenase gene decreases thiamethoxam resistance in adults of the whitefly Bemisia tabaci.
abstract: Bemisia tabaci has developed a high level of resistance to thiamethoxam, a second generation neonicotinoid insecticide that has been widely used to control this pest. In this study, we investigated whether hydroxyacid-oxoacid transhydrogenase (HOT) is involved in resistance to the neonicotinoid insecticide thiamethoxam in the whitefly. We cloned the full-length gene that encodes HOT in B. tabaci. Its cDNA contains a 1428-bp open reading frame encoding 475 amino acid residues. Then we evaluated the mRNA expression level of HOT in different developmental stages, and found HOT expression was significantly greater in thiamethoxam resistance adults than in thiamethoxam susceptible adults. Subsequently, seven field populations of B. tabaci adults were sampled, the expression of mRNA level of HOT significant positive correlated with thiamethoxam resistance level. At last, we used a modified gene silencing system to knock-down HOT expression in B. tabaci adults. The results showed that the HOT mRNA levels decreased by 57% and thiamethoxam resistance decreased significantly after 2 days of feeding on a diet containing HOT dsRNA. The results indicated that down-regulation of HOT expression decreases thiamethoxam resistance in B. tabaci adults.
authors.affiliation: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China.
authors.forename: Wen
authors.initials: W
authors.lastname: Xie
authors.affiliation: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China.
authors.forename: Ru-Mei
authors.initials: RM
authors.lastname: Li
authors.affiliation: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China.
authors.forename: Xiao-Mao
authors.initials: XM
authors.lastname: Zhou
authors.affiliation: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China.
authors.forename: Shao-Li
authors.initials: SL
authors.lastname: Wang
authors.affiliation: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China.
authors.forename: Qing-Jun
authors.initials: QJ
authors.lastname: Wu
authors.affiliation: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China.
authors.forename: Ni-Na
authors.initials: NN
authors.lastname: Yang
authors.affiliation: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China.
authors.forename: Ji-Xing
authors.initials: JX
authors.lastname: Xia
authors.affiliation: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China.
authors.forename: Ze-Zong
authors.initials: ZZ
authors.lastname: Yang
authors.affiliation: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China.
authors.forename: Li-Tao
authors.initials: LT
authors.lastname: Guo
authors.affiliation: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China.
authors.forename: Ya-Ting
authors.initials: YT
authors.lastname: Liu
authors.affiliation: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China.
authors.forename: You-Jun
authors.initials: YJ
authors.lastname: Zhang
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D055785:Gene Knockdown Techniques; D006430:Hemiptera / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism*; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007305:Insecticide Resistance; D007306:Insecticides / Q000633:toxicity*; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D000073943:Neonicotinoids / Q000633:toxicity*; D009574:Nitro Compounds / Q000633:toxicity*; D010078:Oxazines / Q000633:toxicity*; D034622:RNA Interference; D000077922:Thiamethoxam; D013844:Thiazoles / Q000633:toxicity*
chemical_list: D019476:Insect Proteins; D007306:Insecticides; D024101:Mitochondrial Proteins; D000073943:Neonicotinoids; D009574:Nitro Compounds; D010078:Oxazines; D013844:Thiazoles; D000077922:Thiamethoxam; D000429:Alcohol Oxidoreductases; C072878:hydroxyacid-oxoacid transhydrogenase