title: 4-Coumarate-CoA Ligase-Like GeneOsAAE3Negatively Mediates the Rice Blast Resistance, Floret Development and Lignin Biosynthesis.
abstract: Although adenosine monophosphate (AMP) binding domain is widely distributed in multiple plant species, detailed molecular functions of AMP binding proteins (AMPBPs) in plant development and plant-pathogen interaction remain unclear. In the present study, we identified an AMPBPOsAAE3from a previous analysis of early responsive genes in rice duringMagnaporthe oryzaeinfection.OsAAE3is a homolog of ArabidopsisAAE3in rice, which encodes a 4-coumarate-Co-A ligase (4CL) like protein. A phylogenetic analysis showed thatOsAAE3was most likely4CL-like 10in an independent group.OsAAE3was localized to cytoplasm, and it could be expressed in various tissues. Histochemical staining of transgenic plants carryingOsAAE3promoter-driven GUS (β-glucuronidase) reporter gene suggested thatOsAAE3was expressed in all tissues of rice. Furthermore,OsAAE3-OXplants showed increased susceptibility toM. Oryzae, and this finding was attributable to decreased expression of pathogen-related 1a (PR1) and low level of peroxidase (POD) activity. Moreover,OsAAE3over-expression resulted in increased content of H2O2, leading to programmed cell-death induced by reactive oxygen species (ROS). In addition,OsAAE3over-expression repressed the floret development, exhibiting dramatically twisted glume and decreased fertility rate of anther. Meanwhile, the expressions of lignin biosynthesis genes were significantly decreased inOsAAE3-OXplants, thereby leading to reduced lignin content. Taken together,OsAAE3functioned as a negative regulator in rice blast resistance, floret development, and lignin biosynthesis. Our findings further expanded the knowledge in functions of AMBPs in plant floret development and the regulation of rice-fungus interaction.
authors.affiliation: National Engineering Research Center of Plant Space Breeding, South China Agricultural University Guangzhou, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Rice Breeding, Jiamusi Rice Research Institute of Heilongjiang Academy of Agricultural Sciences Jiamusi, China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Guo
authors.affiliation: National Engineering Research Center of Plant Space Breeding, South China Agricultural University Guangzhou, China.
authors.forename: Fengwei
authors.initials: F
authors.lastname: Gu
authors.affiliation: Department of Plant Breeding, College of Agricultural, South China Agricultural University Guangzhou, China.
authors.forename: Shanwen
authors.initials: S
authors.lastname: Ke
authors.affiliation: Plant Protection Research Institute Guangdong Academy of Agricultural Sciences/Guangdong Provincial key Laboratory of High Technology for Plant Protection Guangzhou, China.
authors.forename: Dayuan
authors.initials: D
authors.lastname: Sun
authors.affiliation: National Engineering Research Center of Plant Space Breeding, South China Agricultural University Guangzhou, China.
authors.forename: Shuangyu
authors.initials: S
authors.lastname: Dong
authors.affiliation: National Engineering Research Center of Plant Space Breeding, South China Agricultural University Guangzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: National Engineering Research Center of Plant Space Breeding, South China Agricultural University Guangzhou, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Huang
authors.affiliation: National Engineering Research Center of Plant Space Breeding, South China Agricultural University Guangzhou, China.
authors.forename: Wuming
authors.initials: W
authors.lastname: Xiao
authors.affiliation: National Engineering Research Center of Plant Space Breeding, South China Agricultural University Guangzhou, China.
authors.forename: Guili
authors.initials: G
authors.lastname: Yang
authors.affiliation: National Engineering Research Center of Plant Space Breeding, South China Agricultural University Guangzhou, China.
authors.forename: Yongzhu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: National Engineering Research Center of Plant Space Breeding, South China Agricultural University Guangzhou, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Guo
authors.affiliation: National Engineering Research Center of Plant Space Breeding, South China Agricultural University Guangzhou, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: National Engineering Research Center of Plant Space Breeding, South China Agricultural University Guangzhou, China.
authors.forename: Jiafeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: National Engineering Research Center of Plant Space Breeding, South China Agricultural University Guangzhou, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Chen
keywords: 4CL-like; AMPBP; Lignin; ROS; Rice blast