title: Plasma Triglyceride Levels May Be Modulated by Gene Expression of IQCJ, NXPH1, PHF17 and MYB in Humans.
abstract: A genome-wide association study (GWAS) by our group identified loci associated with the plasma triglyceride (TG) response to ω-3 fatty acid (FA) supplementation inIQCJ,NXPH1,PHF17andMYB. Our aim is to investigate potential mechanisms underlying the associations between single nucleotide polymorphisms (SNPs) in the four genes and TG levels following ω-3 FA supplementation. 208 subjects received 3 g/day of ω-3 FA (1.9-2.2 g of EPA and 1.1 g of docosahexaenoic acid (DHA)) for six weeks. Plasma TG were measured before and after the intervention. 67 SNPs were selected to increase the density of markers near GWAS hits. Genome-wide expression and methylation analyses were conducted on respectively 30 and 35 participants' blood sample together with in silico analyses. Two SNPs ofIQCJshowed different affinities to splice sites depending on alleles. Expression levels were influenced by genotype for one SNP inNXPH1and one inMYB. Associations between 12 tagged SNPs ofIQCJ, 26 ofNXPH1, seven ofPHF17and four ofMYBand gene-specific CpG site methylation levels were found. The response of plasma TG to ω-3 FA supplementation may be modulated by the effect of DNA methylation on expression levels of genes revealed by GWAS.
authors.affiliation: Institute of Nutrition and Functional Foods (INAF), Laval University, 2440 Hochelaga Blvd, Quebec, QC G1V 0A6, Canada. bastien.vallee-marcotte.1@ulaval.ca.
authors.forename: Bastien
authors.initials: B
authors.lastname: Vallée Marcotte
authors.affiliation: Institute of Nutrition and Functional Foods (INAF), Laval University, 2440 Hochelaga Blvd, Quebec, QC G1V 0A6, Canada. frederic.guenard@fsaa.ulaval.ca.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Guénard
authors.affiliation: Institute of Nutrition and Functional Foods (INAF), Laval University, 2440 Hochelaga Blvd, Quebec, QC G1V 0A6, Canada. hubert.cormier.1@ulaval.ca.
authors.forename: Hubert
authors.initials: H
authors.lastname: Cormier
authors.affiliation: Institute of Nutrition and Functional Foods (INAF), Laval University, 2440 Hochelaga Blvd, Quebec, QC G1V 0A6, Canada. simone.lemieux@fsaa.ulaval.ca.
authors.forename: Simone
authors.initials: S
authors.lastname: Lemieux
authors.affiliation: Institute of Nutrition and Functional Foods (INAF), Laval University, 2440 Hochelaga Blvd, Quebec, QC G1V 0A6, Canada. patrick.couture@crchudequebec.ulaval.ca.|CHU de Québec Research Center-Endocrinology and Nephrology, 2705 Laurier Blvd, Quebec, QC G1V 4G2, Canada. patrick.couture@crchudequebec.ulaval.ca.
authors.forename: Patrick
authors.initials: P
authors.lastname: Couture
authors.affiliation: CHU de Québec Research Center-Endocrinology and Nephrology, 2705 Laurier Blvd, Quebec, QC G1V 4G2, Canada. iwona.rudkowska@crchudequebec.ulaval.ca.
authors.forename: Iwona
authors.initials: I
authors.lastname: Rudkowska
authors.affiliation: Institute of Nutrition and Functional Foods (INAF), Laval University, 2440 Hochelaga Blvd, Quebec, QC G1V 0A6, Canada. marie-claude.vohl@fsaa.ulaval.ca.|CHU de Québec Research Center-Endocrinology and Nephrology, 2705 Laurier Blvd, Quebec, QC G1V 4G2, Canada. marie-claude.vohl@fsaa.ulaval.ca.
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Vohl
mesh_terms: D000328:Adult; D019175:DNA Methylation / Q000235:genetics; D005786:Gene Expression Regulation; D055106:Genome-Wide Association Study; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D009479:Neuropeptides / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014280:Triglycerides / Q000097:blood*; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D006023:Glycoproteins; D018398:Homeodomain Proteins; C469360:JADE1 protein, human; D009479:Neuropeptides; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D014280:Triglycerides; D025521:Tumor Suppressor Proteins; C112162:neurexophilin
keywords: epigenetics; gene-diet interactions; genome-wide association study; nutrigenetics; plasma lipid levels; ω-3 fatty acids