title: Evaluation of a 2-aminoimidazole variant as adjuvant treatment for dermal bacterial infections.
abstract: 2-Aminoimidazole (2-AI)-based compounds have been shown to efficiently disrupt biofilm formation, disperse existing biofilms, and resensitize numerous multidrug-resistant bacteria to antibiotics. UsingPseudomonas aeruginosaandStaphylococcus aureus, we provide initial pharmacological studies regarding the application of a 2-AI as a topical adjuvant for persistent dermal infections. In vitro assays indicated that the 2-AIH10is nonbactericidal, resensitizes bacteria to antibiotics, does not harm the integument, and promotes wound healing. Furthermore, in vivo application ofH10on swine skin caused no gross abnormalities or immune reactions. Taken together, these results indicate thatH10represents a promising lead dermal adjuvant compound.
authors.affiliation: Department of Molecular and Structural Biochemistry.
authors.forename: G Logan
authors.initials: GL
authors.lastname: Draughn
authors.affiliation: Department of Molecular and Structural Biochemistry.
authors.forename: C Leigh
authors.initials: CL
authors.lastname: Allen
authors.affiliation: Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Routh
authors.affiliation: Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
authors.forename: Maria R
authors.initials: MR
authors.lastname: Stone
authors.affiliation: Center for Biofilm Engineering, Montana State University, Bozeman, MT, USA.
authors.forename: Kelly R
authors.initials: KR
authors.lastname: Kirker
authors.affiliation: Center for Biofilm Engineering, Montana State University, Bozeman, MT, USA.
authors.forename: Laura
authors.initials: L
authors.lastname: Boegli
authors.affiliation: Department of Molecular and Structural Biochemistry.
authors.forename: Ryan M
authors.initials: RM
authors.lastname: Schuchman
authors.affiliation: Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
authors.forename: Keith E
authors.initials: KE
authors.lastname: Linder
authors.affiliation: Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
authors.forename: Ronald E
authors.initials: RE
authors.lastname: Baynes
authors.affiliation: Center for Biofilm Engineering, Montana State University, Bozeman, MT, USA.
authors.forename: Garth
authors.initials: G
authors.lastname: James
authors.affiliation: Department of Chemistry, North Carolina State University, Raleigh, NC, USA.
authors.forename: Christian
authors.initials: C
authors.lastname: Melander
authors.affiliation: Agile Sciences Inc., Raleigh, NC, USA.
authors.forename: Angela
authors.initials: A
authors.lastname: Pollard
authors.affiliation: Department of Molecular and Structural Biochemistry.
authors.forename: John
authors.initials: J
authors.lastname: Cavanagh
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D018441:Biofilms / Q000187:drug effects; D017024:Chemotherapy, Adjuvant; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007093:Imidazoles / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D017192:Skin Diseases, Bacterial / Q000188:drug therapy*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D007093:Imidazoles; C014973:2-aminoimidazole
keywords: ESKAPE pathogens; antimicrobial activity; drip-flow reactor; oroidin derivative; skin irritation; synergism; transdermal absorption