title: Activity of fibroblast growth factor receptor inhibitors TKI258, ponatinib and AZD4547 against TPR‑FGFR1 fusion.
abstract: 8p11 myeloproliferative syndrome (EMS) is a rare disease characterized by the constitutive activation of fibroblast growth factor receptor 1 (FGFR1). To date, four cases of EMS with the chromosomal translocation, t(1;8)(q25;p11.2), have been reported. In the present study, TPR‑FGFR1‑expressing Baf3 cells were established and confirmed by polymerase chain reaction. To identify the most promising drug for EMS, the activities and associated mechanism of three tyrosine kinase inhibitors (TKIs), TKI258, ponatinib and AZD4547, against TPR‑FGFR1 were tested by MTT assay, flow cytometry and western blot. The data demonstrated that TPR‑FGFR1 was localized in the cytoplasm, and was able to transform interleukin-3-dependent hematopoietic Baf3 cells into growth factor‑independent cells. All of the three TKIs markedly inhibited the proliferation of TPR‑FGFR1‑expressing Baf3 cells, and the activation of FGFR1 and the downstream signaling molecules, extracellular signal‑regulated kinase 1/2, phospholipiase Cγ and signal transducer and activator of transcription 5. AZD4547 was the most efficient drug, and TKI258 was the least. By contrast, no significant difference was found among the three drugs on their effect on cell apoptosis. Taken together, the data obtained in the present study suggested that AZD4547 had increased potency, compared with TKI258 and ponatinib, for the treatment of EMS.
authors.affiliation: Department of Hematology, Jinling Hospital, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Xu-Hua
authors.initials: XH
authors.lastname: Qiu
authors.affiliation: Department of Hematology, Jinling Hospital, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Hematology, Jinling Hospital, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Hong-Qin
authors.initials: HQ
authors.lastname: Cao
authors.affiliation: Department of Hematology, Jinling Hospital, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Shao
authors.affiliation: Department of Hematology, Jinling Hospital, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Jian-Gang
authors.initials: JG
authors.lastname: Mei
authors.affiliation: Department of Hematology, Jinling Hospital, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Han-Qing
authors.initials: HQ
authors.lastname: Li
authors.affiliation: Department of Hematology, Jinling Hospital, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Zhai
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D001549:Benzamides / Q000494:pharmacology*; D001562:Benzimidazoles / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D015870:Gene Expression; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology*; D009196:Myeloproliferative Disorders / Q000188:drug therapy / Q000235:genetics; D028861:Nuclear Pore Complex Proteins / Q000235:genetics / Q000378:metabolism; D015514:Oncogene Proteins, Fusion / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D010879:Piperazines / Q000494:pharmacology*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D021381:Protein Transport; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D011720:Pyrazoles / Q000494:pharmacology*; D011724:Pyridazines / Q000494:pharmacology*; D015363:Quinolones / Q000494:pharmacology*; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014178:Translocation, Genetic
chemical_list: C500007:4-amino-5-fluoro-3-(5-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl)quinolin-2(1H)-one; C572463:AZD4547; D001549:Benzamides; D001562:Benzimidazoles; D007093:Imidazoles; D028861:Nuclear Pore Complex Proteins; D015514:Oncogene Proteins, Fusion; D010879:Piperazines; D047428:Protein Kinase Inhibitors; D011518:Proto-Oncogene Proteins; D011720:Pyrazoles; D011724:Pyridazines; D015363:Quinolones; C073597:TPR protein, human; C545373:ponatinib; D051496:Receptor, Fibroblast Growth Factor, Type 1