title: Evaluation of Metallo-β-Lactamase-Production and Carriage ofbla-VIMGenes inPseudomonas aeruginosaIsolated from Burn Wound Infections in Isfahan.
abstract: BACKGROUND
  Metallo-β-lactamase-production among Gram-negative bacteria, includingPseudomonas aeruginosa, has become a challenge for treatment of infections due to these resistant bacteria.
  
  
  OBJECTIVE
  The aim of the current study was to evaluate the metallo-β-lactamase-production and carriage ofbla-VIMgenes among carbapenem-resistantP. aeruginosaisolated from burn wound infections.
  
  
  METHODS
  A cross-sectional study was conducted from September 2014 to July 2015. One hundred and fiftyP. aeruginosaisolates were recovered from 600 patients with burn wound infections treated at Imam-Musa-Kazem Hospital in Isfahan city, Iran. Carbapenem-resistantP. aeruginosaisolates were screened by disk diffusion using CLSI guidelines. Metallo-β-lactamase-producingP. aeruginosaisolates were identified using an imipenem-EDTA double disk synergy test (EDTA-IMP DDST). For detection of MBL genes includingbla-VIM-1andbla-VIM-2, polymerase chain reaction (PCR) methods and sequencing were used.
  
  
  RESULTS
  Among the 150P. aeruginosaisolates, 144 (96%) were resistant to imipenem by the disk diffusion method, all of which were identified as metallo-β-lactamase-producingP. aeruginosaisolates by EDTA-IMP DDST. Twenty-seven (18%) and 8 (5.5%) MBL-producingP. aeruginosaisolates harboredbla-VIM-1andbla-VIM-2genes, respectively.
  
  
  CONCLUSIONS
  Our findings showed a high occurrence of metallo-β-lactamase production amongP. aeruginosaisolates in burn patient infections in our region. Also, there areP. aeruginosaisolates carrying thebla-VIM-1andbla-VIM-2genes in Isfahan province.
authors.affiliation: Trauma Research Center, Kashan University of Medical Sciences, Kashan, IR Iran.
authors.forename: Mahmood
authors.initials: M
authors.lastname: Saffari
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan, IR Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Firoozeh
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan, IR Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Pourbabaee
authors.affiliation: Department of Parasitology and Mycology, School of Medicine, Alborz University of Medical Sciences, Karaj, IR Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Zibaei
keywords: Burn Patients; Metallo-β-Lactamase; Pseudomonas aeruginosa; blaVIM-1