title: Characterization of T Cells Specific for CFP-10 and ESAT-6 in Mycobacterium tuberculosis-Infected Mauritian Cynomolgus Macaques.
abstract: Nonhuman primates can be used to study host immune responses toMycobacterium tuberculosisMauritian cynomolgus macaques (MCMs) are a unique group of animals that have limited major histocompatibility complex (MHC) genetic diversity, such that MHC-identical animals can be infected withM. tuberculosisTwo MCMs homozygous for the relatively common M1 MHC haplotype were bronchoscopically infected with 41 CFU of theM. tuberculosisErdman strain. Four other MCMs, which had at least one copy of the M1 MHC haplotype, were infected with a lower dose of 3 CFUM. tuberculosisAll animals mounted similar T-cell responses to CFP-10 and ESAT-6. Two epitopes in CFP-10 were characterized, and the MHC class II alleles restricting them were determined. A third epitope in CFP-10 was identified but exhibited promiscuous restriction. The CFP-10 and ESAT-6 antigenic regions targeted by T cells in MCMs were comparable to those seen in cases of humanM. tuberculosisinfection. Our data lay the foundation for generating tetrameric molecules to study epitope-specific CD4 T cells inM. tuberculosis-infected MCMs, which may guide future testing of tuberculosis vaccines in nonhuman primates.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Amy
authors.initials: A
authors.lastname: Ellis
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Alexis
authors.initials: A
authors.lastname: Balgeman
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Rodgers
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
authors.forename: Cassaundra
authors.initials: C
authors.lastname: Updike
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
authors.forename: Jaime
authors.initials: J
authors.lastname: Tomko
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
authors.forename: Pauline
authors.initials: P
authors.lastname: Maiello
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
authors.forename: Charles A
authors.initials: CA
authors.lastname: Scanga
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA slfeinberg@wisc.edu.|Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Shelby L
authors.initials: SL
authors.lastname: O'Connor
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry / Q000276:immunology*; D006649:Histocompatibility Antigens / Q000235:genetics / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D008252:Macaca fascicularis; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D000072078:Positron Emission Tomography Computed Tomography; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D014057:Tomography, X-Ray Computed; D014376:Tuberculosis / Q000175:diagnosis / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C117940:CFP-10 protein, Mycobacterium tuberculosis; C093276:ESAT-6 protein, Mycobacterium tuberculosis; D018984:Epitopes, T-Lymphocyte; D006649:Histocompatibility Antigens; D000949:Histocompatibility Antigens Class II; D010455:Peptides; D007371:Interferon-gamma
keywords: CFP-10; ESAT-6; Mauritian cynomolgus macaque; T cells; T-cell immunity; adaptive immunity; epitope; macaque; major histocompatibility complex; tuberculosis