title: The monoheme cytochrome c subunit of Alternative Complex III is a direct electron donor to caa3 oxygen reductase in Rhodothermus marinus.
abstract: Alternative Complex III (ACIII) is an example of the robustness and flexibility of prokaryotic respiratory chains. It performs quinol:cytochrome c oxidoreductase activity, being functionally equivalent to the bc1 complex but structurally unrelated. In this work we further explored ACIII investigating the role of its monoheme cytochrome c subunit (ActE). We expressed and characterized the individually isolated ActE, which allowed us to suggest that ActE is a lipoprotein and to show its function as a direct electron donor to the caa3 oxygen reductase.
authors.affiliation: .
authors.forename: Patrícia N
authors.initials: PN
authors.lastname: Refojo
authors.affiliation: .
authors.forename: Filipa
authors.initials: F
authors.lastname: Calisto
authors.affiliation: .
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Ribeiro
authors.affiliation: .
authors.forename: Miguel
authors.initials: M
authors.lastname: Teixeira
authors.affiliation: .
authors.forename: Manuela M
authors.initials: MM
authors.lastname: Pereira
mesh_terms: D003574:Cytochrome c Group / Q000378:metabolism*; D045264:Cytochromes a / Q000378:metabolism*; D045266:Cytochromes a3 / Q000378:metabolism*; D004579:Electron Transport; D014450:Electron Transport Complex III / Q000737:chemistry* / Q000378:metabolism*; D050356:Lipid Metabolism; D008958:Models, Molecular; D010088:Oxidoreductases / Q000378:metabolism*; D011487:Protein Conformation; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism*; D045245:Rhodothermus / Q000201:enzymology*
chemical_list: D003574:Cytochrome c Group; D045266:Cytochromes a3; D021122:Protein Subunits; C435384:cytochrome caa(3); D045264:Cytochromes a; D010088:Oxidoreductases; D014450:Electron Transport Complex III
keywords: bc1 complex; cytochrome c oxidase; lipoprotein; quinone; respiratory chain