title: Au@Ag SERRS tags coupled to a lateral flow immunoassay for the sensitive detection of pneumolysin.
abstract: Establishing a definitive diagnosis of pneumonia using conventional tests is difficult and expensive. Lateral flow immunoassays (LFIAs) are an advantageous point of care (POC) test option, but they have some limitations in terms of detection and quantification. In this work we have developed a lateral flow immunoassay for the ultrasensitive detection of penumolysin employing plasmonic Surface-Enhanced Resonance Raman Scattering (SERRS) tag as labelled probe. The combination of Au@Ag core-shell nanoparticles as plasmonic platform and Rhodamine B Isothiocyanate as Raman reporter has allowed us to fabricate a SERRS tag with high efficiency and reliability. The limit of detection of the SERRS-based LFIA was 1 pg mL-1. This could be a strong foundation for a pneumonia diagnosis test based on pneumolysin detection.
authors.affiliation: Departamento de Química Física y Analítica, Universidad de Oviedo, 33006 Oviedo, Spain. cblanco@uniovi.es.
authors.forename: Lucía
authors.initials: L
authors.lastname: Blanco-Covián
authors.affiliation: Departamento de Química Física and CINBIO, Universidade de Vigo, 36310 Vigo, Spain. juste@uvigo.es.
authors.forename: Verónica
authors.initials: V
authors.lastname: Montes-García
authors.affiliation: Centre for Molecular Nanometrology, Department of Pure and Applied Chemistry, WestCHEM, University of Strathclyde, Glasgow G1 1RD, UK.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Girard
authors.affiliation: Departamento de Química Física y Analítica, Universidad de Oviedo, 33006 Oviedo, Spain. cblanco@uniovi.es.
authors.forename: M Teresa
authors.initials: MT
authors.lastname: Fernández-Abedul
authors.affiliation: Departamento de Química Física and CINBIO, Universidade de Vigo, 36310 Vigo, Spain. juste@uvigo.es.
authors.forename: Jorge
authors.initials: J
authors.lastname: Pérez-Juste
authors.affiliation: Departamento de Química Física and CINBIO, Universidade de Vigo, 36310 Vigo, Spain. juste@uvigo.es.
authors.forename: Isabel
authors.initials: I
authors.lastname: Pastoriza-Santos
authors.affiliation: Centre for Molecular Nanometrology, Department of Pure and Applied Chemistry, WestCHEM, University of Strathclyde, Glasgow G1 1RD, UK.
authors.forename: Karen
authors.initials: K
authors.lastname: Faulds
authors.affiliation: Centre for Molecular Nanometrology, Department of Pure and Applied Chemistry, WestCHEM, University of Strathclyde, Glasgow G1 1RD, UK.
authors.forename: Duncan
authors.initials: D
authors.lastname: Graham
authors.affiliation: Departamento de Química Física y Analítica, Universidad de Oviedo, 33006 Oviedo, Spain. cblanco@uniovi.es.
authors.forename: M Carmen
authors.identifier: http://orcid.org/0000-0002-9776-9013
authors.initials: MC
authors.lastname: Blanco-López