title: Cellular and transcriptomic response to treatment with the probiotic candidate Vibrio lentus in gnotobiotic sea bass (Dicentrarchus labrax) larvae.
abstract: The present study aimed at evaluating the cellular and transcriptomic responses induced by the probiotic candidate Vibrio lentus with gnotobiotic European sea bass (Dicentrarchus labrax, Linnaeus 1785) larvae. For this, a histomorphological analysis was performed using the terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling (TUNEL) and the anti-proliferating cell nuclear antigen (PCNA) assay. In addition, a global transcriptomic approach was adopted to study the whole body mRNA changes upon administration of V. lentus by microarrays with the custom Agilent sea bass oligonucleotide-microarray v2.0 (4 × 44 K). Following V. lentus administration, the apoptotic and cell proliferative indexes did not show significant differences between treatments for hindgut nor for midgut. However, V. lentus treatment did significantly modify the gene expression related not only to cell proliferation and cell death, but also to cell adhesion, reactive oxygen species metabolism, iron transport, and immune response. Our data represent the first global analysis of the effects of the probiotic candidate V. lentus on the gene expression profile in gnotobiotic European sea bass, and as such, provides a first delineation of the mechanisms by which this agent interacts with its host and exerts its beneficial effects.
authors.affiliation: Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: Marlien
authors.initials: M
authors.lastname: Schaeck
authors.affiliation: Department of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Barcelona, Spain.
authors.forename: Felipe E
authors.initials: FE
authors.lastname: Reyes-López
authors.affiliation: Department of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Barcelona, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Vallejos-Vidal
authors.affiliation: Department of Virology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: Jolien
authors.initials: J
authors.lastname: Van Cleemput
authors.affiliation: Department of Comparative Physiology and Biometrics, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: Luc
authors.initials: L
authors.lastname: Duchateau
authors.affiliation: Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: Wim
authors.initials: W
authors.lastname: Van den Broeck
authors.affiliation: Department of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Barcelona, Spain.
authors.forename: Lluis
authors.initials: L
authors.lastname: Tort
authors.affiliation: Department of Pathology, Bacteriology and Avian diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium. Electronic address: Annemie.decostere@ugent.be.
authors.forename: Annemie
authors.initials: A
authors.lastname: Decostere
mesh_terms: D000818:Animals; D017209:Apoptosis; D001492:Bass / Q000502:physiology*; D049109:Cell Proliferation; D020895:Enterocytes / Q000502:physiology; D005856:Germ-Free Life; D007113:Immunity, Innate / Q000502:physiology*; D019936:Probiotics; D011897:Random Allocation; D014158:Transcription, Genetic / Q000502:physiology*; D014733:Vibrio / Q000502:physiology*
keywords: Histology; Immune response; Microarrays; Probiotics; Sea bass; Vibrio