title: Clinical efficacy of emollients in atopic dermatitis patients - relationship with the skin microbiota modification.
abstract: BACKGROUND
  We speculated that an emollient supplemented with a biomass of nonpathogenic bacteria such asVitreoscilla filiformis (Vf), grown in a medium containing thermal spring water (LRP-TSW); (LRP-Vitreoscilla filiformisbiomass [LRP-VFB]), could have a beneficial effect for patients with atopic dermatitis (AD).
  
  
  METHODS
  This double-blind, randomized, comparative study was conducted with 60 patients with moderate AD. Before starting the study, participants were pretreated for 15 days with drug therapy to improve their SCORing Atopic Dermatitis (SCORAD) by at least 25%. On Day 1, the eligible patients were randomized to either the emollient containing LRP-VFB associated with mannose (Product A) or another emollient (product B) and were treated twice daily for 1 month. Recurrence of flare-ups and microbial communities were characterized from swabs taken at Day 1 and Day 28, under axenic conditions, from affected (AF) and proximal unaffected (UAF) skin areas.
  
  
  RESULTS
  At Day 1, the average SCORAD of each group and the microbial communities of AF and UAF areas for each participant were similar. One month after the end of the therapeutic treatment (Day 28), the average evolution of SCORAD at Day 28 compared to Day 1 of patients treated with product A was significantly lower than that of the patients treated with product B. A significantly increased level ofXanthomonasgenus was noticed in the group treated with product A (versus product B). On the other hand, the level ofStaphylococcusgenus increased between Day 1 and Day 28 in the group treated with product B, but not in the group treated with product A. Interestingly, these differences were more pronounced for patients in relapse, and the associated SCORAD worsening was less in the group treated with product A versus the group treated with product B.
  
  
  CONCLUSIONS
  This study demonstrated that a specific emollient containing a biomass of non-pathogenic bacteriaVfgrown in a medium containing TSW and associated with a selected carbon source is able to normalize skin microbiota and significantly reduce the number and severity of flare-ups compared with another emollient.
authors.affiliation: La Roche-Posay Dermatological Laboratories, Asnières, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Seité
authors.affiliation: DOST, Private Clinic of Dermatovenereology, Svidnik, Slovakia.
authors.forename: Hana
authors.initials: H
authors.lastname: Zelenkova
authors.affiliation: L'Oréal Research and Innovation, Tours, France.
authors.forename: Richard
authors.initials: R
authors.lastname: Martin
keywords: Vitreoscilla filiformis; Xanthomonas genus; atopic dermatitis; emollient; skin microbiota; thermal spring water