title: Isolation of Immune Cells for Adoptive Transfer.
abstract: Adoptive transfer of T lymphocytes is a useful technique to characterize the role of the immune system in hypertension and vascular disease. Here we describe as an example the isolation of splenic T regulatory cells from donor mice processed to obtain a single cell suspension, followed by negative and positive selection to obtain CD4+T cells and CD4+CD25+Treg cells, respectively. Treg cells can be subsequently transferred to recipient animals.
authors.affiliation: Lady Davis Institute for Medical Research, Jewish General Hospital, McGill University, Montreal, QC, Canada.
authors.forename: Tlili
authors.initials: T
authors.lastname: Barhoumi
authors.affiliation: Lady Davis Institute for Medical Research, Jewish General Hospital, McGill University, Montreal, QC, Canada.
authors.forename: Pierre
authors.initials: P
authors.lastname: Paradis
authors.affiliation: Lady Davis Institute for Medical Research, Jewish General Hospital, McGill University, Montreal, QC, Canada.|Department of Oncology, Jewish General Hospital, McGill University, Montreal, QC, Canada.
authors.forename: Koren K
authors.initials: KK
authors.lastname: Mann
authors.affiliation: Lady Davis Institute for Medical Research and Department of Medicine, Jewish General Hospital, McGill University, Montreal, QC, Canada. ernesto.schiffrin@mcgill.ca.
authors.forename: Ernesto L
authors.initials: EL
authors.lastname: Schiffrin
mesh_terms: D019264:Adoptive Transfer / Q000379:methods*; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000378:metabolism; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D007275:Injections, Intravenous; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D051379:Mice; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism*
chemical_list: C508017:IL2RA protein, human; D053645:Interleukin-2 Receptor alpha Subunit
keywords: Adoptive transfer; CD4+CD25+ Treg cells; Flow cytometry; Immunofluorescence; Intravenous injection; Magnetic beads; T Cells