title: "Meniscal" scar as a landmark for the joint line in revision total knee replacement.
abstract: OBJECTIVE
  To determine whether tissue identified at the joint line was actually remnant "meniscal" scar tissue or not.
  
  
  METHODS
  Nine patients undergoing revision knee surgery following informed consent had meniscal scar tissue sent to the histology department for analyses. All revisions were performed where joint line had been raised or lowered at earlier surgery. Although preoperative radiographic evaluations suggested that the joint line had been altered, intraoperatively there was scar tissue at the level of the recreated joint line. This scar tissue has traditionally been described as meniscal scar, and to identify the origins of this tissue, samples were sent for histological analyses. The tissue samples were stored in formalin, and embedded and sectioned before undergoing histochemical staining. All samples underwent macroscopic and microscopic examination by a histopathologist who was blind to the study aims. The specific features that were examined included tissue organisation, surface and central composition, cellular distribution including histiocytes, nuclear ratio and vasculature. Atypical and malignant features, inflammation and degeneration were specifically looked for. A statistical review of the study was performed by a biomedical statistician.
  
  
  RESULTS
  The histological findings for the nine patients showing the macroscopic and microscopic findings, and the conclusion are outlined in a Table. The histological analyses were reviewed to determine whether the tissue samples were likely to be meniscal scar tissue. The response was yes (2, 22%), no (6, 67%) and maybe (1, 11%) based on the conclusions. The results were "yes" when on macroscopy, firm cream tissue was identified. In these two "yes" samples, microscopic analyses showed organised fibrous tissue with focal degenerative areas with laminated pattern associated with histiocytes peripherally but no inflammation. The "no" samples were assessed macroscopically and microscopically and were deemed to have appearances representing fibrous synovial tissue and features in keeping with degenerate scar tissue or connective tissue. One sample was indeterminate and microscopically contained fibro-collagenous tissue with synovial hyperplasia. It also contained some degenerate hyalinised tissue that may represent cartilage, but the appearances were not specific.
  
  
  CONCLUSIONS
  Based on our pilot study, we recommend reliance on a number of markers to identify the joint line as outlined above, and to exercise caution in using the "meniscal" scar.
authors.affiliation: Wasim Sardar Khan, Jagmeet Bhamra, Rhodri Williams, Rhidian Morgan-Jones, Cardiff and Vale Orthopaedic Centre, Llandough University Hospital, Cardiff and Vale NHS Trust, Cardiff CF5 2LD, United Kingdom.
authors.forename: Wasim Sardar
authors.initials: WS
authors.lastname: Khan
authors.affiliation: Wasim Sardar Khan, Jagmeet Bhamra, Rhodri Williams, Rhidian Morgan-Jones, Cardiff and Vale Orthopaedic Centre, Llandough University Hospital, Cardiff and Vale NHS Trust, Cardiff CF5 2LD, United Kingdom.
authors.forename: Jagmeet
authors.initials: J
authors.lastname: Bhamra
authors.affiliation: Wasim Sardar Khan, Jagmeet Bhamra, Rhodri Williams, Rhidian Morgan-Jones, Cardiff and Vale Orthopaedic Centre, Llandough University Hospital, Cardiff and Vale NHS Trust, Cardiff CF5 2LD, United Kingdom.
authors.forename: Rhodri
authors.initials: R
authors.lastname: Williams
authors.affiliation: Wasim Sardar Khan, Jagmeet Bhamra, Rhodri Williams, Rhidian Morgan-Jones, Cardiff and Vale Orthopaedic Centre, Llandough University Hospital, Cardiff and Vale NHS Trust, Cardiff CF5 2LD, United Kingdom.
authors.forename: Rhidian
authors.initials: R
authors.lastname: Morgan-Jones
keywords: Histology; Joint line; Knee; Menical scar; Revision surgery