title: Upregulation of the long non-coding RNA SPRY4-IT1 indicates a poor prognosis and promotes tumorigenesis in ovarian cancer.
abstract: Long non-coding RNAs (lncRNAs) have been identified to be critical mediators in various tumors associated with cancer progression. LncRNA SPRY4-IT1 serves as a novel prognostic biomarker for hepatocellular carcinoma. However, the biological role and clinical significance of lncRNA SPRY4-IT1 in human ovarian cancer (OC) need to be completely elucidated. The aim of the present study was to explore the lncRNA SPRY4-IT1 expression in human OC patients and its role in OC cells. We show that lncRNA SPRY4-IT1 expression is significantly upregulated in ovarian tumor tissues and OC cell lines in comparison with adjacent non-tumor control tissues and the human ovarian immortalized nontumorigenic ovarian surface epithelial (IOSE), respectively. Further analysis by Kaplan-Meier survival analysis and multivariate analysis indicated that high lncRNA SPRY4-IT1 expression may be an independent prognostic factor for progression-free survival (PFS) and overall survival (OS) in OC patients. Furthermore, the area under the receiver operating characteristic (ROC) curve of lncRNA SPRY4-IT1 was up to 0.8512, indicating lncRNA SPRY4-IT1 has diagnostic values to discriminate tumor tissues from nontumorous tissues. Also, knockdown of lncRNA SPRY4-IT1 inhibited the proliferation of OC cells by CCK-8 assay and clonogenic assay and arrested cell cycle at a G0/G1 stage in OC cells. In conclusion, these results suggest that lncRNA SPRY4-IT1 may be considered as a new predictor in the clinical prognosis of OC patients.
authors.affiliation: Department of Gynaecology, the Affiliated Hospital, Yan'an University, Yan'an, Shaanxi Province, 716000, China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Obstetrics, the Maternity & Infant Health Hospital, Baoji, Shaanxi Province, 721000, China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Gynaecology, the Gaoxin Hospital, Xi'an, Shaanxi Province, 710075, China.
authors.forename: Zhanbin
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Department of Obstetrics, the Maternity & Infant Health Hospital, Baoji, Shaanxi Province, 721000, China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Obstetrics, the Affiliated Hospital, Yan'an University, Yan'an, Shaanxi Province, 716000, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Obstetrics, the Affiliated Hospital, Yan'an University, Yan'an, Shaanxi Province, 716000, China.
authors.forename: Yindi
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Gynaecology, No. 215 Hospital of Shaanxi Nuclear Industry, Xianyang, Shaanxi Province, 712000, China. Electronic address: mahuiping19790701@126.com.
authors.forename: Huiping
authors.initials: H
authors.lastname: Ma
mesh_terms: D063646:Carcinogenesis / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D008875:Middle Aged; D015999:Multivariate Analysis; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology*; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D012372:ROC Curve; D015854:Up-Regulation / Q000235:genetics*
chemical_list: D062085:RNA, Long Noncoding; C000605823:long noncoding RNA SPRY4-IT1, human
keywords: Long non-coding RNA; Ovarian cancer; Prognosis; SPRY4-IT1