title: Phased Genome Sequencing Through Chromosome Sorting.
abstract: Phase information of an individual genome provides fundamentally useful genetic information for the understanding of genome function, phenotype, and disease. With the development of new sequencing technology, much interest has been focused on the challenges in obtaining long-range phase information. Here, we present the detailed protocol for a method capable of generating genomic sequences completely phased across the entire chromosome through FACS-mediated chromosome sorting and next generation sequencing, known as Phase-seq.
authors.affiliation: Department of Statistics, Stanford University, Stanford, CA, 94305, USA.|Bio-X Program, Stanford University, Stanford, CA, 94305, USA.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Statistics, Stanford University, Stanford, CA, 94305, USA.|Bio-X Program, Stanford University, Stanford, CA, 94305, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Statistics, Stanford University, Stanford, CA, 94305, USA. whwong@stanford.edu.|Bio-X Program, Stanford University, Stanford, CA, 94305, USA. whwong@stanford.edu.|Department of Health Research and Policy, Stanford University, Stanford, CA, 94305, USA. whwong@stanford.edu.
authors.forename: Wing Hung
authors.initials: WH
authors.lastname: Wong
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D017422:Sequence Analysis, DNA
keywords: Chromosome sorting; Haplotype; Phased sequencing; SNP; Single chromosome sequencing