title: Comparison Between Two Detection Methods for HPV16, HPV18 and P16Ink4a Biomarkers in Diagnosis of Abnormal Cervical Cytology
abstract: Background: Cervical cancer is the second most common cancer among women in many populations. While the
  Pap smear is a well established screening test it suffers from both false-positive and false-negative results in diagnosis
  of cancers and precancerous states. In this study, immunocytochemistry of the P16 biomarker and HPV-PCR were
  compared for their diagnostic potential. Materials and methods: In the study, we obtained pairs of specimens from 45
  women with cervical dysplasia. One sample was placed in a liquid-based solution, and processed for staining of sections
  with antibodies to P16. HPV-PCR was performed on the other and the results obtained were analyzed by T-test using
  SPSS v. 15. Results: Using HPV-PCR 71% of the samples were found to be infected with either HPV 16 or HPV 18,
  and the rate of infection did not have a statistically significant relationship with higher grades of dysplasia (p= 0.253).
  In contrast, with immunocytochemistry evaluation of P16, 64% of the specimens were positive, but the percentage of
  positive results significantly increased with higher grades of dysplasia (p= 0.0001). Conclusion: Employment of the
  P16 marker as an optional test might be preferable over HPV-PCR for cervical dysplasia in our geographical region.
authors.affiliation: Molecular Pathology Research Center, Imam Reza University Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran. Email: Bizadi@hotmail.com
authors.forename: Sedigheh
authors.initials: S
authors.lastname: Khazaei
authors.forename: Babak
authors.initials: B
authors.lastname: Izadi
authors.forename: Seyed Ghasem
authors.initials: SG
authors.lastname: Mirbahari
authors.forename: Seyed Hamid
authors.initials: SH
authors.lastname: Madani
authors.forename: Shohre
authors.initials: Sh
authors.lastname: Malek Khosravi
authors.forename: Mohsen
authors.initials: M
authors.lastname: Emami Alagha
authors.forename: Soraya
authors.initials: S
authors.lastname: Sajadimajd
keywords: Dysplasia; cervix; HPV 16; HPV 18