title: Satellite DNA methylation status and expression of selected genes in Bos indicus blastocysts produced in vivo and in vitro.
abstract: Bovine embryos produced in vivo and in vitro differ with respect to molecular profiles, including epigenetic marks and gene expression profiles. This study investigated the CpG methylation status in bovine testis satellite I (BTS) and Bos taurus alpha satellite I (BTαS) DNA sequences, and concomitantly the relative abundance of transcripts, critically involved in DNA methylation (DNMT1 and DNMT3A), growth and development (IGF2R) and pluripotency (POU5F1) in Bos indicus embryos produced in vitro or in vivo. Results revealed that methylation of BTS were higher (P < 0.05) in embryos produced in vitro compared with their in vivo produced counterparts, while the methylation status of BTαS was similar in both groups. There were no significant differences in transcript abundance for DNMT3A, IGF2R and POU5F1 between blastocysts produced in vivo and in vitro. However, a significantly lower amount of DNMT1 transcripts was found in the in vitro cultured embryos (P < 0.05) compared with their in vivo derived counterparts. In conclusion, this study reported only minor changes in the expression of developmentally important genes and satellite DNA methylation related to the in vitro embryo production system.
authors.affiliation: Grupo INCA-CES,Facultad de Medicina Veterinaria y Zootecnia,Universidad CES,Calle 10 A No. 22-04 MedellínColombia.
authors.forename: R
authors.initials: R
authors.lastname: Urrego
authors.affiliation: Institute of Farm Animal Genetics,Friedrich-Loeffler-Institut (FLI),Höltystraße 10,Mariensee,31535 Neustadt,Germany.
authors.forename: S M
authors.initials: SM
authors.lastname: Bernal-Ulloa
authors.affiliation: Grupo INCA-CES,Facultad de Medicina Veterinaria y Zootecnia,Universidad CES,Calle 10 A No. 22-04 Medellín,Colombia.
authors.forename: N A
authors.initials: NA
authors.lastname: Chavarría
authors.affiliation: Grupo Biología CES-EIA,Universidad CES,Calle 10 A No. 22-04 Medellín,Colombia.
authors.forename: E
authors.initials: E
authors.lastname: Herrera-Puerta
authors.affiliation: Institute of Farm Animal Genetics,Friedrich-Loeffler-Institut (FLI),Höltystraße 10,Mariensee,31535 Neustadt,Germany.
authors.forename: A
authors.initials: A
authors.lastname: Lucas-Hahn
authors.affiliation: Institute of Farm Animal Genetics,Friedrich-Loeffler-Institut (FLI),Höltystraße 10,Mariensee,31535 Neustadt,Germany.
authors.forename: D
authors.initials: D
authors.lastname: Herrmann
authors.affiliation: DNA Sequencing Facility,Max Planck Institute of Molecular Cell Biology and Genetics,Pfotenhauerstraße 108,01307 Dresden,Germany.
authors.forename: S
authors.initials: S
authors.lastname: Winkler
authors.affiliation: DNA Sequencing Facility,Max Planck Institute of Molecular Cell Biology and Genetics,Pfotenhauerstraße 108,01307 Dresden,Germany.
authors.forename: D
authors.initials: D
authors.lastname: Pache
authors.affiliation: Institute of Farm Animal Genetics,Friedrich-Loeffler-Institut (FLI),Höltystraße 10,Mariensee,31535 Neustadt,Germany.
authors.forename: H
authors.initials: H
authors.lastname: Niemann
authors.affiliation: Grupo CENTAURO,Universidad de Antioquia,Calle 70 No. 52-21 Medellín,Colombia.
authors.forename: N
authors.initials: N
authors.lastname: Rodriguez-Osorio
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics*; D019175:DNA Methylation; D004276:DNA, Satellite / Q000235:genetics*; D046149:Embryo Culture Techniques; D005260:Female; D005307:Fertilization in Vitro; D018507:Gene Expression Regulation, Developmental; D066298:In Vitro Techniques; D050814:Octamer Transcription Factor-3 / Q000235:genetics*; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D017527:Receptor, IGF Type 2 / Q000235:genetics*
chemical_list: D004276:DNA, Satellite; D050814:Octamer Transcription Factor-3; D017527:Receptor, IGF Type 2; D004248:DNA (Cytosine-5-)-Methyltransferases
keywords: 
                 In vitro culture; Bovine embryo; DNA methylation; DNMT1; Satellite DNA