title: Automated countercurrent chromatography method development and process scale-up at GlaxoSmithKline.
abstract: The choice of an appropriate combination of stationary phase and mobile phase is essential in any purification technique. To expedite this task, we use automated method screening, typically with high performance liquid chromatography (HPLC), to reduce the time to develop a scalable purification process. We have extended this approach to countercurrent chromatography (CCC) and describe a custom-configured system using automation to execute the solvent screening process. We also present results from three examples of method transfer from analytical to preparative scale using CCC systems from two different manufacturers.
authors.affiliation: GlaxoSmithKline Pharmaceuticals, Analytical Sciences and Development, King of Prussia, PA 19406, USA. Electronic address: david.w.thornton@gsk.com.
authors.forename: David
authors.initials: D
authors.lastname: Thornton
authors.affiliation: GlaxoSmithKline Pharmaceuticals, Analytical Sciences and Development, King of Prussia, PA 19406, USA.
authors.forename: Xiqin
authors.initials: X
authors.lastname: Yang
authors.affiliation: GlaxoSmithKline Pharmaceuticals, Analytical Sciences and Development, King of Prussia, PA 19406, USA.
authors.forename: Leo
authors.initials: L
authors.lastname: Hsu
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003377:Countercurrent Distribution; D004345:Drug Industry / Q000379:methods*; D012997:Solvents / Q000737:chemistry
chemical_list: D012997:Solvents
keywords: CCC; Counter-current chromatography; Countercurrent chromatography; Method scale-up; Method transfer; Solvent system screening