title: Dual colorimetric and fluorogenic probes for visualizing tyrosine phosphatase activity.
abstract: Two resorufin-based substrates for protein tyrosine phosphatase (PTP) activity have been synthesized. These substrates provide sensitive fluorogenic readouts of PTP activity in vitro and in living cells at both acidic and neutral pH. In addition, the presence of the pathogenic bacteria Staphylococcus aureus was detected visually using a colorimetric readout.
authors.affiliation: Department of Medicinal Chemistry, University of Utah College of Pharmacy, Salt Lake City, Utah 84112, USA. amy.barrios@utah.edu.
authors.forename: Suvendu
authors.initials: S
authors.lastname: Biswas
authors.affiliation: Department of Medicinal Chemistry, University of Utah College of Pharmacy, Salt Lake City, Utah 84112, USA. amy.barrios@utah.edu.
authors.forename: Brandon S
authors.identifier: http://orcid.org/0000-0002-4496-0914
authors.initials: BS
authors.lastname: McCullough
authors.affiliation: Department of Medicinal Chemistry, University of Utah College of Pharmacy, Salt Lake City, Utah 84112, USA. amy.barrios@utah.edu.
authors.forename: Elena S
authors.initials: ES
authors.lastname: Ma
authors.affiliation: Department of Oncological Sciences, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA.
authors.forename: Dollie
authors.initials: D
authors.lastname: LaJoie
authors.affiliation: Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA.
authors.forename: Colin W
authors.identifier: http://orcid.org/0000-0003-3942-0996
authors.initials: CW
authors.lastname: Russell
authors.affiliation: Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA.
authors.forename: D
authors.initials: D
authors.lastname: Garrett Brown
authors.affiliation: Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA.
authors.forename: June L
authors.initials: JL
authors.lastname: Round
authors.affiliation: Department of Oncological Sciences, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA.
authors.forename: Katharine S
authors.initials: KS
authors.lastname: Ullman
authors.affiliation: Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA.
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Mulvey
authors.affiliation: Department of Medicinal Chemistry, University of Utah College of Pharmacy, Salt Lake City, Utah 84112, USA. amy.barrios@utah.edu.
authors.forename: Amy M
authors.identifier: http://orcid.org/0000-0003-2739-9261
authors.initials: AM
authors.lastname: Barrios
mesh_terms: D003124:Colorimetry; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000201:enzymology
chemical_list: D005456:Fluorescent Dyes; D017027:Protein Tyrosine Phosphatases