title: Engineered Probiotics: Applications and Biological Containment.
abstract: Bioengineered probiotics represent the next generation of whole cell-mediated biotherapeutics. Advances in synthetic biology, genome engineering, and DNA sequencing and synthesis have enabled scientists to design and develop probiotics with increased stress tolerance and the ability to target specific pathogens and their associated toxins, as well as to mediate targeted delivery of vaccines, drugs, and immunomodulators directly to host cells. Herein, we review the most significant advances in the development of this field. We discuss the critical issue of biological containment and consider the role of synthetic biology in the design and construction of the probiotics of the future.
authors.affiliation: Department of Biological Sciences, Cork Institute of Technology, Bishopstown, Cork, Ireland; email: b.sola-oladokun@mycit.ie , eamonn.culligan@cit.ie , roy.sleator@cit.ie.
authors.forename: Babasola
authors.initials: B
authors.lastname: Sola-Oladokun
authors.affiliation: Department of Biological Sciences, Cork Institute of Technology, Bishopstown, Cork, Ireland; email: b.sola-oladokun@mycit.ie , eamonn.culligan@cit.ie , roy.sleator@cit.ie.
authors.forename: Eamonn P
authors.initials: EP
authors.lastname: Culligan
authors.affiliation: Department of Biological Sciences, Cork Institute of Technology, Bishopstown, Cork, Ireland; email: b.sola-oladokun@mycit.ie , eamonn.culligan@cit.ie , roy.sleator@cit.ie.|APC Microbiome Institute, University College Cork, Cork, Ireland.
authors.forename: Roy D
authors.initials: RD
authors.lastname: Sleator
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D001688:Biological Products / Q000008:administration & dosage; D003264:Containment of Biohazards; D016503:Drug Delivery Systems; D005818:Genetic Engineering; D019936:Probiotics; D058615:Synthetic Biology; D014612:Vaccines / Q000008:administration & dosage
chemical_list: D000276:Adjuvants, Immunologic; D001688:Biological Products; D014612:Vaccines
keywords: biological containment; biotherapeutics; cancer; probiotics; synthetic biology; vaccines