title: Therapeutic properties of Scutellaria baicalensis in db/db mice evaluated using Connectivity Map and network pharmacology.
abstract: We have reported that an extract of Scutellaria baicalensis (ESB) has effects against obesity and hypertriglyceridemia in type 2 diabetic animal model (db/db mouse). In the present study, we tried to explain the pharmacological effects of ESB by integrating gene expression information from db/db mouse liver with that of ESB-treated HepG2 hepatocellular carcinoma cells. Using Connectivity Map (cmap) analysis, we found an inverse relationship in the pharmaceutical profiles based on gene expression between db/db mouse liver and ESB-treated HepG2 cells. This inverse relationship between the two data sets was also observed for pathway activities. Functional network analysis showed that biological functions associated with diabetes and lipid metabolism were commonly enriched in both data sets. We also observed a similarity in distribution of cmap enrichment scores between db/db mouse liver and human diabetic liver, whereas there was an inverse pattern of cmap enrichment scores in human diabetic liver compared with ESB-treated HepG2 cells. This relationship might explain the pharmacological activities of ESB against db/db mouse and possible effectiveness of ESB against human diabetes. We expect that our approach using in vitro cell lines could be applied in predicting the pharmacological effectiveness of herbal drugs in in vivo systems.
authors.affiliation: Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea.
authors.forename: Bu-Yeo
authors.initials: BY
authors.lastname: Kim
authors.affiliation: Mibyeong Research Center, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea.
authors.forename: Kwang Hoon
authors.initials: KH
authors.lastname: Song
authors.affiliation: College of Medicine, Dongguk University, Ilsan, Gyeonggi-do, Republic of Korea.
authors.forename: Chi-Yeon
authors.initials: CY
authors.lastname: Lim
authors.affiliation: School of Korean Medicine, Pusan National University, Yangsan, Republic of Korea.
authors.forename: Su-In
authors.initials: SI
authors.lastname: Cho
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000187:drug effects*; D019295:Computational Biology / Q000379:methods; D003920:Diabetes Mellitus / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D056945:Hep G2 Cells; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D031364:Scutellaria baicalensis / Q000737:chemistry*; D059467:Transcriptome
chemical_list: D001786:Blood Glucose; D010936:Plant Extracts; C499814:Scutellaria baicalensis extract