title: Antibacterial compound produced by Pseudomonas aeruginosa strain UICC B-40, an endophytic bacterium isolated from Neesia altissima.
abstract: This study's aim was to determine the identity of antibacterial compounds produced by Pseudomonas aeruginosa strain UICC B-40 and describe the antibacterial compounds' mechanisms of action for damaging pathogenic bacteria cells. Isolation and identification of the compounds were carried out using thin layer chromatography (TLC), nuclear magnetic resonance (NMR) spectroscopy and liquid chromatography mass spectrometry (LC-MS) analyses. Antibacterial activity was assayed via minimum inhibitory concentration (MIC) and the antibacterial compound mechanism was observed morphologically through scanning electron microscopy (SEM). This study successfully identified the (2E,5E)-phenyltetradeca-2,5-dienoate antibacterial compound (molecular weight 300 g/mol), composed of a phenolic ester, fatty acid and long chain of aliphatic group structures. MIC values for this compound were determined at 62.5 μg/ml against Staphylococcus aureus strain ATCC 25923. The mechanism of the compound involved breaking down the bacterial cell walls through the lysis process. The (2E,5E)-phenyltetradeca-2,5-dienoate compound exhibited inhibitory activity on the growth of Gram-positive bacteria.
authors.affiliation: Department of Biological Education, Faculty of Technics, Mathematics and Natural Sciences, University of Indraprasta PGRI, South Jakarta, 12530, Indonesia. rina.hp2012@gmail.com.|Department of Biology, Faculty of Mathematics and Natural Sciences, University of Indonesia, Depok, 16424, Indonesia. rina.hp2012@gmail.com.
authors.forename: Rina Hidayati
authors.initials: RH
authors.lastname: Pratiwi
authors.affiliation: Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences (LIPI), Jalan Raya Bogor Km 46, Cibinong, 16911, Indonesia.
authors.forename: Iman
authors.initials: I
authors.lastname: Hidayat
authors.affiliation: Research Center for Chemistry, Indonesian Institute of Sciences (LIPI), PUSPIPTEK, Serpong, 15314, Indonesia.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Hanafi
authors.affiliation: Department of Biology, Faculty of Mathematics and Natural Sciences, University of Indonesia, Depok, 16424, Indonesia.
authors.forename: Wibowo
authors.initials: W
authors.lastname: Mangunwardoyo
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001688:Biological Products / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D060026:Endophytes / Q000737:chemistry; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000648:ultrastructure; D009682:Magnetic Resonance Spectroscopy; D019660:Malvaceae / Q000382:microbiology*; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D011550:Pseudomonas aeruginosa / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D001688:Biological Products
keywords: Neesia altissima; Pseudomonas aeruginosa strain UICC B-40; antibacterial; bioactive; endophytic bacteria