title: Effects of neural differentiation maturity status of human induced pluripotent stem cells prior to grafting in a subcortical ischemic stroke model.
abstract: Neural cell grafting is a promising therapy for stroke, but the optimal differentiation status of the cells prior to grafting is unclear. We grafted cells at different maturity stages (days 28, 42, or 56 of in vitro neural differentiation) into the brains of eight-week-old rats one week after subcortical ischemic stroke, and assessed motor and sensory behavioral recovery over one month. We did not find a difference between the grafted or control groups on behavioral recovery, or on brain tissue outcomes including infarct size, microgliosis, or astrocytosis. Further research is needed into mechanisms of benefit of neural cell grafting for stroke.
authors.affiliation: University of Wisconsin Department of Neurology.
authors.forename: Matthew B
authors.initials: MB
authors.lastname: Jensen
authors.affiliation: University of Wisconsin Department of Neurology.
authors.forename: Lindsey D
authors.initials: LD
authors.lastname: Jager
authors.affiliation: University of Wisconsin Department of Neurology.
authors.forename: Laura K
authors.initials: LK
authors.lastname: Cohen
authors.affiliation: University of Wisconsin Department of Neurology.
authors.forename: Susanna S
authors.initials: SS
authors.lastname: Kwok
authors.affiliation: University of Wisconsin Department of Neurology.
authors.forename: Jin M
authors.initials: JM
authors.lastname: Kwon
authors.affiliation: University of Wisconsin Department of Neurology.
authors.forename: Crystal A
authors.initials: CA
authors.lastname: Hall
authors.affiliation: University of Wisconsin Department of Neurology.
authors.forename: Cassandra
authors.initials: C
authors.lastname: Heilingoetter
keywords: differentiation; grafting; human; induced pluripotent stem cells; ischemic stroke; maturity; model; neural; rat; subcortical