title: Dual Fluorescent- and Isotopic-Labelled Self-Assembling Vancomycin for in vivo Imaging of Bacterial Infections.
abstract: The increase of bacterial resistance demands rapid and accurate diagnosis of bacterial infections. Biosurface-induced supramolecular assembly for diagnosis and therapy has received little attention in detecting bacterial infections. Herein we present a dual fluorescent-nuclear probe based on self-assembly of vancomycin (Van) on Gram-positive bacteria for imaging bacterial infection. A Van- and rhodamine-modified peptide derivative (Rho-FF-Van), as the imaging agent, binds to the terminal peptide of the methicillin-resistant staphylococcus aureus (MRSA) and self-assembles to form nanoaggregates on the surface of MRSA. In an in vivo myositis model, Rho-FF-Van results in a significant increased fluorescence signal at the MRSA infected site. Radiolabeled with iodine-125, Rho-FF-Van shows strong radioactive signal in the MRSA-infected lungs in a murine model. This novel dual fluorescent and nuclear probe promises a new way for in vivo imaging of bacterial infections.
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300192, P.R. China.|Department of Chemistry, Brandeis University, Waltham, MA, 02454, USA.
authors.forename: Cuihong
authors.initials: C
authors.lastname: Yang
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300192, P.R. China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Ren
authors.affiliation: Department of Chemistry, Brandeis University, Waltham, MA, 02454, USA.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300192, P.R. China.
authors.forename: Jinjian
authors.initials: J
authors.lastname: Liu
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300192, P.R. China.
authors.forename: Yumin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300192, P.R. China.
authors.forename: Fan
authors.initials: F
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, 300071, P.R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Ding
authors.affiliation: Department of Chemistry, Brandeis University, Waltham, MA, 02454, USA.
authors.forename: Bing
authors.identifier: 0000-0002-4639-387X
authors.initials: B
authors.lastname: Xu
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300192, P.R. China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Liu
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D007457:Iodine Radioisotopes / Q000737:chemistry; D008168:Lung / Q000000981:diagnostic imaging; D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification*; D008958:Models, Molecular; D009220:Myositis / Q000000981:diagnostic imaging / Q000382:microbiology; D061848:Optical Imaging / Q000379:methods; D011859:Radiography / Q000379:methods; D012235:Rhodamines / Q000737:chemistry*; D013203:Staphylococcal Infections / Q000000981:diagnostic imaging* / Q000382:microbiology; D014640:Vancomycin / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D007457:Iodine Radioisotopes; D012235:Rhodamines; D014640:Vancomycin; C000614960:Iodine-125
keywords: bacterial infections; fluorescent probes; in vivo imaging; isotopic labeling; self-assembly; vancomycin