title: In Vitro Antifungal Susceptibility of Oral Candida Isolates from Patients Suffering from Caries and Chronic Periodontitis.
abstract: Caries and chronic periodontitis are common oral diseases where a higher Candida colonization is reported. Antifungal agents could be adjuvant drugs for the therapy of both clinical conditions. The aim of the current study has been to evaluate the in vitro activities of conventional and new antifungal drugs against oral Candida isolates from patients suffering from caries and/or chronic periodontitis. In vitro activities of amphotericin B, fluconazole, itraconazole, miconazole, nystatin, posaconazole and voriconazole against 126 oral Candida isolates (75 Candida albicans, 18 Candida parapsilosis, 11 Candida dubliniensis, six Candida guilliermondii, five Candida lipolytica, five Candida glabrata, four Candida tropicalis and two Candida krusei) from 61 patients were tested by the CLSI M27-A3 method. Most antifungal drugs were highly active, and resistance was observed in less than 5% of tested isolates. Miconazole was the most active antifungal drug, being more than 98% of isolates susceptible. Fluconazole, itraconazole, and the new triazoles, posaconazole and voriconazole, were also very active. Miconazole, fluconazole and voriconazole have excellent in vitro activities against all Candida isolates and could represent suitable treatment for a hypothetically adjunctive therapy of caries and chronic periodontitis.
authors.affiliation: Laboratorio de Micología Médica, Departamento de Inmunología, Microbiología y Parasitología, UFI 11/25, Facultad de Medicina y Enfermería, Universidad del País Vasco-Euskal Herriko Unibertsitatea, Apartado 699, 48080, Bilbao, Spain.|Unidad de Medicina Bucal, Servicio Clínica Odontológica, Departamento de Estomatología II, UFI 11/25, Facultad de Medicina y Enfermería, Universidad del País Vasco-Euskal Herriko Unibertsitatea, Bilbao, Spain.
authors.forename: Janire
authors.identifier: http://orcid.org/0000-0003-0438-2248
authors.initials: J
authors.lastname: De-la-Torre
authors.affiliation: Laboratorio de Micología Médica, Departamento de Inmunología, Microbiología y Parasitología, UFI 11/25, Facultad de Medicina y Enfermería, Universidad del País Vasco-Euskal Herriko Unibertsitatea, Apartado 699, 48080, Bilbao, Spain.
authors.forename: María Esther
authors.initials: ME
authors.lastname: Ortiz-Samperio
authors.affiliation: Laboratorio de Micología Médica, Departamento de Inmunología, Microbiología y Parasitología, UFI 11/25, Facultad de Medicina y Enfermería, Universidad del País Vasco-Euskal Herriko Unibertsitatea, Apartado 699, 48080, Bilbao, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Marcos-Arias
authors.affiliation: Unidad de Medicina Bucal, Servicio Clínica Odontológica, Departamento de Estomatología II, UFI 11/25, Facultad de Medicina y Enfermería, Universidad del País Vasco-Euskal Herriko Unibertsitatea, Bilbao, Spain.
authors.forename: Xabier
authors.initials: X
authors.lastname: Marichalar-Mendia
authors.affiliation: Laboratorio de Micología Médica, Departamento de Inmunología, Microbiología y Parasitología, UFI 11/25, Facultad de Medicina y Enfermería, Universidad del País Vasco-Euskal Herriko Unibertsitatea, Apartado 699, 48080, Bilbao, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Eraso
authors.affiliation: Unidad de Medicina Bucal, Servicio Clínica Odontológica, Departamento de Estomatología II, UFI 11/25, Facultad de Medicina y Enfermería, Universidad del País Vasco-Euskal Herriko Unibertsitatea, Bilbao, Spain.
authors.forename: María Ángeles
authors.initials: MÁ
authors.lastname: Echebarria-Goicouria
authors.affiliation: Unidad de Medicina Bucal, Servicio Clínica Odontológica, Departamento de Estomatología II, UFI 11/25, Facultad de Medicina y Enfermería, Universidad del País Vasco-Euskal Herriko Unibertsitatea, Bilbao, Spain.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Aguirre-Urizar
authors.affiliation: Laboratorio de Micología Médica, Departamento de Inmunología, Microbiología y Parasitología, UFI 11/25, Facultad de Medicina y Enfermería, Universidad del País Vasco-Euskal Herriko Unibertsitatea, Apartado 699, 48080, Bilbao, Spain. guillermo.quindos@ehu.eus.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Quindós
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects* / Q000302:isolation & purification; D055113:Chronic Periodontitis / Q000382:microbiology*; D003731:Dental Caries / Q000382:microbiology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D009055:Mouth / Q000382:microbiology*; D011446:Prospective Studies
chemical_list: D000935:Antifungal Agents
keywords: Amphotericin B; Antifungal susceptibility; Candida; Caries; Chronic periodontitis; Fluconazole; Itraconazole; Miconazole; Nystatin; Oral; Posaconazole; Voriconazole