title: Fluorinated trimethyllysine as a19F NMR probe for trimethyllysine hydroxylase catalysis.
abstract: Trimethyllysine hydroxylase (TMLH) catalyses C-3 hydroxylation of Nε-trimethyllysine in the first step of carnitine biosynthesis in humans. Studies on TMLH have been hampered by the lack of established chemical methods. We report that an Nε-trimethyllysine analogue that contains the fluoromethyl group can be used as a1H and19F NMR probe for studies on TMLH catalysis.
authors.affiliation: Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands. j.mecinovic@science.ru.nl.
authors.forename: Y
authors.initials: Y
authors.lastname: Vijayendar Reddy
authors.affiliation: Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands. j.mecinovic@science.ru.nl.
authors.forename: Abbas H K
authors.initials: AH
authors.lastname: Al Temimi
authors.affiliation: Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands. j.mecinovic@science.ru.nl.
authors.forename: Jasmin
authors.identifier: http://orcid.org/0000-0002-5559-3822
authors.initials: J
authors.lastname: Mecinović
mesh_terms: D055162:Biocatalysis; D005461:Fluorine; D054879:Halogenation; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D015335:Molecular Probes / Q000096:biosynthesis* / Q000737:chemistry; D015394:Molecular Structure
chemical_list: D015335:Molecular Probes; D005461:Fluorine; C003712:trimethyllysine; D006899:Mixed Function Oxygenases; C025931:trimethyl-lysine hydroxylase; D008239:Lysine