title: Mechanistic insights into the impact of Cold Atmospheric Pressure Plasma on human epithelial cell lines.
abstract: Compelling evidence suggests that Cold Atmospheric Pressure Plasma (CAPP) has potential as a new cancer therapy. However, knowledge about cellular signaling events and toxicity subsequent to plasma treatment is still poorly documented. The aim of this study was to focus on the interaction between 3 different types of plasma (He, He-O2, He-N2) and human epithelial cell lines to gain better insight into plasma-cell interaction. We provide evidence that reactive oxygen and nitrogen species (RONS) are inducing cell death by apoptosis and that the proteasome, a major intracellular proteolytic system which is important for tumor cell growth and survival, is a target of (He or He-N2) CAPP. However, RONS are not the only actors involved in cell death; electric field and charged particles could play a significant role especially for He-O2CAPP. By differential label-free quantitative proteomic analysis we found that CAPP triggers antioxidant and cellular defense but is also affecting extracellular matrix in keratinocytes. Moreover, we found that malignant cells are more resistant to CAPP treatment than normal cells. Taken together, our findings provide insight into potential mechanisms of CAPP-induced proteasome inactivation and the cellular consequences of these events.
authors.affiliation: IPREM, UMR 5254, Université de Pau et des Pays de l'Adour, 64000, Pau, France.
authors.forename: Marlène
authors.initials: M
authors.lastname: Dezest
authors.affiliation: IPREM, UMR 5254, Université de Pau et des Pays de l'Adour, 64000, Pau, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Chavatte
authors.affiliation: STROMALAB, Université de Toulouse, CNRS ERL5311, EFS, INP-ENVT, UPS, INSERM U1031, BP31432 Toulouse cedex 4, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Bourdens
authors.affiliation: Univ. BORDEAUX, ISM. CNRS UMR 5255 NSysA group, ENSCBP, Pessac, 33607, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Quinton
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, UPS, France.
authors.forename: Mylène
authors.initials: M
authors.lastname: Camus
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, UPS, France.
authors.forename: Luc
authors.initials: L
authors.lastname: Garrigues
authors.affiliation: Genoskin, Centre Pierre Potier-Oncopole, F-31106 Toulouse, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Descargues
authors.affiliation: Univ. BORDEAUX, ISM. CNRS UMR 5255 NSysA group, ENSCBP, Pessac, 33607, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Arbault
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, UPS, France.
authors.forename: Odile
authors.initials: O
authors.lastname: Burlet-Schiltz
authors.affiliation: STROMALAB, Université de Toulouse, CNRS ERL5311, EFS, INP-ENVT, UPS, INSERM U1031, BP31432 Toulouse cedex 4, France.
authors.forename: Louis
authors.initials: L
authors.lastname: Casteilla
authors.affiliation: IPREM, UMR 5254, Université de Pau et des Pays de l'Adour, 64000, Pau, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Clément
authors.affiliation: STROMALAB, Université de Toulouse, CNRS ERL5311, EFS, INP-ENVT, UPS, INSERM U1031, BP31432 Toulouse cedex 4, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Planat
authors.affiliation: IPREM, UMR 5254, Université de Pau et des Pays de l'Adour, 64000, Pau, France.
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Bulteau
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis; D002460:Cell Line; D005109:Extracellular Matrix / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006371:Helium / Q000494:pharmacology*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism; D058626:Plasma Gases / Q000494:pharmacology*; D011312:Pressure; D046988:Proteasome Endopeptidase Complex / Q000187:drug effects; D020543:Proteome / Q000187:drug effects / Q000235:genetics / Q000378:metabolism
chemical_list: D000975:Antioxidants; D058626:Plasma Gases; D020543:Proteome; D006371:Helium; D046988:Proteasome Endopeptidase Complex