title: Minimal amino acids in the I/LWEQ domain required for anterior/posterior localization in Dictyostelium.
abstract: Establishment of cell polarity is mediated by a series of signaling molecules that are asymmetrically activated or localized in the cell upon extracellular stimulation. To understand the mechanism that mediates anterior/posterior asymmetric localization of RapGAP3 during migration, we determined the minimally required amino acids in the I/LWEQ domain that cause posterior localization and found that the minimal region of the F-actin binding domain for posterior localization could, with some additional deletion at the C-terminal, localize to the anterior. Analysis of the localization and translocation kinetics to the cell cortex of the truncated proteins suggests that the required regions for anterior/posterior localization might have a preferential binding affinity to preexisting F-actins at the rear and lateral sides of the cell or newly formed F-actins at the front of the cell, leading to distinct differential sites of the cell.
authors.affiliation: Department of Biology & BK21-Plus Research Team for Bioactive Control Technology, College of Natural Sciences, Chosun University, Gwangju, 61452, Republic of Korea.
authors.forename: Hyeseon
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Biology & BK21-Plus Research Team for Bioactive Control Technology, College of Natural Sciences, Chosun University, Gwangju, 61452, Republic of Korea.
authors.forename: Dong-Yeop
authors.initials: DY
authors.lastname: Shin
authors.affiliation: Department of Biology & BK21-Plus Research Team for Bioactive Control Technology, College of Natural Sciences, Chosun University, Gwangju, 61452, Republic of Korea. tjeon@chosun.ac.kr.
authors.forename: Taeck Joong
authors.initials: TJ
authors.lastname: Jeon
mesh_terms: D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry* / Q000235:genetics; D000818:Animals; D002465:Cell Movement; D016764:Cell Polarity; D017124:Conserved Sequence; D003599:Cytoskeleton / Q000378:metabolism; D004023:Dictyostelium / Q000737:chemistry / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D017434:Protein Structure, Tertiary; D020827:rap1 GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D000199:Actins; D000596:Amino Acids; D020827:rap1 GTP-Binding Proteins
keywords: Dictyostelium; I/LWEQ domain; RapGAP3; cell migration; cell polarity