title: Up regulation of MMP9 gene expression in female patients with multiple sclerosis.
abstract: BACKGROUND
  Multiple sclerosis (MS) is an inflammatory, immune-mediated, demyelinating disease of the central nervous system which disturbs cerebral vascular permeability.
  
  
  OBJECTIVE
  Matrix metalloproteinase can increase capillary permeability and are involved in neurological diseases.
  
  
  METHODS
  The study compared the expression level of MMP9 gene in RRMS samples with normal individuals in Iran. RNA from the buffy coat of 50 RRMS patients and 50 normal controls were extracted. All patients were HLA-DRB1*15 negative and were responders to interferon-beta with a normal vitamin D level. The level of MMP9 gene expression was measured using quantitative RT-PCR.
  
  
  RESULTS
  The RRMS patients manifested a higher expression level of MMP9 than their normal counterparts (P= 0.02). Age-wise, there was no correlation between different age groups (> 30, 30-40, 40 <). In terms of sex, only the female patients manifested a statistically significant increase in MMP9 (p value = 0.037). Besides, there was no linear correlation between MMP9 expression level and risk of Expanded Disability Status Scale of Kurtzke (EDSS); nor were there any significant correlation between expression status of MMP9 and duration of the disease.
  
  
  CONCLUSIONS
  Up regulation of MMP9 gene expression would happen in RRMS IFN-B responders, but the level of increase in female patients is significantly more than males.
authors.affiliation: Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Shima Yazdandoost
authors.initials: SY
authors.lastname: Hamedani
authors.affiliation: Department of Medical Genetics, School of Medicine Sciences, IShahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Taheri
authors.affiliation: Department of Hematology, School of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran,Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Sajjadi
authors.affiliation: Department of Medical Genetics, School of Medicine Sciences, IShahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Mir Davood
authors.initials: MD
authors.lastname: Omrani
authors.affiliation: Department of Neurology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Mehrdokht
authors.initials: M
authors.lastname: Mazdeh
authors.affiliation: Department of Epidemiology and Biostatistics, Faculty of Health, Qom University of Medical Sciences, Qom, Iran.
authors.forename: Shahram
authors.initials: S
authors.lastname: Arsang-Jang
authors.affiliation: Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Akram Sadat Tabatabaei
authors.initials: AS
authors.lastname: Panah
authors.affiliation: Department of Medical Genetics, School of Medicine Sciences, IShahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Arezou
authors.initials: A
authors.lastname: Sayad
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D059811:HLA-DRB1 Chains / Q000235:genetics / Q000276:immunology; D006801:Humans; D007155:Immunologic Factors / Q000627:therapeutic use; D016899:Interferon-beta / Q000627:therapeutic use; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics* / Q000276:immunology; D008875:Middle Aged; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000188:drug therapy / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D012737:Sex Factors; D015854:Up-Regulation; D014807:Vitamin D / Q000097:blood
chemical_list: D059811:HLA-DRB1 Chains; C507799:HLA-DRB1*15 antigen; D007155:Immunologic Factors; D014807:Vitamin D; D016899:Interferon-beta; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: IFN-B; MMP9; expression; multiple sclerosis