title: miR-187 induces apoptosis of SiHa cervical carcinoma cells by downregulating Bcl-2.
abstract: Cervical carcinoma is a life-threatening illness posing considerable danger to women's health. microRNAs (miRNAs) have been shown to regulate multiple cellular events, including growth and proliferation, and miR-187 is thought to regulate the growth and apoptosis of certain cell types. Our study focused on the influence of miR-187 on the growth, proliferation, and apoptosis of SiHa cervical carcinoma cells, and explored the mechanism behind its pro-apoptotic effect. miR-187 and control (scrambled) miRNA were synthesized with a standard protocol and lipofected into SiHa cells. Thiazolyl blue tetrazolium bromide assays and tests of caspase-3 activity were then performed to examine growth, proliferation, and apoptosis by flow cytometry. Small interfering RNA (siRNA) and an expression plasmid were synthesized for inhibition and overexpression of Bcl-2, respectively, and following their transfection, western blotting was used to examine Bcl-2 protein levels. Compared to transfection with control miRNA, miR-187 significantly reduced SiHa cell growth and decreased Bcl-2 expression. Increased translocation of phosphatidylserine and activation of caspase-3 were observed in miR-187-transfected cells. Moreover, inhibition of Bcl-2 enhanced the pro-apoptotic effect of this miRNA, while Bcl-2 overexpression had the opposite effect. miR-187 inhibits the growth and proliferation of SiHa cells, and induces their apoptosis via downregulation of Bcl-2. Bcl-2 represents a potential therapeutic target for cervical carcinoma.
authors.affiliation: Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, China.|Central Hospital of Wuhan University, Enshi Autonomous Prefecture, Enshi City, Hubei Province, China.
authors.forename: C Y
authors.initials: CY
authors.lastname: He
authors.affiliation: Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, China jingyangzxc@sina.com.
authors.forename: J
authors.initials: J
authors.lastname: Yang
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D034622:RNA Interference; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
chemical_list: C571843:MIRN187 microRNA, human; D035683:MicroRNAs; D019253:Proto-Oncogene Proteins c-bcl-2