title: Eugenol prevents amyloid formation of proteins and inhibits amyloid-induced hemolysis.
abstract: Eugenol has attracted considerable attention because of its potential for many pharmaceutical applications including anti-inflammatory, anti-tumorigenic and anti-oxidant properties. Here, we have investigated the effect of eugenol on amyloid formation of selected globular proteins. We find that both spontaneous and seed-induced aggregation processes of insulin and serum albumin (BSA) are significantly suppressed in the presence of eugenol. Isothermal titration calorimetric data predict a single binding site for eugenol-insulin complex confirming the affinity of eugenol for native soluble insulin species. We also find that eugenol suppresses amyloid-induced hemolysis. Our findings reveal the inherent ability of eugenol to stabilize native proteins and to delay the conversion of protein species of native conformation into β-sheet assembled mature fibrils, which seems to be crucial for its inhibitory effect.
authors.affiliation: Department of Biology, Indian Institute of Technology Jodhpur, Rajasthan, 342011 India.
authors.forename: Kriti
authors.initials: K
authors.lastname: Dubey
authors.affiliation: Department of Biology, Indian Institute of Technology Jodhpur, Rajasthan, 342011 India.
authors.forename: Bibin G
authors.initials: BG
authors.lastname: Anand
authors.affiliation: Department of Biology, Indian Institute of Technology Jodhpur, Rajasthan, 342011 India.
authors.forename: Dolat Singh
authors.initials: DS
authors.lastname: Shekhawat
authors.affiliation: Department of Biology, Indian Institute of Technology Jodhpur, Rajasthan, 342011 India.|School of Life Sciences, Jawaharlal Nehru University, New Delhi-110067, India.
authors.forename: Karunakar
authors.initials: K
authors.lastname: Kar
mesh_terms: D000682:Amyloid / Q000378:metabolism*; D058227:Amyloidogenic Proteins / Q000378:metabolism*; D000686:Amyloidosis; D005054:Eugenol / Q000378:metabolism / Q000494:pharmacology*; D006461:Hemolysis; D006801:Humans; D018625:Microscopy, Atomic Force; D066329:Protein Aggregates / Q000187:drug effects*; D066263:Protein Aggregation, Pathological; D011485:Protein Binding; D013057:Spectrum Analysis
chemical_list: D000682:Amyloid; D058227:Amyloidogenic Proteins; D066329:Protein Aggregates; D005054:Eugenol