title: In Vitro and In Vivo Approaches to Assess Rho Kinase Activity.
abstract: Increased arterial tone and the resulting rise in peripheral vascular resistance are major determinants of the elevated arterial pressure in hypertension. The RhoA/Rho kinase signaling pathways are now recognized as a major regulator of vascular smooth muscle contraction and arterial tone. Here we describe methods to directly and indirectly assess Rho kinase activity in vitro and in cells and tissues.
authors.affiliation: INSERM, CNRS, UNIV Nantes, l'institut du thorax, 8 Quai Moncousu, BP 70721, 44007, Nantes Cedex 1, France.|CHU de Nantes, Nantes, 44000, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Sauzeau
authors.affiliation: INSERM, CNRS, UNIV Nantes, l'institut du thorax, 8 Quai Moncousu, BP 70721, 44007, Nantes Cedex 1, France. gervaise.loirand@univ-nantes.fr.|CHU de Nantes, Nantes, 44000, France. gervaise.loirand@univ-nantes.fr.
authors.forename: Gervaise
authors.initials: G
authors.lastname: Loirand
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000235:genetics / Q000502:physiology; D015153:Blotting, Western; D006801:Humans; D006973:Hypertension / Q000235:genetics / Q000378:metabolism; D047468:Immunoprecipitation; D066298:In Vitro Techniques; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000378:metabolism; D043263:Myosin-Light-Chain Phosphatase / Q000378:metabolism; D054460:rho-Associated Kinases / Q000235:genetics / Q000378:metabolism*; D020742:rhoA GTP-Binding Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: D054460:rho-Associated Kinases; D043263:Myosin-Light-Chain Phosphatase; D020742:rhoA GTP-Binding Protein
keywords: Immunoblot; In vitro kinase assay; Myosin light-chain phosphatase; Rho kinase