title: Sigma 2 receptor expression levels in blood and bladder from healthy and bladder cancer cattle.
abstract: The expression of sigma-2 receptor (S2R) was assayed in blood and bladder samples from healthy cattle and in blood and bladder of cattle with deltapapillomavirus-associated urothelial tumors. Samples of bladder from cattle with neoplasia had significantly higher S2R than samples of bladder from healthy cattle (95% CI 0.31-0.82, P < 0.05). In addition, significantly higher S2R was detected in the blood of cattle with bladder cancer than blood from healthy cattle (95% CI 0.22-0.41, P < 0.05). The results provide evidence that increased expression of SR2 in blood could be useful as circulating biomarker for bladder cancer in cattle. PGRMC1 protein levels were also found to be increased in blood and bladder from cattle with cancer and increased expression of PGRMC1 transcripts was detected by quantitative real time PCR in samples from cattle neoplasia. Furthermore, electron microscopy revealed phagophores and numerous autophagosomes, ultrastructural hallmark of autophagy.
authors.affiliation: Dipartimento di Medicina Veterinaria e Produzioni Animali, Università di Napoli Federico II, Napoli, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Russo
authors.affiliation: Dipartimento di Farmacia-Scienze del Farmaco, Università di Bari, Bari, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Inglese
authors.affiliation: Dipartimento di Medicina Veterinaria e Produzioni Animali, Università di Napoli Federico II, Napoli, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Avallone
authors.affiliation: Dipartimento di Biologia, Università di Napoli Federico II, Napoli, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Roperto
authors.affiliation: Dipartimento di Farmacia-Scienze del Farmaco, Università di Bari, Bari, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Abate
authors.affiliation: Dipartimento di Medicina Veterinaria, Università di Bari, Bari, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Zizzo
authors.affiliation: College of Science, Massey University, Palmerston North, New Zealand.
authors.forename: J S
authors.initials: JS
authors.lastname: Munday
authors.affiliation: Dipartimento di Farmacia-Scienze del Farmaco, Università di Bari, Bari, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Berardi
authors.affiliation: Dipartimento di Farmacia-Scienze del Farmaco, Università di Bari, Bari, Italy.
authors.forename: N A
authors.initials: NA
authors.lastname: Colabufo
authors.affiliation: Dipartimento di Medicina Veterinaria e Produzioni Animali, Università di Napoli Federico II, Napoli, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Roperto
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D015153:Blotting, Western / Q000662:veterinary; D016022:Case-Control Studies; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission / Q000662:veterinary; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D017480:Receptors, sigma / Q000097:blood / Q000378:metabolism*; D001743:Urinary Bladder / Q000378:metabolism / Q000648:ultrastructure; D001749:Urinary Bladder Neoplasms / Q000097:blood / Q000378:metabolism / Q000662:veterinary*
chemical_list: D015415:Biomarkers; D017480:Receptors, sigma; C106107:sigma-2 receptor
keywords: PGRMC1 protein; autophagy; bladder cancer; deltapapillomavirus; sigma 2 receptor