title: Super RLuc8-sFv; a new luciferase-labeled probe for detection of human CD4+ cells.
abstract: The human immunodeficiency virus (HIV) destroys CD4+ lymphocytes and monitoring these cells is one of the best techniques for studying HIV infection. In the present study a novel bioluminescent probe, super RLuc8-sFv, is developed in order to detect human CD4+ cells by fusion of an anti-human CD4 sFv to the C-terminus of super RLuc8. The results indicate that the probe can bind to CD4+ cells via its sFv domain; also it emits visible light through its signalling domain. Super RLuc8-sFv provides a new gateway for detection of human CD4+ cells using luminometric-based assays and may reduce the difficulties involved in, and the cost of, HIV-related diagnostic tests.
authors.affiliation: Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran. Sci_rahman@yahoo.com r.emamzadeh@sci.ui.ac.ir.
authors.forename: A
authors.initials: A
authors.lastname: Safavi
authors.affiliation: Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran. Sci_rahman@yahoo.com r.emamzadeh@sci.ui.ac.ir.
authors.forename: R
authors.identifier: http://orcid.org/0000-0002-7924-1135
authors.initials: R
authors.lastname: Emamzadeh
authors.affiliation: Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Nazari
authors.affiliation: Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran. Sci_rahman@yahoo.com r.emamzadeh@sci.ui.ac.ir.
authors.forename: M
authors.initials: M
authors.lastname: Ehsani
authors.affiliation: Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran. Sci_rahman@yahoo.com r.emamzadeh@sci.ui.ac.ir.
authors.forename: S H
authors.initials: SH
authors.lastname: Zarkesh-Esfahani
authors.affiliation: Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran. Sci_rahman@yahoo.com r.emamzadeh@sci.ui.ac.ir.
authors.forename: S
authors.initials: S
authors.lastname: Rahgozar
mesh_terms: D015704:CD4 Antigens / Q000737:chemistry / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D006801:Humans; D008156:Luciferases / Q000737:chemistry; D008163:Luminescent Measurements / Q000379:methods; D008164:Luminescent Proteins / Q000737:chemistry; D008958:Models, Molecular; D015335:Molecular Probes / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D057127:Single-Chain Antibodies / Q000737:chemistry
chemical_list: D015704:CD4 Antigens; D008164:Luminescent Proteins; D015335:Molecular Probes; D057127:Single-Chain Antibodies; D008156:Luciferases