title: Mixed chimerism renders residual host dendritic cells incapable of alloimmunization of the marrow donor in the canine model of allogeneic marrow transplantation.
abstract: This study tested whether an alloimmune response can occur in the marrow donor when infused or injected with leukocytes from their mixed chimeric transplant recipient. Two mixed chimeras were produced after conditioning with three Gray total body irradiation, donor marrow infusion, and post-grafting immunosuppression. The marrow donors were then repeatedly infused and injected with leukocytes from their respective chimeric recipient. A donor lymphocyte infusion (DLI) into their mixed chimeras had no effect, even after the experiments were repeated. The presence of blood dendritic cells (DCs) of recipient origin was confirmed in chimeric recipients, as well as the presence of microchimerism in the marrow donors. Donor sensitization did occur following placement of a recipient skin graft that was confirmed following DLI into recipients that changed the mixed chimeras into full donor chimeras. These observations suggest that mixed chimerism renders recipient peripheral blood DCs incapable of inducing a donor T cell response.
authors.affiliation: a Division of Clinical Research, Fred Hutchinson Cancer Research Center , Seattle , WA , USA.|b Department of Medicine , University of Washington , Seattle , WA , USA.
authors.forename: Steven L
authors.initials: SL
authors.lastname: Rosinski
authors.affiliation: a Division of Clinical Research, Fred Hutchinson Cancer Research Center , Seattle , WA , USA.|b Department of Medicine , University of Washington , Seattle , WA , USA.
authors.forename: Scott S
authors.initials: SS
authors.lastname: Graves
authors.affiliation: a Division of Clinical Research, Fred Hutchinson Cancer Research Center , Seattle , WA , USA.
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Higginbotham
authors.affiliation: a Division of Clinical Research, Fred Hutchinson Cancer Research Center , Seattle , WA , USA.|b Department of Medicine , University of Washington , Seattle , WA , USA.
authors.forename: Rainer
authors.initials: R
authors.lastname: Storb
mesh_terms: D064591:Allografts; D000818:Animals; D016026:Bone Marrow Transplantation; D003713:Dendritic Cells / Q000276:immunology*; D004285:Dogs; D005260:Female; D006084:Graft Rejection / Q000276:immunology*; D017710:Lymphocyte Transfusion; D008297:Male; D018448:Models, Immunological; D016038:Skin Transplantation; D018183:Transplantation Chimera / Q000276:immunology*
keywords: alloimmunization; dendritic cells; microchimerism; minor histocompatibility antigens; mixed chimerism; tolerance