title: Development of Quantum Dots-Labeled Antibody Fluorescence Immunoassays for the Detection of Morphine.
abstract: Quantum dots (QDs)-labeled antibody fluorescence immunoassays (FLISA) for the detection of morphine were developed. Quantum dots (CdSe/ZnS), which contained carboxyl, were used to label antimorphine antibody by 1-ethyl-3-(3-dimethylaminoprophyl) carbodiimide hydrochloride/N-hydroxysulfosuccinimide, which were used as coupling reagents. The CdSe/ZnS QDs labeled antimorphine antibody (QDs labeled Ab) was characterized by fluorescence spectrum and gel electrophoresis. Plate-based FLISA and nitrocellulose membrane-based flow-through FLISA were developed and applied to quantitative and qualitative detection of morphine. Under the optimal conditions for plate-based FLISA, the linear range spanned from 3.2 × 10-4to 1 mg/L (R2= 0.9905), and the detection limit was 2.7 × 10-4mg/L. The visual detection limit for morphine by membrane-based flow-through FLISA was 0.01 mg/L. These results demonstrated that the developed fluorescence immunoassays could be applied as highly sensitive and convenient tools for rapid detection of morphine, which make it ideally suited for on-site screening of poppy shell added illegally in hot pot soup base.
authors.affiliation: School of Food & Biological Engineering, Jiangsu University , Zhenjiang 212013, P. R. China.
authors.forename: Can
authors.identifier: http://orcid.org/0000-0003-4505-0119
authors.initials: C
authors.lastname: Zhang
authors.affiliation: School of Food & Biological Engineering, Jiangsu University , Zhenjiang 212013, P. R. China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Han
authors.affiliation: School of Food & Biological Engineering, Jiangsu University , Zhenjiang 212013, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.affiliation: School of Food & Biological Engineering, Jiangsu University , Zhenjiang 212013, P. R. China.
authors.forename: Nannan
authors.initials: N
authors.lastname: Deng
authors.affiliation: Zhenjiang Institute for Drug Control, Zhenjiang 212000, P. R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.affiliation: Zhenjiang Institute for Drug Control, Zhenjiang 212000, P. R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry; D019187:Cadmium Compounds / Q000737:chemistry; D005455:Fluorescent Antibody Technique / Q000295:instrumentation / Q000379:methods; D005504:Food Analysis / Q000379:methods*; D006241:Haptens / Q000737:chemistry; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D057230:Limit of Detection; D009020:Morphine / Q000032:analysis* / Q000276:immunology; D045663:Quantum Dots; D011817:Rabbits; D018036:Selenium Compounds / Q000737:chemistry; D013388:Succinimides; D013440:Sulfides / Q000737:chemistry; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: D000906:Antibodies; D019187:Cadmium Compounds; D006241:Haptens; D018036:Selenium Compounds; D013388:Succinimides; D013440:Sulfides; D017967:Zinc Compounds; D009020:Morphine; C035761:N-hydroxysulfosuccinimide; C058667:cadmium selenide; C031238:zinc sulfide
keywords: flow-through; fluorescence immunoassay; morphine; nitrocellulose membrane; quantum dots