title: Sulforaphene attenuates multinucleation of pre-osteoclasts by suppressing expression of cell-cell fusion-associated genes DC-STAMP, OC-STAMP, and Atp6v0d2.
abstract: We assessed the effect of sulforaphene (SFE) on osteoclast differentiation. SFE significantly decreased the number of RANKL-induced tartrate-resistant acid phosphatase-positive cells and suppressed pre-osteoclast multinucleation. Furthermore, SFE downregulated mRNA expression of DC-STAMP, OC-STAMP, and Atp6v0d2, which encode cell-cell fusion molecules. Our data suggest that SFE attenuates pre-osteoclast multinucleation via suppression of cell-cell fusion.
authors.affiliation: a Faculty of Applied Bioscience, Department of Nutritional Science and Food Safety , Tokyo University of Agriculture , Tokyo , Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Takagi
authors.affiliation: a Faculty of Applied Bioscience, Department of Nutritional Science and Food Safety , Tokyo University of Agriculture , Tokyo , Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Inoue
authors.affiliation: a Faculty of Applied Bioscience, Department of Nutritional Science and Food Safety , Tokyo University of Agriculture , Tokyo , Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Takahashi
authors.affiliation: a Faculty of Applied Bioscience, Department of Nutritional Science and Food Safety , Tokyo University of Agriculture , Tokyo , Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Katsumata-Tsuboi
authors.affiliation: a Faculty of Applied Bioscience, Department of Nutritional Science and Food Safety , Tokyo University of Agriculture , Tokyo , Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Uehara
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002459:Cell Fusion; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects; D005786:Gene Expression Regulation; D017879:Isothiocyanates / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008565:Membrane Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D053245:RANK Ligand / Q000494:pharmacology; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D025262:Vacuolar Proton-Translocating ATPases / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism
chemical_list: C499636:DC-STAMP protein, mouse; D017879:Isothiocyanates; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C527519:OC-STAMP protein, mouse; D053245:RANK Ligand; D012333:RNA, Messenger; C505689:Tnfsf11 protein, mouse; C542312:Atp6v0d2 protein, mouse; D025262:Vacuolar Proton-Translocating ATPases; C473643:sulphoraphene
keywords: dendritic cell-specific transmembrane protein (DC-STAMP); isothiocyanate; osteoclast cell fusion; sulforaphene; v-ATPase V0 subunit d2(Atp6v0d2)