title: An innovative ring-shaped electroeluter for high concentration preparative isolation of protein from polyacrylamide gel.
abstract: A ring-shaped electroeluter (RSE) was designed for protein recovery from polyacrylamide gel matrix. The RSE was designed in such a way that a ring-shaped well was used to place gel slices and an enrichment well was used to collect eluted protein samples. With HSA as model protein, the electroelution time was less than 30 min with 80% recovery rate, and the concentration of recovered protein was 50 times higher than that of conventional method. The RSE could be reused at least ten times. The developed device makes great advance towards economic electroelution of biomolecules (such as proteins) from gel matrix.
authors.affiliation: Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Chen
authors.affiliation: Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Guo-Qing
authors.initials: GQ
authors.lastname: Li
authors.affiliation: Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Amit
authors.initials: A
authors.lastname: Pratush
authors.affiliation: Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Sharmin
authors.initials: S
authors.lastname: Jahan
authors.affiliation: Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Fan-Zhi
authors.initials: FZ
authors.lastname: Kong
authors.affiliation: Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Xiao
authors.affiliation: Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: lyfan@sjtu.edu.cn.
authors.forename: Liu-Yin
authors.initials: LY
authors.lastname: Fan
authors.affiliation: Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: cxcao@sjtu.edu.cn.
authors.forename: Cheng-Xi
authors.initials: CX
authors.lastname: Cao
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D012709:Serum Albumin / Q000302:isolation & purification*
chemical_list: D000180:Acrylic Resins; D012709:Serum Albumin; C016680:polyacrylamide gels
keywords: Electroelution; Gel electrophoresis; Preparative isolation; Protein; Sample recovery