title: Polycomb-dependent nucleolus localization of Jumonji/Jarid2 duringDrosophilaspermatogenesis.
abstract: DrosophilaJumonji/Jarid2 (dJmj) has been identified as a component of Polycomb repressive complex 2. However, it is suggested that dJmj has both PRC-dependent and -independent roles. Subcellular localization of dJmj during spermatogenesis is unknown. We therefore performed immunocytochemical analyses with specific antibodies to dJmj and tri-methylation at lysine 27 on histone H3 (H3K27me3). Interestingly, dJmj exclusively localizes at nucleolus in the late growth stage. Examination of the dJmj localization in various Polycomb group (PcG) mutant lines at the late growth stage allowed identification of some PcG genes, including Polycomb (Pc), to be responsible for dJmj recruitment to nucleolus. In addition, we found that size of nucleolus was decreased in some of these mutant lines. In a mutant of testis-specific TAF homolog (tTAF) that is responsible for nucleolus localization of Pc, dJmj signals were detected not only at nucleolus but also on the condensed chromatin in the late growth stage. DuolinkIn situProximity ligation assay clarified that Pc interacts with dJmj at nucleolus in the late growth stage. Furthermore, the level of H3K27me3 decreased in nuclei at this stage. Taken together, we conclude that tTAF is responsible for recruitments of dJmj to nucleolus in the late growth stage that appears to be mediated by Pc. Compartmentalization of dJmj in nucleolus together with some of PcG may be necessary to de-repress the expression of genes required to cellular growth and proliferation in the following meiotic divisions.
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology , Kyoto, Japan.
authors.forename: Moyu
authors.initials: M
authors.lastname: Goto
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology , Kyoto, Japan.
authors.forename: Narumi
authors.initials: N
authors.lastname: Toda
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology , Kyoto, Japan.
authors.forename: Kouhei
authors.initials: K
authors.lastname: Shimaji
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology , Kyoto, Japan.
authors.forename: Dang Ngoc Anh
authors.initials: DN
authors.lastname: Suong
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology , Kyoto, Japan.
authors.forename: Nicole
authors.initials: N
authors.lastname: Vo
authors.affiliation: Institute of Innovative Research (IIR), Tokyo Institute of Technology , Yokohama, Japan.
authors.forename: Hiroshi
authors.identifier: 0000-0003-0854-083X
authors.initials: H
authors.lastname: Kimura
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology , Kyoto, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Yoshida
authors.affiliation: Insect Advanced Research Center, Kyoto Institute of Technology , Kyoto, Japan.
authors.forename: Yoshihiro H
authors.initials: YH
authors.lastname: Inoue
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology, Kyoto, Japan; Insect Advanced Research Center, Kyoto Institute of Technology, Kyoto, Japan.
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Yamaguchi
keywords: Drosophila; Jumonji/Jarid2; Polycomb group protein; epigenetics; spermatogenesis; subcellular localization