title: Enhanced Identification of Group B Streptococcus and Escherichia Coli in Young Infants with Meningitis Using the Biofire Filmarray Meningitis/Encephalitis Panel.
abstract: FilmArray Meningitis/Encephalitis (ME) polymerase chain reaction (PCR) panel was tested on 62 cerebrospinal fluid (CSF) samples from young infants (0-3 months) with suspected meningitis and compared with CSF cultures. Twelve CSF samples from 9 infants were positive by ME PCR panel (10 Group B Streptococcus (GBS) and 2 Escherichia coli) of which only 5 were positive by culture. The 7 CSF samples that were positive only by ME PCR panel were obtained from infants who had received prior antibiotic treatment. The ME PCR panel can be a useful tool in the rapid diagnosis of bacterial meningitis in pretreated young infants.
authors.affiliation: From the *Division of Infectious Diseases, Children's Hospital of Michigan, Detroit, Michigan; †Carman and Ann Adams Department of Pediatrics, Wayne State University, Detroit, Michigan; ‡Department of Pathology, Wayne State University, Detroit, Michigan; §Detroit Medical Center University Laboratories, Detroit, Michigan; and ¶Division of Neonatal Perinatal Medicine, Children's Hospital of Michigan, Detroit, Michigan.
authors.forename: Harbir Singh
authors.initials: HS
authors.lastname: Arora
authors.forename: Basim I
authors.initials: BI
authors.lastname: Asmar
authors.forename: Hossein
authors.initials: H
authors.lastname: Salimnia
authors.forename: Prashant
authors.initials: P
authors.lastname: Agarwal
authors.forename: Sanjay
authors.initials: S
authors.lastname: Chawla
authors.forename: Nahed
authors.initials: N
authors.lastname: Abdel-Haq
mesh_terms: D015331:Cohort Studies; D004926:Escherichia coli / Q000235:genetics*; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D016920:Meningitis, Bacterial / Q000175:diagnosis* / Q000382:microbiology; D008824:Michigan; D058889:Molecular Typing / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013290:Streptococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013292:Streptococcus agalactiae / Q000235:genetics*