title: Expression of thermostable β-xylosidase in Escherichia coli for use in saccharification of plant biomass.
abstract: The present work is aimed to evaluate the saccharification potential of a thermostable β-xylosidase cloned from Bacillus licheniformis into Escherichia coli for production of bioethanol from plant biomass. Recombinant β-xylosidase enzyme possesses the ability of bioconversion of plant biomass like wheat straw, rice straw and sugarcane bagass. By using this approach, plant biomass that mainly constitute cellulose can be converted to reducing sugars that could then be easily converted to bioethanol by simple fermentation process. The production of bioethanol will help to overcome energy requirements due to depleting fossil fuels and will also help to protect environment by reducing greenhouse gas emission. In the end, future directions are briefly mentioned that can be utilized to reduce the cost and increase the yield of biofuels.
authors.affiliation: a Institute of Industrial Biotechnology, Government College University , Katchery Road, Lahore , Pakistan.
authors.forename: Muhammad N
authors.initials: MN
authors.lastname: Aftab
authors.affiliation: a Institute of Industrial Biotechnology, Government College University , Katchery Road, Lahore , Pakistan.
authors.forename: Asma
authors.initials: A
authors.lastname: Zafar
authors.affiliation: b Institute of Biochemistry & Biotechnology, University of Veterinary and Animal Sciences , Lahore , Pakistan.
authors.forename: Ali R
authors.initials: AR
authors.lastname: Awan
mesh_terms: D000069976:Bacillus licheniformis / Q000201:enzymology* / Q000235:genetics; D004789:Enzyme Activation; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D000431:Ethanol / Q000302:isolation & purification / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods; D035261:Plant Components, Aerial / Q000382:microbiology*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013696:Temperature; D014995:Xylosidases / Q000235:genetics / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D000431:Ethanol; D014995:Xylosidases; C026579:exo-1,4-beta-D-xylosidase
keywords: E. coli; expression; plant biomass; saccharification; xylosidase