title: The aliphatic amidase AmiE is involved in regulation of Pseudomonas aeruginosa virulence.
abstract: We have previously shown that the eukaryotic C-type natriuretic peptide hormone (CNP) regulates Pseudomonas aeruginosa virulence and biofilm formation after binding on the AmiC sensor, triggering the amiE transcription. Herein, the involvement of the aliphatic amidase AmiE in P. aeruginosa virulence regulation has been investigated. The proteome analysis of an AmiE over-producing strain (AmiE+) revealed an expression change for 138 proteins, including some that are involved in motility, synthesis of quorum sensing compounds and virulence regulation. We observed that the AmiE+strain produced less biofilm compared to the wild type, and over-produced rhamnolipids. In the same line, AmiE is involved in P. aeruginosa motilities (swarming and twitching) and production of the quorum sensing molecules N-acyl homoserine lactones and Pseudomonas Quinolone Signal (PQS). We observed that AmiE overproduction reduced levels of HCN and pyocyanin causing a decreased virulence in different hosts (i.e. Dictyostelium discoideum and Caenorhabditis elegans). This phenotype was further confirmed in a mouse model of acute lung infection, in which AmiE overproduction resulted in an almost fully virulence decrease. Taken together, our data suggest that, in addition to its role in bacterial secondary metabolism, AmiE is involved in P. aeruginosa virulence regulation by modulating pilus synthesis and cell-to-cell communication.
authors.affiliation: Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Clamens
authors.affiliation: Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.
authors.forename: Thibaut
authors.initials: T
authors.lastname: Rosay
authors.affiliation: Univ. Bretagne-Sud, EA 3884, LBCM, IUEM, Lorient, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Crépin
authors.affiliation: Univ. Lille, CHU Lille, EA 7366 - Recherche Translationnelle: relations hôte pathogènes, Lille, France.
authors.forename: Teddy
authors.initials: T
authors.lastname: Grandjean
authors.affiliation: Laboratory « Polymères, Biopolymères, Surfaces » (UMR 6270 CNRS), Proteomic Platform PISSARO, Normandie Univ, UNIROUEN, Mont-Saint-Aignan, France.
authors.forename: Takfarinas
authors.initials: T
authors.lastname: Kentache
authors.affiliation: Laboratory « Polymères, Biopolymères, Surfaces » (UMR 6270 CNRS), Proteomic Platform PISSARO, Normandie Univ, UNIROUEN, Mont-Saint-Aignan, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Hardouin
authors.affiliation: Univ. Lille, CHU Lille, EA 7366 - Recherche Translationnelle: relations hôte pathogènes, Lille, France.
authors.forename: Perrine
authors.initials: P
authors.lastname: Bortolotti
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute of Functional Interfaces, PO Box 3640, Karlsruhe, Germany.
authors.forename: Anke
authors.initials: A
authors.lastname: Neidig
authors.affiliation: BIOMERIT Research Centre, University College Cork, Cork, Ireland.
authors.forename: Marlies
authors.initials: M
authors.lastname: Mooij
authors.affiliation: Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Hillion
authors.affiliation: Normandie Univ, UNIROUEN, INSA Rouen, CNRS, COBRA (UMR 6014), Evreux, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Vieillard
authors.affiliation: Laboratory « Polymères, Biopolymères, Surfaces » (UMR 6270 CNRS), Proteomic Platform PISSARO, Normandie Univ, UNIROUEN, Mont-Saint-Aignan, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Cosette
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute of Functional Interfaces, PO Box 3640, Karlsruhe, Germany.
authors.forename: Joerg
authors.initials: J
authors.lastname: Overhage
authors.affiliation: BIOMERIT Research Centre, University College Cork, Cork, Ireland.|School of Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, Perth, Australia.
authors.forename: Fergal
authors.initials: F
authors.lastname: O'Gara
authors.affiliation: Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.
authors.forename: Emeline
authors.initials: E
authors.lastname: Bouffartigues
authors.affiliation: Univ. Bretagne-Sud, EA 3884, LBCM, IUEM, Lorient, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Dufour
authors.affiliation: Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Chevalier
authors.affiliation: Univ. Lille, CHU Lille, EA 7366 - Recherche Translationnelle: relations hôte pathogènes, Lille, France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Guery
authors.affiliation: Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Cornelis
authors.affiliation: Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.
authors.forename: Marc G J
authors.initials: MG
authors.lastname: Feuilloley
authors.affiliation: Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Lesouhaitier
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism*; D000818:Animals; D018441:Biofilms; D017173:Caenorhabditis elegans / Q000382:microbiology; D004023:Dictyostelium / Q000382:microbiology; D005260:Female; D008168:Lung / Q000382:microbiology; D008297:Male; D008810:Mice, Inbred C57BL; D020543:Proteome; D011552:Pseudomonas Infections / Q000201:enzymology* / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000472:pathogenicity*; D053038:Quorum Sensing; D014774:Virulence; D037521:Virulence Factors
chemical_list: D020543:Proteome; D037521:Virulence Factors; D000581:Amidohydrolases; C019665:amidase