title: The synthesis of15N(7)-Hoogsteen face-labeled adenosine phosphoramidite for solid-phase RNA synthesis.
abstract: We have developed an efficient route for the synthesis of15N(7)-labeled adenosine as phosphoramidite building block for site- and atom-specific incorporation into RNA by automated solid-phase synthesis. Such labeled RNA is required for the evaluation of selected non-canonical base pair interactions in folded RNA using NMR spectroscopic methods.
authors.affiliation: Institute of Organic Chemistry, Leopold-Franzens University, Innrain 80-82, Innsbruck, Austria.
authors.forename: Sandro
authors.initials: S
authors.lastname: Neuner
authors.affiliation: Institute of Organic Chemistry, Leopold-Franzens University, Innrain 80-82, Innsbruck, Austria.
authors.forename: Christoph
authors.initials: C
authors.lastname: Kreutz
authors.affiliation: Institute of Organic Chemistry, Leopold-Franzens University, Innrain 80-82, Innsbruck, Austria.
authors.forename: Ronald
authors.initials: R
authors.lastname: Micura
keywords: Base triplet; Modifications; Nucleosides; RNA labeling; RNA structure; Twister ribozyme