title: Phosphatidylethanolamine Deficiency Impairs Escherichia coli Adhesion by Downregulating Lipopolysaccharide Synthesis, Which is Reversible by High Galactose/Lactose Cultivation.
abstract: As the initiation step of bacterial infection or biofouling, bacterial adhesion on cells or substrates is generally an optimal target for antibacterial design. Phosphatidylethanolamine (PE) is the principal phospholipid in bacteria, and its function in bacterial adhesion remains unclear. In this study, four E. coli strains including two PE-deficient mutants (PE-PC-and PE-PC+strains) and two PE-containing wild-type controls (PE+PC-strains) were recruited to investigate the influence of PE deficiency on bacterial adhesion. We found that PE deficiency could impair E. coli adhesion on macrophages (human THP-1-derived and mouse RAW264.7 macrophages) or glass coverslips by downregulating lipopolysaccharide (LPS) biosynthesis, which could be reversible by high galactose/lactose but not glucose cultivation. The data imply that PE play important role in bacterial adhesion probably via affecting LPS biosynthesis and suggest that targeting PE biosynthesis is also a potential antibacterial strategy.
authors.affiliation: a College of Life Sciences, Nanchang University , Nanchang , Jiangxi , P.R. China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Yu
authors.affiliation: a College of Life Sciences, Nanchang University , Nanchang , Jiangxi , P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: b Nanoscale Science and Technology Laboratory, Institute for Advanced Study, Nanchang University , Nanchang , Jiangxi , P.R. China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: c Faculty of Life Sciences , Hubei University , Wuchang , Hubei , P.R. China.
authors.forename: Xingguo
authors.initials: X
authors.lastname: Wang
authors.affiliation: a College of Life Sciences, Nanchang University , Nanchang , Jiangxi , P.R. China.|b Nanoscale Science and Technology Laboratory, Institute for Advanced Study, Nanchang University , Nanchang , Jiangxi , P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
mesh_terms: D019138:Antibodies, Blocking / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects*; D002460:Cell Line; D015536:Down-Regulation / Q000187:drug effects*; D004926:Escherichia coli / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development; D005690:Galactose / Q000494:pharmacology*; D005898:Glass / Q000737:chemistry; D006801:Humans; D007785:Lactose / Q000494:pharmacology*; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008070:Lipopolysaccharides / Q000096:biosynthesis*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D010714:Phosphatidylethanolamines / Q000172:deficiency*; D014867:Water / Q000737:chemistry
chemical_list: D019138:Antibodies, Blocking; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D010714:Phosphatidylethanolamines; D014867:Water; C483858:phosphatidylethanolamine; D007785:Lactose; D005690:Galactose
keywords: Escherichia coli; bacterial adhesion; high sugar cultivation; lipopolysaccharide; macrophage; phosphatidylethanolamine