title: miR520c blocks EMT progression of human breast cancer cells by repressing STAT3.
abstract: Breast cancer is one of the most malignant diseases world-wide and it ranks the first among female cancers. Masses of intrinsic and extrinsic factors, especially the inflammatory factors can lead to breast cancer. Aberrant activation and accumulation of key molecules can lead to inflammation associated carcinogenesis. The signal transducers and activators of transcription 3 (STAT3) is one of them. Therefore, to evaluate the novel molecular mechanisms, STAT3 has become our focus for breast cancer targeted therapy. At present, many tumor suppressing microRNAs have been validated, and are the highlights in research on microRNAs. Thus, we predicted microRNAs which could putatively regulate STAT3 through databases and selected six to screen with Dual-luciferase assay. The result hinted that miR520c could bind with STAT3 3'UTR. We mutated the seed sequence of miR520c on STAT3 3'UTR, which illustrated a reverse effect compared with wild-type of STAT3 3'UTR. Subsequently, STAT3, p-STAT3 and miR520c were assessed in three different grades of breast cancer cells, with the degree of malignancy, we found an escalating trend of STAT3 and p-STAT3, on the contrary, a downward trend of miR520c. We observed STAT3 was deactivated by miR520c. Epithelial to mesenchymal transition (EMT) is a fatal transfer of cancer progression. To find out whether the downregulation of STAT3 can repress breast cancer motility and invasion ability, we detected EMT markers. The result implied a suppression effect on EMT. We overexpressed STAT3 to conduct rescue experiments, the result showed a recovery of STAT3 and EMT characteristics. Cell motility and invasion property were regained as well. In the study, we elucidated miR520c could inhibit breast cancer EMT by targeting STAT3. It can enrich the mechanism of breast cancer and may lay the foundation for breast cancer targeted treatment.
authors.affiliation: Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China.
authors.forename: Nian
authors.initials: N
authors.lastname: Wang
authors.affiliation: Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China.
authors.forename: Lan
authors.initials: L
authors.lastname: Wei
authors.affiliation: Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China.
authors.forename: Yunxiu
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Su
authors.affiliation: Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China.
authors.forename: Xueli
authors.initials: X
authors.lastname: Pang
authors.affiliation: Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China.
authors.forename: Nian
authors.initials: N
authors.lastname: Wang
authors.affiliation: Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China.
authors.forename: Feihu
authors.initials: F
authors.lastname: Ji
authors.affiliation: Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China.
authors.forename: Changli
authors.initials: C
authors.lastname: Zhong
authors.affiliation: Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China.
authors.forename: Tingmei
authors.initials: T
authors.lastname: Chen
authors.affiliation: Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology / Q000517:prevention & control*; D002465:Cell Movement; D049109:Cell Proliferation; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050796:STAT3 Transcription Factor / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
chemical_list: C575048:MIRN520 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human