title: Microarray-Based Immunoassay for Parallel Quantification of Multiple Mycotoxins in Oat.
abstract: Because multianalyte methods are highly desirable in order to keep analysis time and costs low, the biosensor development increasingly focuses on parallel analysis of several mycotoxins. Here, we describe an indirect competitive immunoassay on regenerable, reusable glass microchips for the parallel determination of aflatoxins, ochratoxin A, deoxynivalenol, and fumonisin B1 in oat extracts, using a fully automated flow-through device with chemiluminescence readout.
authors.affiliation: Institute of Hydrochemistry and Chair of Analytical Chemistry, Technical University Munich, Marchioninistrasse 17, 81377, Munich, Germany.
authors.forename: Susanna
authors.initials: S
authors.lastname: Oswald
authors.affiliation: Chair of Hygiene and Technology of Milk, Ludwig-Maximilians University Munich, Schönleutnerstrasse 8/219, 85764, Oberschleissheim, Germany.
authors.forename: Richard
authors.initials: R
authors.lastname: Dietrich
authors.affiliation: Chair of Hygiene and Technology of Milk, Ludwig-Maximilians University Munich, Schönleutnerstrasse 8/219, 85764, Oberschleissheim, Germany.
authors.forename: Erwin
authors.initials: E
authors.lastname: Märtlbauer
authors.affiliation: Institute of Hydrochemistry and Chair of Analytical Chemistry, Technical University Munich, Marchioninistrasse 17, 81377, Munich, Germany.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Niessner
authors.affiliation: Institute of Hydrochemistry and Chair of Analytical Chemistry, Technical University Munich, Marchioninistrasse 17, 81377, Munich, Germany. dietmar.knopp@ch.tum.de.
authors.forename: Dietmar
authors.initials: D
authors.lastname: Knopp
mesh_terms: D000906:Antibodies / Q000276:immunology; D057205:Automation, Laboratory; D018554:Avena / Q000382:microbiology*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods; D046228:Microarray Analysis / Q000379:methods*; D009183:Mycotoxins / Q000032:analysis* / Q000737:chemistry
chemical_list: D000906:Antibodies; D009183:Mycotoxins
keywords: Automated multianalyte immunoassay; Chemiluminescence microarray readout; Munich chip reader (MCR 3); Mycotoxins; Oat