title: Detection of metal induced cytopathological alterations and DNA damage in the gills and hepatopancreas of green mussel Perna viridis from Ennore Estuary, Chennai, India.
abstract: This study report the impact of heavy metals on cytopathology and DNA damage in the gills and hepatopancreas of Perna viridis collected from Ennore estuary and the Kovalam coastal waters. Principal Component Analysis (PCA) showed significant differences among all variables at the scale of plots. The ultrastructural alterations such as lack of microvilli, distorted mitochondria, electron dense particles and the presence of large mucous droplets were common in the gill and hepatopancreatic cells of mussels from Ennore estuary. However, the gill and hepatopancreatic cells of P. viridis from Kovalam revealed normal compartmentalization of cells. The percentage of tail DNA in the mussels from Ennore estuary was recorded as 12.44 and 10.14% in the gills and hepatopancreas respectively. Overall, it has been demonstrated that the Comet and cytopathological assays are useful biomarkers to assess the level of pollution and it provide reliable information on ecotoxicology and genotoxicology of coastal waters.
authors.affiliation: Department of Environmental Biotechnology, School of Environmental Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamilnadu, India. Electronic address: arockiavasanthi@gmail.com.
authors.forename: Lourduraj A
authors.initials: LA
authors.lastname: Vasanthi
authors.affiliation: Department of Environmental Biotechnology, School of Environmental Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamilnadu, India.
authors.forename: Peranandam
authors.initials: P
authors.lastname: Revathi
authors.affiliation: Department of Environmental Biotechnology, School of Environmental Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamilnadu, India.
authors.forename: Ramaswamy
authors.initials: R
authors.lastname: Babu Rajendran
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, Tamilnadu, India.
authors.forename: Natesan
authors.initials: N
authors.lastname: Munuswamy
mesh_terms: D000818:Animals; D004249:DNA Damage; D004784:Environmental Monitoring; D063366:Estuaries; D005880:Gills / Q000737:chemistry / Q000473:pathology*; D043143:Hepatopancreas / Q000737:chemistry / Q000473:pathology*; D007194:India; D049883:Perna / Q000166:cytology*; D014874:Water Pollutants, Chemical
chemical_list: D014874:Water Pollutants, Chemical
keywords: Biomarker; Cytopathology; DNA damage; Heavy metals; Perna viridis; TEM