title: Cry1Ac and Vip3Aa proteins fromBacillus thuringiensistargeting Cry toxin resistance inDiatraea flavipennellaandElasmopalpus lignosellusfrom sugarcane.
abstract: The biological potential of Vip and Cry proteins fromBacillusis well known and widely established. Thus, it is important to look for new genes showing different modes of action, selecting those with differentiated entomotoxic activity againstDiatraea flavipennellaandElasmopalpus lignosellus, which are secondary pests of sugarcane. Therefore, Cry1 and Vip3 proteins were expressed inEscherichia coli, and their toxicities were evaluated based on bioassays using neonate larvae. Of those, the most toxic were Cry1Ac and Vip3Aa considering the LC50values. Toxins fromE. coliwere purified, solubilized, trypsinized, and biotinylated. Brush Border Membrane Vesicles (BBMVs) were prepared from intestines of the two species to perform homologous and heterologous competition assays. The binding assays demonstrated interactions between Cry1Aa, Cry1Ac, and Vip3Aa toxins and proteins from the BBMV ofD. flavipennellaandE. lignosellus. Homologous competition assays demonstrated that binding to one of the BBMV proteins was specific for each toxin. Heterologous competition assays indicated that Vip3Aa was unable to compete for Cry1Ac toxin binding. Our results suggest that Cry1Ac and Vip3Aa may have potential in future production of transgenic sugarcane for control ofD. flavipennellaandE. lignosellus, but more research is needed on the potential antagonism or synergism of the toxins in these pests.
authors.affiliation: Department of Biologia Aplicada á Agropecuária, Universidade Estadual Paulista, Jaboticabal, São Paulo, Brazil.
authors.forename: Ana Rita Nunes
authors.initials: ARN
authors.lastname: Lemes
authors.affiliation: Department of Biologia Aplicada á Agropecuária, Universidade Estadual Paulista, Jaboticabal, São Paulo, Brazil.
authors.forename: Camila Soares
authors.initials: CS
authors.lastname: Figueiredo
authors.affiliation: Department of Biologia Aplicada á Agropecuária, Universidade Estadual Paulista, Jaboticabal, São Paulo, Brazil.
authors.forename: Isis
authors.initials: I
authors.lastname: Sebastião
authors.affiliation: Department of Agronomia, Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil.
authors.forename: Liliane
authors.initials: L
authors.lastname: Marques da Silva
authors.affiliation: Department of Agronomia, Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil.
authors.forename: Rebeka
authors.initials: R
authors.lastname: da Costa Alves
authors.affiliation: Department of Agronomia, Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil.
authors.forename: Herbert Álvaro Abreu
authors.initials: HÁA
authors.lastname: de Siqueira
authors.affiliation: Department of Biologia Aplicada á Agropecuária, Universidade Estadual Paulista, Jaboticabal, São Paulo, Brazil.
authors.forename: Manoel Victor Franco
authors.initials: MVF
authors.lastname: Lemos
authors.affiliation: Departamento de Fitossanidade/FCAV/UNESP, Universidade Estadual Paulista, Jaboticabal, São Paulo, Brazil.
authors.forename: Odair Aparecido
authors.initials: OA
authors.lastname: Fernandes
authors.affiliation: Department of Biologia Aplicada á Agropecuária, Universidade Estadual Paulista, Jaboticabal, São Paulo, Brazil.
authors.forename: Janete Apparecida
authors.initials: JA
authors.lastname: Desidério
keywords: Biological control; Competition assay; Insecticidal proteins