title: Protective effects of rambutan (Nephelium lappaceum) peel phenolics on H2O2-induced oxidative damages in HepG2 cells and d-galactose-induced aging mice.
abstract: Rambutan peel phenolic (RPP) extracts were prepared via dynamic separation with macroporous resin. The total phenolic content and individual phenolics in RPP were determined. Results showed that the total phenolic content of RPP was 877.11 mg gallic acid equivalents (GAE)/g extract. The content of geranin (122.18 mg/g extract) was the highest among those of the 39 identified phenolic compounds. RPP protected against oxidative stress in H2O2-induced HepG2 cells in a dose-response manner. The inhibitory effects of RPP on cell apoptosis might be related to its inhibitory effects on the generation of intracellular reactive oxygen species and increased effects on superoxide dismutase activity. The in vivo anti-aging activity of RPP was evaluated using an aging mice model that was induced by d-galactose (d-gal). The results showed that RPP enhanced the antioxidative status of experimental mice. Moreover, histological analysis indicated that RPP effectively reduced d-gal-induced liver and kidney tissue damage in a dose-dependent manner. Therefore, RPP can be used as a natural antioxidant and anti-aging agent in the pharmaceutical and food industries.
authors.affiliation: Yunnan Institute of Food Safety, Kunming University of Science and Technology, No. 727 South Jingming Road, Kunming, Yunnan 650500, China.
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Zhuang
authors.affiliation: Yunnan Institute of Food Safety, Kunming University of Science and Technology, No. 727 South Jingming Road, Kunming, Yunnan 650500, China.
authors.forename: Qingyu
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Yunnan Institute of Food Safety, Kunming University of Science and Technology, No. 727 South Jingming Road, Kunming, Yunnan 650500, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Yunnan Institute of Food Safety, Kunming University of Science and Technology, No. 727 South Jingming Road, Kunming, Yunnan 650500, China. Electronic address: lpsun@kmust.edu.cn.
authors.forename: Liping
authors.initials: L
authors.lastname: Sun
mesh_terms: D000375:Aging / Q000187:drug effects*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D005638:Fruit / Q000737:chemistry; D005690:Galactose / Q000633:toxicity*; D056945:Hep G2 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D051379:Mice; D018384:Oxidative Stress; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species; D029631:Sapindaceae / Q000737:chemistry*
chemical_list: D010636:Phenols; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D005690:Galactose
keywords: HepG2 cells; Histological analysis; Oxidative stress; Rambutan peel phenolics; UPLC-MS/MS; d-galactose-induced aging mice