title: The gut microbiota of siblings offers insights into microbial pathogenesis of inflammatory bowel disease.
abstract: Siblings of patients with Crohn's disease (CD) have elevated risk of developing CD and display aspects of disease phenotype, including faecal dysbiosis. In our recent article we have used 16S rRNA gene targeted high-throughput sequencing to comprehensively characterize the mucosal microbiota in healthy siblings of CD patients, and determine the influence of genotypic and phenotypic factors on the gut microbiota (dysbiosis). We have demonstrated that the core microbiota of both patients with CD and healthy siblings is significantly less diverse than controls. Faecalibacterium prausnitzii contributed most to core metacommunity dissimilarity between both patients and controls and between siblings and controls. Phenotype/genotype markers of CD risk significantly influenced microbiota variation between and within groups, of which genotype had the largest effect. Individuals with elevated CD-risk display mucosal dysbiosis characterized by reduced diversity of core microbiota and lower abundance of F. prausnitzii. The presence of this dysbiosis in healthy people at-risk of CD implicates microbiological processes in CD pathogenesis.
authors.affiliation: a Department of Medicine, Karolinska University Hospital , Solna, Stockholm.
authors.forename: Charlotte R
authors.initials: CR
authors.lastname: Hedin
authors.affiliation: b Manchester Metropolitan University , School of Healthcare Science , Manchester , UK.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: van der Gast
authors.affiliation: c Queen Mary University of London , Centre for Immunobiology, Blizard Institute , London , UK.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Stagg
authors.affiliation: c Queen Mary University of London , Centre for Immunobiology, Blizard Institute , London , UK.|d Barts Health NHS Trust , Department of Gastroenterology , London , UK.
authors.forename: James O
authors.initials: JO
authors.lastname: Lindsay
authors.affiliation: e King's College London , Faculty of Life Sciences & Medicine, Diabetes and Nutritional Sciences Division , London , UK.
authors.forename: Kevin
authors.initials: K
authors.lastname: Whelan
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D064806:Dysbiosis / Q000382:microbiology; D005260:Female; D000069196:Gastrointestinal Microbiome; D005838:Genotype; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000382:microbiology*; D008297:Male; D010641:Phenotype; D035781:Siblings
keywords: Crohn's disease; Faecalibacterium prausnitzii; bifidobacteria; inflammatory bowel disease; microbiome; siblings