title: Vernalization-Triggered Intragenic Chromatin Loop Formation by Long Noncoding RNAs.
abstract: Long noncoding RNAs (lncRNAs) affect gene regulation through structural and regulatory interactions with associated proteins. The Polycomb complex often binds to lncRNAs in eukaryotes, and an lncRNA, COLDAIR, associates with Polycomb to mediate silencing of the floral repressor FLOWERING LOCUS C (FLC) during the process of vernalization in Arabidopsis. Here, we identified an additional Polycomb-binding lncRNA, COLDWRAP. COLDWRAP is derived from the repressed promoter of FLC and is necessary for the establishment of the stable repressed state of FLC by vernalization. Both COLDAIR and COLDWRAP are required to form a repressive intragenic chromatin loop at the FLC locus by vernalization. Our results indicate that vernalization-mediated Polycomb silencing is coordinated by lncRNAs in a cooperative manner to form a stable repressive chromatin structure.
authors.affiliation: Department of Molecular Biosciences, Institute for Cellular and Molecular Biology, The University of Texas at Austin, 2506 Speedway, Austin, TX 78712, USA.
authors.forename: Dong-Hwan
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Department of Molecular Biosciences, Institute for Cellular and Molecular Biology, The University of Texas at Austin, 2506 Speedway, Austin, TX 78712, USA. Electronic address: sbsung@austin.utexas.edu.
authors.forename: Sibum
authors.initials: S
authors.lastname: Sung
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002843:Chromatin / Q000737:chemistry* / Q000378:metabolism; D003080:Cold Temperature; D035264:Flowers / Q000235:genetics* / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D056426:Genetic Loci; D006657:Histones / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D026161:MADS Domain Proteins / Q000235:genetics; D008745:Methylation; D009154:Mutation / Q000235:genetics; D009690:Nucleic Acid Conformation; D030821:Plants, Genetically Modified; D063151:Polycomb Repressive Complex 2; D011401:Promoter Regions, Genetic; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D012097:Repressor Proteins / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C000620725:COLDWRAP long noncoding RNA, Arabidopsis; D002843:Chromatin; C418902:At5g10140 protein, Arabidopsis; D006657:Histones; D026161:MADS Domain Proteins; C574170:PRC2 protein, Arabidopsis; D062085:RNA, Long Noncoding; D012097:Repressor Proteins; D063151:Polycomb Repressive Complex 2; D008239:Lysine
keywords: chromatin loop; flowering; long noncoding RNAs; polycomb repressive complex 2; vernalization