title: Co-Delivery of Docetaxel and p44/42 MAPK siRNA Using PSMA Antibody-Conjugated BSA-PEI Layer-by-Layer Nanoparticles for Prostate Cancer Target Therapy.
abstract: How to overcome the low accumulation of chemotherapeutic agent in tumor tissue and exhibit multitherapeutics remains an ongoing challenge for cancer treatment. Here, a simple method is demonstrated that used to prepare prostate-specific membrane antigen antibody (PSMAab)-conjugated fluorescent bovine serum albumin (BSA)-branched polyethylenimine layer-by-layer nanoparticles (BSA-PEILBLNPs) for co-delivery of docetaxel (DTX) and p44/42 mitogen-activated protein kinase (MAPK) small interfering RNA (p44/42 MAPK siRNA) as synergistic and selective inhibition of cancer cell proliferation platform. The results show the levels of α-tubulin and p44/42 MAPK in CWR22R cells are significantly reduced after treatment with PSMAab-conjugated DTX/BSA-PEILBL/siRNA NPs. Consequently, the 50% cellular growth inhibition (IC50) values of the NPs loaded with both DTX and p44/42 MAPK siRNA are ≈2.1-fold less than those for the NPs only loaded with DTX. The median survival significantly prolongs from 18 d to upward 45 d compared to mice that receive same dose (12 mg kg-1) of free DTX. The results suggest this synergistic delivery system may be a promising clinical treatment in prostate cancer.
authors.affiliation: Division of Urology, Department of Surgery, Chang Gung Memorial Hospital, Linkou, 5 Fuxing St., Guishan Dist., Taoyuan, 33305, Taiwan.|School of Medicine, Chang Gung University, 259 Wenhua 1st Rd., Guishan Dist., Taoyuan, 33302, Taiwan.
authors.forename: See-Tong
authors.initials: ST
authors.lastname: Pang
authors.affiliation: Institute of Medical Science and Technology, National Sun Yat-sen University, 70 Lienhai Rd., Kaohsiung, 80424, Taiwan.
authors.forename: Feng-Wei
authors.initials: FW
authors.lastname: Lin
authors.affiliation: Division of Urology, Department of Surgery, Chang Gung Memorial Hospital, Linkou, 5 Fuxing St., Guishan Dist., Taoyuan, 33305, Taiwan.|School of Medicine, Chang Gung University, 259 Wenhua 1st Rd., Guishan Dist., Taoyuan, 33302, Taiwan.
authors.forename: Cheng-Keng
authors.initials: CK
authors.lastname: Chuang
authors.affiliation: Institute of Medical Science and Technology, National Sun Yat-sen University, 70 Lienhai Rd., Kaohsiung, 80424, Taiwan.
authors.forename: Hung-Wei
authors.initials: HW
authors.lastname: Yang
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D000077143:Docetaxel; D043425:Glutamate Carboxypeptidase II / Q000276:immunology*; D006801:Humans; D051379:Mice; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics*; D048052:Mitogen-Activated Protein Kinase 3 / Q000235:genetics*; D011094:Polyethyleneimine / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D016019:Survival Analysis; D043823:Taxoids / Q000008:administration & dosage*
chemical_list: D000954:Antigens, Surface; D000972:Antineoplastic Agents, Phytogenic; D034741:RNA, Small Interfering; D043823:Taxoids; D000077143:Docetaxel; D012710:Serum Albumin, Bovine; D011094:Polyethyleneimine; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; C473848:FOLH1 protein, human; D043425:Glutamate Carboxypeptidase II
keywords: docetaxel; layer-by-layer nanoparticles; p44/42 MAPK; prostate cancer; prostate-specific membrane antigen