title: Collapse of chain anadiplosis-structured DNA nanowires for highly sensitive colorimetric assay of nucleic acids.
abstract: In this work, we have proposed a chain anadiplosis-structured DNA nanowire by using two well-defined assembly strands (AS1 and AS2). The presence of a target analyte would drive the single-stranded AS1 dissociate from the pre-formatted nanowire, converting into a fully double-stranded form responsible for extensive accumulation of G-rich cleavage fragment1 (GCF1) because of an autonomously performed polymerization/nicking/displacement process. In turn, the produced GCF1 is able to hybridize with the un-peeled AS2, allowing the replication over AS2 to occur and generate large amounts of G-rich cleavage fragment2 (GCF2) with the ability to hybridize with the un-peeled AS1, thereafter initiating new enzymatic reactions for further collection of GCF1. Because the reactions occur repeatedly, the assembled nanowires gradually dissociated and completely collapsed in the end, achieving the goal of substantial signal amplification for the colorimetric readout of the target analytes. The sensing feasibility is firstly verified by one trigger primer (TP), and then exemplified with the detection of the target, the kras oncogene, with high sensitivity and specificity. As a proof-of-concept strategy, the intelligent signal readout pathway and desired assay ability provide unique insights into the materials research and biological studies.
authors.affiliation: Cancer Metastasis Alert and Prevention Center, and Biopharmaceutical Photocatalysis, State Key Laboratory of Photocatalysis on Energy and Environment, Fuzhou University, Fuzhou 350002, China. pharmlink@gmail.com wuzaisheng@163.com.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Xu
authors.forename: Zai-Sheng
authors.initials: ZS
authors.lastname: Wu
authors.forename: Yanru
authors.initials: Y
authors.lastname: Chen
authors.forename: Tingting
authors.initials: T
authors.lastname: Zheng
authors.forename: Jingqing
authors.initials: J
authors.lastname: Le
authors.forename: Lee
authors.initials: L
authors.lastname: Jia
mesh_terms: D015374:Biosensing Techniques; D003124:Colorimetry; D004247:DNA / Q000737:chemistry*; D006801:Humans; D053770:Nanowires; D021141:Nucleic Acid Amplification Techniques; D009696:Nucleic Acids; D016283:Proto-Oncogene Proteins p21(ras) / Q000032:analysis
chemical_list: C117307:KRAS protein, human; D009696:Nucleic Acids; D004247:DNA; D016283:Proto-Oncogene Proteins p21(ras)