title: Alteration in the ultrastructural morphology of mycelial hyphae and the dynamics of transcriptional activity of lytic enzyme genes during basidiomycete morphogenesis.
abstract: The morphogenesis of macromycetes is a complex multilevel process resulting in a set of molecular-genetic, physiological-biochemical, and morphological-ultrastructural changes in the cells. When the xylotrophic basidiomycetes Lentinus edodes, Grifola frondosa, and Ganoderma lucidum were grown on wood waste as the substrate, the ultrastructural morphology of the mycelial hyphal cell walls differed considerably between mycelium and morphostructures. As the macromycetes passed from vegetative to generative development, the expression of the tyr1, tyr2, chi1, chi2, exg1, exg2, and exg3 genes was activated. These genes encode enzymes such as tyrosinase, chitinase, and glucanase, which play essential roles in cell wall growth and morphogenesis.
authors.affiliation: Laboratory of Microbiology, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences IBPPM RAS, 13 Prospekt Entuziastov, Saratov, 410049, Russian Federation. elenavetrus@yandex.ru.
authors.forename: Elena
authors.initials: E
authors.lastname: Vetchinkina
authors.affiliation: Laboratory of Microbiology, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences IBPPM RAS, 13 Prospekt Entuziastov, Saratov, 410049, Russian Federation.
authors.forename: Maria
authors.initials: M
authors.lastname: Kupryashina
authors.affiliation: Laboratory of Molecular Biology, Kazan Institute of Biochemistry and Biophysics, Kazan Science Center, Russian Academy of Sciences, 2/31 Lobachevsky street, Kazan, 420111, Russian Federation.|Kazan Federal University, 18 Kremlyovskaya street, Kazan, 420008, Russian Federation.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Gorshkov
authors.affiliation: Laboratory of Molecular Biology, Kazan Institute of Biochemistry and Biophysics, Kazan Science Center, Russian Academy of Sciences, 2/31 Lobachevsky street, Kazan, 420111, Russian Federation.
authors.forename: Marina
authors.initials: M
authors.lastname: Ageeva
authors.affiliation: Laboratory of Molecular Biology, Kazan Institute of Biochemistry and Biophysics, Kazan Science Center, Russian Academy of Sciences, 2/31 Lobachevsky street, Kazan, 420111, Russian Federation.|Kazan Federal University, 18 Kremlyovskaya street, Kazan, 420008, Russian Federation.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Gogolev
authors.affiliation: Laboratory of Microbiology, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences IBPPM RAS, 13 Prospekt Entuziastov, Saratov, 410049, Russian Federation.
authors.forename: Valentina
authors.initials: V
authors.lastname: Nikitina
mesh_terms: D015966:Gene Expression Regulation, Fungal; D048691:Grifola / Q000201:enzymology / Q000254:growth & development* / Q000648:ultrastructure; D006867:Hydrolases / Q000096:biosynthesis* / Q000235:genetics; D025301:Hyphae / Q000201:enzymology / Q000254:growth & development* / Q000648:ultrastructure; D008853:Microscopy; D009024:Morphogenesis; D020940:Reishi / Q000201:enzymology / Q000254:growth & development* / Q000648:ultrastructure; D020942:Shiitake Mushrooms / Q000201:enzymology / Q000254:growth & development* / Q000648:ultrastructure; D014158:Transcription, Genetic
chemical_list: D006867:Hydrolases
keywords: basidiomycete morphogenesis; cell wall ultrastructure; gene expression; lytic enzymes; phenol oxidases