title: Differential Routing of Mindbomb1 via Centriolar Satellites Regulates Asymmetric Divisions of Neural Progenitors.
abstract: Unequal centrosome maturation correlates with asymmetric division in multiple cell types. Nevertheless, centrosomal fate determinants have yet to be identified. Here, we show that the Notch pathway regulator Mindbomb1 co-localizes asymmetrically with centriolar satellite proteins PCM1 and AZI1 at the daughter centriole in interphase. Remarkably, while PCM1 and AZI1 remain asymmetric during mitosis, Mindbomb1 is associated with either one or both spindle poles. Asymmetric Mindbomb1 correlates with neurogenic divisions and Mindbomb1 is inherited by the prospective neuron. By contrast, in proliferative divisions, a supplementary pool of Mindbomb1 associated with the Golgi apparatus in interphase is released during mitosis and compensates for Mindbomb1 centrosomal asymmetry. Finally, we show that preventing Mindbomb1 centrosomal association induces reciprocal Notch activation between sister cells and promotes symmetric divisions. Thus, we uncover a link between differential centrosome maturation and Notch signaling and reveal an unexpected compensatory mechanism involving the Golgi apparatus in restoring symmetry in proliferative divisions.
authors.affiliation: Cell Division and Neurogenesis, Institut de Biologie de l'Ecole Normale Supérieure (IBENS), CNRS, Inserm, Ecole Normale Supérieure, PSL Research University, 75005 Paris, France. Electronic address: samuel.tozer@ens.fr.
authors.forename: Samuel
authors.initials: S
authors.lastname: Tozer
authors.affiliation: Cell Division and Neurogenesis, Institut de Biologie de l'Ecole Normale Supérieure (IBENS), CNRS, Inserm, Ecole Normale Supérieure, PSL Research University, 75005 Paris, France; Sorbonne Universités, UPMC Univ Paris 06, IFD, 4 Place Jussieu, 75252 Paris, France.
authors.forename: Chooyoung
authors.initials: C
authors.lastname: Baek
authors.affiliation: Cell Division and Neurogenesis, Institut de Biologie de l'Ecole Normale Supérieure (IBENS), CNRS, Inserm, Ecole Normale Supérieure, PSL Research University, 75005 Paris, France.
authors.forename: Evelyne
authors.initials: E
authors.lastname: Fischer
authors.affiliation: Cell Division and Neurogenesis, Institut de Biologie de l'Ecole Normale Supérieure (IBENS), CNRS, Inserm, Ecole Normale Supérieure, PSL Research University, 75005 Paris, France.
authors.forename: Rosette
authors.initials: R
authors.lastname: Goiame
authors.affiliation: Cell Division and Neurogenesis, Institut de Biologie de l'Ecole Normale Supérieure (IBENS), CNRS, Inserm, Ecole Normale Supérieure, PSL Research University, 75005 Paris, France. Electronic address: xavier.morin@ens.fr.
authors.forename: Xavier
authors.initials: X
authors.lastname: Morin
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002455:Cell Division; D002502:Centrioles / Q000378:metabolism*; D018385:Centrosome / Q000378:metabolism; D002642:Chick Embryo; D006056:Golgi Apparatus / Q000378:metabolism; D008938:Mitosis; D058953:Neural Stem Cells / Q000378:metabolism*; D055495:Neurogenesis; D051880:Receptors, Notch / Q000378:metabolism; D015398:Signal Transduction; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D051880:Receptors, Notch; D044767:Ubiquitin-Protein Ligases
keywords: Golgi apparatus; Mindbomb1; Notch signaling; asymmetric cell division; centriolar satellites; centrosome asymmetry; fate determinants; neural progenitors; neuroepithelium; neurogenesis