title: Hierarchical NiCo₂O₄ Hollow Sphere as a Peroxidase Mimetic for Colorimetric Detection of H₂O₂ and Glucose.
abstract: In this work, the hierarchical NiCo₂O₄ hollow sphere synthesized via a "coordinating etching and precipitating" process was demonstrated to exhibit intrinsic peroxidase-like activity. The peroxidase-like activity of NiCo₂O₄, NiO, and Co₃O₄ hollow spheres were comparatively studied by the catalytic oxidation reaction of 3,3,5,5-tetramethylbenzidine (TMB) in presence of H₂O₂, and a superior peroxidase-like activity of NiCo₂O₄ was confirmed by stronger absorbance at 652 nm. Furthermore, the proposed sensing platform showed commendable response to H₂O₂ with a linear range from 10 μM to 400 μM, and a detection limit of 0.21 μM. Cooperated with GOx, the developed novel colorimetric and visual glucose-sensing platform exhibited high selectivity, favorable reproducibility, satisfactory applicability, wide linear range (from 0.1 mM to 4.5 mM), and a low detection limit of 5.31 μM. In addition, the concentration-dependent color change would offer a better and handier way for detection of H₂O₂ and glucose by naked eye.
authors.affiliation: State Key Laboratory of Marine Resource Utilization in South China Sea, Key Laboratory of Tropical Biological Resources of Ministry of Education Hainan University, College of Material and Chemical Engineering, Haikou 570228, China. hw_hnu@aliyun.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Marine Resource Utilization in South China Sea, Key Laboratory of Tropical Biological Resources of Ministry of Education Hainan University, College of Material and Chemical Engineering, Haikou 570228, China. HDxuchufeng@163.com.
authors.forename: Tianye
authors.initials: T
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Marine Resource Utilization in South China Sea, Key Laboratory of Tropical Biological Resources of Ministry of Education Hainan University, College of Material and Chemical Engineering, Haikou 570228, China. cy507@hainu.edu.cn.
authors.forename: Yang
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Laboratory Cultivation Base of Natural Gas Conversion, School of Chemistry and Chemical Engineering, Ningxia University, Yinchuan 750021, China. xylai@nxu.edu.cn.
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Lai
authors.affiliation: Laboratory Cultivation Base of Natural Gas Conversion, School of Chemistry and Chemical Engineering, Ningxia University, Yinchuan 750021, China. pengjuan@nxu.edu.cn.
authors.forename: Juan
authors.initials: J
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Marine Resource Utilization in South China Sea, Key Laboratory of Tropical Biological Resources of Ministry of Education Hainan University, College of Material and Chemical Engineering, Haikou 570228, China. tujinchun@hainu.edu.cn.
authors.forename: Jinchun
authors.initials: J
authors.lastname: Tu
mesh_terms: D003035:Cobalt; D003124:Colorimetry; D005947:Glucose; D006861:Hydrogen Peroxide; D009532:Nickel; D010100:Oxygen; D009195:Peroxidase; D010544:Peroxidases; D015203:Reproducibility of Results
chemical_list: D003035:Cobalt; D009532:Nickel; D006861:Hydrogen Peroxide; D010544:Peroxidases; D009195:Peroxidase; D005947:Glucose; D010100:Oxygen
keywords: NiCo2O4; colorimetric; hierarchical hollow sphere; peroxidase-like