title: Mometasone Furoate Suppresses PMA-Induced MUC-5AC and MUC-2 Production in Human Airway Epithelial Cells.
abstract: BACKGROUND
  Mucus hypersecretion from airway epithelium is a characteristic feature of airway inflammatory diseases. Tumor necrosis factor α (TNF-α) regulates mucin synthesis. Glucocorticoids including mometasone fuorate (MF) have been used to attenuate airway inflammation. However, effects of MF on mucin production have not been reported.
  
  
  METHODS
  Effects of MF and budesonide (BUD) on the phorbol-12-myristate-13-acetate (PMA)-induction of mucin and TNF-α in human airway epithelial cells (NCI-H292) were investigated in the present study. Confluent NCI-H292 cells were pretreated with PMA (200 nM) for 2 hours. Subsequently, the cells were stimulated with MF (1-500 ng/mL) or BUD (21.5 ng/mL) for 8 hours. Dexamethasone (1 µg/mL) was used as the positive control. Real-time polymerase chain reaction was used to determineMUC2andMUC5ACmRNA levels. The level of total mucin, MUC2, MUC5AC, and TNF-α in culture supernatants were measured using enzyme-linked immunosorbent assay.
  
  
  RESULTS
  MF and BUD significantly suppressedMUC2andMUC5ACgene expression in PMA-stimulated NCI-H292 cells. The inhibitory effects of the two steroid drugs were also observed in the production of total mucin, MUC2 and MUC5AC proteins, and TNF-α.
  
  
  CONCLUSIONS
  Our findings demonstrated that MF and BUD attenuated mucin and TNF-α production in PMA-induced human airway epithelial cells.
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Thammasat University (Rangsit Center), Pathum Thani, Thailand.
authors.forename: Orapan
authors.initials: O
authors.lastname: Poachanukoon
authors.affiliation: Oral Biology Laboratory, Faculty of Dentistry, Thammasat University (Rangsit Center), Pathum Thani, Thailand.
authors.forename: Sittichai
authors.initials: S
authors.lastname: Koontongkaew
authors.affiliation: Oral Biology Laboratory, Faculty of Dentistry, Thammasat University (Rangsit Center), Pathum Thani, Thailand.
authors.forename: Paopanga
authors.initials: P
authors.lastname: Monthanapisut
authors.affiliation: Oral Biology Laboratory, Faculty of Dentistry, Thammasat University (Rangsit Center), Pathum Thani, Thailand.
authors.forename: Napaporn
authors.initials: N
authors.lastname: Pattanacharoenchai
keywords: Budesonide; Epithelial Cells; MUC2 Protein, Human; MUC5AC Protein, Human; Mometasone Fuorate; Mucins; Tumor Necrosis Factor-Alpha