title: One-pot fabrication of FRET-based fluorescent probe for detecting copper ion and sulfide anion in 100% aqueous media.
abstract: The design of effective tools for detecting copper ion (Cu2+) and sulfide anion (S2-) is of great importance due to the abnormal level of Cu2+and S2-has been associated with an increase in risk of many diseases. Herein, we report on the fabrication of fluorescence resonance energy transfer (FRET) based fluorescent probe PF (PEI-FITC) for detecting Cu2+and S2-in 100% aqueous media via a facile one-pot method by covalent linking fluorescein isothiocyanate (FITC) with branched-polyethylenimine (b-PEI). PF could selectively coordinate with Cu2+among 10 metal ions to form PF-Cu2+complex, resulting in fluorescence quenching through FRET mechanism. Furthermore, the in situ generated PF-Cu2+complex can be used to selectively detect S2-based on the displacement approach, resulting in an off-on type sensing. There is no obvious interference from other anions, such as Cl-, NO3-, ClO4-, SO42-, HCO3-, CO32-, Br-, HPO42-, F-and S2O32-. In addition, PF was successfully used to determine Cu2+and S2-in human serum and tap water samples. Therefore, the FRET-based probe PF may provide a new method for selective detection of multifarious analysts in biological and environmental applications, and even hold promise for application in more complicated systems.
authors.affiliation: Key Laboratory of Theoretical Organic Chemistry and Functional Molecule of Ministry of Education, Hunan Provincial Key Laboratory of Controllable Preparation and Functional Application of Fine Polymers, Hunan Province College Key Laboratory of QSAR/QSPR, Institute of Functional Materials, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, Hunan 411201, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Lv
authors.affiliation: Key Laboratory of Theoretical Organic Chemistry and Functional Molecule of Ministry of Education, Hunan Provincial Key Laboratory of Controllable Preparation and Functional Application of Fine Polymers, Hunan Province College Key Laboratory of QSAR/QSPR, Institute of Functional Materials, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, Hunan 411201, China. Electronic address: cj0066@gmail.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Laboratory of Theoretical Organic Chemistry and Functional Molecule of Ministry of Education, Hunan Provincial Key Laboratory of Controllable Preparation and Functional Application of Fine Polymers, Hunan Province College Key Laboratory of QSAR/QSPR, Institute of Functional Materials, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, Hunan 411201, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Theoretical Organic Chemistry and Functional Molecule of Ministry of Education, Hunan Provincial Key Laboratory of Controllable Preparation and Functional Application of Fine Polymers, Hunan Province College Key Laboratory of QSAR/QSPR, Institute of Functional Materials, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, Hunan 411201, China. Electronic address: pshzhang07@gmail.com.
authors.forename: Peisheng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Theoretical Organic Chemistry and Functional Molecule of Ministry of Education, Hunan Provincial Key Laboratory of Controllable Preparation and Functional Application of Fine Polymers, Hunan Province College Key Laboratory of QSAR/QSPR, Institute of Functional Materials, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, Hunan 411201, China.
authors.forename: Maolin
authors.initials: M
authors.lastname: Yu
authors.affiliation: Key Laboratory of Theoretical Organic Chemistry and Functional Molecule of Ministry of Education, Hunan Provincial Key Laboratory of Controllable Preparation and Functional Application of Fine Polymers, Hunan Province College Key Laboratory of QSAR/QSPR, Institute of Functional Materials, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, Hunan 411201, China.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Long
authors.affiliation: Key Laboratory of Theoretical Organic Chemistry and Functional Molecule of Ministry of Education, Hunan Provincial Key Laboratory of Controllable Preparation and Functional Application of Fine Polymers, Hunan Province College Key Laboratory of QSAR/QSPR, Institute of Functional Materials, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, Hunan 411201, China.
authors.forename: Pinggui
authors.initials: P
authors.lastname: Yi
mesh_terms: D000838:Anions; D003300:Copper / Q000032:analysis* / Q000097:blood; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013440:Sulfides / Q000032:analysis* / Q000097:blood; D014867:Water / Q000737:chemistry*
chemical_list: D000838:Anions; D005456:Fluorescent Dyes; D013440:Sulfides; D014867:Water; D003300:Copper
keywords: Copper ion; FITC; FRET; Fluorescence quenching; Sulfide anion