title: A hybrid monolithic column based on boronate-functionalized graphene oxide nanosheets for online specific enrichment of glycoproteins.
abstract: A hybrid monolithic column based on aminophenylboronic acid (APBA)-functionalized graphene oxide (GO) has been developed and used for selective enrichment of glycoproteins. The APBA/GO composites were homogeneously incorporated into a polymer monolithic column with the help of oligomer matrix and followed by in situ polymerization. The effect of dispersion of APBA/GO composites in the polymerization mixture on the performance of the monolithic column was explored in detail. The presence of graphene oxide not only enlarged the BET surface area from 6.3m2/g to 169.4m2/g, but also provided abundant boronic acid moieties for glycoprotein extraction, which improved the enrichment selectivity and efficiency for glycoproteins. The APBA/GO hybrid monolithic column was incorporated into a sequential injection system, which facilitated online extraction of proteins. Combining the superior properties of extraordinary surface area of GO and the affinity interaction of APBA to glycoproteins, the APBA/GO hybrid monolithic column showed higher enrichment factors for glycoproteins than other proteins without cis-diol-containing groups. Also, under comparable or even shorter processing time and without the addition of any organic solvent, it showed higher binding capacity toward glycoproteins compared with the conventional boronate affinity monolithic column. The practical applicability of this system was demonstrated by processing of egg white samples for extraction of ovalbumin and ovotransferrin, and satisfactory results were obtained by assay with SDS-PAGE.
authors.affiliation: School of Chemistry, Sun Yat-sen University, Guangzhou, 510275, China.
authors.forename: Chanyuan
authors.initials: C
authors.lastname: Zhou
authors.affiliation: School of Chemistry, Sun Yat-sen University, Guangzhou, 510275, China.
authors.forename: Xiaoman
authors.initials: X
authors.lastname: Chen
authors.affiliation: School of Chemistry, Sun Yat-sen University, Guangzhou, 510275, China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Du
authors.affiliation: School of Chemistry, Sun Yat-sen University, Guangzhou, 510275, China. Electronic address: cesgkl@mail.sysu.edu.cn.
authors.forename: Gongke
authors.initials: G
authors.lastname: Li
authors.affiliation: School of Chemistry, Sun Yat-sen University, Guangzhou, 510275, China. Electronic address: xiaoxhua@mail.sysu.edu.cn.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
authors.forename: Zongwei
authors.initials: Z
authors.lastname: Cai
mesh_terms: D001897:Boronic Acids / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification*; D006108:Graphite / Q000737:chemistry*; D009930:Organic Chemicals / Q000737:chemistry; D010087:Oxides / Q000737:chemistry*; D058105:Polymerization; D011108:Polymers; D012997:Solvents / Q000737:chemistry
chemical_list: D001897:Boronic Acids; D006023:Glycoproteins; D009930:Organic Chemicals; D010087:Oxides; D011108:Polymers; D012997:Solvents; C028592:3-aminobenzeneboronic acid; D006108:Graphite
keywords: Boronate affinity; Glycoproteins; Graphene oxide nanosheets; Hybrid monolithic column; On-line enrichment