title: TNF-α-inducing protein of Helicobacter pylori induces epithelial-mesenchymal transition (EMT) in gastric cancer cells through activation of IL-6/STAT3 signaling pathway.
abstract: Tumor necrosis factor (TNF)-α-inducing protein (Tipα) is a newly identified carcinogenic factor secreted by Helicobacter pylori (H. pylori). Although it has been proved that Tipα is a strong inducer of epithelial-mesenchymal transition (EMT), a crucial process of migration, the exact molecular mechanism is unknown. Current evidence indicates that the oncogenic transcription factor signal transducers and activators of transcription 3 (STAT3) is inappropriately activated in multiple malignancies, including gastric cancer. In this study, we showed that Tipα significantly down-regulated the expression of EMT-related markers E-cadherin as well as up-regulated N-cadherin and vimentin in SGC7901 cells, with typical morphological changes of EMT. Tipα also promoted proliferation and migration of SGC7901 cells. Furthermore, Tipα activated interleukin-6 (IL-6)/STAT3 signaling pathway in SGC7901 cells. The effects of Tipα treatment observed was abolished when we block IL-6/STAT3 signaling pathway. Altogether, our data demonstrated that Tipα may accelerate tumor aggressiveness in gastric cancer by promoting EMT through activation of IL-6/STAT3 pathway.
authors.affiliation: Institute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, PR China. Electronic address: 815003608@qq.com.
authors.forename: Guodong
authors.initials: G
authors.lastname: Chen
authors.affiliation: Institute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, PR China. Electronic address: 374069569@qq.com.
authors.forename: Na
authors.initials: N
authors.lastname: Tang
authors.affiliation: Institute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, PR China. Electronic address: 1019338557@qq.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Institute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, PR China. Electronic address: 947761272@qq.com.
authors.forename: Linqiao
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Institute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, PR China. Electronic address: 52813707@qq.com.
authors.forename: Minjun
authors.initials: M
authors.lastname: Yu
authors.affiliation: Institute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, PR China. Electronic address: zhaolanhua@126.com.
authors.forename: Lanhua
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Institute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, PR China. Electronic address: 343968162@qq.com.
authors.forename: Hengling
authors.initials: H
authors.lastname: Cai
authors.affiliation: Institute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, PR China. Electronic address: hanliang0122@vip.qq.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Han
authors.affiliation: Institute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, PR China. Electronic address: 3844843762@qq.com.
authors.forename: Chengyuan
authors.initials: C
authors.lastname: Xie
authors.affiliation: Institute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, PR China. Electronic address: nhzhangyan@126.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D001426:Bacterial Proteins / Q000502:physiology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*
chemical_list: D001426:Bacterial Proteins; D015850:Interleukin-6; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; C505332:Tipalpha protein, Helicobacter pylori
keywords: Epithelial-mesenchymal transition (EMT); Gastric cancer; Helicobacter pylori; IL-6/STAT3 pathway; TNF-α inducing protein (Tipα)