title: Optogenetic inhibition of behavior with anion channelrhodopsins.
abstract: Optogenetics uses light exposure to manipulate physiology in genetically modified organisms. Abundant tools for optogenetic excitation are available, but the limitations of current optogenetic inhibitors present an obstacle to demonstrating the necessity of neuronal circuits. Here we show that anion channelrhodopsins can be used to specifically and rapidly inhibit neural systems involved in Drosophila locomotion, wing expansion, memory retrieval and gustation, thus demonstrating their broad utility in the circuit analysis of behavior.
authors.affiliation: Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School, Singapore.
authors.forename: Farhan
authors.initials: F
authors.lastname: Mohammad
authors.affiliation: Institute for Molecular and Cell Biology, Agency for Science Technology and Research, Singapore.
authors.forename: James C
authors.initials: JC
authors.lastname: Stewart
authors.affiliation: Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School, Singapore.
authors.forename: Stanislav
authors.initials: S
authors.lastname: Ott
authors.affiliation: Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School, Singapore.
authors.forename: Katarina
authors.initials: K
authors.lastname: Chlebikova
authors.affiliation: Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School, Singapore.
authors.forename: Jia Yi
authors.initials: JY
authors.lastname: Chua
authors.affiliation: Temasek Life Sciences Laboratory, Singapore.
authors.forename: Tong-Wey
authors.initials: TW
authors.lastname: Koh
authors.affiliation: Institute for Molecular and Cell Biology, Agency for Science Technology and Research, Singapore.
authors.forename: Joses
authors.identifier: 0000-0002-9186-6322
authors.initials: J
authors.lastname: Ho
authors.affiliation: Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School, Singapore.|Institute for Molecular and Cell Biology, Agency for Science Technology and Research, Singapore.|Department of Physiology, National University of Singapore, Singapore.
authors.forename: Adam
authors.initials: A
authors.lastname: Claridge-Chang
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects* / Q000502:physiology; D004330:Drosophila / Q000502:physiology*; D008027:Light; D008124:Locomotion / Q000502:physiology; D009434:Neural Pathways / Q000502:physiology*; D009474:Neurons / Q000502:physiology; D062308:Optogenetics / Q000379:methods*; D030781:Organisms, Genetically Modified; D012243:Rhodopsin / Q000494:pharmacology*; D055697:Taste Perception / Q000502:physiology; D050994:Voltage-Dependent Anion Channels / Q000502:physiology
chemical_list: D050994:Voltage-Dependent Anion Channels; D012243:Rhodopsin