title: Identification, ontogeny and expression analysis of a novel laboratory of genetics and physiology 2 (LGP2) transcript in Asian seabass, Lates calcarifer.
abstract: LGP2 (laboratory of genetics and physiology 2) is an important member of the retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs), which plays a significant role in antiviral innate immunity. In this study, we have cloned the full-length cDNA sequence of LGP2 from Asian seabass, Lates calcarifer (AsLGP2). The complete AsLGP2 cDNA sequence consisted of 2586 nucleotides encoding a putative protein of 681 amino acids with a molecular mass of 77.6 kDa. From the AsLGP2 protein, four different conserved domains were predicted: a DExDc (DEAD/DEAH box helicase domain), a bacterial type III restriction enzyme domain (RES III), a HELICc (Helicase superfamily c-terminal domain and a RIG-I_C-RD (RIG-I C-terminal regulatory domain). The transcript of AsLGP2 could be detected in all the 11 tissues tested in healthy animals with high expression noticed in tissues facing external environment such as gill, hindgut and skin. The ontogenic expression profile of AsLGP2 implies a possible maternal transfer of this gene as it has been detected in all early embryonic developmental stages along with unfertilized eggs. Viral analogue, poly I:C, injection resulted in rapid up-regulated expression in different tissues with the highest modulation of expression observed in kidney followed by liver and gill. A rapid response of AsLGP2 expression was also observed in the different tissues of Vibrio alginolyticus-injected L. calcarifer, while significant change in expression was noticed following Staphylococcus aureus infection. Similarly, exposure to different pathogen-mimicking microbial analogues such as poly I:C, LPS and PGN resulted in enhanced expression of AsLGP2 in SISK cell-line. Taking together, these observations suggest that AsLGP2 can act as both antiviral and antibacterial cytosolic receptor and may play a significant role in embryonic and larval development in marine euryhaline teleosts like Asian seabass.
authors.affiliation: ICAR-Central Institute of Fisheries Education (CIFE), Off-Yari Road, Versova, Mumbai, 400 061, India.
authors.forename: Anutosh
authors.initials: A
authors.lastname: Paria
authors.affiliation: ICAR-Central Institute of Fisheries Education (CIFE), Off-Yari Road, Versova, Mumbai, 400 061, India.
authors.forename: A
authors.initials: A
authors.lastname: Deepika
authors.affiliation: ICAR-Central Institute of Fisheries Education (CIFE), Off-Yari Road, Versova, Mumbai, 400 061, India.
authors.forename: K
authors.initials: K
authors.lastname: Sreedharan
authors.affiliation: ICAR-Central Institute of Fisheries Education (CIFE), Off-Yari Road, Versova, Mumbai, 400 061, India.
authors.forename: M
authors.initials: M
authors.lastname: Makesh
authors.affiliation: ICAR-Central Institute of Fisheries Education (CIFE), Off-Yari Road, Versova, Mumbai, 400 061, India.
authors.forename: Aparna
authors.initials: A
authors.lastname: Chaudhari
authors.affiliation: ICAR-Central Institute of Fisheries Education (CIFE), Off-Yari Road, Versova, Mumbai, 400 061, India.
authors.forename: C S
authors.initials: CS
authors.lastname: Purushothaman
authors.affiliation: ICAR-Central Institute of Fisheries Education (CIFE), Off-Yari Road, Versova, Mumbai, 400 061, India. Electronic address: rajendrankv@hotmail.com.
authors.forename: K V
authors.initials: KV
authors.lastname: Rajendran
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005393:Fish Diseases / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000069452:Pathogen-Associated Molecular Pattern Molecules; D010473:Perciformes; D010802:Phylogeny; D020365:RNA Helicases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment / Q000662:veterinary; D013203:Staphylococcal Infections / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus; D014018:Tissue Distribution; D014735:Vibrio Infections / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D044149:Vibrio alginolyticus
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D000069452:Pathogen-Associated Molecular Pattern Molecules; D012333:RNA, Messenger; D020365:RNA Helicases
keywords: Asian seabass; Bacterial infection; Gene expression; LGP2; Laboratory of genetics and physiology 2; Lates calcarifer; Ontogeny; PRR; Poly I:C; RLR