title: Proteome Changes of Human Bone Marrow Mesenchymal Stem Cells Induced by 1,4-Benzoquinone.
abstract: Benzene is metabolized to hydroquinone in liver and subsequently transported to bone marrow for further oxidization to 1,4-benzoquinone (1,4-BQ), which may be related to the leukemia and other blood disorders. In the present study, we investigated the proteome profiles of human primary bone marrow mesenchymal stem cells (hBM-MSCs) treated by 1,4-BQ. We identified 32 proteins that were differentially expressed. Two of them, HSP27 and Vimentin, were verified at both mRNA and protein levels and their cellular localization was examined by immunofluorescence. We also found increased mRNA level of RAP1GDS1, a critical factor of metabolism that has been identified as a fusion partner in various hematopoietic malignancies. Therefore, these differentially expressed proteins can play important roles in benzene-mediated hematoxicity.
authors.affiliation: Institute of Occupational Medicine, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, China.
authors.forename: Wei
authors.identifier: 0000-0002-4091-155X
authors.initials: W
authors.lastname: Wu
authors.affiliation: Reproduction Medicine Center, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, China.
authors.forename: Ling
authors.identifier: 0000-0002-7917-7671
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Institute of Occupational Medicine, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, China.
authors.forename: Min
authors.identifier: 0000-0002-4399-0425
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Institute of Occupational Medicine, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, China.
authors.forename: Li-Jin
authors.identifier: 0000-0002-8206-0570
authors.initials: LJ
authors.lastname: Zhu
authors.affiliation: Institute of Occupational Medicine, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, China.
authors.forename: Hai-Ling
authors.identifier: 0000-0003-1385-442X
authors.initials: HL
authors.lastname: Xia
authors.affiliation: Institute of Occupational Medicine, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, China.
authors.forename: Jian-Lin
authors.identifier: 0000-0002-4052-7865
authors.initials: JL
authors.lastname: Lou
authors.affiliation: Department of Diagnosis, The 4th Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150001, China.
authors.forename: Jia-Ren
authors.identifier: 0000-0001-6653-0053
authors.initials: JR
authors.lastname: Liu
authors.affiliation: Institute of Occupational Medicine, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, China; Department of Environmental Medicine, School of Public Health, Zhejiang University, Hangzhou, Zhejiang 310058, China.
authors.forename: Yun
authors.identifier: 0000-0002-4736-3883
authors.initials: Y
authors.lastname: Xiao
mesh_terms: D000328:Adult; D016227:Benzoquinones / Q000494:pharmacology*; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000378:metabolism*; D002478:Cells, Cultured; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D055815:Young Adult
chemical_list: D016227:Benzoquinones; D020543:Proteome; C004532:quinone