title: Organization of glomerular territories in the olfactory bulb of post-embryonic wild chinook salmon Oncorhynchus tshawytscha.
abstract: The post-embryonic odor imprinting paradigm suggests Chinook salmon (Oncorhynchus tshawytscha) acquire memory to stream-specific amino acid olfactory odors prior to emergence as fry. Because effects of olfactory experience on development can be examined by mapping olfactory sensory neurons extending into distinct territories of glomerular neuropil in the olfactory bulb, glomerular patterning from early yolk-sac larva to fry was documented in wild salmonids, a temporal scale not yet thoroughly explored. Labeling olfactory sensory neurons with anti-keyhole limpet hemocyanin (anti-KLH) revealed seven spatially conserved glomerular territories visible at hatch and well established by the late yolk-sac larva developmental stage. Because of the responsiveness of microvillous olfactory sensory neurons to amino acids, corresponding glomeruli in the lateral bulbar region were mapped using anti-calretinin. The dorsolateral territory, distinct glomeruli of the lateral glomerular territory and the ventromedial glomeruli were immunoreactive to both KLH and calretinin. This study offers a morphological description of glomerular patterning in post-embryonic stages in wild Chinook salmon, a temporal window previously shown to be significant for olfactory imprinting. J. Morphol. 278:464-474, 2017. © 2017 Wiley Periodicals, Inc.
authors.affiliation: Department of Biological Sciences, University of Windsor, 401 Sunset Avenue, Windsor, Ontario, Canada, N9B 3P4.
authors.forename: Cory L
authors.initials: CL
authors.lastname: Ochs
authors.affiliation: Department of Biological Sciences, University of Windsor, 401 Sunset Avenue, Windsor, Ontario, Canada, N9B 3P4.
authors.forename: Tina
authors.initials: T
authors.lastname: Suntres
authors.affiliation: Department of Biological Sciences, University of Windsor, 401 Sunset Avenue, Windsor, Ontario, Canada, N9B 3P4.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Zygowska
authors.affiliation: Department of Biological Sciences, University of Windsor, 401 Sunset Avenue, Windsor, Ontario, Canada, N9B 3P4.|Great Lakes Institute for Environmental Research, University of Windsor, 2990 Riverside Dr. W. Windsor, Ontario, N9C 1A2.
authors.forename: Trevor
authors.initials: T
authors.lastname: Pitcher
authors.affiliation: Department of Biological Sciences, University of Windsor, 401 Sunset Avenue, Windsor, Ontario, Canada, N9B 3P4.|Great Lakes Institute for Environmental Research, University of Windsor, 2990 Riverside Dr. W. Windsor, Ontario, N9C 1A2.
authors.forename: Barbara S
authors.initials: BS
authors.lastname: Zielinski
mesh_terms: D000818:Animals; D064032:Calbindin 2 / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006433:Hemocyanins / Q000276:immunology; D007814:Larva / Q000378:metabolism; D009830:Olfactory Bulb / Q000033:anatomy & histology* / Q000254:growth & development*; D012474:Salmon / Q000033:anatomy & histology* / Q000254:growth & development*; D015017:Yolk Sac / Q000378:metabolism
chemical_list: D064032:Calbindin 2; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin
keywords: Chinook salmon; alevin; calretinin; glomeruli; keyhole limpet hemocyanin; olfaction