title: Design of a portable imager for near-infrared visualization of cutaneous wounds.
abstract: A portable imager developed for real-time imaging of cutaneous wounds in research settings is described. The imager consists of a high-resolution near-infrared CCD camera capable of detecting both bioluminescence and fluorescence illuminated by an LED ring with a rotatable filter wheel. All external components are integrated into a compact camera attachment. The device is demonstrated to have competitive performance with a commercial animal imaging enclosure box setup in beam uniformity and sensitivity. Specifically, the device was used to visualize the bioluminescence associated with increased reactive oxygen species activity during the wound healing process in a cutaneous wound inflammation model. In addition, this device was employed to observe the fluorescence associated with the activity of matrix metalloproteinases in a mouse lipopolysaccharide-induced infection model. Our results support the use of the portable imager design as a noninvasive and real-time imaging tool to assess the extent of wound inflammation and infection.
authors.affiliation: University of Texas at Arlington, Department of Electrical Engineering, P.O. Box 19016, Arlington, Texas 76019, United States.
authors.forename: Zhaoqiang
authors.initials: Z
authors.lastname: Peng
authors.affiliation: University of Texas at Arlington, Department of Bioengineering, P.O. Box 19138, Arlington, Texas 76019, United States.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.affiliation: University of Texas at Arlington, Department of Bioengineering, P.O. Box 19138, Arlington, Texas 76019, United States.
authors.forename: Ashley
authors.initials: A
authors.lastname: Dacy
authors.affiliation: University of Texas at Arlington, Department of Electrical Engineering, P.O. Box 19016, Arlington, Texas 76019, United States.
authors.forename: Deyin
authors.initials: D
authors.lastname: Zhao
authors.affiliation: University of Texas at Arlington, Department of Bioengineering, P.O. Box 19138, Arlington, Texas 76019, United States.
authors.forename: Vasant
authors.initials: V
authors.lastname: Kearney
authors.affiliation: University of Texas at Arlington, Department of Electrical Engineering, P.O. Box 19016, Arlington, Texas 76019, United States.
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhou
authors.affiliation: University of Texas at Arlington, Department of Bioengineering, P.O. Box 19138, Arlington, Texas 76019, United States.
authors.forename: Liping
authors.initials: L
authors.lastname: Tang
authors.affiliation: Progenitec Inc., 917 Parktree Drive, Arlington, Texas 76015, United States.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Hu
mesh_terms: D000818:Animals; D005453:Fluorescence; D061848:Optical Imaging / Q000295:instrumentation*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012867:Skin / Q000293:injuries*; D019265:Spectroscopy, Near-Infrared / Q000295:instrumentation; D014945:Wound Healing; D014947:Wounds and Injuries / Q000000981:diagnostic imaging / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species