title: Periodic elevation of regulatory T cells on the day of embryo transfer is associated with better in vitro fertilization outcome.
abstract: Treg cells have been shown to be important in maintaining maternofetal tolerance, but the expression of Tregs in assisted reproductive technology (ART) in women on the day of embryo transfer (D0), 5days (D5) and 14days after ET (D14); the related factors influencing the expression levels of Tregs; the proliferation ability and the relevant cytokine epression by Tregs on D14 have not been investigated. In this study, 124 women undergoing in vitro fertilization-intracytoplasmic sperm injection (IVF/ICSI) were enrolled. Early morning fasting blood samples were obtained for the measurement of Tregs and other relevant indicators on the D0, D5and D14days after ET. we showed that the Tregs were increased on D0 and D14 in pregnant women, while there was no obvious fluctuation in non-pregnant women. IL-10 and TGF-β levels and the expansion of Tregs were significantly higher in successfully pregnant women than in non-pregnant women on D14. The levels of E2, P did not significantly differ between the groups. We suggest that periodic elevation of Tregs on the day of ET was associated with higher embryo implantation rate after ART.
authors.affiliation: Reproduction Medical Center, Yantai Yuhuangding Hospital of Qingdao University, 20 Yuhuangding East Road, Yantai 264000, PR China. Electronic address: sdwangwj@126.com.
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Wang
authors.affiliation: Central Laboratory, Yantai Yuhuangding Hospital of Qingdao University, 20 Yuhuangding East Road, Yantai 264000, PR China.
authors.forename: Fu-Jun
authors.initials: FJ
authors.lastname: Liu
authors.affiliation: Clinical Laboratory, Yantai Yuhuangding Hospital of Qingdao University, Yantai 264000, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Reproduction Medical Center, Yantai Yuhuangding Hospital of Qingdao University, 20 Yuhuangding East Road, Yantai 264000, PR China.
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Liu
authors.affiliation: Reproduction Medical Center, Yantai Yuhuangding Hospital of Qingdao University, 20 Yuhuangding East Road, Yantai 264000, PR China.
authors.forename: Qing-Lan
authors.initials: QL
authors.lastname: Qu
authors.affiliation: Reproduction Medical Center, Yantai Yuhuangding Hospital of Qingdao University, 20 Yuhuangding East Road, Yantai 264000, PR China.
authors.forename: Feng-Hua
authors.initials: FH
authors.lastname: Li
authors.affiliation: Reproduction Medical Center, Yantai Yuhuangding Hospital of Qingdao University, 20 Yuhuangding East Road, Yantai 264000, PR China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Zhuang
authors.affiliation: Reproduction Medical Center, Yantai Yuhuangding Hospital of Qingdao University, 20 Yuhuangding East Road, Yantai 264000, PR China.
authors.forename: Xiao-Xiao
authors.initials: XX
authors.lastname: Li
authors.affiliation: Reproduction Medical Center, Yantai Yuhuangding Hospital of Qingdao University, 20 Yuhuangding East Road, Yantai 264000, PR China.
authors.forename: Cui-Fang
authors.initials: CF
authors.lastname: Hao
mesh_terms: D000328:Adult; D049109:Cell Proliferation; D002478:Cells, Cultured; D010064:Embryo Implantation; D005260:Female; D005307:Fertilization in Vitro; D051858:Forkhead Transcription Factors / Q000378:metabolism; D012739:Gonadal Steroid Hormones / Q000378:metabolism; D006801:Humans; D007108:Immune Tolerance; D016753:Interleukin-10 / Q000378:metabolism; D011247:Pregnancy; D018873:Pregnancy Rate; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D012739:Gonadal Steroid Hormones; D016212:Transforming Growth Factor beta; D016753:Interleukin-10
keywords: ART; E2; IL-10; Sex hormone; TGF-β; Tregs