title: EFFECT OF INDOLE-3-ACETIC ACID, KINETIN, AND ETHYLENEDIAMINETETRAACETIC ACID ON PLANT GROWTH AND UPTAKE AND TRANSLOCATION OF LEAD, MICRONUTRIENTS, AND MACRONUTRIENTS IN ALFALFA PLANTS.
abstract: Alfalfa plants germinated and grown for 15 d in soil containing 80 mg Pb kg-1were treated with ethylenediaminetetraacetic acid (EDTA) at 0.8 mM and indole-3-acetic acid-kinetin (IAA-KN) at 100 μM. Fifteen days after the treatment application, the concentration of lead (Pb), macronutrients, and micronutrients was determined using inductively coupled plasma/optical emission spectroscopy. The chlorophyll content and plant growth were also measured. Roots of plants exposed to Pb alone, Pb-EDTA, and Pb-EDTA-IAA-KN had 160, 140, and 150 mg Pb kg-1DW, respectively. Pb was not detected in the stems of plants exposed to Pb alone; however, stems of plants treated with EDTA and EDTA-IAA-KN had 78 and 142 mg Pb kg-1DW, respectively. While the Pb concentration in leaves of plants treated with EDTA and EDTA-IAA-KN was 92 and 127 mg kg-1DW, respectively. In addition, EDTA and EDTA-IAA-KN significantly increased the translocation of zinc and manganese to leaves. The x-ray absorption spectroscopic studies demonstrated that Pb(II) was transported from roots to leaves without a change in the oxidation state.
authors.affiliation: a Chemistry Department , The University of Texas at El Paso , El Paso , Texas , USA.
authors.forename: Martha Laura
authors.initials: ML
authors.lastname: López
authors.affiliation: a Chemistry Department , The University of Texas at El Paso , El Paso , Texas , USA.
authors.forename: José R
authors.initials: JR
authors.lastname: Peralta-Videa
authors.affiliation: a Chemistry Department , The University of Texas at El Paso , El Paso , Texas , USA.
authors.forename: Jason G
authors.initials: JG
authors.lastname: Parsons
authors.affiliation: b Chemistry Department and Environmental Sciences and Engineering Ph.D. Program , The University of Texas at El Paso , El Paso , Texas , USA.
authors.forename: Jorge L
authors.initials: JL
authors.lastname: Gardea-Torresdey
authors.affiliation: c Department of Health Promotion, College of Health Sciences , The University of Texas at El Paso , El Paso , Texas , USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Duarte-Gardea
mesh_terms: D001673:Biodegradation, Environmental; D004492:Edetic Acid; D007210:Indoleacetic Acids; D007701:Kinetin; D007854:Lead / Q000302:isolation & purification / Q000378:metabolism*; D000455:Medicago sativa / Q000378:metabolism*; D018977:Micronutrients; D063245:Plant Development; D018515:Plant Leaves / Q000254:growth & development; D018517:Plant Roots / Q000254:growth & development; D012989:Soil Pollutants / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D007210:Indoleacetic Acids; D018977:Micronutrients; D012989:Soil Pollutants; D007854:Lead; C030737:indoleacetic acid; D004492:Edetic Acid; D007701:Kinetin
keywords: ethylenediaminetetraacetic acid (EDTA); indole-3-acetic acid; kinetin; lead (Pb); macronutrients; micronutrients; uptake