title: Non-target analysis of phospholipid and sphingolipid species in egg yolk using liquid chromatography/triple quadrupole tandem mass spectrometry.
abstract: In this work, phospholipids extracted from egg yolk (control group, experimental group) were identified using high performance liquid chromatography coupled with electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS). Combinations of fatty acyls occurring in 11 classes of phospholipids from egg yolk were investigated. Differences between the profile of fatty acyls from hens fed traditionally and the ones that received special diet supplementation were observed. Experimental findings were complemented with multivariate chemometric analysis. Multiple reaction monitoring mass spectrometry mode was utilized and 123 distinct combinations of fatty acyls occurring in phospholipids were identified. From these, large portions are polyunsaturated fatty acyls from the omega-3 and omega-6 family. HPLC MS/MS analysis allows for quick, accurate and precise determination of biologically active compounds, found in low concentrations within the tested material.
authors.affiliation: Department of Environmental Chemistry and Bioanalytics Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100 Torun, Poland; Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Wileńska 4, 87-100 Toruń, Poland. Electronic address: bbusz@chem.umk.pl.
authors.forename: Bogusław
authors.initials: B
authors.lastname: Buszewski
authors.affiliation: Department of Environmental Chemistry and Bioanalytics Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100 Torun, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Walczak
authors.affiliation: Department of Chemical Engineering, Pukyong National University, 365 Sinseon-ro, 48-547, Busan, South Korea.
authors.forename: Petar
authors.initials: P
authors.lastname: Žuvela
authors.affiliation: Department of Chemical Engineering, Pukyong National University, 365 Sinseon-ro, 48-547, Busan, South Korea.
authors.forename: J Jay
authors.initials: JJ
authors.lastname: Liu
mesh_terms: D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D004530:Egg Yolk / Q000737:chemistry*; D015525:Fatty Acids, Omega-3 / Q000032:analysis; D043371:Fatty Acids, Omega-6 / Q000032:analysis; D005260:Female; D010743:Phospholipids / Q000032:analysis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013107:Sphingolipids / Q000032:analysis*; D053719:Tandem Mass Spectrometry
chemical_list: D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D010743:Phospholipids; D013107:Sphingolipids
keywords: Egg yolk; Fatty acyls; HPLC–MS/MS; MRM; Phospholipids