title: Boronic Acid vs. Folic Acid: A Comparison of the bio-recognition performances by Impedimetric Cytosensors based on Ferrocene cored dendrimer.
abstract: A comparative study is reported where folic acid (FA) and boronic acid (BA) based cytosensors and their analytical performances in cancer cell detection were analyzed by using electrochemical impedance spectroscopy (EIS) method. Cytosensors were fabricated using self-assembled monolayer principle by modifying Au electrode with cysteamine (Cys) and immobilization of ferrocene cored polyamidiamine dendrimers second generation (Fc-PAMAM (G2)), after which electrodes were modified with FA and BA. Au/Fc-PAMAM(G2)/FA and Au/Fc-PAMAM(G2)/BA based cytosensors showed extremely good analytical performances in cancer cell detection with linear range of 1×102to 1×106cellsml-1, detection limit of 20cellsml-1with incubation time of 20min for FA based electrode, and for BA based electrode detection limit was 28cellsml-1with incubation time of 10min. Next to excellent analytical performances, cytosensors showed high selectivity towards cancer cells which was demonstrated in selectivity study using human embryonic kidney 293 cells (HEK 293) as normal cells and Au/Fc-PAMAM(G2)/FA electrode showed two times better selectivity than BA modified electrode. These cytosensors are promising for future applications in cancer cell diagnosis.
authors.affiliation: Biotechnology Research Lab, EMC Technology Inc, ARGEM Building, Technocity, Avcılar, Istanbul 34320, Turkey.
authors.forename: Muamer
authors.initials: M
authors.lastname: Dervisevic
authors.affiliation: Biotechnology Research Lab, EMC Technology Inc, ARGEM Building, Technocity, Avcılar, Istanbul 34320, Turkey. Electronic address: msenel81@gmail.com.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Şenel
authors.affiliation: Orta Mah. Sunay Sok. No 1, Kartal, Istanbul 34880, Turkey.
authors.forename: Tugba
authors.initials: T
authors.lastname: Sagir
authors.affiliation: Biotechnology Research Lab, EMC Technology Inc, ARGEM Building, Technocity, Avcılar, Istanbul 34320, Turkey.
authors.forename: Sevim
authors.initials: S
authors.lastname: Isik
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D045744:Cell Line, Tumor; D050091:Dendrimers / Q000737:chemistry*; D058266:Dielectric Spectroscopy / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D004867:Equipment Design; D005296:Ferrous Compounds / Q000737:chemistry*; D005492:Folic Acid / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D057809:HEK293 Cells; D006801:Humans; D000075163:Metallocenes; D009369:Neoplasms / Q000175:diagnosis*; D015203:Reproducibility of Results
chemical_list: D050091:Dendrimers; D005296:Ferrous Compounds; D000075163:Metallocenes; C104700:PAMAM Starburst; D006046:Gold; D005492:Folic Acid; C004998:ferrocene
keywords: Boronic acid; Cancer cells; Cytosensor; Electrochemical impedance; Ferrocene; Folic acid