title: Homo-aro-cholestane, furostane and spirostane saponins from the tubers of Ophiopogon japonicus.
abstract: Phytochemical investigation of the tubers of Ophiopogon japonicus led to the isolation of five previously undescribed steroidal saponins, ophiojaponins A-E, together with twelve known ones. The structures of these isolated compounds were elucidated by detailed spectroscopic analyses and chemical methods. Ophiojaponins A-C are rare naturally occurring C29steroidal glycosides possessing a homo-cholestane skeleton with an aromatized ring E. Ruscogenin 1-O-α-L-rhamnopyranosyl-(1 → 2)-4-O-sulfo-β-D-fucopyranosido-3-O-β-D-glucopyranoside was isolated as single component and its full spectroscopic data was reported for the first time. The isolated steroidal saponins were evaluated for their cytotoxicities against two human tumor cell lines MG-63 and SNU387. Among them, five known spirostane-type glycosides showed cytotoxic activity against both MG-63 and SNU387 cell lines with IC50values ranging from 0.76 to 27.0 μM.
authors.affiliation: School of Pharmacy and Bioengineering, Chengdu University, Chengdu, 610106, China; Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.
authors.forename: Lun
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.
authors.forename: Xi-Lan
authors.initials: XL
authors.lastname: Jiang
authors.affiliation: School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610013, China.
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Zhang
authors.affiliation: School of Pharmacy and Bioengineering, Chengdu University, Chengdu, 610106, China; Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China. Electronic address: lifu@cib.ac.cn.
authors.forename: Fu
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Chemistry, Women University of Azad Jammu and Kashmir, Bagh, 12500, Pakistan.
authors.forename: Ashfaq-Ahmad
authors.initials: AA
authors.lastname: Khan
authors.affiliation: Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing, 100026, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610013, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yu
authors.affiliation: School of Pharmacy and Bioengineering, Chengdu University, Chengdu, 610106, China; Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China. Electronic address: wangmk@cib.ac.cn.
authors.forename: Ming-Kui
authors.initials: MK
authors.lastname: Wang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002776:Cholestanes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D015394:Molecular Structure; D031441:Ophiopogon / Q000737:chemistry*; D035281:Plant Tubers / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013237:Stereoisomerism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002776:Cholestanes; D004365:Drugs, Chinese Herbal; D012503:Saponins
keywords: C(27)-steroidal saponins; C(29)-steroidal saponins; Cytotoxic activity; Liliaceae; Ophiopogon japonicus