title: Case report: when two and ½ men go camping….
abstract: In patients, rapid identification of bacterial species may help to guide treatment at early stages. New protocols for the identification directly from positive blood culture flasks significantly helped in the presented case report.
  
  
  
  Two patients (a father and son) presented with diarrhea, malaise, and fever of 3 to 4 days duration. Blood cultures from both patients cultured short Gram-positive rods. MALDI-TOF based rapid identification protocol direct from positive blood culture identified Listeria monocytogenes as the cause of sepsis and could be confirmed with conventional methods. Listeria monocytogenes was identified 24 h later by conventional biochemical identification methods (VITEK 2). Antibiotic treatment was adjusted early in response to the MALDI-TOF based identification of bacteremia. Pulsed field gel electrophoresis confirmed the suspected relatedness of the father's and son's isolates.
  
  
  
  MALDI-TOF based may provide a rapid identification of bacterial species directly from positive blood culture.
authors.affiliation: Division of Infectious Diseases and Hospital Epidemiology, University Hospital Basel, Basel, Switzerland.|Department of Internal Medicine, University Hospital Basel, Basel, Switzerland.
authors.forename: Matthias
authors.initials: M
authors.lastname: von Rotz
authors.affiliation: Division of Pediatric Infectious Diseases, Children University Hospital Basel, Basel, Switzerland.
authors.forename: Alexa
authors.initials: A
authors.lastname: Dierig
authors.affiliation: Division of Pediatric Infectious Diseases, Children University Hospital Basel, Basel, Switzerland.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Heininger
authors.affiliation: Department of Internal Medicine, University Hospital Basel, Basel, Switzerland.
authors.forename: Carl
authors.initials: C
authors.lastname: Chrobak
authors.affiliation: Division of Infectious Diseases and Hospital Epidemiology, University Hospital Basel, Basel, Switzerland.
authors.forename: Veronika
authors.initials: V
authors.lastname: Baettig
authors.affiliation: Applied Microbiology Research, Department of Biomedicine, University of Basel, Basel, Switzerland. adrian.egli@usb.ch.|Clinical Microbiology, University Hospital Basel, Basel, Switzerland. adrian.egli@usb.ch.
authors.forename: Adrian
authors.initials: A
authors.lastname: Egli
mesh_terms: D000293:Adolescent; D016470:Bacteremia / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D000071997:Blood Culture; D002165:Camping; D046508:Culture Techniques; D042241:Early Diagnosis; D061345:Early Medical Intervention; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D008089:Listeria monocytogenes / Q000302:isolation & purification*; D008088:Listeriosis / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D018805:Sepsis / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
keywords: Listeria monocytogenes; MALDI-TOF; Pulsed field gel electrophoresis; Rapid identification; Sepsis; Typing