title: Reversible manipulation of the G-quadruplex structures and enzymatic reactions through supramolecular host-guest interactions.
abstract: Supramolecular chemistry addresses intermolecular forces and consequently promises great flexibility and precision. Biological systems are often the inspirations for supramolecular research. The G-quadruplex (G4) belongs to one of the most important secondary structures in nucleic acids. Until recently, the supramolecular manipulation of the G4 has not been reported. The present study is the first to disclose a supramolecular switch for the reversible control of human telomere G4s. Moreover, this supramolecular switch has been successfully used to manipulate an enzymatic reaction. Using various methods, we show that cucurbit[7]uril preferably locks and encapsulates the positively charged piperidines of Razo through supramolecular interactions. They can switch the conformations of the DNA inhibitor between a flexible state and the rigid G4 and are therefore responsible for the reversible control of the thrombin activity. Thus, our findings open a promising route and exhibit potential applications in future studies of chemical biology.
authors.affiliation: College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan 430072, Hubei Province, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Tian
authors.affiliation: College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan 430072, Hubei Province, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Song
authors.affiliation: College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan 430072, Hubei Province, China.
authors.forename: Lai
authors.initials: L
authors.lastname: Wei
authors.affiliation: College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan 430072, Hubei Province, China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan 430072, Hubei Province, China.
authors.forename: Boshi
authors.initials: B
authors.lastname: Fu
authors.affiliation: College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan 430072, Hubei Province, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: He
authors.affiliation: College of Chemical Engineering and Technology, Wuhan University of Science and Technology, Wuhan 430081, Hubei Province, China.
authors.forename: Xi-Ran
authors.initials: XR
authors.lastname: Yang
authors.affiliation: College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan 430072, Hubei Province, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Wu
authors.affiliation: Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, Hubei Province, China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Xu
authors.affiliation: College of Chemical Engineering and Technology, Wuhan University of Science and Technology, Wuhan 430081, Hubei Province, China.
authors.forename: Si-Min
authors.initials: SM
authors.lastname: Liu
authors.affiliation: Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, Hubei Province, China.
authors.forename: Conggang
authors.initials: C
authors.lastname: Li
authors.affiliation: College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan 430072, Hubei Province, China.
authors.forename: Shaoru
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan 430072, Hubei Province, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
mesh_terms: D001391:Azo Compounds / Q000737:chemistry; D001483:Base Sequence; D001952:Bridged-Ring Compounds / Q000737:chemistry / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry*; D054856:G-Quadruplexes / Q000187:drug effects*; D006801:Humans; D007093:Imidazoles / Q000737:chemistry / Q000494:pharmacology*; D062105:Molecular Docking Simulation; D010880:Piperidines / Q000737:chemistry; D016615:Telomere / Q000737:chemistry / Q000187:drug effects; D013917:Thrombin / Q000737:chemistry* / Q000378:metabolism; D047028:Macrocyclic Compounds; D000099246:Cucurbit(n)urils
chemical_list: D001391:Azo Compounds; D001952:Bridged-Ring Compounds; D004247:DNA; D007093:Imidazoles; D010880:Piperidines; D013917:Thrombin; C456276:cucurbit(7)uril; D047028:Macrocyclic Compounds; D000099246:Cucurbit(n)urils; C009850:azobenzene