title: 2003-2013, a valuable study: Autologous tumor lysate-pulsed dendritic cell immunotherapy with cytokine-induced killer cells improves survival in stage IV breast cancer.
abstract: Dendritic cells (DCs) and cytokine-induced killer (CIK) cells have both shown activity as immunotherapy in some malignancies. Our aim was to prospective assess the effect of this immunotherapy in patients with stage IV breast cancer. Between Aug 2003 and Dec 2013, we collected 368 patients who met inclusion criteria and divided into immunotherapy group (treatment group: 188 patients) and chemotherapy group (control group: 180 patients). DCs were prepared from the mononuclear cells isolated from patients in the treatment group using IL-2/GM-CSF and were loaded with tumour antigens; CIK cells were prepared by incubating peripheral blood lymphocytes with IL-2, IFN-γ, and CD3 antibodies. After the patients had received low-dose chemotherapy, those in the treatment group also received the DC-CIK therapy, which was repeated four times in a fortnight to form one cycle. At least three cycles of DC-CIK therapy were given. Immune function was measured in treatment group patients' sera. Disease-free survival (DFS) and Overall survival (OS) after the diagnosis of stage IV breast cancer was assessed after a 10-year follow-up. The result demonstrated that immune function is obviously enhanced after DC-CIK therapy. By Cox regression analysis, DC-CIK therapy reduced the risk of disease progression (p<0.01) with an increased OS (p<0.01). After low-dose chemotherapy, active immunization with DC-CIK immunotherapy is a potentially effective approach for the control of tumour growth in stage IV breast cancer patients.
authors.affiliation: Department of Biotherapy Center, Fuda Cancer Hospital, Jinan University School of Medicine, NO. 2 TangDe Xi Rd., Tianhe District, Guangzhou, China; Department of Fuda Cancer Institute, Guangzhou, NO. 2 TangDe Xi Rd., Tianhe District, Guangzhou, China.
authors.forename: Mao
authors.initials: M
authors.lastname: Lin
authors.affiliation: Department of Biotherapy Center, Fuda Cancer Hospital, Jinan University School of Medicine, NO. 2 TangDe Xi Rd., Tianhe District, Guangzhou, China; Department of Fuda Cancer Institute, Guangzhou, NO. 2 TangDe Xi Rd., Tianhe District, Guangzhou, China.
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Liang
authors.affiliation: Department of Fuda Cancer Institute, Guangzhou, NO. 2 TangDe Xi Rd., Tianhe District, Guangzhou, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Jiang
authors.affiliation: Department of Biotherapy Center, Fuda Cancer Hospital, Jinan University School of Medicine, NO. 2 TangDe Xi Rd., Tianhe District, Guangzhou, China.
authors.forename: Jiongyuan
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Biotherapy Center, Fuda Cancer Hospital, Jinan University School of Medicine, NO. 2 TangDe Xi Rd., Tianhe District, Guangzhou, China.
authors.forename: Weibing
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Department of Biotherapy Center, Fuda Cancer Hospital, Jinan University School of Medicine, NO. 2 TangDe Xi Rd., Tianhe District, Guangzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qian
authors.affiliation: Department of Biotherapy Center, Fuda Cancer Hospital, Jinan University School of Medicine, NO. 2 TangDe Xi Rd., Tianhe District, Guangzhou, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Biotherapy Center, Fuda Cancer Hospital, Jinan University School of Medicine, NO. 2 TangDe Xi Rd., Tianhe District, Guangzhou, China.
authors.forename: Zhanchun
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Biotherapy Center, Fuda Cancer Hospital, Jinan University School of Medicine, NO. 2 TangDe Xi Rd., Tianhe District, Guangzhou, China; Department of Fuda Cancer Institute, Guangzhou, NO. 2 TangDe Xi Rd., Tianhe District, Guangzhou, China. Electronic address: linmaogz@163.com.
authors.forename: Jibing
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Fuda Cancer Institute, Guangzhou, NO. 2 TangDe Xi Rd., Tianhe District, Guangzhou, China. Electronic address: little.silly.ding@qq.com.
authors.forename: Lizhi
authors.initials: L
authors.lastname: Niu
authors.affiliation: Department of Fuda Cancer Institute, Guangzhou, NO. 2 TangDe Xi Rd., Tianhe District, Guangzhou, China.
authors.forename: Kecheng
authors.initials: K
authors.lastname: Xu
authors.affiliation: Peking University, Central Lab of Cancer Hospital, Beijing, China.
authors.forename: Youyong
authors.initials: Y
authors.lastname: Lv
mesh_terms: D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000276:immunology*; D001943:Breast Neoplasms / Q000175:diagnosis / Q000276:immunology* / Q000401:mortality / Q000628:therapy*; D019496:Cancer Vaccines / Q000276:immunology* / Q000627:therapeutic use; D003131:Combined Modality Therapy; D055612:Cytokine-Induced Killer Cells / Q000276:immunology* / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006801:Humans; D007167:Immunotherapy / Q000009:adverse effects / Q000379:methods; D053208:Kaplan-Meier Estimate; D018655:Lymphocyte Count; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D016016:Proportional Hazards Models; D012189:Retrospective Studies; D016896:Treatment Outcome
chemical_list: D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D016207:Cytokines
keywords: Cytokine-induced killer; DFS; Dendritic cell; Immunotherapy; OS; Stage IV breast cancer