title: Whole-genome resequencing of Bacillus cereus and expression of genes functioning in sodium chloride stress.
abstract: Bacillus cereus is one of the most common opportunistic pathogens responsible for various foodborn diseases. To investigate the regulatory mechanism of B. cereus under high osmotic pressure, two B. cereus strains B25 and B26 were isolated from the industrial soy sauce residue containing high-salt concentration. Resequencing was performed by Illumina/Solexa platform and 13,646 SNPs and 434 InDels were identified as common variants between B25 and B26 against reference genome, followed by COG, GO, and KEGG enrichment analysis. Furthermore, 49 key genes involving in Na+/H+,K+transporter, dipeptide or tripeptide transporter, stress response were selected and classified into 27 groups. Further validation was performed by qRT-PCR, and 4 candidate genes were found most associated with osmotic response. Gene expression of the 4 candidate genes was then analyzed accordingly, and down regulation was obtained for gene BC0669 and BC0754 associated with K+transport system. However, dramatic up regulation was detected for gene BC2114 involving in glutathione peroxidase, indicating the activation of antioxidant responses by osmotic stress via genetic regulation. As concluded, bioinformatic analysis and gene expression profile represented the basis of further investigation on the genetic and regulatory mechanism of bacterial salt tolerance.
authors.affiliation: School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Department of Microbial Pathogenesis, School of Dentistry, University of Maryland, Baltimore, MD, USA; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou, 510640, China. Electronic address: zhenbo.xu@hotmail.com.
authors.forename: Zhenbo
authors.initials: Z
authors.lastname: Xu
authors.affiliation: School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
authors.forename: Jinhong
authors.initials: J
authors.lastname: Xie
authors.affiliation: School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
authors.forename: Junyan
authors.initials: J
authors.lastname: Liu
authors.affiliation: School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Ji
authors.affiliation: School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
authors.forename: Thanapop
authors.initials: T
authors.lastname: Soteyome
authors.affiliation: Department of Clinical Pharmacy, College of Pharmacy, University of Tennessee Health Sciences Center, Memphis, TN 38163, USA.
authors.forename: Brian M
authors.initials: BM
authors.lastname: Peters
authors.affiliation: Department of Laboratory Medicine, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China.
authors.forename: Dingqiang
authors.initials: D
authors.lastname: Chen
authors.affiliation: School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou, 510640, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.affiliation: School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou, 510640, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Microbial Pathogenesis, School of Dentistry, University of Maryland, Baltimore, MD, USA.
authors.forename: Mark E
authors.initials: ME
authors.lastname: Shirtliff
mesh_terms: D001409:Bacillus cereus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology*; D005516:Food Microbiology; D020869:Gene Expression Profiling; D016680:Genome, Bacterial; D026901:Membrane Transport Proteins / Q000096:biosynthesis / Q000235:genetics; D009997:Osmotic Pressure; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000633:toxicity*; D013312:Stress, Physiological
chemical_list: D026901:Membrane Transport Proteins; D012965:Sodium Chloride
keywords: Bacillus cereus; Glutathione peroxidase; K(+) transport; Resequencing; Salt-tolerance