title: Hymenobacter daeguensis sp. nov. isolated from river water.
abstract: A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, aerobic bacterial strain, designated 16F3Y-2T, was isolated from the Han River, South Korea, and was characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain 16F3Y-2Tbelonged to the family Cytophagaceae in the phylum Bacteroidetes and was most closely related to 'Hymenobacter terrae' DG7A (98.01%), H. soli PB17T(97.26%), H. glaciei VUG-A130T(96.78%), H. antarcticus VUG-A42aaT(96.72%), H. ruber PB156T(96.61%), and H. saemangeumensis GSR0100T(95.77%). The G+C content of the genomic DNA of strain 16F3Y-2Twas 62.9 mol%. The isolate contained MK-7 as the predominant respiratory quinone, and summed feature 3 (C16:1ω7c/C16:1ω6c; 35.5%), C15:0iso (16.9%), C16:1ω5c (10.9%), and C15:0anteiso (9.9%) as major fatty acids. The major polar lipid was phosphatidylethanolamine. Phenotypic and chemotaxonomic data supported the affiliation of strain 16F3Y-2Twith the genus Hymenobacter. However, strain 16F3Y-2Texhibited relatively low levels of DNA-DNA relatedness with 'H. terrae' KCTC 32554 (44.1%) and H. soli KCTC 12607T(24.3%), clearly indicating that the isolate constitutes a new genospecies. Strain 16F3Y-2Tcould be differentiated from its phylogenetic neighbors on the basis of several phenotypic, genotypic, and chemotaxonomic features. Therefore, strain 16F3Y-2Trepresents a novel species in the genus Hymenobacter, for which the name Hymenobacter daeguensis sp. nov. is proposed. The type strain is 16F3Y-2T(=KCTC 52537T=JCM 31654T).
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Leonid N
authors.initials: LN
authors.lastname: Ten
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Yeon-Hee
authors.initials: YH
authors.lastname: Lee
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Jae-Jin
authors.initials: JJ
authors.lastname: Lee
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Park
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Seung-Yeol
authors.initials: SY
authors.lastname: Lee
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Sangkyu
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Environmental Engineering, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Dae Sung
authors.initials: DS
authors.lastname: Lee
authors.affiliation: Department of Horticultural Science, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: In-Kyu
authors.initials: IK
authors.lastname: Kang
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea. heeyoung@knu.ac.kr.|Institute of Plant Medicine, Kyungpook National University, Daegu, 41566, Republic of Korea. heeyoung@knu.ac.kr.
authors.forename: Hee-Young
authors.initials: HY
authors.lastname: Jung
mesh_terms: D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D001482:Base Composition; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D045483:Rivers / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S
keywords: Bacteroidetes; Hymenobacter; polyphasic taxonomy