title: Accumulation ofα-Synuclein in Cerebellar Purkinje Cells of Diabetic Rats and Its Potential Relationship with Inflammation and Oxidative Stress Markers.
abstract: Objective.The present study was conducted to evaluate the relationship between plasma oxidative stress markers such as malondialdehyde (MDA) and glutathione (GSH), inflammatory marker pentraxin-3 (PTX3), and cerebellar accumulation ofα-synuclein in streptozotocin- (STZ-) induced diabetes model in rats.Methods.Twelve rats were included in the study. Diabetes (n= 6) was induced with a single intraperitoneal injection of streptozotocin (STZ, 60 mg/kg). Diabetes was verified after 48 h by measuring blood glucose levels. Six rats served as controls. Following 8 weeks, rats were sacrificed for biochemical and immunohistochemical evaluation.Results.Plasma MDA levels were significantly higher in diabetic rats when compared with the control rats (p< 0.01), while plasma GSH levels were lower in the diabetic group than in the control group (p< 0.01). Also, plasma pentraxin-3 levels were statistically higher in diabetic rats than in the control rats (p< 0.01). The analysis of cerebellarα-synuclein immunohistochemistry showed a significant increase inα-synuclein immunoexpression in the diabetic group compared to the control group (p< 0.01).Conclusion.Due to increased inflammation and oxidative stress in the chronic period of hyperglycemia linked to diabetes, there may beα-synuclein accumulation in the cerebellum and the plasma PTX3 levels may be assessed as an important biomarker of this situation.
authors.affiliation: Department of Neurology, Trakya University Faculty of Medicine, Edirne, Turkey.
authors.forename: Volkan
authors.initials: V
authors.lastname: Solmaz
authors.affiliation: Department of Neurology, Trakya University Faculty of Medicine, Edirne, Turkey.
authors.forename: Hatice
authors.identifier: 0000-0003-4705-8499
authors.initials: H
authors.lastname: Köse Özlece
authors.affiliation: Department of Physiology, Kafkas University Faculty of Medicine, Kars, Turkey.
authors.forename: Hüseyin Avni
authors.initials: HA
authors.lastname: Eroglu
authors.affiliation: Department of Histology and Embryology, Ege University Faculty of Medicine, Izmir, Turkey.
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Aktuğ
authors.affiliation: Department of Physiology, Bilim University Faculty of Medicine, Istanbul, Turkey.
authors.forename: Oytun
authors.initials: O
authors.lastname: Erbaş
authors.affiliation: Department of Physiology, Ege University Faculty of Medicine, Izmir, Turkey.
authors.forename: Dilek
authors.identifier: 0000-0002-4505-0939
authors.initials: D
authors.lastname: Taşkıran