title: Anti-inflammatory effects of Dioscorea alata L. anthocyanins in a TNBS-induced colitis model.
abstract: The purple yam, Dioscorea alata L., is an important source of starch, vitamins and polyphenols. Five different pigments from the purple tubers of this plant were separated by high-performance liquid chromatography-mass spectrometry, and the anthocyanin fraction (DACN) was collected. The anti-inflammatory effects of DACNs were investigated at different concentrations and compared with the standard colitis treatment, 5-aminosalicylic acid, in a trinitrobenzenesulfonic acid (TNBS)-induced colitis mouse model. Macro- and microscopic parameters including body weight change, disease activity index (DAI) and intestinal histology were used for the determination of the anti-inflammatory effects of DACNs. The gene expression levels of tight junction-related proteins in the intestine, myeloperoxidase activity, inducible nitric oxide synthase activity in colonic tissues and pro-inflammatory cytokine production in serum were also measured to elucidate the mechanism of DACN action. Eighty micrograms of DACNs per kilogram of body weight produced potent anti-inflammatory effects in the mouse model of inflammatory bowel disease (IBD), as shown by the DAI (2.78 ± 0.38 vs. 0.44 ± 0.51). Therefore, DACNs may be applied as a potential food supplement in IBD therapy.
authors.affiliation: Laboratory of Tropical Animal Breeding, Reproduction and Nutrition, Hainan University, Haikou, Hainan, China. 007zhanghaiwen@163.com xmwangsnow@sina.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: Laboratory of Tropical Animal Breeding, Reproduction and Nutrition, Hainan University, Haikou, Hainan, China. 007zhanghaiwen@163.com xmwangsnow@sina.com.
authors.forename: Shihui
authors.initials: S
authors.lastname: Hu
authors.affiliation: Laboratory of Tropical Animal Breeding, Reproduction and Nutrition, Hainan University, Haikou, Hainan, China. 007zhanghaiwen@163.com xmwangsnow@sina.com and Key Laboratory of Tropical Biological Resources of Ministry of Education, Haikou 570228, China.
authors.forename: Haiwen
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Laboratory of Tropical Animal Breeding, Reproduction and Nutrition, Hainan University, Haikou, Hainan, China. 007zhanghaiwen@163.com xmwangsnow@sina.com.
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Guan
authors.affiliation: Laboratory of Tropical Animal Breeding, Reproduction and Nutrition, Hainan University, Haikou, Hainan, China. 007zhanghaiwen@163.com xmwangsnow@sina.com.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Laboratory of Tropical Animal Breeding, Reproduction and Nutrition, Hainan University, Haikou, Hainan, China. 007zhanghaiwen@163.com xmwangsnow@sina.com and Key Laboratory of Tropical Biological Resources of Ministry of Education, Haikou 570228, China.
authors.forename: Xuemei
authors.identifier: http://orcid.org/0000-0001-8452-8846
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000008:administration & dosage*; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D027742:Dioscorea / Q000737:chemistry*; D004195:Disease Models, Animal; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010936:Plant Extracts / Q000008:administration & dosage*; D014302:Trinitrobenzenesulfonic Acid / Q000009:adverse effects
chemical_list: D000872:Anthocyanins; D000893:Anti-Inflammatory Agents; D016207:Cytokines; D010936:Plant Extracts; D014302:Trinitrobenzenesulfonic Acid