title: The effects of icariin on the expression of HIF-1α, HSP-60 and HSP-70 in PC12 cells suffered from oxygen-glucose deprivation-induced injury.
abstract: BACKGROUND
  The effects of icariin, a chief constituent of ﬂavonoids from Epimedium brevicornum Maxim (Berberidaceae), on the levels of HIF-1α, HSP-60 and HSP-70 remain unknown.
  
  
  OBJECTIVE
  To explore the effects of icariin on the levels of HSP-60, HIF-1α and HSP-70 neuron-specific enolase (NSE) and cell viability.
  
  
  METHODS
  PC12 cells were treated with icariin (10-7, 10-6or 10-5mol/L) for 3 h (1 h before oxygen-glucose deprivation (OGD) plus 2 h OGD). HSP-60, HIF-1α, HSP-70 and NSE were measured using enzyme-linked immunosorbent assay (ELISA). Cell viability was determined by metabolic 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
  
  
  RESULTS
  After 2 h OGD, levels of HIF-1α, HSP-60, HSP-70 and NSE were increased significantly (HIF-1α: 33.3 ± 1.9 ng/L, HSP-60: 199 ± 16 ng/L, HSP-70: 195 ± 17 ng/L, NSE: 1487 ± 125 ng/L), and cell viability was significantly decreased (0.26 ± 0.03), while icariin (10-7, 10-6, or 10-5mol/L) significantly reduced the contents of HIF-1α, HSP-60, HSP-70 and NSE (HIF-1α: 14.1 ± 1.4, 22.6 ± 1.8, 15.7 ± 2.1, HSP-60: 100 ± 12, 89 ± 6, 113 ± 11, HSP-70: 139 ± 9, 118 ± 7, 95 ± 9 and NSE: 1121 ± 80, 1019 ± 52, 731 ± 88), and improved cell viability (0.36 ± 0.03, 0.38 ± 0.04, 0.37 ± 0.03) in OGD-treated PC12 cells.
  
  
  CONCLUSIONS
  These results indicate that the protective mechanisms of icariin against OGD-induced injury may be related to down-regulating the expression of HIF-1α, HSP-60 and HSP-70.
authors.affiliation: a Department of Pharmacology of Zhuhai Campus , Zunyi Medical University , Zhuhai , Guangdong , China.
authors.forename: Zhen-Tao
authors.initials: ZT
authors.lastname: Mo
authors.affiliation: a Department of Pharmacology of Zhuhai Campus , Zunyi Medical University , Zhuhai , Guangdong , China.
authors.forename: Wen-Na
authors.initials: WN
authors.lastname: Li
authors.affiliation: a Department of Pharmacology of Zhuhai Campus , Zunyi Medical University , Zhuhai , Guangdong , China.
authors.forename: Yu-Rong
authors.initials: YR
authors.lastname: Zhai
authors.affiliation: a Department of Pharmacology of Zhuhai Campus , Zunyi Medical University , Zhuhai , Guangdong , China.
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Gao
mesh_terms: D000818:Animals; D015687:Cell Hypoxia; D002470:Cell Survival / Q000187:drug effects; D018834:Chaperonin 60 / Q000032:analysis*; D015536:Down-Regulation; D004797:Enzyme-Linked Immunosorbent Assay; D005419:Flavonoids / Q000494:pharmacology*; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000032:analysis*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016716:PC12 Cells; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D051381:Rats
chemical_list: D018834:Chaperonin 60; D005419:Flavonoids; D018840:HSP70 Heat-Shock Proteins; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D018696:Neuroprotective Agents; D010751:Phosphopyruvate Hydratase; C056599:icariin
keywords: Heat shock protein; cell viability; neuron-specific enolase