title: Validation of a monoclonal antibody-based immunofluorescent assay to detect Burkholderia pseudomallei in blood cultures.
abstract: BACKGROUND
  Identification of Burkholderia pseudomallei, the cause of melioidosis, using routine methods takes several days. Use of a monoclonal antibody-based immunofluorescent assay (IFA) on positive blood cultures may speed diagnosis.
  
  
  METHODS
  We tested the diagnostic accuracy of the IFA on 545 blood cultures positive for Gram-negative organisms at Udon Thani Hospital, Thailand, between June 2015 and August 2016.
  
  
  RESULTS
  Sensitivity of the IFA was 100% and specificity was 99.6%. The median decrease in time to pathogen identification between the IFA result and routine methods was 28 h (IQR 25-51), p<0.0001.
  
  
  CONCLUSIONS
  The IFA accurately expedites the diagnosis of melioidosis.
authors.affiliation: Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.
authors.forename: Adul
authors.initials: A
authors.lastname: Dulsuk
authors.affiliation: Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.
authors.forename: Suporn
authors.initials: S
authors.lastname: Paksanont
authors.affiliation: Department of Medical Technology, Udon Thani Regional Hospital, Udon Thani, 41000, Thailand.
authors.forename: Adisak
authors.initials: A
authors.lastname: Sangchankoom
authors.affiliation: Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.
authors.forename: Peeraya
authors.initials: P
authors.lastname: Ekchariyawat
authors.affiliation: Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.
authors.forename: Rungnapa
authors.initials: R
authors.lastname: Phunpang
authors.affiliation: Department of Medical Technology, Udon Thani Regional Hospital, Udon Thani, 41000, Thailand.
authors.forename: Yaowaruk
authors.initials: Y
authors.lastname: Jutrakul
authors.affiliation: Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.|Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.
authors.forename: Narisara
authors.initials: N
authors.lastname: Chantratita
authors.affiliation: Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.|Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Washington, Seattle, WA, 98104-2499, USA.|International Respiratory and Severe Illness Center, University of Washington, Seattle, WA, 98104, USA.
authors.forename: T Eoin
authors.initials: TE
authors.lastname: West
mesh_terms: D000071997:Blood Culture; D016957:Burkholderia pseudomallei / Q000302:isolation & purification; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D008554:Melioidosis / Q000175:diagnosis
keywords: 
            Burkholderia pseudomallei
          ; Bacteremia; Diagnosis; Immunofluorescent assay; Melioidosis; Thailand