title: KIAA1377is associated with lymph node metastasis in esophageal squamous cell carcinoma.
abstract: KIAA1377, of which there are few studies regarding cell biology and neurological diseases, has been found to be significantly amplified in esophageal squamous cell carcinoma (ESCC) with lymph node metastasis compared with ESCC without lymph node metastasis. This suggests thatKIAA1377may play a role in the lymph node metastasis of ESCC. There has, to the best of our knowledge, been no study performed to investigate the role ofKIAA1377in ESCC. In the present study, the expression ofKIAA1377was detected by immunohistochemistry, and its expression was statistically analyzed with clinicopathological parameters, using commercially obtained tissue arrays consisting of 86 cases of ESCC and 79 paired controls.KIAA1377was knocked downex vivousing transient transfection with specific small hairpin RNA (shRNA) vectors into ESCC TE-1 and EC9706 cell lines whose endogenousKIAA1377level was highest. The variation of proliferation, migration and invasion were evaluated using methyl thiazolyl tetrazolium, wound healing and Transwell assay, respectively. It was foundin vivothatKIAA1377expression was significantly associated with lymph node metastasis and differentiation, andex vivothat knockdown ofKIAA1377cannot significantly affect proliferation and mobility in the ESCC cell line TE-1. Overall, this is the first study suggesting thatKIAA1377may play a role in the lymph node micrometastasis of ESCC.
authors.affiliation: Clinical Medical Research Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China; State Key Lab Incubation Base of Xinjiang Major Diseases Research, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
authors.forename: Shu-Tao
authors.initials: ST
authors.lastname: Zheng
authors.affiliation: Clinical Medical Research Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China; State Key Lab Incubation Base of Xinjiang Major Diseases Research, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
authors.forename: Chen-Chen
authors.initials: CC
authors.lastname: Yang
authors.affiliation: Clinical Medical Research Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China; State Key Lab Incubation Base of Xinjiang Major Diseases Research, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Clinical Medical Research Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China; State Key Lab Incubation Base of Xinjiang Major Diseases Research, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.affiliation: Clinical Medical Research Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China; State Key Lab Incubation Base of Xinjiang Major Diseases Research, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
authors.forename: Mang
authors.initials: M
authors.lastname: Lu
authors.affiliation: Clinical Medical Research Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China; State Key Lab Incubation Base of Xinjiang Major Diseases Research, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Dai
authors.affiliation: Clinical Medical Research Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China; State Key Lab Incubation Base of Xinjiang Major Diseases Research, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
authors.forename: Xiang-Peng
authors.initials: XP
authors.lastname: Gao
authors.affiliation: State Key Lab Incubation Base of Xinjiang Major Diseases Research, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
authors.forename: Ilyar
authors.initials: I
authors.lastname: Sheyhidin
authors.affiliation: Clinical Medical Research Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China; State Key Lab Incubation Base of Xinjiang Major Diseases Research, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Lu
keywords: ESCC; KIAA1377; lymph node metastasis