title: Flavonoids with hepatoprotective activity from the leaves of Cleome viscosa L.
abstract: One new flavonol glycoside named visconoside C (1), together with seven known flavonol glycosides, quercetin 3-O-β-d-glucopyranoside 7-O-α-l-rhamnopyranoside (2), quercetin 7-O-α-l-rhamnopyranoside (3), astragalin (4), kaempferol 3-O-(4-O-acetyl)-α-l-rhamnopyranoside (5), kaempferol 7-O-α-l-rhamnopyranoside (6), kaempferitrin (7) and kaempferol 3-O-β-d-glucopyranoside 7-O-α-l-rhamnopyranoside (8) were isolated by various chromatography methods from the leaves of Cleome viscosa L. Their structures were elucidated by IR, UV, HR-ESI-MS and NMR (1D & 2D) experiments. The cytotoxicity and hepatoprotective activities using HepG2 human hepatoma cell line of 1 were measured by MTT assay. At the concentration of 25 μM and 50 μM, 1 showed cytotoxic activity against HepG2 cells (cell viability was decreased to 22.2 and 23.0%, respectively, compared with doxorubicin control), while at the concentration of 100 μM, 1 showed hepatoprotective activity against CCl4-induced hepatotoxicity on HepG2 cells (34.3%, compared with quercetin control).
authors.affiliation: a Institute of Chemical Technology , Vietnam Academy of Science and Technology , Ho Chi Minh city , Viet Nam.
authors.forename: Tan Phat
authors.initials: TP
authors.lastname: Nguyen
authors.affiliation: b Tay Do University , Can Tho city , Viet Nam.
authors.forename: Cong Luan
authors.initials: CL
authors.lastname: Tran
authors.affiliation: c Dalat Research Center for Cultivation and Processing of Medicinal Plants, Ministry of Health , Viet Nam.
authors.forename: Chi Hung
authors.initials: CH
authors.lastname: Vuong
authors.affiliation: d Faculty of Pharmacy, Department of Pharmacology , University of Medicine and Pharmacy , Ho Chi Minh City , Viet Nam.
authors.forename: Thi Hong Tuoi
authors.initials: THT
authors.lastname: Do
authors.affiliation: e Institute of Applied Materials Science , Vietnam Academy of Science and Technology , Ho Chi Minh city , Viet Nam.
authors.forename: Tien Dung
authors.initials: TD
authors.lastname: Le
authors.affiliation: a Institute of Chemical Technology , Vietnam Academy of Science and Technology , Ho Chi Minh city , Viet Nam.
authors.forename: Dinh Tri
authors.initials: DT
authors.lastname: Mai
authors.affiliation: a Institute of Chemical Technology , Vietnam Academy of Science and Technology , Ho Chi Minh city , Viet Nam.
authors.forename: Nhat Minh
authors.initials: NM
authors.lastname: Phan
mesh_terms: D031485:Cleome / Q000737:chemistry*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D006027:Glycosides / Q000737:chemistry / Q000494:pharmacology; D056945:Hep G2 Cells; D006801:Humans; D044949:Kaempferols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008099:Liver / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry; D020011:Protective Agents / Q000737:chemistry* / Q000494:pharmacology*; D021241:Spectrometry, Mass, Electrospray Ionization; D014744:Vietnam
chemical_list: D005419:Flavonoids; D006027:Glycosides; D044949:Kaempferols; D020011:Protective Agents; C516876:kaempferol-7-O-alpha-L-rhamnopyranoside; C042728:lespenefril
keywords: Cleome viscosa; capparaceae; cytotoxicity activity; hepatoprotective activity; visconoside C