title: Transcriptome Sequencing of Gracilariopsis lemaneiformis to Analyze the Genes Related to Optically Active Phycoerythrin Synthesis.
abstract: Gracilariopsis lemaneiformis (aka Gracilaria lemaneiformis) is a red macroalga rich in phycoerythrin, which can capture light efficiently and transfer it to photosystemⅡ. However, little is known about the synthesis of optically active phycoerythrinin in G. lemaneiformis at the molecular level. With the advent of high-throughput sequencing technology, analysis of genetic information for G. lemaneiformis by transcriptome sequencing is an effective means to get a deeper insight into the molecular mechanism of phycoerythrin synthesis. Illumina technology was employed to sequence the transcriptome of two strains of G. lemaneiformis- the wild type and a green-pigmented mutant. We obtained a total of 86915 assembled unigenes as a reference gene set, and 42884 unigenes were annotated in at least one public database. Taking the above transcriptome sequencing as a reference gene set, 4041 differentially expressed genes were screened to analyze and compare the gene expression profiles of the wild type and green mutant. By GO and KEGG pathway analysis, we concluded that three factors, including a reduction in the expression level of apo-phycoerythrin, an increase of chlorophyll light-harvesting complex synthesis, and reduction of phycoerythrobilin by competitive inhibition, caused the reduction of optically active phycoerythrin in the green-pigmented mutant.
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, Qingdao, Shandong, China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Huang
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, Qingdao, Shandong, China.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Zang
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, Qingdao, Shandong, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wu
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, Qingdao, Shandong, China.
authors.forename: Yuming
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, Qingdao, Shandong, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, Qingdao, Shandong, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, Qingdao, Shandong, China.
authors.forename: Yating
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, Qingdao, Shandong, China.
authors.forename: Bangxiang
authors.initials: B
authors.lastname: He
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, Qingdao, Shandong, China.
authors.forename: Dongfang
authors.initials: D
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, Qingdao, Shandong, China.
authors.forename: Xinwei
authors.initials: X
authors.lastname: Song
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, Qingdao, Shandong, China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Liu
mesh_terms: D002734:Chlorophyll / Q000378:metabolism; D030562:Databases, Protein; D020869:Gene Expression Profiling; D063990:Gene Ontology; D056726:Genetic Association Studies; D008027:Light; D045342:Light-Harvesting Protein Complexes / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics; D058977:Molecular Sequence Annotation; D009154:Mutation / Q000235:genetics; D055620:Optical Phenomena; D010085:Oxidative Phosphorylation; D010788:Photosynthesis / Q000235:genetics; D010799:Phycoerythrin / Q000096:biosynthesis*; D060888:Real-Time Polymerase Chain Reaction; D000461:Rhodophyta / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods*; D013050:Spectrometry, Fluorescence; D059467:Transcriptome / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D045342:Light-Harvesting Protein Complexes; D010799:Phycoerythrin; D002734:Chlorophyll