title: Two types of albino mutants in desert and migratory locusts are caused by gene defects in the same signaling pathway.
abstract: Albinism is caused by mutations in the genes involved in melanin production. Albino nymphs of Locusta migratoria and Schistocerca gregaria reared under crowded conditions are uniformly creamy-white in color. However, nothing is known about the molecular mechanisms underlying this phenomenon in locusts. The albino strain of L. migratoria is known to lack the dark-color-inducing neuropeptide corazonin (Crz). In this study, we report that this albino strain has a 10-base-pair deletion in the gene LmCRZ, which encodes Crz. This mutation was found to cause a frame-shift, resulting in a null mutation in Crz. On the other hand, the albino strain of S. gregaria is known to have an intact Crz. This strain was found to possess a single-nucleotide substitution in the middle of the Crz receptor-encoding gene, SgCRZR, which caused a nonsense mutation, resulting in a truncated receptor. Silencing of SgCRZR in wild-type S. gregaria nymphs greatly reduced the area and intensity of their black patterning, suggesting that the functional defect of SgCRZR likely causes the albinism. The expression level of SgCRZR in the albino S. gregaria was comparable to that in the wild type. Unlike the wild type, the albino strain of this locust did not show a phase-dependent shift in a morphometric trait controlled by Crz. From these results, we conclude that the mutations in LmCRZ and SgCRZR are responsible for the albinism in L. migratoria and S. gregaria, respectively, indicating that the two types of albinism are caused by different genetic defects in the same Crz signaling pathway.
authors.affiliation: National Agriculture and Food Research Organization, Institute of Agrobiological Sciences, 1-2 Ohwashi, Tsukuba, Ibaraki 305-8634, Japan. Electronic address: rsugahara@affrc.go.jp.
authors.forename: Ryohei
authors.initials: R
authors.lastname: Sugahara
authors.affiliation: National Agriculture and Food Research Organization, Institute of Agrobiological Sciences, 1-2 Ohwashi, Tsukuba, Ibaraki 305-8634, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Tanaka
authors.affiliation: National Agriculture and Food Research Organization, Institute of Agrobiological Sciences, 1-2 Ohwashi, Tsukuba, Ibaraki 305-8634, Japan.
authors.forename: Akiya
authors.initials: A
authors.lastname: Jouraku
authors.affiliation: National Agriculture and Food Research Organization, Institute of Agrobiological Sciences, 1-2 Ohwashi, Tsukuba, Ibaraki 305-8634, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Shiotsuki
mesh_terms: D000417:Albinism / Q000235:genetics*; D025041:Animal Migration; D000818:Animals; D003889:Desert Climate; D006110:Grasshoppers / Q000033:anatomy & histology / Q000145:classification / Q000235:genetics*; D019476:Insect Proteins / Q000235:genetics; D048188:Locusta migratoria / Q000235:genetics*; D050505:Mutant Proteins / Q000235:genetics; D009154:Mutation; D009479:Neuropeptides / Q000235:genetics; D010858:Pigmentation / Q000235:genetics*; D018013:Receptors, Neuropeptide / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics
chemical_list: D019476:Insect Proteins; D050505:Mutant Proteins; D009479:Neuropeptides; D018013:Receptors, Neuropeptide; C468120:corazonin receptor; C060602:corazonin protein, insect
keywords: Albinism; Corazonin gene; Corazonin receptor gene; Density-dependent phase polyphenism; Desert locust; Migratory locust