title: Mitochondrial dysfunction, oxidative stress and apoptotic induction in microglial BV-2 cells treated with sodium arsenate.
abstract: The treatment of microglial BV-2 cells with sodium arsenate (As(V): 0.1-400μmol/L - 48hr) induces a dose-dependent response. The neurotoxic effects of high concentrations of As(V) (100, 200 and 400μmol/L) are characterized by increased levels of mitochondrial complexes I, II, and IV followed by increased superoxide anion generation. Moreover, As(V) triggers an apoptotic mode of cell death, demonstrated by an apoptotic SubG1 peak, associated with an alteration of plasma membrane integrity. There is also a decrease in transmembrane mitochondrial potential and mitochondrial adenosine triphosphate ATP. It is therefore tempting to speculate that As(V) triggers mitochondrial dysfunction, which may lead to defective oxidative phosphorylation subsequently causing mitochondrial oxidative damage, which in turn induces an apoptotic mode of cell death.
authors.affiliation: Univ. Bourgogne Franche-Comté Laboratory Bio-PeroxIL, Biochemistry of the Peroxisome, Inflammation and Lipid Metabolism EA7270/INSERM, Faculty of Sciences Gabriel, Dijon 21000, France; Laboratory of Nutrition-Functional Foods and Vascular Diseases, Faculty of Medicine, University of Monastir, Monastir 5019, Tunisia. Electronic address: wafa.kharroubi@hotmail.com.
authors.forename: Wafa
authors.initials: W
authors.lastname: Kharroubi
authors.affiliation: Univ. Bourgogne Franche-Comté Laboratory Bio-PeroxIL, Biochemistry of the Peroxisome, Inflammation and Lipid Metabolism EA7270/INSERM, Faculty of Sciences Gabriel, Dijon 21000, France; Laboratory of Nutrition-Functional Foods and Vascular Diseases, Faculty of Medicine, University of Monastir, Monastir 5019, Tunisia.
authors.forename: Samia
authors.initials: S
authors.lastname: Haj Ahmed
authors.affiliation: Univ. Bourgogne Franche-Comté Laboratory Bio-PeroxIL, Biochemistry of the Peroxisome, Inflammation and Lipid Metabolism EA7270/INSERM, Faculty of Sciences Gabriel, Dijon 21000, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Nury
authors.affiliation: Univ. Bourgogne Franche-Comté Laboratory Bio-PeroxIL, Biochemistry of the Peroxisome, Inflammation and Lipid Metabolism EA7270/INSERM, Faculty of Sciences Gabriel, Dijon 21000, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Andreoletti
authors.affiliation: Laboratory of Nutrition-Functional Foods and Vascular Diseases, Faculty of Medicine, University of Monastir, Monastir 5019, Tunisia.
authors.forename: Rachid
authors.initials: R
authors.lastname: Sakly
authors.affiliation: Laboratory of Nutrition-Functional Foods and Vascular Diseases, Faculty of Medicine, University of Monastir, Monastir 5019, Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Hammami
authors.affiliation: Univ. Bourgogne Franche-Comté Laboratory Bio-PeroxIL, Biochemistry of the Peroxisome, Inflammation and Lipid Metabolism EA7270/INSERM, Faculty of Sciences Gabriel, Dijon 21000, France.
authors.forename: Gérard
authors.initials: G
authors.lastname: Lizard
mesh_terms: D000255:Adenosine Triphosphate; D000818:Animals; D017209:Apoptosis; D001149:Arsenates / Q000633:toxicity*; D002460:Cell Line; D015386:Hazardous Substances / Q000633:toxicity*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D008928:Mitochondria / Q000187:drug effects*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects
chemical_list: D001149:Arsenates; D015386:Hazardous Substances; C009277:sodium arsenate; D000255:Adenosine Triphosphate
keywords: Apoptosis; Microglial BV-2 cells; Mitochondrial dysfunction; Oxidative phosphorylation complexes; Sodium arsenate; Superoxide anions