title: The change in serum Thiol/Disulphide homeostasis after transrectal ultrasound guided prostate biopsy.
abstract: OBJECTIVE
  The aim of this prospective clinical study was to investigate variations in a novel oxidative stress marker (thiol/disulphide homeostasis) in men who underwent transrectal ultrasound guided prostate biopsy (TRUSB).
  
  
  METHODS
  A total of 22 men undergoing TRUSB of the prostate were enrolled in the study. Patients with abnormal digital rectal examination and/or total prostate specific antigen (PSA) over 4ng/mL underwent TRUSB with 12 cores. Serum samples were obtained before and just after the procedure to evaluate the possible changes in thiol/disulphide homeostasis. Mean age, total PSA and free PSA, prostate volume and histopathological data were also recorded.
  
  
  RESULTS
  Mean age of the study population was 65.05±8.89 years. Significant decreases in native and total thiol levels were documented after the biopsy procedure. However, serum disulphide levels and disulphide/native thiol, disulphide/total thiol and native / total thiol ratios did not significantly change after TRUSB. No correlation was observed between oxidative parameters and total PSA and free PSA levels, prostate volume and histopathology of the prostate. However, mean patient age was significantly correlated with mean native and total thiol levels.
  
  
  CONCLUSIONS
  Significant decreases in serum native and total thiol levels related to the prostate biopsy procedure suggest that TRUSB causes acute oxidative stress in the human body. Since our trial is the first in the current literature to investigate these oxidative stress markers in urology practice, additional studies are warranted.
authors.affiliation: Department of Urology, Antalya Training and Research Hospital, Antalya, Turkey.
authors.forename: Hüsnü
authors.initials: H
authors.lastname: Tokgöz
authors.affiliation: Department of Urology, Antalya Training and Research Hospital, Antalya, Turkey.
authors.forename: Selim
authors.initials: S
authors.lastname: Taş
authors.affiliation: Department of Biochemistry , Antalya Training and Research Hospital, Antalya, Turkey.
authors.forename: Özlem
authors.initials: Ö
authors.lastname: Giray
authors.affiliation: Department of Urology, Antalya Training and Research Hospital, Antalya, Turkey.
authors.forename: Soner
authors.initials: S
authors.lastname: Yalçınkaya
authors.affiliation: Department of Radiology, Antalya Training and Research Hospital, Antalya, Turkey.
authors.forename: Özlem
authors.initials: Ö
authors.lastname: Tokgöz
authors.affiliation: Department of Biochemistry, Yildirim Beyazit University, School of Medicine, Ankara, Turkey.
authors.forename: Cemile
authors.initials: C
authors.lastname: Koca
authors.affiliation: Department of Urology, Antalya Training and Research Hospital, Antalya, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Savaş
authors.affiliation: Department of Biochemistry, Yildirim Beyazit University, School of Medicine, Ankara, Turkey.
authors.forename: Özcan
authors.initials: Ö
authors.lastname: Erel
mesh_terms: D000368:Aged; D015415:Biomarkers; D001706:Biopsy; D051517:Digital Rectal Examination; D004220:Disulfides / Q000378:metabolism*; D061765:Endoscopic Ultrasound-Guided Fine Needle Aspiration; D006801:Humans; D061705:Image-Guided Biopsy; D008297:Male; D018384:Oxidative Stress; D011446:Prospective Studies; D011467:Prostate / Q000473:pathology; D017430:Prostate-Specific Antigen / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D014463:Ultrasonography
chemical_list: D015415:Biomarkers; D004220:Disulfides; D013438:Sulfhydryl Compounds; D017430:Prostate-Specific Antigen
keywords: Biopsy; Oxidative Stress; Prostate