title: Graphene oxide nanolayers as nanoparticle anchors on biomaterial surfaces with nanostructures and charge balance for bone regeneration.
abstract: Graphene oxide (GO) is a carbon-based nanomaterial with high surface area and abundant functional groups, providing various sites for binding and immobilization of growth factor vehicles. This study used GO nanolayer as an anchor for the immobilization of bone morphogenetic protein-2 (BMP-2)-encapsulated bovine serum albumin nanoparticles (NPs) on the hydroxyapatite (HA) and tricalcium phosphate (TCP) scaffolds by electrostatic interaction between the positive charges of the NPs and negative charges of GO. GO nanolayers prevented the rapid degradation of TCP scaffolds. Moreover, GO nanolayers promoted NP adsorption on these scaffolds, and realized BMP-2 sustained release. NPs endowed the scaffold surfaces with a nanostructure similar to that of the extracellular matrix (ECM), improving bone marrow stromal cell (BMSC) attachment. Furthermore, the positive charged NPs and negative charged GO nanolayers constructed a charge-balanced surface on the scaffolds, enhancing BMSC proliferation. The nanostructure, charge balance and BMP-2 sustained release capability synergistically improved BMSC differentiation and bone regeneration. In summary, GO is a potential candidate to modify biomaterial surfaces as an anchor for efficient immobilization of growth factor vehicles. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1311-1323, 2017.
authors.affiliation: Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan, 610031, China.
authors.forename: Chaoming
authors.initials: C
authors.lastname: Xie
authors.affiliation: Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan, 610031, China.
authors.forename: Honglong
authors.initials: H
authors.lastname: Sun
authors.affiliation: National Engineering Research Center for Biomaterials, Genome Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan, 610064, China.
authors.forename: Kefeng
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Chengdu Military General Hospital, Chengdu, Sichuan, 610083, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan, 610031, China.|National Engineering Research Center for Biomaterials, Genome Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan, 610064, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Materials Science and Engineering, South University of Science and Technology, Shenzhen, Guangdong, 518055, China.
authors.forename: Fuzeng
authors.initials: F
authors.lastname: Ren
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry / Q000494:pharmacology; D001861:Bone Regeneration / Q000187:drug effects*; D002417:Cattle; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D006108:Graphite / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry / Q000627:therapeutic use; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000494:pharmacology
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D020099:Coated Materials, Biocompatible; D012710:Serum Albumin, Bovine; D006108:Graphite
keywords: BSA nanoparticle; drug delivery; graphene oxide; growth factors; tissue engineering scaffold