title: Honeybee locomotion is impaired by Am-CaV3 low voltage-activated Ca2+channel antagonist.
abstract: Voltage-gated Ca2+channels are key transducers of cellular excitability and participate in several crucial physiological responses. In vertebrates, 10 Ca2+channel genes, grouped in 3 families (CaV1, CaV2 and CaV3), have been described and characterized. Insects possess only one member of each family. These genes have been isolated in a limited number of species and very few have been characterized although, in addition to their crucial role, they may represent a collateral target for neurotoxic insecticides. We have isolated the 3 genes coding for the 3 Ca2+channels expressed in Apis mellifera. This work provides the first detailed characterization of the honeybee T-type CaV3 Ca2+channel and demonstrates the low toxicity of inhibiting this channel. Comparing Ca2+currents recorded in bee neurons and myocytes with Ca2+currents recorded in Xenopus oocytes expressing the honeybee CaV3 gene suggests native expression in bee muscle cells only. High-voltage activated Ca2+channels could be recorded in the somata of different cultured bee neurons. These functional data were confirmed by in situ hybridization, immunolocalization and in vivo analysis of the effects of a CaV3 inhibitor. The biophysical and pharmacological characterization and the tissue distribution of CaV3 suggest a role in honeybee muscle function.
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247, CNRS ENSCM, Université de Montpellier, bât. CRBM, 1919 Route de Mende, 34293 Montpellier cedex 5, France.
authors.forename: M
authors.initials: M
authors.lastname: Rousset
authors.affiliation: INRA, UR 406 Abeilles et Environnement, 228 Route de l'aérodrome, Domaine Saint Paul, Site Agroparc, CS40509, 84914 Avignon cedex 9, France.
authors.forename: C
authors.initials: C
authors.lastname: Collet
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247, CNRS ENSCM, Université de Montpellier, bât. CRBM, 1919 Route de Mende, 34293 Montpellier cedex 5, France.
authors.forename: T
authors.initials: T
authors.lastname: Cens
authors.affiliation: Evolution Génomes Comportement et Ecologie, CNRS, Univ. Paris-Sud, IRD, Université Paris Saclay, 1 avenue de la Terrasse, F-91198 Gif-sur-Yvette, France.
authors.forename: F
authors.initials: F
authors.lastname: Bastin
authors.affiliation: RCIM, UPRES-EA2647 USC INRA 1330, SFR QUASAV 4207, Université d'Angers, 2 Bld Lavoisier, 49045 ANGERS Cedex 01, France.
authors.forename: V
authors.initials: V
authors.lastname: Raymond
authors.affiliation: CRCA, UMR 5169, CNRS, Université Paul Sabatier, Bâtiment IVR3, 118 route de Narbonne, 31062 Toulouse, France.
authors.forename: I
authors.initials: I
authors.lastname: Massou
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247, CNRS ENSCM, Université de Montpellier, bât. CRBM, 1919 Route de Mende, 34293 Montpellier cedex 5, France.
authors.forename: C
authors.initials: C
authors.lastname: Menard
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247, CNRS ENSCM, Université de Montpellier, bât. CRBM, 1919 Route de Mende, 34293 Montpellier cedex 5, France.
authors.forename: J-B
authors.initials: JB
authors.lastname: Thibaud
authors.affiliation: INRA, UR 406 Abeilles et Environnement, 228 Route de l'aérodrome, Domaine Saint Paul, Site Agroparc, CS40509, 84914 Avignon cedex 9, France.
authors.forename: M
authors.initials: M
authors.lastname: Charreton
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247, CNRS ENSCM, Université de Montpellier, bât. CRBM, 1919 Route de Mende, 34293 Montpellier cedex 5, France.
authors.forename: M
authors.initials: M
authors.lastname: Vignes
authors.affiliation: Centre de recherche, Institut universitaire en santé mentale de Québec, 2601 Chemin de la Canardière, Québec (Québec), G1J 2G3, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Chahine
authors.affiliation: Evolution Génomes Comportement et Ecologie, CNRS, Univ. Paris-Sud, IRD, Université Paris Saclay, 1 avenue de la Terrasse, F-91198 Gif-sur-Yvette, France.
authors.forename: J C
authors.initials: JC
authors.lastname: Sandoz
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247, CNRS ENSCM, Université de Montpellier, bât. CRBM, 1919 Route de Mende, 34293 Montpellier cedex 5, France.
authors.forename: P
authors.initials: P
authors.lastname: Charnet
mesh_terms: D000818:Animals; D001516:Bees / Q000187:drug effects* / Q000502:physiology*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D020747:Calcium Channels, T-Type / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D008124:Locomotion / Q000187:drug effects*; D020748:Mibefradil / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D009830:Olfactory Bulb / Q000187:drug effects / Q000502:physiology; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism; D014981:Xenopus
chemical_list: D002121:Calcium Channel Blockers; D020747:Calcium Channels, T-Type; D020748:Mibefradil