title: The PufX quinone channel enables the light-harvesting 1 antenna to bind more carotenoids for light collection and photoprotection.
abstract: Photosynthesis in some phototrophic bacteria requires the PufX component of the reaction centre-light-harvesting 1-PufX (RC-LH1-PufX) complex, which creates a pore for quinone/quinol (Q/QH2) exchange across the LH1 barrier surrounding the RC. However, photosynthetic bacteria such as Thermochromatium (T.) tepidum do not require PufX because there are fewer carotenoid binding sites, which creates multiple pores in the LH1 ring for Q/QH2exchange. We show that an αTrp-24→Phe alteration of the Rhodobacter (Rba.) sphaeroides LH1 antenna impairs carotenoid binding and allows photosynthetic growth in the absence of PufX. We propose that acquisition of PufX and confining Q/QH2traffic to a pore adjacent to the RC QBsite is an evolutionary upgrade that allows increased LH1 carotenoid content for enhanced light absorption and photoprotection.
authors.affiliation: Department of Molecular Biology and Biotechnology, University of Sheffield, UK.
authors.forename: John D
authors.initials: JD
authors.lastname: Olsen
authors.affiliation: Department of Molecular Biology and Biotechnology, University of Sheffield, UK.
authors.forename: Elizabeth C
authors.initials: EC
authors.lastname: Martin
authors.affiliation: Department of Molecular Biology and Biotechnology, University of Sheffield, UK.
authors.forename: C Neil
authors.initials: CN
authors.lastname: Hunter
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001429:Bacteriochlorophylls / Q000378:metabolism; D016227:Benzoquinones / Q000378:metabolism*; D002338:Carotenoids / Q000378:metabolism*; D008027:Light; D045342:Light-Harvesting Protein Complexes / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010788:Photosynthesis / Q000235:genetics / Q000528:radiation effects; D011485:Protein Binding; D012242:Rhodobacter sphaeroides / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D013053:Spectrophotometry
chemical_list: D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; D016227:Benzoquinones; D045342:Light-Harvesting Protein Complexes; C088749:PufX protein, Rhodobacter; D002338:Carotenoids; C004532:quinone
keywords: bacterial photosynthesis; carotenoid; light-harvesting; membrane protein; quinones, reaction centre