title: Effects of rosa mosqueta oil supplementation in lipogenic markers associated with prevention of liver steatosis.
abstract: Rosa mosqueta (RM) oil is rich in α-linolenic acid (ALA) - a precursor of eicosapentaenoic (EPA) and docosahexaenoic acid (DHA), and it has a high antioxidant activity due to its abundant content of tocopherols. Additionally, it has been observed that RM oil administration prevents hepatic steatosis. Thus, the aim of this study was to demonstrate the antilipogenic mechanism related to RM oil administration in a high-fat diet (HFD) fed mice model by evaluating markers associated with the regulation of lipid droplet metabolism (PLIN2, PLIN5 and PPAR-γ), and proteins associated with lipogenesis (FAS and SREBP-1c). C57BL/6J mice were fed either a control diet or a HFD, with and without RM oil supplementation for 12 weeks. The results showed that RM oil supplementation decreases hepatic PLIN2 and PPAR-γ mRNA expression and SREBP-1c, FAS and PLIN2 protein levels, whereas we did not find changes in the level of PLIN5 among the groups. These results suggest that modulation of lipogenic markers could be one of the mechanisms, through which RM oil supplementation prevents the hepatic steatosis induced by HFD consumption in a mice model.
authors.affiliation: Molecular and Clinical Pharmacology Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile. gtapia@med.uchile.cl.
authors.forename: Camila G
authors.initials: CG
authors.lastname: Dossi
authors.affiliation: Molecular and Clinical Pharmacology Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile. gtapia@med.uchile.cl.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Cadagan
authors.affiliation: Molecular and Clinical Pharmacology Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile. gtapia@med.uchile.cl.
authors.forename: Marcela
authors.initials: M
authors.lastname: San Martín
authors.affiliation: Department of Medical Technology, Faculty of Medicine, University of Chile, Santiago, Chile.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Espinosa
authors.affiliation: Molecular and Clinical Pharmacology Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile. gtapia@med.uchile.cl.
authors.forename: Daniel
authors.initials: D
authors.lastname: González-Mañán
authors.affiliation: Molecular and Clinical Pharmacology Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile. gtapia@med.uchile.cl.
authors.forename: David
authors.initials: D
authors.lastname: Silva
authors.affiliation: School of Biochemical Engineering, Faculty of Engineering, Pontifical Catholic University of Valparaiso, Valparaiso, Chile.
authors.forename: Rodrigo A
authors.initials: RA
authors.lastname: Mancilla
authors.affiliation: Molecular and Clinical Pharmacology Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile. gtapia@med.uchile.cl.
authors.forename: Gladys S
authors.identifier: http://orcid.org/0000-0002-5229-2696
authors.initials: GS
authors.lastname: Tapia
mesh_terms: D000818:Animals; D059305:Diet, High-Fat / Q000009:adverse effects; D019587:Dietary Supplements / Q000032:analysis; D005234:Fatty Liver / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control*; D006801:Humans; D050155:Lipogenesis; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D000070780:Perilipin-2 / Q000235:genetics / Q000378:metabolism; D000070797:Perilipin-5 / Q000235:genetics / Q000378:metabolism; D010938:Plant Oils / Q000008:administration & dosage*; D027826:Rosa / Q000737:chemistry*; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics / Q000378:metabolism
chemical_list: D047495:PPAR gamma; D000070780:Perilipin-2; D000070797:Perilipin-5; D010938:Plant Oils; C000602983:Plin2 protein, mouse; C511805:Plin5 protein, mouse; D051780:Sterol Regulatory Element Binding Protein 1