title: Curcumin inhibits the survival and metastasis of prostate cancer cells via the Notch-1 signaling pathway.
abstract: Prostate cancer is one of the most common malignancies in men, and it urgently demands precise interventions that target the signaling pathways implicated in its initiation, progression, and metastasis. The Notch-1 signaling pathway is closely associated with the pathophysiology of prostate cancer. This study investigated the antitumor effects and mechanisms of curcumin, which is a well-known natural compound from curcuminoids, in prostate cancer cells. Viability, proliferation, and migration were analyzed in two prostate cancer cell lines, DU145 and PC3, after curcumin treatment. Whether the Notch-1 signaling pathway is involved in the antitumor effects of curcumin was examined. Curcumin inhibited the survival and proliferation of PC3 and DU145 cells in a dose- and time-dependent manner and inhibited DU145 migration. Curcumin did not affect the expression of Notch-1 or its active product NICD, but it did inhibit the expression of MT1-MMP and MMP2 proteins in DU145 cells. We found that curcumin inhibited the DNA-binding ability of NICD in DU145 cells. In conclusion, curcumin inhibited the survival and metastasis of prostate cancer cells via the Notch-1 signaling pathway.
authors.affiliation: Department of Urological Surgery, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Jingzhe
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Urological Surgery, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Chengli
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Urological Surgery, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Urological Surgery, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Urological Surgery, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Yusen
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Institute of Basic Research in Clinical Evaluation, China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Urological Surgery, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Kong
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D003474:Curcumin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D053511:Matrix Metalloproteinase 14 / Q000032:analysis; D020778:Matrix Metalloproteinase 2 / Q000032:analysis; D009362:Neoplasm Metastasis / Q000503:physiopathology; D011471:Prostatic Neoplasms / Q000503:physiopathology; D051881:Receptor, Notch1 / Q000032:analysis*; D015398:Signal Transduction; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents; C497761:NOTCH1 protein, human; D051881:Receptor, Notch1; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C507086:MMP14 protein, human; D053511:Matrix Metalloproteinase 14; D003474:Curcumin
keywords: Curcumin; Notch signaling pathway; prostate cancer