title: Optimized conditions for preserving stability and integrity of porcine circovirus type2 virus-like particles during long-term storage.
abstract: Although porcine circovirus type 2 (PCV2) virus-like particles (VLPs) have been successfully harvested from various protein expression systems, conditions to promote their stability and integrity during long-term storage have not been well defined since only the intact VLPs, instead of the monomeric capsid protein (Cap), can induce neutralizing antibodies in pigs in previous studies. In this study, freshly prepared PCV2 VLPs were stored in several media (various concentrations of NaCl, sorbitol, sucrose and trehalose) at three temperatures (4°C, -20°C and -80°C) and their stability and integration were evaluated after 7 month. Addition of 15% trehalose in storage buffer promoted long-term preservation of PCV2 VLPs. In contrast, storage buffer with 5% osmolytes (sucrose, trehalose and sorbitol) did not confer stabilization for long-term storage. These refined storage conditions for stabilization of PCV2 VLPs should enhance their use in vaccines.
authors.affiliation: Laboratory of Functional Proteomics (LFP) and Research Center of Reverse Vaccinology (RCRV), College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China.
authors.forename: Naidong
authors.initials: N
authors.lastname: Wang
authors.affiliation: Laboratory of Functional Proteomics (LFP) and Research Center of Reverse Vaccinology (RCRV), College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Laboratory of Functional Proteomics (LFP) and Research Center of Reverse Vaccinology (RCRV), College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China.
authors.forename: Xinnuo
authors.initials: X
authors.lastname: Lei
authors.affiliation: Laboratory of Functional Proteomics (LFP) and Research Center of Reverse Vaccinology (RCRV), College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China.
authors.forename: Wanting
authors.initials: W
authors.lastname: Yu
authors.affiliation: Laboratory of Functional Proteomics (LFP) and Research Center of Reverse Vaccinology (RCRV), College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhan
authors.affiliation: Laboratory of Functional Proteomics (LFP) and Research Center of Reverse Vaccinology (RCRV), College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China.
authors.forename: Dongliang
authors.initials: D
authors.lastname: Wang
authors.affiliation: Laboratory of Functional Proteomics (LFP) and Research Center of Reverse Vaccinology (RCRV), College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China.
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Laboratory of Functional Proteomics (LFP) and Research Center of Reverse Vaccinology (RCRV), College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China.
authors.forename: Aibing
authors.initials: A
authors.lastname: Wang
authors.affiliation: College of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Lehui
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: jiangp@njau.edu.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Jiang
authors.affiliation: Laboratory of Functional Proteomics (LFP) and Research Center of Reverse Vaccinology (RCRV), College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China. Electronic address: yiyang@hunau.edu.cn.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000818:Animals; D017925:Circovirus / Q000276:immunology / Q000648:ultrastructure*; D003451:Cryoprotective Agents; D004356:Drug Storage / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D012965:Sodium Chloride / Q000378:metabolism; D013552:Swine; D013696:Temperature; D014765:Viral Vaccines / Q000276:immunology; D022701:Virosomes / Q000276:immunology / Q000648:ultrastructure*
chemical_list: D003451:Cryoprotective Agents; D014765:Viral Vaccines; D022701:Virosomes; D012965:Sodium Chloride
keywords: Osmolytes; Porcine circovirus type 2 (PCV2); Stability; Virus-like particles (VLPs)