title: Dynamic Optical Contrast Imaging.
abstract: The variable location and indistinct features of parathyroid glands can make their intraoperative identification challenging. Currently, there exists no routine use of localization methods during surgery. Dynamic optical contrast imaging (DOCI) leverages a novel realization of temporally dependent measurements of tissue autofluorescence that allows the acquisition of specific tissue properties. A prospective series of patients with primary hyperparathyroidism was examined. Parathyroid lesions and surrounding tissues were collected; fluorescence decay images were acquired via DOCI. Ex vivo samples (81 patients) were processed for histologic assessment. DOCI extracts relative fluorescence decay information in a surgically relevant field of view with a clinically accessible acquisition time <2 minutes. Analysis of DOCI revealed microscopic characterization sufficient for tissue type identification consistent with histology ( P < .05). DOCI is capable of efficiently distinguishing parathyroid tissue from adjacent tissues. Such an intraoperative tool would be transformative, helping surgeons to identify lesions, preserve healthy tissue, and improve patient outcomes.
authors.affiliation: 1 Department of Head and Neck Surgery, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, California, USA.|2 Head and Neck Cancer Program, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, California, USA.
authors.forename: Irene A
authors.initials: IA
authors.lastname: Kim
authors.affiliation: 2 Head and Neck Cancer Program, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, California, USA.|3 Department of Bioengineering, Henry Samueli School of Engineering and Applied Sciences, University of California-Los Angeles, Los Angeles, California, USA.|4 Department of Surgery, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, California, USA.
authors.forename: Zachary D
authors.initials: ZD
authors.lastname: Taylor
authors.affiliation: 3 Department of Bioengineering, Henry Samueli School of Engineering and Applied Sciences, University of California-Los Angeles, Los Angeles, California, USA.
authors.forename: Harrison
authors.initials: H
authors.lastname: Cheng
authors.affiliation: 1 Department of Head and Neck Surgery, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, California, USA.|2 Head and Neck Cancer Program, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, California, USA.
authors.forename: Christine
authors.initials: C
authors.lastname: Sebastian
authors.affiliation: 3 Department of Bioengineering, Henry Samueli School of Engineering and Applied Sciences, University of California-Los Angeles, Los Angeles, California, USA.
authors.forename: Ashkan
authors.initials: A
authors.lastname: Maccabi
authors.affiliation: 3 Department of Bioengineering, Henry Samueli School of Engineering and Applied Sciences, University of California-Los Angeles, Los Angeles, California, USA.
authors.forename: James
authors.initials: J
authors.lastname: Garritano
authors.affiliation: 1 Department of Head and Neck Surgery, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, California, USA.|2 Head and Neck Cancer Program, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, California, USA.
authors.forename: Bobby
authors.initials: B
authors.lastname: Tajudeen
authors.affiliation: 1 Department of Head and Neck Surgery, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, California, USA.|2 Head and Neck Cancer Program, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, California, USA.
authors.forename: Ali
authors.initials: A
authors.lastname: Razfar
authors.affiliation: 5 Department of Pathology, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, California, USA.
authors.forename: Fernando
authors.initials: F
authors.lastname: Palma Diaz
authors.affiliation: 4 Department of Surgery, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, California, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Yeh
authors.affiliation: 6 Department of Electrical Engineering, Henry Samueli School of Engineering and Applied Sciences, University of California-Los Angeles, Los Angeles, California, USA.
authors.forename: Oscar
authors.initials: O
authors.lastname: Stafsudd
authors.affiliation: 3 Department of Bioengineering, Henry Samueli School of Engineering and Applied Sciences, University of California-Los Angeles, Los Angeles, California, USA.|4 Department of Surgery, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, California, USA.|6 Department of Electrical Engineering, Henry Samueli School of Engineering and Applied Sciences, University of California-Los Angeles, Los Angeles, California, USA.
authors.forename: Warren
authors.initials: W
authors.lastname: Grundfest
authors.affiliation: 1 Department of Head and Neck Surgery, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, California, USA.|2 Head and Neck Cancer Program, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, California, USA.|7 Jonsson Comprehensive Cancer Center, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, California, USA.
authors.forename: Maie
authors.initials: M
authors.lastname: St John
mesh_terms: D006801:Humans; D049950:Hyperparathyroidism, Primary / Q000000981:diagnostic imaging / Q000601:surgery; D061848:Optical Imaging; D010280:Parathyroid Glands / Q000033:anatomy & histology* / Q000000981:diagnostic imaging*
keywords: adenoma; hyperplasia; imaging; localization; parathyroid surgery