title: Functional characterization of a gibberellin receptor and its application in alfalfa biomass improvement.
abstract: Bioactive gibberellins (GAs) are essential phytohormones involved in the regulation of many aspects of plant development. GA receptors are crucial in GA signal transduction in plants. The GA receptor GoGID1 promotes plant elongation and improves biomass production when ectopically expressed in tobacco. Here, we discovered that GoGID1 can interact with the DELLA proteins of Arabidopsis in the presence of gibberellic acid. GoGID1 partially or completely functionally rescued the phenotypes of the Arabidopsis double-mutants atgid1a/atgid1c and atgid1a/atgid1b. The overexpression of GoGID1 led to increases in plant height and biomass production in transgenic Arabidopsis plants. The GoGID1 gene enhanced GA sensitivity of the transgenic plants. More importantly, transgenic alfalfa plants overexpressing GoGID1 exhibited increased growth rates, heights and biomass and produced larger leaves when compared with the control plants. Thus, GoGID1 functions as a GA receptor, playing multiple roles in plant growth and development. The GoGID1 gene has the potential to be used in the genetic engineering of forage crops for biomass improvement.
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Xuemin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.|Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot 010010, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Ban
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Yudi
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Animal Husbandry and Veterinary institute, Shanxi Academy of Agricultural Sciences, Taiyuan 030032, China.
authors.forename: Xinming
authors.initials: X
authors.lastname: Wu
authors.affiliation: Animal Husbandry and Veterinary institute, Shanxi Academy of Agricultural Sciences, Taiyuan 030032, China.
authors.forename: Yunqi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Wen
authors.affiliation: N.I.Vavilov All-Russian Research Institute of Plant Industry, St. Petersburg 190000, Russia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Chapurin
authors.affiliation: N.I.Vavilov All-Russian Research Institute of Plant Industry, St. Petersburg 190000, Russia.
authors.forename: Nikolay
authors.initials: N
authors.lastname: Dzyubenko
authors.affiliation: Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot 010010, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Li
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Zan
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Hongwen
authors.initials: H
authors.lastname: Gao
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics*; D018533:Biomass; D018506:Gene Expression Regulation, Plant; D005875:Gibberellins / Q000235:genetics* / Q000378:metabolism; D000455:Medicago sativa / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D063245:Plant Development / Q000235:genetics; D010937:Plant Growth Regulators / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development; D011956:Receptors, Cell Surface / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; C109555:GAI protein, Arabidopsis; C512225:GID1a protein, Arabidopsis; D005875:Gibberellins; D010937:Plant Growth Regulators; D011956:Receptors, Cell Surface