title: Integrins are required for tissue organization and restriction of neurogenesis in regenerating planarians.
abstract: Tissue regeneration depends on proliferative cells and on cues that regulate cell division, differentiation, patterning and the restriction of these processes once regeneration is complete. In planarians, flatworms with high regenerative potential, muscle cells express some of these instructive cues. Here, we show that members of the integrin family of adhesion molecules are required for the integrity of regenerating tissues, including the musculature. Remarkably, in regeneratingβ1-integrinRNAi planarians, we detected increased numbers of mitotic cells and progenitor cell types, as well as a reduced ability of stem cells and lineage-restricted progenitor cells to accumulate at wound sites. These animals also formed ectopic spheroid structures of neural identity in regenerating heads. Interestingly, those polarized assemblies comprised a variety of neural cells and underwent continuous growth. Our study indicates that integrin-mediated cell adhesion is required for the regenerative formation of organized tissues and for restricting neurogenesis during planarian regeneration.
authors.affiliation: Max Planck Research Group Stem Cells & Regeneration, Max Planck Institute for Molecular Biomedicine, Von-Esmarch-Str. 54, Münster 48149, Germany.|Medical Faculty, University of Münster, Albert-Schweitzer-Campus 1, 48149 Münster, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Seebeck
authors.affiliation: Max Planck Research Group Stem Cells & Regeneration, Max Planck Institute for Molecular Biomedicine, Von-Esmarch-Str. 54, Münster 48149, Germany.|Medical Faculty, University of Münster, Albert-Schweitzer-Campus 1, 48149 Münster, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: März
authors.affiliation: Max Planck Research Group Stem Cells & Regeneration, Max Planck Institute for Molecular Biomedicine, Von-Esmarch-Str. 54, Münster 48149, Germany.|Medical Faculty, University of Münster, Albert-Schweitzer-Campus 1, 48149 Münster, Germany.
authors.forename: Anna-Wiebke
authors.initials: AW
authors.lastname: Meyer
authors.affiliation: Max Planck Research Group Stem Cells & Regeneration, Max Planck Institute for Molecular Biomedicine, Von-Esmarch-Str. 54, Münster 48149, Germany.|Medical Faculty, University of Münster, Albert-Schweitzer-Campus 1, 48149 Münster, Germany.
authors.forename: Hanna
authors.initials: H
authors.lastname: Reuter
authors.affiliation: Max Planck Research Group Stem Cells & Regeneration, Max Planck Institute for Molecular Biomedicine, Von-Esmarch-Str. 54, Münster 48149, Germany.|Medical Faculty, University of Münster, Albert-Schweitzer-Campus 1, 48149 Münster, Germany.
authors.forename: Matthias C
authors.initials: MC
authors.lastname: Vogg
authors.affiliation: Flow Cytometry Unit, Max Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, Münster 48149, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Stehling
authors.affiliation: Electron Microscopy Unit, Max Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, Münster 48149, Germany.
authors.forename: Karina
authors.initials: K
authors.lastname: Mildner
authors.affiliation: Electron Microscopy Unit, Max Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, Münster 48149, Germany.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Zeuschner
authors.affiliation: Max Planck Research Group Stem Cells & Regeneration, Max Planck Institute for Molecular Biomedicine, Von-Esmarch-Str. 54, Münster 48149, Germany.|Medical Faculty, University of Münster, Albert-Schweitzer-Campus 1, 48149 Münster, Germany.
authors.forename: Franziska
authors.initials: F
authors.lastname: Rabert
authors.affiliation: Max Planck Research Group Stem Cells & Regeneration, Max Planck Institute for Molecular Biomedicine, Von-Esmarch-Str. 54, Münster 48149, Germany kerstin.bartscherer@mpi-muenster.mpg.de.|Medical Faculty, University of Münster, Albert-Schweitzer-Campus 1, 48149 Münster, Germany.
authors.forename: Kerstin
authors.identifier: 0000-0002-3070-4389
authors.initials: K
authors.lastname: Bartscherer
mesh_terms: D000818:Animals; D019521:Body Patterning; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D019012:Integrin beta1 / Q000502:physiology*; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000166:cytology; D010802:Phylogeny; D010932:Planarians / Q000502:physiology*; D034622:RNA Interference; D012038:Regeneration / Q000502:physiology*; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology
chemical_list: D019012:Integrin beta1
keywords: Ectospheres; Integrins; Patterning; Planaria; Regeneration; Stem cells