title: Designing a synchrotron micro-focusing beamline for macromolecular crystallography.
abstract: After a successful 10 years of operation, the Canadian Macromolecular Crystallography Facility 08ID-1 beamline will undergo an upgrade to establish micro-beam capability. This paper is mostly focussed on optics and computer simulations for ray tracing of the beamline. After completion, the focussed beam at the sample will have a much smaller size of 50 × 5 µm2(H x V), allowing measurement of X-ray diffraction patterns from much smaller crystals than possible presently. The beamline will be equipped with a fast sample changer and an ultra-low noise photon counting detector, allowing shutter-less operation of the beamline. Additionally, it will be possible to perform in-situ room-temperature experiments.
authors.affiliation: Canadian Light Source Inc., 44 Innovation Boulevard, Saskatoon, SK S7N 2V3, Canada.|College of Pharmacy and Nutrition, University of Saskatchewan, SK S7N 5C9, Canada.
authors.forename: Paweł
authors.initials: P
authors.lastname: Grochulski
authors.affiliation: Department of Biochemistry, University of Saskatchewan, Saskatoon, SK S7N 5E5, Canada.
authors.forename: Mirosław
authors.initials: M
authors.lastname: Cygler
authors.affiliation: Canadian Light Source Inc., 44 Innovation Boulevard, Saskatoon, SK S7N 2V3, Canada.
authors.forename: Brian
authors.initials: B
authors.lastname: Yates
mesh_terms: D018360:Crystallography, X-Ray / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D017356:Synchrotrons
keywords: X-ray optics; macromolecular crystallography beamline; structural biology; synchrotron instrumentation; synchrotron radiation