title: The Long Non-Coding RNA RHPN1-AS1 Promotes Uveal Melanoma Progression.
abstract: Increasing evidence suggests that aberrant long non-coding RNAs (lncRNAs) are significantly correlated with the pathogenesis, development and metastasis of cancers.RHPN1 antisense RNA 1(RHPN1-AS1) is a 2030-bp transcript originating from human chromosome 8q24. However, the role ofRHPN1-AS1in uveal melanoma (UM) remains to be clarified. In this study, we aimed to elucidate the molecular function ofRHPN1-AS1in UM. The RNA levels ofRHPN1-AS1in UM cell lines were examined using the quantitative real-time polymerase chain reaction (qRT-PCR). Short interfering RNAs (siRNAs) were designed to quenchRHPN1-AS1expression, and UM cells stably expressing short hairpin (sh)RHPN1-AS1were established. Next, the cell proliferation and migration abilities were determined using a colony formation assay and a transwell migration/invasion assay. A tumor xenograft model in nude mice was established to confirm the function ofRHPN1-AS1in vivo.RHPN1-AS1was significantly upregulated in a number of UM cell lines compared with the normal human retinal pigment epithelium (RPE) cell line.RHPN1-AS1knockdown significantly inhibited UM cell proliferation and migration in vitro and in vivo. Our data suggest thatRHPN1-AS1could be an oncoRNA in UM, which may serve as a candidate prognostic biomarker and target for new therapies in malignant UM.
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. drlulinna@126.com.
authors.forename: Linna
authors.initials: L
authors.lastname: Lu
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. yuxiaoyunzb@163.com.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. a789leilei@126.com.
authors.forename: Leilei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. abcdingxia@126.com.
authors.forename: Xia
authors.initials: X
authors.lastname: Ding
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. xypanhui@163.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Pan
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. winston-a@hotmail.com.
authors.forename: Xuyang
authors.initials: X
authors.lastname: Wen
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. 15721201988@163.com.
authors.forename: Shiqiong
authors.initials: S
authors.lastname: Xu
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. fishashorejessica@163.com.
authors.forename: Yue
authors.initials: Y
authors.lastname: Xing
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. fanjiayan1118@126.com.
authors.forename: Jiayan
authors.initials: J
authors.lastname: Fan
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. geshengfang@sjtu.edu.cn.
authors.forename: Shengfang
authors.initials: S
authors.lastname: Ge
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. zhanghe@sjtu.edu.cn.
authors.forename: He
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. jrb19760517@hotmail.com.
authors.forename: Renbing
authors.initials: R
authors.lastname: Jia
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. fanxq@sh163.net.
authors.forename: Xianqun
authors.initials: X
authors.lastname: Fan
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D003593:Cytoplasm / Q000235:genetics; D018450:Disease Progression; D015536:Down-Regulation / Q000235:genetics; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D063990:Gene Ontology; D015894:Genome, Human; D006801:Humans; D008545:Melanoma / Q000098:blood supply / Q000235:genetics* / Q000473:pathology*; D009361:Neoplasm Invasiveness; D009389:Neovascularization, Pathologic / Q000235:genetics; D062085:RNA, Long Noncoding / Q000235:genetics*; D034741:RNA, Small Interfering / Q000378:metabolism; D015854:Up-Regulation / Q000235:genetics; D014604:Uveal Neoplasms / Q000098:blood supply / Q000235:genetics* / Q000473:pathology*; D023041:Xenograft Model Antitumor Assays; D000098943:Uveal Melanoma
chemical_list: D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering; C000617856:long noncoding RNA RHPN1-AS1, human
keywords: RHPN1-AS1; lncRNA; migration; uveal melanoma