title: Analysis of glycero-lysophospholipids in gastric cancerous ascites.
abstract: Lysophosphatidic acid (LysoPA) has been proposed to be involved in the pathogenesis of various cancers. Moreover, glycero-lysophospholipids (glycero-LysoPLs) other than LysoPA are now emerging as novel lipid mediators. Therefore, we aimed to elucidate the possible involvement of glycero-LysoPLs in the pathogenesis of gastric cancer by measuring glycero-LysoPLs, autotaxin (ATX), and phosphatidylserine-specific phospholipase A1 (PS-PLA1) in ascites obtained from patients with gastric cancer and those with cirrhosis (as a control). We observed that after adjustments according to the albumin levels, the lysophosphatidylserine (LysoPS) and lysophosphatidylglycerol (LysoPG) levels were significantly higher, while the LysoPA and ATX levels were lower, in the ascites from patients with gastric cancer. We also found that multiple regression analyses revealed that ATX was selected as a significant explanatory factor for all the detectable LysoPA species only in the cirrhosis group and that a significant positive correlation was observed between LysoPS and PS-PLA1only in the gastric cancer group. In conclusion, the LysoPA levels might be determined largely by LysoPC and LysoPI (possible precursors) and the PS-PLA1-mediated pathway might be involved in the production of LysoPS in gastric cancer. Glycero-LysoPLs other than LysoPA might also be involved in the pathogenesis of cancer directly or through being converted into LysoPA.
authors.affiliation: Department of Surgical Oncology, University of Tokyo, Tokyo, Japan.
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Emoto
authors.affiliation: Departments of Clinical Laboratory Medicine and Gastrointestinal Surgery, University of Tokyo, Tokyo, Japan; CREST, Japan Science and Technology Corporation (JST).
authors.forename: Makoto
authors.initials: M
authors.lastname: Kurano
authors.affiliation: CREST, Japan Science and Technology Corporation (JST); Laboratory of Molecular and Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Tohoku University, Miyagi, Japan.
authors.forename: Kuniyuki
authors.initials: K
authors.lastname: Kano
authors.affiliation: Kanamecho Hospital, Tokyo, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Matsusaki
authors.affiliation: Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
authors.forename: Hiroharu
authors.initials: H
authors.lastname: Yamashita
authors.affiliation: Departments of Clinical Laboratory Medicine and Gastrointestinal Surgery, University of Tokyo, Tokyo, Japan; CREST, Japan Science and Technology Corporation (JST).
authors.forename: Masako
authors.initials: M
authors.lastname: Nishikawa
authors.affiliation: Bioscience Division, TOSOH Corporation, Kanagawa, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Igarashi
authors.affiliation: Departments of Clinical Laboratory Medicine and Gastrointestinal Surgery, University of Tokyo, Tokyo, Japan; CREST, Japan Science and Technology Corporation (JST).
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ikeda
authors.affiliation: CREST, Japan Science and Technology Corporation (JST); Laboratory of Molecular and Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Tohoku University, Miyagi, Japan.
authors.forename: Junken
authors.initials: J
authors.lastname: Aoki
authors.affiliation: Department of Surgical Oncology, University of Tokyo, Tokyo, Japan; Department of Gastrointestinal Surgery, Jichi Medical University, Tochigi, Japan.
authors.forename: Joji
authors.initials: J
authors.lastname: Kitayama
authors.affiliation: Departments of Clinical Laboratory Medicine and Gastrointestinal Surgery, University of Tokyo, Tokyo, Japan; CREST, Japan Science and Technology Corporation (JST). Electronic address: yatoyuta-tky@umin.ac.jp.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yatomi
mesh_terms: D000818:Animals; D001201:Ascites / Q000378:metabolism / Q000473:pathology; D005260:Female; D005355:Fibrosis / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008246:Lysophospholipids / Q000302:isolation & purification / Q000378:metabolism*; D008297:Male; D051379:Mice; D054466:Phospholipases A1 / Q000235:genetics / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D000099189:Lysophospholipase D
chemical_list: D008246:Lysophospholipids; D054466:Phospholipases A1; D010727:Phosphoric Diester Hydrolases; D000099189:Lysophospholipase D; C026223:lysophosphatidylglycerol; C025059:lysophosphatidylserine; C512665:PLA1A protein, human; C032881:lysophosphatidic acid
keywords: ascites; autotaxin; cirrhosis; gastric cancer