title: Complete genome sequence of a novel mitovirus from the phytopathogenic fungus Rhizoctonia oryzae-sativae.
abstract: A double-stranded RNA (dsRNA) segment was isolated from the filamentous phytopathogenic fungus Rhizoctonia oryzae-sativae and its full-length cDNA sequence (3038 nucleotides) was determined. Sequence analysis revealed that a large open reading frame (ORF) is present on the positive strand of this dsRNA segment when the mitochondrial genetic code was applied. The ORF encodes a putative RNA-dependent RNA polymerase, which shares the closest similarity with Rhizoctonia mitovirus 1 and Rhizophagus sp. RF1 mitovirus, with 43% and 29% identity, respectively. This dsRNA segment represents the replication form of a novel mitovirus that was temporarily designated Rhizoctonia oryzae-sativae mitovirus 1 (RoMV1). Phylogenetic analysis further suggested that RoMV1 belongs to the family Narnaviridae. This is the first study to report a mitovirus genome sequence in the phytopathogenic fungus R. oryzae-sativae.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.|Provincial Key Laboratory of Plant Pathology of Hubei Province, Plant Pathology Department, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.
authors.forename: Assane Hamidou
authors.initials: AH
authors.lastname: Abdoulaye
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.|Provincial Key Laboratory of Plant Pathology of Hubei Province, Plant Pathology Department, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.
authors.forename: Jiasen
authors.initials: J
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Fu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.|Provincial Key Laboratory of Plant Pathology of Hubei Province, Plant Pathology Department, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.
authors.forename: Daohong
authors.initials: D
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China. jiataoxie@mail.hzau.edu.cn.|Provincial Key Laboratory of Plant Pathology of Hubei Province, Plant Pathology Department, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China. jiataoxie@mail.hzau.edu.cn.
authors.forename: Jiatao
authors.initials: J
authors.lastname: Xie
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D016679:Genome, Viral / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D012328:RNA Viruses / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D012232:Rhizoctonia / Q000821:virology*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D018076:DNA, Complementary; D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase