title: Engineering bispecific antibodies with defined chain pairing.
abstract: Bispecific IgG-like antibodies can simultaneously interact with two epitopes on the same or on different antigens. Therefore, these molecules facilitate novel modes of action, which cannot be addressed by conventional monospecific IgGs. However, the generation of such antibodies still appears to be demanding due to their specific architecture comprising four different polypeptide chains that need to assemble correctly. This review focusses on different strategies to circumvent this issue or to enforce a correct chain association with a focus on common-chain bispecific antibodies.
authors.affiliation: Institute for Organic Chemistry and Biochemistry, Technische Universität Darmstadt, Alarich-Weiss-Strasse 4, D-64287 Darmstadt, Germany; Protein Engineering and Antibody Technologies, Merck KGaA, Frankfurter Strasse 250, D-64293 Darmstadt, Germany.
authors.forename: Simon
authors.initials: S
authors.lastname: Krah
authors.affiliation: Institute for Organic Chemistry and Biochemistry, Technische Universität Darmstadt, Alarich-Weiss-Strasse 4, D-64287 Darmstadt, Germany; Protein Engineering and Antibody Technologies, Merck KGaA, Frankfurter Strasse 250, D-64293 Darmstadt, Germany.
authors.forename: Carolin
authors.initials: C
authors.lastname: Sellmann
authors.affiliation: Protein Engineering and Antibody Technologies, Merck KGaA, Frankfurter Strasse 250, D-64293 Darmstadt, Germany.
authors.forename: Laura
authors.initials: L
authors.lastname: Rhiel
authors.affiliation: Institute for Organic Chemistry and Biochemistry, Technische Universität Darmstadt, Alarich-Weiss-Strasse 4, D-64287 Darmstadt, Germany; Protein Engineering and Antibody Technologies, Merck KGaA, Frankfurter Strasse 250, D-64293 Darmstadt, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Schröter
authors.affiliation: Institute for Organic Chemistry and Biochemistry, Technische Universität Darmstadt, Alarich-Weiss-Strasse 4, D-64287 Darmstadt, Germany; Protein Engineering and Antibody Technologies, Merck KGaA, Frankfurter Strasse 250, D-64293 Darmstadt, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Dickgiesser
authors.affiliation: Institute for Organic Chemistry and Biochemistry, Technische Universität Darmstadt, Alarich-Weiss-Strasse 4, D-64287 Darmstadt, Germany; Protein Engineering and Antibody Technologies, Merck KGaA, Frankfurter Strasse 250, D-64293 Darmstadt, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Beck
authors.affiliation: Protein Engineering and Antibody Technologies, Merck KGaA, Frankfurter Strasse 250, D-64293 Darmstadt, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Zielonka
authors.affiliation: Protein Engineering and Antibody Technologies, Merck KGaA, Frankfurter Strasse 250, D-64293 Darmstadt, Germany.
authors.forename: Lars
authors.initials: L
authors.lastname: Toleikis
authors.affiliation: Protein Engineering and Antibody Technologies, Merck KGaA, Frankfurter Strasse 250, D-64293 Darmstadt, Germany.
authors.forename: Björn
authors.initials: B
authors.lastname: Hock
authors.affiliation: Institute for Organic Chemistry and Biochemistry, Technische Universität Darmstadt, Alarich-Weiss-Strasse 4, D-64287 Darmstadt, Germany. Electronic address: Kolmar@Biochemie-TUD.de.
authors.forename: Harald
authors.initials: H
authors.lastname: Kolmar
authors.affiliation: Protein Engineering and Antibody Technologies, Merck KGaA, Frankfurter Strasse 250, D-64293 Darmstadt, Germany. Electronic address: Stefan.c.Becker@merckgroup.com.
authors.forename: Stefan
authors.initials: S
authors.lastname: Becker
mesh_terms: D000818:Animals; D018033:Antibodies, Bispecific / Q000378:metabolism*; D006801:Humans; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods*; D055503:Protein Multimerization
chemical_list: D018033:Antibodies, Bispecific; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains
keywords: Antibody; Bispecific antibody; Common chain bispecific antibodies in clinical development; Common heavy chain; Common light chain; Ensuring cognate light chain pairing; Heavy chain heterodimerization