title: Retrieving Quantitative Information of Histone PTMs by Mass Spectrometry.
abstract: Posttranslational modifications (PTMs) of histones are one of the main research interests in the rapidly growing field of epigenetics. Accurate and precise quantification of these highly complex histone PTMs is critical for understanding the histone code and the biological significance behind it. It nonetheless remains a major analytical challenge. Mass spectrometry (MS) has been proven as a robust tool in retrieving quantitative information of histone PTMs, and a variety of MS-based quantitative strategies have been successfully developed and employed in basic research as well as clinical studies. In this chapter, we provide an overview for quantitative analysis of histone PTMs, often highly flexible and case dependent, as a primer for future experimental designs.
authors.affiliation: Baylor College of Medicine, Houston, TX, United States. Electronic address: chunchaz@bcm.edu.
authors.forename: C
authors.initials: C
authors.lastname: Zhang
authors.affiliation: University of Michigan, Ann Arbor, MI, United States. Electronic address: yifan@med.umich.edu.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D056148:Chromatography, Reverse-Phase / Q000592:standards; D006657:Histones / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D011499:Protein Processing, Post-Translational; D059748:Proteolysis; D012015:Reference Standards; D013194:Staining and Labeling; D053719:Tandem Mass Spectrometry / Q000592:standards
chemical_list: D006657:Histones; D010446:Peptide Fragments
keywords: Histone PTM; Label-free quantification; Mass spectrometry; PTM quantification; Stable isotope labeling