title: Gold nanorods reflectance discriminate benign from malignant oral lesions.
abstract: Nanoparticle-based contrast agents have been used as an imaging tool for selectively detecting cancerous processes. We aimed to evaluate the detection sensitivity of reflection measurements of gold nanorods (GNRs) bio-conjugated to anti-epidermal growth factor receptor (GNRs-EGFR) monoclonal antibodies in discriminating benign from premalignant and malignant human oral lesions. Tissue sections incubated with GNRs-EGFR and the reflectance spectrum was measured using hyperspectral microscopy. Reflectance intensity increased with the progression of the disease, lowest in the control group and increasing as the dysplastic changes increase (P<0.001 for linear trend of grade). Intensity was significantly higher in the moderate and severe dysplasias and cancer patients than in the controls and mild dysplasia (t test P=0.0003, Mann-Whitney P<0.0001). The GNRs reflection measurements can discriminate benign and mild dysplastic lesions from the more severe dysplasia and invasive cancer, suggesting an objective, not dependent on the qualification of a technician and with less interpretation errors.
authors.affiliation: Department of Oral Pathology and Oral Medicine, The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel. Electronic address: hirshmd@post.tau.ac.il.
authors.forename: Abraham
authors.initials: A
authors.lastname: Hirshberg
authors.affiliation: Department of Oral Pathology and Oral Medicine, The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Irit
authors.initials: I
authors.lastname: Allon
authors.affiliation: Biostatistical Unit, Gertner institute for Epidemiology and Health Policy Research, Ramat Gan, Israel.
authors.forename: Ilya
authors.initials: I
authors.lastname: Novikov
authors.affiliation: Faculty of Engineering and Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, Israel.
authors.forename: Rinat
authors.initials: R
authors.lastname: Ankri
authors.affiliation: Faculty of Engineering and Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, Israel.
authors.forename: Ariel
authors.initials: A
authors.lastname: Ashkenazy
authors.affiliation: Faculty of Engineering and Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, Israel.
authors.forename: Dror
authors.initials: D
authors.lastname: Fixler
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis*; D066246:ErbB Receptors / Q000037:antagonists & inhibitors; D005260:Female; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000175:diagnosis*; D043942:Nanotubes / Q000737:chemistry*; D055815:Young Adult
chemical_list: D000911:Antibodies, Monoclonal; D006046:Gold; D066246:ErbB Receptors
keywords: Gold nanorods; Mouth neoplasms; Nanoconjugates; Nanospheres; Oral cancer; Oral potentially malignant disorders