title: Antimelanogenic, Antioxidant and Antiproliferative Effects of Antrodia camphorata Fruiting Bodies on B16-F0 Melanoma Cells.
abstract: Antrodia camphorata is a fungus that is endemic to Taiwan, and its fruiting body has been used as a folk medicine for the prevention or treatment of diverse diseases. The present study is aimed at investigating the antimelanogenesis and antioxidation effect of the ethanolic extract of Antrodia camphorata fruiting body (EE-AC), as well as its antiproliferation effects in B16-F0 melanoma cells. Regarding antimelanogenic effects, EE-AC had effective cupric ions reducing capacity and expressed more potent inhibitory effect than kojic acid on mushroom tyrosinase activity. Moreover, EE-AC significantly inhibited cellular tyrosinase activity and the melanin content in B16-F0 cells at 12.5 μg/mL concentration without cell toxicities. Regarding antioxidant effects, EE-AC exhibited potent DPPH radical- and SOD-like-scavenging activities. Regarding antiproliferative effects, EE-AC exhibited a selective cytotoxic effect and markedly inhibited the migration ability of B16-F0 cells. EE-AC increased the population of B16-F0 cells at sub-G1 phase of the cell cycle. EE-AC also caused the increase of early apoptotic cells and chromatin condensation, which indicated the apoptotic effects in B16-F0 cells. We demonstrated that EE-AC possessed antimelanogenic, antioxidant and anti-skin cancer actions. The results would contribute to the development and application of cosmetics, healthy food and pharmaceuticals.
authors.affiliation: Department of Nutrition and Health Science, Fooyin University, Kaohsiung, Taiwan.
authors.forename: Jyh-Jye
authors.initials: JJ
authors.lastname: Wang
authors.affiliation: Department of Nutrition and Health Sciences, Chang Jung Christian University, Tainan, Taiwan.
authors.forename: Chih-Chung
authors.initials: CC
authors.lastname: Wu
authors.affiliation: Department of Life Science, National Taitung University, Taitung, Taiwan.
authors.forename: Chun-Lin
authors.initials: CL
authors.lastname: Lee
authors.affiliation: Department of Seafood Sciences, National Kaohsiung Marine University, Kaohsiung, Taiwan.
authors.forename: Shu-Ling
authors.initials: SL
authors.lastname: Hsieh
authors.affiliation: Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan.
authors.forename: Jin-Bor
authors.initials: JB
authors.lastname: Chen
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, Fooyin University, Kaohsiung, Taiwan.
authors.forename: Chu-I
authors.initials: CI
authors.lastname: Lee
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D055491:Antrodia; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D048690:Fruiting Bodies, Fungal; D008545:Melanoma / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000975:Antioxidants; D010936:Plant Extracts