title: Immune-enrichment of insulin in bio-fluids on gold-nanoparticle decorated target plate and in situ detection by MALDI MS.
abstract: BACKGROUND
  Detection of low-abundance biomarkers using mass spectrometry (MS) is often hampered by non-target molecules in biological fluids. In addition, current procedures for sample preparation increase sample consumption and limit analysis throughput. Here, a simple strategy is proposed to construct an antibody-modified target plate for high-sensitivity MS detection of target markers such as insulin, in biological fluids.
  
  
  METHODS
  The target plate was first modified with gold nanoparticle, and then functionalized with corresponding antibody through chemical conjugation. Clinical specimens were incubated onto these antibody-functionalized target plates, and then subjected to matrix assisted laser desorption ionization mass spectrometry analysis.
  
  
  RESULTS
  Insulin in samples was enriched specifically on this functional plate. The detection just required low-volume samples (lower than 5 µL) and simplified handling process (within 40 min). This method exhibited high sensitivity (limit of detection in standard samples, 0.8 nM) and good linear correlation of MS intensity with insulin concentration (R2= 0.994). More importantly, insulin present in real biological fluids such as human serum and cell lysate could be detected directly by using this functional target plate without additional sample preparations.
  
  
  CONCLUSIONS
  Our method is easy to manipulate, cost-effective, and with a potential to be applied in the field of clinical biomarker detection.
authors.affiliation: Laboratory of Interdisciplinary Research, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101 China.
authors.forename: Kai
authors.initials: K
authors.lastname: Liang
authors.affiliation: Laboratory of Interdisciplinary Research, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101 China.|GuangDong Bio-healtech Advanced Co., Ltd, Foshan City, 52800 GuangDong Province China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Wu
authors.affiliation: Laboratory of Interdisciplinary Research, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101 China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
keywords: Antibody-enrichment; Chemical conjugation; Insulin; MALDI MS