title: Variety of RNAs in Peripheral Blood Cells, Plasma, and Plasma Fractions.
abstract: Human peripheral blood contains RNA in cells and in extracellular membrane vesicles, microvesicles and exosomes, as well as in cell-free ribonucleoproteins. Circulating mRNAs and noncoding RNAs, being internalized, possess the ability to modulate vital processes in recipient cells. In this study, with SOLiD sequencing technology, we performed identification, classification, and quantification of RNAs from blood fractions: cells, plasma, plasma vesicles pelleted at 16,000gand 160,000g, and vesicle-depleted plasma supernatant of healthy donors and non-small cell lung cancer (NSCLC) patients. It was determined that 16,000gblood plasma vesicles were enriched with cell-free mitochondria and with a set of mitochondrial RNAs. The variable RNA set of blood plasma 160,000gpellets reflected the prominent contribution of U1, U5, and U6 small nuclear RNAs' fragments and at the same time was characterized by a remarkable depletion of small nucleolar RNAs. Besides microRNAs, the variety of fragments of mRNAs and snoRNAs dominated in the set of circulating RNAs differentially expressed in blood fractions of NSCLC patients. Taken together, our data emphasize that not only extracellular microRNAs but also circulating fragments of messenger and small nuclear/nucleolar RNAs represent prominent classes of circulating regulatory ncRNAs as well as promising circulating biomarkers for the development of disease diagnostic approaches.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Lavrentiev Avenue 8, Novosibirsk 630090, Russia.
authors.forename: Anna V
authors.identifier: 0000-0002-9572-8904
authors.initials: AV
authors.lastname: Savelyeva
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Lavrentiev Avenue 8, Novosibirsk 630090, Russia.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Kuligina
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Lavrentiev Avenue 8, Novosibirsk 630090, Russia.
authors.forename: Dmitry N
authors.initials: DN
authors.lastname: Bariakin
authors.affiliation: Novosibirsk Regional Clinical Oncology Dispensary, Plahotnogo Street 2, Novosibirsk 630108, Russia.
authors.forename: Vadim V
authors.initials: VV
authors.lastname: Kozlov
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Lavrentiev Avenue 8, Novosibirsk 630090, Russia.
authors.forename: Elena I
authors.identifier: 0000-0003-4714-1524
authors.initials: EI
authors.lastname: Ryabchikova
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Lavrentiev Avenue 8, Novosibirsk 630090, Russia.
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Richter
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Lavrentiev Avenue 8, Novosibirsk 630090, Russia.
authors.forename: Dmitry V
authors.initials: DV
authors.lastname: Semenov
mesh_terms: D000230:Adenocarcinoma / Q000097:blood / Q000235:genetics; D000077192:Adenocarcinoma of Lung; D001773:Blood Cells / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000235:genetics; D016022:Case-Control Studies; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000235:genetics; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics; D008875:Middle Aged; D010949:Plasma / Q000378:metabolism; D012313:RNA / Q000097:blood* / Q000145:classification / Q000235:genetics; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D000077278:RNA, Mitochondrial; D015138:RNA, Nuclear / Q000097:blood / Q000235:genetics
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger; D000077278:RNA, Mitochondrial; D015138:RNA, Nuclear; D012313:RNA