title: Structural Alteration in Dermal Vessels and Collagen Bundles following Exposure of Skin Wound to Zeolite-Bentonite Compound.
abstract: Background. This study examines the impact of one-time direct application of haemostatic agent zeolite-bentonite powder to wounded skin on the healing process in rats.Materials and Methods. 24 male Sprague-Dawley rats were randomly allocated into two groups (n= 12): (1) the rats whose wounds were washed only with sterile normal saline (NS-treated) and (2) those treated with zeolite-bentonite compound (ZEO-treated). The wound was circular, full-thickness, and 2 cm in diameter. At the end of the 12th day, six animals from each group were randomly selected and terminated. The remaining rats were terminated after 21 days. Just after scarification, skin samples were excised and sent for stereological evaluation.Results. The results showed a significant difference between the two groups regarding the length density of the blood vessels and diameter of the large and small vessels on the 12th day after the wound was inflicted. Besides, volume density of both the dermis and collagen bundles was reduced by 25% in the ZEO-treated rats in comparison to the NS-treated animals after 21 days.Conclusions. One-time topical usage of zeolite-bentonite haemostatic powder on an animal skin wound might negatively affect the healing process through vasoconstriction and inhibition of neoangiogenesis.
authors.affiliation: Trauma Research Center, Shahid Rajaee (Emtiaz) Trauma Hospital, Shiraz University of Medical Sciences, Shiraz, Iran; Department of General Surgery, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Shahram
authors.initials: S
authors.lastname: Paydar
authors.affiliation: Histomorphometry and Stereology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Noorafshan
authors.affiliation: Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Internal Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Behnam
authors.identifier: 0000-0003-4547-0965
authors.initials: B
authors.lastname: Dalfardi
authors.affiliation: International Branch, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Shahram
authors.initials: S
authors.lastname: Jahanabadi
authors.affiliation: Medical Physics Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Ionizing and Non-Ionizing Radiation Protection Research Center (INIRPRC), Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Seyed Mohammad Javad
authors.initials: SM
authors.lastname: Mortazavi
authors.affiliation: Histomorphometry and Stereology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Seyedeh-Saeedeh
authors.initials: SS
authors.lastname: Yahyavi
authors.affiliation: Trauma Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Hadi
authors.initials: H
authors.lastname: Khoshmohabat