title: Characterization of glial-restricted precursors from rhesus monkey embryonic stem cells.
abstract: Glial-restricted precursor (GRP) cells, the earliest glial progenitors for both astrocytes and oligodendrocytes, have been derived from embryos and embryonic stem cells (ESC) in rodents. However, knowledge regarding the equivalent cell type in primates is limited due to restrictions imposed by ethics and resources. Here we report successful derivation and characterization of primate GRP cells from rhesus monkey ESC. The purified monkey GRP cells were A2B5-positive and FGF2-dependent for survival and proliferation. The differentiation assays indicated that they were tri-potentialin vitroand bi-potentialin vivo. These newly purified GRP cells will help to facilitate understanding of the molecular mechanism of glial development in primates as well as provide a source of therapeutic donor cells for use in neuroregenerative medicine.
authors.affiliation: Laboratory of Reproductive and Developmental Biology, Kunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P. R. China; Yunnan Key Laboratory of Animal Reproductive Biology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P. R. China; Stem Cell and Brain Research Institute, INSERM U846, Bron 69675, France; Graduate University of Chinese Academy of Sciences, Beijing 100049, P. R. China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Chen
authors.affiliation: Laboratory of Primate Recognition Neurosciences, Kunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P. R. China; Graduate University of Chinese Academy of Sciences, Beijing 100049, P. R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Laboratory of Reproductive and Developmental Biology, Kunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P. R. China; Yunnan Key Laboratory of Animal Reproductive Biology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P. R. China; Graduate University of Chinese Academy of Sciences, Beijing 100049, P. R. China.
authors.forename: Shufen
authors.initials: S
authors.lastname: Wang
authors.affiliation: Laboratory of Reproductive and Developmental Biology, Kunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P. R. China; Yunnan Key Laboratory of Animal Reproductive Biology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P. R. China; Graduate University of Chinese Academy of Sciences, Beijing 100049, P. R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P. R. China.
authors.forename: Jinhuan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Central Laboratory, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P. R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: Laboratory of Primate Recognition Neurosciences, Kunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P. R. China.
authors.forename: Yuanye
authors.initials: Y
authors.lastname: Ma
keywords: Differentiation; Embryonic stem cell (ESC); Glial-restricted precursor (GRP); Rhesus monkey