title: Toxicity and biodegradation of ibuprofen by Bacillus thuringiensis B1(2015b).
abstract: In recent years, the increased intake of ibuprofen has resulted in the presence of the drug in the environment. This work presents results of a study on degradation of ibuprofen at 25 mg L-1in the presence of glucose, as an additional carbon source by Bacillus thuringiensis B1(2015b). In the cometabolic system, the maximum specific growth rate of the bacterial strain was 0.07 ± 0.01 mg mL-1h-1and Ksμ0.27 ± 0.15 mg L-1. The maximum specific ibuprofen removal rate and the value of the half-saturation constant were qmax= 0.24 ± 0.02 mg mL-1h-1and Ks= 2.12 ± 0.56 mg L-1, respectively. It has been suggested that monooxygenase and catechol 1,2-dioxygenase are involved in ibuprofen degradation by B. thuringiensis B1(2015b). Toxicity studies showed that B. thuringiensis B1(2015b) is more resistant to ibuprofen than other tested organisms. The EC50 of ibuprofen on the B1 strain is 809.3 mg L-1, and it is 1.5 times higher than the value of the microbial toxic concentration (MTCavg). The obtained results indicate that B. thuringiensis B1(2015b) could be a useful tool in biodegradation/bioremediation processes.
authors.affiliation: Department of Biochemistry, Faculty of Biology and Environmental Protection, University of Silesia in Katowice, Jagiellońska 28, 40-032, Katowice, Poland.
authors.forename: Ariel
authors.initials: A
authors.lastname: Marchlewicz
authors.affiliation: Department of Biochemistry, Faculty of Biology and Environmental Protection, University of Silesia in Katowice, Jagiellońska 28, 40-032, Katowice, Poland.
authors.forename: Urszula
authors.initials: U
authors.lastname: Guzik
authors.affiliation: Department of Biochemistry, Faculty of Biology and Environmental Protection, University of Silesia in Katowice, Jagiellońska 28, 40-032, Katowice, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Hupert-Kocurek
authors.affiliation: Department of Biochemistry, Faculty of Biology and Environmental Protection, University of Silesia in Katowice, Jagiellońska 28, 40-032, Katowice, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Nowak
authors.affiliation: Department of Biochemistry, Faculty of Biology and Environmental Protection, University of Silesia in Katowice, Jagiellońska 28, 40-032, Katowice, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Wilczyńska
authors.affiliation: Department of Biochemistry, Faculty of Biology and Environmental Protection, University of Silesia in Katowice, Jagiellońska 28, 40-032, Katowice, Poland. danuta.wojcieszynska@us.edu.pl.
authors.forename: Danuta
authors.initials: D
authors.lastname: Wojcieszyńska
mesh_terms: D001413:Bacillus thuringiensis / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004785:Environmental Pollutants / Q000032:analysis / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D007052:Ibuprofen / Q000032:analysis / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity
chemical_list: D004785:Environmental Pollutants; D007052:Ibuprofen
keywords: Bacillus; Biodegradation; Biotransformation; Cometabolism; Ibuprofen; Toxicity