title: Klyflaccicembranols A-I, New Cembranoids from the Soft Coral Klyxum flaccidum.
abstract: New cembranoids klyflaccicembranols A-I (1-9), along with gibberosene D (10), have been isolated from the organic extract of a Formosan soft coralKlyxum flaccidum. Their structures were established by extensive spectroscopic analyses, including 2D NMR spectroscopy, and spectral data comparison with related structures. The cytotoxicity of the isolated metabolites, as well as their nitric oxide (NO) inhibitory activity, were evaluated and reported. Metabolites2,4,6,8and9were found to exhibit variable activities against a limited panel of cancer cell lines in a range of IC5016.5-49.4 μM. Among the tested cembranoids, compounds4,5,6, and9significantly inhibited NO production in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages at a dose of 50 μg/mL.
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. afahmed@ksu.edu.sa.|Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia. afahmed@ksu.edu.sa.|Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt. afahmed@ksu.edu.sa.
authors.forename: Atallah F
authors.initials: AF
authors.lastname: Ahmed
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. qse456awd@gmail.com.
authors.forename: Chia-Ruei
authors.initials: CR
authors.lastname: Tsai
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. huangcy@mail.nsysu.edu.tw.
authors.forename: Chiung-Yao
authors.initials: CY
authors.lastname: Huang
authors.affiliation: Department of Forestry, National Chung Hsing University, Taichung 40227, Taiwan. taiwanfir@dragon.nchu.edu.tw.
authors.forename: Sheng-Yang
authors.initials: SY
authors.lastname: Wang
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. sheu@mail.nsysu.edu.tw.|Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 80756, Taiwan. sheu@mail.nsysu.edu.tw.|Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 40402, Taiwan. sheu@mail.nsysu.edu.tw.|Frontier Center for Ocean Science and Technology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. sheu@mail.nsysu.edu.tw.|Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. sheu@mail.nsysu.edu.tw.
authors.forename: Jyh-Horng
authors.initials: JH
authors.lastname: Sheu
mesh_terms: D000072283:A549 Cells; D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D001688:Biological Products / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D003031:Coal; D004354:Drug Screening Assays, Antitumor; D019073:HT29 Cells; D006801:Humans; D020014:K562 Cells; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism
chemical_list: D001688:Biological Products; D003031:Coal; D008070:Lipopolysaccharides; D009569:Nitric Oxide
keywords: Klyxum flaccidum; NO inhibition; cembranoid; cytotoxicity; soft coral