title: MicroRNA analysis in mouse neuro-2a cells after pseudorabies virus infection.
abstract: Pseudorabies virus (PRV), an alpha herpesvirus can enter the mammalian nervous system, causing Aujezsky's disease. Previous studies have reported an alteration of microRNA (miRNA) expression levels during PRV infections. However, knowledge regarding miRNA response in nervous cells to PRV infection is still unknown. To address this issue, small RNA libraries from infected and uninfected mouse neuroblastoma cells were assessed after Illumina deep sequencing. A total of eight viral miRNA were identified, and ten host miRNAs showed significantly different expression upon PRV infection. Among these, five were analyzed by stem-loop RT-qPCR, which confirmed the above data. Interestingly, these viral miRNAs were mainly found in the large latency transcript region of PRV, and predicted to target a variety of genes, forming a complicated regulatory network. Moreover, ten cellular miRNAs were expressed differently upon PRV infection, including nine upregulated and one downregulated miRNAs. Host targets of these miRNAs obtained by bioinformatics analysis belonged to large signaling networks, mainly encompassing calcium signaling pathway, cAMP signaling pathway, MAPK signaling pathway, and other nervous-associated pathways. These findings further highlighted miRNA features in nervous cells after PRV infection and contributed to unveil the underlying mechanisms of neurotropism as well as the neuropathogenesis of PRV.
authors.affiliation: College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou, 450002, China.
authors.forename: Yongtao
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou, 450002, China.
authors.forename: Guanmin
authors.initials: G
authors.lastname: Zheng
authors.affiliation: College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou, 450002, China.
authors.forename: Yujuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou, 450002, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Yang
authors.affiliation: College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou, 450002, China.
authors.forename: Hongying
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou, 450002, China.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Chang
authors.affiliation: College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou, 450002, China.
authors.forename: Xinwei
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou, 450002, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou, 450002, China.
authors.forename: Chuanqing
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou, 450002, China. chenluhau@sina.com.
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D020013:Calcium Signaling / Q000235:genetics; D045744:Cell Line, Tumor; D000242:Cyclic AMP / Q000378:metabolism; D015723:Gene Library; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D011558:Herpesvirus 1, Suid / Q000235:genetics* / Q000378:metabolism; D059014:High-Throughput Nucleotide Sequencing; D054884:Host-Pathogen Interactions; D020935:MAP Kinase Signaling System / Q000235:genetics; D051379:Mice; D035683:MicroRNAs / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D058977:Molecular Sequence Annotation; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000378:metabolism / Q000821:virology*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D000242:Cyclic AMP
keywords: Aujezsky’s disease; MiRNA; Neuro-2a; Pseudorabies virus