title: Identification of CD4+T-cell-derived CD161+CD39+and CD39+CD73+microparticles as new biomarkers for rheumatoid arthritis.
abstract: OBJECTIVE
  This study aimed to identify CD4+T-cell-derived microparticles (MPs) and investigate their roles in rheumatoid arthritis (RA).
  
  
  METHODS
  Synovial fluids from 34 RA, 33 osteoarthritis patients and 42 healthy individuals were analyzed by flow cytometry. Human fibroblast-like synoviocytes and peripheral blood mononuclear cells were cultured with or without isolated MPs, chemokines and cytokines were measured by ELISA.
  
  
  RESULTS
  CD4+CD161+CD39+and CD4+CD39+CD73+MPs were abundantly present in RA patients, which were positively or negatively correlated with RA features, respectively. Chemokines CCL20, CCL17 and CCL22, and cytokines IL-17 and IL-10 were influenced by these MPs in human fibroblast-like synoviocytes (HFLS) or PMBCs.
  
  
  CONCLUSIONS
  CD4+T-cell-derived CD161+CD39+and CD39+CD73+MPs could serve as new reciprocal biomarkers for RA evaluation.
authors.affiliation: Department of Rheumatology, The Central Hospital of Xinxiang, Xinxiang, Henan, China.
authors.forename: Wenqiang
authors.initials: W
authors.lastname: Fan
authors.affiliation: Department of Rheumatology, The Central Hospital of Xinxiang, Xinxiang, Henan, China.|Department of Gynecology Oncology, The Central Hospital of Xinxiang, Xinxiang, Henan, China.
authors.forename: Wenxiang
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Rheumatology, The Central Hospital of Xinxiang, Xinxiang, Henan, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Rheumatology, The Central Hospital of Xinxiang, Xinxiang, Henan, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Rheumatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Jinyan
authors.initials: J
authors.lastname: Guo
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism; D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D001081:Apyrase / Q000378:metabolism; D001172:Arthritis, Rheumatoid / Q000175:diagnosis*; D015415:Biomarkers / Q000032:analysis*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000378:metabolism*; D016022:Case-Control Studies; D055252:Cell-Derived Microparticles / Q000378:metabolism*; D018925:Chemokines / Q000032:analysis; D016207:Cytokines / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D008875:Middle Aged; D055651:NK Cell Lectin-Like Receptor Subfamily B / Q000378:metabolism; D010003:Osteoarthritis / Q000175:diagnosis* / Q000473:pathology; D013582:Synovial Fluid / Q000166:cytology
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D018925:Chemokines; D016207:Cytokines; D055651:NK Cell Lectin-Like Receptor Subfamily B; D015720:5'-Nucleotidase; D001081:Apyrase; C067869:CD39 antigen
keywords: CD4+ T; T helper 17 cells; Treg; biomarker; fibroblast-like synoviocytes; microparticles; osteoarthritis; peripheral blood mononuclear cells; rheumatoid arthritis; synovial fluid