title: Parahaliea aestuarii sp. nov., isolated from the Asan Bay estuary.
abstract: A Gram-stain-negative, strictly aerobic and moderate halotolerant bacterial strain, designated S2-26T, was isolated from sediment of the Asan Bay estuary in South Korea. Cells were motile rods with two polar flagella showing oxidase and catalase activities. Growth of S2-26T was observed at 15-45 °C (optimum, 25 °C) and pH 5.5-10.0 (optimum, pH 7.0-8.5) and in the presence of 0-8.0 % (w/v) NaCl (optimum, 2.0 %). S2-26T contained C17 : 1ω8c, summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c), summed feature 3 (comprising C16:1ω7c/C16:1ω6c) and C17 : 0 as the major fatty acids and ubiquinone-8 as the sole isoprenoid quinone. The polar lipids of S2-26T consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unknown phospholipid, an unknown aminolipid, an unknown glycolipid and an unknown lipid. The G+C content of the genomic DNA was 62.2 mol%. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that S2-26T formed a tight phylogenetic lineage with Parahaliea mediterranea 7SM29T with a 100 % bootstrap value. S2-26T was most closely related to the type strain of Parahaliea mediterranea, with a 97.8 % 16S rRNA gene sequence similarity, and its DNA-DNA relatedness level was 45.2±2.2 %. On the basis of phenotypic, chemotaxonomic and molecular properties, it is clear that S2-26T represents a novel species of the genus Parahaliea, for which the name Parahaliea aestuarii sp. nov. is proposed. The type strain is S2-26T (=KACC 18801T=JCM 31547T).
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
authors.forename: Hye Su
authors.initials: HS
authors.lastname: Jung
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
authors.forename: Sang Eun
authors.initials: SE
authors.lastname: Jeong
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
authors.forename: Kyung Hyun
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
authors.forename: Che Ok
authors.initials: CO
authors.lastname: Jeon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D061006:Bays; D004269:DNA, Bacterial / Q000235:genetics; D063366:Estuaries; D005227:Fatty Acids / Q000737:chemistry; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019015:Geologic Sediments / Q000382:microbiology*; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8