title: Treatment effects of lysozyme-shelled microbubbles and ultrasound in inflammatory skin disease.
abstract: Acne vulgaris is the most common skin disorder, and is caused by Propionibacterium acnes (P. acnes) and can induce inflammation. Antibiotic therapy often needs to be administered for long durations in acne therapy, which results in extensive antibiotic exposure. The present study investigated a new treatment model for evaluating the antibacterial effects of lysozyme (LY)-shelled microbubbles (MBs) and ultrasound (US)-mediated LY-shelled MBs cavitation against P. acnes both in vitro and in vivo, with the aims of reducing the dose and treatment duration and improving the prognosis of acne vulgaris. In terms of the in vitro treatment efficacy, the growth of P. acnes was inhibited by 86.08 ± 2.99% in the LY-shelled MBs group and by 57.74 ± 3.09% in the LY solution group. For US power densities of 1, 2, and 3 W/cm2in the LY-shelled MBs group, the growth of P. acnes was inhibited by 95.79 ± 3.30%, 97.99 ± 1.16%, and 98.69 ± 1.13%, respectively. The in vivo results showed that the recovery rate on day 13 was higher in the US group with LY-shelled MBs (97.8 ± 19.8%) than in the LY-shelled MBs group (90.3 ± 23.3%). Our results show that combined treatments of US and LY-shelled MBs can significantly reduce the treatment duration and inhibit P.-acnes-induced inflammatory skin diseases.
authors.affiliation: Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan.|Department of Biomedical Engineering, National Defense Medical Center, Taipei 11490, Taiwan.
authors.forename: Ai-Ho
authors.initials: AH
authors.lastname: Liao
authors.affiliation: Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan.
authors.forename: Chi-Ray
authors.initials: CR
authors.lastname: Hung
authors.affiliation: Graduate Institute of Manufacturing Technology, National Taipei University of Technology, Taipei 10608, Taiwan.
authors.forename: Chieh-Fu
authors.initials: CF
authors.lastname: Lin
authors.affiliation: Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei 11490, Taiwan.
authors.forename: Yi-Chun
authors.initials: YC
authors.lastname: Lin
authors.affiliation: Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei 11490, Taiwan.
authors.forename: Hang-Kang
authors.initials: HK
authors.lastname: Chen
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002645:Chickens; D015169:Colony Count, Microbial; D007249:Inflammation / Q000000981:diagnostic imaging* / Q000473:pathology*; D008813:Mice, Inbred ICR; D008826:Microbial Sensitivity Tests; D045423:Microbubbles; D009113:Muramidase / Q000378:metabolism*; D011425:Propionibacterium acnes / Q000187:drug effects / Q000254:growth & development; D012871:Skin Diseases / Q000000981:diagnostic imaging* / Q000382:microbiology / Q000473:pathology*; D014463:Ultrasonography
chemical_list: D000900:Anti-Bacterial Agents; D009113:Muramidase