title: moxDendra2: an inert photoswitchable protein for oxidizing environments.
abstract: Fluorescent proteins (FPs) that can be optically highlighted enable PALM super-resolution microscopy and pulse-chase experiments of cellular molecules. Most FPs evolved in cytoplasmic environments either in the original source organism or in the cytoplasm of bacteria during the course of optimization for research applications. Consequently, many FPs may fold incorrectly in the chemically distinct environments in subcellular organelles. Here, we describe the first monomeric photoswitchable (from green to bright red) FP adapted for oxidizing environments.
authors.affiliation: Albert Einstein College of Medicine, Department of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
authors.forename: Andrii A
authors.initials: AA
authors.lastname: Kaberniuk
authors.affiliation: Albert Einstein College of Medicine, Department of Biochemistry, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
authors.forename: Nicholas C
authors.initials: NC
authors.lastname: Morano
authors.affiliation: Albert Einstein College of Medicine, Department of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
authors.forename: Vladislav V
authors.initials: VV
authors.lastname: Verkhusha
authors.affiliation: Janelia Research Campus, HHMI, 19700 Helix Drive, Ashburn, VA 20147, USA. snappe@janelia.hhmi.org.
authors.forename: Erik Lee
authors.identifier: http://orcid.org/0000-0001-9482-2272
authors.initials: EL
authors.lastname: Snapp
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D037421:Anthozoa / Q000737:chemistry; D005456:Fluorescent Dyes / Q000032:analysis*; D006056:Golgi Apparatus / Q000737:chemistry / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000032:analysis*; D008856:Microscopy, Fluorescence / Q000379:methods*; D010084:Oxidation-Reduction; D055668:Photochemical Processes; D016415:Sequence Alignment
chemical_list: C583169:Dendra2 protein, Dendronephthya; D005456:Fluorescent Dyes; D008164:Luminescent Proteins