title: Karyological study ofOlolygon tripui(Lourenço, Nascimento and Pires, 2009), (Anura, Hylidae) with comments on chromosomal traits among populations.
abstract: To increase the number of cytogenetic characters used inOlolygon tripuisystematics, we applied some cytogenetic techniques such as Giemsa, C- and NOR-banding, and fluorescencein situhybridization (FISH) with 18S rDNA and repetitive microsatellite DNA probes to the study of four populations from Minas Gerais State (southeastern Brazil). All populations showed 2n = 24 and FN = 48, and chromosomal formula 8m + 10sm + 6st. Nucleolar organizing regions (NORs) were located on chromosome pair 6 in all populations, although in the Tripuí locality additional markings were observed on one homologue of chromosome pair 3. These patterns were partially congruent with results obtained using the 18S rDNA FISH probe. The microsatellites repetitive DNA (GA)15and (CAT)10probes accumulated predominantly in the terminal region of all chromosomes. Chromosome morphology and Ag-NOR were conserved among populations, a conserved pattern inOlolygonFitzinger, 1843. Repetitive DNA FISH probes patterns were similar among populations, but they revealed species-specific differences when compared with other species of the genusOlolygon, suggesting that molecular cytogenetics are potentially more informative in karyologically conservative taxa.
authors.affiliation: Laboratório de Sistemática Molecular - Beagle, Departamento de Biologia Animal, Universidade Federal de Viçosa, CEP 36570-900, Viçosa, Minas Gerais State, Brazil; Museu de Zoologia João Moojen - MZUFV, Departamento de Biologia Animal, Universidade Federal de Viçosa, CEP 36570-900, Viçosa, Minas Gerais State, Brazil.
authors.forename: Marco Antônio A
authors.initials: MA
authors.lastname: Peixoto
authors.affiliation: Laboratório de Sistemática Molecular - Beagle, Departamento de Biologia Animal, Universidade Federal de Viçosa, CEP 36570-900, Viçosa, Minas Gerais State, Brazil.
authors.forename: Marina P C
authors.initials: MP
authors.lastname: Oliveira
authors.affiliation: Museu de Zoologia João Moojen - MZUFV, Departamento de Biologia Animal, Universidade Federal de Viçosa, CEP 36570-900, Viçosa, Minas Gerais State, Brazil.
authors.forename: Renato N
authors.initials: RN
authors.lastname: Feio
authors.affiliation: Laboratório de Sistemática Molecular - Beagle, Departamento de Biologia Animal, Universidade Federal de Viçosa, CEP 36570-900, Viçosa, Minas Gerais State, Brazil.
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Dergam
keywords: 18S rDNA; Ag-NOR; Cytotaxonomy; FISH; heterochromatic blocks; microsatellite DNA probes; population cytogenetics