title: Theoretical and Instrumental Studies of the Competitive Interaction Between Aromatic α-Aminobisphosphonates with DNA Using Binding Probes.
abstract: Fluorescence spectroscopy, UV-visible absorption spectroscopy, circular dichroism (CD) spectroscopy, viscometry, cyclic voltammetry (CV), and differential pulse voltammetry (DPV) were applied to investigate the competitive interaction of DNA with two aromatic α-aminobisphosphonates and neutral red dye (NR, intercalator) and Hoechst (Ho, groove binder) as spectroscopic probes, in a Tris-hydrogen chloride buffer solution (pH 7.4). The principal component analysis (PCA) was applied to determine the number of chemical components presented in complexation equilibrium of DNA with the aromatic α-aminobisphosphonates (B1and B2). The spectroscopic and voltammetric studies showed that the groove binding mode of interaction is predominant in the solution containing DNA and α-aminobisphosphonates. Furthermore, the results indicated that α-aminobisphosphonate with the lengthy N-alkyl chains had a stronger interaction. The PCA and theoretical quantum mechanical and molecular mechanic methods were also utilized to determine the structure of DNA with the two α-aminobisphosphonates (B1and B2).
authors.affiliation: Faculty of Chemistry, Sensor and Biosensor Research Center (SBRC) and Nanoscience and Nanotechnology Research Center (NNRC), Razi University, Kermanshah, Iran.
authors.forename: M B
authors.initials: MB
authors.lastname: Gholivand
authors.affiliation: Department of Chemistry, Ilam Branch, Faculty of Science, Islamic Azad University of Ilam, Ilam, Iran. H.peyman@ilam-iau.ac.ir.
authors.forename: H
authors.initials: H
authors.lastname: Peyman
authors.affiliation: Department of Chemistry, Faculty of Science, Tarbiat Modares University, Tehran, Iran.
authors.forename: Kh
authors.initials: K
authors.lastname: Gholivand
authors.affiliation: Department of Chemistry, Ilam Branch, Faculty of Science, Islamic Azad University of Ilam, Ilam, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Roshanfekr
authors.affiliation: Department of Organic Chemistry, Faculty of Chemistry, Razi University, P.O. Box: 67149-67346, Kermanshah, Iran.|Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: A A
authors.initials: AA
authors.lastname: Taherpour
authors.affiliation: Department of Chemistry, Faculty of Science, Tarbiat Modares University, Tehran, Iran.
authors.forename: R
authors.initials: R
authors.lastname: Yaghobi
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D004164:Diphosphonates / Q000737:chemistry*; D008956:Models, Chemical
chemical_list: D004164:Diphosphonates; D004247:DNA; C427203:calf thymus DNA
keywords: DNA; Groove binding mode; Hoechst; Molecular modeling; Neutral red; Principal component analysis; α-Aminobisphosphonate