title: Polyporus squamosus Lectin 1a (PSL1a) Exhibits Cytotoxicity in Mammalian Cells by Disruption of Focal Adhesions, Inhibition of Protein Synthesis and Induction of Apoptosis.
abstract: PSL1a is a lectin from the mushroom Polyporus squamosus that binds to sialylated glycans and glycoconjugates with high specificity and selectivity. In addition to its N-terminal carbohydrate-binding domain, PSL1a possesses a Ca2+-dependent proteolytic activity in the C-terminal domain. In the present study, we demonstrate that PSL1a has cytotoxic effects on mammalian cancer cells, and we show that the cytotoxicity is dependent on the cysteine protease activity. PSL1a treatment leads to cell rounding and detachment from the substratum, concomitant with disruption of vinculin complexes in focal adhesions. We also demonstrate that PSL1a inhibits protein synthesis and induces apoptosis in HeLa cells, in a time- and concentration-dependent manner.
authors.affiliation: Department of Chemistry, University of Oslo, Oslo, Norway.|Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway.|Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Oslo, Norway.
authors.forename: Dipankar
authors.initials: D
authors.lastname: Manna
authors.affiliation: Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway.|Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Oslo, Norway.
authors.forename: Sascha
authors.initials: S
authors.lastname: Pust
authors.affiliation: Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway.|Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Oslo, Norway.
authors.forename: Maria L
authors.initials: ML
authors.lastname: Torgersen
authors.affiliation: Department of Chemistry, University of Oslo, Oslo, Norway.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Cordara
authors.affiliation: Institute of Microbiology, ETH Zürich, Zürich, Switzerland.
authors.forename: Markus
authors.initials: M
authors.lastname: Künzler
authors.affiliation: Department of Chemistry, University of Oslo, Oslo, Norway.
authors.forename: Ute
authors.initials: U
authors.lastname: Krengel
authors.affiliation: Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway.|Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Oslo, Norway.|Department of Biosciences, Faculty of Mathematics and Natural Sciences, University of Oslo, Oslo, Norway.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Sandvig
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001343:Autophagy / Q000187:drug effects; D045744:Cell Line, Tumor; D003470:Culture Media; D004261:DNA Replication / Q000187:drug effects; D022001:Focal Adhesions / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000494:pharmacology*; D055492:Polyporus / Q000378:metabolism*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D059748:Proteolysis
chemical_list: D003470:Culture Media; D037102:Lectins; D011500:Protein Synthesis Inhibitors