title: Rapid and high-throughput colorimetric screening for anti-aggregation reagents of protein conformational diseases by using gold nanoplasmonic particles.
abstract: Cellular deposition of destabilized proteins and their aggregates is considered one of the most indisputable factors implicated in protein conformational diseases. Here, we report an innovative high-throughput screening method for discovering anti-aggregation reagents out of numerous potential candidates by using gold nanoplasmonic particles. In our method, nanoparticles act as catalytic activators to accelerate protein aggregation and simultaneously exhibit a colorimetric response according to their embedded shape on the protein aggregates. Using this principle, we observed the colorimetric response to the anti-aggregation effect of amyloid β (Aβ) with the naked eye within a few minutes. Investigation of the anti-aggregation effects of select candidates under three different protein aggregation stages showed that the anti-aggregation efficiency could relate to disease progression. Finally, results obtained with spiked samples in cerebrospinal fluid as well as under various denaturation conditions and different Aβ compositions show the feasibility of future personalized medicine considering individual patient's disease progression.
authors.affiliation: Department of Life Science, University of Seoul, Seoul, Republic of Korea.
authors.forename: Hye Young
authors.initials: HY
authors.lastname: Kim
authors.affiliation: Department of Life Science, University of Seoul, Seoul, Republic of Korea.
authors.forename: Jung A
authors.initials: JA
authors.lastname: Kwon
authors.affiliation: Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, Republic of Korea. Electronic address: twkang@sogang.ac.kr.
authors.forename: Taewook
authors.initials: T
authors.lastname: Kang
authors.affiliation: Department of Life Science, University of Seoul, Seoul, Republic of Korea. Electronic address: inheechoi1@uos.ac.kr.
authors.forename: Inhee
authors.initials: I
authors.lastname: Choi
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry*; D003124:Colorimetry; D006046:Gold / Q000737:chemistry*; D057166:High-Throughput Screening Assays; D006801:Humans; D007202:Indicators and Reagents; D053768:Metal Nanoparticles / Q000737:chemistry*; D066263:Protein Aggregation, Pathological / Q000188:drug therapy*; D011487:Protein Conformation
chemical_list: D016229:Amyloid beta-Peptides; D007202:Indicators and Reagents; D006046:Gold
keywords: Anti-aggregation reagent; Colorimetric sensor; Gold nanoplasmonic particle; High-throughput screening; Protein conformational disease