title: Characterization and biocompatibility evaluation of cutaneous formulations containing lipid nanoparticles.
abstract: Nanostructured lipid carriers (NLC) are well-known systems that show effectiveness to improve skin hydration, being suggested for cosmetic and dermatological use. Nonetheless, NLC dispersions present low viscosity, which is non-attractive for cutaneous application. To circumvent this drawback, the dispersions can be gelled or incorporated in semisolid systems, increasing the final formulation consistency. In this study, we prepared a hydrogel based on NLC containing vitamin E (HG-NLCVE) and evaluated its suitability for cutaneous application. The experiments started with the HG-NLCVEcharacterization (organoleptic analysis, accelerated stability, particle size, morphology, pH, texture and rheology). Afterwards, in vitro experiments were carried out, evaluating the formulation biocompatibility (MTT and Neutral Red) and irritant potential (Hen's egg test on the chorioallantoic membrane, HET-CAM) for cutaneous application. The results showed that the HG-NLCVEhas adequate features for skin application, is biocompatible and non-irritant. From this study, it was predicted the in vivo irritant potential of the developed formulation, avoiding the need to perform a high number of tests on human volunteers. Regarding vitamin E and NLC potential to improve skin hydration, we suggest that the HG-NLCVEcould be used in cosmetic (e.g. moisturizers and anti-aging) or dermatologic (e.g. xerosis and other skin disorders) products.
authors.affiliation: UCIBIO, ReQuimTe, Laboratory of Pharmaceutical Technology/Centre of Research in Pharmaceutical Sciences, Faculty of Pharmacy, Porto University, Porto, Portugal.
authors.forename: F
authors.initials: F
authors.lastname: Eiras
authors.affiliation: UCIBIO, ReQuimTe, Laboratory of Pharmaceutical Technology/Centre of Research in Pharmaceutical Sciences, Faculty of Pharmacy, Porto University, Porto, Portugal. Electronic address: hamaral@ff.up.pt.
authors.forename: M H
authors.initials: MH
authors.lastname: Amaral
authors.affiliation: UCIBIO, REQUINTE, Laboratory of Toxicology, Department of Biological Sciences, Faculty of Pharmacy, Porto University, Porto, Portugal.
authors.forename: R
authors.initials: R
authors.lastname: Silva
authors.affiliation: UCIBIO, REQUINTE, Laboratory of Toxicology, Department of Biological Sciences, Faculty of Pharmacy, Porto University, Porto, Portugal.
authors.forename: E
authors.initials: E
authors.lastname: Martins
authors.affiliation: UCIBIO, ReQuimTe, Laboratory of Pharmaceutical Technology/Centre of Research in Pharmaceutical Sciences, Faculty of Pharmacy, Porto University, Porto, Portugal.
authors.forename: J M Sousa
authors.initials: JMS
authors.lastname: Lobo
authors.affiliation: UCIBIO, ReQuimTe, Laboratory of Pharmaceutical Technology/Centre of Research in Pharmaceutical Sciences, Faculty of Pharmacy, Porto University, Porto, Portugal; FP-ENAS (UFP Energy, Environment and Health Research Unit), CEBIMED (Biomedical Research Centre), Faculty of Health Sciences, Fernando Pessoa University, Rua Carlos da Maia, 296, P-4200-150 Porto, Portugal. Electronic address: ana.silva@ff.up.pt.
authors.forename: A C
authors.initials: AC
authors.lastname: Silva
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002460:Cell Line; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002645:Chickens; D003358:Cosmetics / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D005782:Gels / Q000737:chemistry; D006801:Humans; D007509:Irritants / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D012212:Rheology; D012867:Skin / Q000378:metabolism*; D012869:Skin Absorption / Q000187:drug effects*; D014783:Viscosity; D014810:Vitamin E / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D003358:Cosmetics; D004337:Drug Carriers; D005782:Gels; D007509:Irritants; D008055:Lipids; D014810:Vitamin E
keywords: Biocompatibility; Cutaneous application; Hydrogel; Irritant potential; Nanostructured lipid carriers; Vitamin E