title: Colonization study of gfp-tagged Achromobacter marplatensis strain in sugar beet.
abstract: This study details the introduction of a gfp marker into an endophytic bacterial strain (Achromobacter marplatensis strain 17, isolated from sugar beet) to monitor its colonization of sugar beet (Beta. vulgaris L.). Stability of the plasmid encoding the gfp was confirmed in vitro for at least 72 h of bacterial growth and after the colonization of tissues, under nonselective conditions. The colonization was observed using fluorescence microscopy and enumeration of culturable endophytes in inoculated sugar beet plants that grew for 10 or 20 days. gfp-Expressing strains were re-isolated from the inner tissues of surface-sterilized roots and stems of inoculated plants, and the survival of the Achromobacter marplatensis 17:gfp strain in plants 20 days after inoculation, even in the absence of selective pressure, suggests that it is good colonizer. These results also suggest that this strain could be a useful tool for the delivery of enzymes or other proteins into plants. In addition, the study highlights that sugar beet plants can be used effectively for detailed in vitro studies on the interactions between A. marplatensis strain 17 and its host, particularly if a gfp-tagged strain of the pathogen is used.
authors.affiliation: Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang, P. R. China.|Xinjiang Laboratory of Special Environmental Microbiology, Urumqi 830091, Xinjiang, P. R. China.
authors.forename: YingWu
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang, P. R. China.|Xinjiang Laboratory of Special Environmental Microbiology, Urumqi 830091, Xinjiang, P. R. China.
authors.forename: Chun
authors.initials: C
authors.lastname: Li
authors.affiliation: Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang, P. R. China.|Xinjiang Laboratory of Special Environmental Microbiology, Urumqi 830091, Xinjiang, P. R. China.
authors.forename: HongMei
authors.initials: H
authors.lastname: Yang
authors.affiliation: Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang, P. R. China.|Xinjiang Laboratory of Special Environmental Microbiology, Urumqi 830091, Xinjiang, P. R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang, P. R. China.|Xinjiang Laboratory of Special Environmental Microbiology, Urumqi 830091, Xinjiang, P. R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang, P. R. China.|Xinjiang Laboratory of Special Environmental Microbiology, Urumqi 830091, Xinjiang, P. R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Chu
authors.affiliation: Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang, P. R. China.|Xinjiang Laboratory of Special Environmental Microbiology, Urumqi 830091, Xinjiang, P. R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zeng
authors.affiliation: Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang, P. R. China.|Xinjiang Laboratory of Special Environmental Microbiology, Urumqi 830091, Xinjiang, P. R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Lin
authors.affiliation: Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang, P. R. China.|Xinjiang Laboratory of Special Environmental Microbiology, Urumqi 830091, Xinjiang, P. R. China.
authors.lastname: OuTiKuEr
authors.affiliation: Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang, P. R. China.|Xinjiang Laboratory of Special Environmental Microbiology, Urumqi 830091, Xinjiang, P. R. China.
authors.forename: YuGuo
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang, P. R. China.|Xinjiang Laboratory of Special Environmental Microbiology, Urumqi 830091, Xinjiang, P. R. China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Huo
authors.affiliation: Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang, P. R. China. loukai02@mail.tsinghua.edu.cn.|Xinjiang Laboratory of Special Environmental Microbiology, Urumqi 830091, Xinjiang, P. R. China. loukai02@mail.tsinghua.edu.cn.
authors.forename: Kai
authors.initials: K
authors.lastname: Lou
mesh_terms: D042402:Achromobacter / Q000737:chemistry / Q000235:genetics / Q000254:growth & development*; D027461:Beta vulgaris / Q000382:microbiology*; D060026:Endophytes / Q000737:chemistry / Q000235:genetics / Q000254:growth & development*; D042822:Genomic Instability; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D008856:Microscopy, Fluorescence; D018517:Plant Roots / Q000382:microbiology; D018547:Plant Stems / Q000382:microbiology; D010957:Plasmids; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics; D013194:Staining and Labeling
chemical_list: D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins
keywords: colonization; endophytes; green fluorescent protein; sugar beet