title: Comparative transcriptional survey between self-incompatibility and self-compatibility in Citrus reticulata Blanco.
abstract: Seedlessness is an excellent economical trait, and self-incompatibility (SI) is one of important factors resulting in seedless fruit in Citrus. However, SI molecular mechanism in Citrus is still unclear. In this study, RNA-Seq technology was used to identify differentially expressed genes related to SI reaction of 'Wuzishatangju' (Citrus reticulata Blanco). A total of 35.67GB raw RNA-Seq data was generated and was de novo assembled into 50,364 unigenes with an average length of 897bp and N50 value of 1549. Twenty-three candidate unigenes related to SI were analyzed using qPCR at different tissues and stages after self- and cross-pollination. Seven pollen S genes (Unigene0050323, Unigene0001060, Unigene0004230, Unigene0004222, Unigene0012037, Unigene0048889 and Unigene0004272), three pistil S genes (Unigene0019191, Unigene0040115, Unigene0036542) and three genes (Unigene0038751, Unigene0031435 and Unigene0029897) associated with the pathway of ubiquitin-mediated proteolysis were identified. Unigene0031435, Unigene0038751 and Unigene0029897 are probably involved in SI reaction of 'Wuzishatangju' based on expression analyses. The present study provides a new insight into the molecular mechanism of SI in Citrus at the transcriptional level.
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Yuewen
authors.initials: Y
authors.lastname: Ma
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Qiulei
authors.initials: Q
authors.lastname: Li
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Guibing
authors.initials: G
authors.lastname: Hu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, China. Electronic address: qinyh@scau.edu.cn.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Qin
mesh_terms: D002957:Citrus / Q000235:genetics* / Q000502:physiology*; D035264:Flowers / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics*; D011058:Pollen / Q000502:physiology; D018749:RNA, Plant / Q000235:genetics; D060436:Self-Incompatibility in Flowering Plants; D017423:Sequence Analysis, RNA; D059467:Transcriptome
chemical_list: D010940:Plant Proteins; D018749:RNA, Plant
keywords: Citrus reticulata Blanco; F-box; RNA-Seq; S-RNase; SI; U/26S; qPCR