title: Molecular characterization of canine parvovirus in Vientiane, Laos.
abstract: The global emergence of canine parvovirus type 2c (CPV-2c) has been well documented. In the present study, 139 rectal swab samples collected from diarrheic dogs living in Vientiane, Laos, in 2016 were tested for the presence of the canine parvovirus (CPV) VP2 gene by PCR. The results showed that 82.73% (115/139) of dogs were CPV positive by PCR. The partial VP2 gene was sequenced in 94 of the positive samples; 91 samples belonged to CPV-2c (426Glu) subtype, while 3 samples belonged to the CPV-2a (426Asn) subtype. Notably, phylogenetic analysis of amino acid sequences revealed a close relationship between Laotian isolates and novel Chinese CPV-2c isolates. In Laotian CPV isolates, aligned protein sequences indicated a high rate of residue substitutions at positions 305, 324, 345, 370, 375, and 426 in the GH loop. The mutation at residue 370 (Q370R), a single mutation, was characterized as a unique mutant residue specific to the Laotian CPV-2c variant.
authors.affiliation: Master of Science Program in Veterinary Science, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand.
authors.forename: Soulasack
authors.initials: S
authors.lastname: Vannamahaxay
authors.affiliation: Department of Livestock and Fisheries, Faculty of Agriculture, National University of Laos, Vientiane, Laos.
authors.forename: Souliya
authors.initials: S
authors.lastname: Vongkhamchanh
authors.affiliation: Department of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand.
authors.forename: Montira
authors.initials: M
authors.lastname: Intanon
authors.affiliation: Small Animal Clinic, Department of Companion Animal and Wildlife Clinic, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand.
authors.forename: Sahatchai
authors.initials: S
authors.lastname: Tangtrongsup
authors.affiliation: Department of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand.
authors.forename: Saruda
authors.initials: S
authors.lastname: Tiwananthagorn
authors.affiliation: Department of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand.
authors.forename: Kidsadagon
authors.initials: K
authors.lastname: Pringproa
authors.affiliation: Department of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand. phongsakorn.c@cmu.ac.th.
authors.forename: Phongsakorn
authors.initials: P
authors.lastname: Chuammitri
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D004196:Disease Outbreaks / Q000662:veterinary*; D004283:Dog Diseases / Q000453:epidemiology / Q000821:virology*; D004285:Dogs; D005759:Gastroenteritis / Q000662:veterinary* / Q000821:virology*; D014644:Genetic Variation; D007812:Laos / Q000453:epidemiology; D010322:Parvoviridae Infections / Q000453:epidemiology / Q000821:virology*; D017993:Parvovirus, Canine / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral