title: PacMYBA, a sweet cherry R2R3-MYB transcription factor, is a positive regulator of salt stress tolerance and pathogen resistance.
abstract: Plant R2R3-MYB transcription factors play crucial roles in stress responses. We previously isolated a R2R3-MYB homolog from sweet cherry cv. Hong Deng, designated PacMYBA (GenBank accession No. KF974774). To explore the role of PacMYBA in the plant stress response, we heterologously expressed PacMYBA in transgenic Arabidopsis thaliana plants. In a previous study, we demonstrated that PacMYBA is mainly localized to the nucleus and could be induced by abscisic acid (ABA). Analysis of the promoter sequence of PacMYBA revealed that it contains several stress-related cis-elements. QPCR results showed that PacMYBA is induced by salt, salicylic (SA), and jasmonic acid (JA) in sweet cherry leaves. Transgenic Arabidopsis plants heterologously expressing PacMYBA exhibited enhanced salt-tolerance and increased resistance to Pseudomonas syringe pv. tomato (Pst) DC3000 infection. Overexpression of PacMYBA decreased the osmotic potential (OP), increased the free proline content, and increased the peroxidase content in transgenic Arabidopsis plants. Furthermore, overexpression of PacMYBA also affected the expression levels of salt stress- and pathogen defense-related genes in the transgenic plants. These results indicate that PacMYBA is a positive regulator of salt stress tolerance and pathogen resistance.
authors.affiliation: Department of Fruit Science, College of Horticulture, China Agricultural University, 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, People's Republic of China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture of People's Republic of China, Oilcrops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, People's Republic of China.
authors.forename: Xinjie
authors.initials: X
authors.lastname: Shen
authors.affiliation: Department of Fruit Science, College of Horticulture, China Agricultural University, 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, People's Republic of China.
authors.forename: Xinwei
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Fruit Science, College of Horticulture, China Agricultural University, 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Fruit Science, College of Horticulture, China Agricultural University, 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, People's Republic of China.
authors.forename: Di
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture of People's Republic of China, Oilcrops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Daqing Branch, Heilongjiang Academy of Agricultural Sciences, Daqing 163316, Heilongjiang, People's Republic of China.
authors.forename: Jingsheng
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Fruit Science, College of Horticulture, China Agricultural University, 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, People's Republic of China; Beijing Collaborative Innovation Center for Eco-environmental Improvement with Forestry and Fruit Trees, Beijing 102206, People's Republic of China. Electronic address: lith@cau.edu.cn.
authors.forename: Tianhong
authors.initials: T
authors.lastname: Li
mesh_terms: D000085:Acetates / Q000494:pharmacology; D017360:Arabidopsis / Q000235:genetics / Q000382:microbiology; D003517:Cyclopentanes / Q000494:pharmacology; D060467:Disease Resistance / Q000187:drug effects*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D054883:Oxylipins / Q000494:pharmacology; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D000068220:Prunus avium / Q000187:drug effects / Q000235:genetics / Q000382:microbiology* / Q000502:physiology*; D011549:Pseudomonas / Q000502:physiology; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D020156:Salicylic Acid / Q000494:pharmacology; D055049:Salt Tolerance / Q000187:drug effects*; D015398:Signal Transduction / Q000187:drug effects; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects* / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; D014157:Transcription Factors; D012965:Sodium Chloride; C072239:methyl jasmonate; D020156:Salicylic Acid
keywords: Arabidopsis thaliana; Pathogen infection; R2R3-MYB transcription factor; Salt stress; Sweet cherry