title: Ultrasensitive Sensing Material Based on Opal Photonic Crystal for Label-Free Monitoring of Transferrin.
abstract: A new opal photonic crystal (PC) sensing material, allowing label-free detection of transferrin (TRF), is proposed in the current study. This photonic crystal was prepared via a vertical convective self-assembly method with monodisperse microspheres polymerized by methyl methacrylate (MMA) and 3-acrylamidophenylboronic acid (AAPBA). FTIR, TG, and DLS were used to characterize the components and particle size of the monodisperse microspheres. SEM was used to observe the morphology of the PC. The diffraction peak intensity decreases as the TRF concentration increase. This was due to the combination of TRF to the boronic acid group of the photonic crystal. After condition optimization, a standard curve was obtained and the linear range of TRF concentration was from 2 × 10-3ng/mL to 200 ng/mL. Measurement of TRF concentration in simulated urine sample was also investigated using the sensing material. The results indicated that the PC provided a cheap, label-free, and easy-to-use alternative for TRF determination in clinical diagnostics.
authors.affiliation: Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Health and Environmental Medicine , Tianjin 300050, China.|College of Public Health, Inner Mongolia Medical University , Hohhot, 010059, China.
authors.forename: Enqi
authors.identifier: http://orcid.org/0000-0001-9282-5656
authors.initials: E
authors.lastname: Wu
authors.affiliation: Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Health and Environmental Medicine , Tianjin 300050, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Health and Environmental Medicine , Tianjin 300050, China.
authors.forename: Xihao
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Health and Environmental Medicine , Tianjin 300050, China.
authors.forename: Jialei
authors.initials: J
authors.lastname: Bai
authors.affiliation: Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Health and Environmental Medicine , Tianjin 300050, China.
authors.forename: Yanqiu
authors.initials: Y
authors.lastname: Song
authors.affiliation: Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Health and Environmental Medicine , Tianjin 300050, China.
authors.forename: Houluo
authors.initials: H
authors.lastname: He
authors.affiliation: Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Health and Environmental Medicine , Tianjin 300050, China.|College of Public Health, Inner Mongolia Medical University , Hohhot, 010059, China.
authors.forename: Longxing
authors.initials: L
authors.lastname: Fan
authors.affiliation: College of Public Health, Inner Mongolia Medical University , Hohhot, 010059, China.
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Qu
authors.affiliation: Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Health and Environmental Medicine , Tianjin 300050, China.
authors.forename: Zhixian
authors.initials: Z
authors.lastname: Gao
authors.affiliation: College of Public Health, Inner Mongolia Medical University , Hohhot, 010059, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Health and Environmental Medicine , Tianjin 300050, China.|College of Public Health, Inner Mongolia Medical University , Hohhot, 010059, China.
authors.forename: Baoan
authors.initials: B
authors.lastname: Ning
mesh_terms: D008863:Microspheres; D010316:Particle Size; D017785:Photons; D058105:Polymerization; D014168:Transferrin / Q000737:chemistry*
chemical_list: D014168:Transferrin
keywords: label-free detection; opal photonic crystal; sensing material; soap-free emulsion polymerization; transferrin