title: Antioxidant and antiapoptotic effects of erdosteine in a rat model of ovarian ischemia-reperfusion injury.
abstract: OBJECTIVE
  To evaluate the protective effect of erdosteine, an antiapoptotic and antioxidant agent, on torsion-detorsion evoked histopathological changes in experimental ovarian ischemia-reperfusion (IR) injury.
  
  
  METHODS
  Eighteen female Wistar albino rats were used in control, IR, and IR+Edosteine (IR-E) groups, (n=6 in each). The IR-E group received the erdosteine for seven days before the induction of torsion/retorsion, (10 mg/kg/days). The IR and IR-E groups were exposed to right unilateral adnexal torsion for 3 hr. Three hours later, re-laparotomy was performed, and the right ovaries were surgically excised. Oxidant and antioxidants levels were determined in serum. The ovarian tissue samples were received and fixed with 10% neutral buffered formalin. The sections were stained with H&E, anti-PCNA, and TUNEL.
  
  
  RESULTS
  The IR group were showed severe acute inflammation, polynuclear leukocytes and macrophages, stromal oedema and haemorrhage. Treatment with erdosteine in rats significantly retained degenerative changes in the ovary PCNA (+) cell numbers were significantly decreased in the IR and IR-E groups unlike the control group. However, its numbers were significantly increased in the IR-E group unlike the IR group. TUNEL (+) cell numbers were significantly increased in the IR group unlike the control and the IR-E groups. In erdosteine treated group, TUNEL (+) cells were detected significantly less than the IR group (P<0.05).
  
  
  CONCLUSIONS
  In conclusion, erdosteine maybe a protective agent for ovarian damage and decreasing lipid peroxidation products and leukocytes aggregation after adnexal torsion in animals.
authors.affiliation: University of Trakya, Faculty of Medicine, Department of Obstetrics and Gynecology, 22030, Balkan Campus, Edirne, Turkey.
authors.forename: Vedat
authors.initials: V
authors.lastname: Ugurel
authors.affiliation: University of Trakya, Faculty of Medicine, Department of Histology and Embryology, 22030, Balkan Campus, Edirne, Turkey.
authors.forename: Ahmet Cagatay
authors.initials: AC
authors.lastname: Cicek
authors.affiliation: Yildiz Technical University, Biochemistry Division, Department of Bioengineering, Faculty of Chemical and Metallurgical Engineering, Istanbul, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Cemek
authors.affiliation: University of Trakya, Faculty of Medicine, Department of Histology and Embryology, 22030, Balkan Campus, Edirne, Turkey.
authors.forename: Selim
authors.initials: S
authors.lastname: Demirtas
authors.affiliation: Yildiz Technical University, Biochemistry Division, Department of Bioengineering, Faculty of Chemical and Metallurgical Engineering, Istanbul, Turkey.
authors.forename: A Tuba
authors.initials: AT
authors.lastname: Kocaman
authors.affiliation: University of Trakya, Faculty of Medicine, Department of Histology and Embryology, 22030, Balkan Campus, Edirne, Turkey.
authors.forename: Turan
authors.initials: T
authors.lastname: Karaca
keywords: Antioxidant; Erdosteine; Ischemia-reperfusion Oxidant; Ovary; Rat