title: NEU1 sialidase controls gene expression and secretion of IL-6 and MCP-1 through NF-κB pathway in 3T3-L1 adipocytes.
abstract: A sialidase NEU1 that removes sialic acids from glycoconjugates has been implicated in diverse cellular functions. Aberrant NEU1 activity is associated with various pathologies including lysosomal storage disorder sialidosis, autoimmune diseases and the malignancy and metastasis of cancer cells. We recently reported that NEU1 activity increases during 3T3-L1 adipogenesis and that it is higher in the epididymal fat of obese and diabetic mice. However, the precise functions of NEU1 in adipocytes have not been elucidated. Knockdown of NEU1 using siRNA transfection in 3T3-L1 adipocytes significantly decreased the mRNA expression and protein secretion of IL-6 and MCP-1 induced by LPS. The promoter activities of both IL-6 and MCP-1 as well as nuclear factor-kappa B (NF-κB) nuclear translocation were reduced in adipocytes transfected with an siRNA sequence that targets NEU1(siNEU1). NEU1 suppression using siNEU1 affected TLR4 sialylation. These findings suggest that NEU1 is involved in the production of IL-6 and MCP-1 in adipocytes possibly through TLR4/NF-κB signalling.
authors.affiliation: Department of Life and Health Sciences, School of Pharma-Sciences, Teikyo University.
authors.forename: Yujin
authors.initials: Y
authors.lastname: Natori
authors.affiliation: Department of Life and Health Sciences, School of Pharma-Sciences, Teikyo University.
authors.forename: Miwako
authors.initials: M
authors.lastname: Nasui
authors.affiliation: Department of Research Center for Practical Training of Student Pharmacists, School of Pharma-Sciences, Teikyo University, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan.
authors.forename: Kiyoto
authors.initials: K
authors.lastname: Edo
authors.affiliation: Department of Molecular Predictive Medicine and Sport Science, School of Medicine, Kyorin University, Mitaka, Tokyo 181-8611, Japan.
authors.forename: Shogo
authors.initials: S
authors.lastname: Sato
authors.affiliation: Department of Molecular Predictive Medicine and Sport Science, School of Medicine, Kyorin University, Mitaka, Tokyo 181-8611, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Sakurai
authors.affiliation: Department of Molecular Predictive Medicine and Sport Science, School of Medicine, Kyorin University, Mitaka, Tokyo 181-8611, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Kizaki
authors.affiliation: Department of Life and Health Sciences, School of Pharma-Sciences, Teikyo University.
authors.forename: Fumiko
authors.initials: F
authors.lastname: Kihara-Negishi
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D009439:Neuraminidase / Q000172:deficiency / Q000378:metabolism*; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000378:metabolism; D000093242:Interleukin-6 Inhibitors
chemical_list: D018932:Chemokine CCL2; D015850:Interleukin-6; D016328:NF-kappa B; D009439:Neuraminidase; D012333:RNA, Messenger; D000093242:Interleukin-6 Inhibitors; C491768:Ccl2 protein, mouse; C110128:Neu1 protein, mouse
keywords: NEU1 sialidase; adipocytes; nuclear factor-kappa B; obesity; pro-inflammatory cytokine