title: Rapid discrimination of blaIMP-1, blaIMP-6, and blaIMP-34using a multiplex PCR.
abstract: Stealth-type carbapenem-resistant Enterobacteriaceae that are resistant to almost all β-lactams except imipenem is emerging in Japan. This resistance is mediated by specific variants of the metallo-β-lactamases (blaIMP-6or blaIMP-34) that differs by one amino acid from the common variant blaIMP-1. We developed an amplification refractory mutation system (ARMS) PCR assay enabling rapid, sequence independent, identification of these variants.
authors.affiliation: Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Japan; Department of Bacteriology, Hiroshima University Graduate School of Biomedical and Health Sciences, Japan.
authors.forename: Shizuo
authors.initials: S
authors.lastname: Kayama
authors.affiliation: Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Japan; Department of Infectious Diseases, Hiroshima University Hospital, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ohge
authors.affiliation: Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Japan; Department of Bacteriology, Hiroshima University Graduate School of Biomedical and Health Sciences, Japan. Electronic address: sugai@hiroshima-u.ac.jp.
authors.forename: Motoyuki
authors.initials: M
authors.lastname: Sugai
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D007564:Japan; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D009154:Mutation; D001618:beta-Lactamases / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D015378:Imipenem; D001618:beta-Lactamases
keywords: ARMS-PCR; Metallo-β-lactamase; Stealth-type resistance phenotype; bla(IMP-34); bla(IMP-6)