title: MiRNA-145 suppresses lung adenocarcinoma cell invasion and migration by targeting N-cadherin.
abstract: OBJECTIVE
  To investigate the roles of miR-145 in lung adenocarcinoma (LAC) and to clarify the regulation of N-cadherin by miR-145.
  
  
  RESULTS
  In 57 paired clinical LAC tissues, diminished miR-145 was significantly correlated with the lymph node metastasis and was negatively correlated with N-cadherin mRNA level expression. Wound healing and transwell assays revealed a reduced capability of tumor metastasis induced by miR-145 in LAC. miR-145 negatively regulated the invasion of cell lines through targeting N-cadherin by directly binding to its 3'-untranslated region. Silencing of N-cadherin inhibited invasion and migration of LAC cell lines similar to miR-145 overexpression.
  
  
  CONCLUSIONS
  MiR-145 could inhibit invasion and migration of lung adenocarcinoma cell lines by directly targeting N-cadherin.
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, People's Republic of China.
authors.forename: Dongping
authors.initials: D
authors.lastname: Mo
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, People's Republic of China.
authors.forename: Daheng
authors.initials: D
authors.lastname: Yang
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, People's Republic of China.
authors.forename: Xuelian
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, People's Republic of China.
authors.forename: Ruihong
authors.initials: R
authors.lastname: Sun
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, People's Republic of China. xu_jian79@163.com.|National Key Clinical Department of Laboratory Medicine, Nanjing, 210029, China. xu_jian79@163.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
mesh_terms: D000072283:A549 Cells; D000230:Adenocarcinoma / Q000453:epidemiology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D000077192:Adenocarcinoma of Lung; D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism*; D015820:Cadherins / Q000235:genetics* / Q000378:metabolism*; D002465:Cell Movement / Q000235:genetics*; D015331:Cohort Studies; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D008175:Lung Neoplasms / Q000453:epidemiology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D035683:MicroRNAs / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics
chemical_list: D015703:Antigens, CD; C050361:CDH2 protein, human; D015820:Cadherins; C541010:MIRN145 microRNA, human; D035683:MicroRNAs
keywords: Invasion; Lung adenocarcinoma; Migration; N-Cadherin; Non-small cell lung cancer; miR-145