title: Application of Inter-Simple Sequence Repeat Markers in the Analysis of Populations of the Chagas Disease VectorTriatoma infestans(Hemiptera, Reduviidae).
abstract: Here we apply inter-simple sequence repeat (ISSR) markers to explore the fine-scale genetic structure and dispersal in populations ofTriatoma infestans. Five selected primers from 30 primers were used to amplify ISSRs by polymerase chain reaction. A total of 90 polymorphic bands were detected across 134 individuals captured from 11 peridomestic sites from the locality of San Martín (Capayán Department, Catamarca Province, Argentina). Significant levels of genetic differentiation suggest limited gene flow among sampling sites. Spatial autocorrelation analysis confirms that dispersal occurs on the scale of ∼469 m, suggesting that insecticide spraying should be extended at least within a radius of ∼500 m around the infested area. Moreover, Bayesian clustering algorithms indicated genetic exchange among different sites analyzed, supporting the hypothesis of an important role of peridomestic structures in the process of reinfestation.
authors.affiliation: Instituto de Investigaciones en Ciencias de la Salud (INICSA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
authors.forename: Alicia R
authors.initials: AR
authors.lastname: Pérez de Rosas
authors.affiliation: Instituto de Investigaciones en Ciencias de la Salud (INICSA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
authors.forename: María F
authors.initials: MF
authors.lastname: Restelli
authors.affiliation: Instituto de Investigaciones en Ciencias de la Salud (INICSA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
authors.forename: Cintia J
authors.initials: CJ
authors.lastname: Fernández
authors.affiliation: Instituto de Investigaciones en Ciencias de la Salud (INICSA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
authors.forename: María J
authors.initials: MJ
authors.lastname: Blariza
authors.affiliation: Instituto de Investigaciones en Ciencias de la Salud (INICSA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
authors.forename: Beatriz A
authors.initials: BA
authors.lastname: García
mesh_terms: D000818:Animals; D001118:Argentina; D001499:Bayes Theorem; D014355:Chagas Disease / Q000517:prevention & control; D053938:DNA Fragmentation; D051456:Gene Flow; D056426:Genetic Loci; D005819:Genetic Markers; D005828:Genetics, Population; D007300:Insect Control; D007303:Insect Vectors / Q000235:genetics / Q000469:parasitology; D007306:Insecticides / Q000494:pharmacology; D018895:Microsatellite Repeats; D020641:Polymorphism, Single Nucleotide; D013048:Specimen Handling; D014224:Triatoma / Q000235:genetics* / Q000469:parasitology
chemical_list: D005819:Genetic Markers; D007306:Insecticides