title: Strategies Involving Mass Spectrometry Combined with Capillary Electrophoresis in Metabolomics.
abstract: This chapter focuses on the important contribution of CE-MS in metabolomics, describing the nature of CE-MS coupling and the technical improvements that have led to the interfaces used in modern instrumentation. Moreover, it will discourse how the variety of electrolyte compositions and additives, which has conferred CE the exceptional selectivity of its multiple separation modes, has been handled to allow interfacing with MS without compromising ionization efficiency and the spectrometer integrity. Finally, the methodologies of CE-MS in current use for metabolomics will be discussed in detail. To verify the scope of CE-MS in clinical metabolomics, a myriad of representative applications has been compiled.
authors.affiliation: Institute of Chemistry, University of São Paulo (USP), 05513-970, São Paulo, SP, Brazil.
authors.forename: Karina Trevisan
authors.initials: KT
authors.lastname: Rodrigues
authors.affiliation: Department of Analytical Chemistry, Institute of Chemistry, University of Campinas (UNICAMP), 13083-970, Campinas, SP, Brazil.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Cieslarová
authors.affiliation: Institute of Chemistry, University of São Paulo (USP), 05513-970, São Paulo, SP, Brazil. mfmtavar@iq.usp.br.
authors.forename: Marina Franco Maggi
authors.initials: MF
authors.lastname: Tavares
authors.affiliation: Department of Analytical Chemistry, Institute of Chemistry, University of Campinas (UNICAMP), 13083-970, Campinas, SP, Brazil. avsimionato@iqm.unicamp.br.|National Institute of Science and Technology in Bioanalytics - INCTBio, Campinas, SP, Brazil. avsimionato@iqm.unicamp.br.
authors.forename: Ana Valéria Colnaghi
authors.initials: AV
authors.lastname: Simionato
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics / Q000379:methods*
keywords: CE-MS; CE-MS interfaces; Capillary electrophoresis; Clinical metabolomics; Metabolomics; Targeted metabolomics; Untargeted metabolomics