title: Altered Expression of Sphingosine-1-Phosphate Metabolizing Enzymes in Oral Cancer Correlate With Clinicopathological Attributes.
abstract: We have determined the gene expression of sphingosine-1-phosphate (S1P) metabolizing enzymes (SphK1, SphK2, SGPL1, SGPP1, SGPP2, PPAP2A, PPAP2B, and PPAP2C) by quantitative real-time polymerase chain reaction in tumor tissues and adjacent normal tissues of 50 oral squamous cell carcinoma (OSCC) patients. Expression of SphK1 and SGPP1 genes was up-regulated significantly in 70% and 75% OSCC tumors respectively. Importantly, expression of SphK2 and PPAP2B was down-regulated in the tumor tissues of 70% OSCC patients. Expression of SphK2 and PPAP2B negatively correlated with tumor-node-metastasis (TNM) staging and tumor volume respectively. Furthermore, LPP1 is an independent predictor of TNM staging and lymph node ratio.
authors.affiliation: a Department of Biochemistry , All India Institute of Medical Sciences (AIIMS) , Saket Nagar, Bhopal , India.
authors.forename: Supriya
authors.initials: S
authors.lastname: Vishwakarma
authors.affiliation: b Jawaharlal Nehru Cancer Hospital & Research Centre (JNCHRC) , Idgah Hills, Bhopal , India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Agarwal
authors.affiliation: a Department of Biochemistry , All India Institute of Medical Sciences (AIIMS) , Saket Nagar, Bhopal , India.
authors.forename: Sudhir K
authors.initials: SK
authors.lastname: Goel
authors.affiliation: c Bansal Hospital and Research Centre , Bhopal , India.
authors.forename: Rajendra K
authors.initials: RK
authors.lastname: Panday
authors.affiliation: b Jawaharlal Nehru Cancer Hospital & Research Centre (JNCHRC) , Idgah Hills, Bhopal , India.
authors.forename: Renu
authors.initials: R
authors.lastname: Singh
authors.affiliation: b Jawaharlal Nehru Cancer Hospital & Research Centre (JNCHRC) , Idgah Hills, Bhopal , India.
authors.forename: Ravi
authors.initials: R
authors.lastname: Sukumaran
authors.affiliation: d Shaheed Bhagat Singh Govt. Degree College, Ashtha, Barkatullah University , Bhopal , India.
authors.forename: Sarita
authors.initials: S
authors.lastname: Khare
authors.affiliation: a Department of Biochemistry , All India Institute of Medical Sciences (AIIMS) , Saket Nagar, Bhopal , India.
authors.forename: Ashok
authors.initials: A
authors.lastname: Kumar
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D008048:Lip Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008246:Lysophospholipids / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D009367:Neoplasm Staging; D010711:Phosphatidate Phosphatase / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000378:metabolism; D014062:Tongue Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D055815:Young Adult; D000099157:Sphingosine Kinase
chemical_list: D008246:Lysophospholipids; D008565:Membrane Proteins; D010711:Phosphatidate Phosphatase; D010744:Phosphoric Monoester Hydrolases; D017853:Phosphotransferases (Alcohol Group Acceptor); D012333:RNA, Messenger; D013110:Sphingosine; D000099157:Sphingosine Kinase; C060506:sphingosine 1-phosphate; C000594250:sphingosine kinase 2, human; C498527:SGPP1 protein, human; C521833:PLPP3 protein, human
keywords: Head and neck cancer; lipid phosphate phosphatase; miR-95-3p; sphingosine kinase 2; sphingosine-1-phosphate