title: Dietary magnesium intake alters age-related changes in rat adipose tissue cellularity.
abstract: Obesity and related metabolic diseases are associated with increased risk of cardiovascular disease. We have previously shown the beneficial effects of dietary magnesium (Mg) supplementation on cardiovascular disease in rats. Therefore, we aimed to examine the effect of an Mg-deficient or supplemented diet on adipose tissue cellularity changes during aging, and on blood pressure (BP), in rats. Male rats received for one (young adult) or 22 months (old), an Mg-deficient (Def) (150 mg/kg), standard (Std) (800 mg/kg) or Mg-supplemented (Sup) (3200 mg/kg) diet. Adipose tissue development and cellularity, BP and leptinemia were evaluated. In rats fed a standard diet, the large increase in adipose tissue weight observed during aging was related to an increase in both size and number of adipocytes. In young adult rats, although adiposity was unchanged, Mg supplementation resulted in a shift of the frequency distribution of adipocytes toward greater sizes, adipose cell weight increasing by 62%. Mg deficiency did not modify adipocyte size, but increased their number (30% more than for the standard or Sup-diet). In old rats, the Def-diet led to relative adipocyte hypotrophy, which was counterbalanced by an increase in the number of adipocyte. Conversely, adipocyte size and number were similar in the Sup-diet and standard diet-fed rats. BP was modified in old rats according to dietary Mg, whereas it remained unchanged young adult rats regardless of the diet received. This study suggests that Mg intake may affect age-related changes in rat adipose tissue lipid storage capacity.
authors.affiliation: EA 4267 Fonctions et dysfonctions épithéliales, faculté de médecine et pharmacie, 25030 Besançon, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Devaux
authors.affiliation: EA 4267 Fonctions et dysfonctions épithéliales, faculté de médecine et pharmacie, 25030 Besançon, France.
authors.forename: Markus
authors.initials: M
authors.lastname: Adrian
authors.affiliation: EA 4267 Fonctions et dysfonctions épithéliales, faculté de médecine et pharmacie, 25030 Besançon, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Laurant
authors.affiliation: EA 4267 Fonctions et dysfonctions épithéliales, faculté de médecine et pharmacie, 25030 Besançon, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Berthelot
authors.affiliation: UMR Physiologie de la nutrition et du comportement alimentaire, AgroParisTech, Inra, Université Paris-Saclay, 75005 Paris.
authors.forename: Annie
authors.initials: A
authors.lastname: Quignard-Boulangé
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D000375:Aging; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D048429:Cell Size / Q000187:drug effects*; D019587:Dietary Supplements; D050356:Lipid Metabolism / Q000187:drug effects; D008274:Magnesium / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D008274:Magnesium
keywords: adipocytes; aging; blood pressure; cellularity; leptin; magnesium