title: MiR-217 promoted the proliferation and invasion of glioblastoma by repressing YWHAG.
abstract: To study the effects of miR-217 on glioblastoma cell proliferation, migration and invasion and its regulation on YWHAG.
  
  
  
  QRT-PCR was used to detect the expression of related mRNAs and miRNA in both glioblastoma tissues and cells. Western blot was used to determine the protein expression of related genes. The transfection was performed using lipo2000. MTT assay, colony formation assay, wound healing assay, Transwell assay as well as flow cytometry were employed to determine the viability, proliferation, migration, invasion and mitosis of UG87 MG cell line. Besides, the dual luciferase reporter gene assay was used to determine the direct targeting relationship between miR-217 and YWHAG. Xenograft models were also constructed and the effect of miR-217 on tumor growth was studied in vivo.
  
  
  
  MiR-217 was up-regulated, whereas YWHAG was down-regulated in glioblastoma tissues and cells. The down-regulation of miR-217 or the up-regulation of YWHAG suppressed the viability, proliferation, migration, invasion and mitosis of U87 MG cells in vitro. In addition, MiR-217 directly targeted 3'UTR of YWHAG and suppressed the expression of YWHAG. Up-regulation of miR-217 could efficiently attenuate the inhibitory effects of YWHAG overexpression on the proliferation and metastasis of U87 MG cells. YWHAG was able to accelerate the phosphorylation of MDM4 and lead to the degradation of P53, which provides a potential mechanism for the tumor-promoting role of miR-217 in glioblastoma cells. By constructing xenograft models, it was also confirmed that miR-217 could promote tumor growth in vivo.
  
  
  
  MiR-217 could promote the viability, proliferation, migration, invasion and mitosis of glioblastoma cells both in vitro and in vivo.
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Hebei University of Engineering, Handan 056029, Hebei, China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Cardiovascular Medicine, Affiliated Hospital of Hebei University of Engineering, Handan 056029, Hebei, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhi
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Hebei University of Engineering, Handan 056029, Hebei, China.
authors.forename: Dongzhou
authors.initials: D
authors.lastname: Ma
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Hebei University of Engineering, Handan 056029, Hebei, China. Electronic address: aiminzhang2000@126.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism*; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D005909:Glioblastoma / Q000378:metabolism* / Q000473:pathology; D064593:Heterografts; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000378:metabolism*; D009368:Neoplasm Transplantation; D012334:RNA, Neoplasm / Q000378:metabolism*
chemical_list: D048948:14-3-3 Proteins; C553315:MIRN217 microRNA, human; D035683:MicroRNAs; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; C000610447:YWHAG protein, human
keywords: Glioblastoma; Overall survival; YWHAG; miR-217