title: Proton-Detected Solid-State NMR Spectroscopy of a Zinc Diffusion Facilitator Protein in Native Nanodiscs.
abstract: The structure, dynamics, and function of membrane proteins are intimately linked to the properties of the membrane environment in which the proteins are embedded. For structural and biophysical characterization, membrane proteins generally need to be extracted from the membrane and reconstituted in a suitable membrane-mimicking environment. Ensuring functional and structural integrity in these environments is often a major concern. The styrene/maleic acid co-polymer has recently been shown to be able to extract lipid/membrane protein patches directly from native membranes to form nanosize discoidal proteolipid particles, also referred to as native nanodiscs. In this work, we show that high-resolution solid-state NMR spectra can be obtained from an integral membrane protein in native nanodiscs, as exemplified by the 2×34 kDa bacterial cation diffusion facilitator CzcD.
authors.affiliation: CEA, CNRS, Univ. Grenoble Alpes, Institut de Biologie Structurale, 71, avenue des martyrs, 38044, Grenoble, France.
authors.forename: Beate
authors.initials: B
authors.lastname: Bersch
authors.affiliation: Membrane Biochemistry and Biophysics, Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands.
authors.forename: Jonas M
authors.initials: JM
authors.lastname: Dörr
authors.affiliation: CEA, CNRS, Univ. Grenoble Alpes, Institut de Biologie Structurale, 71, avenue des martyrs, 38044, Grenoble, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Hessel
authors.affiliation: Membrane Biochemistry and Biophysics, Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands.
authors.forename: J Antoinette
authors.initials: JA
authors.lastname: Killian
authors.affiliation: CEA, CNRS, Univ. Grenoble Alpes, Institut de Biologie Structurale, 71, avenue des martyrs, 38044, Grenoble, France.
authors.forename: Paul
authors.identifier: 0000-0002-9350-7606
authors.initials: P
authors.lastname: Schanda
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D049921:Cupriavidus / Q000737:chemistry*; D004058:Diffusion; D008298:Maleates / Q000737:chemistry*; D026901:Membrane Transport Proteins / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry*; D011510:Proteolipids / Q000737:chemistry; D066244:Proton Magnetic Resonance Spectroscopy / Q000379:methods*; D011522:Protons; D015032:Zinc / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D008298:Maleates; D026901:Membrane Transport Proteins; D011137:Polystyrenes; D011510:Proteolipids; D011522:Protons; C024074:styrene-maleic acid polymer; D015032:Zinc
keywords: NMR spectroscopy; coplymers; membrane proteins; nanodiscs; solid-state NMR