title: Binding site of restriction-modification system controller protein in Mollicutes.
abstract: BACKGROUND
  Bacteria of the class Mollicutes underwent extreme reduction of genomes and gene expression control systems. Only a few regulators are known to date. In this work, we describe a novel group of transcriptional regulators that are distributed within different Mollicutes and control the expression of restriction-modification systems (RM-systems).
  
  
  RESULTS
  We performed cross-species search of putative regulators of RM-systems (C-proteins) and respective binding sites in Mollicutes. We identified a set of novel putative C-protein binding motifs distributed within Mollicutes. We studied the most frequent motif and respective C-protein on the model of Mycoplasma gallisepticum S6. We confirmed our prediction and identified key nucleotides important for C-protein binding. Further we identified novel target promoters of C-protein in M. gallisepticum.
  
  
  CONCLUSIONS
  We found that C-protein of M. gallisepticum binds predicted conserved direct repeats of the (GTGTTAN5)2motif. Apart from its own operon promoter, HsdC can bind to the promoters of the clpB chaperone gene and a tRNA cluster.
authors.affiliation: Federal Research and Clinical Centre of Physical-Chemical Medicine, Malaya Pirogovskaya 1a, Moscow, 119992, Russia. herr.romanoff@gmail.com.
authors.forename: Gleb Y
authors.initials: GY
authors.lastname: Fisunov
authors.affiliation: Federal Research and Clinical Centre of Physical-Chemical Medicine, Malaya Pirogovskaya 1a, Moscow, 119992, Russia.|Department of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Leninskiye Gory, GSP-1, 73, Moscow, 119234, Russia.
authors.forename: Daria V
authors.initials: DV
authors.lastname: Evsyutina
authors.affiliation: Federal Research and Clinical Centre of Physical-Chemical Medicine, Malaya Pirogovskaya 1a, Moscow, 119992, Russia.
authors.forename: Valentin A
authors.initials: VA
authors.lastname: Manuvera
authors.affiliation: Federal Research and Clinical Centre of Physical-Chemical Medicine, Malaya Pirogovskaya 1a, Moscow, 119992, Russia.
authors.forename: Vadim M
authors.initials: VM
authors.lastname: Govorun
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites / Q000502:physiology*; D002874:Chromosome Mapping; D015280:DNA Restriction-Modification Enzymes / Q000235:genetics / Q000378:metabolism*; D049071:Endopeptidase Clp / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics / Q000502:physiology*; D005798:Genes, Bacterial; D045686:Mycoplasma gallisepticum / Q000235:genetics / Q000378:metabolism; D009876:Operon / Q000502:physiology; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D012343:RNA, Transfer / Q000378:metabolism; D008973:Tenericutes / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D015280:DNA Restriction-Modification Enzymes; D014157:Transcription Factors; D012343:RNA, Transfer; D049071:Endopeptidase Clp
keywords: Mollicutes; Mycoplasma; Regulation; Restriction-modification; Transcription factor