title: A Model Prediction for Chenodeoxycholate Aggregate Formation.
abstract: A relationship between the chenodeoxycholate (CDC) monomer concentration and the total concentration of CDC was established using a kinetic dialysis technique. Meanwhile, the sizes of the formed simple CDC micelles were measured by a quasielastic light-scattering (QLS) technique to be nearly constant. The QLS results led to a suggestion for equilibrium models of CDC aggregate formation. According to the established relationship and the suggested models, the best curve-fitting model was selected by a least-squares technique. Furthermore, the model parameters were quantified. Based on the quantified parameters, at a minimum detectable concentration of simple CDC micelles to be ∼0.2 mM, an appropriate model corresponding concentration of CDC monomers was estimated to be ∼3.08 mM. This value is consistent with a minimum monomer CDC concentration of ∼3.13 mM for simple CDC micelle formation estimated according to the present QLS detection and the model prediction. The consistency confirms the model prediction that at a low CDC monomer concentration (<3 mM), the concentration of stable CDC dimers is much higher than that of simple CDC micelles but the contribution of simple CDC micelles to the total CDC concentration cannot be negligible.
authors.affiliation: Biotechnology & Life Science, National Dong-Hwa University, Shou-Feng, Hualien 974, Taiwan.
authors.forename: Yi-Shou
authors.initials: YS
authors.lastname: Lin
authors.affiliation: Biotechnology & Life Science, National Dong-Hwa University, Shou-Feng, Hualien 974, Taiwan.
authors.forename: Kai-Chi
authors.initials: KC
authors.lastname: Su
authors.affiliation: Biotechnology & Life Science, National Dong-Hwa University, Shou-Feng, Hualien 974, Taiwan; College of Chemical Engineering, Huaqiao University, Xiamen 361021, China.
authors.forename: Ranjith K
authors.initials: RK
authors.lastname: Kankala
authors.affiliation: Biotechnology & Life Science, National Dong-Hwa University, Shou-Feng, Hualien 974, Taiwan.
authors.forename: Chia-Hung
authors.initials: CH
authors.lastname: Lee
authors.affiliation: Biotechnology & Life Science, National Dong-Hwa University, Shou-Feng, Hualien 974, Taiwan. Electronic address: cliu@gms.ndhu.edu.tw.
authors.forename: Chen-Lun
authors.initials: CL
authors.lastname: Liu
authors.affiliation: Pharmaceutical Drug Delivery Division, TTY Biopharm Company Limited, Taipei 11469, Taiwan.
authors.forename: Yu-Fang
authors.initials: YF
authors.lastname: Hu
mesh_terms: D002400:Cathartics / Q000737:chemistry / Q000378:metabolism; D002635:Chenodeoxycholic Acid / Q000737:chemistry* / Q000378:metabolism*; D005544:Forecasting; D008956:Models, Chemical; D066329:Protein Aggregates / Q000502:physiology*
chemical_list: D002400:Cathartics; D066329:Protein Aggregates; D002635:Chenodeoxycholic Acid
keywords: curve-fitting; kinetic dialysis technique; quasielastic light-scattering