title: Genetic properties and pathogenicity of a novel reassortant H10N5 influenza virus from wild birds.
abstract: In this study, we analyzed the genome of a H10N5 influenza virus from wild birds. This virus was identified as a novel reassortant virus with internal genes from multiple subtypes and of distinct origins. After sequential passage in mice, mouse-adapted viruses bearing mutations PB2-E627K and HA-G218E were generated. These viruses caused dramatic body weight loss and death, and also replicated in mouse brain, suggesting that the pathogenicity of low pathogenic H10N5 in chickens can be enhanced after passage in mammals. Our data imply that H10N5 viruses might be a potential risk to human health therefore it is important to undertake continued surveillance and biosecurity evaluation of these viruses.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou, 730046, China.
authors.forename: Yane
authors.initials: Y
authors.lastname: Jia
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou, 730046, China.
authors.forename: Jiayun
authors.initials: J
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou, 730046, China.
authors.forename: Zhengxiang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou, 730046, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Du
authors.affiliation: Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou, 730046, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou, 730046, China.
authors.forename: Fengfeng
authors.initials: F
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou, 730046, China.
authors.forename: Maijuan
authors.initials: M
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou, 730046, China.
authors.forename: Shufang
authors.initials: S
authors.lastname: Han
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou, 730046, China. zhuqiyun@caas.cn.
authors.forename: Qiyun
authors.initials: Q
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000821:virology; D001715:Bird Diseases / Q000821:virology*; D001717:Birds / Q000821:virology*; D002642:Chick Embryo; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D006389:Hemagglutinins, Viral / Q000235:genetics*; D005585:Influenza in Birds / Q000821:virology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016865:Reassortant Viruses / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D005819:Genetic Markers; D006389:Hemagglutinins, Viral
keywords: Avian influenza virus; H10N5; Mouse-adapted; Reassortant