title: Preparation and evaluation of microemulsion‑based transdermal delivery of Cistanche tubulosa phenylethanoid glycosides.
abstract: The primary aim of the present study was to develop a novel microemulsion (ME) formulation to deliver phenylethanoid glycoside (PG) for use in skin lighteners and sunscreens. The oil phase was selected on the basis of drug solubility, while the surfactant and cosurfactant were screened and selected on the basis of their solubilizing capacity and the efficiency with which they formed MEs. Pseudoternary phase diagrams were constructed to evaluate ME regions and five formulations of oil‑in‑water MEs were selected as vehicles. In vitro skin permeation experiments were performed to optimize the ME formulation and to evaluate its permeability in comparison to that of saline solution. The physicochemical properties of the optimized ME and the permeating ability of PG delivered by this ME were also investigated. The optimized ME formulation was composed of isopropyl myristate (7%, w/w), Cremorphor EL (21%, w/w), propylene glycol (7%, w/w) and water (65%, w/w). The cumulative amount of PG that permeated through excised mouse skin when carried by ME was ~1.68 times that when PG was carried by saline solution only. The cumulative amount of PG in the microemulsion (4149.650±37.3 µg·cm‑2) was significantly greater than that of PG in the saline solution (2288.63±20.9 µg·cm‑2). Furthermore, the permeability coefficient indicated that optimized microemulsion was a more efficient carrier for transdermal delivery of PG than the control solution (8.87±0.49 cm/hx10‑3 vs. 5.41±0.12 cm/hx10‑3). Taken together, the permeating ability of ME‑carried PG was significantly increased compared with saline solution.
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Natural Medicines, College of Pharmacy, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Natural Medicines, College of Pharmacy, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
authors.forename: Bowei
authors.initials: B
authors.lastname: Ju
authors.affiliation: Department of Natural Medicines, College of Pharmacy, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Department of Natural Medicines, College of Pharmacy, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Natural Medicines, College of Pharmacy, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
authors.forename: Junping
authors.initials: J
authors.lastname: Hu
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D031671:Cistanche / Q000737:chemistry*; D016503:Drug Delivery Systems; D004355:Drug Stability; D004655:Emulsions; D006027:Glycosides / Q000008:administration & dosage* / Q000737:chemistry*; D008297:Male; D051379:Mice; D010539:Permeability; D010626:Phenylethyl Alcohol / Q000737:chemistry*; D028321:Plant Preparations / Q000008:administration & dosage / Q000737:chemistry; D012867:Skin / Q000187:drug effects / Q000378:metabolism; D012995:Solubility
chemical_list: D004655:Emulsions; D006027:Glycosides; D028321:Plant Preparations; D010626:Phenylethyl Alcohol