title: Sanativo Wound Healing Product Does Not Accelerate Reepithelialization in a Mouse Cutaneous Wound Healing Model.
abstract: BACKGROUND
  Sanativo is an over-the-counter Brazilian product derived from Amazon rainforest plant extract that is purported to improve the healing of skin wounds. Two experimental studies have shown accelerated closure of nonsplinted excisional wounds in rat models. However, these models allow for significant contraction of the wound and do not approximate healing in the tight skin of humans.
  
  
  METHODS
  Full-thickness excisional wounds were created on the dorsal skin of mice and were splinted with silicone rings, a model that forces the wound to heal by granulation and reepithelialization. Sanativo or a control solution was applied either daily or every other day to the wounds. Photographs were taken every other day, and the degree of reepithelialization of the wounds was determined.
  
  
  RESULTS
  With both daily and every-other-day applications, Sanativo delayed reepithelialization of the wounds. Average time to complete healing was faster with control solution versus Sanativo in the daily application group (9.4 versus 15.2 days; p < 0.0001) and the every-other-day application group (11 versus 13 days; p = 0.017). The size of visible scar at the last time point of the study was not significantly different between the groups, and no differences were found on histologic examination.
  
  
  CONCLUSIONS
  Sanativo wound healing compound delayed wound reepithelialization in a mouse splinted excisional wound model that approximates human wound healing. The size of visible scar after complete healing was not improved with the application of Sanativo. These results should cast doubt on claims that this product can improve wound healing in humans.
authors.affiliation: Stanford, Calif.|From the Department of Surgery, Division of Plastic and Reconstructive Surgery, and the Department of Bioengineering, Stanford University School of Medicine.
authors.forename: Clement D
authors.initials: CD
authors.lastname: Marshall
authors.affiliation: Stanford, Calif.|From the Department of Surgery, Division of Plastic and Reconstructive Surgery, and the Department of Bioengineering, Stanford University School of Medicine.
authors.forename: Michael S
authors.initials: MS
authors.lastname: Hu
authors.affiliation: Stanford, Calif.|From the Department of Surgery, Division of Plastic and Reconstructive Surgery, and the Department of Bioengineering, Stanford University School of Medicine.
authors.forename: Tripp
authors.initials: T
authors.lastname: Leavitt
authors.affiliation: Stanford, Calif.|From the Department of Surgery, Division of Plastic and Reconstructive Surgery, and the Department of Bioengineering, Stanford University School of Medicine.
authors.forename: Leandra A
authors.initials: LA
authors.lastname: Barnes
authors.affiliation: Stanford, Calif.|From the Department of Surgery, Division of Plastic and Reconstructive Surgery, and the Department of Bioengineering, Stanford University School of Medicine.
authors.forename: Alexander T M
authors.initials: ATM
authors.lastname: Cheung
authors.affiliation: Stanford, Calif.|From the Department of Surgery, Division of Plastic and Reconstructive Surgery, and the Department of Bioengineering, Stanford University School of Medicine.
authors.forename: Samir
authors.initials: S
authors.lastname: Malhotra
authors.affiliation: Stanford, Calif.|From the Department of Surgery, Division of Plastic and Reconstructive Surgery, and the Department of Bioengineering, Stanford University School of Medicine.
authors.forename: H Peter
authors.initials: HP
authors.lastname: Lorenz
authors.affiliation: Stanford, Calif.|From the Department of Surgery, Division of Plastic and Reconstructive Surgery, and the Department of Bioengineering, Stanford University School of Medicine.
authors.forename: Scott L
authors.initials: SL
authors.lastname: Delp
authors.affiliation: Stanford, Calif.|From the Department of Surgery, Division of Plastic and Reconstructive Surgery, and the Department of Bioengineering, Stanford University School of Medicine.
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Quake
authors.affiliation: Stanford, Calif.|From the Department of Surgery, Division of Plastic and Reconstructive Surgery, and the Department of Bioengineering, Stanford University School of Medicine.
authors.forename: Michael T
authors.initials: MT
authors.lastname: Longaker
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D051379:Mice; D008810:Mice, Inbred C57BL; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D062326:Re-Epithelialization / Q000187:drug effects*; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D010936:Plant Extracts; C000620178:sanativo