title: Whole-Genome Haplotyping of Single Sperm of Daphnia pulex (Crustacea, Anomopoda).
abstract: Sequencing the entire genome of single sperm cells can provide valuable information of the distribution of meiotic recombination events in eukaryotic genomes. Here, we provide a description of the experimental work flow for isolating single sperm cells from the microcrustacean Daphnia pulex using fluorescence-activated cell sorting. Moreover, we describe the application of a whole-genome amplification technique (i.e., Multiple Annealing and Looping Based Amplification Cycles method) to single sperm of Daphnia to generate enough DNA for library preparation of next-generation sequencing.
authors.affiliation: Department of Biology, University of Texas at Arlington, 501 S. Nedderman Drive, Life Science Building, Arlington, TX, 76019, USA. exusen@gmail.com.
authors.forename: Sen
authors.initials: S
authors.lastname: Xu
authors.affiliation: Department of Biology, Indiana University, Bloomington, IN, 47405, USA.
authors.forename: Kim
authors.initials: K
authors.lastname: Young
mesh_terms: D000818:Animals; D003621:Daphnia / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D008540:Meiosis / Q000235:genetics; D011995:Recombination, Genetic / Q000235:genetics; D017422:Sequence Analysis, DNA
keywords: Males; Meiosis; Recombination; Single sperm; Whole-genome amplification