title: Improved production of single domain antibodies with two disulfide bonds by co-expression of chaperone proteins in the Escherichia coli periplasm.
abstract: Single domain antibodies are recombinantly expressed variable domains derived from camelid heavy chain antibodies. Natural single domain antibodies can have noncanonical disulfide bonds between their complementarity-determining regions that help position the binding site. In addition, engineering a second disulfide bond serves to tie together β-sheets thereby inhibiting unfolding. Unfortunately, the additional disulfide bond often significantly decreases yield, presumably due to formation of incorrect disulfide bonds during the folding process. Here, we demonstrate that inclusion of the helper plasmid pTUM4, which results in the expression of four chaperones, DsbA, DsbC, FkpA, and SurA, increased yield on average 3.5-fold for the nine multi-disulfide bond single domain antibodies evaluated. No increase in production was observed for single domain antibodies containing only the canonical disulfide bond.
authors.affiliation: US Naval Research Laboratory, Center for Biomolecular Science and Engineering, 4555 Overlook Ave SW, Washington, DC 20375, USA.
authors.forename: Lisa C
authors.initials: LC
authors.lastname: Shriver-Lake
authors.affiliation: US Naval Research Laboratory, Center for Biomolecular Science and Engineering, 4555 Overlook Ave SW, Washington, DC 20375, USA.
authors.forename: Ellen R
authors.initials: ER
authors.lastname: Goldman
authors.affiliation: US Naval Research Laboratory, Center for Biomolecular Science and Engineering, 4555 Overlook Ave SW, Washington, DC 20375, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Zabetakis
authors.affiliation: US Naval Research Laboratory, Center for Biomolecular Science and Engineering, 4555 Overlook Ave SW, Washington, DC 20375, USA. Electronic address: george.anderson@nrl.navy.mil.
authors.forename: George P
authors.initials: GP
authors.lastname: Anderson
mesh_terms: D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods*; D004220:Disulfides / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000096:biosynthesis / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics; D018832:Molecular Chaperones / Q000096:biosynthesis* / Q000235:genetics; D019696:Peptidylprolyl Isomerase / Q000096:biosynthesis / Q000235:genetics; D019897:Periplasm / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D000072757:Protein Conformation, beta-Strand; D011489:Protein Denaturation; D019704:Protein Disulfide-Isomerases / Q000096:biosynthesis / Q000235:genetics; D055550:Protein Stability; D058767:Protein Unfolding; D061905:Single-Domain Antibodies / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D013329:Structure-Activity Relationship; D013696:Temperature
chemical_list: D002352:Carrier Proteins; D004220:Disulfides; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D018832:Molecular Chaperones; D061905:Single-Domain Antibodies; C490900:SurA protein, E coli; D019696:Peptidylprolyl Isomerase; C068254:FkpA protein, E coli; D019704:Protein Disulfide-Isomerases; C526648:dsbA protein, E coli; C526649:dsbC protein, E coli
keywords: Chaperone; Disulfide bond; Helper plasmid; Single domain antibody; Thermal stability