title: Glutamate synthase MoGlt1-mediated glutamate homeostasis is important for autophagy, virulence and conidiation in the rice blast fungus.
abstract: Glutamate homeostasis plays a vital role in central nitrogen metabolism and coordinates several key metabolic functions. However, its function in fungal pathogenesis and development has not been investigated in detail. In this study, we identified and characterized a glutamate synthase gene MoGLT1 in the rice blast fungus Magnaporthe oryzae that was important to glutamate homeostasis. MoGLT1 was constitutively expressed, but showed the highest expression level in appressoria. Deletion of MoGLT1 resulted in a significant reduction in conidiation and virulence. The ΔMoglt1 mutants were defective in appressorial penetration and the differentiation and spread of invasive hyphae in penetrated plant cells. The addition of exogenous glutamic acid partially rescued the defects of the ΔMoglt1 mutants in conidiation and plant infection. Assays for MoAtg8 expression and localization showed that the ΔMoglt1 mutants were defective in autophagy. The ΔMoglt1 mutants were delayed in the mobilization of glycogens and lipid bodies from conidia to developing appressoria. Taken together, our results show that glutamate synthase MoGlt1-mediated glutamate homeostasis is important for pathogenesis and development in the rice blast fungus, possibly via the regulation of autophagy.
authors.affiliation: State Key Laboratory of Agrobiotechnology and Ministry of Agriculture Key Laboratory of Plant Pathology, China Agricultural University, Beijing, 100193, China.|School of Food Science, Henan Institute of Science and Technology, Xinxiang, Henan, 453003, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Agrobiotechnology and Ministry of Agriculture Key Laboratory of Plant Pathology, China Agricultural University, Beijing, 100193, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Agrobiotechnology and Ministry of Agriculture Key Laboratory of Plant Pathology, China Agricultural University, Beijing, 100193, China.
authors.forename: Xiao-Wen
authors.initials: XW
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Agrobiotechnology and Ministry of Agriculture Key Laboratory of Plant Pathology, China Agricultural University, Beijing, 100193, China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Li
authors.affiliation: State Key Laboratory of Agrobiotechnology and Ministry of Agriculture Key Laboratory of Plant Pathology, China Agricultural University, Beijing, 100193, China.
authors.forename: Chang-Fa
authors.initials: CF
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Agrobiotechnology and Ministry of Agriculture Key Laboratory of Plant Pathology, China Agricultural University, Beijing, 100193, China.
authors.forename: Jun-Bo
authors.initials: JB
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Agrobiotechnology and Ministry of Agriculture Key Laboratory of Plant Pathology, China Agricultural University, Beijing, 100193, China.
authors.forename: Song
authors.initials: S
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Agrobiotechnology and Ministry of Agriculture Key Laboratory of Plant Pathology, China Agricultural University, Beijing, 100193, China.
authors.forename: Xiao-Lin
authors.identifier: 0000-0002-5492-516X
authors.initials: XL
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Agrobiotechnology and Ministry of Agriculture Key Laboratory of Plant Pathology, China Agricultural University, Beijing, 100193, China.
authors.forename: Wen-Sheng
authors.initials: WS
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Agrobiotechnology and Ministry of Agriculture Key Laboratory of Plant Pathology, China Agricultural University, Beijing, 100193, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Agrobiotechnology and Ministry of Agriculture Key Laboratory of Plant Pathology, China Agricultural University, Beijing, 100193, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Agrobiotechnology and Ministry of Agriculture Key Laboratory of Plant Pathology, China Agricultural University, Beijing, 100193, China.
authors.forename: You-Liang
authors.initials: YL
authors.lastname: Peng
mesh_terms: D000071182:Autophagosomes / Q000378:metabolism; D001343:Autophagy / Q000235:genetics / Q000502:physiology; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005970:Glutamate Synthase / Q000235:genetics / Q000378:metabolism*; D018698:Glutamic Acid / Q000494:pharmacology; D012275:Oryza / Q000235:genetics / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D014774:Virulence / Q000235:genetics / Q000502:physiology
chemical_list: D005656:Fungal Proteins; D018698:Glutamic Acid; D005970:Glutamate Synthase
keywords: GOGAT; Magnaporthe oryzae; autophagosome; nutrition acquisition; pathogenesis