title: Quantitative iTRAQ Proteomics Revealed Possible Roles for Antioxidant Proteins in Sorghum Aluminum Tolerance.
abstract: Aluminum (Al) toxicity inhibits root growth and limits crop yields on acid soils worldwide. However, quantitative information is scarce on protein expression profiles under Al stress in crops. In this study, we report on the identification of potential Al responsive proteins from root tips of Al sensitive BR007 and Al tolerant SC566 sorghum lines using a strategy employing iTRAQ and 2D-liquid chromatography (LC) coupled to MS/MS (2D-LC-MS/MS). A total of 771 and 329 unique proteins with abundance changes of >1.5 or <0.67-fold were identified in BR007 and SC566, respectively. Protein interaction and pathway analyses indicated that proteins involved in the antioxidant system were more abundant in the tolerant line than in the sensitive one after Al treatment, while opposite trends were observed for proteins involved in lignin biosynthesis. Higher levels of ROS accumulation in root tips of the sensitive line due to decreased activity of antioxidant enzymes could lead to higher lignin production and hyper-accumulation of toxic Al in cell walls. These results indicated that activities of peroxidases and the balance between production and consumption of ROS could be important for Al tolerance and lignin biosynthesis in sorghum.
authors.affiliation: Robert W. Holley Center for Agriculture and Health, United States Department of Agriculture - Agricultural Research Service, Cornell UniversityIthaca, NY, USA; Center of Plateau Ecology, Northwest Institute of Plateau Biology, Chinese Academy of SciencesXining, China.
authors.forename: Dangwei
authors.initials: D
authors.lastname: Zhou
authors.affiliation: Robert W. Holley Center for Agriculture and Health, United States Department of Agriculture - Agricultural Research Service, Cornell University Ithaca, NY, USA.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Robert W. Holley Center for Agriculture and Health, United States Department of Agriculture - Agricultural Research Service, Cornell UniversityIthaca, NY, USA; College of Life Sciences, Fujian Agriculture and Forestry UniversityFuzhou, China.
authors.forename: Jinbiao
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Robert W. Holley Center for Agriculture and Health, United States Department of Agriculture - Agricultural Research Service, Cornell UniversityIthaca, NY, USA; Agricultural Biotechnology Center, Chengdu Institute of Biology, Chinese Academy of SciencesChengdu, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Jiang
authors.affiliation: Robert W. Holley Center for Agriculture and Health, United States Department of Agriculture - Agricultural Research Service, Cornell University Ithaca, NY, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Craft
authors.affiliation: Robert W. Holley Center for Agriculture and Health, United States Department of Agriculture - Agricultural Research Service, Cornell University Ithaca, NY, USA.
authors.forename: Theodore W
authors.initials: TW
authors.lastname: Thannhauser
authors.affiliation: Robert W. Holley Center for Agriculture and Health, United States Department of Agriculture - Agricultural Research Service, Cornell University Ithaca, NY, USA.
authors.forename: Leon V
authors.initials: LV
authors.lastname: Kochian
authors.affiliation: Robert W. Holley Center for Agriculture and Health, United States Department of Agriculture - Agricultural Research Service, Cornell University Ithaca, NY, USA.
authors.forename: Jiping
authors.initials: J
authors.lastname: Liu
keywords: aluminum tolerance; antioxidant system; lignin biosynthesis; proteomics; sorghum