title: Acanthopanaxversus3-Methyladenine Ameliorates Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting the Autophagic Pathway in Rats.
abstract: Objectives. To observe the therapeutic effects of Acanthopanax and 3-methyladenine against severe acute pancreatitis (SAP).Methods. Sodium taurocholate-induced SAP rats were equally randomized into a SAP group, an Acanthopanax group, and a 3-methyladenine group. Serum amylase levels were determined by ELISA; protein and mRNA expression levels of nucleus nuclear factor kappa B (NF-κB) p65, light chain 3II (LC3-II), and Beclin-1 and mRNA expression levels of Class III phosphatidylinositol 3-kinase (PI3K-III) in pancreas tissue were detected by Western blot and quantitative real-time PCR, respectively; mortality and pathological change of the pancreas were observed at 3, 12, and 24 h after operation.Results. There was no significant difference in mortality between SAP group and both treatment groups (P> 0.05). Serum amylase levels, protein, and mRNA expression levels of nucleus NF-κB p65, LC3-II, and Beclin-1 protein, mRNA expression levels of PI3K-III, and pathological score of the pancreas in both treatment groups were significantly lower than those in SAP group at 12 and 24 h after operation (P< 0.05 or 0.01). The number of autophagosomes and autophagolysosomes of pancreatic acinar cells in both treatment groups was smaller than that in SAP group at 12 and 24 h.Conclusions. Acanthopanax and 3-methyladenine had similar therapeutic effects against SAP in rats. The mechanism may be through inhibiting abnormal autophagy activation of pancreatic acinar cells.
authors.affiliation: Department of Gastroenterology, Danyang People's Hospital, Danyang 212300, China.
authors.forename: Xiaohong
authors.identifier: 0000-0002-8755-2531
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong 226001, China.
authors.forename: Guoxiong
authors.initials: G
authors.lastname: Zhou
authors.affiliation: Laboratory Animal Center of Nantong University, Nantong 226001, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology, Danyang People's Hospital, Danyang 212300, China.
authors.forename: Ronglong
authors.initials: R
authors.lastname: Wei
authors.affiliation: Laboratory Animal Center of Nantong University, Nantong 226001, China.
authors.forename: Shunxing
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Department of Gastroenterology, Danyang People's Hospital, Danyang 212300, China.
authors.forename: Yuefen
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Gastroenterology, Danyang People's Hospital, Danyang 212300, China.
authors.forename: Mengjie
authors.initials: M
authors.lastname: Wu
authors.affiliation: Department of Gastroenterology, Danyang People's Hospital, Danyang 212300, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Miao
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D000681:Amylases / Q000097:blood; D000818:Animals; D001343:Autophagy; D028001:Eleutherococcus / Q000737:chemistry*; D008297:Male; D010195:Pancreatitis / Q000139:chemically induced / Q000188:drug therapy*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010936:Plant Extracts / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D013656:Taurocholic Acid
chemical_list: D010936:Plant Extracts; C025946:3-methyladenine; D013656:Taurocholic Acid; D019869:Phosphatidylinositol 3-Kinases; D000681:Amylases; D000225:Adenine