title: Descent of mesonephric duct to the final position of the vas deferens in human embryo and fetus.
abstract: Because the ureter arises from the mesonephric or Wolffian duct (WD), the WD opening should migrate inferiorly along the urogenital sinus or future urethra. However, this process of descent has not been evaluated morphometrically in previous studies and we know little about intermediate morphologies for the descent. In the present work, serial sagittal sections of 15 specimens at gestational age 6-12 weeks and serial horizontal sections of 20 specimens at 6-10 weeks were analyzed. Monitoring of horizontal sections showed that, until 9 weeks, a heart-, lozenge- or oval-shape of the initial urogenital sinus remained in the bladder and urethra. Thus, the future bladder and urethra could not be distinguished by the transverse section or plane. The maximum width of the urogenital sinus or bladder at 6-10 weeks was 0.8 mm, although its supero-inferior length reached 5 mm at 10 weeks. During earlier stages, however, the medial shift of the WD was rather evident. Depending on the extent of upward growth of the bladder smooth muscle, the descent of the vas deferens became evident at 10-12 weeks. Development of the urethral rhabdosphincter likely resulted in the differentiation of urogenital sinus into the urethra and bladder before formation of the bladder neck with 3-layered smooth muscles. Development of the prostate followed these morphological changes, later accelerating the further descent of the WD opening. Because of their close topographical relationships, slight anomalies or accidents of the umbilical cord at 10-12 weeks may have a significant effect on normal anatomy.
authors.affiliation: Department of Anatomy, Histology and Embryology, Yanbian University Medical College, Yanji, China.
authors.forename: Zhe Wu
authors.initials: ZW
authors.lastname: Jin
authors.affiliation: Department of Anatomy, Akita University School of Medicine, Akita, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Abe
authors.affiliation: Department of Urology, Kobe University School of Medicine, Kobe, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Hinata
authors.affiliation: Department of Anatomy, Histology and Embryology, Yanbian University Medical College, Yanji, China.
authors.forename: Xiang Wu
authors.initials: XW
authors.lastname: Li
authors.affiliation: Division of Internal Medicine, Iwamizawa Asuka Hospital, Iwamizawa, Japan.
authors.forename: Gen
authors.initials: G
authors.lastname: Murakami
authors.affiliation: Department of Anatomy and Human Embryology, Institute of Embryology, Faculty of Medicine, Complutense University, Madrid, Spain.
authors.forename: José Francisco
authors.initials: JF
authors.lastname: Rodríguez-Vázquez
keywords: Bladder; Human development; Mesonephric duct; Urogenital sinus; Vas deferens; Wolffian duct