title: Involvement of FvSet1 in Fumonisin B1 Biosynthesis, Vegetative Growth, Fungal Virulence, and Environmental Stress Responses in Fusarium verticillioides.
abstract: Fusarium verticillioides(teleomorph,Gibberella moniliformis) is an important plant pathogen that causes seedling blight, stalk rot, and ear rot in maize (Zea mays). During infection,F. verticillioidesproduce fumonsins B1 (FB1) that pose a serious threat to human and animal health. Recent studies showed that Set1, a methyltransferase of H3K4, was responsible for toxin biosynthesis in filamentous fungi. However, to date, the regulation of FvSet1 on FB1 biosynthesis remains unclear. In the current study, we identified only one Set1 ortholog inF. verticillioides(FvSet1) and found that the deletion ofFvSET1led to various defects in fungal growth and pathogenicity. More interestingly, theFvSET1deletion mutant (ΔFvSet1) showed a significant defect in FB1 biosynthesis and lower expression levels ofFUMgenes. FvSet1 was also found to play an important role in the responses ofF. verticillioidesto multiple environmental stresses via regulating the phosphorylation of FvMgv1 and FvHog1. Taken together, these results indicate that FvSet1 plays essential roles in the regulation of FB1 biosynthesis, fungal growth and virulence, as well as various stress responses inF. verticillioides.
authors.affiliation: Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Ministry of Education, Nanjing 210095, China. guqin@njau.edu.cn.
authors.forename: Qin
authors.initials: Q
authors.lastname: Gu
authors.affiliation: Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Ministry of Education, Nanjing 210095, China. 2014202054@njau.edu.cn.
authors.forename: Hafiz Abdul Samad
authors.initials: HA
authors.lastname: Tahir
authors.affiliation: Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Ministry of Education, Nanjing 210095, China. 2014102015@njau.edu.cn.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Ministry of Education, Nanjing 210095, China. huanghai_201510@163.com.
authors.forename: Hai
authors.initials: H
authors.lastname: Huang
authors.affiliation: Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Ministry of Education, Nanjing 210095, China. 2015102015@njau.edu.cn.
authors.forename: Tiantian
authors.initials: T
authors.lastname: Ji
authors.affiliation: Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Ministry of Education, Nanjing 210095, China. 2016102012@njau.edu.cn.
authors.forename: Xiao
authors.initials: X
authors.lastname: Sun
authors.affiliation: Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Ministry of Education, Nanjing 210095, China. wuliming@njau.edu.cn.
authors.forename: Liming
authors.initials: L
authors.lastname: Wu
authors.affiliation: Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Ministry of Education, Nanjing 210095, China. hjwu@njau.edu.cn.
authors.forename: Huijun
authors.initials: H
authors.lastname: Wu
authors.affiliation: Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Ministry of Education, Nanjing 210095, China. gaoxw@njau.edu.cn.
authors.forename: Xuewen
authors.initials: X
authors.lastname: Gao
mesh_terms: D019295:Computational Biology; D030541:Databases, Genetic; D005516:Food Microbiology; D037341:Fumonisins / Q000378:metabolism*; D005670:Fusarium / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005838:Genotype; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D017384:Sequence Deletion; D013312:Stress, Physiological; D014774:Virulence
chemical_list: D037341:Fumonisins; C056933:fumonisin B1; D011495:Histone-Lysine N-Methyltransferase
keywords: Fusarium verticillioides; FvSet1; fumonisin B1; fungal growth and virulence; stress responses