title: Structural effects of sulfated polysaccharides from Codium fragile on NK cell activation and cytotoxicity.
abstract: The effects of sulfates and proteins of the sulfated polysaccharide-F2(SP-F2) from Codium fragile on the NK cell activation and cytotoxicity were systematically investigated. The SP-F2treatment significantly increased both NK cell proliferation (129%/100μg/mL) and their potent cytotoxic effects against HeLa cells (46%). The SP-F2treatment appeared to enhance NK cell activation through the expression of the activating receptor, NKp30; the secretion of the cytokine, IFN-γ and the release of the lysing proteins, perforin and granzyme-B. However, the treatment of the SP-F2derivatives, deproteinated and desulfated-F2(DP-F2and DS-F2), markedly lowered the mRNA expression levels of IFN-γ, granzyme-B, NKp30 and FasL, suggesting that the proteins and sulfates were essential for the interaction between the SP-F2and NK cells. The antibody neutralization test revealed that CR3 might be a critical receptor involved in SP-F2NK cell activation.
authors.affiliation: Department of Marine Food Science and Technology, Gangneung-Wonju National University, 120 Gangneungdaehangno, Gangneung, Gangwon 210-702, Republic of Korea.
authors.forename: Utoomporn
authors.initials: U
authors.lastname: Surayot
authors.affiliation: Department of Marine Food Science and Technology, Gangneung-Wonju National University, 120 Gangneungdaehangno, Gangneung, Gangwon 210-702, Republic of Korea. Electronic address: umyousg@gwnu.ac.kr.
authors.forename: SangGuan
authors.initials: S
authors.lastname: You
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D000460:Chlorophyta / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects*; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000378:metabolism; D013329:Structure-Activity Relationship; D013451:Sulfonic Acids / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides; D011956:Receptors, Cell Surface; D013451:Sulfonic Acids
keywords: Codium fragile; Cytotoxicity; Natural killer (NK) cells; Polysaccharides