title: Mice with low levels of Shc proteins display reduced glycolytic and increased gluconeogenic activities in liver.
abstract: Shc proteins play a role in energy metabolism through interaction with the insulin receptor. The aim of this study was to determine whether Shc proteins influence liver glycolysis and gluconeogenesis under both fed and fasted states. Decreased glycolytic and increased gluconeogenic and transamination enzyme activities were observed in ShcKO versus WT mice. Levels of key regulatory metabolites, such as fructose-2,6-bisphosphate, matched the activity of metabolic pathways. Protein levels of glycolytic and gluconeogenic enzymes were not different. pAMPK protein levels increased with fasting and were higher in ShcKO versus WT mice. Therefore, Shc proteins play a role in shifting the metabolism from glucose oxidation to gluconeogenesis and lipid catabolism and should be considered as regulators of fuel selection. Fuel selection and utilization could play a critical role in healthy aging. Characterization of metabolic events in ShcKO mice would help to elucidate how metabolism is influenced by these proteins.
authors.affiliation: VM Molecular Biosciences, School of Veterinary Medicine, University of California, Davis 1089 Veterinary Medicine Dr, VM3B, Davis, CA 95616, USA.
authors.forename: Kevork
authors.initials: K
authors.lastname: Hagopian
authors.affiliation: Department of Public Health Sciences, University of California Davis, Davis, CA 95616, USA.
authors.forename: Kyoungmi
authors.initials: K
authors.lastname: Kim
authors.affiliation: VM Molecular Biosciences, School of Veterinary Medicine, University of California, Davis 1089 Veterinary Medicine Dr, VM3B, Davis, CA 95616, USA.
authors.forename: José Alberto
authors.initials: JA
authors.lastname: López-Dominguez
authors.affiliation: VM Molecular Biosciences, School of Veterinary Medicine, University of California, Davis 1089 Veterinary Medicine Dr, VM3B, Davis, CA 95616, USA.
authors.forename: Alexey A
authors.initials: AA
authors.lastname: Tomilov
authors.affiliation: VM Molecular Biosciences, School of Veterinary Medicine, University of California, Davis 1089 Veterinary Medicine Dr, VM3B, Davis, CA 95616, USA.
authors.forename: Gino A
authors.initials: GA
authors.lastname: Cortopassi
authors.affiliation: VM Molecular Biosciences, School of Veterinary Medicine, University of California, Davis 1089 Veterinary Medicine Dr, VM3B, Davis, CA 95616, USA.
authors.forename: Jon J
authors.initials: JJ
authors.lastname: Ramsey
keywords: Fasting; Fuel selection; Gluconeogenesis; Glycolysis; Metabolism; Shc proteins