title: Discovery of gastric cancer specific biomarkers by the application of serum proteomics.
abstract: Current diagnostic markers for gastric cancer are not sufficiently specific or sensitive for use in clinical practice. The aims of this study are to compare the proteomes of serum samples from patients with gastric cancers and normal controls, and to develop useful tumor markers of gastric cancer by quantitative proteomic analysis. We identified a total of 388 proteins with a ≤1% FDR and with at least two unique peptides from the sera of each group. Among them, 215, 251, and 260 proteins were identified in serum samples of patients in an advanced cancer group, early cancer group, and normal control group, respectively. We selected differentially expressed proteins in cancer patients compared with those of normal controls via semiquantitative analyses comparing the spectral counts of identified proteins. These differentially expressed proteins were successfully verified using an MS-based quantitative assay, multiple reactions monitoring analysis. Four proteins (vitronectin, clusterin isoform 1, thrombospondin 1, and tyrosine-protein kinase SRMS) were shown to have significant changes between the cancer groups and the normal control group. These four serum proteins were able to discriminate gastric cancer patients from normal controls with sufficient specificity and selectivity.
authors.affiliation: Department of Surgery, Konkuk University School of Medicine, Seoul, Republic of Korea.
authors.forename: Moon-Won
authors.initials: MW
authors.lastname: Yoo
authors.affiliation: Department of Applied Chemistry, College of Applied Sciences, Kyung Hee University, Yong-in City, Republic of Korea.
authors.forename: Jisook
authors.initials: J
authors.lastname: Park
authors.affiliation: Department of Pathology, Konkuk University School of Medicine, Seoul, Republic of Korea.
authors.forename: Hye-Seung
authors.initials: HS
authors.lastname: Han
authors.affiliation: Department of Laboratory Medicine, Konkuk University School of Medicine, Seoul, Republic of Korea.
authors.forename: Yeo-Min
authors.initials: YM
authors.lastname: Yun
authors.affiliation: Department of Applied Chemistry, College of Applied Sciences, Kyung Hee University, Yong-in City, Republic of Korea.
authors.forename: Jeong Won
authors.initials: JW
authors.lastname: Kang
authors.affiliation: Department of Applied Chemistry, College of Applied Sciences, Kyung Hee University, Yong-in City, Republic of Korea.
authors.forename: Do-Young
authors.initials: DY
authors.lastname: Choi
authors.affiliation: Department of Applied Chemistry, College of Applied Sciences, Kyung Hee University, Yong-in City, Republic of Korea.
authors.forename: Joon Won
authors.initials: JW
authors.lastname: Lee
authors.affiliation: Department of Applied Chemistry, College of Applied Sciences, Kyung Hee University, Yong-in City, Republic of Korea.
authors.forename: Jae Hun
authors.initials: JH
authors.lastname: Jung
authors.affiliation: Department of Surgery, Konkuk University School of Medicine, Seoul, Republic of Korea.
authors.forename: Kyung-Yung
authors.initials: KY
authors.lastname: Lee
authors.affiliation: Department of Applied Chemistry, College of Applied Sciences, Kyung Hee University, Yong-in City, Republic of Korea.
authors.forename: Kwang Pyo
authors.initials: KP
authors.lastname: Kim
mesh_terms: D000328:Adult; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D063990:Gene Ontology; D006801:Humans; D008297:Male; D008875:Middle Aged; D040901:Proteomics / Q000379:methods*; D012372:ROC Curve; D015203:Reproducibility of Results; D013223:Statistics as Topic; D013274:Stomach Neoplasms / Q000097:blood* / Q000175:diagnosis*
chemical_list: D014408:Biomarkers, Tumor
keywords: Biomarker; Gastric cancer; Mass spectrometry; Multiple reaction monitoring; Serum proteomics