title: 7,8-DHF Treatment Induces Cyr61 Expression to Suppress Hypoxia Induced ER Stress in HK-2 Cells.
abstract: Acute kidney injury (AKI) is a common syndrome which is strongly linked to high morbidity and mortality. Hypoxia is the leading cause of AKI and the proximal renal tubular cells are the most damaged part in the kidney during this period. It has been observed that 7,8-dihydroxyflavone (7,8-DHF) plays a protective role by acting on antiapoptosis and antioxidative stress. In this study we explored functions of 7,8-DHF in protecting human proximal tubular cell line HK-2 from hypoxia insults. We observed that treatment of 7,8-DHF could improve the viability of ischemic cell. Mechanistically, we found that 7,8-DHF could elevate the expression of cysteine-rich protein 61 (Cyr61), a protective immediate early gene in AKI. In addition, treatment of 7,8-DHF decreased CCAAT/enhancer-binding protein homologous protein (CHOP) expression, which is a marker protein during endoplasmic reticulum (ER) stress activation. Intriguingly, overexpression of Cyr61 significantly reduced CHOP expression. Taken together, our results provide novel insights into the possible protective role of 7,8-DHF by activating Cyr61 signaling and suppressing ER stress in hypoxic HK-2 cells which have potential clinical implications for the treatment of AKI.
authors.affiliation: Department of Nephrology, Affiliated Hospital of Qingdao University, Qingdao 266003, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Ma
authors.affiliation: Key Laboratory, Department of Otolaryngology-Head and Neck Surgery, Affiliated Hospital of Qingdao University, Qingdao 266003, China.
authors.forename: Jisheng
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Nephrology, Affiliated Hospital of Qingdao University, Qingdao 266003, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Nephrology, Affiliated Hospital of Qingdao University, Qingdao 266003, China.
authors.forename: Shaoheng
authors.initials: S
authors.lastname: Yue
authors.affiliation: Department of Nephrology, Affiliated Hospital of Qingdao University, Qingdao 266003, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Department of Nephrology, Affiliated Hospital of Qingdao University, Qingdao 266003, China.
authors.forename: Yan
authors.identifier: 0000-0003-2049-3976
authors.initials: Y
authors.lastname: Xu
mesh_terms: D015687:Cell Hypoxia / Q000187:drug effects* / Q000502:physiology*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects* / Q000502:physiology*; D044950:Flavanones / Q000008:administration & dosage*; D006801:Humans; D007684:Kidney Tubules / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
chemical_list: C478869:7,8-dihydroxyflavanone; D044950:Flavanones