title: Microfracture combined with functional pig peritoneum-derived acellular matrix for cartilage repair in rabbit models.
abstract: Due to avascular and hypocellular nature of cartilage, repair of articular cartilage defects within synovial joints still poses a significant clinical challenge. To promote neocartilage properties, we established a functional scaffold named APM-E7 by conjugating a bone marrow-derived mesenchymal stem cell (BM-MSC) affinity peptide (E7) onto the acellular peritoneum matrix (APM). During in vitro culture, the APM-E7 scaffold can support better proliferation as well as better differentiation into chondrocytes of BM-MSCs. After implanting into cartilage defects in rabbits for 24weeks, compared with microfracture and APM groups, the APM-E7 scaffolds exhibited superior quality of neocartilage without transplant rejection, according to general observations, histological assessment, synovial fluid analysis, magnetic resonance imaging (MRI) and nanomechanical properties. This APM-E7 scaffold provided a scaffold for cell attachment, which was crucial for cartilage regeneration. Overall, the APM-E7 is a promising biomaterial with low immunogenicity for one-step cartilage repair by promoting autologous connective tissue progenitor (CTP) attachment.
  
  
  
  We report the one-step transplantation of functional acellular peritoneum matrix (APM-E7) with specific mesenchymal stem cell recruitment to repair rabbit cartilage injury. The experimental results illustrated that the APM-E7 scaffold was successfully fabricated, which could specifically recruit MSCs and fill the cartilage defects in the femoral trochlear of rabbits at 24weeks post-surgery. The repaired tissue was hyaline cartilage, which exhibited ideal mechanical stability. The APM-E7 biomaterial could provide scaffold for MSCs and improve cell homing, which are two key factors required for cartilage tissue engineering, thereby providing new insights into cartilage tissue engineering.
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, People's Republic of China.
authors.forename: Qingyang
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, People's Republic of China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Hu
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, People's Republic of China.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Huang
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, People's Republic of China.
authors.forename: Zhenlong
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Medical and Healthy Analysis Centre, Peking University, 38 Xueyuan Road, Haidian District, Beijing 100191, People's Republic of China.
authors.forename: Lan
authors.initials: L
authors.lastname: Yuan
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, People's Republic of China.
authors.forename: Zhenxing
authors.initials: Z
authors.lastname: Shao
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, People's Republic of China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, People's Republic of China.
authors.forename: Jiying
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Fu
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, People's Republic of China.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Duan
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, People's Republic of China. Electronic address: aoyingfang@163.com.
authors.forename: Yingfang
authors.initials: Y
authors.lastname: Ao
mesh_terms: D000818:Animals; D002474:Cell-Free System / Q000737:chemistry; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005109:Extracellular Matrix / Q000737:chemistry*; D050724:Fractures, Cartilage / Q000473:pathology / Q000628:therapy*; D015775:Fractures, Stress / Q000473:pathology / Q000628:therapy*; D045164:Mesenchymal Stem Cell Transplantation / Q000295:instrumentation* / Q000379:methods; D010537:Peritoneum / Q000737:chemistry* / Q000166:cytology*; D011817:Rabbits; D013552:Swine; D054457:Tissue Scaffolds; D016896:Treatment Outcome
keywords: Acellular peritoneum matrix; Cartilage tissue engineering; Functional modification; MSC affinity peptide