title: The Celiac Patient Antibody Response to Conventional and Gluten-Removed Beer.
abstract: Enzymatic digestion, or hydrolysis, has been proposed for treating gluten-containing foods and beverages to make them safe for persons with celiac disease (CD). There are no validated testing methods that allow the quantitation of all the hydrolyzed or fermented gluten peptides in foods and beverages that might be harmful to CD patients, making it difficult to assess the safety of hydrolyzed products. This study examines an ELISA-based method to determine whether serum antibody binding of residual peptides in a fermented barley-based product is greater among active-CD patients than a normal control group, using commercial beers as a test case. Sera from 31 active-CD patients and 29 nonceliac control subjects were used to assess the binding of proteins from barley, rice, traditional beer, gluten-free beer, and enzymatically treated (gluten-removed) traditional beer. In the ELISA, none of the subjects' sera bound to proteins in the gluten-free beer. Eleven active-CD patient serum samples demonstrated immunoglobulin A (IgA) or immunoglobulin G (IgG) binding to a barley extract, compared to only one nonceliac control subject. Of the seven active-CD patients who had an IgA binding response to barley, four also responded to traditional beer, and two of these responded to the gluten-removed beer. None of the nonceliac control subjects' sera bound to all three beer samples. Binding of protein fragments in hydrolyzed or fermented foods and beverages by serum from active-CD patients, but not nonceliac control subjects, may indicate the presence of residual peptides that are celiac-specific.
authors.affiliation: Gluten Intolerance Group of North America, Auburn, WA.
authors.forename: Laura K
authors.initials: LK
authors.lastname: Allred
authors.affiliation: The University of Chicago, Biological Sciences Division, Chicago, IL.
authors.forename: Katherine
authors.initials: K
authors.lastname: Lesko
authors.affiliation: The University of Chicago Celiac Disease Center, Chicago, IL.
authors.forename: Diane
authors.initials: D
authors.lastname: McKiernan
authors.affiliation: Gluten Intolerance Group of North America, Auburn, WA.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Kupper
authors.affiliation: University of Chicago, Department of Pediatrics, Section of Pediatric Gastroenterology, Hepatology, and Nutrition, Chicago, IL.
authors.forename: Stefano
authors.initials: S
authors.lastname: Guandalini
mesh_terms: D000328:Adult; D000917:Antibody Formation; D001515:Beer; D002446:Celiac Disease / Q000276:immunology*; D005433:Flour / Q000032:analysis; D005903:Gliadin / Q000276:immunology*; D001467:Hordeum / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood* / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D012275:Oryza / Q000276:immunology; D045868:Sorghum / Q000276:immunology
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G; D005903:Gliadin