title: Anticancer Effects of the Marine SpongeLipastrotethyasp. Extract on Wild-Type and p53 Knockout HCT116 Cells.
abstract: Interest in marine bioresources is increasing in the drug development sector. In particular, marine sponges produce a wide range of unique metabolites that enable them to survive in challenging environments, which makes them attractive sources of candidate pharmaceuticals. In previous study, we investigated over 40 marine specimens collected in Micronesia and provided by the Korean Institute of Ocean Science and Technology, for their antiproliferative effects on various cancer cell lines, andLipastrotethyasp. extract (LSSE) was found to have a marked antiproliferative effect. In the present study, we investigated the mechanism responsible for its anticancer effect on wild-type p53 (WT) or p53 knockout (KO) HCT116 cells. LSSE inhibited cell viability and induced apoptotic cell death more so in HCT116 p53 KO cells than the WT. HCT116 WT cells treated with LSSE underwent apoptosis associated with the induction of p53 and its target genes. On the other hand, in HCT116 p53 KO cells, LSSE reduced mTOR and Bcl-2 and increased Beclin-1 and LC3-II protein levels, suggesting autophagy induction. These results indicate that the mechanisms responsible for the anticancer effect of LSSE depend on p53 status.
authors.affiliation: College of Pharmacy, Duksung Women's University, Seoul 01369, Republic of Korea; Innovative Drug Center, Duksung Women's University, Seoul 01369, Republic of Korea.
authors.forename: Kiheon
authors.initials: K
authors.lastname: Choi
authors.affiliation: Innovative Drug Center, Duksung Women's University, Seoul 01369, Republic of Korea.
authors.forename: Hyun Kyung
authors.initials: HK
authors.lastname: Lim
authors.affiliation: Innovative Drug Center, Duksung Women's University, Seoul 01369, Republic of Korea.
authors.forename: Sung Ryong
authors.initials: SR
authors.lastname: Oh
authors.affiliation: College of Pharmacy, Duksung Women's University, Seoul 01369, Republic of Korea; Innovative Drug Center, Duksung Women's University, Seoul 01369, Republic of Korea.
authors.forename: Woo-Hyun
authors.initials: WH
authors.lastname: Chung
authors.affiliation: College of Pharmacy, Duksung Women's University, Seoul 01369, Republic of Korea; Innovative Drug Center, Duksung Women's University, Seoul 01369, Republic of Korea.
authors.forename: Joohee
authors.identifier: 0000-0001-9124-9052
authors.initials: J
authors.lastname: Jung