title: Characterization and Expression Analysis of Common BeanHistone Deacetylase 6during Development and Cold Stress Response.
abstract: Histone deacetylases (HDACs) are important regulators of gene transcription thus controlling multiple cellular processes. Despite its essential role in plants,HDA6is yet to be validated in common bean. In this study, we show thatHDA6is involved in plant development and stress response. Differential expression ofHDA6was determined in various tissues and the expression was seen to be upregulated with plant age (seedling < flowering < maturity). Higher expression was observed in flowers and pods than in stem, leaf, and root. Upregulation ofHDA6gene during cold stress implies its prominent role in abiotic stress. Furthermore, theHDA6gene was isolated from three common bean genotypes and sequence analyses revealed homology with functionally characterized homologs in model species. The 53 kDa translated product was detected using anHDA6specific antibody and recombinant protein overexpressed inEscherichia colishowed HDAC activityin vitro. To our knowledge, this is the first report in the agriculturally important crop common bean describing the functional characterization and biological role ofHDA6.
authors.affiliation: Molecular Genetics and EpiGenomics Laboratory, Department of Agriculture and Natural Resources, Delaware State University, Dover, DE 19901, USA.
authors.forename: Rita Kusi-Appiah
authors.identifier: 0000-0002-8583-9432
authors.initials: RK
authors.lastname: Hayford
authors.affiliation: Plant Biotechnology Laboratory, Department of Agriculture and Natural Resources, Delaware State University, Dover, DE 19901, USA.
authors.forename: Ayalew
authors.initials: A
authors.lastname: Ligaba-Osena
authors.affiliation: Molecular Genetics and EpiGenomics Laboratory, Department of Agriculture and Natural Resources, Delaware State University, Dover, DE 19901, USA.
authors.forename: Mayavan
authors.initials: M
authors.lastname: Subramani
authors.affiliation: Molecular Genetics and EpiGenomics Laboratory, Department of Agriculture and Natural Resources, Delaware State University, Dover, DE 19901, USA.
authors.forename: Adrianne
authors.initials: A
authors.lastname: Brown
authors.affiliation: Molecular Genetics and EpiGenomics Laboratory, Department of Agriculture and Natural Resources, Delaware State University, Dover, DE 19901, USA; Center for Integrated Biological and Environmental Research (CIBER), Delaware State University, Dover, DE 1990, USA.
authors.forename: Kalpalatha
authors.initials: K
authors.lastname: Melmaiee
authors.affiliation: Science and Mathematics Division, Mayville State University, Mayville, ND 58255, USA.
authors.forename: Khwaja
authors.initials: K
authors.lastname: Hossain
authors.affiliation: Molecular Genetics and EpiGenomics Laboratory, Department of Agriculture and Natural Resources, Delaware State University, Dover, DE 19901, USA; Center for Integrated Biological and Environmental Research (CIBER), Delaware State University, Dover, DE 1990, USA.
authors.forename: Venu Kal
authors.identifier: 0000-0002-3353-3968
authors.initials: VK
authors.lastname: Kalavacharla