title: Selective Luminescent Labeling of DNA and RNA Quadruplexes by π-Extended Ruthenium Light-Up Probes.
abstract: A series of RuIIcomplexes exhibiting π-extended, acridine-based ancillary chelating heterocycles display high affinity and selectivity for DNA and RNA quadruplexes. The most promising candidates (3, 4) possess remarkable light-up luminophore properties (up to 330-fold luminescence enhancement upon interaction with quadruplexes), enabling them to discriminate quadruplexes from genomic DNA owing to a photochemical mechanism involving DNA protection against non-radiative decay (DAND), thus deviating from the other complexes of this series of ligands that exhibit an excited-state intramolecular proton transfer (ESIPT) that quenches their luminescence. The in vitro and preliminary in cellulo results shown here confirm the interest of this new family of fluorophores as invaluable molecular tools to detect G-quadruplexes in cells.
authors.affiliation: Laboratoire de Chimie Organique et Photochimie, Université Libre de Bruxelles, 1050, Bruxelles, Belgium.|Departement de Pharmacochimie Moléculaire, Université Grenoble Alpes, CNRS UMR5063, 38041, Grenoble, France.
authors.forename: Dounia
authors.initials: D
authors.lastname: Saadallah
authors.affiliation: Institut de Chimie Moléculaire, ICMUB CNRS UMR6302, UBFC, 21078, Dijon, France.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Bellakhal
authors.affiliation: Institut de Chimie Moléculaire, ICMUB CNRS UMR6302, UBFC, 21078, Dijon, France.
authors.forename: Souheila
authors.initials: S
authors.lastname: Amor
authors.affiliation: Departement de Pharmacochimie Moléculaire, Université Grenoble Alpes, CNRS UMR5063, 38041, Grenoble, France.|Laboratoire de Chimie et Biologie des Métaux, Université Grenoble Alpes, CNRS UMR5249, CEA, 38054, Grenoble, France.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Lefebvre
authors.affiliation: Laboratoire de Chimie et Biologie des Métaux, Université Grenoble Alpes, CNRS UMR5249, CEA, 38054, Grenoble, France.
authors.forename: Murielle
authors.initials: M
authors.lastname: Chavarot-Kerlidou
authors.affiliation: Departement de Pharmacochimie Moléculaire, Université Grenoble Alpes, CNRS UMR5063, 38041, Grenoble, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Baussanne
authors.affiliation: Laboratoire de Chimie Organique et Photochimie, Université Libre de Bruxelles, 1050, Bruxelles, Belgium.
authors.forename: Cécile
authors.initials: C
authors.lastname: Moucheron
authors.affiliation: Departement de Pharmacochimie Moléculaire, Université Grenoble Alpes, CNRS UMR5063, 38041, Grenoble, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Demeunynck
authors.affiliation: Institut de Chimie Moléculaire, ICMUB CNRS UMR6302, UBFC, 21078, Dijon, France.
authors.forename: David
authors.identifier: http://orcid.org/0000-0002-3056-9295
authors.initials: D
authors.lastname: Monchaud
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D054856:G-Quadruplexes; D008024:Ligands; D009942:Organometallic Compounds / Q000737:chemistry*; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D012428:Ruthenium / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D008024:Ligands; D009942:Organometallic Compounds; D012313:RNA; D012428:Ruthenium; D004247:DNA
keywords: DNA/RNA-quadruplexes; ESIPT; light-up probes; luminescence; ruthenium complexes