title: Novel feed including bioactive compounds from winery wastes improved broilers' redox status in blood and tissues of vital organs.
abstract: Currently, there is a great interest in the production of animal feed with antioxidant activity. The aim of this study was to examine the potential antioxidant effects of a feed supplemented with grape pomace (GP), a winery by-product with high environmental load, in chickens. Broilers of 15 days post birth were separated into two groups fed either with standard diet or with diet supplemented with GP for 35 days. Blood and tissues collections were performed after feeding for 15 and 35 days with the experimental diet (i.e. at 30 and 50 days post birth). Free radical toxicity markers, namely thiobarbituric acid reactive substances, protein carbonyls, total antioxidant capacity, reduced glutathione, catalase activity and rate of H2O2decomposition were determined in blood and tissues of vital organs. The results indicated that feed supplemented with GP decreased oxidative stress-induced toxic effects and improved chickens' redox status, and so it may also improve their wellness and productivity. On the other hand, this exploitation of GP may solve problems of environmental pollution in areas with wineries.
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis, Mezourlo, 41334, Larissa, Greece.
authors.forename: Sotiria
authors.initials: S
authors.lastname: Makri
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis, Mezourlo, 41334, Larissa, Greece.
authors.forename: Ioannis
authors.initials: I
authors.lastname: Kafantaris
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis, Mezourlo, 41334, Larissa, Greece.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Stagos
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis, Mezourlo, 41334, Larissa, Greece.
authors.forename: Theodora
authors.initials: T
authors.lastname: Chamokeridou
authors.affiliation: Department of Biosystem Engineering, Technical Education Institute of Thessaly, 41110, Larissa, Greece.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Petrotos
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis, Mezourlo, 41334, Larissa, Greece; Department of Biosystem Engineering, Technical Education Institute of Thessaly, 41110, Larissa, Greece.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Gerasopoulos
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis, Mezourlo, 41334, Larissa, Greece.
authors.forename: Anastasios
authors.initials: A
authors.lastname: Mpesios
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis, Mezourlo, 41334, Larissa, Greece.
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Goutzourelas
authors.affiliation: Department of Biosystem Engineering, Technical Education Institute of Thessaly, 41110, Larissa, Greece.
authors.forename: Stylianos
authors.initials: S
authors.lastname: Kokkas
authors.affiliation: Department of Biosystem Engineering, Technical Education Institute of Thessaly, 41110, Larissa, Greece.
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Goulas
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis, Mezourlo, 41334, Larissa, Greece.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Komiotis
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis, Mezourlo, 41334, Larissa, Greece. Electronic address: dkouret@uth.gr.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Kouretas
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D001769:Blood / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002645:Chickens / Q000378:metabolism*; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D014866:Waste Products; D014920:Wine
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D016166:Free Radical Scavengers; D014866:Waste Products; D002374:Catalase; D005978:Glutathione
keywords: Antioxidants; Broilers; Grape pomace; Polyphenols; Toxicity markers; Winery waste