title: Data supporting the effects of lysozyme on mRNA and protein expression in a colonic epithelial scratch wound model.
abstract: Colonic epithelial health is implicated in a host of gastrointestinal (GI) diseases and disorders. Lysozyme is suspected to play a role in the ability of the epithelium to recover from injury (Abey et al., in press; Gallo, 2012; Rubio, 2014) [1], [2], [3]. Disrupted repair mechanisms may lead to delayed or ineffective recovery and disruptions to epithelial biology resulting in GI symptoms and altered barrier function (Peterson and Artis, 2014) [4]. The effect of lysozyme on the transcriptomic and proteomic profile of healthy colonic epithelial cells was investigated. Epithelial cells in culture were scratch wounded and treated with lysozyme. mRNA and protein profiles were simultaneously quantified in the same sample using a digital counting technology. Gene and protein expressions altered by the presence or absence of lysozyme are described in this article. Extensive statistical and bioinformatic analysis, and interpretation of the results can be found in "Lysozyme association with circulating RNA, extracellular vesicles, and chronic stress" (Abey et al., in press) [1].
authors.affiliation: Digestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA.
authors.forename: Sarah K
authors.initials: SK
authors.lastname: Abey
authors.affiliation: Image Sciences Institute, Division of Imaging, University Medical Centre Utrecht, Netherlands.
authors.forename: Yuana
authors.initials: Y
authors.lastname: Yuana
authors.affiliation: Digestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA.
authors.forename: Paule V
authors.initials: PV
authors.lastname: Joseph
authors.affiliation: Digestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA.
authors.forename: Natnael D
authors.initials: ND
authors.lastname: Kenea
authors.affiliation: Digestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA.
authors.forename: Nicolaas H
authors.initials: NH
authors.lastname: Fourie
authors.affiliation: Digestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA.
authors.forename: LeeAnne B
authors.initials: LB
authors.lastname: Sherwin
authors.affiliation: NanoString Technologies, Seattle, WA, USA.
authors.forename: Gregory E
authors.initials: GE
authors.lastname: Gonye
authors.affiliation: The Pennsylvania State University, College of Medicine, Hershey, PA, USA.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Smyser
authors.affiliation: Electron Microscopy Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA.
authors.forename: Erin S
authors.initials: ES
authors.lastname: Stempinski
authors.affiliation: Digestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA.
authors.forename: Christina M
authors.initials: CM
authors.lastname: Boulineaux
authors.affiliation: Digestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA.
authors.forename: Kristen R
authors.initials: KR
authors.lastname: Weaver
authors.affiliation: Electron Microscopy Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA.
authors.forename: Christopher K E
authors.initials: CK
authors.lastname: Bleck
authors.affiliation: Digestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA.
authors.forename: Wendy A
authors.initials: WA
authors.lastname: Henderson