title: Enrofloxacin in therapeutic doses alters cytokine production by porcine PBMCs induced by lipopolysaccharide.
abstract: The effect of enrofloxacin on cytokine secretion by porcine peripheral blood mononuclear cells (PBMCs) was studied. Twenty 8-20-week-old pigs were randomly divided into two groups: control (C, n = 10) and experimental (E, n = 10) were used. Pigs from group E received enrofloxacin at therapeutic dose for 5 consecutive days. Blood samples were collected at 0 (before antibiotic administration), 2, 4 (during antibiotic therapy) 6, 9, 14 21, 35, 49, and 63 d of study (after treatment). PBMCs of pigs from both groups were incubated with or without lipopolysaccharide (LPS). Ex vivo production on interleukin (IL)-4, IL-6, IL-10, INF-γ, and TNF-α were analyzed using ELISA assay. Intramuscular administration of enrofloxacin to healthy pigs for 5 consecutive days induced a transitory reduction of the ex vivo response of PBMCs to LPS in terms of IL-6 and TNF-α secretion. The level of IL-6 returned to day 0 level shortly after end of treatment, while the TNF-α production remained reduced 10 d after the end of treatment. Our results indicate that enrofloxacin given in vivo in therapeutic doses has an immunomodulatory effect through its capacity to inhibit ex vivo secretion of IL-6 and TNF-α by porcine PBMC after LPS stimulation.
authors.affiliation: a Department of Swine Diseases , National Veterinary Research Institute , Pulawy , Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Pomorska-Mól
authors.affiliation: a Department of Swine Diseases , National Veterinary Research Institute , Pulawy , Poland.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Czyżewska-Dors
authors.affiliation: a Department of Swine Diseases , National Veterinary Research Institute , Pulawy , Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Kwit
authors.affiliation: a Department of Swine Diseases , National Veterinary Research Institute , Pulawy , Poland.
authors.forename: Zygmunt
authors.initials: Z
authors.lastname: Pejsak
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D000077422:Enrofloxacin; D005260:Female; D024841:Fluoroquinolones / Q000494:pharmacology*; D056747:Immunomodulation / Q000187:drug effects*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology*; D008297:Male; D013552:Swine
chemical_list: D000890:Anti-Infective Agents; D016207:Cytokines; D024841:Fluoroquinolones; D008070:Lipopolysaccharides; C440499:lipopolysaccharide, Escherichia coli O111 B4; D000077422:Enrofloxacin
keywords: Fluoroquinolone; LPS; cytokine; immunomodulation; swine