title: Long noncoding RNA FTX is upregulated in gliomas and promotes proliferation and invasion of glioma cells by negatively regulating miR-342-3p.
abstract: Gliomas remain a major public health challenge, posing a high risk for brain tumor-related morbidity and mortality. However, the mechanisms that drive the development of gliomas remain largely unknown. Emerging evidence has shown that long noncoding RNAs are key factors in glioma pathogenesis. qRT-PCR analysis was used to assess the expression of FTX and miR-342-3p in the different stages of gliomas in tissues. Bioinformatics tool DIANA and TargetSCan were used to predict the targets of FTX and miR-342-3p, respectively. Pearson's correlation analysis was performed to test the correlation between the expression levels of FTX, miR-342-3p, and astrocyte-elevated gene-1 (AEG-1). To examine the role of FTX in regulating proliferation and invasion of glioma cells, specific siRNA was used to knockdown FTX, and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and transwell assays were performed. Furthermore, rescue experiments were performed to further confirm the regulation of miR-342-3p by FTX. We then found that the expression of FTX and miR-342-3p was associated with progression of gliomas. FTX directly inhibited the expression of miR-342-3p, which subsequently regulates the expression of AEG-1. Collectively, FTX is critical for proliferation and invasion of glioma cells by regulating miR-342-3p and AEG-1. Our findings indicate that FTX and miR-342-3p may serve as a biomarker of glioma diagnosis, and offer potential novel therapeutic targets of treatment of gliomas.
authors.affiliation: Department of Neurosurgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Weiguang
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Shanghai First People's Hospital of Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Yunke
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Department of Neurosurgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Peng
authors.affiliation: Department of Neurosurgery, The 242 Hospital of Harbin, Harbin, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Chenguang
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Laizang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Fubin
authors.initials: F
authors.lastname: Ren
authors.affiliation: Department of Neurosurgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Xie
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Pengwei
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Liang
authors.affiliation: Department of Neurosurgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D008565:Membrane Proteins; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D062085:RNA, Long Noncoding / Q000235:genetics*; D016601:RNA-Binding Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014183:Transplantation, Heterologous; D047368:Tumor Burden / Q000235:genetics; D015854:Up-Regulation
chemical_list: D015815:Cell Adhesion Molecules; C546176:MIRN342 microRNA, human; C483039:MTDH protein, human; D008565:Membrane Proteins; D035683:MicroRNAs; D062085:RNA, Long Noncoding; D016601:RNA-Binding Proteins; C000620866:long non-coding RNA FTX, human