title: The association between chronic pancreatitis and the iNOS-2087A>G polymorphism.
abstract: BACKGROUND
  Chronic pancreatitis is morphologically characterized by ductal dysplasia, breeding grounds for the proliferation of the ductal cells, the degenerative changes in pancreatic acinar cells and fibrosis, and it is defined on the basis of the clinical, morphological and functional criteria.
  
  
  OBJECTIVE
  The aim of our study is to examine the existence of a possible correlation between the iNOS-2087A>G polymorphism and chronic pancreatitis by means of the genetic analysis.
  
  
  METHODS
  We have conducted the study at the Gastroenterology Clinic and the Research Center of Gastroenterology and Hepatology of the University of Medicine and Pharmacy, Craiova, between March 2015 - September 2016. The study had a prospective character. Both for the 58 patients diagnosed with chronic pancreatitis and for the 132 patients in the witness group, the biological material was represented by blood, (around 2.5 - 5 milliliters of venous blood) let on EDTA and kept at 4°C up to the separation of the DNA molecule. All the patients were genotyped for the iNOS - 2087A>G polymorphism, by means of the Real Time PCR technique with TaqMan probes.
  
  
  RESULTS
  Analysing the prevalence of the iNOS genotypes within the study group and witness group, we have noticed that, statistically speaking, there are no significant differences between the two groups.
  
  
  CONCLUSIONS
  As a conclusion, in the study lot we can sustain that the risk of developing chronic pancreatitis is not increased by the presence of the iNOS-2087A>G polymorphism.
authors.affiliation: University of Medicine and Pharmacy Craiova, Craiova, Romania.
authors.forename: Vlad
authors.initials: V
authors.lastname: Pădureanu
authors.affiliation: University of Medicine and Pharmacy Craiova, Craiova, Romania.
authors.forename: Anca Ştefania
authors.initials: AŞ
authors.lastname: Enescu
authors.affiliation: University of Medicine and Pharmacy Craiova, Craiova, Romania.
authors.forename: Isabela
authors.initials: I
authors.lastname: Siloşi
authors.affiliation: University of Medicine and Pharmacy Craiova, Craiova, Romania.
authors.forename: Maria
authors.initials: M
authors.lastname: Forţofoiu
authors.affiliation: University of Medicine and Pharmacy Craiova, Craiova, Romania.
authors.forename: Aurelia
authors.initials: A
authors.lastname: Enescu
authors.affiliation: University of Medicine and Pharmacy Craiova, Craiova, Romania.
authors.forename: Maria
authors.initials: M
authors.lastname: Bogdan
authors.affiliation: University of Medicine and Pharmacy Craiova, Craiova, Romania.
authors.forename: Mircea Cătălin
authors.initials: MC
authors.lastname: Forţofoiu
authors.affiliation: University of Medicine and Pharmacy Craiova, Craiova, Romania.
authors.forename: Amelia Genunche
authors.initials: AG
authors.lastname: Dumitrescu
authors.affiliation: University of Medicine and Pharmacy Craiova, Craiova, Romania.
authors.forename: Diana Rodica
authors.initials: DR
authors.lastname: Tudoraşcu
authors.affiliation: University of Medicine and Pharmacy Craiova, Craiova, Romania.
authors.forename: Adrian
authors.initials: A
authors.lastname: Mita
authors.affiliation: University of Medicine and Pharmacy Craiova, Craiova, Romania.
authors.forename: Ioana
authors.initials: I
authors.lastname: Streata
authors.affiliation: University of Medicine and Pharmacy Craiova, Craiova, Romania.
authors.forename: Mihai
authors.initials: M
authors.lastname: Ioana
authors.affiliation: University of Medicine and Pharmacy Craiova, Craiova, Romania.
authors.forename: Florin
authors.initials: F
authors.lastname: Petrescu
authors.affiliation: University of Medicine and Pharmacy Craiova, Craiova, Romania.
authors.forename: Adrian
authors.initials: A
authors.lastname: Săftoiu
mesh_terms: D016022:Case-Control Studies; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D052247:Nitric Oxide Synthase Type II / Q000235:genetics*; D050500:Pancreatitis, Chronic / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011446:Prospective Studies; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II
keywords: alcohol; chronic pancreatitis; genetic; genotype; iNOS-2087A>G polymorphism