title: Cyclobacterium sediminis sp. nov. isolated from a sea cucumber aquaculture farm and emended description of the genus Cyclobacterium.
abstract: An aerobic, Gram-negative bacterium, designated strain SD70T, was isolated from sea cucumber aquaculture farm sediment in Taean, Korea, and its taxonomic status was established by undertaking a polyphasic study. Cells of strain SD70Twere non-motile, catalase-, and oxidase-positive, non-spore-forming, and horseshoe-shaped. Optimal growth was observed under 25-30°C, pH 7.0-8.0, and 3.0-5.0% (w/v) NaCl conditions. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain SD70Tfell within an evolutionary group comprising species of the genus Cyclobacterium. Strain SD70Tshared 92.1-98.5% 16S rRNA sequence similarity values with the type strains of species of the genus Cyclobacterium. Relatively low levels of DNA-DNA relatedness were found between strain SD70Tand C. marinum DSM 745T(40.2%) and C. amurskyense KMM 6143T(15.8%). The predominant cellular fatty acids were iso-C15:0(32.1%), and anteiso-C15:0(9.1%). Menaquinone MK-7 was the only respiratory quinone. The G+C content of the genomic DNA was 36 mol%. The polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, and seven unidentified lipids. On the basis of phenotypic and genotypic data, strain SD70Trepresents a novel species of the genus Cyclobacterium, for which the name Cyclobacterium sediminis sp. nov. is proposed. An emended description of the genus Cyclobacterium is also provided.
authors.affiliation: Department of Environmental Education, Sunchon National University, Suncheon, 57922, Republic of Korea.
authors.forename: Seyeon
authors.initials: S
authors.lastname: Shin
authors.affiliation: Department of Environmental Education, Sunchon National University, Suncheon, 57922, Republic of Korea. kahng@sunchon.ac.kr.|Research Institute for Sanitation and Environment of Coastal Areas, Sunchon National University, Suncheon, 57922, Republic of Korea. kahng@sunchon.ac.kr.
authors.forename: Hyung-Yeel
authors.initials: HY
authors.lastname: Kahng
mesh_terms: D000818:Animals; D017756:Aquaculture; D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology; D010802:Phylogeny; D056910:Republic of Korea; D012616:Sea Cucumbers / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D024482:Vitamin K 2
keywords: Cyclobacterium sediminis sp. nov.; aquaculture farm sediment; polyphasic study; sea cucumber