title: Building ProteomeTools based on a complete synthetic human proteome.
abstract: We describe ProteomeTools, a project building molecular and digital tools from the human proteome to facilitate biomedical research. Here we report the generation and multimodal liquid chromatography-tandem mass spectrometry analysis of >330,000 synthetic tryptic peptides representing essentially all canonical human gene products, and we exemplify the utility of these data in several applications. The resource (available at http://www.proteometools.org) will be extended to >1 million peptides, and all data will be shared with the community via ProteomicsDB and ProteomeXchange.
authors.affiliation: Chair of Proteomics and Bioanalytics, Technical University of Munich, Freising, Germany.
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Zolg
authors.affiliation: Chair of Proteomics and Bioanalytics, Technical University of Munich, Freising, Germany.
authors.forename: Mathias
authors.initials: M
authors.lastname: Wilhelm
authors.affiliation: JPT Peptide Technologies GmbH, Berlin, Germany.
authors.forename: Karsten
authors.initials: K
authors.lastname: Schnatbaum
authors.affiliation: JPT Peptide Technologies GmbH, Berlin, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Zerweck
authors.affiliation: JPT Peptide Technologies GmbH, Berlin, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Knaute
authors.affiliation: Thermo Fisher Scientific, Bremen, Germany.
authors.forename: Bernard
authors.initials: B
authors.lastname: Delanghe
authors.affiliation: Thermo Fisher Scientific, San Jose, California, USA.
authors.forename: Derek J
authors.initials: DJ
authors.lastname: Bailey
authors.affiliation: Chair of Proteomics and Bioanalytics, Technical University of Munich, Freising, Germany.|SAP SE, Potsdam, Germany.
authors.forename: Siegfried
authors.initials: S
authors.lastname: Gessulat
authors.affiliation: SAP SE, Potsdam, Germany.
authors.forename: Hans-Christian
authors.initials: HC
authors.lastname: Ehrlich
authors.affiliation: Chair of Proteomics and Bioanalytics, Technical University of Munich, Freising, Germany.
authors.forename: Maximilian
authors.initials: M
authors.lastname: Weininger
authors.affiliation: Chair of Proteomics and Bioanalytics, Technical University of Munich, Freising, Germany.
authors.forename: Peng
authors.initials: P
authors.lastname: Yu
authors.affiliation: SAP SE, Walldorf, Germany.
authors.forename: Judith
authors.initials: J
authors.lastname: Schlegl
authors.affiliation: Chair of Proteomics and Bioanalytics, Technical University of Munich, Freising, Germany.
authors.forename: Karl
authors.initials: K
authors.lastname: Kramer
authors.affiliation: Chair of Proteomics and Bioanalytics, Technical University of Munich, Freising, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Schmidt
authors.affiliation: Institute for Systems Biology, Seattle, Washington, USA.
authors.forename: Ulrike
authors.initials: U
authors.lastname: Kusebauch
authors.affiliation: Institute for Systems Biology, Seattle, Washington, USA.
authors.forename: Eric W
authors.initials: EW
authors.lastname: Deutsch
authors.affiliation: Department of Biology, Institute of Molecular Systems Biology, ETH, Zürich, Switzerland.|Faculty of Science, University of Zürich, Zürich, Switzerland.
authors.forename: Ruedi
authors.initials: R
authors.lastname: Aebersold
authors.affiliation: Institute for Systems Biology, Seattle, Washington, USA.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Moritz
authors.affiliation: JPT Peptide Technologies GmbH, Berlin, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Wenschuh
authors.affiliation: Thermo Fisher Scientific, Bremen, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Moehring
authors.affiliation: SAP SE, Potsdam, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Aiche
authors.affiliation: Thermo Fisher Scientific, San Jose, California, USA.
authors.forename: Andreas
authors.initials: A
authors.lastname: Huhmer
authors.affiliation: JPT Peptide Technologies GmbH, Berlin, Germany.
authors.forename: Ulf
authors.initials: U
authors.lastname: Reimer
authors.affiliation: Chair of Proteomics and Bioanalytics, Technical University of Munich, Freising, Germany.|Center for Integrated Protein Science Munich, Freising, Germany.|Bavarian Center for Biomolecular Mass Spectrometry, Freising, Germany.
authors.forename: Bernhard
authors.identifier: 0000-0002-9094-1677
authors.initials: B
authors.lastname: Kuster
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D030562:Databases, Protein; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D020543:Proteome