title: miRNA-451a Targets IFN Regulatory Factor 8 for the Progression of Systemic Lupus Erythematosus.
abstract: Increasing evidence has shown that miRNA-451a (miR-451a) is associated with the development of systemic lupus erythematosus (SLE); however, the mechanism of this association is not fully known. The present study found an increased expression of miR-451a in the spleen and thymus of an SLE mice model. A decrease in miR-451a expression partly relieved the enlargement of the spleen and decreased the proteinuria content and immune complex deposits. The deficiency in miR-451a also decreased numbers of CD4+CD69+ and CD4+/CD8+ T cells and the levels of the serum cytokines IL-17a and IL-4. The IFN regulatory factor (IRF) 8 was highly expressed in the immune organs of wild-type mice but was suppressed in SLE-like mice. A dual-luciferase reporter assay was carried out in combination with gene silencing and overexpression to verify that IRF8 was a target of miR-451a in vitro and in vivo. The data indicate the function and a target of miR-451a in SLE, providing a new target for SLE therapy.
authors.affiliation: Department of Rheumatology & Immunology, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, No.32 West Second Section First Ring Road, Chengdu, 610072, Sichuan, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Department of Rheumatology & Immunology, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, No.32 West Second Section First Ring Road, Chengdu, 610072, Sichuan, China. ruiwurw@163.com.
authors.forename: Rui
authors.initials: R
authors.lastname: Wu
authors.affiliation: Department of Rheumatology & Immunology, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, No.32 West Second Section First Ring Road, Chengdu, 610072, Sichuan, China.
authors.forename: Li
authors.initials: L
authors.lastname: Long
authors.affiliation: Department of Rheumatology & Immunology, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, No.32 West Second Section First Ring Road, Chengdu, 610072, Sichuan, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Su
authors.affiliation: Department of Rheumatology & Immunology, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, No.32 West Second Section First Ring Road, Chengdu, 610072, Sichuan, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Rheumatology & Immunology, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, No.32 West Second Section First Ring Road, Chengdu, 610072, Sichuan, China.
authors.forename: Xiao-Dan
authors.initials: XD
authors.lastname: Wu
authors.affiliation: Department of Rheumatology & Immunology, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, No.32 West Second Section First Ring Road, Chengdu, 610072, Sichuan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Rheumatology & Immunology, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, No.32 West Second Section First Ring Road, Chengdu, 610072, Sichuan, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D018450:Disease Progression; D005786:Gene Expression Regulation / Q000276:immunology; D050835:Interferon Regulatory Factors / Q000032:analysis* / Q000502:physiology; D008180:Lupus Erythematosus, Systemic / Q000209:etiology* / Q000473:pathology; D051379:Mice; D035683:MicroRNAs / Q000032:analysis* / Q000502:physiology; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D013950:Thymus Gland / Q000276:immunology / Q000378:metabolism; D000099185:Interferon Regulatory Factor-8
chemical_list: D015703:Antigens, CD; D016207:Cytokines; D050835:Interferon Regulatory Factors; D035683:MicroRNAs; D000099185:Interferon Regulatory Factor-8; C550758:Mirn451 microRNA, mouse
keywords: IFN regulatory factor 8; cytokines; immune complex deposit; miRNA-451a; systemic lupus erythematosus