title: Functional Peptides from Laminin-1 Improve the Cell Adhesion Capacity of Recombinant Mussel Adhesive Protein.
abstract: Since cell adhesion is important for cell processes such as migration and proliferation, it is a crucial consideration in biomaterial design and development. Based on the fusion of mussel adhesive protein fp151 with laminin-1-originated functional peptides we designed fusion proteins (fLA4, fLG6 and fAG73) and explored their cell adhesion properties. In our study, cell adhesion analysis showed that protein fLG6 and fLA4 had a significantly higher cell adhesion property for A549 than fp151. Moreover, protein fAG73 also displayed a strong adhesion capacity for Hela cells. In conclusion, the incorporation of functional peptides with integrin and heparin/heparan sulphate binding capacity into mussel adhesive protein will promote the application of mussel adhesive protein as cell adhesion biomaterial.
authors.affiliation: The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, 210023. China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, 210023. China.
authors.forename: Lina
authors.initials: L
authors.lastname: Ji
authors.affiliation: The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, 210023. China.
authors.forename: Zichun
authors.initials: Z
authors.lastname: Hua
mesh_terms: D000072283:A549 Cells; D002448:Cell Adhesion / Q000502:physiology*; D004926:Escherichia coli; D006367:HeLa Cells; D006801:Humans; D007797:Laminin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D007797:Laminin; D010446:Peptide Fragments; D011506:Proteins; D011993:Recombinant Fusion Proteins; C037403:adhesive protein, mussel; C121346:laminin 1
keywords: Cell adhesion materials; functional peptides; heparin/heparan sulfate binding peptide; integrin binding peptides; mussel adhesive protein