title: Physiological Analysis of Brassinosteroid Responses and Sensitivity in Rice.
abstract: Identification of related mutants contributed a lot to our understanding of the molecular mechanisms underlying brassinosteroid (BR) signaling. Establishment of methods to test for the hormone sensitivity is essential for the characterization of the mutants associated with BR responses. Here, we describe detailed procedures for the physiological analysis of BR responses that are regularly applied to test the BR sensitivity of mutants by evaluating the growth of different tissues of rice (Oryza sativa) seedlings.
authors.affiliation: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, No.1 West Beichen Road, Chaoyang District, Beijing, 100101, China.
authors.forename: Hongning
authors.initials: H
authors.lastname: Tong
authors.affiliation: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, No.1 West Beichen Road, Chaoyang District, Beijing, 100101, China. ccchu@genetics.ac.cn.
authors.forename: Chengcai
authors.initials: C
authors.lastname: Chu
mesh_terms: D060406:Brassinosteroids / Q000494:pharmacology*; D018548:Cotyledon / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D009154:Mutation; D012275:Oryza / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D015398:Signal Transduction; D013260:Steroids, Heterocyclic / Q000494:pharmacology*
chemical_list: D060406:Brassinosteroids; D010937:Plant Growth Regulators; D010940:Plant Proteins; D013260:Steroids, Heterocyclic; C023623:brassinolide
keywords: Brassinosteroid response; Brassinosteroid sensitivity; Coleoptile elongation; Lamina inclination; Rice