title: Diversity of culturable bacterial communities in the intestinal tracts of goldfish (Carassius auratus) and their ability to produce N-acyl homoserine lactone.
abstract: Intestinal bacteria isolated from goldfish (Carassius auratus) were identified based on 16 ribosomal RNA (rRNA) gene sequences and screened for their ability to produce N-acyl homoserine lactone (AHL), an autoinducer of the quorum sensing (QS) system. The 230 aerobes/facultative anaerobes that were isolated comprised members of the genera Aeromonas (184 isolates), Citrobacter (11), Enterobacter (2), Shewanella (28), Vagococcus (1), and Vibrio (4). Among these genera, the two most abundant species were Aeromonas veronii (163 isolates) and Shewanella xiamenensis (27). In addition, 142 obligate anaerobes consisting of Cetobacterium somerae (139 isolates), Clostridium frigidicarnis (2), and Cetobacterium sp. (1) were also isolated. One hundred seventy isolates (74.2%) belonging to the genera Aeromonas, Citrobacter, Enterobacter, Shewanella, and Vibrio produced AHL, while 155 (67.7%) and 91 (39.7%) isolates possessed the luxR and luxI gene homologs, respectively. None of the obligate anaerobes produced AHL or possessed luxRI homologs. Total viable counts ranged from 1.2 × 107to 2.2 × 109CFU/g, which were accounted for 0.8 to 15.2% of direct counts. Aeromonas veronii, S. xiamenensis, and C. somerae were detected from five goldfish at densities ranging from 4.0 × 106to 1.7 × 109CFU/g, indicating that these bacteria are dominant components of the culturable gut flora in goldfish. In addition, members of the genera Aeromonas and Shewanella appeared to communicate with each other by using the QS system to some extent when the concentration of AHL reaches a certain threshold. It is therefore suggested that bacteria with the ability to disrupt AHL secretion in intestinal environments are potential candidates for probionts for preventing opportunistic infections in freshwater fish such as goldfish.
authors.affiliation: Department of Marine Science and Resources, Nihon University, Kameino 1866, Fujisawa, 252-0880, Kanagawa, Japan. sugita.haruo@nihon-u.ac.jp.
authors.forename: Haruo
authors.initials: H
authors.lastname: Sugita
authors.affiliation: Department of Marine Science and Resources, Nihon University, Kameino 1866, Fujisawa, 252-0880, Kanagawa, Japan.
authors.forename: Shun
authors.initials: S
authors.lastname: Kitao
authors.affiliation: Department of Marine Science and Resources, Nihon University, Kameino 1866, Fujisawa, 252-0880, Kanagawa, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Narisawa
authors.affiliation: Department of Marine Science and Resources, Nihon University, Kameino 1866, Fujisawa, 252-0880, Kanagawa, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Minamishima
authors.affiliation: Department of Marine Science and Resources, Nihon University, Kameino 1866, Fujisawa, 252-0880, Kanagawa, Japan.
authors.forename: Shiro
authors.initials: S
authors.lastname: Itoi
mesh_terms: D054742:Acyl-Butyrolactones / Q000378:metabolism*; D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000378:metabolism*; D058448:Biota; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D041981:Gastrointestinal Tract / Q000382:microbiology*; D006054:Goldfish / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D054742:Acyl-Butyrolactones; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: AHL; Aeromonas; Cetobacterium; Quorum sensing; Shewanella; goldfish; gut flora; intestinal bacteria