title: Inoculating wheat (Triticum aestivum L.) with the endophytic bacterium Serratia sp. PW7 to reduce pyrene contamination.
abstract: This research was conducted to find an optimal inoculation way for a pyrene-degrading endophytic Serratia sp. PW7 to colonize wheat for reducing pyrene contamination. Three inoculation ways, which are soaking seeds in inocula (TS), dipping roots of seedlings in inocula (TR), and spraying inocula on leaves of seedlings (TL), were used in this study. Inoculated seedlings and noninoculated seedlings (CK) were, respectively, cultivated in Hoagland solutions supplemented with pyrene in a growth chamber. The results showed that strain PW7 successfully colonized the inoculated seedlings in high numbers, and significantly promoted the growth of seedlings (TS and TR). More importantly, strain PW7 reduced pyrene levels in the seedlings and the Hoagland solutions. Compared to the noninoculated seedlings, the pyrene contents of the inoculated seedlings were decreased by 35.7-86.3% in the shoots and by 26.8-60.1% in the roots after 8-day cultivation. By comparing the efficiencies of decreasing pyrene residues, it can be concluded that TR was an optimal inoculation way for endophytic strains to colonize the inoculated plants and to reduce the pyrene contamination. Our findings provide an optimized inoculation way to reduce organic contamination in crops by inoculating plants with functional endophytic bacteria.
authors.affiliation: a Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University , Nanjing , P.R. China.
authors.forename: Xuezhu
authors.initials: X
authors.lastname: Zhu
authors.affiliation: a Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University , Nanjing , P.R. China.
authors.forename: Wanqing
authors.initials: W
authors.lastname: Wang
authors.affiliation: a Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University , Nanjing , P.R. China.
authors.forename: Kai
authors.initials: K
authors.lastname: Sun
authors.affiliation: a Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University , Nanjing , P.R. China.
authors.forename: Xianghao
authors.initials: X
authors.lastname: Lin
authors.affiliation: a Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University , Nanjing , P.R. China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Li
authors.affiliation: a Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University , Nanjing , P.R. China.
authors.forename: Michael Gatheru
authors.initials: MG
authors.lastname: Waigi
authors.affiliation: a Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University , Nanjing , P.R. China.
authors.forename: Wanting
authors.initials: W
authors.lastname: Ling
mesh_terms: D001673:Biodegradation, Environmental; D018517:Plant Roots; D011721:Pyrenes / Q000378:metabolism*; D036226:Seedlings; D012705:Serratia; D012989:Soil Pollutants / Q000378:metabolism*; D014908:Triticum / Q000382:microbiology*
chemical_list: D011721:Pyrenes; D012989:Soil Pollutants
keywords: bioremediation; colonization efficiencies; function endophytic bacterium; inoculation way; pyrene