title: High Molecular Weight DNA Enrichment with Peptide Nucleic Acid Probes.
abstract: Here, we describe how peptide nucleic acid (PNA) probes can be used to enrich genomic DNA fractions to facilitate downstream analysis, such as the haplotype phasing of the isolated genomic pieces. This method enriches for polymorphic regions of fragmented chromosomes by physically separating the desired sequence and flanking regions. The PNA probes used for enrichment are novel synthetic nucleic acids with highly specific targeting and hybridization properties. Using a enrichment technique, we capture high molecular weight genomic DNA using nothing more than a simple modification to standard genomic DNA extraction from blood.
authors.affiliation: Monash Institute of Pharmaceutical Science, Monash University, 381 Royal Parade, Parkville, VIC, 3052, Australia. nickmurphy@monash.edu.|Preimplantation Genetic Diagnosis, Monash IVF, Melbourne, VIC, Australia. nickmurphy@monash.edu.
authors.forename: Nicholas M
authors.initials: NM
authors.lastname: Murphy
authors.affiliation: Monash Institute of Pharmaceutical Science, Monash University, 381 Royal Parade, Parkville, VIC, 3052, Australia.
authors.forename: Colin W
authors.initials: CW
authors.lastname: Pouton
authors.affiliation: Monash Institute of Pharmaceutical Science, Monash University, 381 Royal Parade, Parkville, VIC, 3052, Australia. helen.irving@monash.edu.
authors.forename: Helen R
authors.initials: HR
authors.lastname: Irving
mesh_terms: D006680:HLA Antigens / Q000235:genetics; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D008970:Molecular Weight; D015341:Nucleic Acid Probes; D020135:Peptide Nucleic Acids / Q000235:genetics*
chemical_list: D006680:HLA Antigens; D015341:Nucleic Acid Probes; D020135:Peptide Nucleic Acids
keywords: Haplotype enrichment; High molecular weight DNA; Human leukocyte antigen system alleles; Major histocompatibility complex; Peptide nucleic acid probes