title: Pannexin1 Single Nucleotide Polymorphism and Platelet Reactivity in a Cohort of Cardiovascular Patients.
abstract: Pannexin1 (Panx1), a membrane channel-forming protein permitting the passage of small-sized molecules, such as ATP, is expressed in human platelets. Recently, we showed that inhibiting Panx1 affects collagen-induced platelet aggregation but not aggregation triggered by other agonists. We also found that a single nucleotide polymorphism (SNP; rs1138800) in the Panx1 gene encoded for a gain-of-function channel (Panx1-400C) and was associated with enhanced collagen-induced platelet reactivity. Here, we assessed the association of this SNP with platelet reactivity in a cohort of 758 stable cardiovascular patients from the ADRIE study treated with aspirin and/or clopidogrel. We found that presence of the Panx1-400C allele was not associated with platelet reactivity in stable cardiovascular patients, irrespective of the platelet aggregation agonist used (collagen, ADP or arachidonic acid) or the anti-platelet drug regimen. Moreover, the Panx1-400A > C SNP did also not affect the re-occurrence of cardiac ischemic events in the same stable cardiovascular patient cohort.
authors.affiliation: a Department of Pathology and Immunology , University of Geneva , Geneva , Switzerland.|b Department of Medical Specializations - Cardiology , University of Geneva , Geneva , Switzerland.|c Geneva Platelet Group , University of Geneva , Geneva , Switzerland.
authors.forename: Florian B
authors.initials: FB
authors.lastname: Stierlin
authors.affiliation: a Department of Pathology and Immunology , University of Geneva , Geneva , Switzerland.|b Department of Medical Specializations - Cardiology , University of Geneva , Geneva , Switzerland.
authors.forename: Filippo
authors.initials: F
authors.lastname: Molica
authors.affiliation: c Geneva Platelet Group , University of Geneva , Geneva , Switzerland.|d Department of Medical Specializations, Division of Internal Medicine and Rehabilitation , Trois-Chêne Hospital, Geneva University Hospitals , Geneva , Switzerland.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Reny
authors.affiliation: a Department of Pathology and Immunology , University of Geneva , Geneva , Switzerland.|b Department of Medical Specializations - Cardiology , University of Geneva , Geneva , Switzerland.
authors.forename: Brenda R
authors.initials: BR
authors.lastname: Kwak
authors.affiliation: c Geneva Platelet Group , University of Geneva , Geneva , Switzerland.|e Department of Medical Specializations, Division of Angiology and Haemostasis , Geneva University Hospitals , Geneva , Switzerland.
authors.forename: Pierre
authors.initials: P
authors.lastname: Fontana
mesh_terms: D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002318:Cardiovascular Diseases / Q000097:blood* / Q000235:genetics*; D015331:Cohort Studies; D003094:Collagen / Q000494:pharmacology; D017630:Connexins / Q000235:genetics*; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D009419:Nerve Tissue Proteins / Q000235:genetics*; D010974:Platelet Aggregation / Q000187:drug effects; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D017630:Connexins; D009419:Nerve Tissue Proteins; C492764:PANX1 protein, human; D003094:Collagen
keywords: Platelet aggregation; anti-platelet drugs; collagen; genetic polymorphism; membrane channel; pannexin1