title: Fabrication of Novel Hydrogel with Berberine-Enriched Carboxymethylcellulose and Hyaluronic Acid as an Anti-Inflammatory Barrier Membrane.
abstract: An antiadhesion barrier membrane is an important biomaterial for protecting tissue from postsurgical complications. However, there is room to improve these membranes. Recently, carboxymethylcellulose (CMC) incorporated with hyaluronic acid (HA) as an antiadhesion barrier membrane and drug delivery system has been reported to provide excellent tissue regeneration and biocompatibility. The aim of this study was to fabricate a novel hydrogel membrane composed of berberine-enriched CMC prepared from bark of theP. amurensetree and HA (PE-CMC/HA). In vitro anti-inflammatory properties were evaluated to determine possible clinical applications. The PE-CMC/HA membranes were fabricated by mixing PE-CMC and HA as a base with the addition of polyvinyl alcohol to form a film. Tensile strength and ultramorphology of the membrane were evaluated using a universal testing machine and scanning electron microscope, respectively. Berberine content of the membrane was confirmed using a UV-Vis spectrophotometer at a wavelength of 260 nm. Anti-inflammatory property of the membrane was measured using a Griess reaction assay. Our results showed that fabricated PE-CMC/HA releases berberine at a concentration of 660μg/ml while optimal plasticity was obtained at a 30 : 70 PE-CMC/HA ratio. The berberine-enriched PE-CMC/HA had an inhibited 60% of inflammation stimulated by LPS. These results suggest that the PE-CMC/HA membrane fabricated in this study is a useful anti-inflammatory berberine release system.
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
authors.forename: Yu-Chih
authors.initials: YC
authors.lastname: Huang
authors.affiliation: Graduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei, Taiwan.
authors.forename: Kuen-Yu
authors.initials: KY
authors.lastname: Huang
authors.affiliation: School of Forestry and Resource Conservation, National Taiwan University, Taipei, Taiwan.
authors.forename: Bing-Yuan
authors.initials: BY
authors.lastname: Yang
authors.affiliation: School of Forestry and Resource Conservation, National Taiwan University, Taipei, Taiwan.
authors.forename: Chun-Han
authors.identifier: 0000-0002-8112-3381
authors.initials: CH
authors.lastname: Ko
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan; Graduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei, Taiwan; Graduate Institute of Biomedical Optomechatronics, College of Biomedical Engineering, Taipei Medical University, Taipei, Taiwan.
authors.forename: Haw-Ming
authors.identifier: 0000-0003-4184-3744
authors.initials: HM
authors.lastname: Huang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000737:chemistry*; D001599:Berberine / Q000737:chemistry*; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000737:chemistry*; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry; D002460:Cell Line; D016503:Drug Delivery Systems / Q000379:methods; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000008:administration & dosage / Q000737:chemistry*; D008567:Membranes, Artificial; D051379:Mice; D041681:NIH 3T3 Cells; D011183:Postoperative Complications / Q000517:prevention & control; D013718:Tensile Strength; D000267:Tissue Adhesions / Q000188:drug therapy; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000893:Anti-Inflammatory Agents; D001672:Biocompatible Materials; D008567:Membranes, Artificial; D001599:Berberine; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D006820:Hyaluronic Acid; D002266:Carboxymethylcellulose Sodium