title: A novel long noncoding RNA PILRLS promote proliferation through TCL1A by activing MDM2 in Retroperitoneal liposarcoma.
abstract: It is becoming evident that lncRNAs may be an important class of pervasive genes involved in carcinogenesis and metastasis. However, the biological and molecular mechanisms of lncRNAs in retroperitoneal liposarcoma have never been reported. In our study, we found a novel lncRNA PILRLS (Proliferation Interacting LncRNA in Retroperitoneal Liposarcoma), which as an oncogene significantly overexpressed in retroperitoneal liposarcoma. Functions of PILRLS on tumor progression both in vitro and in vivo have verified in this study which PILRLS knockdown significantly inhibited cell proliferation and colony formation. RNA pull-down assay found PILRLS can specific binding with TCL1A which also regulate the expression level of TCL1A. Our work for the first time demonstrated PILRLS can activating the MDM2 by binding with TCL1A which suppress the P53 pathway to promote the unlimited growth of retroperitoneal Liposarcoma cells. It suggests that PILRLS may be an important targets for retroperitoneal liposarcoma therapy.
authors.affiliation: Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Yebo
authors.initials: Y
authors.lastname: Shao
authors.affiliation: Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Medical Pathology, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Yingyong
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Hanxing
authors.initials: H
authors.lastname: Tong
authors.affiliation: Department of Medical Oncology, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Rongyuan
authors.initials: R
authors.lastname: Zhuang
authors.affiliation: Department of Ultrasound, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Zhengbiao
authors.initials: Z
authors.lastname: Ji
authors.affiliation: Department of Radiation Oncology, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Binliang
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Medical Oncology, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Weiqi
authors.initials: W
authors.lastname: Lu
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D064593:Heterografts; D006801:Humans; D008080:Liposarcoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D051736:Proto-Oncogene Proteins c-mdm2 / Q000235:genetics / Q000378:metabolism*; D062085:RNA, Long Noncoding; D060888:Real-Time Polymerase Chain Reaction; D012186:Retroperitoneal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*
chemical_list: D011518:Proto-Oncogene Proteins; D062085:RNA, Long Noncoding; C090851:TCL1A protein, human; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2
keywords: LncRNA-PILRLS; MDM2; P53 signaling pathway; proliferation; retroperitoneal liposarcoma