title: Inhibitory effects of mouse bone marrow mesenchymal stem cell soup on staurospurine-induced cell death in MCF-7 and AGS.
abstract: Staurospurine induces apoptosis in cell line. Bone Marrow Mesenchymal stem cells Soup is a promising tool for cell proliferation via a variety of secreted factors. In this study, we examined the effects of BMSCs Soup on Staurospurine induced-cell death in MCF-7 and AGS cells.
  
  
  
  There were three Groups: Group I: no incubation with BM Soup; Group II: incubated with 24 h BM Soup; Group III: incubation with 48 h BM Soup. There were two treatments in each group. The treatments were 1μM Staurospurine (Treatment 1) and 0.0 μM Staurospurine (Treatment 2). The cells were cultured in culture medium containing 0.2 % BSA. We obtained the cell viability, cell death and NO concentration.
  
  
  
  Our results showed that BM soup administration for 48 hours protectsed against 1μM staurosporine concentration induced cell death and reduced cell toxicity in MCF-7 and AGS cells. Cell viability and cell toxicity assay showed that BM soup in time dependent manner increased cell viability (p < 0.05) and cell death assay showed that cell death in time dependent manner was decreased(p < 0.05). Our data showed that BM soup with increasing NO concentration reduced staurospurine induced cell death and cell cytotoxicity (p < 0.05).
  
  
  
  It's concluded that BMSCs soup suppressed staurospurine-induced cytotoxicity activity process in MCF-7 and AGS cells (Fig. 9, Ref. 79).
authors.forename: M
authors.initials: M
authors.lastname: Zhaleh
authors.forename: M
authors.initials: M
authors.lastname: Azadbakht
authors.forename: A
authors.initials: A
authors.lastname: Bidmeshki Pour
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology*; D001854:Bone Marrow Cells / Q000502:physiology*; D016923:Cell Death / Q000187:drug effects* / Q000502:physiology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects* / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D016207:Cytokines / Q000502:physiology*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry / Q000502:physiology*; D061986:MCF-7 Cells; D059630:Mesenchymal Stem Cells / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology*; D051379:Mice; D019311:Staurosporine / Q000494:pharmacology*
chemical_list: D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins; D019311:Staurosporine
keywords: BM-MSC soup; cell death NO.; cell viability