title: Comparative erythrocyte deformability investigations by filtrometry, slit-flow and rotational ektacytometry in a long-term follow-up animal study on splenectomy and different spleen preserving operative techniques: Partial or subtotal spleen resection and spleen autotransplantation.
abstract: BACKGROUND
  Partial or subtotal spleen resection or spleen autotransplantation can partly preserve/restore the splenic filtration function, as previous studies demonstrated.
  
  
  OBJECTIVE
  For better evaluation and follow-up of the various spleen-preserving operative techniques' effectiveness versus splenectomy, a composite methodological approach was applied in a canine experimental model.
  
  
  METHODS
  Beagle dogs were subjected to control (n = 6), splenectomy (SE, n = 4), partial and subtotal spleen resection (n = 4/each) or spleen autotransplantation groups (AU, Furka's spleen-chip method, n = 8). The follow-up period was 18 postoperative (p.o.) months. Erythrocyte deformability was determined in parallel by bulk filtrometry (Carat FT-1 filtrometer), slit-flow ektacytometry (RheoScan D-200) and rotational ektacytometry (LoRRca MaxSis Osmoscan).
  
  
  RESULTS
  By filtrometry, relative cell transit time increased in the SE group (mostly in animal Nr. SE-3), showing the highest values on the 3rd, 9th and in 18th p.o. months. Elongation index values decreased in this group (both by slit-flow and rotational ektacytometers). In general, AU and two resection groups' values were lower versus control and higher than in SE.
  
  
  CONCLUSIONS
  Forasmuch in the circulation both elongation by shear stress and filtration occur, these various erythrocyte deformability testing methods together may describe better the alterations. Considering the possible complications related to functional asplenic-hyposplenic conditions, individual analysis of cases is highly important.
authors.affiliation: Department of Operative Techniques and Surgical Research, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: Iren
authors.initials: I
authors.lastname: Miko
authors.affiliation: Department of Operative Techniques and Surgical Research, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: Norbert
authors.initials: N
authors.lastname: Nemeth
authors.affiliation: Department of Operative Techniques and Surgical Research, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: Viktoria
authors.initials: V
authors.lastname: Sogor
authors.affiliation: Department of Operative Techniques and Surgical Research, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: Ferenc
authors.initials: F
authors.lastname: Kiss
authors.affiliation: Department of Operative Techniques and Surgical Research, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: Eniko
authors.initials: E
authors.lastname: Toth
authors.affiliation: Department of Operative Techniques and Surgical Research, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: Katalin
authors.initials: K
authors.lastname: Peto
authors.affiliation: Department of Clinical Oncology, Division of Radiotherapy, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: Andrea
authors.initials: A
authors.lastname: Furka
authors.affiliation: Department of Operative Techniques and Surgical Research, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: Erzsebet
authors.initials: E
authors.lastname: Vanyolos
authors.affiliation: Department of Pathology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: Laszlo
authors.initials: L
authors.lastname: Toth
authors.affiliation: Department of Nuclear Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: Jozsef
authors.initials: J
authors.lastname: Varga
authors.affiliation: Department of Biophysics and Radiation Biology, Faculty of Medicine, Semmelweis University, Budapest, Hungary.
authors.forename: Krisztian
authors.initials: K
authors.lastname: Szigeti
authors.affiliation: Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: Ilona
authors.initials: I
authors.lastname: Benkő
authors.affiliation: Department of Laboratory Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: Anna V
authors.initials: AV
authors.lastname: Olah
authors.affiliation: Department of Operative Techniques and Surgical Research, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: Istvan
authors.initials: I
authors.lastname: Furka
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D004907:Erythrocyte Deformability / Q000502:physiology*; D005500:Follow-Up Studies; D006801:Humans; D011184:Postoperative Period; D013154:Spleen / Q000473:pathology / Q000601:surgery*; D013156:Splenectomy / Q000379:methods*; D014182:Transplantation, Autologous / Q000379:methods*
keywords: Spleen filtration function; bulk filtrometry; red blood cell deformability; slit flow and rotational ektacytometry; spleen autotransplantation; spleen partial or subtotal resection; splenectomy