title: RAP1B, a DVL2 binding protein, activates Wnt/beta-catenin signaling in esophageal squamous cell carcinoma.
abstract: RAP1B is a small GTPase, which regulates multiple cellular processes. Up-regulation of RAP1B has been observed in several cancer types. Although previous study has shown that miR-518 inhibited the proliferation and invasion of esophageal squamous cell carcinoma (ESCC) cells possibly by targeting RAP1B, the expression pattern and the functions of RAP1B in ESCC are not fully understood. Here, we have fund that the expression of RAP1B was up-regulated in ESCC clinical samples. Gain-of-function and loss-of-function assays demonstrated that RAP1B promoted the growth, migration and metastasis of the ESCC cells. Moreover, the mechanism study showed that RAP1B interacted with DVL2, an important upstream regulator for beta-catenin/TCF signaling, and activated beta-catenin/TCF signaling. Taken together, our study demonstrated the oncogenic roles of RAP1B in ESCC, and suggested that RAP1B might be a therapeutic target.
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital, Zhengzhou University, Henan province, PR China; Department of Key Thoracic Tumour Experimental Laboratory of Zhengzhou, PR China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Jia
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital, Zhengzhou University, Henan province, PR China; Department of Key Thoracic Tumour Experimental Laboratory of Zhengzhou, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital, Zhengzhou University, Henan province, PR China; Department of Key Thoracic Tumour Experimental Laboratory of Zhengzhou, PR China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Dengyan
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital, Zhengzhou University, Henan province, PR China; Department of Key Thoracic Tumour Experimental Laboratory of Zhengzhou, PR China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Chunyang
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital, Zhengzhou University, Henan province, PR China; Department of Key Thoracic Tumour Experimental Laboratory of Zhengzhou, PR China.
authors.forename: Liu
authors.initials: L
authors.lastname: Donglei
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital, Zhengzhou University, Henan province, PR China; Department of Key Thoracic Tumour Experimental Laboratory of Zhengzhou, PR China.
authors.forename: Wu
authors.initials: W
authors.lastname: Kai
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital, Zhengzhou University, Henan province, PR China; Department of Key Thoracic Tumour Experimental Laboratory of Zhengzhou, PR China. Electronic address: zhaosongdoc@aliyun.com.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Song
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D000072261:Dishevelled Proteins / Q000235:genetics / Q000378:metabolism*; D004938:Esophageal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D011485:Protein Binding; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014183:Transplantation, Heterologous; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*; D020668:rap GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C000606581:DVL2 protein, human; D000072261:Dishevelled Proteins; D051176:beta Catenin; C116753:RAP1B protein, human; D020668:rap GTP-Binding Proteins
keywords: Beta-catenin/TCF signaling; Cell growth and migration; ESCC; RAP1B