title: Transcutaneous immunization of Streptococcus suis bacterin using dissolving microneedles.
abstract: Vaccine delivery using microneedle (MN) patches is an easy, safe and painless alternative to traditional needle injections. In this study, we examined whether MN patches can enhance the efficacy of a Streptococcus suis serotype 2 (S. suis 2) vaccine in a mouse model. Results showed that MNs can reach 200-250μm into the skin, a depth beneficial for targeted delivery of antigens to antigen-presenting cells in the epidermis and dermis. Vaccination with prime-boost of MN induced higher levels of IgG2a antibody titer, T cell proliferation, and TH1 cytokines (IFN-γ and IL-12) as compared to intramuscular (IM) injection. In addition, single dose MN vaccination better protected mice against lethal challenge than IM vaccination. MN vaccination also conferred long-term IgG2a antibody against S. suis 2 bacteria presence for up to 7 months. Taken together, these data showed that vaccine delivery by MNs results in superior immune response and protection rate when compared to IM injections.
authors.affiliation: Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, 91201 Taiwan; Graduate Institute of Animal Vaccine Technology, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, 91201 Taiwan.
authors.forename: Kai-Jen
authors.initials: KJ
authors.lastname: Hsueh
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan, 701 Taiwan.
authors.forename: Mei-Chin
authors.initials: MC
authors.lastname: Chen
authors.affiliation: Graduate Institute of Animal Vaccine Technology, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, 91201 Taiwan.
authors.forename: Li-Ting
authors.initials: LT
authors.lastname: Cheng
authors.affiliation: Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology, Pingtung, 91201 Taiwan.
authors.forename: Jai-Wei
authors.initials: JW
authors.lastname: Lee
authors.affiliation: Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, 91201 Taiwan.
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Chung
authors.affiliation: Graduate Institute of Animal Vaccine Technology, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, 91201 Taiwan. Electronic address: cychu@mail.npust.edu.tw.
authors.forename: Chun-Yen
authors.initials: CY
authors.lastname: Chu
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D016207:Cytokines / Q000097:blood / Q000276:immunology; D007115:Immunization Schedule; D007074:Immunoglobulin G / Q000097:blood; D007273:Injections, Intramuscular; D051379:Mice; D008807:Mice, Inbred BALB C; D008845:Microinjections / Q000379:methods; D009339:Needles; D012867:Skin / Q000276:immunology*; D013290:Streptococcal Infections / Q000276:immunology* / Q000517:prevention & control; D022541:Streptococcal Vaccines / Q000008:administration & dosage*; D017011:Streptococcus suis / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D057968:Transdermal Patch; D014611:Vaccination / Q000379:methods*
chemical_list: D016207:Cytokines; D007074:Immunoglobulin G; D022541:Streptococcal Vaccines
keywords: Microneedles (MNs); Streptococcus suis; Transcutaneous immunization