title: Relationship Between Some Single-nucleotide Polymorphism and Response to Hydroxyurea Therapy in Iranian Patients With β-Thalassemia Intermedia.
abstract: To evaluate the possible relationship between hydroxyurea (HU) response and some single-nucleotide polymorphism (SNP) in patients affected by β-thalassemia intermedia.
  
  
  
  In this cross-sectional study, 100 β-thalassemia intermedia patients who were taking HU with a dose of 8 to 15 mg/kg body weight per day for a period of at least 6 months were randomly selected between February 2013 and October 2014 in southern Iran. HU response was defined based on decrease or cessation of the blood transfusion need and evaluation of Hb level.
  
  
  
  In univariate analysis, from all evaluated SNPs, only rs10837814 SNP of olfactory receptors (ORs) OR51B2 showed a significant association with HU response (P=0.038) and from laboratory characteristics, only nucleated red blood cells showed significant associations (116%±183%) in good responders versus (264%±286%) in poor responders (P=0.045). In multiple logistic regression, neither laboratory variables nor different SNPs, showed significant association with HU response. Three novel nucleotide variations (-665 [A→C], -1301 [T→G],-1199 delA) in OR51B2 gene were found in good responders.
  
  
  
  None of the evaluated SNPs in our study showed significant association with HU response. Further larger studies and evaluation of other genes are suggested.
authors.affiliation: *Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran †Shahid Beheshti University of Medical Sciences, Tehran ‡Clinical Pharmacy Department, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz §Clinical Biochemistry Department of Biology Faculty of Sciences, University of Zabol, Zabol, Iran.
authors.forename: Mehran
authors.initials: M
authors.lastname: Karimi
authors.forename: Tahereh
authors.initials: T
authors.lastname: Zarei
authors.forename: Sezaneh
authors.initials: S
authors.lastname: Haghpanah
authors.forename: Mohamad
authors.initials: M
authors.lastname: Moghadam
authors.forename: Ahmad
authors.initials: A
authors.lastname: Ebrahimi
authors.forename: Narges
authors.initials: N
authors.lastname: Rezaei
authors.forename: Ghazaleh
authors.initials: G
authors.lastname: Heidari
authors.forename: Afsaneh
authors.initials: A
authors.lastname: Vazin
authors.forename: Maryam
authors.initials: M
authors.lastname: Khavari
authors.forename: Hamid R
authors.initials: HR
authors.lastname: Miri
mesh_terms: D000293:Adolescent; D000328:Adult; D001803:Blood Transfusion / Q000706:statistics & numerical data; D003430:Cross-Sectional Studies; D005260:Female; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D006918:Hydroxyurea / Q000008:administration & dosage* / Q000494:pharmacology; D007492:Iran; D016015:Logistic Models; D008297:Male; D020641:Polymorphism, Single Nucleotide; D018035:Receptors, Odorant / Q000235:genetics; D016896:Treatment Outcome; D055815:Young Adult; D017086:beta-Thalassemia / Q000188:drug therapy / Q000235:genetics*
chemical_list: D006454:Hemoglobins; D018035:Receptors, Odorant; D006918:Hydroxyurea