title: MicroRNA-22 regulates inflammation and angiogenesis via targeting VE-cadherin.
abstract: The vascular endothelial (VE)-cadherin functions as an endothelial barrier protein controlling endothelial permeability and leukocyte transmigration. Developmental studies indicate that VE-cadherin also plays a vital role in angiogenesis. MicroRNA-22 plays important roles in cardiovascular diseases including cardiac hypertrophy and heart failure. We identified that miR-22 interacts with VE-cadherin mRNA. Overexpression of miR-22 in endothelial cells increases the synthesis of proinflammatory cytokines. Injection of miR-22 results in increased myeloperoxidase activity in the mouse lungs. Moreover, miR-22 injection into the fluorescent-labeled transgenic zebrafish Tg(fli1:EGFP) embryos caused defective vascular development in the dorsal and intersegmental vessels, and vascular markers were significantly suppressed in these embryos. Our studies demonstrate that the conserved targeting of VE-cadherin by miR-22 regulates endothelial inflammation, tissue injury, and angiogenesis.
authors.affiliation: Department of Basic Veterinary Medicine, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gu
authors.affiliation: Department of Basic Veterinary Medicine, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Zhan
authors.affiliation: Key Laboratory of Fresh Water Animal Breeding, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: Xin-Ying
authors.initials: XY
authors.lastname: Zhou
authors.affiliation: Department of Basic Veterinary Medicine, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: Lun
authors.initials: L
authors.lastname: Yao
authors.affiliation: Department of Basic Veterinary Medicine, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: Meiping
authors.initials: M
authors.lastname: Yan
authors.affiliation: Department of Basic Veterinary Medicine, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: Ao
authors.initials: A
authors.lastname: Chen
authors.affiliation: Department of Basic Veterinary Medicine, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Basic Veterinary Medicine, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: Xiaojiao
authors.initials: X
authors.lastname: Ren
authors.affiliation: Department of Basic Veterinary Medicine, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Fresh Water Animal Breeding, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: Jing-Xia
authors.initials: JX
authors.lastname: Liu
authors.affiliation: Department of Basic Veterinary Medicine, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: Guoquan
authors.initials: G
authors.lastname: Liu
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000378:metabolism; D004727:Endothelium / Q000473:pathology; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism; D007249:Inflammation / Q000235:genetics* / Q000473:pathology; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D018919:Neovascularization, Physiologic / Q000235:genetics*; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*; D000099152:Cadherin 5
chemical_list: D020413:3' Untranslated Regions; D015703:Antigens, CD; D015820:Cadherins; D035683:MicroRNAs; D012333:RNA, Messenger; D000099152:Cadherin 5; C548375:MIRN22 microRNA, human; C000619248:MIRN22 microRNA, zebrafish; C574876:Mirn22 microRNA, mouse
keywords: endothelial inflammation; miR-22; vascular development; vascular endothelial-cadherin