title: Biological Treatment of Cyanide by UsingKlebsiella pneumoniaeSpecies.
abstract: In this study, optimization conditions for cyanide biodegradation byKlebsiella pneumoniaestrain were determined to be 25 °C, pH=7 and 150 rpm at the concentration of 0.5 mM potassium cyanide in the medium. Additionally, it was found thatK. pneumoniaestrain is not only able to degrade potassium cyanide, but also to degrade potassium hexacyanoferrate(II) trihydrate and sodium ferrocyanide decahydrate with the efficiencies of 85 and 87.5%, respectively. Furthermore, this strain degraded potassium cyanide in the presence of different ions such as magnesium, nickel, cobalt, iron, chromium, arsenic and zinc, in variable concentrations (0.1, 0.25 and 0.5 mM) and as a result the amount of the bacteria in the biodegradation media decreased with the increase of ion concentration. Lastly, it was also observed that sterile crude extract ofK. pneumoniaestrain degraded potassium cyanide on the fifth day of incubation. Based on these results, it is concluded that both culture and sterile crude extract ofK. pnemoniaewill be used in cyanide removal from different wastes.
authors.affiliation: Hacettepe University, Faculty of Science, Department of Biology (Biotechnology), Beytepe, TR-06800 Ankara, Turkey.
authors.forename: Nermin Hande
authors.initials: NH
authors.lastname: Avcioglu
authors.affiliation: Hacettepe University, Faculty of Science, Department of Biology (Biotechnology), Beytepe, TR-06800 Ankara, Turkey.
authors.forename: Isil Seyis
authors.initials: IS
authors.lastname: Bilkay
keywords: Klebsiella pneumoniae; biodegradation; cyanide