title: Kinase Assay-Linked Phosphoproteomics: Discovery of Direct Kinase Substrates.
abstract: Dissection of direct kinase-substrate relationships provides invaluable information about phosphorylation pathways and can highlight both pathogenic mechanisms and possible drug targets for diseases in which abnormal kinase activity is linked to onset and progression. Here, we describe a mass spectrometry-based strategy to define the direct substrates of a kinase of interest. The kinase assay-linked phosphoproteomics approach examines putative kinase substrates both in vitro and in vivo to produce a list of highly confident substrates.
authors.affiliation: Purdue University, West Lafayette, IN, United States; Purdue University Institute for Integrative Neuroscience, Purdue University, West Lafayette, IN, United States.
authors.forename: J V
authors.initials: JV
authors.lastname: Arrington
authors.affiliation: Purdue University, West Lafayette, IN, United States.
authors.forename: C-C
authors.initials: CC
authors.lastname: Hsu
authors.affiliation: Purdue University, West Lafayette, IN, United States; Purdue University Institute for Integrative Neuroscience, Purdue University, West Lafayette, IN, United States; Purdue University Center for Cancer Research, Purdue University, West Lafayette, IN, United States. Electronic address: taow@purdue.edu.
authors.forename: W A
authors.initials: WA
authors.lastname: Tao
mesh_terms: D000818:Animals; D057075:Enzyme Assays; D006801:Humans; D010750:Phosphoproteins; D011494:Protein Kinases / Q000737:chemistry*; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000737:chemistry*; D040901:Proteomics; D013379:Substrate Specificity
chemical_list: D010750:Phosphoproteins; D020543:Proteome; D011494:Protein Kinases
keywords: Kinase; Mass spectrometry; Phosphoproteomics; Phosphorylation; Signal transduction