title: Candidate pheromone receptors of codling moth Cydia pomonella respond to pheromones and kairomones.
abstract: Olfaction plays a dominant role in the mate-finding and host selection behaviours of the codling moth (Cydia pomonella), an important pest of apple, pear and walnut orchards worldwide. Antennal transcriptome analysis revealed a number of abundantly expressed genes related to the moth olfactory system, including those encoding the olfactory receptors (ORs) CpomOR1, CpomOR3 and CpomOR6a, which belong to the pheromone receptor (PR) lineage, and the co-receptor (CpomOrco). Using heterologous expression, in both Drosophila olfactory sensory neurones and in human embryonic kidney cells, together with electrophysiological recordings and calcium imaging, we characterize the basic physiological and pharmacological properties of these receptors and demonstrate that they form functional ionotropic receptor channels. Both the homomeric CpomOrco and heteromeric CpomOrco + OR complexes can be activated by the common Orco agonists VUAA1 and VUAA3, as well as inhibited by the common Orco antagonists amiloride derivatives. CpomOR3 responds to the plant volatile compound pear ester ethyl-(E,Z)-2,4-decadienoate, while CpomOR6a responds to the strong pheromone antagonist codlemone acetate (E,E)-8,10-dodecadien-1-yl acetate. These findings represent important breakthroughs in the deorphanization of codling moth pheromone receptors, as well as more broadly into insect ecology and evolution and, consequently, for the development of sustainable pest control strategies based on manipulating chemosensory communication.
authors.affiliation: Fondazione Edmund Mach, Research and Innovation Centre/DASB, Agricultural Entomology, San Michele all'Adige, TN, Italy.|University of Florida, Whitney Laboratory, Center for Smell and Taste, and McKnight Brain Institute, Gainesville, FL, USA.|Università degli Studi di Milano, Department of Food, Nutritional and Environmental Sciences, Milan, Italy.|Università di Cagliari, Dipartimento di Scienze Biomediche, Monserrato, CA, Italy.
authors.forename: Alberto Maria
authors.initials: AM
authors.lastname: Cattaneo
authors.affiliation: Swedish University of Agricultural Sciences, Department of Plant Protection Biology, Chemical Ecology Unit, Alnarp, Sweden.
authors.forename: Francisco
authors.initials: F
authors.lastname: Gonzalez
authors.affiliation: Stockholm University, Department of Zoology, Stockholm, Sweden.
authors.forename: Jonas M
authors.initials: JM
authors.lastname: Bengtsson
authors.affiliation: University of Florida, Whitney Laboratory, Center for Smell and Taste, and McKnight Brain Institute, Gainesville, FL, USA.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Corey
authors.affiliation: INRA, Institute of Ecology and Environmental Sciences (iEES-Paris), Versailles, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Jacquin-Joly
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, iEES-Paris, Paris, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Montagné
authors.affiliation: Fondazione Edmund Mach, Research and Innovation Centre/DASB, Agricultural Entomology, San Michele all'Adige, TN, Italy.
authors.forename: Umberto
authors.initials: U
authors.lastname: Salvagnin
authors.affiliation: Swedish University of Agricultural Sciences, Department of Plant Protection Biology, Chemical Ecology Unit, Alnarp, Sweden.|Max Planck Institute for Chemical Ecology, Department of Evolutionary Neuroethology, Jena, Germany.
authors.forename: William B
authors.initials: WB
authors.lastname: Walker
authors.affiliation: Swedish University of Agricultural Sciences, Department of Plant Protection Biology, Chemical Ecology Unit, Alnarp, Sweden.
authors.forename: Peter
authors.initials: P
authors.lastname: Witzgall
authors.affiliation: Fondazione Edmund Mach, Research and Innovation Centre/DASB, Agricultural Entomology, San Michele all'Adige, TN, Italy.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Anfora
authors.affiliation: University of Florida, Whitney Laboratory, Center for Smell and Taste, and McKnight Brain Institute, Gainesville, FL, USA.
authors.forename: Yuriy V
authors.initials: YV
authors.lastname: Bobkov
mesh_terms: D000818:Animals; D002460:Cell Line; D003651:Decanoates / Q000494:pharmacology*; D007851:Dodecanol / Q000031:analogs & derivatives; D004330:Drosophila / Q000187:drug effects; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D019476:Insect Proteins / Q000819:agonists*; D008297:Male; D009036:Moths / Q000378:metabolism*; D018034:Olfactory Receptor Neurons / Q000187:drug effects / Q000378:metabolism; D010675:Pheromones / Q000494:pharmacology*; D043563:Receptors, Pheromone / Q000819:agonists* / Q000378:metabolism
chemical_list: C499391:(4E,10E)-dodecadien-1-yl acetate; D003651:Decanoates; D019476:Insect Proteins; D010675:Pheromones; D043563:Receptors, Pheromone; C438223:ethyl-2,4-decadienoate; D007851:Dodecanol