title: Systematic evaluation of serum and plasma collection on the endogenous metabolome.
abstract: OBJECTIVE
  In metabolomics research, the use of different blood collection methods may influence endogenous metabolites.
  
  
  METHODS
  Ultra HPLC coupled with MS/MS was applied together with multivariate statistics to investigate metabolomics differences in serum and plasma samples handled by different anticoagulants. A total of 135 known representative metabolites were assessed for comprehensive evaluation of the effects of anticoagulants.
  
  
  RESULTS
  Exogenous factors, including separation gel ingredients from the serum collection tubes and the anticoagulants, affected mass spectrometer detection. Heparin plasma yielded the best detection of different functional groups and is therefore the optimal blood specimen for metabolomics research, followed by potassium oxalate plasma.
authors.affiliation: State Key Laboratory of Bioactive Substance & Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100050, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Bioactive Substance & Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100050, China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Bioactive Substance & Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100050, China.
authors.forename: Jiuming
authors.initials: J
authors.lastname: He
authors.affiliation: State Key Laboratory of Bioactive Substance & Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100050, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Bioactive Substance & Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100050, China.
authors.forename: Ruiping
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Xinjiang Institute of Materia Medica, Urumqi 830004, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Mao
authors.affiliation: State Key Laboratory of Bioactive Substance & Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100050, China.|Minzu University of China, Beijing 100081, P. R. China.
authors.forename: Zeper
authors.initials: Z
authors.lastname: Abliz
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D008297:Male; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D010949:Plasma / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D044967:Serum / Q000737:chemistry*; D013048:Specimen Handling / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
keywords: LC–MS; anticoagulants; metabolomics; multivariate statistical analysis; plasma; serum