title: RhBMP-2 loaded 3D-printed mesoporous silica/calcium phosphate cement porous scaffolds with enhanced vascularization and osteogenesis properties.
abstract: A major limitation in the development of effective scaffolds for bone regeneration has been the limited vascularization of the regenerating tissue. Here, we propose the development of a novel calcium phosphate cement (CPC)-based scaffold combining the properties of mesoporous silica (MS) with recombinant human bone morphogenic protein-2 (rhBMP-2) to facilitate vascularization and osteogenesis. Specifically, the development of a custom MS/CPC paste allowed the three-dimensional (3D) printing of scaffolds with a defined macroporous structure and optimized silicon (Si) ions release profile to promote the ingrowth of vascular tissue at an early stage after implantation in support of tissue viability and osteogenesis. In addition, the scaffold microstructure allowed the prolonged release of rhBMP-2, which in turn significantly stimulated the osteogenesis of human bone marrow stromal cells in vitro and of bone regeneration in vivo as shown in a rabbit femur defect repair model. Thus, the combination MS/CPC/rhBMP-2 scaffolds might provide a solution to issues of tissue necrosis during the regeneration process and therefore might be able to be readily developed into a useful tool for bone repair in the clinic.
authors.affiliation: School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.|Shanghai Key Laboratory of Orthopaedic Implants, Shanghai Ninth People's Hospital Affiliated Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Cuidi
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Orthopaedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 107 West Yanjiang Road, Guangzhou, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Jiang
authors.affiliation: School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.|Shanghai Key Laboratory of Orthopaedic Implants, Shanghai Ninth People's Hospital Affiliated Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Shanghai Key Laboratory of Orthopaedic Implants, Shanghai Ninth People's Hospital Affiliated Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: Shanghai Key Laboratory of Orthopaedic Implants, Shanghai Ninth People's Hospital Affiliated Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Mingzheng
authors.initials: M
authors.lastname: Peng
authors.affiliation: School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.|Shanghai Key Laboratory of Orthopaedic Implants, Shanghai Ninth People's Hospital Affiliated Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Jinwu
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D001843:Bone Cements / Q000494:pharmacology; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage* / Q000235:genetics; D001861:Bone Regeneration / Q000187:drug effects*; D017610:Calcium Compounds / Q000008:administration & dosage / Q000737:chemistry*; D002130:Calcium Phosphates / Q000008:administration & dosage / Q000737:chemistry; D002454:Cell Differentiation / Q000187:drug effects; D005269:Femur / Q000187:drug effects / Q000254:growth & development; D006801:Humans; D010012:Osteogenesis / Q000187:drug effects* / Q000235:genetics; D066330:Printing, Three-Dimensional; D011817:Rabbits; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics; D017640:Silicates / Q000008:administration & dosage / Q000737:chemistry*; D012822:Silicon Dioxide / Q000008:administration & dosage / Q000737:chemistry; D054457:Tissue Scaffolds / Q000737:chemistry; D016212:Transforming Growth Factor beta / Q000008:administration & dosage* / Q000235:genetics
chemical_list: D001843:Bone Cements; D055396:Bone Morphogenetic Protein 2; D017610:Calcium Compounds; D002130:Calcium Phosphates; D011994:Recombinant Proteins; D017640:Silicates; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D012822:Silicon Dioxide; C031293:calcium silicate