title: Environmentally sensitive probes for monitoring protein-membrane interactions at nanomolar concentrations.
abstract: Solvatochromic probes are suitable tools for quantitative characterization of protein-membrane interactions. Based on diverse fluorophores these probes have different fluorescent properties and therefore demonstrate different responses when applied for sensing the interactions of biomolecules. Surprisingly, to the best of our knowledge, no systematic comparison of the sensitivities of solvatochromic dyes for monitoring protein-membrane interactions was described. Hence, a rational choice of an optimal environmentally sensitive probe for such experiments is usually not a straightforward task. In this work we developed a series of thiol-reactive fluorescent probes based on the fluorophores with high sensitivity to their environment and compared them with two widely used DNS and DMN probes. We investigated the responses of these probes to the interaction of probe-labeled presynaptic protein α-synuclein with lipid membranes. We observed that newly synthesized probes based on fluorene and chromone dyes, which combine the strongest brightness and significant changes of fluorescence intensity, demonstrated the highest sensitivity to interaction of α-synuclein with lipid membranes. They are especially beneficial for sensing in scattering media such as solutions of lipid vesicles. We show that the described probes permit quantitative measurements of α-synuclein binding to lipid membranes at low nanomolar concentrations. We developed a detailed protocol for measuring Kdand binding stoichiometry for interaction of soluble peripheral proteins with membranes based on the response of the environmentally sensitive fluorescent probes. We applied this protocol for quantification of the affinity of α-synuclein to anionic membranes and found that it is substantially higher than it was earlier reported.
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo namesti 2, 16610 Prague 6, Czech Republic.
authors.forename: Volodymyr V
authors.initials: VV
authors.lastname: Shvadchak
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo namesti 2, 16610 Prague 6, Czech Republic.
authors.forename: Oleksandr
authors.initials: O
authors.lastname: Kucherak
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo namesti 2, 16610 Prague 6, Czech Republic.
authors.forename: Kseniia
authors.initials: K
authors.lastname: Afitska
authors.affiliation: European Molecular Biology Laboratory (EMBL), Cell Biology & Biophysics Unit, Meyerhofstraße 1, 69117 Heidelberg, Germany.
authors.forename: Dmytro
authors.initials: D
authors.lastname: Dziuba
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo namesti 2, 16610 Prague 6, Czech Republic. Electronic address: yushchenko@uochb.cas.cz.
authors.forename: Dmytro A
authors.initials: DA
authors.lastname: Yushchenko
mesh_terms: D005456:Fluorescent Dyes; D008027:Light; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D012542:Scattering, Radiation; D051844:alpha-Synuclein / Q000737:chemistry* / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D051844:alpha-Synuclein
keywords: Affinity; Binding stoichiometry; Fluorescence; Protein-membrane interaction; Solvatochromic probes; α-synuclein