title: The Anti-Inflammatory and Vasodilating Effects of Three Selected Dietary Organic Sulfur Compounds from Allium Species.
abstract: The anti-inflammatory and vasodilating effects of three selected dietary organic sulfur compounds (OSC), including diallyl disulfide (DADS), dimethyl disulfide (DMDS), and propyl disulfide (PDS), fromAlliumspecies were investigated. In the anti-inflammatory activity assay, the three OSC demonstrated significant inhibition of nitric oxide (NO) and prostaglandin E₂ (PGE₂) production in LPS-induced RAW 264.7 cells. The expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX-2) in activated RAW 264.7 cells was inhibited by the three OSC, indicating that the three OSC prevented the LPS-induced inflammatory response in RAW 264.7 cells. For the vasodilative assay, the three OSC were ineffective in producing NO in SVEC4-10 cells, but they did enhance prostacyclin (PGI₂) production. The expression of COX-2 in SVEC4-10 cells was activated by DADS and DMDS. Pretreatment of SVEC4-10 cells with the three OSC decreased ROS generation in H₂O₂-induced SVEC4-10 cells. In addition, the three OSC significantly inhibited angiotensin-I converting enzyme (ACE). The up-regulation of PGI₂ production and COX-2 expression by DADS and DMDS and the reduction of ROS generation by DADS, DMDS, and PDS in SVEC4-10 cells contributed to the vasodilative effect of the three OSC. Collectively, these findings suggest that DADS, DMDS, and PDS are potential anti-inflammatory and vasodilative mediators.
authors.affiliation: Department of Anesthesiology, Chi-Mei Medical Center, Tainan 71004, Taiwan. chinchen.chu@gmail.com.|Department of Recreation and Health-Care Management, Chi-Mei Medical Center, Tainan 71004, Taiwan. chinchen.chu@gmail.com.
authors.forename: Chin-Chen
authors.initials: CC
authors.lastname: Chu
authors.affiliation: Division of Cardiology, Chi-Mei Medical Center, Tainan 71004, Taiwan. yyl.yehaline@gmail.com.|Center of General Education, Chi-Mei Medical Center, Tainan 71004, Taiwan. yyl.yehaline@gmail.com.
authors.forename: Wen-Shiann
authors.initials: WS
authors.lastname: Wu
authors.affiliation: Department of Anesthesiology, Chi-Mei Medical Center, Tainan 71004, Taiwan. dog.i7007@msa.hinet.net.
authors.forename: Ja-Ping
authors.initials: JP
authors.lastname: Shieh
authors.affiliation: Department of Food Science and Technology, Chia Nan University of Pharmacy and Science, Tainan 71710, Taiwan. chuheuy@mail.cnu.edu.tw.
authors.forename: Heuy-Ling
authors.initials: HL
authors.lastname: Chu
authors.affiliation: Department of Food Science and Technology, Chia Nan University of Pharmacy and Science, Tainan 71710, Taiwan. pulee@mail.cnu.edu.tw.
authors.forename: Chia-Pu
authors.initials: CP
authors.lastname: Lee
authors.affiliation: Department of Food Science and Technology, Chia Nan University of Pharmacy and Science, Tainan 71710, Taiwan. ipdduh@mail.cnu.edu.tw.
authors.forename: Pin-Der
authors.initials: PD
authors.lastname: Duh
keywords: angiotensin-converting enzyme; anti-inflammatory activity; diallyl disulfide; dimethyl disulfide; propyl disulfide; prostacyclin; vasodilation