title: Multicenter Evaluation of the Portrait Staph ID/R Blood Culture Panel for Rapid Identification of Staphylococci and Detection of themecAGene.
abstract: Bloodstream infections are a leading cause of morbidity and mortality in the United States and are associated with increased health care costs. We evaluated the Portrait Staph ID/R blood culture panel (BCP) multiplex PCR assay (Great Basin Scientific, Salt Lake City, UT) for the rapid and simultaneous identification (ID) ofStaphylococcus aureus,Staphylococcus lugdunensis, andStaphylococcusspecies to the genus level and the detection of themecAgene directly from a positive blood culture bottle. A total of 765 Bactec bottles demonstrating Gram-positive cocci in singles or clusters were tested during the prospective trial at 3 clinical sites. The Portrait Staph ID/R BCP results were compared with results from conventional biochemical and cefoxitin disk methods performed at an independent laboratory. Discordant ID andmecAresults were resolved byrpoBgene sequencing andmecAgene sequencing, respectively. A total of 658Staphylococcusspecies isolates (S. aureus, 211 isolates;S. lugdunensis, 3 isolates; andStaphylococcusspp., 444 isolates) were recovered from monomicrobial and 33 polymicrobial blood cultures. After discrepant analysis, the overall ratios of Portrait Staph ID/R BCP positive percent agreement and negative percent agreement were 99.4%/99.9% forStaphylococcusID and 99.7%/99.2% formecAdetection.
authors.affiliation: Indiana University School of Medicine, Indianapolis, Indiana, USA gdenys@iupui.edu.
authors.forename: Gerald A
authors.initials: GA
authors.lastname: Denys
authors.affiliation: Indiana University School of Medicine, Indianapolis, Indiana, USA.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Collazo-Velez
authors.affiliation: University of New Mexico/Tricore Laboratories, Albuquerque, New Mexico, USA.
authors.forename: Stephen
authors.initials: S
authors.lastname: Young
authors.affiliation: University of Utah, Primary Children's Medical Center, Salt Lake City, Utah, USA.
authors.forename: Judy A
authors.initials: JA
authors.lastname: Daly
authors.affiliation: Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA.|ARUP Laboratories, Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA.
authors.forename: Marc Roger
authors.initials: MR
authors.lastname: Couturier
authors.affiliation: Medical College of Wisconsin, Milwaukee, Wisconsin, USA.|ARUP Laboratories, Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA.
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Faron
authors.affiliation: Medical College of Wisconsin, Milwaukee, Wisconsin, USA.|ARUP Laboratories, Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA.
authors.forename: Blake W
authors.identifier: 0000-0002-4111-048X
authors.initials: BW
authors.lastname: Buchan
authors.affiliation: Medical College of Wisconsin, Milwaukee, Wisconsin, USA.|ARUP Laboratories, Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA.
authors.forename: Nathan
authors.initials: N
authors.lastname: Ledeboer
mesh_terms: D000071997:Blood Culture / Q000379:methods*; D005798:Genes, Bacterial; D006801:Humans; D016106:Methicillin Resistance; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D011446:Prospective Studies; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013210:Staphylococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D013997:Time Factors; D014481:United States
keywords: PCR; Staphylococcus; blood culture; genotypic identification; mecA detection; rapid tests