title: Cesium reversibly suppresses HeLa cell proliferation by inhibiting cellular metabolism.
abstract: The aim of the present study was to investigate the influence of Cs+on cultured human cells. We find that HeLa cell growth is suppressed by the addition of 10 mm CsCl into the culture media. In the Cs+-treated cells, the intracellular Cs+and K+concentrations are increased and decreased, respectively. This leads to a decrease in activity of the glycolytic enzyme pyruvate kinase, which uses K+as a cofactor. Cs+-treated cells show an intracellular pH shift towards alkalization. Based on these results, CsCl presumably suppresses HeLa cell proliferation by inducing an intracellular cation imbalance that affects cell metabolism. Our findings may have implications for the use of Cs+in cancer therapy.
authors.affiliation: Department of Cellular and Integrative Physiology, School of Medicine, Fukushima Medical University, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kobayashi
authors.affiliation: Department of Cellular and Integrative Physiology, School of Medicine, Fukushima Medical University, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Kakinouchi
authors.affiliation: Department of Cellular and Integrative Physiology, School of Medicine, Fukushima Medical University, Japan.
authors.forename: Tomoki
authors.initials: T
authors.lastname: Nagae
authors.affiliation: Department of Natural Sciences, School of Medicine, Fukushima Medical University, Japan.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Nagai
authors.affiliation: Department of Natural Sciences, School of Medicine, Fukushima Medical University, Japan.
authors.forename: Kiyohito
authors.initials: K
authors.lastname: Shimura
authors.affiliation: Department of Cellular and Integrative Physiology, School of Medicine, Fukushima Medical University, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Hazama
mesh_terms: D002414:Cations, Monovalent; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002586:Cesium / Q000378:metabolism / Q000494:pharmacology*; D002712:Chlorides / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry; D006019:Glycolysis / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011188:Potassium / Q000378:metabolism; D011770:Pyruvate Kinase / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D002414:Cations, Monovalent; D002712:Chlorides; D003470:Culture Media; D002586:Cesium; D011770:Pyruvate Kinase; C028019:cesium chloride; D011188:Potassium
keywords: cell cycle; cesium; proliferation