title: Gene expression profiling analysis of the effects of low-intensity pulsed ultrasound on induced pluripotent stem cell-derived neural crest stem cells.
abstract: Low-intensity pulsed ultrasound (LIPUS) is a noninvasive technique that has been shown to affect cell proliferation, migration, and differentiation and promote the regeneration of damaged peripheral nerve. Our previous studies had proved that LIPUS can significantly promote the neural differentiation of induced pluripotent stem cell-derived neural crest stem cells (iPSCs-NCSCs) and enhance the repair of rat-transected sciatic nerve. To further explore the underlying mechanisms of LIPUS treatment of iPSCs-NCSCs, this study reported the gene expression profiling analysis of iPSCs-NCSCs before and after LIPUS treatment using the RNA-sequencing (RNA-Seq) method. It was found that expression of 76 genes of iPSCs-NCSCs cultured in a serum-free neural induction medium and expression of 21 genes of iPSCs-NCSCs cultured in a neuronal differentiation medium were significantly changed by LIPUS treatment. The differentially expressed genes are related to angiogenesis, nervous system activity and functions, cell activities, and so on. The RNA-seq results were further verified by a quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR). High correlation was observed between the results obtained from qRT-PCR and RNA-Seq. This study presented new information on the global gene expression patterns of iPSCs-NCSCs after LIPUS treatment and may expand the understanding of the complex molecular mechanism of LIPUS treatment of iPSCs-NCSCs.
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing, People's Republic of China.|Mechanobiology and Regenerative Medicine Laboratory, Bioengineering College, Chongqing University, Chongqing, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Xia
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing, People's Republic of China.|Mechanobiology and Regenerative Medicine Laboratory, Bioengineering College, Chongqing University, Chongqing, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing, People's Republic of China.|Mechanobiology and Regenerative Medicine Laboratory, Bioengineering College, Chongqing University, Chongqing, People's Republic of China.
authors.forename: Zhiling
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing, People's Republic of China.|Mechanobiology and Regenerative Medicine Laboratory, Bioengineering College, Chongqing University, Chongqing, People's Republic of China.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Lv
mesh_terms: D002478:Cells, Cultured; D020869:Gene Expression Profiling; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D009432:Neural Crest / Q000166:cytology / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D000069453:Ultrasonic Waves
keywords: RNA-sequencing; differentially expressed genes; induced pluripotent stem cells; low-intensity pulsed ultrasound; peripheral nerve repair