title: Proteomics-Based Identification of the Molecular Signatures of Liver Tissues from Aged Rats following Eight Weeks of Medium-Intensity Exercise.
abstract: Physical activity has emerged as a powerful intervention that promotes healthy aging by maintaining the functional capacity of critical organ systems. Here, by combining functional and proteomics analyses, we examined how hepatic phenotypes might respond to exercise treatment in aged rats. 16 male aged (20 months old) SD rats were divided into exercise and parallel control groups at random; the exercise group had 8 weeks of treadmill training with medium intensity. Whole protein samples of the liver were extracted from both groups and separated by two-dimensional gel electrophoresis. Alternatively objective protein spots with >2-fold difference in expression were selected for enzymological extraction and MS/MS identification. Results show increased activity of the manganese superoxide dismutase and elevated glutathione levels in the livers of exercise-treated animals, but malondialdehyde contents obviously decreased in the liver of the exercise group. Proteomics-based identification of differentially expressed proteins provided an integrated view of the metabolic adaptations occurring in the liver proteome during exercise, which significantly altered the expression of several proteins involved in key liver metabolic pathways including mitochondrial sulfur, glycolysis, methionine, and protein metabolism. These findings indicate that exercise may be beneficial to aged rats through modulation of hepatic protein expression profiles.
authors.affiliation: School of Physical Education and Health, Zhaoqing University, Zhaoqing 526016, China; Exercise Health and Technology Centre, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Fanghui
authors.identifier: 0000-0002-6612-7608
authors.initials: F
authors.lastname: Li
authors.affiliation: Laboratory of Laser Sports Medicine, South China Normal University, Guangzhou 510006, China.
authors.forename: Tao
authors.identifier: 0000-0002-2690-891X
authors.initials: T
authors.lastname: Li
authors.affiliation: School of Physical Education and Health, Zhaoqing University, Zhaoqing 526016, China.
authors.forename: Yanying
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000375:Aging; D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005978:Glutathione / Q000032:analysis; D008099:Liver / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D009929:Organ Size; D010805:Physical Conditioning, Animal; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013482:Superoxide Dismutase
chemical_list: D020543:Proteome; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione