title: Suppressing role of miR-520a-3p in breast cancer through CCND1 and CD44.
abstract: Recent studies show that many microRNAs (miRNAs) are found to play important roles in breast cancer, however, most of miRNAs are not investigated completely. In the present study, significant down-regulation of miR-520a-3p was found in the breast cancer tissues and cell lines. The restoration of miR-520a-3p expression in breast cancer cells could inhibit cell proliferation, migration and invasion. In addition, miR-520a-3p was able to induce breast cancer cell apoptosis. Luciferase assay was used to confirm that CCND1 and CD44 were the direct target genes of miR-520a-3p. The ectopic expression of miR-520a-3p repressed CCND1 and CD44 expression on post-transcriptional levels in breast cancer cells. This study suggests that miR-520a-3p may act as an optional method for breast cancer therapy.
authors.affiliation: Department of Medical Oncology, The First Affiliated Hospital with Nanjing Medical University Nanjing, P. R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Medical Oncology, The Second Affiliated Hospital with Southeast University Nanjing, P. R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wei
authors.affiliation: Department of Medical Oncology, Mingde Hospital of Nanjing Medical University Nanjing, P. R. China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Mei
authors.affiliation: Department of Medical Oncology, The First Affiliated Hospital with Nanjing Medical University Nanjing, P. R. China.
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Department of Medical Oncology, The First Affiliated Hospital with Nanjing Medical University Nanjing, P. R. China.
authors.forename: Yongfei
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Medical Oncology, The First Affiliated Hospital with Nanjing Medical University Nanjing, P. R. China.
authors.forename: Mingjie
authors.initials: M
authors.lastname: Lu
authors.affiliation: Department of Medical Oncology, The First Affiliated Hospital with Nanjing Medical University Nanjing, P. R. China.
authors.forename: Shidai
authors.initials: S
authors.lastname: Jin
keywords: CCND1; CD44; breast cancer; metastasis; miR-520a-3p; proliferation