title: Effect of moisture equilibration time and medium on contact angles of bacterial spores.
abstract: Contact angle measurement of microorganisms is often described in literature, either to investigate their hydrophobic characteristic or the adhesion behavior of cells. However, in some key aspects the preparation methods differ. Thus, it is difficult to compare results and to choose a procedure for repetition of measurements. The aim of this paper is to point out some critical points during microorganism film preparation that can alter the resulting contact angles. Depending on the moisturizing medium and equilibration time, contact angles differ significantly.
authors.affiliation: Chair of Food and Bioprocess Engineering, Technical University of Munich, Weihenstephaner Berg 1, Freising, DE, Germany; ZIEL Institute for Food & Health. Electronic address: elisabeth.eschlbeck@tum.de.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Eschlbeck
authors.affiliation: Chair of Food and Bioprocess Engineering, Technical University of Munich, Weihenstephaner Berg 1, Freising, DE, Germany; ZIEL Institute for Food & Health.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Kulozik
mesh_terms: D001407:Bacillus / Q000166:cytology / Q000187:drug effects / Q000235:genetics; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003470:Culture Media / Q000494:pharmacology*; D056506:Geobacillus / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D005990:Glycerol / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D008855:Microscopy, Electron, Scanning; D013171:Spores, Bacterial / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology; D013499:Surface Properties / Q000187:drug effects; D013997:Time Factors; D014867:Water / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D014867:Water; D005990:Glycerol
keywords: Adhesion; Bacillus; Bacterial spores; Contact angle; Hydrophobicity