title: Protective effects of recombinant human cytoglobin against chronic alcohol-induced liver disease in vivo and in vitro.
abstract: Alcoholic liver disease (ALD) is an important worldwide public health issue with no satisfying treatment available since now. Here we explore the effects of recombinant human cytoglobin (rhCygb) on chronic alcohol-induced liver injury and the underlying mechanisms. In vivo studies showed that rhCygb was able to ameliorate alcohol-induced liver injury, significantly reversed increased serum index (ALT, AST, TG, TC and LDL-C) and decreased serum HDL-C. Histopathology observation of the liver of rats treated with rhCygb confirmed the biochemical data. Furthermore, rhCygb significantly inhibited Kupffer cells (KCs) proliferation and TNF-α expression in LPS-induced KCs. rhCygb also inhibited LPS-induced NADPH oxidase activity and ROS, NO and O2•-generation. These results collectively indicate that rhCygb exert the protective effect on chronic alcohol-induced liver injury through suppression of KC activation and oxidative stress. In view of its anti-oxidative stress and anti-inflammatory features, rhCygb might be a promising candidate for development as a therapeutic agent against ALD.
authors.affiliation: School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong Province 510515, P.R. China.|Department of Hematology, Nanxishan Hospital of Guangxi Zhuang Autonomous Region, Guilin, Guangxi Zhuang Autonomous Region 541002, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wen
authors.affiliation: Department of Clinical Laboratory, Nanxishan Hospital of Guangxi Zhuang Autonomous Region, Guilin, Guangxi Zhuang Autonomous Region 541002, P.R. China.
authors.forename: Yongbin
authors.initials: Y
authors.lastname: Wu
authors.affiliation: School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong Province 510515, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong Province 510515, P.R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.affiliation: School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong Province 510515, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Hematology, Nanxishan Hospital of Guangxi Zhuang Autonomous Region, Guilin, Guangxi Zhuang Autonomous Region 541002, P.R. China.
authors.forename: Shiwei
authors.initials: S
authors.lastname: Zhu
authors.affiliation: School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong Province 510515, P.R. China.
authors.forename: Wenqi
authors.initials: W
authors.lastname: Dong
mesh_terms: D000818:Animals; D015415:Biomarkers; D049109:Cell Proliferation / Q000187:drug effects; D000078842:Cytoglobin; D004195:Disease Models, Animal; D005914:Globins / Q000494:pharmacology*; D006801:Humans; D007728:Kupffer Cells / Q000187:drug effects / Q000378:metabolism; D008070:Lipopolysaccharides / Q000009:adverse effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008108:Liver Diseases, Alcoholic / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology*; D008111:Liver Function Tests; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D015415:Biomarkers; C000627265:CYGB protein, human; C000627268:Cygb protein, rat; D000078842:Cytoglobin; D008070:Lipopolysaccharides; D020011:Protective Agents; D017382:Reactive Oxygen Species; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D005914:Globins