title: Evaluation of the biofilm forming ability and its associated genes in Staphylococcus species isolates from bovine mastitis in Argentinean dairy farms.
abstract: Staphylococcus aureus and coagulase-negative staphylococci (CNS) are important causes of intramammary infection in dairy cattle, and their ability to produce biofilm is considered an important virulence property in the pathogenesis of mastitis. However, the published date on mechanisms and factors involved in infection persistence in the mammary gland remains unclear. The aim of this study was to investigate whether the main Staphylococcus species involved in bovine intramammary infections possess specific characteristics that promote colonization of the udder. We evaluated the biofilm-forming ability and distribution of adhesion- and biofilm-associated genes of Staphylococcus spp. isolated from bovine mastitis infected animals in Argentinean dairy farms. For this purpose, the phenotypic biofilm formation ability of 209 Staphylococcus spp. from bovine mastitis was investigated. All isolates produced biofilm in vitro, being 35,0% and 45,0% of the 127 S. aureus or 51,0% and 29,0% of the 82 CNS strong and moderate biofilm producers respectively. All S. aureus samples were PCR-positive for icaA, icaD, clfA, clfB and fnbpA genes, 76.3% were positive for fnbpB gene and 11.0% were positive for bap gene. In CNS isolates, the positive rates for icaA and icaD were 73.2%, while for clfA, clfB, fnbpA fnbpB and bap genes the percentage were lower. The results demonstrate that in Staphylococcus spp. biofilm formation, the polysaccharide and the adhesion- and biofilm-associated genes are of overall importance on bovine mastitis in Argentina. Therefore, future works should focus on these pathogenic specific factors for the development of more effective therapies of control, being essential to consider the ability of isolates to produce biofilm.
authors.affiliation: Instituto Académico Pedagógico de Ciencias Básicas y Aplicadas (IAPCByA), Universidad Nacional de Villa María (UNVM), Villa María, Argentina; Centro de Investigaciones y Transferencia de Villa María (CIT-VM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Villa María (UNVM), Villa María, Argentina.
authors.forename: Verónica
authors.initials: V
authors.lastname: Felipe
authors.affiliation: Instituto Académico Pedagógico de Ciencias Básicas y Aplicadas (IAPCByA), Universidad Nacional de Villa María (UNVM), Villa María, Argentina.
authors.forename: Carolina A
authors.initials: CA
authors.lastname: Morgante
authors.affiliation: Instituto Académico Pedagógico de Ciencias Básicas y Aplicadas (IAPCByA), Universidad Nacional de Villa María (UNVM), Villa María, Argentina.
authors.forename: Paola S
authors.initials: PS
authors.lastname: Somale
authors.affiliation: Instituto Académico Pedagógico de Ciencias Básicas y Aplicadas (IAPCByA), Universidad Nacional de Villa María (UNVM), Villa María, Argentina.
authors.forename: Florencia
authors.initials: F
authors.lastname: Varroni
authors.affiliation: Instituto Académico Pedagógico de Ciencias Básicas y Aplicadas (IAPCByA), Universidad Nacional de Villa María (UNVM), Villa María, Argentina.
authors.forename: María L
authors.initials: ML
authors.lastname: Zingaretti
authors.affiliation: Instituto Académico Pedagógico de Ciencias Básicas y Aplicadas (IAPCByA), Universidad Nacional de Villa María (UNVM), Villa María, Argentina.
authors.forename: Romina A
authors.initials: RA
authors.lastname: Bachetti
authors.affiliation: Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI)-Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
authors.forename: Silvia G
authors.initials: SG
authors.lastname: Correa
authors.affiliation: Instituto Académico Pedagógico de Ciencias Básicas y Aplicadas (IAPCByA), Universidad Nacional de Villa María (UNVM), Villa María, Argentina; Centro de Investigaciones y Transferencia de Villa María (CIT-VM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Villa María (UNVM), Villa María, Argentina. Electronic address: cporporatto@unvm.edu.ar.
authors.forename: Carina
authors.initials: C
authors.lastname: Porporatto
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics; D000818:Animals; D001118:Argentina; D018441:Biofilms / Q000254:growth & development*; D002417:Cattle; D000072480:Farms; D005798:Genes, Bacterial; D008414:Mastitis, Bovine / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology*
chemical_list: D018829:Adhesins, Bacterial
keywords: Biofilm; Dairy cow; Mastitis; Staphylococcus