title: In vitro assessment on effect of duodenal contents on the lead (Pb2+) binding capacity of two probiotic bacterial strains.
abstract: In vitro Lead (Pb2+) binding capacity of two probiotic bacteria strains, namely Bifidobacterium longumBB79 and Lactobacillus pentosusITA23, was assessed following incubation with the intestinal contents (IC) of laying hens. Results of this study demonstrated that IC treatment significantly enhanced (P<0.01) Pb2+binding capacity of both bacterial strains. Fourier transform infrared analysis indicated that several functional groups (O-H or N-H, C-H, C˭O, C-O, and C-O-C) on the bacteria cell wall involved in metal ion binding were altered after IC incubation, and new groups appeared between the 3700cm-1and 4000cm-1bands. Transmission electron microscopy demonstrated that after incubation with IC, unidentified IC components created new binding sites on the bacterial cell surface. These particles also changed the mechanism of Pb2+binding of the two strains from intracellular accumulation to extracellular adsorption.
authors.affiliation: College of Animal Science, South China Agriculture University, Guangzhou 510642, China.
authors.forename: Sicheng
authors.initials: S
authors.lastname: Xing
authors.affiliation: College of Animal Science, South China Agriculture University, Guangzhou 510642, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Song
authors.affiliation: Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, Serdang 43400, Malaysia.
authors.forename: Juan Boo
authors.initials: JB
authors.lastname: Liang
authors.affiliation: Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, Serdang 43400, Malaysia; Agriculture Biotechnology Research Institute of Iran (ABRII), East and North‑East Branch, P.O.B. 91735 844, Mashhad, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Faseleh Jahromi
authors.affiliation: Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, Serdang 43400, Malaysia; Agriculture Biotechnology Research Institute of Iran (ABRII), East and North‑East Branch, P.O.B. 91735 844, Mashhad, Iran.
authors.forename: Parisa
authors.initials: P
authors.lastname: Shokryazda
authors.affiliation: College of Animal Science, South China Agriculture University, Guangzhou 510642, China.
authors.forename: Jiandui
authors.initials: J
authors.lastname: Mi
authors.affiliation: Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
authors.forename: Cui
authors.initials: C
authors.lastname: Zhu
authors.affiliation: College of Food Science, South China Agriculture University, Guangzhou 510642, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Animal Science, South China Agriculture University, Guangzhou 510642, China. Electronic address: xdliao@scau.edu.cn.
authors.forename: Xindi
authors.initials: X
authors.lastname: Liao
mesh_terms: D000818:Animals; D001644:Bifidobacterium / Q000378:metabolism*; D002645:Chickens; D004386:Duodenum; D007408:Intestinal Absorption / Q000502:physiology; D007778:Lactobacillus / Q000378:metabolism*; D007854:Lead / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D019936:Probiotics / Q000378:metabolism
chemical_list: D007854:Lead
keywords: Binding capacity; Functional group; Intestinal contents; Pb(2+); Probiotic bacteria