title: Can environmental pollution by metals change genetic diversity? Ucides cordatus (Linnaeus, 1763) as a study case in Southeastern Brazilian mangroves.
abstract: Industrial areas on estuarine systems are commonly affected by heavy metals, affecting all local biota. Random Amplified Polymorphic DNA (RAPD) was used to evaluate genetic diversity of Ucides cordatus at mangroves in southeastern Brazil (Juréia, J; São Vicente, SV; and Cubatão, C), with distinct pollution levels by metals. The genetic diversity of this species was compared with concentrations of metals (Cd, Pb, Cu, Cr and Hg) in the environment. A pollution gradient was confirmed (SV>C>J), with low levels detected in water, except for mercury in SV. All metals in the sediment samples were below Threshold Effect Level (TEL), without an apparent biological risk to the biota. Genetic distance was very similar between J and C, with SV occurring as an out-group. RAPD was a powerful tool to investigate the effect of metal pollution on genetic diversity of this mangrove crab, and to evaluate the conservation status of the mangrove ecosystem.
authors.affiliation: Instituto Butantan - Laboratório de Ecologia e Evolução, Av. Dr. Vital Brasil, 1500, 05503-900 São Paulo (SP), Brazil.
authors.forename: Karina Rodrigues da Silva
authors.initials: KR
authors.lastname: Banci
authors.affiliation: UNESP - Univ Estadual Paulista, Instituto de Biociências (IB), Campus do Litoral Paulista (CLP) - Pç. Infante Dom Henrique, s/n° - Parque Bitaru, 11330-900 São Vicente (SP), Brazil.
authors.forename: Gustavo Maruyama
authors.initials: GM
authors.lastname: Mori
authors.affiliation: UNESP - Univ Estadual Paulista, Instituto de Biociências (IB), Campus do Litoral Paulista (CLP) - Pç. Infante Dom Henrique, s/n° - Parque Bitaru, 11330-900 São Vicente (SP), Brazil.
authors.forename: Marcos Antonio de
authors.initials: MA
authors.lastname: Oliveira
authors.affiliation: Programa de Genética e Biologia Molecular (UNICAMP) - Campinas (SP), Brazil.
authors.forename: Fernanda Laroza
authors.initials: FL
authors.lastname: Paganelli
authors.affiliation: Programa de Biotecnologia (USP) - São Paulo (SP), Brazil.
authors.forename: Mariana Rangel
authors.initials: MR
authors.lastname: Pereira
authors.affiliation: UNESP - Univ Estadual Paulista, Instituto de Biociências (IB), Campus do Litoral Paulista (CLP) - Pç. Infante Dom Henrique, s/n° - Parque Bitaru, 11330-900 São Vicente (SP), Brazil; Grupo de Pesquisa em Biologia de Crustáceos (CRUSTA), Brazil. Electronic address: pinheiro@clp.unesp.br.
authors.forename: Marcelo Antonio Amaro
authors.initials: MA
authors.lastname: Pinheiro
mesh_terms: D000818:Animals; D003386:Brachyura / Q000235:genetics*; D003247:Conservation of Natural Resources; D017753:Ecosystem; D004784:Environmental Monitoring; D014644:Genetic Variation; D019216:Metals, Heavy; D019105:Random Amplified Polymorphic DNA Technique; D043982:Rhizophoraceae; D014874:Water Pollutants, Chemical
chemical_list: D019216:Metals, Heavy; D014874:Water Pollutants, Chemical
keywords: Biomonitoring; Conservation; Crab; Estuary; Metals; Pollution