title: Single-cell RNA sequencing highlights transcription activity of autophagy-related genes during hematopoietic stem cell formation in mouse embryos.
abstract: Accumulating evidence has demonstrated that macroautophagy/autophagy plays an essential role in self-renewal and differentiation in embryonic hematopoiesis. Here, according to the RNA sequencing data sets of 5 population cells related to hematopoietic stem cell (HSC) formation during mouse embryogenesis (endothelial cells, PTPRC/CD45-and PTPRC/CD45+pre-HSCs in the E11 aorta-gonad-mesonephros (AGM) region, mature HSCs in E12 and E14 fetal liver), we explored the dynamic expression of mouse autophagy-related genes in this course at the single-cell level. Our results revealed that the transcription activity of autophagy-related genes had a substantial increase when endothelial cells (ECs) specified into pre-HSCs, and the upregulation of autophagy-essential genes correlated with reduced NOTCH signaling in pre-HSCs, suggesting the autophagy activity may be greatly enhanced during pre-HSC specification from endothelial precursors. In summary, our results presented strong evidence that autophagy plays a critical role in HSC emergence during mouse midgestation.
authors.affiliation: a College of Bioinformatics Science and Technology , Harbin Medical University , Harbin , China.
authors.forename: Yongfei
authors.initials: Y
authors.lastname: Hu
authors.affiliation: a College of Bioinformatics Science and Technology , Harbin Medical University , Harbin , China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: a College of Bioinformatics Science and Technology , Harbin Medical University , Harbin , China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yi
authors.affiliation: a College of Bioinformatics Science and Technology , Harbin Medical University , Harbin , China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Wang
authors.affiliation: b 307-Ivy Translational Medicine Center , Laboratory of Oncology, Affiliated Hospital, Academy of Military Medical Sciences , Beijing , China.
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.affiliation: c Department of Biochemistry and Molecular Biology , School of Basic Sciences and Institute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences , Beijing , China.
authors.forename: Jia
authors.initials: J
authors.lastname: Yu
authors.affiliation: a College of Bioinformatics Science and Technology , Harbin Medical University , Harbin , China.|d Training Center for Students Innovation and Entrepreneurship Education, Harbin Medical University , Harbin , China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
mesh_terms: D000818:Animals; D001343:Autophagy / Q000235:genetics*; D004622:Embryo, Mammalian / Q000166:cytology*; D018507:Gene Expression Regulation, Developmental; D020043:Genes, Essential; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D051379:Mice; D000066450:Mouse Embryonic Stem Cells / Q000378:metabolism; D051880:Receptors, Notch / Q000378:metabolism; D017423:Sequence Analysis, RNA / Q000379:methods*; D015398:Signal Transduction / Q000235:genetics; D059010:Single-Cell Analysis / Q000379:methods*; D014158:Transcription, Genetic; D015854:Up-Regulation / Q000235:genetics
chemical_list: D051880:Receptors, Notch
keywords: autophagy; hematopoiesis; hematopoietic stem cell; mouse embryos; single cell