title: Solid support resins and affinity purification mass spectrometry.
abstract: Co-affinity purification-mass spectrometry (CoAP-MS) is a primary technology for elucidating the protein-protein interactions that form the basis of all biological processes. A critical component of CoAP-MS is the affinity purification (AP) of the bait protein, usually by immobilization of an antibody to a solid-phase resin. This Minireview discusses common resins, reagents, tagging methods, and their consideration for successful AP of tagged proteins. We discuss our experiences with different solid supports, their impact in AP experiments, and propose areas where chemistry can advance this important technology.
authors.affiliation: Department of Biology and Biochemistry, The University of Houston, 4800 Calhoun Road, SERC 4022, Houston, TX 77204, USA. sbark@uh.edu.
authors.forename: Spencer
authors.initials: S
authors.lastname: Havis
authors.affiliation: Department of Biology and Biochemistry, The University of Houston, 4800 Calhoun Road, SERC 4022, Houston, TX 77204, USA. sbark@uh.edu.
authors.forename: Wilna J
authors.initials: WJ
authors.lastname: Moree
authors.affiliation: Department of Biology and Biochemistry, The University of Houston, 4800 Calhoun Road, SERC 4022, Houston, TX 77204, USA. sbark@uh.edu.
authors.forename: Sujina
authors.initials: S
authors.lastname: Mali
authors.affiliation: Department of Biology and Biochemistry, The University of Houston, 4800 Calhoun Road, SERC 4022, Houston, TX 77204, USA. sbark@uh.edu.
authors.forename: Steven J
authors.identifier: http://orcid.org/0000-0001-6608-6132
authors.initials: SJ
authors.lastname: Bark
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D025941:Protein Interaction Mapping / Q000379:methods