title: Distribution of some pectic and arabinogalactan protein epitopes during Solanum lycopersicum (L.) adventitious root development.
abstract: The adventitious roots (AR) of plants share the same function as primary and lateral roots (LR), although their development is mainly an adaptive reaction to stress conditions. Regeneration of grafted plants is often accompanied by AR formation thus making the grafting technique a good model for studying AR initiation and development and their means of emergence. Pectins and arabinogalactan proteins (AGP) are helpful markers of particular cellular events, such as programmed cell death (PCD), elongation, proliferation or other differentiation events that accompany AR development. However, little is known about the distribution of pectins and AGPs during AR ontogeny, either in the primordium or stem tissues from which AR arise or their correspondence with these events during LR formation.
  
  
  
  AR were developed from different stem tissues such as parenchyma, xylem rays and the cambium, depending on the stem age and treatment (grafting versus cutting) of the parental tissue. Immunochemical analysis of the presence of pectic (LM8, LM19, LM20) and AGP (JIM8, JIM13, JIM16) epitopes in AR and AR-associated tissues showed differential, tissue-specific distributions of these epitopes. Two pectic epitopes (LM19, LM20) were developmentally regulated and the occurrence of the LM8 xylogalacturonan epitope in the root cap of the AR differed from other species described so far. AGP epitopes were abundantly present in the cytoplasmic compartments (mainly the tonoplast) and were correlated with the degree of cell vacuolisation. JIM8 and JIM13 epitopes were detected in the more advanced stages of primordium development, whereas the JIM16 epitope was present from the earliest division events of the initial AR cells. The comparison between AR and LR showed quantitative (AGP,) and qualitative (pectins) differences.
  
  
  
  The chemical compositions of adventitious and lateral root cells show differences that correlate with the different origins of these cells. In AR, developmental changes in the distribution of pectins and AGP suggest the turnover of wall compounds. Our data extend the knowledge about the distribution of pectin and AGP during non-embryogenic root development in a species that is important from an agronomic point of view.
authors.affiliation: Department of Cell Biology, Faculty of Biology and Environmental Protection, University of Silesia, 28 Jagiellońska St, 40-032, Katowice, Poland. ksala@us.edu.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Sala
authors.affiliation: Department of Organic Chemistry, Institute of Chemistry, University of Silesia, 9 Szkolna St, 40-006, Katowice, Poland.|Silesian Center for Education and Interdisciplinary Research, University of Silesia, 75 Pułku Piechoty 1A St, 41-500, Chorzów, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Malarz
authors.affiliation: School of Biological Sciences, University of Bristol, 24 Tyndall Avenue, Bristol, BS8 1TQ, UK.
authors.forename: Peter W
authors.initials: PW
authors.lastname: Barlow
authors.affiliation: Department of Cell Biology, Faculty of Biology and Environmental Protection, University of Silesia, 28 Jagiellońska St, 40-032, Katowice, Poland.
authors.forename: Ewa U
authors.initials: EU
authors.lastname: Kurczyńska
mesh_terms: D000939:Epitopes / Q000276:immunology / Q000378:metabolism; D007150:Immunohistochemistry; D018551:Solanum lycopersicum / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism*; D009088:Mucoproteins / Q000276:immunology / Q000378:metabolism*; D010368:Pectins / Q000378:metabolism; D010940:Plant Proteins / Q000276:immunology / Q000378:metabolism; D018517:Plant Roots / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism*
chemical_list: D000939:Epitopes; D009088:Mucoproteins; D010940:Plant Proteins; C006619:arabinogalactan proteins; D010368:Pectins
keywords: Arabinogalactan proteins; Autografting; Cell differentiation; Cell wall; Immunohistochemistry; Lateral roots; Pectins; Tomatoes