title: Ror2 signaling regulates Golgi structure and transport through IFT20 for tumor invasiveness.
abstract: Signaling through the Ror2 receptor tyrosine kinase promotes invadopodia formation for tumor invasion. Here, we identify intraflagellar transport 20 (IFT20) as a new target of this signaling in tumors that lack primary cilia, and find that IFT20 mediates the ability of Ror2 signaling to induce the invasiveness of these tumors. We also find that IFT20 regulates the nucleation of Golgi-derived microtubules by affecting the GM130-AKAP450 complex, which promotes Golgi ribbon formation in achieving polarized secretion for cell migration and invasion. Furthermore, IFT20 promotes the efficiency of transport through the Golgi complex. These findings shed new insights into how Ror2 signaling promotes tumor invasiveness, and also advance the understanding of how Golgi structure and transport can be regulated.
authors.affiliation: Division of Cell Physiology, Department of Physiology and Cell Biology, Kobe University, Graduate School of Medicine, Kobe, 650-0017, Japan. nishita@med.kobe-u.ac.jp.
authors.forename: Michiru
authors.initials: M
authors.lastname: Nishita
authors.affiliation: Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, and Department of Medicine, Harvard Medical School, Boston, MA, 02115, USA.
authors.forename: Seung-Yeol
authors.initials: SY
authors.lastname: Park
authors.affiliation: Division of Cell Physiology, Department of Physiology and Cell Biology, Kobe University, Graduate School of Medicine, Kobe, 650-0017, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Nishio
authors.affiliation: Division of Cell Physiology, Department of Physiology and Cell Biology, Kobe University, Graduate School of Medicine, Kobe, 650-0017, Japan.
authors.forename: Koki
authors.initials: K
authors.lastname: Kamizaki
authors.affiliation: Division of Cell Physiology, Department of Physiology and Cell Biology, Kobe University, Graduate School of Medicine, Kobe, 650-0017, Japan.
authors.forename: ZhiChao
authors.initials: Z
authors.lastname: Wang
authors.affiliation: RIKEN Brain Science Institute, Wako, 351-0198, Japan.
authors.forename: Kota
authors.initials: K
authors.lastname: Tamada
authors.affiliation: RIKEN Brain Science Institute, Wako, 351-0198, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Takumi
authors.affiliation: Department of Developmental Biology, Faculty of Medicine, Shimane University, Izumo, 690-8504, Japan.
authors.forename: Ryuju
authors.initials: R
authors.lastname: Hashimoto
authors.affiliation: Department of Developmental Biology, Faculty of Medicine, Shimane University, Izumo, 690-8504, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Otani
authors.affiliation: Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, 01605, USA.
authors.forename: Gregory J
authors.identifier: 0000-0002-6285-8796
authors.initials: GJ
authors.lastname: Pazour
authors.affiliation: Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, and Department of Medicine, Harvard Medical School, Boston, MA, 02115, USA.
authors.forename: Victor W
authors.initials: VW
authors.lastname: Hsu
authors.affiliation: Division of Cell Physiology, Department of Physiology and Cell Biology, Kobe University, Graduate School of Medicine, Kobe, 650-0017, Japan. minami@kobe-u.ac.jp.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Minami
mesh_terms: D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D006056:Golgi Apparatus / Q000378:metabolism*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000648:ultrastructure; D008870:Microtubules / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000473:pathology*; D000069261:Podosomes / Q000473:pathology; D034741:RNA, Small Interfering / Q000235:genetics; D057050:Receptor Tyrosine Kinase-like Orphan Receptors / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D002352:Carrier Proteins; C487991:IFT20 protein, human; D034741:RNA, Small Interfering; D057050:Receptor Tyrosine Kinase-like Orphan Receptors