title: Progesterone-Induced miR-152 Inhibits the Proliferation of Endometrial Epithelial Cells by Downregulating WNT-1.
abstract: Progesterone (P4) is an important ovarian hormone that inhibits estrogen-dependent proliferation of endometrial epithelial cells (EECs). miR-152 has been reported to be a cell cycle regulator. In this study, we first demonstrated that P4 induced the expression of miR-152 in ovariectomized mice and Ishikawa cell. miR-152 was detected in the human endometrial cell lines that were stably transfected with P4 receptor. Results showed that P4 induced its expression through its receptor B subtype. Then, using the specific miRNA mimic and inhibitor, we proved that miR-152 impeded G1/S transition in the cell cycle of EECs and inhibited cellular proliferation via downregulating WNT-1 in mice and human endometrial cancer cell lines (Ishikawa, HEC-1-b, and KLE). miR-152 induced by P4 is an important inhibitor for the proliferation of EECs. miR-152 may be an important tumor suppressor microRNA in endometrial cancer.
authors.affiliation: 1 Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Nie
authors.affiliation: 1 Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, People's Republic of China.
authors.forename: You-Bo
authors.initials: YB
authors.lastname: Zhao
authors.affiliation: 1 Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Jun-Li
authors.initials: JL
authors.lastname: Pan
authors.affiliation: 1 Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Lei
authors.affiliation: 1 Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: 1 Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Long
authors.affiliation: 1 Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Jin-Hu
authors.initials: JH
authors.lastname: Zhang
authors.affiliation: 2 Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Hu
authors.affiliation: 1 Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Ming-Qian
authors.initials: MQ
authors.lastname: Xu
authors.affiliation: 1 Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Dong-Zhi
authors.initials: DZ
authors.lastname: Yuan
authors.affiliation: 1 Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Yue
mesh_terms: D000818:Animals; D002452:Cell Count; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D015536:Down-Regulation / Q000187:drug effects*; D004717:Endometrium / Q000187:drug effects* / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D051155:Wnt1 Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: C550857:MIRN152 microRNA, mouse; D035683:MicroRNAs; D051155:Wnt1 Protein; D011374:Progesterone; D004958:Estradiol
keywords: cellular proliferation; endometrial cancer cells; endometrial epithelial cells; miR-152; progesterone