title: Treponema ruminis sp. nov., a spirochaete isolated from the bovine rumen.
abstract: A novel bacterium, strain Ru1T, was encountered during a survey of spirochaetes living in the gastrointestinal tract of ruminants. Comparative analysis of 16S rRNA gene sequence data indicated that strain Ru1T clustered within the genus Treponemabut shared at most 86.1 % sequence similarity with other recognised species of the genus Treponema. Further phylogenetic analysis based on partial recombinase A (recA) gene sequence comparisons, together with phenotypic characterization, also demonstrated the divergence of strain Ru1T from other recognised species of the genus Treponema. Microscopically, strain Ru1T appeared as a very small, highly motile, helical spirochaete with eight periplasmic flagella in a 4 : 8 : 4 arrangement. It exhibited C8 esterase lipase, leucine arylamidase, β-galactosidase and β-glucosidase activity. A distinctive, serum-independent growth pattern was also observed, characterized by colonies with an absence of the local haemolysis that is typical of many pathogenic treponemes. On the basis of these data, strain Ru1T is considered to represent a novel species of the genus Treponema, for which the name Treponema ruminis sp. nov. is proposed. The type strain is Ru1T (=DSM 103462T=NCTC 13847T).
authors.affiliation: Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool, Liverpool Science Park Innovation Centre 2, 146 Brownlow Hill, Liverpool, UK.
authors.forename: Kerry
authors.initials: K
authors.lastname: Newbrook
authors.affiliation: Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool, Liverpool Science Park Innovation Centre 2, 146 Brownlow Hill, Liverpool, UK.
authors.forename: Gareth J
authors.initials: GJ
authors.lastname: Staton
authors.affiliation: Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool, Liverpool Science Park Innovation Centre 2, 146 Brownlow Hill, Liverpool, UK.
authors.forename: Simon R
authors.initials: SR
authors.lastname: Clegg
authors.affiliation: School of Environment and Life Sciences, University of Salford, Greater Manchester, UK.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Birtles
authors.affiliation: Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool, Liverpool Science Park Innovation Centre 2, 146 Brownlow Hill, Liverpool, UK.
authors.forename: Stuart D
authors.initials: SD
authors.lastname: Carter
authors.affiliation: Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool, Liverpool Science Park Innovation Centre 2, 146 Brownlow Hill, Liverpool, UK.
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Evans
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002417:Cattle / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D008297:Male; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012417:Rumen / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014208:Treponema / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006113:United Kingdom
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S