title: Serum microRNAs are non-invasive biomarkers for the presence and progression of subarachnoid haemorrhage.
abstract: miRNAs are important regulators of translation and have been associated with the pathogenesis of a number of cardiovascular diseases including stroke and may be possible prognostic biomarkers. The purpose of the present study was to determine the expression levels of miRNAs in the sera of subarachnoid haemorrhage (SAH) patients and to evaluate their relationships with the severity and clinical outcome of SAH. Serum samples on day 3 after the onset of SAH were subjected to microarray analysis with Exqion miRCURYTMLNA array and quantitative PCR analysis. Serum samples from SAH patients (n=60) and healthy controls (n=10) were subjected to quantitative PCR analysis. The severities and clinical outcomes of the SAH patients were evaluated with the WFNS grade and the Modified Rankin Scale (mRS). Three miRNAs,miR-502-5p,miR-1297andmiR-4320were significantly up-regulated in the sera of SAH patients when compared with the healthy controls. The serummiR-502-5pandmiR-1297levels were significantly higher in the patients with severe SAH and a poor outcome than in those with mild SAH and a good outcome (P<0.05). The areas under the receiver operating characteristic (ROC) curves (AUCs) ofmiR-502-5p,miR-1297andmiR-4320to distinguish the SAH patients from the healthy controls were 0.958 (P<0.001), 0.950 (P<0.001) and 0.843 (P<0.001) respectively. Taken together, these results indicate thatmiR-502-5pandmiR-1297are potentially valuable indicators of the diagnosis, severity and prognosis of SAH, andmiR-4320was a potentially valuable indicator of the diagnosis of SAH.
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Wannan Medical College, Wuhu City, Anhui 241001, China.
authors.forename: Nian-Sheng
authors.initials: NS
authors.lastname: Lai
authors.affiliation: Department of Neurosurgery, Xinxiang Central Hospital , Xinxiang City, Henan 453000, China.
authors.forename: Jia-Qi
authors.initials: JQ
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Wannan Medical College, Wuhu City, Anhui 241001, China.
authors.forename: Fei-Yun
authors.initials: FY
authors.lastname: Qin
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Wannan Medical College, Wuhu City, Anhui 241001, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Sheng
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Wannan Medical College, Wuhu City, Anhui 241001, China.
authors.forename: Xing-Gen
authors.initials: XG
authors.lastname: Fang
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Wannan Medical College, Wuhu City, Anhui 241001, China zhenbaoli123@126.com.
authors.forename: Zhen-Bao
authors.initials: ZB
authors.lastname: Li
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D018450:Disease Progression; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D012372:ROC Curve; D013345:Subarachnoid Hemorrhage / Q000097:blood* / Q000235:genetics
chemical_list: D015415:Biomarkers; C578622:MIRN1297 microRNA, human; C545730:MIRN502 microRNA, human; D035683:MicroRNAs
keywords: Biomarkers; MicroRNAs; Subarachnoid hemorrhage; qRT-PCR