title: Quantification of meropenem in human plasma by HILIC - tandem mass spectrometry.
abstract: Meropenem is a substance from the carbapenem class of antibiotics. It is widely used in intensive care units to treat a broad range of severe bacterial infections. In this critically ill patient group therapeutic drug monitoring (TDM) is highly advisable in order to avoid under- as well as over-dosing. Here, we describe a HILIC-MS/MS method for the quantification of meropenem in human plasma which is suitable for TDM. After simple protein precipitation, the polar analyte was chromatographically separated by the HILIC mechanism on a silica based stationary phase with polyethylenimide coating. The calibration was linear over the range of 1-50μg/ml. The stable isotope labelled internal standard led to matrix independency of the quantification and to very good precision (relative standard deviations less than 5%) and accuracy (deviations from the expected values less than 4%). A cross-validation with a validated and established HPLC-UV method confirmed the accuracy of the HILIC-MS/MS method in real patient samples.
authors.affiliation: Institute of Clinical Pharmacology, Otto-von-Guericke University, Magdeburg, Germany. Electronic address: jens.martens-lobenhoffer@med.ovgu.de.
authors.forename: Jens
authors.initials: J
authors.lastname: Martens-Lobenhoffer
authors.affiliation: Institute of Clinical Pharmacology, Otto-von-Guericke University, Magdeburg, Germany.
authors.forename: Stefanie M
authors.initials: SM
authors.lastname: Bode-Böger
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016903:Drug Monitoring / Q000379:methods*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D016014:Linear Models; D000077731:Meropenem; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013845:Thienamycins / Q000097:blood*
chemical_list: D013845:Thienamycins; D000077731:Meropenem
keywords: Human plasma; Hydrophilic interaction liquid chromatography; Meropenem; Tandem mass spectrometry; Therapeutic drug monitoring