title: PECAM-1 Leu125Val (rs688) Polymorphism and Diabetic Nephropathy in Caucasians with Type 2 Diabetes Mellitus.
abstract: Objectives. Platelet endothelial cell adhesion molecule-1 (PECAM-1) plays a key role in the transendothelial migration of circulating leukocytes during inflammation and in the maintenance of vascular endothelial integrity. We hypothesized that genetic variation inPECAM-1gene could be associated with diabetic nephropathy (DN) and with the level of soluble PECAM-1 in Caucasians with type 2 diabetes mellitus (T2DM).Design and Methods. We analyzed the rs688 single nucleotide polymorphism ofPECAM-1gene C373G (Leu125Val) at exon 3, which encodes the first extracellular Ig-like domain that mediates the homophilic binding of PECAM-1, in 276 T2DM subjects with documented DN (cases) and 375 T2DM subjects without DN (controls), using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) strategy. Level of plasma soluble PECAM-1 (sPECAM-1) was measured by ELISA in a subpopulation of 120 diabetics with DN.Results. We found no association between the Leu125Val polymorphism and DN in subjects with T2DM. Likewise, the Leu125Val polymorphism was not associated with serum sPECAM-1 levels in a subpopulation of 120 diabetics with DN.Conclusion. The Leu125Val polymorphism of PECAM-1 and the level of sPECAM-1 are not associated with DN in T2DM subjects of Slovenian origin.
authors.affiliation: University Medical Centre Maribor, Clinic for Internal Medicine, Department for Diabetes and Metabolic Diseases, Maribor, Slovenia.
authors.forename: Matej
authors.initials: M
authors.lastname: Završnik
authors.affiliation: General Hospital Izola, Department of Internal Medicine, Izola, Slovenia.
authors.forename: Stojan
authors.initials: S
authors.lastname: Kariž
authors.affiliation: General Hospital Slovenj Gradec, Department of Internal Medicine, Slovenj Gradec, Slovenia.
authors.forename: Jana
authors.initials: J
authors.lastname: Makuc
authors.affiliation: General Hospital Murska Sobota, Department of Internal Medicine, Murska Sobota, Slovenia.
authors.forename: Maja
authors.initials: M
authors.lastname: Šeruga
authors.affiliation: Faculty of Medicine, Institute of Histology and Embryology, University of Ljubljana, Ljubljana, Slovenia.
authors.forename: Ines
authors.initials: I
authors.lastname: Cilenšek
authors.affiliation: Faculty of Medicine, Institute of Histology and Embryology, University of Ljubljana, Ljubljana, Slovenia.
authors.forename: Daniel
authors.identifier: 0000-0001-6637-2542
authors.initials: D
authors.lastname: Petrovič
mesh_terms: D000368:Aged; D019943:Amino Acid Substitution; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000150:complications* / Q000235:genetics*; D003928:Diabetic Nephropathies / Q000097:blood / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D008875:Middle Aged; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000097:blood / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D017524:Slovenia; D044465:White People / Q000235:genetics
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1