title: Evaluation of rapid GeneXpert MTB/RIF method using DNA tissue specimens of vertebral bones in patients with suspected spondylitis TB.
abstract: OBJECTIVE
  To detectMycobacterium tuberculosisDNA and rifampicin resistance in vertebral bone tissue specimens from spondylitis TB suspects.
  
  
  RESULTS
  The rapid GeneXpert MTB/RIF and MGIT 960 liquid culture methods have been used in the specimens. Results from 70 suspects with spondylitis TB shown that 31.42% identified positive for spondylitis TB using culture method, while 88.57% shown positive results using rapid GeneXpert MTB/RIF method. The validity of GeneXpert MTB/RIF shown sensitivity value of 100%, specificity value of 16.6%, PPV of 35.48%, and NPV of 100%.
  
  
  CONCLUSIONS
  GeneXpert has a high sensitivity but low specificity value in this study.
authors.affiliation: Department of Medical Microbiology, Medical Faculty, Hasanuddin University, Makassar 90245, Indonesia.
authors.forename: Muhammad Nasrum
authors.initials: MN
authors.lastname: Massi
authors.affiliation: Department of Orthopedic and Traumotology, Medical Faculty, Hasanuddin University, Makassar 90245, Indonesia.
authors.forename: Karya Triko
authors.initials: KT
authors.lastname: Biatko
authors.affiliation: Clinical Pathology Laboratory, Wahidin Sudirohusodo Hospital, Makassar 90245, Indonesia.
authors.forename: Irda
authors.initials: I
authors.lastname: Handayani
authors.affiliation: Department of Medical Microbiology, Medical Faculty, Hasanuddin University, Makassar 90245, Indonesia.
authors.forename: Muhammad Yogi
authors.initials: MY
authors.lastname: Pratama
authors.affiliation: Medical Research Center, Medical Faculty, Hasanuddin University, Makassar 90245, Indonesia.
authors.forename: Sari
authors.initials: S
authors.lastname: Septriani
authors.affiliation: Medical Research Center, Medical Faculty, Hasanuddin University, Makassar 90245, Indonesia.
authors.forename: Gaby Maulida
authors.initials: GM
authors.lastname: Nurdin
authors.affiliation: Medical Research Center, Medical Faculty, Hasanuddin University, Makassar 90245, Indonesia.
authors.forename: Marina B
authors.initials: MB
authors.lastname: Ali
keywords: DNA M. tuberculosis; GeneXpert MTB/RIF; Specimen pus; Spondylitis TB