title: Mixing Block Copolymers with Phospholipids at the Nanoscale: From Hybrid Polymer/Lipid Wormlike Micelles to Vesicles Presenting Lipid Nanodomains.
abstract: Hybrids, i.e., intimately mixed polymer/phospholipid vesicles, can potentially marry in a single membrane the best characteristics of the two separate components. The ability of amphiphilic copolymers and phospholipids to self-assemble into hybrid membranes has been studied until now on the submicrometer scale using optical microscopy on giant hybrid unilamellar vesicles (GHUVs), but limited information is available on large hybrid unilamellar vesicles (LHUVs). In this work, copolymers based on poly(dimethylsiloxane) and poly(ethylene oxide) with different molar masses and architectures (graft, triblock) were associated with 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). Classical protocols of LUV formation were used to obtain nanosized self-assembled structures. Using small-angle neutron scattering (SANS), time-resolved Förster resonance energy transfer (TR-FRET), and cryo-transmission electron microscopy (cryo-TEM), we show that copolymer architecture and molar mass have direct influences on the formation of hybrid nanostructures that can range from wormlike hybrid micelles to hybrid vesicles presenting small lipid nanodomains.
authors.affiliation: University of Bordeaux , LCPO UMR 5629, 16 Avenue Pey Berland, F-33600 Pessac, France.|CNRS , Laboratoire de Chimie des Polymères Organiques, UMR 5629, F-33600, Pessac, France.|Centro de Química-Física Molecular and Institute of Nanoscience and Nanotechnology, Universidade de Lisboa Instituto Superior Técnico , 1049-001 Lisboa, Portugal.
authors.forename: T P Tuyen
authors.initials: TP
authors.lastname: Dao
authors.affiliation: Laboratoire Léon Brillouin, UMR12 CEA-CNRS, CEA Saclay , F-91191 Gif-sur-Yvette Cedex, France.
authors.forename: A
authors.initials: A
authors.lastname: Brûlet
authors.affiliation: Centro de Química-Física Molecular and Institute of Nanoscience and Nanotechnology, Universidade de Lisboa Instituto Superior Técnico , 1049-001 Lisboa, Portugal.|Research Unit on Applied Molecular Biosciences-Rede de Química e Tecnologia (UCIBIO-REQUIMTE), Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa , Caparica, Portugal.
authors.forename: F
authors.initials: F
authors.lastname: Fernandes
authors.affiliation: Institut Charles Sadron, UPR 22 CNRS, Université de Strasbourg , 23 rue du Loess, 67034 Strasbourg, France.
authors.forename: M
authors.initials: M
authors.lastname: Er-Rafik
authors.affiliation: University of Bordeaux , LCPO UMR 5629, 16 Avenue Pey Berland, F-33600 Pessac, France.|CNRS , Laboratoire de Chimie des Polymères Organiques, UMR 5629, F-33600, Pessac, France.
authors.forename: K
authors.identifier: 0000-0003-3073-9722
authors.initials: K
authors.lastname: Ferji
authors.affiliation: ILL Grenoble, DS LSS, CS20156, 71 Avenue Martyrs, F-38042 Grenoble 9, France.
authors.forename: R
authors.initials: R
authors.lastname: Schweins
authors.affiliation: Université de Bordeaux, CNRS , Ctr Rech Paul Pascal CRPP, UPR 8641, F-33600 Pessac, France.
authors.forename: J-P
authors.identifier: 0000-0002-1988-1288
authors.initials: JP
authors.lastname: Chapel
authors.affiliation: Centro de Química-Física Molecular and Institute of Nanoscience and Nanotechnology, Universidade de Lisboa Instituto Superior Técnico , 1049-001 Lisboa, Portugal.
authors.forename: A
authors.initials: A
authors.lastname: Fedorov
authors.affiliation: Institut Charles Sadron, UPR 22 CNRS, Université de Strasbourg , 23 rue du Loess, 67034 Strasbourg, France.
authors.forename: M
authors.initials: M
authors.lastname: Schmutz
authors.affiliation: Centro de Química-Física Molecular and Institute of Nanoscience and Nanotechnology, Universidade de Lisboa Instituto Superior Técnico , 1049-001 Lisboa, Portugal.
authors.forename: M
authors.initials: M
authors.lastname: Prieto
authors.affiliation: University of Bordeaux , LCPO UMR 5629, 16 Avenue Pey Berland, F-33600 Pessac, France.|CNRS , Laboratoire de Chimie des Polymères Organiques, UMR 5629, F-33600, Pessac, France.
authors.forename: O
authors.identifier: 0000-0002-1815-2702
authors.initials: O
authors.lastname: Sandre
authors.affiliation: University of Bordeaux , LCPO UMR 5629, 16 Avenue Pey Berland, F-33600 Pessac, France.|CNRS , Laboratoire de Chimie des Polymères Organiques, UMR 5629, F-33600, Pessac, France.
authors.forename: J-F
authors.identifier: 0000-0003-0922-7407
authors.initials: JF
authors.lastname: Le Meins
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D008823:Micelles; D008958:Models, Molecular; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D053835:Unilamellar Liposomes / Q000737:chemistry*
chemical_list: D004129:Dimethylpolysiloxanes; D008823:Micelles; D053835:Unilamellar Liposomes; D015060:1,2-Dipalmitoylphosphatidylcholine; D011092:Polyethylene Glycols; C013830:baysilon