title: Brucella abortus 2308ΔNodVΔNodW double-mutant is highly attenuated and confers protection against wild-type challenge in BALB/c mice.
abstract: Brucellosis is an important zoonotic disease of worldwide distribution, which causes animal and human disease. However, the current Brucella abortus (B. abortus) vaccines (S19 and RB51) have several drawbacks, including residual virulence for animals and humans. Moreover, S19 cannot allow serological differentiation between infected and vaccinated animals. We constructed double deletion (ΔNodVΔNodW) mutant from virulent B. abortus 2308 (S2308) by deleting the genes encoding two-component regulatory system (TCS) in chromosome II in S2308.2308ΔNodVΔNodW was significantly reduced survival in murine macrophages (RAW 264.7) and BALB/c mice. Moreover, the inoculated mice showed no splenomegaly. The mutant induced high protective immunity in BALB/c mice against challenge with S2308, and elicited an anti-Brucella-specific immunoglobulin G (IgG) response and induced the secretion of gamma interferon (IFN-γ) and interleukin-4 (IL-4). Moreover, NODV and NODW antigens would allow the serological differentiation between infected and vaccinated animals. These results suggest that 2308ΔNodVΔNodW mutant is a potential live attenuated vaccine candidate and can be used effectively against bovine brucellosis.
authors.affiliation: School of Biotechnology and Food, Shangqiu Normal University, Shangqiu 476000, Henan, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Li
authors.affiliation: School of Biotechnology and Food, Shangqiu Normal University, Shangqiu 476000, Henan, China.
authors.forename: Shuli
authors.initials: S
authors.lastname: Wang
authors.affiliation: School of Biotechnology and Food, Shangqiu Normal University, Shangqiu 476000, Henan, China.
authors.forename: Jinliang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Biotechnology and Food, Shangqiu Normal University, Shangqiu 476000, Henan, China.
authors.forename: Guangli
authors.initials: G
authors.lastname: Yang
authors.affiliation: School of Biotechnology and Food, Shangqiu Normal University, Shangqiu 476000, Henan, China.
authors.forename: Baodong
authors.initials: B
authors.lastname: Yuan
authors.affiliation: School of Biotechnology and Food, Shangqiu Normal University, Shangqiu 476000, Henan, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Huang
authors.affiliation: School of Biotechnology and Food, Shangqiu Normal University, Shangqiu 476000, Henan, China.
authors.forename: Jincheng
authors.initials: J
authors.lastname: Han
authors.affiliation: School of Biotechnology and Food, Shangqiu Normal University, Shangqiu 476000, Henan, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xi
authors.affiliation: Institution of Forestry Bureau, Nangong 055750, Hebei, China.
authors.forename: Yanren
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, China.
authors.forename: Chuangfu
authors.initials: C
authors.lastname: Chen
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, China. Electronic address: allanzhh@sohu.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D002004:Brucella Vaccine / Q000235:genetics / Q000276:immunology*; D002003:Brucella abortus / Q000235:genetics* / Q000276:immunology*; D002006:Brucellosis / Q000097:blood / Q000276:immunology* / Q000517:prevention & control*; D002007:Brucellosis, Bovine / Q000517:prevention & control; D002417:Cattle; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D017353:Gene Deletion; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D015847:Interleukin-4 / Q000276:immunology / Q000378:metabolism; D008264:Macrophages / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D000067996:RAW 264.7 Cells; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D013154:Spleen / Q000276:immunology; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology*; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics
chemical_list: D000907:Antibodies, Bacterial; D002004:Brucella Vaccine; D016207:Cytokines; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D014613:Vaccines, Attenuated; D037521:Virulence Factors; D015847:Interleukin-4; D007371:Interferon-gamma
keywords: B. abortus 2308; Live attenuated vaccine; NodV; NodW