title: Heat-killed cell preparation of Corynebacterium glutamicum stimulates the immune activity and improves survival of mice against enterohemorrhagic Escherichia coli.
abstract: Fermentation by Corynebacterium glutamicum is used by various industries to produce L-Glutamate, and the heat-killed cell preparation of this bacterium (HCCG) is a by-product of the fermentation process. In present study, we evaluated the immunostimulating and survival effects against enterohemorrhagic Escherichia coli (STEC) infection of HCCG. HCCG significantly stimulated in vitro IgA and interleukin-12 p70 production in murine Peyer's patch cells and peritoneal macrophages, respectively. Oral administration of 10 mg/kg body weight (BW) of HCCG for seven consecutive days stimulated IgA concentration in murine cecal digesta. Mice were orally administered HCCG for 17 consecutive days (d0-d17), and challenged with STEC on d4 to d6. Survival of mice tended to improve by 100 mg/kg BW of HCCG administration compared with those in control group. In conclusion, HCCG supplementation was found to prevent STEC infection in mice, and thus it may have the potential to stimulate the immune status of mammals.
authors.affiliation: a Ajinomoto Co., Inc. , Kawasaki , Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Ebisawa
authors.affiliation: b Kyoto Institute of Nutrition & Pathology , Ujitawara , Japan.
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Tsukahara
authors.affiliation: a Ajinomoto Co., Inc. , Kawasaki , Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Fudou
authors.affiliation: a Ajinomoto Co., Inc. , Kawasaki , Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Ohta
authors.affiliation: a Ajinomoto Co., Inc. , Kawasaki , Japan.
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Tokura
authors.affiliation: a Ajinomoto Co., Inc. , Kawasaki , Japan.
authors.forename: Norimasa
authors.initials: N
authors.lastname: Onishi
authors.affiliation: a Ajinomoto Co., Inc. , Kawasaki , Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Fujieda
mesh_terms: D000818:Animals; D048230:Corynebacterium glutamicum / Q000166:cytology*; D003967:Diarrhea / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology* / Q000517:prevention & control; D054324:Enterohemorrhagic Escherichia coli / Q000502:physiology*; D006358:Hot Temperature; D007070:Immunoglobulin A / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D051379:Mice; D016019:Survival Analysis
chemical_list: D007070:Immunoglobulin A; D018664:Interleukin-12
keywords: Shiga toxin 2e; heat-killed cell preparation of Corynebacterium glutamicum; immunoglobulin A; interleukin 12; mouse model