title: Lnc‑IL7R promotes the growth of fibroblast‑like synoviocytes through interaction with enhancer of zeste homolog 2 in rheumatoid arthritis.
abstract: Rheumatoid arthritis (RA) is an inflammatory and autoimmune disease that affects ~1% of the world's population. Although the precise mechanism of RA has yet to be elucidated, accumulating evidence suggests that fibroblast‑like synoviocytes (FLSs) serve critical roles in the initiation and progression of RA. However, the underlying molecular mechanisms of FLS proliferation have yet to be elucidated. Long noncoding‑interleukin‑7 receptor (lnc‑IL7R) has been recently identified, which is activated by lipopolysaccharide (LPS) stimulation and diminishes the LPS‑induced inflammatory response. In the present study, gain‑ and loss‑of‑function assays were performed in order to investigate the role of lnc‑IL7R in FLS. It is demonstrated, to the best of the authors' knowledge for the first time, that lnc‑IL7R promotes cell proliferation, cell cycle progression and inhibits apoptosis in FLS. Further investigation identified that lnc‑IL7 interacts with enhancer of zeste homolog 2 (EZH2) and is required for polycomb repressive complex 2 (PRC2)‑mediated suppression, including cyclin‑dependent kinase inhibitor 1A and cyclin‑dependent kinase inhibitor 2A. Lnc‑IL7R may be a promising therapeutic target for the treatment of RA.
authors.affiliation: Department of Orthopedics, Cangzhou Central Hospital, Cangzhou, Hebei 061000, P.R. China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Ye
authors.affiliation: Department of Ultrasound, Cangzhou Central Hospital, Cangzhou, Hebei 061000, P.R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Orthopedics, Cangzhou Central Hospital, Cangzhou, Hebei 061000, P.R. China.
authors.forename: Shukui
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Cangzhou Central Hospital, Cangzhou, Hebei 061000, P.R. China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Cai
authors.affiliation: Department of Teaching, Cangzhou Central Hospital, Cangzhou, Hebei 061000, P.R. China.
authors.forename: Tiejun
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Cangzhou Central Hospital, Cangzhou, Hebei 061000, P.R. China.
authors.forename: Lishan
authors.initials: L
authors.lastname: Sun
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001172:Arthritis, Rheumatoid / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D002453:Cell Cycle / Q000235:genetics; D049109:Cell Proliferation; D002478:Cells, Cultured; D000071221:Enhancer of Zeste Homolog 2 Protein / Q000235:genetics* / Q000378:metabolism; D044127:Epigenesis, Genetic; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D006801:Humans; D062085:RNA, Long Noncoding / Q000235:genetics*; D020395:Receptors, Interleukin-7 / Q000235:genetics* / Q000378:metabolism; D000070918:Synoviocytes / Q000378:metabolism*
chemical_list: D062085:RNA, Long Noncoding; D020395:Receptors, Interleukin-7; D000071221:Enhancer of Zeste Homolog 2 Protein