title: Proteomics-based analysis of lung injury-induced proteins in a mouse model of common bile duct ligation.
abstract: Lung injury is a life-threatening complication in patients with liver dysfunction. We recently provided an experimental lung injury model in mouse with common bile duct ligation. In this study, we aimed to characterize the pathologic and biochemical features of lung tissues in common bile duct ligation mice using a proteomic approach.
  
  
  
  Common bile ducts of BALB/c mice, 8 weeks of age, were ligated operatively. CD31-expressing pulmonary cells were sorted with immunomagnetic microbeads, and protein profiles were examined by 2-dimensional gel electrophoresis. Based on the results of protein identification, immunohistochemistry and quantitative reverse transcription polymerase chain reaction were carried out in pulmonary and hepatic tissues.
  
  
  
  Two-dimensional gel electrophoresis revealed 3 major inflammation-associated proteins exhibiting considerable increases in the number of CD31-positive pulmonary cells after common bile duct ligation. Mass spectrometry analysis identified these proteins as SerpinB1a (48 kDa), ANXA1 (46 kDa), and S100A9 (16 kDa). Furthermore, the 3 proteins were more highly expressed in dilated pulmonary blood vessels of common bile duct ligation mice, in which neutrophils and monocytes were prominent, as shown by immunohistochemistry. More importantly, SerpinB1a mRNA and protein were significantly upregulated in the liver, whereas S100A9 and ANXA1 mRNA and protein were upregulated in the lungs, as shown by quantitative reverse transcription polymerase chain reaction and Western blotting.
  
  
  
  We identified 3 proteins that were highly expressed in the lung after common bile duct ligation using a proteomics-based approach.
authors.affiliation: Department of Cardiovascular and Thoracic Surgery, Ehime University Graduate School of Medicine, Toon, Ehime, Japan; Department of Cell Growth and Tumor Regulation, Proteo-Science Center (PROS), Toon, Ehime, Japan.
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Sakaue
authors.affiliation: Department of Cardiovascular and Thoracic Surgery, Ehime University Graduate School of Medicine, Toon, Ehime, Japan; Department of Cardiothoracic Surgery, St Vincent's Hospital Sydney, NSW, Australia.
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Shikata
authors.affiliation: Department of Biochemistry and Molecular Genetics, Ehime University Graduate School of Medicine, Toon, Ehime, Japan.
authors.forename: Kaho
authors.initials: K
authors.lastname: Utsunomiya
authors.affiliation: Department of Biochemistry and Molecular Genetics, Ehime University Graduate School of Medicine, Toon, Ehime, Japan.
authors.forename: Shunya
authors.initials: S
authors.lastname: Fukae
authors.affiliation: Department of Pathology, Division of Analytical Pathology, Ehime University Graduate School of Medicine, Toon, Ehime, Japan; Department of Pathology, Proteo-Science Center (PROS), Toon, Ehime, Japan.
authors.forename: Mie
authors.initials: M
authors.lastname: Kurata
authors.affiliation: Department of Cardiovascular and Thoracic Surgery, Ehime University Graduate School of Medicine, Toon, Ehime, Japan.
authors.forename: Hirotomo
authors.initials: H
authors.lastname: Nakaoka
authors.affiliation: Department of Cardiovascular and Thoracic Surgery, Ehime University Graduate School of Medicine, Toon, Ehime, Japan.
authors.forename: Mikio
authors.initials: M
authors.lastname: Okazaki
authors.affiliation: Department of Cardiothoracic Surgery, St Vincent's Hospital Sydney, NSW, Australia.
authors.forename: Yujiro
authors.initials: Y
authors.lastname: Kawanishi
authors.affiliation: Department of Cardiovascular and Thoracic Surgery, Ehime University Graduate School of Medicine, Toon, Ehime, Japan.
authors.forename: Ai
authors.initials: A
authors.lastname: Kojima
authors.affiliation: Department of Cell Growth and Tumor Regulation, Proteo-Science Center (PROS), Toon, Ehime, Japan; Department of Biochemistry and Molecular Genetics, Ehime University Graduate School of Medicine, Toon, Ehime, Japan.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Higashiyama
authors.affiliation: Department of Cardiovascular and Thoracic Surgery, Ehime University Graduate School of Medicine, Toon, Ehime, Japan. Electronic address: izutani@m.ehime-u.ac.jp.
authors.forename: Hironori
authors.initials: H
authors.lastname: Izutani
mesh_terms: D055371:Acute Lung Injury / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D017305:Annexin A1 / Q000032:analysis*; D015153:Blotting, Western; D040502:Calgranulin B / Q000032:analysis*; D003135:Common Bile Duct / Q000601:surgery*; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D020065:Hepatopulmonary Syndrome / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D008026:Ligation / Q000009:adverse effects* / Q000379:methods; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D040901:Proteomics; D011897:Random Allocation; D012680:Sensitivity and Specificity; D015843:Serpins / Q000032:analysis*
chemical_list: D017305:Annexin A1; D040502:Calgranulin B; C522950:Serpinb1a protein, mouse; D015843:Serpins; C573274:annexin A1, mouse