title: Antioxidative activities and phenolic compounds of pumpkin (Cucurbita pepo) seeds and amaranth (Amaranthus caudatus) grain extracts.
abstract: Phenolic compounds were extracted from pumpkin (Cucurbita pepo) seed and amaranth (Amaranthus caudatus) grain into 80% (v/v) methanol. The extracts obtained were characterised by the contents of total phenolic compounds (TPC), trolox equivalent antioxidant capacity (TEAC), ferric-reducing antioxidant power (FRAP) and antiradical activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH·) radical. The content of individual phenolic compounds was determined by HPLC-DAD method. Pumpkin seeds showed the higher content of TPC than that from amaranth. The TEAC values of both extracts were similar each other. The lower value of FRAP was observed for pumpkin seed. Phenolic compound present in amaranth grain exhibited strongest antiradical properties against DPPH radical. Several peaks were present on the HPLC chromatograms of two extracts. The UV-DAD spectra confirmed the presence of vanillic acid derivatives in the amaranth grain. The three main phenolic compound present in pumpkin seed were characterised by UV-DAD spectra with maximum at 258, 266 and 278 nm.
authors.affiliation: a Institute of Sciences of Food Production , National Research Council , Grugliasco , Italy.
authors.forename: Pier Giorgio
authors.initials: PG
authors.lastname: Peiretti
authors.affiliation: b Department of Veterinary Science , University of Torino , Grugliasco , Italy.
authors.forename: Giorgia
authors.initials: G
authors.lastname: Meineri
authors.affiliation: a Institute of Sciences of Food Production , National Research Council , Grugliasco , Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Gai
authors.affiliation: b Department of Veterinary Science , University of Torino , Grugliasco , Italy.
authors.forename: Erica
authors.initials: E
authors.lastname: Longato
authors.affiliation: c Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences , Olsztyn , Poland.
authors.forename: Ryszard
authors.initials: R
authors.lastname: Amarowicz
mesh_terms: D027721:Amaranthus / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001713:Biphenyl Compounds; D002851:Chromatography, High Pressure Liquid; D028464:Cucurbita / Q000737:chemistry*; D010636:Phenols / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D010851:Picrates; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry*; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: ABTS; DPPH; FRAP; Pumpkin; amaranth; antioxidant activity; phenolic compounds