title: Suppression of EIF4G2 by miR-379 potentiates the cisplatin chemosensitivity in nonsmall cell lung cancer cells.
abstract: Although microRNAs and EIF4G2 are both known to play pivotal roles in cancer progression, it remains unknown whether these pathways regulate chemosensitivity in a coordinated manner. Here, we show that miR-379 expression is significantly downregulated in chemoresistant nonsmall cell lung cancer (NSCLC) tissues and cells. Manipulation of miR-379 levels could alter the in vitro and in vivo cisplatin (CDDP) resistance in lung cancer (LCa) cells. Mechanistically, miR-379 potentiated LCa chemosensitivity via modulation of CDDP-induced apoptosis by directly targeting the EIF4G2 3'UTR. Additionally, we observed an inverse correlation between miR-379 and EIF4G2 expression in LCa tissues from patients with CDDP-based chemotherapy. Together, our findings shed new light on the potential involvement of miR-379/EIF4G2 cascade in the pathogenesis of CDDP resistance in LCa.
authors.affiliation: Department of Oncology, First Hospital of Yulin City, China.
authors.forename: Guang-Jun
authors.initials: GJ
authors.lastname: Hao
authors.affiliation: Department of Clinical Laboratory, First Hospital of Yulin City, China.
authors.forename: Hai-Jun
authors.initials: HJ
authors.lastname: Hao
authors.affiliation: Department of Oncology, First Hospital of Yulin City, China.
authors.forename: Yan-Hui
authors.initials: YH
authors.lastname: Ding
authors.affiliation: Department of Oncology, First Hospital of Yulin City, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wen
authors.affiliation: Department of Oncology, First Hospital of Yulin City, China.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Li
authors.affiliation: Department of Oncology, First Hospital of Yulin City, China.
authors.forename: Qian-Ru
authors.initials: QR
authors.lastname: Wang
authors.affiliation: Department of Oncology, First Hospital of Yulin City, China.
authors.forename: Bing-Bing
authors.initials: BB
authors.lastname: Zhang
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000072283:A549 Cells; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D001483:Base Sequence; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology*; D039603:Eukaryotic Initiation Factor-4G / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006801:Humans; D015151:Immunoblotting; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D023041:Xenograft Model Antitumor Assays
chemical_list: D020413:3' Untranslated Regions; D000970:Antineoplastic Agents; C491300:EIF4G2 protein, human; D039603:Eukaryotic Initiation Factor-4G; C561022:MIRN379 microRNA, human; D035683:MicroRNAs; D002945:Cisplatin
keywords: EIF4F; chemoresistance; cisplatin; lung cancer; miRNAs