title: The dental resin monomers HEMA and TEGDMA have inhibitory effects on osteoclast differentiation with low cytotoxicity.
abstract: The dental resin monomers 2-hydroxyethyl methacrylate (HEMA) and triethylene glycol dimethacrylate (TEGDMA) are released from the resin matrix due to unpolymerized monomers; once released, they influence various biological functions and the viability of cells in the oral environment. Although HEMA and TEGDMA have various effects on cells, including inflammation, inhibition of cell proliferation or differentiation, and apoptosis, the effects of these monomers on osteoclasts remain unknown. In this study, we investigated the effects of HEMA and TEGDMA on osteoclast differentiation of bone marrow-derived macrophages or murine monocytic cell line RAW-D. Both HEMA and TEGDMA inhibited osteoclast formation and their bone-resorbing activity at non-cytotoxic concentrations. Moreover, HEMA and TEGDMA decreased the expression of nuclear factor of activated T cells cytoplasmic-1 (NFATc1), a master regulator of osteoclast differentiation, and of osteoclast markers that are transcriptionally regulated by NFATc1, including Src and cathepsin K. Regarding their effects on signaling pathways involved in osteoclast differentiation, HEMA impaired the phosphorylation of extracellular signal-regulated kinase and Jun N-terminal kinase, whereas TEGDMA attenuated the phosphorylation of Akt and Jun N-terminal kinase. Thus, HEMA and TEGDMA inhibit osteoclast differentiation through different signaling pathways. This is the first report on the effects of the monomers HEMA and TEGDMA on osteoclasts. Copyright © 2017 John Wiley & Sons, Ltd.
authors.affiliation: Department of Dental Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, Sakamoto 1-7-1, Nagasaki, 852-8588, Japan.|Department of Prosthetic Dentistry, Nagasaki University Graduate School of Biomedical Sciences, Sakamoto 1-7-1, Nagasaki, 852-8588, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Inamitsu
authors.affiliation: Department of Dental Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, Sakamoto 1-7-1, Nagasaki, 852-8588, Japan.
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Okamoto
authors.affiliation: Department of Dental Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, Sakamoto 1-7-1, Nagasaki, 852-8588, Japan.
authors.forename: Eiko
authors.initials: E
authors.lastname: Sakai
authors.affiliation: Department of Dental Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, Sakamoto 1-7-1, Nagasaki, 852-8588, Japan.
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Nishishita
authors.affiliation: Department of Prosthetic Dentistry, Nagasaki University Graduate School of Biomedical Sciences, Sakamoto 1-7-1, Nagasaki, 852-8588, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Murata
authors.affiliation: Department of Dental Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, Sakamoto 1-7-1, Nagasaki, 852-8588, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Tsukuba
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D003603:Cytotoxins / Q000009:adverse effects*; D006801:Humans; D008689:Methacrylates / Q000009:adverse effects*; D051379:Mice; D010010:Osteoclasts / Q000187:drug effects*; D011092:Polyethylene Glycols / Q000009:adverse effects*; D011109:Polymethacrylic Acids / Q000009:adverse effects*; D012117:Resins, Synthetic / Q000009:adverse effects*
chemical_list: D003603:Cytotoxins; D008689:Methacrylates; D011109:Polymethacrylic Acids; D012117:Resins, Synthetic; C020946:triethylene glycol dimethacrylate; D011092:Polyethylene Glycols; C005044:hydroxyethyl methacrylate
keywords: HEMA; TEGDMA; osteoclasts; cytotoxicity