title: Establishment of Proliferative Tetraploid Cells from Nontransformed Human Fibroblasts.
abstract: Polyploid (mostly tetraploid) cells are often observed in preneoplastic lesions of human tissues and their chromosomal instability has been considered to be responsible for carcinogenesis in such tissues. Although proliferative polyploid cells are requisite for analyzing chromosomal instability of polyploid cells, creating such cells from nontransformed human cells is rather challenging. Induction of tetraploidy by chemical agents usually results in a mixture of diploid and tetraploid populations, and most studies employed fluorescence-activated cell sorting or cloning by limiting dilution to separate tetraploid from diploid cells. However, these procedures are time-consuming and laborious. The present report describes a relatively simple protocol to induce proliferative tetraploid cells from normal human fibroblasts with minimum contamination by diploid cells. Briefly, the protocol is comprised of the following steps: arresting cells in mitosis by demecolcine (DC), collecting mitotic cells after shaking off, incubating collected cells with DC for an additional 3 days, and incubating cells in drug-free medium (They resume proliferation as tetraploid cells within several days). Depending on cell type, the collection of mitotic cells by shaking off might be omitted. This protocol provides a simple and feasible method to establish proliferative tetraploid cells from normal human fibroblasts. Tetraploid cells established by this method could be a useful model for studying chromosome instability and the oncogenic potential of polyploid human cells.
authors.affiliation: Division of Morphological Science, Biomedical Research Center, Saitama Medical University; sohshima@saitama-med.ac.jp.
authors.forename: Susumu
authors.initials: S
authors.lastname: Ohshima
authors.affiliation: Department of Pathology, International Medical Center, Saitama Medical University.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Seyama
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation; D043171:Chromosomal Instability; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism; D003703:Demecolcine / Q000494:pharmacology; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007621:Karyotyping; D008938:Mitosis / Q000187:drug effects; D057891:Tetraploidy
chemical_list: D005456:Fluorescent Dyes; D004247:DNA; D003703:Demecolcine