title: Effect of platelet-rich plasma on reconstruction with nerve autografts.
abstract: Despite advances in understanding of peripheral nerve injuries and regeneration and advances in surgical techniques, successful outcomes cannot be guaranteed after reconstructive surgery. Platelet-rich plasma (PRP) has been reported to have positive effects on nerve regeneration, as well as on tissue healing. The present study was designed to evaluate the effect of PRP on nerve-grafted defects. Sprague-Dawley rats were divided into four surgery groups (n=7 in each). A 1-cm long nerve defect was created in the upper thigh and then reconstructed using a nerve autograft in all groups. The wet muscle weights, electromyographic findings, and histomorphologic changes were evaluated 10 weeks later. As shown by both the electromyographic (p<0.001) and histomorphologic findings (p<0.001), PRP had more positive effects on nerve gap reconstruction in Group 3 then Group 4 as compared to the control groups. The present study is novel in that it evaluated the regeneration effect of PRP on a large nerve defect reconstructed with a nerve graft rather than primary repair. The results are encouraging for further experimental studies on the role of PRP in nerve healing.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Ege University School of Medicine, Izmir, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Teymur
authors.affiliation: Department of Plastic and Reconstructive Surgery, Ege University School of Medicine, Izmir, Turkey.
authors.forename: Yigit Ozer
authors.initials: YO
authors.lastname: Tiftikcioglu
authors.affiliation: Department of Histology and Embryology, Ege University School of Medicine, Izmir, Turkey; Cord blood, Cell, and Tissue Research and Application Center, Ege University, Izmir, Turkey.
authors.forename: Turker
authors.initials: T
authors.lastname: Cavusoglu
authors.affiliation: Department of Neurology, Ministry of Health Tepecik Education and Research Hospital, Izmir, Turkey.
authors.forename: Bedile Irem
authors.initials: BI
authors.lastname: Tiftikcioglu
authors.affiliation: Department of Physiology, Faculty of Medicine, Istanbul Bilim University, Istanbul, Turkey.
authors.forename: Oytun
authors.initials: O
authors.lastname: Erbas
authors.affiliation: Department of Histology and Embryology, Ege University School of Medicine, Izmir, Turkey.
authors.forename: Gurkan
authors.initials: G
authors.lastname: Yigitturk
authors.affiliation: Department of Histology and Embryology, Ege University School of Medicine, Izmir, Turkey; Cord blood, Cell, and Tissue Research and Application Center, Ege University, Izmir, Turkey. Electronic address: yigit.uyanikgil@ege.edu.tr.
authors.forename: Yigit
authors.initials: Y
authors.lastname: Uyanikgil
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000818:Animals; D064592:Autografts; D004576:Electromyography; D005355:Fibrosis / Q000517:prevention & control; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D018482:Muscle, Skeletal / Q000293:injuries / Q000294:innervation / Q000601:surgery; D009416:Nerve Regeneration / Q000187:drug effects* / Q000502:physiology; D053657:Platelet-Rich Plasma / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000187:drug effects*; D012584:Sciatic Nerve / Q000187:drug effects* / Q000293:injuries / Q000601:surgery / Q000637:transplantation; D013536:Suture Techniques; D013537:Sutures
chemical_list: D036341:Intercellular Signaling Peptides and Proteins
keywords: Microsurgery; Nerve regeneration; Platelet-rich plasma