title: Evaluation of ROS-TAC Score and DNA Damage in Fertile Normozoospermic and Infertile Asthenozoospermic Males.
abstract: OBJECTIVE
  The aim of study was to evaluate reactive oxygen species (ROS), total antioxidant capacity (TAC) andROS-TAC score as indicator for oxidative stress status as well as 8-hydrodeoxyguanosine (8-OHdG) levels as amarker for DNA damage in the seminal plasma of asthenozoospermia patients compared to normozoospermiasamples.
  
  
  METHODS
  The semen samples of 28 fertile normozoospermic donors and 25 infertile men withasthenozoospermia were analyzed according to World Health Organization (WHO) criteria. ROS production wasmeasured in neat semen samples by the chemiluminescent assay. Plasma levels of TAC was measured by commercially available colorimetric assays. The levels of DNA oxidative damage were measured by seminal plasma levelsof 8-OHdG using ELISA method. ROS-TAC score was measured using principal component analysis.
  
  
  RESULTS
  Asthenozoospermic men had a higher ROS levels compared to the normozoospermic men (P = .01).However, no significant difference was observed in TAC levels between the groups. ROS-TAC score in asthenozoospermicmen was lower than normozoospermic men (P = .02). The levels of 8-OHdG in the asthenozoospermicmen were higher than normozoospermic men (P = .01).
  
  
  CONCLUSIONS
  The present study demonstrated a decrease in ROS-TAC score and, a high DNA damage in asthenozoospermiacompared to normozoospermia. ROS-TAC score can predict the oxidative damage of semen samplesof astenozoospermic infertile males.
authors.affiliation: Department of Clinical Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Akram
authors.initials: A
authors.lastname: Vatannejad
authors.affiliation: Urology and nephrology research center, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Heidar
authors.initials: H
authors.lastname: Tavilani
authors.affiliation: Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Sadeghi
authors.affiliation: Cancer biology research Centre, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Saeid
authors.initials: S
authors.lastname: Amanpour
authors.affiliation: Payame Noor University, Tehran, Iran.
authors.forename: Somyeh
authors.initials: S
authors.lastname: Shapourizadeh
authors.affiliation: Department of Clinical Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. doostimd@sina.tums.ac.ir.
authors.forename: Mahmood
authors.initials: M
authors.lastname: Doosti
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D053627:Asthenozoospermia / Q000235:genetics* / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis; D005298:Fertility / Q000235:genetics; D006801:Humans; D007248:Infertility, Male / Q000235:genetics / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress; D017382:Reactive Oxygen Species; D012661:Semen
chemical_list: D015415:Biomarkers; D017382:Reactive Oxygen Species; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine