title: Comment on 'A novel method for fast and robust estimation of fluorescence decay dynamics using constrained least-square deconvolution with Laguerre expansion'.
abstract: This comment is to clarify that Poisson noise instead of Gaussian noise shall be included to assess the performances of least-squares deconvolution with Laguerre expansion (LSD-LE) for analysing fluorescence lifetime imaging data obtained from time-resolved systems. Moreover, we also corrected an equation in the paper. As the LSD-LE method is rapid and has the potential to be widely applied not only for diagnostic but for wider bioimaging applications, it is desirable to have precise noise models and equations.
authors.affiliation: Centre for Biophotonics, Strathclyde Institute of Pharmacy & Biomedical Sciences, University of Strathclyde, Glasgow, Scotland, UK. College of Information Science & Electronic Engineering, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Zhang
authors.forename: David Day-Uei
authors.initials: DD
authors.lastname: Li
mesh_terms: D016018:Least-Squares Analysis; D008856:Microscopy, Fluorescence; D016011:Normal Distribution