title: Age-related changes in pre- and post-conization HPV genotype distribution among women with high-grade cervical intraepithelial neoplasia.
abstract: OBJECTIVE
  To assess the effect of age on pre- and post-conization HPV genotype distribution.
  
  
  METHODS
  The present retrospective observational study included consecutive women with high-grade cervical intraepithelial neoplasia who underwent conization at the Cervical Cancer Screening Centre of Reggio Emilia, Italy, and University Hospital of Modena, Italy, between February 1, 2012, and October 31, 2014. Pre-conization and 6-month post-conization HPV genotyping results were compared between four age groups (<30, 30-39, 40-49, and ≥50 years) and age-related changes in the HPV genotypes present were evaluated.
  
  
  RESULTS
  There were 162 patients included. The lowest occurrence of pre-conization high-risk and probable high-risk HPV genotypes was observed among patients aged at least 50 years when compared with younger patients (P=0.017). Conversely, women aged at least 50 years exhibited the highest level of post-conization high-risk and probable high-risk HPV genotypes (P=0.043). Additionally, an increasing incidence of recording identical pre- and post-conization HPV genotypes was associated with increasing age (P=0.024), as was increasing post-treatment recurrence of cervical intraepithelial neoplasia grade 2+ (P=0.030).
  
  
  CONCLUSIONS
  The presence of high-risk and probable high-risk HPV genotypes was lowest among older patients before conization and was highest among these patients post-conization; post-treatment HPV clearance decreased with age and increasing age could be a risk factor for post-conization recurrence.
authors.affiliation: Local Health Authority of Reggio Emilia, Division of Obstetrics and Gynecology, Cesare Magati Hospital, Scandiano, Italy.|Cervical Cancer Screening Centre, IRCCS-Arcispedale Santa Maria Nuova, Reggio Emilia, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Giannella
authors.affiliation: Cervical Cancer Screening Centre, IRCCS-Arcispedale Santa Maria Nuova, Reggio Emilia, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Fodero
authors.affiliation: Mother-Infant Department, Institute of Obstetrics and Gynecology, University of Modena and Reggio Emilia, Modena, Italy.
authors.forename: Fausto
authors.initials: F
authors.lastname: Boselli
authors.affiliation: Cervical Cancer Screening Centre, IRCCS-Arcispedale Santa Maria Nuova, Reggio Emilia, Italy.
authors.forename: Teresa
authors.initials: T
authors.lastname: Rubino
authors.affiliation: Local Health Authority of Reggio Emilia, Division of Obstetrics and Gynecology, Cesare Magati Hospital, Scandiano, Italy.|Cervical Cancer Screening Centre, IRCCS-Arcispedale Santa Maria Nuova, Reggio Emilia, Italy.
authors.forename: Kabala
authors.initials: K
authors.lastname: Mfuta
authors.affiliation: Cervical Cancer Screening Centre, IRCCS-Arcispedale Santa Maria Nuova, Reggio Emilia, Italy.
authors.forename: Sonia
authors.initials: S
authors.lastname: Prandi
mesh_terms: D000328:Adult; D000367:Age Factors; D019092:Conization / Q000379:methods; D005260:Female; D005838:Genotype; D006801:Humans; D000068884:Immunosenescence; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000209:etiology; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000821:virology*; D012189:Retrospective Studies; D012307:Risk Factors; D002583:Uterine Cervical Neoplasms / Q000601:surgery*; D002578:Uterine Cervical Dysplasia / Q000601:surgery*
keywords: Age; Cervical intraepithelial neoplasia; Conization; Human papillomavirus