title: Isolation and Culture of Vascular Smooth Muscle Cells from Small and Large Vessels.
abstract: Primary culture of vascular smooth muscle cells is an important in vitro model for the dissection of molecular mechanisms related to a specific physiological or pathological response at the cellular level. Cultured cells also provide an excellent model to study cell biology. This chapter describes a user-friendly and practical protocol for isolation of vascular smooth muscle cells from small and large vessels by enzymatic dissociation, which can be applied to vessels from different species, including rodents and humans.
authors.affiliation: Institute of Cardiovascular and Medical Sciences BHF Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, UK. Augusto.montezano@glasgow.ac.uk.
authors.forename: Augusto C
authors.initials: AC
authors.lastname: Montezano
authors.affiliation: Institute of Cardiovascular and Medical Sciences BHF Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, UK.
authors.forename: Rheure A M
authors.initials: RA
authors.lastname: Lopes
authors.affiliation: Institute of Cardiovascular and Medical Sciences BHF Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, UK.
authors.forename: Karla B
authors.initials: KB
authors.lastname: Neves
authors.affiliation: Institute of Cardiovascular and Medical Sciences BHF Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, UK.
authors.forename: Francisco
authors.initials: F
authors.lastname: Rios
authors.affiliation: Kidney Research Centre, Department of Medicine, Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada.|Institute of Cardiovascular and Medical Sciences, British Heart Foundation Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, UK.
authors.forename: Rhian M
authors.initials: RM
authors.lastname: Touyz
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology*; D051381:Rats
keywords: Large vessels; Primary culture; Small vessels; Vascular smooth muscle cells