title: Epstein-Barr Virus-induced Gene 2 Mediates Allergen-induced Leukocyte Migration into Airways.
abstract: Leukocyte recruitment to sites of allergic inflammation depends on the local production of priming cytokines, chemokines, and potentially other mediators. Previously, we showed that eosinophils (Eos) express numerous orphan G-protein-coupled receptors, including Epstein-Barr virus-induced gene 2 (EBI2). Despite its contribution to inflammatory diseases, the role of EBI2 in pulmonary eosinophilia is unknown.
  
  
  
  To determine whether oxysterol ligands for EBI2 are increased in asthma exacerbation, and if or how they promote Eos pulmonary migration.
  
  
  
  EBI2 ligands and pulmonary eosinophilia were measured in the bronchoalveolar lavage fluid from patients with mild asthma 48 hours after acute allergen challenge. In vitro, the ability of EBI2 ligands alone or in combination with IL-5 priming to induce the migration of human blood Eos was assessed.
  
  
  
  EBI2 was constitutively and stably expressed in peripheral blood Eos. Eos treated with the EBI2 ligands showed significantly increased transwell migration that was enhanced by priming with physiologic doses of IL-5. Migration was suppressed by inhibitors of the prolyl isomerase Pin1 or extracellular signal-regulated kinases (ERK) 1/2 or by pertussis toxin. EBI2 signaling activated Pin1 isomerase activity through a cascade that was sensitive to ERK inhibitors and pertussis toxin. The concentration of EBI2 ligands was significantly increased in the bronchoalveolar lavage fluid 48 hours after segmental allergen challenge and strongly correlated with the increased numbers of Eos, lymphocytes, and neutrophils in the airways.
  
  
  
  Oxysterols are increased in inflamed airways after allergen challenge and, through G-protein subunit α, ERK, and Pin1 signaling, likely participate in the regulation of Eos migration into the lung in people with asthma.
authors.affiliation: 1 Department of Pathology and.
authors.forename: Zhong-Jian
authors.initials: ZJ
authors.lastname: Shen
authors.affiliation: 1 Department of Pathology and.
authors.forename: Jie
authors.initials: J
authors.lastname: Hu
authors.affiliation: 1 Department of Pathology and.
authors.forename: Venkatesh P
authors.initials: VP
authors.lastname: Kashi
authors.affiliation: 2 Department of Medicine, Allergy, Pulmonary, and Critical Care Medicine Division, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Kelly
authors.affiliation: 2 Department of Medicine, Allergy, Pulmonary, and Critical Care Medicine Division, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin.
authors.forename: Loren C
authors.initials: LC
authors.lastname: Denlinger
authors.affiliation: 1 Department of Pathology and.
authors.forename: Kevan
authors.initials: K
authors.lastname: Lutchman
authors.affiliation: 3 Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas; and.
authors.forename: Jeffrey G
authors.initials: JG
authors.lastname: McDonald
authors.affiliation: 2 Department of Medicine, Allergy, Pulmonary, and Critical Care Medicine Division, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin.
authors.forename: Nizar N
authors.initials: NN
authors.lastname: Jarjour
authors.affiliation: 1 Department of Pathology and.
authors.forename: James S
authors.initials: JS
authors.lastname: Malter
mesh_terms: D000485:Allergens / Q000276:immunology*; D001249:Asthma / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D002465:Cell Movement; D004804:Eosinophils / Q000276:immunology; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes / Q000276:immunology*; D008168:Lung / Q000276:immunology*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000276:immunology*; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology
chemical_list: D000485:Allergens; C080105:GPR183 protein, human; D043562:Receptors, G-Protein-Coupled
keywords: EBI2; Pin1; asthma; eosinophils; migration