title: Structure and bioactivities of a polysaccharide isolated from Ganoderma lucidum in submerged fermentation.
abstract: In this study, a Ganoderma lucidum polysaccharide GLP-1-1 was isolated from a culture broth with Mw of 22014 Da. Monosaccharide contained glucose, mannose, and galactose with mole percentages of 92.33%, 7.55%, and 0.22%, respectively. Moreover, FTIR and methylation analysis were conducted to characterize the structural properties of GLP-1-1. The results of antioxidant activity analysis showed that GLP-1-1 had a great DPPH and ABTS radical scavenging activity. Meanwhile, GLP-1-1 also exhibited anti-tumor activity to A431 and MDA-MB-231 cells, and inhibitory rates were dose-dependent. During culturing with GLP-1-1, the G1/G0 cell percentage of A431 cells was increased from 48.64% to 84.52%, and the G1/G0 cell percentage of MDA-MB-231 cells was increased from 57.14% to 73.48%. Therefore, the anti-tumor activity of GLP-1-1 may be caused by inducing the G1/G0 arrest of tumor cells.
authors.affiliation: a National Engineering Laboratory for Cereal Fermentation Technology , Jiangnan University , Wuxi , China.|b School of Food Science and Technology , Jiangnan University , Wuxi, Jiangsu , China.|c National Engineering Research Center of Chinese Rice Wine , Shaoxing, Zhejiang , China.
authors.forename: Aisikaer
authors.initials: A
authors.lastname: Ai-Lati
authors.affiliation: a National Engineering Laboratory for Cereal Fermentation Technology , Jiangnan University , Wuxi , China.|b School of Food Science and Technology , Jiangnan University , Wuxi, Jiangsu , China.|c National Engineering Research Center of Chinese Rice Wine , Shaoxing, Zhejiang , China.
authors.forename: Shuangping
authors.initials: S
authors.lastname: Liu
authors.affiliation: a National Engineering Laboratory for Cereal Fermentation Technology , Jiangnan University , Wuxi , China.|b School of Food Science and Technology , Jiangnan University , Wuxi, Jiangsu , China.|c National Engineering Research Center of Chinese Rice Wine , Shaoxing, Zhejiang , China.
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Ji
authors.affiliation: b School of Food Science and Technology , Jiangnan University , Wuxi, Jiangsu , China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: a National Engineering Laboratory for Cereal Fermentation Technology , Jiangnan University , Wuxi , China.|b School of Food Science and Technology , Jiangnan University , Wuxi, Jiangsu , China.|c National Engineering Research Center of Chinese Rice Wine , Shaoxing, Zhejiang , China.
authors.forename: Jian
authors.initials: J
authors.lastname: Mao
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D019149:Bioreactors / Q000382:microbiology*; D045744:Cell Line, Tumor; D005285:Fermentation; D006801:Humans; D008070:Lipopolysaccharides / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism*; D008970:Molecular Weight; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology; D020940:Reishi / Q000254:growth & development / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D000970:Antineoplastic Agents; D008070:Lipopolysaccharides
keywords: EPS; Ganoderma lucidum; anti-tumor; antioxidant; structural properties