title: Analysis of Pseudomonas aeruginosa PAO1 Biofilm Protein Profile After Exposure to n-Butanolic Cyclamen coum Extract Alone and in Combination with Ciprofloxacin.
abstract: Pseudomonas aeruginosa biofilm-related infections are the major cause of premature death in cystic fibrosis patients. Strategies to induce biofilm dispersal are of interest, because of their potential in preventing biofilm-related infections. Our previous work demonstrated that n-butanolic Cyclamen coum extract with ciprofloxacin could eliminate 1- and 3-day-old P. aeruginosa PAO1 biofilms. To gain new insights into the role of C. coum extract and its synergistic effect with ciprofloxacin in eliminating P. aeruginosa PAO1 biofilms, two-dimensional gel electrophoresis (2-DE) in combination with mass spectrometry-based protein identification were used. Changes in the bacterial protein expression were analyzed when 3-day-old biofilm cells were exposed to the C. coum extract alone and in combination with ciprofloxacin. Proteins involved in alginate biosynthesis, quorum sensing, adaptation/protection, carbohydrate and amino acid metabolism showed a weaker expression in the C. coum extract-ciprofloxacin-treated biofilm cells compared to those in the untreated cells. Interestingly, the proteome of C. coum extract-ciprofloxacin-treated biofilm revealed more resemblance to the planktonic phenotype than to the biofilm phenotype. It appears that saponin extract in combination with ciprofloxacin causes biofilm disruption due to several mechanisms such as motility induction, cell envelope integrity perturbation, stress protein expression reduction, and more importantly, signal transduction perturbation. In conclusion, exposure to a combination of biofilm dispersal such as saponin extract and antimicrobial agents may offer a novel strategy to control preestablished, persistent P. aeruginosa biofilms and biofilm-related infections.
authors.affiliation: Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.|Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Morvarid
authors.initials: M
authors.lastname: Shafiei
authors.affiliation: Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.
authors.forename: Ahya
authors.initials: A
authors.lastname: Abdi-Ali
authors.affiliation: Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Shahcheraghi
authors.affiliation: Facility for Electron Microscopy Research, McGill University, 3640 Street, Montreal, Canada.
authors.forename: Hojatollah
authors.initials: H
authors.lastname: Vali
authors.affiliation: Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14155-6343, Tehran, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Shahbani Zahiri
authors.affiliation: Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14155-6343, Tehran, Iran. Akbari@nigeb.ac.ir.
authors.forename: Kambiz
authors.initials: K
authors.lastname: Akbari Noghabi
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects; D000596:Amino Acids / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D018441:Biofilms / Q000187:drug effects; D018533:Biomass; D000440:Butanols / Q000737:chemistry; D002244:Carbon / Q000378:metabolism; D002939:Ciprofloxacin / Q000494:pharmacology*; D031824:Cyclamen / Q000737:chemistry* / Q000378:metabolism; D004357:Drug Synergism; D005227:Fatty Acids / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000378:metabolism* / Q000502:physiology
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D000440:Butanols; D005227:Fatty Acids; D008070:Lipopolysaccharides; D010743:Phospholipids; D010936:Plant Extracts; D002939:Ciprofloxacin; D002244:Carbon
keywords: Biofilm; Ciprofloxacin; Cyclamen coum; Pseudomonas aeruginosa PAO1; Two-dimensional gel electrophoresis (2-DE)