title: Biocatalytic Total Synthesis of Ikarugamycin.
abstract: Nature provides an inexhaustible diversity of small organic molecules with beautiful molecular architectures that have strong and selective inhibitory activities. However, this tremendous biomedical potential often remains inaccessible, as the structural complexity of natural products can render their synthetic preparation extremely challenging. This problem is addressable by harnessing the biocatalytic procedures evolved by nature. In this work, we present an enzymatic total synthesis of ikarugamycin. The use of an iterative PKS/NRPS machinery and two reductases has allowed the construction of 15 carbon-carbon and 2 carbon-nitrogen bonds in a biocatalytic one-pot reaction. By scaling-up this method we demonstrate the applicability of biocatalytic approaches for the ex vivo synthesis of complex natural products.
authors.affiliation: Biosystems Chemistry, Department of Chemistry and Center for Integrated Protein Science Munich (CIPSM), Lichtenbergstrasse 4, 85748, Garching, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Greunke
authors.affiliation: Biosystems Chemistry, Department of Chemistry and Center for Integrated Protein Science Munich (CIPSM), Lichtenbergstrasse 4, 85748, Garching, Germany.
authors.forename: Anna
authors.initials: A
authors.lastname: Glöckle
authors.affiliation: Biosystems Chemistry, Department of Chemistry and Center for Integrated Protein Science Munich (CIPSM), Lichtenbergstrasse 4, 85748, Garching, Germany.
authors.forename: Janine
authors.initials: J
authors.lastname: Antosch
authors.affiliation: Biosystems Chemistry, Department of Chemistry and Center for Integrated Protein Science Munich (CIPSM), Lichtenbergstrasse 4, 85748, Garching, Germany.
authors.forename: Tobias A M
authors.identifier: 0000-0001-6013-3161
authors.initials: TA
authors.lastname: Gulder
keywords: biocatalysis; enzyme catalysis; ikarugamycin; natural products; total synthesis