title: Vein Interposition Model: A Suitable Model to Study Bypass Graft Patency.
abstract: Bypass grafting is an established treatment method for coronary artery disease. Graft patency continues to be the Achilles heel of saphenous vein grafts. Research models for bypass graft failure are essential for a better understanding of pathobiological and pathophysiological processes during graft patency loss. Large animal models, such as pigs or sheep, resemble human anatomical structures but require special facilities and equipment. This video describes a rat vein interposition model to investigate vein graft patency loss. Rats are inexpensive and easy to handle. Compared to mouse models, the convenient size of rats permits better operability and enables a sufficient amount of material to be obtained for further diverse analysis. In brief, the inferior epigastric vein of a donor rat is harvested and used to replace a segment of the femoral artery. Anastomosis is conducted via single stitches and sealed with fibrin glue. Graft patency can be monitored non-invasively using duplex sonography. Myointimal hyperplasia, which is the main cause for graft patency loss, develops progressively over time and can be calculated from histological cross sections.
authors.affiliation: Transplant and Stem Cell Immunobiology Lab, University Heart Center Hamburg; Department of Surgery, Transplant and Stem Cell Immunobiology Lab, University of California San Francisco (UCSF); Cardiovascular Research Center (CVRC) and DZHK German Center for Cardiovascular Research), partner site Hamburg/Kiel/Luebeck; Cardiovascular Surgery, University Heart Center Hamburg.
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.affiliation: Transplant and Stem Cell Immunobiology Lab, University Heart Center Hamburg; Department of Surgery, Transplant and Stem Cell Immunobiology Lab, University of California San Francisco (UCSF); Cardiovascular Research Center (CVRC) and DZHK German Center for Cardiovascular Research), partner site Hamburg/Kiel/Luebeck.
authors.forename: Grigol
authors.initials: G
authors.lastname: Tediashvili
authors.affiliation: Cardiovascular Surgery, University Heart Center Hamburg.
authors.forename: Simon
authors.initials: S
authors.lastname: Pecha
authors.affiliation: Cardiovascular Surgery, University Heart Center Hamburg.
authors.forename: Hermann
authors.initials: H
authors.lastname: Reichenspurner
authors.affiliation: Transplant and Stem Cell Immunobiology Lab, University Heart Center Hamburg; Department of Surgery, Transplant and Stem Cell Immunobiology Lab, University of California San Francisco (UCSF); Cardiovascular Research Center (CVRC) and DZHK German Center for Cardiovascular Research), partner site Hamburg/Kiel/Luebeck; Cardiovascular Surgery, University Heart Center Hamburg.
authors.forename: Tobias
authors.initials: T
authors.lastname: Deuse
authors.affiliation: Transplant and Stem Cell Immunobiology Lab, University Heart Center Hamburg; Department of Surgery, Transplant and Stem Cell Immunobiology Lab, University of California San Francisco (UCSF); Cardiovascular Research Center (CVRC) and DZHK German Center for Cardiovascular Research), partner site Hamburg/Kiel/Luebeck; Cardiovascular Surgery, University Heart Center Hamburg; Sonja.Schrepfer@ucsf.edu.
authors.forename: Sonja
authors.initials: S
authors.lastname: Schrepfer
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D003324:Coronary Artery Disease / Q000601:surgery*; D005263:Femoral Artery / Q000601:surgery; D023421:Models, Animal; D051381:Rats; D012501:Saphenous Vein / Q000601:surgery; D058017:Vascular Grafting / Q000379:methods*; D014654:Vascular Patency