title: Wet in situ transesterification of microalgae using ethyl acetate as a co-solvent and reactant.
abstract: This study addresses wet in situ transesterification of microalgae for the production of biodiesel by introducing ethyl acetate as both reactant and co-solvent. Ethyl acetate and acid catalyst are mixed with wet microalgae in one pot and the mixture is heated for simultaneous lipid extraction and transesterification. As a single reactant and co-solvent, ethyl acetate can provide higher FAEE yield and more saccharification of carbohydrates than the case of binary ethanol and chloroform as a reactant and a co-solvent. The optimal yield was 97.8wt% at 114°C and 4.06M catalyst with 6.67mlEtOAC/g dried algae based on experimental results and response surface methodology (RSM). This wet in situ transesterification of microalgae using ethyl acetate doesn't require an additional co-solvent and it also promises more economic benefit as combining extraction and transesterification in a single process.
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Jeongseok
authors.initials: J
authors.lastname: Park
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Bora
authors.initials: B
authors.lastname: Kim
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea; ABC Biomass R&D Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Yong Keun
authors.initials: YK
authors.lastname: Chang
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea. Electronic address: jaewlee@kaist.ac.kr.
authors.forename: Jae W
authors.initials: JW
authors.lastname: Lee
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D019342:Acetic Acid / Q000032:analysis; D000143:Acids / Q000494:pharmacology; D000704:Analysis of Variance; D056804:Biofuels; D002384:Catalysis; D002725:Chloroform / Q000494:pharmacology; D004951:Esterification / Q000187:drug effects; D004952:Esters / Q000032:analysis; D000431:Ethanol / Q000494:pharmacology; D006813:Humidity; D058086:Microalgae / Q000187:drug effects / Q000378:metabolism*; D012997:Solvents / Q000494:pharmacology*
chemical_list: D000085:Acetates; D000143:Acids; D056804:Biofuels; D004952:Esters; D012997:Solvents; D000431:Ethanol; C007650:ethyl acetate; D002725:Chloroform; D019342:Acetic Acid
keywords: Biodiesel; Ethyl acetate; Microalgae; Response surface methodology; Wet in situ transesterification