title: Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes.
abstract: Semiconducting single-wall carbon nanotubes (SWNTs) are a class of optically active nanomaterial that fluoresce in the near infrared, coinciding with the optical window where biological samples are most transparent. Here, we outline techniques to adsorb amphiphilic polymers and polynucleic acids onto the surface of SWNTs to engineer their corona phases and create novel molecular sensors for small molecules and proteins. These functionalized SWNT sensors are both biocompatible and stable. Polymers are adsorbed onto the nanotube surface either by direct sonication of SWNTs and polymer or by suspending SWNTs using a surfactant followed by dialysis with polymer. The fluorescence emission, stability, and response of these sensors to target analytes are confirmed using absorbance and near-infrared fluorescence spectroscopy. Furthermore, we demonstrate surface immobilization of the sensors onto glass slides to enable single-molecule fluorescence microscopy to characterize polymer adsorption and analyte binding kinetics.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California Berkeley.
authors.forename: Jackson T
authors.initials: JT
authors.lastname: Del Bonis-O'Donnell
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California Berkeley.
authors.forename: Abraham
authors.initials: A
authors.lastname: Beyene
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California Berkeley.
authors.forename: Linda
authors.initials: L
authors.lastname: Chio
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California Berkeley.
authors.forename: Gözde
authors.initials: G
authors.lastname: Demirer
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California Berkeley.
authors.forename: Darwin
authors.initials: D
authors.lastname: Yang
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California Berkeley; California Institute for Quantitative Biosciences (QB3), University of California Berkeley; landry@berkeley.edu.
authors.forename: Markita P
authors.initials: MP
authors.lastname: Landry
mesh_terms: D000327:Adsorption; D040761:Biomimetic Materials / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013010:Sonication; D019265:Spectroscopy, Near-Infrared; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D037742:Nanotubes, Carbon; D011108:Polymers; D013501:Surface-Active Agents