title: Fatty acid composition and mechanisms of the protective effects of myrtle berry seed aqueous extract in alcohol-induced peptic ulcer in rat.
abstract: This study aimed to investigate the antiulcer and antioxidant activities of myrtle berry seed aqueous extract (MBSAE) in a peptic ulcer model induced by ethanol in male Wistar rats. MBSAE is rich in total polyphenols, total flavonoids, and unsaturated fatty acids, particularly linoleic (18:2) and oleic (18:1) acids. MBSAE also exhibited in vitro antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50= 172.1 μg/mL) and superoxide anion (IC50= 200.24 μg/mL) scavenging activities. In vivo, MBSAE provided dose-dependent protection against ethanol-induced gastric and duodenal macroscopic and histological alterations. Also, it inhibited secretory profile disturbances and lipid peroxidation, and preserved normal antioxidant enzyme activities and nonenzymatic antioxidant levels. More importantly, we showed that acute alcohol intoxication increased gastric and duodenal calcium, hydrogen peroxide, and free iron levels, whereas MBSAE treatment protected against intracellular mediator deregulation. In conclusion, we suggest that MBSAE has potent protective effects against alcohol-induced peptic ulcer in rat. This protection might be related in part to its antioxidant properties as well as its opposite effects on some studied intracellular mediators.
authors.affiliation: a Laboratoire de Physiologie Intégrée, Faculté des Sciences de Bizerte, 7021 Zarzouna, Université de Carthage, Tunisia.|b Laboratoire de Physiologie Fonctionnelle et Valorisation des Bio-Ressources - Institut Supérieur de Biotechnologie de Béja, Université de Jendouba, Avenue Habib Bourguiba - B.P. 382 - 9000 Béja, Tunisia.
authors.forename: Mohamed-Amine
authors.initials: MA
authors.lastname: Jabri
authors.affiliation: b Laboratoire de Physiologie Fonctionnelle et Valorisation des Bio-Ressources - Institut Supérieur de Biotechnologie de Béja, Université de Jendouba, Avenue Habib Bourguiba - B.P. 382 - 9000 Béja, Tunisia.
authors.forename: Kais
authors.initials: K
authors.lastname: Rtibi
authors.affiliation: c Laboratoire d'anatomie pathologique humaine et expérimentale, Institut Pasteur de Tunis, 13, Place Pasteur, Tunis 1002, BP-74, Tunisia.
authors.forename: Haifa
authors.initials: H
authors.lastname: Tounsi
authors.affiliation: d Laboratoire des substances naturelles, Institut National de Recherche et d'Analyse Physico-chimique, Sidi Thabet, 2020 Ariana, Tunisia.
authors.forename: Karim
authors.initials: K
authors.lastname: Hosni
authors.affiliation: b Laboratoire de Physiologie Fonctionnelle et Valorisation des Bio-Ressources - Institut Supérieur de Biotechnologie de Béja, Université de Jendouba, Avenue Habib Bourguiba - B.P. 382 - 9000 Béja, Tunisia.
authors.forename: Lamjed
authors.initials: L
authors.lastname: Marzouki
authors.affiliation: a Laboratoire de Physiologie Intégrée, Faculté des Sciences de Bizerte, 7021 Zarzouna, Université de Carthage, Tunisia.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Sakly
authors.affiliation: a Laboratoire de Physiologie Intégrée, Faculté des Sciences de Bizerte, 7021 Zarzouna, Université de Carthage, Tunisia.|b Laboratoire de Physiologie Fonctionnelle et Valorisation des Bio-Ressources - Institut Supérieur de Biotechnologie de Béja, Université de Jendouba, Avenue Habib Bourguiba - B.P. 382 - 9000 Béja, Tunisia.
authors.forename: Hichem
authors.initials: H
authors.lastname: Sebai
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D019610:Cytoprotection / Q000187:drug effects; D004386:Duodenum / Q000187:drug effects / Q000473:pathology; D000431:Ethanol / Q000009:adverse effects*; D005227:Fatty Acids / Q000032:analysis*; D005419:Flavonoids / Q000032:analysis; D006861:Hydrogen Peroxide / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D031651:Myrtus / Q000737:chemistry*; D010437:Peptic Ulcer / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D059808:Polyphenols / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry*; D013270:Stomach / Q000187:drug effects / Q000473:pathology; D014867:Water / Q000737:chemistry
chemical_list: D005227:Fatty Acids; D005419:Flavonoids; D010936:Plant Extracts; D059808:Polyphenols; D014867:Water; D000431:Ethanol; D006861:Hydrogen Peroxide; D007501:Iron; D002118:Calcium
keywords: acides gras insaturés; acidité libre; antioxidant; antioxydant; baies de myrte; free acidity; myrtle berries; peptic ulcer; ulcère peptique; unsaturated fatty acids