title: Expression of Biomarkers CXCR4, IL11-RA, TFF1, MLF1P in Advanced Breast Cancer Patients with Bone Metastatic: a Diagnostic Study.
abstract: OBJECTIVE
  to analyze expression of biomarkers CXCR4, IL11-RA, TFF1 and MLF1P, and clinicopathology in advanced breast cancer patients with bone metastatic.
  
  
  METHODS
  this is a cross-sectional study. Analysis was done against a total of 92 breast cancer patients, including 46 bone metastatic patients and 46 non-bone metastatic patients. Immunohistochemistry and microarray analysis was performed in 81 formalin fixed paraffin embedded (FFPE) samples from 81 patients were used. Data were collected through medical records, immunohistochemistry (IHC), and microarray with nanoString nCounterTM.
  
  
  RESULTS
  this article is part one of a two stage reporting research results. In part one we got the results of the IHC analysis, IL11-RA with cut-off ≥103.5 showed OR 3.803 (95 % confidence interval [CI], 1.375-10.581), p=0.010, MLF1P with cut-off ≥83.0 OR 2.784 (95% CI, 1.009-7.681), p=0.048, and ER+ OR 7.640 (95 % CI, 2.599-22.459), p<0.000, were associated with bone metastastic incidences in advanced breast cancer, and were statistically significantly different. A combination of IL-11RA, MLF1P and ER+, showed an accuracy of approaching 80% to discriminate between bone metastatic and non bone metastatic in advanced breast cancer patients.
  
  
  CONCLUSIONS
  IL11-RA, MLF1P, and ER+ were the determinants that were associated with increasing bone metastasis incidence.
authors.affiliation: Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - Cipto Mangunkusumo Hospital, Jakarta, Indonesia. cosphiadi@gmail.com.
authors.forename: Cosphiadi
authors.initials: C
authors.lastname: Irawan
authors.forename: Djumhana
authors.initials: D
authors.lastname: Atmakusumah
authors.forename: Nurjati Chaeranie
authors.initials: NC
authors.lastname: Siregar
authors.forename: The Bin
authors.initials: TB
authors.lastname: Tean
authors.forename: Lim Weng
authors.initials: LW
authors.lastname: Kong
authors.forename: Ong Choon
authors.initials: OC
authors.lastname: Kiat
authors.forename: Abdulmuthalib
authors.initials: A
authors.lastname: Abdulmuthalib
authors.forename: Alida
authors.initials: A
authors.lastname: Harahap
authors.forename: Muchtaruddin
authors.initials: M
authors.lastname: Mansyur
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001859:Bone Neoplasms / Q000556:secondary*; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053703:Interleukin-11 Receptor alpha Subunit / Q000378:metabolism; D046228:Microarray Analysis; D008875:Middle Aged; D009687:Nuclear Proteins / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000378:metabolism; D000071163:Trefoil Factor-1 / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; C527276:CXCR4 protein, human; C508428:IL11RA protein, human; D053703:Interleukin-11 Receptor alpha Subunit; C100853:MLF2 protein, human; D009687:Nuclear Proteins; D019718:Receptors, CXCR4; C051838:TFF1 protein, human; D000071163:Trefoil Factor-1
keywords: ER+; ESR1; IL11-RA; MLF1P; breast cancer; metastatic bone disease