title: Selenoprotein K modulate intracellular free Ca2+by regulating expression of calcium homoeostasis endoplasmic reticulum protein.
abstract: Selenoprotein K (SelK) is an 11-kDa selenoprotein, which may be involved in the regulation of oxidative stress, endoplasmic reticulum (ER) stress and immune response. To explore the function of SelK in the process of immune response, several short-hairpin RNAs (shRNA) were designed for the construction of recombinant plasmids to down-regulate the expression of SelK gene in vitro. These shRNAs specifically and efficiently interfered with the expression of SelK at both mRNA and protein levels. The expression of calcium homoeostasis endoplasmic reticulum protein (CHERP) and the intracellular free Ca2+concentration were significantly down-regulated in anti-CD3 stimulated SelK-knockdown cells. The expression of Interleukin 2 receptor alpha chain (IL-2Rα) and the secretion of Interleukin 4 (IL-4), which play a significant role in the process of T cell activation and proliferation, were also reduced in SelK-knockdown cells. Selenomethionine (Se-Met) at an optimum concentration of 5 μM could up-regulate SelK expression and reverse the change of the expression of CHERP and the intracellular free calcium caused by SelK-knockdown. These results hereby imply SelK may regulate the release of Ca2+by CHERP and play an important role in the proliferation and differentiation of T cell by TCR stimulation.
authors.affiliation: Shenzhen Center for Disease Control and Prevention, 518055, Shenzhen, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Shenzhen Center for Disease Control and Prevention, 518055, Shenzhen, China.
authors.forename: Ruimin
authors.initials: R
authors.lastname: Li
authors.affiliation: Shenzhen Center for Disease Control and Prevention, 518055, Shenzhen, China.
authors.forename: Yalan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Shenzhen Center for Disease Control and Prevention, 518055, Shenzhen, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Wang
authors.affiliation: Shenzhen Center for Disease Control and Prevention, 518055, Shenzhen, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: Shenzhen Center for Disease Control and Prevention, 518055, Shenzhen, China.
authors.forename: Dana
authors.initials: D
authors.lastname: Huang
authors.affiliation: Shenzhen Center for Disease Control and Prevention, 518055, Shenzhen, China.
authors.forename: Chunli
authors.initials: C
authors.lastname: Wu
authors.affiliation: Shenzhen Center for Disease Control and Prevention, 518055, Shenzhen, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.affiliation: Shenzhen Center for Disease Control and Prevention, 518055, Shenzhen, China.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Shenzhen Center for Disease Control and Prevention, 518055, Shenzhen, China. Electronic address: renlizhangszcdc@163.com.
authors.forename: Renli
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Shenzhen Center for Disease Control and Prevention, 518055, Shenzhen, China. Electronic address: cjinquan@szcdc.net.
authors.forename: Jinquan
authors.initials: J
authors.lastname: Cheng
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000502:physiology; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D007424:Intracellular Fluid / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D016601:RNA-Binding Proteins / Q000378:metabolism*; D051140:Selenoproteins / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: C410225:CHERP protein, human; D004268:DNA-Binding Proteins; D008565:Membrane Proteins; D016601:RNA-Binding Proteins; D051140:Selenoproteins; C000592898:selenoprotein K, human; D002118:Calcium
keywords: CHERP; Ca(2+); IL-2Rα; IL-4; SelK