title: The Effect of Autologous Platelet-Rich Plasma on Bone Regeneration by Autologous Mesenchymal Stem Cells Loaded onto Allogeneic Cancellous Bone Granules.
abstract: To develop a clinically effective bone regeneration strategy, we compared bone regeneration using allogeneic cancellous bone granule scaffolds loaded with autologous bone marrow-derived mesenchymal stem cells (BM-MSC) with or without autologous platelet-rich plasma (PRP). Critical-sized segmental bone defects were made at the mid-shaft of both radiuses in 41 New Zealand White rabbits. Small-sized allogeneic cancellous bone granules (300-700 μm in diameter) loaded with BM-MSC were implanted on one side, and PRP was added. On the other side, autologous BM-MSC loaded onto allogeneic cancellous granules were grafted as a control. Bone regeneration was assessed by radiographic evaluation at 4, 8, and 16 weeks postimplantation and by micro-computed tomography (micro-CT) and histological evaluation of the retrieved specimens at 8 and 16 weeks. The experimental group did not show significantly higher bone quantity indices than the control group at any time point. Micro-CT analysis revealed that both groups had similar mean total volumes, surface areas, and other parameters at 8 and 16 weeks. Histological evaluation of 8- and 16-week specimens also showed a similar progression of new bone formation and maturation. In this experiment using a contralateral control group in the same individual, an initial single addition of PRP in allogeneic cancellous bone granules loaded with BM-MSC for critical-sized bone defects in the weight-bearing area did not induce a consequent difference in bone healing. Further research into the optimal preparation and application of PRP is necessary. Furthermore, studies involving a greater number of subjects and larger experimental animals could determine the clinical relevance of PRP treatment.
authors.affiliation: Department of Orthopedic Surgery, St. Vincent's Hospital, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Min Wook
authors.initials: MW
authors.lastname: Joo
authors.forename: Seung Jae
authors.initials: SJ
authors.lastname: Chung
authors.forename: Seung Han
authors.initials: SH
authors.lastname: Shin
authors.forename: Yang-Guk
authors.initials: YG
authors.lastname: Chung
mesh_terms: D000818:Animals; D015519:Bone Density; D001861:Bone Regeneration; D000071556:Cancellous Bone / Q000166:cytology / Q000000981:diagnostic imaging / Q000502:physiology* / Q000648:ultrastructure; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D011817:Rabbits; D016474:Weight-Bearing; D055114:X-Ray Microtomography
keywords: Allografts; Bone regeneration; Bone transplantation; Mesenchymal stem cell transplantation; Platelet-rich plasma; Tissue engineering