title: Species Identification and Molecular Typing ofLeishmaniaSpp. Using Targeting HSP70 Gene in Suspected Patients of Cutaneous Leishmaniasis from Sistan and Baluchestan Province, Southeast Iran.
abstract: BACKGROUND
  Leishmaniasis is a sand fly-borne disease caused by the protozoan parasites belonging to the genusLeishmania. Because of the preventing and controlling methods, clinical course, prognosis and choice of treatment are differing from species; differentiation of species is critical. The present study was aimed to detect the parasite species using the PCR-RFLP method.
  
  
  METHODS
  A total of 130 Giemsa-Stained slides from suspected Cutaneous leishmaniasis (CL) patients were examined under a light microscope at ×1000. DNA from each slide was extracted PCR method was undertaken with HSP70 genes and the PCR products were digested with a restriction enzyme HaeIII (BsuR1). The study was conducted in the laboratory of Zahedan University of Medical Sciences in the Sistan and Baluchestan Province, southeastern Iran in 2015.
  
  
  RESULTS
  From 130 suspected samples, 59 (45.3%) were positive by the microscopic examination, meanwhile 64 (49.2%) were positive by PCR-RFLP,Leishmaniaspecies were recognized, andL. tropicawas introduced as predominant species in current study.
  
  
  CONCLUSIONS
  PCR-RFLP is a valuable technique for distinguish ofLeishmaniaspecies. Furthermore, anthroponotic CL is the dominant cause of CL in Sistan and Baluchestan Province.
authors.affiliation: Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, Iran; Dept. of Parasitology, Mycology and Entomology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
authors.forename: Hadi
authors.initials: H
authors.lastname: Mirahmadi
authors.affiliation: Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, Iran; Dept. of Parasitology, Mycology and Entomology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Salimi Khorashad
authors.affiliation: Dept. of Parasitology, Mycology and Entomology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Sohrabnahad
authors.affiliation: Dept. of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Peyman
authors.initials: P
authors.lastname: Heydarian
authors.affiliation: Dept. of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Negar
authors.initials: N
authors.lastname: Bizhani
keywords: HSP70 gene; Human; Iran; Leishmania; PCR-RFLP