title: Effects of Different Simple Triglycerides on Cell Fatty Acid Compositions, Proliferation-Related Protein, and Gene Expressions Induced by Oxidized-LDL in HUVSMCs.
abstract: The proliferating effects of 3 different simple triglycerides (tristearin, triolein, and trilinolein) on the human umbilical vein smooth muscle cells (HUVSMCs) induced by oxidized-LDL (ox-LDL) were investigated in this study. The protein and mRNA gene expressions of proliferating cell nuclear antigen (PCNA), smooth muscle-α-actin (SM-α-actin), and monocyte chemoattractant protein-1 (MCP-1) in HUVSMCs were measured by Western blotting and real-time quantitative polymerase chain reaction (PCR). It was shown that in tristearin (SSS) treated HUVSMCs, the saturated fatty acid content was increased, and the compositions of polyunsaturated fatty acid (PUFA) and monounsaturated fatty acid were decreased significantly. On the other hand, triolein (OOO) and trilinolein (LLL) significantly increased the levels of some typical PUFA such as arachidonic acid, eicosapentaenoic acid, and docosahexaenoic acid. Moreover, LLL and OOO could upregulate the protein and mRNA expressions of PCNA, MCP-1 as well as downregulate the expression of SM-α-actin. The results also showed that, SSS had significant promotion effects on the proliferation of HUVSMCs induced by ox-LDL. Although both LLL and OOO could inhibit the proliferation of HUVSMCs induced by ox-LDL, and might have certain inhibition of the atherosclerotic process.
authors.affiliation: State Key Laboratory of Food Science and Technology, Univ. of Nanchang, Nanchang, 330047, Jiangxi, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Food Science and Technology, Univ. of Nanchang, Nanchang, 330047, Jiangxi, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Food Science and Technology, Univ. of Nanchang, Nanchang, 330047, Jiangxi, China.
authors.forename: Kaiyun
authors.initials: K
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Food Science and Technology, Univ. of Nanchang, Nanchang, 330047, Jiangxi, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Food Science and Technology, Univ. of Nanchang, Nanchang, 330047, Jiangxi, China.
authors.forename: Yawei
authors.initials: Y
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Food Science and Technology, Univ. of Nanchang, Nanchang, 330047, Jiangxi, China.|Inst. for Advanced Study, Univ. of Nanchang, Nanchang, 330031, Jiangxi, China.
authors.forename: Zeyuan
authors.initials: Z
authors.lastname: Deng
mesh_terms: D000199:Actins / Q000378:metabolism; D050197:Atherosclerosis / Q000378:metabolism; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000737:chemistry; D015870:Gene Expression / Q000187:drug effects; D005786:Gene Expression Regulation; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D014280:Triglycerides / Q000737:chemistry*; D014304:Triolein / Q000737:chemistry
chemical_list: D000199:Actins; C111835:CCL2 protein, human; D018932:Chemokine CCL2; D005231:Fatty Acids, Unsaturated; D008077:Lipoproteins, LDL; D018809:Proliferating Cell Nuclear Antigen; D014280:Triglycerides; C099252:oxidized low density lipoprotein; D014304:Triolein; C022618:tristearin; C009564:trilinolein
keywords: HUVSMC; ox-LDL; trilinolein; triolein; tristearin