title: Online flow cytometry, an interesting investigation process for monitoring lipid accumulation, dimorphism, and cells' growth in the oleaginous yeastYarrowia lipolyticaJMY 775.
abstract: This study aims to understand and better control the main biological mechanisms and parameters modulating the various phenomena affectingYarrowia lipolyticaJMY775 and its lipids accumulation. The results obtained in this study stress forward that the use of an original tool, consisting of coupling bioreactors to online flow cytometry, is highly efficient. Throughout 48 h of culturing, this emerging process allowed an online continuous observation of the effects of pH and/or aeration on the cell growth and dimorphism and lipid accumulation byY. lipolytica. This present study showed clearly that online flow cytometry is an advantageous tool for the real-time monitoring of microbial culture at a single-cell level. Indeed, the present investigation showed for the first time that profiling of the various phenomena and their monitoring upon culture time is now possible by coupling online cytometry with culture bioreactors.
authors.affiliation: Unité de Bio-Industries, Passage des déportés, Université de Liège/Gembloux AGRO-BIO Tech, 2-5030 Gembloux, Belgium ; INATAA, Université Frères Mentouri Constantine, Route de Aïn El Bey, 25000 Constantine, Algeria ; Unité de science des aliments et formulation Passage des déportés, Université de Liège/Gembloux AGRO-BIO Tech, 2-5030 Gembloux, Belgium.
authors.forename: Doria Naila
authors.initials: DN
authors.lastname: Bouchedja
authors.affiliation: Unité de science des aliments et formulation Passage des déportés, Université de Liège/Gembloux AGRO-BIO Tech, 2-5030 Gembloux, Belgium.
authors.forename: Sabine
authors.initials: S
authors.lastname: Danthine
authors.affiliation: Unité de Bio-Industries, Passage des déportés, Université de Liège/Gembloux AGRO-BIO Tech, 2-5030 Gembloux, Belgium.
authors.forename: Tambi
authors.initials: T
authors.lastname: Kar
authors.affiliation: Unité de Bio-Industries, Passage des déportés, Université de Liège/Gembloux AGRO-BIO Tech, 2-5030 Gembloux, Belgium.
authors.forename: Patrick
authors.initials: P
authors.lastname: Fickers
authors.affiliation: INATAA, Université Frères Mentouri Constantine, Route de Aïn El Bey, 25000 Constantine, Algeria.
authors.forename: Abdelghani
authors.initials: A
authors.lastname: Boudjellal
authors.affiliation: Unité de Bio-Industries, Passage des déportés, Université de Liège/Gembloux AGRO-BIO Tech, 2-5030 Gembloux, Belgium.
authors.forename: Frank
authors.initials: F
authors.lastname: Delvigne
keywords: Cell growth; Cells’ shape; Lipid accumulation; Online flow cytometry; Yarrowia lipolytica