title: Assessment of Caveolae/Lipid Rafts in Isolated Cells.
abstract: This chapter outlines protocols to evaluate protein localization, recruitment or phosphorylation levels in cholesterol/sphingolipids-enriched cell membrane domains and recommends experimental designs with pharmacological tolls to evaluate potential cell functions associated with these domains. We emphasize the need for the combination of several approaches towards understanding the protein components and cellular functions attributed to these distinct microdomains.
authors.affiliation: Kidney Research Centre, Department of Medicine, Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada.
authors.forename: G E
authors.initials: GE
authors.lastname: Callera
authors.affiliation: Kidney Research Centre, Department of Medicine, Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada.|Department of Pharmacology, Medical School of Ribeirao Preto, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Thiago
authors.initials: T
authors.lastname: Bruder-Nascimento
authors.affiliation: Kidney Research Centre, Department of Medicine, Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada. rhian.Touyz@glasgow.ac.uk.|Institute of Cardiovascular & Medical Sciences, BHF Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, Scotland, UK. rhian.Touyz@glasgow.ac.uk.
authors.forename: R M
authors.initials: RM
authors.lastname: Touyz
mesh_terms: D000818:Animals; D002254:Carbonates / Q000378:metabolism; D021941:Caveolae / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002784:Cholesterol / Q000737:chemistry / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D021962:Membrane Microdomains / Q000737:chemistry / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000378:metabolism; D051381:Rats; D013107:Sphingolipids / Q000737:chemistry / Q000378:metabolism; D047392:beta-Cyclodextrins / Q000737:chemistry / Q000378:metabolism
chemical_list: D002254:Carbonates; D013107:Sphingolipids; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; C005686:sodium carbonate; D002784:Cholesterol
keywords: Caveolae; Detergent-free method; Lipid rafts; Methyl-β-cyclodextrin; Statins