title: Maternal short-chain fructo-oligosaccharide supplementation increases intestinal cytokine secretion, goblet cell number, butyrate concentration and Lawsonia intracellularis humoral vaccine response in weaned pigs.
abstract: Prebiotic supplementation modulates immune system development and function. However, less is known about the effects of maternal prebiotic consumption on offspring intestinal defences and immune system responsiveness. We investigated the effects of maternal short-chain fructo-oligosaccharide (scFOS) supplementation on mucin-secreting cells, ileal secretory IgA and cytokine secretion of weaned offspring and their humoral response to an oral vaccine against obligate intracellular Lawsonia intracellularis. Sows were fed a control diet (CTRL) or scFOS-supplemented diet during the last third of gestation and throughout lactation. At weaning, each litter was divided into two groups receiving a post-weaning CTRL or scFOS diet for a month. Pigs from the four groups were either non-vaccinated (n 16) or vaccinated (n 117) at day 33. Biomarkers related to intestinal defences and immune parameters were analysed 3 weeks later. SCFA production was assessed over time in suckling and weaned pigs. Maternal scFOS supplementation improved ileal cytokine secretions (interferon (IFN)-γ, P<0·05; IL-4, P=0·07) and tended to increase caecal goblet cell number (P=0·06). It increased IgA vaccine response in the serum (P<0·01) and ileal mucosa (P=0·08). Higher bacterial fermentative activity was observed during lactation (total faecal SCFA, P<0·001) and after weaning (colonic butyrate, P=0·10) in pigs from scFOS-supplemented mothers. No synergistic effect between maternal and post-weaning scFOS supplementation was observed. Therefore, maternal scFOS supplementation has long-lasting consequences by strengthening gut defences and immune response to a vaccine against an intestinal obligate intracellular pathogen. Prebiotic consumption by gestating and lactating mothers is decisive in modulating offspring intestinal immunity.
authors.affiliation: 1French National Institute for Agricultural Research (INRA),UR1341 Food and Digestive, Nervous and Behavioural Adaptations (ADNC),Saint-Gilles,F-35590,France.
authors.forename: Cindy
authors.initials: C
authors.lastname: Le Bourgot
authors.affiliation: 1French National Institute for Agricultural Research (INRA),UR1341 Food and Digestive, Nervous and Behavioural Adaptations (ADNC),Saint-Gilles,F-35590,France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Le Normand
authors.affiliation: 1French National Institute for Agricultural Research (INRA),UR1341 Food and Digestive, Nervous and Behavioural Adaptations (ADNC),Saint-Gilles,F-35590,France.
authors.forename: Michèle
authors.initials: M
authors.lastname: Formal
authors.affiliation: 2Tereos,Marckolsheim,F-67390,France.
authors.forename: Frédérique
authors.initials: F
authors.lastname: Respondek
authors.affiliation: 1French National Institute for Agricultural Research (INRA),UR1341 Food and Digestive, Nervous and Behavioural Adaptations (ADNC),Saint-Gilles,F-35590,France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Blat
authors.affiliation: 2Tereos,Marckolsheim,F-67390,France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Apper
authors.affiliation: 1French National Institute for Agricultural Research (INRA),UR1341 Food and Digestive, Nervous and Behavioural Adaptations (ADNC),Saint-Gilles,F-35590,France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Ferret-Bernard
authors.affiliation: 1French National Institute for Agricultural Research (INRA),UR1341 Food and Digestive, Nervous and Behavioural Adaptations (ADNC),Saint-Gilles,F-35590,France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Le Huërou-Luron
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D002087:Butyrates / Q000097:blood*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D045824:Desulfovibrionaceae Infections / Q000382:microbiology / Q000662:veterinary; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D005260:Female; D020397:Goblet Cells / Q000502:physiology*; D020639:Lawsonia Bacteria; D039382:Maternal Nutritional Physiological Phenomena; D009844:Oligosaccharides / Q000008:administration & dosage* / Q000737:chemistry; D056692:Prebiotics; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control
chemical_list: D001428:Bacterial Vaccines; D002087:Butyrates; D016207:Cytokines; D009844:Oligosaccharides; D056692:Prebiotics; C116580:fructooligosaccharide
keywords:  IFN interferon;  PND postnatal day;  sIgA secretory IgA;  scFOS short-chain fructo-oligosaccharides; Intestinal immune system; Maternal nutrition; Prebiotics; Short-chain fructo-oligosaccharides; Vaccination