title: Molecular epidemiological investigation of Brucella melitensis circulating in Mongolia by MLVA16.
abstract: Mongolia has a high incidence of brucellosis in human and animals due to livestock husbandry. To investigate the genetic characteristics of Mongolian B. melitensis, an MLVA (multi-locus variable-number tandem-repeat analysis)-16 assay was performed with 94 B. melitensis isolates. They were identified as B. melitensis biovar (bv.) 1 (67), 3 (10) and Rev. 1 vaccine strains (17) using a classical biotyping and multiplex PCR. In genotyping, three human isolates were grouped at 2 genotypes with sheep isolates, and it implies that B. melitensis are cross-infected between human and livestock. In the parsimony analysis, Mongolian B. melitensis isolates had high genetic similarity with Chinese strains, likely due to the geographical proximity, clustered distinctively as compared with other foreign isolates. B. melitensis Rev. 1 vaccine strains were divided into 4 genotypes with 92% similarity. In the analysis of Rev.1 strains, the risk of mutation of vaccine strain might not be overlooked. Animal quarantines should be strengthened to prevent the spread of Brucella species among adjacent countries.
authors.affiliation: OIE Reference Laboratory for Brucellosis, Bacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea.
authors.forename: Sung-Il
authors.initials: SI
authors.lastname: Kang
authors.affiliation: OIE Reference Laboratory for Brucellosis, Bacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea.
authors.forename: Moon
authors.initials: M
authors.lastname: Her
authors.affiliation: Institute of Veterinary Medicine, Zaisan, 17024, Ulaanbaatar, Mongolia.
authors.forename: Janchivdorj
authors.initials: J
authors.lastname: Erdenebaataar
authors.affiliation: Institute of Veterinary Medicine, Zaisan, 17024, Ulaanbaatar, Mongolia.
authors.forename: Batbaatar
authors.initials: B
authors.lastname: Vanaabaatar
authors.affiliation: OIE Reference Laboratory for Brucellosis, Bacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea.
authors.forename: Hyorim
authors.initials: H
authors.lastname: Cho
authors.affiliation: OIE Reference Laboratory for Brucellosis, Bacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea.
authors.forename: So-Ra
authors.initials: SR
authors.lastname: Sung
authors.affiliation: OIE Reference Laboratory for Brucellosis, Bacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea.
authors.forename: Jin Ju
authors.initials: JJ
authors.lastname: Lee
authors.affiliation: OIE Reference Laboratory for Brucellosis, Bacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea.
authors.forename: Suk Chan
authors.initials: SC
authors.lastname: Jung
authors.affiliation: Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 151-742, Republic of Korea.
authors.forename: Yong Ho
authors.initials: YH
authors.lastname: Park
authors.affiliation: OIE Reference Laboratory for Brucellosis, Bacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea; Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 151-742, Republic of Korea. Electronic address: kimjiyeon75@korea.kr.
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Kim
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002002:Brucella / Q000235:genetics / Q000302:isolation & purification; D002004:Brucella Vaccine; D017347:Brucella melitensis / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification*; D002006:Brucellosis / Q000453:epidemiology* / Q000382:microbiology* / Q000635:transmission / Q000662:veterinary; D002681:China / Q000453:epidemiology; D004269:DNA, Bacterial / Q000235:genetics; D005838:Genotype; D015511:Goat Diseases / Q000453:epidemiology / Q000382:microbiology; D006041:Goats; D006801:Humans; D017720:Molecular Epidemiology; D008986:Mongolia / Q000453:epidemiology; D058885:Multilocus Sequence Typing; D060885:Multiplex Polymerase Chain Reaction; D010802:Phylogeny; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology / Q000382:microbiology; D034561:Sheep, Domestic; D015047:Zoonoses
chemical_list: D002004:Brucella Vaccine; D004269:DNA, Bacterial
keywords: Brucella melitensis; China; Epidemiology; MLVA-16; Mongolia; Zoonoses