title: CRISPR/Cas 9 genome editing and its applications in organoids.
abstract: Organoids are three-dimensional (3D) structures derived from adult or embryonic stem cells that maintain many structural and functional features of their respective organ. Recently, genome editing based on the bacterial defense mechanism CRISPR/Cas9 has emerged as an easily applicable and reliable laboratory tool. Combining organoids and CRISPR/Cas9 creates exciting new opportunities to study organ development and human disease in vitro. The potential applications of CRISPR in organoids are only beginning to be explored.
authors.affiliation: Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW), Utrecht, The Netherlands.|University Medical Center (UMC) Utrecht, Utrecht, The Netherlands; and.
authors.forename: Else
authors.initials: E
authors.lastname: Driehuis
authors.affiliation: Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW), Utrecht, The Netherlands; h.clevers@hubrecht.eu.|University Medical Center (UMC) Utrecht, Utrecht, The Netherlands; and.|Princess Maxima Center, Utrecht, The Netherlands.
authors.forename: Hans
authors.initials: H
authors.lastname: Clevers
mesh_terms: D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D000072669:Gene Editing; D006801:Humans; D009940:Organoids
keywords: CRISPR/Cas9; gene editing; organoid