title: Preparation and Characterization of a Chitosan/Gelatin/Extracellular Matrix Scaffold and Its Application in Tissue Engineering.
abstract: Previous studies have demonstrated that extracellular matrix (ECM) can be used in tissue engineering due to its bioactivity. However, adipose-derived ECM (A-dECM) has never been applied in bone tissue engineering, and it is unknown whether it would be beneficial to the growth of bone marrow mesenchymal stem cells (BMSCs). In this study, we produced chitosan/gelatin/A-dECM (C/G/A-dECM) scaffolds via lyophilization and crosslinking; chitosan/gelatin (C/G) scaffolds were used as controls. For the C/G/A-dECM scaffolds, the average pore size was 285.93 ± 85.39 μm; the average porosity was 90.62 ± 3.65%; the average compressive modulus was 0.87 ± 0.05 kPa; and the average water uptake ratio was 13.73 ± 1.16. In vitro, A-dECM scaffolds could promote the attachment and proliferation of BMSCs. In the same osteogenic-inducing reagent, better osteogenic differentiation could be observed for the C/G/A-dECM scaffolds than for the C/G scaffolds. Thus, we conclude that A-dECM is a promising material and that C/G/A-dECM scaffolds are a candidate for bone tissue engineering.
authors.affiliation: 1 Department of Orthopedics, The Second Afflicated Hospital of Harbin Medical University , Harbin, China .|2 Key Laboratory of the Ministry of Education , Ministry of Myocardial Ischemia, Harbin, China .
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
authors.affiliation: 1 Department of Orthopedics, The Second Afflicated Hospital of Harbin Medical University , Harbin, China .|2 Key Laboratory of the Ministry of Education , Ministry of Myocardial Ischemia, Harbin, China .
authors.forename: Tailong
authors.initials: T
authors.lastname: Yu
authors.affiliation: 1 Department of Orthopedics, The Second Afflicated Hospital of Harbin Medical University , Harbin, China .|2 Key Laboratory of the Ministry of Education , Ministry of Myocardial Ischemia, Harbin, China .
authors.forename: Guanghua
authors.initials: G
authors.lastname: Chen
authors.affiliation: 3 Department of Orthopedics, The First Afflicated Hospital of Harbin Medical University , Harbin, China .
authors.forename: Jilong
authors.initials: J
authors.lastname: Zou
authors.affiliation: 2 Key Laboratory of the Ministry of Education , Ministry of Myocardial Ischemia, Harbin, China .|4 Department of Cardiac Surgery, The Second Afflicated Hospital of Harbin Medical University , Harbin, China .
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Li
authors.affiliation: 1 Department of Orthopedics, The Second Afflicated Hospital of Harbin Medical University , Harbin, China .
authors.forename: Jinglong
authors.initials: J
authors.lastname: Yan
mesh_terms: D000818:Animals; D001853:Bone Marrow; D002454:Cell Differentiation; D048271:Chitosan / Q000737:chemistry*; D005109:Extracellular Matrix / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D005780:Gelatin; D048271:Chitosan
keywords: BMSCs; extracellular matrix; osteogenic differentiation; tissue engineering