title: Deregulated lncRNAs in B Cells from Patients with Active Tuberculosis.
abstract: Role of lncRNAs in human adaptive immune response to TB infection is largely unexplored. To address this issue, here we characterized lncRNA expression profile in primary human B cell response to TB infection using microarray assay. Several lncRNAs and mRNAs were chosen for RT-qPCR validation. Bioinformatics prediction was applied to delineate function of the deregulated mRNAs. We found that 844 lncRNAs and 597 mRNAs were differentially expressed between B cell samples from individuals with or without TB. KEGG pathway analysis for the deregulated mRNAs indicated a number of pathways, such as TB, TLR signaling pathway and antigen processing and presentation. Moreover, corresponding to the dysregulation of many lncRNAs, we also found that their adjacent protein-coding genes were also deregulated. Functional annotation for the corresponding mRNAs showed that these lncRNAs were mainly associated with TLR signaling, TGF-β signaling. Interestingly, SOCS3, which is a critical negative regulator of cytokine response to TB infection and its nearby lncRNA XLOC_012582, were highly expressed in active TB B cells. Subsequent RT-qPCR results confirmed the changes. Whether upregulated XLOC_012582 causes SOCS3 overexpression and is eventually involved in the context of exacerbations of active TB represents an interesting issue that deserves to be further explored. Taken together, for the first time, we identified a set of deregulated lncRNAs in active TB B cells and their functions were predicted. Such findings provided novel insight into the pathogenesis of TB and further studies should focus on the function and pathogenic mechanisms of the lncRNAs involved in active TB.
authors.affiliation: Department of Medical Microbiology of Clinical Medicine College, Weifang Medical University, Shandong, Weifang, China.|Department of Laboratory Medicine, Key Laboratory of Clinical Laboratory Diagnostics in Universities of Shandong, Weifang Medical University, Shandong, Weifang, China.
authors.forename: Yurong
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Department of Clinical Skill Laboratory of Clinical Medicine College, Weifang Medical University, Shandong, Weifang, China.
authors.forename: Xianqin
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Surgery of Gaomi City people's Hospital, Shandong, Weifang, China.
authors.forename: Junfang
authors.initials: J
authors.lastname: Xue
authors.affiliation: Department of Nursing of Affiliated Hospital of Weifang Medical University, Shandong, Weifang, China.
authors.forename: Wenping
authors.initials: W
authors.lastname: Duan
authors.affiliation: Department of Laboratory Medicine, Key Laboratory of Clinical Laboratory Diagnostics in Universities of Shandong, Weifang Medical University, Shandong, Weifang, China.
authors.forename: Zhengjun
authors.initials: Z
authors.lastname: Yi
mesh_terms: D056704:Adaptive Immunity; D000328:Adult; D017951:Antigen Presentation; D001402:B-Lymphocytes / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D016022:Case-Control Studies; D019295:Computational Biology; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D063990:Gene Ontology; D006801:Humans; D008297:Male; D046228:Microarray Analysis; D008875:Middle Aged; D058977:Molecular Sequence Annotation; D009169:Mycobacterium tuberculosis / Q000254:growth & development / Q000276:immunology; D061251:Primary Cell Culture; D062085:RNA, Long Noncoding / Q000235:genetics / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics / Q000276:immunology*; D015398:Signal Transduction; D000071223:Suppressor of Cytokine Signaling 3 Protein / Q000235:genetics / Q000276:immunology*; D051193:Toll-Like Receptors / Q000235:genetics / Q000276:immunology; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000235:genetics / Q000276:immunology* / Q000382:microbiology / Q000473:pathology
chemical_list: D062085:RNA, Long Noncoding; D012333:RNA, Messenger; C106644:SOCS3 protein, human; D000071223:Suppressor of Cytokine Signaling 3 Protein; D051193:Toll-Like Receptors; D016212:Transforming Growth Factor beta