title: De NovoTranscriptome Characterization of a Sterilizing Trematode Parasite (Microphallussp.) from Two Species of New Zealand Snails.
abstract: Snail-borne trematodes represent a large, diverse, and evolutionarily, ecologically, and medically important group of parasites, often imposing strong selection on their hosts and causing host morbidity and mortality. Even so, there are very few genomic and transcriptomic resources available for this important animal group. We help to fill this gap by providing transcriptome resources from trematode metacercariae infecting two congeneric snail species,Potamopyrgus antipodarumandP. estuarinusThis genus of New Zealand snails has gained prominence in large part through the development ofP. antipodarumand its sterilizing trematode parasiteMicrophallus livelyiinto a textbook model for host-parasite coevolutionary interactions in nature. By contrast, the interactions betweenMicrophallustrematodes andP. estuarinus, an estuary-inhabiting species closely related to the freshwaterP. antipodarum, are relatively unstudied. Here, we provide the first annotated transcriptome assemblies fromMicrophallusisolated fromP. antipodarumandP. estuarinusWe also use these transcriptomes to produce genomic resources that will be broadly useful to those interested in host-parasite coevolution, local adaption, and molecular evolution and phylogenetics of this and other snail-trematode systems. Analyses of the twoMicrophallustranscriptomes revealed that the two trematode types are more genetically differentiated from one another than are theM. livelyiinfecting different populations ofP. antipodarum, suggesting that theMicrophallusinfectingP. estuarinusrepresent a distinct lineage. We also provide a promising set of candidate genes likely involved in parasitic infection and response to salinity stress.
authors.affiliation: Department of Biology, University of Iowa, Iowa City, Iowa 52242 laura-rice@uiowa.edu.
authors.forename: Laura
authors.initials: L
authors.lastname: Bankers
authors.affiliation: Department of Biology, University of Iowa, Iowa City, Iowa 52242.
authors.forename: Maurine
authors.identifier: 0000-0002-1543-8115
authors.initials: M
authors.lastname: Neiman
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000378:metabolism; D056426:Genetic Loci; D018895:Microsatellite Repeats / Q000235:genetics; D058977:Molecular Sequence Annotation; D009520:New Zealand; D010271:Parasites / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012908:Snails / Q000469:parasitology*; D013242:Sterilization; D059467:Transcriptome / Q000235:genetics*; D014200:Trematoda / Q000235:genetics*
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger
keywords: coevolution; host-parasite interactions; microsatellites; transcriptome; trematode