title: TheSaccharomyces cerevisiaeCdk8 Mediator RepressesAQY1Transcription by Inhibiting Set1p-Dependent Histone Methylation.
abstract: In the budding yeastSaccharomyces cerevisiae, nutrient depletion induces massive transcriptional reprogramming that relies upon communication between transcription factors, post-translational histone modifications, and the RNA polymerase II holoenzyme complex. Histone H3Lys4 methylation (H3Lys4 me), regulated by the Set1p-containing COMPASS methyltransferase complex and Jhd2p demethylase, is one of the most well-studied histone modifications. We previously demonstrated that the RNA polymerase II mediator components cyclin C-Cdk8p inhibit locus-specific H3Lys4 3me independently of Jhd2p Here, we identify loci subject to cyclin C- and Jhd2p-dependent histone H3Lys4 3me inhibition using chromatin immunoprecipitation (ChIP)-seq. We further characterized the independent and combined roles of cyclin C and Jhd2p in controlling H3Lys4 3me and transcription in response to fermentable and nonfermentable carbon at multiple loci. These experiments suggest that H3Lys4 3me alone is insufficient to induce transcription. Interestingly, we identified an unexpected role for cyclin C-Cdk8p in repressingAQY1transcription, an aquaporin whose expression is normally induced during nutrient deprivation. These experiments, combined with previous work in other labs, support a two-step model in which cyclin C-Cdk8p mediateAQY1transcriptional repression by stimulating transcription factor proteolysis and preventing Set1p recruitment to theAQY1locus.
authors.affiliation: Department of Molecular Biology, Rowan University School of Osteopathic Medicine, Stratford, New Jersey 08084 lawmj@rowan.edu.
authors.forename: Michael J
authors.identifier: 0000-0001-8976-7459
authors.initials: MJ
authors.lastname: Law
authors.affiliation: Department of Molecular Biology, Rowan University School of Osteopathic Medicine, Stratford, New Jersey 08084.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Finger
mesh_terms: D020346:Aquaporins / Q000235:genetics*; D056850:Cyclin-Dependent Kinase 8 / Q000378:metabolism*; D005285:Fermentation / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D056426:Genetic Loci; D011495:Histone-Lysine N-Methyltransferase / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D008745:Methylation; D008954:Models, Biological; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*; D013312:Stress, Physiological / Q000235:genetics; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
chemical_list: C115153:AQY1 protein, S cerevisiae; D020346:Aquaporins; D006657:Histones; D029701:Saccharomyces cerevisiae Proteins; D011495:Histone-Lysine N-Methyltransferase; C411250:SET1 protein, S cerevisiae; D056850:Cyclin-Dependent Kinase 8; C498309:SSN3 protein, S cerevisiae
keywords: histone modifications; mediator; transcription factors