title: Diagnostic accuracy of an in-house ELISA using the intermediate species Leptospira fainei as antigen for diagnosis of acute leptospirosis in dogs.
abstract: Diagnosis of animal leptospirosis is still challenging. The microscopic agglutination test, is the current method for diagnosing leptospirosis. However, this technique requires specific equipment, highly trained staff and the maintenance of live cultures of several reference strains of Leptospira for use as antigens. Recently, an ELISA (enzyme-linked immunosorbent assay) employing a Leptospira fainei serovar Hurstbridge based antigen for the early diagnostic of human leptospirosis was developed. In this study we estimate the diagnostic sensitivity and specificity of this test in identifying acute canine leptospirosis. A total of 271 serum samples divided into five panels and tested by MAT as a reference test, were used to evaluate the ELISA. Comparing acutely and non-acutely infected dogs, ELISA-Hb showed 95.6% sensitivity and 93% specificity. L. fainei-based ELISA is adequate for diagnosing acute canine leptospirosis, with high sensitivity and specificity and presenting practical advantages when compared to current techniques.
authors.affiliation: Fluminense Federal University, Rio de Janeiro, Brazil. Electronic address: bpenna@id.uff.br.
authors.forename: Bruno
authors.initials: B
authors.lastname: Penna
authors.affiliation: Fluminense Federal University, Rio de Janeiro, Brazil.
authors.forename: Carla D
authors.initials: CD
authors.lastname: Marassi
authors.affiliation: Fluminense Federal University, Rio de Janeiro, Brazil.
authors.forename: Hugo
authors.initials: H
authors.lastname: Libonati
authors.affiliation: Fluminense Federal University, Rio de Janeiro, Brazil.
authors.forename: Lorena
authors.initials: L
authors.lastname: Narduche
authors.affiliation: Fluminense Federal University, Rio de Janeiro, Brazil.
authors.forename: Walter
authors.initials: W
authors.lastname: Lilenbaum
authors.affiliation: Institut Pasteur, Paris, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Bourhy
mesh_terms: D000208:Acute Disease; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial; D004283:Dog Diseases / Q000175:diagnosis* / Q000276:immunology; D004285:Dogs / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D007919:Leptospira / Q000145:classification / Q000276:immunology*; D007922:Leptospirosis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D012680:Sensitivity and Specificity; D065288:Serogroup
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007075:Immunoglobulin M
keywords: Diagnosis; Dogs; ELISA; Leptospira; Leptospirosis; Sensitivity; Specificity