title: Can Resistance Exercise Alter Irisin Levels and Expression Profiles ofFNDC5andUCP1in Rats?
abstract: BACKGROUND
  Irisin is a new myokine secreted from the skeletal muscle and appears to affect the metabolism of adipose tissue.
  
  
  OBJECTIVE
  The mechanisms of cellular and molecular identification by which exercise training exerts its benefits remain unclear and are under investigation.
  
  
  METHODS
  We examined the effect of 8-week resistance exercise on plasma irisin levels and expression profiles of muscleFNDC5and subcutaneous adipose tissueUCP1in male rats. Sixteen adult male rats were divided into two groups, control (n = 8) and exercise training (n = 8) groups. The training group received exercise for 3 days/week on a specific ladder (120 cm height) with a carrying load of 50% of body weight, which was attached to their tails.
  
  
  RESULTS
  The weight of the load was gradually increased during the training sessions, ultimately reaching 200% of the body weight of rats in the final week. There were three sets of five repetitions with a 3-min rest between each set of exercise sessions and 1 minute between repetitions. Plasma irisin levels and relative mRNA expression of the genesUCP1andFNDC5were assessed. The results demonstrated a significant increase in the irisin levels after 8 weeks of resistance exercise (P < 0.001, t = 4.48). The relative expression ofFNDC5(P < 0.001, t = 6.18) andUCP1genes (P < 0.001, t = 13.91) was also significantly increased.
  
  
  CONCLUSIONS
  Therefore, we can conclude from this study that resistance exercise may improve body composition possibly through increased thermogenesis in white adipose tissue through the secretion of irisin.
authors.affiliation: Department of Exercise Physiology, Faculty of Sport Sciences, University of Isfahan, Isfahan, Iran.
authors.forename: Jalil
authors.initials: J
authors.lastname: Reisi
authors.affiliation: Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran; Department of Cellular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
authors.forename: Kamran
authors.initials: K
authors.lastname: Ghaedi
authors.affiliation: Department of Exercise Physiology, Faculty of Sport Sciences, University of Kharazmi, Tehran, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Rajabi
authors.affiliation: Department of Exercise Physiology, Faculty of Sport Sciences, University of Isfahan, Isfahan, Iran.
authors.forename: Sayyed Mohammad
authors.initials: SM
authors.lastname: Marandi
keywords: FNDC5; Irisin Protein; Resistance Training; UCP1