title: Isolation, Characterization, and Degradation Performance of the 17β-Estradiol-Degrading Bacterium Novosphingobium sp. E2S.
abstract: A 17β-estradiol (E2)-degrading bacterium E2S was isolated from the activated sludge in a sewage treatment plant (STP). The morphology, biological characteristics, and 16S ribosomal RNA (rRNA) gene sequence of strain E2S indicated that it belonged to the genusNovosphingobium. The optimal degrading conditions were 30 °C and pH 7.0. The ideal inoculum volume was 5% (v/v), and a 20-mL degradation system was sufficient to support the removal ability of strain E2S. The addition of extra NaCl to the system did not benefit the E2 degradation in batch culture by this strain. Strain E2S exhibited high degradation efficiency with initial substrate concentrations of 10-50 mg·L-1. For example, in mineral salt medium containing 50 mg·L-1of E2, the degradation efficiency was 63.29% after seven days. In cow manure samples supplemented with 50 mg·L-1of E2, strain E2S exhibited 66.40% degradation efficiency after seven days. The finding of the E2-degrading strain E2S provided a promising method for removing E2 from livestock manure in order to reduce the potential environmental risks of E2.
authors.affiliation: Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China. 2015103034@njau.edu.cn.
authors.forename: Shunyao
authors.initials: S
authors.lastname: Li
authors.affiliation: Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China. liujuan@njau.edu.cn.
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China. 2013103044@njau.edu.cn.
authors.forename: Minxia
authors.initials: M
authors.lastname: Sun
authors.affiliation: Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China. lingwanting@njau.edu.cn.
authors.forename: Wanting
authors.initials: W
authors.lastname: Ling
authors.affiliation: Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China. zhuxuezhu@njau.edu.cn.
authors.forename: Xuezhu
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001673:Biodegradation, Environmental; D002417:Cattle; D002681:China; D004958:Estradiol / Q000378:metabolism*; D004967:Estrogens / Q000378:metabolism*; D005260:Female; D008372:Manure / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D045249:Sphingobacterium / Q000235:genetics* / Q000378:metabolism*
chemical_list: D004967:Estrogens; D008372:Manure; D012336:RNA, Ribosomal, 16S; D004958:Estradiol
keywords: 17β-estradiol; Novosphingobium sp.; cow manure; degradation; estrogen