title: Molecular Analysis of a Genetic Variants Panel Related to Nutrients and Metabolism: Association with Susceptibility to Gestational Diabetes and Cardiometabolic Risk in Affected Women.
abstract: Gestational diabetes mellitus (GDM) is the most frequent metabolic disorder in pregnancy. Women with a GDM history are at increased risk of developing diabetes and cardiovascular diseases. Studies have demonstrated a significant correlation between several genes involved in the metabolic pathway of insulin and environmental factors. The aim of this study was to investigate the relationship between clinical parameters in GDM and variants in genes involved with nutrients and metabolism. Several variantsPPARG2rs1801282 (C>G);PPARGC1Ars8192678 (C>T);TCF7L2rs7903146 (C>T);LDLRrs2228671 (C>T);MTHFRrs1801133 (C>T);APOA5rs662799 (T>C);GCKRrs1260326 (C>T);FTOrs9939609 (T>A);MC4Rrs17782313 (T>C) were genotyped in 168 pregnant Caucasian women with or without GDM by High Resolution Melting (HRM) analysis. A significant correlation was observed between TT genotype ofTCF7L2gene and increased risk of GDM (OR 5.4 [95% CI 1.5-19.3]). Moreover, a significant correlation was observed between lipid parameters and genetic variations in additional genes, namely,PPARG2[p= 0,02],APOA5[p= 0,02],MC4R[p= 0,03],LDLR[p= 0,01], andFTO[p= 0,02]. Our findings support the association betweenTCF7L2rs7903146 variant and an increased GDM risk. Results about the investigated genetic variants provide important information about cardiometabolic risk in GDM and help to plan future prevention studies.
authors.affiliation: Laboratory of Molecular Genetics, Department of Psychological, Health and Territorial Sciences, School of Medicine and Health Sciences, "G. d'Annunzio" University, Chieti-Pescara, Via dei Vestini 31, 66013 Chieti, Italy; Ce.S.I-Met, "G. d'Annunzio" University, Chieti-Pescara, Via Colle dell'Ara No. 1, 66100 Chieti, Italy.
authors.forename: Marica
authors.identifier: 0000-0002-4830-8034
authors.initials: M
authors.lastname: Franzago
authors.affiliation: Ce.S.I-Met, "G. d'Annunzio" University, Chieti-Pescara, Via Colle dell'Ara No. 1, 66100 Chieti, Italy; Department of Medicine and Aging, School of Medicine and Health Sciences, "G. d'Annunzio" University, Chieti-Pescara, Chieti, Italy.
authors.forename: Federica
authors.identifier: 0000-0002-8169-1864
authors.initials: F
authors.lastname: Fraticelli
authors.affiliation: Center for Outcomes Research and Clinical Epidemiology (CORE), Pescara, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Nicolucci
authors.affiliation: Department of Medicine and Aging, School of Medicine and Health Sciences, "G. d'Annunzio" University, Chieti-Pescara, Chieti, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Celentano
authors.affiliation: Department of Medicine and Aging, School of Medicine and Health Sciences, "G. d'Annunzio" University, Chieti-Pescara, Chieti, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Liberati
authors.affiliation: Laboratory of Molecular Genetics, Department of Psychological, Health and Territorial Sciences, School of Medicine and Health Sciences, "G. d'Annunzio" University, Chieti-Pescara, Via dei Vestini 31, 66013 Chieti, Italy; Ce.S.I-Met, "G. d'Annunzio" University, Chieti-Pescara, Via Colle dell'Ara No. 1, 66100 Chieti, Italy.
authors.forename: Liborio
authors.initials: L
authors.lastname: Stuppia
authors.affiliation: Ce.S.I-Met, "G. d'Annunzio" University, Chieti-Pescara, Via Colle dell'Ara No. 1, 66100 Chieti, Italy; Department of Medicine and Aging, School of Medicine and Health Sciences, "G. d'Annunzio" University, Chieti-Pescara, Chieti, Italy.
authors.forename: Ester
authors.identifier: 0000-0003-1756-661X
authors.initials: E
authors.lastname: Vitacolonna
mesh_terms: D000328:Adult; D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO / Q000235:genetics; D000072040:Apolipoprotein A-V / Q000235:genetics; D016640:Diabetes, Gestational / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D047495:PPAR gamma / Q000235:genetics; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D044105:Receptor, Melanocortin, Type 4 / Q000235:genetics; D011973:Receptors, LDL / Q000235:genetics; D012307:Risk Factors; D058118:Transcription Factor 7-Like 2 Protein / Q000235:genetics
chemical_list: D000072040:Apolipoprotein A-V; D047495:PPAR gamma; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D044105:Receptor, Melanocortin, Type 4; D011973:Receptors, LDL; C544894:TCF7L2 protein, human; D058118:Transcription Factor 7-Like 2 Protein; D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO; C526492:FTO protein, human