title: MicroRNA-101 inhibits proliferation, migration and invasion in osteosarcoma cells by targeting ROCK1.
abstract: Osteosarcoma is a rare malignant bone tumor in adolescents, with high degree of malignancy, and highly incidence of recurrence and metastasis. Our study aimed to explore the role of miR-101 in osteosarcoma cells by targeting ROCK1. In the present study, reverse transcription-quantitative polymerase chain reaction data revealed that miR-101 was down-regulated in the tissue samples of 20 patients with osteosarcoma compared with their matched adjacent non-tumor tissues (P< 0.01). Furthermore, miR-101 was significantly down-regulated in three common OS cell lines, MG63, U2OS, and OS732 compared with the human osteoblast cell line, hFOB1.19 (P< 0.01). MiR-101 was shown to target the ROCK1 3'-UTR in dual-luciferase reporter assays in MG63 cells. Overexpression of miR-101 significantly suppressed the protein expression levels of ROCK1, while knockdown of miR-101 significantly enhanced the formers' expression levels in MG63 cells (P< 0.05). Overexpression of miR-101 inhibited cell viability, migration, and invasion while promoted apoptosis. Independent inhibition of ROCK1 and knockdown of miR-101 expression levels significantly promoted MG63 cell proliferation, migration and invasion while inhibited apoptosis (P< 0.01). Moreover, knockdown of ROCK1 reversed the promotion effect of miR-101 knockdown on proliferation, migration, and invasion while promoted apoptosis of MG63 cells, suggesting that miR-101 acts as a tumor suppressor in osteosarcoma cells via targeting ROCK1. Furthermore, overexpression of miR-101 inhibited tumor growth and motion by inactivating PI3K/AKT and JAK/STAT signaling pathways via downregulation of ROCK1. To conclude, miR-101/ROCK1 may be a potential therapeutic target for osteosarcoma therapy.
authors.affiliation: Department of Orthopedics, China-Japan Union Hospital of Jilin University Changchun 130033, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Jiang
authors.affiliation: Department of Ophthalmology, The Second Hospital of Jilin University Changchun 130041, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, China-Japan Union Hospital of Jilin University Changchun 130033, China.
authors.forename: Guangyao
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, China-Japan Union Hospital of Jilin University Changchun 130033, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Gu
authors.affiliation: Department of Orthopedics, China-Japan Union Hospital of Jilin University Changchun 130033, China.
authors.forename: Han
authors.initials: H
authors.lastname: Wu
keywords: Osteosarcoma; ROCK1; cell apoptosis; cell migration; microRNA-101