title: Epithelial response to a high-protein diet in rat colon.
abstract: High-protein diets (HPD) alter the large intestine microbiota composition in association with a metabolic shift towards protein degradation. Some amino acid-derived metabolites produced by the colon bacteria are beneficial for the mucosa while others are deleterious at high concentrations. The aim of the present work was to define the colonic epithelial response to an HPD. Transcriptome profiling was performed on colonocytes of rats fed an HPD or an isocaloric normal-protein diet (NPD) for 2 weeks.
  
  
  
  The HPD downregulated the expression of genes notably implicated in pathways related to cellular metabolism, NF-κB signaling, DNA repair, glutathione metabolism and cellular adhesion in colonocytes. In contrast, the HPD upregulated the expression of genes related to cell proliferation and chemical barrier function. These changes at the mRNA level in colonocytes were not associated with detrimental effects of the HPD on DNA integrity (comet assay), epithelium renewal (quantification of proliferation and apoptosis markers by immunohistochemistry and western blot) and colonic barrier integrity (Ussing chamber experiments).
  
  
  
  The modifications of the luminal environment after an HPD were associated with maintenance of the colonic homeostasis that might be the result of adaptive processes in the epithelium related to the observed transcriptional regulations.
authors.affiliation: UMR Physiologie de la Nutrition et du Comportement Alimentaire, AgroParisTech, INRA, Université Paris-Saclay, 16 rue Claude Bernard, 75005, Paris, France.
authors.forename: Martin
authors.initials: M
authors.lastname: Beaumont
authors.affiliation: UMR Physiologie de la Nutrition et du Comportement Alimentaire, AgroParisTech, INRA, Université Paris-Saclay, 16 rue Claude Bernard, 75005, Paris, France.
authors.forename: Mireille
authors.initials: M
authors.lastname: Andriamihaja
authors.affiliation: UMR Physiologie de la Nutrition et du Comportement Alimentaire, AgroParisTech, INRA, Université Paris-Saclay, 16 rue Claude Bernard, 75005, Paris, France.
authors.forename: Lucie
authors.initials: L
authors.lastname: Armand
authors.affiliation: UMR Physiologie de la Nutrition et du Comportement Alimentaire, AgroParisTech, INRA, Université Paris-Saclay, 16 rue Claude Bernard, 75005, Paris, France.
authors.forename: Marta
authors.initials: M
authors.lastname: Grauso
authors.affiliation: UMR1313 Génétique Animale et Biologie Intégrative, INRA, 78350, Jouy-en-Josas, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Jaffrézic
authors.affiliation: UMR1313 Génétique Animale et Biologie Intégrative, INRA, 78350, Jouy-en-Josas, France.
authors.forename: Denis
authors.initials: D
authors.lastname: Laloë
authors.affiliation: CRB GADIE, INRA, 78350, Jouy-en-Josas, France.
authors.forename: Marco
authors.initials: M
authors.lastname: Moroldo
authors.affiliation: UMR Physiologie de la Nutrition et du Comportement Alimentaire, AgroParisTech, INRA, Université Paris-Saclay, 16 rue Claude Bernard, 75005, Paris, France.
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Davila
authors.affiliation: UMR Physiologie de la Nutrition et du Comportement Alimentaire, AgroParisTech, INRA, Université Paris-Saclay, 16 rue Claude Bernard, 75005, Paris, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Tomé
authors.affiliation: UMR Physiologie de la Nutrition et du Comportement Alimentaire, AgroParisTech, INRA, Université Paris-Saclay, 16 rue Claude Bernard, 75005, Paris, France.
authors.forename: François
authors.initials: F
authors.lastname: Blachier
authors.affiliation: UMR Physiologie de la Nutrition et du Comportement Alimentaire, AgroParisTech, INRA, Université Paris-Saclay, 16 rue Claude Bernard, 75005, Paris, France. annaig.lan@agroparistech.fr.
authors.forename: Annaïg
authors.initials: A
authors.lastname: Lan
mesh_terms: D000821:Animal Feed; D000818:Animals; D016000:Cluster Analysis; D003106:Colon / Q000378:metabolism*; D004032:Diet; D004044:Dietary Proteins / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005978:Glutathione / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D051381:Rats; D015398:Signal Transduction; D059467:Transcriptome
chemical_list: D004044:Dietary Proteins; D005978:Glutathione
keywords: Barrier function; Colon; DNA damages; Dietary protein; Epithelial cells; Epithelial renewal; High-protein diet; Mucus; Transcriptome