title: Influence of Estradiol-17beta on Progesterone and Estrogen Receptor mRNA Expression in Porcine Follicular Granulosa Cells during Short-Term,In VitroReal-Time Cell Proliferation.
abstract: Progesterone (P4) and estradiol (E2) play a significant role in mammalian reproduction. Our study demonstrated that separated porcine cumulus cells (CCs) and/or granulosa cells (GCs) might proliferatein vitroduring short-term, real-time primary culture. The GCs were analyzed according to gene expression of the progesterone receptor (nuclear form) (pgr), progesterone receptor membrane component 1 (pgrmc1), and estrogen-related receptor beta 3 (esrrb3) in relation to two housekeeping genes:actbandpbgd. GCs were cultivated in medium with the E2. Bothpgr/actbandpgr/pbgdrevealed higher expression between 24 and 168 h of IVC of prolonged E2 treatment and at 48 h of IVC after acute E2 administration. Thepgrmc1/actbandpgrmc1/pbgddisplayed increased expression after prolonged E2 treatment between 24 and 120 h of IVC. The highest level ofesrrb3/actbat 120 and 144 h, as well asesrrb3/pbgdat 120 h, in untreated controls as compared to the hormone-stimulated group, was observed. We suggest that E2 significantly influences the upregulation ofpgr,pgrmc1, andesrrb3expression in porcine GCs during real-time cell proliferation. Sinceesrrb3expression is stimulated by E2 in both an acute and prolonged manner, estradiol may be recognized as a potential estrogen receptor agonist in GCs.
authors.affiliation: Department of Histology and Embryology, Poznań University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Ciesiółka
authors.affiliation: Department of Histology and Embryology, Poznań University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Budna
authors.affiliation: Department of Histology and Embryology, Poznań University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland.
authors.forename: Karol
authors.initials: K
authors.lastname: Jopek
authors.affiliation: Department of Anatomy, Poznań University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland.
authors.forename: Artur
authors.initials: A
authors.lastname: Bryja
authors.affiliation: Department of Anatomy, Poznań University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland.
authors.forename: Wiesława
authors.initials: W
authors.lastname: Kranc
authors.affiliation: Department of Histology and Embryology, Poznań University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland.
authors.forename: Adrian
authors.initials: A
authors.lastname: Chachuła
authors.affiliation: Department of Anatomy, Poznań University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Borys
authors.affiliation: Department of Biomaterials and Experimental Dentistry, Poznań University of Medical Sciences, 70 Bukowska St., 60-812 Poznań, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Dyszkiewicz Konwińska
authors.affiliation: Department of Histology and Embryology, Poznań University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland; Department of Anatomy and Histology, Faculty of Medicine and Health Sciences, University of Zielona Gora, ul. Zyty 28, 65-046 Zielona Gora, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Ziółkowska
authors.affiliation: Institute of Veterinary and Animal Science, Poznań University of Life Sciences, 52 Wojska Polskiego St., 60-628 Poznań, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Antosik
authors.affiliation: Institute of Veterinary and Animal Science, Poznań University of Life Sciences, 52 Wojska Polskiego St., 60-628 Poznań, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Bukowska
authors.affiliation: Institute of Veterinary and Animal Science, Poznań University of Life Sciences, 52 Wojska Polskiego St., 60-628 Poznań, Poland.
authors.forename: Klaus P
authors.initials: KP
authors.lastname: Brüssow
authors.affiliation: Department of Anatomy, Poznań University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Bruska
authors.affiliation: Department of Histology and Embryology, Poznań University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Nowicki
authors.affiliation: Department of Histology and Embryology, Poznań University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland; Department of Histology and Embryology, Wroclaw Medical University, 6a Chalubinskiego St., 50-368 Wroclaw, Poland.
authors.forename: Maciej
authors.identifier: 0000-0003-2004-0010
authors.initials: M
authors.lastname: Zabel
authors.affiliation: Department of Histology and Embryology, Poznań University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland; Department of Anatomy, Poznań University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland.
authors.forename: Bartosz
authors.identifier: 0000-0002-6804-0119
authors.initials: B
authors.lastname: Kempisty
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D004958:Estradiol / Q000008:administration & dosage*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000378:metabolism*; D013552:Swine; D013997:Time Factors
chemical_list: D012333:RNA, Messenger; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D004958:Estradiol