title: Development of a high-throughput opsonophagocytic assay for the determination of functional antibody activity against Streptococcus pyogenes using bioluminescence.
abstract: The lack of standardised protocols for the assessment of functional antibodies has hindered Streptococcus pyogenes research and the development of vaccines. A robust, high throughput opsonophagocytic bactericidal assay to determine protective antibodies in human and rabbit serum has been developed that utilises bioluminescence as a rapid read out.
authors.affiliation: Department of Molecular Medicine & Pathology, School of Medical Sciences, The University of Auckland, Private Bag 92019, Auckland, New Zealand; Maurice Wilkins Centre, The University of Auckland, Private Bag 92019, Auckland, New Zealand.
authors.forename: Natalie
authors.initials: N
authors.lastname: Lorenz
authors.affiliation: Department of Molecular Medicine & Pathology, School of Medical Sciences, The University of Auckland, Private Bag 92019, Auckland, New Zealand; Maurice Wilkins Centre, The University of Auckland, Private Bag 92019, Auckland, New Zealand. Electronic address: mj.loh@auckland.ac.nz.
authors.forename: Jacelyn M S
authors.initials: JM
authors.lastname: Loh
authors.affiliation: Department of Molecular Medicine & Pathology, School of Medical Sciences, The University of Auckland, Private Bag 92019, Auckland, New Zealand; Maurice Wilkins Centre, The University of Auckland, Private Bag 92019, Auckland, New Zealand.
authors.forename: Nicole J
authors.initials: NJ
authors.lastname: Moreland
authors.affiliation: Department of Molecular Medicine & Pathology, School of Medical Sciences, The University of Auckland, Private Bag 92019, Auckland, New Zealand; Maurice Wilkins Centre, The University of Auckland, Private Bag 92019, Auckland, New Zealand. Electronic address: t.proft@auckland.ac.nz.
authors.forename: Thomas
authors.initials: T
authors.lastname: Proft
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D057166:High-Throughput Screening Assays; D006801:Humans; D007159:Immunologic Tests / Q000379:methods*; D008163:Luminescent Measurements; D010587:Phagocytosis; D011817:Rabbits; D013290:Streptococcal Infections / Q000175:diagnosis / Q000382:microbiology; D013297:Streptococcus pyogenes / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial
keywords: Bactericidal assay; Bioluminescence; Correlates of protection; Group A Streptococcus (GAS); Lancefield assay; Protective antibodies