title: Evaluation of TA10 Broth for Recovery ofListeria monocytogenesfrom Ground Beef.
abstract: In 2009, the enrichment broth TA10 was released for simultaneous recovery of Salmonella spp., Listeria monocytogenes, and Escherichia coli O157:H7. This medium was compared with other Salmonella enrichment broths [lactose (LAC) broth, buffered peptone water (BPW), and universal pre-enrichment (UP) broth] for the recovery of heat- and freeze-injured Salmonella spp. in beef by the conventional culture method. There was a significant difference between TA10 and LAC enrichment broths for detecting injured Salmonella spp. In this study, the International Organization for Standardization Listeria pre-enrichment broth (Half-Fraser/Fraser) was compared with TA10 broth for the recovery of L. monocytogenes from ground beef. Ground beef samples were contaminated with single Listeria serovars at levels of 0.096 to 0.001 most probable number/g. Twenty 25 g test portions of the contaminated ground beef were pre-enriched in each broth, and the ISO-11290-1 Listeria official isolation protocol was used thereafter. There was a significant difference between TA10 broth (48 h) and Half-Fraser/Fraser broth (72 h) in the recovery of L. monocytogenes. In addition, the incubation time for TA10 broth was shorter than for Half-Fraser/Fraser broth. The results indicate that TA10 broth should be used instead of Half-Fraser/Fraser broth for analysis of beef that may be contaminated with very low levels of L. monocytogenes.
authors.affiliation: PRIMA Meat Packers Ltd, 635 Nakamukaihara, Tsuchiura, Ibaraki 300-0841, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Kamisaki-Horikoshi
authors.affiliation: PRIMA Meat Packers Ltd, 635 Nakamukaihara, Tsuchiura, Ibaraki 300-0841, Japan.
authors.forename: Yukio
authors.initials: Y
authors.lastname: Okada
authors.affiliation: PRIMA Meat Packers Ltd, 635 Nakamukaihara, Tsuchiura, Ibaraki 300-0841, Japan.
authors.forename: Kazuko
authors.initials: K
authors.lastname: Takeshita
authors.affiliation: PRIMA Meat Packers Ltd, 635 Nakamukaihara, Tsuchiura, Ibaraki 300-0841, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Takada
authors.affiliation: National Agriculture and Food Research Organization, Food Research Institute, Food Hygiene Laboratory, Kannondai-2-1-12, Tsukuba 305-8642, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Kawamoto
authors.affiliation: National Agriculture and Food Research Organization, Food Research Institute, Food Hygiene Laboratory, Kannondai-2-1-12, Tsukuba 305-8642, Japan.
authors.forename: Susumu
authors.initials: S
authors.lastname: Kawasaki
mesh_terms: D016009:Chi-Square Distribution; D003470:Culture Media; D008089:Listeria monocytogenes / Q000302:isolation & purification*; D000069466:Red Meat / Q000382:microbiology*
chemical_list: D003470:Culture Media