title: Single-Molecule Sequencing of theDrosophila serrataGenome.
abstract: Long-read sequencing technology promises to greatly enhancede novoassembly of genomes for nonmodel species. Although the error rates of long reads have been a stumbling block, sequencing at high coverage permits the self-correction of many errors. Here, we sequence andde novoassemble the genome ofDrosophila serrata, a species from themontiumsubgroup that has been well-studied for latitudinal clines, sexual selection, and gene expression, but which lacks a reference genome. Using 11 PacBio single-molecule real-time (SMRT cells), we generated 12 Gbp of raw sequence data comprising ∼65 × whole-genome coverage. Read lengths averaged 8940 bp (NRead50 12,200) with the longest read at 53 kbp. We self-corrected reads using the PBDagCon algorithm and assembled the genome using the MHAP algorithm within the PBcR assembler. Total genome length was 198 Mbp with an N50 just under 1 Mbp. Contigs displayed a high degree of chromosome arm-level conservation with theD. melanogastergenome and many could be sensibly placed on theD. serrataphysical map. We also provide an initial annotation for this genome usingin silicogene predictions that were supported by RNA-seq data.
authors.affiliation: School of Biological Sciences, The University of Queensland, St Lucia, Queensland 4072, Australia.
authors.forename: Scott L
authors.initials: SL
authors.lastname: Allen
authors.affiliation: Department of Evolution and Ecology, University of California, Davis, California 95616.
authors.forename: Emily K
authors.initials: EK
authors.lastname: Delaney
authors.affiliation: Department of Evolution and Ecology, University of California, Davis, California 95616.
authors.forename: Artyom
authors.initials: A
authors.lastname: Kopp
authors.affiliation: School of Biological Sciences, The University of Queensland, St Lucia, Queensland 4072, Australia s.chenoweth@uq.edu.au.
authors.forename: Stephen F
authors.initials: SF
authors.lastname: Chenoweth
mesh_terms: D000818:Animals; D020451:Contig Mapping; D004330:Drosophila / Q000235:genetics*; D005260:Female; D017344:Genes, Insect; D049750:Genome, Insect; D008297:Male; D058977:Molecular Sequence Annotation; D017422:Sequence Analysis, DNA; D000072760:Single Molecule Imaging; D013223:Statistics as Topic
keywords: Celera; Drosophila; PacBio; genome assembly; long reads; montium