title: Fatty acid binding protein regulate antimicrobial function via Toll signaling in Chinese mitten crab.
abstract: Fatty acid binding proteins (FABPs) are members of the lipid binding protein superfamily and play crucial role in fatty acid transport and lipid metabolism. In macrophages, Adipocyte-type FABP is an important mediator of inflammation. However, the immune functions of FABPs in invertebrates are not well understood; here, we obtained the gene structure of Eriocheir sinensis FABP 3 and FABP 9 (EsFABP 3 and EsFABP 9), and compared with EsFABP 10. The mRNA expression profiles show that all three FABPs were significantly up-regulated in hemocytes after being challenged with bacteria. Of the three, EsFABP 3 was the most stable and also the most highly up-regulated. Further studies showed that knockdown of EsFABP 3 led to higher bacterial counts in the hemocyte culture medium and a significant decrease in the mRNA expression of some antimicrobial peptides following bacterial stimulation. Moreover, a subcellular study demonstrated that EsFABP 3 can affect nuclear translocation of the dorsal after Gram-positive bacterial stimulation in hemocytes. These findings support the notion that EsFABP 3 could inhibit bacterial proliferation by regulating antimicrobial peptides expression via the Toll signaling pathway.
authors.affiliation: Laboratory of Invertebrate Immunological Defense & Reproductive Biology, School of Life Science, East China Normal University, Shanghai, China.
authors.forename: Shichuang
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
authors.forename: Youting
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Laboratory of Invertebrate Immunological Defense & Reproductive Biology, School of Life Science, East China Normal University, Shanghai, China.
authors.forename: Xuejie
authors.initials: X
authors.lastname: Li
authors.affiliation: Laboratory of Invertebrate Immunological Defense & Reproductive Biology, School of Life Science, East China Normal University, Shanghai, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China. Electronic address: jyli@shou.edu.cn.
authors.forename: Jiayao
authors.initials: J
authors.lastname: Li
authors.affiliation: Laboratory of Invertebrate Immunological Defense & Reproductive Biology, School of Life Science, East China Normal University, Shanghai, China. Electronic address: wwli@bio.ecnu.edu.cn.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Li
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000378:metabolism; D060829:Arthropod Proteins / Q000235:genetics* / Q000378:metabolism; D003386:Brachyura / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D050556:Fatty Acid-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D007113:Immunity, Innate; D008297:Male; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D013211:Staphylococcus aureus / Q000502:physiology; D051193:Toll-Like Receptors / Q000235:genetics / Q000378:metabolism*; D014733:Vibrio / Q000502:physiology
chemical_list: D023181:Antimicrobial Cationic Peptides; D060829:Arthropod Proteins; D050556:Fatty Acid-Binding Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D051193:Toll-Like Receptors
keywords: Antimicrobial peptide; Bacteria; Dorsal; Eriocheir sinensis; EsFABP3