title: High-fidelity mass analysis unveils heterogeneity in intact ribosomal particles.
abstract: Investigation of the structure, assembly and function of protein-nucleic acid macromolecular machines requires multidimensional molecular and structural biology approaches. We describe modifications to an Orbitrap mass spectrometer, enabling high-resolution native MS analysis of 0.8- to 2.3-MDa prokaryotic 30S, 50S and 70S ribosome particles and the 9-MDa Flock House virus. The instrument's improved mass range and sensitivity readily exposes unexpected binding of the ribosome-associated protein SRA.
authors.affiliation: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands.|Netherlands Proteomics Center, Utrecht, the Netherlands.
authors.forename: Michiel
authors.initials: M
authors.lastname: van de Waterbeemd
authors.affiliation: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands.|Netherlands Proteomics Center, Utrecht, the Netherlands.
authors.forename: Kyle L
authors.initials: KL
authors.lastname: Fort
authors.affiliation: Thermo Fisher Scientific, Bremen, Germany.
authors.forename: Dmitriy
authors.initials: D
authors.lastname: Boll
authors.affiliation: Thermo Fisher Scientific, Bremen, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Reinhardt-Szyba
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas, USA.|Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch, Galveston, Texas, USA.
authors.forename: Andrew
authors.identifier: 0000-0002-2874-5990
authors.initials: A
authors.lastname: Routh
authors.affiliation: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands.|Thermo Fisher Scientific, Bremen, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Makarov
authors.affiliation: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands.|Netherlands Proteomics Center, Utrecht, the Netherlands.
authors.forename: Albert J R
authors.identifier: 0000-0002-2405-4404
authors.initials: AJ
authors.lastname: Heck
mesh_terms: D004926:Escherichia coli / Q000166:cytology*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D030381:Nodaviridae / Q000235:genetics / Q000648:ultrastructure*; D011485:Protein Binding / Q000502:physiology; D062085:RNA, Long Noncoding / Q000378:metabolism*; D054681:Ribosome Subunits, Large, Bacterial / Q000235:genetics / Q000648:ultrastructure*; D054680:Ribosome Subunits, Small, Bacterial / Q000235:genetics / Q000648:ultrastructure*
chemical_list: D062085:RNA, Long Noncoding; C405423:steroid receptor RNA activator