title: Human papillomavirus genotypes associated with cervical precancerous lesions and cancer in the highest area of cervical cancer mortality, Longnan, China.
abstract: BACKGROUND
  The mortality of cervical cancer in Longnan is as high as 39/10 million, ranking first in China.
  
  
  METHODS
  Between 2012 to 2016, 329 samples with cervicitis, cervical intraepithelial neoplasia grade 1 to 3 (CINI to III), and invasive squamous cell carcinoma (SCC) were collected. HPV genotypes were examined with a validated kit for 23 different HPV subtypes.
  
  
  RESULTS
  Compared to cervicitis, the HPV positivity is significantly higher in CINI, CIN II/III, and SCC (38.60%, 74.60%, 87.50% and 89.05%,P <0.001) and the positivity is also higher in SCC compared to CINI (P <0.01). The most frequently detected genotypes were HPV16 in cervicitis, HPV16, 58 and 52 in CINI and CIN II/III, and HPV16, 58 and 18 in SCC groups. HPV16 positivity in cervicitis, CINI, CIN II/III, and SCC patients were 45.46%, 46.81%, 60.32% and 78.69%, respectively. Compared to cervicitis and CINI, the odds ratios (OR) for SCC in HPV16 positive patients were 2.96 (95% confidence interval [CI]: 1.09-8.00,P <0.05) and 4.20 (95% confidence interval [CI]: 2.05-8.61,P <0.001), respectively. In addition, the multiple infections in cervicitis, CINI, CINII/III and SCC group are 9.09%, 27.66%, 26.98% and 25.41% and HPV16 + 58 was the most common combinations.
  
  
  CONCLUSIONS
  These findings highlight the key role of HPV16, 58, 52 and 18 in the development of CIN and SCC in Longnan women and a fully aware of regional differences in HPV genotype distribution are tasks for cervical cancer control and prevention.
authors.affiliation: Medical College of Northwest University for Nationalities, Lanzhou, 730030 People's Republic of China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Medical College of Northwest University for Nationalities, Lanzhou, 730030 People's Republic of China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Guo
authors.affiliation: No.1 Hospital of Longnan City, Longnan, 746000 People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Gansu Provincial Cancer Hospital, Lanzhou, 730050 People's Republic of China.
authors.forename: Tianbin
authors.initials: T
authors.lastname: Si
authors.affiliation: Medical College of Northwest University for Nationalities, Lanzhou, 730030 People's Republic of China.
authors.forename: Shuyan
authors.initials: S
authors.lastname: Pei
authors.affiliation: Medical College of Northwest University for Nationalities, Lanzhou, 730030 People's Republic of China.
authors.forename: Chenjing
authors.initials: C
authors.lastname: Wang
authors.affiliation: Medical College of Northwest University for Nationalities, Lanzhou, 730030 People's Republic of China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Qu
authors.affiliation: Medical College of Northwest University for Nationalities, Lanzhou, 730030 People's Republic of China.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Medical College of Northwest University for Nationalities, Lanzhou, 730030 People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Medical College of Northwest University for Nationalities, Lanzhou, 730030 People's Republic of China.
authors.forename: Cong
authors.initials: C
authors.lastname: Nie
authors.affiliation: Medical College of Northwest University for Nationalities, Lanzhou, 730030 People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhang
keywords: Cervical intraepithelial neoplasia grade I to III; Cervicitis; Human papillomaviruses; Invasive squamous cell carcinoma