title: Capitalizing on disaster: Establishing chromatin specificity behind the replication fork.
abstract: Eukaryotic genomes are packaged into nucleosomal chromatin, and genomic activity requires the precise localization of transcription factors, histone modifications and nucleosomes. Classic work described the progressive reassembly and maturation of bulk chromatin behind replication forks. More recent proteomics has detailed the molecular machines that accompany the replicative polymerase to promote rapid histone deposition onto the newly replicated DNA. However, localized chromatin features are transiently obliterated by DNA replication every S phase of the cell cycle. Genomic strategies now observe the rebuilding of locus-specific chromatin features, and reveal surprising delays in transcription factor binding behind replication forks. This implies that transient chromatin disorganization during replication is a central juncture for targeted transcription factor binding within genomes. We propose that transient occlusion of regulatory elements by disorganized nucleosomes during chromatin maturation enforces specificity of factor binding.
authors.affiliation: Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.|Howard Hughes Medical Institute, Seattle, WA, USA.
authors.forename: Srinivas
authors.initials: S
authors.lastname: Ramachandran
authors.affiliation: Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
authors.forename: Kami
authors.initials: K
authors.lastname: Ahmad
authors.affiliation: Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.|Howard Hughes Medical Institute, Seattle, WA, USA.
authors.forename: Steven
authors.initials: S
authors.lastname: Henikoff
mesh_terms: D000818:Animals; D002453:Cell Cycle; D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D004261:DNA Replication; D044127:Epigenesis, Genetic; D056890:Eukaryota / Q000235:genetics / Q000378:metabolism; D006801:Humans; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D002843:Chromatin; D014157:Transcription Factors
keywords: chromatin; epigenetics