title: Extracellular-like matrices and leukaemia inhibitory factor for in vitro culture of human primordial follicles.
abstract: The possibility of maturing human primordial follicles in vitro would assist fertility restoration without the danger of reseeding malignancies. Leukaemia inhibitory factor (LIF) and certain culture matrices may promote human follicular growth. The present study compared human primordial follicular growth on novel culture matrices, namely human recombinant vitronectin (hrVit), small intestine submucosa (SIS), alginate scaffolds and human recombinant virgin collagen bioengineered in tobacco plant lines (CollPlant). The frozen-thawed ovarian samples that were used had been obtained from girls or young women undergoing fertility preservation. In the first part of the study, 20 samples were cultured for 6 days on hrVit or SIS with basic culture medium alone or supplemented with one of two concentrations of LIF (10ngmL-1and 100ngmL-1), with and without LIF-neutralising antibody. In the second part of the study, 15 samples were cultured for 6 days on alginate scaffolds or CollPlant matrices with basic culture medium. Follicular development was assessed by follicular counts and classification, Ki67 immunohistochemistry and 17β-oestradiol and anti-Müllerian hormone measurements in spent media samples. Primordial follicular growth was not enhanced by LIF. Despite some significant differences among the four matrices, none appeared to have a clear advantage, apart from significantly more Ki67-stained follicles on alginate and CollPlant matrices. Further studies of other culture matrices and medium supplements are needed to obtain an optimal system.
authors.affiliation: Infertility and IVF Unit, Beilinson Women Hospital, Rabin Medical Center, 39 Jabotinski Street, Petach Tikva 49100, Israel.
authors.forename: Assiel J
authors.initials: AJ
authors.lastname: Younis
authors.affiliation: Infertility and IVF Unit, Beilinson Women Hospital, Rabin Medical Center, 39 Jabotinski Street, Petach Tikva 49100, Israel.
authors.forename: Galit
authors.initials: G
authors.lastname: Lerer-Serfaty
authors.affiliation: Infertility and IVF Unit, Beilinson Women Hospital, Rabin Medical Center, 39 Jabotinski Street, Petach Tikva 49100, Israel.
authors.forename: Dana
authors.initials: D
authors.lastname: Stav
authors.affiliation: Infertility and IVF Unit, Beilinson Women Hospital, Rabin Medical Center, 39 Jabotinski Street, Petach Tikva 49100, Israel.
authors.forename: Bethsabee
authors.initials: B
authors.lastname: Sabbah
authors.affiliation: Statistical Consulting Unit, Beilinson Hospital, Rabin Medical Center, 39 Jabotinski Street, Petach Tikva 49100, Israel.
authors.forename: Tzippy
authors.initials: T
authors.lastname: Shochat
authors.affiliation: Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, PO Box 39040, Tel Aviv, Israel.
authors.forename: Gania
authors.initials: G
authors.lastname: Kessler-Icekson
authors.affiliation: Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, PO Box 39040, Tel Aviv, Israel.
authors.forename: Muayad A
authors.initials: MA
authors.lastname: Zahalka
authors.affiliation: Sami Shamoon College of Engineering, PO Box 950, Beer-Sheva 84100, Israel.
authors.forename: Michal
authors.initials: M
authors.lastname: Shachar-Goldenberg
authors.affiliation: Infertility and IVF Unit, Beilinson Women Hospital, Rabin Medical Center, 39 Jabotinski Street, Petach Tikva 49100, Israel.
authors.forename: Avi
authors.initials: A
authors.lastname: Ben-Haroush
authors.affiliation: Infertility and IVF Unit, Beilinson Women Hospital, Rabin Medical Center, 39 Jabotinski Street, Petach Tikva 49100, Israel.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Fisch
authors.affiliation: Infertility and IVF Unit, Beilinson Women Hospital, Rabin Medical Center, 39 Jabotinski Street, Petach Tikva 49100, Israel.
authors.forename: Ronit
authors.initials: R
authors.lastname: Abir
mesh_terms: D054304:Anti-Mullerian Hormone / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media; D004958:Estradiol / Q000378:metabolism; D005109:Extracellular Matrix; D005260:Female; D059247:Fertility Preservation / Q000379:methods; D006801:Humans; D053605:Leukemia Inhibitory Factor; D006080:Ovarian Follicle / Q000254:growth & development*
chemical_list: D003470:Culture Media; D053605:Leukemia Inhibitory Factor; D004958:Estradiol; D054304:Anti-Mullerian Hormone