title: Regular Voluntary Exercise Potentiates Interleukin-1βand Interleukin-18 Secretion by Increasing Caspase-1 Expression in Murine Macrophages.
abstract: Moderate-intensity regular exercise improves proinflammatory responses of lipopolysaccharide- (LPS-) stimulated macrophages. However, intracellular events that mediate the beneficial effects of exercise were unclear. This study aimed to clarify the mechanism by which regular voluntary exercise (VE) improves proinflammatory cytokine production by macrophages challenged with LPS. Peritoneal macrophages from VE mice secreted considerably higher amounts of interleukin- (IL-) 1βand IL-18 than did cells from sedentary control (SC) mice in the presence and absence of LPS, although tumor necrosis factor-αand IL-10 secretion were comparable between both groups. The mRNA levels of these cytokines increased significantly in response to LPS; similar levels were noted in macrophages from both SC and VE mice. Moreover, LPS evoked similar levels of degradation of inhibitor ofκB (IκB)αand phosphorylation of IκB kinaseβ, c-Jun N-terminal kinase, and p38 in macrophages from SC and VE mice. These results indicate that the increased IL-1βand IL-18 secretion in VE mice are regulated posttranscriptionally. On the other hand, macrophages from VE mice showed higher amounts of caspase-1 protein than did cells from SC mice. These results suggest that regular VE potentiates IL-1βand IL-18 secretion in LPS-challenged macrophages by increasing caspase-1 levels.
authors.affiliation: Department of Molecular Predictive Medicine and Sport Science, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo 181-8611, Japan.
authors.forename: Ken
authors.identifier: 0000-0002-3151-9417
authors.initials: K
authors.lastname: Shirato
authors.affiliation: Faculty of Human Sciences, Waseda University, 2-579-15 Mikajima, Tokorozawa, Saitama 359-1192, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Imaizumi
authors.affiliation: Department of Molecular Predictive Medicine and Sport Science, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo 181-8611, Japan.
authors.forename: Takuya
authors.identifier: 0000-0003-0135-8525
authors.initials: T
authors.lastname: Sakurai
authors.affiliation: Department of Molecular Predictive Medicine and Sport Science, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo 181-8611, Japan.
authors.forename: Junetsu
authors.identifier: 0000-0003-1761-9806
authors.initials: J
authors.lastname: Ogasawara
authors.affiliation: Social Medical Corporation, The Yamatokai Foundation, 1-13-12 Nangai, Higashiyamato, Tokyo 207-0014, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Ohno
authors.affiliation: Department of Molecular Predictive Medicine and Sport Science, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo 181-8611, Japan.
authors.forename: Takako
authors.identifier: 0000-0001-9222-8771
authors.initials: T
authors.lastname: Kizaki
mesh_terms: D000818:Animals; D020170:Caspase 1 / Q000378:metabolism*; D002478:Cells, Cultured; D020382:Interleukin-18 / Q000378:metabolism*; D053583:Interleukin-1beta / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010766:Phosphorylation / Q000187:drug effects; D010805:Physical Conditioning, Animal; D015843:Serpins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014764:Viral Proteins / Q000494:pharmacology
chemical_list: D020382:Interleukin-18; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D015843:Serpins; D014409:Tumor Necrosis Factor-alpha; D014764:Viral Proteins; C074641:interleukin-1beta-converting enzyme inhibitor; D020170:Caspase 1