title: Serological diagnosis of mumps: Value of the titration of specific IgG.
abstract: The aim of this study was to evaluate a cut-off point of the titration of IgG by ELISA in the diagnosis of mumps.
  
  
  
  A study was made of serum samples from 85 mumps cases (confirmed by PCR in saliva) and 2,351 controls of the general population of the Region of Madrid.
  
  
  
  The IgM detection was positive in 21 cases (sensitivity of 24.7%). The best cut-off point corresponded to IgG titres ≥4,900 (sensitivity of 64.7% and specificity of 86.1%). Among 42 patients immunised with at least one dose of measles mumps, rubella vaccine IgM was detected in 4 cases. However, the detection of IgG ≥4,900 was positive in 29 (sensitivity of 69.0%).
  
  
  
  An IgG result of ≥4.900 was almost 5 times more probable in a patient with mumps than in a non-infected patient. The detection of high titres of IgG against mumps could improve the IgM results in vaccinated people.
authors.affiliation: Laboratorio Regional de Salud Pública de la Comunidad de Madrid, Madrid, España; Programa de Prevención, Vigilancia y control de las Enfermedades Transmisibles (PREVICET), Consorcio de Investigación Biomédica de Epidemiología y Salud Pública (CIBERESP), Madrid, España. Electronic address: juan.sanz@salud.madrid.org.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Sanz
authors.affiliation: Laboratorio Regional de Salud Pública de la Comunidad de Madrid, Madrid, España.
authors.forename: Belén
authors.initials: B
authors.lastname: Ramos
authors.affiliation: Laboratorio Nacional de Referencia de Parotiditis, Centro Nacional de Microbiología (CNM), Instituto de Salud Carlos III (ISCIII), Madrid, España; Programa de Prevención, Vigilancia y control de las Enfermedades Transmisibles (PREVICET), Consorcio de Investigación Biomédica de Epidemiología y Salud Pública (CIBERESP), Madrid, España.
authors.forename: Aurora
authors.initials: A
authors.lastname: Fernández
authors.affiliation: Servicio de Epidemiología de la Comunidad de Madrid, Madrid, España.
authors.forename: Luis
authors.initials: L
authors.lastname: García-Comas
authors.affiliation: Laboratorio Nacional de Referencia de Parotiditis, Centro Nacional de Microbiología (CNM), Instituto de Salud Carlos III (ISCIII), Madrid, España; Programa de Prevención, Vigilancia y control de las Enfermedades Transmisibles (PREVICET), Consorcio de Investigación Biomédica de Epidemiología y Salud Pública (CIBERESP), Madrid, España.
authors.forename: Juan Emilio
authors.initials: JE
authors.lastname: Echevarría
authors.affiliation: Laboratorio Nacional de Referencia de Parotiditis, Centro Nacional de Microbiología (CNM), Instituto de Salud Carlos III (ISCIII), Madrid, España; Programa de Prevención, Vigilancia y control de las Enfermedades Transmisibles (PREVICET), Consorcio de Investigación Biomédica de Epidemiología y Salud Pública (CIBERESP), Madrid, España.
authors.forename: Fernando
authors.initials: F
authors.lastname: de Ory
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009107:Mumps / Q000097:blood* / Q000175:diagnosis*; D012698:Serologic Tests; D055815:Young Adult
chemical_list: D007074:Immunoglobulin G
keywords: Diagnosis; Diagnóstico; IgG; Mumps; Parotiditis; Serology; Serología