title: Identification, expression pattern and functional characterization of As-kip2 in diapause embryo restarting process of Artemia sinica.
abstract: Proper control of the cellular processes requires a variety of regulatory proteins that are involved in the cell cycle, proliferation and apoptosis. Cyclin-dependent kinase inhibitor (CKI) negatively regulates transcription and arrests the cell cycle in G1phase. KIP2 is a member of CKI family, which could inhibit proliferation by tight-binding with several cyclin-CDK complexes. During the embryonic development of the brine shrimp, Artemia sinica, KIP2 plays a key role in the cell cycle regulation, but the specific mechanisms remain unknown. Herein, the 1023bp full-length cDNA of kip2 from A. sinica was cloned. The mRNA expression patterns of As-kip2, As-carp-1 in different development stages and pattern of As-kip2 under environmental stresses were investigated. In situ hybridization of As-kip2 mRNA and immunofluorescence of As-CARP-1 protein showed no tissue or organ specificity. Furthermore, western blotting showed the expressions levels of As-KIP2, As-E2F1, As-p53, As-cyclin E, As-SODD protein, and pattern of As-KIP2 under environmental stresses. Our research revealed that As-KIP2 plays crucial role in the restarting process of diapause embryo in Artemia sinica.
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116081, China.
authors.forename: Mengchen
authors.initials: M
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116081, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Yao
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116081, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Qin
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116081, China. Electronic address: houlin@lnnu.edu.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Hou
authors.affiliation: Department of Biology, Dalian Medical University, Dalian 116044, China. Electronic address: zouxiangyang@126.com.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Zou
mesh_terms: D000818:Animals; D001156:Artemia / Q000196:embryology* / Q000235:genetics*; D003001:Cloning, Molecular; D050761:Cyclin-Dependent Kinase Inhibitor p57 / Q000235:genetics*; D004625:Embryo, Nonmammalian; D047108:Embryonic Development / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D008675:Metamorphosis, Biological / Q000235:genetics*; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D050761:Cyclin-Dependent Kinase Inhibitor p57
keywords: Artemia sinica; CARP-1; CKI; Embryonic diapause; SODD; kip2 gene