title: STV1, a ribosomal protein, binds primary microRNA transcripts to promote their interaction with the processing complex in Arabidopsis.
abstract: MicroRNAs (miRNAs) are key regulators of gene expression. They are processed from primary miRNA transcripts (pri-miRNAs), most of which are transcribed by DNA-dependent polymerase II (Pol II). miRNA levels are precisely controlled to maintain various biological processes. Here, we report that SHORT VALVE 1 (STV1), a conserved ribosomal protein, acts in miRNA biogenesis in Arabidopsis A portion of STV1 localizes in the nucleus and binds pri-miRNAs. Using pri-miR172b as a reporter, we show that STV1 binds the stem-loop flanked by a short 5' arm within pri-miRNAs. Lack of STV1 reduces the association of pri-miRNAs with their processing complex. These data suggest that STV1 promotes miRNA biogenesis through facilitating the recruitment of pri-miRNAs to their processing complex. Furthermore, we show that STV1 indirectly involves in the occupancy of Pol II at the promoters of miRNA coding genes (MIR) and influences MIR promoter activities. Based on these results, we propose that STV1 refines the accumulation of miRNAs through its combined effects on pri-miRNA processing and transcription. This study uncovers an extraribosomal function of STV1.
authors.affiliation: Center for Plant Science Innovation & School of Biological Sciences, University of Nebraska, Lincoln, NE 68588-0660.
authors.forename: Shengjun
authors.initials: S
authors.lastname: Li
authors.affiliation: Center for Plant Science Innovation & School of Biological Sciences, University of Nebraska, Lincoln, NE 68588-0660.
authors.forename: Kan
authors.initials: K
authors.lastname: Liu
authors.affiliation: Center for Plant Science Innovation & School of Biological Sciences, University of Nebraska, Lincoln, NE 68588-0660.|State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China.
authors.forename: Shuxin
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, Shanghai 200438, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, CA 92521.
authors.forename: Kestrel
authors.initials: K
authors.lastname: Rogers
authors.affiliation: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, Shanghai 200438, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Ren
authors.affiliation: Center for Plant Science Innovation & School of Biological Sciences, University of Nebraska, Lincoln, NE 68588-0660.
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Center for Plant Science Innovation & School of Biological Sciences, University of Nebraska, Lincoln, NE 68588-0660; byu3@unl.edu.
authors.forename: Bin
authors.initials: B
authors.lastname: Yu
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D012323:RNA Processing, Post-Transcriptional; D043244:Ribonuclease III / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; D035683:MicroRNAs; D012269:Ribosomal Proteins; C510495:STV1 protein, Arabidopsis; C402383:DCL1 protein, Arabidopsis; D043244:Ribonuclease III
keywords: Arabidopsis; STV1 RNA binding; miRNA biogenesis; pri-miRNAs