title: Phosphate Solubilization and Gene Expression of Phosphate-Solubilizing BacteriumBurkholderia multivoransWS-FJ9 under Different Levels of Soluble Phosphate.
abstract: Phosphate-solubilizing bacteria (PSB) have the ability to dissolve insoluble phosphate and enhance soil fertility. However, the growth and mineral phosphate solubilization of PSB could be affected by exogenous soluble phosphate and the mechanism has not been fully understood. In the present study, the growth and mineral phosphate-solubilizing characteristics of PSB strainBurkholderia multivoransWS-FJ9 were investigated at six levels of exogenous soluble phosphate (0, 0.5, 1, 5, 10, and 20 mM). The WS-FJ9 strain showed better growth at high levels of soluble phosphate. The phosphate-solubilizing activity of WS-FJ9 was reduced as the soluble phosphate concentration increased, as well as the production of pyruvic acid. Transcriptome profiling of WS-FJ9 at three levels of exogenous soluble phosphate (0, 5, and 20 mM) identified 446 differentially expressed genes, among which 44 genes were continuously up-regulated when soluble phosphate concentration was increased and 81 genes were continuously down-regulated. Some genes related to cell growth were continuously up-regulated, which would account for the better growth of WS-FJ9 at high levels of soluble phosphate. Genes involved in glucose metabolism, including glycerate kinase, 2-oxoglutarate dehydrogenase, and sugar ABC-type transporter, were continuously down-regulated, which indicates that metabolic channeling of glucose towards the phosphorylative pathway was negatively regulated by soluble phosphate. These findings represent an important first step in understanding the molecular mechanisms of soluble phosphate effects on the growth and mineral phosphate solubilization of PSB.
authors.affiliation: Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, P.R. China.
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Zeng
authors.affiliation: Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, P.R. China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Wu
authors.affiliation: Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, P.R. China.
authors.forename: Jiangchuan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, P.R. China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Ding
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000187:drug effects / Q000235:genetics; D019117:Burkholderia / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial; D020869:Gene Expression Profiling / Q000379:methods; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000235:genetics*; D063990:Gene Ontology; D005798:Genes, Bacterial / Q000235:genetics; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007655:Ketoglutarate Dehydrogenase Complex / Q000096:biosynthesis / Q000187:drug effects / Q000235:genetics; D053858:Metabolic Networks and Pathways / Q000187:drug effects / Q000235:genetics; D058977:Molecular Sequence Annotation; D010710:Phosphates / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000096:biosynthesis / Q000187:drug effects / Q000235:genetics; D019289:Pyruvic Acid / Q000378:metabolism; D012329:RNA, Bacterial / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012987:Soil; D012988:Soil Microbiology; D012995:Solubility; D059467:Transcriptome / Q000235:genetics; D015854:Up-Regulation
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D010710:Phosphates; D012329:RNA, Bacterial; D012987:Soil; D019289:Pyruvic Acid; D007655:Ketoglutarate Dehydrogenase Complex; D017853:Phosphotransferases (Alcohol Group Acceptor); C020670:glycerate kinase; D005947:Glucose
keywords: Burkholderia multivorans; Transcriptome analysis; glucose phosphorylative pathway; mineral phosphate solubilization; soluble phosphate