title: A New Human Cancer Cell Proliferation Inhibition Sesquiterpene, Dryofraterpene A, from Medicinal Plant Dryopteris fragrans (L.) Schott.
abstract: The global burden of cancer continues to increase largely with the aging and growth of the world population. The purpose of the present work was to find new anticancer molecules from a natural source. We utilized chromatographic methods to isolate compounds from medicinal plantDryopteris fragrans(L.) Schott. The structure of the new compounds was determined by spectroscopic and spectrometric data (1D NMR, 2D NMR, and EMI-MS). Their anti-proliferation effects against five human cancer cell lines including A549, MCF7, HepG2, HeLa, and PC-3 were evaluated by CCK-8 andlactate dehydrogenase (LDH) assay. A new sesquiterpene, (7S, 10S)-2,3-dihydroxy-calamenene-15-carboxylic acid methyl ester (1), and two known compounds (2and3) were isolated. The new sesquiterpene was named dryofraterpene A and significantly inhibited cancer cell proliferation without any obvious necrosis below a 10 μM concentration. In conclusion, a novel anticancer sesquiterpene together with two known compounds was isolated, which might be a promising lead compound for the treatment of cancer.
authors.affiliation: Food Science Department of XiZang Agriculture and Animal Husbandry College, Xizang 860000, China. zxzzc@163.com.
authors.forename: Zheng-Chang
authors.initials: ZC
authors.lastname: Zhong
authors.affiliation: Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Science, Heilongjiang University, Harbin 150080, China. zhaodandan@hlju.edu.cn.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Zhao
authors.affiliation: Food Science Department of XiZang Agriculture and Animal Husbandry College, Xizang 860000, China. liu304418091@126.com.
authors.forename: Zhen-Dong
authors.initials: ZD
authors.lastname: Liu
authors.affiliation: Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Science, Heilongjiang University, Harbin 150080, China. jiangshuaixbxb@foxmail.com.
authors.forename: Shuai
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Food Science Department of XiZang Agriculture and Animal Husbandry College, Xizang 860000, China. YLZhangHd@163.com.|Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Science, Heilongjiang University, Harbin 150080, China. YLZhangHd@163.com.
authors.forename: Yan-Long
authors.initials: YL
authors.lastname: Zhang
mesh_terms: D000072283:A549 Cells; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D032492:Dryopteris / Q000737:chemistry*; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D061986:MCF-7 Cells; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D012717:Sesquiterpenes / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D012717:Sesquiterpenes; C000618181:dryofraterpene A
keywords: Dryopteris fragrans (L.) Schott; cancer cell; dryofraterpene A; proliferation inhibition