title: Nosocomial transmission of carbapenem-resistant Klebsiella pneumoniae elucidated by single-nucleotide variation analysis: a case investigation.
abstract: Identifying transmission route of antimicrobial-resistant pathogen is essential for appropriate infection control strategy in healthcare facilities. We report the utility of single-nucleotide variation analysis in tracing nosocomial transmission of antimicrobial-resistant pathogens by describing a pseudo-outbreak case of carbapenem-resistant Klebsiella pneumoniae. The present case highlights that infection control strategy should encompass pathological dissection rooms, neglected but potentially highly contaminated places in hospitals.
authors.affiliation: Department of Infection Control and Prevention, Osaka University Graduate School of Medicine, Osaka, Japan.|Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.|Division of Infection Control and Prevention, Osaka University Hospital, 2-15 Yamadaoka, Suita, Osaka, 565-0871, Japan.
authors.forename: Hideharu
authors.initials: H
authors.lastname: Hagiya
authors.affiliation: Department of Microbiology and Infectious Diseases, Faculty of Medicine, Toho University School of Medicine, Tokyo, Japan.
authors.forename: Kotaro
authors.initials: K
authors.lastname: Aoki
authors.affiliation: Department of Infection Control and Prevention, Osaka University Graduate School of Medicine, Osaka, Japan. akeda@biken.osaka-u.ac.jp.|Research Institute for Microbial Diseases, Osaka University, Osaka, Japan. akeda@biken.osaka-u.ac.jp.|Division of Infection Control and Prevention, Osaka University Hospital, 2-15 Yamadaoka, Suita, Osaka, 565-0871, Japan. akeda@biken.osaka-u.ac.jp.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Akeda
authors.affiliation: Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Sakamoto
authors.affiliation: Department of Infection Control and Prevention, Osaka University Graduate School of Medicine, Osaka, Japan.|Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.|Division of Infection Control and Prevention, Osaka University Hospital, 2-15 Yamadaoka, Suita, Osaka, 565-0871, Japan.
authors.forename: Norihisa
authors.initials: N
authors.lastname: Yamamoto
authors.affiliation: Department of Infection Control and Prevention, Osaka University Graduate School of Medicine, Osaka, Japan.|Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.|Division of Infection Control and Prevention, Osaka University Hospital, 2-15 Yamadaoka, Suita, Osaka, 565-0871, Japan.
authors.forename: Hisao
authors.initials: H
authors.lastname: Yoshida
authors.affiliation: Laboratory for Clinical Investigation, Osaka University Hospital, Suita, Osaka, Japan.
authors.forename: Isao
authors.initials: I
authors.lastname: Nishi
authors.affiliation: Department of Microbiology and Infectious Diseases, Faculty of Medicine, Toho University School of Medicine, Tokyo, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Ishii
authors.affiliation: Department of Infection Control and Prevention, Osaka University Graduate School of Medicine, Osaka, Japan.|Division of Infection Control and Prevention, Osaka University Hospital, 2-15 Yamadaoka, Suita, Osaka, 565-0871, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Tomono
mesh_terms: D000369:Aged, 80 and over; D015780:Carbapenems / Q000494:pharmacology; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission*; D060005:Genotyping Techniques; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission*; D007711:Klebsiella pneumoniae / Q000145:classification* / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D008297:Male; D017720:Molecular Epidemiology; D058889:Molecular Typing; D018440:beta-Lactam Resistance
chemical_list: D015780:Carbapenems
keywords: Autopsy; Carbapenem-resistant Enterobacteriaceae; Nosocomial infection; Pathological dissection room; Whole-genome sequencing