title: Association between polymorphisms of theAPOBEC3Ggene and chronic hepatitis B viral infection and hepatitis B virus-related hepatocellular carcinoma.
abstract: OBJECTIVE
  To determine the relationship between fiveA3Ggene single nucleotide polymorphisms and the incidence of hepatitis B virus (HBV) infection and hepatocellular carcinoma (HCC).
  
  
  METHODS
  This association study was designed as a retrospective study, including 657 patients with chronic HBV infection (CHB) and 299 healthy controls. All subjects were ethnic Han Chinese. Chronic HBV-infected patients recruited between 2012 and 2015 at The First Hospital of Jilin University (Changchun) were further classified into HBV-related HCC patients (n= 287) and non-HCC patients (n= 370). Frequency matching by age and sex was performed for each group. Human genomic DNA was extracted from whole blood. Gene polymorphisms were identified using a mass spectroscopic method.
  
  
  RESULTS
  There were no significant differences between the genotype and allele frequencies of the rs7291971, rs5757465 and rs5757463A3Ggene polymorphisms, and risk of CHB and HBV-related HCC. The AG genotype and G allele for rs8177832 were significantly related to a decreased risk of CHB (OR = 0.67, 95%CI: 0.47-0.96; OR = 0.69, 95%CI: 0.50-0.95, respectively) and HCC (OR = 0.53, 95%CI: 0.34-0.84; OR = 0.58, 95%CI: 0.39-0.87, respectively). A significant relationship was found between rs2011861 computed tomography, TT genotypes and increased risk of HCC (OR = 1.69, 95%CI: 1.02-2.80; OR = 1.82, 95%CI: 1.08-3.06, respectively). Haplotype analyses showed three protective and four risk haplotypes for HCC. Also, one protective haplotype was found against CHB.
  
  
  CONCLUSIONS
  This study indicates that the A3G rs8177832 polymorphism is associated with a decreased risk of CHB infection and HCC, while the rs2011861 polymorphism is associated with an increased risk of HCC.
authors.affiliation: Xiu-Ting He, Department of Geriatrics, The First Hospital of Jilin University, Changchun 130021, Jilin Province, China.
authors.forename: Xiu-Ting
authors.initials: XT
authors.lastname: He
authors.affiliation: Xiu-Ting He, Department of Geriatrics, The First Hospital of Jilin University, Changchun 130021, Jilin Province, China.
authors.forename: Hong-Qin
authors.initials: HQ
authors.lastname: Xu
authors.affiliation: Xiu-Ting He, Department of Geriatrics, The First Hospital of Jilin University, Changchun 130021, Jilin Province, China.
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Wang
authors.affiliation: Xiu-Ting He, Department of Geriatrics, The First Hospital of Jilin University, Changchun 130021, Jilin Province, China.
authors.forename: Xiu-Shu
authors.initials: XS
authors.lastname: He
authors.affiliation: Xiu-Ting He, Department of Geriatrics, The First Hospital of Jilin University, Changchun 130021, Jilin Province, China.
authors.forename: Jun-Qi
authors.initials: JQ
authors.lastname: Niu
authors.affiliation: Xiu-Ting He, Department of Geriatrics, The First Hospital of Jilin University, Changchun 130021, Jilin Province, China.
authors.forename: Pu-Jun
authors.initials: PJ
authors.lastname: Gao
mesh_terms: D000071480:APOBEC-3G Deaminase / Q000235:genetics*; D000328:Adult; D044466:Asian People / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000453:epidemiology / Q000235:genetics* / Q000821:virology; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006515:Hepatitis B virus / Q000302:isolation & purification*; D019694:Hepatitis B, Chronic / Q000150:complications / Q000453:epidemiology / Q000235:genetics* / Q000821:virology; D006801:Humans; D015994:Incidence; D008113:Liver Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000821:virology; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012189:Retrospective Studies
chemical_list: D000071480:APOBEC-3G Deaminase; C464924:APOBEC3G protein, human
keywords: APOBEC3s; Hepatitis B viral; Hepatocellular carcinoma; Polymorphism; Progression