title: Isolation and Characterization of Synovial Mesenchymal Stem Cell Derived from Hip Joints: A Comparative Analysis with a Matched Control Knee Group.
abstract: Purpose. To determine the characteristics of MSCs from hip and compare them to MSCs from knee.Methods. Synovial tissues were obtained from both the knee and the hip joints in 8 patients who underwent both hip and knee arthroscopies on the same day. MSCs were isolated from the knee and hip synovial samples. The capacities of MSCs were compared between both groups.Results. The number of cells per unit weight at passage 0 of synovium from the knee was significantly higher than that from the hip (P< 0.05). While it was possible to observe the growth of colonies in all the knee synovial fluid samples, it was impossible to culture cells from any of the hip samples. In adipogenesis experiments, the frequency of Oil Red-O-positive colonies and the gene expression of adipsin were significantly higher in knee than in hip. In osteogenesis experiments, the expression of COL1A1 and ALPP was significantly less in the knee synovium than in the hip synovium.Conclusions. MSCs obtained from hip joint have self-renewal and multilineage differentiation potentials. However, in matched donors, adipogenesis and osteogenesis potentials of MSCs from the knees are superior to those from the hips. Knee synovium may be a better source of MSC for potential use in hip diseases.
authors.affiliation: Department of Orthopaedic Surgery, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: Akihisa
authors.initials: A
authors.lastname: Hatakeyama
authors.affiliation: Department of Orthopaedic Surgery, Wakamatsu Hospital, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: Soshi
authors.identifier: 0000-0002-5975-8276
authors.initials: S
authors.lastname: Uchida
authors.affiliation: Department of Orthopaedic Surgery, Wakamatsu Hospital, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Utsunomiya
authors.affiliation: Department of Orthopaedic Surgery, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Tsukamoto
authors.affiliation: Department of Orthopaedic Surgery, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Nakashima
authors.affiliation: Department of Orthopaedic Surgery, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: Eiichiro
authors.initials: E
authors.lastname: Nakamura
authors.affiliation: Department of Orthopaedic Surgery, Washington University School of Medicine, St. Louis, MO, USA.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Pascual-Garrido
authors.affiliation: Department of Cartilage Regeneration, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Sekiya
authors.affiliation: Department of Orthopaedic Surgery, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: Akinori
authors.initials: A
authors.lastname: Sakai