title: Feasibility of ultra-performance liquid chromatography-ion mobility-time-of-flight mass spectrometry in analyzing oxysterols.
abstract: Oxysterols are oxygenated cholesterols that are important in many cell functions and they may also be indicative of certain diseases. The purpose of this work was to study the feasibility of ultra-performance liquid chromatography-ion mobility-time-of-flight mass spectrometry (UPLC-IM-TOFMS) using traveling wave cell in analyzing oxysterols and especially their isomers in biological samples. Oxysterols were analyzed as their p-toluenesulfonyl isocyanate derivatives, which improved the separation of isomeric oxysterols by ion mobility and ionization efficiency in the electrospray ionization step. The UPLC-IM-TOFMS method was shown to be fast and to provide good quantitative performance. The feasibility of the method was demonstrated in the analyses of oxysterols in fibroblast cell samples.
authors.affiliation: Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, P.O. Box 56, FI-00014, Finland. Electronic address: petri.kylli@helsinki.fi.
authors.forename: Petri
authors.initials: P
authors.lastname: Kylli
authors.affiliation: Division of Analytical Biosciences, Leiden Academic Centre for Drug Research, Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands; Netherlands Metabolomics Centre, Leiden University, P.O. Box 9502, 22300 RA, Leiden, The Netherlands. Electronic address: hankemeier@lacdr.leidenuniv.nl.
authors.forename: Thomas
authors.initials: T
authors.lastname: Hankemeier
authors.affiliation: Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, P.O. Box 56, FI-00014, Finland. Electronic address: risto.kostiainen@helsinki.fi.
authors.forename: Risto
authors.initials: R
authors.lastname: Kostiainen
mesh_terms: D002853:Chromatography, Liquid; D005347:Fibroblasts / Q000737:chemistry; D006801:Humans; D007536:Isomerism; D013058:Mass Spectrometry; D000072376:Oxysterols / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000072376:Oxysterols
keywords: Derivatization; Fibroblast cell samples; Liquid-chromatography-ion-mobility-mass-spectrometry,oxysterol; Oxysterol isomers