title: New parameters in assessment of human donor corneal stroma.
abstract: OBJECTIVE
  To provide quantitative parameters for assessment of human donor corneal stroma by imaging stromal features of diseased and normal human corneas with full-field optical coherence microscopy (FFOCM), using confocal microscopy (CM) and histology as reference techniques.
  
  
  METHODS
  Bowman's layer (BL) thickness and keratocyte density were assessed ex vivo in 23 human donor corneas and 27 human pathological corneas (keratoconus and other corneal disorders) with FFOCM, CM and histology. Stromal backscattering was assessed with FFOCM. Additionally, 10 normal human corneas were assessed in vivo with CM.
  
  
  RESULTS
  In FFOCM, the logarithm of the normalized stromal reflectivity was a linear function of stromal depth (R2= 0.95) in human donor corneas. Compared with keratoconus corneas, human donor corneas featured higher BL thickness (p = 0.0014) with lower coefficient of variation (BL-COV; p = 0.0002), and linear logarithmic stromal reflectivity with depth (higher R2, p = 0.0001). Compared with other corneal disorders, human donor corneas featured lower BL-COV (p = 0.012) and higher R2(p = 0.0001). Using the 95% confidence limits of the human donor cornea group, BL thickness < 6.5 μm (sensitivity, 57%; specificity, 100%), BL-COV > 18.6% (79%; 100%) and R2< 0.94 (93%; 71%) were revealed as indictors of abnormal cornea. In CM, keratocyte density decreased with stromal depth (r = -0.56). The mean overall keratocyte density (cells/mm2) was 205 in human donor corneas, 244 in keratoconus, 176 in other corneal disorders and 386 in normal corneas.
  
  
  CONCLUSIONS
  Full-field optical coherence microscopy (FFOCM) provides precise and reliable parameters for non-invasive assessment of human donor corneal stroma during storage, enabling detection of stromal disorders that could impair the results of keratoplasty.
authors.affiliation: Quinze-Vingts National Ophthalmology Hospital, Paris VI University, Paris, France.|Vision Institute/CIC 1423, UMR_S 968/INSERM, U968/CHNO/CNRS, UMR_7210, UPMC University Paris 06, Paris, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Borderie
authors.affiliation: Quinze-Vingts National Ophthalmology Hospital, Paris VI University, Paris, France.|Vision Institute/CIC 1423, UMR_S 968/INSERM, U968/CHNO/CNRS, UMR_7210, UPMC University Paris 06, Paris, France.
authors.forename: Kate
authors.initials: K
authors.lastname: Grieve
authors.affiliation: Quinze-Vingts National Ophthalmology Hospital, Paris VI University, Paris, France.|Vision Institute/CIC 1423, UMR_S 968/INSERM, U968/CHNO/CNRS, UMR_7210, UPMC University Paris 06, Paris, France.|Laboratory of Ophthalmic Instrument Development, The Wilmer Eye Institute, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: Kristina
authors.initials: K
authors.lastname: Irsch
authors.affiliation: Quinze-Vingts National Ophthalmology Hospital, Paris VI University, Paris, France.|Vision Institute/CIC 1423, UMR_S 968/INSERM, U968/CHNO/CNRS, UMR_7210, UPMC University Paris 06, Paris, France.
authors.forename: Djida
authors.initials: D
authors.lastname: Ghoubay
authors.affiliation: Quinze-Vingts National Ophthalmology Hospital, Paris VI University, Paris, France.|Vision Institute/CIC 1423, UMR_S 968/INSERM, U968/CHNO/CNRS, UMR_7210, UPMC University Paris 06, Paris, France.
authors.forename: Cristina
authors.initials: C
authors.lastname: Georgeon
authors.affiliation: Tissue Bank, French Blood Establishment, Paris, Ile-de-France, France.
authors.forename: Celine
authors.initials: C
authors.lastname: De Sousa
authors.affiliation: Quinze-Vingts National Ophthalmology Hospital, Paris VI University, Paris, France.|Vision Institute/CIC 1423, UMR_S 968/INSERM, U968/CHNO/CNRS, UMR_7210, UPMC University Paris 06, Paris, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Laroche
authors.affiliation: Quinze-Vingts National Ophthalmology Hospital, Paris VI University, Paris, France.|Vision Institute/CIC 1423, UMR_S 968/INSERM, U968/CHNO/CNRS, UMR_7210, UPMC University Paris 06, Paris, France.
authors.forename: Vincent M
authors.initials: VM
authors.lastname: Borderie
mesh_terms: D000328:Adult; D000368:Aged; D003316:Corneal Diseases / Q000601:surgery*; D003319:Corneal Stroma / Q000166:cytology* / Q000637:transplantation; D016039:Corneal Transplantation; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D018613:Microscopy, Confocal / Q000379:methods*; D008875:Middle Aged; D011446:Prospective Studies; D014019:Tissue Donors; D041623:Tomography, Optical Coherence / Q000379:methods*
keywords: cornea; donor tissue; keratocyte; optical coherence tomography; stroma