title: Effect of climate change on phytochemical diversity, total phenolic content and in vitro antioxidant activity of Aloe vera (L.) Burm.f.
abstract: BACKGROUND
  The aim of the present study was to analyse the effect of climate change on phytochemicals, total phenolic content (TPC) and antioxidant potential of methanolic extracts of Aloe vera collected from different climatic zones of the India.
  
  
  METHODS
  Crude methanolic extracts of A. vera from the different states of India were screened for presence of various phytochemicals, total phenolic content and in vitro antioxidant activity. Total phenolic content was tested by Folin-Ciocalteau reagent based assay whilst DPPH free radical scavenging assay, metal chelating assay, hydrogen peroxide scavenging assay, reducing power assay and β carotene-linoleic assay were used to assess the antioxidant potential of A. vera methanolic leaf extracts.
  
  
  RESULTS
  Alkaloids, phenols, flavonoids, saponins, and terpenes were the main phytochemicals presents in all accessions. A significant positive correlation was found between TPC and antioxidant activity of different accessions. Extracts of highland and semi-arid zones possessed maximum antioxidant potential. Accessions from tropical zones showed the least antioxidant activity in all assays.
  
  
  CONCLUSIONS
  It could be concluded that different agro-climatic conditions have effects on the phytochemicals, total phenolic content (TPC) and antioxidant potential of the A. vera plant. The results reveal that A. vera can be a potential source of novel natural antioxidant compounds.
authors.affiliation: Department of Genetics, M.D. University, Rohtak, Haryana, 124001, India.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Department of Genetics, M.D. University, Rohtak, Haryana, 124001, India.
authors.forename: Amita
authors.initials: A
authors.lastname: Yadav
authors.affiliation: Department of Genetics, M.D. University, Rohtak, Haryana, 124001, India.
authors.forename: Manila
authors.initials: M
authors.lastname: Yadav
authors.affiliation: Department of Genetics, M.D. University, Rohtak, Haryana, 124001, India. yadav1964@rediffmail.com.
authors.forename: Jaya Parkash
authors.identifier: http://orcid.org/0000-0002-3500-6811
authors.initials: JP
authors.lastname: Yadav
mesh_terms: D000504:Aloe / Q000737:chemistry*; D000975:Antioxidants / Q000032:analysis*; D001713:Biphenyl Compounds / Q000737:chemistry; D002614:Chelating Agents / Q000737:chemistry; D057231:Climate Change; D016166:Free Radical Scavengers / Q000737:chemistry; D005843:Geography; D006861:Hydrogen Peroxide / Q000737:chemistry; D010636:Phenols / Q000032:analysis*; D064209:Phytochemicals / Q000032:analysis*; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D011891:Rain; D013696:Temperature
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D002614:Chelating Agents; D016166:Free Radical Scavengers; D010636:Phenols; D064209:Phytochemicals; D010851:Picrates; D010936:Plant Extracts; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Agro-climatic zones; Aloe vera; Antioxidant; Methanol; Phytochemicals; Total phenolic content (TPC)