title: Single nucleotide polymorphisms, haplotype association and tumour expression of the vascular endothelial growth factor (VEGF) gene with lung carcinoma.
abstract: VEGF contains several polymorphic sites known to influence its expression. We examined the possible association between+405(-634)C>G,+936C>T,-2578C>A and lung cancer in 199 Kashmiri patients and 401 healthy controls. VEGF+405CG,+936CT+TT and-2578CA genotypes were significantly associated with lung cancer risk compared to VEGF+405CC,+936CC and-2578AA+CC genotypes [OR=0.07 (0.04-0.13), P<0.0001, OR=0.36 (0.25-0.52), P<0.0001 and 0.08 (0.05-0.13), P<0.0001]. Haplotype analysis revealed that CGA and TGA haplotypes of VEGF gene conveys the risk for lung cancer [OR=0.18 (0.10-0.33), P<0.0001 and 0.07 (0.03-0.13), P<0.0001]. VEGF expression revealed non-significant association with the genotypes of the three SNPs. In conclusion, the SNPs examined appear to influence lung cancer susceptibility while as genotypes of the SNPs don't appear to have significant association with VEGF mRNA expression in lung tumours.
authors.affiliation: Department of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, 190011, Kashmir, India. Electronic address: drniyaznaik@gmail.com.
authors.forename: Niyaz A
authors.initials: NA
authors.lastname: Naykoo
authors.affiliation: Department of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, 190011, Kashmir, India.
authors.lastname: Dil-Afroze
authors.affiliation: Department of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, 190011, Kashmir, India.
authors.forename: Roohi
authors.initials: R
authors.lastname: Rasool
authors.affiliation: Department of General Medicine, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, 190011, Kashmir, India.
authors.forename: Sonaullah
authors.initials: S
authors.lastname: Shah
authors.affiliation: Department of Cardio Vascular Thoracic Surgery, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, 190011, Kashmir, India.
authors.forename: A G
authors.initials: AG
authors.lastname: Ahangar
authors.affiliation: Department of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, 190011, Kashmir, India.
authors.forename: Imtiyaz A
authors.initials: IA
authors.lastname: Bhat
authors.affiliation: Advanced Centre for Human Genetics, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, 190011, Kashmir, India.
authors.forename: Iqbal
authors.initials: I
authors.lastname: Qasim
authors.affiliation: Islamic University of Science and Technology Awantipora Pulwama J&K, 192122, India.
authors.forename: Mushtaq A
authors.initials: MA
authors.lastname: Siddiqi
authors.affiliation: Department of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, 190011, Kashmir, India. Electronic address: zaffaramin@gmail.com.
authors.forename: Zafar A
authors.initials: ZA
authors.lastname: Shah
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A
keywords: Expression; Haplotype; Lung; SNP; VEGF