title: High Efficiency Binding Aptamers for a Wide Range of Bacterial Sepsis Agents.
abstract: Sepsis is a major health problem worldwide, with an extremely high rate of morbidity and mortality, partly due to delayed diagnosis during early disease. Currently, sepsis diagnosis requires bacterial culturing of blood samples over several days, whereas PCR-based molecular diagnosis methods are faster but lack sensitivity. The use of biosensors containing nucleic acid aptamers that bind targets with high affinity and specificity could accelerate sepsis diagnosis. Previously, we used the systematic evolution of ligands by exponential enrichment technique to develop the aptamers Antibac1 and Antibac2, targeting the ubiquitous bacterial peptidoglycan. Here, we show that these aptamers bind to four gram-positive and seven gram-negative bacterial sepsis agents with high binding efficiency. Thus, these aptamers could be used in combination as biological recognition elements in the development of biosensors that are an alternative to rapid bacteria detection, since they could provide culture and amplification-free tests for rapid clinical sepsis diagnosis.
authors.affiliation: Instituto de Biologia Molecular do Paraná, Department of Research and Development, Curitiba, Paraná, Brazil.
authors.forename: Ana Cláudia
authors.initials: AC
authors.lastname: Graziani
authors.affiliation: Instituto de Biologia Molecular do Paraná, Department of Research and Development, Curitiba, Paraná, Brazil.
authors.forename: Maria Isabel
authors.initials: MI
authors.lastname: Stets
authors.affiliation: Instituto de Biologia Molecular do Paraná, Department of Research and Development, Curitiba, Paraná, Brazil.
authors.forename: Ana Luisa Kalb
authors.initials: ALK
authors.lastname: Lopes
authors.affiliation: Instituto de Biologia Molecular do Paraná, Department of Research and Development, Curitiba, Paraná, Brazil.
authors.forename: Pedro Henrique Caires
authors.initials: PHC
authors.lastname: Schluga
authors.affiliation: Instituto de Biologia Molecular do Paraná, Department of Research and Development, Curitiba, Paraná, Brazil.
authors.forename: Soledad
authors.initials: S
authors.lastname: Marton
authors.affiliation: Centro de Desenvolvimento da Tecnologia Nuclear (CDTN), Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Ieda Ferreira
authors.initials: IF
authors.lastname: Mendes
authors.affiliation: Centro de Desenvolvimento da Tecnologia Nuclear (CDTN), Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Antero Silva Ribeiro de
authors.initials: ASR
authors.lastname: Andrade
authors.affiliation: Instituto de Biologia Molecular do Paraná, Department of Research and Development, Curitiba, Paraná, Brazil.
authors.forename: Marco Aurélio
authors.initials: MA
authors.lastname: Krieger
authors.affiliation: Instituto de Biologia Molecular do Paraná, Department of Research and Development, Curitiba, Paraná, Brazil.
authors.forename: Josiane
authors.initials: J
authors.lastname: Cardoso
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000235:genetics; D001419:Bacteria / Q000235:genetics* / Q000472:pathogenicity; D015374:Biosensing Techniques / Q000379:methods; D018929:Cell Culture Techniques / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008024:Ligands; D025202:Molecular Diagnostic Techniques / Q000379:methods; D010457:Peptidoglycan / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D052156:SELEX Aptamer Technique / Q000379:methods*; D012680:Sensitivity and Specificity; D018805:Sepsis / Q000175:diagnosis* / Q000382:microbiology*
chemical_list: D052157:Aptamers, Nucleotide; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D008024:Ligands; D010457:Peptidoglycan
keywords: Anti-peptidoglycan aptamers; bacterial sepsis agents; biosensor; qPCR assays