title: The association of different auto-antibodies against ovarian tissues and gonadotropins and poor ovarian response in intracytoplasmic sperm injection cycles.
abstract: This study aimed to assess the possible association between ovarian auto-antibodies and poor ovarian response to controlled ovarian hyperstimulation (COH) in patients undergoing intracytoplasmic sperm injection (ICSI) cycles. In total, 42 poor responders and 43 male factor subjects were enrolled in the study and underwent either a standard long gonadotropin-releasing hormone (GnRH) agonist or antagonist protocol. Anti-ovarian, anti-oocyte, anti-zona pellucida (anti-ZP) and anti-gonadotropin antibodies in their sera and follicular fluid (FF) were measured by an enzyme-linked immunosorbent assay technique (ELISA). The mean follicular fluid anti-oocyte antibody [ratio of optical density (OD)sample/ODControl] was significantly higher in poor responders compared to the normal group (2.40 ± 1.55 versus 1.72 ± 0.71, p = 0.012). The linear regression analysis showed an inverse correlation between FF anti-oocyte antibody concentrations and the number of: (i) retrieved oocytes (B = -1.212, r = -0.235, p = 0.030); (ii) mature oocytes (B = -1.042, r = -0.234, p = 0.031); (iii) embryos available (B = -0.713, r = -0.228, p = 0.036); and (iv) good quality embryos (B = -0.369, r = -0.229, p = 0.035). However, there were no significant differences between two groups in terms of FF and serum anti-ovarian, anti-gonadotropins and anti-ZP antibodies. The Pearson correlation analysis on 85 infertile patients showed a positive correlation between age and the levels of FF anti-oocyte antibody (r = 0.276, p = 0.010). This study demonstrated that FF anti-oocyte antibody could be associated with poor response to COH in ICSI cycles.
authors.affiliation: a Department of Endocrinology and Female Infertility , Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine , Tehran , Iran.
authors.forename: Ladan
authors.initials: L
authors.lastname: Mohammadi Yeganeh
authors.affiliation: a Department of Endocrinology and Female Infertility , Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine , Tehran , Iran.|b Department of Gynecology and Obstetrics , Roointan Arash Woman's Health Research and Educational Hospital, Tehran University of Medical Sciences , Tehran , Iran.|c Vali-e-Asr Reproductive Health Research Center, Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Ashraf
authors.initials: A
authors.lastname: Moini
authors.affiliation: a Department of Endocrinology and Female Infertility , Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine , Tehran , Iran.
authors.forename: Mandana
authors.initials: M
authors.lastname: Hemmat
authors.affiliation: d Department of Andrology , Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine , Tehran , Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Salman Yazdi
authors.affiliation: e Department of Epidemiology and Reproductive Health , Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR , Tehran , Iran.
authors.forename: Narges
authors.initials: N
authors.lastname: Bagheri Lankarani
authors.affiliation: a Department of Endocrinology and Female Infertility , Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine , Tehran , Iran.
authors.forename: Shabnam
authors.initials: S
authors.lastname: Khodabakhshi
authors.affiliation: f Department of Embryology , Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine , Tehran , Iran.
authors.forename: Arash
authors.initials: A
authors.lastname: Behbahanian
mesh_terms: D000328:Adult; D001323:Autoantibodies; D005260:Female; D006062:Gonadotropins / Q000276:immunology*; D006801:Humans; D007247:Infertility, Female; D008297:Male; D010053:Ovary / Q000276:immunology* / Q000502:physiology*; D010062:Ovulation Induction; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic; D055815:Young Adult
chemical_list: D001323:Autoantibodies; D006062:Gonadotropins
keywords: Anti-ovarian antibodies; in vitro fertilization; intracytoplasmic sperm injection; poor ovarian response