title: Molecular identification of trypanosome species in trypanotolerant cattle from the south of Gabon.
abstract: The aim of this study was to provide information on trypanosome species infecting trypanotolerant cattle from southern Gabon. The study was conducted on 224 trypanotolerant cattle from three regions located in southern Gabon, using ITS1 primer-based PCR. Seventy-two (32%) N'dama cattle were found polymerase chain reaction (PCR) positive with trypanosomes. The overall prevalence of trypanosomosis was 57% (63/110), 4% (4/100), and 36% (5/14) in the Gala section of the Nyanga ranch, the Miyama ranch, and Ossiele, respectively. Trypanosoma congolense and Trypanosoma vivax were identified. In Gala section and Ossiele, T. congolense and T. vivax were found. In the Miyama ranch, only T. vivax was identified. Mixed infections were also found. The forest (9%) and savannah (63%) subgroups of T. congolense were identified. The presence of the two subgroups was detected in 16 out of 56 cattle (29%). T. congolense and T. vivax would appear to be the main agents responsible for bovine trypanosomosis in southern Gabon. Although trypanotolerant, N'dama cattle may serve as a reservoir, and this should be further studied. On the other hand, these trypanotolerant cattle can be reared in such tsetse infested areas, which gives them an advantage compared to other trypanosensitive breeds, and this shows that they represent a key factor in biodiversity which has to be promoted.
authors.affiliation: Centre International de Recherches Médicales de Franceville, BP 769 Franceville, Gabon - Université des Sciences et Techniques de Masuku (USTM), Institut National Supérieur d'Agronomie et de Biotechnologies (INSAB), BP 913 Franceville, Gabon.
authors.forename: Gaël Darren
authors.initials: GD
authors.lastname: Maganga
authors.affiliation: Institut de Recherche en Écologie Tropicale (IRET-CENAREST), BP 13354 Libreville, Gabon - Université des Sciences et Techniques de Masuku (USTM), Institut National Supérieur d'Agronomie et de Biotechnologies (INSAB), BP 913 Franceville, Gabon.
authors.forename: Jacques-François
authors.initials: JF
authors.lastname: Mavoungou
authors.affiliation: Centre International de Recherches Médicales de Franceville, BP 769 Franceville, Gabon.
authors.forename: Nadine
authors.initials: N
authors.lastname: N'dilimabaka
authors.affiliation: Centre International de Recherches Médicales de Franceville, BP 769 Franceville, Gabon.
authors.forename: Ivan Cyr
authors.initials: IC
authors.lastname: Moussadji Kinga
authors.affiliation: Centre International de Recherches Médicales de Franceville, BP 769 Franceville, Gabon.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Mvé-Ondo
authors.affiliation: Centre International de Recherches Médicales de Franceville, BP 769 Franceville, Gabon.
authors.forename: Illich Manfred
authors.initials: IM
authors.lastname: Mombo
authors.affiliation: Centre International de Recherches Médicales de Franceville, BP 769 Franceville, Gabon.
authors.forename: Barthélémy
authors.initials: B
authors.lastname: Ngoubangoye
authors.affiliation: SIAT-Gabon Group, BP 3928 Libreville, Gabon.
authors.forename: Brieuc
authors.initials: B
authors.lastname: Cossic
authors.affiliation: Université des Sciences et Techniques de Masuku (USTM), Institut National Supérieur d'Agronomie et de Biotechnologies (INSAB), BP 913 Franceville, Gabon.
authors.forename: Clency Sylde
authors.initials: CS
authors.lastname: Mikala Okouyi
authors.affiliation: Université des Sciences et Techniques de Masuku (USTM), Institut National Supérieur d'Agronomie et de Biotechnologies (INSAB), BP 913 Franceville, Gabon.
authors.forename: Alain
authors.initials: A
authors.lastname: Souza
authors.affiliation: Centre International de Recherches Médicales de Franceville, BP 769 Franceville, Gabon.
authors.forename: Eric Maurice
authors.initials: EM
authors.lastname: Leroy
authors.affiliation: Université des Sciences et Techniques de Masuku (USTM), Institut National Supérieur d'Agronomie et de Biotechnologies (INSAB), BP 913 Franceville, Gabon.
authors.forename: Brice
authors.initials: B
authors.lastname: Kumulungui
authors.affiliation: Centre International de Recherches Médicales de Franceville, BP 769 Franceville, Gabon.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Ollomo
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D016000:Cluster Analysis; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005681:Gabon; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012307:Risk Factors; D014348:Trypanosoma congolense / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D016689:Trypanosoma vivax / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D014354:Trypanosomiasis, Bovine / Q000453:epidemiology / Q000276:immunology / Q000469:parasitology*
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal