title: The involvement of spinal release of histamine on nociceptive behaviors induced by intrathecally administered spermine.
abstract: The involvement of spinal release of histamine on nociceptive behaviors induced by spermine was examined in mice. Intrathecal spermine produced dose-dependent nociceptive behaviors, consisting of scratching, biting and licking. The nociceptive behaviors induced by spermine at 0.02 amol and 10 pmol were markedly suppressed by i.t. pretreatment with antiserum against histamine and were abolished in histidine decarboxylase-deficient mice. In histamine H1receptor-deficient mice, the nociceptive behaviors induced by spermine were completely abolished after treatment with 0.02 amol of spermine and significantly suppressed after treatment with 10 pmol of spermine. The i.t. pretreatment with takykinin NK1receptor antagonists eliminated the nociceptive behaviors induced by 0.02 amol of spermine, but did not affect the nociceptive behaviors induced by 10 pmol of spermine. On the other hand, the nociceptive behaviors induced by spermine at both 0.02 amol and 10 pmol were suppressed by i.t. pretreatment with antagonists for the NMDA receptor polyamine-binding site. The present results suggest that the nociceptive behaviors induced by i.t. administration of spermine are mediated through the spinal release of histamine and are elicited via activation of NMDA receptors.
authors.affiliation: Department of Physiology and Anatomy, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Mizoguchi
authors.affiliation: Department of Physiology and Anatomy, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan.
authors.forename: Chizuko
authors.initials: C
authors.lastname: Watanabe
authors.affiliation: Department of Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan.
authors.forename: Takafumi
authors.initials: T
authors.lastname: Hayashi
authors.affiliation: Department of Physiology and Anatomy, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Iwata
authors.affiliation: Department of Pharmaceutical Biosciences, Division of Biological Research on Drug Dependence, Uppsala University, Husargatan 3, Uppsala 751 24, Sweden.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Watanabe
authors.affiliation: Center for Experiential Pharmacy Practice, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
authors.forename: Soh
authors.initials: S
authors.lastname: Katsuyama
authors.affiliation: First Department of Pharmacology, Daiichi College of Pharmaceutical Sciences, 22-1 Tamagawa-cho, Minami-ku, Fukuoka 815-8511, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Hamamura
authors.affiliation: First Department of Pharmacology, Daiichi College of Pharmaceutical Sciences, 22-1 Tamagawa-cho, Minami-ku, Fukuoka 815-8511, Japan.
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Sakurada
authors.affiliation: Department of Quantum Science and Energy Engineering, Graduate School of Engineering, Tohoku University, 6-6-01-2 Aobayama, Aoba-ku, Sendai, 980-8579, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ohtsu
authors.affiliation: Department of Pharmacology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Yanai
authors.affiliation: Department of Physiology and Anatomy, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan. Electronic address: s-sakura@tohoku-mpu.ac.jp.
authors.forename: Shinobu
authors.initials: S
authors.lastname: Sakurada
mesh_terms: D000818:Animals; D006636:Histamine Release / Q000187:drug effects*; D008297:Male; D051379:Mice; D059225:Nociception / Q000187:drug effects*; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D018039:Receptors, Tachykinin / Q000378:metabolism; D013096:Spermine / Q000008:administration & dosage* / Q000494:pharmacology*; D013115:Spinal Canal; D013116:Spinal Cord / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D016194:Receptors, N-Methyl-D-Aspartate; D018039:Receptors, Tachykinin; D013096:Spermine
keywords: Histamine; NMDA receptor; Nociception; Release; Spermine; Spinal cord