title: Neuroprotective Effects of Bioavailable Polyphenol-Derived Metabolites against Oxidative Stress-Induced Cytotoxicity in Human Neuroblastoma SH-SY5Y Cells.
abstract: Oxidative stress is involved in cell death in neurodegenerative diseases. Dietary polyphenols can exert health benefits, but their direct effects on neuronal cells are debatable because most phenolics are metabolized and do not reach the brain as they occur in the dietary sources. Herein, we evaluate the effects of a panel of bioavailable polyphenols and derived metabolites at physiologically relevant conditions against H2O2-induced apoptosis in human neuroblastoma SH-SY5Y cells. Among the 19 metabolites tested, 3,4-dihydroxyphenylpropionic acid, 3,4-dihydroxyphenylacetic acid, gallic acid, ellagic acid, and urolithins prevented neuronal apoptosis via attenuation of ROS levels, increased REDOX activity, and decreased oxidative stress-induced apoptosis by preventing the caspase-3 activation via the mitochondrial apoptotic pathway in SH-SY5Y cells. This suggests that dietary sources containing the polyphenol precursors of these molecules such as cocoa, berries, walnuts, and tea could be potential functional foods to reduce oxidative stress associated with the onset and progress of neurodegenerative diseases.
authors.affiliation: Research Group on Quality, Safety, and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS-CSIC , P.O. Box 164, 30100 Campus de Espinardo, Murcia, Spain.
authors.forename: Antonio
authors.identifier: http://orcid.org/0000-0002-3407-0678
authors.initials: A
authors.lastname: González-Sarrías
authors.affiliation: Research Group on Quality, Safety, and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS-CSIC , P.O. Box 164, 30100 Campus de Espinardo, Murcia, Spain.
authors.forename: María Ángeles
authors.initials: MÁ
authors.lastname: Núñez-Sánchez
authors.affiliation: Research Group on Quality, Safety, and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS-CSIC , P.O. Box 164, 30100 Campus de Espinardo, Murcia, Spain.
authors.forename: Francisco A
authors.identifier: http://orcid.org/0000-0002-0790-1739
authors.initials: FA
authors.lastname: Tomás-Barberán
authors.affiliation: Research Group on Quality, Safety, and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS-CSIC , P.O. Box 164, 30100 Campus de Espinardo, Murcia, Spain.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Espín
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D006801:Humans; D015394:Molecular Structure; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D059808:Polyphenols / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D018696:Neuroprotective Agents; D059808:Polyphenols; D053148:Caspase 3
keywords: ellagic acid; gallic acid; neuroprotective effects; phenolic acids; polyphenol; urolithins