title: Preparation and68Ga-radiolabeling of porous zirconia nanoparticle platform for PET/CT-imaging guided drug delivery.
abstract: This paper describes the preparation of gallium-68 (68Ga) isotope labeled porous zirconia (ZrO2) nanoparticle (NP) platform of nearly 100nm diameter and its first pharmacokinetic and biodistribution evaluation accomplished with a microPET/CT (μPet/CT) imaging system. Objectives of the investigations were to provide a nanoparticle platform which can be suitable for specific delivery of various therapeutic drugs using surface attached specific molecules as triggering agents, and at the same time, suitable for positron emission tomography (PET) tracing of the prospective drug delivery process. Radiolabeling was accomplished using DOTA bifunctional chelator. DOTA was successfully adsorbed onto the surface of nanoparticles, while the68Ga-radiolabeling method proved to be simple and effective. In the course of biodistribution studies, the68Ga-labeled DOTA-ZrNPs showed proper radiolabeling stability in their original suspension and in blood serum. μPet/CT imaging studies confirmed a RES-biodistribution profile indicating stable nano-sized labeled particles in vivo. Results proved that the new method offers the opportunity to examine further specifically targeted and drug payload carrier variants of zirconia NP systems using PET/CT imaging.
authors.affiliation: Department of Nuclear Medicine, Hannover Medical School, Carl-Neuberg Str 1, 30627 Hannover, Germany. Electronic address: polyak.andras@mh-hannover.de.
authors.forename: Andras
authors.initials: A
authors.lastname: Polyak
authors.affiliation: MTA-SE Molecular Biophysics Research Group, Semmelweis University, Tűzoltó Str 37-47, H-1094 Budapest, Hungary; Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar Tudósok Blvd 2, H-1117 Budapest, Hungary.
authors.forename: Lívia
authors.initials: L
authors.lastname: Naszalyi Nagy
authors.affiliation: Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar Tudósok Blvd 2, H-1117 Budapest, Hungary.
authors.forename: Judith
authors.initials: J
authors.lastname: Mihaly
authors.affiliation: Department of Nuclear Medicine, Hannover Medical School, Carl-Neuberg Str 1, 30627 Hannover, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Görres
authors.affiliation: Department of Nuclear Medicine, Hannover Medical School, Carl-Neuberg Str 1, 30627 Hannover, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Wittneben
authors.affiliation: Department of Nuclear Medicine, Hannover Medical School, Carl-Neuberg Str 1, 30627 Hannover, Germany; Institute for Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine, Hannover Bünteweg 2, 30559 Hannover Germany.
authors.forename: Ina
authors.initials: I
authors.lastname: Leiter
authors.affiliation: Department of Nuclear Medicine, Hannover Medical School, Carl-Neuberg Str 1, 30627 Hannover, Germany.
authors.forename: Jens P
authors.initials: JP
authors.lastname: Bankstahl
authors.affiliation: Nanotechnology Department, Laser Zentrum Hannover e.V., Hollerithallee 8, D-30419 Hannover, Germany.
authors.forename: Laszlo
authors.initials: L
authors.lastname: Sajti
authors.affiliation: MTA-SE Molecular Biophysics Research Group, Semmelweis University, Tűzoltó Str 37-47, H-1094 Budapest, Hungary.
authors.forename: Miklós
authors.initials: M
authors.lastname: Kellermayer
authors.affiliation: MTA-SE Molecular Biophysics Research Group, Semmelweis University, Tűzoltó Str 37-47, H-1094 Budapest, Hungary; Nanochemistry Research Group, Semmelweis University, Nagyvárad Sqr 4, 1089 Budapest, Hungary.
authors.forename: Miklós
authors.initials: M
authors.lastname: Zrínyi
authors.affiliation: Department of Nuclear Medicine, Hannover Medical School, Carl-Neuberg Str 1, 30627 Hannover, Germany.
authors.forename: Tobias L
authors.initials: TL
authors.lastname: Ross
mesh_terms: D002614:Chelating Agents / Q000737:chemistry / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods; D005710:Gallium Radioisotopes / Q000737:chemistry* / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism; D000072078:Positron Emission Tomography Computed Tomography / Q000379:methods; D049268:Positron-Emission Tomography / Q000379:methods; D019275:Radiopharmaceuticals / Q000737:chemistry* / Q000378:metabolism*; D014018:Tissue Distribution / Q000187:drug effects; D015040:Zirconium / Q000737:chemistry* / Q000378:metabolism
chemical_list: D002614:Chelating Agents; D005710:Gallium Radioisotopes; D019275:Radiopharmaceuticals; D015040:Zirconium; C028541:zirconium oxide
keywords: 68Ga; DOTA; Imaging guided therapy; Nanoparticles; PET; Theranostics