title: Osteogenesis Catalyzed by Titanium-Supported Silver Nanoparticles.
abstract: Silver nanoparticles (Ag NPs) were widely explored for antimicrobial applications, whereas the translation into drugs and implantable antibacterial devices provoked serious concerns about their potential cytotoxicity. Herein, Ag NPs with diameters ranging from 4 to 19 nm were in situ fabricated and immobilized on titanium by using a plasma immersion ion implantation process. The particles have a population-dependent capability in activating the integrin α5 orchestrated MAPK/ERK signal cascade of osteoblast differentiation in rat bone marrow stem cells (BMSCs), and promoting osteointegration of titanium. It was demonstrated that the titanium-supported Ag NPs played an important role in motivating integrin α5 through triggering the galvanic hydrogen evolution reactions, which was found in positive correlation with the distribution density of the immobilized Ag NPs. Since cellular uptake is a key factor determining the cytotoxic performance of Ag NPs, the extracellular effects of immobilized Ag NPs on promoting osteogenesis provided new insights into the safe application of nanomaterials, and into designing and developing renewed antibacterial devices with selective toxicity.
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences , Shanghai 200050, China.
authors.forename: Huiliang
authors.initials: H
authors.lastname: Cao
authors.affiliation: Department of Prosthodontics, School of Medicine, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University , 639 Zhizaoju Road, Shanghai 200011, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences , Shanghai 200050, China.
authors.forename: Fanhao
authors.initials: F
authors.lastname: Meng
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences , Shanghai 200050, China.
authors.forename: Jinshu
authors.initials: J
authors.lastname: Guo
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences , Shanghai 200050, China.
authors.forename: Donghui
authors.initials: D
authors.lastname: Wang
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences , Shanghai 200050, China.
authors.forename: Shi
authors.initials: S
authors.lastname: Qian
authors.affiliation: Department of Prosthodontics, School of Medicine, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University , 639 Zhizaoju Road, Shanghai 200011, China.
authors.forename: Xinquan
authors.initials: X
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences , Shanghai 200050, China.
authors.forename: Xuanyong
authors.identifier: http://orcid.org/0000-0001-9440-8143
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Physics and Materials Science, City University of Hong Kong , Tat Chee Avenue, Kowloon, Hong Kong, China.
authors.forename: Paul K
authors.initials: PK
authors.lastname: Chu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents; D002384:Catalysis; D053768:Metal Nanoparticles; D010012:Osteogenesis; D051381:Rats; D012834:Silver; D014025:Titanium
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver; D014025:Titanium
keywords: differentiation; integrin; osteointegration; silver; stem cells; titanium