title: Fluorescent aptasensor for 17β-estradiol determination based on gold nanoparticles quenching the fluorescence of Rhodamine B.
abstract: In this paper, we developed a fluorescent aptasensor for 17β-estradiol (E2) determination in aqueous solution using label-free E2-specific aptamer, gold nanoparticles (AuNPs) and Rhodamine B (RhoB) as sensing probe, fluorescent quencher and fluorescent indicator respectively. In the absence of E2, AuNPs were wrapped by E2 aptamer and maintained dispersed in NaCl solution basically. These dispersed AuNPs could effectively impair the originally high fluorescence of RhoB. Contrarily, in the presence of E2, E2 aptamer could specifically combine with E2 to form E2-aptamer complex, so the AuNPs were released by E2 aptamer and aggregated under the influence of NaCl. The aggregated AuNPs have a weak influence on RhoB fluorescence. Therefore, the E2 concentration can be determined by the change of fluorescence intensity of RhoB. This fluorescent assay has a detection limit as low as 0.48 nM, a linear range from 0.48 to 200 nM, and high selectivity over other disrupting chemicals. It was applied to determine E2 in water samples with recoveries in the range of 94.3-111.7%. The fluorescent aptasensor holds great potential for E2 detection in environmental water samples.
authors.affiliation: School of Agriculture and Biology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai, 200240, PR China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Ni
authors.affiliation: Shanghai Academy of Environmental Sciences, Shanghai, 200233, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Xia
authors.affiliation: School of Agriculture and Biology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai, 200240, PR China. Electronic address: zjuwlm@163.com.
authors.forename: Lumei
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai, 201418, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ye
authors.affiliation: School of Agriculture and Biology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai, 200240, PR China.
authors.forename: Gaoshang
authors.initials: G
authors.lastname: Du
authors.affiliation: School of Agriculture and Biology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai, 200240, PR China.
authors.forename: Haiwei
authors.initials: H
authors.lastname: Feng
authors.affiliation: School of Agriculture and Biology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai, 200240, PR China.
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Zhou
authors.affiliation: School of Agriculture and Biology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai, 200240, PR China.
authors.forename: Tong
authors.initials: T
authors.lastname: Zhang
authors.affiliation: School of Agriculture and Biology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai, 200240, PR China.
authors.forename: Wenhao
authors.initials: W
authors.lastname: Wang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D004958:Estradiol / Q000032:analysis*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D012235:Rhodamines; D004958:Estradiol; D006046:Gold; C029773:rhodamine B
keywords: Aptamer; Detection; Estrogen; Fluorescent dye; Sensing system