title: Photosynthetic CO2Conversion to Fatty Acid Ethyl Esters (FAEEs) Using Engineered Cyanobacteria.
abstract: Metabolic engineering of cyanobacteria has received attention as a sustainable strategy to convert carbon dioxide to fatty acid-derived chemicals that are widely used in the food and chemical industries. Herein, Synechococcus elongatus PCC 7942, a model cyanobacterium, was engineered for the first time to produce fatty acid ethyl esters (FAEEs) from CO2. Due to the lack of an endogenous ethanol production pathway and wax ester synthase (AftA) activity in the wild-type cyanobacterium, we metabolically engineered S. elongatus PCC 7942 by expressing heterologous AftA and introducing the ethanol pathway, resulting in detectable peaks of FAEEs. To enhance FAEE production, a heterologous phosphoketolase pathway was introduced in the FAEE-producing strain to supply acetyl-CoA. Subsequent optimization of the cyanobacterial culture with a hexadecane overlay resulted in engineered S. elongatus PCC 7942 that produced photosynthetic FAEEs (10.0 ± 0.7 mg/L/OD730) from CO2. This paper is the first report of photosynthetic production of FAEEs from CO2in cyanobacteria.
authors.affiliation: Clean Energy Research Center, Korea Institute of Science and Technology , Hwarang-ro 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea.
authors.forename: Hyun Jeong
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Clean Energy Research Center, Korea Institute of Science and Technology , Hwarang-ro 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea.
authors.forename: Jaeyeon
authors.initials: J
authors.lastname: Choi
authors.affiliation: Clean Energy Research Center, Korea Institute of Science and Technology , Hwarang-ro 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea.
authors.forename: Sun-Mi
authors.initials: SM
authors.lastname: Lee
authors.affiliation: Clean Energy Research Center, Korea Institute of Science and Technology , Hwarang-ro 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea.
authors.forename: Youngsoon
authors.initials: Y
authors.lastname: Um
authors.affiliation: Department of Chemical and Biological Engineering, Korea University , 145 Anam-ro, Seongbuk-gu, Seoul 02841, Republic of Korea.
authors.forename: Sang Jun
authors.initials: SJ
authors.lastname: Sim
authors.affiliation: Clean Energy Research Center, Korea Institute of Science and Technology , Hwarang-ro 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea.
authors.forename: Yunje
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University (SKKU) , 2066 Seobu-ro, Jangan-gu, Suwon 16419, Republic of Korea.
authors.forename: Han Min
authors.identifier: http://orcid.org/0000-0002-8797-0477
authors.initials: HM
authors.lastname: Woo
mesh_terms: D000105:Acetyl Coenzyme A / Q000378:metabolism; D000446:Aldehyde-Lyases / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism*; D004952:Esters / Q000737:chemistry / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods*; D010788:Photosynthesis; D046940:Synechococcus / Q000235:genetics / Q000378:metabolism*
chemical_list: D004952:Esters; D005227:Fatty Acids; D002245:Carbon Dioxide; D000431:Ethanol; D000105:Acetyl Coenzyme A; D000446:Aldehyde-Lyases; C022367:phosphoketolase
keywords: CO2 conversion; cyanobacteria; fatty acid ethyl ester; metabolic engineering