title: Purification and immunochemical characterization of Pla l 2, the profilin from Plantago lanceolata.
abstract: Profilins are small actin-binding proteins found in eukaryotes and involved in cell development, cytokinesis, membrane trafficking, and cell motility. From an allergenic point of view, profilins are panallergens usually involved in allergic polysensitization, although they are generally recognized as minor allergens. The objectives of this study were to identify and characterize the profilin from Plantago lanceolata pollen and to investigate the cross-reactivity between profilins from different pollen allergenic sources. Profilins from P. lancelolata (Pla l 2) and palm tree pollen (Pho d 2) were purified by affinity chromatography, deeply characterized and identified by mass spectrometry. Pla l 2 allergenicity was confirmed by immunoblot with serum samples from a patient population sensitized to profilin. Immunoblot inhibition was performed to study IgG reactivity between different pollen profilins. IgE cross-reactivity was demonstrated by ImmunoCAP inhibition. Pla l 2 is the second P. lanceolata allergen included in the IUIS Allergen Nomenclature database. Four peptides from purified Pla l 2 were identified with percentages of homology with other pollen profilins between 73 and 86%. Eighty-six percent (21/24) of the patient population recognized Pla l 2. The allergenic relatedness between Pla l 2, Pho d 2 and six pollen profilins was confirmed, and IgE cross-reactivity of Pla l 2 with rBet v 2 and rPhl p 12 was demonstrated. Pla l 2 is the profilin from P. lanceolata. The demonstrated allergenicity of this protein and its cross-reactivity with other pollen profilins support its use in profilin diagnostic assays.
authors.affiliation: R&D Department, Laboratorios LETI, S.L.U., C/del Sol 5, Tres Cantos, 28760, Madrid, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Moya
authors.affiliation: R&D Department, Laboratorios LETI, S.L.U., C/del Sol 5, Tres Cantos, 28760, Madrid, Spain.
authors.forename: Virginia
authors.initials: V
authors.lastname: Rubio
authors.affiliation: Allergy Service, Hospital de Guadalajara, Guadalajara, Spain.
authors.forename: Juan Mª
authors.initials: JM
authors.lastname: Beitia
authors.affiliation: R&D Department, Laboratorios LETI, S.L.U., C/del Sol 5, Tres Cantos, 28760, Madrid, Spain. Electronic address: jcarnes@leti.com.
authors.forename: Jerónimo
authors.initials: J
authors.lastname: Carnés
authors.affiliation: R&D Department, Laboratorios LETI, S.L.U., C/del Sol 5, Tres Cantos, 28760, Madrid, Spain.
authors.forename: M Angeles
authors.initials: MA
authors.lastname: López-Matas
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D052179:Antigens, Plant / Q000276:immunology / Q000302:isolation & purification; D003429:Cross Reactions; D005260:Female; D006023:Glycoproteins / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D015151:Immunoblotting; D008297:Male; D010940:Plant Proteins / Q000276:immunology*; D010943:Plantago / Q000276:immunology*; D011058:Pollen / Q000276:immunology; D051304:Profilins / Q000276:immunology* / Q000302:isolation & purification; D011817:Rabbits; D055815:Young Adult
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D006023:Glycoproteins; D010940:Plant Proteins; D051304:Profilins
keywords: Cross-reactivity; Molecular diagnosis; Panallergen; Plantago lanceolata; Profilin