title: Single α-particle irradiation permits real-time visualization of RNF8 accumulation at DNA damaged sites.
abstract: As well as being a significant source of environmental radiation exposure, α-particles are increasingly considered for use in targeted radiation therapy. A better understanding of α-particle induced damage at the DNA scale can be achieved by following their tracks in real-time in targeted living cells. Focused α-particle microbeams can facilitate this but, due to their low energy (up to a few MeV) and limited range, α-particles detection, delivery, and follow-up observations of radiation-induced damage remain difficult. In this study, we developed a thin Boron-doped Nano-Crystalline Diamond membrane that allows reliable single α-particles detection and single cell irradiation with negligible beam scattering. The radiation-induced responses of single 3 MeV α-particles delivered with focused microbeam are visualized in situ over thirty minutes after irradiation by the accumulation of the GFP-tagged RNF8 protein at DNA damaged sites.
authors.affiliation: Université de Bordeaux, Centre d'Etudes Nucléaires Bordeaux Gradignan (CENBG), Chemin du Solarium, 33175 Gradignan, France.|CNRS, UMR5797, Centre d'Etudes Nucléaires Bordeaux Gradignan (CENBG), Chemin du Solarium, 33175 Gradignan, France.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Muggiolu
authors.affiliation: CEA-LIST, Diamond Sensors Laboratory, Gif-sur-Yvette F-91191, France.
authors.forename: Michal
authors.initials: M
authors.lastname: Pomorski
authors.affiliation: Université de Bordeaux, Centre d'Etudes Nucléaires Bordeaux Gradignan (CENBG), Chemin du Solarium, 33175 Gradignan, France.|CNRS, UMR5797, Centre d'Etudes Nucléaires Bordeaux Gradignan (CENBG), Chemin du Solarium, 33175 Gradignan, France.
authors.forename: Gérard
authors.initials: G
authors.lastname: Claverie
authors.affiliation: CEA-LIST, Diamond Sensors Laboratory, Gif-sur-Yvette F-91191, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Berthet
authors.affiliation: CEA-LIST, Diamond Sensors Laboratory, Gif-sur-Yvette F-91191, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Mer-Calfati
authors.affiliation: CEA-LIST, Diamond Sensors Laboratory, Gif-sur-Yvette F-91191, France.
authors.forename: Samuel
authors.initials: S
authors.lastname: Saada
authors.affiliation: Université de Bordeaux, Centre d'Etudes Nucléaires Bordeaux Gradignan (CENBG), Chemin du Solarium, 33175 Gradignan, France.|CNRS, UMR5797, Centre d'Etudes Nucléaires Bordeaux Gradignan (CENBG), Chemin du Solarium, 33175 Gradignan, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Devès
authors.affiliation: Université de Bordeaux, Centre d'Etudes Nucléaires Bordeaux Gradignan (CENBG), Chemin du Solarium, 33175 Gradignan, France.|CNRS, UMR5797, Centre d'Etudes Nucléaires Bordeaux Gradignan (CENBG), Chemin du Solarium, 33175 Gradignan, France.
authors.forename: Marina
authors.initials: M
authors.lastname: Simon
authors.affiliation: Université de Bordeaux, Centre d'Etudes Nucléaires Bordeaux Gradignan (CENBG), Chemin du Solarium, 33175 Gradignan, France.|CNRS, UMR5797, Centre d'Etudes Nucléaires Bordeaux Gradignan (CENBG), Chemin du Solarium, 33175 Gradignan, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Seznec
authors.affiliation: Université de Bordeaux, Centre d'Etudes Nucléaires Bordeaux Gradignan (CENBG), Chemin du Solarium, 33175 Gradignan, France.|CNRS, UMR5797, Centre d'Etudes Nucléaires Bordeaux Gradignan (CENBG), Chemin du Solarium, 33175 Gradignan, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Barberet
mesh_terms: D000512:Alpha Particles / Q000009:adverse effects; D045744:Cell Line, Tumor; D004249:DNA Damage / Q000528:radiation effects*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D017930:Genes, Reporter; D006657:Histones / Q000378:metabolism; D006801:Humans; D008567:Membranes, Artificial; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D059008:Time-Lapse Imaging; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D006657:Histones; D008567:Membranes, Artificial; C486521:RNF8 protein, human; D011993:Recombinant Fusion Proteins; D044767:Ubiquitin-Protein Ligases