title: Characterization of fast-twitch and slow-twitch skeletal muscles of calsequestrin 2 (CASQ2)-knock out mice: unexpected adaptive changes of fast-twitch muscles only.
abstract: This study investigates the functional role of calsequestrin 2 (CASQ2) in both fast-twitch and slow-twitch skeletal muscles by using CASQ2-/- mice; CASQ2 is expressed throughout life in slow-twitch muscles, but only in the developmental and neonatal stages in fast-twitch muscles. CASQ2-/- causes increase in calsequestrin 1 (CASQ1) expression, but without functional changes in both muscle types. CASQ2-/- mice have ultrastructural changes in fast-twitch muscles only, i.e., formation of pentads and stacks in the sarcoplasmic reticulum.
authors.affiliation: Dipartimento di Scienze Biomediche dell'Università di Padova, Istituto Interuniversitario di Miologia, Viale G. Colombo 3, 35121, Padova, Italy.
authors.forename: Giorgia
authors.initials: G
authors.lastname: Valle
authors.affiliation: Consorzio MIA (Microscopy Image Analysis), Università di Milano-Bicocca, 20052, Monza, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Vergani
authors.affiliation: Dipartimento di Biomedicina Comparata ed Alimentazione dell'Università di Padova, Padova, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Sacchetto
authors.affiliation: Dipartimento di Scienze Biomediche dell'Università di Padova, Istituto Interuniversitario di Miologia, Viale G. Colombo 3, 35121, Padova, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Reggiani
authors.affiliation: Dipartimento di Scienze Biomediche dell'Università di Padova, Istituto Interuniversitario di Miologia, Viale G. Colombo 3, 35121, Padova, Italy.
authors.forename: Edith
authors.initials: E
authors.lastname: De Rosa
authors.affiliation: Dipartimento di Biomedicina Comparata ed Alimentazione dell'Università di Padova, Padova, Italy.
authors.forename: Lisa
authors.initials: L
authors.lastname: Maccatrozzo
authors.affiliation: Dipartimento di Scienze Biomediche dell'Università di Padova, Istituto Interuniversitario di Miologia, Viale G. Colombo 3, 35121, Padova, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Nori
authors.affiliation: Consorzio MIA (Microscopy Image Analysis), Università di Milano-Bicocca, 20052, Monza, Italy.
authors.forename: Antonello
authors.initials: A
authors.lastname: Villa
authors.affiliation: Dipartimento di Scienze Biomediche dell'Università di Padova, Istituto Interuniversitario di Miologia, Viale G. Colombo 3, 35121, Padova, Italy. pompeo.volpe@unipd.it.
authors.forename: Pompeo
authors.identifier: 0000-0003-0151-1585
authors.initials: P
authors.lastname: Volpe
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002155:Calsequestrin / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000502:physiology; D012519:Sarcoplasmic Reticulum / Q000378:metabolism
chemical_list: D002135:Calcium-Binding Proteins; D002155:Calsequestrin; D009124:Muscle Proteins; C514388:casq2 protein, mouse
keywords: Adaptation; Calsequestrin; Knock-out mice; Skeletal muscle