title: One-step post-imprint modification achieve dual-function of glycoprotein fluorescent sensor by "Click Chemistry".
abstract: A novel molecular imprinting fluorescent sensor synthesized avoiding complicated design and synthesis of a fluorescent functional monomer, and without the extra cost of additional fluorescence materials (quantum dots, carbon dots and fluorescein), only by a click reaction making the common protein imprinting polymers upgraded to a fluorescent sensing material which can be applied to the fast detection of glycoproteins. In this study, a common protein imprinted polymer (protein-IP) which containing a disulfide bond in the imprinted cavities was first synthesized, then the disulfide linkage was cleaved by reduction, the exposed thiol groups were used for the click reaction to introduce 4-vinyl phenylboronic acid to improve the imprinted recognition for glycoproteins and convert recognition behavior into fluorescent signal simultaneously in one-step site-specific post-imprinting modifications (PIMs). Good linear relationship was obtained in the range of 0.1-10mgmL-1by fluorescence assay, comparison of the adsorption capacity of the MIPs before and after modification proved that the method has no negative impact on the imprinting effect and exhibit good imprinting effect for glycoproteins of different molecular size and weight.
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China.
authors.forename: Junping
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China.
authors.forename: Jianli
authors.initials: J
authors.lastname: He
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China.
authors.forename: Qiliang
authors.initials: Q
authors.lastname: Deng
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China. Electronic address: s.wang@tust.edu.cn.
authors.forename: Shuo
authors.initials: S
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D057930:Click Chemistry / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D006023:Glycoproteins / Q000032:analysis*; D054802:Molecular Imprinting / Q000379:methods*; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry*; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence / Q000379:methods; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D006023:Glycoproteins; D011108:Polymers; D013438:Sulfhydryl Compounds
keywords: Click Chemistry; Glycoprotein; Molecularly imprinted polymer; Post-imprinting modifications