title: Anti-Melanogenic Effect ofOenothera laciniataMethanol Extract in Melan-a Cells.
abstract: We evaluated the antioxidant activity and anti-melanogenic effects ofOenothera laciniatamethanol extract (OLME)in vitroby using melan-a cells. The total polyphenol and flavonoid content of OLME was 66.3 and 19.0 mg/g, respectively. The electron-donating ability, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical-scavenging activity, and superoxide dismutase (SOD)-like activity of OLME (500 μg/mL) were 94.5%, 95.6%, and 63.6%, respectively. OLME and arbutin treatment at 50 μg/mL significantly decreased melanin content by 35.5% and 14.2%, respectively, compared to control (p< 0.05). OLME and arbutin treatment at 50 μg/mL significantly inhibited intra-cellular tyrosinase activity by 22.6% and 12.6%, respectively, compared to control (p< 0.05). OLME (50 μg/mL) significantly decreased tyrosinase, tyrosinase-related protein-1 (TRP-1), TRP-2, and microphthalmia-associated transcription factor-M (MITF-M) mRNA expression by 57.1%, 67.3%, 99.0%, and 77.0%, respectively, compared to control (p< 0.05). Arbutin (50 μg/mL) significantly decreased tyrosinase, TRP-1, and TRP-2 mRNA expression by 24.2%, 42.9%, and 48.5%, respectively, compared to control (p< 0.05). However, arbutin (50 μg/mL) did not affect MITF-M mRNA expression. Taken together, OLME showed a good antioxidant activity and anti-melanogenic effect in melan-a cells that was superior to that of arbutin, a well-known skin-whitening agent. The potential mechanism underlying the anti-melanogenic effect of OLME was inhibition of tyrosinase activity and down-regulation of tyrosinase, TRP-1, TRP-2, and MITF-M mRNA expression.
authors.affiliation: Department of Public Health, Graduate School, Keimyung University, Daegu, Korea.
authors.forename: Su Eun
authors.initials: SE
authors.lastname: Kim
authors.affiliation: Department of Beauty Coordination, Keimyung College University, Daegu, Korea.
authors.forename: Chae Myoung
authors.initials: CM
authors.lastname: Lee
authors.affiliation: Department of Public Health, Graduate School, Keimyung University, Daegu, Korea.
authors.forename: Young Chul
authors.initials: YC
authors.lastname: Kim
keywords: Anti-melanogenic effect; Antioxidant activity; Melan-a cells; Tyrosinase activity; Tyrosinase-related protein