title: Suppression of Nrf2 attenuates adipogenesis and decreases FGF21 expression through PPAR gamma in 3T3-L1 cells.
abstract: Adipogenesis is the process of differentiation from preadipocytes to adipocytes and is orchestrated by various transcription factors, such as the peroxisome proliferator-activated receptor gamma (PPARγ) and the CCAAT-enhancer-binding protein alpha (C/EBPα). Oxidative stress is also a crucial factor in adipogenesis, and adipocyte differentiation is affected by the cellular redox status. The nuclear factor E2-related factor 2 (Nrf2), which is a basic leucine zipper (bZIP) transcription factor, acts as a regulator of cellular oxidative stress. Although several previous studies examined the function of Nrf2 in adipogenesis, their results were controversial. In this study, we investigated whether the suppression of Nrf2 in 3T3-L1 cells affected adipogenesis. We found that adipogenesis master regulator genes, such as PPARγ and C/EBPα, were downregulated during the differentiation stage in Nrf2-knockdown 3T3-L1 cells. Moreover, the fibroblast growth factor 21 (FGF21) and manganese superoxide dismutase (MnSOD) were markedly downregulated in Nrf2-knockdown 3T3-L1 cells. Taken together, the results of the present study suggest that the suppression of Nrf2 attenuates adipogenesis and decreases FGF21 expression through PPARγ in 3T3-L1 cells.
authors.affiliation: Department of Internal Medicine, Seoul National University, College of Medicine, Seoul National University Bundang Hospital, Seongnam, South Korea.
authors.forename: Bo-Rahm
authors.initials: BR
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, Seoul National University, College of Medicine, Seoul National University Bundang Hospital, Seongnam, South Korea.
authors.forename: Gha Young
authors.initials: GY
authors.lastname: Lee
authors.affiliation: Department of Internal Medicine, Seoul National University, College of Medicine, Seoul, South Korea.
authors.forename: Hyeree
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Internal Medicine, Seoul National University, College of Medicine, Seoul National University Bundang Hospital, Seongnam, South Korea.
authors.forename: Hyo Jin
authors.initials: HJ
authors.lastname: Maeng
authors.affiliation: Department of Internal Medicine, Seoul National University, College of Medicine, Seoul National University Bundang Hospital, Seongnam, South Korea; Department of Internal Medicine, Seoul National University, College of Medicine, Seoul, South Korea.
authors.forename: Tae Jung
authors.initials: TJ
authors.lastname: Oh
authors.affiliation: Department of Internal Medicine, Seoul National University, College of Medicine, Seoul National University Bundang Hospital, Seongnam, South Korea; Department of Internal Medicine, Seoul National University, College of Medicine, Seoul, South Korea.
authors.forename: Kyoung Min
authors.initials: KM
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, Seoul National University, College of Medicine, Seoul National University Bundang Hospital, Seongnam, South Korea; Department of Internal Medicine, Seoul National University, College of Medicine, Seoul, South Korea.
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Moon
authors.affiliation: Department of Internal Medicine, Seoul National University, College of Medicine, Seoul National University Bundang Hospital, Seongnam, South Korea; Department of Internal Medicine, Seoul National University, College of Medicine, Seoul, South Korea.
authors.forename: Soo
authors.initials: S
authors.lastname: Lim
authors.affiliation: Department of Internal Medicine, Seoul National University, College of Medicine, Seoul National University Bundang Hospital, Seongnam, South Korea; Department of Internal Medicine, Seoul National University, College of Medicine, Seoul, South Korea.
authors.forename: Hak Chul
authors.initials: HC
authors.lastname: Jang
authors.affiliation: Department of Internal Medicine, Seoul National University, College of Medicine, Seoul National University Bundang Hospital, Seongnam, South Korea; Department of Internal Medicine, Seoul National University, College of Medicine, Seoul, South Korea. Electronic address: drshchoi@snu.ac.kr.
authors.forename: Sung Hee
authors.initials: SH
authors.lastname: Choi
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D015536:Down-Regulation / Q000187:drug effects; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D020868:Gene Silencing; D051379:Mice; D051267:NF-E2-Related Factor 2 / Q000235:genetics*; D018384:Oxidative Stress; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000187:drug effects
chemical_list: D051267:NF-E2-Related Factor 2; C495636:Nfe2l2 protein, mouse; D047495:PPAR gamma; C414620:fibroblast growth factor 21; D005346:Fibroblast Growth Factors; D013482:Superoxide Dismutase
keywords: Adipocyte differentiation; C/EBPα; FGF-21; NRF2; PPARγ