title: Zika Virus Tissue and Blood Compartmentalization in Acute Infection of Rhesus Macaques.
abstract: Animal models of Zika virus (ZIKV) are needed to better understand tropism and pathogenesis and to test candidate vaccines and therapies to curtail the pandemic. Humans and rhesus macaques possess similar fetal development and placental biology that is not shared between humans and rodents. We inoculated 2 non-pregnant rhesus macaques with a 2015 Brazilian ZIKV strain. Consistent with most human infections, the animals experienced no clinical disease but developed short-lived plasma viremias that cleared as neutralizing antibody developed. In 1 animal, viral RNA (vRNA) could be detected longer in whole blood than in plasma. Despite no major histopathologic changes, many adult tissues contained vRNA 14 days post-infection with highest levels in hemolymphatic tissues. These observations warrant further studies to investigate ZIKV persistence and its potential clinical implications for transmission via blood products or tissue and organ transplants.
authors.affiliation: Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, California, United States of America.
authors.forename: Lark L
authors.initials: LL
authors.lastname: Coffey
authors.affiliation: Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, California, United States of America.
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Pesavento
authors.affiliation: California National Primate Research Center, University of California, Davis, California, United States of America.
authors.forename: Rebekah I
authors.initials: RI
authors.lastname: Keesler
authors.affiliation: Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, California, United States of America.
authors.forename: Anil
authors.initials: A
authors.lastname: Singapuri
authors.affiliation: California National Primate Research Center, University of California, Davis, California, United States of America.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Watanabe
authors.affiliation: Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, California, United States of America.
authors.forename: Rie
authors.initials: R
authors.lastname: Watanabe
authors.affiliation: California National Primate Research Center, University of California, Davis, California, United States of America.
authors.forename: JoAnn
authors.initials: J
authors.lastname: Yee
authors.affiliation: California National Primate Research Center, University of California, Davis, California, United States of America.|Department of Psychology, University of California, Davis, California, United States of America.
authors.forename: Eliza
authors.initials: E
authors.lastname: Bliss-Moreau
authors.affiliation: California National Primate Research Center, University of California, Davis, California, United States of America.
authors.forename: Christina
authors.initials: C
authors.lastname: Cruzen
authors.affiliation: California National Primate Research Center, University of California, Davis, California, United States of America.
authors.forename: Kari L
authors.initials: KL
authors.lastname: Christe
authors.affiliation: California National Primate Research Center, University of California, Davis, California, United States of America.
authors.forename: J Rachel
authors.initials: JR
authors.lastname: Reader
authors.affiliation: California National Primate Research Center, University of California, Davis, California, United States of America.
authors.forename: Wilhelm
authors.initials: W
authors.lastname: von Morgenland
authors.affiliation: California National Primate Research Center, University of California, Davis, California, United States of America.
authors.forename: Anne M
authors.initials: AM
authors.lastname: Gibbons
authors.affiliation: California National Primate Research Center, University of California, Davis, California, United States of America.
authors.forename: A Mark
authors.initials: AM
authors.lastname: Allen
authors.affiliation: Hologic, San Diego, California, United States of America.
authors.forename: Jeff
authors.initials: J
authors.lastname: Linnen
authors.affiliation: Hologic, San Diego, California, United States of America.
authors.forename: Kui
authors.initials: K
authors.lastname: Gao
authors.affiliation: Blood Systems Research Institute and University of California, San Francisco, California, United States of America.
authors.forename: Eric
authors.initials: E
authors.lastname: Delwart
authors.affiliation: Blood Systems Research Institute and University of California, San Francisco, California, United States of America.
authors.forename: Graham
authors.initials: G
authors.lastname: Simmons
authors.affiliation: Blood Systems Research Institute and University of California, San Francisco, California, United States of America.
authors.forename: Mars
authors.initials: M
authors.lastname: Stone
authors.affiliation: Blood Systems Research Institute and University of California, San Francisco, California, United States of America.
authors.forename: Marion
authors.initials: M
authors.lastname: Lanteri
authors.affiliation: Blood Systems Research Institute and University of California, San Francisco, California, United States of America.
authors.forename: Sonia
authors.initials: S
authors.lastname: Bakkour
authors.affiliation: Blood Systems Research Institute and University of California, San Francisco, California, United States of America.
authors.forename: Michael
authors.initials: M
authors.lastname: Busch
authors.affiliation: California National Primate Research Center, University of California, Davis, California, United States of America.
authors.forename: John
authors.initials: J
authors.lastname: Morrison
authors.affiliation: California National Primate Research Center, University of California, Davis, California, United States of America.
authors.forename: Koen K A
authors.identifier: http://orcid.org/0000-0002-7375-1337
authors.initials: KK
authors.lastname: Van Rompay
mesh_terms: D000208:Acute Disease; D000375:Aging / Q000473:pathology; D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D005260:Female; D008253:Macaca mulatta; D009928:Organ Specificity; D012367:RNA, Viral / Q000097:blood / Q000652:urine; D012463:Saliva / Q000821:virology; D014018:Tissue Distribution; D014766:Viremia / Q000097:blood; D000071244:Zika Virus / Q000276:immunology / Q000502:physiology*; D000071243:Zika Virus Infection / Q000097:blood* / Q000821:virology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D012367:RNA, Viral