title: Trichoderma harzianum containing 1-aminocyclopropane-1-carboxylate deaminase and chitinase improved growth and diminished adverse effect caused by Fusarium oxysporum in soybean.
abstract: An isolate, named Trichoderma harzianum T-soybean, showed growth-promoting for soybean seedlings and induced resistance to Fusarium oxysporum under greenhouse. Compared to control soybean seedlings, fresh weight, dry weight, lateral root number, chlorophyll content, root activity and soluble protein of plants pretreated with T-soybean increased, but initial pod height reduced. Furthermore, we found that T-soybean inhibited the growth of F. oxysporum by parasitic function. In addition, plate test results showed that culture filtrates of T-soybean also inhibited significantly F. oxysporum growth. Meanwhile, T-soybean treatment obviously reduced disease severity and induced quickly the H2O2and O2-burst as well as pathogenesis related protein gene (PR3) expression after F. oxysporum inoculation, and subsequently diminished the cell damage in soybean caused by the pathogen challenge. Reactive oxygen species (ROS) scavenging enzymes activity analysis showed that the activities of peroxidase (POD), polyphenol oxidase (PPO) and superoxide dismutase (SOD) increased significantly in T-soybean pretreated plants. These results suggested that T-soybean treatment induced resistance in soybean seedlings to F. oxysporum by companying the production of ROS and the increasing of ROS scavenging enzymes activity as well as PR3 expression.
authors.affiliation: College of Life Science and Agronomy, Zhoukou Normal University, Wenchangjie Dongduan, ZhouKou 466001, China. Electronic address: zhangfl2007@126.com.
authors.forename: Fuli
authors.initials: F
authors.lastname: Zhang
authors.affiliation: College of Life Science and Agronomy, Zhoukou Normal University, Wenchangjie Dongduan, ZhouKou 466001, China.
authors.forename: Can
authors.initials: C
authors.lastname: Chen
authors.affiliation: College of Life Science and Agronomy, Zhoukou Normal University, Wenchangjie Dongduan, ZhouKou 466001, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Henan Lotus Gourmet Powder Inc., XiangCheng 466200, China.
authors.forename: Lidong
authors.initials: L
authors.lastname: Gao
authors.affiliation: Henan Lotus Gourmet Powder Inc., XiangCheng 466200, China.
authors.forename: Jidong
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Life Science and Agronomy, Zhoukou Normal University, Wenchangjie Dongduan, ZhouKou 466001, China. Electronic address: chenlongzg@126.com.
authors.forename: Long
authors.initials: L
authors.lastname: Chen
authors.affiliation: College of Life Science and Agronomy, Zhoukou Normal University, Wenchangjie Dongduan, ZhouKou 466001, China.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Fan
authors.affiliation: College of Life Science and Agronomy, Zhoukou Normal University, Wenchangjie Dongduan, ZhouKou 466001, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: College of Life Science and Agronomy, Zhoukou Normal University, Wenchangjie Dongduan, ZhouKou 466001, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zhang
authors.affiliation: College of Life Science and Agronomy, Zhoukou Normal University, Wenchangjie Dongduan, ZhouKou 466001, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: He
authors.affiliation: College of Life Science and Agronomy, Zhoukou Normal University, Wenchangjie Dongduan, ZhouKou 466001, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: College of Life Science and Agronomy, Zhoukou Normal University, Wenchangjie Dongduan, ZhouKou 466001, China.
authors.forename: Xiue
authors.initials: X
authors.lastname: Ji
mesh_terms: D019755:Carbon-Carbon Lyases / Q000378:metabolism; D002688:Chitinases / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism*; D005670:Fusarium / Q000502:physiology*; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control*; D013025:Glycine max / Q000254:growth & development* / Q000382:microbiology*; D014242:Trichoderma / Q000201:enzymology*
chemical_list: D005656:Fungal Proteins; D002688:Chitinases; C033634:1-aminocyclopropane-1-carboxylate deaminase; D019755:Carbon-Carbon Lyases
keywords: Biocontrol; Defense-related gene; Plant growth promotion; Reactive oxygen species; Soybean root rot; Trichoderma harzianum T-soybean