title: Gelsolin Inhibits the Inflammatory Process Induced by LPS.
abstract: OBJECTIVE
  Endotoxemia is a life-threatening situation that signifies a key challenge in the field of intensive care medicine. Proinflammatory mediators produced by macrophages play a key role in endotoxemia. Gelsolin (GSN) is involved in the process of inflammation.
  
  
  METHODS
  IL-6 and TNF-α in the supernatant were measured with an ELISA kit. NO production was assessed by measurement of nitrite concentration with the Griess assay. si-RNA directed against GSN (si-GSN) was transfected by Lipofectamine.
  
  
  RESULTS
  LPS decreased the levels of GSN. Recombinant GSN inhibited the cytokines induced by LPS and rescued mice from LPS-induced death, and si-GSN increased death in the LPS-pretreated mice.
  
  
  CONCLUSIONS
  GSN exhibited a protective role in endotoxemia.
authors.forename: Ying
authors.initials: Y
authors.lastname: Cheng
authors.forename: Xuguang
authors.initials: X
authors.lastname: Hu
authors.forename: Cong
authors.initials: C
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.forename: Jianing
authors.initials: J
authors.lastname: Wang
authors.forename: Mingyu
authors.initials: M
authors.lastname: Wang
authors.forename: Fu
authors.initials: F
authors.lastname: Gao
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Han
authors.forename: Chaoxiong
authors.initials: C
authors.lastname: Zhang
authors.forename: Ding
authors.initials: D
authors.lastname: Sun
authors.forename: Rui
authors.initials: R
authors.lastname: Min
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D019446:Endotoxemia / Q000209:etiology* / Q000401:mortality / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D018260:Gelsolin / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D015850:Interleukin-6 / Q000032:analysis; D008070:Lipopolysaccharides / Q000633:toxicity*; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D000067996:RAW 264.7 Cells; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D018260:Gelsolin; D015850:Interleukin-6; D008070:Lipopolysaccharides; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha
keywords: Gelsolin; IL-6; LPS; TNF-α