title: Decreased expression of VLDLR is inversely correlated with miR-200c in human colorectal cancer.
abstract: Colorectal cancer (CRC) is one of the most common cancers and has a high rate of morbidity and mortality worldwide. Very-low-density-lipoprotein receptor (VLDLR), a member of the low-density-lipoprotein receptor (LDLR) superfamily, is a multifunctional receptor that regulates cellular signaling by binding numerous ligands. Several studies reported the altered expression of VLDLR and suggested that VLDLR may play a critical role in tumor development by affecting cell proliferation and metastasis. However, the function of VLDLR and regulation of its expression by miRNAs have not been investigated in CRC. In the present study, we investigated the expression of VLDLR in CRC patients and found it to be significantly decreased in tumors in comparison with paired adjacent non-tumor tissues. Moreover, VLDLR over-expression inhibited the proliferation and migration of CRC cells. We also found that VLDLR expression was negatively regulated by miR-200c in CRC cells and that their expression levels were inversely correlated in CRC patients. These data suggest that VLDLR down-regulation mediated by the increased expression of miR-200c may be involved in the development of CRC.
authors.affiliation: Department of Medical Life Sciences, The Catholic University of Korea, Seoul, Korea.
authors.forename: Bong-Kyu
authors.initials: BK
authors.lastname: Kim
authors.affiliation: Department of Medical Life Sciences, The Catholic University of Korea, Seoul, Korea.
authors.forename: Hye-In
authors.initials: HI
authors.lastname: Yoo
authors.affiliation: Department of Medical Life Sciences, The Catholic University of Korea, Seoul, Korea.
authors.forename: Ah-Reum
authors.initials: AR
authors.lastname: Lee
authors.affiliation: Department of Medical Life Sciences, The Catholic University of Korea, Seoul, Korea.
authors.forename: Keonwoo
authors.initials: K
authors.lastname: Choi
authors.affiliation: Department of Medical Life Sciences, The Catholic University of Korea, Seoul, Korea.
authors.forename: Sungjoo Kim
authors.initials: SK
authors.lastname: Yoon
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D003106:Colon / Q000378:metabolism / Q000473:pathology; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D011973:Receptors, LDL / Q000235:genetics*; D012007:Rectum / Q000378:metabolism / Q000473:pathology
chemical_list: C533896:MIRN200 microRNA, human; D035683:MicroRNAs; D011973:Receptors, LDL; C036053:VLDL receptor
keywords: VLDLR; colorectal cancer; miR-200c; miRNA