title: A Label-Free Electrochemical Biosensor Based on a Reduced Graphene Oxide and Indole-5-Carboxylic Acid Nanocomposite for the Detection ofKlebsiella pneumoniae.
abstract: A label-free DNA hybridization electrochemical sensor for the detection of Klebsiella pneumoniae was developed, which could be helpful in the diagnosis of bacterial infections. Indole-5-carboxylic acid (ICA) and graphene oxide (GO) were electrodeposited on a glassy carbon electrode, and the resulting reduced GO (rGO)-ICA hybrid film served as a platform for immobilizing oligonucleotides on a single-stranded DNA (ssDNA) sequence. The conditions were optimized, with excellent electrochemical performance. A significant change was observed after hybridization of ssDNA with the target probe under optimum conditions. Hybridization with complementary, noncomplementary, one-base mismatched, and three-base mismatched DNA targets was studied effectively by differential pulse voltammetry. The proposed strategy could detect target DNA down to 3 × 10-11 M, with a linear range from 1 × 10-6 M to 1 × 10-10 M, showing high sensitivity. This electrochemical method is simple, free from indicator, and shows good selectivity. Hence, electrochemical biosensors are successfully demonstrated for the detection of K. pneumoniae.
authors.affiliation: Fudan University Pudong Medical Center, Shanghai Pudong Hospital, Department of Clinical Laboratory, 2800 Gongwei Rd, Huinan Town, Pudong, Shanghai 201399, China.
authors.forename: Ze
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: The First Affiliated Hospital of the Harbin Medical University, No. 23 Youzheng St, Harbin, Heilongjiang Province 150001, China.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Yu
authors.affiliation: The First Affiliated Hospital of the Harbin Medical University, No. 23 Youzheng St, Harbin, Heilongjiang Province 150001, China.
authors.forename: Guang-Cai
authors.initials: GC
authors.lastname: Wan
authors.affiliation: The First Affiliated Hospital of the Harbin Medical University, No. 23 Youzheng St, Harbin, Heilongjiang Province 150001, China.
authors.forename: Jing-Hui
authors.initials: JH
authors.lastname: Jiang
authors.affiliation: The First Affiliated Hospital of the Harbin Medical University, No. 23 Youzheng St, Harbin, Heilongjiang Province 150001, China.
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.affiliation: The First Hospital of Suihua City, Intersection of Renhe St and Changjiang North Rd, Suihua, Heilongjiang Province 152000, China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Liu
authors.affiliation: Fudan University Pudong Medical Center, Shanghai Pudong Hospital, Department of Clinical Laboratory, 2800 Gongwei Rd, Huinan Town, Pudong, Shanghai 201399, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Chang
authors.affiliation: Harbin Medical University, Public Health School, No. 157 Baojian Rd, Nangang District, Harbin, Heilongjiang Province 150081, China.
authors.forename: Hong-Zhi
authors.initials: HZ
authors.lastname: Pan
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D015342:DNA Probes / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000302:isolation & purification*; D053761:Nanocomposites / Q000737:chemistry*; D009693:Nucleic Acid Hybridization
chemical_list: D015342:DNA Probes; D004277:DNA, Single-Stranded; D007211:Indoles; C000619816:indole-5-carboxylic acid; D006108:Graphite