title: Monitoring HPV-16 E7 phosphorylation events.
abstract: HPV-16 E7 is one of the key proteins that, by interfering with the host metabolism through many protein-protein interactions, hijacks cell regulation and contributes to malignancy. Here we report the high resolution investigation of the CR3 region of HPV-16 E7, both as an isolated domain and in the full-length protein. This opens the way to the atomic level study of the many interactions in which HPV-16 E7 is involved. Along these lines we show here the effect of one of the key post-translational modifications of HPV-16 E7, the phosphorylation by casein kinase II.
authors.affiliation: Magnetic Resonance Center (CERM) and Department of Chemistry "Ugo Schiff", University of Florence, via Luigi Sacconi 6, Sesto Fiorentino, Italy.
authors.forename: Marcela O
authors.initials: MO
authors.lastname: Nogueira
authors.affiliation: Magnetic Resonance Center (CERM) and Department of Chemistry "Ugo Schiff", University of Florence, via Luigi Sacconi 6, Sesto Fiorentino, Italy.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Hošek
authors.affiliation: Magnetic Resonance Center (CERM) and Department of Chemistry "Ugo Schiff", University of Florence, via Luigi Sacconi 6, Sesto Fiorentino, Italy.
authors.forename: Eduardo O
authors.initials: EO
authors.lastname: Calçada
authors.affiliation: Magnetic Resonance Center (CERM) and Department of Chemistry "Ugo Schiff", University of Florence, via Luigi Sacconi 6, Sesto Fiorentino, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Castiglia
authors.affiliation: International Centre for Genetic Engineering and Biotechnology (ICGEB), Padriciano 99, Trieste, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Massimi
authors.affiliation: International Centre for Genetic Engineering and Biotechnology (ICGEB), Padriciano 99, Trieste, Italy.
authors.forename: Lawrence
authors.initials: L
authors.lastname: Banks
authors.affiliation: Magnetic Resonance Center (CERM) and Department of Chemistry "Ugo Schiff", University of Florence, via Luigi Sacconi 6, Sesto Fiorentino, Italy. Electronic address: felli@cerm.unifi.it.
authors.forename: Isabella C
authors.initials: IC
authors.lastname: Felli
authors.affiliation: Magnetic Resonance Center (CERM) and Department of Chemistry "Ugo Schiff", University of Florence, via Luigi Sacconi 6, Sesto Fiorentino, Italy. Electronic address: pierattelli@cerm.unifi.it.
authors.forename: Roberta
authors.initials: R
authors.lastname: Pierattelli
mesh_terms: D000595:Amino Acid Sequence; D047390:Casein Kinase II / Q000378:metabolism*; D002460:Cell Line; D052162:Human papillomavirus 16 / Q000378:metabolism*; D006801:Humans; D009369:Neoplasms / Q000473:pathology*; D019906:Nuclear Magnetic Resonance, Biomolecular; D050725:Papillomavirus E7 Proteins / Q000378:metabolism*; D010766:Phosphorylation; D054730:Protein Interaction Domains and Motifs; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary
chemical_list: D050725:Papillomavirus E7 Proteins; C059731:oncogene protein E7, Human papillomavirus type 16; D047390:Casein Kinase II
keywords: E7; HPV; IDP; Intrinsically Disordered Proteins; NMR; Nuclear Magnetic Resonance Spectroscopy; PTM; Phosphorylation