title: Differential Proteome Between Patient-Related and Non-related Environmental Isolates of Legionella pneumophila.
abstract: Molecular epidemiologic studies of Legionella have shown different molecular types coexisting in the same environment, with only one having the ability to trigger an outbreak. We therefore studied the proteome of isolates of these different molecular types in search of the proteins responsible for infection. In this study, we performed a differential proteomic analysis between patient-related and non-patient-related environmental isolates using two-dimensional difference gel electrophoresis (2D-DIGE) combined with mass spectrometry. Sixty-three spots were observed as being different between the two groups; 31 spots were identified corresponding to 23 different proteins. Patient-related isolates overexpressed proteins associated with metabolism, with enzymes of the tricarboxylic acid cycle and the degradation pathways being the most abundant proteins identified. However, the largest group of non-patient-related proteins was associated with stress response. Furthermore, the MOMP protein was located in different spots depending on their patient-related or non-patient-related origin, suggesting different post-translational modifications. According to these results, different bacterial adaptation pathways are activated in stress conditions which influence their ability to produce infection.
authors.affiliation: Unitat de Malalties Infeccioses, Fundació Institut Investigació en Ciències de la Salut Germans Trias i Pujol, Ctra Can Ruti. Camí escoles s/n, 08916, Badalona, Barcelona, Spain.|Universitat Autònoma de Barcelona, 08193, Cerdanyola, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Quero
authors.affiliation: Unitat de Malalties Infeccioses, Fundació Institut Investigació en Ciències de la Salut Germans Trias i Pujol, Ctra Can Ruti. Camí escoles s/n, 08916, Badalona, Barcelona, Spain. mgarcia@igtp.cat.|CIBER de Enfermedades Respiratorias, 28029, Madrid, Spain. mgarcia@igtp.cat.
authors.forename: Marian
authors.initials: M
authors.lastname: García-Núñez
authors.affiliation: Unitat de Malalties Infeccioses, Fundació Institut Investigació en Ciències de la Salut Germans Trias i Pujol, Ctra Can Ruti. Camí escoles s/n, 08916, Badalona, Barcelona, Spain.|CIBER de Enfermedades Respiratorias, 28029, Madrid, Spain.
authors.forename: Noemí
authors.initials: N
authors.lastname: Párraga-Niño
authors.affiliation: Universitat Autònoma de Barcelona, 08193, Cerdanyola, Spain.|Unitat de Malalties Infeccioses, Hospital Universitari Germans Trias i Pujol, 08916, Badalona, Spain.|CIBER de Enfermedades Respiratorias, 28029, Madrid, Spain.
authors.forename: M Luisa
authors.initials: ML
authors.lastname: Pedro-Botet
authors.affiliation: Universitat Autònoma de Barcelona, 08193, Cerdanyola, Spain.|Unitat de Malalties Infeccioses, Hospital Universitari Germans Trias i Pujol, 08916, Badalona, Spain.|CIBER de Enfermedades Respiratorias, 28029, Madrid, Spain.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Mateu
authors.affiliation: Universitat Autònoma de Barcelona, 08193, Cerdanyola, Spain.|Unitat de Malalties Infeccioses, Hospital Universitari Germans Trias i Pujol, 08916, Badalona, Spain.|CIBER de Enfermedades Respiratorias, 28029, Madrid, Spain.
authors.forename: Miquel
authors.initials: M
authors.lastname: Sabrià
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D004783:Environmental Microbiology; D006801:Humans; D016952:Legionella pneumophila / Q000302:isolation & purification* / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics
chemical_list: D020543:Proteome