title: Triglyceride dependent differentiation of obesity in adipose tissues by FTIR spectroscopy coupled with chemometrics.
abstract: The excess deposition of triglycerides in adipose tissue is the main reason of obesity and causes excess release of fatty acids to the circulatory system resulting in obesity and insulin resistance. Body mass index and waist circumference are not precise measure of obesity and obesity related metabolic diseases. Therefore, in the current study, it was aimed to propose triglyceride bands located at 1770-1720 cm-1spectral region as a more sensitive obesity related biomarker using the diagnostic potential of Fourier Transform Infrared (FTIR) spectroscopy in subcutaneous (SCAT) and visceral (VAT) adipose tissues. The adipose tissue samples were obtained from 10 weeks old male control (DBA/2J) (n = 6) and four different obese BFMI mice lines (n = 6 per group). FTIR spectroscopy coupled with hierarchical cluster analysis (HCA) and principal component analysis (PCA) was applied to the spectra of triglyceride bands as a diagnostic tool in the discrimination of the samples. Successful discrimination of the obese, obesity related insulin resistant and control groups were achieved with high sensitivity and specificity. The results revealed the power of FTIR spectroscopy coupled with chemometric approaches in internal diagnosis of abdominal obesity based on the spectral differences in the triglyceride region that can be used as a spectral marker.
authors.affiliation: Department of Biological Sciences, Middle East Technical University, 06531, Ankara, Turkey.
authors.forename: Fatma
authors.initials: F
authors.lastname: Kucuk Baloglu
authors.affiliation: Department of Biological Sciences, Middle East Technical University, 06531, Ankara, Turkey.
authors.forename: Onur
authors.initials: O
authors.lastname: Baloglu
authors.affiliation: Department of Breeding Biology and Molecular Genetics, Humboldt Universitatzu Berlin, Berlin, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Heise
authors.affiliation: Department of Breeding Biology and Molecular Genetics, Humboldt Universitatzu Berlin, Berlin, Germany.
authors.forename: Gudrun
authors.initials: G
authors.lastname: Brockmann
authors.affiliation: Department of Biological Sciences, Middle East Technical University, 06531, Ankara, Turkey.
authors.forename: Feride
authors.initials: F
authors.lastname: Severcan
mesh_terms: D000273:Adipose Tissue / Q000473:pathology*; D000818:Animals; D002454:Cell Differentiation; D016000:Cluster Analysis; D048088:Informatics; D051379:Mice; D009765:Obesity / Q000473:pathology*; D025341:Principal Component Analysis; D017550:Spectroscopy, Fourier Transform Infrared; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D014280:Triglycerides
keywords: Adipose Tissue; Diagnosis; FTIR Spectroscopy; Hierarchical Cluster Analysis ; Obesity; Principal Component Analysis