title: Adenylate Cyclase Type III Is Not a Ubiquitous Marker for All Primary Cilia during Development.
abstract: Adenylate cyclase type III (AC3) is localized in plasma membrane of neuronal primary cilium and can be used as a marker of this cilium. AC3 has also been detected in some other primary cilia such as those of fibroblasts, synoviocytes or astrocytes. Despite the presence of a cilium in almost all cell types, we show that AC3 is not a common marker of all primary cilia of different human and mouse tissues during development. In peripheral organs, AC3 is present mainly in primary cilia in cells of the mesenchymal lineage (fibroblasts, chondroblasts, osteoblasts-osteocytes, odontoblasts, muscle cells and endothelial cells). In epithelia, the apical cilium of renal and pancreatic tubules and of ductal plate in liver is AC3-negative whereas the cilium of basal cells of stratified epithelia is AC3-positive. Using fibroblasts cell culture, we show that AC3 appears at the plasma membrane of the primary cilium as soon as this organelle develops. The functional significance of AC3 localization at the cilium membrane in some cells but not others has to be investigated in relationship with cell physiology and expression at the cilium plasma membrane of specific upstream receptors.
authors.affiliation: Institut d'Histologie, Service Central de Microscopie Electronique, Faculté de Médecine, Université de Strasbourg, Strasbourg, France.|Fédération de Médecine Translationnelle de Strasbourg (FMTS), Strasbourg, France.|Hôpitaux Universitaires de Strasbourg, Strasbourg, France.|ICube laboratory UMR 7357, team IMIS, Strasbourg, France.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Antal
authors.affiliation: Institut d'Histologie, Service Central de Microscopie Electronique, Faculté de Médecine, Université de Strasbourg, Strasbourg, France.|Fédération de Médecine Translationnelle de Strasbourg (FMTS), Strasbourg, France.|Hôpitaux Universitaires de Strasbourg, Strasbourg, France.|ICube laboratory UMR 7357, team IMIS, Strasbourg, France.
authors.forename: Karelle
authors.initials: K
authors.lastname: Bénardais
authors.affiliation: Institut d'Histologie, Service Central de Microscopie Electronique, Faculté de Médecine, Université de Strasbourg, Strasbourg, France.|Fédération de Médecine Translationnelle de Strasbourg (FMTS), Strasbourg, France.|Hôpitaux Universitaires de Strasbourg, Strasbourg, France.|ICube laboratory UMR 7357, team IMIS, Strasbourg, France.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Samama
authors.affiliation: UMR CNRS 7213, Laboratoire de Biophotonique et Pharmacologie, Faculté de Pharmacie, Université de Strasbourg, Illkirch, France.
authors.forename: Cyril
authors.initials: C
authors.lastname: Auger
authors.affiliation: UMR CNRS 7213, Laboratoire de Biophotonique et Pharmacologie, Faculté de Pharmacie, Université de Strasbourg, Illkirch, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Schini-Kerth
authors.affiliation: ICube laboratory UMR 7357, team IMIS, Strasbourg, France.|Anatomy and Neurobiology, VCU, Richmond, VA, United States of America.
authors.forename: Said
authors.initials: S
authors.lastname: Ghandour
authors.affiliation: Institut d'Histologie, Service Central de Microscopie Electronique, Faculté de Médecine, Université de Strasbourg, Strasbourg, France.|Fédération de Médecine Translationnelle de Strasbourg (FMTS), Strasbourg, France.|Hôpitaux Universitaires de Strasbourg, Strasbourg, France.
authors.forename: Nelly
authors.initials: N
authors.lastname: Boehm
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D019070:Cell Lineage / Q000502:physiology*; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D002923:Cilia / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D059630:Mesenchymal Stem Cells; D051379:Mice; D010179:Pancreas / Q000166:cytology / Q000378:metabolism
chemical_list: D015415:Biomarkers; D000262:Adenylyl Cyclases; C421784:adenylate cyclase 3