title: Effects of Leukoreduction and Storage on Erythrocyte Phosphatidylserine Expression and Eicosanoid Concentrations in Units of Canine Packed Red Blood Cells.
abstract: BACKGROUND
  Storage of canine packed red blood cells (pRBCs) can increase erythrocyte phosphatidylserine (PS) expression and eicosanoid concentrations.
  
  
  OBJECTIVE
  To determine the effects of leukoreduction on erythrocyte PS expression and eicosanoid concentrations in stored units of canine pRBCs. Our hypothesis was that leukoreduction would decrease PS expression and eicosanoid concentrations.
  
  
  METHODS
  Eight healthy dogs.
  
  
  METHODS
  In a cross-over study, units of whole blood were leukoreduced (LR) or non-LR and stored (10 and 21 days) as pRBCs. Samples were collected at donation, and before and after a simulated transfusion. PS expression was measured by flow cytometry, and concentrations of arachidonic acid (AA), prostaglandin F2α(PGF2α), prostaglandin E2(PGE2), prostaglandin D2(PGD2), thromboxane B2(TXB2), 6-keto-prostaglandin F1α(6-keto-PGF1α), and leukotriene B4(LTB4) were quantified by liquid chromatography-mass spectrometry.
  
  
  RESULTS
  There was no change in PS expression during leukoreduction, storage, and simulated transfusion for non-LR and LR units. Immediately after leukoreduction, there was a significant increase in TXB2and PGF2αconcentrations, but during storage, these eicosanoids decreased to non-LR concentrations. In both LR and non-LR units, 6-keto-PGF1αconcentrations increased during storage and simulated transfusion, but there was no difference between unit type. There was no difference in AA, LTB4, PGE2, and PGD2concentrations between unit types.
  
  
  CONCLUSIONS
  Leukoreduction, storage, and simulated transfusion do not alter erythrocyte PS expression. Leukoreduction causes an immediate increase in concentrations of TXB2and PGF2α, but concentrations decrease to non-LR concentrations with storage. Leukoreduction does not decrease the accumulation of 6-keto-PGF1αduring storage.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS.
authors.forename: S M
authors.initials: SM
authors.lastname: Muro
authors.affiliation: Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS.
authors.forename: J H
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS.
authors.forename: J V
authors.initials: JV
authors.lastname: Stokes
authors.affiliation: Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS.
authors.forename: M K
authors.initials: MK
authors.lastname: Ross
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS.
authors.forename: T M
authors.initials: TM
authors.lastname: Archer
authors.affiliation: Department of Pathobiology and Population Medicine (Wills), College of Veterinary Medicine, Mississippi State University, Mississippi State, MS.
authors.forename: R W
authors.initials: RW
authors.lastname: Wills
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS.
authors.forename: A J
authors.initials: AJ
authors.lastname: Mackin
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS.
authors.forename: J M
authors.initials: JM
authors.lastname: Thomason
mesh_terms: D000818:Animals; D001793:Blood Preservation / Q000662:veterinary*; D018592:Cross-Over Studies; D004285:Dogs; D015777:Eicosanoids / Q000097:blood*; D017707:Erythrocyte Transfusion / Q000662:veterinary; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D047589:Leukocyte Reduction Procedures / Q000662:veterinary*; D008297:Male; D010718:Phosphatidylserines / Q000097:blood*
chemical_list: D015777:Eicosanoids; D010718:Phosphatidylserines
keywords: Dog; Prostacyclin; Thromboxane; Transfusion