title: Temperature of a Dengue Rapid Diagnostic Test under Tropical Climatic Conditions: A Follow Up Study.
abstract: The Dengue Duo Rapid Diagnostic Test (SD Dengue RDT) has good specificity and sensitivity for dengue diagnosis in rural tropical areas. In a previous study, using four control sera, we demonstrated that that the diagnostic accuracy of these RDTs remains stable after long-term storage at high temperatures. We extended this study by testing sera from 119 febrile patients collected between July-November 2012 at Salavan Provincial Hospital (southern Laos) with RDTs stored for 6 months at 4°C, 35° and in a hut (miniature traditional house) at Lao ambient temperatures. The dengue NS1 antigen results from RDTs stored at 35°C and in the hut demonstrated 100% agreement with those stored at 4°C. However, lower positive percent agreements, with broad 95%CI, were observed for the tests: IgM, 60% (14.7-94.7) and 40% (5.3-85.3) for RDTs store at 35°C and in the hut, compared to those stored at 4°C, respectively. This study strenghtens the evidence of the robustness of the NS1 antigen detection RDT for the diagnosis of dengue after storage at tropical temperatures.
authors.affiliation: Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR.
authors.forename: Onanong
authors.initials: O
authors.lastname: Sengvilaipaseuth
authors.affiliation: Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR.
authors.forename: Koukeo
authors.initials: K
authors.lastname: Phommasone
authors.affiliation: UMR "Emergence des Pathologies Virales" (EPV: Aix-Marseille university - IRD 190 - Inserm 1207 - EHESP), Marseille, France.|Institut hospitalo-universitaire Méditerranée infection, APHM Public Hospitals of Marseille, Marseille, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: de Lamballerie
authors.affiliation: Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR.
authors.forename: Manivanh
authors.initials: M
authors.lastname: Vongsouvath
authors.affiliation: Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR.
authors.forename: Ooyanong
authors.initials: O
authors.lastname: Phonemixay
authors.affiliation: Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR.|Centre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom.|Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Stuart D
authors.initials: SD
authors.lastname: Blacksell
authors.affiliation: Faculty of Postgraduate Studies, University of Health Sciences, Vientiane, Lao PDR.
authors.forename: Mayfong
authors.initials: M
authors.lastname: Mayxay
authors.affiliation: Salavan Provincial Hospital, Salavan Provincial Health Department, Salavan Province, Laos.
authors.forename: Sommay
authors.initials: S
authors.lastname: Keomany
authors.affiliation: Salavan Provincial Hospital, Salavan Provincial Health Department, Salavan Province, Laos.
authors.forename: Phoutthalavanh
authors.initials: P
authors.lastname: Souvannasing
authors.affiliation: Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR.|Centre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom.
authors.forename: Paul N
authors.initials: PN
authors.lastname: Newton
authors.affiliation: Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR.|UMR "Emergence des Pathologies Virales" (EPV: Aix-Marseille university - IRD 190 - Inserm 1207 - EHESP), Marseille, France.|Centre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom.
authors.forename: Audrey
authors.identifier: http://orcid.org/0000-0003-1267-0725
authors.initials: A
authors.lastname: Dubot-Pérès
mesh_terms: D000914:Antibodies, Viral / Q000737:chemistry / Q000276:immunology; D003715:Dengue / Q000175:diagnosis* / Q000821:virology; D003716:Dengue Virus / Q000302:isolation & purification* / Q000472:pathogenicity; D003955:Diagnostic Tests, Routine / Q000592:standards*; D005260:Female; D006358:Hot Temperature; D006801:Humans; D007812:Laos; D008297:Male; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D014329:Tropical Climate; D017361:Viral Nonstructural Proteins / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D011933:Reagent Kits, Diagnostic; D017361:Viral Nonstructural Proteins