title: Mathematical Model for Predicting the Growth Probability ofStaphylococcus aureusin Combinations of NaCl and NaNO2under Aerobic or Evacuated Storage Conditions.
abstract: The objective of this study was to describe the growth patterns ofStaphylococcus aureusin combinations of NaCl and NaNO2, using a probabilistic model. A mixture ofS. aureusstrains (NCCP10826, ATCC13565, ATCC14458, ATCC23235, and ATCC27664) was inoculated into nutrient broth plus NaCl (0, 0.25, 0.5, 0.75, 1, 1.25, 1.5, and 1.75%) and NaNO2(0, 15, 30, 45, 60, 75, 90, 105, and 120 ppm). The samples were then incubated at 4, 7, 10, 12 and 15℃ for up to 60 d under aerobic or vacuum conditions. Growth responses [growth (1) or no growth (0)] were then determined every 24 h by turbidity, and analyzed to select significant parameters (p<0.05) by a stepwise selection method, resulting in a probabilistic model. The developed models were then validated with observed growth responses.S. aureusgrowth was observed only under aerobic storage at 10-15℃. At 10-15℃, NaCl and NaNO2did not inhibitS. aureusgrowth at less than 1.25% NaCl. Concentration dependency was observed for NaCl at more than 1.25%, but not for NaNO2. The concordance percentage between observed and predicted growth data was approximately 93.86%. This result indicates thatS. aureusgrowth can be inhibited in vacuum packaging and even aerobic storage below 10℃. Furthermore, NaNO2does not effectively inhibitS. aureusgrowth.
authors.affiliation: Department of Food and Nutrition, Sookmyung Women's University, Seoul 04310, Korea; Risk Analysis Research Center, Sookmyung Women's University, Seoul 04310, Korea.
authors.forename: Jeeyeon
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Food and Nutrition, Sookmyung Women's University, Seoul 04310, Korea.
authors.forename: Eunji
authors.initials: E
authors.lastname: Gwak
authors.affiliation: Department of Food and Nutrition, Sookmyung Women's University, Seoul 04310, Korea; Risk Analysis Research Center, Sookmyung Women's University, Seoul 04310, Korea.
authors.forename: Jimyeong
authors.initials: J
authors.lastname: Ha
authors.affiliation: Department of Food and Nutrition, Sookmyung Women's University, Seoul 04310, Korea; Risk Analysis Research Center, Sookmyung Women's University, Seoul 04310, Korea.
authors.forename: Sejeong
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Food and Nutrition, Sookmyung Women's University, Seoul 04310, Korea; Risk Analysis Research Center, Sookmyung Women's University, Seoul 04310, Korea.
authors.forename: Soomin
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Food and Nutrition, Sookmyung Women's University, Seoul 04310, Korea; Risk Analysis Research Center, Sookmyung Women's University, Seoul 04310, Korea.
authors.forename: Heeyoung
authors.initials: H
authors.lastname: Lee
authors.affiliation: National Institute of Animal Science, RDA, Wanju 55365, Korea.
authors.forename: Mi-Hwa
authors.initials: MH
authors.lastname: Oh
authors.affiliation: National Institute of Animal Science, RDA, Wanju 55365, Korea.
authors.forename: Beom-Young
authors.initials: BY
authors.lastname: Park
authors.affiliation: R&D Center, Seoul Dairy Cooperative, Ansan 15407, Korea.
authors.forename: Nam Su
authors.initials: NS
authors.lastname: Oh
authors.affiliation: Department of Oral Microbiology, College of Dentistry, Wonkwang University, Iksan 54538, Korea.
authors.forename: Kyoung-Hee
authors.initials: KH
authors.lastname: Choi
authors.affiliation: Department of Food and Nutrition, Sookmyung Women's University, Seoul 04310, Korea; Risk Analysis Research Center, Sookmyung Women's University, Seoul 04310, Korea.
authors.forename: Yohan
authors.initials: Y
authors.lastname: Yoon
keywords: NaCl; NaNO2; S. aureus; predictive model; processed meat products