title: Improved Bone Micro Architecture Healing Time after Implant Surgery in an Ovariectomized Rat.
abstract: The present animal study investigated whether oral intake of synthetic bone mineral (SBM) improves peri-implant bone formation and bone micro architecture (BMA). SBM was used as an intervention experimental diet and AIN-93M was used as a control. The SBM was prepared by mixing dicalcium phosphate dihydrate (CaHPO4·2H2O) and magnesium and zinc chlorides (MgCl2and ZnCl2, respectively), and hydrolyzed in double-distilled water containing dissolved potassium carbonate and sodium fluoride. All rats were randomly allocated into one of two groups: a control group was fed without SBM (n = 18) or an experimental group was fed with SBM (n = 18), at seven weeks old. At 9 weeks old, all rats underwent implant surgery on their femurs under general anesthesia. The implant was inserted into the insertion socket prepared at rats' femur to a depth of 2.5 mm by using a drill at 500 rpm. Nine rats in each group were randomly selected and euthanized at 2 weeks after implantation. The remaining nine rats in each group continued their diets, and were euthanized in the same manner at 4 weeks after implantation. The femur, including the implant, was removed from the body and implant was pulled out by an Instron universal testing machine. After the implant removal, BMA was evaluated by bone surface ratio (BS/BV), bone volume fraction (BV/TV), trabecular thickness (TbTh), trabecular number (TbN), trabecular star volume (Vtr), and micro-CT images. BS/BV, BV/TV, TbTh and Vtr were significantly greater in the rats were fed with SBM than those were fed without SBM at 2 and 4 weeks after implantation (P < 0.05). The present results revealed that SBM improves the peri-implant formation and BMA, prominent with trabecular bone structure. The effect of SBM to improve secondary stability of the implant, and shortening the treatment period should be investigated in the future study.
authors.affiliation: Department of Removable Prosthodontics, Nihon University Graduate School of Dentistry at Matsudo, Removable Prosthodontics, Matsudo, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Takahashi
authors.affiliation: Department of Removable Prosthodontics, Nihon University of Dentistry at Matsudo, Matsudo, Japan.
authors.forename: Takehiro
authors.initials: T
authors.lastname: Watanabe
authors.affiliation: Department of Removable Prosthodontics, Nihon University of Dentistry at Matsudo, Matsudo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakada
authors.affiliation: Department of Removable Prosthodontics, Nihon University Graduate School of Dentistry at Matsudo, Removable Prosthodontics, Matsudo, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Sato
authors.affiliation: Department of Dental Biomaterials, Nihon University of Dentistry at Matsudo, Matsudo, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tanimoto
authors.affiliation: Department of Histology, Nihon University of Dentistry at Matsudo, Matsudo, Japan.
authors.forename: Toshiro
authors.initials: T
authors.lastname: Sakae
authors.affiliation: Department of Removable Prosthodontics, Nihon University of Dentistry at Matsudo, Matsudo, Japan.
authors.forename: Suguru
authors.initials: S
authors.lastname: Kimoto
authors.affiliation: Department of Biomaterials and Biomimetics, New York University College of Dentistry, New York, USA.
authors.forename: Dindo
authors.initials: D
authors.lastname: Mijares
authors.affiliation: Department of Biomaterials and Biomimetics, New York University College of Dentistry, New York, USA.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Removable Prosthodontics, Nihon University of Dentistry at Matsudo, Matsudo, Japan.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Kawai
keywords: Animal study; Bone quality; Dietary supplement; Implant