title: First genotype identification of Trichosporon asahii in Sfax, Tunisia.
abstract: OBJECTIVE
  The objectives of our study were species identification and genotyping of Trichosporon isolates collected at the Parasitology and Mycology Laboratory in Sfax, Tunisia.
  
  
  METHODS
  Molecular identification was carried out by analysing the IGS1 regions of the rDNA of 30 Trichosporon isolates.
  
  
  RESULTS
  Trichosporon asahii was the most frequent species detected. Furthermore, four genotypes were identified in Tunisia: 1 (46.4 %), 4 (35.7 %), 7 (14.3 %) and 3 (3.6 %). In vitro antifungal susceptibility testing of the isolates showed that voriconazole exhibited the highest activity.
  
  
  CONCLUSIONS
  This is the first reported study of genotype identification of T. asahii in Tunisia and even in the African continent.
authors.affiliation: Fungal and Parasitic Molecular Biology Laboratory, School of Medicine, University of Sfax, Sfax, Tunisia.
authors.forename: Hayet
authors.initials: H
authors.lastname: Sellami
authors.affiliation: Fungal and Parasitic Molecular Biology Laboratory, School of Medicine, University of Sfax, Sfax, Tunisia.
authors.forename: Houaida
authors.initials: H
authors.lastname: Trabelsi
authors.affiliation: Fungal and Parasitic Molecular Biology Laboratory, School of Medicine, University of Sfax, Sfax, Tunisia.
authors.forename: Sourour
authors.initials: S
authors.lastname: Neji
authors.affiliation: Fungal and Parasitic Molecular Biology Laboratory, School of Medicine, University of Sfax, Sfax, Tunisia.
authors.forename: Imen
authors.initials: I
authors.lastname: Amouri
authors.affiliation: Fungal and Parasitic Molecular Biology Laboratory, School of Medicine, University of Sfax, Sfax, Tunisia.
authors.forename: Fatma
authors.initials: F
authors.lastname: Cheikhrouhou
authors.affiliation: Fungal and Parasitic Molecular Biology Laboratory, School of Medicine, University of Sfax, Sfax, Tunisia.
authors.forename: Fattouma
authors.initials: F
authors.lastname: Makni
authors.affiliation: Fungal and Parasitic Molecular Biology Laboratory, School of Medicine, University of Sfax, Sfax, Tunisia.
authors.forename: Ali
authors.initials: A
authors.lastname: Ayadi
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D021901:DNA, Intergenic / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D058889:Molecular Typing; D016533:Mycological Typing Techniques; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D014250:Trichosporon / Q000145:classification / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D014416:Tunisia; D065819:Voriconazole / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D021901:DNA, Intergenic; D004275:DNA, Ribosomal; D012339:RNA, Ribosomal, 28S; D065819:Voriconazole