title: Interaction of New-Developed TiO₂-Based Photocatalytic Nanoparticles with Pathogenic Microorganisms and Human Dermal and Pulmonary Fibroblasts.
abstract: TiO₂-based photocatalysts were obtained during previous years in order to limit pollution and to ease human daily living conditions due to their special properties. However, obtaining biocompatible photocatalysts is still a key problem, and the mechanism of their toxicity recently received increased attention. Two types of TiO₂ nanoparticles co-doped with 1% of iron and nitrogen (TiO₂-1% Fe-N) atoms were synthesized in hydrothermal conditions at pH of 8.5 (HT1) and 5.5 (HT2), and their antimicrobial activity and cytotoxic effects exerted on human pulmonary and dermal fibroblasts were assessed. These particles exhibited significant microbicidal and anti-biofilm activity, suggesting their potential application for microbial decontamination of different environments. In addition, our results demonstrated the biocompatibility of TiO₂-1% Fe-N nanoparticles at low doses on lung and dermal cells, which may initiate oxidative stress through dose accumulation. Although no significant changes were observed between the two tested photocatalysts, the biological response was cell type specific and time- and dose-dependent; the lung cells proved to be more sensitive to nanoparticle exposure. Taken together, these experimental data provide useful information for future photocatalytic applications in the industrial, food, pharmaceutical, and medical fields.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania. cristinai.nica@gmail.com.
authors.forename: Ionela Cristina
authors.initials: IC
authors.lastname: Nica
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania. miruna.stan@bio.unibuc.ro.
authors.forename: Miruna Silvia
authors.initials: MS
authors.lastname: Stan
authors.affiliation: Department of Botanic-Microbiology, Faculty of Biology, University of Bucharest, 1-3 Aleea Portocalelor, 60101 Bucharest, Romania. bmarcelica@yahoo.com.|Research Institute of the University of Bucharest-ICUB, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania. bmarcelica@yahoo.com.
authors.forename: Marcela
authors.initials: M
authors.lastname: Popa
authors.affiliation: Department of Botanic-Microbiology, Faculty of Biology, University of Bucharest, 1-3 Aleea Portocalelor, 60101 Bucharest, Romania. carmen.chifiriuc@gmail.com.|Research Institute of the University of Bucharest-ICUB, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania. carmen.chifiriuc@gmail.com.
authors.forename: Mariana Carmen
authors.initials: MC
authors.lastname: Chifiriuc
authors.affiliation: Department of Botanic-Microbiology, Faculty of Biology, University of Bucharest, 1-3 Aleea Portocalelor, 60101 Bucharest, Romania. veronica.lazar2009@gmail.com.|Research Institute of the University of Bucharest-ICUB, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania. veronica.lazar2009@gmail.com.
authors.forename: Veronica
authors.initials: V
authors.lastname: Lazar
authors.affiliation: Research Institute of the University of Bucharest-ICUB, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania. gratiela87@gmail.com.
authors.forename: Gratiela G
authors.initials: GG
authors.lastname: Pircalabioru
authors.affiliation: National R&D Institute for Textiles and Leather Bucharest (INCDTP), 16 Lucretiu Patrascanu, 030508 Bucharest, Romania. iuliana.dumitrescu@certex.ro.
authors.forename: Iuliana
authors.initials: I
authors.lastname: Dumitrescu
authors.affiliation: National Research and Development Institute for Textiles and Leather (INCDTP) Leather and Footwear Research Institute (ICPI), 93 Ion Minulescu, 030215 Bucharest, Romania. madalina.fleancu@yahoo.com.
authors.forename: Madalina
authors.initials: M
authors.lastname: Ignat
authors.affiliation: National Institute of Materials Physics (NIMP), Atomistilor 405A, 077125 Bucharest-Magurele, Romania. mfeder@infim.ro.
authors.forename: Marcel
authors.initials: M
authors.lastname: Feder
authors.affiliation: National Institute of Materials Physics (NIMP), Atomistilor 405A, 077125 Bucharest-Magurele, Romania. liviu.tanase@infim.ro.
authors.forename: Liviu Cristian
authors.initials: LC
authors.lastname: Tanase
authors.affiliation: National Institute of Materials Physics (NIMP), Atomistilor 405A, 077125 Bucharest-Magurele, Romania. imercioniu@infim.ro.
authors.forename: Ionel
authors.initials: I
authors.lastname: Mercioniu
authors.affiliation: National Institute of Materials Physics (NIMP), Atomistilor 405A, 077125 Bucharest-Magurele, Romania. diamand@infim.ro.
authors.forename: Lucian
authors.initials: L
authors.lastname: Diamandescu
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania. anca.dinischiotu@bio.unibuc.ro.
authors.forename: Anca
authors.initials: A
authors.lastname: Dinischiotu
mesh_terms: D008841:Actin Cytoskeleton; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001419:Bacteria; D002384:Catalysis; D002470:Cell Survival / Q000187:drug effects; D003102:Colloids; D005347:Fibroblasts / Q000378:metabolism* / Q000382:microbiology*; D006801:Humans; D057446:Hydrodynamics; D015227:Lipid Peroxidation; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D055668:Photochemical Processes; D056951:Photoelectron Spectroscopy; D014025:Titanium / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D003102:Colloids; C009495:titanium dioxide; D014025:Titanium
keywords: antibacterial; lung fibroblasts; photocatalyst; skin; titania