title: Probing Activation and Deactivation of the BRASSINOSTEROID INSENSITIVE1 Receptor Kinase by Immunoprecipitation.
abstract: Brassinosteroids (BRs) are sterol-derived hormones that control plant growth and development. The BR receptor complex is encoded by the BRASSINOSTEROID INSENSITIVE1 (BRI1) and members of the SOMATIC EMBRYOGENESIS RECEPTOR KINASE family. For activation and deactivation, the BR receptor complex uses different posttranslational modifications and recruits various partner proteins. Here, we describe optimized immunoprecipitation protocols and variants for biochemical analyses of posttranslational modifications of BRI1 and of protein-protein interactions.
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), Centre National de la Recherche Scientifique, Commissariat à l'Energie Atomique, Université Paris Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette, France.
authors.forename: Sara
authors.initials: S
authors.lastname: Martins
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), Centre National de la Recherche Scientifique, Commissariat à l'Energie Atomique, Université Paris Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette, France.
authors.forename: Grégory
authors.initials: G
authors.lastname: Vert
authors.affiliation: Laboratoire de Reproduction et Développement des Plantes, Université de Lyon, Ecole Nationale Supérieure de Lyon, Université ClaudeBernard Lyon 1,, Université de Lyon, Ecole Nationale Supérieure de Lyon, Université Claude Bernard Lyon 1, Centre National de la Recherche Scientifique, Institut National de la Recheche Agronomique, 69342, Lyon, France. yvon.jaillais@ens-lyon.fr.
authors.forename: Yvon
authors.initials: Y
authors.lastname: Jaillais
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western / Q000379:methods; D060406:Brassinosteroids / Q000378:metabolism; D004789:Enzyme Activation; D018506:Gene Expression Regulation, Plant; D047468:Immunoprecipitation / Q000379:methods; D010766:Phosphorylation; D030821:Plants, Genetically Modified; D011485:Protein Binding; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D036226:Seedlings / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D054875:Ubiquitination
chemical_list: D029681:Arabidopsis Proteins; D060406:Brassinosteroids; D011494:Protein Kinases; C440035:SERK1 protein, Arabidopsis; C108270:BRI1 protein, Arabidopsis
keywords: BRI1; Brassinosteroids; Immunoprecipitation; Phosphorylation; Protein–protein interaction; Ubiquitination