title: Tissue Proteomics in Vascular Disease.
abstract: In vascular diseases, as in many other pathophysiological conditions, tissue proteins are subjected to a number of different changes such as protein expression, posttranslational modifications, and proteolytic cleavage. For this reason, the study of the tissue proteome is becoming an increasingly important tool in biological and clinical research. In this chapter, we describe in detail the methodology for the analysis of tryptic digested peptides from aortic tissue extracts from mice with the aim to elucidate differences in the proteome between control and case or vascular diseased tissue samples. The method encompasses the analysis of these complex extracts in a single chromatographic run allowing for increased reproducibility. We also outline the main workflow for the processing of the generated data by statistical and bioinformatic analysis.
authors.affiliation: School of Natural Sciences, University of Stirling, Stirling, UK.
authors.forename: Amaya
authors.initials: A
authors.lastname: Albalat
authors.affiliation: School of Natural Sciences, University of Stirling, Stirling, UK.
authors.forename: William
authors.initials: W
authors.lastname: Mullen
authors.affiliation: School of Natural Sciences, University of Stirling, Stirling, UK.
authors.forename: Holger
authors.initials: H
authors.lastname: Husi
authors.affiliation: School of Natural Sciences, University of Stirling, Stirling, UK. Harald.Mischak@glasgow.ac.uk.|Mosaiques Diagnostics GmbH, Hannover, Germany. Harald.Mischak@glasgow.ac.uk.|Institute of Cardiovascular and Medical Sciences, BHF Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, UK. Harald.Mischak@glasgow.ac.uk.
authors.forename: Harald
authors.initials: H
authors.lastname: Mischak
mesh_terms: D000818:Animals; D019295:Computational Biology; D051379:Mice; D010455:Peptides / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry; D014652:Vascular Diseases / Q000378:metabolism*
chemical_list: D010455:Peptides
keywords: FASP method; MS/MS data analysis; Tissue proteomics