title: Detection of virus particles resembling circovirus and porcine circovirus 2a (PCV2a) sequences in feces of dogs.
abstract: During routine electron microscopy of fecal samples from diarrheic dogs dated from 2000 virus particles resembling circovirus in shape and size were detected in two samples (V2177/00; V3374/00). Polymerase chain reaction (PCR) using primers specific for porcine circovirus type 2 (PCV2) amplified DNA recovered from both samples. Sequencing of PCR amplificates (V2177/00) obtained with PCV2-specific primer pairs revealed a genome size of 1768bp. The nucleotide sequence was highly similar (98% nucleotide identity) to the PCV2a reference sequence.
authors.affiliation: Institute of Hygiene and Infectious Diseases of Animals, Justus Liebig University, Frankfurter Straße 89, 35392 Gießen, Germany. Electronic address: Werner.herbst@vetmed.uni-giessen.de.
authors.forename: Werner
authors.initials: W
authors.lastname: Herbst
authors.affiliation: Clinic of Ruminants and Swine, Justus Liebig University, Frankfurter Straße 112, 35392 Gießen, Germany.
authors.forename: Hermann
authors.initials: H
authors.lastname: Willems
mesh_terms: D000818:Animals; D018173:Circoviridae Infections / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D003967:Diarrhea / Q000662:veterinary*; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D005243:Feces / Q000821:virology; D016679:Genome, Viral; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary
keywords: Circovirus; Dog; Electron microscopy; Feces