title: Anticonvulsant effect of sodium cyclamate and propylparaben on pentylenetetrazol-induced seizures in zebrafish.
abstract: Screening for novel anticonvulsant drugs requires appropriate animal seizure models. Zebrafish provide small, accessible, and cost-efficient preclinical models applicable to high-throughput small molecule screening. Based on previous results in rodents, we have here examined the effects of artificial sweetener sodium cyclamate and antimicrobial agent sodium propylparaben on a model of pentylenetetrazole (PTZ)-induced seizures in zebrafish. Sodium cyclamate reduced the bursts of hyperactivity, the spasms, increased the latency to spasms, and the latency to seizure, while propylparaben increased the latency to spasms. The results show the potential of zebrafish to detect novel anticonvulsant compounds while they also demonstrate the ability of two commonly ingested chemical compounds to modify the seizure threshold when were administrated at low concentration.
authors.affiliation: Instituto de Fisiología y Biofísica (IFIBIO-Houssay), Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Antonella
authors.initials: A
authors.lastname: Pisera-Fuster
authors.affiliation: Instituto de Fisiología y Biofísica (IFIBIO-Houssay), Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Sofía
authors.initials: S
authors.lastname: Otero
authors.affiliation: Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina.
authors.forename: Alan
authors.initials: A
authors.lastname: Talevi
authors.affiliation: Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina.
authors.forename: Luis
authors.initials: L
authors.lastname: Bruno-Blanch
authors.affiliation: Instituto de Fisiología y Biofísica (IFIBIO-Houssay), Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Ramón
authors.identifier: 0000-0001-7053-1577
authors.initials: R
authors.lastname: Bernabeu
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology*; D003494:Cyclamates / Q000494:pharmacology*; D004195:Disease Models, Animal; D010226:Parabens / Q000494:pharmacology*; D010433:Pentylenetetrazole / Q000633:toxicity; D011310:Preservatives, Pharmaceutical / Q000009:adverse effects / Q000494:pharmacology; D011930:Reaction Time / Q000187:drug effects; D012640:Seizures / Q000209:etiology / Q000503:physiopathology*; D013549:Sweetening Agents / Q000009:adverse effects / Q000494:pharmacology; D018675:Toxicity Tests / Q000379:methods; D015027:Zebrafish
chemical_list: D000927:Anticonvulsants; D003494:Cyclamates; D010226:Parabens; D011310:Preservatives, Pharmaceutical; D013549:Sweetening Agents; D010433:Pentylenetetrazole; C006068:propylparaben
keywords: animal behavior; anticonvulsant drugs; seizures; zebrafish