title: First isolation and RFLP genotyping of Toxoplasma gondii from crab-eating fox (Cerdocyon thous-Linnaeus, 1766).
abstract: Wild animals may play an important role in the transmission and maintenance of Toxoplasma gondii in the environment. The purpose of the present study was to isolate and genotype T. gondii from a free-ranging crab-eating fox (Cerdocyon thous-Linnaeus, 1766). A crab-eating fox in critical health condition was attended in a veterinary hospital in Recife, Pernambuco State, Brazil. The animal died despite emergency treatment. The brain was collected aseptically and destined for mouse bioassay. One isolate of T. gondii was obtained, and Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) was used to assess genetic variability at 11 markers (SAG1, SAG2, altSAG2, SAG3, BTUB, GRA6, c228, c292, L358, PK1 and APICO). A murine model was used to assess the virulence of the isolate. Using the PCR-RFLP, genotype ToxoDB #13 was identified, which is considered an atypical strain. The isolate was classified as avirulent in the murine model. This is the first study to report T. gondii infection in the crab-eating fox.
authors.affiliation: Department of Veterinary Medicine, Laboratory of Infectious-Contagious Diseases of Domestic Animals, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, 52171-900, Recife, PE, Brazil.
authors.forename: Jonatas Campos
authors.initials: JC
authors.lastname: de Almeida
authors.affiliation: Department of Veterinary Medicine, Laboratory of Infectious-Contagious Diseases of Domestic Animals, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, 52171-900, Recife, PE, Brazil.
authors.forename: Renata Pimentel Bandeira
authors.initials: RPB
authors.lastname: de Melo
authors.affiliation: Department of Veterinary Medicine, Laboratory of Infectious-Contagious Diseases of Domestic Animals, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, 52171-900, Recife, PE, Brazil.
authors.forename: Camila
authors.initials: C
authors.lastname: de Morais Pedrosa
authors.affiliation: Harmonia Veterinary Hospital, Estrada do Encanamento, 52070-000, Recife, PE, Brazil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: da Silva Santos
authors.affiliation: Department of Veterinary Medicine, Laboratory of Animal Protozoology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, PR 445 Km 380, 86057-970, Londrina, PR, Brazil.
authors.forename: Luiz Daniel
authors.initials: LD
authors.lastname: de Barros
authors.affiliation: Department of Veterinary Medicine, Laboratory of Animal Protozoology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, PR 445 Km 380, 86057-970, Londrina, PR, Brazil.
authors.forename: João Luis
authors.initials: JL
authors.lastname: Garcia
authors.affiliation: Department of Veterinary Medicine, Laboratory of Parasitology and Parasitic Diseases, Universidade Federal de Alagoas, Fazenda São Luís, 57000-000, Viçosa, AL, Brazil.
authors.forename: Wagnner José Nascimento
authors.initials: WJN
authors.lastname: Porto
authors.affiliation: Department of Veterinary Medicine, Laboratory of Infectious-Contagious Diseases of Domestic Animals, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, 52171-900, Recife, PE, Brazil. Electronic address: rinaldo.mota@hotmail.com.
authors.forename: Rinaldo Aparecido
authors.initials: RA
authors.lastname: Mota
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000469:parasitology; D001921:Brain / Q000469:parasitology / Q000473:pathology; D001938:Brazil; D046028:Canidae / Q000469:parasitology*; D004252:DNA Mutational Analysis; D004195:Disease Models, Animal; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D051379:Mice; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics*; D014122:Toxoplasma / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D014124:Toxoplasmosis, Animal / Q000469:parasitology* / Q000473:pathology / Q000635:transmission; D014774:Virulence
keywords: Bioassay; Genotyping; Toxoplasmosis; Wild canids