title: Laparotomy Cryoablation in Rabbit VX2 Pancreatic Carcinoma.
abstract: The aim of this study was to establish a suitable rabbit model and select the optimal protocol for laparotomy cryoablation of pancreatic carcinoma.
  
  
  
  VX2 tumor tissues were inoculated into rabbit pancreases to build the pancreatic carcinoma model; then, the tumor-bearing rabbits were randomly divided into 4 groups: control, treatment A (the cryoablated-iceball diameter was bigger than the tumor), treatment B (iceball was as big as the tumor), and treatment C (iceball was smaller than the tumor). Related laboratory tests were conducted, and survival time was recorded.
  
  
  
  The VX2 pancreatic carcinoma model was successfully established, and serum neuron-specific enolase levels increased continuously after inoculation. Compared with controls, rabbits in treatments A and C groups had no significant survival benefit (P > 0.05), but treatment B significantly prolonged the survival time (P < 0.01).
  
  
  
  VX2 pancreatic cancer model was successfully established with neuron-specific enolase as biomarker. Treatment B may be the optimal protocol for pancreatic carcinoma and a new treatment option for patients with unresectable pancreatic cancer.
authors.affiliation: From the *Department of Hematology and Oncology, China-Japan Union Hospital of Jilin University, Changchun; and †Fuda Cancer Hospital, Jinan University School of Medicine (Guangzhou Fuda Cancer Hospital), and ‡Guangzhou Fuda Cancer Institute, Guangzhou, China.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Zhang
authors.forename: Huimin
authors.initials: H
authors.lastname: Tao
authors.forename: Jianying
authors.initials: J
authors.lastname: Zeng
authors.forename: Gang
authors.initials: G
authors.lastname: Fang
authors.forename: Bing
authors.initials: B
authors.lastname: Liang
authors.forename: Liang
authors.initials: L
authors.lastname: Zhou
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Luo
authors.forename: Jian
authors.initials: J
authors.lastname: Shi
authors.forename: Lizhi
authors.initials: L
authors.lastname: Niu
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000601:surgery*; D045744:Cell Line, Tumor; D003452:Cryosurgery / Q000379:methods*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007813:Laparotomy / Q000379:methods*; D010190:Pancreatic Neoplasms / Q000097:blood / Q000473:pathology / Q000601:surgery*; D010751:Phosphopyruvate Hydratase / Q000097:blood; D011817:Rabbits; D011897:Random Allocation; D016019:Survival Analysis
chemical_list: D014408:Biomarkers, Tumor; D010751:Phosphopyruvate Hydratase