title: A traditional Korean multiple herbal formulae (Yuk-Mi-Jihwang-Tang) attenuates acute restraint stress-induced brain tissue oxidation.
abstract: We aimed to evaluate the protective effects of Yuk-Mi-Jihwang-Tang (YJT) against acute restraint stress-induced brain oxidative damage. A water extract of YJT was prepared and subjected to high performance liquid chromatography - diode array detector-mass spectrometry (HPLC-DAD-MS). Thirty-six heads of C57BL/6J male mice (7 weeks) were divided into six groups (n = 6/group). The mice were orally administrated YJT (0, 50, 100, or 200 mg/kg) or vitamin C (100 mg/kg) for 5 consecutive days before 6 h of acute restraint stress. In the brain tissue, lipidperoxidation, antioxidant components, and pro-inflammatory cytokines were measured, and the serum corticosterone level was determined. Acute restraint stress-induced notably increased lipid peroxidation in brain tissues, and pretreatment with YJT showed a significant decreased the lipid peroxidation levels (p< 0.05). The levels of antioxidant components including total glutathione contents, activities of SOD and catalase were remarkably depleted by acute restraint stress, whereas these alterations were significantly restored by treatment with YJT (p< 0.05 or p< 0.01). The restraint stress markedly increased pro-inflammatory cytokines, such as TNF-α and IL-6 in the gene expression and protein levels (p< 0.05 or p< 0.01). Pretreatment with YJT significantly attenuated serum corticosterone (200 mg/kg, p < 0.05). YJT drastically attenuated the levels of 4- HNE, HO-1, Nox 2 and iNOSwhich were elevated during acute restraint stress, whereas the Nrf2 level was increased in brain tissue protein levels. Our data suggest that YJT protects the brain tissue against oxidative damage and regulates stress hormones.
authors.affiliation: a The Graduate School of Alternative Medicine, Kyonggi University , Seoul , Korea.
authors.forename: Hyoung-Il
authors.initials: HI
authors.lastname: Choi
authors.affiliation: b TKM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine , Daejeon , Korea.
authors.forename: Hye-Won
authors.initials: HW
authors.lastname: Lee
authors.affiliation: c Internal Medicine of Physical Constitution and Immunology, Dunsan Oriental Hospital of Daejeon University , Daejeon , Korea.
authors.forename: Tae-Min
authors.initials: TM
authors.lastname: Eom
authors.affiliation: d Department of Beauty and Health-Care , College of Public Health Care, Daejeon University , Daejeon , Korea , and.
authors.forename: Sung-Ah
authors.initials: SA
authors.lastname: Lim
authors.affiliation: e Department of Natural Medicine Resources , Semyung University , Jecheon , Korea.
authors.forename: Hun-Yong
authors.initials: HY
authors.lastname: Ha
authors.affiliation: c Internal Medicine of Physical Constitution and Immunology, Dunsan Oriental Hospital of Daejeon University , Daejeon , Korea.
authors.forename: In-Chan
authors.initials: IC
authors.lastname: Seol
authors.affiliation: c Internal Medicine of Physical Constitution and Immunology, Dunsan Oriental Hospital of Daejeon University , Daejeon , Korea.
authors.forename: Yoon-Sik
authors.initials: YS
authors.lastname: Kim
authors.affiliation: b TKM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine , Daejeon , Korea.
authors.forename: Dal-Seok
authors.initials: DS
authors.lastname: Oh
authors.affiliation: c Internal Medicine of Physical Constitution and Immunology, Dunsan Oriental Hospital of Daejeon University , Daejeon , Korea.
authors.forename: Ho-Ryong
authors.initials: HR
authors.lastname: Yoo
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D001927:Brain Diseases / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006854:Hydrocortisone / Q000097:blood; D007103:Immobilization; D018836:Inflammation Mediators / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008810:Mice, Inbred C57BL; D009410:Nerve Degeneration; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D013315:Stress, Psychological / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D004798:Enzymes; D018836:Inflammation Mediators; D018696:Neuroprotective Agents; C000589716:yukmijihwang-tang; D001205:Ascorbic Acid; D006854:Hydrocortisone
keywords: Antioxidant; brain; corticosterone; herbal medicine; neurodegenerative diseases; oxidative stress