title: Nanotubes impregnated human olfactory bulb neural stem cells promote neuronal differentiation in Trimethyltin-induced neurodegeneration rat model.
abstract: Neural stem cells (NSCs) are multipotent self-renewing cells that could be used in cellular-based therapy for a wide variety of neurodegenerative diseases including Alzheimer's diseases (AD), Parkinson's disease (PD), amyotrophic lateral sclerosis (ALS), and multiple sclerosis (MS). Being multipotent in nature, they are practically capable of giving rise to major cell types of the nervous tissue including neurons, astrocytes, and oligodendrocytes. This is in marked contrast to neural progenitor cells which are committed to a specific lineage fate. In previous studies, we have demonstrated the ability of NSCs isolated from human olfactory bulb (OB) to survive, proliferate, differentiate, and restore cognitive and motor deficits associated with AD, and PD rat models, respectively. The use of carbon nanotubes (CNTs) to enhance the survivability and differentiation potential of NSCs following their in vivo engraftment have been recently suggested. Here, in order to assess the ability of CNTs to enhance the therapeutic potential of human OBNSCs for restoring cognitive deficits and neurodegenerative lesions, we co-engrafted CNTs and human OBNSCs in TMT-neurodegeneration rat model. The present study revealed that engrafted human OBNSCS-CNTs restored cognitive deficits, and neurodegenerative changes associated with TMT-induced rat neurodegeneration model. Moreover, the CNTs seemed to provide a support for engrafted OBNSCs, with increasing their tendency to differentiate into neurons rather than into glia cells. The present study indicate the marked ability of CNTs to enhance the therapeutic potential of human OBNSCs which qualify this novel therapeutic paradigm as a promising candidate for cell-based therapy of different neurodegenerative diseases.
authors.affiliation: Biomedical Research Center, Qatar University, Doha, Qatar.
authors.forename: Hany E
authors.initials: HE
authors.lastname: Marei
authors.affiliation: Department of Cytology and Histology, Faculty of Veterinary Medicine, Aswan University, Aswan, Egypt.
authors.forename: Ahmed A
authors.initials: AA
authors.lastname: Elnegiry
authors.affiliation: Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Adel
authors.initials: A
authors.lastname: Zaghloul
authors.affiliation: Biomedical Research Center, Qatar University, Doha, Qatar.
authors.forename: Asma
authors.initials: A
authors.lastname: Althani
authors.affiliation: Qatar Biobank, Doha, Qatar.
authors.forename: Nahla
authors.initials: N
authors.lastname: Afifi
authors.affiliation: Department of Cytology and Histology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Abd-Elmaksoud
authors.affiliation: Department of Cytology and Histology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Amany
authors.initials: A
authors.lastname: Farag
authors.affiliation: Department of Cytology and Histology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Samah
authors.initials: S
authors.lastname: Lashen
authors.affiliation: Department of Cytology and Histology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Shymaa
authors.initials: S
authors.lastname: Rezk
authors.affiliation: Department of Cytology and Histology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Zeinab
authors.initials: Z
authors.lastname: Shouman
authors.affiliation: Institute of Translational Pharmacology-CNR, Roma, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Cenciarelli
authors.affiliation: Department of Mechanical and Industrial Engineering, Qatar University, Doha, Qatar.
authors.forename: Anwarul
authors.initials: A
authors.lastname: Hasan
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D002478:Cells, Cultured; D003071:Cognition; D004195:Disease Models, Animal; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D008297:Male; D018782:Maze Learning; D008856:Microscopy, Fluorescence; D050997:Nanomedicine / Q000379:methods*; D037742:Nanotubes, Carbon; D009410:Nerve Degeneration; D058953:Neural Stem Cells / Q000378:metabolism / Q000637:transplantation*; D019636:Neurodegenerative Diseases / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D055495:Neurogenesis; D009474:Neurons / Q000378:metabolism / Q000473:pathology*; D009830:Olfactory Bulb / Q000166:cytology*; D010641:Phenotype; D017208:Rats, Wistar; D011930:Reaction Time; D013997:Time Factors; D054457:Tissue Scaffolds; D014162:Transfection; D014220:Trialkyltin Compounds
chemical_list: D037742:Nanotubes, Carbon; D014220:Trialkyltin Compounds; D049452:Green Fluorescent Proteins; C011559:tributyltin
keywords: human olfactory bulb neural stem cells; nanotubes; neurodegeneration rat model; neurodegenerative diseases; trimethyltin