title: Comparative transcriptome analysis of Trueperella pyogenes reveals a novel antimicrobial strategy.
abstract: Trueperella pyogenes is a prevalent opportunistic bacterium that normally causes diverse suppurative lesions, endometritis and pneumonia in various economically important animals. Although the genomic information of this species has been announced, little is known about its functional profiles. In this study, by performing a comparative transcriptome analysis between the highly and moderately virulent T. pyogenes isolates, we found the expression of a LuxR-type DNA-binding response regulator, PloR, was significantly up-regulated in the highly virulent T. pyogenes. Protein crystal structure prediction and primary functional assessment suggested that, the quorum-sensing signal molecules of Gram-negative bacteria such as Pseudomonas aeruginosa and Escherichia coli could significantly inhibit the growth, biofilm production and hemolysis of T. pyogenes by binding to the upstream sensor histidine kinase, PloS. Therefore, the PloS/PlosR two-component regulatory system might dominate the virulence of T. pyogenes. Our findings provide a major advance in understanding the pathogenesis of T. pyogenes, and may shed new light on the development of novel therapeutic strategies to control T. pyogenes infection.
authors.affiliation: Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University, 610064, Chengdu, People's Republic of China.|Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, No. 24 South Section 1, Yihuan Road, 610064, Chengdu, People's Republic of China.
authors.forename: Kelei
authors.initials: K
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University, 610064, Chengdu, People's Republic of China.
authors.forename: Wujiao
authors.initials: W
authors.lastname: Li
authors.affiliation: Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University, 610064, Chengdu, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Huang
authors.affiliation: Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University, 610064, Chengdu, People's Republic of China.
authors.forename: Xuhao
authors.initials: X
authors.lastname: Song
authors.affiliation: Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University, 610064, Chengdu, People's Republic of China.
authors.forename: Xiuyue
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, No. 24 South Section 1, Yihuan Road, 610064, Chengdu, People's Republic of China. bsyue@scu.edu.cn.
authors.forename: Bisong
authors.initials: B
authors.lastname: Yue
mesh_terms: D000191:Actinomycetaceae / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity*; D000193:Actinomycetales Infections / Q000382:microbiology / Q000473:pathology*; D000818:Animals; D000890:Anti-Infective Agents; D018441:Biofilms / Q000254:growth & development; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D004926:Escherichia coli / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D000071677:Histidine Kinase / Q000235:genetics / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000378:metabolism; D053038:Quorum Sensing; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics
chemical_list: D000890:Anti-Infective Agents; D004268:DNA-Binding Proteins; D037521:Virulence Factors; D000071677:Histidine Kinase
keywords: Transcriptome; Trueperella pyogenes; Two-component regulatory system; Virulence