title: 64CuCl2PET/CT imaging of mouse muscular injury induced by electroporation.
abstract: Skeletal muscle injury is common in body injuries suffered in sports and car accidents. Development of new tracers is significant for assessing muscular injury with positron emission tomography/computed tomography (PET/CT) and monitoring repair of muscle injury in response to treatment. Copper is required for wound healing and increased copper ions were detected in the soft tissue of wound in rodents and human. Based on the recent finding of increased64Cu uptake in the traumatic brain injury, this study aimed to explore use of64CuCl2as a radiotracer for molecular imaging of muscular injury using PET/CT. Focally increased64Cu uptake by the injured muscular tissue (5.4 ± 1.2% ID/g) was detected in the C57BL/6 mice with electroporation-induced skeletal muscle injury by PET/CT after intravenous injection of64CuCl2as a tracer, compared to low64Cu uptake associated with muscular inflammation induced by intramuscular injection of lipopolysaccharides (0.82 ± 0.26% ID/g,P< 0.01) or physiological64Cu uptake of the non-injured muscular tissues (0.78 ± 0.20% ID/g,P< 0.01). The findings support further investigation of64CuCl2as a new radiotracer for molecular imaging of skeletal muscle injury using PET/CT.
authors.affiliation: Department of Radiology, University of Texas Southwestern Medical Center Dallas, TX, USA.
authors.forename: Fang
authors.initials: F
authors.lastname: Xie
authors.affiliation: Department of Radiology, University of Texas Southwestern Medical CenterDallas, TX, USA; Department of Nuclear Medicine, West China Hospital, Sichuan UniversityChengdu, Sichuan, China.
authors.forename: Huawei
authors.initials: H
authors.lastname: Cai
authors.affiliation: Department of Radiology, University of Texas Southwestern Medical CenterDallas, TX, USA; Advanced Imaging Research Center, University of Texas Southwestern Medical CenterDallas, TX, USA.
authors.forename: Fangyu
authors.initials: F
authors.lastname: Peng
keywords: Muscle injury; copper metabolism; copper-64 chloride; electroporation; positron emission tomography