title: Contribution of the coelomic epithelial cells specific to the left testis in the chicken embryo.
abstract: The left male gonad in the chicken embryo has a thickened cortical layer, but it eventually becomes flattened after the onset of testicular development. Because the destination of the cortical cells migrating from the left gonad remains unclear, we examined this issue herein.
  
  
  
  The testis-inducing gene doublesex- and mab-3-related transcription factor 1 (DMRT1) was detected in a proportion of the columnar and cubic epithelial cells in the cortex of the left testis as well as Sertoli cells in both testes. Interestingly, some of the DMRT1-expressing cortical cells were contiguous with Sertoli cells in the testis cord. Some cortical cells exhibited a vimentin-positive cytoplasm that was elongated all the way to the medulla. In addition, a desmosome-like structure was observed between the elongated cytoplasm in these cells and the adjacent Sertoli cell. After the organ culture, a few cells labeled with a fluorescent dye that stained only the cortical cells at the beginning of the culture were located in the testis cord of the left testis.
  
  
  
  Some cortical cells expressing DMRT1 were suggested to contribute to the Sertoli cells in the testis cord only after the onset of testicular development and only in the left testis. Developmental Dynamics 246:148-156, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Laboratory of Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Omotehara
authors.affiliation: Laboratory of Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan.
authors.forename: Kiichi
authors.initials: K
authors.lastname: Minami
authors.affiliation: Laboratory of Histophysiology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan.
authors.forename: Youhei
authors.initials: Y
authors.lastname: Mantani
authors.affiliation: Laboratory of Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan.
authors.forename: Yuria
authors.initials: Y
authors.lastname: Umemura
authors.affiliation: Laboratory of Histophysiology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Nishida
authors.affiliation: Laboratory of Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan.
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Hirano
authors.affiliation: Laboratory of Biology, Department of Mathematics and Natural Sciences, Graduate School of Teacher Education, Hyogo University of Teacher Education, Katoh, Hyogo, Japan.
authors.forename: Hidefumi
authors.initials: H
authors.lastname: Yoshioka
authors.affiliation: Laboratory of Histophysiology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kitagawa
authors.affiliation: Laboratory of Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan.
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Yokoyama
authors.affiliation: Laboratory of Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan.
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Hoshi
mesh_terms: D000818:Animals; D002465:Cell Movement; D048430:Cell Shape; D002642:Chick Embryo; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000166:cytology; D008297:Male; D012708:Sertoli Cells / Q000166:cytology; D013737:Testis / Q000166:cytology* / Q000196:embryology; D014157:Transcription Factors / Q000032:analysis; D014746:Vimentin / Q000032:analysis
chemical_list: C110547:DMRT1 protein; D014157:Transcription Factors; D014746:Vimentin
keywords: DMRT1; Sertoli cell; left-right asymmetry; testicular development