title: BrothversusSurface-Grown Cells: Differential Regulation of RsmY/Z Small RNAs inPseudomonas aeruginosaby the Gac/HptB System.
abstract: Two-component systems are capable of profoundly affecting genetic regulation in bacteria by detecting environmental stimuli, allowing them to quickly adapt. InPseudomonas aeruginosa, the small RNAs (sRNAs) RsmY and RsmZ are under the control of the GacS/A system. They have been described as ones of the major key players in the control of planktonic and surface-associated behaviors. Genetic regulation by these sRNAs is achieved by the titration of the negative post-transcriptional regulator RsmA which affects the expression of over 500 genes. There is increasing evidence pinpointing the importance of RsmY and RsmZ in the planktonic-sessileP. aeruginosalifestyles switch control. Using swarming motility as a model, we show here that these sRNA are differentially regulated depending on the selected growth conditions (i.e., planktonicversussurface grown-cells). Also, we report that opposite to planktonically grown cells,rsmZregulation does not implicate the response regulator GacA in swarming cells. Furthermore, we present data indicating that RsmY/Z expression influence swarming motilityviathe protein HptB which acts as a negative regulator of these sRNAs and that they do not strictly converge to RsmA as previously reported.
authors.affiliation: Institut National de la Recherche Scientifique, Institut Armand-Frappier, Laval QC, Canada.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Jean-Pierre
authors.affiliation: Institut National de la Recherche Scientifique, Institut Armand-Frappier, LavalQC, Canada; National Research Council Canada, MontréalQC, Canada.
authors.forename: Julien
authors.initials: J
authors.lastname: Tremblay
authors.affiliation: Institut National de la Recherche Scientifique, Institut Armand-Frappier, Laval QC, Canada.
authors.forename: Eric
authors.initials: E
authors.lastname: Déziel
keywords: HptB; broth-surface; genetic regulation; histidine phosphotransfer protein; small RNAs; surface motility; swarming