title: Preparation of hydrophilic interaction/ion-exchange mixed-mode chromatographic stationary phase with adjustable selectivity by controlling different ratios of the co-monomers.
abstract: Development of mixed-mode chromatography (MMC) stationary phase with adjustable selectivity is beneficial to meet the needs of complex samples. In this work, surface-initiated atom transfer radical polymerization (SI-ATRP) using the mixture of two functional monomers was proposed as a new preparation strategy for MMC stationary phase with adjustable selectivity. The mixture of sodium 4-styrenesulfonate (NASS) and dimethylaminoethyl methacrylate (DMAEMA) underwent SI-ATRP to bond poly(NASS-co-DMAEMA) on the surface of silica to prepare hydrophilic interaction/ion-exchange mixed-mode stationary phase. Various analytes (neutral, acidic, basic analytes and strong polar nucleosides) were employed to investigate the retention behaviors. The influences of water content and pH of the mobile phase on the retention validated the mixed-mode retention mechanisms of HILIC and ion-exchange. The charge and polarity of stationary phase as well as the separation selectivity were conveniently manipulated by the ratio of NASS to DMAEMA monomer, and the use of DMAEMA in the mixture additionally endowed the column with the temperature-responsive characteristics. Moreover, the application of the developed column was demonstrated by the successful separation of nucleosides, β-agonists and safflower injection. In a word, the proposed strategy can be potentially applied in the controllable preparation of MMC stationary phase with adjustable selectivity.
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Key Laboratory of Modern Separation Science in Shaanxi Province, College of Chemistry & Materials Science, Northwest University, Xi'an 710127, China.
authors.forename: Chunmiao
authors.initials: C
authors.lastname: Bo
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Key Laboratory of Modern Separation Science in Shaanxi Province, College of Chemistry & Materials Science, Northwest University, Xi'an 710127, China.
authors.forename: Xiaomeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Key Laboratory of Modern Separation Science in Shaanxi Province, College of Chemistry & Materials Science, Northwest University, Xi'an 710127, China.
authors.forename: Chaozhan
authors.initials: C
authors.lastname: Wang
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Key Laboratory of Modern Separation Science in Shaanxi Province, College of Chemistry & Materials Science, Northwest University, Xi'an 710127, China. Electronic address: ymwei@nwu.edu.cn.
authors.forename: Yinmao
authors.initials: Y
authors.lastname: Wei
mesh_terms: D000318:Adrenergic beta-Agonists / Q000302:isolation & purification; D031187:Carthamus tinctorius / Q000737:chemistry; D002845:Chromatography / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D007475:Ion Exchange Resins / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry; D009705:Nucleosides / Q000302:isolation & purification; D058105:Polymerization; D011108:Polymers / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: D000318:Adrenergic beta-Agonists; D007475:Ion Exchange Resins; D008689:Methacrylates; D009705:Nucleosides; D011108:Polymers; D013451:Sulfonic Acids; D012822:Silicon Dioxide; C077114:styrenesulfonic acid polymer
keywords: Hydrophilic/ion-exchange; Mixed-mode chromatography; Selectivity; Surface-initiated atom transfer radical polymerization