title: Determination of antibiotic resistance genes in relation to phylogenetic background inEscherichia coliisolates from fecal samples of healthy pet cats in Kerman city.
abstract: The aim of this study was to determine antibiotic resistance genes, phylogenetic groups and anti-microbial resistance patterns ofEscherichia coliisolates from fecal samples of healthy pet cats in Kerman city. NinetyE. coliisolates were recovered from obtained rectal swabs. Antibiotic resistance pattern of the isolates against seven selected antibiotic was determined using disc diffusion method. Phylogenetic background of the isolates was determined according to the presence of thechuA,yjaAandTspE4C2markers. Theisolates were examined to determine a selection of antibiotic resistance genes includingtetA, tetB, aadA, sulIanddhfrVby polymerase chain reaction. Forty two isolates (46.6%) were positive at least for one of the examined genes. Phylotyping revealed that the isolates are segregated in phylogenetic groups A (66.7%), B1 (1.2%), B2 (13.4%) and D (18.9%). Among 90 isolates, 26.6% were positive fortetBgene, 10.0% for cqnrSgene, 12.3% forsulIandaadAgenes, 8.9% fortetAand 2.2% fordhfrVgene. None of theE. coliisolates were positive forqnrAandqnrBgenes. Sixteen combination patterns of antibiotic resistance genes were identified which belonged to four phylogroups. Maximum and minimum resistant isolates were recorded against to tetracycline (82.3%) and gentamycin (1.2%), respectively. Fifteen antibiotic resistance patterns were determined in different phylo-genetic groups. In conclusion, feces of healthy pet cat in Kerman could be a source of antibiotic resistantE. coliisolates, whereas these isolates were distributed all over the main phylogroups.
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
authors.forename: Baharak
authors.initials: B
authors.lastname: Akhtardanesh
authors.affiliation: Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Ghanbarpour
authors.affiliation: Graduate Student, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
authors.forename: Sadaf
authors.initials: S
authors.lastname: Ganjalikhani
authors.affiliation: Graduate Student, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
authors.forename: Parisa
authors.initials: P
authors.lastname: Gazanfari
keywords: Antibiotic resistance genes; Cat; Escherichia coli; Phylogenetic group