title: Long noncoding RNA MHENCR promotes melanoma progression via regulating miR-425/489-mediated PI3K-Akt pathway.
abstract: Increasing evidences demonstrated that long noncoding RNAs (lncRNAs) are frequently dysregulated and have critical roles in many tumors. However, the roles and functional mechanisms of lncRNAs in melanoma remain largely unknown. In this study, we identified a novel lncRNA MHENCR which was upregulated in melanoma tissues and further upregulated in metastatic melanoma. Increased expression of MHENCR indicted poor survival of melanoma patients. Functional experiments revealed that MHENCR knockdown significantly inhibited melanoma cells proliferation, induced cell cycle arrest and apoptosis, and also attenuated melanoma cells migrationin vitro. Furthermore, we identified MHENCR as a competitively endogenous RNA, which specifically bound to miR-425 and miR-489, upregulated their target genes IGF1 and SPIN1 expression, and further activated PI3K-Akt pathway. Statistically significant correlations were observed between MHENCR expression and IGF1 and SPIN1 in melanoma tissues.In vivofunctional experiments further confirmed the pro-growth and pro-metastasis roles of MHENCR. Collectively, our findings revealed that MHENCR functions as an oncogene in melanoma via activating miR-425/489-mediated PI3K-Akt pathway, and may be a therapeutic target for melanoma.
authors.affiliation: Department of Burn and Plastic Surgery, The 253rd Hospital of PLA Hohhot, Inner Mongolia, China.
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of General Surgery, The 253rd Hospital of PLA Hohhot, Inner Mongolia, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Dong
authors.affiliation: Department of Burn and Plastic Surgery, The 253rd Hospital of PLA Hohhot, Inner Mongolia, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Liu
authors.affiliation: Intensive Care Unit, The 253rd Hospital of PLA Hohhot, Inner Mongolia, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Burn and Plastic Surgery, The 253rd Hospital of PLA Hohhot, Inner Mongolia, China.
authors.forename: Dexiong
authors.initials: D
authors.lastname: Yan
authors.affiliation: Department of Burn and Plastic Surgery, The 253rd Hospital of PLA Hohhot, Inner Mongolia, China.
authors.forename: Xingwei
authors.initials: X
authors.lastname: Yao
authors.affiliation: Department of Burn and Plastic Surgery, The 253rd Hospital of PLA Hohhot, Inner Mongolia, China.
authors.forename: Weijing
authors.initials: W
authors.lastname: Sun
authors.affiliation: Department of Burn and Plastic Surgery, The 253rd Hospital of PLA Hohhot, Inner Mongolia, China.
authors.forename: Dezhi
authors.initials: D
authors.lastname: Han
authors.affiliation: Department of Burn and Plastic Surgery, The 253rd Hospital of PLA Hohhot, Inner Mongolia, China.
authors.forename: Guozhen
authors.initials: G
authors.lastname: Gao
keywords: Melanoma; PI3K-Akt pathway; competing endogenous RNA; long noncoding RNA; miR-425; miR-489