title: The effect of aloe vera on the expression of wound healing factors (TGFβ1 and bFGF) in mouse embryonic fibroblast cell: In vitro study.
abstract: BACKGROUND
  Aloe vera (A.v) have been used traditionally for topical treatment of wounds and burns in different countries for centuries, but the mechanism of this effect is not well understood. Various growth factors are implicated in the process of wound healing. Among the different growth factors involved in the process, TGFβ1 and bFGF are the most importantly expressed in fibroblast cells. The aim of this study was to evaluate the effect of A.v on the expression of angiogenesis growth factors in mouse embryonic fibroblast cells.
  
  
  METHODS
  We exposed mouse embryonic fibroblast cells to different concentrations of A.v (50, 100 and 150μg/ml) at two different time of 12 and 24h. Fibroblast cell without A.v treatment serves as the control. The expression of TGFβ1and bFGF was measured by real time-polymerase chain reaction (real-time-PCR) and enzyme-linked immunosorbent assay (ELISA) at the level of gene and protein.
  
  
  RESULTS
  We observed that A.v gel at first up-regulated the expression of TGFβ1 and bFGF, but, these genes were later repressed after a particular time.
  
  
  CONCLUSIONS
  Our results demonstrated that A.v was dose-dependent and time-dependent on the expression of bFGF and TGFβ1 in fibroblast cell in vitro. This mechanism can be employed in the prospective treatment of physical lesion.
authors.affiliation: Razi Herbal Researches Center, Lorestan University of Medical Sciences, Khoramabad, Iran; Department of Biochemistry, Lorestan University of Medical Science, Khorramabad, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Hormozi
authors.affiliation: Department of Physiology, Lorestan University of Medical Science, Khorramabad, Iran.
authors.forename: Raheleh
authors.initials: R
authors.lastname: Assaei
authors.affiliation: Department of Anatomical Sciences, Lorestan University of Medical Science, Khorramabad, Iran. Electronic address: a-eatemadi@razi.tums.ac.ir.
authors.forename: Mandana Beigi
authors.initials: MB
authors.lastname: Boroujeni
mesh_terms: D000504:Aloe / Q000737:chemistry*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004622:Embryo, Mammalian / Q000166:cytology*; D016222:Fibroblast Growth Factor 2 / Q000235:genetics* / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction; D053773:Transforming Growth Factor beta1 / Q000235:genetics* / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D010936:Plant Extracts; D053773:Transforming Growth Factor beta1; D016222:Fibroblast Growth Factor 2
keywords: Aloe vera (A.v); Fibroblast; TGFβ1; bFGF