title: Dynamic imaging of MYC and CDKN1A mRNAs as an indicator of cell G1-phase arrest.
abstract: The identification of G1-phase arrest requires a dynamic capturing method. In this work, we reported an indicator based on MYC and CDKN1A mRNA imaging to visualize G1-phase cycle arrest. A typical G1-phase arrest imaging is shown as a green-to-red conversion by fluorescence molecular beacons indicating the change as down-regulating of MYC and up-regulating of CDKN1A mRNA. We performed G1-phase arrest imaging on proliferated cancer cells inhibited by TMPyP4 and explore the role of HIF-1α in the proliferation inhibition effect.
authors.affiliation: School of Science, Beijing University of Chemical Technology, Beijing 100029, China and Department of Chemistry, Beijing Key Laboratory of Micronalytical Methods and Instrumentation, The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing 100084, China. jmlin@mail.tsinghua.edu.cn.
authors.forename: Linglu
authors.identifier: http://orcid.org/0000-0002-7801-7385
authors.initials: L
authors.lastname: Yi
authors.affiliation: School of Science, Beijing University of Chemical Technology, Beijing 100029, China and Department of Chemistry, Beijing Key Laboratory of Micronalytical Methods and Instrumentation, The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing 100084, China. jmlin@mail.tsinghua.edu.cn.
authors.forename: Xuexia
authors.initials: X
authors.lastname: Lin
authors.affiliation: Department of Chemistry, Beijing Key Laboratory of Micronalytical Methods and Instrumentation, The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing 100084, China. jmlin@mail.tsinghua.edu.cn.
authors.forename: Haifang
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Chemistry, Beijing Key Laboratory of Micronalytical Methods and Instrumentation, The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing 100084, China. jmlin@mail.tsinghua.edu.cn.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Chemistry, Beijing Key Laboratory of Micronalytical Methods and Instrumentation, The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing 100084, China. jmlin@mail.tsinghua.edu.cn.
authors.forename: Jin-Ming
authors.identifier: http://orcid.org/0000-0001-8891-0655
authors.initials: JM
authors.lastname: Lin
mesh_terms: D059447:Cell Cycle Checkpoints / Q000187:drug effects* / Q000235:genetics*; D049109:Cell Proliferation / Q000187:drug effects; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics*; D005453:Fluorescence; D016193:G1 Phase / Q000187:drug effects* / Q000235:genetics*; D006801:Humans; D061986:MCF-7 Cells; D057054:Molecular Imaging / Q000379:methods*; D017319:Photosensitizing Agents / Q000494:pharmacology; D011166:Porphyrins / Q000494:pharmacology; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*
chemical_list: C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D017319:Photosensitizing Agents; D011166:Porphyrins; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; C021096:tetra(4-N-methylpyridyl)porphine