title: A comprehensive experiment for molecular biology: Determination of single nucleotide polymorphism in human REV3 gene using PCR-RFLP.
abstract: Laboratory exercise is helpful for medical students to understand the basic principles of molecular biology and to learn about the practical applications of molecular biology. We have designed a lab course on molecular biology about the determination of single nucleotide polymorphism (SNP) in human REV3 gene, the product of which is a subunit of DNA polymerase ζ and SNPs in this gene are associated with altered susceptibility to cancer. This newly designed experiment is composed of three parts, including genomic DNA extraction, gene amplification by PCR, and genotyping by RFLP. By combining these activities, the students are not only able to learn a series of biotechniques in molecular biology, but also acquire the ability to link the learned knowledge with practical applications. This comprehensive experiment will help the medical students improve the conceptual understanding of SNP and the technical understanding of SNP detection. © 2017 by The International Union of Biochemistry and Molecular Biology, 45(4):299-304, 2017.
authors.affiliation: Department of Molecular Biology and Diagnostics, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, China.|Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, Zhenjiang, Jiangsu, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, Zhenjiang, Jiangsu, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Shao
authors.affiliation: Experimental Teaching Center, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Gao
authors.affiliation: Experimental Teaching Center, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Molecular Biology and Diagnostics, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, China.
authors.forename: Zixuan
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Department of Molecular Biology and Diagnostics, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Molecular Biology and Diagnostics, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, China.|Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, Zhenjiang, Jiangsu, China.
authors.forename: Yongmin
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, Zhenjiang, Jiangsu, China.
authors.forename: Qixiang
authors.initials: Q
authors.lastname: Shao
authors.affiliation: Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, Zhenjiang, Jiangsu, China.
authors.forename: Wenrong
authors.initials: W
authors.lastname: Xu
authors.affiliation: Department of Molecular Biology and Diagnostics, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, China.|Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, Zhenjiang, Jiangsu, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Qian
mesh_terms: D004247:DNA / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D004521:Educational Measurement / Q000706:statistics & numerical data*; D004587:Electrophoresis, Agar Gel; D015870:Gene Expression; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000378:metabolism; D008967:Molecular Biology / Q000193:education*; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D013334:Students
chemical_list: D017931:DNA Primers; D004268:DNA-Binding Proteins; D004247:DNA; D004259:DNA-Directed DNA Polymerase; C118285:REV3L protein, human
keywords: Laboratory exercise; PCR-RFLP; SNP; molecular biology