title: Premature remodeling of fat body and fat mobilization triggered by platelet-derived growth factor/VEGF receptor inDrosophila.
abstract: InDrosophila,fat-body remodeling accompanied with fat mobilization is an ecdysone-induced dynamic process that only occurs during metamorphosis. Here, we show that the activatedDrosophilaplatelet-derived growth factor/VEGF receptor (PVR) is sufficient to induce shape changes in the fat body, from thin layers of tightly conjugated polygonal cells to clusters of disaggregated round-shaped cells. These morphologic changes are reminiscent of those seen during early pupation upon initiation of fat-body remodeling. Activation of PVR also triggers an early onset of lipolysis and mobilization of internal storage, as revealed by the appearance of small lipid droplets and up-regulated lipolysis-related genes. We found that PVR displays a dynamic expression pattern in the fat body and peaks at the larval-prepupal transition under the control of ecdysone signaling. Removal of PVR, although it does not prevent ecdysone-induced fat-body remodeling, causes ecdysone signaling to be up-regulated. Our data reveal that PVR is active in a dual-secured mechanism that involves an ecdysone-induced fat-body remodeling pathway and a reinforced PVR pathway for effective lipid mobilization. Ectopic expression of activated c-kit-the mouse homolog of PVR in theDrosophilafat body-also results in a similar phenotype. This may suggest a novel function of c-kit as it relates to lipid metabolism in mammals.-Zheng, H., Wang, X., Guo, P., Ge, W., Yan, Q., Gao, W., Xi, Y., Yang, X. Premature remodeling of fat body and fat mobilization triggered by platelet-derived growth factor/VEGF receptor inDrosophila.
authors.affiliation: Division of Human Reproduction and Developmental Genetics, The Women's Hospital, and.|Department of Genetics, Zhejiang University School of Medicine, Hangzhou, China.|Institute of Genetics and.|College of Life Sciences, Zhejiang University, Hangzhou, China.
authors.forename: Huimei
authors.initials: H
authors.lastname: Zheng
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, China.
authors.forename: Xuexiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Division of Human Reproduction and Developmental Genetics, The Women's Hospital, and.|Department of Genetics, Zhejiang University School of Medicine, Hangzhou, China.|Institute of Genetics and.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Guo
authors.affiliation: Division of Human Reproduction and Developmental Genetics, The Women's Hospital, and.|Department of Genetics, Zhejiang University School of Medicine, Hangzhou, China.|Institute of Genetics and.
authors.forename: Wanzhong
authors.initials: W
authors.lastname: Ge
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, China.
authors.forename: Qinfeng
authors.initials: Q
authors.lastname: Yan
authors.affiliation: School of Biomedical Engineering and.|Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China; and.
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Gao
authors.affiliation: Division of Human Reproduction and Developmental Genetics, The Women's Hospital, and xyyongm@zju.edu.cn.|Department of Genetics, Zhejiang University School of Medicine, Hangzhou, China.|Institute of Genetics and.
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Xi
authors.affiliation: Division of Human Reproduction and Developmental Genetics, The Women's Hospital, and xhyang@zju.edu.cn.|Department of Genetics, Zhejiang University School of Medicine, Hangzhou, China.|Institute of Genetics and.|Joint Institute of Genetics and Genomic Medicine, Zhejiang University-University of Toronto, Zhejiang University, Hangzhou, China.
authors.forename: Xiaohang
authors.initials: X
authors.lastname: Yang
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D005216:Fat Body / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D017344:Genes, Insect / Q000235:genetics; D008675:Metamorphosis, Biological / Q000502:physiology; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation / Q000235:genetics
chemical_list: D029721:Drosophila Proteins; D019009:Proto-Oncogene Proteins c-kit; C438368:Pvr protein, Drosophila; D020794:Receptor Protein-Tyrosine Kinases
keywords: CD117/c-kit; Rac1; ecdysone; fat cell; lipid metabolism