title: Aloperine attenuates hydrogen peroxide-induced injury via anti-apoptotic activity and suppression of the nuclear factor-κB signaling pathway.
abstract: Aloperine is an alkaloid that exerts significant inhibitive effects on acute inflammation and Type III and IV hypersensitivity caused by a variety of inflammatory agents. The aims of the present study were to investigate whether the protective effect of aloperine attenuates hydrogen peroxide (H2O2)-induced injury, and to identify the underlying mechanisms involved. Nucleus pulposus cells were extracted from adult male Sprague-Dawley rats, and incubated with fresh medium containing 200 µM H2O2for 24 h. In the study, treatment with aloperine significantly increased cell viability and suppressed apoptosis in H2O2-treated nucleus pulposus cells in a dose-dependent manner. In addition, 10 and 100 nM aloperine significantly inhibited H2O2-induced tumor necrosis factor-α and interleukin-6 activities, and significantly increased the H2O2-reduced superoxide dismutase and glutathione peroxidase activities in nucleus pulposus cells (all P<0.01). However, aloperine treatment (10 and 100 nM) significantly reduced the H2O2-induced caspase-9 activity in nucleus pulposus cells. Furthermore, addition of 10 and 100 nM aloperine significantly suppressed nuclear factor-κB (NF-κB) and phosphorylated-protein kinase B expression levels in H2O2-treated nucleus pulposus cells. In conclusion, the protective effect of aloperine attenuated H2O2-induced injury via hyperproliferation, its anti-apoptotic activity and suppression of the NF-κB signaling pathway.
authors.affiliation: Department of Orthopedics, The First Central Hospital of Baoding, Baoding, Hebei 071000, P.R. China.
authors.forename: Dongliang
authors.initials: D
authors.lastname: Ren
authors.affiliation: Department of Orthopedics, The First Central Hospital of Baoding, Baoding, Hebei 071000, P.R. China.
authors.forename: Weisong
authors.initials: W
authors.lastname: Ma
authors.affiliation: Department of Orthopedics, The First Central Hospital of Baoding, Baoding, Hebei 071000, P.R. China.
authors.forename: Baozhen
authors.initials: B
authors.lastname: Guo
authors.affiliation: Department of Orthopedics, The First Central Hospital of Baoding, Baoding, Hebei 071000, P.R. China.
authors.forename: Shunyi
authors.initials: S
authors.lastname: Wang
keywords: aloperine; anti-apoptotic; hydrogen peroxide-induced nucleus pulposus cell; nuclear factor-κB