title: A UPLC-MS/MS method for analysis of vancomycin in human cerebrospinal fluid and comparison with the chemiluminescence immunoassay.
abstract: Vancomycin (VCM) is clinically used in treating patients with postoperative intracranial infections. The cerebrospinal fluid (CSF) concentration of VCM varies greatly among patients. To guide the dosage regimens, monitoring of VCM in CSF is needed. However a method for analysis of VCM in human CSF is lacking. An ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed and validated for analysis of VCM in human CSF, and the agreement of UPLC-MS/MS and chemiluminescence immunoassay (CLIA) in the analysis of CSF VCM was evaluated. The ion transitions were m/z 725.5 > 144.1 for VCM and m/z 455.2 > 308.2 for methotrexate (internal standard). The agreement between UPLC-MS/MS and CLIA was evaluated by Bland-Altman plot in 179 samples. The calibration range of the UPLC-MS/MS method was 1-400 mg/L. The inaccuracy and imprecision were -0.69-10.80% and <4.95%. The internal standard normalized recovery and matrix factor were 86.14-99.31 and 85.84-92.07%, respectively. The measurements of CLIA and UPLC-MS/MS were strongly correlated (r > 0.98). The 95% limit of agreement of the ratio of CLIA to UPLC-MS/MS was 61.66-107.40%. Further studies are warranted to confirm the results.
authors.affiliation: Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing, People's Republic of China.|Department of Clinical Pharmacology, College of Pharmaceutical Sciences, Capital Medical University, Beijing, People's Republic of China.
authors.forename: Shenghui
authors.initials: S
authors.lastname: Mei
authors.affiliation: Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing, People's Republic of China.
authors.forename: Jiaqing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing, People's Republic of China.
authors.forename: Leting
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing, People's Republic of China.|Department of Clinical Pharmacology, College of Pharmaceutical Sciences, Capital Medical University, Beijing, People's Republic of China.
authors.forename: Ruiling
authors.initials: R
authors.lastname: Chen
authors.affiliation: Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing, People's Republic of China.|Department of Clinical Pharmacology, College of Pharmaceutical Sciences, Capital Medical University, Beijing, People's Republic of China.
authors.forename: Xingang
authors.initials: X
authors.lastname: Li
authors.affiliation: Intensive Care Unit, Beijing Tiantan Hospital, Capital Medical University, Beijing, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Chen
authors.affiliation: Intensive Care Unit, Beijing Tiantan Hospital, Capital Medical University, Beijing, People's Republic of China.
authors.forename: Guangqiang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Intensive Care Unit, Beijing Tiantan Hospital, Capital Medical University, Beijing, People's Republic of China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Intensive Care Unit, Beijing Tiantan Hospital, Capital Medical University, Beijing, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing, People's Republic of China.|Department of Clinical Pharmacology, College of Pharmaceutical Sciences, Capital Medical University, Beijing, People's Republic of China.
authors.forename: Zhigang
authors.identifier: http://orcid.org/0000-0003-0281-2259
authors.initials: Z
authors.lastname: Zhao
mesh_terms: D000900:Anti-Bacterial Agents / Q000134:cerebrospinal fluid*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016903:Drug Monitoring / Q000379:methods; D006801:Humans; D007118:Immunoassay; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014640:Vancomycin / Q000134:cerebrospinal fluid*
chemical_list: D000900:Anti-Bacterial Agents; D014640:Vancomycin
keywords: UPLC-MS/MS; cerebrospinal fluid; chemiluminescence immunoassay; method comparison; method development and validation; vancomycin