title: Chemical probing of thiotetronate bio-assembly.
abstract: Chemical 'chain termination' probes were utilised for the investigation of thiotetronate antibiotic biosynthesis in the filamentous bacteria Lentzea sp. and Streptomyces thiolactonus NRRL 15439. The use of these tools led to the capture of biosynthetic intermediates involved in the thiotetronate polyketide backbone assembly, providing first insights into substrate specificity and in vivo intermediate processing by unusual iterative synthases.
authors.affiliation: Department of Chemistry, University of Warwick, Library Road, CV4 7AL, UK. M.Tosin@warwick.ac.uk.
authors.forename: Judith
authors.initials: J
authors.lastname: Havemann
authors.affiliation: Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK.
authors.forename: Marie E
authors.initials: ME
authors.lastname: Yurkovich
authors.affiliation: Department of Chemistry, University of Warwick, Library Road, CV4 7AL, UK. M.Tosin@warwick.ac.uk.
authors.forename: Robert
authors.initials: R
authors.lastname: Jenkins
authors.affiliation: Department of Chemistry, University of Warwick, Library Road, CV4 7AL, UK. M.Tosin@warwick.ac.uk.
authors.forename: Sophia
authors.initials: S
authors.lastname: Harringer
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, People's Republic of China.
authors.forename: Weixin
authors.initials: W
authors.lastname: Tao
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, People's Republic of China.
authors.forename: Shishi
authors.initials: S
authors.lastname: Wen
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, People's Republic of China.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK.
authors.forename: Peter F
authors.initials: PF
authors.lastname: Leadlay
authors.affiliation: Department of Chemistry, University of Warwick, Library Road, CV4 7AL, UK. M.Tosin@warwick.ac.uk.
authors.forename: Manuela
authors.identifier: http://orcid.org/0000-0003-4340-9431
authors.initials: M
authors.lastname: Tosin
mesh_terms: D039903:Actinobacteria / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D008968:Molecular Conformation; D015335:Molecular Probes / Q000032:analysis* / Q000737:chemistry*; D048630:Polyketide Synthases / Q000378:metabolism; D013302:Streptomyces / Q000378:metabolism; D013379:Substrate Specificity; D013438:Sulfhydryl Compounds / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D015335:Molecular Probes; D013438:Sulfhydryl Compounds; D048630:Polyketide Synthases