title: Oat Doubled Haploids Following Maize Pollination.
abstract: Doubled haploids (DHs) are an important tool for the accelerated production of new crop varieties. In oat, DHs were first produced by pollinating oat florets with maize pollen. The resultant embryos spontaneously eliminate the maize chromosomes leaving a haploid complement of oat chromosomes. These embryos can be cultured in vitro using the "embryo rescue" technique to produce haploid plants whose chromosome number can be doubled with colchicine to produce homozygous DH oat plants.
authors.affiliation: South Australian Research and Development Institute, GPO Box 397, Adelaide, SA, 5001, Australia. Phil.Davies@sa.gov.au.
authors.forename: Philip A
authors.initials: PA
authors.lastname: Davies
authors.affiliation: South Australian Research and Development Institute, GPO Box 397, Adelaide, SA, 5001, Australia.
authors.forename: Parminder K
authors.initials: PK
authors.lastname: Sidhu
mesh_terms: D018554:Avena / Q000235:genetics* / Q000254:growth & development; D001947:Breeding; D003433:Crosses, Genetic; D006238:Haploidy; D010641:Phenotype; D054817:Pollination; D012639:Seeds / Q000235:genetics; D003313:Zea mays / Q000235:genetics*
keywords: Avena sativa; Breeding; Doubled haploid; Haploid; Maize; Oat; Zea mays