title: Improved method for the detection of catheter colonization and catheter-related bacteremia in newborns.
abstract: Accurate diagnosis of catheter-related bloodstream infection (CRBSI) is mandatory for hospital infection control. Peripherally inserted central venous catheters (PICCs) are widely used in intensive care units, but studies about procedures for detection of colonization are scarce in neonates. We sequentially processed 372 PICCs by 2 methods, first by the standard roll-plate (RP) technique and then by rubbing catheters on a blood agar plate after being longitudinally split (LS). With both techniques, we detected 133 colonized PICCs. Ninety-four events of CRBSI were diagnosed. The sensitivity, specificity, positive predictive value, and negative predictive value for detection of CRBSI were 58.5%, 92.8%, 73.3%, and 86.9%, respectively, for RP technique and 96.8%, 88.5%, 74.0%, and 98.8%, respectively, for LS technique. The LS technique increased the proportion of detected CRBSI by 38.3%. Neonatal PICC tips should be cultured after cutting them open. This technique is simple and sensitive to detect catheter colonization and also to diagnose CRBSI.
authors.affiliation: Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid 28007, Spain; Department of Medicine, School of Medicine, Universidad Complutense de Madrid, Madrid 28040, Spain; CIBER Enfermedades Respiratorias (CB06/06/0058), Madrid 28029, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Martín-Rabadán
authors.affiliation: Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid 28007, Spain. Electronic address: felipe.perez.garcia.87@gmail.com.
authors.forename: F
authors.initials: F
authors.lastname: Pérez-García
authors.affiliation: Neonatology Unit, Hospital General Universitario Gregorio Marañón, Madrid 28007, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Zamora Flores
authors.affiliation: Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid 28007, Spain.
authors.forename: E S
authors.initials: ES
authors.lastname: Nisa
authors.affiliation: Instituto de Investigación Sanitaria Hospital Gregorio Marañón, Madrid 28007, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Guembe
authors.affiliation: Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid 28007, Spain; Department of Medicine, School of Medicine, Universidad Complutense de Madrid, Madrid 28040, Spain; CIBER Enfermedades Respiratorias (CB06/06/0058), Madrid 28029, Spain; Instituto de Investigación Sanitaria Hospital Gregorio Marañón, Madrid 28007, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Bouza
mesh_terms: D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology*; D055499:Catheter-Related Infections / Q000175:diagnosis* / Q000382:microbiology*; D002405:Catheterization, Central Venous / Q000009:adverse effects*; D002408:Catheters, Indwelling / Q000382:microbiology; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007362:Intensive Care Units; D008297:Male; D008828:Microbiological Techniques / Q000379:methods*; D012189:Retrospective Studies; D012680:Sensitivity and Specificity
keywords: Catheter-related infections; Diagnosis; Epidemiology; Microbiology; Peripherally inserted central catheters