title: Genetic dissection of powdery mildew resistance in interspecific half-sib grapevine families using SNP-based maps.
abstract: Quantitative trait locus (QTL) identification in perennial fruit crops is impeded largely by their lengthy generation time, resulting in costly and labor-intensive maintenance of breeding programs. In a grapevine (genusVitis) breeding program, although experimental families are typically unreplicated, the genetic backgrounds may contain similar progenitors previously selected due to their contribution of favorable alleles. In this study, we investigated the utility of joint QTL identification provided by analyzing half-sib families. The genetic control of powdery mildew was studied using two half-sib F1families, namely GE0711/1009 (MN1264 × MN1214;N= 147) and GE1025 (MN1264 × MN1246;N= 125) with multiple species in their ancestry. Maternal genetic maps consisting of 1077 and 1641 single nucleotide polymorphism (SNP) markers, respectively, were constructed using a pseudo-testcross strategy. Ratings of field resistance to powdery mildew were obtained based on whole-plant evaluation of disease severity. This 2-year analysis uncovered two QTLs that were validated on a consensus map in these half-sib families with improved precision relative to the parental maps. Examination of haplotype combinations based on the two QTL regions identified strong association of haplotypes inherited from 'Seyval blanc', through MN1264, with powdery mildew resistance. This investigation also encompassed the use of microsatellite markers to establish a correlation between 206-bp (UDV-015b) and 357-bp (VViv67) fragment sizes with resistance-carrying haplotypes. Our work is one of the first reports in grapevine demonstrating the use of SNP-based maps and haplotypes for QTL identification and tagging of powdery mildew resistance in half-sib families.
authors.affiliation: Department of Horticultural Science, University of Minnesota, Saint Paul, MN 55108 USA.
authors.forename: Soon Li
authors.initials: SL
authors.lastname: Teh
authors.affiliation: Department of Horticulture and Crop Science, The Ohio State University/OARDC, Wooster, OH 44691 USA.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Fresnedo-Ramírez
authors.affiliation: Department of Horticultural Science, University of Minnesota, Saint Paul, MN 55108 USA.
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Clark
authors.affiliation: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva, NY 14456 USA.
authors.forename: David M
authors.initials: DM
authors.lastname: Gadoury
authors.affiliation: BRC Bioinformatics Facility, Institute of Biotechnology, Cornell University, Ithaca, NY 14853 USA.
authors.forename: Qi
authors.initials: Q
authors.lastname: Sun
authors.affiliation: USDA-ARS Grape Genetics Research Unit, Geneva, NY 14456 USA.
authors.forename: Lance
authors.initials: L
authors.lastname: Cadle-Davidson
authors.affiliation: Department of Horticultural Science, University of Minnesota, Saint Paul, MN 55108 USA.
authors.forename: James J
authors.initials: JJ
authors.lastname: Luby
keywords: Grapevine; Half-sib progeny; Interspecific; Powdery mildew; Pseudo-testcross; QTL