title: Regulators of Lysosome Function and Dynamics inCaenorhabditis elegans.
abstract: Lysosomes, the major membrane-bound degradative organelles, have a multitude of functions in eukaryotic cells. Lysosomes are the terminal compartments in the endocytic pathway, though they display highly dynamic behaviors, fusing with each other and with late endosomes in the endocytic pathway, and with the plasma membrane during regulated exocytosis and for wound repair. After fusing with late endosomes, lysosomes are reformed from the resulting hybrid organelles through a process that involves budding of a nascent lysosome, extension of the nascent lysosome from the hybrid organelle, while remaining connected by a membrane bridge, and scission of the membrane bridge to release the newly formed lysosome. The newly formed lysosomes undergo cycles of homotypic fusion and fission reactions to form mature lysosomes. In this study, we used a forward genetic screen inCaenorhabditis elegansto identify six regulators of lysosome biology. We show that these proteins function in different steps of lysosome biology, regulating lysosome formation, lysosome fusion, and lysosome degradation.
authors.affiliation: Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721.
authors.forename: Kevin
authors.initials: K
authors.lastname: Gee
authors.affiliation: Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721.
authors.forename: Danniel
authors.initials: D
authors.lastname: Zamora
authors.affiliation: Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721.
authors.forename: Teresa
authors.initials: T
authors.lastname: Horm
authors.affiliation: Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721.
authors.forename: Laeth
authors.initials: L
authors.lastname: George
authors.affiliation: Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721.
authors.forename: Cameron
authors.initials: C
authors.lastname: Upchurch
authors.affiliation: Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721.
authors.forename: Justin
authors.initials: J
authors.lastname: Randall
authors.affiliation: Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721.
authors.forename: Colby
authors.initials: C
authors.lastname: Weaver
authors.affiliation: Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721.
authors.forename: Caitlin
authors.initials: C
authors.lastname: Sanford
authors.affiliation: Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721.
authors.forename: Austin
authors.initials: A
authors.lastname: Miller
authors.affiliation: Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Hernandez
authors.affiliation: Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721.
authors.forename: Hope
authors.initials: H
authors.lastname: Dang
authors.affiliation: Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721 fares@email.arizona.edu.
authors.forename: Hanna
authors.initials: H
authors.lastname: Fares
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism*; D002451:Cell Compartmentation; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D004705:Endocytosis / Q000235:genetics; D017238:Genes, Helminth; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D049452:Green Fluorescent Proteins
keywords: Caenorhabditis elegans; coelomocyte; lysosome