title: LPS Cooperates with Poly-L-Arginine to Promote IL-6 and IL-8 Release via the JNK Signaling Pathway in NCI-H292 Cells.
abstract: Objective. Herein, we aimed to study the mechanism whereby poly-L-arginine (PLA) and lipopolysaccharide (LPS) can synergistically induce the release of interleukin-6 (IL-6) and IL-8 in NCI-H292 cells.Methods. NCI-H292 cells were divided into control, PLA, LPS, and PLA+LPS groups. At various time points, the phosphorylation of JNK in each group was measured by western blotting. Additionally, the productions of IL-6 and IL-8 were assessed using an enzyme-linked immunosorbent assay (ELISA). The effects of SP600125, an inhibitor of the JNK pathway, on the increase of p-JNK, IL-6, and IL-8 were also studied.Results. Our results showed that either PLA or LPS treatment alone can significantly increase the phosphorylation level of JNK in NCI-H292 cells. Of interest was the combined use of PLA and LPS that has a synergistic effect on the phosphorylation of JNK, as well as synergistically inducing the release of IL-6 and IL-8 in NCI-H292 cells. Furthermore, SP600125 significantly inhibited the activation of JNK signal, as well as reducing the productions of IL-6 and IL-8 in response to PLA+LPS stimulation.Conclusions. The JNK signaling pathway contributes to the release of IL-6 and IL-8, which is stimulated by the synergistic actions of PLA+LPS in NCI-H292 cells.
authors.affiliation: Department of Geriatric Respiratory Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China.
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Zhang
authors.affiliation: Department of Geriatric Respiratory Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.affiliation: Department of Geriatric Respiratory Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China.
authors.forename: Xiao-Yun
authors.identifier: 0000-0002-6556-3694
authors.initials: XY
authors.lastname: Fan
authors.affiliation: Department of Geriatric Respiratory Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China.
authors.forename: Sha-Sha
authors.initials: SS
authors.lastname: Wu
authors.affiliation: Department of Biochemistry and Molecular Biology, Anhui Medical University, Number 81, Meishan Road, Hefei, Anhui 230022, China.
authors.forename: Sheng-Quan
authors.initials: SQ
authors.lastname: Zhang
authors.affiliation: Department of Geriatric Respiratory Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China.
authors.forename: Hui-Mei
authors.initials: HM
authors.lastname: Wu
mesh_terms: D000873:Anthracenes / Q000494:pharmacology; D001249:Asthma / Q000473:pathology; D045744:Cell Line, Tumor; D004357:Drug Synergism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis / Q000378:metabolism*; D016209:Interleukin-8 / Q000096:biosynthesis / Q000378:metabolism*; D048031:JNK Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D010455:Peptides / Q000494:pharmacology*; D010766:Phosphorylation / Q000187:drug effects
chemical_list: D000873:Anthracenes; C508606:CXCL8 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D016209:Interleukin-8; D008070:Lipopolysaccharides; D010455:Peptides; C432165:pyrazolanthrone; C015462:polyarginine; D048031:JNK Mitogen-Activated Protein Kinases