title: DNA Detection by Flow Cytometry using PNA-Modified Metal-Organic Framework Particles.
abstract: A DNA-sensing platform is developed by exploiting the easy surface functionalization of metal-organic framework (MOF) particles and their highly parallelized fluorescence detection by flow cytometry. Two strategies were employed to functionalize the surface of MIL-88A, using either covalent or non-covalent interactions, resulting in alkyne-modified and biotin-modified MIL-88A, respectively. Covalent surface coupling of an azide-dye and the alkyne-MIL-88A was achieved by means of a click reaction. Non-covalent streptavidin-biotin interactions were employed to link biotin-PNA to biotin-MIL-88A particles mediated by streptavidin. Characterization by confocal imaging and flow cytometry demonstrated that DNA can be bound selectively to the MOF surface. Flow cytometry provided quantitative data of the interaction with DNA. Making use of the large numbers of particles that can be simultaneously processed by flow cytometry, this MOF platform was able to discriminate between fully complementary, single-base mismatched, and randomized DNA targets.
authors.affiliation: Molecular NanoFabrication group, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500, AE Enschede, The Netherlands.
authors.forename: Raquel
authors.initials: R
authors.lastname: Mejia-Ariza
authors.affiliation: Molecular NanoFabrication group, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500, AE Enschede, The Netherlands.|Department of Chemistry, University of Parma, Parma, Italy.
authors.forename: Jessica
authors.initials: J
authors.lastname: Rosselli
authors.affiliation: Medical Cell BioPhysics, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, P.O. Box 217, 7500, AE Enschede, The Netherlands.
authors.forename: Christian
authors.initials: C
authors.lastname: Breukers
authors.affiliation: Department of Chemistry, University of Parma, Parma, Italy.
authors.forename: Alex
authors.initials: A
authors.lastname: Manicardi
authors.affiliation: Medical Cell BioPhysics, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, P.O. Box 217, 7500, AE Enschede, The Netherlands.
authors.forename: Leon W M M
authors.initials: LW
authors.lastname: Terstappen
authors.affiliation: Department of Chemistry, University of Parma, Parma, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Corradini
authors.affiliation: Molecular NanoFabrication group, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500, AE Enschede, The Netherlands.
authors.forename: Jurriaan
authors.identifier: http://orcid.org/0000-0002-4596-9179
authors.initials: J
authors.lastname: Huskens
mesh_terms: D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D057930:Click Chemistry; D061565:Cycloaddition Reaction; D004247:DNA / Q000032:analysis*; D005290:Ferric Compounds / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D000073396:Metal-Organic Frameworks / Q000737:chemistry*; D010316:Particle Size; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000480:Alkynes; D001386:Azides; D005290:Ferric Compounds; D005456:Fluorescent Dyes; D000073396:Metal-Organic Frameworks; D020135:Peptide Nucleic Acids; C000499:ferric oxide; D011092:Polyethylene Glycols; D001710:Biotin; D004247:DNA; D019809:Streptavidin
keywords: metal-organic frameworks; multivalent biomaterials; nanoparticles; nucleic acid recognition; peptide nucleic acids