title: T-6ballocates more assimilation product for oil synthesis and less for polysaccharide synthesis during the seed development ofArabidopsis thaliana.
abstract: BACKGROUND
  As anAgrobacterium tumefaciensT-DNA oncogene, T-6binduces the development of tumors and the enation syndrome in vegetative tissues of transgenic plants. Most of these effects are related to increases in soluble sugar contents. To verify the potential roles of T-6b in the distribution of carbon in developing seeds, not in vegetative tissues, we fused an endosperm-specific promoter to the T-6bgene for expression in transgenicArabidopsis thalianaplants.
  
  
  RESULTS
  The expression of T-6bin reproductive organs did not induce the development of the enation syndrome, and moreover, promoted endosperm expansion, which increased the total seed biomass by more than 10%. Additionally, T-6b also increased oil content in mature seeds by more than 10% accompanied with the decrease of starch and mucilage content at the same time.
  
  
  CONCLUSIONS
  T-6b enhances seed biomass and helps oil biosynthesis but not polysaccharides in reproductive organs without disturbing vegetative growth and development. Our findings suggest T-6bmay be very useful for increasing oil production in biodiesel plants.
authors.affiliation: Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha, 410128 China.|Department of Plant Biology, Uppsala BioCenter, Linnean Center for Plant Biology, Swedish University of Agricultural Sciences, PO Box 7080, SE-75007 Uppsala, Sweden.
authors.forename: Yunkai
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha, 410128 China.|Key Laboratory of Education, Department of Hunan Province on Plant Genetics and Molecular Biology, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128 China.
authors.forename: Jia
authors.initials: J
authors.lastname: Hu
authors.affiliation: Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha, 410128 China.|Key Laboratory of Education, Department of Hunan Province on Plant Genetics and Molecular Biology, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128 China.
authors.forename: Xun
authors.initials: X
authors.lastname: Liu
authors.affiliation: Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha, 410128 China.|Key Laboratory of Education, Department of Hunan Province on Plant Genetics and Molecular Biology, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128 China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ruan
authors.affiliation: Department of Plant Biology, Uppsala BioCenter, Linnean Center for Plant Biology, Swedish University of Agricultural Sciences, PO Box 7080, SE-75007 Uppsala, Sweden.
authors.forename: Chuanxin
authors.initials: C
authors.lastname: Sun
authors.affiliation: Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha, 410128 China.
authors.forename: Chunlin
authors.initials: C
authors.lastname: Liu
keywords: Biosynthesis; Endosperm expression; Lipid; Mucilage; Starch; T-6b