title: Replication of neurovirulent equine herpesvirus type 1 (EHV-1) in CD172a+monocytic cells.
abstract: Equine herpesvirus type 1 (EHV-1) is responsible for respiratory disorders, abortion and myeloencephalopathy (EHM) in horses. Two pathotypes of EHV-1 strains are circulating in the field: neurovirulent (N) and non-neurovirulent (NN). For both strains, CD172a+monocytic cells are one of the main carrier cells of EHV-1 during primary infection, allowing the virus to invade the horse's body. Recently, we showed that EHV-1 NN strains showed a restricted and delayed replication in CD172a+cells. Here we characterize the in vitro replication kinetics of two EHV-1N strains in CD172a+cells and investigate if the replication of these strains is similarly silenced as shown for EHV-1 NN strains. We found that EHV-1N replication was restricted to 7-8% in CD172a+cells compared to 100% in control RK-13 cells. EHV-1N replication was not delayed in CD172a+cells but virus production was significant lower (103.0TCID50/105inoculated cells) than in RK-13 cells (108.5TCID50/105inoculated cells). Approximately 0.04% of CD172a+cells produced and transmitted infectious EHV-1 to neighbour cells compared to 65% of RK-13 cells. Unlike what we observed for the NN strain, pretreatment of CD172a+cells with histone deacetylases inhibitors (HDACi) did not influence the replication of EHV-1N strains in these cells. Overall, these results show that the EHV-1 replication of N strains in CD172a+cells differs from that observed for NN strains, which may contribute to their different pathogeneses in vivo.
authors.affiliation: Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
authors.forename: Kathlyn
authors.initials: K
authors.lastname: Laval
authors.affiliation: Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
authors.forename: Jolien
authors.initials: J
authors.lastname: Van Cleemput
authors.affiliation: Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
authors.forename: Katrien C
authors.initials: KC
authors.lastname: Poelaert
authors.affiliation: Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
authors.forename: Ivy K
authors.initials: IK
authors.lastname: Brown
authors.affiliation: Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. Electronic address: hans.nauwynck@ugent.be.
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Nauwynck
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004861:Herpesvirus 1, Equid / Q000472:pathogenicity / Q000502:physiology*; D006736:Horses; D009000:Monocytes / Q000821:virology*; D014779:Virus Replication
keywords: CD172a monocytic cells; EHV-1,replication; Neurovirulent