title: Genistein Suppression of Matrix Metalloproteinase 2 (MMP-2) and Vascular Endothelial Growth Factor (VEGF) Expression in Mesenchymal Stem Cell Like Cells Isolated from High and Low Grade Gliomas
abstract: Objective: Brain tumors cause great mortality and morbidity worldwide, and success rates with surgical
  treatment remain very low. Several recent studies have focused on introduction of novel effective medical therapeutic
  approaches. Genistein is a member of the isoflavonoid family which has proved to exert anticancer effects. Here we
  assessed the effects of genistein on the expression of MMP-2 and VEGF in low and high grade gliomas in vitro. Materials
  and Methods: High and low grade glioma tumor tissue samples were obtained from a total of 16 patients, washed
  with PBS, cut into small pieces, digested with collagenase type I and cultured in DMEM containing 10% FBS. When
  cells reached passage 3, they were exposed to genistein and MMP-2 and VEGF gene transcripts were determined by
  quantitative real time PCR (qRT-PCR). Results: Expression of MMP-2 demonstrated 580-fold reduction in expression
  in low grade glioma cells post treatment with genistein compared to untreated cells (P value= 0.05). In cells derived
  from high grade lesions, expression of MMP-2 was 2-fold lower than in controls (P value> 0.05). Genistein caused a
  4.7-fold reduction in VEGF transcript in high grade glioma cells (P value> 0.05) but no effects were evident in low
  grade glioma cells. Conclusion. Based on the data of the present study, low grade glioma cells appear much more
  sensitive to genistein and this isoflavone might offer an appropriate therapeutic intervention in these patients. Further
  investigation of this possibility is clearly warranted.
authors.affiliation: Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Email: razmkhahm@sums.ac.ir
authors.forename: Yasaman
authors.initials: Y
authors.lastname: Yazdani
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Sharifi Rad
authors.forename: Mousa
authors.initials: M
authors.lastname: Taghipour
authors.forename: Nooshafarin
authors.initials: N
authors.lastname: Chenari
authors.forename: Abbas
authors.initials: A
authors.lastname: Ghaderi
authors.forename: Mahboobeh
authors.initials: M
authors.lastname: Razmkhah
keywords: Glioma; genistein; matrix metalloproteinase 2; vascular endothelial growth factor