title: Culture adaptation of malaria parasites selects for convergent loss-of-function mutants.
abstract: Cultured human pathogens may differ significantly from source populations. To investigate the genetic basis of laboratory adaptation in malaria parasites, clinical Plasmodium falciparum isolates were sampled from patients and cultured in vitro for up to three months. Genome sequence analysis was performed on multiple culture time point samples from six monoclonal isolates, and single nucleotide polymorphism (SNP) variants emerging over time were detected. Out of a total of five positively selected SNPs, four represented nonsense mutations resulting in stop codons, three of these in a single ApiAP2 transcription factor gene, and one in SRPK1. To survey further for nonsense mutants associated with culture, genome sequences of eleven long-term laboratory-adapted parasite strains were examined, revealing four independently acquired nonsense mutations in two other ApiAP2 genes, and five in Epac. No mutants of these genes exist in a large database of parasite sequences from uncultured clinical samples. This implicates putative master regulator genes in which multiple independent stop codon mutations have convergently led to culture adaptation, affecting most laboratory lines of P. falciparum. Understanding the adaptive processes should guide development of experimental models, which could include targeted gene disruption to adapt fastidious malaria parasite species to culture.
authors.affiliation: London School of Hygiene and Tropical Medicine, London, UK.|Medical Research Council Unit The Gambia, Atlantic Road, Fajara, P.O. Box 273, Banjul, The Gambia.
authors.forename: Antoine
authors.initials: A
authors.lastname: Claessens
authors.affiliation: Medical Research Council Unit The Gambia, Atlantic Road, Fajara, P.O. Box 273, Banjul, The Gambia.
authors.forename: Muna
authors.initials: M
authors.lastname: Affara
authors.affiliation: London School of Hygiene and Tropical Medicine, London, UK.
authors.forename: Samuel A
authors.initials: SA
authors.lastname: Assefa
authors.affiliation: The Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom.
authors.forename: Dominic P
authors.initials: DP
authors.lastname: Kwiatkowski
authors.affiliation: London School of Hygiene and Tropical Medicine, London, UK.
authors.forename: David J
authors.initials: DJ
authors.lastname: Conway
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D000483:Alleles; D000818:Animals; D018389:Codon, Nonsense / Q000235:genetics; D016678:Genome; D006801:Humans; D000073658:Loss of Function Mutation / Q000235:genetics*; D016778:Malaria, Falciparum / Q000469:parasitology*; D010271:Parasites / Q000302:isolation & purification / Q000502:physiology*; D010963:Plasmodium falciparum / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D018389:Codon, Nonsense