title: Aptamer-molecularly imprinted sensor base on electrogenerated chemiluminescence energy transfer for detection of lincomycin.
abstract: In this study, a biosensor with a dual recognition system comprising a molecularly imprinted polymer (MIP) and aptamers selective for lincomycin was fabricated. The MIP was synthesized by electropolymerization of carbon dots (C-dots)-tagged DNA aptamers combined with lincomycin and o-aminophenol on the gold-nanoparticle-functionalized graphene oxide (Au-GO)-modified electrode. Electrogenerated chemiluminescence (ECL) resonance energy transfer was observed between Au-GO and C-dots. After the C-dots accepted the energy, they acted as a signal indicator and exhibited enhanced signal intensity in the presence of target lincomycin. When lincomycin was competitively bound to DNA aptamers and MIP, it blocked the transfer of energy, and a decreased ECL signal was observed. Hence, a dual recognition method for the detection of lincomycin is realized. Using this strategy, the sensor exhibited a linear ECL response to lincomycin at concentrations from 5.0 × 10-12mol/L to 1.0 × 10-9mol/L. The detection limit of this assay was found to be 1.6 × 10-13mol/L. This method was utilized to determine lincomycin residuals in meat samples with satisfactory results.
authors.affiliation: Analysis and Test Center of Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China; Laboratory of Quality & Safety Risk Assessment for Tropical Products (Haikou) Ministry of Agriculture, Haikou, 571101, China. Electronic address: happylishuhuai@163.com.
authors.forename: Shuhuai
authors.initials: S
authors.lastname: Li
authors.affiliation: Analysis and Test Center of Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China; Laboratory of Quality & Safety Risk Assessment for Tropical Products (Haikou) Ministry of Agriculture, Haikou, 571101, China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Liu
authors.affiliation: Analysis and Test Center of Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China; Laboratory of Quality & Safety Risk Assessment for Tropical Products (Haikou) Ministry of Agriculture, Haikou, 571101, China.
authors.forename: Guihao
authors.initials: G
authors.lastname: Yin
authors.affiliation: Analysis and Test Center of Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China; Laboratory of Quality & Safety Risk Assessment for Tropical Products (Haikou) Ministry of Agriculture, Haikou, 571101, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Analysis and Test Center of Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China; Laboratory of Quality & Safety Risk Assessment for Tropical Products (Haikou) Ministry of Agriculture, Haikou, 571101, China. Electronic address: luocatas@21cn.com.
authors.forename: Jinhui
authors.initials: J
authors.lastname: Luo
authors.affiliation: Analysis and Test Center of Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China; Laboratory of Quality & Safety Risk Assessment for Tropical Products (Haikou) Ministry of Agriculture, Haikou, 571101, China.
authors.forename: Nanchun
authors.initials: N
authors.lastname: Wu
mesh_terms: D000627:Aminophenols / Q000737:chemistry; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D004735:Energy Transfer; D005506:Food Contamination / Q000032:analysis*; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D057230:Limit of Detection; D008034:Lincomycin / Q000032:analysis*; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods; D008460:Meat / Q000032:analysis*; D054802:Molecular Imprinting / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry; D058105:Polymerization
chemical_list: D000627:Aminophenols; D000900:Anti-Bacterial Agents; D052157:Aptamers, Nucleotide; C027667:2-aminophenol; D006046:Gold; D006108:Graphite; D008034:Lincomycin
keywords: Aptamer; Dual recognition; Energy transfer; Molecularly imprinted; lincomycin