title: Alteration of thiol-disulfide homeostasis in workers occupationally exposed to arsenic.
abstract: This study evaluates the redox state in occupationally arsenic-exposed workers (n = 71) by assessing the dynamic serum thiol-disulfide homeostasis. We determined the serum thiol-disulfide homeostasis parameters of exposed workers and controls (n = 43) using a novel automated colorimetric assay. Median urinary As and 8-isoprostane levels of exposed group were significantly higher than control group (16.40 μg/L vs 2μg/L, p < .001 and 2.28 ng/ml vs 0.54 ng/ml, p < .001, respectively). Disulfide level, disulfide/native thiol ratio, and disulfide/total thiol ratio were significantly higher in exposed group. The mean ceruloplasmine and myeloperoxidase activities of As-exposed group were significantly higher than control group (117.15 U/L vs 87.02 U/L, p = .035 and 148.53 U/L vs 97.75 U/L, p = .000, respectively). The median catalase activity did not differ in the two groups. Our findings revealed that As disrupts the thiol-disulfide homeostasis in favor of disulfide.
authors.affiliation: a Department of Pharmacology , Occupational Diseases Hospital , Ankara , Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Büyükşekerci
authors.affiliation: b Department of Biochemistry , Yıldırım Beyazıt University , Ankara , Turkey.
authors.forename: Ceylan
authors.initials: C
authors.lastname: Bal
authors.affiliation: c Department of Biochemistry , Gölbaşı Hasvak State Hospital , Ankara , Turkey.
authors.forename: Utku
authors.initials: U
authors.lastname: Serkant
authors.affiliation: d Department of Family Medicine , Occupational Diseases Hospital , Ankara , Turkey.
authors.forename: Meşide
authors.initials: M
authors.lastname: Gündüzöz
authors.affiliation: b Department of Biochemistry , Yıldırım Beyazıt University , Ankara , Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Alışık
authors.affiliation: e Department of Public Health , Bozok University , Yozgat , Turkey.
authors.forename: Engin
authors.initials: E
authors.lastname: Tutkun
authors.affiliation: f Department of Public Health , Yıldırım Beyazıt University , Ankara , Turkey.
authors.forename: Ömer Hınç
authors.initials: ÖH
authors.lastname: Yılmaz
mesh_terms: D000328:Adult; D001151:Arsenic / Q000652:urine*; D004220:Disulfides / Q000097:blood*; D006706:Homeostasis; D006801:Humans; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure; D013438:Sulfhydryl Compounds / Q000097:blood*
chemical_list: D004220:Disulfides; D013438:Sulfhydryl Compounds; D001151:Arsenic
keywords: Arsenic exposure; isoprostane; oxidative stress; thiol-disulfide homeostasis