title: Development of a method for efficient cost-effective screening of Aspergillus niger mutants having increased production of glucoamylase.
abstract: OBJECTIVE
  To develop an efficient cost-effective screening process to improve production of glucoamylase in Aspergillus niger.
  
  
  RESULTS
  The cultivation of A. niger was achieved with well-dispersed morphology in 48-deep-well microtiter plates, which increased the throughput of the samples compared to traditional flask cultivation. There was a close negative correlation between glucoamylase and its pH of the fermentation broth. A novel high-throughput analysis method using Methyl Orange was developed. When compared to the conventional analysis method using 4-nitrophenyl α-D-glucopyranoside as substrate, a correlation coefficient of 0.96 by statistical analysis was obtained.
  
  
  CONCLUSIONS
  Using this novel screening method, we acquired a strain with an activity of 2.2 × 103U ml-1, a 70% higher yield of glucoamylase than its parent strain.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
authors.forename: Bessembayev
authors.initials: B
authors.lastname: Arman
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, People's Republic of China. juchu@ecust.edu.cn.
authors.forename: Ju
authors.initials: J
authors.lastname: Chu
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
authors.forename: Yingping
authors.initials: Y
authors.lastname: Zhuang
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D019149:Bioreactors; D005087:Glucan 1,4-alpha-Glucosidase / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D057166:High-Throughput Screening Assays / Q000191:economics / Q000295:instrumentation / Q000379:methods*
chemical_list: D005087:Glucan 1,4-alpha-Glucosidase
keywords: Aspergillus niger; Glucoamylase; High-throughput screening; Microtiter plates