title: Injection of SDF-1 loaded nanoparticles following traumatic brain injury stimulates neural stem cell recruitment.
abstract: Recruiting neural stem cell (NSC) at the lesion site is essential for central nervous system repair. This process could be triggered by the local delivery of the chemokine SDF-1. We compared two PLGA formulations for local brain SDF-1 delivery: SDF-1 loaded microspheres (MS) and SDF-1 loaded nanoparticles (NP). Both formulations were able to encapsulate more than 80% of SDF-1 but presented different release profiles, with 100% of SDF-1 released after 6days for the MS and with 25% of SDF-1 released after 2 weeks for NP. SDF-1 bioactivity was demonstrated by a chemotactic assay. When injected in mouse brain after traumatic brain injury, only SDF-1 nanoparticles induced NSC migration to the damage area. More neuroblasts (DCX+ cells) could be visualized around the lesions treated with NP SDF-1 compared to the other conditions. Rostral migratory stream destabilization with massive migration of DCX+ cell toward the perilesional area was observed 2 weeks after NP SDF-1 injection. Local injection of SDF-1-loaded nanoparticles induces recruitment of NSC and could be promising for brain injury lesion.
authors.affiliation: Neurobiology Lab, Research Building II, Universidade Federal de São Paulo, Rua Pedro de Toledo 669, Vila Clementino, São Paulo, CEP: 04039-032, Brazil.
authors.forename: Laura N
authors.initials: LN
authors.lastname: Zamproni
authors.affiliation: Neurobiology Lab, Research Building II, Universidade Federal de São Paulo, Rua Pedro de Toledo 669, Vila Clementino, São Paulo, CEP: 04039-032, Brazil.
authors.forename: Mayara V
authors.initials: MV
authors.lastname: Mundim
authors.affiliation: Neurobiology Lab, Research Building II, Universidade Federal de São Paulo, Rua Pedro de Toledo 669, Vila Clementino, São Paulo, CEP: 04039-032, Brazil.
authors.forename: Marimelia A
authors.initials: MA
authors.lastname: Porcionatto
authors.affiliation: Université catholique de Louvain, Louvain Drug Research Institute, Advanced Drug Delivery and Biomaterials, Avenue E. Mounier 73, 1200 Bruxelles, Belgium; Université catholique de Louvain, Institute of Materials and Condensed Matter, 1348 Louvain-la-Neuve, Belgium. Electronic address: anne.desrieux@uclouvain.be.
authors.forename: Anne
authors.initials: A
authors.lastname: des Rieux
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects; D000070642:Brain Injuries, Traumatic / Q000188:drug therapy*; D002465:Cell Movement / Q000187:drug effects; D000697:Central Nervous System Stimulants / Q000008:administration & dosage*; D002626:Chemistry, Pharmaceutical / Q000379:methods; D054377:Chemokine CXCL12 / Q000008:administration & dosage*; D000089662:Doublecortin Protein; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008863:Microspheres; D053758:Nanoparticles / Q000008:administration & dosage*; D058953:Neural Stem Cells / Q000187:drug effects*
chemical_list: D000697:Central Nervous System Stimulants; D054377:Chemokine CXCL12; C000714847:Dcx protein, mouse; D000089662:Doublecortin Protein
keywords: Brain injury; Nanoparticles; Neural stem cells; SDF-1