title: Genetic characterization of two novel megriviruses in geese.
abstract: Two goose megriviruses (W18 and HN56) were detected and sequenced. Both viruses possessed megrivirus-like genomic features, including unusually long genomes (9840 and 10 101 nt). W18 and HN56 were very similar to duck megrivirus (DMV) in the P2 and P3 regions, but much less similar in the P1 and 2A1 regions. HN56 may be a potential recombinant virus, with a distinct P1 region possibly originating from an unknown picornavirus. W18 may represent a novel type of DMV, showing a P1 sequence identity of 67 %. Similar levels (64-68 %) of P1 sequence identity were also displayed by melegrivirus A with W18 and DMV, demonstrating an equal genetic separation of the capsid region among W18, DMV and melegrivirus A. For the 2A1 region, the divergence among W18, HN56 and DMV was remarkable, involving point mutations and a long insertion/deletion. The present work contributes to the understanding of unique features and phylogeny of megriviruses.
authors.affiliation: Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, PR China.
authors.forename: Fumin
authors.initials: F
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, PR China.
authors.forename: Te
authors.initials: T
authors.lastname: Liang
authors.affiliation: Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.affiliation: Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, PR China.
authors.forename: Kang
authors.initials: K
authors.lastname: Ning
authors.affiliation: The Ministry of Agriculture of the People's Republic of China, Beijing 100026, PR China.
authors.forename: Kangzhen
authors.initials: K
authors.lastname: Yu
authors.affiliation: Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, PR China.
authors.forename: Dabing
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D005777:Geese / Q000821:virology*; D023061:Gene Order; D014644:Genetic Variation; D010802:Phylogeny; D010849:Picornaviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D026801:Synteny