title: Renoprotective chemical constituents from an edible mushroom, Pleurotus cornucopiae in cisplatin-induced nephrotoxicity.
abstract: Pleurotus cornucopiae (Pleurotaceae) is an edible and medicinal mushroom widely distributed in Korea, China, and Japan. The MeOH extract of the fruiting bodies of P. cornucopiae showed renoprotective effects against cisplatin-induced kidney cell damage. Chemical investigation of the MeOH extract led to the isolation and identification of 12 compounds including noransine (1), uridine (2), uracil (3), (3β, 5α, 6β, 22E, 24S) -ergosta-7, 22-diene-3, 5, 6, 9-tetrol (4), (22E,24S)-ergosta-7,22-diene-3β,5α,6β-triol (5), (22E,24R)-ergosta-8(14),22-diene-3β,5α,6β,7α-tetrol (6), cerebroside B (7), (2R) -N- [(1S, 2R, 3E, 7E) -1- [(β-d-glucopyranosyloxy) methyl] -2-hydroxy-8-methyl-3, 7-heptadecadien-1-yl] -2-hydroxy-heptadecanamide (8), cerebroside D (9), nicotinamide (10), 1,2-bis(hydroxymethyl)-4,5-dimethoxybenzene (11), and benzoic acid (12). Among them, compounds 1 and 11 were isolated as naturally occurring products for the first time, though they were reported as synthetic products in previous papers. All of the compounds (except 8 and 11) abrogated cisplatin-induced LLC-PK1 cell damage in a dose-dependent manner. Of special note, compounds 2, 5, 6, and 12 ameliorated cisplatin-induced nephrotoxicity to 80% of the control value at 10μM. The protective effects of compounds 2, 5, 6, and 12 were mediated via the deactivation of JNK-caspase 3 apoptotic cascade. This study is the first to demonstrate that the chemical constituents of P. cornucopiae display renoprotective effects against anticancer drug-induced damage in kidney cells.
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
authors.forename: Seoung Rak
authors.initials: SR
authors.lastname: Lee
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
authors.forename: Dahae
authors.initials: D
authors.lastname: Lee
authors.affiliation: Department of Food and Nutrition, Gachon University, Seongnam 13120, Republic of Korea.
authors.forename: Hae-Jeung
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA, Eumseoung 369-873, Republic of Korea.
authors.forename: Hyung Jun
authors.initials: HJ
authors.lastname: Noh
authors.affiliation: College of Pharmacy, CHA University, Pocheon 487-010, Republic of Korea.
authors.forename: Kiwon
authors.initials: K
authors.lastname: Jung
authors.affiliation: College of Korean Medicine, Gachon University, Seongnam 13120, Republic of Korea.
authors.forename: Ki Sung
authors.initials: KS
authors.lastname: Kang
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Republic of Korea. Electronic address: khkim83@skku.edu.
authors.forename: Ki Hyun
authors.initials: KH
authors.lastname: Kim
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000009:adverse effects*; D017209:Apoptosis / Q000187:drug effects; D001688:Biological Products / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D002945:Cisplatin / Q000009:adverse effects*; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D020076:Pleurotus / Q000737:chemistry*; D020011:Protective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013552:Swine
chemical_list: D000970:Antineoplastic Agents; D001688:Biological Products; D020011:Protective Agents; D002945:Cisplatin
keywords: MAPKs; Mushroom; Nephrotoxicity; Pleurotaceae; Pleurotus cornucopiae