title: Analysis of pancreatic disease in zebrafish.
abstract: One of the appeals of the zebrafish model is the relative ease of studying disease progression from embryonic or larval stages through to adulthood. Because of this, the zebrafish has become an important model for postembryonic pancreatic disease, particularly diabetes and pancreatic cancer. Here we present methods for using the adult zebrafish to analyze pancreas function and structure, with an emphasis on the endocrine pancreas and the beta cells. The methods include fasting, weighing adults, and anesthetizing adults, and intraperitoneal injection of glucose based on body weight. We also present dissection methods for removing the pancreas intact for histological studies and for sterile dissection of the principal islet followed by dissociation for cell culture-based studies of beta-cell function.
authors.affiliation: Genentech, Inc., South San Francisco, CA, United States.
authors.forename: S C
authors.initials: SC
authors.lastname: Eames Nalle
authors.affiliation: Appalachian State University, Boone, NC, United States.
authors.forename: K F
authors.initials: KF
authors.lastname: Franse
authors.affiliation: Appalachian State University, Boone, NC, United States.
authors.forename: M D
authors.initials: MD
authors.lastname: Kinkel
mesh_terms: D000818:Animals; D003920:Diabetes Mellitus / Q000000981:diagnostic imaging / Q000503:physiopathology; D004195:Disease Models, Animal; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D050417:Insulin-Secreting Cells / Q000473:pathology / Q000648:ultrastructure*; D057054:Molecular Imaging / Q000379:methods*; D010179:Pancreas / Q000503:physiopathology / Q000648:ultrastructure*; D010182:Pancreatic Diseases / Q000000981:diagnostic imaging* / Q000503:physiopathology; D010190:Pancreatic Neoplasms / Q000000981:diagnostic imaging / Q000503:physiopathology; D015027:Zebrafish / Q000235:genetics
keywords: Blood glucose; Cell culture; Cold-water anesthesia; Diabetes; Dissection; Endocrine pancreas; Fasting; IP injection; Islet isolation; Weighing