title: Icariin combined with human umbilical cord mesenchymal stem cells significantly improve the impaired kidney function in chronic renal failure.
abstract: At present, the main therapy for chronic renal failure (CRF) is dialysis and renal transplantation, but neither obtains satisfactory results. Human umbilical cord mesenchymal stem cells (huMSCs) are isolated from the fetal umbilical cord which has a high self-renewal and multi-directional differentiation potential. Icariin (ICA), a kidney-tonifying Chinese Medicine can enhance the multipotency of huMSCs. Therefore, this work seeks to employ the use of ICA-treated huMSCs for the treatment of chronic renal failure. Blood urea nitrogen and creatinine (Cr) analyses showed amelioration of functional parameters in ICA-treated huMSCs for the treatment of CRF rats at 3, 7, and 14 days after transplantation. ICA-treated huMSCs can obviously increase the number of cells in injured renal tissues at 3, 7, and 14 days after transplantation by optical molecular imaging system. Hematoxylin-eosin staining demonstrated that ICA-treated huMSCs reduced the levels of fibrosis in CRF rats at 14 days after transplantation. Superoxide dismutase and Malondialdehyde analyses showed that ICA-treated huMSCs reduced the oxidative damage in CRF rats. Moreover, transplantation with ICA-treated huMSCs decreased inflammatory responses, promoted the expression of growth factors, and protected injured renal tissues. Taken together, our findings suggest that ICA-treated huMSCs could improve the kidney function in CRF rats.
authors.affiliation: School of Medicine, State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Nankai University, Tianjin, 300071, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.affiliation: Tianjin Second People's Hospital, Tianjin, 300192, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Tianjin People's Hospital, Tianjin, 300000, China.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Chu
authors.affiliation: Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China.
authors.forename: Huantian
authors.initials: H
authors.lastname: Cui
authors.affiliation: Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China. bianyuhong_2012@163.com.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Bian
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005419:Flavonoids / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D007676:Kidney Failure, Chronic / Q000378:metabolism / Q000503:physiopathology* / Q000628:therapy*; D007677:Kidney Function Tests; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D016896:Treatment Outcome; D014470:Umbilical Cord / Q000166:cytology* / Q000187:drug effects
chemical_list: D005419:Flavonoids; D036341:Intercellular Signaling Peptides and Proteins; C056599:icariin
keywords: Chronic renal failure; Human umbilical cord mesenchymal stem cells; Icariin; Kidney function