title: Tang-Luo-Ning Improves Mitochondrial Antioxidase Activity in Dorsal Root Ganglia of Diabetic Rats: A Proteomics Study.
abstract: Tang-luo-ning (TLN) is a traditional Chinese herbal recipe for treating diabetic peripheral neuropathy (DPN). In this study, we investigated mitochondrial protein profiles in a diabetic rat model and explored the potential protective effect of TLN. Diabetic rats were established by injection of streptozocin (STZ) and divided into model, alpha lipoic acid (ALA), and TLN groups. Mitochondrial proteins were isolated from dorsal root ganglia and proteomic analysis was used to quantify the differentially expressed proteins. Tang-luo-ning mitigated STZ-induced diabetic symptoms and blood glucose level, including response time to cold or hot stimulation and nerve conductive velocity. As compared to the normal, there were 388 differentially expressed proteins in the TLN group, 445 in ALA group, and 451 in model group. As compared to the model group, there were 275 differential proteins in TLN group and 251 in ALA group. As compared to model group, mitochondrial complex III was significantly decreased, while glutathione peroxidase and peroxidase were increased in TLN group. When compared with ALA group, the mitochondrial complex III was increased, and mitochondrial complex IV was decreased in TLN group. Together, TLN should have a strong antioxidative activity, which appears to be modulated through regulation of respiratory complexes and antioxidases.
authors.affiliation: Department of Endocrinology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China.
authors.forename: Taojing
authors.identifier: 0000-0001-8839-6406
authors.initials: T
authors.lastname: Zhang
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Endocrinology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China.
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Gong
authors.affiliation: Department of Endocrinology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Endocrinology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China.
authors.forename: Peifeng
authors.initials: P
authors.lastname: Xie
authors.affiliation: Department of Endocrinology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China.
authors.forename: Song
authors.initials: S
authors.lastname: Guan
authors.affiliation: Department of Endocrinology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China.
authors.forename: Wenming
authors.initials: W
authors.lastname: Yi
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy* / Q000503:physiopathology; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D005727:Ganglia, Spinal / Q000187:drug effects / Q000201:enzymology*; D006442:Glycated Hemoglobin / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology*; D024101:Mitochondrial Proteins / Q000378:metabolism; D009431:Neural Conduction / Q000187:drug effects; D010088:Oxidoreductases / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D017207:Rats, Sprague-Dawley; D013311:Streptozocin; D008063:Thioctic Acid / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D001786:Blood Glucose; D004365:Drugs, Chinese Herbal; D006442:Glycated Hemoglobin; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D010455:Peptides; D013311:Streptozocin; D008063:Thioctic Acid; C000599174:tangningtongluo