title: Identification of Group B Streptococci Using 16S rRNA, cfb, scpB, and atr Genes in Pregnant Women by PCR.
abstract: Streptococcus agalactiae is acommensalorganism, but it may cause infection in susceptible hosts. The aim of this study was to evaluate PCR assay compared with conventional culture method for direct detection of Streptococcus agalactiae. Total of 203 paired low vaginal swabs were collected from women at 35-37 weeks of pregnancy from June 2013 through February 2014 for detection of Streptococcus agalactiae using PCR assay targeting 16S rRNA, cfb, scpB, and atr genes and culture method following broth enrichment. The results were recorded and evaluated for determining of sensitivity, specificity, positive and negative predictive values of PCR assaycompared with culture method. Prevalence of Streptococcus agalactiae was determined as 7.39% (n=15) using culture method; 19.70% (n=40) by PCR targeting 16S rRNA gene; 18.23% (n=37) by targeting atr gene; 17.24% (n=35) by cfb gene; and 8.87% (n=18) by scpB gene. Generally, a total of 49 specimens were considered true positive (27 samples by PCR assay using the four genes in sum, 4 samples only by atr gene PCR, 3 samples only by cfb gene PCR, 2 samples only by culture method, and 13 samples by PCR assay and culture method in common) and prevalence of Streptococcus agalactiae determined 24.14% in Hamadan. The current data demonstrated that performing only culture method for detecting GBS from pregnant women leads to missed false negative carrier individuals. Thus, it is recommended that both the PCR assay and conventional culture method to be performed in order to detect Streptococcus agalactiae.
authors.affiliation: Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Seyed Masoud
authors.initials: SM
authors.lastname: Mousavi
authors.affiliation: Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Seyed Mostafa
authors.initials: SM
authors.lastname: Hosseini
authors.affiliation: Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Rasoul Yousefi
authors.initials: RY
authors.lastname: Mashouf
authors.affiliation: Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. AND Department of Microbiology, Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Arabestani
mesh_terms: D018829:Adhesins, Bacterial / Q000032:analysis / Q000235:genetics; D000328:Adult; D064007:Ataxia Telangiectasia Mutated Proteins / Q000032:analysis / Q000235:genetics; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics*; D010450:Endopeptidases / Q000032:analysis / Q000235:genetics; D005260:Female; D006460:Hemolysin Proteins / Q000032:analysis / Q000235:genetics; D006801:Humans; D009479:Neuropeptides / Q000032:analysis / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011237:Predictive Value of Tests; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis* / Q000382:microbiology; D011263:Pregnancy Trimester, Third; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013290:Streptococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013292:Streptococcus agalactiae / Q000235:genetics*; D014621:Vagina / Q000382:microbiology; D055815:Young Adult
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; C039092:CAMP protein, Streptococcus; D006460:Hemolysin Proteins; D009479:Neuropeptides; D012336:RNA, Ribosomal, 16S; C041307:small cardioactive peptide B; D064007:Ataxia Telangiectasia Mutated Proteins; D010450:Endopeptidases; C053088:C5a peptidase
keywords: Polymerase chain reaction; Pregnancy; Streptococcus agalactiae