title: Orcinol derivative compound with antioxidant properties protects Langerhans islets against streptozotocin damage.
abstract: OBJECTIVE
  To design and synthesize an orcinol derivative compound, 3-formyl-2,4-dihydroxy-5,6-dimethyl sodium benzoate (A4), as an antioxidant molecule and to test its effects on oxidative stress in an in-vitro model of apoptosis of pancreatic rat beta cells induced by streptozotocin (STZ).
  
  
  METHODS
  Scavenger properties of A4 were assessed using its capacity to capture the DPPH radical in vitro. Antiapoptotic properties of A4 were analysed by electron microscopy and TUNEL assay in rat pancreatic islets in a streptozotocin model.
  
  
  RESULTS
  The results show that A4 displays antioxidant activity in in-vitro assays and induced a significant reduction in STZ-induced beta cell apoptosis and low ultrastructural damage to cellular organelles in the rat pancreatic islets as evidenced by electronic microscopy, this effect could be attributed to its antioxidant activity in a similar manner than resveratrol.
  
  
  CONCLUSIONS
  The overall results indicate that the new orcinol derivative molecule displays both antioxidant and antiapoptotic effects and protect pancreatic beta cells against STZ damage.
authors.affiliation: Departamento de Biología de la Reproducción, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Tlalpan, Ciudad de México, Mexico.
authors.forename: Nancy M V
authors.initials: NM
authors.lastname: Sánchez-Lira
authors.affiliation: Departamento de Biología de la Reproducción, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Tlalpan, Ciudad de México, Mexico.
authors.forename: Angélica
authors.initials: A
authors.lastname: Morales-Miranda
authors.affiliation: División de Posgrado, Departamento de Química Orgánica, Facultad de Química, Universidad Nacional Autónoma de México, Coyoacán, Ciudad de México, Mexico.
authors.forename: Gustavo
authors.initials: G
authors.lastname: García de la Mora
authors.affiliation: Departamento de Patología, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Tlalpan, Ciudad de México, Mexico.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: León Contreras
authors.affiliation: Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, Coyoacán, Ciudad de México, Mexico.
authors.forename: Ignacio
authors.initials: I
authors.lastname: González-Sánchez
authors.affiliation: Departamento de Farmacia, Facultad de Ciencias, Universidad Nacional de Colombia, Bogotá, Colombia.
authors.forename: Norma
authors.initials: N
authors.lastname: Valencia
authors.affiliation: Unidad de Investigación en Reproducción Humana, Facultad de Química, Instituto Nacional de Perinatología, Ciudad de México, Mexico.
authors.forename: Marco
authors.initials: M
authors.lastname: Cerbón
authors.affiliation: Departamento de Biología de la Reproducción, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Tlalpan, Ciudad de México, Mexico.
authors.forename: Sumiko
authors.initials: S
authors.lastname: Morimoto
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D050417:Insulin-Secreting Cells / Q000187:drug effects; D007515:Islets of Langerhans / Q000187:drug effects*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents; D051381:Rats; D017208:Rats, Wistar; D012118:Resorcinols / Q000494:pharmacology*; D013311:Streptozocin / Q000008:administration & dosage*
chemical_list: D000975:Antioxidants; D020011:Protective Agents; D012118:Resorcinols; C005282:orcinol; D013311:Streptozocin
keywords: 
  TUNEL
  ; cellular morphology; oxidative stress; pancreatic beta cells; rat