title: An isoform of Taiman that contains a PRD-repeat motif is indispensable for transducing the vitellogenic juvenile hormone signal in Locusta migratoria.
abstract: Taiman (Tai) has been recently identified as the dimerizing partner of juvenile hormone (JH) receptor, Methoprene-tolerant (Met). However, the role of Tai isoforms in transducing vitellogenic signal of JH has not been determined. In this study, we show that the migratory locust Locusta migratoria has two Tai isoforms, which differ in an INDEL-1 domain with the PRD-repeat motif rich in histidine and proline at the C-terminus. Tai-A with the INDEL-1 is expressed at levels about 50-fold higher than Tai-B without the INDEL-1 in the fat body of vitellogenic adult females. Knockdown of Tai-A but not Tai-B results in a substantial reduction of vitellogenin expression in the fat body accompanied by the arrest of ovarian development and oocyte maturation, similar to that caused by depletion of both Tai isoforms. Either Tai-A or Tai-B combined with Met can induce target gene transcription in response to JH, but Tai-A appears to mediate a significantly higher transactivation. Our data suggest that the INDEL-1 domain plays a critical role in Tai function during reproduction as Tai-A appears be more active than Tai-B in transducing the vitellogenic JH signal in L. migratoria.
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Plant Stress Biology, School of Life Sciences, Henan University, Kaifeng 475004, China.
authors.forename: Libin
authors.initials: L
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Plant Stress Biology, School of Life Sciences, Henan University, Kaifeng 475004, China.
authors.forename: Jiasheng
authors.initials: J
authors.lastname: Song
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Le
authors.initials: L
authors.lastname: Kang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Plant Stress Biology, School of Life Sciences, Henan University, Kaifeng 475004, China. Electronic address: szhou@henu.edu.cn.
authors.forename: Shutang
authors.initials: S
authors.lastname: Zhou
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005216:Fat Body / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D019476:Insect Proteins / Q000378:metabolism*; D007605:Juvenile Hormones / Q000378:metabolism*; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism; D048188:Locusta migratoria / Q000378:metabolism*; D008969:Molecular Sequence Data; D009865:Oocytes / Q000254:growth & development; D020033:Protein Isoforms / Q000378:metabolism; D015398:Signal Transduction; D014818:Vitellogenesis
chemical_list: D019476:Insect Proteins; D007605:Juvenile Hormones; D051741:Kruppel-Like Transcription Factors; D020033:Protein Isoforms
keywords: INDEL-1/PRD motif; Juvenile hormone; Oocyte maturation; Taiman isoforms; Vitellogenesis