title: Significance of 3'UTR and Pathogenic Haplotype in Glucose-6-Phosphate Deficiency.
abstract: OBJECTIVE
  To determine the polymorphisms in the 3' untranslated region (3'UTR) of the G6PD gene and analyze the specific SNPs or haplotypes that may affect messenger RNA (mRNA) secondary structure by in silico analysis.
  
  
  METHODS
  We studied the 3'UTR polymorphisms in 107 healthy subjects living in Thailand (ethnicities unknown). The haplotype was analyzed using Haploview software. mRNA secondary structure and microRNA binding of the G6PD gene were predicted by use of the CLC Main Workbench 6.9 and RegRNA 2.0 programs.
  
  
  RESULTS
  The results revealed 1 new variant in the 3'UTR of the G6PD gene (c.*99A > G). Haplotype ATCG showed significance by association-test results that yielded a high odds ratio (OR, 7.90; 95% confidence interval [CI], 3.15-19.81). Moreover, this haplotype affected G6PD mRNA secondary structure changes and microRNA binding via in silico analysis.
  
  
  CONCLUSIONS
  These results suggest that the haplotype ATCG is associated with reduction of G6PD enzyme expression, G6PD mRNA secondary structure changes, and microRNA binding via in silico analysis.
authors.affiliation: Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Mallika
authors.initials: M
authors.lastname: Chaowanathikhom
authors.affiliation: Department of Clinical Microscopy, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand.
authors.forename: Pornlada
authors.initials: P
authors.lastname: Nuchnoi
authors.affiliation: Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand nantadao@gmail.com.
authors.forename: Duangdao
authors.initials: D
authors.lastname: Palasuwan
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D000328:Adult; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000737:chemistry / Q000235:genetics / Q000648:ultrastructure; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D009690:Nucleic Acid Conformation; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D055815:Young Adult
chemical_list: D020413:3' Untranslated Regions; D012333:RNA, Messenger; D005954:Glucosephosphate Dehydrogenase
keywords: 3′UTR; G6PD; haplotype; mRNA secondary structure; malaria