title: Diagnosis of Strongyloides stercoralis by morphological characteristics combine with molecular biological methods.
abstract: Strongyloidiasis is one of the neglected tropical diseases caused by infection with the nematode Strongyloides genus and distributed worldwide. Strongyloidiasis can be fatal in immunosuppressed patients induced hyperinfection or disseminated strongyloidiasis. Unfortunately, until now, due to the unspecific clinical symptom in infected individuals and the low sensitivity diagnosis of strongyloidiasis, many patients were misdiagnosed every year. Furthermore, the larvae of the Strongyloides stercoralis (S. stercoralis) is similar to other nematodes such as hookworm, Trichostrongylus increased the difficulty of diagnosis. In this case, the patient is a 63-year-old male person, who had a nearly 30 years medical history of asthma and emphysema, and 4-5-year medical history of diabetes. The sputum examination found some parasite larvae, then we identify the larvae using clinical observation and morphological characteristics combine with examined cytochrome oxidase subunit 1 (COX1) and 18S rRNA genes by PCR, sequence analysis and finally classified by phylogenetic analysis, the larvae were diagnosed as S. stercoralis. Our results showed that diagnosis with strongyloidiasis by morphological characteristics combine with molecular biological methods can improve the sensitive of diagnosis and provide a final diagnosis for the disease in the clinics.
authors.affiliation: Department of Parasitology of Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.|Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of Education, Guangzhou, 510080, China.
authors.forename: Li-Fu
authors.initials: LF
authors.lastname: Wang
authors.affiliation: Department of Parasitology of Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.|Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of Education, Guangzhou, 510080, China.
authors.forename: Lian
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Parasitology of Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.|Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of Education, Guangzhou, 510080, China.
authors.forename: Shi-Qi
authors.initials: SQ
authors.lastname: Luo
authors.affiliation: Department of Parasitology of Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.|Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of Education, Guangzhou, 510080, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xie
authors.affiliation: Department of Pancreatobiliary Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Parasitology of Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China. wuzhd@mail.sysu.edu.cn.|Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of Education, Guangzhou, 510080, China. wuzhd@mail.sysu.edu.cn.
authors.forename: Zhong-Dao
authors.initials: ZD
authors.lastname: Wu
authors.affiliation: Department of Parasitology of Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China. sunxi2@mail.sysu.edu.cn.|Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of Education, Guangzhou, 510080, China. sunxi2@mail.sysu.edu.cn.
authors.forename: Xi
authors.initials: X
authors.lastname: Sun
mesh_terms: D000818:Animals; D001249:Asthma / Q000150:complications; D001483:Base Sequence; D051545:Cyclooxygenase 1 / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D003920:Diabetes Mellitus; D003937:Diagnosis, Differential; D004646:Emphysema / Q000150:complications; D006801:Humans; D016867:Immunocompromised Host; D007814:Larva / Q000469:parasitology; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012463:Saliva / Q000469:parasitology*; D017422:Sequence Analysis, DNA; D017171:Strongyloides stercoralis / Q000033:anatomy & histology* / Q000235:genetics*; D013322:Strongyloidiasis / Q000175:diagnosis* / Q000401:mortality / Q000469:parasitology; D014253:Trichostrongylosis / Q000175:diagnosis; D014254:Trichostrongylus
chemical_list: D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S; D051545:Cyclooxygenase 1
keywords: 18S rRNA; COX1; Molecular biological methods; Morphological characteristics; Strongyloides stercoralis; Strongyloidiasis