title: Permissive, in vitro replication of hepatitis B virus genotype E.
abstract: A cloned stable cell line, HepG2-HBVE6, was established following transfection of HepG2 cells with a retroviral plasmid into which a 1.1-fold genomic construct of hepatitis B virus (HBV) belonging to genotype E (HBV/E) was inserted. The cell line retains the entire HBV/E insert, and produces episomal HBV DNA. It expresses HBV pregenomic, preS1 and preS2/S transcripts, and sheds hepatitis B surface and e antigens as well as structures resembling HBV-subviral and Dane particles. The HepG2-HBVE6 cell line, in permitting recapitulation of the HBV life cycle, may be used for studying viral characteristics, therapeutic and preventative outcomes and for preparing reagents specific to HBV genotype E.
authors.affiliation: Division of Viral Hepatitis, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: jixinjl@gmail.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Ji
authors.affiliation: Division of Viral Hepatitis, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: fxu6@cdc.gov.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Zafrullah
authors.affiliation: Division of Viral Hepatitis, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: kue6@cdc.gov.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Wiese
authors.affiliation: Division of Viral Hepatitis, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: zdyo@cdc.gov.
authors.forename: Tonya
authors.initials: T
authors.lastname: Hayden-Mixon
authors.affiliation: Division of Viral Hepatitis, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: gzf7@cdc.gov.
authors.forename: Joseph C
authors.initials: JC
authors.lastname: Forbi
authors.affiliation: Division of Viral Hepatitis, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: teocg1@hotmail.com.
authors.forename: Chong-Gee
authors.initials: CG
authors.lastname: Teo
authors.affiliation: Division of Viral Hepatitis, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: mup3@cdc.gov.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Purdy
mesh_terms: D002460:Cell Line; D005838:Genotype; D006515:Hepatitis B virus / Q000235:genetics / Q000502:physiology*; D022781:Hepatocytes / Q000821:virology; D006801:Humans; D014779:Virus Replication
keywords: HepG2 cells; Hepadnavirus; Recombinant DNA; Viral replication