title: [Clonal relationship and detection of blaKPC gene in strains of Klebsiella pneumoniae resistant to carbapenems, at a hospital in Venezuela].
abstract: In order to study the clonal relationship and blaKPC gene detection in clinical isolates of Klebsiella pneumoniae resistant to carbapenems, we analyzed 22 clinical isolates of K. pneumoniae with resistance to imipenem and/ or meropenem, isolated in the laboratory of bacteriology at the University Hospital "Antonio Patricio de Alcalá" (HUAPA) from the Cumana city, Sucre state, Venezuela, for a period of five consecutive years. Susceptibility to different antimicrobials was determined, and the presence of carbapenemases was detected by modified Hodge method, phenyl boronic acid synergy and combination discs. blaKPC gene detection was conducted by polymerase chain reaction and the clonal relationship was determined by pulsed field electrophoresis. High rates of antimicrobial resistance were found, five strains were negative, at least one phenotypic method, and all carried the blaKPC gene. Clonal spread was observed only in the intensive care unit (ICU), while in other services, polyclonality was found. We concluded that blaKPC gene is present in K. pneumoniae strains resistant to carbapenems isolated in the HUAPA and clonal spread it was only in the ICU.
authors.affiliation: Laboratorio de Bacteriología, Hospital Universitario Antonio Patricio de Alcalá, Cumaná, Venezuela.
authors.forename: Dianny
authors.initials: D
authors.lastname: Martínez
authors.affiliation: Postgrado en Biología Aplicada, Universidad de Oriente, Cumaná, Venezuela.
authors.forename: Yasmina
authors.initials: Y
authors.lastname: Araque
authors.affiliation: Laboratorio de Genética Molecular, Instituto de Biomedicina y Ciencias Aplicadas Dra. Susan Tai, Cumaná, Venezuela.
authors.forename: Hectorina
authors.initials: H
authors.lastname: Roduifo
authors.affiliation: Laboratorio de Bacteriología, Hospital Universitario Antonio Patricio de Alcalá, Cumaná, Venezuela.
authors.forename: Luisa
authors.initials: L
authors.lastname: Caña
authors.affiliation: Laboratorio de Bacteriología, Hospital Universitario Antonio Patricio de Alcalá, Cumaná, Venezuela.
authors.forename: José
authors.initials: J
authors.lastname: García
authors.affiliation: Laboratorio de Bacteriología, Hospital Universitario Antonio Patricio de Alcalá, Cumaná, Venezuela.
authors.forename: Diorelis
authors.initials: D
authors.lastname: Gonzáiez
authors.affiliation: Laboratorio de Bacteriología, Hospital Universitario Antonio Patricio de Alcalá, Cumaná, Venezuela.
authors.forename: Lucy
authors.initials: L
authors.lastname: Rodríguez
authors.affiliation: Laboratorio de Genética Molecular, Instituto de Biomedicina y Ciencias Aplicadas Dra. Susan Tai, Cumaná, Venezuela.
authors.forename: Marcos
authors.initials: M
authors.lastname: De Donato
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D015780:Carbapenems / Q000494:pharmacology*; D002999:Clone Cells; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D014687:Venezuela; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D004269:DNA, Bacterial; C434283:beta-lactamase KPC-1; D001618:beta-Lactamases