title: Vascular Reactivity of Isolated Aorta to Study the Angiotensin-(1-7) Actions.
abstract: Since the early 1950s, vascular reactivity using isolated vessel rings has been a useful and efficient model for physiological and pharmacological studies. This experimental model was utilized in the milestone study of Dr. Robert Furchgott to discover the endothelium-derived relaxation factor (EDRF) nitric oxide (NO), opening new avenues and scientific perspectives in the vascular pathophysiology. Moreover, the isolated vessel ring preparation had an important contribution to understand many vasoactive systems. Here, we described the isolated aorta technique and pitfalls about the use of angiotensin-(1-7) peptide in this preparation.
authors.affiliation: National Institute of Science and Technology in Nanobiopharmaceutics, Gerais, Brazil.|Department of Physiology and Biophysics, Biological Science Institute, Federal University of Minas Gerais, Av. Pres. Antonio Carols 6627, Pampulha, Belo Horizonte, MG, 31270-901, Brazil.
authors.forename: Roberto Q
authors.initials: RQ
authors.lastname: Lautner
authors.affiliation: National Institute of Science and Technology in Nanobiopharmaceutics, Gerais, Brazil.|Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
authors.forename: Rodrigo A
authors.initials: RA
authors.lastname: Fraga-Silva
authors.affiliation: National Institute of Science and Technology in Nanobiopharmaceutics, Gerais, Brazil.|Department of Morphology, Biological Science Institute, Federal University of Minas Gerais, Gerais, Brazil.
authors.forename: Anderson J
authors.initials: AJ
authors.lastname: Ferreira
authors.affiliation: National Institute of Science and Technology in Nanobiopharmaceutics, Gerais, Brazil. robsonsant@gmail.com.|Department of Physiology and Biophysics, Biological Science Institute, Federal University of Minas Gerais, Av. Pres. Antonio Carols 6627, Pampulha, Belo Horizonte, MG, 31270-901, Brazil. robsonsant@gmail.com.
authors.forename: Robson A S
authors.initials: RA
authors.lastname: Santos
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000803:Angiotensin I / Q000494:pharmacology*; D000804:Angiotensin II / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001011:Aorta / Q000000981:diagnostic imaging*; D066298:In Vitro Techniques; D051379:Mice; D010446:Peptide Fragments / Q000494:pharmacology*; D010656:Phenylephrine / Q000494:pharmacology; D051381:Rats; D014664:Vasodilation / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: C092006:7-Ala-angiotensin (1-7); D010446:Peptide Fragments; D014665:Vasodilator Agents; D000804:Angiotensin II; D010656:Phenylephrine; D000803:Angiotensin I; C118790:angiotensin I (1-7); D000109:Acetylcholine
keywords: A-779; Angiotensin-(1-7); Aortic rings; D-Pro7-Angiotensin-(1-7); Endothelium; Vascular reactivity; Vasoconstriction; Vasodilation