title: Finding host targets for HIV therapy.
abstract: A CRISPR screen conducted in a CD4+T cell leukemia line has identified host factors required for HIV infection but dispensable for cellular survival. The results highlight sulfation on the HIV co-receptor CCR5 and cellular aggregation as potential targets for therapeutic intervention.
authors.affiliation: Departments of Genetics and Chemistry, Engineering and Medicine for Human Health (ChEM-H), Stanford University, Stanford, California, USA.
authors.forename: C Kimberly
authors.initials: CK
authors.lastname: Tsui
authors.affiliation: Departments of Genetics and Chemistry, Engineering and Medicine for Human Health (ChEM-H), Stanford University, Stanford, California, USA.
authors.forename: Amita
authors.initials: A
authors.lastname: Gupta
authors.affiliation: Departments of Genetics and Chemistry, Engineering and Medicine for Human Health (ChEM-H), Stanford University, Stanford, California, USA.
authors.forename: Michael C
authors.initials: MC
authors.lastname: Bassik
mesh_terms: D015703:Antigens, CD / Q000235:genetics* / Q000276:immunology; D015704:CD4 Antigens / Q000235:genetics* / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology; D002448:Cell Adhesion; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics* / Q000276:immunology; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D005326:Fetal Proteins / Q000235:genetics* / Q000276:immunology; D005786:Gene Expression Regulation; D015658:HIV Infections / Q000276:immunology / Q000473:pathology / Q000821:virology; D015497:HIV-1 / Q000502:physiology*; D057166:High-Throughput Screening Assays; D054884:Host-Pathogen Interactions; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics* / Q000276:immunology; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000276:immunology; D058990:Molecular Targeted Therapy; D019713:Receptors, CCR5 / Q000235:genetics* / Q000276:immunology; D000076988:Sulfate Transporters; D015238:Sulfotransferases / Q000235:genetics* / Q000276:immunology; D053586:Virus Internalization; D020410:Activated-Leukocyte Cell Adhesion Molecule
chemical_list: D015703:Antigens, CD; D015704:CD4 Antigens; D015816:Cell Adhesion Molecules, Neuronal; D005326:Fetal Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D019713:Receptors, CCR5; D000076988:Sulfate Transporters; D015238:Sulfotransferases; C105613:ALCAM protein, human; D020410:Activated-Leukocyte Cell Adhesion Molecule; C584933:CCR5 protein, human; C476248:SLC35B2 protein, human; C114740:TPST2 protein, human