title: Translocation of Bacillus thuringiensis in Phaseolus vulgaris tissues and vertical transmission in Arabidopsis thaliana.
abstract: OBJECTIVE
  To demonstrate the ability of Bacillus thuringiensis to penetrate as spore-crystal complex to the internal tissues of bean plants, and keep its insecticidal activity. To test the vertical transmission of the spore-crystal complex in Arabidopsis thaliana.
  
  
  RESULTS
  The experimental strain was transformed with the pMUTIN-gfp plasmid which labelled the spores of B. thuringiensis HD-73 with the GFP protein. Once the rhizosphere of the bean plants was inoculated with the labelled strain, the bacterium was recovered from leaves, stems, and petioles. Furthermore, toxicity of treated plants was significantly higher than control plants when bio-assayed on cabbage looper larvae. The labelled strain was recovered from the dead insects. When the rhizosphere of A. thaliana plants was inoculated with the labelled strain, mature seeds from these plants were surface-sterilized and grown under in vitro conditions. The labelled strain was recovered from the seedlings.
  
  
  CONCLUSIONS
  We showed that B. thuringiensis subsp. kurstaki (HD-73) in the rhizosphere can translocate to upper tissues of bean plants, and keep its insecticidal activity. Transmission of the labelled B. thuringiensis strain passed to the next generation of A. thaliana.
  
  
  CONCLUSIONS
  The role of B. thuringiensis as a potential facultative endophyte bacterium and the possible biotechnological repercussions are discussed.
authors.affiliation: Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Irapuato, Gto, Mexico.
authors.forename: R
authors.initials: R
authors.lastname: García-Suárez
authors.affiliation: Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Irapuato, Gto, Mexico.
authors.forename: L A
authors.initials: LA
authors.lastname: Verduzco-Rosas
authors.affiliation: División de Ciencias de la Vida, Departamento de Alimentos, Universidad de Guanajuato, Irapuato, Gto, Mexico.
authors.forename: M C
authors.initials: MC
authors.lastname: Del Rincón-Castro
authors.affiliation: Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Irapuato, Gto, Mexico.
authors.forename: J P
authors.initials: JP
authors.lastname: Délano-Frier
authors.affiliation: Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Irapuato, Gto, Mexico.
authors.forename: J E
authors.initials: JE
authors.lastname: Ibarra
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000382:microbiology*; D001413:Bacillus thuringiensis / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics / Q000633:toxicity*; D004731:Endotoxins / Q000032:analysis / Q000235:genetics / Q000633:toxicity*; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D006460:Hemolysin Proteins / Q000032:analysis / Q000235:genetics / Q000633:toxicity*; D007306:Insecticides / Q000032:analysis; D007814:Larva / Q000382:microbiology; D009036:Moths / Q000254:growth & development / Q000382:microbiology; D027805:Phaseolus / Q000382:microbiology*; D058441:Rhizosphere
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D049452:Green Fluorescent Proteins
keywords: 
  kurstaki
  ; cabbage looper; particle translocation; rhizosphere; vascular tissue