title: Techniques for the Evaluation of the Genetic Expression, Intracellular Storage, and Secretion of Polypeptide Hormones with Special Reference to the Natriuretic Peptides (NPs).
abstract: Techniques developed over the years in our laboratory for the study of tissue expression, storage, and secretion of the cardiac hormones ANF, BNP, and CNP are described below. They have proven highly reliable in our hands when the steps outlined are followed as described. Given the generic nature of the procedures, these should be applicable to other polypeptides.
authors.affiliation: Faculty of Medicine, Department of Pathology and Laboratory Medicine, Ottawa Heart Institute, University of Ottawa, Ottawa, ON, Canada, K1H 8M5. adebold@bell.net.|The Cardiovascular Endocrinology Laboratory, Ottawa Heart Institute, 40 Ruskin Street, Ottawa, ON, Canada, K1Y 4W7. adebold@bell.net.
authors.forename: Adolfo J
authors.initials: AJ
authors.lastname: de Bold
authors.affiliation: Faculty of Medicine, Department of Pathology and Laboratory Medicine, Ottawa Heart Institute, University of Ottawa, Ottawa, ON, Canada, K1H 8M5.|The Cardiovascular Endocrinology Laboratory, Ottawa Heart Institute, 40 Ruskin Street, Ottawa, ON, Canada, K1Y 4W7.
authors.forename: Mercedes L
authors.initials: ML
authors.lastname: de Bold
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D020097:Natriuretic Peptide, Brain / Q000032:analysis; D045265:Natriuretic Peptides / Q000032:analysis*; D036361:Peptide Hormones / Q000032:analysis*; D011863:Radioimmunoassay
chemical_list: D045265:Natriuretic Peptides; D036361:Peptide Hormones; D020097:Natriuretic Peptide, Brain
keywords: HPLC; Immunocytochemistry; In situ hybridization; Natriuretic peptides; PCR; Peptide isolation; Radioimmunoassay