title: Additive effects of the herbicide glyphosate and elevated temperature on the branched coral Acropora formosa in Nha Trang, Vietnam.
abstract: The combined effects of the herbicide glyphosate and elevated temperature were studied on the tropical staghorn coral Acropora formosa, in Nha Trang bay, Vietnam. The corals were collected from two different reefs, one close to a polluted fish farm and one in a marine-protected area (MPA). In the laboratory, branches of the corals were exposed to the herbicide glyphosate at ambient (28 °C) and at 3 °C elevated water temperatures (31 °C). Effects of herbicide and elevated temperature were studied on coral bleaching using photography and digital image analysis (new colorimetric method developed here based on grayscale), chlorophyll a analysis, and symbiotic dinoflagellate (Symbiodinium, referred to as zooxanthellae) counts. All corals from the MPA started to bleach in the laboratory before they were exposed to the treatments, indicating that they were very sensitive, as opposed to the corals collected from the more polluted site, which were more tolerant and showed no bleaching response to temperature increase or herbicide alone. However, the combined exposure to the stressors resulted in significant loss of color, proportional to loss in chlorophyll a and zooxanthellae. The difference in sensitivity of the corals collected from the polluted site versus the MPA site could be explained by different symbiont types: the resilient type C3u and the stress-sensitive types C21 and C23, respectively. The additive effect of elevated temperatures and herbicides adds further weight to the notion that the bleaching of coral reefs is accelerated in the presence of multiple stressors. These results suggest that the corals in Nha Trang bay have adapted to the ongoing pollution to become more tolerant to anthropogenic stressors, and that multiple stressors hamper this resilience. The loss of color and decrease of chlorophyll a suggest that bleaching is related to concentration of chloro-pigments. The colorimetric method could be further fine-tuned and used as a precise, non-intrusive tool for monitoring coral bleaching in situ.
authors.affiliation: Department of Ecology, Environment and Plant Sciences (DEEP), Stockholm University, 106 91, Stockholm, Sweden.
authors.forename: C
authors.initials: C
authors.lastname: Amid
authors.affiliation: Department of Ecology, Environment and Plant Sciences (DEEP), Stockholm University, 106 91, Stockholm, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Olstedt
authors.affiliation: Department of Ecology, Environment and Plant Sciences (DEEP), Stockholm University, 106 91, Stockholm, Sweden.
authors.forename: J S
authors.initials: JS
authors.lastname: Gunnarsson
authors.affiliation: Institute of Oceanography (IO), Nha Trang, Vietnam.
authors.forename: H
authors.initials: H
authors.lastname: Le Lan
authors.affiliation: Institute of Oceanography (IO), Nha Trang, Vietnam.
authors.forename: H
authors.initials: H
authors.lastname: Tran Thi Minh
authors.affiliation: Department of Aquatic Ecology and Water Quality Management, Wageningen University, P.O. Box 47, 6700 AA, Wageningen, The Netherlands.|Alterra, Wageningen University and Research Centre, P.O. Box 47, 6700 AA, Wageningen, The Netherlands.
authors.forename: P J
authors.initials: PJ
authors.lastname: Van den Brink
authors.affiliation: Department of Ecology, Environment and Plant Sciences (DEEP), Stockholm University, 106 91, Stockholm, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Hellström
authors.affiliation: Department of Ecology, Environment and Plant Sciences (DEEP), Stockholm University, 106 91, Stockholm, Sweden. michael.tedengren@su.se.
authors.forename: M
authors.initials: M
authors.lastname: Tedengren
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000502:physiology*; D000077194:Chlorophyll A / Q000737:chemistry*; D057894:Coral Reefs; D004141:Dinoflagellida / Q000737:chemistry*; D005998:Glycine / Q000031:analogs & derivatives* / Q000737:chemistry; D006540:Herbicides / Q000737:chemistry*; D013559:Symbiosis; D013624:Taiwan; D013696:Temperature; D014744:Vietnam; D000097797:Glyphosate
chemical_list: D000077194:Chlorophyll A; D005998:Glycine; D006540:Herbicides; D000097797:Glyphosate
keywords: Adaptation; Chlorophyll; Climate change; Coral bleaching; Digital image analysis; Genotype; Global warming; Pesticides; Tolerance; Zooxanthellae