title: [Amniotic Membrane Suspension and Autologous Serum - Are they Important for Wound Healing?]
abstract: Autologous serum (AS) and amniotic membrane transplantation (AMT) are used in the treatment of several ocular surface diseases. AMT is associated with a surgical intervention. Surgery could be avoided by using eye drops prepared from an amniotic membrane homogenate (AMH) or amniotic membrane suspension (AMS). EGF, bFGF, IL-6 and IL-8 were detected in AMS. However, EGF and bFGF concentrations in AMS were about 1.7-17× lower than in AMH, and IL-6 and IL-8 could not be detected in AMH. 100 % AMS, 15 and 30 % AMH significantly decreased proliferation of human corneal epithelial cells (HCECs) compared to controls (p = <0.002 for all), but 15 and 30 % AMS did not affect proliferation. Migration increased significantly compared to controls with 15 and 30 % AMS (p < 0.001), but did not change significantly with 15 or 30 % AMH (p = 0.153 and p = 0.083). Proliferation of HCECs was significantly greater with 15 % AS than with 30 % AS (p < 0.001). HCEC migration was significantly greater with 30 % AS than with 5 % AS (p < 0.01). In summary, 15 and 30 % AMS and 15 and 30 % AS exhibit the best supportive effect on human corneal epithelial cells. Nevertheless, we always have to keep in mind that individual growth factor concentrations exhibit high inter-individual fluctuations in AS or AMS eye drops.
authors.affiliation: Klinik für Augenheilkunde, Universitätsklinikum des Saarlandes (UKS), Homburg/Saar.
authors.forename: T
authors.initials: T
authors.lastname: Stachon
authors.affiliation: Klinik für Augenheilkunde, Universitätsklinikum des Saarlandes (UKS), Homburg/Saar.
authors.forename: M-F
authors.initials: MF
authors.lastname: Wu
authors.affiliation: Klinik für Augenheilkunde, Universitätsklinikum des Saarlandes (UKS), Homburg/Saar.
authors.forename: M
authors.initials: M
authors.lastname: Bischoff
authors.affiliation: Klinik für Augenheilkunde, Universitätsklinikum des Saarlandes (UKS), Homburg/Saar.
authors.forename: M
authors.initials: M
authors.lastname: Huber
authors.affiliation: Institut für Experimentelle Ophthalmologie, Universität des Saarlandes, Homburg/Saar.
authors.forename: A
authors.initials: A
authors.lastname: Langenbucher
authors.affiliation: Klinik für Augenheilkunde, Universitätsklinikum des Saarlandes (UKS), Homburg/Saar.
authors.forename: B
authors.initials: B
authors.lastname: Seitz
authors.affiliation: Klinik für Augenheilkunde, Universitätsklinikum des Saarlandes (UKS), Homburg/Saar.|Klinik für Augenheilkunde, Semmelweis Universität, Budapest, Ungarn.
authors.forename: N
authors.initials: N
authors.lastname: Szentmáry
mesh_terms: D001684:Biological Dressings; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003316:Corneal Diseases / Q000628:therapy*; D019573:Epithelium, Corneal / Q000187:drug effects; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000008:administration & dosage; D009883:Ophthalmic Solutions; D044967:Serum; D016896:Treatment Outcome; D014945:Wound Healing / Q000502:physiology*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D009883:Ophthalmic Solutions