title: SWATH label-free proteomics analyses revealed the roles of oxidative stress and antioxidant defensing system in sclerotia formation of Polyporus umbellatus.
abstract: Understanding the initiation and maturing mechanisms is important for rational manipulating sclerotia differentiation and growth from hypha of Polyporus umbellatus. Proteomes in P. umbellatus sclerotia and hyphae at initial, developmental and mature phases were studied. 1391 proteins were identified by nano-liquid chromatograph-mass spectrometry (LC-MS) in Data Dependant Acquisition mode, and 1234 proteins were quantified successfully by Sequential Window Acquisition of all THeoretical fragment ion spectra-MS (SWATH-MS) technology. There were 347 differentially expressed proteins (DEPs) in sclerotia at initial phase compared with those in hypha, and the DEP profiles were dynamically changing with sclerotia growth. Oxidative stress (OS) in sclerotia at initial phase was indicated by the repressed proteins of respiratory chain, tricarboxylic acid cycle and the activation of glycolysis/gluconeogenesis pathways were determined based on DEPs. The impact of glycolysis/gluconeogenesis on sclerotium induction was further verified by glycerol addition assays, in which 5% glycerol significantly increased sclerotial differentiation rate and biomass. It can be speculated that OS played essential roles in triggering sclerotia differentiation from hypha of P. umbellatus, whereas antioxidant activity associated with glycolysis is critical for sclerotia growth. These findings reveal a mechanism for sclerotial differentiation in P. umbellatus, which may also be applicable for other fungi.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100193 P. R. China.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.affiliation: Pharmaceutical department of China-Japan Friendship Hospital, Beijing 100029 P. R. China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Tian
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100193 P. R. China.
authors.forename: Chunlan
authors.initials: C
authors.lastname: Wang
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100193 P. R. China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100193 P. R. China.
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Xing
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100193 P. R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Ling
authors.affiliation: Tianjin University of Science &Technology, Tianjin 300457, P. R. China.
authors.forename: Wanqiang
authors.initials: W
authors.lastname: Yin
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100193 P. R. China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Tian
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100193 P. R. China.
authors.forename: Zhixia
authors.initials: Z
authors.lastname: Meng
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P. R. China.
authors.forename: Jihui
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100193 P. R. China.
authors.forename: Shunxing
authors.initials: S
authors.lastname: Guo
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism; D063990:Gene Ontology; D005990:Glycerol / Q000494:pharmacology; D013058:Mass Spectrometry / Q000379:methods*; D008954:Models, Biological; D058977:Molecular Sequence Annotation; D025282:Mycelium / Q000187:drug effects / Q000378:metabolism; D009997:Osmotic Pressure; D018384:Oxidative Stress; D055492:Polyporus / Q000187:drug effects / Q000378:metabolism*; D025341:Principal Component Analysis; D060066:Protein Interaction Maps; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013194:Staining and Labeling
chemical_list: D000975:Antioxidants; D005656:Fungal Proteins; D020543:Proteome; D017382:Reactive Oxygen Species; D005990:Glycerol