title: Enhanced Spacer Length between Mannose Mimicking Shikimoyl and Quinoyl Headgroups and Hydrophobic Region of Cationic Amphiphile Increases Efficiency of Dendritic Cell Based DNA Vaccination: A Structure-Activity Investigation.
abstract: In the field of dendritic cell based genetic immunization, previously we showed that liposomes of cationic amphiphiles containing mannose-mimicking shikimoyl headgroup are promising DNA vaccine carriers for dendritic cell (DC) transfection. The present structure-activity study reports on the influence of spacer length (between mannose-mimicking headgroups and quaternary nitrogen centers) in modulating the DC-transfection efficiencies. Further, we report on the anti-melanoma immune response inducing properties of the promising cationic amphiphiles in syngeneic C57BL/6J mice under prophylactic settings.
authors.affiliation: Biomaterials Group, CSIR-Indian Institute of Chemical Technology , Hyderabad 500 007, India.
authors.forename: Chandrashekhar
authors.initials: C
authors.lastname: Voshavar
authors.affiliation: Biomaterials Group, CSIR-Indian Institute of Chemical Technology , Hyderabad 500 007, India.
authors.forename: Rakesh C R
authors.initials: RC
authors.lastname: Meka
authors.affiliation: Biomaterials Group, CSIR-Indian Institute of Chemical Technology , Hyderabad 500 007, India.
authors.forename: Sanjoy
authors.initials: S
authors.lastname: Samanta
authors.affiliation: Biomaterials Group, CSIR-Indian Institute of Chemical Technology , Hyderabad 500 007, India.
authors.forename: Srujan
authors.initials: S
authors.lastname: Marepally
authors.affiliation: Biomaterials Group, CSIR-Indian Institute of Chemical Technology , Hyderabad 500 007, India.|Academy of Scientific and Innovative Research (AcSIR) , Taramani, Chennai 600 113, India.
authors.forename: Arabinda
authors.identifier: 0000-0002-0734-8320
authors.initials: A
authors.lastname: Chaudhuri
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000008:administration & dosage* / Q000276:immunology / Q000627:therapeutic use; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry*; D005260:Female; D057927:Hydrophobic and Hydrophilic Interactions; D008297:Male; D008358:Mannose / Q000031:analogs & derivatives*; D008545:Melanoma / Q000276:immunology / Q000517:prevention & control*; D008810:Mice, Inbred C57BL; D013501:Surface-Active Agents / Q000737:chemistry; D014162:Transfection / Q000379:methods*; D014611:Vaccination / Q000379:methods; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology / Q000627:therapeutic use
chemical_list: D019496:Cancer Vaccines; D004337:Drug Carriers; D013501:Surface-Active Agents; D019444:Vaccines, DNA; D008358:Mannose