title: N-acylhomoserine lactone-regulation of genes mediating motility and pathogenicity in Pseudomonas syringae pathovar tabaci 11528.
abstract: Pseudomonas syringae pathovar tabaci 11528 (P. syringae 11528) is a phytopathogen that causes wild-fire disease in soybean and tobacco plants. It utilizes a cell density-dependent regulation system known as quorum sensing (QS). In its QS system, the psyI is responsible for the biosynthesis of N-acylhomoserine lactones (AHLs). By comparing the transcripts from P. syringae 11528 wild-type strain with those of the ΔpsyI mutant using RNA sequencing (RNA-seq) technology, 1118 AHL-regulated genes were identified in the transition from exponential to stationary growth phase. Numerous AHL-regulated genes involved in pathogenicity were negatively controlled, including genes linked to flagella, chemotaxis, pilus, extracellular polysaccharides, secretion systems, and two-component system. Moreover, gene ontology and pathway enrichment analysis revealed that the most pronounced regulation was associated with bacterial motility. Finally, phenotypic assays showed that QS-regulated traits were involved in epiphytic growth of pathogens and disease development in plants. These findings imply that the AHL-mediated QS system in P. syringae 11528 plays significant roles in distinct stages of interactions between plants and pathogens, including early plant colonization and late plant infection.
authors.affiliation: Research Center for Eco-Environment Sciences, Chinese Academy of Sciences, Beijing, China.|University of the Chinese Academy of Sciences, Beijing, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Cheng
authors.affiliation: Research Center for Eco-Environment Sciences, Chinese Academy of Sciences, Beijing, China.|University of the Chinese Academy of Sciences, Beijing, China.
authors.forename: Anzhou
authors.identifier: 0000-0001-6197-777X
authors.initials: A
authors.lastname: Ma
authors.affiliation: Research Center for Eco-Environment Sciences, Chinese Academy of Sciences, Beijing, China.|University of the Chinese Academy of Sciences, Beijing, China.
authors.forename: Jinxue
authors.initials: J
authors.lastname: Luo
authors.affiliation: Research Center for Eco-Environment Sciences, Chinese Academy of Sciences, Beijing, China.|University of the Chinese Academy of Sciences, Beijing, China.
authors.forename: Xuliang
authors.initials: X
authors.lastname: Zhuang
authors.affiliation: Research Center for Eco-Environment Sciences, Chinese Academy of Sciences, Beijing, China.|University of the Chinese Academy of Sciences, Beijing, China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhuang
mesh_terms: D054742:Acyl-Butyrolactones / Q000378:metabolism*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D008124:Locomotion; D010935:Plant Diseases / Q000382:microbiology; D044224:Pseudomonas syringae / Q000235:genetics* / Q000502:physiology*; D017422:Sequence Analysis, DNA; D017423:Sequence Analysis, RNA; D013025:Glycine max; D014026:Nicotiana; D014774:Virulence
chemical_list: D054742:Acyl-Butyrolactones
keywords: Pseudomonas syringae pathovar tabaci 11528; RNA sequencing; gene expression; quorum sensing; virulence traits