title: Enhanced Isolation and Release of Circulating Tumor Cells Using Nanoparticle Binding and Ligand Exchange in a Microfluidic Chip.
abstract: The detection of rare circulating tumor cells (CTCs) in the blood of cancer patients has the potential to be a powerful and noninvasive method for examining metastasis, evaluating prognosis, assessing tumor sensitivity to drugs, and monitoring therapeutic outcomes. In this study, we have developed an efficient strategy to isolate CTCs from the blood of breast cancer patients using a microfluidic immune-affinity approach. Additionally, to gain further access to these rare cells for downstream characterization, our strategy allows for easy detachment of the captured CTCs from the substrate without compromising cell viability or the ability to employ next generation RNA sequencing for the identification of specific breast cancer genes. To achieve this, a chemical ligand-exchange reaction was engineered to release cells attached to a gold nanoparticle coating bound to the surface of a herringbone microfluidic chip (NP-HBCTC-Chip). Compared to the use of the unmodifiedHBCTC-Chip, our approach provides several advantages, including enhanced capture efficiency and recovery of isolated CTCs.
authors.affiliation: Department of Chemistry, Sahmyook University , Seoul, 01795, Korea.
authors.forename: Myoung-Hwan
authors.identifier: 0000-0003-3095-3016
authors.initials: MH
authors.lastname: Park
authors.forename: Eduardo
authors.initials: E
authors.lastname: Reátegui
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Shannon N
authors.initials: SN
authors.lastname: Tessier
authors.forename: Keith H K
authors.initials: KH
authors.lastname: Wong
authors.forename: Anne E
authors.initials: AE
authors.lastname: Jensen
authors.forename: Vishal
authors.initials: V
authors.lastname: Thapar
authors.forename: David
authors.initials: D
authors.lastname: Ting
authors.forename: Mehmet
authors.initials: M
authors.lastname: Toner
authors.forename: Shannon L
authors.initials: SL
authors.lastname: Stott
authors.forename: Paula T
authors.identifier: 0000-0002-9835-192X
authors.initials: PT
authors.lastname: Hammond
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D005260:Female; D005455:Fluorescent Antibody Technique; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008024:Ligands; D053768:Metal Nanoparticles / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques; D009360:Neoplastic Cells, Circulating / Q000737:chemistry*; D013499:Surface Properties; D059467:Transcriptome
chemical_list: D008024:Ligands; D006046:Gold