title: Halomonas aestuarii sp. nov., a moderately halophilic bacterium isolated from a tidal flat.
abstract: Strain Hb3Twas isolated from a tidal flat in Jeollabuk-do Gunsan, Republic of Korea. Cells were Gram-stain-negative, oxidase- and catalase-positive, rod-shaped and motile. The strain grew optimally at 25-35 °C, at pH 6.0-6.5 and with 3.0-10.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain Hb3Tbelonged to the genus Halomonas. Strain Hb3Twas related most closely to Halomonas ventosae Al12T(98.6 % 16S rRNA gene sequence similarity), Halomonas denitrificans M29T(98.6 %) and Halomonas saccharevitans AJ275T(98.4 %). Moreover, multilocus sequence analysis using the gyrB, rpoD and secA genes supported the phylogenetic position of strain Hb3T. The genomic G+C content of strain Hb3Twas 67.9 mol%. DNA-DNA hybridization values for strain Hb3Tversus H. ventosae Al12T, H. denitrificans M29Tand H. saccharevitans AJ275Twere 38.0, 54.5 and 47.4 %, respectively. The major quinone was ubiquinone Q-9 and the major fatty acids were C18 : 1ω7c, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0 and C19 : 0 cyclo ω8c. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, amino lipid, six unidentified phospholipids and an unidentified lipid comprised the polar lipid profile. On the basis of the data presented in this report, strain Hb3Trepresents a novel species of the genus Halomonas. The name Halomonas aestuarii sp. nov. is proposed for this novel species. The type strain is Hb3T(=KCTC 52253T=JCM 31415T).
authors.affiliation: Department of Biology, Jeju National University, Jejudaehak-ro 102, Jeju 63243, Republic of Korea.
authors.forename: Heyon-Woo
authors.initials: HW
authors.lastname: Koh
authors.affiliation: Department of Biology, Jeju National University, Jejudaehak-ro 102, Jeju 63243, Republic of Korea.
authors.forename: Sundas
authors.initials: S
authors.lastname: Rani
authors.affiliation: Freshwater Bioresources Utilization Division, Nakdonggang National Institute of Biological Resources, Donam 2-gil, Sangju 37242, Republic of Korea.|Present address: Geologic Environment Research Division, Korea Institute of Geoscience and Mineral Resources, 124 Gwahak-ro, Daejeon, 34132, Republic of Korea.
authors.forename: So-Jeong
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Division of Electron Microscopic Research, Korea Basic Science Institute, 169-148 Gwahak-ro, Yuseong-gu, Daejeon 34133, Republic of Korea.
authors.forename: Eunyoung
authors.initials: E
authors.lastname: Moon
authors.affiliation: Bioresources Culture Collection Division, Nakdonggang National Institute of Biological Resources, Donam 2-gil, Sangju 37242, Republic of Korea.
authors.forename: Seung Won
authors.initials: SW
authors.lastname: Nam
authors.affiliation: Department of Microbiology, Chungbuk National University, Chungdae-ro 1, Cheongju 28644, Republic of Korea.
authors.forename: Sung-Keun
authors.initials: SK
authors.lastname: Rhee
authors.affiliation: Department of Biology, Jeju National University, Jejudaehak-ro 102, Jeju 63243, Republic of Korea.
authors.forename: Soo-Je
authors.initials: SJ
authors.lastname: Park
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D019015:Geologic Sediments / Q000382:microbiology*; D020632:Halomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D058885:Multilocus Sequence Typing; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030571:ubiquinone 9
keywords: Halomonas aestuarii; halophilic; novel species; polyphasic; tidal flat