title: Graphene oxide-SiO2nanocomposite as the adsorbent for extraction and preconcentration of plant hormones for HPLC analysis.
abstract: In this research, a modified Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) method based on graphene oxide@SiO2(SiO2@GO) nanocomposite as adsorbent of dispersive solid-phase extraction (dSPE) combined with high performance liquid chromatography (HPLC) for the analysis of four plant hormones in different plants was established. The as-prepared SiO2@GO was characterized by scanning electron microscopy, transmission electron microscopy and infrared spectroscopy. The experimental conditions for dSPE, including the ratio of material to liquid, pH of sample, adsorption and desorption time, desorption temperature as well as desorption solution, were investigated. The detection limits for the analysis of indole-3-acetic acid, indole-3-butyric acid, 1-naphthylacetic acid and abscisic acid were achieved below 0.05μgmL-1. The established method was applied to the analysis of the plant hormones in fruits, vegetables and other food samples. The obtained results indicated that the method was sensitive, accurate, convenient and quick, which provided an alternative analytical approach for plant hormones in complex matrices.
authors.affiliation: Institute of Environmental and Analysis Science, School of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, Henan, China.
authors.forename: Xiaona
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute of Environmental and Analysis Science, School of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, Henan, China.
authors.forename: Jiahua
authors.initials: J
authors.lastname: Niu
authors.affiliation: Institute of Environmental and Analysis Science, School of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, Henan, China.
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute of Environmental and Analysis Science, School of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, Henan, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xiao
authors.affiliation: Institute of Environmental and Analysis Science, School of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, Henan, China. Electronic address: mhlu@henu.edu.cn.
authors.forename: Minghua
authors.initials: M
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University, Hong Kong SAR, China. Electronic address: zwcai@hkbu.edu.hk.
authors.forename: Zongwei
authors.initials: Z
authors.lastname: Cai
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D016014:Linear Models; D010937:Plant Growth Regulators / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D010937:Plant Growth Regulators; D012822:Silicon Dioxide; D006108:Graphite
keywords: Dispersive solid-phase extraction; Graphene oxide-SiO(2) nanocomposite; HPLC; Plant hormones; QuEChERS