title: In Vitro Analysis of Hypertensive Signal Transduction: Kinase Activation, Kinase Manipulation, and Physiologic Outputs.
abstract: Studying signal transduction in hypertension model systems in vitro will include several steps: (1) develop the cell culture model and induce hypertensive changes, (2) observe kinase activation, (3) manipulate signal transduction pathways, and (4) observe physiologic outputs. This chapter will provide the reader with overviews of the techniques our lab uses to inhibit signaling pathways with inhibitory RNAs and the outputs we use to monitor the effects.
authors.affiliation: Lewiz Katz School of Medicine, Temple University, 3500 Nth Board St, Philadephia, PA, 19140, USA.
authors.forename: Katherine J
authors.initials: KJ
authors.lastname: Elliott
authors.affiliation: Lewiz Katz School of Medicine, Temple University, 3500 Nth Board St, Philadephia, PA, 19140, USA. seguchi@temple.edu.
authors.forename: Satoru
authors.initials: S
authors.lastname: Eguchi
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001011:Aorta / Q000378:metabolism; D015153:Blotting, Western; D049109:Cell Proliferation / Q000235:genetics / Q000502:physiology; D002478:Cells, Cultured; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000235:genetics / Q000378:metabolism; D006973:Hypertension / Q000235:genetics / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000235:genetics; D010766:Phosphorylation / Q000235:genetics / Q000502:physiology; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology
chemical_list: D035683:MicroRNAs; D066246:ErbB Receptors; D011505:Protein-Tyrosine Kinases; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: Adenovirus; Angiotensin II; EGFR; ERK; Explant primary cell culture; MAP kinase; Signal transduction; Tyrosine kinase; VSMC; miRNA