title: Molecular epidemiology of pseudorabies virus in Yunnan and the sequence analysis of its gD gene.
abstract: Outbreaks of pseudorabies (PRs) have occurred in Yunnan, China, which caused significant economic loss. To determine the prevalence and origin of PR in Yunnan, especially among vaccinated pigs, overall 791 samples of blood, tissue, semen, and sera were analyzed by serological methods, PCR, and sequence analysis of gD gene. Detection with viral gI antibody or PCR showed that the yearly positive rates of PR virus (PRV) in Yunnan from 2010 to 2014 were 48.15, 21.26, 2.17, 5.22, and 0.35%, respectively, with an average of 15.43%. In general, the incidence declined through the period of 2010-2014 probably due to the application of PRV eradication strategies. A phylogenetic tree was constructed based on the complete sequence of gD gene, with all strains clustered into two independent clades, i.e., Asian and European-American clades. The virus isolates from Henan, Tianjin, Heilongjiang, Sichuan, Shandong, Fujian, Xinjiang, Hubei, Guangdong, and Yunnan fell into Asian group, which harbored South Korea isolate. Four Yunnan virus isolates together with South Korean Namyangju fell into in the European-American clade. It showed that PR was pandemic as there was not a clear clue about the geographical origin of the PRV isolates in China since 2010.
authors.affiliation: Department of Veterinary Medicine, College of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, Yunnan Province, China.
authors.forename: Chunlian
authors.initials: C
authors.lastname: Song
authors.affiliation: Department of Veterinary Medicine, College of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, Yunnan Province, China.
authors.forename: Libo
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of Veterinary Medicine, College of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, Yunnan Province, China.
authors.forename: Weibing
authors.initials: W
authors.lastname: Bai
authors.affiliation: Department of Veterinary Medicine, College of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, Yunnan Province, China.
authors.forename: Xingqin
authors.initials: X
authors.lastname: Zha
authors.affiliation: Department of Veterinary Medicine, College of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, Yunnan Province, China. yingefen0616@163.com.
authors.forename: Gefen
authors.initials: G
authors.lastname: Yin
authors.affiliation: Department of Veterinary Medicine, College of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, Yunnan Province, China. 1633992175@qq.com.
authors.forename: Xianghua
authors.initials: X
authors.lastname: Shu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000302:isolation & purification; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D003001:Cloning, Molecular; D004279:DNA, Viral; D004196:Disease Outbreaks; D005787:Gene Frequency; D011558:Herpesvirus 1, Suid / Q000235:genetics*; D017720:Molecular Epidemiology; D010802:Phylogeny; D011557:Pseudorabies / Q000821:virology*; D056910:Republic of Korea; D017421:Sequence Analysis; D012698:Serologic Tests; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000821:virology; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D014759:Viral Envelope Proteins; C488986:glycoprotein D, Feline herpesvirus 1
keywords: Phylogenetic tree; Pseudorabies virus; Yunnan; gD gene