title: Enzyme kinetics approach to assess biocatalyst inhibition and deactivation caused by [bmim][Cl] ionic liquid during cellulose hydrolysis.
abstract: The aim of this work was to study the inhibition and deactivation of commercial enzyme cocktail (Cellic® Htec2) in the presence of [bmim][Cl] ionic liquid employing model cellulosic substrate, carboxymethyl cellulose (CMC). It turned out from the experiments - relying on enzyme kinetics approach - that [bmim][Cl] could act as a competitive inhibitor. Furthermore, depending on the process conditions i.e. contact of enzyme solution with high concentration [bmim][Cl], severe biocatalyst inactivation should be also taken into account as a potential risk during the enzymatic cellulose hydrolysis even in as short process times as few minutes.
authors.affiliation: Research Institute on Bioengineering, Membrane Technology and Energetics, University of Pannonia, Egyetem ut 10, 8200 Veszprém, Hungary.
authors.forename: Nándor
authors.initials: N
authors.lastname: Nemestóthy
authors.affiliation: Research Institute on Bioengineering, Membrane Technology and Energetics, University of Pannonia, Egyetem ut 10, 8200 Veszprém, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Megyeri
authors.affiliation: Research Institute on Bioengineering, Membrane Technology and Energetics, University of Pannonia, Egyetem ut 10, 8200 Veszprém, Hungary.
authors.forename: Péter
authors.initials: P
authors.lastname: Bakonyi
authors.affiliation: Research Institute on Bioengineering, Membrane Technology and Energetics, University of Pannonia, Egyetem ut 10, 8200 Veszprém, Hungary.
authors.forename: Patrik
authors.initials: P
authors.lastname: Lakatos
authors.affiliation: Research Institute on Bioengineering, Membrane Technology and Energetics, University of Pannonia, Egyetem ut 10, 8200 Veszprém, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Koók
authors.affiliation: Department of Chemical and Biochemical Engineering, Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, Bratislava 81237, Slovakia.
authors.forename: Milan
authors.initials: M
authors.lastname: Polakovic
authors.affiliation: Research Institute on Bioengineering, Membrane Technology and Energetics, University of Pannonia, Egyetem ut 10, 8200 Veszprém, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Gubicza
authors.affiliation: Research Institute on Bioengineering, Membrane Technology and Energetics, University of Pannonia, Egyetem ut 10, 8200 Veszprém, Hungary. Electronic address: bako@almos.uni-pannon.hu.
authors.forename: Katalin
authors.initials: K
authors.lastname: Bélafi-Bakó
mesh_terms: D002266:Carboxymethylcellulose Sodium / Q000737:chemistry / Q000378:metabolism; D044602:Cellulases / Q000737:chemistry / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism; D006868:Hydrolysis; D007093:Imidazoles / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D007700:Kinetics; D012996:Solutions / Q000737:chemistry
chemical_list: D007093:Imidazoles; D052578:Ionic Liquids; D012996:Solutions; C502841:1-butyl-3-methylimidazolium chloride; D002482:Cellulose; D044602:Cellulases; D002266:Carboxymethylcellulose Sodium
keywords: Deactivation; Enzymatic hydrolysis; Inhibition; Ionic liquid; Lignocellulose; Pretreatment