title: Antigenic Cross-reactivity amongHaemonchus contortus, Oesophagostomum columbianum and Trichuris ovisof Goat.
abstract: BACKGROUND
  Cross antigenicity is the major problem in developing a reliable tool for immunodiagnosis and immunoprophylaxis of parasitic diseases. Mixed infection due to different types of gastrointestinal parasites is more common than single species infection under field condition.
  
  
  METHODS
  The present study was undertaken to detect antigenic cross-reactivity amongHaemonchus contortus, Oesophagostomum columbianumandTrichuris ovisof goats by SDS-PAGE and western blot analysis using hyperimmune sera (HIS) rose in rabbit separately against the antigens of the three nematode species.
  
  
  RESULTS
  Thirteen, 16 and 14 polypeptides in crude somatic antigen (CSAg) ofH. contortus(CSAg-Hc),O. columbianum(CSAg-Oc) andT. ovis(CSAg-To), respectively, were resolved in SDS PAGE analyses. It was revealed that 54 kDa peptide was shared byH.contortusandO. columbianum, whereas 47 kDa peptide was shared byO. columbianumandT. ovis. Western blot analyses revealed that three immunogenic polypeptides (MW 54, 49 and 42 kDa) in CSAg-Hc, five in CSAg-Oc (54, 47, 44, 38 and 35.5 kDa) and CSAg-To and five polypeptides (90, 51, 47, 39.5 and 31 kDa) in CSAg-To cross-reacted with the heterologous HIS. Four species-specific immunoreactive polypeptides (92, 85, 65 and 39 kDa) ofH. contortusand two (72 & 26 kDa) inO. columbianumwere also identified in the study.
  
  
  CONCLUSIONS
  The shared polypeptides and species-specific polypeptides might be evaluated as protective antigen and subsequently exploitation for developing immunodiagnostic and for immunoprophylactic tools of for these common nematode species.
authors.affiliation: Department of Veterinary Parasitology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kshudiram Bose Sarani, Kolkata, India.
authors.forename: Ruma
authors.initials: R
authors.lastname: Jas
authors.affiliation: Department of Veterinary Parasitology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kshudiram Bose Sarani, Kolkata, India.
authors.forename: Joydeb
authors.initials: J
authors.lastname: Ghosh
authors.affiliation: Department of Veterinary Parasitology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kshudiram Bose Sarani, Kolkata, India.
authors.forename: Kinsuk
authors.initials: K
authors.lastname: DAS
keywords: Antigenic profiles; Cross-reactivity; Haemonchus contortus; Oesophagostomum columbianum; Trichuris ovis