title: Effect of Proteolysis with Alkaline Protease Following High Hydrostatic Pressure Treatment on IgE Binding of Buckwheat Protein.
abstract: Buckwheat is a popular food material in many Asian countries and it contains major allergenic proteins. This study was performed to analyze the effects of hydrolysis with alkaline protease following high hydrostatic pressure (HHP) treatment on the IgE binding of buckwheat protein. Extracted buckwheat protein was treated with HHP at 600 MPa for 30 min and hydrolyzed with alkaline protease for 240 min. IgE binding was examined using an enzyme-linked immunosorbent assay (ELISA) with serum samples from 14 patients who were allergic to buckwheat. Depending on the serum samples, HHP treatment of buckwheat protein without enzymatic hydrolysis decreased the IgE binding by 8.9% to 73.2% or increased by 31% to 78%. The IgE binding of buckwheat protein hydrolyzed with alkaline protease decreased by 73.8% to 100%. The IgE binding of buckwheat protein hydrolyzed with alkaline protease following HHP treatment decreased by 83.8% to 100%. This suggested that hydrolysis with alkaline protease following HHP treatment could be applied to reduce the IgE binding of buckwheat protein.
authors.affiliation: the Dept. of Food Science and Engineering, Ewha Womans Univ, Seodaemun-gu, Seoul, 03760, Republic of Korea.
authors.forename: Chaeyoon
authors.initials: C
authors.lastname: Lee
authors.affiliation: the Dept. of Food Science and Engineering, Ewha Womans Univ, Seodaemun-gu, Seoul, 03760, Republic of Korea.
authors.forename: Wonhui
authors.initials: W
authors.lastname: Lee
authors.affiliation: Environmental Health Center for Atopic Diseases, Samsung Medical Center, School of Medicine, Sungkyunkwan Univ., Gangnam-gu, Seoul, 06351, Republic of Korea.
authors.forename: Youngshin
authors.initials: Y
authors.lastname: Han
authors.affiliation: the Dept. of Food Science and Engineering, Ewha Womans Univ, Seodaemun-gu, Seoul, 03760, Republic of Korea.
authors.forename: Sangsuk
authors.initials: S
authors.lastname: Oh
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000097:blood; D001208:Asia; D001426:Bacterial Proteins / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D004044:Dietary Proteins / Q000276:immunology / Q000378:metabolism*; D010450:Endopeptidases / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D019612:Fagopyrum / Q000737:chemistry / Q000276:immunology*; D005260:Female; D005511:Food Handling / Q000379:methods*; D005512:Food Hypersensitivity / Q000276:immunology; D006801:Humans; D006868:Hydrolysis; D006874:Hydrostatic Pressure; D007073:Immunoglobulin E / Q000097:blood*; D007223:Infant; D008297:Male; D010940:Plant Proteins / Q000276:immunology / Q000378:metabolism*; D059748:Proteolysis; D055815:Young Adult
chemical_list: D000485:Allergens; D001426:Bacterial Proteins; D004044:Dietary Proteins; D010940:Plant Proteins; D007073:Immunoglobulin E; D010450:Endopeptidases; C087822:alkaline protease
keywords: IgE binding; alkaline protease; buckwheat; high hydrostatic pressure; hydrolysis