title: Mutual effect of cerebral amyloid β and peripheral lymphocytes in cognitively normal older individuals.
abstract: We hypothesized that cerebral amyloid accumulation is reflected in the periphery in the pre-dementia stage and used flow cytometry to investigate the peripheral lymphocytes as an easily accessible biomarker to observe neuro-inflammation. We aimed to determine whether peripheral lymphocytes are related to the cortical amyloid burden or vice versa in cognitively normal older subjects.
  
  
  
  We applied [11C] Pittsburgh compound B (PiB)-positron emission tomography to 36 cognitively normal older individuals, and Aβ deposition was quantified by cortical binding potential (PiB-BPND). Blood samples were obtained, and lymphocyte subsets were evaluated. We examined differences between low and high PiB-BPNDgroups in the percentage of B cells, T cells, helper T cells, cytotoxic T cells, regulatory T cells, and natural killer cells.RESULTS: Subjects with high PiB-BPNDshowed significantly higher percentage of cytotoxic T cells (%CD3+). Correlation analysis revealed a significant relationship between the percentage of cytotoxic T cells and global cortical mean PiB-BPND. Hierarchical regression analyses showed that cytotoxic T cells were significantly related to the value of global cortical mean PiB-BPNDand vice versa.
  
  
  
  Our results indicated that a specific peripheral immune response, reflected in the increased ratio of cytotoxic T cells, could be regarded as a preclinical sign of AD and could be attributed to the Aβ neuropathological mechanism. Copyright © 2017 John Wiley & Sons, Ltd.
authors.affiliation: Department of Psychiatry, Nara Medical University, Kashihara, Japan.|Department of Investigative Radiology, National Cerebral and Cardiovascular Center, Suita, Japan.
authors.forename: Fumihiko
authors.identifier: 0000-0002-5981-5795
authors.initials: F
authors.lastname: Yasuno
authors.affiliation: Department of Neuropsychiatry, Osaka University Medical School, Suita, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kazui
authors.affiliation: Department of Neurology, National Cerebral and Cardiovascular Center, Suita, Japan.
authors.forename: Katsufumi
authors.initials: K
authors.lastname: Kajimoto
authors.affiliation: Department of Neurology, National Cerebral and Cardiovascular Center, Suita, Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Ihara
authors.affiliation: Department of Radiology, National Cerebral and Cardiovascular Center, Suita, Japan.
authors.forename: Naomi
authors.initials: N
authors.lastname: Morita
authors.affiliation: Department of Neurology, National Cerebral and Cardiovascular Center, Suita, Japan.|Department of Regenerative Medicine Research, Institute of Biomedical Research and Innovation, Kobe, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Taguchi
authors.affiliation: Department of Investigative Radiology, National Cerebral and Cardiovascular Center, Suita, Japan.
authors.forename: Akihide
authors.initials: A
authors.lastname: Yamamoto
authors.affiliation: Department of Psychiatry, Nara Medical University, Kashihara, Japan.
authors.forename: Kiwamu
authors.initials: K
authors.lastname: Matsuoka
authors.affiliation: Department of Psychiatry, Nara Medical University, Kashihara, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Takahashi
authors.affiliation: Department of Radiology, National Cerebral and Cardiovascular Center, Suita, Japan.
authors.forename: Jyoji
authors.initials: J
authors.lastname: Nakagawara
authors.affiliation: Department of Regenerative Medicine and Tissue Engineering, National Cerebral and Cardiovascular Center, Suita, Japan.
authors.forename: Masahito
authors.initials: M
authors.lastname: Tsuji
authors.affiliation: Department of Investigative Radiology, National Cerebral and Cardiovascular Center, Suita, Japan.
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Iida
authors.affiliation: Department of Psychiatry, Nara Medical University, Kashihara, Japan.
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Kishimoto
authors.affiliation: Department of Neurology, National Cerebral and Cardiovascular Center, Suita, Japan.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Nagatsuka
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D016229:Amyloid beta-Peptides / Q000097:blood*; D000814:Aniline Compounds / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D002540:Cerebral Cortex / Q000166:cytology*; D003071:Cognition / Q000502:physiology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008214:Lymphocytes / Q000166:cytology*; D008279:Magnetic Resonance Imaging / Q000379:methods; D008297:Male; D008875:Middle Aged; D049268:Positron-Emission Tomography / Q000379:methods; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology; D013844:Thiazoles / Q000378:metabolism
chemical_list: C475519:2-(4'-(methylamino)phenyl)-6-hydroxybenzothiazole; D016229:Amyloid beta-Peptides; D000814:Aniline Compounds; D015415:Biomarkers; D013844:Thiazoles
keywords: 11C-labeled Pittsburgh compound B ([11C]PiB); Alzheimer's disease (AD); CD8 T cells; amyloid β (Aβ); lymphocytes; positron emission tomography (PET)