title: Homoeologous recombination-based transfer and molecular cytogenetic mapping of powdery mildew-resistant gene Pm57 from Aegilops searsii into wheat.
abstract: Pm57, a novel resistant gene against powdery mildew, was transferred into common wheat from Ae. searsi and further mapped to 2Ss#1L at an interval of FL0.75 to FL0.87. Powdery mildew, caused by the fungus Blumeria graminis f. sp. tritici, is one of the most severe foliar diseases of wheat causing reduction in grain yield and quality. Host plant resistance is the most effective and environmentally safe approach to control this disease. Tests of a set of Chinese Spring-Ae. searsii (SsSs, 2n = 2x = 14) Feldman & Kislev ex K. Hammer disomic addition lines with a mixed isolate of the powdery mildew fungus identified a novel resistance gene(s), designed as Pm57, which was located on chromosome 2Ss#1. Here, we report the development of ten wheat-Ae. searsii recombinants. The wheat chromosomes involved in five of these recombinants were identified by FISH and SSR marker analysis and three of them were resistant to powdery mildew. Pm57 was further mapped to the long arm of chromosome 2Ss#1 at a fraction length interval of FL 0.75 to FL 0.87. The recombinant stocks T2BS.2BL-2Ss#1L 89-346 (TA5108) with distal 2Ss#1L segments of 28% and 89(5)69 (TA5109) with 33% may be useful in wheat improvement. The PCR marker X2L4g9p4/HaeIII was validated to specifically identify the Ae. searsii 2Ss#1L segment harboring Pm57 in T2BS.2BL-2Ss#1L against 16 wheat varieties and advanced breeding lines, and the development of more user-friendly KASP markers is underway.
authors.affiliation: College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China. wxliu2003@hotmail.com.
authors.forename: Wenxuan
authors.identifier: http://orcid.org/0000-0003-1227-0991
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Plant Pathology, Wheat Genetics Resource Center, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS, 66506-5502, USA.
authors.forename: Dal-Hoe
authors.initials: DH
authors.lastname: Koo
authors.affiliation: College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Xia
authors.affiliation: Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China.
authors.forename: Chunxin
authors.initials: C
authors.lastname: Li
authors.affiliation: College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Bai
authors.affiliation: Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China.
authors.forename: Yuli
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Plant Pathology, Wheat Genetics Resource Center, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS, 66506-5502, USA.
authors.forename: Bernd
authors.initials: B
authors.lastname: Friebe
authors.affiliation: Department of Plant Pathology, Wheat Genetics Resource Center, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS, 66506-5502, USA.
authors.forename: Bikram S
authors.initials: BS
authors.lastname: Gill
mesh_terms: D001203:Ascomycota; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D018744:DNA, Plant / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant; D005819:Genetic Markers; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000382:microbiology; D006109:Poaceae / Q000235:genetics*; D011995:Recombination, Genetic; D014908:Triticum / Q000235:genetics* / Q000382:microbiology
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers
keywords: Aegilops searsii; Blumeria tritici; FISH; GISH; Pm57; Triticum aestivum