title: Identification of Receptor Binding to the Biomolecular Corona of Nanoparticles.
abstract: Biomolecules adsorbed on nanoparticles are known to confer a biological identity to nanoparticles, mediating the interactions with cells and biological barriers. However, how these molecules are presented on the particle surface in biological milieu remains unclear. The central aim of this study is to identify key protein recognition motifs and link them to specific cell-receptor interactions. Here, we employed an immuno-mapping technique to quantify epitope presentations of two major proteins in the serum corona, low-density lipoprotein and immunoglobulin G. Combining with a purpose-built receptor expression system, we show that both proteins present functional motifs to allow simultaneous recognition by low-density lipoprotein receptor and Fc-gamma receptor I of the corona. Our results suggest that the "labeling" of nanoparticles by biomolecular adsorption processes allows for multiple pathways in biological processes in which they may be "mistaken" for endogenous objects, such as lipoproteins, and exogenous ones, such as viral infections.
authors.affiliation: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin , Belfield, Dublin 4, Ireland.
authors.forename: Sandra
authors.initials: S
authors.lastname: Lara
authors.affiliation: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin , Belfield, Dublin 4, Ireland.
authors.forename: Fatima
authors.initials: F
authors.lastname: Alnasser
authors.affiliation: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin , Belfield, Dublin 4, Ireland.
authors.forename: Ester
authors.identifier: 0000-0001-8870-5280
authors.initials: E
authors.lastname: Polo
authors.affiliation: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin , Belfield, Dublin 4, Ireland.
authors.forename: David
authors.initials: D
authors.lastname: Garry
authors.affiliation: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin , Belfield, Dublin 4, Ireland.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Lo Giudice
authors.affiliation: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin , Belfield, Dublin 4, Ireland.
authors.forename: Delyan R
authors.initials: DR
authors.lastname: Hristov
authors.affiliation: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin , Belfield, Dublin 4, Ireland.
authors.forename: Louise
authors.initials: L
authors.lastname: Rocks
authors.affiliation: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin , Belfield, Dublin 4, Ireland.
authors.forename: Anna
authors.identifier: 0000-0002-9339-0161
authors.initials: A
authors.lastname: Salvati
authors.affiliation: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin , Belfield, Dublin 4, Ireland.
authors.forename: Yan
authors.identifier: 0000-0003-2938-4063
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin , Belfield, Dublin 4, Ireland.
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Dawson
mesh_terms: D000327:Adsorption; D001665:Binding Sites; D002478:Cells, Cultured; D018604:Epitope Mapping; D057809:HEK293 Cells; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry; D008077:Lipoproteins, LDL / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D000066970:Protein Corona / Q000737:chemistry*; D017452:Receptors, IgG / Q000737:chemistry*; D011973:Receptors, LDL / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D007074:Immunoglobulin G; D008077:Lipoproteins, LDL; D000066970:Protein Corona; D017452:Receptors, IgG; D011973:Receptors, LDL
keywords: biomolecular corona; epitope; internalization; low-density lipoprotein; nanoparticle; receptor