title: Implantation of a Poly-L-Lactide GCSF-Functionalized Scaffold in a Model of Chronic Myocardial Infarction.
abstract: A previously developed poly-L-lactide scaffold releasing granulocyte colony-stimulating factor (PLLA/GCSF) was tested in a rabbit chronic model of myocardial infarction (MI) as a ventricular patch. Control groups were constituted by healthy, chronic MI and nonfunctionalized PLLA scaffold. PLLA-based electrospun scaffold efficiently integrated into a chronic infarcted myocardium. Functionalization of the biopolymer with GCSF led to increased fibroblast-like vimentin-positive cellular colonization and reduced inflammatory cell infiltration within the micrometric fiber mesh in comparison to nonfunctionalized scaffold; PLLA/GCSF polymer induced an angiogenetic process with a statistically significant increase in the number of neovessels compared to the nonfunctionalized scaffold; PLLA/GCSF implanted at the infarcted zone induced a reorganization of the ECM architecture leading to connective tissue deposition and scar remodeling. These findings were coupled with a reduction in end-systolic and end-diastolic volumes, indicating a preventive effect of the scaffold on ventricular dilation, and an improvement in cardiac performance.
authors.affiliation: Department of Cardiothoracic Surgery, Golden Jubilee National Hospital, Agamemnon Street Clydebank, Glasgow, G81 4DY, UK. cristianospadaccio@gmail.com.|Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK. cristianospadaccio@gmail.com.
authors.forename: Cristiano
authors.initials: C
authors.lastname: Spadaccio
authors.affiliation: Department of Cardiac Surgery, Centre Cardiologique du Nord de Saint-Denis, Paris, France.
authors.forename: Francesco
authors.initials: F
authors.lastname: Nappi
authors.affiliation: Laboratory of Virology, Regina Elena Institute for Cancer Research, Rome, Italy.
authors.forename: Federico
authors.initials: F
authors.lastname: De Marco
authors.affiliation: Department of Imaging and Diagnostics, Università Campus Bio Medico di Roma, Rome, Italy.
authors.forename: Pietro
authors.initials: P
authors.lastname: Sedati
authors.affiliation: Department of Pathology, Università Campus Bio Medico di Roma, Rome, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Taffon
authors.affiliation: Department of Cardiac Surgery, Università Campus Bio Medico di Roma, Rome, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Nenna
authors.affiliation: Department of Pathology, Università Campus Bio Medico di Roma, Rome, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Crescenzi
authors.affiliation: Department of Cardiac Surgery, Università Campus Bio Medico di Roma, Rome, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Chello
authors.affiliation: Tissue Engineering Lab, Università Campus Bio Medico di Roma, Rome, Italy.
authors.forename: Marcella
authors.initials: M
authors.lastname: Trombetta
authors.affiliation: Department of Cardiac Surgery, Liverpool Heart and Chest Hospital, Liverpool, UK.
authors.forename: Ivancarmine
authors.initials: I
authors.lastname: Gambardella
authors.affiliation: Tissue Engineering Lab, Università Campus Bio Medico di Roma, Rome, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Rainer
mesh_terms: D000818:Animals; D002908:Chronic Disease; D000072226:Computed Tomography Angiography; D004195:Disease Models, Animal; D004337:Drug Carriers; D004339:Drug Compounding; D004452:Echocardiography; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D000069585:Filgrastim / Q000008:administration & dosage* / Q000737:chemistry; D009203:Myocardial Infarction / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D018919:Neovascularization, Physiologic / Q000187:drug effects; D011091:Polyesters / Q000737:chemistry*; D011817:Rabbits; D020127:Recovery of Function; D013997:Time Factors; D016277:Ventricular Function, Left / Q000187:drug effects; D020257:Ventricular Remodeling / Q000187:drug effects; D014746:Vimentin / Q000378:metabolism
chemical_list: D004337:Drug Carriers; D011091:Polyesters; D014746:Vimentin; C033616:poly(lactide); D000069585:Filgrastim
keywords: 3D scaffold; Cardiac graft; Electrospinning; GCSF; Myocardial infarction; PLLA; Tissue engineering