title: Conjugatable and Bioreduction Cleavable Linker for the 5'-Functionalization of Oligonucleotides.
abstract: An efficient conjugatable and bioreduction cleavable linker was designed and synthesized for the 5'-terminal ends of oligonucleotides. A phosphoramidite reagent bearing this linker was successfully applied to solid phase synthesis and incorporated at the 5'-terminal ends of oligonucleotides. The controlled pore glass (CPG)-supported oligonucleotides were subsequently conjugated to a diverse range of functional molecules using a CuAAC reaction. The synthesized oligonucleotide conjugates were then cleaved using a nitroreductase/NADH bioreduction system to release the naked oligonucleotides.
authors.affiliation: Department of Material and Life Chemistry, Faculty of Engineering, Kanagawa University , 3-27-1 Rokkakubashi, Kanagawa-ku, Yokohama 221-8686, Japan.
authors.forename: Hisao
authors.identifier: 0000-0003-4061-101X
authors.initials: H
authors.lastname: Saneyoshi
authors.affiliation: Department of Material and Life Chemistry, Faculty of Engineering, Kanagawa University , 3-27-1 Rokkakubashi, Kanagawa-ku, Yokohama 221-8686, Japan.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Yamamoto
authors.affiliation: Department of Material and Life Chemistry, Faculty of Engineering, Kanagawa University , 3-27-1 Rokkakubashi, Kanagawa-ku, Yokohama 221-8686, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Kondo
authors.affiliation: Department of Material and Life Chemistry, Faculty of Engineering, Kanagawa University , 3-27-1 Rokkakubashi, Kanagawa-ku, Yokohama 221-8686, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hiyoshi
authors.affiliation: Department of Material and Life Chemistry, Faculty of Engineering, Kanagawa University , 3-27-1 Rokkakubashi, Kanagawa-ku, Yokohama 221-8686, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Ono
mesh_terms: D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry*; D010084:Oxidation-Reduction
chemical_list: D009841:Oligonucleotides