title: MiR-508-5p is a prognostic marker and inhibits cell proliferation and migration in glioma.
abstract: Increasing evidence has informed that dysregulation of microRNAs (miRNAs) may contribute to carcinogenesis in human. The aim of the present study was to determine the role of miR-508-5p in glioma.
  
  
  
  Quantitative real-time PCR was performed to detect the expression levels of miR-508-5p in glioma and normal control tissues. In vitro, migration assays and a wound-healing assay were performed to determine the effects of miR-508-5p. Associations between miR-508-5p expressions and various clinicopathological characteristics were analyzed. Survival rate was determined with Kaplan-Meier. Univariate and multivariate analyses were performed to estimate the effects of the expression of miR-508-5p on survival.
  
  
  
  The expression of miR-508-5p was downregulated in glioma tissues and cell lines. Low miR-508-5p expression was related to WHO grade (p = 0.005) and KPS score (p = 0.013). Moreover, Kaplan-Meier survival analysis showed that low miR-508-5p expression was significantly associated with short overall survival (p = 0.0059). Furthermore, multivariate analysis revealed that miR-508-5p was an independent prognostic factor for the overall survival in glioma (p = 0.002). Finally, forced expression of miR-508-5p could inhibit glioma cell growth and metastasis in vitro.
  
  
  
  Taken together, our study suggested that miR-508-5p may be served as a novel prognostic factor and may lead to new treatment strategies for glioma.
authors.affiliation: Department of Neurosurgery, Linyi People's Hospital, Linyi, Shandong, China. nn05nn1983@year.com.
authors.forename: Y-H
authors.initials: YH
authors.lastname: Liu
authors.forename: B
authors.initials: B
authors.lastname: Li
authors.forename: F-G
authors.initials: FG
authors.lastname: Meng
authors.forename: L
authors.initials: L
authors.lastname: Qiu
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D005910:Glioma / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C571981:MIRN508 microRNA, human; D035683:MicroRNAs