title: Effect of the topical application of an ethanol extract of quince seeds on the development of atopic dermatitis-like symptoms in NC/Nga mice.
abstract: BACKGROUND
  Quince (Cydonia oblonga Miller) is a deciduous shrub belonging to the Rosaceae family. Quince seed extract has long been used as a cosmetic ingredient for its moisturizing effect. However, little is known about whether quince seed extract has therapeutic effects on keratinocyte-associated skin inflammation.
  
  
  METHODS
  In the present study, we investigated the effect of the topical application of ethanol extract of quince seeds (QSEtE) on atopic dermatitis (AD) symptoms in NC/Nga mice. The direct effect of QSEtE on keratinocytes was evaluated using the human keratinocyte cell line HaCaT.
  
  
  RESULTS
  The preliminary application of QSEtE markedly reduced house dust mite allergen-induced skin lesions. The expression of thymus- and activation-regulated chemokine (TARC) in dorsal skin was downregulated. QSEtE directly suppressed the expression and production of TARC in HaCaT cells.
  
  
  CONCLUSIONS
  The results suggest that the topical application of QSEtE is effective in preventing the onset of and ameliorating the atopic symptoms of keratinocyte-associated skin inflammation by suppressing TARC production in keratinocytes.
authors.affiliation: Department of Sciences of Functional Foods, Graduate School of Agriculture, Shinshu University, 8304 Minamiminowa, Kamiina, Nagano, 399-4598, Japan. tkawafb@shinshu-u.ac.jp.|Research Center for Fungal & Microbial Dynamism, Shinshu University, 8304 Minamiminowa, Kamiina, Nagano, 399-4598, Japan. tkawafb@shinshu-u.ac.jp.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kawahara
authors.affiliation: Department of Sciences of Functional Foods, Graduate School of Agriculture, Shinshu University, 8304 Minamiminowa, Kamiina, Nagano, 399-4598, Japan.
authors.forename: Kanako
authors.initials: K
authors.lastname: Tsutsui
authors.affiliation: Department of Sciences of Functional Foods, Graduate School of Agriculture, Shinshu University, 8304 Minamiminowa, Kamiina, Nagano, 399-4598, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Nakanishi
authors.affiliation: Jupon International, 4-31-5 Nishikojiya, Ota-ku, Tokyo, 144-0034, Japan.
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Inoue
authors.affiliation: Department of Sciences of Functional Foods, Graduate School of Agriculture, Shinshu University, 8304 Minamiminowa, Kamiina, Nagano, 399-4598, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Hamauzu
mesh_terms: D000287:Administration, Topical; D000818:Animals; D002460:Cell Line; D003876:Dermatitis, Atopic / Q000473:pathology / Q000517:prevention & control*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects; D051379:Mice; D010936:Plant Extracts / Q000627:therapeutic use*; D060888:Real-Time Polymerase Chain Reaction; D027824:Rosaceae / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D013047:Specific Pathogen-Free Organisms
chemical_list: D010936:Plant Extracts
keywords: Atopic dermatitis; Cydonia oblonga; Keratinocyte; Quince seed; Thymus- and activation-regulated Chemokine