title: Analysis of Longissimus thoracis Protein Expression Associated with Variation in Carcass Quality Grade and Marbling of Beef Cattle Raised in the Pacific Northwestern United States.
abstract: Longissimus thoracis (LD) samples from 500 cattle were screened for protein expression differences relative to carcass quality grade. The LD of the top 5% (low prime and high choice, HQ) and bottom 5% (low select, LQ) carcasses were analyzed using two-dimensional difference gel electrophoresis and Western blot. Following initial screening, 11 candidate proteins were selected for Western blot analyses. Differentially expressed proteins were clustered into four groups: (1) heat shock proteins and oxidative protection, (2) sarcomeric proteins (muscle maturity and fiber type), (3) metabolism and energetics, and (4) miscellaneous proteins. Proteins from groups 1 and 2 were greater in HQ carcasses. Alternatively, increased quantities of proteins from group 3 were observed in LQ carcasses. Proteomic differences provide insights into pathways contributing to carcass quality grade. A deeper understanding of the physiological pathways involved in carcass quality grade development may allow producers to employ production practices that improve quality grade.
authors.affiliation: Department of Animal and Veterinary Sciences. University of Idaho , 606 Rayburn Street Moscow, Idaho 83844-2330, United States.
authors.forename: Kara J
authors.identifier: http://orcid.org/0000-0002-7506-5293
authors.initials: KJ
authors.lastname: Thornton
authors.affiliation: Department of Animal and Veterinary Sciences. University of Idaho , 606 Rayburn Street Moscow, Idaho 83844-2330, United States.
authors.forename: Kalyan C
authors.initials: KC
authors.lastname: Chapalamadugu
authors.affiliation: Department of Animal and Veterinary Sciences. University of Idaho , 606 Rayburn Street Moscow, Idaho 83844-2330, United States.
authors.forename: Eric M
authors.initials: EM
authors.lastname: Eldredge
authors.affiliation: Department of Animal and Veterinary Sciences. University of Idaho , 606 Rayburn Street Moscow, Idaho 83844-2330, United States.
authors.forename: Gordon K
authors.initials: GK
authors.lastname: Murdoch
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism; D015205:Northwestern United States; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D057825:Two-Dimensional Difference Gel Electrophoresis
chemical_list: D011506:Proteins
keywords: bovine; carcass quality grade; heat shock proteins; proteolysis; proteome