title: miRNA-34c inhibits myoblasts proliferation by targeting YY1.
abstract: miRNAs are increasingly being implicated as key regulators of cell proliferation, apoptosis, and differentiation. miRNA-34c appears to play a crucial role in cancer pathogenesis wherein it exerts its effect as a tumor suppressor. However, the role of miR-34c in myoblast proliferation remains poorly understood. Here, we found that overexpression miR-34c inhibited myoblasts proliferation by reducing the protein and mRNA expression of cell cycle genes. In contrast, blocking the function of miR-34c promoted myoblasts proliferation and increased the protein and mRNA expression of cell cycle genes. Moreover, miR-34c directly targeted YY1 and inhibited its expression. Similar to overexpression miR-34c, knockdown of YY1 by siRNA suppressed myoblasts proliferation. Our study provides novel evidence for a role of miR-34c in inhibiting myoblasts proliferation by repressing YY1. Thus, miR-34c has the potential to be used to enhance skeletal muscle development and regeneration.
authors.affiliation: a The State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University , Beijing , China.
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.affiliation: a The State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University , Beijing , China.
authors.forename: Chuncheng
authors.initials: C
authors.lastname: Liu
authors.affiliation: a The State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University , Beijing , China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Su
authors.affiliation: a The State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University , Beijing , China.
authors.forename: Kuo
authors.initials: K
authors.lastname: Zhang
authors.affiliation: a The State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University , Beijing , China.
authors.forename: Yuying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: a The State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University , Beijing , China.
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.affiliation: a The State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University , Beijing , China.
authors.forename: Mengxu
authors.initials: M
authors.lastname: Ge
authors.affiliation: a The State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University , Beijing , China.
authors.forename: Lijie
authors.initials: L
authors.lastname: Gu
authors.affiliation: a The State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University , Beijing , China.
authors.forename: Tianyu
authors.initials: T
authors.lastname: Lu
authors.affiliation: a The State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University , Beijing , China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: a The State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University , Beijing , China.
authors.forename: Zhengquan
authors.initials: Z
authors.lastname: Yu
authors.affiliation: a The State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University , Beijing , China.
authors.forename: Qingyong
authors.initials: Q
authors.lastname: Meng
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism; D032446:Myoblasts / Q000166:cytology* / Q000378:metabolism*; D012038:Regeneration; D015854:Up-Regulation / Q000235:genetics; D050986:YY1 Transcription Factor / Q000378:metabolism*
chemical_list: C551848:MIRN34a microRNA, mouse; D035683:MicroRNAs; D050986:YY1 Transcription Factor
keywords: YY1; cell cycle; miR-34c; myoblast; proliferation