title: Genomics-Guided Exploitation of Lipopeptide Diversity in Myxobacteria.
abstract: Analysis of 122 myxobacterial genome sequences suggested 16 strains as producers of the myxochromide lipopeptide family. Detailed sequence comparison of the respective mch biosynthetic gene clusters informed a genome-mining approach, ultimately leading to the discovery and chemical characterization of four novel myxochromide core types. The myxochromide megasynthetase is subject to evolutionary diversification, resulting in considerable structural diversity of biosynthesis products. The observed differences are due to the number, type, sequence, and configuration of the incorporated amino acids. The analysis revealed molecular details on how point mutations and recombination events led to structural diversity. It also gave insights into the evolutionary scenarios that have led to the emergence of mch clusters in different strains and genera of myxobacteria.
authors.affiliation: Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research and Pharmaceutical Biotechnology at Saarland University, Saarland University Campus , Building E8.1, 66123 Saarbrücken, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Burgard
authors.affiliation: Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research and Pharmaceutical Biotechnology at Saarland University, Saarland University Campus , Building E8.1, 66123 Saarbrücken, Germany.
authors.forename: Nestor
authors.initials: N
authors.lastname: Zaburannyi
authors.affiliation: Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research and Pharmaceutical Biotechnology at Saarland University, Saarland University Campus , Building E8.1, 66123 Saarbrücken, Germany.
authors.forename: Suvd
authors.initials: S
authors.lastname: Nadmid
authors.affiliation: IStLS - Information Services to Life Sciences , Härlestraße 24/1, 78727 Oberndorf am Neckar/Boll, Germany.
authors.forename: Josef
authors.initials: J
authors.lastname: Maier
authors.affiliation: Microbiology and Biochemistry of Secondary Metabolites Unit, Okinawa Institute of Science and Technology (OIST) , 1919-1 Tancha, Onna-son, Okinawa 904-0495, Japan.
authors.forename: Holger
authors.initials: H
authors.lastname: Jenke-Kodama
authors.affiliation: Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research and Pharmaceutical Biotechnology at Saarland University, Saarland University Campus , Building E8.1, 66123 Saarbrücken, Germany.
authors.forename: Eva
authors.initials: E
authors.lastname: Luxenburger
authors.affiliation: ATG Biosynthetics GmbH , Weberstraße 40, 79249 Merzhausen, Germany.
authors.forename: Hubert S
authors.initials: HS
authors.lastname: Bernauer
authors.affiliation: Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research and Pharmaceutical Biotechnology at Saarland University, Saarland University Campus , Building E8.1, 66123 Saarbrücken, Germany.
authors.forename: Silke C
authors.identifier: 0000-0002-3626-795X
authors.initials: SC
authors.lastname: Wenzel
mesh_terms: D023281:Genomics; D055666:Lipopeptides / Q000378:metabolism*; D005810:Multigene Family; D009231:Myxococcales / Q000235:genetics* / Q000378:metabolism
chemical_list: D055666:Lipopeptides