title: Measurement of Angiotensin Peptides: HPLC-RIA.
abstract: The renin-angiotensin system (RAS) is a complex circulating and tissue-based system. There are multiple pathways for the formation and degradation of peptides. In order to understand the functions of the system, characterization of angiotensin peptides (products and substrates) is important. Radioimmunoassays with the requisite specificity and sensitivity have been developed to allow for the characterization and quantification of circulating and tissue angiotensins. Here, we describe the appropriate methods for collecting the tissue and blood, the extractions steps required to partially purify and remove larger molecular weight-interfering proteins from tissue and plasma, and the radioimmunoassay of three of the peptides of this system (Ang I, Ang II, and Ang-(1-7)), as well as the verification of immunoreactive identity for Ang II and Ang-(1-7) by combined high-performance liquid chromatography-RIA analysis.
authors.affiliation: Department of Surgery, Hypertension & Vascular Research, Cardiovascular Sciences Center, Wake Forest University School of Medicine, Medical Center Blvd, Winston-Salem, NC, 27157-1032, USA. bbrosnih@wakehealth.edu.
authors.forename: K Bridget
authors.initials: KB
authors.lastname: Brosnihan
authors.affiliation: Department of Surgery, Hypertension & Vascular Research, Cardiovascular Sciences Center, Wake Forest University School of Medicine, Medical Center Blvd, Winston-Salem, NC, 27157-1032, USA.
authors.forename: Mark C
authors.initials: MC
authors.lastname: Chappell
mesh_terms: D000803:Angiotensin I / Q000032:analysis / Q000097:blood*; D000804:Angiotensin II / Q000032:analysis / Q000097:blood*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis / Q000097:blood*; D011863:Radioimmunoassay / Q000379:methods*; D012084:Renin-Angiotensin System / Q000502:physiology
chemical_list: D010446:Peptide Fragments; D000804:Angiotensin II; D000803:Angiotensin I; C118790:angiotensin I (1-7)
keywords: Angiotensin I; Angiotensin II; Angiotensin-(1-7); Extraction; HPLC; Homogenization; Radioimmunoassay