title: Evaluation of Genotoxic and Mutagenic Activity of Organic Extracts from Drinking Water Sources.
abstract: An increasing number of industrial, agricultural and commercial chemicals in the aquatic environment lead to various deleterious effects on organisms, which is becoming a serious global health concern. In this study, the Ames test and SOS/umu test were conducted to investigate the potential genotoxicity and mutagenicity caused by organic extracts from drinking water sources. Organic content of source water was extracted with XAD-2 resin column and organic solvents. Four doses of the extract equivalent to 0.25, 0.5, 1 and 2L of source water were tested for toxicity. All the water samples were collected from six different locations in Guangdong province. The results of the Ames test and SOS/umu test showed that all the organic extracts from the water samples could induce different levels of DNA damage and mutagenic potentials at the dose of 2 L in the absence of S9 mix, which demonstrated the existence of genotoxicity and mutagenicity. Additionally, we found that Salmonella typhimurium strain TA98 was more sensitive for the mutagen. Correlation analysis between genotoxicity, Organochlorine Pesticides (OCPs) and Polycyclic Aromatic Hydrocarbons (PAHs) showed that most individual OCPs were frame shift toxicants in drinking water sources, and there was no correlation with total OCPs and PAHs.
authors.affiliation: Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Guan
authors.affiliation: Jiangxi Academy of Forestry, Nanchang, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Croucher Institute for Environmental Sciences, and Department of Biology, Hong Kong Baptist University, Hong Kong China.
authors.forename: Minghung
authors.initials: M
authors.lastname: Wong
authors.affiliation: Guangdong Institute of Microbiology, Guangdong Open Laboratory of Applied Microbiology, and Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou, China.
authors.forename: Guoping
authors.initials: G
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Organic Geochemist, Protection, Guangzhou Institute of Geochemistry, Chinese Academy of Science, Guangzhou, China.
authors.forename: Taicheng
authors.initials: T
authors.lastname: An
authors.affiliation: Guangdong Institute of Microbiology, Guangdong Open Laboratory of Applied Microbiology, and Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Guo
authors.affiliation: Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China.
authors.forename: Guoxia
authors.identifier: http://orcid.org/0000-0003-2530-3238
authors.initials: G
authors.lastname: Zhang
mesh_terms: D002681:China; D060766:Drinking Water / Q000737:chemistry*; D016296:Mutagenesis; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis*; D010575:Pesticides / Q000032:analysis*; D012486:Salmonella typhimurium; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D060766:Drinking Water; D009153:Mutagens; D010575:Pesticides; D014874:Water Pollutants, Chemical