title: Influence of carrageenan on cytokine production and cellular activity of mouse peritoneal macrophages and its effect on experimental endotoxemia.
abstract: The in vivo effect of κ/β-carrageenan isolated from the red alga Tichocarpus crinitus on cytokine synthesis and cellular activity of murine peritoneal macrophages and also the protective effect of polysaccharides in LPS-induced endotoxemia in mice was studied. It was established that κ/β-carrageenan given orally at a dose of 100 mg/kg stimulates the induction of anti-inflammatory cytokines (IL-10) in mouse blood cells by more than 2.5-fold compared with control, with no effect on pro-inflammatory cytokine (TNF-α) production. Pretreating mice with carrageenan once a day before injecting LPS increased the levels of IL-10 by 2.5-fold and reduced TNF-α production by 2-fold compared with control. So, κ/β-carrageenan alone and in combination with LPS enhanced the cellular activity and mobility of peritoneal macrophages by increasing cell adhesion and migration compared with control. LPS activated cells intensively, sometimes resulting in their destruction by necrosis; carrageenan pretreatment reduced the excessive inflammatory cell activation caused by LPS. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1549-1557, 2017.
authors.affiliation: School of Biomedicine, School of Natural Sciences Far Eastern Federal University, 8 Sukhanova Street, Vladivostok, 690950.
authors.forename: A A
authors.initials: AA
authors.lastname: Kalitnik
authors.affiliation: School of Biomedicine, School of Natural Sciences Far Eastern Federal University, 8 Sukhanova Street, Vladivostok, 690950.|A. V. Zhirmunsky Institute of Marine Biology, FEB RAS, 17 Palchevskogo street, Vladivostok, 690022.
authors.forename: Y A
authors.initials: YA
authors.lastname: Karetin
authors.affiliation: G. B. Elyakov Pacific Institute of Bioorganic Chemistry, FEB RAS, 100-let Vladivostoku Prospect, Vladivostok, 690022.
authors.forename: A O
authors.initials: AO
authors.lastname: Kravchenko
authors.affiliation: A. V. Zhirmunsky Institute of Marine Biology, FEB RAS, 17 Palchevskogo street, Vladivostok, 690022.
authors.forename: E I
authors.initials: EI
authors.lastname: Khasina
authors.affiliation: G. B. Elyakov Pacific Institute of Bioorganic Chemistry, FEB RAS, 100-let Vladivostoku Prospect, Vladivostok, 690022.
authors.forename: I M
authors.initials: IM
authors.lastname: Yermak
mesh_terms: D000818:Animals; D002351:Carrageenan / Q000737:chemistry / Q000494:pharmacology; D004195:Disease Models, Animal; D019446:Endotoxemia / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D005260:Female; D016753:Interleukin-10 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000633:toxicity*; D017737:Macrophages, Peritoneal / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008808:Mice, Inbred CBA; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: C556062:IL10 protein, mouse; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D002351:Carrageenan
keywords: LPS-induced endotoxemia; cellular activity; cytokines; murine peritoneal macrophages; κ/β-carrageenan