title: Mechanotransductive cascade of Myo-II-dependent mesoderm and endoderm invaginations in embryo gastrulation.
abstract: Animal development consists of a cascade of tissue differentiation and shape change. Associated mechanical signals regulate tissue differentiation. Here we demonstrate that endogenous mechanical cues also trigger biochemical pathways, generating the active morphogenetic movements shaping animal development through a mechanotransductive cascade of Myo-II medio-apical stabilization. To mimic physiological tissue deformation with a cell scale resolution, liposomes containing magnetic nanoparticles are injected into embryonic epithelia and submitted to time-variable forces generated by a linear array of micrometric soft magnets. Periodic magnetically induced deformations quantitatively phenocopy the soft mechanical endogenous snail-dependent apex pulsations, rescue the medio-apical accumulation of Rok, Myo-II and subsequent mesoderm invagination lacking in sna mutants, in a Fog-dependent mechanotransductive process. Mesoderm invagination then activates Myo-II apical accumulation, in a similar Fog-dependent mechanotransductive process, which in turn initiates endoderm invagination. This reveals the existence of a highly dynamic self-inductive cascade of mesoderm and endoderm invaginations, regulated by mechano-induced medio-apical stabilization of Myo-II.
authors.affiliation: Mechanics and Genetics of Embryonic Development group, Institut Curie, PSL Research University, CNRS, UMR168, Inserm, Marie Curie Univ Paris 06, Institut Curie, 11 rue Pierre et Marie Curie, 75005 Paris, France.
authors.forename: Démosthène
authors.initials: D
authors.lastname: Mitrossilis
authors.affiliation: Mechanics and Genetics of Embryonic Development group, Institut Curie, PSL Research University, CNRS, UMR168, Inserm, Marie Curie Univ Paris 06, Institut Curie, 11 rue Pierre et Marie Curie, 75005 Paris, France.
authors.forename: Jens-Christian
authors.initials: JC
authors.lastname: Röper
authors.affiliation: Univ. Grenoble Alpes, Inst NEEL, F-38042 Grenoble, France.|CNRS, Inst NEEL, F-38042 Grenoble, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Le Roy
authors.affiliation: Mechanics and Genetics of Embryonic Development group, Institut Curie, PSL Research University, CNRS, UMR168, Inserm, Marie Curie Univ Paris 06, Institut Curie, 11 rue Pierre et Marie Curie, 75005 Paris, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Driquez
authors.affiliation: Sorbonne Universités, Université Pierre et Marie Curie Univ Paris 06, Laboratoire PHENIX-UMR 8234, 1 Place Jussieu, 75005 Paris, France.
authors.forename: Aude
authors.initials: A
authors.lastname: Michel
authors.affiliation: Sorbonne Universités, Université Pierre et Marie Curie Univ Paris 06, Laboratoire PHENIX-UMR 8234, 1 Place Jussieu, 75005 Paris, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Ménager
authors.affiliation: Univ. Grenoble Alpes, Inst NEEL, F-38042 Grenoble, France.|CNRS, Inst NEEL, F-38042 Grenoble, France.
authors.forename: Gorky
authors.initials: G
authors.lastname: Shaw
authors.affiliation: Univ. Grenoble Alpes, Inst NEEL, F-38042 Grenoble, France.|CNRS, Inst NEEL, F-38042 Grenoble, France.
authors.forename: Simon
authors.initials: S
authors.lastname: Le Denmat
authors.affiliation: Univ. Grenoble Alpes, Inst NEEL, F-38042 Grenoble, France.|CNRS, Inst NEEL, F-38042 Grenoble, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Ranno
authors.affiliation: Univ. Grenoble Alpes, Inst NEEL, F-38042 Grenoble, France.|CNRS, Inst NEEL, F-38042 Grenoble, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Dumas-Bouchiat
authors.affiliation: Univ. Grenoble Alpes, Inst NEEL, F-38042 Grenoble, France.|CNRS, Inst NEEL, F-38042 Grenoble, France.
authors.forename: Nora M
authors.initials: NM
authors.lastname: Dempsey
authors.affiliation: Mechanics and Genetics of Embryonic Development group, Institut Curie, PSL Research University, CNRS, UMR168, Inserm, Marie Curie Univ Paris 06, Institut Curie, 11 rue Pierre et Marie Curie, 75005 Paris, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Farge
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000196:embryology*; D004625:Embryo, Nonmammalian / Q000502:physiology*; D004707:Endoderm / Q000502:physiology*; D054262:Gastrulation / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D008280:Magnetics; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D008648:Mesoderm / Q000502:physiology*; D024462:Myosin Type II / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference
chemical_list: D024462:Myosin Type II