title: Photomediated Larvicidal Activity of Pheophorbide a against Cercaria Larvae ofFasciola gigantica.
abstract: Fasciolosis is a parasitic disease caused byFasciola gigantica. The freshwater snailLymnaea acuminatais the intermediate host ofF. giganticawhich cause endemic fasciolosis in the northern part of India. To investigate larvicidal activity of pure and laboratory extracted pheophorbide a (Pa) against cercaria larvae ofF. gigantica, data were analyzed in different spectra of visible light, sunlight, and laboratory conditions. Photostimulation of chlorophyll derivative pheophorbide a (Pa) caused time and concentration dependent larvicidal activity against cercaria larvae ofF. gigantica. Larvicidal activity of pure Pa under 650 nm and 400-650 nm (8 h LC500.006 mg/10 mL) was more pronounced than extracted Pa under same irradiations (650 nm LC500.12 mg/10 mL, 400-650 nm LC500.14 mg/10 mL). Lowest toxicity of pure (8 h LC500.14 mg/10 mL) and extracted Pa (8 h LC501.25 mg/10 mL) was noted under 400 nm. Pa was found to be toxic in laboratory conditions also. The results presented in this paper indicate that pheophorbide a possess potential larvicidal activity againstFasciola giganticalarvae in different wavelengths of visible light, sunlight, and laboratory conditions.
authors.affiliation: Malacology Laboratory, Department of Zoology, D.D.U. Gorakhpur University, Gorakhpur, Uttar Pradesh 273 009, India.
authors.forename: Divya Jyoti
authors.initials: DJ
authors.lastname: Singh
authors.affiliation: Malacology Laboratory, Department of Zoology, D.D.U. Gorakhpur University, Gorakhpur, Uttar Pradesh 273 009, India.
authors.forename: Vinay Kumar
authors.initials: VK
authors.lastname: Singh
authors.affiliation: Malacology Laboratory, Department of Zoology, D.D.U. Gorakhpur University, Gorakhpur, Uttar Pradesh 273 009, India.
authors.forename: D K
authors.identifier: 0000-0003-3701-7749
authors.initials: DK
authors.lastname: Singh