title: Genetic Structure and Drug Susceptibility Patterns ofMycobacterium tuberculosisComplex Strains Responsible of Human Pulmonary Tuberculosis in the Major Rearing Region in Cameroon.
abstract: Background. Cameroon this last decade continues to present a low contribution ofM. africanumandM. bovisin human tuberculosis (TB), whileM. boviswas prevalent in cattle but all these pieces of information only concerned West and Center regions.Methods. We carried out the first study in Adamaoua, one of the most rearing regions of Cameroon, on the genetic structure and drug susceptibility of the MTBC strains isolated from newly diagnosed sputum smear-positive patients aged 15 years and above. For that purpose, spoligotyping, a modified 15 standard MIRU/VNTR loci typing, and the proportion method were used.Results. Four hundred and thirty-seven MTBC isolates were analyzed by spoligotyping. Of these, 423 were identified asM. tuberculosis, within the Cameroon family being dominant with 278 (65.7%) isolates; twelve (2.75%) isolates were classified asM. africanumand two asM. bovis. MIRU/VNTR typing of the most prevalent sublineage (SIT 61) suggested that this lineage is not a unique clone as thought earlier but could constitute a group of strains implicated to different pocket of TB transmission. OnlyM. tuberculosissublineages were associated with antituberculosis drug resistance.Conclusion. These results showed the weak contribution ofM. africanumandM. bovisto human active pulmonary tuberculosis in Cameroon even in the rearing region.
authors.affiliation: Mycobacteriology Service, Reference Laboratory of NTP, Centre Pasteur of Cameroon, Cameroon-Pasteur Institute International Network, Yaoundé, Cameroon; Department of Biochemistry, Faculty of Sciences, University of Douala, Yaoundé, Cameroon.
authors.forename: Francioli
authors.identifier: 0000-0002-7241-6801
authors.initials: F
authors.lastname: Koro Koro
authors.affiliation: FAIRMED Foundation, Yaoundé, Cameroon.
authors.forename: Alphonse
authors.initials: A
authors.lastname: Um Boock
authors.affiliation: Mycobacteriology Service, Reference Laboratory of NTP, Centre Pasteur of Cameroon, Cameroon-Pasteur Institute International Network, Yaoundé, Cameroon.
authors.forename: Afi Leslie
authors.identifier: 0000-0002-1591-0942
authors.initials: AL
authors.lastname: Kaiyven
authors.affiliation: German International Cooperation (GIZ), Yaoundé, Cameroon.
authors.forename: Juergen
authors.initials: J
authors.lastname: Noeske
authors.affiliation: Caribbean Public Health Agency, Port of Spain, Trinidad and Tobago.
authors.forename: Cristina
authors.initials: C
authors.lastname: Gutierrez
authors.affiliation: Faculty of Medicine and Biomedical Sciences/Jamot Hospital, University of Bamenda, Yaoundé, Cameroon.
authors.forename: Christopher
authors.initials: C
authors.lastname: Kuaban
authors.affiliation: Department of Microbiology, Faculty of Sciences, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: François-Xavier
authors.initials: FX
authors.lastname: Etoa
authors.affiliation: Mycobacteriology Service, Reference Laboratory of NTP, Centre Pasteur of Cameroon, Cameroon-Pasteur Institute International Network, Yaoundé, Cameroon.
authors.forename: Sara Irène
authors.initials: SI
authors.lastname: Eyangoh
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000995:Antitubercular Agents / Q000627:therapeutic use; D002163:Cameroon; D002417:Cattle; D003430:Cross-Sectional Studies; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000254:growth & development*; D013183:Sputum / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000188:drug therapy / Q000382:microbiology*; D055815:Young Adult
chemical_list: D000995:Antitubercular Agents