title: Predictors for successful PBSC collection on the fourth day of G-CSF-induced mobilization in allogeneic stem cell donors.
abstract: Peripheral blood stem cells (PBSCs) used for allogeneic transplantation are collected by apheresis after pre-treatment of donors with G-CSF. Using modern apheresis devices stem cells can be collected more efficiently. It was studied whether collection on the 4th instead of the 5th day after initiation of G-CSF treatment might be feasible. Stem cell yields that could have been collected on day 4 were calculated in two cohorts treated with 10 µg/kg G-CSF once daily (n = 106, cohort I) or 5 µg/kg twice daily schedule (n = 85, cohort II). Harvests were predicted using the median collection efficiency (CE) of the apheresis machine and regarded successful when > 5.0 x106CD34+/kg recipient body weight. Successful harvests at day 4 could have been obtained in only 22.6% and 41.2% of donors in cohort I and II respectively, while the expected successful collections on day 5 were 55.7% and 76.5%. Individual donor factors that correlated with a successful harvest on day 4 were weight, BMI, age, ratio donor/recipient weight and total G-CSF dose in cohort I, whereas ratio donor/recipient weight was the only significant predictor in cohort II. Donor weight, BMI and total G-CSF dose correlated positively with CD34+values in the blood on day 4 in all donors. However, donor characteristics were not able to be used as strong predictors in daily practice. In conclusion, PBSC collection on day 4 will not result in a successful harvest in most stem cell donors, however using a twice daily G-CSF scheme increases the yield.
authors.affiliation: Department of Immunohematology and Blood Transfusion, Leiden University Medical Centre, Leiden, The Netherlands.
authors.forename: Anja
authors.initials: A
authors.lastname: van Oostrum
authors.affiliation: Department of Immunohematology and Blood Transfusion, Leiden University Medical Centre, Leiden, The Netherlands.|Centre for Clinical Transfusion Research, Sanquin, Leiden, The Netherlands.
authors.forename: Jaap Jan
authors.initials: JJ
authors.lastname: Zwaginga
authors.affiliation: Department of Hematology, Radboud University Medical Centre, Nijmegen, The Netherlands.
authors.forename: Sandra
authors.initials: S
authors.lastname: Croockewit
authors.affiliation: Department of Immunohematology and Blood Transfusion, Leiden University Medical Centre, Leiden, The Netherlands.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Overdevest
authors.affiliation: Matchis Foundation, Leiden, The Netherlands.
authors.forename: Mirjam
authors.initials: M
authors.lastname: Fechter
authors.affiliation: Department of Hematology, Radboud University Medical Centre, Nijmegen, The Netherlands.
authors.forename: Bart
authors.initials: B
authors.lastname: Ruiterkamp
authors.affiliation: Matchis Foundation, Leiden, The Netherlands.
authors.forename: Anneke
authors.initials: A
authors.lastname: Brand
authors.affiliation: Department of Immunohematology and Blood Transfusion, Leiden University Medical Centre, Leiden, The Netherlands.
authors.forename: Tanja
authors.initials: T
authors.lastname: Netelenbos
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000032:analysis; D001782:Blood Donors; D015992:Body Mass Index; D001835:Body Weight; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage* / Q000494:pharmacology; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods* / Q000592:standards; D006801:Humans; D008297:Male; D008875:Middle Aged; D000072916:Peripheral Blood Stem Cells / Q000166:cytology*; D013997:Time Factors; D014184:Transplantation, Homologous; D055815:Young Adult
chemical_list: D018952:Antigens, CD34; D016179:Granulocyte Colony-Stimulating Factor
keywords: G-CSF dosage; apheresis; mobilization; mononuclear cell collection