title: Responses of plasma glucose and nonesterified fatty acids to intravenous insulin tolerance tests in dairy cows during a 670-day lactation.
abstract: The metabolic response of dairy cows undergoing an extended lactation to an insulin tolerance test (ITT) was investigated. Twelve multiparous Holstein-Friesian cows that calved in late winter in a pasture-based system were managed for a 670-d lactation by delaying rebreeding. Four 5-wk experimental periods commenced at approximately 73, 217, 422, and 520 d in milk (DIM). Cows were offered a diet of perennial ryegrass (73 and 422 DIM) or pasture hay and silage (217 and 520 DIM) supplemented with 1 kg dry matter (DM) of grain (control; CON) or 6 kg DM of grain (GRN). Daily energy intake was approximately 160 and 215 MJ of metabolizable energy/cow for CON and GRN, respectively. At all other times, cows were managed as a single herd and grazed pasture supplemented with grain to an estimated daily intake of 180 MJ of metabolizable energy/cow. Cows were fitted with a jugular catheter during the final week of each experimental period. An ITT using 0.12 IU of insulin/kg of body weight (BW) was conducted on each cow at approximately 100, 250, 460, and 560 DIM. Cows in the GRN treatment had greater milk yield, milk solids yield, and BW than cows in the CON treatment. Within treatment, individual cow responses to the ITT were highly variable. Plasma glucose and nonesterified fatty acid (NEFA) concentrations declined at all stages of lactation. The clearance rate of plasma glucose was slower before 300 DIM than after 300 DIM, which indicates greater inhibition of hepatic glucose synthesis and uptake of glucose by insulin-dependent tissues later in the lactation. The clearance rate, area under the curve, and recovery of plasma NEFA were greatest at 100 DIM, indicating greater responsiveness to the antilipolytic effect of insulin in early lactation, but also greater lipolytic responsiveness. The variation in response to the ITT was mostly a result of DIM rather than diet. However, the plasma NEFA response showed interactions between diet and DIM, indicating that energy intake may affect tissue responses to insulin. The responsiveness of peripheral tissues to insulin, primarily adipose tissue, changed throughout a 670-d lactation and contributed to a greater proportion of nutrients being partitioned to body reserves at the expense of milk yield as lactation progressed. Both stage of lactation and dietary intake have a role in the determination of whole-body and peripheral tissue responses to insulin; however, the exact mechanisms in control of this are unclear.
authors.affiliation: Agriculture Victoria, Department of Economic Development, Jobs, Transport and Resources, Ellinbank, Victoria 3821, Australia. Electronic address: leah.marett@ecodev.vic.gov.au.
authors.forename: L C
authors.initials: LC
authors.lastname: Marett
authors.affiliation: Agriculture Victoria, Department of Economic Development, Jobs, Transport and Resources, Ellinbank, Victoria 3821, Australia.
authors.forename: M J
authors.initials: MJ
authors.lastname: Auldist
authors.affiliation: Agriculture Victoria, Department of Economic Development, Jobs, Transport and Resources, Ellinbank, Victoria 3821, Australia.
authors.forename: W J
authors.initials: WJ
authors.lastname: Wales
authors.affiliation: Faculty of Veterinary and Agricultural Science, The University of Melbourne, Werribee, Victoria 3030, Australia.
authors.forename: K L
authors.initials: KL
authors.lastname: Macmillan
authors.affiliation: Faculty of Veterinary and Agricultural Science, The University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: F R
authors.initials: FR
authors.lastname: Dunshea
authors.affiliation: Faculty of Veterinary and Agricultural Science, The University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: B J
authors.initials: BJ
authors.lastname: Leury
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D002417:Cattle; D004032:Diet / Q000662:veterinary; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D005260:Female; D007328:Insulin / Q000097:blood; D007774:Lactation; D008892:Milk / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D007328:Insulin
keywords: extended lactation; fatty acid metabolism; insulin sensitivity; nutrient partitioning