title: Bioremediation of PAH-contamined soils: Consequences on formation and degradation of polar-polycyclic aromatic compounds and microbial community abundance.
abstract: A bioslurry batch experiment was carried out over five months on three polycyclic aromatic compound (PAC) contaminated soils to study the PAC (PAH and polar-PAC) behavior during soil incubation and to evaluate the impact of PAC contamination on the abundance of microbial communities and functional PAH-degrading populations. Organic matter characteristics and reactivity, assessed through solvent extractable organic matter and PAC contents, and soil organic matter mineralization were monitored during 5 months. Total bacteria and fungi, and PAH-ring hydroxylating dioxygenase genes were quantified. Results showed that PAHs and polar-PACs were degraded with different degradation dynamics. Differences in degradation rates were observed among the three soils depending on PAH distribution and availability. Overall, low molecular weight compounds were preferentially degraded. Degradation selectivity between isomers and structurally similar compounds was observed which could be used to check the efficiency of bioremediation processes. Bacterial communities were dominant over fungi and were most likely responsible for PAC degradation. Abundance of PAH-degrading bacteria increased during incubations, but their proportion in the bacterial communities tended to decrease. The accumulation of some oxygenated-PACs during the bioslurry experiment underlines the necessity to monitor these compounds during application of remediation treatment on PAH contaminated soils.
authors.affiliation: Université de Lorraine, LIEC, UMR7360, Vandœuvre-lès-Nancy 54506, France; CNRS, LIEC, UMR7360, Vandœuvre-lès-Nancy 54506, France. Electronic address: coralie.biache@univ-lorraine.fr.
authors.forename: Coralie
authors.initials: C
authors.lastname: Biache
authors.affiliation: Université de Lorraine, LIEC, UMR7360, Vandœuvre-lès-Nancy 54506, France; CNRS, LIEC, UMR7360, Vandœuvre-lès-Nancy 54506, France.
authors.forename: Salma
authors.initials: S
authors.lastname: Ouali
authors.affiliation: Université de Lorraine, LIEC, UMR7360, Vandœuvre-lès-Nancy 54506, France; CNRS, LIEC, UMR7360, Vandœuvre-lès-Nancy 54506, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Cébron
authors.affiliation: Université de Lorraine, CNRS, CREGU, GeoRessources lab, UMR7359, Vandœuvre-lès-Nancy 54506, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Lorgeoux
authors.affiliation: BRGM, 3 avenue Claude Guillemin, BP 36009, Orléans Cedex 2 45060, France.
authors.forename: Stéfan
authors.initials: S
authors.lastname: Colombano
authors.affiliation: Université de Lorraine, LIEC, UMR7360, Vandœuvre-lès-Nancy 54506, France; CNRS, LIEC, UMR7360, Vandœuvre-lès-Nancy 54506, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Faure
mesh_terms: D001673:Biodegradation, Environmental; D007220:Industrial Waste; D015999:Multivariate Analysis; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D007220:Industrial Waste; D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants
keywords: Availability; Bacteria; Bioslurry; Fungi; PAH-degrader; Polycyclic aromatic hydrocarbon