title: Lymph node and circulating T cell characteristics are strongly correlated in end-stage renal disease patients, but highly differentiated T cells reside within the circulation.
abstract: Ageing is associated with changes in the peripheral T cell immune system, which can be influenced significantly by latent cytomegalovirus (CMV) infection. To what extent changes in circulating T cell populations correlate with T cell composition of the lymph node (LN) is unclear, but is crucial for a comprehensive understanding of the T cell system. T cells from peripheral blood (PB) and LN of end-stage renal disease patients were analysed for frequency of recent thymic emigrants using CD31 expression and T cell receptor excision circle content, relative telomere length and expression of differentiation markers. Compared with PB, LN contained relatively more CD4+than CD8+T cells (P < 0·001). The percentage of naive and central memory CD4+and CD8+T cells and thymic output parameters showed a strong linear correlation between PB and LN. Highly differentiated CD28nullT cells, being CD27-, CD57+or programmed death 1 (PD-1+), were found almost exclusively in the circulation but not in LN. An age-related decline in naive CD4+and CD8+T cell frequency was observed (P = 0·035 and P = 0·002, respectively) within LN, concomitant with an increase in central memory CD8+T cells (P = 0·033). Latent CMV infection increased dramatically the frequency of circulating terminally differentiated T cells, but did not alter T cell composition and ageing parameters of LN significantly. Overall T cell composition and measures of thymic function in PB and LN are correlated strongly. However, highly differentiated CD28nullT cells, which may comprise a large part of circulating T cells in CMV-seropositive individuals, are found almost exclusively within the circulation.
authors.affiliation: Department of Internal Medicine, section Nephrology and Transplantation, Erasmus University Medical Center, Rotterdam, the Netherlands.
authors.forename: B
authors.initials: B
authors.lastname: Dedeoglu
authors.affiliation: Department of Internal Medicine, section Nephrology and Transplantation, Erasmus University Medical Center, Rotterdam, the Netherlands.
authors.forename: A E
authors.initials: AE
authors.lastname: de Weerd
authors.affiliation: Department of Internal Medicine, section Nephrology and Transplantation, Erasmus University Medical Center, Rotterdam, the Netherlands.
authors.forename: L
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Immunology, Erasmus University Medical Center, Rotterdam, the Netherlands.
authors.forename: A W
authors.initials: AW
authors.lastname: Langerak
authors.affiliation: Department of Surgery, Erasmus University Medical Center, Rotterdam, the Netherlands.
authors.forename: F J
authors.initials: FJ
authors.lastname: Dor
authors.affiliation: Department of Internal Medicine, section Nephrology and Transplantation, Erasmus University Medical Center, Rotterdam, the Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: Klepper
authors.affiliation: Department of Internal Medicine, section Nephrology and Transplantation, Erasmus University Medical Center, Rotterdam, the Netherlands.
authors.forename: W
authors.initials: W
authors.lastname: Verschoor
authors.affiliation: Department of Internal Medicine, section Nephrology and Transplantation, Erasmus University Medical Center, Rotterdam, the Netherlands.
authors.forename: D
authors.initials: D
authors.lastname: Reijerkerk
authors.affiliation: Department of Internal Medicine, section Nephrology and Transplantation, Erasmus University Medical Center, Rotterdam, the Netherlands.
authors.forename: C C
authors.initials: CC
authors.lastname: Baan
authors.affiliation: Department of Internal Medicine, section Nephrology and Transplantation, Erasmus University Medical Center, Rotterdam, the Netherlands.
authors.forename: N H R
authors.initials: NH
authors.lastname: Litjens
authors.affiliation: Department of Internal Medicine, section Nephrology and Transplantation, Erasmus University Medical Center, Rotterdam, the Netherlands.
authors.forename: M G H
authors.initials: MG
authors.lastname: Betjes
mesh_terms: D000368:Aged; D000375:Aging / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation; D003586:Cytomegalovirus Infections / Q000276:immunology; D005260:Female; D006801:Humans; D007156:Immunologic Memory; D007676:Kidney Failure, Chronic / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology*; D008213:Lymphocyte Activation; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000235:genetics; D017735:Virus Latency
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D019408:Platelet Endothelial Cell Adhesion Molecule-1
keywords: T cells; ageing; end-stage renal disease; lymph node; peripheral blood