title: [The influence of catecholamines on Th17-cells in multiple sclerosis].
abstract: OBJECTIVE
  Тo investigate the possible association between clinical characteristics of multiple sclerosis (MS), quantitative and qualitative characteristics of Th17, dopamine and norepinephrine concentrations in the serum in patients with multiple sclerosis (MS).
  
  
  METHODS
  A comprehensive neurological and immunological examination of 43 patients with relapsing-remitting-MS (RR-MS) was performed. All patients were subjected to a standard neurological examination with assessment of the EDSS score. Dopamine and norepinephrine concentrations in serum were measured by enzyme-linked immunosorbent assay (ELISA). Percentage of Th17-cells was determined by flow cytometry. The functional activity of Th17- and Th1-cells was assessed by the production of interleukin-17 (IL-17) and interferon-gamma (IFN-γ), respectively, by peripheral blood mononuclear cells (PBMC) stimulated with microbeads coated with anti-CD3 and anti-CD28-antibodies.
  
  
  RESULTS
  The percent Th17-cells and cytokine production was significantly higher in MS patients with the exacerbation of disease than in the control group or remission, while the dopamine level was lower. Norepinephrine levels in MS patients in the acute stage and remission were comparable, but nevertheless, reliably lower than in the control group.
  
  
  CONCLUSIONS
  The results suggest the inhibitory effect of catecholamines on Th17 cells.
authors.affiliation: Pirogov Russian National Research Medical University, Moscow, Russia.
authors.forename: M V
authors.initials: MV
authors.lastname: Melnikov
authors.affiliation: Pirogov Russian National Research Medical University, Moscow, Russia.
authors.forename: O O
authors.initials: OO
authors.lastname: Belousova
authors.affiliation: Pirogov Russian National Research Medical University, Moscow, Russia.
authors.forename: R R
authors.initials: RR
authors.lastname: Zhetishev
authors.affiliation: Institute of Immunology, Moscow, Russia.
authors.forename: М V
authors.initials: МV
authors.lastname: Pashenkov
authors.affiliation: Pirogov Russian National Research Medical University, Moscow, Russia.
authors.forename: A N
authors.initials: AN
authors.lastname: Boyko
mesh_terms: D002395:Catecholamines / Q000032:analysis*; D004298:Dopamine; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis; D020381:Interleukin-17; D007963:Leukocytes, Mononuclear; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000188:drug therapy / Q000276:immunology*; D018417:Th1 Cells; D058504:Th17 Cells
chemical_list: D002395:Catecholamines; C508717:IL17A protein, human; D020381:Interleukin-17; D007371:Interferon-gamma; D004298:Dopamine