title: CD4+CD25hiCD127-Treg and CD4+CD45R0+CD49b+LAG3+Tr1 cells in bone marrow and peripheral blood samples from children with neuroblastoma.
abstract: Metastatic spread in the bone marrow (BM) at diagnosis is the worst prognostic factor for neuroblastoma (NB) patients. Here, we analyzed the presence of two immunosuppressive cell subsets, CD4+CD25hiCD127-regulatory T (Treg) cells and CD4+CD45R0+CD49b+LAG3+type 1 regulatory (Tr1) cells, in BM and peripheral blood (PB) samples from NB patients and controls. Frequency of both regulatory cell subsets was lower in BM and PB samples from NB patients than in respective healthy controls. No correlation was found between the frequency of Treg and Tr1 cells and prognostic factors at diagnosis, such as age and stage. OnlyMYCNamplification correlated to a higher number of Treg in BM and of Tr1 in PB. These findings suggested an altered trafficking of regulatory T cells in NB, but delineated a limited role of these subsets in BM microenvironment and/or periphery in NB. These observations should be considered designing immunotherapeutic approaches for metastatic NB.
authors.affiliation: Laboratorio di Oncologia, Istituto Giannina Gaslini , Genoa, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Morandi
authors.affiliation: Centro Cellule Staminali, IRCCS AOU San Martino-IST , Genoa, Italy.
authors.forename: Sarah
authors.initials: S
authors.lastname: Pozzi
authors.affiliation: Laboratorio Centrale di Analisi, Istituto Giannina Gaslini , Genoa, Italy.
authors.forename: Sebastiano
authors.initials: S
authors.lastname: Barco
authors.affiliation: Laboratorio Centrale di Analisi, Istituto Giannina Gaslini , Genoa, Italy.
authors.forename: Giuliana
authors.initials: G
authors.lastname: Cangemi
authors.affiliation: UOC Oncologia, Istituto Giannina Gaslini , Genoa, Italy.
authors.forename: Loredana
authors.initials: L
authors.lastname: Amoroso
authors.affiliation: Laboratorio di Oncologia, Istituto Giannina Gaslini , Genoa, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Carlini
authors.affiliation: Laboratorio di Oncologia, Istituto Giannina Gaslini, Genoa, Italy; Immunology Area, Ospedale Pediatrico Bambino Gesú, Rome, Italy.
authors.forename: Vito
authors.initials: V
authors.lastname: Pistoia
authors.affiliation: Laboratorio di Oncologia, Istituto Giannina Gaslini , Genoa, Italy.
authors.forename: Maria Valeria
authors.initials: MV
authors.lastname: Corrias
keywords: Neuroblastoma; Tr1; Treg; prognostic factors