title: Alleviation of lead-induced oxidative stress and immune damage by selenium in chicken bursa of Fabricius.
abstract: We investigated lead (Pb)-induced oxidative stress and immune damage in the chicken bursa of Fabricius (BF) and the ameliorative effect of selenium (Se). Seven-day-old male chickens were randomly divided into four groups and were provided standard diet and drinking water, Na2SeO3added to the standard diet and drinking water, standard diet and (CH3COO)2Pb added to drinking water, and Na2SeO3added to the standard diet and (CH3COO)2Pb added to drinking water for 30, 60, and 90 days. The presence of Pb inhibited total antioxidant capacity (T-AOC), glutathione peroxidase (GPx), glutathione S-transferase (GST), superoxide dismutase (SOD), and catalase (CAT) activities; decreased glutathione (GSH) content; increased malondialdehyde (MDA) and hydrogen peroxide (H2O2) contents; inhibited interleukin (IL)-2 and interferon-γ (IFN-γ) messenger RNA (mRNA) expression; and increased IL-4, IL-6, IL-10, IL-12β, and IL-17 mRNA expression. The presence of Se relieved all of the above Pb-induced changes. There were close correlations among GSH, CAT, T-AOC, SOD, GPx, MDA, and H2O2and among IL-2, IL-4, IL-6, IL-12β, IL-17, and IFN-γ. Our data showed that Pb caused oxidative stress and immune damage in the chicken BF. Se alleviated Pb-induced oxidative stress and immune damage in the chicken BF.
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Jiao
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: An
authors.affiliation: Grassland Workstation in Heilongjiang Province, Harbin, 150067, People's Republic of China. txj651007@163.com.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Teng
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People's Republic of China. tengxiaohua@neau.edu.cn.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Teng
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis; D002060:Bursa of Fabricius / Q000187:drug effects*; D002645:Chickens; D016207:Cytokines / Q000032:analysis; D007854:Lead / Q000633:toxicity*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D012643:Selenium / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D016207:Cytokines; D007854:Lead; D012643:Selenium
keywords: Bursa of Fabricius; Chicken; Cytokine; Lead; Oxidative stress; Selenium