title: Biosynthesis of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) by bacterial community from propylene oxide saponification wastewater residual sludge.
abstract: The saponification wastewater from the process of propylene oxide (PO) production is contaminated with high chemical oxygen demand (COD) and chlorine contents. Although the activated sludge process could treat the PO saponification wastewater effectively, the residual sludge was difficult to be disposed properly. In this research, microbes in PO saponification wastewater residual sludge were acclimated to produce poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) from volatile fatty acids. Through Miseq Illumina highthroughput sequencing, the bacterial community discrepancy between the original and the acclimated sludge samples were analyzed. The proportions of Bacillus, Acinetobacter, Brevundimonas and Pseudomonas, the potential PHBV-producers in the residual sludge, were all obviously increased. In the batch fermentation, the production of PHBV could achieve 4.262g/L at 300min, with the content increased from 0.04% to 23.67% of mixed liquor suspended solid (MLSS) in the acclimated sludge, and the COD of the PO saponification wastewater was also decreased in the fermentation. This work would provide an effective solution for the utilization of PO saponification wastewater residual sludge.
authors.affiliation: School of Biological Science and Technology, University of Jinan, Jinan, China.
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: New Materials Research Institute of Shandong Academy of Sciences, Jinan, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: School of Biological Science and Technology, University of Jinan, Jinan, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Gu
authors.affiliation: School of Biological Science and Technology, University of Jinan, Jinan, China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Biological Science and Technology, University of Jinan, Jinan, China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Fan
authors.affiliation: School of Biological Science and Technology, University of Jinan, Jinan, China.
authors.forename: Dongxue
authors.initials: D
authors.lastname: Song
authors.affiliation: School of Biological Science and Technology, University of Jinan, Jinan, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ji
authors.affiliation: School of Biological Science and Technology, University of Jinan, Jinan, China. Electronic address: chm_liq@ujn.edu.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
mesh_terms: D000085:Acetates / Q000378:metabolism; D001419:Bacteria / Q000378:metabolism*; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology; D004852:Epoxy Compounds / Q000378:metabolism*; D005285:Fermentation; D011091:Polyesters / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology*; D000094122:Polyhydroxybutyrates
chemical_list: D000085:Acetates; D004852:Epoxy Compounds; D011091:Polyesters; D012722:Sewage; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C009068:propylene oxide
keywords: Bacterial community; Poly (3-hydroxybutyrate-co-3-hydroxyvalerate); Propylene oxide saponification wastewater