title: Phenolic composition of pomegranate peel extracts using an liquid chromatography-mass spectrometry approach with silica hydride columns.
abstract: The peels of different pomegranate cultivars (Molla Nepes, Parfianka, Purple Heart, Wonderful and Vkunsyi) were compared in terms of phenolic composition and total phenolics. Analyses were performed on two silica hydride based stationary phases: phenyl and undecanoic acid columns. Quantitation was accomplished by developing a liquid chromatography with mass spectrometry approach for separating different phenolic analytes, initially in the form of reference standards and then with pomegranate extracts. The high-performance liquid chromatography columns used in the separations had the ability to retain a wide polarity range of phenolic analytes, as well as offering beneficial secondary selectivity mechanisms for resolving the isobaric compounds, catechin and epicatechin. The Vkunsyi peel extract had the highest concentration of phenolics (as determined by liquid chromatography with mass spectrometry) and was the only cultivar to contain the important compound punicalagin. The liquid chromatography with mass spectrometry data were compared to the standard total phenolics content as determined by using the Folin-Ciocalteu assay.
authors.affiliation: MicroSolv Technology Corporation, Leland, NC, USA.
authors.forename: Joshua E
authors.initials: JE
authors.lastname: Young
authors.affiliation: Department of Biological and Agricultural Engineering, University of California, Davis, CA, USA.|United States Department of Agriculture, Agricultural Research Service, Healthy Processed Foods Research Unit, Albany, CA, USA.
authors.forename: Zhongli
authors.initials: Z
authors.lastname: Pan
authors.affiliation: San José State University, San Jose, CA, USA.
authors.forename: Hui Ean
authors.initials: HE
authors.lastname: Teh
authors.affiliation: San José State University, San Jose, CA, USA.
authors.forename: Veena
authors.initials: V
authors.lastname: Menon
authors.affiliation: San José State University, San Jose, CA, USA.
authors.forename: Brent
authors.initials: B
authors.lastname: Modereger
authors.affiliation: San José State University, San Jose, CA, USA.
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Pesek
authors.affiliation: San José State University, San Jose, CA, USA.
authors.forename: Maria T
authors.initials: MT
authors.lastname: Matyska
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Healthy Processed Foods Research Unit, Albany, CA, USA.
authors.forename: Lan
authors.initials: L
authors.lastname: Dao
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Healthy Processed Foods Research Unit, Albany, CA, USA.
authors.forename: Gary
authors.initials: G
authors.lastname: Takeoka
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000295:instrumentation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D029561:Lythraceae / Q000737:chemistry*; D013058:Mass Spectrometry; D010636:Phenols / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D017640:Silicates / Q000737:chemistry*
chemical_list: D010636:Phenols; D010936:Plant Extracts; D017640:Silicates; C487012:silica hydride
keywords: Folin-Ciocalteu assay; isobaric compound separation; phenyl hydride stationary phases