title: In Vivo Analysis of Hypertension: Induction of Hypertension, In Vivo Kinase Manipulation, and Assessment of Physiologic Outputs.
abstract: Using an in vivo model system to study signal transduction will include several steps: (1) induce hypertension in the animal, (2) manipulate kinase activation and signal transduction pathways as desired, and (3) observe physiologic outputs. This chapter provides the reader with overviews of the techniques our lab uses to manipulate signal transduction pathways and determine the effects on hypertension.
authors.affiliation: Department of Physiology, Cardiovascular Research Centre, Lewis Katz School of Medicine at Temple University, 3500 Nth Board St, Philadelphia, PA, 19140, USA. seguchi@Temple.edu.
authors.forename: Satoru
authors.initials: S
authors.lastname: Eguchi
authors.affiliation: Department of Physiology, Cardiovascular Research Centre, Lewis Katz School of Medicine at Temple University, 3500 Nth Board St, Philadelphia, PA, 19140, USA.
authors.forename: Katherine
authors.initials: K
authors.lastname: Elliott
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D066246:ErbB Receptors / Q000378:metabolism; D006973:Hypertension / Q000378:metabolism* / Q000503:physiopathology; D007150:Immunohistochemistry; D051379:Mice; D010766:Phosphorylation / Q000187:drug effects; D012084:Renin-Angiotensin System / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000804:Angiotensin II; D066246:ErbB Receptors
keywords: Angiotensin II; EGF receptor; End organ damage; Fibrosis; Hypertension; Hypertrophy