title: Nanothermometry Measure of Muscle Efficiency.
abstract: Despite recent advances in thermometry, determination of temperature at the nanometer scale in single molecules to live cells remains a challenge that holds great promise in disease detection among others. In the present study, we use a new approach to nanometer scale thermometry with a spatial and thermal resolution of 80 nm and 1 mK respectively, by directly associating 2 nm cadmium telluride quantum dots (CdTe QDs) to the subject under study. The 2 nm CdTe QDs physically adhered to bovine cardiac and rabbit skeletal muscle myosin, enabling the determination of heat released when ATP is hydrolyzed by both myosin motors. Greater heat loss reflects less work performed by the motor, hence decreased efficiency. Surprisingly, we found rabbit skeletal myosin to be more efficient than bovine cardiac. We have further extended this approach to demonstrate the gain in efficiency of Drosophila melanogaster skeletal muscle overexpressing the PGC-1α homologue spargel, a known mediator of improved exercise performance in humans. Our results establish a novel approach to determine muscle efficiency with promise for early diagnosis and treatment of various metabolic disorders including cancer.
authors.affiliation: Department of Physiology, Wayne State University School of Medicine , Detroit, Michigan 48201, United States.
authors.forename: Suvra S
authors.initials: SS
authors.lastname: Laha
authors.affiliation: Department of Physiology, Wayne State University School of Medicine , Detroit, Michigan 48201, United States.
authors.forename: Akshata R
authors.initials: AR
authors.lastname: Naik
authors.affiliation: Department of Physiology, Wayne State University School of Medicine , Detroit, Michigan 48201, United States.
authors.forename: Eric R
authors.initials: ER
authors.lastname: Kuhn
authors.affiliation: Department of Physiology, Wayne State University School of Medicine , Detroit, Michigan 48201, United States.
authors.forename: Maysen
authors.initials: M
authors.lastname: Alvarez
authors.affiliation: Department of Physiology, Wayne State University School of Medicine , Detroit, Michigan 48201, United States.
authors.forename: Alyson
authors.initials: A
authors.lastname: Sujkowski
authors.affiliation: Department of Physiology, Wayne State University School of Medicine , Detroit, Michigan 48201, United States.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Wessells
authors.affiliation: Department of Physiology, Wayne State University School of Medicine , Detroit, Michigan 48201, United States.
authors.forename: Bhanu P
authors.identifier: 0000-0002-6030-8766
authors.initials: BP
authors.lastname: Jena
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry; D000818:Animals; D019187:Cadmium Compounds / Q000737:chemistry*; D024722:Cardiac Myosins / Q000737:chemistry*; D002417:Cattle; D004331:Drosophila melanogaster / Q000502:physiology; D005453:Fluorescence; D006868:Hydrolysis; D008297:Male; D018482:Muscle, Skeletal / Q000502:physiology*; D036103:Nanotechnology; D010316:Particle Size; D045663:Quantum Dots / Q000737:chemistry*; D011817:Rabbits; D024744:Skeletal Muscle Myosins / Q000737:chemistry* / Q000502:physiology; D013499:Surface Properties; D013691:Tellurium / Q000737:chemistry*; D013696:Temperature; D062125:Thermometry
chemical_list: D019187:Cadmium Compounds; D000255:Adenosine Triphosphate; D024722:Cardiac Myosins; D024744:Skeletal Muscle Myosins; D013691:Tellurium; C028337:cadmium telluride
keywords: Nanometer scale thermometry; disease detection; muscle efficiency; quantum dot