title: Effect of Chitosan Physical Form on Its Antibacterial Activity Against Pathogenic Bacteria.
abstract: The antibacterial activity of chitosan (CS) nanospheres, in comparison with other physical forms, was investigated against Salmonella enterica serovar Typhimurium and Staphylococcus aureus, which are 2 foodborne harmful pathogens. Results showed that the antibacterial efficacy of CS nanospheres: (1) was superior to that displayed by CS in powder and solution form; (2) was higher against S. aureus than against Salmonella Typhimurium; and (3) was dependent on the temperature and pH of the medium depending on the strain. For S. Typhimurium, a higher activity was displayed at 37 °C, in which 99.9% of the population was eradicated independently of the pH, followed by 20 °C and 7 °C, in which acidic pH conditions favored a higher susceptibility of bacteria to the effect of CS. On the contrary, S. aureus was less susceptible to the pH and temperature conditions of the medium, and no statistical difference in the antibacterial effect was observed for pH 5.8 and 8.0 at 20 °C and 37 °C. However, at 7 °C a slightly higher activity was displayed at pH 5.8 than at 8.0.
authors.affiliation: CREPEC, Dept. of Chemical Engineering, Polytechnique Montréal, P.O. Box 6079, Station Centre-Ville, Montréal, Québec, Canada, H3C 3A7.
authors.forename: Nury
authors.initials: N
authors.lastname: Ardila
authors.affiliation: Dept. of Microbiology, Infectiology and Immunology, Pavillon Roger-Gaudry, Univ. de Montréal, P.O. Box 6128, Station Centre-ville, Montréal, Québec, Canada, H3C 3J7.
authors.forename: France
authors.initials: F
authors.lastname: Daigle
authors.affiliation: CREPEC, Dept. of Chemical Engineering, Polytechnique Montréal, P.O. Box 6079, Station Centre-Ville, Montréal, Québec, Canada, H3C 3A7.
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Heuzey
authors.affiliation: CREPEC, Dept. of Chemical Engineering, Polytechnique Montréal, P.O. Box 6079, Station Centre-Ville, Montréal, Québec, Canada, H3C 3A7.
authors.forename: Abdellah
authors.initials: A
authors.lastname: Ajji
mesh_terms: D000143:Acids / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology; D048271:Chitosan / Q000008:administration & dosage* / Q000494:pharmacology; D015169:Colony Count, Microbial; D005517:Foodborne Diseases / Q000382:microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles; D012486:Salmonella typhimurium / Q000187:drug effects*; D013211:Staphylococcus aureus / Q000187:drug effects*; D013696:Temperature
chemical_list: D000143:Acids; D000900:Anti-Bacterial Agents; D048271:Chitosan
keywords: S. Typhimurium; S. aureus; antibacterial activity; chitosan; physical form