title: Influences of LaCl3on the mineral phase transformation during osteoblast mineralization in vitro.
abstract: Rat calvarial osteoblasts were treated with lanthanum chloride (LaCl3) to explore its effect on the mineral crystalline phase during the process of osteoblast calcification in vitro. The results confirmed that La was readily deposited in the mineral component of the matrix. Employing high-resolution transmission electron microscopy and Fourier transform infrared microspectroscopy techniques, we demonstrated that features comparable to dicalcium phosphate dihydrate (DCPD) and octacalcium phosphate, and hydroxyapatite (HAP) were detected in the mineral phases in vitro. Particularly, LaCl3treatment retarded conversion from DCPD-like phase into HAP during mineralization. In addition, La was introduced in DCPD powder during wet chemical synthesis. When compared with that of La-free DCPD, the dissolution rate of La-incorporated DCPD was lower, thereby leading to a delayed DCPD-to-HAP phase transformation. Thus, it can be concluded that LaCl3treatment influences the kinetics of inorganic phase transition by decreasing the dissolution rate of DCPD.
authors.affiliation: Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China. Electronic address: yucaixia0105@163.com.
authors.forename: Caixia
authors.initials: C
authors.lastname: Yu
authors.affiliation: Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Gong
authors.affiliation: Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Fu-Ling
authors.initials: FL
authors.lastname: Yin
authors.affiliation: Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China. Electronic address: jhuang@bjmu.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Tian-Lan
authors.initials: TL
authors.lastname: Zhang
authors.affiliation: Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Kui
authors.initials: K
authors.lastname: Wang
mesh_terms: D000818:Animals; D002130:Calcium Phosphates / Q000737:chemistry; D017886:Durapatite; D007700:Kinetics; D007811:Lanthanum / Q000737:chemistry*; D008956:Models, Chemical; D010006:Osteoblasts / Q000502:physiology*; D051381:Rats
chemical_list: D002130:Calcium Phosphates; C028521:lanthanum chloride; C022045:octacalcium phosphate; D007811:Lanthanum; D017886:Durapatite; C494366:calcium phosphate, dibasic, dihydrate
keywords: Dicalcium phosphate dehydrate; Lanthanum; Mineralization; Osteoblasts; Transformation