title: Experimental Arthritis in the Rat Induced by the Superantigen Staphylococcal Enterotoxin A.
abstract: The pathogenesis of rheumatoid arthritis (RA) is incompletely understood. Human endogenous retroviruses (HERVs) and their superantigenic envelope protein (env) have been implicated in the pathogenesis of RA. In the present investigation, the arthritogenic potential of the superantigen staphylococcal enterotoxin A (SEA) has been investigated. In the present investigation, the bacterial superantigen staphylococcal enterotoxin A (SEA) was injected into the right knee joint of 15 Lewis rats. Further nine animals received saline. Animals were sacrificed one, five and 10 days after the injection, respectively. The antigens CD3, CD4, CD8, MHC class I, MHC class II, Pax5 and CD138 were investigated by immunohistochemistry on cryo-sections. After intra-articular SEA injection, the inflammation was initially dominated by CD8+ T cells. In the course of the investigation, the numbers of CD4+, Pax5+, CD138+ and MHC class II+ cells increased. CD3 was expressed in low numbers as compared to CD8. After saline injection, no similar inflammatory response has been detected. The arthritis induced by the superantigen SEA may be a novel model for inflammatory joint diseases, that is rheumatoid arthritis or juvenile idiopathic arthritis.
authors.affiliation: Department of Paediatric Surgery, Martin Luther University Halle-Wittenberg, Halle, Germany.|Department of Paediatrics, Martin Luther University Halle-Wittenberg, Halle, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Gerlach
authors.affiliation: Department of Paediatric Surgery, Martin Luther University Halle-Wittenberg, Halle, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Tomuschat
authors.affiliation: Department of Paediatric Surgery, Martin Luther University Halle-Wittenberg, Halle, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Finke
authors.affiliation: Department of Paediatrics, Martin Luther University Halle-Wittenberg, Halle, Germany.
authors.forename: M S
authors.initials: MS
authors.lastname: Staege
authors.affiliation: Department of Paediatrics, Martin Luther University Halle-Wittenberg, Halle, Germany.|Department of Neurology, Martin Luther University Halle-Wittenberg, Halle, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Brütting
authors.affiliation: Department of Orthopaedics, Martin Luther University Halle-Wittenberg, Halle, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Brandt
authors.affiliation: Department of Neurology, Martin Luther University Halle-Wittenberg, Halle, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Jordan
authors.affiliation: Department of Orthopaedics, Martin Luther University Halle-Wittenberg, Halle, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Schwesig
authors.affiliation: Department of Orthopaedics, Martin Luther University Halle-Wittenberg, Halle, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Rosemeier
authors.affiliation: Department of Orthopaedics, Martin Luther University Halle-Wittenberg, Halle, Germany.
authors.forename: K-S
authors.initials: KS
authors.lastname: Delank
authors.affiliation: Department of Neurology, Martin Luther University Halle-Wittenberg, Halle, Germany.
authors.forename: M E
authors.initials: ME
authors.lastname: Kornhuber
authors.affiliation: Department of Neurology, Martin Luther University Halle-Wittenberg, Halle, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Emmer
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000276:immunology* / Q000473:pathology*; D001172:Arthritis, Rheumatoid / Q000473:pathology; D001402:B-Lymphocytes / Q000276:immunology; D017252:CD3 Complex / Q000378:metabolism; D015704:CD4 Antigens / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D016827:CD8 Antigens / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D004195:Disease Models, Animal; D004768:Enterotoxins / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D008297:Male; D051757:PAX5 Transcription Factor / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D018089:Superantigens / Q000276:immunology*; D053668:Syndecan-1 / Q000378:metabolism
chemical_list: D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D004768:Enterotoxins; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D051757:PAX5 Transcription Factor; D018089:Superantigens; D053668:Syndecan-1; C026066:enterotoxin A, Staphylococcal