title: Characterization of the Bujaru, frijoles and Tapara antigenic complexes into the sandfly fever group and two unclassified phleboviruses from Brazil.
abstract: The genus Phlebovirus includes the sandfly fever viruses and tick-transmitted uukuviruses. Sandfly fever group viruses have been isolated from various vertebrate species and from phlebotomines and occasionally alternative arthropods, e.g. mosquitoes, or ceratopogonids of the genus Culicoides. Uukuniemi serogroup viruses have been isolated from various vertebrate species and from ticks. Despite the public health importance of some viruses of the genus, the genomic diversity of phleboviruses that could be incriminated as causative of human or veterinary diseases remains underestimated. Here we describe the nearly complete sequences and genomic characterization of two phleboviruses belonging to the Bujaru antigenic complex: the prototype species and the Munguba virus. Furthermore, six previously unclassified phleboviruses isolated in Brazil were also sequenced and characterized: Ambe, Anhanga, Joa, Uriurana, Urucuri and Tapara viruses. The results of the phylogenetic analysis indicated that these viruses group with viruses of three antigenic complexes (Bujaru, Tapara and frijoles clades), with two unclassified phleboviruses. We also performed genomic reassortment analysis and confirmed that there were no events for the viruses described in this study, but we found a new potential reassortment in Medjerda Valley virus, which contains S and L segments of Arbia virus, and probably a unique M segment, both viruses circulate in the same geographic region, indicating these two isolates represent two distinct viruses. This study provides insights into the genetic diversity, classification and evolution of phleboviruses.
authors.affiliation: Departamento de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Ananindeua, Pará, Brazil.
authors.forename: Joaquim Pinto
authors.initials: JP
authors.lastname: Nunes-Neto
authors.affiliation: Virology Research Center, School of Medicine of Ribeirao Preto of University of São Paulo, Ribeirao Preto, São Paulo, Brazil.|MRC-University of Glasgow Centre for Virus Research, Glasgow, Scotland, UK.
authors.forename: William Marciel de
authors.initials: WM
authors.lastname: Souza
authors.affiliation: Universidade Federal da Fronteira Sul, Passo Fundo, Rio Grande do Sul, Brazil.
authors.forename: Gustavo Olszanski
authors.initials: GO
authors.lastname: Acrani
authors.affiliation: Virology Research Center, School of Medicine of Ribeirao Preto of University of São Paulo, Ribeirao Preto, São Paulo, Brazil.
authors.forename: Marilia Farignoli
authors.initials: MF
authors.lastname: Romeiro
authors.affiliation: Virology Research Center, School of Medicine of Ribeirao Preto of University of São Paulo, Ribeirao Preto, São Paulo, Brazil.
authors.forename: MarcílioJorge
authors.initials: M
authors.lastname: Fumagalli
authors.affiliation: Virology Research Center, School of Medicine of Ribeirao Preto of University of São Paulo, Ribeirao Preto, São Paulo, Brazil.
authors.forename: Luiz Carlos
authors.initials: LC
authors.lastname: Vieira
authors.affiliation: Departamento de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Ananindeua, Pará, Brazil.
authors.forename: Daniele Barbosa de Almeida
authors.initials: DBA
authors.lastname: Medeiros
authors.affiliation: Departamento de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Ananindeua, Pará, Brazil.
authors.forename: Juliana Abreu
authors.initials: JA
authors.lastname: Lima
authors.affiliation: Center for Technological Innovation, Instituto Evandro Chagas, Ananindeua, Pará, Brazil.
authors.forename: Clayton Pereira Silva de
authors.initials: CPS
authors.lastname: Lima
authors.affiliation: Center for Technological Innovation, Instituto Evandro Chagas, Ananindeua, Pará, Brazil.
authors.forename: Jedson Ferreira
authors.initials: JF
authors.lastname: Cardoso
authors.affiliation: Virology Research Center, School of Medicine of Ribeirao Preto of University of São Paulo, Ribeirao Preto, São Paulo, Brazil.
authors.forename: Luiz Tadeu Moraes
authors.initials: LTM
authors.lastname: Figueiredo
authors.affiliation: Center for Technological Innovation, Instituto Evandro Chagas, Ananindeua, Pará, Brazil.
authors.forename: Sandro Patroca da
authors.initials: SPD
authors.lastname: Silva
authors.affiliation: Department of Pathology, University of Texas Medical Branch, Galveston Texas, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Tesh
authors.affiliation: Departamento de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Ananindeua, Pará, Brazil.|Center for Technological Innovation, Instituto Evandro Chagas, Ananindeua, Pará, Brazil.|Department of Pathology, University of Texas Medical Branch, Galveston Texas, USA.
authors.forename: Márcio Roberto Teixeira
authors.initials: MRT
authors.lastname: Nunes
authors.affiliation: Departamento de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Ananindeua, Pará, Brazil.|Department of Pathology, Pará State University, Belém, Pará, Brazil.
authors.forename: Pedro Fernando da Costa
authors.initials: PFDC
authors.lastname: Vasconcelos
mesh_terms: D000818:Animals; D001938:Brazil; D016000:Cluster Analysis; D014644:Genetic Variation; D016679:Genome, Viral; D016856:Phlebovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D011576:Psychodidae / Q000821:virology; D016865:Reassortant Viruses / Q000235:genetics; D012377:Rodentia / Q000821:virology; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D004490:Xenarthra / Q000821:virology