title: Physical mapping of immune-related genes in Yesso scallop (Patinopecten yessoensis) using fluorescentin situhybridization.
abstract: The innate immune system plays a pivotal role in defending invasion of microorganisms for scallops. Previous studies on immune-related genes in the Yesso scallop,Patinopecten yessoensis(Jay, 1857) have mainly focused on characterization and expression pattern in response to bacterial challenge, no research has been carried out on the cytogenetic level yet. In the present study, eight fosmid clones containing the sequences of key immune-related genes (PyNFkB,PyTRAF2,PyTRAF4,PyTRAF7,PyMyd88-1,PyMyd88-3,PyMKK-7 andPyTNFR) were isolated and seven of them were successfully mapped on chromosomes ofPatinopecten yessoensisutilizing fluorescencein situhybridization. Wherein,PyMyd88-1,PyMyd88-3 andPyMKK-7 located on the same chromosome pair with adjacent positions and the other genes were mapped on four non-homologous chromosome pairs, showing a similar distribution to another five model species. The isolation and mapping of such genes of the Yesso scallop will lay a foundation for studies such as assignment of interested genes to chromosomes, construction cytogenetic maps and so on.
authors.affiliation: Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China.
authors.forename: Zujing
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Liao
authors.affiliation: Yantai Fisheries Research Institute, Yantai 264003, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Hu
authors.affiliation: Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China.
authors.forename: Zhengrui
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Bosong
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China.
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Huang
authors.affiliation: Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China.
authors.forename: Zhenmin
authors.initials: Z
authors.lastname: Bao
keywords: Cytogenetic map; FISH; fosmid; immune-related genes