title: Nucleoplasmic bridges as a biomarker of DNA damage exposed to radon.
abstract: Radon is a naturally occurring radionuclide in the environment, during decay it emits high linear energy transfer (LET) alpha particles. When radon exposure is accompanied by smoking it has been reported that lung cancer risk is higher. Blood samples were collected after prior consent, 25 smokers and 25 non smokers (only males) exposed in vitro to radon gas with doses ranging between 0.3-12.6mGy Ionizing radiation is a strong clastogenic agent and a potent inducer of MN. Cytokinesis-Blocked Micro Nucleus (CBMN) assay has proven to be a reliable, thoroughly validated and standardised technique in the field of radiation biology. In view of this, an invitro study has been undertaken using CBMN assay to suggest Nucleoplasmic bridges (NPBs) as a potential bio-dosimeter and to investigate the existence of a possible influence of smoking on genetic damage induced by ionizing radiation. The mean percentages frequencies of micronuclei, nucleoplasmic bridges and nuclear buds among the exposed smoker group were 4.82±0.271, 0.6±0.04 and 0.12±0.02 respectively and these values for exposed non-smoker group were 2.78±0.285, 0.4±0.04 and 0.06±0.01 respectively. Spearman rank correlation for the frequency of nucleoplasmic bridge with respect to dose was considered to be significant (P<0.05) in non-smokers. This study is first of its kind to investigate NPB as a biomarker of early DNA damage induced by radon.
authors.affiliation: Radiological Safety Division, Indira Gandhi Centre for Atomic Research (IGCAR), Kalpakkam, 603102, Tamilnadu, India. Electronic address: c.meenakshi02@gmail.com.
authors.forename: C
authors.initials: C
authors.lastname: Meenakshi
authors.affiliation: Radiological Safety Division, Indira Gandhi Centre for Atomic Research (IGCAR), Kalpakkam, 603102, Tamilnadu, India.
authors.forename: K
authors.initials: K
authors.lastname: Sivasubramanian
authors.affiliation: Radiological Safety Division, Indira Gandhi Centre for Atomic Research (IGCAR), Kalpakkam, 603102, Tamilnadu, India.
authors.forename: B
authors.initials: B
authors.lastname: Venkatraman
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism*; D002467:Cell Nucleus / Q000528:radiation effects*; D004249:DNA Damage; D006801:Humans; D008297:Male; D008875:Middle Aged; D011886:Radon / Q000633:toxicity*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D011886:Radon
keywords: DNA damage; Micronucleus; Nucleoplasmic bridges; Radon; Smokers