title: Extracellular Nucleotide Hydrolysis in Dermal and Limbal Mesenchymal Stem Cells: A Source of Adenosine Production.
abstract: Human Limbal (L-MSCs) and Dermal Mesenchymal Stem Cell (D-MSCs) possess many properties that increase their therapeutic potential in ophthalmology and dermatology. It is known that purinergic signaling plays a role in many aspects of mesenchymal stem cells physiology. They release and respond to purinergic ligands, altering proliferation, migration, differentiation, and apoptosis. Therefore, more information on these processes would be crucial for establishing future clinical applications using their differentiation potential, but without undesirable side effects. This study evaluated and compared the expression of ecto-nucleotidases, the enzymatic activity of degradation of extracellular nucleotides and the metabolism of extracellular ATP in D-MSCs and L-MSCs, isolated from discard tissues of human skin and sclerocorneal rims. The D-MSCs and L-MSCs showed a differentiation potential into osteogenic, adipogenic, and chondrogenic lineages and the expression of markers CD105+, CD44+, CD14-, CD34-, CD45-, as expected. Both cells hydrolyzed low levels of extracellular ATP and high levels of AMP, leading to adenosine accumulation that can regulate inflammation and tissue repair. These cells expressed mRNA for ENTPD1, 2, 3, 5 and 6, and CD73 that corresponded to the observed enzymatic activities. Thus, considering the degradation of ATP and adenosine production, limbal MSCs are very similar to dermal MSCs, indicating that from the aspect of extracellular nucleotide metabolism L-MSCs are very similar to the characterized D-MSCs. J. Cell. Biochem. 118: 2430-2442, 2017. © 2017 Wiley Periodicals, Inc.
authors.affiliation: Departamento de Ciências Básicas da Saúde e Laboratório de Biologia Celular, Universidade Federal de Ciências da Saúde de Porto Alegre-UFCSPA, Porto Alegre, RS, Brasil.
authors.forename: Liliana I Sous
authors.initials: LIS
authors.lastname: Naasani
authors.affiliation: Departamento de Ciências Básicas da Saúde e Laboratório de Biologia Celular, Universidade Federal de Ciências da Saúde de Porto Alegre-UFCSPA, Porto Alegre, RS, Brasil.
authors.forename: Cristiano
authors.initials: C
authors.lastname: Rodrigues
authors.affiliation: Departamento de Ciências Básicas da Saúde e Laboratório de Biologia Celular, Universidade Federal de Ciências da Saúde de Porto Alegre-UFCSPA, Porto Alegre, RS, Brasil.
authors.forename: Rafael Paschoal
authors.initials: RP
authors.lastname: de Campos
authors.affiliation: Departamento de Ciências Básicas da Saúde e Laboratório de Biologia Celular, Universidade Federal de Ciências da Saúde de Porto Alegre-UFCSPA, Porto Alegre, RS, Brasil.
authors.forename: Liziane Raquel
authors.initials: LR
authors.lastname: Beckenkamp
authors.affiliation: Departamento de Ciências Básicas da Saúde e Laboratório de Biologia Celular, Universidade Federal de Ciências da Saúde de Porto Alegre-UFCSPA, Porto Alegre, RS, Brasil.
authors.forename: Isabele C
authors.initials: IC
authors.lastname: Iser
authors.affiliation: Departamento de Ciências Básicas da Saúde e Laboratório de Biologia Celular, Universidade Federal de Ciências da Saúde de Porto Alegre-UFCSPA, Porto Alegre, RS, Brasil.
authors.forename: Ana Paula Santin
authors.initials: APS
authors.lastname: Bertoni
authors.affiliation: Departamento de Ciências Básicas da Saúde e Laboratório de Biologia Celular, Universidade Federal de Ciências da Saúde de Porto Alegre-UFCSPA, Porto Alegre, RS, Brasil.
authors.forename: Márcia R
authors.identifier: 0000-0001-5987-3509
authors.initials: MR
authors.lastname: Wink
mesh_terms: D000241:Adenosine / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics / Q000502:physiology; D018952:Antigens, CD34 / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D020219:Chondrogenesis / Q000235:genetics / Q000502:physiology; D000071063:Endoglin / Q000235:genetics / Q000378:metabolism; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics / Q000378:metabolism; D006868:Hydrolysis; D017493:Leukocyte Common Antigens / Q000235:genetics / Q000378:metabolism; D016850:Limbus Corneae / Q000166:cytology; D018950:Lipopolysaccharide Receptors / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D009711:Nucleotides / Q000378:metabolism*; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics / Q000502:physiology; D011755:Pyrophosphatases / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000166:cytology
chemical_list: D018952:Antigens, CD34; D000071063:Endoglin; D018960:Hyaluronan Receptors; D018950:Lipopolysaccharide Receptors; D009711:Nucleotides; D015513:Oncogene Proteins; D000255:Adenosine Triphosphate; D017493:Leukocyte Common Antigens; D000251:Adenosine Triphosphatases; C117634:ENTPD5 protein, human; D011755:Pyrophosphatases; C027201:ectoATPase; D000241:Adenosine
keywords: CD73; DERMAL MESENCHYMAL STEM CELLS; LIMBAL MESENCHYMAL STEM CELLS; MSCs; NTPDases; PURINERGIC SIGNALING