title: An In Vitro Evaluation of Ozonized Organic Extra-Virgin Olive Oil onGiardia LambliaCysts.
abstract: BACKGROUND
  Giardia lambliais a common intestinal parasite that has been reported all over the world.
  
  
  OBJECTIVE
  This study was conducted to evaluate the effect of ozonized organic extra-virgin olive oil on the cyst ofG. lamblia.
  
  
  METHODS
  The olive oil was ozonized based on international standards and confirmed by the world health organization (WHO) at various times in a generator. The ozone concentration of olive oil was adjusted at 32, 64, 96, 128, 160 mg/g based on ozone absorption.Giardia lambliacysts were isolated from heavily infected stool samples and the sucrose gradient flotation technique. Five groups of triple tubes containingGiardiacysts were exposed to olive oil with 32, 64, 96, 128, 160 ozone concentrations, and the sixth and seventh groups were exposed to non-ozonized olive oil and normal saline, respectively. The tubes were placed at room temperature, and every four hours, the mortality of theGiardiacysts was assessed.
  
  
  RESULTS
  The results showed that the first five groups' mortality rate ofGiardiacysts reached 100% in 100 hours. An increasing concentration of ozone in olive oil leads to an increase in the mortality rate ofGiardiacysts. The results showed a significant difference in the mean time of the mortality in all the groups (P ≤ 0.05). Furthermore, the higher fatality effect of ozonized organic extra-virgin olive oil (Ozonized Olive Oil = OZO) was proved in comparison with metronidazole in vitro.
  
  
  CONCLUSIONS
  We concluded that ozonized organic extra-virgin olive oil was a growth inhibitor ofGiardiacysts, and concerning its compatibility with a biological system, it is recommended for further clinical trials.
authors.affiliation: Infectious Diseases Research Center, School of Medicine, Arak University of Medical Sciences, Arak, IR Iran.
authors.forename: Najmeh Sadat
authors.initials: NS
authors.lastname: Boland-Nazar
authors.affiliation: Department of Parasitology and Mycology, School of Medicine, Arak University of Medical Sciences, Arak, IR Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Eslamirad
authors.affiliation: Infectious Diseases Research Center, School of Medicine, Arak University of Medical Sciences, Arak, IR Iran; Department of Infection Disease, School of Medicine, Arak University of Medical Sciences, Arak, IR Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Sarmadian
authors.affiliation: Infectious Diseases Research Center, School of Medicine, Arak University of Medical Sciences, Arak, IR Iran; Department of Parasitology and Mycology, School of Medicine, Arak University of Medical Sciences, Arak, IR Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Ghasemikhah
keywords: Complementary Therapies; Giardia lamblia; Olive Plant; Ozone; Ozonized Olive Oil