title: Combined application of chromatographic techniques for the separation of phenolic compounds from Stenoloma chusanum Ching.
abstract: High-speed countercurrent chromatography combined with preparative high-performance liquid chromatography was successfully used to separate seven phenolic compounds from Stenoloma chusanum Ching. A biphasic solvent system composed of hexane/ethyl acetate/methanol/water (1:2:1:2, v/v) was used for the first step high-speed countercurrent chromatography separation in elution-extrusion mode. A mobile phase composed of acetonitrile (18%) and pure water (82%) was used for further preparative high-performance liquid chromatography purification. In total, the combined separation yielded seven compounds, including 3,4-dihydroxy benzoic acid, 3,4-dihydroxy benzaldehyde, esculetin, caffeic acid, syringic acid, luteolin, and apigenin, at a purity of over 90%. Esculetin was separated from Stenoloma chusanum Ching for the first time. The results suggest that the proposed combination method is a useful strategy for separating compounds from complex samples.
authors.affiliation: Yunnan Food Safety Research Institute, Kunming University of Science and Technology, Kunming, P.R. China.
authors.forename: Dabing
authors.initials: D
authors.lastname: Ren
authors.affiliation: Yunnan Food Safety Research Institute, Kunming University of Science and Technology, Kunming, P.R. China.
authors.forename: Binsong
authors.initials: B
authors.lastname: Han
authors.affiliation: Yunnan Food Safety Research Institute, Kunming University of Science and Technology, Kunming, P.R. China.
authors.forename: Zhongquan
authors.initials: Z
authors.lastname: Xin
authors.affiliation: Yunnan Food Safety Research Institute, Kunming University of Science and Technology, Kunming, P.R. China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Ma
authors.affiliation: Yunnan Food Safety Research Institute, Kunming University of Science and Technology, Kunming, P.R. China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Liu
authors.affiliation: Yunnan Food Safety Research Institute, Kunming University of Science and Technology, Kunming, P.R. China.
authors.forename: Lunzhao
authors.initials: L
authors.lastname: Yi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution; D000432:Methanol; D010636:Phenols / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry*; D064028:Tracheophyta / Q000737:chemistry*
chemical_list: D010636:Phenols; D010936:Plant Extracts; D000432:Methanol
keywords: Stenoloma chusanum Ching; countercurrent chromatography; phenolic compounds; preparative high-performance liquid chromatography