title: DS6: anticandidal, antibiofilm peptide against Candida tropicalis and exhibit synergy with commercial drug.
abstract: Antifungal peptides have gained interest as therapeutic agents in recent years because of increased multidrug resistance against present antifungal drugs. This study designed, synthesized and characterized antifungal activity of a small peptide analogue, DS6. This peptide was designed using the template from the N-terminal part of the antifungal protein, Aspergillus giganteous. DS6 inhibited Candida tropicalis (ATCC 13803), as well as its clinical isolates. DS6 was found to be a fungicidal, killing the fungus very rapidly. DS6 is also non-toxic to human cells. Synergistic interactions of DS6 with amphotericin B and fluconazole were also evident. DS6 is membrane lytic and exhibits antibiofilm activity against C. tropicalis. In conclusion, DS6 may have utility as an alternative antifungal therapy for C. tropicalis. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd.
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, New Delhi, 110029, India.
authors.forename: Kusum
authors.initials: K
authors.lastname: Singh
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, New Delhi, 110029, India.
authors.forename: Shashank
authors.initials: S
authors.lastname: Shekhar
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, New Delhi, 110029, India.
authors.forename: Yudhishthir
authors.initials: Y
authors.lastname: Yadav
authors.affiliation: Microbiology, All India Institute of Medical Sciences, New Delhi, 110029, India.
authors.forename: Immaculata
authors.initials: I
authors.lastname: Xess
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, New Delhi, 110029, India.
authors.forename: Sharmistha
authors.initials: S
authors.lastname: Dey
mesh_terms: D020816:Amino Acid Motifs; D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002175:Candida / Q000187:drug effects / Q000254:growth & development; D041022:Candida tropicalis / Q000187:drug effects* / Q000254:growth & development; D002470:Cell Survival / Q000187:drug effects; D004338:Drug Combinations; D004357:Drug Synergism; D004912:Erythrocytes / Q000187:drug effects; D015725:Fluconazole / Q000494:pharmacology*; D057809:HEK293 Cells; D006801:Humans; D008826:Microbial Sensitivity Tests; D010455:Peptides / Q000138:chemical synthesis / Q000494:pharmacology*; D060327:Solid-Phase Synthesis Techniques / Q000379:methods; D013045:Species Specificity
chemical_list: D000935:Antifungal Agents; D004338:Drug Combinations; D010455:Peptides; D000666:Amphotericin B; D015725:Fluconazole
keywords: antimicrobial peptides; biofilm; cytotoxicity; non-albicans Candida; synergy