title: Research Advances and Detection Methodologies for Microbe-Derived Acetylcholinesterase Inhibitors: A Systemic Review.
abstract: Acetylcholinesterase inhibitors (AChEIs) are an attractive research subject owing to their potential applications in the treatment of neurodegenerative diseases. Fungi and bacteria are major producers of AChEIs. Their active ingredients of fermentation products include alkaloids, terpenoids, phenylpropanoids, and steroids. A variety of in vitro acetylcholinesterase inhibitor assays have been developed and used to measure the activity of acetylcholinesterases, including modified Ellman's method, thin layer chromatography bioautography, and the combined liquid chromatography-mass spectrometry/modified Ellman's method. In this review, we provide an overview of the different detection methodologies, the microbe-derived AChEIs, and their producing strains.
authors.affiliation: Fujian Key Laboratory of Innate Immune Biology, Fujian Normal University, Fuzhou 350117, China. sjq027@fjnu.edu.cn.|Biomedical Research Center of South China, Fujian Normal University, Fuzhou 350117, China. sjq027@fjnu.edu.cn.|College of Life Science, Fujian Normal University, Fuzhou 350117, China. sjq027@fjnu.edu.cn.
authors.forename: Jingqian
authors.initials: J
authors.lastname: Su
authors.affiliation: Fujian Key Laboratory of Innate Immune Biology, Fujian Normal University, Fuzhou 350117, China. liubrusc@163.com.|Biomedical Research Center of South China, Fujian Normal University, Fuzhou 350117, China. liubrusc@163.com.|College of Life Science, Fujian Normal University, Fuzhou 350117, China. liubrusc@163.com.
authors.forename: Huiying
authors.initials: H
authors.lastname: Liu
authors.affiliation: Fujian Key Laboratory of Innate Immune Biology, Fujian Normal University, Fuzhou 350117, China. gk9309@yeah.net.|Biomedical Research Center of South China, Fujian Normal University, Fuzhou 350117, China. gk9309@yeah.net.|College of Life Science, Fujian Normal University, Fuzhou 350117, China. gk9309@yeah.net.
authors.forename: Kai
authors.initials: K
authors.lastname: Guo
authors.affiliation: Tumor Invasion Microecological Laboratory, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, China. charmed1905@163.com.
authors.forename: Long
authors.initials: L
authors.lastname: Chen
authors.affiliation: College of Life Science, Fujian Normal University, Fuzhou 350117, China. minhe214@fjnu.edu.cn.
authors.forename: Minhe
authors.initials: M
authors.lastname: Yang
authors.affiliation: Fujian Key Laboratory of Innate Immune Biology, Fujian Normal University, Fuzhou 350117, China. nfsw@fjnu.edu.cn.|Biomedical Research Center of South China, Fujian Normal University, Fuzhou 350117, China. nfsw@fjnu.edu.cn.|College of Life Science, Fujian Normal University, Fuzhou 350117, China. nfsw@fjnu.edu.cn.
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000470:Alkaloids / Q000494:pharmacology; D000544:Alzheimer Disease / Q000188:drug therapy; D001419:Bacteria / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000032:analysis / Q000302:isolation & purification / Q000627:therapeutic use; D002853:Chromatography, Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D005658:Fungi / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods
chemical_list: D000110:Acetylcholinesterase; D000470:Alkaloids; D002800:Cholinesterase Inhibitors
keywords: Alzheimer’s disease; acetylcholinesterase inhibitors; in vitro assays