title: Analysis of genes involved in glycogen degradation in Escherichia coli.
abstract: Escherichia coli accumulate or degrade glycogen depending on environmental carbon supply. Glycogen phosphorylase (GlgP) and glycogen debranching enzyme (GlgX) are known to act on the glycogen polymer, while maltodextrin phosphorylase (MalP) is thought to remove maltodextrins released by GlgX. To examine the roles of these enzymes in more detail, single, double and triple mutants lacking all their activities were produced. GlgX and GlgP were shown to act directly on the glycogen polymer, while MalP most likely catabolised soluble malto-oligosaccharides. Interestingly, analysis of a triple mutant lacking all three enzymes indicates the presence of another enzyme that can release maltodextrins from glycogen.
authors.affiliation: Institute for Plant Biotechnology, Department of Genetics, University of Stellenbosch, Private Bag X1, Matieland 7602, Stellenbosch, South Africa.
authors.forename: Lindi
authors.initials: L
authors.lastname: Strydom
authors.affiliation: Institute for Plant Biotechnology, Department of Genetics, University of Stellenbosch, Private Bag X1, Matieland 7602, Stellenbosch, South Africa.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Jewell
authors.affiliation: Institute for Plant Biotechnology, Department of Genetics, University of Stellenbosch, Private Bag X1, Matieland 7602, Stellenbosch, South Africa.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Meier
authors.affiliation: Institute for Agricultural Sciences, ETH Zurich, 8092 Zurich, Switzerland.
authors.forename: Gavin M
authors.initials: GM
authors.lastname: George
authors.affiliation: Institute for Agricultural Sciences, ETH Zurich, 8092 Zurich, Switzerland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Pfister
authors.affiliation: Institute for Agricultural Sciences, ETH Zurich, 8092 Zurich, Switzerland.
authors.forename: Samuel
authors.initials: S
authors.lastname: Zeeman
authors.affiliation: Institute for Plant Biotechnology, Department of Genetics, University of Stellenbosch, Private Bag X1, Matieland 7602, Stellenbosch, South Africa.
authors.forename: Jens
authors.initials: J
authors.lastname: Kossmann
authors.affiliation: Institute for Plant Biotechnology, Department of Genetics, University of Stellenbosch, Private Bag X1, Matieland 7602, Stellenbosch, South Africa.
authors.forename: James R
authors.initials: JR
authors.lastname: Lloyd
mesh_terms: D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism*; D006004:Glycogen Debranching Enzyme System / Q000235:genetics; D024981:Glycogen Phosphorylase / Q000235:genetics / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D006004:Glycogen Debranching Enzyme System; D011134:Polysaccharides; C008315:maltodextrin; D006003:Glycogen; D005964:Glucosyltransferases; D024981:Glycogen Phosphorylase; C033938:maltodextrin phosphorylase
keywords: Escherichia coli; Glycogen; glycogen accumulation; glycogen debranching enzyme; glycogen phosphorylase; maltodextrin phosphorylase