title: Characterizing cell subsets using marker enrichment modeling.
abstract: Learning cell identity from high-content single-cell data presently relies on human experts. We present marker enrichment modeling (MEM), an algorithm that objectively describes cells by quantifying contextual feature enrichment and reporting a human- and machine-readable text label. MEM outperforms traditional metrics in describing immune and cancer cell subsets from fluorescence and mass cytometry. MEM provides a quantitative language to communicate characteristics of new and established cytotypes observed in complex tissues.
authors.affiliation: Department of Cancer Biology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.|Vanderbilt-Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
authors.forename: Kirsten E
authors.initials: KE
authors.lastname: Diggins
authors.affiliation: Vanderbilt-Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.|Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
authors.forename: Allison R
authors.initials: AR
authors.lastname: Greenplate
authors.affiliation: Department of Cancer Biology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.|Vanderbilt-Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
authors.forename: Nalin
authors.initials: N
authors.lastname: Leelatian
authors.affiliation: Vanderbilt-Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.|Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
authors.forename: Cara E
authors.identifier: 0000-0003-1797-7902
authors.initials: CE
authors.lastname: Wogsland
authors.affiliation: Department of Cancer Biology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.|Vanderbilt-Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.|Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
authors.forename: Jonathan M
authors.identifier: 0000-0001-9428-8866
authors.initials: JM
authors.lastname: Irish
mesh_terms: D000465:Algorithms; D015415:Biomarkers / Q000032:analysis; D001932:Brain Neoplasms / Q000276:immunology / Q000473:pathology*; D019295:Computational Biology / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D005909:Glioblastoma / Q000276:immunology / Q000473:pathology*; D006801:Humans; D059010:Single-Cell Analysis / Q000379:methods; D013601:T-Lymphocytes / Q000166:cytology
chemical_list: D015415:Biomarkers