title: Experimental studies on the inactivation of HBV in blood via riboflavin photochemical treatment.
abstract: The aim of the study was to examine the influence of riboflavin photochemical method on the inactivation of hepatitis B virus (HBV) and the functions of red blood cells. Twenty patients suffering viral hepatitis B were selected in this study, and venous blood was collected and final concentration of 1,500 µmol/l riboflavin were added, to accept the λ=400-500 nm. The light intensity of 40,000 lux was treated with 2 h. The effect of inactivation was then evaluated and the function of red blood cells was detected. Two hours after treatment of the blood samples with riboflavin (1,500 µmol/l), the numbers of copy of HBV DNA were significantly decreased (5.1×109±4.2×10 vs. 1.2×107±1.2×106after the inactivation, while, 2,3-DPG, Na+K+-ATPase, Ca2+-ATPase, Mg2+-ATPase, FHb were unchanged. In conclusion, HBV DNA can be reduced using riboflavin photochemical inactivation method. Inactivate the B virus had no effect on erythrocyte function.
authors.affiliation: Xuzhou Red Cross Blood Station, Xuzhou, Jiangsu 221006, P.R. China.
authors.forename: Zi-Yu
authors.initials: ZY
authors.lastname: Zhou
authors.affiliation: Xuzhou Red Cross Blood Station, Xuzhou, Jiangsu 221006, P.R. China.
authors.forename: Xing-Xiu
authors.initials: XX
authors.lastname: Bi
keywords: hepatitis B virus; photochemistry; red blood cell; riboflavin