title: Acetylation and deacetylation for sucralose preparation by a newly isolated Bacillus amyloliquefaciens WZS01.
abstract: Sucralose is a non-nutritive artificial sweetener used in a broad range of foods and beverages. In the present study, Bacillus amyloliquefaciens WZS01 was isolated, identified, and used as a catalyst both in regioselective acylation and deacetylation for sucralose preparation. Bacterial cells were immobilized on polyurethane foam and utilized to synthesize sucrose-6-acetate regioselectively. The yield of sucrose-6-acetate was >95% with 60 mM sucrose after 22 h of reaction. Free cells could hydrolyze 75 mM sucralose-6-acetate to produce sucralose with >99% yield after 24 h of reaction. B. amyloliquefaciens WZS01 could be considered a potential biocatalyst for sucralose preparation.
authors.affiliation: Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, PR China; Yi Fan Xin Fu Pharmaceutical Co., Ltd., No. 50 Qinshan road, Jincheng street, Lin'an, Zhejing 311300, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.affiliation: Yi Fan Xin Fu Pharmaceutical Co., Ltd., No. 50 Qinshan road, Jincheng street, Lin'an, Zhejing 311300, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.affiliation: Yi Fan Xin Fu Pharmaceutical Co., Ltd., No. 50 Qinshan road, Jincheng street, Lin'an, Zhejing 311300, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Lou
authors.affiliation: Yi Fan Xin Fu Pharmaceutical Co., Ltd., No. 50 Qinshan road, Jincheng street, Lin'an, Zhejing 311300, PR China.
authors.forename: Yanbing
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, PR China.
authors.forename: Xinjun
authors.initials: X
authors.lastname: Yu
authors.affiliation: Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, PR China; Yi Fan Xin Fu Pharmaceutical Co., Ltd., No. 50 Qinshan road, Jincheng street, Lin'an, Zhejing 311300, PR China.
authors.forename: Man
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, PR China. Electronic address: hzwangzhao@163.com.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000107:Acetylation; D000069977:Bacillus amyloliquefaciens / Q000302:isolation & purification* / Q000378:metabolism*; D055162:Biocatalysis; D018914:Cells, Immobilized / Q000378:metabolism; D011140:Polyurethanes; D013395:Sucrose / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D013549:Sweetening Agents / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D011140:Polyurethanes; D013549:Sweetening Agents; C556997:sucrose-6-acetate; D013395:Sucrose; C026285:trichlorosucrose
keywords: Acetylation; Bacillus amyloliquefaciens; Deacetylation; Sucralose; Sucrose-6-acetate