title: The effect of Echinococcus granulosus on spleen cells and TGF-β expression in the peripheral blood of BALB/c mice.
abstract: Cystic echinococcosis (CE) caused by the cestode Echinococcus granulosus (E. granulosus) is a zoonotic parasitic disease. The effective immune evasion mechanisms of E. granulosus allow it to parasitize its hosts. However, the status of the innate and adaptive immune cells and their contributions to E. granulosus progression remain poorly understood. In this study, we aimed to determine the impact of E. granulosus infection on T cells, NK cell responses and TGF-β expression during the early infection phase in BALB/c mice. In E. granulosus infections, there was an increasing tendency in the percentage of CD4+CD25+T cells and CD4+Foxp3+T cells and peripheral blood TGF-β levels and relative expression of the Foxp3 gene. Moreover, there were a decreasing tendency in the percentage of NK cells and NK cell cytotoxicity and the expression of NKG2D on NK cells. The TGF-β1/Smad pathway was activated by E. granulosus in mice. Above results can be reversed by the inhibitor SB-525334 (potent activin receptor-like kinase 5 inhibitor). These results suggest that the TGF-β/Smad pathway plays an important role in changes of T-cell or NK cell responses. These results may contribute to revealing the preliminary molecular mechanisms in establishing hydatid infection.
authors.affiliation: Department of Immunology, Shihezi University School of Medicine, Shihezi, Xinjiang, China.|College of One Health, Tongren University, Tongren, Guizhou, China.
authors.forename: S
authors.initials: S
authors.lastname: Yin
authors.affiliation: Department of Immunology, Shihezi University School of Medicine, Shihezi, Xinjiang, China.
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Agroforestry Engineering and Planning (Cultural and Technological Industry Innovation Research Center), Tongren University, Tongren, Guizhou, China.
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Immunology, Shihezi University School of Medicine, Shihezi, Xinjiang, China.
authors.forename: F
authors.initials: F
authors.lastname: Xu
authors.affiliation: Department of Immunology, Shihezi University School of Medicine, Shihezi, Xinjiang, China.
authors.forename: H
authors.initials: H
authors.lastname: Fang
authors.affiliation: Department of Immunology, Shihezi University School of Medicine, Shihezi, Xinjiang, China.
authors.forename: J
authors.initials: J
authors.lastname: Hou
authors.affiliation: Department of Immunology, Shihezi University School of Medicine, Shihezi, Xinjiang, China.
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang, China.
authors.forename: X
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Immunology, Shihezi University School of Medicine, Shihezi, Xinjiang, China.
authors.forename: X
authors.identifier: 0000-0002-8526-7198
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D004443:Echinococcosis / Q000097:blood / Q000276:immunology* / Q000473:pathology; D048209:Echinococcus granulosus / Q000276:immunology; D005260:Female; D007093:Imidazoles; D007694:Killer Cells, Natural; D051379:Mice; D008807:Mice, Inbred BALB C; D017346:Protein Serine-Threonine Kinases; D011810:Quinoxalines; D000077293:Receptor, Transforming Growth Factor-beta Type I; D018125:Receptors, Transforming Growth Factor beta; D013154:Spleen / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D016212:Transforming Growth Factor beta / Q000096:biosynthesis*
chemical_list: C521813:6-(2-tert-butyl-5-(6-methylpyridin-2-yl)-1H-imidazol-4-yl)quinoxaline; D007093:Imidazoles; D011810:Quinoxalines; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; D017346:Protein Serine-Threonine Kinases; D000077293:Receptor, Transforming Growth Factor-beta Type I
keywords:  Echinococcus granulosus ; CD4+CD25+ T cells; Foxp3 gene; TGF-β; flow cytometry