title: Alkaloids from the mangrove endophytic fungus Diaporthe phaseolorum SKS019.
abstract: Six new alkaloids including four new chromeno[3,2-c]pyridines, diaporphasines A-D (1-4), and two new isoindolinones, meyeroguillines C and D (6-7), as well as three known compounds meyeroguilline A (5), 5-deoxybostrycoidin (8), and fusaristatin A (9), were isolated from an endophytic fungus Diaporthe phaseolorum SKS019. Their structures were determined by analysis of 1D and 2D NMR and mass spectroscopic data. Compounds 1-9 are alkaloid components reported for the first time from the Diaporthe sp., and diaporphasines A-D (1-4) are the third examples of alkaloids possessing the unique chromeno[3,2-c]pyridine nucleus. All isolated compounds 1-9 were evaluated for their cytotoxic activity in vitro using MDA-MB-435, HepG2, MCF10A, HCT116, and NCI-H460 human cell lines. Compound 8 exhibited cytotoxicity against MDA-MB-435 and NCI-H460 human cancer cell lines with IC50values of 5.32 and 6.57μM, respectively, and compound 9 showed growth-inhibitory activity against MDA-MB-435 human cancer cell line with IC50value of 8.15μM.
authors.affiliation: School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Cui
authors.affiliation: Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Jianchen
authors.initials: J
authors.lastname: Yu
authors.affiliation: School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Senhua
authors.initials: S
authors.lastname: Chen
authors.affiliation: School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Ding
authors.affiliation: School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Xishan
authors.initials: X
authors.lastname: Huang
authors.affiliation: Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yuan
authors.affiliation: School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou 510006, China. Electronic address: cesshzhg@sysu.edu.cn.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: She
mesh_terms: D000470:Alkaloids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001203:Ascomycota / Q000737:chemistry* / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D045325:HCT116 Cells; D056945:Hep G2 Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation
chemical_list: D000470:Alkaloids
keywords: Alkaloid; Chromeno[3,2-c]pyridine; Cytotoxicity; Diaporthe phaseolorum; Isoindolinone