title: Peptide Paratope Mimics of the Broadly Neutralizing HIV-1 Antibody b12.
abstract: The broadly neutralizing HIV-1 antibody b12 recognizes the CD4 binding site of the HIV-1 envelope glycoprotein gp120 and efficiently neutralizes HIV-1 infections in vitro and in vivo. Based on the 3D structure of a b12⋅gp120 complex, we have designed an assembled peptide (b12-M) that presents the parts of the three heavy-chain complementarity-determining regions (CDRs) of b12, which contain the contact sites of the antibody for gp120. This b12-mimetic peptide, as well as a truncated peptide presenting only two of the three heavy-chain CDRs of b12, were shown to recognize gp120 in a similar manner to b12, as well as to inhibit HIV-1 infection, demonstrating functional mimicry of b12 by the paratope mimetic peptides.
authors.affiliation: Department of Chemistry and Pharmacy, University of Erlangen-Nürnberg, Schuhstrasse 19, 91052, Erlangen, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Haußner
authors.affiliation: Institute of Medical Microbiology and Hygiene, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053, Regensburg, Germany.
authors.forename: Dominik
authors.initials: D
authors.lastname: Damm
authors.affiliation: Institute of Medical Microbiology and Hygiene, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053, Regensburg, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Nirschl
authors.affiliation: Institute of Clinical Microbiology and Hygiene, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053, Regensburg, Germany.
authors.forename: Anette
authors.initials: A
authors.lastname: Rohrhofer
authors.affiliation: Institute of Medical Microbiology and Hygiene, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053, Regensburg, Germany.|Institute of Clinical Microbiology and Hygiene, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053, Regensburg, Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Schmidt
authors.affiliation: Department of Chemistry and Pharmacy, University of Erlangen-Nürnberg, Schuhstrasse 19, 91052, Erlangen, Germany.
authors.forename: Jutta
authors.initials: J
authors.lastname: Eichler
mesh_terms: D019380:Anti-HIV Agents / Q000138:chemical synthesis / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D057134:Antibodies, Neutralizing / Q000276:immunology*; D001665:Binding Sites; D002460:Cell Line; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000276:immunology; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D015202:Protein Engineering
chemical_list: D019380:Anti-HIV Agents; D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D015699:HIV Envelope Protein gp120; D007143:Immunoglobulin Heavy Chains; D010455:Peptides
keywords: HIV-1; MAb b12; antibody mimics; gp120; immunochemistry; peptides