title: The ability of lipopolysaccharide (LPS) of Pasteurella multocida B:2 to induce clinical and pathological lesions in the nervous system of buffalo calves following experimental inoculation.
abstract: Lipopolysaccharide (LPS) of P. multocida B:2, a causative agent of haemorrhagic septicaemia (HS) in cattle and buffaloes, is considered as the main virulence factor and contribute in the pathogenesis of the disease. Recent studies provided evidences about the involvement of the nervous system in pathogenesis of HS. However, the role of P. multocida B:2 immunogens, especially the LPS is still uncovered. Therefore, this study was designed to investigate the role of P. multocida B:2 LPS to induce pathological changes in the nervous system. Nine eight-month-old, clinically healthy buffalo calves were used and distributed into three groups. Calves of Group 1 and 2 were inoculated orally and intravenously with 10 ml of LPS broth extract represent 1 × 1012cfu/ml of P. multocida B:2, respectively, while calves of Group 3 were inoculated orally with 10 ml of phosphate buffer saline as a control. Significant differences were found in the mean scores for clinical signs, post mortem and histopathological changes especially in Group 2, which mainly affect different anatomic regions of the nervous system, mainly the brain. On the other hand, lower scores have been recorded for clinical signs, gross and histopathological changes in Group 1. These results provide for the first time strong evidence about the ability of P. multocida B:2 LPS to cross the blood brain barrier and induce pathological changes in the nervous system of the affected buffalo calves.
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Malaysia; Department of Veterinary Internal Medicine, College of Veterinary Medicine, Al-Qasim Green University, Iraq.
authors.forename: Ali Dhiaa
authors.initials: AD
authors.lastname: Marza
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Malaysia; Research Centre for Ruminant Diseases, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Malaysia. Electronic address: jesseariasamy@gmail.com.
authors.forename: Faez Firdaus
authors.initials: FF
authors.lastname: Jesse Abdullah
authors.affiliation: Department of Microbiology, College of Veterinary Medicine, University of Mosul, Iraq.
authors.forename: Ihsan Muneer
authors.initials: IM
authors.lastname: Ahmed
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Malaysia.
authors.forename: Eric Lim
authors.initials: EL
authors.lastname: Teik Chung
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Malaysia; Babil Technical Institute, Al Furat Al-Awsat Technical University, Iraq.
authors.forename: Hayder Hamzah
authors.initials: HH
authors.lastname: Ibrahim
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Malaysia.
authors.forename: Mohd
authors.initials: M
authors.lastname: Zamri-Saad
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Malaysia.
authors.forename: Abdul Rahman
authors.initials: AR
authors.lastname: Omar
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Malaysia.
authors.forename: Md Zuki
authors.initials: MZ
authors.lastname: Abu Bakar
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Malaysia.
authors.forename: Abdul Aziz
authors.initials: AA
authors.lastname: Saharee
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Malaysia.
authors.forename: Abdul Wahid
authors.initials: AW
authors.lastname: Haron
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, University of Baghdad, Iraq.
authors.forename: Mohammed Jwaid
authors.initials: MJ
authors.lastname: Alwan
authors.affiliation: Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Malaysia.
authors.forename: Mohd Azmi
authors.initials: MA
authors.lastname: Mohd Lila
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology; D002020:Buffaloes; D006483:Hemorrhagic Septicemia / Q000382:microbiology* / Q000473:pathology; D006651:Histocytochemistry; D008070:Lipopolysaccharides / Q000302:isolation & purification / Q000633:toxicity*; D008853:Microscopy; D009420:Nervous System / Q000382:microbiology* / Q000473:pathology; D016979:Pasteurella multocida / Q000737:chemistry*; D011041:Poisoning / Q000473:pathology*; D013116:Spinal Cord / Q000473:pathology
chemical_list: D008070:Lipopolysaccharides
keywords: Buffalo calves; Clinical; Haemorrhagic septicaemia; Lipopolysaccharide (LPS); Nervous system; Pasteurella multocida B:2; Pathological