title: SINE polymorphism reveals distinct strains of Entamoeba histolytica from North India.
abstract: Entamoeba histolytica is an intestinal parasite causing significant morbidity and mortality in the developing world. More tools are needed to understand the epidemiology and molecular pathogenesis of amebiasis. Virulence pattern of E. histolytica could be linked with the genotype of a strain. Several loci showing insertion polymorphism of retrotransposable short interspersed nuclear elements EhSINE1 and EhSINE2 have been reported among laboratory strains of E. histolytica. The present study was undertaken to validate this observation in clinical isolates from north India. Our results indicate that the Indian samples show a different propensity of SINE retention or loss at two of the polymorphic loci compared with non-Indian samples. Statistical analysis of different loci revealed Locus 17 of EhSINE1as a potential geographical marker for distinguishing Indian isolates from non Indian isolates.
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India.
authors.forename: Shraddha
authors.initials: S
authors.lastname: Sharma
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India.
authors.forename: Neha
authors.initials: N
authors.lastname: Banyal
authors.affiliation: Department of Pathology, Safdarjung Hospital, New Delhi, India.
authors.forename: Mukul
authors.initials: M
authors.lastname: Singh
authors.affiliation: Department of Pathology, Safdarjung Hospital, New Delhi, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Mandal
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India.
authors.forename: Sudha
authors.initials: S
authors.lastname: Bhattacharya
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, New Delhi, India. Electronic address: jpaul33@hotmail.com.
authors.forename: Jaishree
authors.initials: J
authors.lastname: Paul
mesh_terms: D015139:Blotting, Southern; D004251:DNA Transposable Elements; D016054:DNA, Protozoan / Q000737:chemistry* / Q000302:isolation & purification; D004748:Entamoeba histolytica / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D004749:Entamoebiasis / Q000453:epidemiology / Q000469:parasitology*; D005243:Feces / Q000469:parasitology; D005838:Genotype; D006801:Humans; D015151:Immunoblotting; D007194:India / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D020086:Short Interspersed Nucleotide Elements / Q000235:genetics*; D014774:Virulence
chemical_list: D004251:DNA Transposable Elements; D016054:DNA, Protozoan
keywords: Clinical isolates; EhSINE; Entamoeba histolytica; Geographical marker; SINE polymorphism; Strain typing