title: General Toxicity and Antifungal Activity of a New Dental Gel with Essential Oil from Abies Sibirica L.
abstract: BACKGROUND The aim of this study was to analyze the antifungal activity and the general toxicity of a new dental gel containing essential oil from the tree Abies sibirica L., which grows in the Republic of Kazakhstan. MATERIAL AND METHODS The essential oil from Abies sibirica L. was obtained by microwave heating method using the STARTE Microwave Extraction System. Adjutants used to prepare the oil were carbomer 974P, glycerin, polysorbate 80, xylitol, triethanolamine, and purified water, all allowed for medical usage. The antifungal activity of the essential oil was assessed by monitoring the optical density of Candida albicans in a microplate reader. The safety was determined by analyzing the acute and subacute toxicity. RESULTS The essential oil obtained by the microwave heating method revealed a higher antifungal activity in comparison with the essential oil obtained by the steam distillation method. No obvious changes were detected in guinea pigs following cutaneous application of the gel. Enteral administration of the essential oil caused minimal functional and histological changes in mice after 4 weeks. The new harmless dental gel containing pine oil from Abies sibirica L. was provided for the purposes of this particular clinical research. CONCLUSIONS The high antifungal activity of the gel is the basis for more in-depth studies on its safety and pharmacological activity.
authors.affiliation: Institute of Physiology and Pharmacology, Lithuanian University of Health Sciences, Kaunas, Lithuania.
authors.forename: Aurelija
authors.initials: A
authors.lastname: Noreikaitė
authors.affiliation: Department of Pharmacist-Technologist, Asfendiyarov Kazakh National Medical University, Almaty, Kazakhstan.
authors.forename: Rizvangul
authors.initials: R
authors.lastname: Ayupova
authors.affiliation: Department of Pharmacology, Asfendiyarov Kazakh National Medical University, Almaty, Kazakhstan.
authors.forename: Elmira
authors.initials: E
authors.lastname: Satbayeva
authors.affiliation: Department of Pharmacology, Asfendiyarov Kazakh National Medical University, Almaty, Kazakhstan.
authors.forename: Aida
authors.initials: A
authors.lastname: Seitaliyeva
authors.affiliation: Department of Pharmacology, Asfendiyarov Kazakh National Medical University, Almaty, Kazakhstan.
authors.forename: Marzhan
authors.initials: M
authors.lastname: Amirkulova
authors.affiliation: Department of Pharmacology, Asfendiyarov Kazakh National Medical University, Almaty, Kazakhstan.
authors.forename: Guram
authors.initials: G
authors.lastname: Pichkhadze
authors.affiliation: Institute of Pharmacy, Asfendiyarov Kazakh National Medical University, Almaty, Kazakhstan.
authors.forename: Ubaidilla
authors.initials: U
authors.lastname: Datkhayev
authors.affiliation: Institute of Physiology and Pharmacology, Lithuanian University of Health Sciences, Kaunas, Lithuania.
authors.forename: Edgaras
authors.initials: E
authors.lastname: Stankevičius
mesh_terms: D028202:Abies / Q000737:chemistry*; D000278:Administration, Buccal; D000818:Animals; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D002176:Candida albicans / Q000187:drug effects; D003764:Dental Materials / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity; D005782:Gels / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity; D006168:Guinea Pigs; D051379:Mice; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D011817:Rabbits
chemical_list: D000935:Antifungal Agents; D003764:Dental Materials; D005782:Gels; D009822:Oils, Volatile