title: A phase II trial of stereotactic body radiotherapy with concurrent anti-PD1 treatment in metastatic melanoma: evaluation of clinical and immunologic response.
abstract: Antibodies blocking programmed cell death 1 (PD-1) have encouraging responses in patients with metastatic melanoma. Response to anti-PD-1 treatment requires pre-existing CD8+ T cells that are negatively regulated by PD-1-mediated adaptive immune resistance. Unfortunately, less than half of melanoma tumours have these characteristics. Combining anti-PD-1 treatment with other immunomodulating treatments to activate CD8+ T cells is therefore of vital importance to increase response rates and long-term survival benefit in melanoma patients. Both preclinical and retrospective clinical data support the hypothesis that radiotherapy increases the response rates to anti-PD-1 treatment by stimulating the accumulation and activation of CD8+ T cells in the tumour microenvironment. Combining radiotherapy with a PD-1 blocking antibody might therefore increase response rates and even induce long-term survival. The current phase II study will be testing these hypotheses and aims to improve local and distant tumour responses by exploiting the pro-immunogenic effects of radiotherapy in addition to anti-PD-1 treatment.
  
  
  
  The trial will be conducted in patients with metastatic melanoma. Nivolumab or pembrolizumab, both antibodies that target PD-1, will be administrated according to the recommended dosing schedule. Prior to the 2nd cycle, radiotherapy will be delivered in three fractions of 8 Gy to the largest FDG-avid metastatic lesion. The primary endpoint is the proportion of patients with a partial or complete response in non-irradiated metastases according to RECIST v1.1. Secondary endpoints include response rate according to immune related response criteria, metabolic response, local control and survival. To identify peripheral blood biomarkers, peripheral blood mononuclear cells and serum samples will be collected prospectively before, during and after treatment and subjected to flow cytometry and cytokine measurement.
  
  
  
  The current phase II trial aims at exploring the suggested benefits of combining anti-PD-1 treatment and radiotherapy. The translational focus on immunologic markers might be suitable for predicting efficacy and monitoring the effect so to improve patient selection for future clinical applications. ClinicalTrials.gov Identifier NCT02821182.
authors.affiliation: Department of Radiation-Oncology, University Hospital Ghent, De pintelaan 185, 9000, Ghent, Belgium. katrien.dewolf@ugent.be.
authors.forename: Katrien
authors.initials: K
authors.lastname: De Wolf
authors.affiliation: Department of Medical Oncology, University Hospital Ghent, Ghent, Belgium.
authors.forename: Vibeke
authors.initials: V
authors.lastname: Kruse
authors.affiliation: Department of Radiation-Oncology, University Hospital Ghent, De pintelaan 185, 9000, Ghent, Belgium.
authors.forename: Nora
authors.initials: N
authors.lastname: Sundahl
authors.affiliation: Department of Dermatology, University Hospital Ghent, Ghent, Belgium.
authors.forename: Mireille
authors.initials: M
authors.lastname: van Gele
authors.affiliation: Department of Dermatology, University Hospital Ghent, Ghent, Belgium.
authors.forename: Ines
authors.initials: I
authors.lastname: Chevolet
authors.affiliation: Department of Dermatology, University Hospital Ghent, Ghent, Belgium.
authors.forename: Reinhart
authors.initials: R
authors.lastname: Speeckaert
authors.affiliation: Department of Dermatology, University Hospital Ghent, Ghent, Belgium.
authors.forename: Lieve
authors.initials: L
authors.lastname: Brochez
authors.affiliation: Department of Radiation-Oncology, University Hospital Ghent, De pintelaan 185, 9000, Ghent, Belgium.
authors.forename: Piet
authors.initials: P
authors.lastname: Ost
mesh_terms: D017322:Clinical Trials, Phase II as Topic; D023381:Endpoint Determination; D005500:Follow-Up Studies; D006801:Humans; D008545:Melanoma / Q000276:immunology / Q000556:secondary* / Q000628:therapy*; D017063:Outcome Assessment, Health Care; D061026:Programmed Cell Death 1 Receptor / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016634:Radiosurgery; D018401:Sample Size; D016896:Treatment Outcome
chemical_list: D061026:Programmed Cell Death 1 Receptor
keywords: Biomarkers; Cancer immunotherapy; Immune monitoring; Metastatic melanoma; Stereotactic body radiotherapy