title: Validation of a rapid DNA process with the RapidHIT®ID system using GlobalFiler®Express chemistry, a platform optimized for decentralized testing environments.
abstract: The RapidHIT®ID is a fully automated sample-to-answer system for short tandem repeat (STR)-based human identification. The RapidHIT ID has been optimized for use in decentralized environments and processes presumed single source DNA samples, generating Combined DNA Index System (CODIS)-compatible DNA profiles in less than 90min. The system is easy to use, requiring less than one minute of hands-on time. Profiles are reviewed using centralized linking software, RapidLINK™ (IntegenX, Pleasanton, CA), a software tool designed to collate DNA profiles from single or multiple RapidHIT ID systems at different geographic locations. The RapidHIT ID has been designed to employ GlobalFiler®Express and AmpFLSTR®NGMSElect™, Thermo Fisher Scientific (Waltham, MA) STR chemistries. The Developmental Validation studies were performed using GlobalFiler®Express with single source reference samples according to Scientific Working Group for DNA Analysis Methods guidelines. These results show that multiple RapidHIT ID systems networked with RapidLINK software form a highly reliable system for wide-scale deployment in locations such as police booking stations and border crossings enabling real-time testing of arrestees, potential human trafficking victims, and other instances where rapid turnaround is essential.
authors.affiliation: IntegenX Inc, 5720 Stoneridge Drive, Suite 300, Pleasanton, CA, 94588, USA.
authors.forename: Susana
authors.initials: S
authors.lastname: Salceda
authors.affiliation: IntegenX Inc, 5720 Stoneridge Drive, Suite 300, Pleasanton, CA, 94588, USA.
authors.forename: Arnaldo
authors.initials: A
authors.lastname: Barican
authors.affiliation: IntegenX Inc, 5720 Stoneridge Drive, Suite 300, Pleasanton, CA, 94588, USA.
authors.forename: Jacklyn
authors.initials: J
authors.lastname: Buscaino
authors.affiliation: IntegenX Inc, 5720 Stoneridge Drive, Suite 300, Pleasanton, CA, 94588, USA.
authors.forename: Bruce
authors.initials: B
authors.lastname: Goldman
authors.affiliation: IntegenX Inc, 5720 Stoneridge Drive, Suite 300, Pleasanton, CA, 94588, USA.
authors.forename: Jim
authors.initials: J
authors.lastname: Klevenberg
authors.affiliation: IntegenX Inc, 5720 Stoneridge Drive, Suite 300, Pleasanton, CA, 94588, USA.
authors.forename: Melissa
authors.initials: M
authors.lastname: Kuhn
authors.affiliation: IntegenX Inc, 5720 Stoneridge Drive, Suite 300, Pleasanton, CA, 94588, USA.
authors.forename: Dennis
authors.initials: D
authors.lastname: Lehto
authors.affiliation: IntegenX Inc, 5720 Stoneridge Drive, Suite 300, Pleasanton, CA, 94588, USA.
authors.forename: Frank
authors.initials: F
authors.lastname: Lin
authors.affiliation: IntegenX Inc, 5720 Stoneridge Drive, Suite 300, Pleasanton, CA, 94588, USA.
authors.forename: Phong
authors.initials: P
authors.lastname: Nguyen
authors.affiliation: IntegenX Inc, 5720 Stoneridge Drive, Suite 300, Pleasanton, CA, 94588, USA.
authors.forename: Charles
authors.initials: C
authors.lastname: Park
authors.affiliation: IntegenX Inc, 5720 Stoneridge Drive, Suite 300, Pleasanton, CA, 94588, USA.
authors.forename: Francesca
authors.initials: F
authors.lastname: Pearson
authors.affiliation: IntegenX Inc, 5720 Stoneridge Drive, Suite 300, Pleasanton, CA, 94588, USA.
authors.forename: Rick
authors.initials: R
authors.lastname: Pittaro
authors.affiliation: IntegenX Inc, 5720 Stoneridge Drive, Suite 300, Pleasanton, CA, 94588, USA.
authors.forename: Sayali
authors.initials: S
authors.lastname: Salodkar
authors.affiliation: IntegenX Inc, 5720 Stoneridge Drive, Suite 300, Pleasanton, CA, 94588, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Schueren
authors.affiliation: IntegenX Inc, 5720 Stoneridge Drive, Suite 300, Pleasanton, CA, 94588, USA.
authors.forename: Corey
authors.initials: C
authors.lastname: Smith
authors.affiliation: IntegenX Inc, 5720 Stoneridge Drive, Suite 300, Pleasanton, CA, 94588, USA.
authors.forename: Charles
authors.initials: C
authors.lastname: Troup
authors.affiliation: IntegenX Inc, 5720 Stoneridge Drive, Suite 300, Pleasanton, CA, 94588, USA.
authors.forename: Dean
authors.initials: D
authors.lastname: Tsou
authors.affiliation: IntegenX Inc, 5720 Stoneridge Drive, Suite 300, Pleasanton, CA, 94588, USA.
authors.forename: Mattias
authors.initials: M
authors.lastname: Vangbo
authors.affiliation: IntegenX Inc, 5720 Stoneridge Drive, Suite 300, Pleasanton, CA, 94588, USA.
authors.forename: Justus
authors.initials: J
authors.lastname: Wunderle
authors.affiliation: IntegenX Inc, 5720 Stoneridge Drive, Suite 300, Pleasanton, CA, 94588, USA. Electronic address: davidk@integenx.com.
authors.forename: David
authors.initials: D
authors.lastname: King
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics*; D016172:DNA Fingerprinting; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation*; D006801:Humans; D018895:Microsatellite Repeats; D015203:Reproducibility of Results; D012984:Software; D013045:Species Specificity
chemical_list: D004247:DNA
keywords: GlobalFiler(®) Express; Presumed single source sample; Rapid DNA RapidHIT(®); RapidHIT(®) ID; RapidLINK™; STR; SWGDAM developmental validation