title: Identification of three immunodominant motifs with atypical isotype profile scattered over the Onchocerca volvulus proteome.
abstract: Understanding the immune response upon infection with the filarial nematode Onchocerca volvulus and the mechanisms that evolved in this parasite to evade immune mediated elimination is essential to expand the toolbox available for diagnostics, therapeutics and vaccines development. Using high-density peptide microarrays we scanned the proteome-wide linear epitope repertoire in Cameroonian onchocerciasis patients and healthy controls from Southern Africa which led to the identification of 249 immunodominant antigenic peptides. Motif analysis learned that 3 immunodominant motifs, encompassing 3 linear epitopes, are present in 70, 43, and 31 of these peptides, respectively and appear to be scattered over the entire proteome in seemingly non-related proteins. These linear epitopes are shown to have an atypical isotype profile dominated by IgG1, IgG3, IgE and IgM, in contrast to the commonly observed IgG4 response in chronic active helminth infections. The identification of these linear epitope motifs may lead to novel diagnostic development but further evaluation of cross-reactivity against common co-infecting human nematode infections will be needed.
authors.affiliation: Janssen Diagnostics, Janssen Pharmaceutica NV, Beerse, Belgium.
authors.forename: Ole
authors.identifier: 0000-0002-0638-1313
authors.initials: O
authors.lastname: Lagatie
authors.affiliation: Janssen Diagnostics, Janssen Pharmaceutica NV, Beerse, Belgium.
authors.forename: Bieke
authors.initials: B
authors.lastname: Van Dorst
authors.affiliation: Janssen Diagnostics, Janssen Pharmaceutica NV, Beerse, Belgium.
authors.forename: Lieven J
authors.initials: LJ
authors.lastname: Stuyver
mesh_terms: D000293:Adolescent; D000328:Adult; D000353:Africa, Southern; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D016022:Case-Control Studies; D003429:Cross Reactions; D018604:Epitope Mapping; D005260:Female; D006801:Humans; D016056:Immunodominant Epitopes / Q000276:immunology*; D007132:Immunoglobulin Isotypes / Q000097:blood; D008297:Male; D008875:Middle Aged; D017181:Onchocerca volvulus / Q000276:immunology*; D009855:Onchocerciasis / Q000276:immunology*; D040081:Protein Array Analysis; D054730:Protein Interaction Domains and Motifs / Q000276:immunology*; D020543:Proteome; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D000909:Antibodies, Helminth; D016056:Immunodominant Epitopes; D007132:Immunoglobulin Isotypes; D020543:Proteome