title: Identification of BALB/c Immune Markers Correlated with a Partial Protection to Leishmania infantum after Vaccination with a Rationally Designed Multi-epitope Cysteine Protease A Peptide-Based Nanovaccine.
abstract: Through their increased potential to be engaged and processed by dendritic cells (DCs), nanovaccines consisting of Poly(D,L-lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) loaded with both antigenic moieties and adjuvants are attractive candidates for triggering specific defense mechanisms against intracellular pathogens. The aim of the present study was to evaluate the immunogenicity and prophylactic potential of a rationally designed multi-epitope peptide of Leishmania Cysteine Protease A (CPA160-189) co-encapsulated with Monophosphoryl lipid A (MPLA) in PLGA NPs against L. infantum in BALB/c mice and identify immune markers correlated with protective responses.
  
  
  
  The DCs phenotypic and functional features exposed to soluble (CPA160-189, CPA160-189+MPLA) or encapsulated in PLGA NPs forms of peptide and adjuvant (PLGA-MPLA, PLGA-CPA160-189, PLGA-CPA160-189+MPLA) was firstly determined using BALB/c bone marrow-derived DCs. The most potent signatures of DCs maturation were obtained with the PLGA-CPA160-189+MPLA NPs. Subcutaneous administration of PLGA-CPA160-189+MPLA NPs in BALB/c mice induced specific anti-CPA160-189 cellular and humoral immune responses characterized by T cells producing high amounts of IL-2, IFN-γ and TNFα and IgG1/IgG2a antibodies. When these mice were challenged with 2x107 stationary phase L. infantum promastigotes, they displayed significant reduced hepatic (48%) and splenic (90%) parasite load at 1 month post-challenge. This protective phenotype was accompanied by a strong spleen lymphoproliferative response and high levels of IL-2, IFN-γ and TNFα versus low IL-4 and IL-10 secretion. Although, at 4 months post-challenge, the reduced parasite load was preserved in the liver (61%), an increase was detected in the spleen (30%), indicating a partial vaccine-induced protection.
  
  
  
  This study provide a basis for the development of peptide-based nanovaccines against leishmaniasis, since it reveals that vaccination with well-defined Leishmania MHC-restricted epitopes extracted from various immunogenic proteins co-encapsulated with the proper adjuvant or/and phlebotomine fly saliva multi-epitope peptides into clinically compatible PLGA NPs could be a promising approach for the induction of a strong and sustainable protective immunity.
authors.affiliation: Department of Microbiology, Hellenic Pasteur Institute, Athens, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Agallou
authors.affiliation: Department of Microbiology, Hellenic Pasteur Institute, Athens, Greece.
authors.forename: Maritsa
authors.initials: M
authors.lastname: Margaroni
authors.affiliation: Department of Microbiology, Hellenic Pasteur Institute, Athens, Greece.
authors.forename: Evita
authors.initials: E
authors.lastname: Athanasiou
authors.affiliation: Department of Microbiology, Hellenic Pasteur Institute, Athens, Greece.
authors.forename: Dimitra K
authors.initials: DK
authors.lastname: Toubanaki
authors.affiliation: Chemical Process & Energy Resources Institute, Centre for Research and Technology Hellas, Thessaloniki, Greece.
authors.forename: Katerina
authors.initials: K
authors.lastname: Kontonikola
authors.affiliation: Chemical Process & Energy Resources Institute, Centre for Research and Technology Hellas, Thessaloniki, Greece.
authors.forename: Konstantina
authors.initials: K
authors.lastname: Karidi
authors.affiliation: Chemical Process & Energy Resources Institute, Centre for Research and Technology Hellas, Thessaloniki, Greece.
authors.forename: Olga
authors.initials: O
authors.lastname: Kammona
authors.affiliation: Chemical Process & Energy Resources Institute, Centre for Research and Technology Hellas, Thessaloniki, Greece.|Department of Chemical Engineering, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors.forename: Costas
authors.initials: C
authors.lastname: Kiparissides
authors.affiliation: Department of Microbiology, Hellenic Pasteur Institute, Athens, Greece.
authors.forename: Evdokia
authors.identifier: 0000-0002-0987-4877
authors.initials: E
authors.lastname: Karagouni
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D057056:Cysteine Proteases / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology; D000939:Epitopes / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D018314:Leishmania infantum / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D054332:Leishmaniasis Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D015800:Protozoan Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014611:Vaccination; D014612:Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D000939:Epitopes; D007376:Interleukin-2; D054332:Leishmaniasis Vaccines; D015800:Protozoan Proteins; D014612:Vaccines; D015847:Interleukin-4; D057056:Cysteine Proteases