title: Tetrahedral DNA Nanostructure: A Potential Promoter for Cartilage Tissue Regeneration via Regulating Chondrocyte Phenotype and Proliferation.
abstract: Utilizing biomaterials to regulate the phenotype and proliferation of chondrocytes is a promising approach for effective cartilage tissue regeneration. Recently, a significant amount of effort has been invested into directing chondrocytes toward a desired location and function by utilizing biomaterials to control the dedifferentiation and phenotypic loss of chondrocytes during in vitro monolayer culture. Here, the transmission signals resulting from tetrahedral DNA nanostructures (TDNs) in the regulation of chondrocyte phenotype and proliferation are exploited. TDNs, new DNA nanomaterials, have been considered as promising materials in biomedical fields. Upon exposure to TDNs, chondrocyte phenotype is significantly enhanced, accompanied by lower gene expression related to Notch signaling pathway and higher expression of type II collagen. In addition, the cell proliferation and morphology of chondrocytes are changed after exposure to TDNs. In conclusion, this work demonstrates that TDNs are potentially useful mechanism in cartilage tissue regeneration from chondrocytes, whereby chondrocyte phenotype and proliferation can be retained.
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, P. R. China.
authors.forename: Xiaoru
authors.initials: X
authors.lastname: Shao
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, P. R. China.
authors.forename: Shiyu
authors.initials: S
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, P. R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, P. R. China.
authors.forename: Sirong
authors.initials: S
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, P. R. China.
authors.forename: Xueqin
authors.initials: X
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, P. R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, P. R. China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Lin
mesh_terms: D053674:Aggrecans / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002356:Cartilage / Q000502:physiology*; D049109:Cell Proliferation; D048430:Cell Shape / Q000235:genetics; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D004247:DNA / Q000737:chemistry*; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D049329:Nanostructures / Q000737:chemistry*; D010641:Phenotype; D051381:Rats; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism; D012038:Regeneration / Q000502:physiology*; D015854:Up-Regulation; D060449:Wnt Signaling Pathway / Q000235:genetics
chemical_list: D053674:Aggrecans; D024043:Collagen Type II; D051880:Receptors, Notch; D004247:DNA
keywords: chondrocytes; nanomaterials; notch signaling pathway; self-assembly; tetrahedral DNA nanostructures