title: Analysis of the Aldosterone Synthase (CYP11B2) and 11β-Hydroxylase (CYP11B1) Genes.
abstract: The aldosterone synthase (CYP11B2) and 11β-hydroxylase (CYP11B1) enzymes are known to be important players in the development of hypertension. Sequencing of the CYP11B2 and CYP11B1 genes and quantification of their respective mRNAs is greatly complicated by their high degree of sequence similarity. The need to ensure gene specificity during such analysis has required the development of particular methods for the detection of key polymorphisms at these loci, which are detailed in this chapter.
authors.affiliation: Institute of Cardiovascular and Medical Sciences, BHF Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, UK.
authors.forename: Scott M
authors.initials: SM
authors.lastname: MacKenzie
authors.affiliation: Institute of Cardiovascular and Medical Sciences, BHF Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, UK. Eleanor.Davies@glasgow.ac.uk.
authors.forename: Eleanor
authors.initials: E
authors.lastname: Davies
authors.affiliation: Institute of Cardiovascular and Medical Sciences, BHF Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, UK.
authors.forename: Samantha
authors.initials: S
authors.lastname: Alvarez-Madrazo
mesh_terms: D000818:Animals; D019405:Cytochrome P-450 CYP11B2 / Q000235:genetics*; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D011110:Polymorphism, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013252:Steroid 11-beta-Hydroxylase / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D019405:Cytochrome P-450 CYP11B2; D013252:Steroid 11-beta-Hydroxylase
keywords: 11β-Hydroxylase; Aldosterone synthase; CYP11B1; CYP11B2; Genotyping; PCR; Polymorphisms; RT-PCR