title: Two years of surveillance of influenza a virus infection in a swine herd. Results of virological, serological and pathological studies.
abstract: Swine farms provide a dynamic environment for the evolution of influenza A viruses (IAVs). The present report shows the results of a surveillance effort of IAV infection in one commercial swine farm in Argentina. Two cross-sectional serological and virological studies (n=480) were carried out in 2011 and 2012. Virus shedding was detected in nasal samples from pigs from ages 7, 21 and 42-days old. More than 90% of sows and gilts but less than 40% of 21-days old piglets had antibodies against IAV. In addition, IAV was detected in 8/17 nasal swabs and 10/15 lung samples taken from necropsied pigs. A subset of these samples was further processed for virus isolation resulting in 6 viruses of the H1N2 subtype (δ2 cluster). Pathological studies revealed an association between suppurative bronchopneumonia and necrotizing bronchiolitis with IAV positive samples. Statistical analyses showed that the degree of lesions in bronchi, bronchiole, and alveoli was higher in lungs positive to IAV. The results of this study depict the relevance of continuing long-term active surveillance of IAV in swine populations to establish IAV evolution relevant to swine and humans.
authors.affiliation: Instituto de Virología, CICVyA, INTA, Nicolas Repetto y de los Reseros s/n, 1686, Hurlingham, Buenos Aires, Argentina; CONICET, Av. Rivadavia 1917, C1033AAJ, CABA, Argentina. Electronic address: cappuccio.javier@inta.gob.ar.
authors.forename: Javier
authors.initials: J
authors.lastname: Cappuccio
authors.affiliation: Instituto de Virología, CICVyA, INTA, Nicolas Repetto y de los Reseros s/n, 1686, Hurlingham, Buenos Aires, Argentina; CONICET, Av. Rivadavia 1917, C1033AAJ, CABA, Argentina.
authors.forename: Marina
authors.initials: M
authors.lastname: Dibarbora
authors.affiliation: CONICET, Av. Rivadavia 1917, C1033AAJ, CABA, Argentina; Laboratorio de Patología Especial Veterinaria, Facultad de Ciencias Veterinarias, UNLP. 60 y 118 s/n, 1900, La Plata, Buenos Aires, Argentina.
authors.forename: Inés
authors.initials: I
authors.lastname: Lozada
authors.affiliation: Laboratorio de Patología Especial Veterinaria, Facultad de Ciencias Veterinarias, UNLP. 60 y 118 s/n, 1900, La Plata, Buenos Aires, Argentina.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Quiroga
authors.affiliation: Instituto de Virología, CICVyA, INTA, Nicolas Repetto y de los Reseros s/n, 1686, Hurlingham, Buenos Aires, Argentina.
authors.forename: Valeria
authors.initials: V
authors.lastname: Olivera
authors.affiliation: Instituto de Virología, CICVyA, INTA, Nicolas Repetto y de los Reseros s/n, 1686, Hurlingham, Buenos Aires, Argentina.
authors.forename: Marta
authors.initials: M
authors.lastname: Dángelo
authors.affiliation: CONICET, Av. Rivadavia 1917, C1033AAJ, CABA, Argentina; Laboratorio de Patología Especial Veterinaria, Facultad de Ciencias Veterinarias, UNLP. 60 y 118 s/n, 1900, La Plata, Buenos Aires, Argentina.
authors.forename: Estefanía
authors.initials: E
authors.lastname: Pérez
authors.affiliation: Laboratorio de Patología Especial Veterinaria, Facultad de Ciencias Veterinarias, UNLP. 60 y 118 s/n, 1900, La Plata, Buenos Aires, Argentina.
authors.forename: Hernán
authors.initials: H
authors.lastname: Barrales
authors.affiliation: Laboratorio de Patología Especial Veterinaria, Facultad de Ciencias Veterinarias, UNLP. 60 y 118 s/n, 1900, La Plata, Buenos Aires, Argentina.
authors.forename: Carlos
authors.initials: C
authors.lastname: Perfumo
authors.affiliation: CONICET, Av. Rivadavia 1917, C1033AAJ, CABA, Argentina; Instituto de Patobiología, CICVyA, INTA, Nicolas Repetto y de los Reseros s/n 1686, Hurlingham, Buenos Aires, Argentina.
authors.forename: Ariel
authors.initials: A
authors.lastname: Pereda
authors.affiliation: Poultry Diagnostic and Research Center, College of Veterinary Medicine, University of Georgia, 953 College Station Rd, Athens, GA, 30602, USA. Electronic address: dperez1@uga.edu.
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Pérez
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001118:Argentina / Q000453:epidemiology; D001996:Bronchopneumonia / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D003430:Cross-Sectional Studies; D062665:Epidemiological Monitoring; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000145:classification / Q000276:immunology / Q000302:isolation & purification; D057221:Influenza A Virus, H1N2 Subtype / Q000302:isolation & purification; D007251:Influenza, Human; D008168:Lung / Q000473:pathology / Q000821:virology; D009666:Nose / Q000821:virology; D009976:Orthomyxoviridae Infections / Q000453:epidemiology / Q000503:physiopathology / Q000662:veterinary* / Q000821:virology; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000821:virology; D017201:Virus Shedding
chemical_list: D000914:Antibodies, Viral
keywords: Argentina; Influenza; Pathology; Serology; Swine; Virology