title: Ultra-fast cell counters based on microtubular waveguides.
abstract: We present a radio-frequency impedance-based biosensor embedded inside a semiconductor microtube for the in-flow detection of single cells. An impedance-matched tank circuit and a tight wrapping of the electrodes around the sensing region, which creates a close, leakage current-free contact between cells and electrodes, yields a high signal-to-noise ratio. We experimentally show a twofold improved sensitivity of our three-dimensional electrode structure to conventional planar electrodes and support these findings by finite element simulations. Finally, we report on the differentiation of polystyrene beads, primary mouse T lymphocytes and Jurkat T lymphocytes using our device.
authors.affiliation: Institute of Nanostructure and Solid State Physics, University of Hamburg, Jungiusstraße 11c, Hamburg, Germany.|Center for Hybrid Nanostructures, University of Hamburg, Falkenried 88, Hamburg, Germany.
authors.forename: Cornelius S
authors.initials: CS
authors.lastname: Bausch
authors.affiliation: Institute of Nanostructure and Solid State Physics, University of Hamburg, Jungiusstraße 11c, Hamburg, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Heyn
authors.affiliation: Institute of Nanostructure and Solid State Physics, University of Hamburg, Jungiusstraße 11c, Hamburg, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Hansen
authors.affiliation: Calcium Signaling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, Martinistraße 52, Hamburg, Germany.
authors.forename: Insa M A
authors.initials: IM
authors.lastname: Wolf
authors.affiliation: Calcium Signaling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, Martinistraße 52, Hamburg, Germany.
authors.forename: Björn-Philipp
authors.initials: BP
authors.lastname: Diercks
authors.affiliation: Calcium Signaling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, Martinistraße 52, Hamburg, Germany.
authors.forename: Andreas H
authors.initials: AH
authors.lastname: Guse
authors.affiliation: Institute of Nanostructure and Solid State Physics, University of Hamburg, Jungiusstraße 11c, Hamburg, Germany.|Center for Hybrid Nanostructures, University of Hamburg, Falkenried 88, Hamburg, Germany.
authors.forename: Robert H
authors.initials: RH
authors.lastname: Blick
mesh_terms: D015374:Biosensing Techniques; D017097:Electric Impedance; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods; D006801:Humans; D008962:Models, Theoretical; D012666:Semiconductors; D013601:T-Lymphocytes / Q000378:metabolism