title: Direct competitive fluoroimmunoassays for detection of imidaclothiz in environmental and agricultural samples using quantum dots and europium as labels.
abstract: A direct quantum dots-based fluoroimmunoassay (QDFIA) and a time-resolved fluoroimmunoassay (TRFIA) for imidaclothiz (IMI) were developed by using the quantum dots (QDs)-labeled antibody and the europium (Eu3+)-labeled antibody, respectively. After optimization, the half-maximal inhibition concentration (IC50) and the limit of detection (LOD, IC10) are 20.41 and 0.52μgL-1for the QDFIA, while 6.91 and 0.018μgL-1for the TRFIA, respectively. The cross-reactivities (CRs) with the analogues are negligible except for imidacloprid with CRs of 29.0% for the QDFIA and 26.6% for the TRFIA. The spiked recoveries of IMI in paddy water, soil, pear, tomato, rice, apple, cabbage and cucumber are 72.7-117.6% with a standard deviation (RSD) of 2.4-13.5% for the QDFIA, and 81.3-117.7% with a RSD of 1.6-7.5% for TRFIA. The detection results of the analyses for the real paddy water and pear samples are markedly correlated with these of high-performance liquid chromatography (HPLC).
authors.affiliation: College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China.
authors.forename: Xiude
authors.initials: X
authors.lastname: Hua
authors.affiliation: College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ding
authors.affiliation: College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China.
authors.forename: Jiachuan
authors.initials: J
authors.lastname: Yang
authors.affiliation: College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Ma
authors.affiliation: College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Shi
authors.affiliation: College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China. Electronic address: wangmha@njau.edu.cn.
authors.forename: Minghua
authors.initials: M
authors.lastname: Wang
mesh_terms: D001937:Brassica / Q000737:chemistry; D004784:Environmental Monitoring / Q000379:methods*; D005063:Europium / Q000737:chemistry; D015200:Fluoroimmunoassay / Q000379:methods*; D018551:Solanum lycopersicum / Q000737:chemistry; D000073943:Neonicotinoids; D009574:Nitro Compounds; D012275:Oryza; D010573:Pesticide Residues / Q000032:analysis*; D045663:Quantum Dots / Q000737:chemistry; D013844:Thiazoles / Q000032:analysis*
chemical_list: D000073943:Neonicotinoids; D009574:Nitro Compounds; D010573:Pesticide Residues; D013844:Thiazoles; C082359:imidacloprid; D005063:Europium; C550087:imidaclothiz
keywords: Fluorescence immunoassay; Imidaclothiz; Monoclonal antibody; Quantum dots-based fluoroimmunoassay; Time-resolved fluoroimmunoassay