title: Mechanochemical Signaling Directs Cell-Shape Change.
abstract: For specialized cell function, as well as active cell behaviors such as division, migration, and tissue development, cells must undergo dynamic changes in shape. To complete these processes, cells integrate chemical and mechanical signals to direct force production. This mechanochemical integration allows for the rapid production and adaptation of leading-edge machinery in migrating cells, the invasion of one cell into another during cell-cell fusion, and the force-feedback loops that ensure robust cytokinesis. A quantitative understanding of cell mechanics coupled with protein dynamics has allowed us to account for furrow ingression during cytokinesis, a model cell-shape-change process. At the core of cell-shape changes is the ability of the cell's machinery to sense mechanical forces and tune the force-generating machinery as needed. Force-sensitive cytoskeletal proteins, including myosin II motors and actin cross-linkers such as α-actinin and filamin, accumulate in response to internally generated and externally imposed mechanical stresses, endowing the cell with the ability to discern and respond to mechanical cues. The physical theory behind how these proteins display mechanosensitive accumulation has allowed us to predict paralog-specific behaviors of different cross-linking proteins and identify a zone of optimal actin-binding affinity that allows for mechanical stress-induced protein accumulation. These molecular mechanisms coupled with the mechanical feedback systems ensure robust shape changes, but if they go awry, they are poised to promote disease states such as cancer cell metastasis and loss of tissue integrity.
authors.affiliation: Department of Cell Biology, Johns Hopkins University, Baltimore, Maryland.
authors.forename: Eric S
authors.initials: ES
authors.lastname: Schiffhauer
authors.affiliation: Department of Cell Biology, Johns Hopkins University, Baltimore, Maryland; Department of Pharmacology and Molecular Science, Johns Hopkins University, Baltimore, Maryland; Department of Medicine, School of Medicine, Johns Hopkins University, Baltimore, Maryland; Department of Chemical and Biomolecular Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, Maryland. Electronic address: dnr@jhmi.edu.
authors.forename: Douglas N
authors.initials: DN
authors.lastname: Robinson
mesh_terms: D000818:Animals; D048430:Cell Shape; D040542:Mechanotransduction, Cellular