title: Dynamics of 45S rDNA sites in the cell cycle: fragile sites and chromosomal stability in Lolium and Festuca.
abstract: Analyses carried out with fluorescence in situ hybridization (FISH) in C-metaphases of the Lolium-Festuca complex have shown the occurrence of spontaneous fragile sites (FSs) in 45S rDNA regions. FSs are expressed as gaps but they do not result in breaks or chromosomal fragments in these species. These gaps have high DNA condensation observed as thin chromatin fibers that connect the apparent segments of the fragile chromosome, allowing for genomic stability. Assessing the behavior of these regions in the cell cycle of Lolium and Festuca species may lead to a better understanding of the dynamics that preserve stability during cell division. Furthermore, it is interesting to track the dynamics of chromosomes bearing 45S rDNA sites in the cell cycle as well as to observe the expression of FSs with no effect of the mitotic block. We observed variation in both the number and size of 45S FISH signals from the S/G2 phases of interphase and from prophase to anaphase where gaps in 45S rDNA sites also were observed. The change in the degree of condensation of the 45S site begins in the S/G2 phase and appears to be related to the transcriptional demand. Taking into account that the number of 45S rDNA sites tends to be re-established when cells reach telophase, we suggest that the chromatin fiber goes back to the normal condensation level to the anaphase (after segregation), allowing for the approximation of chromosome segments and ensuring dynamics that favor the genomic stability of these species.
authors.affiliation: Universidade Federal de Lavras, Departamento de Biologia, Lavras, MG, Brasil.
authors.forename: L C
authors.initials: LC
authors.lastname: Rocha
authors.affiliation: Universidade Federal de Lavras, Departamento de Biologia, Lavras, MG, Brasil.
authors.forename: G A
authors.initials: GA
authors.lastname: Silva
authors.affiliation: Universidade Federal de Lavras, Departamento de Biologia, Lavras, MG, Brasil.
authors.forename: F O
authors.initials: FO
authors.lastname: Bustamante
authors.affiliation: Universidade Federal de Lavras, Departamento de Biologia, Lavras, MG, Brasil.
authors.forename: R A D
authors.initials: RA
authors.lastname: Silveira
authors.affiliation: Embrapa Gado de Leite, Juiz de Fora, MG, Brasil.|Embrapa Clima Temperado, Pelotas, RS, Brasil.
authors.forename: A
authors.initials: A
authors.lastname: Mittlemann
authors.affiliation: Universidade Federal de Lavras, Departamento de Biologia, Lavras, MG, Brasil vhtechio@gmail.com.
authors.forename: V H
authors.initials: VH
authors.lastname: Techio
mesh_terms: D002453:Cell Cycle / Q000235:genetics*; D043171:Chromosomal Instability; D043283:Chromosome Fragile Sites; D031751:Festuca / Q000235:genetics*; D008129:Lolium / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*
chemical_list: D012335:RNA, Ribosomal; C088179:RNA, ribosomal, 45S