title: Kribbella soli sp. nov., isolated from soil.
abstract: A novel actinobacterial strain, designated FMN22T, was isolated from soil and characterised using a polyphasic approach. Strain FMN22Tshowed high 16S rRNA gene sequence similarity to Kribbella karoonensis Q41T(99.3%), Kribbella shirazensis UTMC 693T(99.0%), Kribbella aluminosa HKI 0478T(98.9%) and Kribbella hippodromi S1.4T(98.6%). Phylogenetic analysis using the 16S rRNA and concatenated gene (gyrB, rpoB, relA, recA and atpD) sequences showed that strain FMN22Tis closely related to the type strains of K. karoonensis DSM 17344T, K. shirazensis UTMC 693T, K. aluminosa HKI 0478T, K. hippodromi S1.4T, Kribbella jejuensis HD9Tand Kribbella solani DSA1T. Based on concatenated gene genetic distances analysis, strain FMN22Tis distinct from all Kribbella type strains. DNA-DNA hybridization experiments with closely related type strains, were found to be 59.2 ± 2.4, 54.8 ± 2.1, 16.4 ± 2.3 and 38.6 ± 2.5%, relatedness to K. karoonensis DSM 17344T, K. shirazensis DSM 45490T, K. aluminosa DSM 18824Tand K. jejuensis DSM 17305T, respectively. The cell wall peptidoglycan contained LL-diaminopimelic acid, and whole cell sugars were glucose, mannose and ribose. The predominant menaquinone was MK-9(H4). The major phospholipids are diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and phosphatidylinositol. Major fatty acids are anteiso-C15:0and iso-C16:0. These chemotaxonomic traits are in good agreement with those known for representatives of the genus Kribbella. A combination of DNA-DNA hybridization results and phenotypic properties demonstrated that strain FMN22Tcan be clearly distinguished from all close phylogenetic relatives. Therefore, strain FMN22T(=DSM 27132T= KCTC 29219T) is considered to be the type strain of a novel species of the genus Kribbella, for which the name Kribbella soli is proposed.
authors.affiliation: Department of Nursing, School of Health, Bilecik Seyh Edebali University, 11210, Bilecik, Turkey.
authors.forename: Fadime
authors.initials: F
authors.lastname: Ozdemir-Kocak
authors.affiliation: Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, 55139, Samsun, Turkey.
authors.forename: Hayrettin
authors.initials: H
authors.lastname: Saygin
authors.affiliation: Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, 55139, Samsun, Turkey.
authors.forename: Salih
authors.initials: S
authors.lastname: Saricaoglu
authors.affiliation: Science Teaching Programme, Gazi Faculty of Education, Gazi University, 06500, Ankara, Turkey.
authors.forename: Demet
authors.initials: D
authors.lastname: Cetin
authors.affiliation: Department of Biology, Faculty of ScienceAnadolu University, 26470, Eskisehir, Turkey.
authors.forename: Kiymet
authors.initials: K
authors.lastname: Guven
authors.affiliation: Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstrassse 7B, 38124, Brunswick, Germany.
authors.forename: Cathrin
authors.initials: C
authors.lastname: Spröer
authors.affiliation: Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstrassse 7B, 38124, Brunswick, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
authors.affiliation: School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne, NE1 7RU, UK.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Klenk
authors.affiliation: Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, 55139, Samsun, Turkey.
authors.forename: Nevzat
authors.initials: N
authors.lastname: Sahin
authors.affiliation: Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, 55139, Samsun, Turkey. kamilis@omu.edu.tr.
authors.forename: Kamil
authors.initials: K
authors.lastname: Isik
mesh_terms: D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D002473:Cell Wall / Q000737:chemistry; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020043:Genes, Essential; D058885:Multilocus Sequence Typing; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid
keywords: Actinobacteria; Kribbella soli; Nocardioidaceae; Polyphasic taxonomy