title: DNMT3B modulates the expression of cancer-related genes and downregulates the expression of the gene VAV3 via methylation.
abstract: Altered promoter DNA methylation is one of the most important epigenetic abnormalities in human cancer. DNMT3B,de novomethyltransferase, is clearly related to abnormal methylation of tumour suppressor genes, DNA repair genes and its overexpression contributes to oncogenic processes and tumorigenesisin vivo. The purpose of this study was to assess the effect of the overexpression of DNMT3B in HaCaT cells on global gene expression and on the methylation of selected genes to the identification of genes that can be target of DNMT3B. We found that the overexpression of DNMT3B in HaCaT cells, modulate the expression of genes related to cancer, downregulated the expression of 151 genes with CpG islands and downregulated the expression of the VAV3 gene via methylation of its promoter. These results highlight the importance of DNMT3B in gene expression and human cancer.
authors.affiliation: Laboratorio de Biomedicina Molecular, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero Chilpancingo, Guerrero, México.
authors.forename: Irlanda
authors.initials: I
authors.lastname: Peralta-Arrieta
authors.affiliation: Laboratorio de Epigenética del Cáncer, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero Chilpancingo, Guerrero, México.
authors.forename: Daniel
authors.initials: D
authors.lastname: Hernández-Sotelo
authors.affiliation: Laboratorio de Investigación en Citopatología e Histoquímica, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero Chilpancingo, Guerrero, México.
authors.forename: Yaneth
authors.initials: Y
authors.lastname: Castro-Coronel
authors.affiliation: Laboratorio de Biomedicina Molecular, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero Chilpancingo, Guerrero, México.
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: Leyva-Vázquez
authors.affiliation: Laboratorio de Biomedicina Molecular, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero Chilpancingo, Guerrero, México.
authors.forename: Berenice
authors.initials: B
authors.lastname: Illades-Aguiar
keywords: CpG island; Methylation; VAV3; cancer; cancer-related genes; de novo methyltransferase; overexpression of DNMT3B