title: A novel monoterpenoid indole alkaloid with anticancer activity from Melodinus khasianus.
abstract: A novel monoterpenoid indole alkaloid with unprecedented 6/5/6/4/6 fused rings, khasuanine A (1), was isolated from the roots of Melodinus khasianus. The structure was determined by extensive analysis of its HR-MS, 1D-, and 2D-NMR spectra. Khasuanine A markedly inhibited the proliferation of PC3 cell with IC50value of 0.45μM. Further study showed that khasuanine A was able to induce the apoptosis of PC3 cells by activation of caspase 3 and p53, and by inhibition of Bcl-2.
authors.affiliation: School of Biological Science and Technology, University of Jinan, Jinan 250022, PR China; Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Jinan 250014, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Jinan 250014, PR China.
authors.forename: Jin-Hong
authors.initials: JH
authors.lastname: Feng
authors.affiliation: School of Biological Science and Technology, University of Jinan, Jinan 250022, PR China; Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Jinan 250014, PR China; State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University, Guilin 541004, PR China. Electronic address: fleiv@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Fang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D029062:Apocynaceae / Q000737:chemistry* / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D006801:Humans; D026121:Indole Alkaloids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D039821:Monoterpenes / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000037:antagonists & inhibitors / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D026121:Indole Alkaloids; D039821:Monoterpenes; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; C000621045:khasuanine A; D053148:Caspase 3
keywords: Anticancer; Apoptosis; Melodinus khasianus; Monoterpenoid indole alkaloid