title: Pulsed magnetic field enhances therapeutic efficiency of mesenchymal stem cells in chronic neuropathic pain model.
abstract: Cell-based or magnetic field therapies as alternative approaches to pain management have been tested in several experimental pain models. The aim of this study therefore was to investigate the actions of the cell-based therapy (adipose tissue derived mesenchymal stem cells; ADMSC) or pulsed magnetic field (PMF) therapy and magneto-cell therapy (combination of ADMSC and PMF) in chronic constriction nerve injury model (CCI). The actions of individual ADMSC (route dependent [systemic or local], time-dependent [a day or a week after surgery]), or PMF and their combination (magneto-cell) therapies on hyperalgesia and allodynia were investigated by using thermal plantar test and a dynamic plantar aesthesiometer, respectively. In addition, various cytokine levels (IL-1β, IL-6, and IL-10) of rat sciatic nerve after CCI were analyzed. Following the CCI, both latency and threshold significantly decreased. ADMSC or PMF significantly increased latencies and thresholds. The combination of ADMSC with PMF even more significantly increased latency and threshold when compared with ADMSC alone. However, ADMSC-induced decrease in pro-inflammatory or increase in anti-inflammatory cytokines levels were partially prevented by PMF treatments. Present findings may suggest that both cell-based and magnetic therapies can effectively attenuate chronic neuropathic pain symptoms. Combined magneto-cell therapy may also efficiently reverse neuropathic signs. Bioelectromagnetics. 38:255-264, 2017. © 2017 Wiley Periodicals, Inc.
authors.affiliation: Department of Biophysics, School of Medicine, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey.
authors.forename: Tufan
authors.initials: T
authors.lastname: Mert
authors.affiliation: Department of Pharmacology, School of Medicine, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey.
authors.forename: Akif Hakan
authors.initials: AH
authors.lastname: Kurt
authors.affiliation: Department of Brain and Nerve Surgery, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey.
authors.forename: İdiris
authors.initials: İ
authors.lastname: Altun
authors.affiliation: Department of Biochemistry, School of Medicine, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Celik
authors.affiliation: School of Medicine (Medical Student), Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey.
authors.forename: Furkan
authors.initials: F
authors.lastname: Baran
authors.affiliation: Department of Biophysics, School of Medicine, Cukurova University, Adana, Turkey.
authors.forename: Ismail
authors.initials: I
authors.lastname: Gunay
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D064987:Cell- and Tissue-Based Therapy; D002908:Chronic Disease; D003131:Combined Modality Therapy; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D055909:Magnetic Field Therapy; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009437:Neuralgia / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000293:injuries
chemical_list: D016207:Cytokines
keywords:  pulsed magnetic field; adipose tissue derived mesenchymal stem cells; allodynia; chronic constriction injury model; cytokine; hyperalgesia