title: The chemical profiling of loquat leaf extract by HPLC-DAD-ESI-MS and its effects on hyperlipidemia and hyperglycemia in rats induced by a high-fat and fructose diet.
abstract: In this study, the inhibitory effects of loquat leaf extract (LLE) on pancreatic α-amylase and α-glucosidase, and the preventative effects of LLE on hyperlipidemia and hyperglycemia in rats induced by a high fat and fructose diet have been evaluated. The LLE was chemically described using a high performance liquid chromatography-diode array detector coupled with a mass spectrometer (HPLC-DAD-MS/MS). 20 compounds including phenolic acids, flavonoids and triterpene acids were tentatively identified with authentic compounds or by referring to published articles and accessible databases (e.g. MassBank, METLIN). Enzyme activity measurements showed that the IC50values of the LLE on α-amylase and α-glucosidase were 11.34 ± 1.04 mg mL-1and 50.77 ± 1.04 μg mL-1, respectively. The calculated Michaelis-Menten constants indicated that the LLE is an effective inhibitor against α-glucosidase in a mixed-model competitive mode. The fluorescence data revealed that the LLE binds with α-amylase and α-glucosidase. The animal experiment results indicated that the LLE significantly decreased the levels of fasting blood glucose, and hepatic and serum triglycerides.
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China. zhli2091@sina.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China. zhli2091@sina.com.
authors.forename: Piaopiao
authors.initials: P
authors.lastname: Long
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China. zhli2091@sina.com.
authors.forename: Yue
authors.initials: Y
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China. zhli2091@sina.com.
authors.forename: Qilu
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Institute of Agro-Products Processing Science and Technology (IAPPST), Chinese Academy of Agricultural Science, Beijing, 100193, China.
authors.forename: Xingxun
authors.identifier: http://orcid.org/0000-0001-9101-3525
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China. zhli2091@sina.com.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China. zhli2091@sina.com.
authors.forename: Qingya
authors.initials: Q
authors.lastname: Lv
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China. zhli2091@sina.com.
authors.forename: Liang
authors.identifier: http://orcid.org/0000-0002-2279-2657
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D059305:Diet, High-Fat / Q000009:adverse effects*; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000737:chemistry; D031983:Eriobotrya / Q000737:chemistry*; D005632:Fructose / Q000009:adverse effects* / Q000378:metabolism; D006801:Humans; D006943:Hyperglycemia / Q000188:drug therapy* / Q000201:enzymology / Q000378:metabolism; D006949:Hyperlipidemias / Q000188:drug therapy* / Q000201:enzymology / Q000378:metabolism; D007700:Kinetics; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D053719:Tandem Mass Spectrometry; D000516:alpha-Amylases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D000520:alpha-Glucosidases / Q000737:chemistry / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D010936:Plant Extracts; D005632:Fructose; D000516:alpha-Amylases; D000520:alpha-Glucosidases