title: Effect of ephrin-B2 on the expressions of angiopoietin-1 and -2 after focal cerebral ischemia/reperfusion.
abstract: Ephrin-B2 has been shown to participate in angiogenesis, but the underlying mechanisms involved remain unclear. In this study, a rat model of focal cerebral ischemia was prepared by focal middle cerebral artery occlusion, followed by 24-hour reperfusion. Then, ephrin-B2 protein was administered intracerebroventricularly for 3 consecutive daysviaa micro-osmotic pump. Western blot assay and quantitative real-time reverse transcription PCR demonstrated the expression levels of angiopoietin-1 (Ang-1) mRNA and protein in the penumbra cortex of the ephrin-B2 treated group were decreased at day 4 after reperfusion, and increased at day 28, while the expression levels of angiopoietin-2 (Ang-2) were highly up-regulated at all time points tested. Double immunofluorescent staining indicated that Ang-1 and Ang-2 were both expressed in vascular endothelial cells positive for CD31. These findings indicate that ephrin-B2 influences the expressions of Ang-1 and Ang-2 during angiogenesis following transient focal cerebral ischemia.
authors.affiliation: Department of Neurology, Changsha Municipal Central Hospital, Changsha, Hunan Province, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xiao
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
authors.forename: Jia-Qi
authors.initials: JQ
authors.lastname: Wang
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Deng
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
authors.forename: Wen-Ping
authors.identifier: 0000-0002-5018-4886
authors.initials: WP
authors.lastname: Gu
keywords: angiogenesis; angiopoietin-1; angiopoietin-2; ephrin-B2; focal cerebral ischemia/reperfusion; nerve regeneration; neuroprotection