title: Myogenic potential of human alveolar mucosa derived cells.
abstract: Difficulties related to the obtainment of stem/progenitor cells from skeletal muscle tissue make the search for new sources of myogenic cells highly relevant. Alveolar mucosa might be considered as a perspective candidate due to availability and high proliferative capacity of its cells. Human alveolar mucosa cells (AMC) were obtained from gingival biopsy samples collected from 10 healthy donors and cultured up to 10 passages. AMC matched the generally accepted multipotent mesenchymal stromal cells criteria and possess population doubling time, caryotype and immunophenotype stability during long-term cultivation. The single myogenic induction of primary cell cultures resulted in differentiation of AMC into multinucleated myotubes. The myogenic differentiation was associated with expression of skeletal muscle markers: skeletal myosin, skeletal actin, myogenin and MyoD1. Efficiency of myogenic differentiation in AMC cultures was similar to that in skeletal muscle cells. Furthermore, some of differentiated myotubes exhibited contractions in vitro. Our data confirms the sufficiently high myogenic potential and proliferative capacity of AMC and their ability to maintain in vitro proliferation-competent myogenic precursor cells regardless of the passage number.
authors.affiliation: a FSBI "Central Clinical Hospital with Outpatient Health Center" , Moscow , Russia.|b Department of Regenerative Medicine at the Human Stem Cells Institute , Moscow , Russia.
authors.forename: Vadim L
authors.initials: VL
authors.lastname: Zorin
authors.affiliation: a FSBI "Central Clinical Hospital with Outpatient Health Center" , Moscow , Russia.
authors.forename: Andrey A
authors.initials: AA
authors.lastname: Pulin
authors.affiliation: a FSBI "Central Clinical Hospital with Outpatient Health Center" , Moscow , Russia.
authors.forename: Ilya I
authors.initials: II
authors.lastname: Eremin
authors.affiliation: a FSBI "Central Clinical Hospital with Outpatient Health Center" , Moscow , Russia.
authors.forename: Ivan N
authors.initials: IN
authors.lastname: Korsakov
authors.affiliation: b Department of Regenerative Medicine at the Human Stem Cells Institute , Moscow , Russia.
authors.forename: Alla I
authors.initials: AI
authors.lastname: Zorina
authors.affiliation: c Blokhin Russian Cancer Research Center , Moscow , Russia.
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Khromova
authors.affiliation: c Blokhin Russian Cancer Research Center , Moscow , Russia.
authors.forename: Olga I
authors.initials: OI
authors.lastname: Sokova
authors.affiliation: d FSBI "Central State Medical Academy" of Business Administration for the President of the Russian Federation , Moscow , Russia.
authors.forename: Konstantin V
authors.initials: KV
authors.lastname: Kotenko
authors.affiliation: c Blokhin Russian Cancer Research Center , Moscow , Russia.
authors.forename: Pavel B
authors.initials: PB
authors.lastname: Kopnin
mesh_terms: D050156:Adipogenesis; D000328:Adult; D002454:Cell Differentiation; D048430:Cell Shape; D020219:Chondrogenesis; D005260:Female; D005881:Gingiva / Q000166:cytology; D006801:Humans; D007621:Karyotyping; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology; D008875:Middle Aged; D009092:Mucous Membrane / Q000166:cytology*; D024510:Muscle Development; D032389:Myocytes, Smooth Muscle / Q000166:cytology; D058628:Myofibroblasts / Q000166:cytology / Q000378:metabolism; D010012:Osteogenesis; D011650:Pulmonary Alveoli / Q000166:cytology* / Q000378:metabolism
keywords: alveolar mucosa; gingiva; multipotent differentiation; multipotent mesenchymal stromal cells; muotubes; myoblasts; myogenic differentiation; regenerative medicine