title: Removal of pharmaceuticals from municipal wastewaters at laboratory scale by treatment with activated sludge and biostimulation.
abstract: Municipal wastewater containing 21 pharmaceutical compounds, as well as activated sludge obtained from the aeration tank of the same wastewater treatment plant were used in lab-scale biodegradation experiments. The concentrations of pharmaceutical compounds were determined by high-performance liquid chromatography coupled to Orbitrap high-resolution mass spectrometry and ranged from 13.2ng/L to 51.8μg/L. Activated sludge was characterized in the terms of phylogenetic and catabolic diversity of microbial community, as well as its morphology. Proteobacteria (24.0%) represented the most abundant phylum, followed by Bacteroidetes (19.8%) and Firmicutes (13.2%). Bioaugmentation of wastewater with activated sludge stimulated the biodegradation process for 14 compounds. The concentration of carbamazepine in non-amended and bioaugmented WW decreased during the first 17h up to 30% and 70%, respectively. Diclofenac and ibuprofen demonstrated comparatively slow removal. The stimulating effect of the added nutrients was observed for the degradation of almost all pharmaceuticals detected in WW. The most pronounced effect of nutrients was found for erythromycin. The results were compared with those obtained for the full-scale WW treatment process.
authors.affiliation: Institute of Microbiology & Biotechnology, University of Latvia, 1 Jelgavas Str., Riga LV-1004, Latvia. Electronic address: olga.muter@inbox.lv.
authors.forename: Olga
authors.initials: O
authors.lastname: Muter
authors.affiliation: Faculty of Chemistry, University of Latvia, 1 Jelgavas Str., Riga LV-1004, Latvia.
authors.forename: Ingus
authors.initials: I
authors.lastname: P Erkons
authors.affiliation: Faculty of Biology, University of Latvia, 1 Jelgavas Str., Riga LV-1004, Latvia.
authors.forename: Turs
authors.initials: T
authors.lastname: Selga
authors.affiliation: Institute of Microbiology & Biotechnology, University of Latvia, 1 Jelgavas Str., Riga LV-1004, Latvia.
authors.forename: Andrejs
authors.initials: A
authors.lastname: Berzins
authors.affiliation: Latvian Biomedical Research and Study Center, 1 Ratsupites Str., Riga LV-1067, Latvia.
authors.forename: Dita
authors.initials: D
authors.lastname: Gudra
authors.affiliation: Latvian Biomedical Research and Study Center, 1 Ratsupites Str., Riga LV-1067, Latvia.
authors.forename: Ilze
authors.initials: I
authors.lastname: Radovica-Spalvina
authors.affiliation: Latvian Biomedical Research and Study Center, 1 Ratsupites Str., Riga LV-1067, Latvia.
authors.forename: Davids
authors.initials: D
authors.lastname: Fridmanis
authors.affiliation: Faculty of Chemistry, University of Latvia, 1 Jelgavas Str., Riga LV-1004, Latvia.
authors.forename: Vadims
authors.initials: V
authors.lastname: Bartkevics
mesh_terms: D001419:Bacteria / Q000145:classification / Q000378:metabolism; D004364:Pharmaceutical Preparations / Q000302:isolation & purification*; D010802:Phylogeny; D012722:Sewage; D014865:Waste Disposal, Fluid; D062065:Wastewater; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*
chemical_list: D004364:Pharmaceutical Preparations; D012722:Sewage; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Antimicrobials; Biodegradation; Ion-Torrent Next-Generation Sequencing; Pharmaceuticals; Wastewater