title: Expression of the cytoplasmic nucleolin for post-transcriptional regulation of macrophage colony-stimulating factor mRNA in ovarian and breast cancer cells.
abstract: The formation of the mRNP complex is a critical component of translational regulation and mRNA decay. Both the 5' and 3'UTRs of CSF-1 mRNA are involved in post-transcriptional regulation. In CSF-1 mRNA, a small hairpin loop structure is predicted to form at the extreme 5' end (2-21nt) of the 5'UTR. Nucleolin binds the hairpin loop structure in the 5'UTR of CSF-1 mRNA and enhances translation, while removal of this hairpin loop nucleolin binding element dramatically represses translation. Thus in CSF-1 mRNA, the hairpin loop nucleolin binding element is critical for translational regulation. In addition, nucleolin interacts with the 3'UTR of CSF-1 mRNA and facilitates the miRISC formation which results in poly (A) tail shortening. The overexpression of nucleolin increases the association of CSF-1 mRNA containing short poly (A)n≤26, with polyribosomes. Nucleolin both forms an mRNP complex with the eIF4G and CSF-1 mRNA, and is co-localized with the eIF4G in the cytoplasm further supporting nucleolin's role in translational regulation. The distinct foci formation of nucleolin in the cytoplasm of ovarian and breast cancer cells implicates the translational promoting role of nucleolin in these cancers.
authors.affiliation: University of Arizona Cancer Center, Tucson, AZ 85724, USA. Electronic address: hhwoo@email.arizona.edu.
authors.forename: Ho-Hyung
authors.initials: HH
authors.lastname: Woo
authors.affiliation: University of Arizona Cancer Center, Tucson, AZ 85724, USA.
authors.forename: Sang C
authors.initials: SC
authors.lastname: Lee
authors.affiliation: University of Arizona Cancer Center, Tucson, AZ 85724, USA.
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Gibson
authors.affiliation: University of Arizona Cancer Center, Tucson, AZ 85724, USA.
authors.forename: Setsuko K
authors.initials: SK
authors.lastname: Chambers
mesh_terms: D020121:5' Untranslated Regions; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D039603:Eukaryotic Initiation Factor-4G / Q000235:genetics / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000096:biosynthesis* / Q000235:genetics; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D009690:Nucleic Acid Conformation; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism*; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000235:genetics; D011132:Polyribosomes / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D000097796:Nucleolin
chemical_list: D020121:5' Untranslated Regions; D039603:Eukaryotic Initiation Factor-4G; D016173:Macrophage Colony-Stimulating Factor; D009363:Neoplasm Proteins; D010750:Phosphoproteins; D012334:RNA, Neoplasm; D016601:RNA-Binding Proteins; D000097796:Nucleolin; C113920:EIF4G1 protein, human
keywords: Cytoplasmic nucleolin; Deadenylation; Hairpin loop structure; Macrophage colony stimulating factor (CSF-1) mRNA; Translation; Untranslated regions; eIF4G