title: Rapid identification and simultaneous analysis of multiple constituents from Rheum tanguticum Maxim. ex Balf. by UPLC/Q-TOF-MS.
abstract: Rhubarb contains biologically active compounds such as anthraquinones, anthrones, stilbenes and tannins. A rapid and efficient UPLC/Q-TOF-MS/MS method was developed and applied towards identifying the constituents of Rheum tanguticum Maxim. ex Balf. for the first time. Chemical constituents were separated and investigated by UPLC/Q-TOF-MS/MS in the negative ion mode. The ESI-MS2fragmentation pathways of four types of compounds were interpreted, providing a very useful guidance for the characterisation of different types of compounds. Based on the exact mass information, fragmentation characteristic and LC retention time of 7 reference standards, 30 constituents were tentatively identified from the methanol extract of R. tanguticum. Among them, seven compounds were described for the first time from R. tanguticum and two from the genus Rheum were described for the first time. The analytical tool used here is valuable for the rapid separation and identification of multiple and minor constituents in methanol extracts of R. tanguticum.
authors.affiliation: a Food and Drug College , Anhui Science and Technology University , Fengyang , China.|c Institute of Medicinal Plant Development , Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing , China.
authors.forename: Liang-Liang
authors.initials: LL
authors.lastname: Gao
authors.affiliation: b School of Life Science and Engineering , Lanzhou University of Technology , Lanzhou , China.
authors.forename: Tao
authors.initials: T
authors.lastname: Guo
authors.affiliation: c Institute of Medicinal Plant Development , Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing , China.
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Xu
authors.affiliation: c Institute of Medicinal Plant Development , Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing , China.
authors.forename: Jun-Shan
authors.initials: JS
authors.lastname: Yang
mesh_terms: D000880:Anthraquinones / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D000432:Methanol; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry; D012250:Rheum / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013267:Stilbenes / Q000032:analysis / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000880:Anthraquinones; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D013267:Stilbenes; D000432:Methanol
keywords: R. tanguticum; UPLC/Q-TOF-MS; fragmentation pathways; identification