title: Epigenetic map and genetic map basis of complex traits in cassava population.
abstract: Cassava (Manihot esculenta Crantz) is an important tropical starchy root crop that is adapted to drought but extremely cold sensitive. A cold-tolerant, high-quality, and robust supply of cassava is urgently needed. Here, we clarify genome-wide distribution and classification of CCGG hemi-methylation and full-methylation, and detected 77 much candidate QTLsepifor cold stress and 103 much candidate QTLsepifor storage root quality and yield in 186 cassava population, generated by crossing two non-inbred lines with female parent KU50 and male parent SC124 (KS population). We developed amplified-fragment single nucleotide polymorphism and methylation (AFSM) genetic map in this population. We also constructed the AFSM QTL map, identified 260 much candidate QTL genes for cold stress and 301 much candidate QTL genes for storage root quality and yield, based on the years greenhouse and field trials. This may accounted for a significant amount of the variation in the key traits controlling cold tolerance and the high quality and yield of cassava.
authors.affiliation: Huazhong Agricultural University, Wuhan, China.|The Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Zou
authors.affiliation: The Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Lu
authors.affiliation: The Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China.
authors.forename: Shengkui
authors.initials: S
authors.lastname: Zhang
authors.affiliation: The Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Chen
authors.affiliation: The Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China.
authors.forename: Xianglai
authors.initials: X
authors.lastname: Sun
authors.affiliation: The Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China.
authors.forename: Pingan
authors.initials: P
authors.lastname: Ma
authors.affiliation: The Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China.
authors.forename: Meizhen
authors.initials: M
authors.lastname: Hu
authors.affiliation: The Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Peng
authors.affiliation: The Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China.
authors.forename: Zilong
authors.initials: Z
authors.lastname: Ma
authors.affiliation: The Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.affiliation: The Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China.
authors.forename: Xincheng
authors.initials: X
authors.lastname: Zhou
authors.affiliation: The Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
authors.affiliation: The Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China.
authors.forename: Subin
authors.initials: S
authors.lastname: Feng
authors.affiliation: The Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China.
authors.forename: Kaixin
authors.initials: K
authors.lastname: Fang
authors.affiliation: The Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China.
authors.forename: Hairong
authors.initials: H
authors.lastname: Xie
authors.affiliation: Huazhong Agricultural University, Wuhan, China.
authors.forename: Zaiyun
authors.initials: Z
authors.lastname: Li
authors.affiliation: Huazhong Agricultural University, Wuhan, China.
authors.forename: Kede
authors.initials: K
authors.lastname: Liu
authors.affiliation: The Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China.
authors.forename: Qiongyao
authors.initials: Q
authors.lastname: Qin
authors.affiliation: The Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China.
authors.forename: Jinli
authors.initials: J
authors.lastname: Pei
authors.affiliation: The Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China.
authors.forename: Shujuan
authors.initials: S
authors.lastname: Wang
authors.affiliation: The Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Pan
authors.affiliation: The Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Hu
authors.affiliation: The Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China.
authors.forename: Binxiao
authors.initials: B
authors.lastname: Feng
authors.affiliation: Guangxi Academy of Agricultural Sciences, Guilin, China.
authors.forename: Dayong
authors.initials: D
authors.lastname: Fan
authors.affiliation: Guangxi Academy of Agricultural Sciences, Guilin, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Guangxi Academy of Agricultural Sciences, Guilin, China.
authors.forename: Chunling
authors.initials: C
authors.lastname: Wu
authors.affiliation: Guangxi Academy of Agricultural Sciences, Guilin, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Su
authors.affiliation: Huazhong Agricultural University, Wuhan, China.|The Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Xia
authors.affiliation: The Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China.
authors.forename: Kaimian
authors.initials: K
authors.lastname: Li
authors.affiliation: The Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China.
authors.forename: Wenquan
authors.initials: W
authors.lastname: Wang
mesh_terms: D058639:Cold-Shock Response; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D008040:Genetic Linkage; D018745:Genome, Plant; D002365:Manihot / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D019655:Quantitative Trait, Heritable