title: Co-Immobilization of Enzymes and Magnetic Nanoparticles by Metal-Nucleotide Hydrogelnanofibers for Improving Stability and Recycling.
abstract: In this paper we report a facile method for preparing co-immobilized enzyme and magnetic nanoparticles (MNPs) using metal coordinated hydrogel nanofibers.Candida rugosalipase (CRL) was selected as guest protein. For good aqueous dispersity, low price and other unique properties, citric acid-modified magnetic iron oxide nanoparticles (CA-Fe₃O₄ NPs) have been widely used for immobilizing enzymes. As a result, the relative activity of CA-Fe₃O₄@Zn/AMP nanofiber-immobilized CRL increased by 8-fold at pH 10.0 and nearly 1-fold in a 50 °C water bath after 30 min, compared to free CRL. Moreover, the immobilized CRL had excellent long-term storage stability (nearly 80% releative activity after storage for 13 days). This work indicated that metal-nucleotide nanofibers could efficiently co-immobilize enzymes and MNPs simultaneously, and improve the stability of biocatalysts.
authors.affiliation: Department of Environment Protection and Detection, Beijing Industrial Technician College, Beijing 100023, China. bitclichunfang@163.com.
authors.forename: Chunfang
authors.initials: C
authors.lastname: Li
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China. 18810976705@163.com.
authors.forename: Shuhui
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Beijing Centre for Physical and Chemical Analysis, Beijing 100089, China. Zhaoxinying_123@sina.com.
authors.forename: Xinying
authors.initials: X
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China. lianghao@mail.buct.edu.cn.
authors.forename: Hao
authors.initials: H
authors.lastname: Liang
mesh_terms: D055162:Biocatalysis; D002175:Candida / Q000201:enzymology*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D020100:Hydrogels; D058185:Magnetite Nanoparticles; D008049:Lipase
keywords: Candida rugose lipase (CRL); immobilized enzyme; magnetic iron oxide nanoparticles; metal; nucleotide