title: Distribution of Tomato spotted wilt virus in dahlia plants.
abstract: Tomato spotted wilt virus (TSWV) causes significant losses in the production of the ornamental plant Dahlia variabilis in Japan. The purpose of this study was to examine the distribution of TSWV in dahlia plants and identify plant parts that can be used in the selection of TSWV-free plants. The distribution of TSWV was investigated using reverse transcriptional polymerase chain reaction (RT-PCR) and tissue blot immunoassay. The detection rate of TSWV in latent infected compound leaves was the highest in the petiole, and it decreased from the veins and rachis to the lamina. The tissue blot immunoassays of the leaflets showed an uneven distribution of TSWV, especially along the edge of the leaf blade. In stems, the detection rate of TSWV was high partway up the stem compared to that in the upper and the lower parts of the stem during the vegetative growth stage. A highly uneven distribution was observed in the bulb. Our results indicated that middle parts of the stem as well as the petioles, rachis, and veins of compound leaves are suitable for detection of TSWV in dahlias. This study is the first to report uneven distribution of TSWV in dahlia plants.
  
  
  CONCLUSIONS
  In this study, the distribution of Tomato spotted wilt virus (TSWV) in various parts of dahlia plants was investigated for the first time. The distribution of TSWV was uneven in compound leaves, leaflets, stems, and bulbs. The middle parts of the stem or the petiole and leaf veins should be sampled to detect TSWV when selecting healthy plants.
authors.affiliation: Nara Prefecture Agricultural Research and Development Center, Sakurai, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Asano
authors.affiliation: Nara Prefecture Agricultural Research and Development Center, Sakurai, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Hirayama
authors.affiliation: Institute of Vegetable and Floriculture Science, NARO, Tsukuba, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Matsushita
mesh_terms: D031944:Dahlia / Q000821:virology*; D007564:Japan; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D018517:Plant Roots / Q000821:virology; D018547:Plant Stems / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017865:Tospovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*
keywords: 
  Dahlia variabilis
  ; 
  Tomato spotted wilt virus
  ; distribution; reverse transcriptional polymerase chain reaction; tissue blot immunoassay