title: Ca2+dynamics in zebrafish morphogenesis.
abstract: Intracellular calcium ion (Ca2+) signaling is heavily involved in development, as illustrated by the use of a number of Ca2+indicators. However, continuous Ca2+patterns during morphogenesis have not yet been studied using fluorescence resonance energy transfer to track the Ca2+sensor. In the present study, we monitored Ca2+levels during zebrafish morphogenesis and differentiation with yellow cameleon, YC2.12. Our results show not only clear changes in Ca2+levels but also continuous Ca2+patterns at 24 hpf and later periods for the first time. Serial Ca2+dynamics during early pharyngula period (Prim-5-20; 24-33 hpf) was successfully observed with cameleon, which have not reported anywhere yet. In fact, high Ca2+level occurred concurrently with hindbrain development in segmentation and pharyngula periods. Ca2+patterns in the late gastrula through segmentation periods which were obtained with cameleon, were similar to those obtained previously with other Ca2+sensor. Our results suggested that the use of various Ca2+sensors may lead to novel findings in studies of Ca2+dynamics. We hope that these results will prove valuable for further research in Ca2+signaling.
authors.affiliation: Marine Bioresource Exploration Research Group, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Tsuruwaka
authors.affiliation: Marine Bioresource Exploration Research Group, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan.|Department of Animal Science, University of California, Davis, CA, United States.|Cellevolt, Yokohama, Japan.
authors.forename: Eriko
authors.initials: E
authors.lastname: Shimada
authors.affiliation: Marine Bioresource Exploration Research Group, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Tsutsui
authors.affiliation: Marine Bioresource Exploration Research Group, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan.
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Ogawa
keywords: Intracellular calcium; Yellow cameleon; Zebrafish