title: Clinical significance of the long noncoding RNA RP11-19P22.6-001 in gastric cancer.
abstract: BACKGROUND
  Long non-coding RNAs (lncRNAs) play roles in carcinogenesis; however, the significance of most lncRNAs in gastric cancer is unclear.
  
  
  OBJECTIVE
  To explore the diagnostic value of the long noncoding RNA RP11-19P22.6-001 in gastric cancer.
  
  
  METHODS
  RP11-19P22.6-001 levels in gastric cancer tissues and paired non-tumor tissues were analyzed by quantitative reverse transcription-polymerase chain reaction. Since RP11-19P22.6-001 acts in cis to regulate nitric oxide synthase 2 (NOS2), we also analyzed NOS2 expression and its correlation with gastric cancer. Finally, to analyze the potential diagnostic values of RP11-19P22.6-001, a receiver operating characteristic (ROC) curve was constructed.
  
  
  RESULTS
  RP11-19P22.6-001 expression was significantly downregulated in 70.91% (78/110) of gastric cancer tissues compared to that of adjacent normal tissues. However, NOS2 was upregulated in 75.45% (83/110) of gastric cancer tissues. RP11-19P22.6-001 expression levels were associated with invasion, lymph node metastasis, and TNM stage. The areas under the ROC curves (AUC) were 0.662 and 0.671 for RP11-19P22.6-001 and NOS2, respectively. More importantly, the combined use of these parameters increased the AUC to 0.704.
  
  
  CONCLUSIONS
  RP11-19P22.6-001 and NOS2 may be new biomarkers for the diagnosis and prognosis of gastric cancer. The combined use of lncRNAs and their target genes may be a promising method to increase the diagnostic value of lncRNAs in cancer.
authors.affiliation: Ningbo Yinzhou People's Hospital and the Affiliated Yinzhou Hospital, Ningbo University School of Medicine, Ningbo 315040, Zhejiang, China.
authors.forename: Weiliang
authors.initials: W
authors.lastname: Sun
authors.affiliation: Department of Biochemistry and Molecular Biology, Zhejiang Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo 315211, Zhejiang, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Mo
authors.affiliation: Department of Biochemistry and Molecular Biology, Zhejiang Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo 315211, Zhejiang, China.
authors.forename: Tianwen
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, Zhejiang Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo 315211, Zhejiang, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Biochemistry and Molecular Biology, Zhejiang Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo 315211, Zhejiang, China.
authors.forename: Junming
authors.initials: J
authors.lastname: Guo
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D052247:Nitric Oxide Synthase Type II / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000235:genetics*; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D062085:RNA, Long Noncoding; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II
keywords: Gastric cancer; RP11-19P22.6-001; biomarker; long non-coding RNA; nitric oxide synthase 2