title: Coincident postsynaptic activity gates presynaptic dopamine release to induce plasticity inDrosophilamushroom bodies.
abstract: Simultaneous stimulation of the antennal lobes (ALs) and the ascending fibers of the ventral nerve cord (AFV), two sensory inputs to the mushroom bodies (MBs), induces long-term enhancement (LTE) of subsequent AL-evoked MB responses. LTE induction requires activation of at least three signaling pathways to the MBs, mediated by nicotinic acetylcholine receptors (nAChRs), NMDA receptors (NRs), and D1 dopamine receptors (D1Rs). Here, we demonstrate that inputs from the AL are transmitted to the MBs through nAChRs, and inputs from the AFV are transmitted by NRs. Dopamine signaling occurs downstream of both nAChR and NR activation, and requires simultaneous stimulation of both pathways. Dopamine release requires the activity of the rutabaga adenylyl cyclase in postsynaptic MB neurons, and release is restricted to MB neurons that receive coincident stimulation. Our results indicate that postsynaptic activity can gate presynaptic dopamine release to regulate plasticity.
authors.affiliation: Learning and Memory Project, Tokyo Metropolitan Institute of Medical Science, Setagaya, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Ueno
authors.affiliation: Learning and Memory Project, Tokyo Metropolitan Institute of Medical Science, Setagaya, Japan.
authors.forename: Ema
authors.initials: E
authors.lastname: Suzuki
authors.affiliation: Learning and Memory Project, Tokyo Metropolitan Institute of Medical Science, Setagaya, Japan.
authors.forename: Shintaro
authors.initials: S
authors.lastname: Naganos
authors.affiliation: Learning and Memory Project, Tokyo Metropolitan Institute of Medical Science, Setagaya, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Ofusa
authors.affiliation: Learning and Memory Project, Tokyo Metropolitan Institute of Medical Science, Setagaya, Japan.
authors.forename: Junjiro
authors.initials: J
authors.lastname: Horiuchi
authors.affiliation: Learning and Memory Project, Tokyo Metropolitan Institute of Medical Science, Setagaya, Japan.
authors.forename: Minoru
authors.identifier: 0000-0001-9731-4214
authors.initials: M
authors.lastname: Saitoe
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D057506:Arthropod Antennae / Q000502:physiology; D004298:Dopamine / Q000378:metabolism*; D004330:Drosophila / Q000502:physiology*; D029721:Drosophila Proteins / Q000378:metabolism*; D024521:Mushroom Bodies / Q000502:physiology*; D009473:Neuronal Plasticity; D013569:Synapses / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D000262:Adenylyl Cyclases; C479926:Rut protein, Drosophila; D004298:Dopamine
keywords: D. melanogaster; dopamine; learning and memory; neuroscience; synaptic plasticity; synaptic transmission