title: Chromatographic Methods to Evaluate Nutritional Quality in Oat.
abstract: Oats (A. sativa L.) have an important and positive role in human diet and health. The health benefits of oats are attributed to its multifunctional characteristic and nutritional profile, being an important source of soluble dietary fiber, well-balanced proteins, unsaturated fatty acids, vitamins, essential minerals, and a good source of natural antioxidants. These antioxidants include the avenanthramides (Avns) and avenalumic acids, which are unique to oats among cereals. High-performance liquid chromatography allows a simultaneous quantification of free amino acids and biogenic amines in oat samples as their OPA/FMOC-CL (o-phthalaldehyde/9-fluorenylmethoxycarbonyl chloride) derivatives. In addition, an ultra-performance liquid chromatography/mass spectrometry method was developed to quantify and characterize avenanthramides contained in oat samples.
authors.affiliation: Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University, Ithaca, NY, USA. gm457@cornell.edu.
authors.forename: Gracia
authors.initials: G
authors.lastname: Montilla-Bascón
authors.affiliation: Proteomics and Metabolomics Facility, Colorado State University, Fort Collins, CO, USA.
authors.forename: Corey D
authors.initials: CD
authors.lastname: Broeckling
authors.affiliation: Plant Genomics Consultant, Ithaca, NY, USA.
authors.forename: Owen A
authors.initials: OA
authors.lastname: Hoekenga
authors.affiliation: Institute for Sustainable Agriculture, CSIC, Córdoba, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Prats
authors.affiliation: Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University, 417 Bradfield Hall, Ithaca, NY, 14853, Ireland.
authors.forename: Mark
authors.initials: M
authors.lastname: Sorrells
authors.affiliation: Agriculture and Food Science, University College Dublin, Dublin, Ireland.
authors.forename: Julio
authors.initials: J
authors.lastname: Isidro-Sánchez
mesh_terms: D000596:Amino Acids / Q000032:analysis; D018554:Avena / Q000737:chemistry*; D001679:Biogenic Amines / Q000032:analysis; D002845:Chromatography / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D013058:Mass Spectrometry; D009753:Nutritive Value; D010936:Plant Extracts / Q000737:chemistry; D062367:ortho-Aminobenzoates / Q000032:analysis
chemical_list: D000596:Amino Acids; D001679:Biogenic Amines; D010936:Plant Extracts; D062367:ortho-Aminobenzoates; C514463:avenanthramide-2C
keywords: Amino acids; Avenanthramides; Biogenic amines; HPLC; Oats; UPLC-MS