title: Measurement of Cardiac Angiotensin II by Immunoassays, HPLC-Chip/Mass Spectrometry, and Functional Assays.
abstract: The molecular mechanisms related to the effect of angiotensin II, its level on cardiac tissues, as well as its overexpression represent an important aspect of cardiovascular pharmacology and pathology. Severe alterations of cardiac functions are induced by hypertension including activation of circulating and local cardiac renin angiotensin systems. In this chapter, we are providing the methods and materials necessary for further investigation of this important topic.
authors.affiliation: School of Medicine, Medical Sciences Campus UPR, 365067, San Juan, PR, 00936, USA. walmor.de-mello@upr.edu.
authors.forename: Walmor C
authors.initials: WC
authors.lastname: De Mello
authors.affiliation: School of Medicine, Medical Sciences Campus UPR, 365067, San Juan, PR, 00936, USA.
authors.forename: Yamil
authors.initials: Y
authors.lastname: Gerena
mesh_terms: D000804:Angiotensin II / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D006973:Hypertension / Q000378:metabolism; D007118:Immunoassay / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D044140:Receptor, Angiotensin, Type 1 / Q000378:metabolism
chemical_list: D044140:Receptor, Angiotensin, Type 1; D000804:Angiotensin II
keywords: AT1 receptor; Angiotensin II; Cardiomyocytes; ELISA; Flow cytometry; HPLC-MS; Heart; Intracellular; Overexpression; Visualization