title: Determination of T-2 and HT-2 Toxins in Oats and Oat-Based Breakfast Cereals by Liquid-Chromatography Tandem Mass Spectrometry.
abstract: This protocol specifies an accurate and sensitive method for the determination of T-2 and HT-2 toxins content in oats and oat-based foods using liquid chromatography with tandem mass spectrometry detection. T-2 and HT-2 toxins are extracted from the test material with a mixture of acetonitrile and water. The filtered extract is dried, reconstituted with a mixture of methanol and water, then purified on a polymeric solid-phase extraction cartridge. Toxins are finally eluted from the column with methanol and quantified by reversed phase high performance liquid chromatography with tandem mass spectrometry detection.
authors.affiliation: Institute of Sciences of Food Production (ISPA), National Research Council of Italy (CNR), Via G. Amendola, 122/O, 70126, Bari, Italy. veronica.lattanzio@ispa.cnr.it.
authors.forename: Veronica M T
authors.initials: VM
authors.lastname: Lattanzio
authors.affiliation: Institute of Sciences of Food Production (ISPA), National Research Council of Italy (CNR), Via G. Amendola, 122/O, 70126, Bari, Italy.
authors.forename: Michelangelo
authors.initials: M
authors.lastname: Pascale
mesh_terms: D018554:Avena / Q000382:microbiology*; D002853:Chromatography, Liquid / Q000379:methods; D002523:Edible Grain / Q000382:microbiology*; D005506:Food Contamination; D005516:Food Microbiology; D010936:Plant Extracts / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods; D013605:T-2 Toxin / Q000031:analogs & derivatives* / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D010936:Plant Extracts; D013605:T-2 Toxin; C012351:HT-2 toxin
keywords: Fusarium toxins; HT-2 toxin; Liquid chromatography; Mass spectrometry; Oats; Solid phase extraction; T-2 toxin