title: NADPH Oxidases and Measurement of Reactive Oxygen Species.
abstract: The NADPH oxidase (Nox) family of enzymes is expressed in many tissues that are involved in hypertension, including blood vessels, kidney, and brain. In these tissues, the products of NADPH oxidase activity, superoxide and ultimately hydrogen peroxide, act as intracellular and extracellular messengers during compartmentalized cellular signaling. The correct measurement of Nox activity and its products is crucial to enable studies of how these signaling pathways affect the molecular mechanisms underlying hypertension. Here, we describe methods for detection and measurement of hydrogen peroxide and superoxide derived from NADPH oxidases in biological samples such as cells and tissues.
authors.affiliation: Division of Cardiology, Department of Medicine, Emory University, 308 WMB, 101 Woodruff Circle, Atlanta, GA, 30322, USA.
authors.forename: Angelica
authors.initials: A
authors.lastname: Amanso
authors.affiliation: Division of Cardiology, Department of Medicine, Emory University, 308 WMB, 101 Woodruff Circle, Atlanta, GA, 30322, USA.
authors.forename: Alicia N
authors.initials: AN
authors.lastname: Lyle
authors.affiliation: Division of Cardiology, Department of Medicine, Emory University, 308 WMB, 101 Woodruff Circle, Atlanta, GA, 30322, USA. kgriend@emory.edu.
authors.forename: Kathy K
authors.initials: KK
authors.lastname: Griendling
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006861:Hydrogen Peroxide / Q000378:metabolism; D006973:Hypertension / Q000378:metabolism; D051379:Mice; D019255:NADPH Oxidases / Q000378:metabolism*; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D013481:Superoxides / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D013481:Superoxides; D006861:Hydrogen Peroxide; D019255:NADPH Oxidases
keywords: Hydrogen peroxide; NADPH oxidases; Reactive oxygen species measurement