title: Cell-Selective Bioorthogonal Metabolic Labeling of RNA.
abstract: Stringent chemical methods to profile RNA expression within discrete cellular populations remains a key challenge in biology. To address this issue, we developed a chemical-genetic strategy for metabolic labeling of RNA. Cell-specific labeling of RNA can be profiled and imaged using bioorthogonal chemistry. We anticipate that this platform will provide the community with a much-needed chemical toolset for cell-type specific profiling of cell-specific transcriptomes derived from complex biological systems.
authors.affiliation: Department of Pharmaceutical Sciences, ‡Department of Neurobiology, §Department of Developmental & Cellular Biology and ∥Department of Chemistry, University of California, Irvine , Irvine, California 92697, United States.
authors.forename: Kim
authors.initials: K
authors.lastname: Nguyen
authors.affiliation: Department of Pharmaceutical Sciences, ‡Department of Neurobiology, §Department of Developmental & Cellular Biology and ∥Department of Chemistry, University of California, Irvine , Irvine, California 92697, United States.
authors.forename: Michael
authors.initials: M
authors.lastname: Fazio
authors.affiliation: Department of Pharmaceutical Sciences, ‡Department of Neurobiology, §Department of Developmental & Cellular Biology and ∥Department of Chemistry, University of California, Irvine , Irvine, California 92697, United States.
authors.forename: Miles
authors.initials: M
authors.lastname: Kubota
authors.affiliation: Department of Pharmaceutical Sciences, ‡Department of Neurobiology, §Department of Developmental & Cellular Biology and ∥Department of Chemistry, University of California, Irvine , Irvine, California 92697, United States.
authors.forename: Sarah
authors.initials: S
authors.lastname: Nainar
authors.affiliation: Department of Pharmaceutical Sciences, ‡Department of Neurobiology, §Department of Developmental & Cellular Biology and ∥Department of Chemistry, University of California, Irvine , Irvine, California 92697, United States.
authors.forename: Chao
authors.initials: C
authors.lastname: Feng
authors.affiliation: Department of Pharmaceutical Sciences, ‡Department of Neurobiology, §Department of Developmental & Cellular Biology and ∥Department of Chemistry, University of California, Irvine , Irvine, California 92697, United States.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Pharmaceutical Sciences, ‡Department of Neurobiology, §Department of Developmental & Cellular Biology and ∥Department of Chemistry, University of California, Irvine , Irvine, California 92697, United States.
authors.forename: Scott X
authors.initials: SX
authors.lastname: Atwood
authors.affiliation: Department of Pharmaceutical Sciences, ‡Department of Neurobiology, §Department of Developmental & Cellular Biology and ∥Department of Chemistry, University of California, Irvine , Irvine, California 92697, United States.
authors.forename: Timothy W
authors.initials: TW
authors.lastname: Bredy
authors.affiliation: Department of Pharmaceutical Sciences, ‡Department of Neurobiology, §Department of Developmental & Cellular Biology and ∥Department of Chemistry, University of California, Irvine , Irvine, California 92697, United States.
authors.forename: Robert C
authors.identifier: 0000-0002-3511-8098
authors.initials: RC
authors.lastname: Spitale
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D012313:RNA / Q000737:chemistry / Q000378:metabolism*
chemical_list: D012313:RNA