title: Salivary Diagnostics-Point-of-Care diagnostics of MMP-8 in dentistry and medicine.
abstract: Human saliva is an easily accessible biological fluid and contains a variety of disease-related biomarkers, which makes it a potential diagnostic medium. The clinical use of salivary/oral fluid biomarkers to identify oral and systemic conditions requires the development of non-invasive screening and diagnostic technologies, and is among the main goals of oral fluid researchers. The analysis of the disease-specific oral and systemic biomarkers in saliva and oral fluids (i.e., mouth-rinse, gingival crevicular fluid (GCF) and peri-implantitis sulcular fluid (PISF)) is demanding. Several factors influence their expression and release; these factors include the intracellular location, the molecular size and the flow characteristics of the biological fluid. The type of saliva/oral fluid utilized for the diagnostics affects the analysis. High sensitivity together with sophisticated methods and techniques are essential to get a useful outcome. We describe here a recently developed mouth-rinse that is practical, convenient and inexpensive, as well as PISF chair-side/point of care (PoC) lateral-flow active matrix metalloproteinase (aMMP-8) immunoassays to detect, predict and monitor the course and treatment of periodontitis and peri-implantitis.
authors.affiliation: Division of Periodontology, Department of Dental Medicine, Karolinska Institutet, 17177 Stockholm, Sweden. Nilminie.Rathnayake@ki.se.
authors.forename: Nilminie
authors.initials: N
authors.lastname: Rathnayake
authors.affiliation: Division of Periodontology, Dentognostics GmbH, 07745 Jena, Germany. gieselmann4@aol.com.
authors.forename: Dirk-Rolf
authors.initials: DR
authors.lastname: Gieselmann
authors.affiliation: Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki University Central Hospital, 00014 Helsinki, Finland. anna.m.heikkinen@helsinki.fi.
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Heikkinen
authors.affiliation: Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki University Central Hospital, 00014 Helsinki, Finland. taina.tervahartiala@helsinki.fi.
authors.forename: Taina
authors.initials: T
authors.lastname: Tervahartiala
authors.affiliation: Division of Periodontology, Department of Dental Medicine, Karolinska Institutet, 17177 Stockholm, Sweden. timo.sorsa@helsinki.fi.|Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki University Central Hospital, 00014 Helsinki, Finland. timo.sorsa@helsinki.fi.
authors.forename: Timo
authors.initials: T
authors.lastname: Sorsa
keywords: diagnostic test; peri-implantitis; periodontitis; point-of-care; systemic diseases