title: Reaction-Based Off-On Near-infrared Fluorescent Probe for Imaging Alkaline Phosphatase Activity in Living Cells and Mice.
abstract: Alkaline phosphatases are a group of enzymes that play important roles in regulating diverse cellular functions and disease pathogenesis. Hence, developing fluorescent probes for in vivo detection of alkaline phosphatase activity is highly desirable for studying the dynamic phosphorylation in living organisms. Here, we developed the very first reaction-based near-infrared (NIR) probe (DHXP) for sensitive detection of alkaline phosphatase activity both in vitro and in vivo. Our studies demonstrated that the probe displayed an up to 66-fold fluorescence increment upon incubation with alkaline phosphatases, and the detection limit of our probe was determined to be 0.07 U/L, which is lower than that of most of alkaline phosphatase probes reported in literature. Furthermore, we demonstrated that the probe can be applied to detecting alkaline phosphatase activity in cells and mice. In addition, our probe possesses excellent biocompatibility and rapid cell-internalization ability. In light of these prominent properties, we envision that DHXP will add useful tools for investigating alkaline phosphatase activity in biomedical research.
authors.affiliation: Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute of City University of Hong Kong , Shenzhen 518057, P. R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University , Xuzhou 221002, P. R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.forename: Ka Ho
authors.initials: KH
authors.lastname: Man
authors.affiliation: Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute of City University of Hong Kong , Shenzhen 518057, P. R. China.
authors.forename: Raoul
authors.initials: R
authors.lastname: Peltier
authors.affiliation: Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute of City University of Hong Kong , Shenzhen 518057, P. R. China.
authors.forename: Ganchao
authors.initials: G
authors.lastname: Chen
authors.affiliation: Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute of City University of Hong Kong , Shenzhen 518057, P. R. China.
authors.forename: Huatang
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute of City University of Hong Kong , Shenzhen 518057, P. R. China.
authors.forename: Liyi
authors.initials: L
authors.lastname: Zhou
authors.forename: Feng
authors.identifier: http://orcid.org/0000-0001-9471-4386
authors.initials: F
authors.lastname: Wang
authors.forename: Derek
authors.initials: D
authors.lastname: Ho
authors.affiliation: Department of Chemistry, National University of Singapore , Singapore 117543.
authors.forename: Shao Q
authors.initials: SQ
authors.lastname: Yao
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Multi-disciplinary Research Division, Institute of High Energy Physics, Chinese Academy of Sciences (CAS) , Beijing 100049, P. R. China.
authors.forename: Yi
authors.identifier: http://orcid.org/0000-0002-0952-0657
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute of City University of Hong Kong , Shenzhen 518057, P. R. China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Sun
mesh_terms: D000469:Alkaline Phosphatase; D000818:Animals; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D057230:Limit of Detection; D051379:Mice
chemical_list: D005456:Fluorescent Dyes; D000469:Alkaline Phosphatase
keywords: alkaline phosphatase; bioimaging; fluorescent probe; mice; near-infrared