title: Anomalous Separation of Small Y-Chromosomal DNA Fragments on Microchip Electrophoresis.
abstract: We investigated an anomalous DNA separation where two DNA fragments from the human Y-chromosome sY638 (64 bp) and sY592 (65 bp), with only one base pair difference, were separated. This result is abnormal since in a previous study, we found that 5 bp was the minimum difference between two DNA fragments that the microchip electrophoresis system can separate. The formation of a mini-loop in the structure of the DNA fragment of sY638 (64 bp) was strongly expected to be the reason. To investigate this, we synthesized three modified DNA fragments for sY638 (64 bp), and the modifications were in two expected locations for possible mini-loop formation. Later, the separation between sY592 (65 bp) and the three modified fragments of sY638 (64 bp) was not possible. Thus, we conclude that the formation of a mini-loop in the structure of the DNA is the reason behind this anomalous separation.
authors.affiliation: Department of Pharmaceutical Information Science, Institute of Health Biosciences, The University of Tokushima, 1-78-1 Sho-machi, 770-8505 Tokushima, Japan. jabasini1@gmail.com.|Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, 464-8603 Nagoya, Japan. jabasini1@gmail.com.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Jabasini
authors.affiliation: Department of Human Genetics Public Health, School of Medicine, The University of Tokushima, 3-18-15 Kuramoto-cho, 770-8503 Tokushima, Japan. ashraf_ewis@yahoo.com.
authors.forename: Ashraf
authors.initials: A
authors.lastname: Ewis
authors.affiliation: Department of Pharmaceutical Information Science, Institute of Health Biosciences, The University of Tokushima, 1-78-1 Sho-machi, 770-8505 Tokushima, Japan. sato@ph.tokushima-u.ac.jp.
authors.forename: Youichi
authors.initials: Y
authors.lastname: Sato
authors.affiliation: Department of Human Genetics Public Health, School of Medicine, The University of Tokushima, 3-18-15 Kuramoto-cho, 770-8503 Tokushima, Japan. nakahori@basic.med.tokushima-u.ac.jp.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Nakahori
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, 464-8603 Nagoya, Japan. babaymtt@apchem.nagoya-u.ac.jp.|ImPACT Research Center for Advanced Nanobiodevices., Nagoya University, Furo-cho, Chikusa-ku, 464-8603 Nagoya, Japan. babaymtt@apchem.nagoya-u.ac.jp.|Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Hayashi-cho 2217-14, 761-0395 Takamatsu, Japan. babaymtt@apchem.nagoya-u.ac.jp.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Baba
keywords: DNA; anomalous separation; microchip electrophoresis