title: Plant-Mediated Synthesis of Silver Nanoparticles and Their Stabilization by Wet Stirred Media Milling.
abstract: Within this study, a stable nanosuspension of silver nanoparticles (Ag NPs) was prepared using a two-step synthesis and stabilization approach. The Ag NPs were synthesized from a silver nitrate solution using the Origanum vulgare L. plant extract as the reducing agent. The formation of nanoparticles was finished upon 15 min, and subsequently, stabilization by polyvinylpyrrolidone (PVP) using wet stirred media milling was applied. UV-Vis spectra have shown a maximum at 445 nm, corresponding to the formation of spherical Ag NPs. Infrared spectroscopy was used to examine the interaction between Ag NPs and the capping agents. TEM study has shown the formation of Ag NPs with two different average sizes (38 ± 10 nm and 7 ± 3 nm) after the plant-mediated synthesis, both randomly distributed within the organic matrix. During milling in PVP, the clusters of Ag NPs were destroyed, the Ag NPs were fractionized and embedded in PVP. The nanosuspensions of PVP-capped Ag NPs were stable for more than 26 weeks, whereas for the non-stabilized nanosuspensions, only short-term stability for about 1 week was documented.
authors.affiliation: Department of Mechanochemistry, Institute of Geotechnics, Slovak Academy of Sciences, Watsonova 45, Košice, 04001, Slovakia.
authors.forename: Matej
authors.initials: M
authors.lastname: Baláž
authors.affiliation: Department of Pharmacognosy and Botany, University of Veterinary Medicine and Pharmacy, Komenského 73, Košice, 04181, Slovakia.
authors.forename: Ľudmila
authors.initials: Ľ
authors.lastname: Balážová
authors.affiliation: Department of Nanostructured Materials, Jožef Stefan Institute, Jamova cesta 39, Ljubljana, 1000, Slovenia.
authors.forename: Nina
authors.initials: N
authors.lastname: Daneu
authors.affiliation: Department of Mechanochemistry, Institute of Geotechnics, Slovak Academy of Sciences, Watsonova 45, Košice, 04001, Slovakia.
authors.forename: Erika
authors.initials: E
authors.lastname: Dutková
authors.affiliation: Department of Pharmacognosy and Botany, University of Veterinary Medicine and Pharmacy, Komenského 73, Košice, 04181, Slovakia.
authors.forename: Miriama
authors.initials: M
authors.lastname: Balážová
authors.affiliation: Department of Mechanochemistry, Institute of Geotechnics, Slovak Academy of Sciences, Watsonova 45, Košice, 04001, Slovakia.
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Bujňáková
authors.affiliation: Ivan Franko National University of Lviv, Tarnavskogo 107, Lviv, 79017, Ukraine. yashpotyuk@gmail.com.|CITNSEK, Faculty of Mathematics and Natural Sciences, University of Rzeszow, Pigonia 1, Rzeszow, 35-958, Poland. yashpotyuk@gmail.com.
authors.forename: Yaroslav
authors.initials: Y
authors.lastname: Shpotyuk
keywords: Mechanochemistry; Origanum vulgare L.; Plant-mediated synthesis; Polyvinylpyrrolidone; Silver NPs