title: Modulation of antiphospholipid antibodies-induced trophoblast damage by different drugs used to prevent pregnancy morbidity associated with antiphospholipid syndrome.
abstract: Women with antiphospholipid antibodies (aPLs) present a risk of pregnancy morbidity (PM), vascular thrombosis (VT), or both (PM/VT). aPLs affect trophoblast function, and the aim of this study was to determine the modulation of this aPL-induced damage by different drugs.
  
  
  
  IgG was obtained from women with PM and PM/VT positive to aPLs. Binding of IgG to trophoblastic cells, proliferation, mitochondrial membrane integrity, and trophoblast invasion were assessed. The effect of enoxaparin, aspirin, and aspirin-triggered lipoxin (ATL) were evaluated as well as signal transducer and activator of transcription 3 (STAT3) phosphorylation.
  
  
  
  IgG from women with aPLs strongly binds to trophoblastic cells. Integrity of mitochondrial membrane was reduced, and proliferation was increased by IgG-PM/VT. Both IgG-PM and IgG-PM/VT decreased trophoblast invasion, which was restored by enoxaparin, aspirin, and ATL. IgG-PM triggered reduction in STAT3 phosphorylation.
  
  
  
  Some drugs used to prevent aPL-induced PM modulated the alteration of trophoblast function.
authors.affiliation: Reproduction Group, School of Medicine, Universidad de Antioquia, Medellín, Colombia.
authors.forename: Angela M
authors.identifier: 0000-0002-9056-9764
authors.initials: AM
authors.lastname: Alvarez
authors.affiliation: Physiology and Biochemistry Department, School of Medicine, Universidad de Antioquia, Medellín, Colombia.
authors.forename: Norman
authors.initials: N
authors.lastname: Balcázar
authors.affiliation: Biomedical Research Center, Universidad de Valparaíso, Valparaíso, Chile.
authors.forename: Sebastián
authors.initials: S
authors.lastname: San Martín
authors.affiliation: Placenta Laboratory, Department of Obstetrics, Jena University Hospital, Jena, Germany.
authors.forename: Udo R
authors.initials: UR
authors.lastname: Markert
authors.affiliation: Reproduction Group, School of Medicine, Universidad de Antioquia, Medellín, Colombia.
authors.forename: Angela P
authors.initials: AP
authors.lastname: Cadavid
mesh_terms: D000328:Adult; D017152:Antibodies, Antiphospholipid / Q000276:immunology*; D000925:Anticoagulants / Q000494:pharmacology; D016736:Antiphospholipid Syndrome / Q000150:complications* / Q000276:immunology; D001241:Aspirin / Q000494:pharmacology; D015153:Blotting, Western; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007150:Immunohistochemistry; D044045:Lipoxins / Q000494:pharmacology; D011247:Pregnancy; D011248:Pregnancy Complications / Q000276:immunology*; D014327:Trophoblasts / Q000187:drug effects / Q000276:immunology* / Q000473:pathology
chemical_list: D017152:Antibodies, Antiphospholipid; D000925:Anticoagulants; D007074:Immunoglobulin G; D044045:Lipoxins; D001241:Aspirin
keywords: 15-epi-lipoxin A4; STAT3; placental development; pregnancy morbidity