Fluorescent "on-off-on" switching sensor based on CdTe quantum dots coupled with multiwalled carbon nanotubes@graphene oxide nanoribbons for simultaneous monitoring of dual foreign DNAs in transgenic soybean.
With the increasing concern of potential health and environmental risk, it is essential to develop reliable methods for transgenic soybean detection. Herein, a simple, sensitive and selective assay was constructed based on homogeneous fluorescence resonance energy transfer (FRET) between CdTe quantum dots (QDs) and multiwalled carbon nanotubes@graphene oxide nanoribbons (MWCNTs@GONRs) to form the fluorescent "on-off-on" switching for simultaneous monitoring dual target DNAs of promoter cauliflower mosaic virus 35s (P35s) and terminator nopaline synthase (TNOS) from transgenic soybean. The capture DNAs were immobilized with corresponding QDs to obtain strong fluorescent signals (turning on). The strong π-π stacking interaction between single-stranded DNA (ssDNA) probes and MWCNTs@GONRs led to minimal background fluorescence due to the FRET process (turning off). The targets of P35s and TNOS were recognized by dual fluorescent probes to form double-stranded DNA (dsDNA) through the specific hybridization between target DNAs and ssDNA probes. And the dsDNA were released from the surface of MWCNTs@GONRs, which leaded the dual fluorescent probes to generate the strong fluorescent emissions (turning on). Therefore, this proposed homogeneous assay can be achieved to detect P35s and TNOS simultaneously by monitoring the relevant fluorescent emissions. Moreover, this assay can distinguish complementary and mismatched nucleic acid sequences with high sensitivity. The constructed approach has the potential to be a tool for daily detection of genetically modified organism with the merits of feasibility and reliability.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Yaqi
Y
Li
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Li
L
Sun
School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Jing
J
Qian
School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Lingliang
L
Long
School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Henan
H
Li
School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Qian
Q
Liu
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China. Electronic address: jrcai@ujs.edu.cn.
Jianrong
J
Cai
School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China. Electronic address: wangkun@ujs.edu.cn.
Kun
K
Wang
D000594:Amino Acid Oxidoreductases / Q000235:genetics; D015374:Biosensing Techniques / Q000379:methods; D019187:Cadmium Compounds / Q000737:chemistry*; D017796:Caulimovirus / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D061925:Immobilized Nucleic Acids / Q000737:chemistry / Q000235:genetics; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D010087:Oxides / Q000737:chemistry; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000821:virology; D045663:Quantum Dots / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D013025:Glycine max / Q000235:genetics* / Q000821:virology; D013691:Tellurium / Q000737:chemistry*
D019187:Cadmium Compounds; D018744:DNA, Plant; D004279:DNA, Viral; D061925:Immobilized Nucleic Acids; D037742:Nanotubes, Carbon; D010087:Oxides; D010940:Plant Proteins; D006108:Graphite; D000594:Amino Acid Oxidoreductases; C021883:nopaline synthase; D013691:Tellurium; C028337:cadmium telluride
Fluorescence resonance energy transfer; Multiwalled carbon nanotubes@graphene oxide nanoribbons; Quantum dots; Transgenic soybean; “On-Off-On” switching