Forensic SNP Genotyping using Nanopore MinION Sequencing.
One of the latest developments in next generation sequencing is the Oxford Nanopore Technologies' (ONT) MinION nanopore sequencer. We studied the applicability of this system to perform forensic genotyping of the forensic female DNA standard 9947 A using the 52 SNP-plex assay developed by the SNPforID consortium. All but one of the loci were correctly genotyped. Several SNP loci were identified as problematic for correct and robust genotyping using nanopore sequencing. All these loci contained homopolymers in the sequence flanking the forensic SNP and most of them were already reported as problematic in studies using other sequencing technologies. When these problematic loci are avoided, correct forensic genotyping using nanopore sequencing is technically feasible.
Laboratory of Pharmaceutical Biotechnology, Ghent University Ghent, Belgium.
Senne
S
Cornelis
Laboratory of Pharmaceutical Biotechnology, Ghent University Ghent, Belgium.
Yannick
Y
Gansemans
Laboratory of Pharmaceutical Biotechnology, Ghent University Ghent, Belgium.
Lieselot
L
Deleye
Laboratory of Pharmaceutical Biotechnology, Ghent University Ghent, Belgium.
Dieter
D
Deforce
Laboratory of Pharmaceutical Biotechnology, Ghent University Ghent, Belgium.
Filip
F
Van Nieuwerburgh
D000483:Alleles; D053803:Forensic Genetics / Q000379:methods; D005787:Gene Frequency; D056426:Genetic Loci; D060005:Genotyping Techniques; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D018895:Microsatellite Repeats; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA