Gene duplication can impart fragility, not robustness, in the yeast protein interaction network.
The maintenance of duplicated genes is thought to protect cells from genetic perturbations, but the molecular basis of this robustness is largely unknown. By measuring the interaction of yeast proteins with their partners in wild-type cells and in cells lacking a paralog, we found that 22 out of 56 paralog pairs compensate for the lost interactions. An equivalent number of pairs exhibit the opposite behavior and require each other's presence for maintaining their interactions. These dependent paralogs generally interact physically, regulate each other's abundance, and derive from ancestral self-interacting proteins. This reveals that gene duplication may actually increase mutational fragility instead of robustness in a large number of cases.
Département de Biologie, Université Laval, Québec, QC, Canada.|The Quebec Network for Research on Protein Function, Engineering, and Applications, Université Laval, Québec, QC, Canada.|Institut de Biologie Intégrative et des Systèmes, Université Laval, Québec, QC, Canada.|EMBL/CRG Systems Biology Research Unit, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology (BIST), Doctor Aiguader 88, 08003 Barcelona, Spain.|Universitat Pompeu Fabra (UPF), 08003 Barcelona, Spain.
Guillaume
0000-0001-9153-4523
G
Diss
Département de Biologie, Université Laval, Québec, QC, Canada.|The Quebec Network for Research on Protein Function, Engineering, and Applications, Université Laval, Québec, QC, Canada.|Institut de Biologie Intégrative et des Systèmes, Université Laval, Québec, QC, Canada.
Isabelle
I
Gagnon-Arsenault
Département de Biologie, Université Laval, Québec, QC, Canada.|The Quebec Network for Research on Protein Function, Engineering, and Applications, Université Laval, Québec, QC, Canada.|Institut de Biologie Intégrative et des Systèmes, Université Laval, Québec, QC, Canada.
Anne-Marie
0000-0002-8656-4127
AM
Dion-Coté
Département de Biologie, Université Laval, Québec, QC, Canada.|The Quebec Network for Research on Protein Function, Engineering, and Applications, Université Laval, Québec, QC, Canada.|Institut de Biologie Intégrative et des Systèmes, Université Laval, Québec, QC, Canada.
Hélène
H
Vignaud
Département de Biologie, Université Laval, Québec, QC, Canada.|The Quebec Network for Research on Protein Function, Engineering, and Applications, Université Laval, Québec, QC, Canada.|Institut de Biologie Intégrative et des Systèmes, Université Laval, Québec, QC, Canada.|Laboratorio Nacional de Genómica para la Biodiversidad (LANGEBIO), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Irapuato, Guanajuato, Mexico.
Diana I
DI
Ascencio
Département de Biologie, Université Laval, Québec, QC, Canada.|The Quebec Network for Research on Protein Function, Engineering, and Applications, Université Laval, Québec, QC, Canada.|Institut de Biologie Intégrative et des Systèmes, Université Laval, Québec, QC, Canada.
Caroline M
CM
Berger
Département de Biologie, Université Laval, Québec, QC, Canada. christian.landry@bio.ulaval.ca.|The Quebec Network for Research on Protein Function, Engineering, and Applications, Université Laval, Québec, QC, Canada.|Institut de Biologie Intégrative et des Systèmes, Université Laval, Québec, QC, Canada.
Christian R
0000-0003-3028-6866
CR
Landry
D019143:Evolution, Molecular; D020440:Gene Duplication; D020131:Genes, Duplicate; D060066:Protein Interaction Maps / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
D029701:Saccharomyces cerevisiae Proteins