Apigenin alleviates STZ-induced diabetic cardiomyopathy.
Apigenin is an important component of fruits and vegetables in human daily diets. Several cellular and animal models have been performed to demonstrate its anti-oxidant and anti-inflammatory bioactivities. However, the cardioprotective effects of apigenin in diabetic cardiomyopathy (DCM) remain unclear. In this study, we intended to explore the roles of apigenin in cardiac remodeling of DCM. Male C57BL/6 J mice were treated with streptozotocin (STZ, 50 mg/kg) for 5 consecutive days to induce DCM. The echocardiography and catheter-based measurements of hemodynamic parameters were performed to evaluate the cardiac function. Paraffin slices of harvested hearts were prepared for histological pathological analysis and TUNEL assay. Oxidative assay kits were used to detect Glutathione Peroxidase (GPx), Lipid Peroxidation Malondialdehyde (MDA), and Superoxide Dismutase (SOD). Western blot and real-time PCR were used for accessing the expressions of protein and mRNA. Diabetes mellitus exacerbated the cardiac dysfunction, fibrosis, and overaccumulation of 4-hydroxynonenal accompanying with down-regulation of Bcl2, GPx, and SOD, up-regulation of MDA, cleaved caspase3, and pro-apoptotic protein Bax, and contribution to the translocation of NF-κB. All these pathological changes could be effectively blunted by treatment of apigenin in vivo. Finally, H9c2 treated with high glucose or apigenin was used for further investigation of these effects in vitro; what is more, we also compared the effects between apigenin and Resveratrol in in vitro experiments. Our experiments have demonstrated that apigenin may be a potential drug for diabetic patients suffering from DCM.
Department of Cardiology, Renmin Hospital of Wuhan University, Jiefang Road 238, Wuhan, 430060, China.|Cardiovascular Research Institute of Wuhan University, Wuhan, China.|Hubei Key Laboratory of Cardiology, Wuhan, China.
Huang-Jun
HJ
Liu
Department of Cardiology, Renmin Hospital of Wuhan University, Jiefang Road 238, Wuhan, 430060, China.
Yun-Lin
YL
Fan
Department of Cardiology, Renmin Hospital of Wuhan University, Jiefang Road 238, Wuhan, 430060, China.|Cardiovascular Research Institute of Wuhan University, Wuhan, China.|Hubei Key Laboratory of Cardiology, Wuhan, China.
Hai-Han
HH
Liao
Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Yuan
Y
Liu
College of Pharmacy, Hubei University of Science and Technology, Xianning, China.
Si
S
Chen
Department of Cardiology, Renmin Hospital of Wuhan University, Jiefang Road 238, Wuhan, 430060, China.|Cardiovascular Research Institute of Wuhan University, Wuhan, China.|Hubei Key Laboratory of Cardiology, Wuhan, China.
Zhen-Guo
ZG
Ma
Department of Cardiology, Renmin Hospital of Wuhan University, Jiefang Road 238, Wuhan, 430060, China.|Cardiovascular Research Institute of Wuhan University, Wuhan, China.|Hubei Key Laboratory of Cardiology, Wuhan, China.
Ning
N
Zhang
Department of Cardiology, Renmin Hospital of Wuhan University, Jiefang Road 238, Wuhan, 430060, China.|Cardiovascular Research Institute of Wuhan University, Wuhan, China.|Hubei Key Laboratory of Cardiology, Wuhan, China.
Zheng
Z
Yang
Department of Cardiology, Renmin Hospital of Wuhan University, Jiefang Road 238, Wuhan, 430060, China.|Cardiovascular Research Institute of Wuhan University, Wuhan, China.|Hubei Key Laboratory of Cardiology, Wuhan, China.
Wei
W
Deng
Department of Cardiology, Renmin Hospital of Wuhan University, Jiefang Road 238, Wuhan, 430060, China. qztang@whu.edu.cn.|Cardiovascular Research Institute of Wuhan University, Wuhan, China. qztang@whu.edu.cn.|Hubei Key Laboratory of Cardiology, Wuhan, China. qztang@whu.edu.cn.
Qi-Zhu
QZ
Tang
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000494:pharmacology; D047310:Apigenin / Q000008:administration & dosage* / Q000494:pharmacology; D002316:Cardiotonic Agents / Q000008:administration & dosage* / Q000494:pharmacology; D002460:Cell Line; D058065:Diabetic Cardiomyopathies / Q000139:chemically induced / Q000188:drug therapy*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage / Q000494:pharmacology; D013311:Streptozocin / Q000009:adverse effects*
D000975:Antioxidants; D002316:Cardiotonic Agents; D013267:Stilbenes; D013311:Streptozocin; D047310:Apigenin; D000077185:Resveratrol
Apigenin; Apoptosis; Diabetic cardiomyopathy; Inflammation; Oxidative stress