Investigation of AGID and two commercial ELISAs for the detection of Bovine viral diarrhea virus-specific antibodies in sheep serum.
Effective control and the eventual eradication of Bovine viral diarrhea virus (BVDV) from cattle populations depend on the accurate identification of infected animals. Although typically a disease agent of cattle, BVDV is known to infect a wide variety of nonbovine species, including sheep. However, validation of serologic tests in these nonbovine species, particularly sheep, is lacking. We analyzed 99 sheep sera (57 samples from Pestivirus-naive sheep, and 42 samples from BVDV-inoculated sheep) in order to investigate 3 serologic tests: the agarose gel immunodiffusion (AGID) and 2 commercial enzyme-linked immunosorbent assays (ELISAs) for detection of BVDV antibodies. At the manufacturer's cutoff thresholds, the AGID performed with 95.2% diagnostic sensitivity; ELISA-A performed with sensitivity of 90.5% and ELISA-B with 69.1%. All 3 tests performed with 100% diagnostic specificity. Two-graph receiver operating characteristic analysis showed that performance characteristics were optimized, such that both diagnostic sensitivity and diagnostic specificity were >95% for both ELISAs, if the thresholds were altered to 34.9% inhibition for ELISA-A and 63.5 signal-to-noise ratio for ELISA-B.
School of Animal and Veterinary Sciences, Roseworthy Campus, University of Adelaide, Roseworthy, Australia (Evans, Lanyon).|School of Veterinary Medicine, City University of Hong Kong, Hong Kong (Reichel).
Caitlin A
CA
Evans
School of Animal and Veterinary Sciences, Roseworthy Campus, University of Adelaide, Roseworthy, Australia (Evans, Lanyon).|School of Veterinary Medicine, City University of Hong Kong, Hong Kong (Reichel).
Sasha R
SR
Lanyon
School of Animal and Veterinary Sciences, Roseworthy Campus, University of Adelaide, Roseworthy, Australia (Evans, Lanyon).|School of Veterinary Medicine, City University of Hong Kong, Hong Kong (Reichel).
Michael P
MP
Reichel
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000517:prevention & control* / Q000821:virology; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005779:Immunodiffusion / Q000662:veterinary; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000097:blood / Q000517:prevention & control* / Q000821:virology
D000914:Antibodies, Viral
Bovine viral diarrhea virus; serologic tests; sheep; test validation