Double-Immunodiffusion Assay for Detecting Specific Antibodies (Ouchterlony).
The method first described by Ouchterlony in 1948 is a classic and simple technique that permits evaluation and comparison of antibodies in animal or human sera directed against protein or complex carbohydrate antigens. It is a low-tech procedure that may provide information on the relative quantity of antibody activity and the nature of the antigenic epitopes in different preparations. © 2017 by John Wiley & Sons, Inc.
Cell Signaling Technologies, Danvers, Massachusetts.
Peter
P
Hornbeck
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology*; D006801:Humans; D005779:Immunodiffusion / Q000379:methods*
D000906:Antibodies
agar; antibody assay; antigen; double-diffusion; gel