Bradyrhizobium centrolobii and Bradyrhizobium macuxiense sp. nov. isolated from Centrolobium paraense grown in soil of Amazonia, Brazil.
Thirteen Gram-negative, aerobic, motile with polar flagella, rod-shaped bacteria were isolated from root nodules of Centrolobium paraense Tul. grown in soils from the Amazon region of Brazil. Growth of strains was observed at temperature range 20-36 °C (optimal 28 °C), pH ranges 5-11 (optimal 6.0-7.0), and 0.1-0.5%NaCl (optimal 0.1-0.3%). Analysis of 16S rRNA gene placed the strains into two groups within Bradyrhizobium. Closest neighbouring species (98.8%) for group I was B. neotropicale while for group II were 12 species with more than 99% of similarity. Multi-locus sequence analysis (MLSA) with dnaK, glnII, recA, and rpoB confirmed B. neotropicale BR 10247Tas the closest type strain for the group I and B. elkanii USDA 76Tand B. pachyrhizi PAC 48Tfor group II. Average Nucleotide Identity (ANI) differentiated group I from the B. neotropicale BR 10247T(79.6%) and group II from B. elkanii USDA 76Tand B. pachyrhizi PAC 48T(88.1% and 87.9%, respectively). Fatty acid profiles [majority C16:0and Summed feature 8 (18:1ω6c/18:1ω7c) for both groups], DNA G + C content, and carbon compound utilization supported the placement of the novel strains in the genus Bradyrhizobium. Gene nodC and nifH of the new strains have in general low similarity with other Bradyrhizobium species. Both groups nodulated plants from the tribes Crotalarieae, Dalbergiae, Genisteae, and Phaseoleae. Based on the presented data, two novel species which the names Bradyrhizobium centrolobii and Bradyrhizobium macuxiense are proposed, with BR 10245T(=HAMBI 3597T) and BR 10303T(=HAMBI 3602T) as the respective-type strains.
Universidade Federal Rural do Rio de Janeiro, Rodovia BR 465 km 07, Seropédica, Rio De Janeiro, 23890-000, Brazil.
Daniele C
DC
Michel
Universidade Federal Rural do Rio de Janeiro, Rodovia BR 465 km 07, Seropédica, Rio De Janeiro, 23890-000, Brazil.
Samuel R
SR
Passos
Embrapa Agrobiologia, Rodovia BR 465 km 07, Seropédica, Rio De Janeiro, 23891-000, Brazil.
Jean L
JL
Simões-Araujo
Universidade Federal Rural do Rio de Janeiro, Rodovia BR 465 km 07, Seropédica, Rio De Janeiro, 23890-000, Brazil.
Alexandre C
AC
Baraúna
Embrapa Roraima, Rodovia BR 174 km 08, Boa Vista, Roraima, 69301-970, Brazil.
Krisle
K
da Silva
Embrapa Meio Ambiente, Rodovia SP 340 km 127, 5, Jaguariúna, São Paulo, 13820-000, Brazil.
Marcia M
MM
Parma
Embrapa Meio Ambiente, Rodovia SP 340 km 127, 5, Jaguariúna, São Paulo, 13820-000, Brazil.
Itamar S
IS
Melo
Centre for Rhizobium Studies, Murdoch University, 90 South Street, Murdoch, WA, 6150, Australia.
Sofie E
SE
De Meyer
Centre for Rhizobium Studies, Murdoch University, 90 South Street, Murdoch, WA, 6150, Australia.
Graham
G
O'Hara
Embrapa Agrobiologia, Rodovia BR 465 km 07, Seropédica, Rio De Janeiro, 23891-000, Brazil. jerri.zilli@embrapa.br.
Jerri E
JE
Zilli
D001426:Bacterial Proteins / Q000235:genetics; D001482:Base Composition / Q000235:genetics; D020369:Bradyrhizobium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001938:Brazil; D004269:DNA, Bacterial / Q000235:genetics; D007887:Fabaceae / Q000382:microbiology*; D005227:Fatty Acids / Q000737:chemistry; D058885:Multilocus Sequence Typing; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics; D009586:Nitrogen Fixation / Q000235:genetics / Q000502:physiology; D009693:Nucleic Acid Hybridization; D010088:Oxidoreductases / Q000235:genetics; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D053204:Root Nodules, Plant / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012987:Soil; D012988:Soil Microbiology
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012987:Soil; D010088:Oxidoreductases; C029130:nitrogenase reductase; D017351:N-Acetylglucosaminyltransferases; C060771:NodC protein, Rhizobiales
Biological nitrogen fixation; Bradyrhizobium centrolobii and Bradyrhizobium macuxiense; Legumes; Nodulation