Characterisation of new gated optical image intensifiers for fluorescence lifetime imaging.
We report the characterisation of gated optical image intensifiers for fluorescence lifetime imaging, evaluating the performance of several different prototypes that culminate in a new design that provides improved spatial resolution conferred by the addition of a magnetic field to reduce the lateral spread of photoelectrons on their path between the photocathode and microchannel plate, and higher signal to noise ratio conferred by longer time gates. We also present a methodology to compare these systems and their capabilities, including the quantitative readouts of Förster resonant energy transfer.
Photonics Group, Department of Physics, Imperial College London, Prince Consort Road, London SW7 2BW, United Kingdom.
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0000000199006610
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Sparks
Photonics Group, Department of Physics, Imperial College London, Prince Consort Road, London SW7 2BW, United Kingdom.
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0000000284151820
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Görlitz
Photonics Group, Department of Physics, Imperial College London, Prince Consort Road, London SW7 2BW, United Kingdom.
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Kelly
Photonics Group, Department of Physics, Imperial College London, Prince Consort Road, London SW7 2BW, United Kingdom.
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0000000252537147
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Warren
Kentech Instruments Ltd., Howbery Park, Wallingford OX10 8BD, United Kingdom.
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Kellett
Photonics Group, Department of Physics, Imperial College London, Prince Consort Road, London SW7 2BW, United Kingdom.
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Garcia
Kentech Instruments Ltd., Howbery Park, Wallingford OX10 8BD, United Kingdom.
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0000000247034289
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Dymoke-Bradshaw
Kentech Instruments Ltd., Howbery Park, Wallingford OX10 8BD, United Kingdom.
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0000000256257214
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Hares
Photonics Group, Department of Physics, Imperial College London, Prince Consort Road, London SW7 2BW, United Kingdom.
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Neil
Photonics Group, Department of Physics, Imperial College London, Prince Consort Road, London SW7 2BW, United Kingdom.
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Dunsby
Photonics Group, Department of Physics, Imperial College London, Prince Consort Road, London SW7 2BW, United Kingdom.
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French