Effects of nonionic surfactants on pellet formation and the production of β-fructofuranosidases from Aspergillus oryzae KB.
Aspergillus oryzae KB produces two β-fructofuranosidases (F1 and F2). F1 has high transferring activity and produces fructooligosaccharides from sucrose. Mycelial growth pellets were altered by the addition of Tween 20, 40 and 80 (HLB=16.7, 15.6 and 15.0, respectively) in liquid medium cultures to form small spherical pellets. The particle size of the pellets decreased with the HLB value, which corresponds to an increase in surfactant hydrophobicity. Selective F1 production and pellet size were maximized using Tween 20. Adding polyoxyethylene oleyl ethers (POEs) with various degrees of polymerization (2, 7, 10, 20 and 50: HLB=7.7, 10.7, 14.7, 17.2 and 18.2, respectively) was investigated. A minimum mean particle size was obtained using a POE with DP=10, HLB=14.7. The POE surfactants had little effect on the selective production of F1. The formation of filamentous pellets depended on the surfactant HLB value, and F1 enzymes were produced most efficiently using Tween 20.
Department of Marine Biotechnology, Fukuyama University, 1-985 Sanzo, Higashimura-cho, Fukuyama, Hiroshima 729-0292, Japan; Department of Life and Nutritional Science, Fukuyama University, 1-985 Sanzo, Higashimura-cho, Fukuyama, Hiroshima 729-0292, Japan. Electronic address: kurakake@fubac.fukuyama-u.ac.jp.
Masahiro
M
Kurakake
Department of Life and Nutritional Science, Fukuyama University, 1-985 Sanzo, Higashimura-cho, Fukuyama, Hiroshima 729-0292, Japan.
Saya
S
Hirotsu
Department of Life and Nutritional Science, Fukuyama University, 1-985 Sanzo, Higashimura-cho, Fukuyama, Hiroshima 729-0292, Japan.
Miyuki
M
Shibata
Department of Life and Nutritional Science, Fukuyama University, 1-985 Sanzo, Higashimura-cho, Fukuyama, Hiroshima 729-0292, Japan.
Yuta
Y
Takenaka
Department of Life and Nutritional Science, Fukuyama University, 1-985 Sanzo, Higashimura-cho, Fukuyama, Hiroshima 729-0292, Japan.
Taiki
T
Kamioka
Department of Life and Nutritional Science, Fukuyama University, 1-985 Sanzo, Higashimura-cho, Fukuyama, Hiroshima 729-0292, Japan.
Tatsuya
T
Sakamoto
D001236:Aspergillus oryzae / Q000201:enzymology*; D057927:Hydrophobic and Hydrophilic Interactions / Q000187:drug effects; D009844:Oligosaccharides / Q000096:biosynthesis / Q000302:isolation & purification; D010316:Particle Size; D010938:Plant Oils / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D011136:Polysorbates / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology*; D043324:beta-Fructofuranosidase / Q000096:biosynthesis* / Q000302:isolation & purification
D009844:Oligosaccharides; D010938:Plant Oils; D011136:Polysorbates; D013501:Surface-Active Agents; C116580:fructooligosaccharide; D011092:Polyethylene Glycols; C033084:polyethylene glycol oleyl ether; D043324:beta-Fructofuranosidase
Aspergillus; Fructosyltransferase; Pellet; Surfactant; Transfructosylation; Tween