Evaluation of Serum-Free, Xeno-Free Cryopreservation Solutions for Human Adipose-Derived Mesenchymal Stem Cells.
Adipose-derived mesenchymal stem cells (ASCs) have the potential to differentiate into cells of mesodermal origin, such as osteoblasts, adipocytes, myocytes, and chondrocytes, and cryopreservation is currently performed as a routine method for preserving ASCs to safely acquire large numbers of cells. For clinical application of ASCs, serum-free, xeno-free cryopreservation solutions should be used. This study determined the viability and adipo-osteogenic potential of cryopreserved ASCs using four cryopreservation solutions: 10% DMSO, Cell Banker 2 (serum free), Stem Cell Banker (=Cell Banker 3: serum free, xeno free), and TC protector (serum free, xeno free). The viability of the cryopreserved ASCs was over 80% with all cryopreservation solutions. No difference in the adipo-osteogenic potential was found between the cells that did or did not undergo cryopreservation in these cryopreservation solutions. These data suggest that Cell Banker 3 and TC protector are comparable with 10% DMSO and Cell Banker 2 for ASCs, and cryopreserved as well as noncryopreserved ASCs could be applied for regenerative medicine.
Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus , Okinawa , Japan.
Chika
C
Miyagi-Shiohira
† Okayama Saidaiji Hospital , Okayama , Japan.
Naoya
N
Kobayashi
‡ Division of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University , Niigata , Japan.
Issei
I
Saitoh
§ Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
Masami
M
Watanabe
¶ Department of Socio-environmental Design, Hiroshima International University , Hiroshima , Japan.
Yasufumi
Y
Noguchi
# Department of Molecular and Cellular Physiology, Graduate School of Medicine, University of the Ryukyus , Okinawa , Japan.
Masayuki
M
Matsushita
Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus , Okinawa , Japan.
Hirofumi
H
Noguchi
Adipose-derived mesenchymal stem cells (ASCs); Cell Banker 3; Cryopreservation; Dimethyl sulfoxide (DMSO); TC protector