Time course effect of lipopolysaccharide on Toll-like receptors expression and steroidogenesis in the Chinese goose ovary.
The ovary of Chinese goose is easily infected by microorganisms because of the mating behaviour in water, which causes decreased laying performance. This study investigated the time course effect of lipopolysaccharide (LPS) on the steroidogenesis and mRNA expression of Toll-like receptors (TLRs), a class of key pattern recognition receptor, in the breeding goose ovary. The laying geese were treated intravenously with LPS for 0, 6, 12, 24 and 36 h, and all birds were slaughtered approximately 8 h after oviposition. The expression levels ofTLRs in the white and yellowish follicles, and granulosa and theca layers of hierarchical follicles were examined by real-time PCR. All 10 members of avianTLRfamily were differentially expressed among the different follicular tissues. Moreover, at 24 and 36 h after LPS treatment, the hierarchical follicle morphological structure was altered, but the expression levels ofTLRs were still higher than the control. Furthermore, during LPS treatment period, the expression pattern ofTLRs2Aand4genes was similar to that ofTLR15in the white follicles,TLRs1B,5and15in the yellowish follicles,TLRs7and15in the granulosa layer, andTLRs1A,2B,3,7and15in the theca layer, which had a negative correlation with the kinetics of plasma P4 and E2 concentrations. In conclusion, the mechanism by which pathogen infection inhibited goose follicular growth and further decreased egg production may involve a gradually enhanced inflammatory response and reduced endocrine function. This may be due to stimulatedTLRs in the ovary.
Institute of Animal ScienceLaboratory of Animal Improvement and Reproduction, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
ShiJia
S
Ying
Institute of Animal ScienceLaboratory of Animal Improvement and Reproduction, Jiangsu Academy of Agricultural Sciences, Nanjing, China.|College of Animal Science and Veterinary MedicineShandong Agricultural University, Taian, Shandong, China.
JiaJia
J
Guo
Institute of Animal ScienceLaboratory of Animal Improvement and Reproduction, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
ZiChun
Z
Dai
Institute of Animal ScienceLaboratory of Animal Improvement and Reproduction, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
HuanXi
H
Zhu
Institute of Animal ScienceLaboratory of Animal Improvement and Reproduction, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
JianNing
J
Yu
College of Animal Science and Veterinary MedicineShandong Agricultural University, Taian, Shandong, China.
WeiMing
W
Ma
College of Animal Science and TechnologyNanjing Agricultural University, Nanjing, Jiangsu, China.
JiaYi
J
Li
Institute of Animal ScienceLaboratory of Animal Improvement and Reproduction, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
Muhammad Faheem
MF
Akhtar
Institute of Animal ScienceLaboratory of Animal Improvement and Reproduction, Jiangsu Academy of Agricultural Sciences, Nanjing, China zdshi@jaas.ac.cn.
ZhenDan
Z
Shi
D000818:Animals; D005260:Female; D005777:Geese; D005786:Gene Expression Regulation / Q000187:drug effects*; D012739:Gonadal Steroid Hormones / Q000378:metabolism*; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D006080:Ovarian Follicle / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010053:Ovary / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D013799:Theca Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D051193:Toll-Like Receptors / Q000235:genetics / Q000378:metabolism*
D012739:Gonadal Steroid Hormones; D008070:Lipopolysaccharides; D051193:Toll-Like Receptors