Bacterial communities in sediment of a Mediterranean marine protected area.
Biodiversity is crucial in preservation of ecosystems, and bacterial communities play an indispensable role for the functioning of marine ecosystems. The Mediterranean marine protected area (MPA) "Capo Gallo-Isola delle Femmine" was instituted to preserve marine biodiversity. The bacterial diversity associated with MPA sediment was compared with that from sediment of an adjacent harbour exposed to intense nautical traffic. The MPA sediment showed higher diversity with respect to the impacted site. A 16S rDNA clone library of the MPA sediment allowed the identification of 7 phyla: Proteobacteria (78%), Firmicutes (11%), Acidobacteria (3%), Actinobacteria (3%), Bacteroidetes (2%), Planctomycetes (2%), and Cyanobacteria (1%). Analysis of the hydrocarbon (HC)-degrading bacteria was performed using enrichment cultures. Most of the MPA sediment isolates were affiliated with Gram-positive G+C rich bacteria, whereas the majority of taxa in the harbour sediment clustered with Alpha- and Gammaproteobacteria; no Gram-positive HC degraders were isolated from the harbour sediment. Our results show that protection probably has an influence on bacterial diversity, and suggest the importance of monitoring the effects of protection at microbial level as well. This study creates a baseline of data that can be used to assess changes over time in bacterial communities associated with a Mediterranean MPA.
a Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF), Università degli Studi di Palermo, Viale delle Scienze Ed.16 - 90128 Palermo, Italia.
Valentina
V
Catania
b Dipartimento di Scienze della Terra e del Mare (DISTEM), Università degli Studi di Palermo, Viale delle Scienze Ed.16 - 90128 Palermo, Italia.
Gianluca
G
Sarà
c Dipartimento di Scienze Agrarie e Forestali (SAF), Università degli Studi di Palermo, Viale delle Scienze Ed.4 - 90128 Palermo, Italia.
Luca
L
Settanni
a Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF), Università degli Studi di Palermo, Viale delle Scienze Ed.16 - 90128 Palermo, Italia.
Paola
P
Quatrini
D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D044822:Biodiversity; D017753:Ecosystem; D019015:Geologic Sediments / Q000382:microbiology*; D006838:Hydrocarbons / Q000378:metabolism; D010802:Phylogeny
D006838:Hydrocarbons
Bacteriovorax; DGGE analysis; analyse DGGE; bacterial communities; bactéries dégradant les hydrocarbures; communautés bactériennes; hydrocarbon-degrading bacteria; marine protected areas; zones de protection marines