Development and validation of functional CAPS markers for the FAE genes inBrassica junceaand their use in marker-assisted selection.
Low erucic acid is a major breeding target to improve the edible oil quality inBrassica juncea. The single nucleotide polymorphism (SNP) infatty acid elongase 1(FAE1.1andFAE1.2) gene was exploited to expedite the breeding program. The paralogs ofFAE1gene were sequenced from low erucic acid genotype Pusa Mustard 30 and SNPs were identified through homologous alignment with sequence downloaded from NCBI GenBank. Two SNPs inFAE1.1at position 591 and 1265 and one inFAE1.2at 237 were found polymorphic among low and high erucic acid genotypes. These SNPs either create or change the recognition site of restriction enzymes. Transition of a single nucleotide at position 591 and 1265 inFAE1.1, and at position 237 inFAE1.2, leads to a change in the recognition site ofHpy99I,BglIIandMnlIrestriction enzymes, respectively. Two CAPS markers forFAE1.1and one forFAE1.2were developed to differentiate low and high erucic acid genotypes. The efficiency of these CAPS markers was found 100 per cent when validated inBrassica juncea, andB. nigragenotypes and used in back-cross breeding. These CAPS markers will facilitate in marker-assisted selection for improvement of oil quality inBrassica juncea.
Division of Genetics, ICAR-Indian Agricultural Research Institute , New Delhi , India.
Navinder
N
Saini
Division of Genetics, ICAR-Indian Agricultural Research Institute , New Delhi , India.
Naveen
N
Singh
Division of Genetics, ICAR-Indian Agricultural Research Institute , New Delhi , India.
Anil
A
Kumar
Division of Genetics, ICAR-Indian Agricultural Research Institute , New Delhi , India.
Nitika
N
Vihan
Division of Seed Science and Technology, ICAR-IARI , New Delhi , India.
Sangita
S
Yadav
Division of Genetics, ICAR-Indian Agricultural Research Institute , New Delhi , India.
Sujata
S
Vasudev
Division of Genetics, ICAR-Indian Agricultural Research Institute , New Delhi , India.
D K
DK
Yadava
Brassica juncea; CAPS; FAE1; SNP; erucic acid