Quantitative Proteomics Charts the Landscape of Salmonella Carbon Metabolism within Host Epithelial Cells.
We performed a proteomic survey of Salmonella enterica serovar Typhimurium during infection of host epithelial cells. Our data reveal substantial metabolic reshuffling of Salmonella in the host in addition to severe degeneration of bacterial flagella and chemotaxis systems. The large-scale quantitative data allowed us to chart an overview of intracellular Salmonella carbon metabolism. Notably, we found preferential utilization of glycolysis, the pentose phosphate pathway, mixed acid fermentation, and nucleotide metabolism. In contrast, the tricarboxylic acid (TCA) cycle and aerobic and anaerobic respiration pathways were largely repressed. Furthermore, inactivation of glycolysis and purine biosynthesis led to severe growth defects, indicating important roles in intracellular Salmonella replication. In summary, we exploited quantitative proteomics for rational design of follow-up genetic studies and identified pathways important for bacterial fitness within host cells.
Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.
Yanhua
Y
Liu
Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.
Kaiwen
K
Yu
Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.
Fan
F
Zhou
Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.
Tao
T
Ding
Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.
Yufei
Y
Yang
Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.
Mo
M
Hu
Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.
Xiaoyun
0000-0001-7083-5263
X
Liu
D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism*; D006019:Glycolysis; D054884:Host-Pathogen Interactions / Q000235:genetics; D006801:Humans; D040901:Proteomics / Q000379:methods*; D011687:Purines / Q000096:biosynthesis; D019779:Salmonella enterica / Q000378:metabolism* / Q000472:pathogenicity
D001426:Bacterial Proteins; D011687:Purines; D002244:Carbon; C030985:purine
Salmonella metabolism; bacterial infection; mass spectrometry; proteomics