Micro-Topographies Promote Late Chondrogenic Differentiation Markers in the ATDC5 Cell Line.
Chemical and mechanical cues are well-established influencers of in vitro chondrogenic differentiation of ATDC5 cells. Here, we investigate the role of topographical cues in this differentiation process, a study not been explored before. Previously, using a library of surface micro-topographies we found some distinct patterns that induced alkaline phosphatase (ALP) production in human mesenchymal stromal cells. ALP is also a marker for hypertrophy, the end stage of chondrogenic differentiation preceding bone formation. Thus, we hypothesized that these patterns could influence end-stage chondrogenic differentiation of ATDC5 cells. In this study, we randomly selected seven topographies among the ALP influencing hits. Cells grown on these surfaces displayed varying nuclear shape and actin filament structure. When stimulated with insulin-transferrin-selenium (ITS) medium, nodule formation occurred and in some cases showed alignment to the topographical patterns. Gene expression analysis of cells growing on topographical surfaces in the presence of ITS medium revealed a downregulation of early markers and upregulation of late markers of chondrogenic differentiation compared to cells grown on a flat surface. In conclusion, we demonstrated that surface topography in addition to other cues can promote hypertrophic differentiation suitable for bone tissue engineering.
1 Department of Tissue Regeneration, MIRA Institute, University of Twente , Enschede, The Netherlands .
Bach Q
BQ
Le
2 Laboratory for Cell Biology-inspired Tissue Engineering, MERLN Institute, University of Maastricht , Maastricht, The Netherlands .
Aliaksei
A
Vasilevich
2 Laboratory for Cell Biology-inspired Tissue Engineering, MERLN Institute, University of Maastricht , Maastricht, The Netherlands .
Steven
S
Vermeulen
1 Department of Tissue Regeneration, MIRA Institute, University of Twente , Enschede, The Netherlands .
Frits
F
Hulshof
3 Department of Biomaterials Science and Technology, MIRA Institute, University of Twente , Enschede, The Netherlands .
Dimitrios F
DF
Stamatialis
1 Department of Tissue Regeneration, MIRA Institute, University of Twente , Enschede, The Netherlands .|4 Department of Complex Tissue Regeneration, MERLN Institute, University of Maastricht , Maastricht, The Netherlands .
Clemens A
CA
van Blitterswijk
2 Laboratory for Cell Biology-inspired Tissue Engineering, MERLN Institute, University of Maastricht , Maastricht, The Netherlands .
Jan
J
de Boer
D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D000943:Antigens, Differentiation / Q000096:biosynthesis*; D002460:Cell Line; D020219:Chondrogenesis; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D023822:Tissue Engineering; D055114:X-Ray Microtomography
D000943:Antigens, Differentiation; D000469:Alkaline Phosphatase
ATDC5; cell morphology; chondrogenesis; hypertrophy; surface topography