Anti-Neurodegenerative Biflavonoid Glycosides from Impatiens balsamina.
Four biflavonoid glycosides, balsamisides A-D (1-4), and nine known compounds (5-13) were obtained from the white petals of Impatiens balsamina. The 2D structures of the purified phytochemicals were established using conventional NMR techniques in addition to the new long-range HSQMBC NMR experiment. Acid hydrolysis followed by experimental and quantum-mechanics-based ECD data analysis permitted full configurational assignment of the purified metabolites. Compounds 1-13 were assessed for their potential to impede the generation of nitric oxide in lipopolysaccharide-stimulated BV2 cells. They were also investigated for potential neuroprotective activity using C6 cells and cytotoxicity against some human tumor cell lines, but were inactive (IC50> 10 μM) against all the cell lines.
Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University , Suwon 16419, Republic of Korea.
Chung Sub
CS
Kim
Natural Products Research Institute, College of Pharmacy, Seoul National University , Seoul 08826, Republic of Korea.
Munhyung
M
Bae
Department of Chemistry, Yale University , New Haven, Connecticut 06520, United States.|Chemical Biology Institute, Yale University , West Haven, Connecticut 06516, United States.
Joonseok
J
Oh
Gachon Institute of Pharmaceutical Science, Gachon University , Incheon 21936, Republic of Korea.|College of Pharmacy, Gachon University , #191, Hambakmoero, Yeonsu-gu, Incheon 21936, Republic of Korea.
Lalita
L
Subedi
Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University , Suwon 16419, Republic of Korea.
Won Se
WS
Suh
Dong-A ST Research Institute , Kiheung, Yongin 17073, Republic of Korea.
Sang Zin
SZ
Choi
Dong-A ST Research Institute , Kiheung, Yongin 17073, Republic of Korea.
Mi Won
MW
Son
Gachon Institute of Pharmaceutical Science, Gachon University , Incheon 21936, Republic of Korea.|College of Pharmacy, Gachon University , #191, Hambakmoero, Yeonsu-gu, Incheon 21936, Republic of Korea.
Sun Yeou
SY
Kim
Korea Research Institute of Chemical Technology , Daejeon 34114, Republic of Korea.
Sang Un
SU
Choi
Natural Products Research Institute, College of Pharmacy, Seoul National University , Seoul 08826, Republic of Korea.
Dong-Chan
0000-0001-6405-5535
DC
Oh
Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University , Suwon 16419, Republic of Korea.
Kang Ro
0000-0002-3725-5192
KR
Lee
D000465:Algorithms; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D044946:Biflavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans; D029069:Impatiens / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D018696:Neuroprotective Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D009569:Nitric Oxide / Q000096:biosynthesis; D019906:Nuclear Magnetic Resonance, Biomolecular; D056910:Republic of Korea
D000894:Anti-Inflammatory Agents, Non-Steroidal; D044946:Biflavonoids; D006027:Glycosides; D008070:Lipopolysaccharides; D018696:Neuroprotective Agents; D009569:Nitric Oxide