Suprachiasmatic vasopressin to paraventricular oxytocin neurocircuit in the hypothalamus relays light reception to inhibit feeding behavior.
Light synchronizes the body's circadian rhythms by modulating the master clock located in the suprachiasmatic nucleus (SCN) of the hypothalamus. In modern lifestyles that run counter to normal circadian rhythms, the extended and/or irregular light exposure impairs circadian rhythms and, consequently, promotes feeding and metabolic disorders. However, the neuronal pathway through which light is coupled to feeding behavior is less elucidated. The present study employed the light exposure during the dark phase of the day in rats and observed its effect on neuronal activity and feeding behavior. Light exposure acutely suppressed food intake and elevated c-Fos expression in the AVP neurons of SCN and the oxytocin (Oxt) neurons of paraventricular nucleus (PVN) in the hypothalamus. The light-induced suppression of food intake was abolished by blockade of the Oxt receptor in the brain. Retrograde tracer analysis demonstrated the projection of SCN AVP neurons to the PVN. Furthermore, intracerebroventricular injection of AVP suppressed food intake and increased c-Fos in PVN Oxt neurons. Intra-PVN injection of AVP exerted a stronger anorexigenic effect than intracerebroventriclar injection. AVP also induced intracellular Ca2+signaling and increased firing frequency in Oxt neurons in PVN slices. These results reveal the novel neurocircuit from SCN AVP to PVN Oxt that relays light reception to inhibition of feeding behavior. This light-induced neurocircuit may serve as a pathway for forming the circadian feeding rhythm and linking irregular light exposure to arrhythmic feeding and, consequently, obesity and metabolic diseases.
Department of Physiology, Division of Integrative Physiology, Jichi Medical University School of Medicine, Shimotsuke, Tochigi , Japan.
Putra
P
Santoso
Department of Physiology, Division of Integrative Physiology, Jichi Medical University School of Medicine, Shimotsuke, Tochigi , Japan.
Masanori
M
Nakata
Department of Physiology, University of Occupational and Environmental Health , Kitakyushu , Japan.
Yoichi
Y
Ueta
Department of Physiology, Division of Integrative Physiology, Jichi Medical University School of Medicine, Shimotsuke, Tochigi , Japan.
Toshihiko
T
Yada
D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism* / Q000494:pharmacology / Q000502:physiology; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology; D002940:Circadian Rhythm / Q000502:physiology; D005247:Feeding Behavior / Q000187:drug effects / Q000502:physiology*; D007031:Hypothalamus / Q000378:metabolism / Q000502:physiology; D007266:Inhibition, Psychological; D008027:Light; D008297:Male; D009434:Neural Pathways; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D010121:Oxytocin / Q000378:metabolism* / Q000502:physiology; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism* / Q000502:physiology; D016760:Proto-Oncogene Proteins c-fos / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018045:Receptors, Oxytocin; D013493:Suprachiasmatic Nucleus / Q000378:metabolism* / Q000502:physiology
D016760:Proto-Oncogene Proteins c-fos; D018045:Receptors, Oxytocin; D001127:Arginine Vasopressin; D010121:Oxytocin
PVN; circadian rhythm; feeding rhythm; light; metabolic disorders SCN; oxytocin; vasopressin