Different Gene Expression and Activity Pattern of Antioxidant Enzymes in Bladder Cancer.
The aim of this study was to evaluate the possible role in and contribution of antioxidant enzymes to bladder cancer (BC) etiology and recurrence after transurethral resection (TUR). We enrolled 40 patients with BC who underwent TUR and 100 sex- and age-matched healthy controls. The analysis was performed at diagnosis and recurrence, taking into account the time of recurrence. Gene expression of catalase (CAT), glutathione peroxidase 1 (GPX1) and manganese superoxide dismutase (SOD2) was determined in peripheral blood leukocytes. The activity of glutathione peroxidase 3 (GPX3) was examined in plasma, and GPX1 and copper-zinc containing superoxide dismutase 1 (SOD1) in erythrocytes. SOD2 and GPX1 expression and GPX1 and SOD1 activity were significantly higher in patients at diagnosis of BC in comparison to controls. In patients who had recurrence earlier than 1 year from TUR, CAT and SOD2 expression was lower (at diagnosis p=0.024 and p=0.434, at recurrence p=0.022 and p=0.010), while the GPX1 and GPX3 activity was higher (at diagnosis p=0.242 and p=0.394, at recurrence p=0.019 and p=0.025) compared to patients with recurrence after 1 year from TUR. This study revealed that the gene expression and activity of the antioxidant enzymes are elevated in blood of patients with BC, although a low expression of CAT might contribute to the recurrence of BC, in early prognosis.
Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Lodz, Poland edytaw@imp.lodz.pl.
Edyta
E
Wieczorek
First Department of Urology, Medical University of Lodz, Lodz, Poland.
Zbigniew
Z
Jablonowski
Department of Biostatistics and Translational Medicine, Medical University of Lodz, Lodz, Poland.
Bartlomiej
B
Tomasik
Department of Biological and Environmental Monitoring, Nofer Institute of Occupational Medicine, Lodz, Poland.
Jolanta
J
Gromadzinska
Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Lodz, Poland.
Ewa
E
Jablonska
First Department of Urology, Medical University of Lodz, Lodz, Poland.
Tomasz
T
Konecki
Department of Biostatistics and Translational Medicine, Medical University of Lodz, Lodz, Poland.
Wojciech
W
Fendler
First Department of Urology, Medical University of Lodz, Lodz, Poland.
Marek
M
Sosnowski
Department of Biological and Environmental Monitoring, Nofer Institute of Occupational Medicine, Lodz, Poland.
Wojciech
W
Wasowicz
Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Lodz, Poland.
Edyta
E
Reszka
D000368:Aged; D000704:Analysis of Variance; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D004798:Enzymes / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D017063:Outcome Assessment, Health Care; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013053:Spectrophotometry; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D000072105:Superoxide Dismutase-1 / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D001749:Urinary Bladder Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000601:surgery; D000094765:Glutathione Peroxidase GPX1; D000099156:Superoxide Dismutase 2
D000975:Antioxidants; D002374:Catalase; D004798:Enzymes; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D000094765:Glutathione Peroxidase GPX1; D000099156:Superoxide Dismutase 2; C000606290:SOD1 protein, human; C486484:GPX3 protein, human; C000722067:GPX1 protein, human
CAT; Urinary bladder; activity; antioxidant enzymes; bladder cancer recurrence; gene expression