Photoacoustic and Fluorescence Imaging of Cutaneous Squamous Cell Carcinoma in Living Subjects Using a Probe Targeting Integrin αvβ6.
Cutaneous squamous cell carcinoma (cSCC) is the second most common non-melanoma skin cancer worldwide. Today, cSCC is diagnosed by visual inspection followed by invasive skin biopsy. There is a need to develop non-invasive diagnostic tools to achieve early and accurate detection. Photoacoustic imaging (PAI) possesses high ultrasonic resolution and strong optical contrast at new depths (<1-5 cm). Together with exogenous contrast agents, PAI has found promising use in various tumors in living subjects. The expression of integrin αvβ6is significantly up-regulated in cSCC. We fabricated an anti-integrin αvβ6antibody and labeled it with indocyanine green (ICG) to form an ICG-αvβ6antibody. The results showed that the ICG-αvβ6antibody probe could be used to detect cSCC with high specificity (3-fold over the control by PAI) and deep penetration (approximately 1 cm) by PAI. This suggests that the ICG-αvβ6antibody is a promising probe targeting the integrin αvβ6for detection of cSCC tumors by PAI and fluorescence imaging. It may find clinical application in the early diagnosis of cSCC as well as in intraoperative navigation.
Department of Medical Ultrasound, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Chao
C
Zhang
Department of Dermatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Yong
Y
Zhang
Department of Medical Ultrasound, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Kai
K
Hong
Department of Medical Ultrasound, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Shu
S
Zhu
Department of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Jie
J
Wan
D000915:Antibody Affinity; D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000000981:diagnostic imaging / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D018796:Immunoconjugates; D016023:Integrins / Q000235:genetics / Q000378:metabolism*; D057054:Molecular Imaging / Q000379:methods; D061848:Optical Imaging / Q000379:methods; D012878:Skin Neoplasms / Q000000981:diagnostic imaging / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution
D000951:Antigens, Neoplasm; D018796:Immunoconjugates; D016023:Integrins; C073878:integrin alphavbeta6