Hydrogel derived from decellularized porcine adipose tissue as a promising biomaterial for soft tissue augmentation.
Decellularized extracellular matrix (ECM) scaffolds from human adipose tissue, characterized by impressive adipogenic induction ability, are promising for soft tissue augmentation. However, scaffolds from autologous human adipose tissue are limited by the availability of tissue resources and the time necessary for scaffold fabrication. The objective of the current study was to investigate the adipogenic properties of hydrogels of decellularized porcine adipose tissue (HDPA). HDPA induced the adipogenic differentiation of human adipose-derived stem cells (ADSCs) in vitro, with significantly increased expression of adipogenic genes. Subcutaneous injection of HDPA in immunocompetent mice induced host-derived adipogenesis without cell seeding, and adipogenesis was significantly enhanced with ADSCs seeding. The newly formed adipocytes were frequently located on the basal side in the non-seeding group, but this trend was not observed in the ADSCs seeding group. Our results indicated that, similar to human adipose tissue, the ECM scaffold derived from porcine adipose tissue could provide an adipogenic microenvironment for adipose tissue regeneration and is a promising biomaterial for soft tissue augmentation. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1756-1764, 2017.
Department of Breast Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.|Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.
Qiu-Wen
QW
Tan
Department of Breast Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.
Yi
Y
Zhang
Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.
Jing-Cong
JC
Luo
Department of Breast Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.
Di
D
Zhang
Department of Breast Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.
Bin-Jun
BJ
Xiong
Department of Breast Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.
Ji-Qiao
JQ
Yang
Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.
Hui-Qi
HQ
Xie
Department of Breast Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.
Qing
Q
Lv
D017667:Adipocytes / Q000166:cytology*; D050156:Adipogenesis; D000273:Adipose Tissue / Q000737:chemistry* / Q000166:cytology; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000737:chemistry*; D005260:Female; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008297:Male; D008819:Mice, Nude; D013552:Swine; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials; D020100:Hydrogels
ECM scaffold; adipogenesis; adipose-derived stem cell; hydrogel; tissue engineering