Bicarbonate Induced Redox Proteome Changes in Arabidopsis Suspension Cells.
Climate change as a result of increasing atmospheric CO2affects plant growth and productivity. CO2is not only a carbon donor for photosynthesis but also an environmental signal that can perturb cellular redox homeostasis and lead to modifications of redox-sensitive proteins. Although redox regulation of protein functions has emerged as an important mechanism in several biological processes, protein redox modifications and how they function in plant CO2response remain unclear. Here a new iodoTMTRAQ proteomics technology was employed to analyze changes in protein redox modifications inArabidopsis thalianasuspension cells in response to bicarbonate (mimic of elevated CO2) in a time-course study. A total of 47 potential redox-regulated proteins were identified with functions in carbohydrate and energy metabolism, transport, ROS scavenging, cell structure modulation and protein turnover. This inventory of previously unknown redox responsive proteins in Arabidopsis bicarbonate responses lays a foundation for future research toward understanding the molecular mechanisms underlying plant CO2responses.
Plant Molecular and Cellular Biology Program, Department of Biology, Genetics Institute, University of FloridaGainesville, FL, USA; Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Alkali Soil Natural Environmental Science Center, Ministry of Education, Northeast Forestry UniversityHarbin, China.
Zepeng
Z
Yin
Plant Molecular and Cellular Biology Program, Department of Biology, Genetics Institute, University of Florida Gainesville, FL, USA.
Kelly
K
Balmant
Plant Molecular and Cellular Biology Program, Department of Biology, Genetics Institute, University of Florida Gainesville, FL, USA.
Sisi
S
Geng
Plant Molecular and Cellular Biology Program, Department of Biology, Genetics Institute, University of FloridaGainesville, FL, USA; Interdisciplinary Center for Biotechnology Research, University of FloridaGainesville, FL, USA.
Ning
N
Zhu
Plant Molecular and Cellular Biology Program, Department of Biology, Genetics Institute, University of Florida Gainesville, FL, USA.
Tong
T
Zhang
Thermo Fisher Scientific West Palm Beach, FL, USA.
Craig
C
Dufresne
Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Alkali Soil Natural Environmental Science Center, Ministry of Education, Northeast Forestry University Harbin, China.
Shaojun
S
Dai
Plant Molecular and Cellular Biology Program, Department of Biology, Genetics Institute, University of FloridaGainesville, FL, USA; Interdisciplinary Center for Biotechnology Research, University of FloridaGainesville, FL, USA.
Sixue
S
Chen
Arabidopsis thaliana; CO2; bicarbonate; iTRAQ; iodoTMT; oxidative stress; redox proteomics