Sialoglycoprotein isolated from the eggs of Gadus morhua enhances fracture healing in osteoporotic mice.
Osteoporosis is a common disease in the elderly, which is related to fracture healing delay. In this study, the effects of treatment with sialoglycoprotein isolated from the eggs of Gadus morhua (Gm-SGP) on tibial fracture healing in ovariectomized (OVX) osteoporotic female C57BL/6J mice for 56 days post-fracture were investigated. The result showed that Gm-SGP treatment significantly increased serum angiogenic factors and bone formation markers on day 5 and 11 post-fracture when compared with the OVX group. In addition, histological results in the Gm-SGP group showed a stronger endochondral ossification, a stronger bony consolidation and a stronger bony callus remodeling capability on day 11, 24 and 35 post-fracture, respectively, in comparison with the OVX group. Meanwhile, micro-computerized tomography revealed that the Gm-SGP group had stronger bony callus remodeling capability as evidenced by higher BV/TV and Tb.N but lower Tb.Sp and shorter lengths of callus maximum cross section than the OVX group on day 24 post-fracture. Besides, the tibial callus bending stiffness was significantly enhanced in the Gm-SGP group as compared with the OVX group on day 56 post-fracture. Moreover, gene expression suggested that Gm-SGP promoted vascular invasion and endochondral ossification on day 11 post-fracture as well as bone formation on day 11 and 24 post-fracture via up-regulating the expression of angiogenesis factors (including VEGF, PDGF and Ang1), entochondrostosis factors (including Col2a1, Aggrecan, Col10a1 and MMP-13) and osteogenesis markers (including Col1a1, BMP-2 and OCN). This research suggests that Gm-SGP significantly improve fracture healing which is delayed by OVX-induced osteoporosis. The present study may contribute to providing important implications for the utilization of Gm-SGP from fish eggs as a functional food to enhance fracture healing.
College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266003, China. jfwang@ouc.edu.cn xuech@ouc.edu.cn.
Qiping
http://orcid.org/0000-0002-1043-1010
Q
Zhan
School of Basic Medicine, Faculty of Medicine, Guangxi University of Science and Technology, Liuzhou, China.
Xiong
X
Gui
College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266003, China. jfwang@ouc.edu.cn xuech@ouc.edu.cn.
Fei
F
Wang
College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266003, China. jfwang@ouc.edu.cn xuech@ouc.edu.cn.
Peng
P
Yu
College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266003, China. jfwang@ouc.edu.cn xuech@ouc.edu.cn.
Meihui
M
Zhao
College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266003, China. jfwang@ouc.edu.cn xuech@ouc.edu.cn.
Jingfeng
J
Wang
College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266003, China. jfwang@ouc.edu.cn xuech@ouc.edu.cn.
Changhu
C
Xue
D000818:Animals; D004531:Eggs / Q000032:analysis*; D005260:Female; D029941:Fish Proteins / Q000008:administration & dosage* / Q000302:isolation & purification; D017102:Fracture Healing / Q000187:drug effects*; D050723:Fractures, Bone / Q000188:drug therapy* / Q000209:etiology / Q000503:physiopathology; D048389:Gadus morhua; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D010012:Osteogenesis / Q000187:drug effects; D010024:Osteoporosis / Q000150:complications* / Q000503:physiopathology; D012795:Sialoglycoproteins / Q000008:administration & dosage* / Q000302:isolation & purification
D029941:Fish Proteins; D012795:Sialoglycoproteins