A new real-time RT-PCR targeting VP4-VP2 to detect and quantify enterovirus D68 in respiratory samples.
Causing an international outbreak of respiratory disease, Enterovirus D68 quickly entered the closed circle of emerging viral pathogens of public health significance. As rapid and accurate detection of EV-D68 is essential for an efficient clinical management, we designed and validated a new highly efficient one-step quantitative rRT-PCR specific to EV-D68 VP4-VP2 region. With 100% specificity and 95.6% sensitivity to all EV-D68 strains, this new assay can be reliably used to detect and quantify EV-D68 in respiratory samples and represents an interesting additional tool for diagnosis as it targets an original region of the genome.
Faculty of Medicine, University of Geneva, Geneva, Switzerland.|Laboratory of Virology, Division of Infectious Diseases, University of Geneva Hospitals, Geneva, Switzerland.
Léna
L
Royston
Faculty of Medicine, University of Geneva, Geneva, Switzerland.|Laboratory of Virology, Division of Infectious Diseases, University of Geneva Hospitals, Geneva, Switzerland.
Johan
J
Geiser
Centre National de Référence des Enterovirus et Parechovirus, Laboratoire de Virologie, Hospices Civils de Lyon, Lyon, France.
Laurence
L
Josset
Centre National de Référence des Enterovirus et Parechovirus, Laboratoire de Virologie, Hospices Civils de Lyon, Lyon, France.
Isabelle
I
Schuffenecker
Faculty of Medicine, University of Geneva, Geneva, Switzerland.|Laboratory of Virology, Division of Infectious Diseases, University of Geneva Hospitals, Geneva, Switzerland.
Caroline
C
Tapparel
D004196:Disease Outbreaks; D030016:Enterovirus D, Human / Q000235:genetics / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D006801:Humans; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000821:virology*; D012621:Seasons; D012680:Sensitivity and Specificity; D015678:Viral Structural Proteins / Q000235:genetics*
D015678:Viral Structural Proteins
EV-D68; diagnosis; enterovirus; real-time PCR; respiratory viruses