Stabilization Effect of Amino Acid Side Chains in Peptide Assemblies on Graphite Studied by Scanning Tunneling Microscopy.
An analysis is presented of the effects of amino acid side chains on peptide assemblies in ambient conditions on a graphite surface. The molecularly resolved assemblies of binary peptides are examined with scanning tunneling microscopy. A comparative analysis of the assembly structures reveals that the lamellae width has an appreciable dependence on the peptide sequence, which could be considered as a manifestation of a stabilizing effect of side-chain moieties of amino acids with high (phenylalanine) and low (alanine, asparagine, histidine and aspartic acid) propensities for aggregation. These amino acids are representative for the chemical structures involving the side chains of charged (histidine and aspartic acid), aromatic (phenylalanine), hydrophobic (alanine), and hydrophilic (asparagine) amino acids. These results might provide useful insight for understanding the effects of sequence on the assembly of surface-bound peptides.
CAS Key Laboratory of Biological Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience & CAS Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology, No. 11 ZhongGuanCun BeiYiTiao, 100190, Beijing, P.R. China.
Yuanyuan
Y
Guo
CAS Key Laboratory of Biological Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience & CAS Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology, No. 11 ZhongGuanCun BeiYiTiao, 100190, Beijing, P.R. China.
Jingfei
J
Hou
CAS Key Laboratory of Biological Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience & CAS Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology, No. 11 ZhongGuanCun BeiYiTiao, 100190, Beijing, P.R. China.
Xuemei
X
Zhang
CAS Key Laboratory of Biological Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience & CAS Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology, No. 11 ZhongGuanCun BeiYiTiao, 100190, Beijing, P.R. China.
Yanlian
Y
Yang
CAS Key Laboratory of Biological Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience & CAS Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology, No. 11 ZhongGuanCun BeiYiTiao, 100190, Beijing, P.R. China.|University of the Chinese Academy of Sciences, No. 19A Yuquan Road, Shijingshan District, 100049, Beijing, P.R. China.|Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, No. 320 Yue Yang Road, 200031, Shanghai, P.R. China.
Chen
C
Wang
D000596:Amino Acids / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D016252:Microscopy, Scanning Tunneling; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D013499:Surface Properties
D000596:Amino Acids; D010455:Peptides; D006108:Graphite
aggregation propensity; graphite; peptide assembly; scanning tunneling microscopy; sequence effects