Nanoscale mapping of newly-synthesised phospholipid molecules in a biological cell using tip-enhanced Raman spectroscopy.
Nanoscale chemical mapping of newly-synthesised phospholipid molecules inside a mammalian cell is demonstrated using tip-enhanced Raman spectroscopy (TERS) for the first time using mouse pre-adipocyte cells as a model system. Newly-synthesised membrane phospholipid distribution within a pre-adipocyte cell is mapped with <20 nm spatial resolution, overcoming the diffraction limit of confocal Raman spectroscopy via plasmonic enhancement of Raman signals at a TERS tip-apex.
National Physical Laboratory, Hampton Road, Teddington, TW11 0LW, UK. naresh.kumar@npl.co.uk naresh.kumar.gautam@gmail.com.
Naresh
http://orcid.org/0000-0001-8953-5420
N
Kumar
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, OX1 3RE, UK.
Marek M
MM
Drozdz
Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Crawley WA 6009, Australia.
Haibo
H
Jiang
Downing College, University of Cambridge, CB2 1DQ, UK.
Daniela M
DM
Santos
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, OX1 3RE, UK.
David J
DJ
Vaux
D017667:Adipocytes / Q000737:chemistry / Q000648:ultrastructure*; D000818:Animals; D003903:Deuterium; D007425:Intracellular Membranes / Q000737:chemistry / Q000648:ultrastructure*; D051379:Mice; D010713:Phosphatidylcholines / Q000737:chemistry / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D013059:Spectrum Analysis, Raman / Q000379:methods
D010713:Phosphatidylcholines; D010743:Phospholipids; D003903:Deuterium; C010942:1,2-distearoyllecithin