Immobilization of heparin/poly-l-lysine microspheres on medical grade high nitrogen nickel-free austenitic stainless steel surface to improve the biocompatibility and suppress thrombosis.
Thrombosis formation, restenosis, and delayed endothelium regeneration continue to be a challenge for coronary artery stent therapy. To improve the hemocompatibility of cardiovascular implants and to selectively direct vascular cell behavior, a novel heparin/poly-l-lysine microsphere was developed and immobilized on a dopamine-coated surface. We chose medical grade high nitrogen nickel-free austenitic stainless steel as the stent material since it has better biocompatibility. The stability and structural characteristics of the microspheres changed with the heparin: poly-l-lysine concentration ratio. Antithrombin III binding was significantly enhanced. Furthermore, for plasma coagulation tests, the activated partial thromboplastin time and thrombin time were prolonged and depended on the heparinfunction. The modified exhibited excellent stability and anticoagulant activity, and efficiently accelerated endothelialization and anticoagulation. This work has potential application for the design of coronary artery stent surfaces tailored for vascular cell behavior.
Key Laboratory of Biorheological Science and Technology, Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College, Chongqing University, Chongqing, China.
Menghua
M
Li
Chongqing Key Laboratory of Nano/Micro Composite Materials and Devices, School of Metallury and Materials Engineering, Chongqing University of Science and Technology, Chongqing, China; Key Laboratory of Biorheological Science and Technology, Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College, Chongqing University, Chongqing, China.
Haide
H
Wu
Key Laboratory of Biorheological Science and Technology, Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College, Chongqing University, Chongqing, China.
Yi
Y
Wang
Key Laboratory of Biorheological Science and Technology, Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College, Chongqing University, Chongqing, China.
Tieying
T
Yin
Key Laboratory of Biorheological Science and Technology, Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College, Chongqing University, Chongqing, China.
Hans
H
Gregersen
Key Laboratory of Biorheological Science and Technology, Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College, Chongqing University, Chongqing, China.
Xiaojuan
X
Zhang
Chongqing Key Laboratory of Nano/Micro Composite Materials and Devices, School of Metallury and Materials Engineering, Chongqing University of Science and Technology, Chongqing, China.
Xiaoling
X
Liao
Chongqing Key Laboratory of Nano/Micro Composite Materials and Devices, School of Metallury and Materials Engineering, Chongqing University of Science and Technology, Chongqing, China. Electronic address: wanggx@cqu.edu.cn.
Guixue
G
Wang
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001672:Biocompatible Materials / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects / Q000648:ultrastructure; D002452:Cell Count; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D003722:Dental Alloys; D004298:Dopamine / Q000032:analysis; D005340:Fibrinogen / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects; D006493:Heparin / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects; D006801:Humans; D008863:Microspheres; D009532:Nickel / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism; D009584:Nitrogen / Q000494:pharmacology*; D010314:Partial Thromboplastin Time; D010316:Particle Size; D010973:Platelet Adhesiveness / Q000187:drug effects; D011107:Polylysine / Q000494:pharmacology*; D011517:Prothrombin Time; D011817:Rabbits; D013193:Stainless Steel / Q000494:pharmacology*; D055672:Static Electricity; D013499:Surface Properties; D013927:Thrombosis / Q000473:pathology / Q000517:prevention & control*
D000975:Antioxidants; D001672:Biocompatible Materials; D003722:Dental Alloys; C093826:Austenite; D013193:Stainless Steel; D011107:Polylysine; D009569:Nitric Oxide; D009532:Nickel; D005340:Fibrinogen; D006493:Heparin; D009584:Nitrogen; D004298:Dopamine
Blood compatibility; Coronary stent; Heparin; High nitrogen nickel-free austenitic stainless steel; Microsphere