Recombinant Phage Elicits Protective Immune Response against Systemic S. globosa Infection in Mouse Model.
Sporothrix globosa is a type of fungus that typically infects immunocompromised patients. Its prevention continues to pose a challenge. A 70-KDa glycoprotein (Gp70) of Sporothrix has been previously reported to protect host against infection from this fungus. Here, we displayed an epitope peptide (kpvqhalltplgldr) of Gp70 on the major coat protein (pIII), and investigated its efficiency as a vaccine for preventing S. globosa infection. The recombinant phage and the heat-killed S. globosa were used to immunize mice separately. In this study, we evaluated the humoral and cellular immune responses in the mice and demonstrated that recombinant phage could induce mice to produce a stronger immune response and generate antibodies to inhibit S. globosa infection. Furthermore, immunization with recombinant phage could increase the survival rate of S. globosa infection in mice. All these results together indicated that recombinant phages displaying kpvqhalltplgldr are a potential vaccine candidate against S. globosa infection.
Dermatology Department, China-Japan Union Hospital of Jilin University, 126Xiantai Street, Changchun, Jilin Province 130033, People's Republic of China.
Feng
F
Chen
Dermatology Department, China-Japan Union Hospital of Jilin University, 126Xiantai Street, Changchun, Jilin Province 130033, People's Republic of China.
Rihua
R
Jiang
Jilin Provincial Key Laboratory on Molecular and Chemical Genetic, the Second Hospital of Jilin University, 218 Ziqiang Street, Changchun, Jilin Province 130041, People's Republic of China.
Yicun
Y
Wang
Dermatology Department, China-Japan Union Hospital of Jilin University, 126Xiantai Street, Changchun, Jilin Province 130033, People's Republic of China.
Mingji
M
Zhu
Department of Hang Surgery, Second Hospital of Qinhuangdao, Hebei 066600, People's Republic of China.
Xu
X
Zhang
Institute of Cytology and Genetics, School of Life Sciences, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province 130024, People's Republic of China.
Shuai
S
Dong
Institute of Cytology and Genetics, School of Life Sciences, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province 130024, People's Republic of China.
Hongxi
H
Shi
Institute of Cytology and Genetics, School of Life Sciences, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province 130024, People's Republic of China.
Li
L
Wang
D000595:Amino Acid Sequence; D000818:Animals; D000908:Antibodies, Fungal / Q000627:therapeutic use; D001435:Bacteriophages / Q000276:immunology*; D000939:Epitopes; D006023:Glycoproteins / Q000276:immunology*; D007111:Immunity, Cellular; D007114:Immunization / Q000379:methods; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins; D013175:Sporothrix / Q000276:immunology*; D013174:Sporotrichosis / Q000628:therapy*; D014612:Vaccines
D000908:Antibodies, Fungal; D000939:Epitopes; D006023:Glycoproteins; D011994:Recombinant Proteins; D014612:Vaccines