Beta-hydroxybutyrate and pyroglutamate can be included in a rapid GC-MS screening method for differential diagnosis of metabolic acidosis.
A rapid gas chromatographic mass spectrometric method for measuring anions associated with acute anion gap metabolic acidosis is described. The method is an extension of a previous method. The method quantifies glycolic acid, beta-hydroxybutyric acid with good linearity and pyroglutamic acid with a reproducible curvature relation between 1 and 20 mmol/L and can help the clinician distinguish effectively between ethylene glycol poisoning, alcoholic and diabetic ketoacidosis and cysteine deficiency so early that it will have clinical consequences.
a Department of Pharmacology , Oslo University Hospital , Oslo , Norway.
Siri
S
Føreid
a Department of Pharmacology , Oslo University Hospital , Oslo , Norway.
Gaut
G
Gadeholt
D020155:3-Hydroxybutyric Acid / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D002138:Calibration; D003545:Cysteine / Q000172:deficiency; D016883:Diabetic Ketoacidosis / Q000097:blood / Q000175:diagnosis*; D003937:Diagnosis, Differential; D019855:Ethylene Glycol / Q000506:poisoning; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006016:Glycolates / Q000097:blood*; D006801:Humans; D007662:Ketosis / Q000097:blood / Q000175:diagnosis*; D011761:Pyrrolidonecarboxylic Acid / Q000097:blood*; D015203:Reproducibility of Results
D015415:Biomarkers; D006016:Glycolates; C031149:glycolic acid; D019855:Ethylene Glycol; D003545:Cysteine; D011761:Pyrrolidonecarboxylic Acid; D020155:3-Hydroxybutyric Acid
Analytical techniques; clinical pharmacology; clinical toxicology; gas chromatography; mass spectrometry