Method comparison for analyzing wound healing rates.
Wound healing scratch assay is a frequently used method to characterize cell migration, which is an important biological process in the course of development, tissue repair, and immune response for example. The measurement of wound healing rate, however, varies among different studies. Here we summarized these measurements into three types: (I) direct rate average; (II) regression rate average; and (III) average distance regression rate. Using Chinese hamster ovary (CHO) cells as a model, we compared the three types of analyses on quantifying the wound closing rate, and discovered that type I & III measurements are more resistant to outliers, and type II analysis is more sensitive to outliers. We hope this study can help researchers to better use this simple yet effective assay.
a Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC V5A 1S6, Canada.
Prabhpreet K
PK
Dhillon
b Department of Chemistry, Simon Fraser University, Burnaby, BC V5A 1S6, Canada.
Xinyin
X
Li
b Department of Chemistry, Simon Fraser University, Burnaby, BC V5A 1S6, Canada.
Jurgen T
JT
Sanes
b Department of Chemistry, Simon Fraser University, Burnaby, BC V5A 1S6, Canada.
Oluwafemi S
OS
Akintola
a Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC V5A 1S6, Canada.|b Department of Chemistry, Simon Fraser University, Burnaby, BC V5A 1S6, Canada.|c Centre for Cell Biology, Development, and Disease, Simon Fraser University, BC V5A 1S6, Canada.
Bingyun
B
Sun
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D016466:CHO Cells; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation; D006224:Cricetinae; D003412:Cricetulus; D014945:Wound Healing / Q000502:physiology*
Chinese hamster ovary cells; N-acetylglucosamine (GlcNAc); cell migration rate; cellules d’ovaires de hamster chinois; taux de migration cellulaire; test de cicatrisation in vitro; wound healing scratch assay