Molecular identification and expression analysis of TLR5M and TLR5S from orange-spotted grouper (Epinepheluscoioides).
Toll-like receptor 5 (TLR5) is an important receptor that interacts with bacterial flagellin and regulates host immune response in mammal. Recent studies demonstrate that piscine contains two types of TLR5, namely membrane form of TLR5 (TLR5M) and soluble form of TLR5 (TLR5S), and both of which perform crucial role in flagellin response. In the present study, a TLR5M and a TLR5S sequence was cloned from orange-spotted grouper (Epinepheluscoioides), and their ORFs are respectively 2466 bp (821 aas) and 1935 bp (644 aas). EcTLR5M has the typical TLR structure of a LRR domain, a transmembrane region and a TIR domain, while EcTLR5S only contains a LRR domain like other species' TLR5S. Both molecules have 23 LRR motifs, a LRR-NT and a LRR-CT in the LRR domain, similar to those of other species. Phylogenetic and sequence alignment indicated that both EcTLR5s respectively displayed closer relationship and higher sequence identity with those in other fish species. In healthy grouper, EcTLR5M was highly expressed in the skin, head kidney and spleen, while EcTLR5S was mainly detected in the liver. Ciliate Cryptocaryon irritans infection could significantly up-regulate the expression level of EcTLR5s in the gill and spleen from day 1 to day 3, and higher expression fold change was observed in the spleen. Taken together, the present studies contributed to understanding the function of piscine TLR5M/S and clarify their possible role in fish immune response against ciliate infection.
Guangzhou Airport Extry-Exit Inspection and Quarantine Bureau, Guangzhou 510470, Guangdong Province, PR China. Electronic address: bjslinyi@qq.com.
Jian-Shan
JS
Bai
College of Marine Sciences, South China Agricultural University, Guangzhou 510642, Guangdong Province, PR China.
Yan-Wei
YW
Li
Guangzhou Airport Extry-Exit Inspection and Quarantine Bureau, Guangzhou 510470, Guangdong Province, PR China.
Yan
Y
Deng
Guangzhou Airport Extry-Exit Inspection and Quarantine Bureau, Guangzhou 510470, Guangdong Province, PR China.
Yan-Qiong
YQ
Huang
Guangzhou Airport Extry-Exit Inspection and Quarantine Bureau, Guangzhou 510470, Guangdong Province, PR China.
Shu-Hua
SH
He
Guangzhou Airport Extry-Exit Inspection and Quarantine Bureau, Guangzhou 510470, Guangdong Province, PR China.
Jin
J
Dai
Guangzhou Airport Extry-Exit Inspection and Quarantine Bureau, Guangzhou 510470, Guangdong Province, PR China.
Shang-Zhi
SZ
Zhao
College of Marine Sciences, South China Agricultural University, Guangzhou 510642, Guangdong Province, PR China.
Xue-Ming
XM
Dan
College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong Province, PR China.
Xiao-Chun
XC
Luo
D000818:Animals; D001492:Bass / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D016798:Ciliophora / Q000276:immunology; D016770:Ciliophora Infections / Q000276:immunology / Q000469:parasitology / Q000662:veterinary; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D020224:Expressed Sequence Tags; D005393:Fish Diseases / Q000276:immunology / Q000469:parasitology; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D007113:Immunity, Innate / Q000235:genetics; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA / Q000662:veterinary; D051198:Toll-Like Receptor 5 / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary; D029941:Fish Proteins; D012333:RNA, Messenger; D051198:Toll-Like Receptor 5
Cryptocaryon irritans; Innate immune response; TLR5M; TLR5S