mecA-related structure in methicillin-resistant coagulase-negative staphylococci from street food in Taiwan.
Antibiotic-resistant patterns, a mecA homologue complex, and staphylococcal cassette chromosome mec (SCCmec) were analysed in samples of ready-to-eat (RTE) street food in Taiwan. RTE food samples (270) were collected in three densely populated Taiwanese cities between June and November 2014. Among 14 strains being identified as methicillin-resistant coagulase-negative staphylococci (MRCoNS), genetic diversities was determined by PFGE analysis. SCCmec types IV, V, VIII and TXG-24 were detected in 9, and mecASs(a mecA homologue) detected in 8. The mecASsgene complex from S. sciuri subsp. sciuri TXG-24 was found to be closely related to those found in both S. sciuri subsp. sciuri (ATCC29062) and S. sciuri subsp. rodentium (ATCC700061). SCCmecTXG24carries a class A mec complex, a ccrA5B3-like gene complex, a heavy metal gene complex, and an IS1216 mobile element carrying tet(S). Matching identity to ccrA5 was 84.5% for ccrA in S. pseudintermedius KM241. Matching identify to ccrB3 was 92.1% for ccrB in S. pseudintermedius AI16. Similar ccrA and SCCmec boundary sequences suggest that SCCmec is easily transmitted to coagulase-negative staphylococci (CoNS). Based on MRCoNS strains identified in this research, Taiwanese RTE food products likely carry multiple antibiotic resistance genes that can be transmitted to hospitals and other clinical settings.
Department of Medical Laboratory Science and Biotechnology, College of Health Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan.
Tsung-Ying
TY
Yang
Division of Endocrinology and Metabolism, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.
Wei-Wen
WW
Hung
Department of Culinary Art, I-Shou University, Kaohsiung, Taiwan.
Lin
L
Lin
Department of Microbiology and Immunology, Kaohsiung Medical University, Kaohsiung, Taiwan.
Wei-Chun
WC
Hung
Department of Medical Laboratory Science and Biotechnology, College of Health Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan.|Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung, Taiwan.
Sung-Pin
SP
Tseng
D001426:Bacterial Proteins / Q000235:genetics; D002876:Chromosomes, Bacterial; D003030:Coagulase / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005504:Food Analysis; D005516:Food Microbiology; D014644:Genetic Variation / Q000235:genetics; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D046915:Penicillin-Binding Proteins / Q000235:genetics; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000235:genetics / Q000382:microbiology; D013210:Staphylococcus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D013624:Taiwan
D001426:Bacterial Proteins; D003030:Coagulase; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus