Genome-wide analysis of auxin transport genes identifies the hormone responsive patterns associated with leafy head formation in Chinese cabbage.
Auxin resistant 1/like aux1 (AUX/LAX), pin-formed (PIN) and ATP binding cassette subfamily B (ABCB/MDR/PGP) are three families of auxin transport genes. The development-related functions of the influx and efflux carriers have been well studied and characterized in model plants. However, there is scant information regarding the functions of auxin genes in Chinese cabbage and the responses of exogenous polar auxin transport inhibitors (PATIs). We conducted a whole-genome annotation and a bioinformatics analysis of BrAUX/LAX, BrPIN, and BrPGP genes in Chinese cabbage. By analyzing the expression patterns at several developmental stages in the formation of heading leaves, we found that most auxin-associate genes were expressed throughout the entire process of leafy head formation, suggesting that these genes played important roles in the development of heads. UPLC was used to detect the distinct and uneven distribution of auxin in various segments of the leafy head and in response to PATI treatment, indicated that the formation of the leafy head depends on polar auxin transport and the uneven distribution of auxin in leaves. This study provides new insight into auxin polar transporters and the possible roles of the BrLAX, BrPIN and BrPGP genes in leafy head formation in Chinese cabbage.
State Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Biology and Germplasm Enhancement of Horticulture Crops in East China, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, PR China.
Li-Wei
LW
Gao
State Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Biology and Germplasm Enhancement of Horticulture Crops in East China, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, PR China.
Shan-Wu
SW
Lyu
Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing, PR China.
Jun
J
Tang
State Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Biology and Germplasm Enhancement of Horticulture Crops in East China, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, PR China.
Dao-Yun
DY
Zhou
Wageningen UR Plant Breeding, Wageningen University and Research Centre, Wageningen, the Netherlands.
Guusje
G
Bonnema
State Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Biology and Germplasm Enhancement of Horticulture Crops in East China, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, PR China.
Dong
D
Xiao
State Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Biology and Germplasm Enhancement of Horticulture Crops in East China, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, PR China.
Xi-Lin
XL
Hou
State Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Biology and Germplasm Enhancement of Horticulture Crops in East China, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, PR China.
Chang-Wei
CW
Zhang
D001692:Biological Transport / Q000235:genetics; D001937:Brassica / Q000235:genetics*; D032461:Chromosomes, Plant / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D007210:Indoleacetic Acids / Q000378:metabolism*; D059372:Nucleotide Motifs / Q000235:genetics; D009928:Organ Specificity / Q000235:genetics; D010797:Phthalimides / Q000494:pharmacology; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D014283:Triiodobenzoic Acids / Q000494:pharmacology
D007210:Indoleacetic Acids; D010797:Phthalimides; D010937:Plant Growth Regulators; D010940:Plant Proteins; D014283:Triiodobenzoic Acids; C002687:alpha-naphthylphthalamic acid; C052968:2,3,5-triiodobenzoic acid