Nano-Au/cMWCNT ModifiedspeBGene Specific Amperometric Sensor for Rapidly DetectingStreptococcus pyogenescausing Rheumatic Heart Disease.
A specific 5' NH2labeled DNA probe ofspeBgene was immobilized onto the gold nanoparticles/carboxylated multi walled carbon nanotubes (Nano-Au/cMWCNT) screen printed electrode using EDC/NHS cross linking chemistry. This was followed by hybridization with 0.5-50 ng/6 µl of single stranded genomic DNAStreptococcus pyogenesinfected patient throat swab samples. Electrochemical amperometric assay was deciphered by using cyclic voltammetry (CV) with methylene blue a redox indicator. The sensor had a sensitivity of 104.7 µA cm-2ng-1using CV with a R2of 0.907 and 0.01 ng/6 µl as the limit of detection (LOD). The modified electrode surface morphology was characterized using scanning electron microscopy. The stability of the electrode was seen at 4 °C for 180 days having 6% loss in the initial current. The sensor isspeBgene specific and can detect the pathogen within 30 min.
Shoolini University of Biotechnology and Management Sciences, Solan, 173212 India.|CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi, 110007 India.
Ankur
A
Kaushal
CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi, 110007 India.
Swati
S
Singh
CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi, 110007 India.
Ashok
A
Kumar
Shoolini University of Biotechnology and Management Sciences, Solan, 173212 India.
Dinesh
D
Kumar
Nano-Au/cMWCNT; Rheumatic heart disease; Streptococcus pyogenes; speB sensor