DNA barcodes for dragonflies and damselflies (Odonata) of Mindanao, Philippines.
Reliable species identification provides a sounder basis for use of species in the order Odonata as biological indicators and for their conservation, an urgent concern as many species are threatened with imminent extinction. We generated 134 COI barcodes from 36 morphologically identified species of Odonata collected from Mindanao Island, representing 10 families and 19 genera. Intraspecific sequence divergences ranged from 0 to 6.7% with four species showing more than 2%, while interspecific sequence divergences ranged from 0.5 to 23.3% with seven species showing less than 2%. Consequently, no distinct gap was observed between intraspecific and interspecific DNA barcode divergences. The numerous islands of the Philippine archipelago may have facilitated rapid speciation in the Odonata and resulted in low interspecific sequence divergences among closely related groups of species. This study contributes DNA barcodes for 36 morphologically identified species of Odonata reported from Mindanao including 31 species with no previous DNA barcode records.
a Department of Biological Sciences , College of Science and Mathematics, Mindanao State University-Iligan Institute of Technology , Iligan City , Philippines.
Princess Angelie S
PAS
Casas
b State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences , Beijing , China.
Kong-Wah
KW
Sing
c Department of Applied Sciences, Faculty of Science and Technology , Nilai University, Persiaran Universiti, Putra Nilai , Nilai , Negeri Sembilan , Malaysia.
Ping-Shin
PS
Lee
a Department of Biological Sciences , College of Science and Mathematics, Mindanao State University-Iligan Institute of Technology , Iligan City , Philippines.
Olga M
OM
Nuñeza
d D3C Gahol Apartment, Lopez Jaena St. , Davao City , Philippines.
Reagan Joseph T
RJT
Villanueva
e International College Beijing, China Agricultural University , Beijing , P. R. China.
John-James
JJ
Wilson
D000818:Animals; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D019143:Evolution, Molecular; D049810:Genetic Speciation; D063191:Odonata / Q000145:classification* / Q000235:genetics; D010679:Philippines; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000379:methods*; D013045:Species Specificity
COI; DNA barcoding; Odonata; molecular taxonomy