Behavior of Gingival Fibroblasts on Titanium Implant Surfaces in Combination with either Injectable-PRF or PRP.
Various strategies have been employed to speed tissue regeneration using bioactive molecules. Interestingly, platelet concentrates derived from a patient's own blood have been utilized as a regenerative strategy in recent years. In the present study, a novel liquid platelet formulation prepared without the use of anti-coagulants (injectable-platelet-rich fibrin, i-PRF) was compared to standard platelet-rich plasma (PRP) with gingival fibroblasts cultured on smooth and roughened titanium implant surfaces. Standard PRP and i-PRF (centrifuged at 700 rpm (60×g) for 3 min) were compared by assays for fibroblast biocompatibility, migration, adhesion, proliferation, as well as expression of platelet-derived growth factor (PDGF), transforming growth factor-β (TGF-β), collagen1 (COL1) and fibronectin (FN). The results demonstrate that i-PRF induced significantly higher cell migration, as well as higher messenger RNA (mRNA) levels of PDGF, TGF-β, collagen1 and fibronectin when compared to PRP. Furthermore, collagen1 synthesis was highest in the i-PRF group. These findings demonstrate that liquid platelet concentrates can be formulated without the use of anticoagulants and present much translational potential for future research. Future animal and clinical trials are now necessary to further investigate the potential of utilizing i-PRF for soft tissue regenerative protocols in combination with various biomaterials.
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China. wangxuzhu@whu.edu.cn.|Department of Oral Implantology, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China. wangxuzhu@whu.edu.cn.
Xuzhu
X
Wang
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China. zyf@whu.edu.cn.|Department of Oral Implantology, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China. zyf@whu.edu.cn.
Yufeng
Y
Zhang
Pain Clinic, 06000 Nice, France. joseph@a-prf.com.
Joseph
J
Choukroun
FORM, Frankfurt Oral Regenerative Medicine, Clinic for Maxillofacial and Plastic Surgery, Johann Wolfgang Goethe University, 60596 Frankfurt Am Main, Germany. s.ghanaati@med.uni-frankfurt.de.
Shahram
S
Ghanaati
Department of Periodontology, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, FL 33328, USA. rmiron@nova.edu.|Cell Therapy Institute, Collaborative Centre for Research, Nova Southeastern University, Fort Lauderdale, FL 33328, USA. rmiron@nova.edu.|Department of Periodontics and Oral Surgery, University of Ann Arbor, Ann Arbor, MI 48109, USA. rmiron@nova.edu.
Richard J
RJ
Miron
D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D024042:Collagen Type I / Q000378:metabolism; D015921:Dental Implants; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism; D005337:Fibrin / Q000378:metabolism; D005347:Fibroblasts / Q000502:physiology*; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D053657:Platelet-Rich Plasma; D012038:Regeneration / Q000235:genetics; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry; D014945:Wound Healing
D024042:Collagen Type I; D015921:Dental Implants; D016326:Extracellular Matrix Proteins; D005337:Fibrin; D014025:Titanium
blood; fibrin; fibroblasts; platelet-rich fibrin; platelets; regeneration; wound healing