Characterization of Stilbene Synthase Genes in Mulberry (Morus atropurpurea) and Metabolic Engineering for the Production of Resveratrol in Escherichia coli.
Stilbenes have been recognized for their beneficial physiological effects on human health. Stilbene synthase (STS) is the key enzyme of resveratrol biosynthesis and has been studied in numerous plants. Here, four MaSTS genes were isolated and identified in mulberry (Morus atropurpurea Roxb.). The expression levels of MaSTS genes and the accumulation of trans-resveratrol, trans-oxyresveratrol, and trans-mulberroside A were investigated in different plant organs. A novel coexpression system that harbored 4-coumarate:CoA ligase gene (Ma4CL) and MaSTS was established. Stress tests suggested that MaSTS genes participate in responses to salicylic acid, abscisic acid, wounding, and NaCl stresses. Additionally, overexpressed MaSTS in transgenic tobacco elevated the trans-resveratrol level and increased tolerance to drought and salinity stresses. These results revealed the major MaSTS gene, and we evaluated its function in mulberry, laying the foundation for future research on stilbene metabolic pathways in mulberry.
College of Biotechnology, Southwest University , No. 2 Tiansheng Road, BeiBei District, Chongqing 400716, China.
Chuanhong
http://orcid.org/0000-0001-8446-9268
C
Wang
College of Biotechnology, Southwest University , No. 2 Tiansheng Road, BeiBei District, Chongqing 400716, China.
Shuang
S
Zhi
College of Biotechnology, Southwest University , No. 2 Tiansheng Road, BeiBei District, Chongqing 400716, China.
Changying
C
Liu
College of Biotechnology, Southwest University , No. 2 Tiansheng Road, BeiBei District, Chongqing 400716, China.
Fengxiang
F
Xu
College of Biotechnology, Southwest University , No. 2 Tiansheng Road, BeiBei District, Chongqing 400716, China.
Aichun
A
Zhao
College of Biotechnology, Southwest University , No. 2 Tiansheng Road, BeiBei District, Chongqing 400716, China.
Xiling
X
Wang
College of Biotechnology, Southwest University , No. 2 Tiansheng Road, BeiBei District, Chongqing 400716, China.
Yanhong
Y
Ren
The Sericultural and Apicultural Research Institute, Yunnan Academy of Agricultural Sciences , Mengzi, Yunnan 661100, China.
Zhengang
Z
Li
College of Biotechnology, Southwest University , No. 2 Tiansheng Road, BeiBei District, Chongqing 400716, China.
Maode
M
Yu
D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D053898:Biosynthetic Pathways; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D060847:Metabolic Engineering; D031625:Morus / Q000201:enzymology* / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism
D010940:Plant Proteins; D013267:Stilbenes; D000217:Acyltransferases; C022674:stilbene synthase; D000077185:Resveratrol
coexpression; mulberry; resveratrol; stilbene synthase; stilbenes