Graphene quantum dot modified screen printed immunosensor for the determination of parathion.
The widespread use of pesticides has immense effect on increased crop productions. However, they are also responsible for posing detrimental health hazards and/or for contaminating the environment with chemical residues. A routine and an on-field detection of pesticide residues in different food, water, and soil samples has become a need of the hour for which biosensors can offer a viable alternative. The present work reports a functionalized graphene quantum dot (GQD) based screen printed electrochemical immunosensor for the detection of parathion. The application of GQDs has permitted the realization of a sensitive, robust, and reproducible sensor unlike those carried out earlier for the similar purposes. This immunosensor exhibited a dynamic linear response for parathion within the range of 0.01-106ng/L with a very low detection limit of 46 pg/L. According to the analysis of potential interferences, the proposed sensor was specifically detecting parathion even in the presence of its metabolite, paraoxon. The investigations of the proposed sensing approach with respect to stability, response reproducibility, and regeneration have fully supported its potential practical applicability.
Nanoscience and Nanotechnology Lab, Central Scientific Instruments Organisation (CSIR-CSIO), Sector 30 C, Chandigarh 160030, India; Academy of Scientific and Innovative Research (CSIR-CSIO), Sector 30 C, Chandigarh 160030, India.
Jyotsana
J
Mehta
Nanoscience and Nanotechnology Lab, Central Scientific Instruments Organisation (CSIR-CSIO), Sector 30 C, Chandigarh 160030, India; Academy of Scientific and Innovative Research (CSIR-CSIO), Sector 30 C, Chandigarh 160030, India.
Neha
N
Bhardwaj
Nanoscience and Nanotechnology Lab, Central Scientific Instruments Organisation (CSIR-CSIO), Sector 30 C, Chandigarh 160030, India; Academy of Scientific and Innovative Research (CSIR-CSIO), Sector 30 C, Chandigarh 160030, India.
Sanjeev K
SK
Bhardwaj
BioNano Laboratory, School of Engineering, University of Guelph, Guelph, ON, N1G 2W1, Canada.
Satish K
SK
Tuteja
Department of Chemical Engineering, Indian Institute of Technology, Delhi 110016, India.
Priya
P
Vinayak
DAV University, Jalandhar, Punjab, India.
A K
AK
Paul
Department of Civil and Environmental Engineering, Hanyang University, 222 Wangsimni-Ro, Seoul 04763, Republic of Korea. Electronic address: kkim61@hanyang.ac.kr.
Ki-Hyun
KH
Kim
Nanoscience and Nanotechnology Lab, Central Scientific Instruments Organisation (CSIR-CSIO), Sector 30 C, Chandigarh 160030, India; Academy of Scientific and Innovative Research (CSIR-CSIO), Sector 30 C, Chandigarh 160030, India. Electronic address: dr.akashdeep@csio.res.in.
Akash
A
Deep
D055910:Antibodies, Immobilized / Q000737:chemistry*; D015374:Biosensing Techniques; D055664:Electrochemical Techniques / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007306:Insecticides / Q000032:analysis*; D057230:Limit of Detection; D010278:Parathion / Q000032:analysis*; D011327:Printing / Q000295:instrumentation / Q000379:methods*; D045663:Quantum Dots
D055910:Antibodies, Immobilized; D007306:Insecticides; D010278:Parathion; D006108:Graphite
Electrochemical impedance spectroscopy; Graphene quantum dots; Immunosensor; Parathion; Screen-printed electrode