DNA Cytometry for Differentiation Between Low- and Medium-grade Dysplasia in Intraductal Papillary Mucinous Neoplasms.
The indication for resection of cystic pancreatic lesions is usually performed by sectional imaging criteria, such as the Sendai criteria. The aim of this study was to analyze a possible correlation between DNA cytometry and Sendai criteria for the differentiation between low-grade intraductal papillary mucinous neoplasms (IPMN-A) and medium-grade dysplasia (IPMN-B).



Histopathological analysis, DNA index and preoperative Sendai criteria were determined in 16 patients who underwent pancreatic resection for IPMN.



All patients with IPMN-B showed aneuploid histograms with DNA indices ≥1.3, whereas three out of four patients with IPMN-A had diploid DNA indices ≤1.3. All 11 patients with one or more high-risk stigmata and aneuploid histograms had IPMN-Bs, whereas both patients who were Sendai-negative and diploid in the DNA analysis had an IPMN-A.



DNA index may be an important diagnostic tool for the differentiation of different IPMN types beyond the traditional Sendai criteria.
Department of Surgery, Charite University of Medicine, Berlin, Germany fritz.klein@charite.de.
Fritz
F
Klein
Department of Diagnostic and Interventional Radiology, Charite University of Medicine, Berlin, Germany.
Timm
T
Denecke
Department of Surgery, Charite University of Medicine, Berlin, Germany.
Wladimir
W
Faber
Department of Gastroenterology, Infectious Diseases and Rheumatology, Campus Mitte, Charite University of Medicine, Berlin, Germany.
Christian
C
Jürgensen
Department of Surgery, Charite University of Medicine, Berlin, Germany.
Anja
A
Schirmeier
Department of Surgery, Charite University of Medicine, Berlin, Germany.
Hussein
H
Al-Abadi
Department of Diagnostic and Interventional Radiology, Charite University of Medicine, Berlin, Germany.
Thula C
TC
Walter
Department of Surgery, Charite University of Medicine, Berlin, Germany.
Nadine
N
Al-Abadi
Department of Surgery, Charite University of Medicine, Berlin, Germany.
Thomas
T
Malinka
Department of Surgery, Charite University of Medicine, Berlin, Germany.
Johann
J
Pratschke
Department of Surgery, Charite University of Medicine, Berlin, Germany.
Marcus
M
Bahra
D002288:Adenocarcinoma, Mucinous / Q000000981:diagnostic imaging / Q000235:genetics* / Q000601:surgery; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000782:Aneuploidy; D021441:Carcinoma, Pancreatic Ductal / Q000000981:diagnostic imaging / Q000235:genetics* / Q000601:surgery; D002291:Carcinoma, Papillary / Q000000981:diagnostic imaging / Q000235:genetics* / Q000601:surgery; D004273:DNA, Neoplasm / Q000235:genetics*; D003937:Diagnosis, Differential; D004171:Diploidy; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D010179:Pancreas / Q000000981:diagnostic imaging / Q000378:metabolism / Q000601:surgery; D010180:Pancreatectomy / Q000379:methods; D010190:Pancreatic Neoplasms / Q000000981:diagnostic imaging / Q000235:genetics* / Q000601:surgery; D012189:Retrospective Studies
D004273:DNA, Neoplasm
DNA index; IPMN; Sendai criteria; cystic neoplasia; pancreas