Phylogenetic analysis ofEscherichia coliisolated from broilers with colibacillosis based ongyrAgene sequences.
Escherichia coliisolates from chickens with colibacillosis were assigned to phylogenetic groups based on multiplex polymerase chain reaction (PCR) and antibacterial resistance ofE. colibelonging to these groups was examined. Furthermore, thegyrA gene of isolates was sequenced and a phylogenetic tree was generated. A total of 84E. coliisolates were grouped using multiplex PCR of TSPE4.C2,chuA,yjaA, andgadA molecular markers. Four phylogenetic groups were identified with strains divided as follows: 16 in group A (19.05%), 17 in group B1 (20.24%), 23 in group B2 (27.38%), and 28 in group D (33.33%).Escherichia coliisolates belonging to phylogenetic groups B2 and D were resistant to Soltrim and Flumequine unlike the majority ofE. coliisolates that belonged to groups A and B1, and which were susceptible to these antibiotics. The phylogenetic results based ongyrAgene sequences from multiplex PCR revealed thatE. coliphylogenetic grouping was in accordance with the clusters obtained in the phylogenetic tree. In conclusion, the comparative sequence analysis ofgyrAsequences provides a firm framework for an accurate classification ofE. coliand related taxa and may constitute a pertinent phylogenetic marker forE. coli.
Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, West Azerbaijan, Iran (Shamsi, Mardani); Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, West Azerbaijan, Iran (Ownagh).
Hamid
H
Shamsi
Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, West Azerbaijan, Iran (Shamsi, Mardani); Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, West Azerbaijan, Iran (Ownagh).
Karim
K
Mardani
Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, West Azerbaijan, Iran (Shamsi, Mardani); Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, West Azerbaijan, Iran (Ownagh).
Abdolghaffar
A
Ownagh
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002645:Chickens; D027081:DNA Gyrase / Q000235:genetics*; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D010802:Phylogeny; D011201:Poultry Diseases / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D027081:DNA Gyrase