Controlled release of an endostatin peptide using chitosan nanoparticles.
Whereas several anticancer peptides are in different stages of clinical development, their administration is limited by the fast elimination from the systemic circulation. Peptide loading on nano-carriers can pave the way for their future application. We have recently indicated that a disulfide loop rather than a Zn-binding loop improves the anti-angiogenic and antitumor activities of the N-terminal fragment of endostatin. In this study, chitosan nanoparticles (CS NPs) are used for the controlled release of the engineered peptide. Loading of the peptide into CS NPs using the ionic gelation method was confirmed by FTIR and resulted in final particle size, poly-dispersity index and surface charge of 186.5 ± 24.0 nm, 0.26 ± 0.02 and 20.1 ± 0.4 mV respectively. The SEM morphological analysis revealed spherical particles with an average size of 80 ± 5 nm. Peptide loading studies revealed that CS NPs are able to adsorb the peptide as ~70%. The release measurements indicated an initial burst release by 49% after 2 hr and complete release after 80 hr. According to in vitro studies, the loaded peptide was much more toxic for endothelial cells than different cancer cell lines. These results underscore the promise of CS NPs as therapeutics nanosystems and open a perspective for improving the clinical applications of peptide drugs.
Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran.
Sanaz
http://orcid.org/0000-0002-1735-3350
S
Ebrahimi Samani
Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran.
Zahra
Z
Seraj
Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Hossein
H
Naderimanesh
Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Khosro
K
Khajeh
Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran.
Ahmad Reza
AR
Esmaeili Rastaghi
Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.
Taher
T
Droudi
Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.
Peirhosein
P
Kolivand
Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.
Hadi
H
Kazemi
Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran.
S Mohsen
SM
Asghari
D000970:Antineoplastic Agents / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry* / Q000494:pharmacology; D065546:Drug Liberation; D043169:Endostatins / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010455:Peptides / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
D000970:Antineoplastic Agents; D004337:Drug Carriers; D043169:Endostatins; D010455:Peptides; D048271:Chitosan
angiogenesis; chitosan; controlled release; endostatin peptide