Integrated Analysis of LncRNA-mRNA Co-Expression Profiles in Patients with Moyamoya Disease.
Moyamoya disease (MMD) is an idiopathic disease associated with recurrent stroke. However, the pathogenesis of MMD remains unknown. Therefore, we performed long noncoding RNA (lncRNA) and messenger RNA (mRNA) expression profiles in blood samples from MMD patients (N = 15) and healthy controls (N = 10). A total of 880 differentially expressed lncRNAs (3649 probes) and 2624 differentially expressed mRNAs (2880 probes) were obtained from the microarrays of MMD patients and healthy controls (P < 0.05; Fold Change >2.0). Gene ontology (GO) and pathway analyses showed that upregulated mRNAs were enriched for inflammatory response, Toll-like receptor signaling pathway, chemokine signaling pathway and mitogen-activated protein kinase (MAPK) signaling pathway among others, while the downregulated mRNAs were enriched for neurological system process, digestion, drug metabolism, retinol metabolism and others. Our results showed that the integrated analysis of lncRNA-mRNA co-expression networks were linked to inflammatory response, Toll-like signaling pathway, cytokine-cytokine receptor interaction and MAPK signaling pathway. These findings may elucidate the pathogenesis of MMD, and the differentially expressed genes could provide clues to find key components in the MMD pathway.
Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100050, China.|Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Suzhou, 215123, China.|China National Clinical Research Center for Neurological Diseases, Beijing, 100050, China.|Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases, Beijing, 100050, China.
Wen
W
Wang
Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100050, China.|China National Clinical Research Center for Neurological Diseases, Beijing, 100050, China.|Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases, Beijing, 100050, China.
Faliang
F
Gao
Beijing Neurosurgical Institute, Capital Medical University, Beijing, 100050, China.
Zheng
Z
Zhao
Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510280, China.
Haoyuan
H
Wang
Department of Ophthalmology, School of Medicine, Shandong University, Jinan, 250012, China.
Lu
L
Zhang
Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100050, China.|China National Clinical Research Center for Neurological Diseases, Beijing, 100050, China.|Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases, Beijing, 100050, China.
Dong
D
Zhang
Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100050, China.|China National Clinical Research Center for Neurological Diseases, Beijing, 100050, China.|Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases, Beijing, 100050, China.
Yan
Y
Zhang
Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Suzhou, 215123, China.
Qing
Q
Lan
Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100050, China.|China National Clinical Research Center for Neurological Diseases, Beijing, 100050, China.|Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases, Beijing, 100050, China.
Jiangfei
J
Wang
Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100050, China.|Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Suzhou, 215123, China.|China National Clinical Research Center for Neurological Diseases, Beijing, 100050, China.|Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases, Beijing, 100050, China.
Jizong
J
Zhao
D016022:Case-Control Studies; D019295:Computational Biology / Q000379:methods; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D006801:Humans; D008297:Male; D009072:Moyamoya Disease / Q000175:diagnosis / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D059467:Transcriptome
D062085:RNA, Long Noncoding; D012333:RNA, Messenger