Evaluation of polymorphisms in microRNA biosynthesis genes and risk of laryngeal cancer in the Polish population.
MicroRNAs are the largest group of short regulatory RNAs. They regulate genes participating in many physiological and pathological processes. The role of micro RNAs in cancer development is also considerable. Therefore, the aim of this study was to evaluate the relationship between DROSHA (rs6877842) and DGCR8 (rs417309, rs1640299) gene polymorphisms with risk of occurrence of laryngeal cancer. The study included 100 patients and 100 healthy subjects. Genomic DNA was extracted from formalin-fixed, paraffin-embedded tissues. Analysis of the gene polymorphisms was performed using TaqMan SNP Genotyping Assay. The rs417309 AA genotype was found to be correlated with increased risk of larynx cancer. The rs1640299 TG and rs6877842 CG heterozygotes were significantly inversely associated with the presence of larynx cancer. Additionally, rs417309 AA genotype increased the risk of larynx cancer in the T1 stage, and the rs1640299 TG heterozygote occurred more frequently in the control group than those in the T3 and T4 stage. The rs417309 and rs1640299 polymorphisms of the DGCR8 gene as well as rs6877842 of theDROSHAgene might be associated with a risk of laryngeal cancer occurrence in the Polish population.
Otolaryngology Department, Medical University of Warsaw, Warsaw, Poland.
Antoni
A
Bruzgielewicz
Otolaryngology Department, Medical University of Warsaw, Warsaw, Poland.
Ewa
E
Osuch-Wójcikiewicz
Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, Lodz, Poland.
Anna
A
Walczak
Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, Lodz, Poland. alicjanowak87@wp.pl.
Alicja
A
Nowak
Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, Lodz, Poland.
Helen
H
Uczkowski
Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, Lodz, Poland.
Ireneusz
I
Majsterek
D000368:Aged; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D011044:Poland; D020641:Polymorphism, Single Nucleotide; D016601:RNA-Binding Proteins / Q000235:genetics*; D043244:Ribonuclease III / Q000235:genetics*
C474043:DGCR8 protein, human; D035683:MicroRNAs; D016601:RNA-Binding Proteins; C517918:DROSHA protein, human; D043244:Ribonuclease III
 carcinogenesis;  gene polymorphism;  microRNA biosynthesis genes; laryngeal cancer