Design and construction of self-assembling supramolecular protein complexes using artificial and fusion proteins as nanoscale building blocks.
The central goal of nanobiotechnology is to design and construct novel biomaterials of nanometer sizes. In this short review, we describe recent progress of several approaches for designing and creating artificial self-assembling protein complexes and primarily focus on the following biotechnological strategies for using artificial and fusion proteins as nanoscale building blocks: fusion proteins designed for symmetrical self-assembly; three-dimensional domain-swapped oligomers; self-assembling designed coiled-coil peptide modules; metal-directed self-assembling engineered proteins; computationally designed self-assembling de novo proteins; and self-assembling protein nanobuilding blocks (PN-Blocks) using an intermolecularly folded dimeric de novo protein. These state-of-the-art nanobiotechnologies for designing supramolecular protein complexes will facilitate the development of novel functional nanobiomaterials.
Department of Applied Biology, Faculty of Textile Science & Technology, Shinshu University, 3-15-1 Tokida, Ueda, Nagano 386-8567, Japan; Department of Bioscience and Textile Technology, Interdisciplinary Graduate School of Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda, Nagano 386-8567, Japan.
Naoya
N
Kobayashi
Department of Applied Biology, Faculty of Textile Science & Technology, Shinshu University, 3-15-1 Tokida, Ueda, Nagano 386-8567, Japan; Department of Bioscience and Textile Technology, Interdisciplinary Graduate School of Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda, Nagano 386-8567, Japan; Department of Supramolecular Complexes, Research Center for Fungal & Microbial Dynamism, Shinshu University, 8304 Minamiminowa, Kamiina, Nagano 399-4598, Japan; Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan; Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan. Electronic address: rarai@shinshu-u.ac.jp.
Ryoichi
R
Arai
D001709:Biotechnology; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D010455:Peptides / Q000737:chemistry; D015202:Protein Engineering / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000737:chemistry*
D010455:Peptides; D011993:Recombinant Fusion Proteins