Aryl hydrocarbon receptor (AhR) rs2066853 gene polymorphism association with infertile oligoasthenoteratozoospermic men and seminal oxidative stress.
This study aimed to assess the association between aryl hydrocarbon receptor (AhR) rs2066853 gene polymorphism with infertile oligoasthenoteratozoospermic (OAT) men and seminal oxidative stress (OS). A total of 170 Egyptian men were allocated according to their semen analysis into fertile normozoospermic controls (n = 50) and infertile OAT men (n = 120). They were subjected to history taking, clinical examination, semen analysis, estimation of seminal glutathione peroxidase (GPx), and malondialdehyde (MDA). AhR rs2066853 gene polymorphism was identified in the blood by PCR-RFLP. Comparing infertile OAT men with fertile controls, AhR rs2066853 genotypes showed decreased prevalence for wild homozygous genotype GG (35.8 vs 56%) and for heterozygous genotype GA (17.5 vs 30%) and an increased prevalence for homozygous genotype AA (46.7 vs 14%). Distribution of alleles of AhR rs2066853 among OAT men compared with fertile men showed decreased prevalence of G allele (44.6 vs 71%) and an increased prevalence of A allele (55.4 vs 29%). Seminal MDA demonstrated significant increase whereas seminal GPx demonstrated significant decrease in cases with AA and GA/AA genotypes compared to cases with GG genotype. It is concluded that there is a significant association between AhR rs2066853 genotype polymorphism with decreased sperm parameters as well as increased seminal oxidative stress in infertile OAT men.
Department of Andrology, Sexology& STIs, Faculty of Medicine, Cairo University, Cairo, 11562, Egypt. taymour1155@link.net.
Taymour
T
Mostafa
Department of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt.
Hanan
H
Fouad
Department of Andrology, Sexology& STIs, Faculty of Medicine, Beni Suef University, Beni Suef, Egypt.
Nashaat
N
Nabil
Department of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt.
Laila
L
Rashed
Department of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt.
Dina
D
Sabry
Department of Clinical Pathology, Faculty of Medicine, Beni Suef University, Beni Suef, Egypt.
Khadiga
K
Abougabal
Department of Andrology, Sexology & STIs, Faculty of Medicine, Beni Suef University, Beni Suef, Egypt.
Bolis S
BS
Gendy
D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D004534:Egypt; D005298:Fertility; D005838:Genotype; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D018384:Oxidative Stress / Q000235:genetics*; D011110:Polymorphism, Genetic; D018336:Receptors, Aryl Hydrocarbon / Q000235:genetics*; D012661:Semen / Q000378:metabolism; D055101:Semen Analysis; D013094:Spermatozoa / Q000378:metabolism
D018336:Receptors, Aryl Hydrocarbon; D008315:Malondialdehyde; D005979:Glutathione Peroxidase
Aryl hydrocarbon receptor; Male infertility; Oligoasthenoteratozoospermia; Oxidative stress; Polymorphism; rs2066853