Anti-Osteoclastic Activity of Artemisia capillaris Thunb. Extract Depends upon Attenuation of Osteoclast Differentiation and Bone Resorption-Associated Acidification Due to Chlorogenic Acid, Hyperoside, and Scoparone.
The present study attempts to elucidate the anti-osteoporotic activity ofArtemisia capillarisThunb. in the form of anti-osteoclastic effect and responsible bioactive compounds. The contents of chlorogenic acid, caffeic acid, hyperoside, isoquercitrin, isochlorogenic acid A, and scoparone inArtemisia capillarishydroethanolic extract (ACHE) were 38.53, 0.52, 4.07, 3.03, 13.90, and 6.59 mg/g, respectively. ACHE diminished osteoclast differentiation and bone resorption due to chlorogenic acid, hyperoside, and scoparone. In addition, ACHE attenuated acidification as well as reducing tumor necrosis factor receptor-associated factor 6 (TRAF6) expression and its association with vacuolar H⁺-adenosine triphosphatase (V-ATPase). Furthermore, chlorogenic acid, hyperoside, and scoparone fromA. capillarisabrogated the association of V-ATPase with TRAF6, suggesting that the blockage of bone resorption byA. capillariswas partially mediated by reducing acidification through down-regulating interaction of V-ATPase with TRAF6 due to scoparone as well as chlorogenic acid and hyperoside. These results imply that the anti-osteoclastic effect ofA. capillaristhrough down-regulating osteoclast differentiation and bone resorption may contribute to its anti-osteoporotic effect.
Department of Food and Nutrition, Hannam University, Daejeon 34430, Korea. ggamannom03@nate.com.
Sang-Hyun
SH
Lee
Department of Food and Nutrition, Hannam University, Daejeon 34430, Korea. seembeeks@hanmail.net.
Jung-Yun
JY
Lee
Department of Food and Nutrition, Hannam University, Daejeon 34430, Korea. youngk@hnu.kr.
Young-In
YI
Kwon
Department of Food and Nutrition, Hannam University, Daejeon 34430, Korea. haedong@hnu.kr.
Hae-Dong
HD
Jang
D000818:Animals; D019306:Artemisia / Q000737:chemistry*; D001862:Bone Resorption; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D002726:Chlorogenic Acid / Q000737:chemistry; D003374:Coumarins / Q000737:chemistry; D015870:Gene Expression; D051379:Mice; D015394:Molecular Structure; D010010:Osteoclasts / Q000166:cytology* / Q000502:physiology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011485:Protein Binding; D011794:Quercetin / Q000031:analogs & derivatives / Q000737:chemistry; D048029:TNF Receptor-Associated Factor 6 / Q000235:genetics / Q000378:metabolism; D025262:Vacuolar Proton-Translocating ATPases / Q000378:metabolism
D003374:Coumarins; D010936:Plant Extracts; D048029:TNF Receptor-Associated Factor 6; D002726:Chlorogenic Acid; C021304:hyperoside; D011794:Quercetin; D025262:Vacuolar Proton-Translocating ATPases; C018145:scoparone
Artemisia capillaris; acidification; bone resorption; osteoclast differentiation