Glutathione-S-transferase P1 may predispose children to a decline in pulmonary function after stem cell transplant.
Pulmonary complications after hematopoietic stem cell transplant (SCT) are associated with increased mortality. Genetic markers for those at risk for pulmonary impairment post-SCT have not been widely investigated.



Forty-nine patients were retrospectively selected from a single institution's biorepository with linked clinical data. All subjects performed pre-SCT PFTs. Genotyping was conducted using the Infinium Exome-24 BeadChip. Four single nucleotide polymorphisms (SNPs) were selected (rs1800871, rs1695, rs1800629, rs12477314) and evaluated for association with PFT parameters as change over time from baseline. Associations between SNPs and PFT parameters were assessed and adjusted for the following confounding variables: age, gender, and race.



Using the recessive genetic model, patients with one or two minor alleles for the glutathione S-transferase P1 (GSTP1) SNP rs1695 had a lower decline in FEV1and FEF25-75at 1-year post-SCT compared to patients who were homozygous for the ancestral allele (adjusted P-values <0.01 and 0.02, respectively). No other SNPs were significantly associated with other PFT parameters.



Our findings suggest that GSTP1 genotype may be associated with lung function during the first year post-SCT. Identifying and investigating genes that predispose patients to pulmonary complications after SCT may allow for more personalized patient management based on pre-emptive genetic testing. The glutathione S-transferase gene merits further investigation.
Department of Pediatrics, Indiana University School of Medicine, Indianapolis, Indiana.
Julie
J
Stark
Department of Hematology/Oncology, Indiana University School of Medicine, Indianapolis, Indiana.
Jamie
J
Renbarger
Department of Biostatics, Indiana University School of Medicine, Indianapolis, Indiana.
James
J
Slaven
Department of Biostatics, Indiana University School of Medicine, Indianapolis, Indiana.
Zhangsheng
Z
Yu
Department of Hematology/Oncology, Indiana University School of Medicine, Indianapolis, Indiana.
Jenny
J
Then
Department of Hematology/Oncology, Indiana University School of Medicine, Indianapolis, Indiana.
Jodi
J
Skiles
Department of Pediatrics, Section of Pediatric Pulmonology, Allergyand Sleep Medicine, Indiana University School of Medicine, Indianapolis, Indiana.
Stephanie
S
Davis
D000293:Adolescent; D002648:Child; D005260:Female; D020022:Genetic Predisposition to Disease; D005820:Genetic Testing; D005838:Genotype; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide; D012129:Respiratory Function Tests; D012189:Retrospective Studies; D033581:Stem Cell Transplantation
C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi
biomarkers; genetics/genome-wide association studies (GWAS); lung pathology; pulmonary function testing (PFT)