DDX50 inhibits the replication of dengue virus 2 by upregulating IFN-β production.
Dengue virus (DENV) infects approximately 390 million people per year, and each of the four DENV serotypes (DENV-1, DENV-2, DENV-3, and DENV-4) is capable of causing infection. At present, there is no antiviral drug available for the treatment of DENV. Several DExD/H-box helicases have been shown to be involved in the antiviral immune response or viral replication. In the present study, we investigated the role of DDX50 in DENV-2 RNA replication. Our data showed that the level of DENV-2 RNA increased in DDX50 knockdown cells during an early stage of viral infection and decreased in DDX50-overexpressing cells. DDX50, in conjunction with RIG-I and MDA5, upregulated the production of IFN-β in infected cells through an additive effect on the IFN-β promoter. Furthermore, transcription of several IFN-stimulated genes was increased in DDX50-overexpressing cells infected with DENV-2. These results provide evidence that DDX50 negatively regulates DENV-2 replication during the early stages of infection by inducing IFN-β production.
The Department of Microbiology, School of Preclinical Medicine, The Fourth Military Medical University, No.169, Changle West Road, Xian, 710032, Shaanxi, China.
Peijun
P
Han
The Department of Microbiology, School of Preclinical Medicine, The Fourth Military Medical University, No.169, Changle West Road, Xian, 710032, Shaanxi, China.
Wei
W
Ye
The Department of Microbiology, School of Preclinical Medicine, The Fourth Military Medical University, No.169, Changle West Road, Xian, 710032, Shaanxi, China.
Xin
X
Lv
The Department of Microbiology, School of Preclinical Medicine, The Fourth Military Medical University, No.169, Changle West Road, Xian, 710032, Shaanxi, China.
Hongwei
H
Ma
The Department of Microbiology, School of Preclinical Medicine, The Fourth Military Medical University, No.169, Changle West Road, Xian, 710032, Shaanxi, China.
Daihui
D
Weng
The Department of Microbiology, School of Preclinical Medicine, The Fourth Military Medical University, No.169, Changle West Road, Xian, 710032, Shaanxi, China.
Yangchao
Y
Dong
The Department of Microbiology, School of Preclinical Medicine, The Fourth Military Medical University, No.169, Changle West Road, Xian, 710032, Shaanxi, China.
Linfeng
L
Cheng
The Department of Microbiology, School of Preclinical Medicine, The Fourth Military Medical University, No.169, Changle West Road, Xian, 710032, Shaanxi, China.
Hesong
H
Chen
The Department of Microbiology, School of Preclinical Medicine, The Fourth Military Medical University, No.169, Changle West Road, Xian, 710032, Shaanxi, China.
Liang
L
Zhang
The Department of Microbiology, School of Preclinical Medicine, The Fourth Military Medical University, No.169, Changle West Road, Xian, 710032, Shaanxi, China.
Zhikai
Z
Xu
The Department of Microbiology, School of Preclinical Medicine, The Fourth Military Medical University, No.169, Changle West Road, Xian, 710032, Shaanxi, China. yflei@fmmu.edu.cn.
Yingfeng
Y
Lei
The Department of Microbiology, School of Preclinical Medicine, The Fourth Military Medical University, No.169, Changle West Road, Xian, 710032, Shaanxi, China. flzhang@fmmu.edu.cn.
Fanglin
F
Zhang
D002460:Cell Line; D000071457:DEAD Box Protein 58 / Q000235:genetics; D053487:DEAD-box RNA Helicases / Q000235:genetics* / Q000502:physiology*; D004261:DNA Replication; D003716:Dengue Virus / Q000276:immunology / Q000502:physiology*; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006801:Humans; D007113:Immunity, Innate; D000072640:Interferon-Induced Helicase, IFIH1 / Q000235:genetics; D016899:Interferon-beta / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D011971:Receptors, Immunologic; D015854:Up-Regulation; D014779:Virus Replication
D011971:Receptors, Immunologic; D016899:Interferon-beta; C448980:DDX50 protein, human; C452820:RIGI protein, human; C452815:IFIH1 protein, human; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases; D000072640:Interferon-Induced Helicase, IFIH1