ABA Suppresses Root Hair Growth via the OBP4 Transcriptional Regulator.
Plants modify organ growth and tune morphogenesis in response to various endogenous and environmental cues. At the cellular level, organ growth is often adjusted by alterations in cell growth, but the molecular mechanisms underlying this control remain poorly understood. In this study, we identify the DNA BINDING WITH ONE FINGER (DOF)-type transcription regulator OBF BINDING PROTEIN4 (OBP4) as a repressor of cell growth. Ectopic expression ofOBP4in Arabidopsis (Arabidopsis thaliana) inhibits cell growth, resulting in severe dwarfism and the repression of genes involved in the regulation of water transport, root hair development, and stress responses. Among the basic helix-loop-helix transcription factors known to control root hair growth, OBP4 binds theROOT HAIR DEFECTIVE6-LIKE2(RSL2) promoter to repress its expression. The accumulation of OBP4 proteins is detected in expanding root epidermal cells, and its expression level is increased by the application of abscisic acid (ABA) at concentrations sufficient to inhibit root hair growth. ABA-dependent induction ofOBP4is associated with the reduced expression ofRSL2Furthermore, ectopic expression ofOBP4or loss ofRSL2function results in ABA-insensitive root hair growth. Taken together, our results suggest that OBP4-mediated transcriptional repression ofRSL2contributes to the ABA-dependent inhibition of root hair growth in Arabidopsis.
RIKEN CSRS, Yokohama 230-0045, Japan (B.R., A.K., C.B., A.I., M.S., M.M., K.S.).|Max-Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany (S.S., C.K.).|Graduate School of Science and Engineering (M.I.) and Institute for Environmental Science and Technology (M.O.-T.), Saitama University, Saitama 338-8570, Japan.|Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8566, Japan (M.I., N.M., M.O.-T.); and.|Institute of Plant Biology, Biological Research Center of the Hungarian Academy of Sciences, H-6724 Szeged, Hungary (C.K.).
Bart
0000-0003-3651-9579
B
Rymen
RIKEN CSRS, Yokohama 230-0045, Japan (B.R., A.K., C.B., A.I., M.S., M.M., K.S.).|Max-Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany (S.S., C.K.).|Graduate School of Science and Engineering (M.I.) and Institute for Environmental Science and Technology (M.O.-T.), Saitama University, Saitama 338-8570, Japan.|Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8566, Japan (M.I., N.M., M.O.-T.); and.|Institute of Plant Biology, Biological Research Center of the Hungarian Academy of Sciences, H-6724 Szeged, Hungary (C.K.).
Ayako
A
Kawamura
RIKEN CSRS, Yokohama 230-0045, Japan (B.R., A.K., C.B., A.I., M.S., M.M., K.S.).|Max-Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany (S.S., C.K.).|Graduate School of Science and Engineering (M.I.) and Institute for Environmental Science and Technology (M.O.-T.), Saitama University, Saitama 338-8570, Japan.|Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8566, Japan (M.I., N.M., M.O.-T.); and.|Institute of Plant Biology, Biological Research Center of the Hungarian Academy of Sciences, H-6724 Szeged, Hungary (C.K.).
Sabine
S
Schäfer
RIKEN CSRS, Yokohama 230-0045, Japan (B.R., A.K., C.B., A.I., M.S., M.M., K.S.).|Max-Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany (S.S., C.K.).|Graduate School of Science and Engineering (M.I.) and Institute for Environmental Science and Technology (M.O.-T.), Saitama University, Saitama 338-8570, Japan.|Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8566, Japan (M.I., N.M., M.O.-T.); and.|Institute of Plant Biology, Biological Research Center of the Hungarian Academy of Sciences, H-6724 Szeged, Hungary (C.K.).
Christian
C
Breuer
RIKEN CSRS, Yokohama 230-0045, Japan (B.R., A.K., C.B., A.I., M.S., M.M., K.S.).|Max-Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany (S.S., C.K.).|Graduate School of Science and Engineering (M.I.) and Institute for Environmental Science and Technology (M.O.-T.), Saitama University, Saitama 338-8570, Japan.|Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8566, Japan (M.I., N.M., M.O.-T.); and.|Institute of Plant Biology, Biological Research Center of the Hungarian Academy of Sciences, H-6724 Szeged, Hungary (C.K.).
Akira
0000-0003-3294-7939
A
Iwase
RIKEN CSRS, Yokohama 230-0045, Japan (B.R., A.K., C.B., A.I., M.S., M.M., K.S.).|Max-Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany (S.S., C.K.).|Graduate School of Science and Engineering (M.I.) and Institute for Environmental Science and Technology (M.O.-T.), Saitama University, Saitama 338-8570, Japan.|Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8566, Japan (M.I., N.M., M.O.-T.); and.|Institute of Plant Biology, Biological Research Center of the Hungarian Academy of Sciences, H-6724 Szeged, Hungary (C.K.).
Michitaro
M
Shibata
RIKEN CSRS, Yokohama 230-0045, Japan (B.R., A.K., C.B., A.I., M.S., M.M., K.S.).|Max-Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany (S.S., C.K.).|Graduate School of Science and Engineering (M.I.) and Institute for Environmental Science and Technology (M.O.-T.), Saitama University, Saitama 338-8570, Japan.|Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8566, Japan (M.I., N.M., M.O.-T.); and.|Institute of Plant Biology, Biological Research Center of the Hungarian Academy of Sciences, H-6724 Szeged, Hungary (C.K.).
Miho
0000-0002-2713-5293
M
Ikeda
RIKEN CSRS, Yokohama 230-0045, Japan (B.R., A.K., C.B., A.I., M.S., M.M., K.S.).|Max-Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany (S.S., C.K.).|Graduate School of Science and Engineering (M.I.) and Institute for Environmental Science and Technology (M.O.-T.), Saitama University, Saitama 338-8570, Japan.|Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8566, Japan (M.I., N.M., M.O.-T.); and.|Institute of Plant Biology, Biological Research Center of the Hungarian Academy of Sciences, H-6724 Szeged, Hungary (C.K.).
Nobutaka
N
Mitsuda
RIKEN CSRS, Yokohama 230-0045, Japan (B.R., A.K., C.B., A.I., M.S., M.M., K.S.).|Max-Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany (S.S., C.K.).|Graduate School of Science and Engineering (M.I.) and Institute for Environmental Science and Technology (M.O.-T.), Saitama University, Saitama 338-8570, Japan.|Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8566, Japan (M.I., N.M., M.O.-T.); and.|Institute of Plant Biology, Biological Research Center of the Hungarian Academy of Sciences, H-6724 Szeged, Hungary (C.K.).
Csaba
0000-0003-4422-9993
C
Koncz
RIKEN CSRS, Yokohama 230-0045, Japan (B.R., A.K., C.B., A.I., M.S., M.M., K.S.).|Max-Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany (S.S., C.K.).|Graduate School of Science and Engineering (M.I.) and Institute for Environmental Science and Technology (M.O.-T.), Saitama University, Saitama 338-8570, Japan.|Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8566, Japan (M.I., N.M., M.O.-T.); and.|Institute of Plant Biology, Biological Research Center of the Hungarian Academy of Sciences, H-6724 Szeged, Hungary (C.K.).
Masaru
M
Ohme-Takagi
RIKEN CSRS, Yokohama 230-0045, Japan (B.R., A.K., C.B., A.I., M.S., M.M., K.S.).|Max-Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany (S.S., C.K.).|Graduate School of Science and Engineering (M.I.) and Institute for Environmental Science and Technology (M.O.-T.), Saitama University, Saitama 338-8570, Japan.|Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8566, Japan (M.I., N.M., M.O.-T.); and.|Institute of Plant Biology, Biological Research Center of the Hungarian Academy of Sciences, H-6724 Szeged, Hungary (C.K.).
Minami
M
Matsui
RIKEN CSRS, Yokohama 230-0045, Japan (B.R., A.K., C.B., A.I., M.S., M.M., K.S.); keiko.sugimoto@riken.jp.|Max-Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany (S.S., C.K.); keiko.sugimoto@riken.jp.|Graduate School of Science and Engineering (M.I.) and Institute for Environmental Science and Technology (M.O.-T.), Saitama University, Saitama 338-8570, Japan; keiko.sugimoto@riken.jp.|Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8566, Japan (M.I., N.M., M.O.-T.); and keiko.sugimoto@riken.jp.|Institute of Plant Biology, Biological Research Center of the Hungarian Academy of Sciences, H-6724 Szeged, Hungary (C.K.) keiko.sugimoto@riken.jp.
Keiko
0000-0002-9209-8230
K
Sugimoto
D000040:Abscisic Acid / Q000494:pharmacology*; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D018613:Microscopy, Confocal; D009154:Mutation; D019441:Plant Epidermis / Q000235:genetics / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000235:genetics* / Q000254:growth & development; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D020133:Reverse Transcriptase Polymerase Chain Reaction
D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C000623373:OBP4 protein, Arabidopsis; D010937:Plant Growth Regulators; C000623374:RSL2 protein, Arabidopsis; D000040:Abscisic Acid