MicroRNA-100 is involved in shrimp immune response to white spot syndrome virus (WSSV) and Vibrio alginolyticus infection.
In this study, we discovered that shrimp miR-100 was up-regulated at 24 h after WSSV or Vibrio alginolyticus infection, confirming its participation in the innate immune system of shrimp. The anti-miRNA oligonucleotide (AMO-miR-100) was applied to inhibit the expression of miR-100. After AMO-miR-100 treatment, the shrimp was challenged with WSSV or V. alginolyticus. The knockdown of miR-100 expression decreased the mortality of WSSV-infected shrimp from 24 h to 72 h post-infection and enhanced the mortality of V. alginolyticus-infected shrimp significantly. The knockdown of miR-100 affected phenoloxidase (PO) activity, superoxide dismutase (SOD) activity and total hemocyte count (THC) after the infection with WSSV or V. alginolyticus, indicating a regulative role of miR-100 in the immune potential of shrimp in the response to WSSV or V. alginolyticus infection. The knockdown of miR-100 induced the apoptosis of shrimp hemocytes, and V. alginolyticus + AMO-miR-100 treatment caused more hemocyte apoptosis than V. alginolyticus treatment. The miR-100 influenced also the morphology of shrimp hemocytes and regulated the phagocytosis of WSSV or V. alginolyticus. Thus, we concluded that miR-100 may promote the anti-Vibrio immune response of shrimp through regulating apoptosis, phagocytosis and PO activity and affects the progression of WSSV infection at a certain level.
College of Animal Science and Technology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China.
Zhi
Z
Wang
College of Animal Science and Technology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China.
Fei
F
Zhu
D000818:Animals; D017209:Apoptosis; D048430:Cell Shape; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D006434:Hemocytes / Q000166:cytology; D006458:Hemolymph / Q000201:enzymology; D007109:Immunity / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D014442:Monophenol Monooxygenase / Q000378:metabolism; D009841:Oligonucleotides / Q000378:metabolism; D033561:Penaeidae / Q000201:enzymology / Q000235:genetics / Q000276:immunology* / Q000821:virology*; D010587:Phagocytosis; D013482:Superoxide Dismutase / Q000378:metabolism; D014735:Vibrio Infections / Q000235:genetics* / Q000276:immunology* / Q000473:pathology; D044149:Vibrio alginolyticus / Q000502:physiology*; D046848:White spot syndrome virus 1 / Q000502:physiology*
D035683:MicroRNAs; D009841:Oligonucleotides; D014442:Monophenol Monooxygenase; D013482:Superoxide Dismutase