Effects of ferulic acid on oxidative stress, heat shock protein 70, connexin 43, and monoamines in the hippocampus of pentylenetetrazole-kindled rats.
The present study investigated the effects of ferulic acid (FA) on pentylenetetrazole (PTZ)-induced seizures, oxidative stress markers (malondialdehyde (MDA), catalase, and reduced glutathione (GSH)), connexin (Cx) 43, heat shock protein 70 (Hsp 70), and monoamines (serotonin (5-HT) and norepinephrine (NE)) levels in a rat model of PTZ-induced kindling. Sixty Sprague Dawley rats were divided into 5 equal groups: (a) normal group; (b) FA group: normal rats received FA at a dose of 40 mg/kg daily; (c) PTZ group: normal rats received PTZ at a dose of 50 mg/kg i.p. on alternate days for 15 days; (d) FA-before group: treatment was the same as for the PTZ group, except rats received FA; and (e) FA-after group: rats received FA from sixth dose of PTZ. PTZ caused a significant increase in MDA, Cx43, and Hsp70 along with a significant decrease in GSH, 5-HT, and NE levels and CAT activity in the hippocampus (p < 0.05). Pre- and post-treatment with FA caused significant improvement in behavioral parameters, MDA, CAT, GSH, 5-HT, NE, Cx43 expression, and Hsp70 expression in the hippocampal region (p < 0.05). We conclude that FA has neuroprotective effects in PTZ-induced epilepsy, which might be due to attenuation of oxidative stress and Cx43 expression and upregulation of neuroprotective Hsp70 and neurotransmitters (5-HT and NE).
a Faculty of Medicine, Mansoura University, El Gomhoria Street, Mansoura, Egypt.
Abdelaziz M
AM
Hussein
a Faculty of Medicine, Mansoura University, El Gomhoria Street, Mansoura, Egypt.
Khaled M
KM
Abbas
b Chemistry and Drug Metabolism, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Biomedical Research Center, 251 Bayview Boulevard, Baltimore, MD 21224, USA.
Osama A
OA
Abulseoud
c Faculty of Medicine, Kafrelsheikh University, El Gish Street, Kafr El Sheikh, Egypt.
El-Hussainy M A
EMA
El-Hussainy
D000818:Animals; D015306:Biogenic Monoamines / Q000378:metabolism*; D056654:CA3 Region, Hippocampal / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D002374:Catalase / Q000378:metabolism; D018031:Connexin 43 / Q000378:metabolism*; D003373:Coumaric Acids / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007696:Kindling, Neurologic / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010433:Pentylenetetrazole / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D012701:Serotonin / Q000378:metabolism
D015306:Biogenic Monoamines; D018031:Connexin 43; D003373:Coumaric Acids; D018840:HSP70 Heat-Shock Proteins; D012701:Serotonin; D008315:Malondialdehyde; C004999:ferulic acid; D002374:Catalase; D010433:Pentylenetetrazole; D009638:Norepinephrine
Cx43; acide férulique; embrasement; ferulic acid; heat shock protein 70; kindling; norepinephrine; norépinéphrine; pentylenetetrazole; pentylènetétrazole; protéine de choc thermique 70; rats; serotonin; sérotonine