Assays for Acetylation and Other Acylations of Lysine Residues.
Lysine acetylation refers to addition of an acetyl moiety to the epsilon-amino group of a lysine residue and is important for regulating protein functions in various organisms from bacteria to humans. This is a reversible and precisely controlled covalent modification that either serves as an on/off switch or participates in a codified manner with other post-translational modifications to regulate different cellular and developmental processes in normal and pathological states. This unit describes methods for in vitro and in vivo determination of lysine acetylation. Such methods can be easily extended for analysis of other acylations (such as propionylation, butyrylation, crotonylation, and succinylation) that are also present in histones and many other proteins. © 2017 by John Wiley & Sons, Inc.
Goodman Cancer Research Center and Department of Medicine, McGill University, Montreal, Quebec, Canada.
Nadine
N
Pelletier
Goodman Cancer Research Center and Department of Medicine, McGill University, Montreal, Quebec, Canada.
Serge
S
Grégoire
Goodman Cancer Research Center and Department of Medicine, McGill University, Montreal, Quebec, Canada.
Xiang-Jiao
XJ
Yang
D000107:Acetylation; D000215:Acylation; D000818:Animals; D000906:Antibodies / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D057809:HEK293 Cells; D051548:Histone Acetyltransferases / Q000737:chemistry / Q000378:metabolism; D006657:Histones / Q000737:chemistry; D006801:Humans; D008239:Lysine / Q000737:chemistry*; D000070837:Lysine Acetyltransferases / Q000737:chemistry / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry*; D011817:Rabbits
D000906:Antibodies; D006657:Histones; D011506:Proteins; D000070837:Lysine Acetyltransferases; D051548:Histone Acetyltransferases; D008239:Lysine
butyrylation; chromatin; crotonylation; glutarylation; histone acetyltransferase; lysine acetylation; metabolic enzymes; propionylation; transcription factor; tubulin