Photodynamic therapy monitoring with optical coherence angiography.
Photodynamic therapy (PDT) is a promising modern approach for cancer therapy with low normal tissue toxicity. This study was focused on a vascular-targeting Chlorine E6 mediated PDT. A new angiographic imaging approach known as M-mode-like optical coherence angiography (MML-OCA) was able to sensitively detect PDT-induced microvascular alterations in the mouse ear tumour model CT26. Histological analysis showed that the main mechanisms of vascular PDT was thrombosis of blood vessels and hemorrhage, which agrees with angiographic imaging by MML-OCA. Relationship between MML-OCA-detected early microvascular damage post PDT (within 24 hours) and tumour regression/regrowth was confirmed by histology. The advantages of MML-OCA such as direct image acquisition, fast processing, robust and affordable system opto-electronics, and label-free high contrast 3D visualization of the microvasculature suggest attractive possibilities of this method in practical clinical monitoring of cancer therapies with microvascular involvement.
Nizhny Novgorod State Medical Academy, Minina Square 10/1, 603005 Nizhny Novgorod, Russia.
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Sirotkina
Nizhny Novgorod State Medical Academy, Minina Square 10/1, 603005 Nizhny Novgorod, Russia.|Institute of Applied Physics Russian Academy of Sciences, Ulyanova Street 46, 603950 Nizhny Novgorod, Russia.
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LA
Matveev
Nizhny Novgorod State Medical Academy, Minina Square 10/1, 603005 Nizhny Novgorod, Russia.
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Shirmanova
Nizhny Novgorod State Medical Academy, Minina Square 10/1, 603005 Nizhny Novgorod, Russia.|Institute of Applied Physics Russian Academy of Sciences, Ulyanova Street 46, 603950 Nizhny Novgorod, Russia.
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VY
Zaitsev
Nizhny Novgorod State Medical Academy, Minina Square 10/1, 603005 Nizhny Novgorod, Russia.
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Buyanova
Nizhny Novgorod State Medical Academy, Minina Square 10/1, 603005 Nizhny Novgorod, Russia.
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Elagin
Nizhny Novgorod State Medical Academy, Minina Square 10/1, 603005 Nizhny Novgorod, Russia.|Institute of Applied Physics Russian Academy of Sciences, Ulyanova Street 46, 603950 Nizhny Novgorod, Russia.
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Gelikonov
Nizhny Novgorod State Medical Academy, Minina Square 10/1, 603005 Nizhny Novgorod, Russia.
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Kuznetsov
Nizhny Novgorod State Medical Academy, Minina Square 10/1, 603005 Nizhny Novgorod, Russia.
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EB
Kiseleva
Nizhny Novgorod State Medical Academy, Minina Square 10/1, 603005 Nizhny Novgorod, Russia.|Institute of Applied Physics Russian Academy of Sciences, Ulyanova Street 46, 603950 Nizhny Novgorod, Russia.
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AA
Moiseev
Republican Clinical Oncology Dispensary, Gladkova F. Street 23, 428000 Cheboksary, Russia.
S V
SV
Gamayunov
Nizhny Novgorod State Medical Academy, Minina Square 10/1, 603005 Nizhny Novgorod, Russia.
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EV
Zagaynova
Nizhny Novgorod State Medical Academy, Minina Square 10/1, 603005 Nizhny Novgorod, Russia.
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Feldchtein
Nizhny Novgorod State Medical Academy, Minina Square 10/1, 603005 Nizhny Novgorod, Russia.|University of Toronto and University Health Network, 610 University Ave., Toronto, Ontario, M5G 2M9, Canada.
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Vitkin
Nizhny Novgorod State Medical Academy, Minina Square 10/1, 603005 Nizhny Novgorod, Russia.
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ND
Gladkova
D000792:Angiography; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D005453:Fluorescence; D008807:Mice, Inbred BALB C; D009369:Neoplasms / Q000000981:diagnostic imaging* / Q000188:drug therapy*; D038761:Photobleaching; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology / Q000627:therapeutic use; D041623:Tomography, Optical Coherence; D047368:Tumor Burden / Q000187:drug effects
D017319:Photosensitizing Agents