Characterization ofPseudomonas syringaepv.syringae, Causal Agent of Citrus Blast of Mandarin in Montenegro.
Citrus blast caused by bacteriumPseudomonas syringaeis a very important disease of citrus occuring in many areas of the world, but with few data about genetic structure of the pathogen involved. Considering the above fact, this study reports genetic characterization of 43P. syringaeisolates obtained from plant tissue displaying citrus blast symptoms on mandarin (Citrus reticulata) in Montenegro, using multilocus sequence analysis ofgyrB,rpoD, andgap1gene sequences. Gene sequences from a collection of 54 reference pathotype strains ofP. syringaefrom the Plant Associated and Environmental Microbes Database (PAMDB) was used to establish a genetic relationship with our isolates obtained from mandarin. Phylogenetic analyses ofgyrB,rpoD, andgap1gene sequences showed thatP. syringaepv.syringaecauses citrus blast in mandarin in Montenegro, and belongs to genomospecies 1. Genetic homogeneity of isolates suggested that the Montenegrian population might be clonal which indicates a possible common source of infection. These findings may assist in further epidemiological studies of this pathogen and for determining mandarin breeding strategies forP. syringaecontrol.
Institute for Plant Protection and Environment, Belgrade 11040, Serbia.
Žarko
Ž
Ivanović
Biotechnical Faculty, University of Podgorica, Podgorica 81000, Montenegro.
Tatjana
T
Perović
Institute for Plant Protection and Environment, Belgrade 11040, Serbia.
Tatjana
T
Popović
Scholar of Ministry of Education, Science and Technological Development of the Republic of Serbia, Department of Plant Disease, Institute for Plant Protection and Environment, Belgrade 11040, Serbia.
Jovana
J
Blagojević
Institute for Plant Protection and Environment, Belgrade 11040, Serbia.
Nenad
N
Trkulja
Biotechnical Faculty, University of Podgorica, Podgorica 81000, Montenegro.
Snježana
S
Hrnčić
Citrus reticulata; bacteria; multilocus sequence analysis; pathogen; phylogeny