2,4-Dichlorophenol Enzymatic Removal and Its Kinetic Study Using Horseradish Peroxidase Crosslinked to Nano Spray-Dried Poly(Lactic-Co-Glycolic Acid) Fine Particles.
Horseradish peroxidase (HRP) catalyzes the oxidation of aromatic compounds by hydrogen peroxide via insoluble polymer formation, which can be precipitated from the wastewater. For HRP immobilization, poly(lactic-co-glycolic acid) (PLGA) fine carrier supports were produced by using the Nano Spray Dryer B-90. Immobilized HRP was used to remove the persistent 2,4-dichlorophenol from model wastewater. Both extracted (9-16 U/g) and purified HRP (11-25 U/g) retained their activity to a high extent after crosslinking to the PLGA particles. The immobilized enzyme activity was substantially higher in both the acidic and the alkaline pH regions compared with the free enzyme. Optimally, 98% of the 2,4-dichlorophenol could be eliminated using immobilized HRP due to catalytic removal and partly to adsorption on the carrier supports. Immobilized enzyme kinetics for 2,4-dichlorophenol elimination was studied for the first time, and it could be concluded that competitive product inhibition took place.
Doctoral School of Molecular and Nanotechnologies, Research Institute of Chemical and Process Engineering, University of Pannonia, H-8200 Veszprém, Hungary.
Laura Amina
LA
Dahili
Doctoral School of Molecular and Nanotechnologies, Research Institute of Chemical and Process Engineering, University of Pannonia, H-8200 Veszprém, Hungary.
Endre
E
Nagy
Doctoral School of Molecular and Nanotechnologies, Research Institute of Chemical and Process Engineering, University of Pannonia, H-8200 Veszprém, Hungary.|Department of Medicine 1, Goethe University Frankfurt, 60590 Frankfurt am Main, Germany.|Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, H-1117 Budapest, Hungary.
Tivadar
T
Feczkó
D000327:Adsorption; D031215:Armoracia / Q000201:enzymology; D002384:Catalysis; D002733:Chlorophenols / Q000378:metabolism*; D057075:Enzyme Assays; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D019344:Lactic Acid / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010936:Plant Extracts / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013696:Temperature; D062065:Wastewater; D018508:Water Purification
D002733:Chlorophenols; D004800:Enzymes, Immobilized; D006735:Horseradish Peroxidase; D019344:Lactic Acid; D010936:Plant Extracts; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D062065:Wastewater; C004762:2,4-dichlorophenol
2,4-dichlorophenol substrate; Horseradish peroxidase; enzyme kinetics; immobilization