Macroporous graphene capped Fe3O4for amplified electrochemiluminescence immunosensing of carcinoembryonic antigen detection based on CeO2@TiO2.
A novel electrochemiluminescence (ECL) signal-amplified immunosensing strategy was proposed by using gold nanoparticles (Au NPs) functionalized reduced graphene oxide (rGO) capped Fe3O4(Au-FrGO). In this work, CeO2@TiO2was prepared by a sol-gel method to wrap CeO2with TiO2. In the presence of CeO2, CeO2@TiO2exhibited better ECL activity than TiO2with peroxydisulfate as coreactant. In addition, FrGO with macroporous structure was synthesized by self-assembly of rGO sheets capped cationic Fe3O4nanoparticles, exhibiting larger specific surface area than rGO. Due to the low toxicity and magnetism of Fe3O4, FrGO owned more favorable biocompatibility and the application of magnetic-separation simplified the preparation procedure. After hybridizing with Au NPs, FrGO exhibited more excellent electrical conductivity and could immobilize more CeO2@TiO2and antibodies. Therefore, a novel label-free ECL immunosensor based on Au-FrGO-CeO2@TiO2was constructed which generated higher ECL response. To investigate the performance of the immunosensor, carcinoembryonic antigen (CEA) was chosen as a model target analyte. Under optimal conditions, the immunosensor had sensitive response to CEA in a wide linear range of 0.01pgmL-1to 10ngmL-1with a detection limit of 3.28 fg mL-1. The proposed ECL immunosensor exhibited excellent stability, repeatability and selectivity, which opened another promising avenue for CEA determination in real serum samples.
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Lei
L
Yang
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Wenjuan
W
Zhu
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Xiang
X
Ren
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Malik Saddam
MS
Khan
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Yong
Y
Zhang
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Bin
B
Du
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China. Electronic address: sdjndxwq@163.com.
Qin
Q
Wei
D015374:Biosensing Techniques / Q000379:methods; D002272:Carcinoembryonic Antigen / Q000097:blood*; D002563:Cerium / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D016062:Porosity; D014025:Titanium / Q000737:chemistry*
D002272:Carcinoembryonic Antigen; C009495:titanium dioxide; D002563:Cerium; C030583:ceric oxide; D006046:Gold; D006108:Graphite; D014025:Titanium; D052203:Ferrosoferric Oxide
Carcinoembryonic antigen; CeO(2)@TiO(2); Electrochemiluminescence; Graphene capped Fe(3)O(4); Immunosensor