Alleviation of drought stress by mycorrhizas is related to increased root H2O2efflux in trifoliate orange.
The Non-invasive Micro-test Technique (NMT) is used to measure dynamic changes of specific ions/molecules non-invasively, but information about hydrogen peroxide (H2O2) fluxes in different classes of roots by mycorrhiza is scarce in terms of NMT. Effects of Funneliformis mosseae on plant growth, H2O2, superoxide radical (O2·-), malondialdehyde (MDA) concentrations, and H2O2fluxes in the taproot (TR) and lateral roots (LRs) of trifoliate orange seedlings under well-watered (WW) and drought stress (DS) conditions were studied. DS strongly inhibited mycorrhizal colonization in the TR and LRs, whereas mycorrhizal inoculation significantly promoted plant growth and biomass production. H2O2, O2·-, and MDA concentrations in leaves and roots were dramatically lower in mycorrhizal seedlings than in non-mycorrhizal seedlings under DS. Compared with non-mycorrhizal seedlings, mycorrhizal seedlings had relatively higher net root H2O2effluxes in the TR and LRs especially under WW, as well as significantly higher total root H2O2effluxes in the TR and LRs under WW and DS. Total root H2O2effluxes were significantly positively correlated with root colonization but negatively with root H2O2and MDA concentrations. It suggested that mycorrhizas induces more H2O2effluxes of the TR and LRs, thus, alleviating oxidative damage of DS in the host plant.
College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei 434025, China.|Institute of Root Biology, Yangtze University, Jingzhou, Hubei 434025, China.|Institute of Fruit and Tea, Hubei Academy of Agricultural Sciences, Wuhan, Hubei 430064, China.
Yong-Ming
YM
Huang
College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei 434025, China.|Institute of Root Biology, Yangtze University, Jingzhou, Hubei 434025, China.
Ying-Ning
YN
Zou
College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei 434025, China.|Institute of Root Biology, Yangtze University, Jingzhou, Hubei 434025, China.|Department of Chemistry, Faculty of Science, University of Hradec Kralove, Hradec Kralove 50003, Czech Republic.
Qiang-Sheng
QS
Wu
D018533:Biomass; D015169:Colony Count, Microbial; D055864:Droughts; D006861:Hydrogen Peroxide / Q000378:metabolism*; D016014:Linear Models; D008315:Malondialdehyde / Q000378:metabolism; D038821:Mycorrhizae / Q000254:growth & development / Q000502:physiology*; D032104:Poncirus / Q000382:microbiology* / Q000502:physiology*; D013312:Stress, Physiological; D013481:Superoxides / Q000378:metabolism; D014867:Water / Q000378:metabolism
D014867:Water; D013481:Superoxides; D008315:Malondialdehyde; D006861:Hydrogen Peroxide