Exosome secretome and mediated signaling in breast cancer patients with nontuberculous mycobacterial disease.
Bronchiectasis Nontuberculous mycobacterium (NTMnb) infection is an emerging health problem in breast cancer (BCa) patients. We measured sera exosome proteome in BCa-NTMnb subjects and controls by Mass Spectroscopy. Extracellular matrix protein 1 (ECM1) was detected exclusively in the circulating exosomes of 82% of the BCa-NTMnb cases. Co-culture of ECM1+ exosomes with normal human mammary epithelial cells induced epithelial to mesenchymal transition accompanied by increased Vimentin/CDH1 expression ratio and Glutamate production. Co-culture of the ECM1+ exosomes with normal human T cells modulated their cytokine production. The ECM1+ exosomes were markedly higher in sera obtained from BCa-NTMnb subjects. Exclusive expression of APN, APOC4 and AZGP1 was evident in the circulating exosomes of these BCa-NTMnb cases, which predicts disease prevalence independent of the body max index in concert with ECM1. Monitoring ECM1, APN, APOC4 and AZGP1 in the circulating exosomes could be beneficial for risk assessment, monitoring and surveillance of BCa-NTMnb.
Department of Medicine, The University of Texas Health Science Center at Tyler, Texas, USA.
Julie V
JV
Philley
Cellular and Molecular Biology, The University of Texas Health Science Center at Tyler, Texas, USA.
Anbarasu
A
Kannan
Department of Medicine, The University of Texas Health Science Center at Tyler, Texas, USA.
David E
DE
Griffith
Department of Medicine, The University of Texas Health Science Center at Tyler, Texas, USA.
Megan S
MS
Devine
Department of Medicine, The University of Texas Health Science Center at Tyler, Texas, USA.
Jeana L
JL
Benwill
Department of Medicine, The University of Texas Health Science Center at Tyler, Texas, USA.|The Mycobacteria/Nocardia Research Laboratory Department of Microbiology, The University of Texas Health Science Center at Tyler, Texas, USA.
Richard J
RJ
Wallace
The Mycobacteria/Nocardia Research Laboratory Department of Microbiology, The University of Texas Health Science Center at Tyler, Texas, USA.
Barbara A
BA
Brown-Elliott
Department of Medicine, The University of Texas Health Science Center at Tyler, Texas, USA.
Foram
F
Thakkar
Department of Medicine, The University of Texas Health Science Center at Tyler, Texas, USA.
Varsha
V
Taskar
Department of Medicine, The University of Texas Health Science Center at Tyler, Texas, USA.
James G
JG
Fox
Department of Medicine, The University of Texas Health Science Center at Tyler, Texas, USA.
Ammar
A
Alqaid
Department of Medicine, The University of Texas Health Science Center at Tyler, Texas, USA.
Hernaina
H
Bains
Medical Oncology, Tata Memorial Center, Mumbai, India.
Sudeep
S
Gupta
Cellular and Molecular Biology, The University of Texas Health Science Center at Tyler, Texas, USA.
Santanu
S
Dasgupta
D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology*; D001987:Bronchiectasis / Q000150:complications / Q000382:microbiology; D018920:Coculture Techniques; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology; D055354:Exosomes / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D013058:Mass Spectrometry; D008875:Middle Aged; D009164:Mycobacterium Infections / Q000150:complications*; D015398:Signal Transduction / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology; D059467:Transcriptome
C490607:ECM1 protein, human; D016326:Extracellular Matrix Proteins
ECM1; biomarker; breast cancer; exosomes; nontuberculous Mycobacterium