A combined approach of hollow microneedles and nanocarriers for skin immunization with plasmid DNA encoding ovalbumin.
The aim of this study was to investigate the use of different types of microneedles (MNs) and nanocarriers for in vitro skin permeation and in vivo immunization of plasmid DNA encoding ovalbumin (pOVA). In vitro skin permeation studies indicated that hollow MNs had a superior enhancing effect on skin permeation compared with solid MN patches, electroporation (EP) patches, the combination of MN and EP patches, and untreated skin. Upon using hollow MNs combined with nanocarriers for pOVA delivery, the skin permeation was higher than for the delivery of naked pOVA, as evidenced by the increased amount of pOVA in Franz diffusion cells and immunoglobulin G (IgG) antibody responses. When the hollow MNs were used for the delivery of nanocarrier:pOVA complexes into the skin of mice, they induced a stronger IgG immune response than conventional subcutaneous (SC) injections. In addition, immunization of mice with the hollow MNs did not induce signs of skin infection or pinpoint bleeding. Accordingly, the hollow MNs combined with a nanocarrier delivery system is a promising approach for delivering pOVA complexes to the skin for promoting successful immunization.
Department of Pharmaceutical Technology, Faculty of Pharmacy, Pharmaceutical Development of Green Innovations Group, Silpakorn University, Nakhon Pathom.
Boonnada
B
Pamornpathomkul
Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok.
Adisak
A
Wongkajornsilp
Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.
Wanida
W
Laiwattanapaisal
Department of Pharmaceutical Technology, Faculty of Pharmacy, Pharmaceutical Development of Green Innovations Group, Silpakorn University, Nakhon Pathom.
Theerasak
T
Rojanarata
Department of Pharmaceutical Technology, Faculty of Pharmacy, Pharmaceutical Development of Green Innovations Group, Silpakorn University, Nakhon Pathom.
Praneet
P
Opanasopit
Department of Pharmaceutical Technology, Faculty of Pharmacy, Pharmaceutical Development of Green Innovations Group, Silpakorn University, Nakhon Pathom.
Tanasait
T
Ngawhirunpat
D000279:Administration, Cutaneous; D000818:Animals; D000917:Antibody Formation; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000008:administration & dosage; D016503:Drug Delivery Systems; D018274:Electroporation; D005260:Female; D006367:HeLa Cells; D006801:Humans; D007114:Immunization / Q000295:instrumentation*; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D009339:Needles; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology*; D010957:Plasmids / Q000008:administration & dosage*; D011108:Polymers / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000276:immunology / Q000378:metabolism*
D007074:Immunoglobulin G; D011108:Polymers; D010047:Ovalbumin; D004247:DNA
electroporation; hollow microneedle; nanocarrier; plasmid DNA encoding ovalbumin; skin immunization; solid microneedle