Effects of Angelicae dahuricae Radix on 2, 4-Dinitrochlorobenzene-Induced Atopic Dermatitis-Like Skin Lesions in mice model.
BACKGROUND
Atopic dermatitis (AD) is an inflammatory, chronically relapsing, and intensively pruritic skin disease that affect 10-30% of the global population. Angelicae dahuricae Radix (ADR) has been reported to be anti-inflammatory in Korean Medicine. In the present study, we investigated whether ADR suppresses the progression of AD in animal model.


METHODS
AD was induced by 2, 4-Dinitrochlorobenzene (DNCB). ADR was orally administered to mice to study the effect of ADR on AD. Histological Analysis, immunohistochemistry, blood analysis, RT-PCR, and ELISA assay were performed.


RESULTS
ADR significantly suppressed AD-like symptoms in BALB/c mice: ADR decreased skin thickness and spleen weight of mice. ADR reduced infiltration of mast cells, inflammatory cells and CD4+ cells into mouse skin. ADR lowered the number of WBCs in the blood of mice. ADR reduced the levels of IgE, IL-6, IL-10 and IL-12 in mice serum. ADR down-regulated mRNA expression of IL-4, IL-6 and TNF-α in mouse skin tissue.


CONCLUSIONS
Our present study clearly indicates that ADR suppresses the progression of AD induced by DNCB in BALB/c mice. This suggests that ADR might be a useful drug for the treatment of AD.
Department of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul, 02447, Korea.
Jin Mo
JM
Ku
Department of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul, 02447, Korea.
Se Hyang
SH
Hong
Department of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul, 02447, Korea.
Hyo In
HI
Kim
Department of Preventive Medicine, College of Korean Medicine, Kyung Hee University, 1 Hoegi, Seoul, 130-701, Korea.
Hye Sook
HS
Seo
Department of Preventive Medicine, College of Korean Medicine, Kyung Hee University, 1 Hoegi, Seoul, 130-701, Korea.
Yong Cheol
YC
Shin
Department of Preventive Medicine, College of Korean Medicine, Kyung Hee University, 1 Hoegi, Seoul, 130-701, Korea. epiko@khu.ac.kr.
Seong-Gyu
http://orcid.org/0000-0001-9260-8889
SG
Ko
D000279:Administration, Cutaneous; D029969:Angelica; D000818:Animals; D018926:Anti-Allergic Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D003876:Dermatitis, Atopic / Q000188:drug therapy* / Q000276:immunology; D004137:Dinitrochlorobenzene; D004195:Disease Models, Animal; D008297:Male; D008519:Medicine, Traditional; D051379:Mice; D008807:Mice, Inbred BALB C; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000627:therapeutic use*; D056910:Republic of Korea; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
D018926:Anti-Allergic Agents; D004137:Dinitrochlorobenzene; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha
2, 4-Dinitrocholrlbenzene; Angelicae dahuricae Radix; Atopic dermatitis; BALB/c mice; Cytokine; Inflammation