An electrochemiluminescence biosensor for Kras mutations based on locked nucleic acid functionalized DNA walkers and hyperbranched rolling circle amplification.
Herein, an electrochemiluminescence (ECL) biosensor for ultrasensitive and specific detection of Kras mutant genes has been developed on the basis of the high discrimination capability of locked nucleic acid (LNA) and dual signal amplification techniques including DNA walkers and hyperbranched rolling circle amplification (HRCA).
Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350116, China.
Ying
Y
Zhang
Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350116, China.
Lixu
L
Wang
College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian 350116, China. luofang0812@163.com wengzq@fzu.edu.cn.
Fang
http://orcid.org/0000-0003-1480-0677
F
Luo
Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350116, China.
Bin
B
Qiu
Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350116, China.
Longhua
http://orcid.org/0000-0003-0706-0973
L
Guo
College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian 350116, China. luofang0812@163.com wengzq@fzu.edu.cn.
Zuquan
Z
Weng
Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350116, China.
Zhenyu
Z
Lin
Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350116, China.
Guonan
G
Chen
D015374:Biosensing Techniques; D004247:DNA / Q000235:genetics*; D004261:DNA Replication; D055664:Electrochemical Techniques; D006801:Humans; D008163:Luminescent Measurements; D009154:Mutation; D021141:Nucleic Acid Amplification Techniques; D009841:Oligonucleotides / Q000235:genetics*; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics*
C117307:KRAS protein, human; D009841:Oligonucleotides; C477371:locked nucleic acid; D004247:DNA; D016283:Proto-Oncogene Proteins p21(ras)