Plant growth promotion properties of bacterial strains isolated from the rhizosphere of the Jerusalem artichoke (Helianthus tuberosus L.) adapted to saline-alkaline soils and their effect on wheat growth.
The Jerusalem artichoke (JA; Helianthus tuberosus), known to be tolerant to saline-alkaline soil conditions, has been cultivated for many years in the Yellow River delta, Shandong Province coastal zone, in China. The aim of our study was to isolate nitrogen-fixing bacteria colonizing the rhizosphere of JA and to characterize other plant growth promotion properties. The ultimate goal was to identify isolates that could be used as inoculants benefiting an economic crop, in particular for improving wheat growth production in the Yellow River delta. Bacterial strains were isolated from the rhizosphere soil of JA on the basis of growth on nitrogen-free Ashby medium. Identification and phylogenetic analysis was performed after nucleotide sequencing of 16S rRNA gene. Plant-growth-promoting traits, such as nitrogen fixation activity, phosphate solubilization activity, indole-3-acetic acid production, were determined using conventional methods. Eleven strains were isolated and 6 of them were further examined for their level of salt tolerance and their effect on plant growth promotion. Inoculation of Enterobacter sp. strain N10 on JA and wheat led to significant increases in both root and shoot dry mass and shoot height. Enterobacter sp. strain N10 appeared to be the best plant-growth-promoting rhizobacteria to increase wheat productivity in future field applications.
a Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, People's Republic of China.|b College of Earth Sciences, University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
Xiaolin
X
Liu
a Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, People's Republic of China.|c Marine Environmental Monitoring Station, Ocean and Fisheries Bureau of Binzhou City, Binzhou 256600, People's Republic of China.
Xiangyue
X
Li
a Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, People's Republic of China.
Yan
Y
Li
d Shanxi Agricultural University, Taigu 030801, People's Republic of China.
Runzhi
R
Li
a Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, People's Republic of China.
Zhihong
Z
Xie
D001419:Bacteria / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D002681:China; D006368:Helianthus / Q000382:microbiology*; D007210:Indoleacetic Acids / Q000378:metabolism; D009584:Nitrogen; D009586:Nitrogen Fixation; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D055049:Salt Tolerance; D012965:Sodium Chloride; D012988:Soil Microbiology; D014908:Triticum / Q000235:genetics / Q000254:growth & development*
D007210:Indoleacetic Acids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; C030737:indoleacetic acid; D009584:Nitrogen
Enterobacter; Helianthus tuberosus; plant growth test; plant-growth-promoting rhizobacteria (PGPR); rhizobactéries favorisant la croissance végétale (RFCV); test de croissance végétale