SIN-Dependent Dissociation of the SAD Kinase Cdr2 from the Cell Cortex Resets the Division Plane.
Proper division plane positioning is crucial for faithful chromosome segregation but also influences cell size, position, or fate [1]. In fission yeast, medial division is controlled through negative signaling by the cell tips during interphase and positive signaling by the centrally placed nucleus at mitotic entry [2-4]: the cell geometry network (CGN), controlled by the inhibitory cortical gradient of the DYRK kinase Pom1 emanating from the cell tips, first promotes the medial localization of cytokinetic ring precursors organized by the SAD kinase Cdr2 to pre-define the division plane [5-8]; then, massive nuclear export of the anillin-like protein Mid1 at mitosis entry confirms or readjusts the division plane according to nuclear position and triggers the assembly of a medial contractile ring [5, 9-11]. Strikingly, the Hippo-like septation initiation network (SIN) induces Cdr2 dissociation from cytokinetic precursors at this stage [12-14]. We show here that SIN-dependent phosphorylation of Cdr2 promotes its interaction with the 14-3-3 protein Rad24 that sequesters it in the cytoplasm during cell division. If this interaction is compromised, cytokinetic precursors are asymmetrically distributed in the cortex of newborn cells, leading to asymmetrical division if nuclear signaling is abolished. We conclude that, through this new function, the SIN resets the division plane in newborn cells to ensure medial division.
Institut Curie, PSL Research University, CNRS, UMR 144, 75005 Paris, France. Electronic address: sergio.rincon@curie.fr.
Sergio A
SA
Rincon
Institute of Genetics and Development of Rennes, CNRS, UMR 6290, 35043 Rennes Cedex, France.
Miguel
M
Estravis
Laboratoire de Spectrométrie de Masse Protéomique, Institut Curie, PSL Research University, 75005 Paris, France.
Florent
F
Dingli
Laboratoire de Spectrométrie de Masse Protéomique, Institut Curie, PSL Research University, 75005 Paris, France.
Damarys
D
Loew
Institut Curie, PSL Research University, CNRS, UMR 144, 75005 Paris, France; Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.
Phong T
PT
Tran
Institut Curie, PSL Research University, CNRS, UMR 144, 75005 Paris, France. Electronic address: anne.paoletti@curie.fr.
Anne
A
Paoletti
D059447:Cell Cycle Checkpoints; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D048749:Cytokinesis; D003593:Cytoplasm / Q000502:physiology*; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics / Q000502:physiology*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction
D018797:Cell Cycle Proteins; D047908:Intracellular Signaling Peptides and Proteins; D029702:Schizosaccharomyces pombe Proteins; C501114:rad24 protein, S pombe; C542567:Cdr2 protein, S pombe; D017346:Protein Serine-Threonine Kinases
14-3-3 proteins; SIN pathway; cytokinesis; division plane; fission yeast