Rapid diagnosis of Vibrio owensii responsible for shrimp acute hepatopancreatic necrosis disease with isothermal recombinase polymerase amplification assay.
A rapid and sensitive AHPND-RPA assay was developed for the specific detection of the AHPND-causing Vibrio owensii. The AHPND-RPA detected as few as 2 copies per reaction in 9.02 ± 0.66 min at 39 °C, and showed 100% positive predictive value and negative predictive value for AHPND diagnosis.
College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
Liyuan
L
Liu
College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
Luzhi
L
Jiang
College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
Yongxin
Y
Yu
College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
Xiaoming
X
Xia
College of Food Science and Technology, Shanghai Ocean University, Shanghai, China; Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, China.
Yingjie
Y
Pan
College of Food Science and Technology, Shanghai Ocean University, Shanghai, China; Institute of Biochemistry and Molecular Cell Biology, University of Göttingen, Göttingen, Germany.
Shuling
S
Yan
College of Food Science and Technology, Shanghai Ocean University, Shanghai, China; Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, China. Electronic address: yjwang@shou.edu.cn.
Yongjie
Y
Wang
D000820:Animal Diseases / Q000175:diagnosis* / Q000235:genetics / Q000382:microbiology; D000818:Animals; D043143:Hepatopancreas / Q000382:microbiology / Q000473:pathology; D054884:Host-Pathogen Interactions / Q000235:genetics; D017747:Seafood / Q000382:microbiology*; D014733:Vibrio / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity
Acute hepatopancreatic necrosis disease; Diagnosis; RPA; Recombinase polymerase amplification; Vibrio owensii