The Analysis of Physiological Variations in M2Generation ofSolanum melongenaL. Mutagenized by Ethyl Methane Sulfonate.
The eggplant was mutagenized with ethyl methane sulfonate (EMS) to enhance its genetic variability in our previous paper. In this article, we further analyzed the phenotype of M2generation of mutant eggplants. A total of 325 independent M2families were investigated for phenotypic variation. In addition to the visible phenotypic variation, chlorogenic acid (CGA) concentrations were analyzed in 26 fruits of mutants with High Performance Liquid Chromatography assay. Seventeen fruits exhibited significantly higher concentrations of CGAs than those in wild-type. The anthocyanin concentration of S9-1, the purple black mutant, was higher than WT, meanwhile, the anthocyanin concentration of L6-4 and U36-1 was lower than WT. Furthermore, our RT-PCR result demonstrated that the expression levels of anthocyanin biosynthetic genes, except for SmPAL, were increased in S9-1, and the regulatorSmMYB1was decreased in L6-4 and U36-1 mutants. Together, our data indicated that, M2generation showed abundant phenotypic variations and the strong potential usage for next step of breeding and molecular genetic mechanisms in eggplant.
South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS)Zhanjiang, China; Zhanjiang City Key Laboratory for Tropical Crops Genetic ImprovementZhanjiang, China.
Xiao
X
Xi-Ou
South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS)Zhanjiang, China; Zhanjiang City Key Laboratory for Tropical Crops Genetic ImprovementZhanjiang, China.
Lin
L
Wenqiu
South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS)Zhanjiang, China; Zhanjiang City Key Laboratory for Tropical Crops Genetic ImprovementZhanjiang, China.
Li
L
Wei
South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS)Zhanjiang, China; Zhanjiang City Key Laboratory for Tropical Crops Genetic ImprovementZhanjiang, China.
Gao
G
Xiaoming
South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS)Zhanjiang, China; Zhanjiang City Key Laboratory for Tropical Crops Genetic ImprovementZhanjiang, China.
Lv
L
Lingling
South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS)Zhanjiang, China; Zhanjiang City Key Laboratory for Tropical Crops Genetic ImprovementZhanjiang, China.
Ma
M
Feiyue
South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS)Zhanjiang, China; Zhanjiang City Key Laboratory for Tropical Crops Genetic ImprovementZhanjiang, China.
Liu
L
Yuge
EMS mutant; M2 generation; anthocyanin; chlorogenic acid; eggplant; phenotypic variation