The bovine colostrum microbiome and its association with clinical mastitis.
In an effort to characterize colostrum microbial diversity and its potential associations with early-lactation clinical mastitis, we used high-throughput sequencing of the 16S rRNA gene to investigate the bovine colostrum microbiome. A prospective observational study was conducted that included 70 Holstein cows; colostrum samples were collected from all 4 mammary gland quarters. Colostrum samples were categorized according to whether the quarter was diagnosed (CMC) or not diagnosed (NCMC) with clinical mastitis during the first 30 d postpartum. Colostrum samples were dominated by Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, Fusobacteria, and Tenericutes phyla, with the 6 most common taxa [order (o), family (f), and genus (g)] being g_Staphylococcus, g_Prevotella, f_Ruminococcaceae, o_Bacteroidales, o_Clostridiales, and g_Pseudomonas. The colostrum microbiota of primiparous cows was significantly richer (higher number of bacterial species) than that of multiparous cows, and differences in colostrum taxonomic structure between parities were also observed. The microbial community of NCMC samples of primiparous cows was significantly more diverse than that of CMC samples, and the relative abundances of the Tenericutes and Fusobacteria phyla as well as the Mycoplasma and Fusobacterium genera were significantly higher in NCMC than in CMC samples of primiparous cows. The colostrum core microbiome, defined as the bacterial taxa common to all colostrum samples examined, was composed of 20 taxa and included bacterial genera already known to be associated with mastitis (e.g., Staphylococcus, Mycoplasma, and Streptococcus spp.). Our results indicate that the colostrum microbiome of primiparous cows differs from that of multiparous cows, and it harbors some diversity and taxonomic markers of mammary gland health specific to primiparous cows only.
Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca 14853, New York.
Svetlana F
SF
Lima
Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca 14853, New York.
Andre G V
AGV
Teixeira
Department of Veterinary Medical Science, College of Veterinary Medicine, University of Illinois, Urbana 61802.
Fabio S
FS
Lima
Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca 14853, New York.
Erika K
EK
Ganda
Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca 14853, New York.
Catherine H
CH
Higgins
Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca 14853, New York; Department of Epidemiology and Population Health, Institute of Infection and Global Health, University of Liverpool, Neston CH64 7TE, United Kingdom.
Georgios
G
Oikonomou
Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca 14853, New York. Electronic address: rcb28@cornell.edu.
Rodrigo C
RC
Bicalho
D000818:Animals; D002417:Cattle; D003126:Colostrum; D005260:Female; D007774:Lactation; D008414:Mastitis, Bovine / Q000382:microbiology; D064307:Microbiota; D011446:Prospective Studies; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
D012336:RNA, Ribosomal, 16S
colostrum; mastitis; microbiome