Cytological characterization of an interspecific hybrid inJatrophaand its progeny reveals preferential uniparental chromosome transmission and interspecific translocation.
Genetic variation inJatropha curcas, a prospective biodiesel plant, is limited, and interspecific hybridization needed for its genetic improvement. Progeny from interspecific crosses betweenJ. curcasandJatropha integerrimacan be used to improve agronomic characters and to increase oil content and yield. However, these hybrids have not been characterized cytologically. The present study was aimed at the analysis of chromosome behavior during meiosis and chromosome composition of S1plants derived from an interspecific F1hybrid using genomicin situhybridization (GISH) and fluorescencein situhybridization (FISH). Bivalents that formed as a result of interspecific pairing were frequently observed, suggesting the presence of homoeologous chromosomes from the two species. Almost half of microspores were derived from the reduction division; GISH analysis indicated random transmission of the parent chromosomes to microspores. Male fertility measured as pollen staining with acetocarmine was 48.4%. In contrast, GISH analysis of S1plants revealed preferential transmission ofJ. curcaschromosomes. We also found segment exchange between chromosomes of the two species (interspecific translocation) by GISH and FISH analyses. Introgression ofJ. integerrimachromosome segments into theJ. curcasgenome would help to improveJatrophacultivars for mass production.
Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University , Matsudo 648, Chiba 271-8510 , Japan.
Shuto
S
Fukuhara
Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University, Matsudo 648, Chiba 271-8510, Japan; Faculty of Agriculture, Princess Naradhiwas University, Narathiwat 96110, Thailand.
Narathid
N
Muakrong
Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University , Matsudo 648, Chiba 271-8510 , Japan.
Shinji
S
Kikuchi
Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand; Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok 10900 Thailand (CASAF, NRU-KU, Thailand).
Patcharin
P
Tanya
Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University , Matsudo 648, Chiba 271-8510 , Japan.
Hidenori
H
Sassa
Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University , Matsudo 648, Chiba 271-8510 , Japan.
Takato
T
Koba
Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand; Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok 10900 Thailand (CASAF, NRU-KU, Thailand).
Peerasak
P
Srinives
Jatropha curcas; Jatropha integerrima; fluorescence in situ hybridization (FISH); genomic in situ hybridization (GISH); interspecific hybrid; interspecific translocation; preferential transmission