Internalization kinetics of the gonadotropin-releasing hormone (GnRH) receptor.
This study quantified the agonist-induced endocytotic and recycling events of the mammalian gonadotropin releasing hormone receptor (GnRH-R) and investigated the role of the intracellular carboxyl (C)-terminal tail in regulating agonist-induced receptor internalization kinetics. The rate of internalization for the rat GnRH-R was found to be exceptionally low when compared with G-protein coupled receptors (GPCRs) which possess a cytoplasmic C-terminal tail (thyrotropin-releasing hormone receptor (TRH-R), catfish GnRH-R (cfGnRH-R) and GnRH/TRH-R chimeric receptor). These data provide evidence that the presence of a functional intracellular cytoplasmic C-terminal tail is essential for rapid internalization of the studied GPCRs.
KA Eidne; MRC Reproductive Biology Unit, 37 Chalmers Street EH3 9EW Edinburgh, United Kingdom, , , , , , GB.
Milka
M
Vrecl
KA Eidne; MRC Reproductive Biology Unit, 37 Chalmers Street EH3 9EW Edinburgh, United Kingdom, , , , , , GB.
Anders
A
Heding
KA Eidne; MRC Reproductive Biology Unit, 37 Chalmers Street EH3 9EW Edinburgh, United Kingdom, , , , , , GB.
Aylin
A
Hanyaloglu
KA Eidne; MRC Reproductive Biology Unit, 37 Chalmers Street EH3 9EW Edinburgh, United Kingdom, , , , , , GB.
Philip L
PL
Taylor
KA Eidne; MRC Reproductive Biology Unit, 37 Chalmers Street EH3 9EW Edinburgh, United Kingdom, , , , , , GB.
Karin A
KA
Eidne
G-protein coupled receptor; Key words, GnRH receptor; endocytosis; internalization kinetics; recycling