The rat vomeronasal organ is a vitamin D target.
We studied the expression of vitamin D receptor and of vitamin D binding protein in the rat vomeronasal organ. With immunofluorescence, in situ hybridization and with reverse transcriptase PCR we found both proteins in sensory as well as in non-sensory cells. Sensory neurons contained immunoreactivity for vitamin D3 receptor in nuclei, in portions of the cytoplasm, and in apical dendrites and their microvilli. Vitamin D binding protein was observed in sensory neuron axons and cytoplasm, mostly confined to dendrites. Colocalization appeared in the contact zone of supporting cells and sensory dendrites. Both proteins were also found in single ciliated cells within the non-sensory epithelium. Vitamin D binding protein was also localized in secretory vesicles in a portion of the vomeronasal glands. Our findings suggest that the rat vomeronasal organ is a vitamin D target.
Institute of Anatomy II, University Hospital, Friedrich Schiller University, Jena, Germany. Electronic address: Andrea.Rodewald@med.uni-jena.de.
A
A
Rodewald
Institute of Anatomy II, University Hospital, Friedrich Schiller University, Jena, Germany.
V M
VM
Gebhart
Institute of Anatomy II, University Hospital, Friedrich Schiller University, Jena, Germany.
H
H
Oehring
Institute of Anatomy II, University Hospital, Friedrich Schiller University, Jena, Germany.
G F
GF
Jirikowski
D000818:Animals; D016503:Drug Delivery Systems / Q000379:methods; D005260:Female; D008297:Male; D009831:Olfactory Mucosa / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018167:Receptors, Calcitriol / Q000032:analysis / Q000378:metabolism*; D011984:Sensory Receptor Cells / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D014807:Vitamin D / Q000008:administration & dosage / Q000378:metabolism*; D014809:Vitamin D-Binding Protein / Q000032:analysis / Q000378:metabolism*; D019147:Vomeronasal Organ / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*
D018167:Receptors, Calcitriol; D014809:Vitamin D-Binding Protein; D014807:Vitamin D
Immunohistochemistry; RT-PCR; Rat vomeronasal organ; Sensory cells; Vitamin D binding protein; Vitamin D receptor