Terbinafine-loaded wound dressing for chronic superficial fungal infections.
In spite of developing new drugs and modern formulations, the treatments of chronic fungal infections are still challenging. Fibrous wound dressings are new suggestions for the treatment of chronic superficial infections. In the present study, we formulated an antifungal agent, terbinafine hydrochloride (TFH), which is a hydrophobic drug, in wound dressings prepared by electrospun polycaprolactone, polycaprolactone/gelatin (50:50 w/w) and gelatin. To obtain more water-stable meshes, the preparations were treated by glutaraldehyde and their properties were determined before and after treatment. The morphology of fibrous meshes was observed by scanning electron microscopy. Drug loading efficiency and release rate were measured by high performance liquid chromatography (HPLC) and the release rate was monitored for 144h. Antifungal tests were performed on Trichophyton mentagrophytes, Aspergillus fumigatus and Candida albicans cultured on Muller-Hinton agar. The toxicity of the meshes was measured after 24h and 14days by MTT assay. Terbinafine loading of polycaprolactone/gelatin (50:50) was 100% and it released the highest amount of TFH too. In antifungal tests, all samples were able to hinderT. mentagrophytes and A. fumigatus but not C. albicans growth among them, polycaprolactone fibers made the largest inhibition zone. In MTT assay, none of prepared samples showed toxicity against L929 cells. Teken together, the prepared TFH-loaded PCL/gelatin electrospun meshes were able to release TFH slowly and in a steady state in time. With respect to no obvious cytotoxicity in MTT assay and stong antifungal activity toward T. mentagrophytesin vitro, these TFH-based meshes could be considered as potential candidates in clinical application as wound dressing for treatment of chronic dermatophytosis.
Department of Mycology, Pasteur Institute of Iran, Tehran 13164. Iran; Microbiology Research Center, Pasteur Institute of Iran, Tehran 13164. Iran.
Farnoush Asghari
FA
Paskiabi
National Cell Bank Department, Pasteur Institute of Iran, Tehran 13164, Iran.
Shahin
S
Bonakdar
National Cell Bank Department, Pasteur Institute of Iran, Tehran 13164, Iran.
Mohammad Ali
MA
Shokrgozar
Department of Novel Drug Delivery Systems, Iran Polymer and Petrochemical Institute, Tehran, Iran.
Mohammad
M
Imani
Department of Mycology, Pasteur Institute of Iran, Tehran 13164. Iran.
Zahra
Z
Jahanshiri
Department of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Masoomeh
M
Shams-Ghahfarokhi
Department of Mycology, Pasteur Institute of Iran, Tehran 13164. Iran; Microbiology Research Center, Pasteur Institute of Iran, Tehran 13164. Iran. Electronic address: mrab442@yahoo.com.
Mehdi
M
Razzaghi-Abyaneh
D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001458:Bandages / Q000382:microbiology*; D002460:Cell Line; D002908:Chronic Disease; D065546:Drug Liberation; D005780:Gelatin / Q000737:chemistry; D051379:Mice; D009181:Mycoses / Q000188:drug therapy* / Q000382:microbiology; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D009281:Naphthalenes / Q000494:pharmacology / Q000627:therapeutic use*; D011091:Polyesters / Q000737:chemistry; D000077291:Terbinafine; D014947:Wounds and Injuries / Q000188:drug therapy* / Q000382:microbiology*
D000935:Antifungal Agents; D009281:Naphthalenes; D011091:Polyesters; C016240:polycaprolactone; D005780:Gelatin; D000077291:Terbinafine
Dermatophytes; Gelatin; Polycaprolactone; Superficial fungal infections; Terbinafine hydrochloride; Wound dressing