Developing a new nonbinary SNP fluorescent multiplex detection system for forensic application in China.
Nonbinary single-nucleotide polymorphisms (SNPs) are potential forensic genetic markers because their discrimination power is greater than that of normal binary SNPs, and that they can detect highly degraded samples. We previously developed a nonbinary SNP multiplex typing assay. In this study, we selected additional 20 nonbinary SNPs from the NCBI SNP database and verified them through pyrosequencing. These 20 nonbinary SNPs were analyzed using the fluorescent-labeled SNaPshot multiplex SNP typing method. The allele frequencies and genetic parameters of these 20 nonbinary SNPs were determined among 314 unrelated individuals from Han populations from China. The total power of discrimination was 0.9999999999994, and the cumulative probability of exclusion was 0.9986. Moreover, the result of the combination of this 20 nonbinary SNP assay with the 20 nonbinary SNP assay we previously developed demonstrated that the cumulative probability of exclusion of the 40 nonbinary SNPs was 0.999991 and that no significant linkage disequilibrium was observed in all 40 nonbinary SNPs. Thus, we concluded that this new system consisting of new 20 nonbinary SNPs could provide highly informative polymorphic data which would be further used in forensic application and would serve as a potentially valuable supplement to forensic DNA analysis.
Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha, P.R. China.
Yanfang
Y
Liu
Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha, P.R. China.
Huidan
H
Liao
Department of Oral and Maxillofacial Surgery, Xiangya Stomatological Hospital, Central South University, Changsha, P.R. China.
Ying
Y
Liu
Department of Oral and Maxillofacial Surgery, Xiangya Stomatological Hospital, Central South University, Changsha, P.R. China.
Juanjuan
J
Guo
Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha, P.R. China.
Yi
Y
Sun
Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha, P.R. China.
Xiaoliang
X
Fu
Research Center of Carcinogenesis and Targeted Therapy, Xiangya Hospital, Central South University, Changsha, P.R. China.
Ding
D
Xiao
Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha, P.R. China.
Jifeng
J
Cai
Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha, P.R. China.
Lingmei
L
Lan
Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha, P.R. China.
Pingli
P
Xie
Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha, P.R. China.
Lagabaiyila
L
Zha
D000818:Animals; D002681:China; D004586:Electrophoresis / Q000379:methods; D005453:Fluorescence; D044707:Forensic Sciences / Q000379:methods*; D005787:Gene Frequency; D005819:Genetic Markers; D005828:Genetics, Population; D006801:Humans; D015810:Linkage Disequilibrium; D020641:Polymorphism, Single Nucleotide; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
D005819:Genetic Markers
F-statistic; Forensic genetics; Nonbinary single nucleotide polymorphisms; Pyrosequencing; SNaPshot