A Novel RNA-Binding Protein Involves ABA Signaling by Post-transcriptionally RepressingABI2.
TheStress Associated RNA-binding protein 1(SRP1) repressed by ABA, salt and cold encodes a C2C2-type zinc finger protein inArabidopsis. The knock-out mutation insrp1reduced the sensitivity of seed to ABA and salt stress during germination and post-germinative growth stages. In contrast,SRP1-overexpressing seedlings were more sensitive to ABA and salt compared to wild type plants. In the presence of ABA, the transcript levels of ABA signaling and germination-related genes includingABI3. ABI5. EM1andEM6were less induced insrp1compared to WT. Interestingly, expression ofABI2encoding a protein phosphatase 2C protein were significantly up-regulated insrp1mutants. Byin vitroanalysis, SRP1 was identified as a novel RNA-binding protein directly binding to 3'UTR ofABI2mRNA. Moreover, transient expression assay proved the function of SRP1 in reducing the activity of luciferase whose coding sequence was fused with theABI23'UTR. Together, it is suggested that SRP1 is involved in the ABA signaling by post-transcriptionally repressingABI2expression inArabidopsis.
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural UniversityNanjing, China; Institute of Industrial Crops, Jiangsu Academy of Agricultural ScienceNanjing, China.
Jianwen
J
Xu
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural UniversityNanjing, China; Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural UniversityNanjing, China.
Yihan
Y
Chen
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural UniversityNanjing, China; Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural UniversityNanjing, China.
Luofeng
L
Qian
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural UniversityNanjing, China; Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural UniversityNanjing, China.
Rong
R
Mu
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural UniversityNanjing, China; Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural UniversityNanjing, China.
Xi
X
Yuan
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural UniversityNanjing, China; Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural UniversityNanjing, China.
Huimin
H
Fang
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural UniversityNanjing, China; Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural UniversityNanjing, China.
Xi
X
Huang
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural UniversityNanjing, China; Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural UniversityNanjing, China.
Enshun
E
Xu
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural UniversityNanjing, China; Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural UniversityNanjing, China.
Hongsheng
H
Zhang
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural UniversityNanjing, China; Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural UniversityNanjing, China.
Ji
J
Huang
ABA signaling; ABI2; Arabidopsis thaliana; RNA-binding protein; abiotic stress; zinc finger protein