Preparation of hydroxyapatite/collagen injectable bone paste with an anti-washout property utilizing sodium alginate. Part 1: influences of excess supplementation of calcium compounds.
The anti-washout property, viscosity, and cytocompatibility to an osteoblastic cell line, MG-63, of anti-washout pastes were investigated. Mixing a hydroxyapatite/collagen bone-like nanocomposite (HAp/Col), an aqueous solution of sodium alginate (Na-Alg), which is a paste hardening and lubricant agent, and supplementation of calcium carbonate or calcium citrate (Ca-Cit) as a calcium resource for the hardening reaction realized an injectable bone paste. Adding Ca-Cit at a concentration greater than eight times the Ca2+ion concentration to Na-Alg improved the anti-washout property. Although the viscosity test indicated a gradual increase in the paste viscosity as the calcium compounds increased, pastes with excess supplementation of calcium compounds exhibited injectability through a syringe with a 1.8 mm inner diameter, realizing an injectable bone filler. Furthermore, the anti-washout pastes with Ca-Cit had almost the same cell proliferation rate as that of the HAp/Col dense body. Therefore, HAp/Col injectable anti-washout pastes composed of the HAp/Col, Na-Alg, and Ca-Cit are potential candidates for bioresorbable bone filler pastes.
Department of Applied Chemistry, Graduated School of Science and Technology, Meiji University, Kawasaki, 214-8571, Japan.
Taira
T
Sato
Bioceramics Group, National Institute for Materials Science, Tsukuba, 305-0044, Japan. KIKUCHI.Masanori@nims.go.jp.
Masanori
http://orcid.org/0000-0002-9451-8147
M
Kikuchi
Department of Applied Chemistry, Graduated School of Science and Technology, Meiji University, Kawasaki, 214-8571, Japan. mamorua@meiji.ac.jp.
Mamoru
M
Aizawa
D000464:Alginates / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D001843:Bone Cements; D001862:Bone Resorption; D018786:Bone Substitutes / Q000737:chemistry*; D001842:Bone and Bones / Q000378:metabolism; D002118:Calcium / Q000737:chemistry*; D002119:Calcium Carbonate / Q000737:chemistry; D019355:Calcium Citrate / Q000737:chemistry; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D007477:Ions; D008422:Materials Testing; D053761:Nanocomposites / Q000737:chemistry; D011208:Powders; D014783:Viscosity
D000464:Alginates; D001672:Biocompatible Materials; D001843:Bone Cements; D018786:Bone Substitutes; D006603:Hexuronic Acids; D007477:Ions; D011208:Powders; D020723:Glucuronic Acid; D003094:Collagen; D017886:Durapatite; D002119:Calcium Carbonate; D019355:Calcium Citrate; D002118:Calcium