Formation of novel hydrogel bio-anode by immobilization of biocatalyst in alginate/polyaniline/titanium-dioxide/graphite composites and its electrical performance.
A new bio-anode containing gel-entrapped bacteria in alginate/polyaniline/TiO2/graphite composites was constructed and electrically investigated. Alginate as dopant and template as well as entrapped gel was used for immobilization of microorganism cells. Increase of polyaniline concentration resulted an increase in the conductivity in gels. Addition of 0.01 and 0.02 g/mL polyaniline caused 6-fold and 10-fold higher conductivity, respectively. Furthermore, addition of 0.05 g/mL graphite powder caused 10-fold higher conductivity and 4-fold higher power density, respectively. The combination of polyaniline and graphite resulted 105-fold higher conductivity and 7-fold higher power-density output. Optimized concentrations of polyaniline and graphite powder were determined to be 0.02 g/mL and 0.05 g/mL, respectively. Modified hydrogel anode was successfully used in microbial fuel cell systems both in semi- and continuous operations modes. In semi-continuous mode, about 7.88 W/m3power density was obtained after 13 h of fermentation. The glucose consumption rate was calculated to be about 7 mg glucose/h/1.2·107CFU immobilized cells. Similar power density was observed in the continuous operation mode of the microbial fuel cell, and it was operated stably for more than 7 days. Our results are very promising for development of an improved microbial fuel cell with new type of bio-anode that have higher power density and can operate for long term.
Institute of Bioengineering and Process Engineering, Szent István University, Ménesi út 45, H-1118 Budapest, Hungary.
Attila
A
Szöllősi
Institute of Bioengineering and Process Engineering, Szent István University, Ménesi út 45, H-1118 Budapest, Hungary.
Ágoston
Á
Hoschke
Institute of Bioengineering and Process Engineering, Szent István University, Ménesi út 45, H-1118 Budapest, Hungary.
Judit M
JM
Rezessy-Szabó
Institute of Bioengineering and Process Engineering, Szent István University, Ménesi út 45, H-1118 Budapest, Hungary.
Erika
E
Bujna
Institute of Bioengineering and Process Engineering, Szent István University, Ménesi út 45, H-1118 Budapest, Hungary.
Szilárd
S
Kun
Institute of Bioengineering and Process Engineering, Szent István University, Ménesi út 45, H-1118 Budapest, Hungary. Electronic address: Nguyen.Duc.Quang@etk.szie.hu.
Quang D
QD
Nguyen
D000464:Alginates / Q000737:chemistry*; D000814:Aniline Compounds / Q000737:chemistry*; D001673:Biodegradation, Environmental; D001674:Bioelectric Energy Sources; D004553:Electric Conductivity; D004560:Electricity; D004563:Electrochemistry / Q000295:instrumentation; D004566:Electrodes; D020723:Glucuronic Acid / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006603:Hexuronic Acids / Q000737:chemistry; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*
D000464:Alginates; D000814:Aniline Compounds; D006603:Hexuronic Acids; C416807:polyaniline; C009495:titanium dioxide; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D006108:Graphite; D020723:Glucuronic Acid; D014025:Titanium
Alginate/polyaniline/titanium-dioxide/graphite; Bio-anode; Conductive hydrogel; Immobilized cells; Microbial fuel cell; Shewanella algae