Application of modified hollow fiber liquid phase microextraction in conjunction with chromatography-electron capture detection for quantification of acrylamide in waste water samples at ultra-trace levels.
Herein, a simple and sensitive method was successfully developed for the extraction and quantification of acrylamide in water samples. Initially, acrylamide was derivatized through a bromination process. Subsequently, a modified hollow-fiber liquid-phase microextraction was applied for the extraction of the brominated acrylamide from a 10-ml portion of an aqueous sample. Briefly, in this method, the derivatized acrylamide (2,3-dibromopropionamide) was extracted from the aqueous sample into a thin layer of an organic solvent sustained in pores of a porous hollow fiber. Then, it was back-extracted using a small volume of organic acceptor solution (acetonitril, 25μl) located inside the lumen of the hollow fiber followed by gas chromatography-electron capture detection (GC-ECD). The optimal conditions were examined for the extraction of the analyte such as: the organic solvent: dihexyl ether+10% tri-n-octyl phosphine oxide; stirring rate: 750rpm; no salt addition and 30min extraction time. These optimal extraction conditions allowed excellent enrichment factor values for the method. Enrichment factor, detection limit (S/N=3) and dynamic linear range of 60, 2ngL-1and 50-1000ngL-1to be determined for the analyte. The relative standard deviations (RSD%) representing precision of the method were in the range of 2.2-5.8 based on the average of three measurements. Accuracy of the method was tested by the relative recovery experiments on spiked samples, with results ranging from 93 to 108%. Finally, the method proved to be simple, rapid, and cost-effective for routine screen of acrylamide-contaminated highly-complicated untreated waste water samples.
Department of Chemistry, Payame Noor University, Tehran, Iran.
Hamid Reza
HR
Sobhi
Iranian Research and Development Center for Chemical Industries, ACECR, Tehran, Iran.
Mahnaz
M
Ghambarian
Research Center for Environmental Determinants of Health (RCEDH), Kermanshah University of Medical Sciences, Kermanshah, Iran.
Mohammad
M
Behbahani
Research Center for Environmental Health Technology, Iran University of Medical Sciences, Iran; Department of Environmental Health Engineering, School of Public Health, Iran University of Medical Sciences, Tehran, Iran. Electronic address: ali.esrafily@gmail.com.
Ali
A
Esrafili
D020106:Acrylamide / Q000032:analysis*; D002623:Chemistry Techniques, Analytical / Q000191:economics / Q000379:methods* / Q000592:standards; D002849:Chromatography, Gas; D059627:Liquid Phase Microextraction; D012997:Solvents / Q000737:chemistry; D062065:Wastewater / Q000737:chemistry*
D020106:Acrylamide; D012997:Solvents; D062065:Wastewater
Acrylamide; Derivatization; Gas chromatography–electron capture; Modified hollow-fiber liquid phase microextraction; Waste water samples