A Dual-Modality System for Both Multi-Color Ultrasound-Switchable Fluorescence and Ultrasound Imaging.
Simultaneous imaging of multiple targets (SIMT) in opaque biological tissues is an important goal for molecular imaging in the future. Multi-color fluorescence imaging in deep tissues is a promising technology to reach this goal. In this work, we developed a dual-modality imaging system by combining our recently developed ultrasound-switchable fluorescence (USF) imaging technology with the conventional ultrasound (US) B-mode imaging. This dual-modality system can simultaneously image tissue acoustic structure information and multi-color fluorophores in centimeter-deep tissue with comparable spatial resolutions. To conduct USF imaging on the same plane (i.e.,x-zplane) as US imaging, we adopted two 90°-crossed ultrasound transducers with an overlapped focal region, while the US transducer (the third one) was positioned at the center of these two USF transducers. Thus, the axial resolution of USF is close to the lateral resolution, which allows a point-by-point USF scanning on the same plane as the US imaging. Both multi-color USF and ultrasound imaging of a tissue phantom were demonstrated.
Ultrasound and Optical Imaging Laboratory, Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76010, USA. jayanth.kandukurijayanth@mavs.uta.edu.|Joint Biomedical Engineering Program, University of Texas at Arlington and University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75235, USA. jayanth.kandukurijayanth@mavs.uta.edu.
Jayanth
J
Kandukuri
Ultrasound and Optical Imaging Laboratory, Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76010, USA. shuai.yu@mavs.uta.edu.|Joint Biomedical Engineering Program, University of Texas at Arlington and University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75235, USA. shuai.yu@mavs.uta.edu.
Shuai
S
Yu
Ultrasound and Optical Imaging Laboratory, Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76010, USA. bingbing.cheng@mavs.uta.edu.|Joint Biomedical Engineering Program, University of Texas at Arlington and University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75235, USA. bingbing.cheng@mavs.uta.edu.
Bingbing
B
Cheng
Department of Chemistry, University of North Texas, 1155, Union Circle, #305070, Denton, TX 76203, USA. venu235@yahoo.com.
Venugopal
V
Bandi
Department of Chemistry, University of North Texas, 1155, Union Circle, #305070, Denton, TX 76203, USA. francis.dsouza@unt.edu.
Francis
F
D'Souza
Joint Biomedical Engineering Program, University of Texas at Arlington and University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75235, USA. knguyen@uta.edu.|Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76010, USA. knguyen@uta.edu.
Kytai T
KT
Nguyen
Joint Biomedical Engineering Program, University of Texas at Arlington and University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75235, USA. yihong@uta.edu.|Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76010, USA. yihong@uta.edu.
Yi
Y
Hong
Ultrasound and Optical Imaging Laboratory, Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76010, USA. baohong@uta.edu.|Joint Biomedical Engineering Program, University of Texas at Arlington and University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75235, USA. baohong@uta.edu.
Baohong
B
Yuan
D003287:Contrast Media; D006801:Humans; D007091:Image Processing, Computer-Assisted; D057054:Molecular Imaging / Q000295:instrumentation / Q000379:methods*; D061848:Optical Imaging / Q000295:instrumentation / Q000379:methods*; D014463:Ultrasonography / Q000295:instrumentation / Q000379:methods*
D003287:Contrast Media
deep-tissue fluorescence imaging; dual-modality imaging; multi-color fluorescence imaging; ultrasound imaging; ultrasound-switchable fluorescence imaging