Detecting Sonolysis of Polyethylene Glycol Upon Functionalizing Carbon Nanotubes.
Polyethylene glycol (PEG) and related polymers are often used in the solubilization and noncovalent functionalization of carbon nanomaterials by sonication. For example, carbon nanotubes are frequently sonicated with PEG-containing surfactants of the Pluronic®series or phospholipid-PEG polymers to noncovalently functionalize the nanotubes. However, PEG is very sensitive to degradation upon sonication and the degradation products can be toxic to mammalian cells and to organisms such as zebrafish embryos. It is therefore useful to have a simple and inexpensive method to determine the extent of potential PEG sonolysis, as described in this chapter. Intact PEG polymers and degraded fragments are resolved on sodium dodecyl sulfate polyacrylamide gels by electrophoresis and visualized by staining with barium iodine (BaI2). Digitized images of gels are acquired using a flatbed photo scanner and the intensities of BaI2-stained PEG bands are quantified using ImageJ software. Degradation of PEG polymers after sonication is readily detected by the reduction of band intensities in gels compared to those of non-sonicated, intact PEG polymers. In addition, the approach can be used to rapidly screen various sonication conditions to identify those that might minimize PEG degradation to acceptable levels.
Department of Biological Sciences, The University of Texas at Dallas, Richardson, TX, USA.|Department of Chemistry & Biochemistry, The University of Texas at Dallas, Richardson, TX, USA.
Ruhung
R
Wang
Department of Biological Sciences, The University of Texas at Dallas, Richardson, TX, USA.
Vasanth S
VS
Murali
Department of Biological Sciences, The University of Texas at Dallas, Richardson, TX, USA. draper@utdallas.edu.|Department of Chemistry & Biochemistry, The University of Texas at Dallas, Richardson, TX, USA. draper@utdallas.edu.|NanoTech Institute, The University of Texas at Dallas, 800 West Campbell Road, Richardson, TX, 75080, USA. draper@utdallas.edu.
Rockford
R
Draper
D004591:Electrophoresis, Polyacrylamide Gel; D037742:Nanotubes, Carbon / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D013010:Sonication; D013194:Staining and Labeling
D037742:Nanotubes, Carbon; D011092:Polyethylene Glycols
Carbon nanotubes; Functionalization; Nanotoxicity; Phospholipid-PEG; Pluronic®; Polyethylene glycol; SDS-PAGE; Sonication