Rhd and C/cE/e genotyping in Slovenian population.
The Rhesus (Rh) blood group system is, after ABO, clinically most important. Alloantibodies directed against Rh antigens are the major cause of a haemolytic disease of newborn (HDN) and of transfusion reactions. In search for novel methods for Rh genotyping we started to compare Rh genotypes identified from different tissues and Rh phenotypes. Genotypes determined from blood samples with PCR based RhD, C/c and E/e genotyping methods were compared with serologically identified phenotypes (N=32). With two exceptions the results of phenotyping and genotyping were in concordance. Two Rh serotypes from a Slovenian family that were unexpected according to the Mendelian laws were characterised genotypically. The two family members were suspected to have a chromosomal deletion on RH gene locus.
Blood Transfusion Centre of Slovenia, Šlajmerjeva 6, SI-1105 Ljubljana, Slovenia, , , , , , SI.
Ruth Rebeka
RR
Rupreht
Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Plesmanlaan 125, 1067 CX Amsterdam, The Netherlands, , , , , , NL.
Brigitte Henrica Wilhelmina
BH
Faas
Blood Transfusion Centre of Slovenia, Šlajmerjeva 6, SI-1105 Ljubljana, Slovenia, , , , , , SI.
Vesna
V
Bojanič
Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Plesmanlaan 125, 1067 CX Amsterdam, The Netherlands, , , , , , NL.
Petra Anna Maaskant van
PA
Wijk
Blood Transfusion Centre of Slovenia, Šlajmerjeva 6, SI-1105 Ljubljana, Slovenia, , , , , , SI.
Ljerka
L
Glonar
Blood Transfusion Centre of Slovenia, Šlajmerjeva 6, SI-1105 Ljubljana, Slovenia, , , , , , SI.
Irena
I
Bricl
Blood Transfusion Centre of Slovenia, Šlajmerjeva 6, SI-1105 Ljubljana, Slovenia, , , , , , SI.
Primož
P
Rožman
Blood Transfusion Centre of Slovenia, Šlajmerjeva 6, SI-1105 Ljubljana, Slovenia, , , , , , SI.
Vladka Čurin
VČ
Šerbec
Key words Rhesus (Rh); PCR DNA typing; RHCE; RHD; Slovenian population