Dual-Site Fluorescent Probe for Visualizing the Metabolism of Cys in Living Cells.
Fluorescent probes, as noninvasive tools for visualizing the metabolism of biomolecules, hold great potential to explore their physiological and pathological processes. For cysteine (Cys), however, none of the reported fluorescent probes could image the metabolic processes in living cells. To achieve this goal, we developed a coumarin derivative based on rational design of the dual recognition sites for Cys and its metabolite, SO2. The probe displayed distinct two channels with turn-on fluorescent emission toward Cys and SO2, which were successfully applied for imaging both A549 cells and zebrafish. Further, with reversible fluorescent responses toward Cys, the probe could image the enzymatic conversion of Cys to SO2in living A549 cells in a ratiometric manner. The present work reports the first probe to image the endogenous generated SO2without incubation of the SO2donors.
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Key Laboratory of Materials for Energy Conversion and Storage of Shanxi Province, Institute of Molecular Science, Shanxi University , Taiyuan 030006, China.
Yongkang
Y
Yue
Research Institute of Applied Chemistry, Shanxi University , Taiyuan 030006, China.
Fangjun
F
Huo
Department of Chemistry, Anhui University , Hefei 230601, China.
Peng
P
Ning
Research Institute of Applied Chemistry, Shanxi University , Taiyuan 030006, China.
Yongbin
Y
Zhang
Research Institute of Applied Chemistry, Shanxi University , Taiyuan 030006, China.
Jianbin
J
Chao
Department of Chemistry, Anhui University , Hefei 230601, China.
Xiangming
X
Meng
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Key Laboratory of Materials for Energy Conversion and Storage of Shanxi Province, Institute of Molecular Science, Shanxi University , Taiyuan 030006, China.
Caixia
0000-0001-5548-6333
C
Yin
D000818:Animals; D045744:Cell Line, Tumor; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D015394:Molecular Structure; D013056:Spectrophotometry, Ultraviolet; D013458:Sulfur Dioxide / Q000737:chemistry / Q000378:metabolism*; D015027:Zebrafish
D005456:Fluorescent Dyes; D013458:Sulfur Dioxide; D003545:Cysteine