Oxidative stress and NO generation in the rat pancreatitis induced by pancreatic duct ligation.
The interaction between nitric oxide (NO) and superoxides is critical in the development of an acute pancreatitis. Previously, we reported that the expression of superoxides and of the NO-generating enzyme (NO synthase, NOS) was up-regulated in the human pancreatitis, especially within the exocrine compartment indicating an exceptional susceptibility of the exocrine parenchyma to oxidative stress. The aim of the present study was to compare the regulation of NO signalling pathways in the human pancreatitis and in an animal model of an acute pancreatitis induced by pancreatic duct ligation (PDL) in rats. In the PDL-induced rat pancreatitis, we revealed a similar pattern of oxidative stress and NOS up-regulation in acinar and in ductal compartments, like in the human pancreatitis. This demonstrates that the PDL-induced rat pancreatitis is a proper model for further studies of acute pancreatitis development in humans.
Institute for Hematopathology, 22547 Hamburg, Germany. Electronic address: buchwalow@pathologie-hh.de.
Igor
I
Buchwalow
Biomedical Technology Center, Westfälische Wilhelms-Universität, Münster, 48149 Muenster, Germany.
Jürgen
J
Schnekenburger
Research Institute of Experimental Biology and Medicine, Burdenko Voronezh State Medical University, Voronezh, Russia.
Dmitri
D
Atiakshin
Institute for Hematopathology, 22547 Hamburg, Germany.
Vera
V
Samoilova
Institute for Hematopathology, 22547 Hamburg, Germany.
Eduard
E
Wolf
Institute for Hematopathology, 22547 Hamburg, Germany.
Werner
W
Boecker
Institute for Hematopathology, 22547 Hamburg, Germany.
Katharina
K
Tiemann
D000818:Animals; D006801:Humans; D007150:Immunohistochemistry; D008026:Ligation; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D018384:Oxidative Stress; D010183:Pancreatic Ducts / Q000601:surgery*; D010195:Pancreatitis / Q000503:physiopathology*; D051381:Rats
D009569:Nitric Oxide
Acute pancreatitis; Nitric oxide; Oxidative stress; · Pancreatic duct ligation ·