Sperm ultrastructure of shrimps from the family Penaeidae (Crustacea: Dendrobranchiata) in a phylogenetic context.
We describe the sperm ultrastructure of six penaeid species, including at least one member of each tribe (Penaeini, Parapenaeini and Trachypenaeini). Fragments of the vas deferens of the Penaeidae Farfantepenaeus brasiliensis, Farfantepenaeus paulensis, Litopenaeus schmitti, Parapenaeus americanus, Rimapenaeus constrictus and Xiphopenaeus kroyeri were fixed and processed according to the routine for transmission electron microscopy. The morphological results were contextualized in an evolutionary perspective using molecular markers for the phylogenetic reconstruction of this group. A phylogram was proposed by Bayesian inference based on 1007 bp of 33 sequences of the combined genes (16S rDNA and COI mtDNA) from 27 dendrobranchiate specimens. Our findings show that morphological differences in the sperm ultrastructures of members among the tribes of Penaeidae can be used as a baseline to understand their evolutionary relationships. Individuals from the Penaeini tribe show plesiomorphic characteristics in the sperm ultrastructure compared to the Trachypenaeini tribe from which they were derived, such as shrimp from family Sicyoniidae. The morphological complexity of the sperm of the different penaeid members corroborated with the genetic phylogeny, which showed different clades for each tribe and the close relationship with Sicyoniidae. The sperm features of the selected species studied here reflected their evolutionary history. These features confirm the previous phylogenetic hypothesis and question the monophyly of Penaeidae, which should be verified in the future with a more complete set of representative members of each tribe.
Laboratório de Morfologia de Invertebrados (IML), Departamento de Biologia Aplicada, CAUNESP and IEAMar, Universidade Estadual Paulista (UNESP), FCAV, Jaboticabal, SP, Brazil. Electronic address: rochatavani@gmail.com.
Tavani Rocha
TR
Camargo
Laboratório de Bioecologia e Sistemática de Crustáceos (LBSC), Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP), Universidade de São Paulo (USP), Av. Bandeirantes, 3900, 14040-901, Ribeirão Preto, SP, Brazil; Instituto de Ciências Biológicas (ICB), Universidade Federal de Goiás, Av. Esperança, s/n, Campus Samambaia (Campus II) ICB 01: sala -18, 74690-900, Goiânia, GO, Brazil.
Natalia
N
Rossi
Laboratório de Carcinologia, Departamento de Zoologia, Instituto de Biociências, Universidade Estadual Paulista (UNESP), Distrito de Rubião Júnior, S/N, 18618-970, Botucatu, SP, Brazil.
Antonio L
AL
Castilho
Laboratório de Biologia de Camarões Marinhos e de Água Doce (LABCAM), Departamento de Ciências Biológicas, Faculdade de Ciências, Universidade Estadual Paulista (UNESP), Av. Luiz Edmundo Carrijo Coube n° 14-01, Bairro Vargem Limpa, 17033-360, Bauru, SP, Brazil.
Rogério C
RC
Costa
Laboratório de Bioecologia e Sistemática de Crustáceos (LBSC), Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP), Universidade de São Paulo (USP), Av. Bandeirantes, 3900, 14040-901, Ribeirão Preto, SP, Brazil.
Fernando L
FL
Mantelatto
Laboratório de Morfologia de Invertebrados (IML), Departamento de Biologia Aplicada, CAUNESP and IEAMar, Universidade Estadual Paulista (UNESP), FCAV, Jaboticabal, SP, Brazil.
Fernando José
FJ
Zara
D000818:Animals; D001499:Bayes Theorem; D005075:Biological Evolution; D008297:Male; D033561:Penaeidae / Q000145:classification* / Q000648:ultrastructure*; D010802:Phylogeny; D013094:Spermatozoa / Q000648:ultrastructure
Bayesian inference; Decapoda; Molecular phylogeny; Sperm ultrastructure; Transmission electron microscopy