Modulatory role of Co-enzyme Q10 on methionine and choline deficient diet-induced non-alcoholic steatohepatitis (NASH) in albino rats.
The present study aimed to evaluate the hepato-protective and neuro-protective activity of Co-enzyme Q10 (CoQ10) on non-alcoholic steatohepatitis (NASH) in albino rats induced by methionine and choline-deficient (MCD) diet. Rats were fed an MCD diet for 8 weeks to induce non-alcoholic steatohepatitis. CoQ10 (10 mg/(kg·day)-1) was orally administered for 2 consecutive weeks. Twenty-four hours after the last dose of the drug, the behavioral test, namely the activity cage test, was performed and the activity counts were recorded. Serum alanine transaminase, aspartate aminotransferase, alkaline phosphatase, gamma-glutamyl transferase, total/direct bilirubin, and albumin were valued to assess liver function. Moreover, hepatic cytokines interleukin-6 as well as its modulator nuclear factor kappa-light-chain-enhancer of activated B cells were determined. In addition, brain biomarkers, viz ammonia, nitric oxide, and brain-derived neurotrophic factor (BDNF), were measured as they are reliable indices to assess brain damage. Histopathological and immunohistochemical examination of brain proliferating cell nuclear antigen in brain and liver tissues were also evaluated. Results revealed that MCD-induced NASH showed impairment in the liver functions with an increase in the liver inflammatory markers. Moreover, NASH resulted in pronounced brain dysfunction as evidenced by hyper-locomotor activity, a decrease in the BDNF level, as well as an increase in the brain nitric oxide and ammonia contents. Oral treatment of MCD-diet-fed rats with CoQ10 for 14 days showed a marked improvement in all the assigned parameters. Finally, it can be concluded that CoQ10 has a hepatoprotective and neuroprotective role in MCD-diet-induced NASH in rats.
Department of Pharmacology, Medical Division, National Research Centre, 33 EL Bohouth St., Dokki, Giza 12622, Egypt.|Department of Pharmacology, Medical Division, National Research Centre, 33 EL Bohouth St., Dokki, Giza 12622, Egypt.
Dalia O
DO
Saleh
Department of Pharmacology, Medical Division, National Research Centre, 33 EL Bohouth St., Dokki, Giza 12622, Egypt.|Department of Pharmacology, Medical Division, National Research Centre, 33 EL Bohouth St., Dokki, Giza 12622, Egypt.
Rania F
RF
Ahmed
Department of Pharmacology, Medical Division, National Research Centre, 33 EL Bohouth St., Dokki, Giza 12622, Egypt.|Department of Pharmacology, Medical Division, National Research Centre, 33 EL Bohouth St., Dokki, Giza 12622, Egypt.
Mohamed M
MM
Amin
D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000641:Ammonia / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D015415:Biomarkers / Q000097:blood; D019208:Brain-Derived Neurotrophic Factor / Q000097:blood; D002794:Choline / Q000008:administration & dosage; D002796:Choline Deficiency / Q000097:blood / Q000150:complications; D004032:Diet; D004305:Dose-Response Relationship, Drug; D015850:Interleukin-6 / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008715:Methionine / Q000008:administration & dosage / Q000097:blood / Q000172:deficiency; D018696:Neuroprotective Agents / Q000494:pharmacology; D009569:Nitric Oxide / Q000097:blood; D065626:Non-alcoholic Fatty Liver Disease / Q000097:blood / Q000188:drug therapy*; D051381:Rats; D017208:Rats, Wistar; D012709:Serum Albumin / Q000378:metabolism; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology; D005723:gamma-Glutamyltransferase / Q000097:blood
D015415:Biomarkers; D019208:Brain-Derived Neurotrophic Factor; D015850:Interleukin-6; D018696:Neuroprotective Agents; D012709:Serum Albumin; D014451:Ubiquinone; D009569:Nitric Oxide; D000641:Ammonia; D008715:Methionine; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; C024989:coenzyme Q10; D002794:Choline; D001663:Bilirubin
Co-enzyme Q10; antigène nucléaire de prolifération cellulaire; brain-derived neurotrophic factor; facteur neurotrophique dérivé du cerveau; methionine and choline deficient diet; non-alcoholic steatohepatitis; proliferating cell nuclear antigen; régime déficient en méthionine et en choline; stéatohépatite non alcoolique