Untargeted metabolomics based on ultra-high-performance liquid chromatography-high-resolution mass spectrometry merged with chemometrics: A new predictable tool for an early detection of mycotoxins.
In order to explore the early detection of mycotoxins in wheat three standardized approaches (Fusarium disease severity, PCR assays for Fusarium spp. identification and mycotoxin quantification) and a novel untargeted metabolomics strategy were jointly assessed. In the first phase of this research, standardized approaches were able to quantify mycotoxins and identify Fusarium spp. Then, an UHPLC-QTOF metabolic fingerprinting method was developed to investigate plant-pathogen cross-talk. At the same time, chemometrics analysis demonstrated to be a powerful tool in order to distinguish low and strong infection levels. Combining these results, the cross-talk plant pathogen related to the early detection of mycotoxins was discovered. As a rapid response to fungal infection an overexpression of phosphatidic acids was discovered. By contrast, when the infection became stronger an increase of oxylipins and diacylglycerols was revealed.
Department of Food Analysis and Nutrition, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, Technicka 3, 166 28 Prague 6, Czech Republic. Electronic address: rubertbj@vscht.cz.
Josep
J
Rubert
Department of Food Science, University of Parma, Parco Area delle Scienze 95/A, 43124 Parma, Italy.
Laura
L
Righetti
Department of Food Analysis and Nutrition, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, Technicka 3, 166 28 Prague 6, Czech Republic.
Milena
M
Stranska-Zachariasova
Department of Food Analysis and Nutrition, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, Technicka 3, 166 28 Prague 6, Czech Republic.
Zbynek
Z
Dzuman
Division of Crop Genetics and Breeding, Crop Research Institute, Drnovska 507, 166 28 Prague 6, Czech Republic.
Jana
J
Chrpova
Department of Food Science, University of Parma, Parco Area delle Scienze 95/A, 43124 Parma, Italy.
Chiara
C
Dall'Asta
Department of Food Analysis and Nutrition, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, Technicka 3, 166 28 Prague 6, Czech Republic. Electronic address: jana.hajslova@vscht.cz.
Jana
J
Hajslova
D002851:Chromatography, High Pressure Liquid; D004271:DNA, Fungal / Q000302:isolation & purification; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D005670:Fusarium / Q000302:isolation & purification; D013058:Mass Spectrometry; D055432:Metabolomics / Q000379:methods*; D009183:Mycotoxins / Q000032:analysis*; D025341:Principal Component Analysis; D014908:Triticum / Q000737:chemistry / Q000382:microbiology*
D004271:DNA, Fungal; D009183:Mycotoxins
Alkylresorcinols; Cereals; Lipidomics; Liquid chromatography-high resolution mass spectrometry; Metabolomics; Oxylipins; Plant-pathogen cross-talk; Wheat