Evaluation of an automated light transmission aggregometry.
Light transmission aggregometry (LTA) is the "gold standard" for platelet function assessment, but it is time-consuming and labor intensive. Recently, an automated platelet aggregation method has been developed on a routine coagulation analyzer (Sysmex CS-2100i). In this study, the performances of CS-2100i including repeatability, correlation with a reference aggregometer (Chrono-log Model 700), and the threshold limitation of platelet counts in platelet-rich plasma (PRP) were evaluated for clinical use. The agonists were adenosine diphosphate (ADP), arachidonic acid, collagen, epinephrine, and ristocetin. The platelet concentration of PRP was adjusted with platelet-poor plasma (PPP) and physiological saline (PS). The CS-2100i showed an excellent repeatability and a strong correlation with the Chrono-log 700 in performing platelet aggregation, and its threshold limitation of platelet counts in PRP was 80 × 109/L. PPP had an inhibitory impact on platelet aggregation induced by ADP, arachidonic acid, collagen or epinephrine; while PS had an inhibitory impact on ristocetin-induced aggregation. PS should be used to adjust PRP for ADP-, arachidonic acid-, collagen-, or epinephrine-induced aggregation; while PPP was recommended for ristocetin-induced aggregation. The CS-2100i showed an excellent repeatability, a strong correlation with Chrono-log 700, a lower platelet count requirement, a shorter turnaround time for samples, the advantage of being a walk-away technology, and the ability to perform a highly standardized platelet function assessment.
a West China School of Medicine/West China Hospital , Sichuan University , Chengdu , P.R. China.
Li-Qin
LQ
Ling
b Department of Laboratory Medicine , West China Hospital, Sichuan University , Chengdu , P.R. China.
Juan
J
Liao
b Department of Laboratory Medicine , West China Hospital, Sichuan University , Chengdu , P.R. China.
Qian
Q
Niu
b Department of Laboratory Medicine , West China Hospital, Sichuan University , Chengdu , P.R. China.
Xia
X
Wang
b Department of Laboratory Medicine , West China Hospital, Sichuan University , Chengdu , P.R. China.
Jin
J
Jia
b Department of Laboratory Medicine , West China Hospital, Sichuan University , Chengdu , P.R. China.
Cheng-Hua
CH
Zuo
b Department of Laboratory Medicine , West China Hospital, Sichuan University , Chengdu , P.R. China.
Hong
H
Jiang
b Department of Laboratory Medicine , West China Hospital, Sichuan University , Chengdu , P.R. China.
Jing
J
Zhou
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000293:Adolescent; D000328:Adult; D016718:Arachidonic Acid / Q000494:pharmacology; D057205:Automation, Laboratory / Q000295:instrumentation / Q000379:methods / Q000592:standards*; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects*; D003094:Collagen / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D008297:Male; D008875:Middle Aged; D010974:Platelet Aggregation / Q000187:drug effects*; D010979:Platelet Function Tests / Q000592:standards*; D053657:Platelet-Rich Plasma / Q000166:cytology; D015203:Reproducibility of Results; D012310:Ristocetin / Q000494:pharmacology
D012310:Ristocetin; D016718:Arachidonic Acid; D000244:Adenosine Diphosphate; D003094:Collagen; D004837:Epinephrine
Automation; platelet aggregation; platelet count; platelet function tests; platelet-rich plasma