Cytogenetic Biomonitoring in Buccal Mucosal Cells from Municipal Solid Waste Collectors.
Waste collectors collect, transport, and process the garbage produced by people living in the city. Nowadays, this activity requires special attention due to the environmental impact of garbage and its potential consequences on human health. The aim of this study was to evaluate potential cytotoxic and mutagenic effects of garbage collection on waste collectors. For this purpose, a total of 47 male waste collectors aged from 24 to 53 years were included in the experimental group. A total of 30 men matched by age were used as the control group. Cytotoxicity and mutagenicity were analyzed by micronucleus test in buccal mucosaI cells. No statistically significant difference (p>0.05) in the frequency of micronuclei was detected in the waste collectors when compared to controls. Nevertheless, higher frequencies of karyolysis and pyknosis (p<0.05) were detected in buccal mucosaI cells from waste collectors when compared to matched controls. Taken together, our results indicate that waste collectors comprise an at-risk group as a result of increased cytotoxicity apparent from buccal mucosa cells.
Post-graduate Program in Dentistry and Health, Federal University of Bahia, UFBA, Salvador, Brazil.
Mariana Carvalho
MC
Andrade
Post-graduate Program in Dentistry and Health, Federal University of Bahia, UFBA, Salvador, Brazil.
Jean Nunes
JN
Dos Santos
Post-graduate Program in Dentistry and Health, Federal University of Bahia, UFBA, Salvador, Brazil.
Patricia Ramos
PR
Cury
Department of Biosciences, Federal University of Sao Paulo, UNIFESP, Santos, Brazil.
Ana Carolina Correa
AC
Flygare
Department of Biosciences, Federal University of Sao Paulo, UNIFESP, Santos, Brazil.
Samuel Rangel
SR
Claudio
Department of Pathology, Paulista Medical School, UNIFESP, São Paulo, Brazil.
Celina Tizuko Fujiyama
CT
Oshima
Department of Biosciences, Federal University of Sao Paulo, UNIFESP, Santos, Brazil daribeiro@unifesp.br daribeiro@pesquisador.cnpq.br.
Daniel Araki
DA
Ribeiro
D000328:Adult; D002467:Cell Nucleus / Q000187:drug effects / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects; D020732:Cytogenetic Analysis / Q000379:methods*; D004784:Environmental Monitoring / Q000379:methods*; D015386:Hazardous Substances / Q000032:analysis / Q000494:pharmacology; D006801:Humans; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects; D015162:Micronucleus Tests / Q000379:methods; D008875:Middle Aged; D009061:Mouth Mucosa / Q000166:cytology / Q000378:metabolism*; D016273:Occupational Exposure / Q000032:analysis; D016272:Occupational Health / Q000592:standards / Q000706:statistics & numerical data*; D062611:Solid Waste / Q000032:analysis*; D055815:Young Adult
D015386:Hazardous Substances; D062611:Solid Waste
Oral mucosa cells; biomontoring; micronucleus test; waste collectors