Assessment of Natural Variability of Maize Lipid Transfer Protein Using a Validated Sandwich ELISA.
Lipid transfer protein (LTP) is the main causative agent for rare food allergic reactions to maize. This paper describes a new, validated ELISA that accurately measures maize LTP concentrations from 0.2 to 6.4 ng/mL. The levels of LTP ranged from 171 to 865 μg/g of grain, a 5.1-fold difference, across a set of 49 samples of maize B73 hybrids derived from the Nested Association Mapping (NAM) founder lines and a diverse collection of landrace accessions from North and South America. A second set of 107 unique samples from 18 commercial hybrids grown over two years across 10 U.S. states showed a comparable range of LTP level (212-751 μg/g of grain). Statistical analysis showed that genetic and environmental factors contributed 63 and 6%, respectively, to the variance in LTP levels. Therefore, the natural variation of maize LTP is up to 5-fold different across a diverse collection of varieties that have a history of safe cultivation and consumption.
Monsanto Company , 800 North Lindbergh Boulevard, St. Louis, Missouri 63167, United States.
Xin
http://orcid.org/0000-0001-8194-0309
X
Gu
Monsanto Company , 800 North Lindbergh Boulevard, St. Louis, Missouri 63167, United States.
Thomas
T
Lee
Monsanto Company , 800 North Lindbergh Boulevard, St. Louis, Missouri 63167, United States.
Tao
http://orcid.org/0000-0002-7367-4693
T
Geng
Monsanto Company , 800 North Lindbergh Boulevard, St. Louis, Missouri 63167, United States.
Kang
K
Liu
Monsanto Company , 800 North Lindbergh Boulevard, St. Louis, Missouri 63167, United States.
Richard
R
Thoma
Vasculox , 4320 Forest Park Avenue, Suite 304, St. Louis, Missouri 63108, United States.
Kathleen
K
Crowley
Monsanto Company , 800 North Lindbergh Boulevard, St. Louis, Missouri 63167, United States.
Thomas
T
Edrington
Royal Canin USA , 500 Fountain Lakes Boulevard, Suite 100, St. Charles, Missouri 63301, United States.
Jason M
JM
Ward
Monsanto Company , 800 North Lindbergh Boulevard, St. Louis, Missouri 63167, United States.
Yongcheng
Y
Wang
Agricultural Research Service, U.S. Department of Agriculture , Columbia, Missouri 65211, United States.|Division of Plant Sciences, University of Missouri , Columbia, Missouri 65211, United States.
Sherry
S
Flint-Garcia
Monsanto Company , 800 North Lindbergh Boulevard, St. Louis, Missouri 63167, United States.
Erin
E
Bell
Monsanto Company , 800 North Lindbergh Boulevard, St. Louis, Missouri 63167, United States.
Kevin C
http://orcid.org/0000-0002-7600-2550
KC
Glenn
D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D010940:Plant Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D003313:Zea mays / Q000737:chemistry* / Q000235:genetics / Q000276:immunology
D002352:Carrier Proteins; D010940:Plant Proteins; C030363:lipid transfer protein
ELISA; LTP; lipid transfer protein; maize; natural variation