Clinical outcomes after IVF or ICSI using human blastocysts derived from oocytes containing aggregates of smooth endoplasmic reticulum.
In this study the clinical and neo-natal outcomes after transfer of blastocysts derived from oocytes containing aggregates of smooth endoplasmic reticulum (SER) were compared between IVF and intracytoplasmic sperm injection (ICSI) cycles. Clinical and neo-natal outcomes of blastocysts in cycles with at least one SER metaphase II oocyte (SER + MII; SER + cycles) did not significantly differ between the two insemination methods. When SER + MII were cultured to day 5/6, fertilization, embryo cleavage and blastocyst rates were not significantly different between IVF and ICSI cycles. In vitrified-warmed blastocyst transfer cycles, the clinical pregnancy rates from SER + MII in IVF and ICSI did not significantly differ. In this study, 52 blastocysts (27 IVF and 25 ICSI) derived from SER + MII were transferred, yielding 15 newborns (5 IVF and 10 ICSI) and no malformations. Moreover, 300 blastocysts (175 IVF and 125 ICSI) derived from SER-MII were transferred, yielding 55 newborns (24 IVF and 31 ICSI cycles). Thus, blastocysts derived from SER + cycles exhibited an acceptable ongoing pregnancy rate after IVF (n = 125) or ICSI (n = 117) cycles. In conclusion, blastocysts from SER + MII in both IVF and ICSI cycles yield adequate ongoing pregnancy rates with neo-natal outcomes that do not differ from SER-MII.
Department of Infertility, Green Bell Clinic, Kitamachi 2-160, Toyota, Aichi 471-0027, Japan; Faculty of Life and Environmental Sciences, University of Yamanashi, Takeda 4-4-37, Kofu, Yamanashi 400-8510, Japan. Electronic address: fumiaki-itoi@kishokai.or.jp.
Fumiaki
F
Itoi
Department of Infertility, Royal Bell Clinic, Mizuhiroge 93-195, Narumi-cho, Midori-ku, Nagoya, Aichi 458-0801, Japan.
Yukiko
Y
Asano
Department of Infertility, Angel Bell Hospital, Nishiki-machi 5-1, Okazaki, Aichi 444-0067, Japan.
Masashi
M
Shimizu
Department of Infertility, Royal Bell Clinic, Mizuhiroge 93-195, Narumi-cho, Midori-ku, Nagoya, Aichi 458-0801, Japan.
Rika
R
Nagai
Department of Infertility, Angel Bell Hospital, Nishiki-machi 5-1, Okazaki, Aichi 444-0067, Japan.
Kanako
K
Saitou
Sapporo ART Clinic, Kita7jonishi 4-1-2, Kita-ku, Sapporo, Hokkaido 060-0807, Japan.
Hiroyuki
H
Honnma
Department of Infertility, Angel Bell Hospital, Nishiki-machi 5-1, Okazaki, Aichi 444-0067, Japan.
Yasutaka
Y
Murata
D000328:Adult; D001755:Blastocyst / Q000166:cytology / Q000648:ultrastructure; D004624:Embryo Transfer; D047108:Embryonic Development; D018871:Endoplasmic Reticulum, Smooth / Q000648:ultrastructure*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D009865:Oocytes / Q000648:ultrastructure*; D011247:Pregnancy; D018873:Pregnancy Rate; D020554:Sperm Injections, Intracytoplasmic
Aggregates of smooth endoplasmic reticulum; Clinical outcome; ICSI; IVF; Neo-natal outcome; Vitrified–warmed blastocyst transfer