Direct conversion of root primordium into shoot meristem relies on timing of stem cell niche development.
To understand how the identity of an organ can be switched, we studied the transformation of lateral root primordia (LRP) into shoot meristems inArabidopsisroot segments. In this system, the cytokinin-induced conversion does not involve the formation of callus-like structures. Detailed analysis showed that the conversion sequence starts with a mitotic pause and is concomitant with the differential expression of regulators of root and shoot development. The conversion requires the presence of apical stem cells, and only LRP at stages VI or VII can be switched. It is engaged as soon as cell divisions resume because their position and orientation differ in the converting organ compared with the undisturbed emerging LRP. By alternating auxin and cytokinin treatments, we showed that the root and shoot organogenetic programs are remarkably plastic, as the status of the same plant stem cell niche can be reversed repeatedly within a set developmental window. Thus, the networks at play in the meristem of a root can morph in the span of a couple of cell division cycles into those of a shoot, and back, through transdifferentiation.
Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, Versailles 78000, France.|Sorbonne Universités, UPCM Université Paris 06, UFR 927, Paris F-75005, France.
Olga
O
Rosspopoff
Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, Versailles 78000, France.
Liudmila
L
Chelysheva
Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, Versailles 78000, France.|Université Paris-Diderot, Sorbonne Paris Cité, Paris F-75205, France.
Julie
J
Saffar
Sorbonne Universités, UPCM Université Paris 06, UFR 927, Paris F-75005, France.
Lena
L
Lecorgne
Muséum d'Histoire Naturelle, UMS 2700, OMSI, Paris F-75231, France.
Delphine
D
Gey
Institut de Biologie de l'Ecole Normale Supérieure, CNRS UMR8197, INSERM U1024, Paris F-75005, France.
Erwann
E
Caillieux
Institut de Biologie de l'Ecole Normale Supérieure, CNRS UMR8197, INSERM U1024, Paris F-75005, France.
Vincent
V
Colot
Institut de Biologie de l'Ecole Normale Supérieure, CNRS UMR8197, INSERM U1024, Paris F-75005, France.
François
F
Roudier
Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, Versailles 78000, France pierre.hilson@inra.fr marco.da-costa@inra.fr philippe.rech@inra.fr.
Pierre
P
Hilson
LIPM, Université de Toulouse, INRA, CNRS, INPT, Castanet-Tolosan F-31126, France.|CNRS, Laboratoire des Interactions Plantes-Microorganismes, UMR2594, Castanet-Tolosan 31326, France.|Plant Genomics Research Unit, UMR INRA 1165 - CNRS 8114 - UEVE, 2, CP5708, Evry Cedex 91057, France.
Richard
R
Berthomé
Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, Versailles 78000, France pierre.hilson@inra.fr marco.da-costa@inra.fr philippe.rech@inra.fr.|Sorbonne Universités, UPCM Université Paris 06, UFR 927, Paris F-75005, France.
Marco
M
Da Costa
Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, Versailles 78000, France pierre.hilson@inra.fr marco.da-costa@inra.fr philippe.rech@inra.fr.|Sorbonne Universités, UPCM Université Paris 06, UFR 927, Paris F-75005, France.
Philippe
0000-0002-6631-9888
P
Rech
D017360:Arabidopsis / Q000166:cytology* / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D002455:Cell Division / Q000187:drug effects; D054338:Cell Transdifferentiation / Q000187:drug effects; D003583:Cytokinins / Q000494:pharmacology; D019175:DNA Methylation / Q000187:drug effects / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018519:Meristem / Q000166:cytology* / Q000187:drug effects; D063245:Plant Development / Q000187:drug effects; D010937:Plant Growth Regulators / Q000378:metabolism; D055153:Stem Cell Niche / Q000187:drug effects; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects
D003583:Cytokinins; D010937:Plant Growth Regulators
Arabidopsis; Regeneration; Root-to-shoot conversion; Stem cell niche; Totipotency; Transdifferentiation