Prevalence ofStaphylococcus aureusNasal Carriage in Human Immunodeficiency Virus-Infected and Uninfected Children in Botswana: Prevalence and Risk Factors.
AbstractStaphylococcus aureusis an important cause of morbidity and mortality in children in sub-Saharan Africa (SSA). A major risk factor for staphylococcal infection isS. aureuscolonization of the anterior nares. We sought to define risk factors forS. aureuscarriage and characterize antimicrobial resistance patterns in children in Botswana. A cross-sectional study was conducted at two clinical sites in southern Botswana. Patients under 18 years of age underwent two nasal swabs and brief interviews, 4 weeks apart. Standard microbiological techniques were used. For persistent carriers,S. aureuswas isolated from swabs at both time points, and for intermittent carriers,S. aureuswas isolated from only one swab. Poisson regression with robust variance estimator was used to compare prevalence of carriage and the resistance phenotypes. Among 56 enrollees, prevalence ofS. aureuscolonization was 55% (N= 31), of whom 42% (N= 13) were persistent carriers. Of human immunodeficiency virus-infected children, 64% (N= 9) were carriers. Risk factors for nasal carriage included a history of tuberculosis (prevalence ratio [PR] = 1.60; 95% confidence interval [CI] = 1.02, 2.51;P= 0.040) and closer proximity to health care (PR = 0.89; 95% CI = 0.80, 0.99;P= 0.048). Prior pneumonia was more common among persistent rather than intermittent carriers (PR = 2.64; 95% CI = 1.64, 4.23;P< 0.001). Methicillin-resistantS. aureus(MRSA) prevalence was 13%. Of isolates tested, 16% were resistant to three or more drugs (N= 7/44). In summary, children in southern Botswana are frequently colonized withS. aureus. Antibiotic resistance, especially MRSA, is also widespread. Antibiotic recommendations for treatment of staphylococcal infections in SSA should take cognizance of these resistance patterns.
University of California San Francisco, San Francisco, California.
Michael J A
MJA
Reid
The University of Texas Health Science Center School of Public Health, Houston, Texas.|National School of Tropical Medicine, Baylor College of Medicine, Houston, Texas.
Rebecca S B
RSB
Fischer
University of Botswana, Gaborone, Botswana.
Naledi
N
Mannathoko
Botswana-UPenn Partnership, Gaborone, Botswana.
Charles
C
Muthoga
The University of Texas Health Science Center School of Public Health, Houston, Texas.
Erin
E
McHugh
The University of Texas Health Science Center School of Public Health, Houston, Texas.
Heather
H
Essigmann
The University of Texas Health Science Center School of Public Health, Houston, Texas.
Eric L
EL
Brown
Children's Hospital of Philadelphia, University of Pennsylvania, Philadelphia, Pennsylvania.|Botswana-UPenn Partnership, Gaborone, Botswana.|University of Botswana, Gaborone, Botswana.
Andrew P
AP
Steenhoff
D000293:Adolescent; D001902:Botswana / Q000453:epidemiology; D002353:Carrier State; D002648:Child; D002675:Child, Preschool; D005260:Female; D015658:HIV Infections / Q000150:complications* / Q000453:epidemiology; D006801:Humans; D007223:Infant; D008297:Male; D009666:Nose / Q000382:microbiology*; D015995:Prevalence; D012307:Risk Factors; D013211:Staphylococcus aureus / Q000302:isolation & purification*