Sporicidal efficacy of thermal-sprayed copper alloy coating.
Approximately 200 000 Canadians acquire healthcare-associated bacterial infections each year and several-fold more acquire food-borne bacterial illnesses. Bacterial spores are particularly problematic because they can survive on surfaces for several months. Owing to its sporicidal activity, copper alloy sheet metal is sometimes used in hospital settings, but its widespread use is limited by cost and incompatibility with complex furniture and instrument designs and topographies. A potential alternative is the use of thermal spray technology to coat surfaces with copper alloys. We compared the sporicidal activity of thermally sprayed copper alloy on stainless steel with that of copper alloy sheet metal against Bacillus subtilis spores. Spores remained intact for at least 1 week on uncoated stainless steel, whereas spore fragmentation was initiated within 2 h of exposure to either copper surface. Less than 15% of spores were viable 2 h after exposure to either copper surface, as compared with stainless steel. By day 7, only degraded spores and petal-like nanoflowers were present on the copper surfaces. Nanoflowers, which are laminar arrangements of thin crystal sheets composed of carbon - copper phosphate, appeared to be derived from the degraded spores. Altogether, these results indicate that a thermal-sprayed copper alloy coating on stainless steel provides sporicidal activity similar to that afforded by copper alloy sheet metal.
a Centre for Advanced Coating Technologies, Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, ON M5S 3G8, Canada.
Romina
R
Shafaghi
a Centre for Advanced Coating Technologies, Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, ON M5S 3G8, Canada.
Javad
J
Mostaghimi
a Centre for Advanced Coating Technologies, Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, ON M5S 3G8, Canada.
Valerian
V
Pershin
b Department of Cell and Systems Biology, University of Toronto, Toronto, ON M5S 3G5, Canada.
Maurice
M
Ringuette
D000497:Alloys; D001412:Bacillus subtilis / Q000187:drug effects*; D002170:Canada; D003300:Copper / Q000494:pharmacology*; D004202:Disinfectants / Q000494:pharmacology*; D013171:Spores, Bacterial / Q000187:drug effects*; D013193:Stainless Steel
D000497:Alloys; D004202:Disinfectants; D013193:Stainless Steel; D003300:Copper
Bacillus subtilis spores; focused ion beam sectioning; nanofleurs; nanoflowers; revêtement d’alliage de cuivre pulvérisé thermiquement; sectionnement par faisceaux d’ions focalisés; spores de Bacillus subtilis; sporicidal; sporicide; thermal-sprayed copper alloy coating