Resonance light scattering method for detecting kanamycin in milk with enhanced sensitivity.
In this paper, a resonance light scattering (RLS) method was developed for detecting kanamycin with high sensitivity and selectivity. Here, aptamer specific to kanamycin was utilized for recognizing and competitively binding with kanamycin, and gold nanoparticles (GNPs) were used as probes. After adding kanamycin into solutions containing aptamer and GNPs, the GNPs would aggregate in the presence of NaCl, which resulted in the RLS signal intensities enhanced dramatically. Based on this phenomenon, kanamycin can be detected in the range of 10 to 600 nM with a limit of detection as 1 nM, which is more sensitive than many other instrumental methods, especially the commonly used UV-visible spectroscopic method. Furthermore, we demonstrated that this method can be used for detecting kanamycin in milk samples with satisfactory results, which is meaningful for solving food safety problems.
College of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu, 212013, China. wangck@ujs.edu.cn.
Chengke
C
Wang
College of Life Sciences, Sichuan University, No.29 Jiuyanqiao Wangjiang Road, Chengdu, Sichuan, 610064, China.
Chengen
C
Wang
College of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu, 212013, China.
Qingqing
Q
Wang
College of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu, 212013, China.
Dan
D
Chen
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis; D000067493:Dynamic Light Scattering / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D007118:Immunoassay / Q000379:methods*; D007612:Kanamycin / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D015203:Reproducibility of Results; D052156:SELEX Aptamer Technique / Q000379:methods*; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D007612:Kanamycin
Kanamycin; Milk, aptamer, gold nanoparticle; Resonance light scattering