Expanding Proteome Coverage with CHarge Ordered Parallel Ion aNalysis (CHOPIN) Combined with Broad Specificity Proteolysis.
The "deep" proteome has been accessible by mass spectrometry for some time. However, the number of proteins identified in cells of the same type has plateaued at ∼8000-10 000 without ID transfer from reference proteomes/data. Moreover, limited sequence coverage hampers the discrimination of protein isoforms when using trypsin as standard protease. Multienzyme approaches appear to improve sequence coverage and subsequent isoform discrimination. Here we expanded proteome and protein sequence coverage in MCF-7 breast cancer cells to an as yet unmatched depth by employing a workflow that addresses current limitations in deep proteome analysis in multiple stages: We used (i) gel-aided sample preparation (GASP) and combined trypsin/elastase digests to increase peptide orthogonality, (ii) concatenated high-pH prefractionation, and (iii) CHarge Ordered Parallel Ion aNalysis (CHOPIN), available on an Orbitrap Fusion (Lumos) mass spectrometer, to achieve 57% median protein sequence coverage in 13 728 protein groups (8949 Unigene IDs) in a single cell line. CHOPIN allows the use of both detectors in the Orbitrap on predefined precursor types that optimizes parallel ion processing, leading to the identification of a total of 179 549 unique peptides covering the deep proteome in unprecedented detail.
Target Discovery Institute, Nuffield Department of Medicine, University of Oxford , Roosevelt Drive, Oxford OX3 7FZ, United Kingdom.
Simon
0000-0001-7840-2411
S
Davis
Target Discovery Institute, Nuffield Department of Medicine, University of Oxford , Roosevelt Drive, Oxford OX3 7FZ, United Kingdom.
Philip D
PD
Charles
Bioinformatics Solutions, Inc. , 470 Weber Street North Suite 204, Waterloo, Ontario N2L 6J2, Canada.
Lin
L
He
Thermo Fisher, Inc. , Stafford House, 1 Boundary Park, Hemel Hampstead HP2 7GE, United Kingdom.
Peter
P
Mowlds
Target Discovery Institute, Nuffield Department of Medicine, University of Oxford , Roosevelt Drive, Oxford OX3 7FZ, United Kingdom.
Benedikt M
BM
Kessler
Target Discovery Institute, Nuffield Department of Medicine, University of Oxford , Roosevelt Drive, Oxford OX3 7FZ, United Kingdom.
Roman
0000-0002-9715-5951
R
Fischer
D000595:Amino Acid Sequence; D006801:Humans; D061986:MCF-7 Cells; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000032:analysis; D059748:Proteolysis; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods* / Q000592:standards
D010455:Peptides; D020543:Proteome
LC−MS/MS; deep proteome; isoform profiling; protein sequence coverage; sequence coverage