A facile carbon dots based fluorescent probe for ultrasensitive detection of ascorbic acid in biological fluids via non-oxidation reduction strategy.
A rapid, facile and ultrasensitive fluorescence sensing system based on nitrogen-doped carbon dots (N-doped CDs) for the detection of ascorbic acid (AA) was developed. The highly photoluminescent N-doped CDs with excellent solubility in water and good biocompatibility were prepared by a one-step hydrothermal synthesis and gave the fluorescence quantum yield of 47%. The addition of AA can intensively suppress the fluorescence of the N-doped CDs through the synergistic effect of the inner filter effect (IFE) and the static quenching effect (SQE). Benefited from the remarkable synergistic effect of IFE and SQE, a facile and ultrasensitive sensor was constructed successfully for AA sensing. The detection procedure was achieved within 2min. The linear response range of AA was obtained from 10-3to 10-8M with a detection limit of 5nM. This developed method enjoyed many merits including more simplicity, lost cost, high sensitivity, good selectivity, rapid response and excellent biocompatibility. Notably, the proposed fluorescent sensor exhibits excellent performance and applicability for AA determination in human serum and rat brain microdialysate, and may provide a fast yet facile route for AA detection in physiological and pathological fields.
Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu 273165, China.
Weiheng
W
Kong
School of Life Sciences, Xiamen University, Xiamen 361005, China.
Di
D
Wu
Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu 273165, China; Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Centre for Food Safety Risk Assessment, Beijing 100021, China. Electronic address: 61254368@163.com.
Guoliang
G
Li
School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xian 710021, China.
Xuefeng
X
Chen
Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu 273165, China.
Peiwei
P
Gong
Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu 273165, China.
Zhiwei
Z
Sun
Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu 273165, China.
Guang
G
Chen
Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu 273165, China.
Lian
L
Xia
Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu 273165, China.
Jinmao
J
You
Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Centre for Food Safety Risk Assessment, Beijing 100021, China. Electronic address: wuyongning@cfsa.net.cn.
Yongning
Y
Wu
D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D001921:Brain / Q000378:metabolism*; D002244:Carbon / Q000737:chemistry*; D016022:Case-Control Studies; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D064368:Healthy Volunteers; D006801:Humans; D057230:Limit of Detection; D008297:Male; D017551:Microdialysis; D009369:Neoplasms / Q000097:blood*; D045663:Quantum Dots; D051381:Rats; D017207:Rats, Sprague-Dawley; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D002244:Carbon; D001205:Ascorbic Acid
Ascorbic acid; Carbon dots; Fluorimetric detection; Inner filter effect