Genomic Characterization of Two KPC-Producing Klebsiella Isolates Collected in 1997 in New York City.
Klebsiella pneumoniaecarbapenemase (KPC)-producingEnterobacteriaceaehave now become a global public health threat. However, the origin of this pandemic and the characterization of pre-2003blaKPC-harboring plasmids remain unknown. Here we used next-generation sequencing to characterize two KPC-2-producingK. pneumoniaeandKmichiganensisisolates collected from a New York City hospital in 1997. Although identified in two differentKlebsiellaspecies, theblaKPC-2gene was harbored by Tn4401btransposons on two highly similar IncN plasmids.
Division of Infectious Diseases, Department of Medicine, State University of New York Downstate, New York, New York, USA Brandon.Eilertson@Downstate.edu.
Brandon
B
Eilertson
Public Health Research Institute Tuberculosis Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA.
Liang
L
Chen
Public Health Research Institute Tuberculosis Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA.
Kalyan D
KD
Chavda
Public Health Research Institute Tuberculosis Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA.
Barry N
BN
Kreiswirth
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015780:Carbapenems / Q000494:pharmacology; D004251:DNA Transposable Elements / Q000235:genetics; D004196:Disease Outbreaks; D006761:Hospitals; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D008826:Microbial Sensitivity Tests; D009519:New York City; D010957:Plasmids / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D004251:DNA Transposable Elements; D001618:beta-Lactamases; C063912:carbapenemase
CRE; KPC; Tn4401b; carbapenems