Structural characterization of an all-aminosugar-containing capsular polysaccharide from Colwellia psychrerythraea 34H.
Colwellia psychrerythraea strain 34H, a Gram-negative bacterium isolated from Arctic marine sediments, is considered a model to study the adaptation to cold environments. Recently, we demonstrated that C. psychrerythraea 34H produces two different extracellular polysaccharides, a capsular polysaccharide and a medium released polysaccharide, which confer cryoprotection to the bacterium. In this study, we report the structure of an additional capsular polysaccharide produced by Colwellia grown at a different temperature. The structure was determined using chemical methods, and one- and two-dimensional NMR spectroscopy. The results showed a trisaccharide repeating unit made up of only amino-sugar residues: N-acetyl-galactosamine, 2,4-diacetamido-2,4,6-trideoxy-glucose (bacillosamine), and 2-acetamido-2-deoxyglucuronic acid with the following structure: →4)-β-D-GlcpNAcA-(1 →3)-β-D-QuipNAc4NAc-(1 →3)-β-D-GalpNAc-(1 →. The 3D model, generated in accordance with1H,1H-NOE NMR correlations and consisting of ten repeating units, shows a helical structure. In contrast with the other extracellular polysaccharides produced from Colwellia at 4 °C, this molecule displays only a low ice recrystallization inhibition activity.
Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126, Naples, Italy.
Angela
A
Casillo
Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, S-106 91, Stockholm, Sweden.
Jonas
J
Ståhle
Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126, Naples, Italy.
Ermenegilda
E
Parrilli
Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126, Naples, Italy.
Filomena
F
Sannino
Department of Chemistry and Warwick Medical School, University of Warwick, Coventry, CV4 7AL, UK.
Daniel E
DE
Mitchell
Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126, Naples, Italy.
Giuseppina
G
Pieretti
Department of Chemistry and Warwick Medical School, University of Warwick, Coventry, CV4 7AL, UK.
Matthew I
MI
Gibson
Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126, Naples, Italy.
Gennaro
G
Marino
Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126, Naples, Italy.
Rosa
R
Lanzetta
Department of Biology, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126, Naples, Italy.
Michelangelo
M
Parrilli
Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, S-106 91, Stockholm, Sweden.
Göran
G
Widmalm
Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126, Naples, Italy.
Maria L
ML
Tutino
Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126, Naples, Italy. corsaro@unina.it.
Maria M
http://orcid.org/0000-0002-6834-8682
MM
Corsaro
D000222:Adaptation, Physiological; D034241:Alteromonadaceae / Q000737:chemistry* / Q000166:cytology; D000606:Amino Sugars / Q000737:chemistry*; D021301:Antifreeze Proteins / Q000737:chemistry* / Q000302:isolation & purification; D002240:Carbohydrate Sequence; D003080:Cold Temperature; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008968:Molecular Conformation; D056004:Molecular Dynamics Simulation; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification
D000606:Amino Sugars; D021301:Antifreeze Proteins; D011135:Polysaccharides, Bacterial
Anti-freeze activity; Cold adaptation; Extracellular polysaccharides; NMR; Psychrophile