Application of bacteria from non-cultivated plants to promote growth, alter root architecture and alleviate salt stress of cotton.
OBJECTIVE
Cotton seeds are frequently treated with acid to remove fibres and reduce seed-transmitted diseases. This process also eliminates beneficial bacteria on the seed surface. The goal of this research was to seek and apply beneficial bacteria to acid delinted cotton seeds to evaluate their growth-promoting and salt stress alleviating effects in seedlings.


RESULTS
Bacteria were isolated from non-cultivated plants in the Malvaceae. Seeds were collected from Portia tree (Thespesia populnea) and wild cotton (Gossypium hirsutum) from coastal and arid areas of Puerto Rico. Bacillus amyloliquefaciens, Curtobacterium oceanosedimentum and Pseudomonas oryzihabitans were inoculated onto acid delinted cotton seeds. Bacteria increased cotton seed germination and length of emerging seedling radicles. Cotton seeds were inoculated with B. amyloliquefaciens to evaluate growth and root architecture of non-stressed and salt stressed seedlings. Inoculating cotton seeds with B. amyloliquefaciens led to a greater percentage of seedlings with expanded cotyledons after 8 days, enhanced primary and lateral root growth, and altered root architecture. Similar results were obtained when okra seeds were inoculated with B. amyloliquefaciens.


CONCLUSIONS
The data supported the hypothesis that non-cultivated plants in the Malvaceae growing in stressful environments possess bacteria that promote growth, alter root architecture and alleviate salt stress of cotton and okra seedlings.


CONCLUSIONS
This study demonstrated the effects of applying beneficial bacteria on acid delinted cotton seeds. Inoculating seeds with salt stress alleviating bacteria could improve the growth of crop seedlings that are vulnerable to soil salinization.
Department of Plant Biology, Rutgers University, New Brunswick, NJ, USA.
I
I
Irizarry
Department of Plant Biology, Rutgers University, New Brunswick, NJ, USA.
J F
JF
White
D001419:Bacteria / Q000302:isolation & purification; D018407:Bacterial Physiological Phenomena; D018525:Germination; D003368:Gossypium / Q000033:anatomy & histology / Q000254:growth & development* / Q000382:microbiology*; D018517:Plant Roots / Q000033:anatomy & histology / Q000254:growth & development; D054712:Salinity; D036226:Seedlings / Q000254:growth & development; D012639:Seeds / Q000382:microbiology; D013312:Stress, Physiological

Bacillus
; cotton; growth promotion; root architecture; salt stress