New design of experiment combined with UV-Vis spectroscopy for extraction and estimation of polyphenols from Basil seeds, Red seeds, Sesame seeds and Ajwan seeds.
New experimental designs for the extraction of polyphenols from different seeds including Basil seed, Red seed, Sesame seeds and Ajwan seeds were investigated. Four variables the concentration and volume of methanol and NaOH solutions as well as the temperature and time of extraction were varied to see their effect on total phenol extraction. The temperature was varied in the range from 25°C to 200°C while the time in the range from 30 to 200minutes. Response surface methodology was used to optimize the extraction parameters. The estimation of polyphenols was measured through phenols reduction UV-Vis spectroscopic method of phosphotungstic-phosphomolybdic acids (Folin-Ciocalteu's reagent). Calibration curve was made by using tannic acid as a polyphenols standard in the concentration range from 0.1 to 10ppm. The regression line obtained shows the value of correlation coefficient i.e. R=0.930 and Root mean square error of cross validation (RMSEC) value of 0.0654. The Basil seeds were found containing the highest amount of total phenols i.e. 785.76mg/100g. While the Sesame seeds having the least amount i.e. 33.08mg/100g. The Ajwan seeds and the Red seeds are containing the medium amounts i.e. 379mg/100g and 220.54mg/100g respectively.
Department of Biological Sciences & Chemistry, College of Arts and Sciences, University of Nizwa, Oman. Electronic address: mehboob@unizwa.edu.om.
Fazal
F
Mabood
Department of Biological Sciences & Chemistry, College of Arts and Sciences, University of Nizwa, Oman. Electronic address: gilani@unizwa.edu.om.
Syed Abdullah
SA
Gilani
Department of Biological Sciences & Chemistry, College of Arts and Sciences, University of Nizwa, Oman.
Javid
J
Hussain
Department of Biological Sciences & Chemistry, College of Arts and Sciences, University of Nizwa, Oman.
Sulaiman
S
Alshidani
Department of Biological Sciences & Chemistry, College of Arts and Sciences, University of Nizwa, Oman.
Said
S
Alghawi
Department of Biological Sciences & Chemistry, College of Arts and Sciences, University of Nizwa, Oman.
Mohammed
M
Albroumi
Department of Biological Sciences & Chemistry, College of Arts and Sciences, University of Nizwa, Oman.
Saif
S
Alameri
Department of Chemistry, University of Malakand, KPK, Pakistan.
Farah
F
Jabeen
Department of Chemistry, Abdul Wali Khan University, KPK, Pakistan.
Zahid
Z
Hussain
UoN Chair of Oman Medicinal Plants and Marine Products, University of Nizwa, Oman.
Ahmed
A
Al-Harrasi
Department of Biological Sciences & Chemistry, College of Arts and Sciences, University of Nizwa, Oman.
Zahra K M
ZKM
Al Abri
Department of Biological Sciences & Chemistry, College of Arts and Sciences, University of Nizwa, Oman.
Saima
S
Farooq
Department of Biological Sciences & Chemistry, College of Arts and Sciences, University of Nizwa, Oman.
Zakira
Z
Naureen
Department of Biological Sciences & Chemistry, College of Arts and Sciences, University of Nizwa, Oman.
Ahmad
A
Hamaed
Institute of Chemical Sciences, University of Peshawar, KPK, Pakistan.
M
M
Rasul Jan
Institute of Chemical Sciences, University of Peshawar, KPK, Pakistan.
Jasmin
J
Shah
D000704:Analysis of Variance; D019661:Apiaceae / Q000737:chemistry*; D002138:Calibration; D029686:Lepidium / Q000737:chemistry*; D018649:Ocimum basilicum / Q000737:chemistry*; D059808:Polyphenols / Q000032:analysis* / Q000302:isolation & purification*; D012015:Reference Standards; D012639:Seeds / Q000737:chemistry*; D031685:Sesamum / Q000737:chemistry*; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D059808:Polyphenols; D012996:Solutions
Ajwan seeds; Basil seed; Design of Experiment (DoE); Extraction of phenols; Red seed; Sesame seeds; UV-VIS spectroscopy