Hypoglycemic effects of Prunus cerasus L. pulp and seed extracts on Alloxan-Induced Diabetic Mice with histopathological evaluation.
OBJECTIVE
The study was undertaken to investigate the acute and subchronic hypoglycemic effects of an ethyl acetate (EtAc) extract of red sour cherry (Prunus cerasus) pulps and seeds in alloxan-induced diabetic mice.


METHODS
The Prunus cerasus pulp (PPc) and seed (SPc) EtAc extract s were IP-administered to the mice in doses of 100, 150 and 200mg/kgbody weight (b.w.) for acute study (6h) and subchronic study (8days). The in vivo effects of (PPc) and (SPc) EtAc extracts were assessed through blood glucose levels (BGL), changes in body weight as well as antioxidant enzyme activity. At the end of the study, animals were sacrificed for obtaining pancreatic tissues samples to assess histopathological changes in pancreas of control and alloxan-induced mice.


RESULTS
Acute administration of either PPc or SPc for 6h at all tested doses, significantly (P≤0.05) decreased BGL as compared with vehicle treated diabetic control (DIA CTRL) group with PPc showing more significant acute antidiabetic activity than those of SPc. In addition, subchronic administration of PPc has shown more significant (P≤0.05) amelioration of hyperglycemia, weight-loss and oxidative stress at all doses compared to SPc administration. Histologically, treatment with PPc and SPc showed significant pancreatic cell regeneration compared to diabetic controls.


CONCLUSIONS
Results indicated that ethyl acetate extracts of Prunus cerasus pulps and seeds promote acute and subchronic hypoglycaemic effects in alloxan-induced diabetic mice with pulp extracts having a more pronounced effect.
Department of Medical Laboratory Technology, Faculty of Health Sciences, Beirut Arab University, Lebanon. Electronic address: f.saleh@bau.edu.lb.
Fatima A
FA
Saleh
Department of Nutrition and Dietetics, Faculty of Health Sciences, Beirut Arab University, Lebanon.
Nada
N
El-Darra
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Beirut Arab University, Lebanon.
Karim
K
Raafat
D000496:Alloxan; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000473:pathology; D007004:Hypoglycemic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D007515:Islets of Langerhans / Q000187:drug effects / Q000473:pathology; D008297:Male; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D000068220:Prunus avium / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*
D000975:Antioxidants; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D000496:Alloxan