Self-assemble nanoparticles based on polypeptides containing C-terminal luminescent Pt-cysteine complex.
The growing attention to the luminescent nanocarriers is strongly stimulated by their potential application as drug delivery systems and by the necessity to monitor their distribution in cells and tissues. In this communication we report on the synthesis of amphiphilic polypeptides bearing C-terminal phosphorescent label together with preparation of nanoparticles using the polypeptides obtained. The approach suggested is based on a unique and highly technological process where the new phosphorescent Pt-cysteine complex serves as initiator of the ring-opening polymerization of α-amino acid N-carboxyanhydrides to obtain the polypeptides bearing intact the platinum chromophore covalently bound to the polymer chain. It was established that the luminescent label retains unchanged its emission characteristics not only in the polypeptides but also in more complicated nanoaggregates such as the polymer derived amphiphilic block-copolymers and self-assembled nanoparticles. The phosphorescent nanoparticles display no cytotoxicity and hemolytic activity in the tested range of concentrations and easily internalize into living cells that makes possible in vivo cell visualization, including prospective application in time resolved imaging and drug delivery monitoring.
Saint-Petersburg State University, Institute of Chemistry, Universitetsky pr. 26, 198504 St. Petersburg, Russia.
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EG
Vlakh
Saint-Petersburg State University, Institute of Chemistry, Universitetsky pr. 26, 198504 St. Petersburg, Russia.
E V
EV
Grachova
Saint-Petersburg State University, Institute of Chemistry, Universitetsky pr. 26, 198504 St. Petersburg, Russia.
D D
DD
Zhukovsky
Saint-Petersburg State University, Institute of Chemistry, Universitetsky pr. 26, 198504 St. Petersburg, Russia.
A V
AV
Hubina
Saint-Petersburg State University, Institute of Chemistry, Universitetsky pr. 26, 198504 St. Petersburg, Russia.
A S
AS
Mikhailova
Saint-Petersburg State University, Institute of Chemistry, Universitetsky pr. 26, 198504 St. Petersburg, Russia.
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JR
Shakirova
Saint-Petersburg State University, Institute of Chemistry, Universitetsky pr. 26, 198504 St. Petersburg, Russia.
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VV
Sharoyko
Saint-Petersburg State University, Institute of Chemistry, Universitetsky pr. 26, 198504 St. Petersburg, Russia.
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SP
Tunik
Saint-Petersburg State University, Institute of Chemistry, Universitetsky pr. 26, 198504 St. Petersburg, Russia.
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TB
Tennikova
D045744:Cell Line, Tumor; D003545:Cysteine / Q000737:chemistry; D057809:HEK293 Cells; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D049408:Luminescent Agents / Q000138:chemical synthesis / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000009:adverse effects / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry; D058105:Polymerization; D013501:Surface-Active Agents / Q000138:chemical synthesis / Q000737:chemistry
D049408:Luminescent Agents; D010455:Peptides; D013501:Surface-Active Agents; D010984:Platinum; D003545:Cysteine