Intracellular detection of hazardous Cd2+through a fluorescence imaging technique by using a nontoxic coumarin based sensor.
A new coumarin based turn on fluorescent sensor (R1) was reported for the detection of highly hazardous Cd2+with excellent selectivity and sensitivity without any interference of other metal ions. The single crystal X-ray structure analysis of the sensor showed the actual geometry of the molecule. For the first time, a Cd2+induced FRET mechanism was observed and explained accordingly. Instant naked eye detection of Cd2+through a sharp colour change signified the practical applicability of R1. The sensor of high quantum yield was applied in the intracellular detection of poisonous Cd2+in living HeLa S3 cells.
Department of Applied Chemistry, Indian Institute of Technology (ISM), Dhanbad-826004, India. dey.s.ac@ismdhanbad.ac.in.
Chanda
C
Kumari
Department of Applied Chemistry, Indian Institute of Technology (ISM), Dhanbad-826004, India. dey.s.ac@ismdhanbad.ac.in and Department of Chemistry, Indian Institute of Engineering Science and Technology, Shibpur, Howrah, West Bengal-711103, India.
Dibyendu
D
Sain
Department of Applied Chemistry, Indian Institute of Technology (ISM), Dhanbad-826004, India. dey.s.ac@ismdhanbad.ac.in.
Ashish
A
Kumar
Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700064, India.
Sushanta
S
Debnath
Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700064, India.
Partha
P
Saha
Department of Applied Chemistry, Indian Institute of Technology (ISM), Dhanbad-826004, India. dey.s.ac@ismdhanbad.ac.in.
Swapan
http://orcid.org/0000-0002-6168-5627
S
Dey