Albumin coatings by alternating current electrophoretic deposition for improving corrosion resistance and bioactivity of titanium implants.
Although Ti alloys are generally regarded to be highly corrosion resistant, inflammatory conditions following surgery can instigate breakdown of the TiO2passivation layer leading to an increased metal ion release. Furthermore proteins present in the surrounding tissue will readily adsorb on a titanium surface after implantation. In this paper alternating current electrophoretic deposition (AC-EPD) of bovine serum albumin (BSA) on Ti6Al4V was investigated in order to increase the corrosion resistance and control the protein adsorption capability of the implant surface. The Ti6Al4V surface was characterized with SEM, XPS and ToF-SIMS after long-term immersion tests under physiological conditions and simulated inflammatory conditions either in Dulbecco's Modified Eagle Medium (DMEM) or DMEM supplemented with fetal calf serum (FCS). The analysis showed an increased adsorption of amino acids and proteins from the different immersion solutions. The BSA coating was shown to prevent selective dissolution of the vanadium (V) rich β-phase, thus effectively limiting metal ion release to the environment. Electrochemical impedance spectroscopy measurements confirmed an increase of the corrosion resistance for BSA coated surfaces as a function of immersion time due to the time-dependent adsorption of the different amino acids (from DMEM) and proteins (from FCS) as observed by ToF-SIMS analysis.
Institute for Surface Science and Corrosion, Dept. of Mat. Science, University of Erlangen-Nürnberg, 91058 Erlangen, Germany.. Electronic address: sarah.hoehn@fau.de.
Sarah
S
Höhn
KU Leuven Department of Materials Engineering, Kasteelpark Arenberg 44, Box 2450, 3001 Leuven, Belgium. Electronic address: annabel.braem@kuleuven.be.
Annabel
A
Braem
KU Leuven Department of Materials Engineering, Kasteelpark Arenberg 44, Box 2450, 3001 Leuven, Belgium. Electronic address: bram.neirinck@3DSystems.com.
Bram
B
Neirinck
Institute for Surface Science and Corrosion, Dept. of Mat. Science, University of Erlangen-Nürnberg, 91058 Erlangen, Germany.. Electronic address: virtanen@ww.uni-erlangen.de.
Sannakaisa
S
Virtanen
D000497:Alloys; D000818:Animals; D002118:Calcium / Q000032:analysis; D002417:Cattle; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D003343:Corrosion; D058266:Dielectric Spectroscopy; D004560:Electricity; D004586:Electrophoresis; D053758:Nanoparticles / Q000648:ultrastructure; D010758:Phosphorus / Q000032:analysis; D056951:Photoelectron Spectroscopy; D019736:Prostheses and Implants; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D018629:Spectrometry, Mass, Secondary Ion; D014025:Titanium / Q000494:pharmacology*
D000497:Alloys; D020099:Coated Materials, Biocompatible; C031462:titanium alloy (TiAl6V4); D012710:Serum Albumin, Bovine; D010758:Phosphorus; D014025:Titanium; D002118:Calcium
Alternating current; Bovine serum albumin; DMEM; Electrophoretic deposition; Fetal calf serum; Ti6Al4V