GABA Pathway Rate-Limit Citrate Degradation in Postharvest Citrus Fruit Evidence from HB Pumelo (Citrus grandis) × Fairchild (Citrus reticulata) Hybrid Population.
Organic acids are a major index of fresh fruit marketing properties. However, the genetic effects on the organic acid level in postharvest citrus fruit still remain unknown. Here, we used the fruits of about 40 lines in a hybrid population (high-acid "HB Pumelo" × low-acid "Fairchild") to analyze the organic acid metabolism of postharvest citrus fruit. A transgressive content of titratable acid (TA) was observed, which was attributed to citrate accumulation. High- and low-acid fruits (No. 130, 168 and No. 080, 181, respectively) were chosen for further study. Gene expression analysis on citrate metabolism showed that the high accumulation of citrate could be attributed to the low activity of γ-aminobutyric acid (GABA) shunt, and was partially due to the block of tricarboxylic acid (TCA) cycle by low mitochondrial aconitase (m-ACO) expression. TA level was significantly negatively correlated with weight loss in fruits during postharvest storage, implying a close relationship between organic acid and water metabolism.
Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, PR China, College of Horticulture and Forestry Science, Huazhong Agricultural University , Wuhan 430070, People's Republic of China.
Ling
http://orcid.org/0000-0001-7593-9627
L
Sheng
Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, PR China, College of Horticulture and Forestry Science, Huazhong Agricultural University , Wuhan 430070, People's Republic of China.
Dandan
D
Shen
Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, PR China, College of Horticulture and Forestry Science, Huazhong Agricultural University , Wuhan 430070, People's Republic of China.
Wei
W
Yang
Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, PR China, College of Horticulture and Forestry Science, Huazhong Agricultural University , Wuhan 430070, People's Republic of China.
Mingfei
M
Zhang
Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, PR China, College of Horticulture and Forestry Science, Huazhong Agricultural University , Wuhan 430070, People's Republic of China.
Yunliu
Y
Zeng
Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, PR China, College of Horticulture and Forestry Science, Huazhong Agricultural University , Wuhan 430070, People's Republic of China.
Juan
J
Xu
Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, PR China, College of Horticulture and Forestry Science, Huazhong Agricultural University , Wuhan 430070, People's Republic of China.
Xiuxin
X
Deng
Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, PR China, College of Horticulture and Forestry Science, Huazhong Agricultural University , Wuhan 430070, People's Republic of China.
Yunjiang
Y
Cheng
D019343:Citric Acid / Q000378:metabolism*; D002957:Citrus / Q000235:genetics* / Q000378:metabolism*; D005638:Fruit / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D006824:Hybridization, Genetic; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D010940:Plant Proteins; D019343:Citric Acid; D005680:gamma-Aminobutyric Acid
citrate; citrus hybrid population; fruit water loss; gene expression; postharvest storage