Spectral characterization and antibacterial activity of an isolated compound from Memecylon edule leaves.
Memecylon edule Roxb. (Melastamataceae family) is a small evergreen tree reported as having ethnobotanical and pharmacological properties. The present study was aimed to investigate the spectral characterization and antibacterial activity of isolated pure compound (3β-hydroxyurs-12-en-28-oic acid (ursolic acid)) from Memecylon edule leaves by performing bioassay guided isolation method. The structure derivation of isolated compound was done by different spectral studies like UV, FT-IR, LC-MS, CHNS analysis, 1D (1H,13C and DEPT-135) and 2D-NMR (HSQC and HMBC), respectively. About 99.29% purity of the compound was found in LC analysis.1H NMR spectrum results of compound shown 48 protons appear at different shielded region and most of the protons were present in aliphatic region. Whereas,13C NMR spectral data resulted seven methyl carbons (CH3), nine methylene carbons (CH2), seven methine carbons (CH) and six non-hydrogenated carbons (C) which are characteristic of pentacyclic triterpene. The isolated pure compound was tested for its antibacterial properties against targeted human pathogens by performing agar well diffusion, MIC and MBC assays and the result exhibits better growth inhibitory effects against S. epidermidis and S. pneumoniae, with the MIC values of 1.56 and 3.15μg/ml. The outcome of this study suggests that the bioactive compound is used for development of plant based drugs in pharmaceutical industry for combating microbial mediated diseases.
Department of Biotechnology, K.S. Rangasamy College of Arts and Science (Autonomous), Tiruchengode, 637 215 Namakkal, Tamil Nadu, India.
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Srinivasan
Natural Drug Research Laboratory, Department of Biotechnology, Periyar University, Periyar Palkalai Nagar, Salem 636 011, Tamil Nadu, India. Electronic address: mdnataraj@rediffmail.com.
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Natarajan
Molecular Entomology Laboratory, Department of Biotechnology, Periyar University, Periyar Palkalai Nagar, Salem 636 011, Tamil Nadu, India.
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Shivakumar
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D035322:Melastomataceae; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal; D013057:Spectrum Analysis / Q000379:methods*; D013210:Staphylococcus / Q000187:drug effects; D014315:Triterpenes / Q000302:isolation & purification*; D000097245:Ursolic Acid
D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D014315:Triterpenes; D000097245:Ursolic Acid
Antibacterial compound; Memecylon edule; NMR spectrum; Ursolic acid