Starch-derived absorbable polysaccharide hemostat enhances bone healing via BMP-2 protein.
Surgical hemostasis is critical in reducing the likelihood of excessive bleeding and blood transfusion. In treating some cases, commonly used hemostatic agent showed limited efficacy and prolonged degradation and clearance, causing an inhibition of bone healing. Starch absorbable polysaccharide (SAPH) is a novel hemostatic agent made from a plant starch, which can be completely absorbed and achieve better hemostatic effects than many commonly used hemostatic agents. However, whether SAPH can induce a promotion of bone healing remains unknown. In this study, we used a model of rabbit parietal bone defect and a mouse osteoblast cell line MC3T3-E1 to evaluate the effects of SAPH on bone healing. We found that SAPH significantly decreased bone healing scores, reduced defective area of parietal bone, and increased the areas of bone trabeculae and cavitas medullaris. In addition, SAPH enhanced MC3T3-E1 osteoblasts proliferation, up-regulated the expressions of alkaline phosphatase (ALP) and osteocalcin and increased the level of bone morphogenetic protein 2 (BMP-2) in MC3T3-E1 osteoblasts. These SAPH-induced benefits in MC3T3-E1 osteoblasts were significantly abolished by the application of BMP-2-siRNA. These findings suggested that SAPH enhances bone healing, promotes the proliferation, differentiation and maturation of osteoblast by up-regulating BMP-2 expression in osteoblastic cells.
Department of Pathology and Pathophysiology, The Fourth Military Medical University, Xi'an 710032, China.
Wen
W
Niu
Department of Pathology and Pathophysiology, The Fourth Military Medical University, Xi'an 710032, China.
Yanxia
Y
Wang
Department of Pathology and Pathophysiology, The Fourth Military Medical University, Xi'an 710032, China.
Yi
Y
Liu
Department of Pathology and Pathophysiology, The Fourth Military Medical University, Xi'an 710032, China.
Bo
B
Zhang
Department of Pathology and Pathophysiology, The Fourth Military Medical University, Xi'an 710032, China.
Manling
M
Liu
Department of Pathology and Pathophysiology, The Fourth Military Medical University, Xi'an 710032, China.
Ying
Y
Luo
Department of Pathology and Pathophysiology, The Fourth Military Medical University, Xi'an 710032, China.
Pengtao
P
Zhao
Department of Anesthesiology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China; Department of Military Outpatient, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
Yao
Y
Zhang
Department of Pathology and Pathophysiology, The Fourth Military Medical University, Xi'an 710032, China.
Hanrong
H
Wu
Department of Anesthesiology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China. Electronic address: malei0214@126.com.
Lei
L
Ma
Department of Pathology and Pathophysiology, The Fourth Military Medical University, Xi'an 710032, China. Electronic address: pathophy@fmmu.edu.cn.
Zhichao
Z
Li
D020341:Absorbable Implants / Q000592:standards; D000469:Alkaline Phosphatase / Q000235:genetics; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D006488:Hemostasis, Surgical / Q000295:instrumentation* / Q000592:standards; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D015675:Osteocalcin / Q000235:genetics; D010294:Parietal Bone / Q000187:drug effects* / Q000473:pathology; D011817:Rabbits; D013213:Starch / Q000737:chemistry / Q000494:pharmacology*
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D015675:Osteocalcin; D013213:Starch; D000469:Alkaline Phosphatase
Bone healing; Bone morphogenetic protein; Hemostatic agent; Osteoblast; Starch absorbable polysaccharide