Enhanced biosafety of silica coated gadolinium based nanoparticles.
One of the most important and novel approaches of biomedical engineering is the development of new, effective and non-invasive medical diagnosis abilities, and treatments that have such requirements as advanced technologies for tumor imaging. Gadolinium (Gd) compounds can be used as MRI contrast agents, however the release of Gd3+ions presents some adverse side effects such as renal failure, pancreatitis or local necrosis. The main aim of the work was the development and optimization of Gadolinium based nanoparticles coated with silica to be used as bioimaging agent. Gd based nanoparticles were prepared through a precipitation method and afterwards, these nanoparticles were covered with silica, using Stöber method with ammonia and functionalized with 3-Aminopropyltriethoxysilane (APTES). Results showed that nanoparticles were homogeneous regarding chemical composition, silica layer thickness, total size and morphology. Also, silica coating was successfully not degraded after 4 weeks at pH 5.5, 6.0 and 7.4, contrary to GdOHCO3nanoparticles that degraded. Regarding the in vitro cell tests, very good cell proliferation and viability were observed. In conclusion, the results showed that Gd based nanoparticles coated with silica for imaging applications were successfully obtained under a well-controlled method. Furthermore, silica coating may enhance magnetic nanoparticles biosafety because it avoids GdOHCO3degradation into harmful products (such as Gd3+ions) at physiological conditions.
i3S, Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal. msl@ineb.up.pt.|INEB, Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal. msl@ineb.up.pt.|Faculdade de Engenharia, DEMM, Universidade do Porto, Porto, Portugal. msl@ineb.up.pt.
Marta
M
Laranjeira
Frontier Research Academy for Young Researchers, Kitakyushu-shi, Fukuoka, Japan.
Yuki
Y
Shirosaki
Frontier Research Academy for Young Researchers, Kitakyushu-shi, Fukuoka, Japan.
Saki
S
Yoshimatsu Yasutomi
Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Kyushu, Fukuoka, Japan.
Toshiki
T
Miyazaki
i3S, Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.|INEB, Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal.|Faculdade de Engenharia, DEMM, Universidade do Porto, Porto, Portugal.
Fernando Jorge
FJ
Monteiro
D001698:Biomedical Engineering; D049109:Cell Proliferation; D002470:Cell Survival; D003287:Contrast Media / Q000737:chemistry; D005347:Fibroblasts / Q000378:metabolism; D005682:Gadolinium / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology / Q000379:methods*; D009336:Necrosis / Q000188:drug therapy; D010195:Pancreatitis / Q000188:drug therapy; D011437:Propylamines / Q000737:chemistry; D051437:Renal Insufficiency / Q000188:drug therapy; D012821:Silanes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014961:X-Ray Diffraction
D003287:Contrast Media; D058185:Magnetite Nanoparticles; D011437:Propylamines; D012821:Silanes; D012822:Silicon Dioxide; D005682:Gadolinium; D007770:L-Lactate Dehydrogenase; C477625:amino-propyl-triethoxysilane