A heparin-functionalized carbon nanotube-based affinity biosensor for dengue virus.
Dengue virus is an arthropod-borne virus transmitted primarily by Aedes mosquitos and is major cause of disease in tropical and subtropical regions. Colloquially known as Dengue Fever, infection can cause hemorrhagic disorders and death in humans and non-human primates. We report a novel electronic biosensor based on a single-walled carbon nanotube network chemiresistive transducer that is functionalized with heparin for low-cost, label-free, ultra-sensitive, and rapid detection of whole dengue virus (DENV). Heparin, an analog of the heparan sulfate proteoglycans that are receptors for dengue virus during infection of Vero cells and hepatocytes, was used for the first time in a biosensor as a biorecognition element instead of traditional antibody. Detection of DENV in viral culture supernatant has similar sensitivity as the corresponding viral titer in phosphate buffer despite the presence of growth media and Vero cell lysate. The biosensor demonstrated sensitivity within the clinically relevant range for humans and infected Aedes aegypti. It has potential application in clinical diagnosis and can improve point-of-care diagnostics of dengue infection.
Department of Environmental Sciences, University of California, Riverside, CA 92521, USA.
Daniel
D
Wasik
Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USA. Electronic address: adani@engr.ucr.edu.
Ashok
A
Mulchandani
Department of Environmental Sciences, University of California, Riverside, CA 92521, USA.
Marylynn V
MV
Yates
D000330:Aedes / Q000821:virology; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D002522:Chlorocebus aethiops; D003715:Dengue / Q000175:diagnosis / Q000821:virology*; D003716:Dengue Virus / Q000302:isolation & purification*; D004867:Equipment Design; D019812:Heparan Sulfate Proteoglycans / Q000737:chemistry; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D014709:Vero Cells
D019812:Heparan Sulfate Proteoglycans; D037742:Nanotubes, Carbon; D006493:Heparin
Carbon nanotubes; Chemiresistor; Dengue virus; Label-free biosensor; Virus detection