Progress in biocatalysis with immobilized viable whole cells: systems development, reaction engineering and applications.
Viable microbial cells are important biocatalysts in the production of fine chemicals and biofuels, in environmental applications and also in emerging applications such as biosensors or medicine. Their increasing significance is driven mainly by the intensive development of high performance recombinant strains supplying multienzyme cascade reaction pathways, and by advances in preservation of the native state and stability of whole-cell biocatalysts throughout their application. In many cases, the stability and performance of whole-cell biocatalysts can be highly improved by controlled immobilization techniques. This review summarizes the current progress in the development of immobilized whole-cell biocatalysts, the immobilization methods as well as in the bioreaction engineering aspects and economical aspects of their biocatalytic applications.
Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak Technical University, Bratislava, Slovakia.
Milan
M
Polakovič
Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak Technical University, Bratislava, Slovakia.
Juraj
J
Švitel
Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia.
Marek
M
Bučko
Center for Advanced Materials, Qatar University, Doha, Qatar.
Jaroslav
J
Filip
Institute of Scientific Instruments, Academy of Sciences Czech Republic, Brno, Czech Republic.
Vilém
V
Neděla
Institute for Microbiology, Faculty of Science, Technische Universität Dresden, Dresden, Germany.
Marion B
MB
Ansorge-Schumacher
Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia. Peter.Gemeiner@savba.sk.
Peter
P
Gemeiner
D000818:Animals; D055162:Biocatalysis; D057005:Bioengineering; D019149:Bioreactors; D018914:Cells, Immobilized; D006801:Humans
Biocatalysis; Immobilization methods; Immobilized whole-cell biocatalyst; Multienzyme cascade reactions; Process economics; Reaction engineering