The role of β-arrestin-2 on Fear/anxious-related memory in a rat model of Post-traumatic stress disorder.
Post-traumatic stress disorder (PTSD) can be categorised as a disorder of dysregulated fear processing. In the formation and development of PTSD, whether fear/anxious-related memory is regulated by β-arrestin-2, and happened along the signal transduction pathways remains unknown.



We used single prolonged stress (SPS) as the animal model of PTSD. Next, elevated plus maze tests (EPM) was performed to examine fear/anxious memory- related behaviors. Then, we detected β-arrestin-2, PDE-4, and signal transduction pathways with immunoﬂuorescence, co-immunoprecipitation, immunohistochemistry, Elisa, western blot, RT-PCR, and real-time PCR.



Our data indicated that SPS caused fear/anxious memory-related behaviors enhancement. The low expression of β-arrestin-2, PDE-4 and their complex on SPS 7d, and high expression of signal transduction pathways on SPS7d in basolateral amygdala (BLA).



That indicating that β-arrestin-2 is critical for the formation of abnormal fear/anxious memory in PTSD; and fear/anxious memory occured through signal transduction pathways. Finally, these results suggest that β-arrestin-2, PDE-4 and signal transduction pathways may be by influencing the fear/anxious memory thereby involved in the formation and development of PTSD.
PTSD Lab, Department of Histology and Embryology, Institute of Pathology and Pathophysiology, Basic Medical College, China Medical University, Shenyang, PR China; Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands.
Jinlan
J
Ding
PTSD Lab, Department of Histology and Embryology, Institute of Pathology and Pathophysiology, Basic Medical College, China Medical University, Shenyang, PR China.
Fang
F
Han
PTSD Lab, Department of Histology and Embryology, Institute of Pathology and Pathophysiology, Basic Medical College, China Medical University, Shenyang, PR China.
Lili
L
Wen
PTSD Lab, Department of Histology and Embryology, Institute of Pathology and Pathophysiology, Basic Medical College, China Medical University, Shenyang, PR China.
Bing
B
Xiao
PTSD Lab, Department of Histology and Embryology, Institute of Pathology and Pathophysiology, Basic Medical College, China Medical University, Shenyang, PR China. Electronic address: shiyuxiu@163.com.
Yuxiu
Y
Shi
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism; D000679:Amygdala / Q000378:metabolism*; D000818:Animals; D001007:Anxiety / Q000378:metabolism; D001522:Behavior, Animal / Q000502:physiology; D000242:Cyclic AMP / Q000378:metabolism; D004195:Disease Models, Animal; D005239:Fear / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D008568:Memory / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D013313:Stress Disorders, Post-Traumatic / Q000378:metabolism* / Q000523:psychology; D000071560:beta-Arrestin 2 / Q000378:metabolism*
D000071560:beta-Arrestin 2; D000242:Cyclic AMP; D015105:3',5'-Cyclic-AMP Phosphodiesterases
Basolateral amygdala; Post- traumatic stress disorder; β-arrestin-2