Impact of resistance training on the autophagy-inflammation-apoptosis crosstalk in elderly subjects.
Aging is associated with a decline in autophagy and a state of low-grade inflammation which further affects apoptosis and autophagy. Importantly, these alterations could reverse with regular physical activity. This study assessed the effects of a resistance exercise training program on autophagy, NLRP3 inflammasome, and apoptosis in peripheral blood mononuclear cells (PBMCs) from old subjects. Twenty-six healthy women and men (age, 69.6±1.5 yr) were randomized to a training (TG) or a control (CG) group. TG performed an 8-week resistance training program, while CG followed their daily routines. Protein expression of beclin-1, Atg12, Atg16 and LAMP-2 increased following the training program, while expression of p62/SQSTM1 and phosphorylation of ULK-1 at Ser757 were significantly lower. Resistance exercise also induced a decrease in NLRP3 expression and in the caspase-1/procaspase-1 ratio. Expression of Bcl-2 and Bcl-xL, as well as the Bad/BcL-2 ratio were reduced, and there was a significant decrease in the protein content of caspase-3. The results obtained seem to indicate that 8-week resistance training stimulates autophagy, prevents NLRP3 inflammasome activation, and reduces apoptosis in PBMCs from elderly subjects. These data could have a significant impact in prevention and rehabilitation programs currently employed in elderly population.
Institute of Biomedicine (IBIOMED), University of León, León, Spain.
Yubisay
Y
Mejías-Peña
Institute of Biomedicine (IBIOMED), University of León, León, Spain.
Brisamar
B
Estébanez
Division of Clinical Translational Science, Georgia Prevention Institute, Department of Pediatrics, Augusta University, Augusta, GA, USA.
Paula
P
Rodriguez-Miguelez
Radiobiology Unit, Laboratory of Molecular and Cellular Biology, Institute for Environment, Health and Safety, Belgian Nuclear Research Centre (SCK•CEN), Mol, Belgium.
Rodrigo
R
Fernandez-Gonzalo
Institute of Biomedicine (IBIOMED), University of León, León, Spain.
Mar
M
Almar
Institute of Biomedicine (IBIOMED), University of León, León, Spain.
José A
JA
de Paz
Institute of Biomedicine (IBIOMED), University of León, León, Spain.
Javier
J
González-Gallego
Institute of Biomedicine (IBIOMED), University of León, León, Spain.
María J
MJ
Cuevas
D000368:Aged; D017209:Apoptosis / Q000502:physiology*; D001343:Autophagy / Q000502:physiology*; D000071192:Autophagy-Related Protein 12 / Q000378:metabolism; D000071183:Autophagy-Related Proteins / Q000378:metabolism; D000071186:Beclin-1 / Q000378:metabolism; D005260:Female; D006801:Humans; D058847:Inflammasomes / Q000378:metabolism*; D007249:Inflammation / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D052119:Lysosomal-Associated Membrane Protein 2 / Q000378:metabolism; D008297:Male; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein / Q000378:metabolism; D055070:Resistance Training
C466624:ATG12 protein, human; C518959:ATG16L1 protein, human; D000071192:Autophagy-Related Protein 12; D000071183:Autophagy-Related Proteins; D000071186:Beclin-1; D058847:Inflammasomes; C497811:LAMP2 protein, human; D052119:Lysosomal-Associated Membrane Protein 2; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein
apoptosis; autophagy; elderly; inflammasome; resistance exercise