Improvement of osteogenesis in dental pulp pluripotent-like stem cells by oligopeptide-modified poly(β-amino ester)s.
Controlling pluripotent stem cell differentiation via genetic manipulation is a promising technique in regenerative medicine. However, the lack of safe and efficient delivery vehicles limits this application. Recently, a new family of poly(β-amino ester)s (pBAEs) with oligopeptide-modified termini showing high transfection efficiency of both siRNA and DNA plasmid has been developed. In this study, oligopeptide-modified pBAEs were used to simultaneously deliver anti-OCT3/4 siRNA, anti-NANOG siRNA, and RUNX2 plasmid to cells from the dental pulp with pluripotent-like characteristics (DPPSC) in order to promote their osteogenic differentiation. Results indicate that transient inhibition of the pluripotency marker OCT3/4 and the overexpression of RUNX2 at day 7 of differentiation markedly increased and accelerated the expression of osteogenic markers. Furthermore, terminally-differentiated cells exhibited higher matrix mineralization and alkaline phosphatase activity. Finally, cell viability and genetic stability assays indicate that this co-delivery system has high chromosomal stability and minimal cytotoxicity. Therefore, we conclude that such co-delivery strategy is a safe and a quick option for the improvement of DPPSC osteogenic differentiation.



Controlling pluripotent stem cell differentiation via genetic manipulation is a promising technique in regenerative medicine. However, the lack of safe and efficient delivery vehicles limits this application. In this study, we propose the use of a new family of oligopeptide-modified pBAEs developed in our group to control the differentiation of dental pulp pluripotential stem cells (DPPSC). In order to promote their osteogenic differentiation. The strategy proposed markedly increased and accelerated the expression of osteogenic markers, cell mineralization and alkaline phosphatase activity. Finally, cell viability and genetic stability assays indicated that this co-delivery system has high chromosomal stability and minimal cytotoxicity. These findings open a new interesting path in the usage of non-viral gene delivery systems for the control of pluripotential stem cell differentiation.
Regenerative Medicine Research Institute, Universitat Internacional de Catalunya, Barcelona, Spain.
Raquel
R
Núñez-Toldrà
Grup d'Enginyeria de Materials (GEMAT), Institut Químic de Sarrià, Universitat Ramon Llull, Barcelona, Spain.
Pere
P
Dosta
Regenerative Medicine Research Institute, Universitat Internacional de Catalunya, Barcelona, Spain.
Sheyla
S
Montori
Grup d'Enginyeria de Materials (GEMAT), Institut Químic de Sarrià, Universitat Ramon Llull, Barcelona, Spain. Electronic address: victor.ramos@iqs.url.edu.
Víctor
V
Ramos
Regenerative Medicine Research Institute, Universitat Internacional de Catalunya, Barcelona, Spain. Electronic address: matari@uic.es.
Maher
M
Atari
Grup d'Enginyeria de Materials (GEMAT), Institut Químic de Sarrià, Universitat Ramon Llull, Barcelona, Spain; Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Zaragoza, Spain.
Salvador
S
Borrós
D001672:Biocompatible Materials / Q000737:chemistry; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000037:antagonists & inhibitors / Q000235:genetics; D003782:Dental Pulp / Q000166:cytology*; D016503:Drug Delivery Systems; D042822:Genomic Instability; D006801:Humans; D008422:Materials Testing; D000071317:Nanog Homeobox Protein / Q000037:antagonists & inhibitors / Q000235:genetics; D050814:Octamer Transcription Factor-3 / Q000037:antagonists & inhibitors / Q000235:genetics; D009842:Oligopeptides / Q000737:chemistry; D010012:Osteogenesis / Q000235:genetics / Q000502:physiology*; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000502:physiology*; D011108:Polymers / Q000737:chemistry; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics; D014162:Transfection
D001672:Biocompatible Materials; D050659:Core Binding Factor Alpha 1 Subunit; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; D009842:Oligopeptides; C494173:POU5F1 protein, human; D011108:Polymers; D034741:RNA, Small Interfering; C493709:RUNX2 protein, human; C507253:poly(beta-amino ester)
DPPSC; Dental pulp; Genetic stability; Non-viral systems; Osteogenic differentiation; Pluripotency; Poly(β-amino ester)