In vitro biocompatibility of a ferrimagnetic glass-ceramic for hyperthermia application.
Ferrimagnetic glass-ceramics containing magnetite crystals were developed for hyperthermia applications of solid neoplastic tissue. The present work is focused on in vitro evaluation of the biocompatibility of these materials, before and after soaking in a simulated body fluid (SBF). X-ray diffraction, scanning electron microscopy, atomic absorption spectrophotometry, X-ray photoelectron spectrometry and pH measurements were employed in glass-ceramic characterisation. The free-radical mediated reactivity of the glass-ceramic was evaluated by Electron Paramagnetic Resonance (EPR) spin trapping. Cell adhesion and proliferation tests were carried out by using 3T3 murine fibroblasts. Cytotoxicity was performed by qualitative evaluation of human bone osteosarcoma cells U2OS cell line. The results show that almost two times more 3T3 cells proliferated on the samples pre-treated in SBF, compared with the untreated specimens. Moreover a decrease of confluence was observed at 48 and 72h for U2OS cells exposed to the untreated glass-ceramic, while the powder suspensions of glass-ceramic pre-treated in SBF did not influence the cell morphology up to 72h of exposition. The untreated glass-ceramic exhibited Fenton-like reactivity, as well as reactivity towards formate molecule. After pre-treatment with SBF the reactivity towards formate was completely suppressed. The concentration of iron released into the SBF solution was below 0.1ppm at 37°C, during one month of soaking. The different in vitro behaviour of the samples before and after SBF treatment has been correlated to the bioactive glass-ceramic surface modifications as detected by morphological, structural and compositional analyses.
Applied Science and Technology Department, Institute of Materials Physics and Engineering, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Torino, Italy.
Oana
O
Bretcanu
Applied Science and Technology Department, Institute of Materials Physics and Engineering, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Torino, Italy.
Marta
M
Miola
Department of Chemistry, University of Milan, Via Golgi 19, 20133 Milan, Italy.
Claudia L
CL
Bianchi
Department of Experimental Oncology, European Institute of Oncology, Milan, Italy.
Ida
I
Marangi
Department of Experimental Oncology, European Institute of Oncology, Milan, Italy.
Roberta
R
Carbone
Department of Chemistry, University of Torino, Via Pietro Giuria 7, 10125 Torino, Italy; "G. Scansetti" Interdepartmental Centre for Studies on Asbestos and other Toxic Particulates, Via Pietro Giuria 7, 10125 Torino, Italy.
Ingrid
I
Corazzari
Department of Medical Science, Human Anatomy, University of Eastern Piedmont, Novara, Italy.
Mario
M
Cannas
Applied Science and Technology Department, Institute of Materials Physics and Engineering, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Torino, Italy. Electronic address: enrica.verne@polito.it.
Enrica
E
Verné
D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D002516:Ceramics / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006979:Hyperthermia, Induced / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D059346:Magnets / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D056951:Photoelectron Spectroscopy; D012996:Solutions; D013054:Spectrophotometry, Atomic; D014961:X-Ray Diffraction
D001672:Biocompatible Materials; D012996:Solutions; C023506:Glass ceramics
Cancer; Cell culture; Ferrimagnetic; Glass-ceramic; Hyperthermia; Magnetite