Antifungal Activity of Lactic Acid Bacteria Strains Isolated from Natural Honey against PathogenicCandidaSpecies.
The role of lactic acid bacteria (LAB) in honey as antifungal activity has received little attention and their mechanism of inhibitory of fungi is not fully understood. In this study, LAB were isolated from honey samples from Malaysia, Libya, Saudi Arabia, and Yemen. Twenty-five isolates were confirmed LAB by catalase test and Gram staining, and were screened for antifungal activity. Four LAB showed inhibitory activity againstCandidaspp. using the dual agar overlay method. And they were identified asLactobacillus plantarumHS isolated from Al-Seder honey,Lactobacillus curvatusHH isolated from Al-Hanon honey,Pediococcus acidilacticiHC isolated from Tualang honey andPediococcus pentosaceusHM isolated from Al-Maray honey by the 16S rDNA sequence. The growth ofCandida glabrataATCC 2001 was strongly inhibited (>15.0 mm) and (10~15 mm) by the isolates ofL. curvatusHH andP. pentosaceusHM, respectively. The antifungal activity of the crude supernatant (cell free supernatant, CFS) was evaluated using well diffusion method. The CFS showed high antifungal activity againstCandidaspp. especially The CFS ofL. curvatusHH was significantly (p< 0.05) inhibited growth ofC. glabrataATCC 2001,C. parapsilosisATCC 2201, andC. tropicalisATCC 750 with inhibitory zone 22.0, 15.6, and 14.7 mm, respectively. While CFS ofP. pentosaceusHM was significantly (p< 0.05) effective againstC. krusei,C. glabrata, andC. albicanswith inhibition zone 17.2, 16.0, and 13.3 mm, respectively. The results indicated that LAB isolated from honey produced compounds which can be used to inhibit the growth of the pathogenicCandidaspecies.
Department of Microbiology, Faculty of Science and Technology, Universiti Sains Islam Malaysia (USIM), 71800 Nilai, Malaysia.
Bulgasem Y
BY
Bulgasem
School of Food Science and Technology, Universiti Malaysia Terengganu (UMT), 21030 Kuala Terengganu, Malaysia.
Mohd Nizam
MN
Lani
Department of Microbiology, Faculty of Science and Technology, Universiti Sains Islam Malaysia (USIM), 71800 Nilai, Malaysia.
Zaiton
Z
Hassan
School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Malaysia.
Wan Mohtar
WM
Wan Yusoff
Department of Community Health, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia.
Sumaya G
SG
Fnaish
Antifungal activity; Honey; Lactic acid bacteria; Pathogenic Candida species