Viral Effects of a dsRNA Mycovirus (PoV-ASI2792) on the Vegetative Growth of the Edible MushroomPleurotus ostreatus.
A double-stranded RNA (dsRNA) mycovirus was detected in malformed fruiting bodies ofPleurotus ostreatusstrain ASI2792, one of bottle cultivated commercial strains of the edible oyster mushroom. The partial RNA-dependent RNA polymerase (RdRp) gene of theP. ostreatusASI2792 mycovirus (PoV-ASI2792) was cloned, and a cDNA sequences alignment revealed that the sequence was identical to the RdRp gene of a known PoSV found in theP. ostreatusstrain. To investigate the symptoms of PoV-ASI2792 infection by comparing the isogenic virus-freeP. ostreatusstrains with a virus-infected strain, isogenic virus-curedP. ostreatusstrains were obtained by the mycelial fragmentation method for virus curing. The absence of virus was verified with gel electrophoresis after dsRNA-specific virus purification and Northern blot analysis using a partial RdRp cDNA of PoV-ASI2792. The growth rate and mycelial dry weight of virus-infectedP. ostreatusstrain with PoV-ASI2792 mycovirus were compared to those of three virus-free isogenic strains on 10 different media. The virus-cured strains showed distinctly higher mycelial growth rates and dry weights on all kinds of experimental culture media, with at least a 2.2-fold higher mycelial growth rate on mushroom complete media (MCM) and Hamada media, and a 2.7-fold higher mycelial dry weight on MCM and yeastmalt-glucose agar media than those of the virus-infected strain. These results suggest that the infection of PoV mycovirus has a deleterious effect on the vegetative growth ofP. ostreatus.
Department of Bio-Environmental Chemistry, Institute of Life Science and Natural Resources, Wonkwang University, Iksan 54538, Korea.
Ha-Yeon
HY
Song
Department of Bio-Environmental Chemistry, Institute of Life Science and Natural Resources, Wonkwang University, Iksan 54538, Korea.
Hyo-Jin
HJ
Choi
Department of Bio-Environmental Chemistry, Institute of Life Science and Natural Resources, Wonkwang University, Iksan 54538, Korea.
Hansaem
H
Jeong
Department of Bio-Environmental Chemistry, Institute of Life Science and Natural Resources, Wonkwang University, Iksan 54538, Korea.
Dahye
D
Choi
Institute for Molecular Biology and Genetics, Center for Fungal Pathogenesis, Chonbuk National University, Jeonju 54896, Korea.
Dae-Hyuk
DH
Kim
Department of Bio-Environmental Chemistry, Institute of Life Science and Natural Resources, Wonkwang University, Iksan 54538, Korea.
Jung-Mi
JM
Kim
Curing; Isogenic strain; Mycovirus; Pleurotus ostreatus; Viral symptom