Protective effects of essential oil of Citrus limon against aspirin-induced toxicity in IEC-6 cells.
Aspirin, one of the widely used nonsteroidal anti-inflammatory drugs, is the most highly consumed pharmaceutical product in the world. However, it has several side effects in cells. This study was designed to investigate the antioxidative activity and cytoprotective effects of essential oil of Citrus limon (EOC) extracted from leaves against aspirin-induced damages in the rat small intestine epithelial cells (IEC-6). Biochemical indicators were used to assess cytotoxicity and oxidative damages caused by aspirin treatment on IEC-6. Our results showed that the chemical characterization of EOC identified 25 compounds representing 98.19% of the total oil. The major compounds from this oil were z-citral (53.21%), neryl acetate (13.06%), geranyl acetate (10.33%), and limonene (4.23%). Aspirin induced a decrease in cell viability as well as an increase in superoxide dismutase (SOD) and catalase (CAT) activities. Contrariwise, the co-exposure of cells to aspirin and EOC alleviated every above syndrome by an increase in cell survival and decrease in SOD and CAT activities. In conclusion, the essential oil of C. limon has a potent cytoprotective effect against aspirin-induced toxicity in IEC-6 cells.
a Physiology Department-EA1274, Faculty of Medicine and Health Sciences, University of Western Brittany, 22 Avenue Camille Desmoulins, CS 93837, 29238 Brest Cedex 3, France.|b Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences Sfax, Road Soukra km 3.5, PB no 171-3000, Sfax-Tunisia.|c Laboratory of Animal Eco Physiology, Faculty of Sciences of Gafsa, 2112, Tunisia.
Hafsia
H
Bouzenna
b Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences Sfax, Road Soukra km 3.5, PB no 171-3000, Sfax-Tunisia.|c Laboratory of Animal Eco Physiology, Faculty of Sciences of Gafsa, 2112, Tunisia.
Najla
N
Hfaiedh
a Physiology Department-EA1274, Faculty of Medicine and Health Sciences, University of Western Brittany, 22 Avenue Camille Desmoulins, CS 93837, 29238 Brest Cedex 3, France.
Marie-Agnès
MA
Giroux-Metges
b Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences Sfax, Road Soukra km 3.5, PB no 171-3000, Sfax-Tunisia.
Abdelfattah
A
Elfeki
a Physiology Department-EA1274, Faculty of Medicine and Health Sciences, University of Western Brittany, 22 Avenue Camille Desmoulins, CS 93837, 29238 Brest Cedex 3, France.
Hélène
H
Talarmin
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000633:toxicity; D001241:Aspirin / Q000633:toxicity*; D001713:Biphenyl Compounds / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002957:Citrus / Q000737:chemistry*; D004847:Epithelial Cells / Q000187:drug effects*; D016166:Free Radical Scavengers; D007413:Intestinal Mucosa / Q000166:cytology*; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*; D010851:Picrates / Q000633:toxicity; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology; D051381:Rats
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D009822:Oils, Volatile; D010851:Picrates; D020011:Protective Agents; C004931:1,1-diphenyl-2-picrylhydrazyl; D001241:Aspirin
Citrus limon; activités antioxydantes; antioxidant activities; aspirin; aspirine; cytotoxicity; cytotoxicité; essential oil; huile essentielle