Histochemical Demonstration of Tripeptidyl Aminopeptidase I.
Enzyme histochemical methods are valuable for the studies on the enzyme involvement in different pathological processes. Here we describe two protocols for chromogenic and fluorogenic histochemical demonstration of tripeptidyl aminopeptidase I (TPPI), a protease that is crucial for neuronal functions. The procedures are based on newly synthesized substrates for TPPI-glycyl-L-prolyl-L-metionyl-5-chloro-1-anthraquinonylhydrazide (GPM-CAH) and glycyl-L-prolyl-L-metionyl-4-hydrazido-N-hexyl-1,8-naphthalimide (GPM-HHNI). Using such protocols, precise enzyme localization can be obtained in tissue sections of mammalian organs.
Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Mashenka B
MB
Dimitrova
Institute of Neurobiology, Bulgarian Academy of Sciences, Sofia, Bulgaria.|Department of Anatomy, Faculty of Medicine, Trakia University, Stara Zagora, Bulgaria.
Dimitrinka Y
DY
Atanasova
Institute of Neurobiology, Bulgarian Academy of Sciences, Sofia, Bulgaria. nlazarov@medfac.acad.bg.|Department of Anatomy and Histology, Medical University of Sofia, 2, Zdrave Street, 1431, Sofia, Bulgaria. nlazarov@medfac.acad.bg.
Nikolai E
NE
Lazarov
D000626:Aminopeptidases / Q000378:metabolism*; D000818:Animals; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000378:metabolism*; D004789:Enzyme Activation; D005456:Fluorescent Dyes; D006651:Histocytochemistry / Q000379:methods*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D051381:Rats; D057057:Serine Proteases / Q000378:metabolism*; D013379:Substrate Specificity; D000091346:Tripeptidyl-Peptidase 1
D005456:Fluorescent Dyes; C000717268:Tpp1 protein, mouse; C000717269:Tpp1 protein, rat; D000091346:Tripeptidyl-Peptidase 1; D057057:Serine Proteases; D000626:Aminopeptidases; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases
Brightfield microscopy; Chromogenic and fluorogenic substrates; Enzyme histochemistry; Fluorescence microscopy; Mouse; Rat; Tripeptidyl peptidase I