Analysis of serum interleukin-27 and interleukin-35 concentrations in patients with Guillain-Barré syndrome.
BACKGROUND
Guillain-Barré syndrome (GBS) is a postinfectious immune-mediated peripheral neuropathy. Interleukin (IL)-27 and IL-35 have been recognized as novel members of IL-12 family. We evaluated the serum and cerebral spinal fluid (CFS) concentrations of IL-27 and IL-35 in GBS and analyze their correlations with clinical characteristics.


METHODS
Serum samples from 50 patients with GBS including 9 acute inflammatory demyelinating polyradiculoneuropathy (AIDP), 33 acute motor axonal neuropathy (AMAN) and 8 unclassified and 35 age- and sex-matched healthy controls were collected. Thirty CSF samples from these patients and 25 patients with other noninflammatory neurological disorders (ONNDs) as disease controls were collected after lumbar puncture. Serum and CSF IL-27 and IL-35 concentrations were measured using human IL-27 or IL-35 ELISA.


RESULTS
Serum IL-27 concentrations were elevated (p=0.002) whereas serum IL-35 concentrations were decreased (p=0.031) in patients with GBS comparing with healthy controls, particularly in patients exhibiting AMAN (p=0.012). Additionally, serum IL-35 concentrations were negatively correlated with disease severity and outcomes in patients with AMAN (r=-0.358, p=0.041; r=-0.416, p=0.016).


CONCLUSIONS
IL-27 might be pathogenic, whereas IL-35 be protective in GBS. Additionally, serum IL-35 concentrations may be important biomarkers for the severity and outcomes of AMAN.
Department of Neurology and Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin 300052, China.
Lin-Jie
LJ
Zhang
Department of Neurology and Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin 300052, China.
Hui-Yue
HY
Guo
Department of Neurology and Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin 300052, China.
Da-Qi
DQ
Zhang
School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
Rong
R
Wang
Department of Neurology and Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin 300052, China.
Ting
T
Li
Department of Neurology and Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin 300052, China.
Li-Min
LM
Li
Department of Rehabilitation, Tianjin Medical University General Hospital, Tianjin 300052, China.
Dong-Mei
DM
Suo
Department of Neurology and Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin 300052, China. Electronic address: yangli2001@tmu.edu.cn.
Li
L
Yang
D000328:Adult; D016022:Case-Control Studies; D005260:Female; D020275:Guillain-Barre Syndrome / Q000097:blood*; D006801:Humans; D064094:Interleukin-27 / Q000097:blood*; D007378:Interleukins / Q000097:blood*; D008297:Male
D064094:Interleukin-27; D007378:Interleukins; C532417:interleukin-35, human
Acute motor axonal neuropathy; Guillain-Barré syndrome; Interleukin-27; Interleukin-35