Photon counting phosphorescence lifetime imaging with TimepixCam.
TimepixCam is a novel fast optical imager based on an optimized silicon pixel sensor with a thin entrance window and read out by a Timepix Application Specific Integrated Circuit. The 256 × 256 pixel sensor has a time resolution of 15 ns at a sustained frame rate of 10 Hz. We used this sensor in combination with an image intensifier for wide-field time-correlated single photon counting imaging. We have characterised the photon detection capabilities of this detector system and employed it on a wide-field epifluorescence microscope to map phosphorescence decays of various iridium complexes with lifetimes of about 1 μs in 200 μm diameter polystyrene beads.
Department of Physics, King's College London, Strand, London WC2R 2LS, United Kingdom.
Liisa M
0000000286168415
LM
Hirvonen
Brookhaven National Laboratory, Upton, New York 11973, USA.
Merlin
M
Fisher-Levine
Department of Physics, King's College London, Strand, London WC2R 2LS, United Kingdom.
Klaus
K
Suhling
Brookhaven National Laboratory, Upton, New York 11973, USA.
Andrei
A
Nomerotski