Simultaneous determination of tanshinol, protocatechuic aldehyde, protocatechuic acid, notoginsenoside R1, ginsenoside Rg1 and Rb1 in rat plasma by LC-MS/MS and its application.
Dantonic pill, consisting of Salviae miltiorrhize, Panax notoginseng and Borneol, is a widely used compound Chinese medicine for preventing and treating ischemic cardiovascular diseases in China. In the present study, an original and sensitive method for simultaneous determination of tanshinol (i.e. danshensu), protocatechuic aldehyde, protocatechuic acid, notoginsenoside R1, ginsenoside Rg1 and Rb1 in rat plasma by liquid chromatography-tandem mass spectrometry operated in positive/negative ion switching mode was established and validated. The lower limits of quantification for tanshinol, protocatechuic aldehyde, protocatechuic acid, notoginsenoside R1, ginsenoside Rg1 and Rb1 were 5, 0.5, 1, 0.5, 0.5 and 2 ng/mL, respectively. All of the calibration curves showed good linearity over the investigated concentration range (r > 0.99). Validation results demonstrated that the above compounds were accurately, precisely and robustly quantified in rat plasma. The method was successfully applied to characterize the pharmacokinetic profiles of all six compounds in rats following a single oral administration of Dantonic pill.
Tasly Academy, Tasly Holding Group Co., Ltd, Tianjin, China.|State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tasly Pharmaceutical Group Co., Ltd., Tianjin, China.|Center of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, China.
Tingyang
T
Li
Tasly Academy, Tasly Holding Group Co., Ltd, Tianjin, China.|State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tasly Pharmaceutical Group Co., Ltd., Tianjin, China.
Yang
Y
Chu
Tasly Academy, Tasly Holding Group Co., Ltd, Tianjin, China.|State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tasly Pharmaceutical Group Co., Ltd., Tianjin, China.|College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
Kaijing
K
Yan
Tasly Academy, Tasly Holding Group Co., Ltd, Tianjin, China.|State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tasly Pharmaceutical Group Co., Ltd., Tianjin, China.
Shuming
S
Li
Tasly Academy, Tasly Holding Group Co., Ltd, Tianjin, China.|State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tasly Pharmaceutical Group Co., Ltd., Tianjin, China.
Xiangyang
X
Wang
Tasly Academy, Tasly Holding Group Co., Ltd, Tianjin, China.|State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tasly Pharmaceutical Group Co., Ltd., Tianjin, China.|Center of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, China.
Ying
Y
Wang
Tasly Academy, Tasly Holding Group Co., Ltd, Tianjin, China.|State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tasly Pharmaceutical Group Co., Ltd., Tianjin, China.|National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
Wei
W
Li
Tasly Academy, Tasly Holding Group Co., Ltd, Tianjin, China.|State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tasly Pharmaceutical Group Co., Ltd., Tianjin, China.
Xiaohui
X
Ma
Center of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, China.
Jin
J
Yang
State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tasly Pharmaceutical Group Co., Ltd., Tianjin, China.|The State Key Laboratory of Drug Delivery Technology and Pharmacokinetics, Tianjin Institute of Pharmaceutical Research, Tianjin, China.
Changxiao
C
Liu
D000818:Animals; D001547:Benzaldehydes / Q000097:blood*; D002109:Caffeic Acids / Q000097:blood*; D002138:Calibration; D002396:Catechols / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D036145:Ginsenosides / Q000097:blood*; D062385:Hydroxybenzoates / Q000097:blood*; D057230:Limit of Detection; D051381:Rats; D012015:Reference Standards; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
D001547:Benzaldehydes; D002109:Caffeic Acids; D002396:Catechols; D036145:Ginsenosides; D062385:Hydroxybenzoates; C585969:tanshinol; C009091:protocatechuic acid; C005581:protocatechualdehyde; C035054:ginsenoside Rg1; C072936:notoginsenoside R1
Dantonic pill; LC-MS/MS; pharmacokinetics; phenolic acids; saponins