Novel turn-on fluorescent detection of alkaline phosphatase based on green synthesized carbon dots and MnO2nanosheets.
Using sterculia lychnophora seeds as precursors for the first time, fluorescent carbon dots (CDs) were synthesized by simple hydrothermal treatment. The quantum yield of as-synthesized CDs was 6.9% by using quinine sulfate as the reference. The fluorescence of CDs could be effectively quenched by a MnO2nanosheet based on fluorescence resonance energy transfer (FRET). Ascorbic acid (AA) could reduce MnO2to Mn2+and result in the destruction of the MnO2nanosheets, which could induce the fluorescence recovery of the CDs. In particular, alkaline phosphatase (ALP) could bio-catalyze acid 2-phosphate (AAP) hydrolysis to AA. Here, an efficient fluorescence probe based on a CDs-MnO2nanosheet for rapid and selective detection of ALP was reported for the first time. Excellent performance for the detection of ALP was observed with high sensitivity and a detection limit of 0.4U/L owing to the low background. The detection of ALP in human serum was conducted with satisfactory results, demonstrating its potential applications in clinical diagnosis.
College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong 273165, PRChina.
Fengli
F
Qu
College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong 273165, PRChina.
Haimeng
H
Pei
College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong 273165, PRChina.
Rongmei
R
Kong
College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong 273165, PRChina.
Shuyun
S
Zhu
College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong 273165, PRChina. Electronic address: xialian01@163.com.
Lian
L
Xia
D000469:Alkaline Phosphatase / Q000097:blood*; D002244:Carbon / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D017895:Manganese Compounds / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry; D010087:Oxides / Q000737:chemistry*; D045663:Quantum Dots
D005456:Fluorescent Dyes; D017895:Manganese Compounds; D010087:Oxides; C027424:manganese oxide; D002244:Carbon; D000469:Alkaline Phosphatase
Alkaline phosphatase; Carbon dots; Fluorescence; MnO(2) nanosheet