Detecting the shape of anisotropic gold nanoparticles in dispersion with single particle extinction and scattering.
The shape and size of nanoparticles are important parameters affecting their biodistribution, bioactivity, and toxicity. The high-throughput characterisation of the nanoparticle shape in dispersion is a fundamental prerequisite for realistic in vitro and in vivo evaluation, however, with routinely available bench-top optical characterisation techniques, it remains a challenging task. Herein, we demonstrate the efficacy of a single particle extinction and scattering (SPES) technique for the in situ detection of the shape of nanoparticles in dispersion, applied to a small library of anisotropic gold particles, with a potential development for in-line detection. The use of SPES paves the way to the routine quantitative analysis of nanoparticles dispersed in biologically relevant fluids, which is of importance for the nanosafety assessment and any in vitro and in vivo administration of nanomaterials.
CIMAINA and Dipartimento di Fisica, Università degli Studi di Milano, Via Celoria, 16, 20133 Milano, Italy. Paolo.Milani@mi.infn.it.
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Potenza
Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Dublin, Ireland. Kenneth.A.Dawson@cbni.ucd.ie and School of Environment and Life Sciences, University of Salford, M5 4WT Salford, UK.
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http://orcid.org/0000-0003-1129-6831
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Krpetić
EOS srl, Viale Ortles 22/4, 20139 Milano, Italy.
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Sanvito
Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Dublin, Ireland. Kenneth.A.Dawson@cbni.ucd.ie.
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Cai
Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Dublin, Ireland. Kenneth.A.Dawson@cbni.ucd.ie.
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Monopoli
Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Dublin, Ireland. Kenneth.A.Dawson@cbni.ucd.ie and Departamento de Física Teórica e Experimental, Universidade Federal do Rio Grande do Norte, 59078-970, Natal, RN, Brazil.
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de Araújo
EOS srl, Viale Ortles 22/4, 20139 Milano, Italy.
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Cella
Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Dublin, Ireland. Kenneth.A.Dawson@cbni.ucd.ie.
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http://orcid.org/0000-0002-2732-5484
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Boselli
Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Dublin, Ireland. Kenneth.A.Dawson@cbni.ucd.ie.
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Castagnola
CIMAINA and Dipartimento di Fisica, Università degli Studi di Milano, Via Celoria, 16, 20133 Milano, Italy. Paolo.Milani@mi.infn.it.
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Milani
Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Dublin, Ireland. Kenneth.A.Dawson@cbni.ucd.ie.
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Dawson