Things Are Getting Hairy: Enterobacteria Bacteriophage vB_PcaM_CBB.
Enterobacteria phage vB_PcaM_CBB is a "jumbo" phage belonging to the familyMyoviridae. It possesses highly atypical whisker-like structures along the length of its contractile tail. It has a broad host range with the capability of infecting species of the generaErwinia, Pectobacterium, andCronobacter. With a genome of 355,922 bp, excluding a predicted terminal repeat of 22,456 bp, phage CBB is the third largest phage sequenced to date. Its genome was predicted to encode 554 ORFs with 33 tRNAs. Based on prediction and proteome analysis of the virions, 29% of its predicted ORFs could be functionally assigned. Protein comparison shows that CBB shares between 33-38% of its proteins with Cronobacter phage GAP32, coliphages PBECO4 and 121Q as well asKlebsiellaphage vB_KleM_Rak2. This work presents a detailed and comparative analysis of vB_PcaM_CBB of a highly atypical jumbo myoviridae phage, contributing to a better understanding of phage diversity and biology.
Department of Biological Sciences, Cork Institute of Technology Cork, Ireland.
Colin
C
Buttimer
Laboratory of Gene Technology, KU Leuven Leuven, Belgium.
Hanne
H
Hendrix
Laboratório de Investigação em Biofilmes Rosário Oliveira, Centre of Biological Engineering, University of Minho Braga, Portugal.
Hugo
H
Oliveira
Teagasc Food Research Centre, Moorepark Fermoy, Co. Cork, Ireland.
Aidan
A
Casey
Department of Microbiology and Biotechnology, Max Rubner-Institut Kiel, Germany.
Horst
H
Neve
Teagasc Food Research Centre, Moorepark Fermoy, Co. Cork, Ireland.
Olivia
O
McAuliffe
Teagasc Food Research Centre, Moorepark Fermoy, Co. Cork, Ireland.
R Paul
RP
Ross
APC Microbiome Institute and School of Microbiology, University College Cork, Ireland.
Colin
C
Hill
Biomedical Research Institute and Transnational University Limburg, Hasselt University Diepenbeek, Belgium.
Jean-Paul
JP
Noben
Department of Biological Sciences, Cork Institute of Technology Cork, Ireland.
Jim
J
O'Mahony
Laboratory of Gene Technology, KU Leuven Leuven, Belgium.
Rob
R
Lavigne
Department of Biological Sciences, Cork Institute of Technology Cork, Ireland.
Aidan
A
Coffey
Jumbo bacteriophage; PFGE analysis; bacteriophages; bioinformatics; genome; host range; mass spectrometry; transmission electron microscopy