Parvalbumin-Neurons of the Ventrolateral Hypothalamic Parvafox Nucleus Receive a Glycinergic Input: A Gene-Microarray Study.
The ventrolateral hypothalamic parvafox (formerly called PV1-Foxb1) nucleus is an anatomical entity of recent discovery and unknown function. With a view to gaining an insight into its putative functional role(s), we conducted a gene-microarray analysis and, armed with the forthcoming data, controlled the results with the Allen databases and the murine BrainStars (B*) database. The parvafox nucleus was specifically sampled by laser-capture microdissection and the transcriptome was subjected to a microarray analysis on Affymetrix chips. Eighty-two relevant genes were found to be potentially more expressed in this brain region than in either the cerebral cortex or the hippocampus. When the expression patterns of these genes were counterchecked in the Allen-Database ofin-situhybridizations and in the B*-microarray database, their localization in the parvafox region was confirmed for thirteen. For nine novel genes, which are particularly interesting because of their possible involvement in neuromodulation, the expression was verified by quantitative real time-PCR. Of particular functional importance may be the occurrence of glycine receptors, the presence of which indicates that the activity of the parvafox nucleus is under ascending inhibitory control.
Anatomy and Program in Neuroscience, Department of Medicine, University of Fribourg Fribourg, Switzerland.
Viktoria
V
Szabolcsi
Anatomy and Program in Neuroscience, Department of Medicine, University of Fribourg Fribourg, Switzerland.
Gioele W
GW
Albisetti
Anatomy and Program in Neuroscience, Department of Medicine, University of Fribourg Fribourg, Switzerland.
Marco R
MR
Celio
Allen Brain Atlas Database of in-situ hybridizations (ABA-ISH); BrainStars (B*) microarrays; GlyT2-EGFP; PV1-Foxb1; glycine receptor α2; laser capture microdissection; parvafox; parvalbumin