Comparison of biofilm formation and motility processes in arsenic-resistant Thiomonas spp. strains revealed divergent response to arsenite.
Bacteria of the genus Thiomonas are found ubiquitously in arsenic contaminated waters such as acid mine drainage (AMD), where they contribute to the precipitation and the natural bioremediation of arsenic. In these environments, these bacteria have developed a large range of resistance strategies among which the capacity to form particular biofilm structures. The biofilm formation is one of the most ubiquitous adaptive response observed in prokaryotes to various stresses, such as those induced in the presence of toxic compounds. This study focused on the process of biofilm formation in three Thiomonas strains (CB1, CB2 and CB3) isolated from the same AMD. The results obtained here show that these bacteria are all capable of forming biofilms, but the architecture and the kinetics of formation of these biofilms differ depending on whether arsenite is present in the environment and from one strain to another. Indeed, two strains favoured biofilm formation, whereas one favoured motility in the presence of arsenite. To identify the underlying mechanisms, the patterns of expression of some genes possibly involved in the process of biofilm formation were investigated in Thiomonas sp. CB2 in the presence and absence of arsenite, using a transcriptomic approach (RNA-seq). The findings obtained here shed interesting light on how the formation of biofilms, and the motility processes contribute to the adaptation of Thiomonas strains to extreme environments.
Laboratoire Génétique moléculaire, Génomique et Microbiologie, UMR7156, CNRS and Université de Strasbourg, Institut de Botanique, Strasbourg, France.
Julien
J
Farasin
Laboratoire Génétique moléculaire, Génomique et Microbiologie, UMR7156, CNRS and Université de Strasbourg, Institut de Botanique, Strasbourg, France.
Sandrine
S
Koechler
Institut Pasteur, Plate-forme Transcriptome et Epigenome, BioMics, Centre d'innovation et recherche technologique, Paris, France.|Institut Pasteur, Hub Bioinformatique et Biostatistique, Centre de Bioinformatique, Biostatistique et Biologie Intégrative (C3BI, USR 3756, IP CNRS), Paris, France.
Hugo
H
Varet
Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.
Julien
J
Deschamps
Institut Pasteur, Plate-forme Transcriptome et Epigenome, BioMics, Centre d'innovation et recherche technologique, Paris, France.|Institut Pasteur, Hub Bioinformatique et Biostatistique, Centre de Bioinformatique, Biostatistique et Biologie Intégrative (C3BI, USR 3756, IP CNRS), Paris, France.
Marie-Agnès
MA
Dillies
Institut Pasteur, Plate-forme Transcriptome et Epigenome, BioMics, Centre d'innovation et recherche technologique, Paris, France.
Caroline
C
Proux
Université de Strasbourg, CNRS, IBMP UPR 2357, F-67000 Strasbourg, France.
Mathieu
M
Erhardt
Laboratoire Génétique moléculaire, Génomique et Microbiologie, UMR7156, CNRS and Université de Strasbourg, Institut de Botanique, Strasbourg, France.
Aline
A
Huber
Institut Pasteur, Plate-forme Transcriptome et Epigenome, BioMics, Centre d'innovation et recherche technologique, Paris, France.
Bernd
B
Jagla
Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.
Romain
R
Briandet
Institut Pasteur, Plate-forme Transcriptome et Epigenome, BioMics, Centre d'innovation et recherche technologique, Paris, France.
Jean-Yves
JY
Coppée
Laboratoire Génétique moléculaire, Génomique et Microbiologie, UMR7156, CNRS and Université de Strasbourg, Institut de Botanique, Strasbourg, France.
Florence
F
Arsène-Ploetze
D018053:Arsenites / Q000378:metabolism*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D000073556:Burkholderiales / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D024881:Drug Resistance, Bacterial; D004785:Environmental Pollutants / Q000378:metabolism*; D020869:Gene Expression Profiling; D008124:Locomotion / Q000187:drug effects*
D018053:Arsenites; D004785:Environmental Pollutants; C015001:arsenite