Production of Potent Antimicrobial Compounds fromStreptomyces cyaneofuscatusAssociated with Fresh Water Sediment.
The genusStreptomycesunder phylum actinobacteria has been recognized as a prolific source for the production of bioactive secondary metabolites. An actinobacterial strain designated as DST103 isolated from a wetland fresh water sediment of Tamdil Lake, Mizoram, Northeast, India was identified asStreptomyces cyaneofuscatus(KY287599) using 16SrRNA gene sequencing which shares 99.87% sequence similarity withStreptomyces cyaneofuscatusNRRL B-2570T. The strain showed broad spectrum antimicrobial activities against Gram negative bacteria (Escherichia coliMTCC 739 andPseudomonas aeruginosaMTCC 2453), Gram positive bacteria (Micrococcus luteusNCIM 2170 andStaphylococcus aureusMTCC 96) and yeast pathogenCandida albicansMTCC 3017). The methanolic extract of the strain DST103 exhibited highest antimicrobial activity againstE. coli(IC50= 2.10 μg/mL) and minimum activity againstS. aureus(IC50= 43.63 μg/mL). Five antibiotics [trimethoprim (18 μg/g), fluconazole (6 μg/g), ketoconazole (18 μg/g), nalidixic acid (135 μg/g), and rifampicin (56 μg/g)] were detected and quantified using ultra-performance liquid chromatography (UPLC-ESI-MS/MS). Further, biosynthetic potential genes [polyketide synthases type II, non-ribosomal peptide synthetases, and aminodeoxyisochorismate synthase (phzE)] were also detected in strain DST103 which may possibly be responsible for the production of antimicrobial compounds. Additionally, gas chromatography-mass spectrometry analysis showed the presence of four volatile compounds which might be responsible for their diverse biological activity. The present study revealed the presence of bioactive compounds in strain DST103, which may be a promising resource for the discovery of novel bioactive metabolites against wide range of pathogens.
Molecular Microbiology and Systematics Laboratory, Department of Biotechnology, Mizoram University Aizawl, India.
Zothanpuia
Molecular Microbiology and Systematics Laboratory, Department of Biotechnology, Mizoram University Aizawl, India.
Ajit K
AK
Passari
CSIR-Central Drug Research Institute, Sophisticated Analytical Instrument Facility Lucknow, India.
Preeti
P
Chandra
Molecular Microbiology and Systematics Laboratory, Department of Biotechnology, Mizoram University Aizawl, India.
Vincent V
VV
Leo
Molecular Microbiology and Systematics Laboratory, Department of Biotechnology, Mizoram University Aizawl, India.
Vineet K
VK
Mishra
CSIR-Central Drug Research Institute, Sophisticated Analytical Instrument Facility Lucknow, India.
Brijesh
B
Kumar
Molecular Microbiology and Systematics Laboratory, Department of Biotechnology, Mizoram University Aizawl, India.
Bhim P
BP
Singh
GC-MS; Streptomyces cyaneofuscatus; UPLC-ESI-MS/MS; antimicrobial activity; biosynthetic genes