Temporo-spacial microanatomical distribution of the murine sodium-dependent ascorbic acid transporters Slc23a1 and Slc23a2 in the kidney throughout development.
The two membrane transporters Slc23a1 and Slc23a2 mediate ascorbic acid uptake into cells. We recently determined the key role of Slc23a1 in renal re-absorption of ascorbic acid in a knockout mouse model. However, the renal spatial and temporal expression patterns of murine Slc23a1 and Slc23a2 are not defined. This study utilizes database evidence combined with experimental confirmation via in-situ hybridization to define the spatial and temporal expression of Slc23a1 in the murine kidney. Slc23a1 is expressed in the early proximal tubule, but not in its precursors during embryonic development, and exclusive proximal tubular expression persists throughout the animal's lifetime. In contrast, Slc23a2 is uniformly expressed in metabolic cell types such as stromal cells. The expression patterns appear to be conserved from rodent lineages to humans.
a University of Manitoba, Department of Human Nutritional Sciences, W569 Duff Roblin Building, 190 Dysart Road, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.|b Molecular and Clinical Nutrition Section, Building 10, Room 4D52 MSC 1372, National Institutes of Health, Bethesda, MD 20892-1372, USA.
Peter K
PK
Eck
b Molecular and Clinical Nutrition Section, Building 10, Room 4D52 MSC 1372, National Institutes of Health, Bethesda, MD 20892-1372, USA.|c Department of Nutrition and Dietetics, Diabetes & Nutritional Sciences Division, School of Medicine, King's College London, Room 3.114 Franklin-Wilkins Building, 150 Stamford Street, London SE1 9NH, UK.
Christopher
C
Corpe
b Molecular and Clinical Nutrition Section, Building 10, Room 4D52 MSC 1372, National Institutes of Health, Bethesda, MD 20892-1372, USA.
Mark A
MA
Levine
D000818:Animals; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D007668:Kidney / Q000254:growth & development / Q000378:metabolism*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D060347:Sodium-Coupled Vitamin C Transporters / Q000235:genetics*; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism*
D012333:RNA, Messenger; D060347:Sodium-Coupled Vitamin C Transporters
Slc23a1; Slc23a2; development; développement; kidney; murine; rein; souris