Anti-Inflammatory and Antioxidative Stress Effects of Oryzanol in Glaucomatous Rabbits.
Purpose.γ-Oryzanol works by anti-inflammatory and radical scavenging activity as a neuroprotective, anticancer, antiulcer, and immunosuppressive agent. The present study was conducted to investigate effect of oryzanol in acute and chronic experimental glaucoma in rabbits.Methods. Effect of oryzanol was evaluated in 5% dextrose induced acute model of ocular hypertension in rabbit eye. Chronic model of glaucoma was induced with subconjunctival injection of 5% of 0.3 ml phenol. Treatment with oryzanol was given for next two weeks after induction of glaucoma. From anterior chamber of rabbit eye aqueous humor was collected to assess various oxidative stress parameters like malondialdehyde, superoxide dismutase, glutathione peroxidase, catalase, nitric oxide, and inflammatory parameters like TNF-αand IL-6. Structural damage in eye was examined by histopathological studies.Results. In acute model of ocular hypertension oryzanol did not alter raised intraocular pressure. In chronic model of glaucoma oryzanol exhibited significant reduction in oxidative stress followed by reduction in intraocular pressure. Oryzanol treatment reduced level of TNF-αand IL-6. Histopathological studies revealed decreased structural damage of trabecular meshwork, lamina cribrosa, and retina with oryzanol treatment.Conclusions. Oryzanol showed protective effect against glaucoma by its antioxidative stress and anti-inflammatory property. Treatment with oryzanol can reduce optic nerve damage.
Institute of Pharmacy, Nirma University, Sarkhej-Gandhinagar Highway, Ahmedabad 382481, India.
Shital S
0000-0003-4211-8202
SS
Panchal
Institute of Pharmacy, Nirma University, Sarkhej-Gandhinagar Highway, Ahmedabad 382481, India.
Rajesh K
RK
Patidar
Institute of Pharmacy, Nirma University, Sarkhej-Gandhinagar Highway, Ahmedabad 382481, India.
Abhishek B
AB
Jha
Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia; Department of Zoology, Faculty of Science, Beni-Suef University, Beni-Suef 62511, Egypt; Nahda University in Beni Suef, Beni-Suef 62511, Egypt.
Ahmed A
0000-0003-0702-8025
AA
Allam
Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Jamaan
J
Ajarem
Institute of Pharmacy, Nirma University, Sarkhej-Gandhinagar Highway, Ahmedabad 382481, India.
Shital B
SB
Butani