Silk-based anisotropical 3D biotextiles for bone regeneration.
Bone loss in the craniofacial complex can been treated using several conventional therapeutic strategies that face many obstacles and limitations. In this work, novel three-dimensional (3D) biotextile architectures were developed as a possible strategy for flat bone regeneration applications. As a fully automated processing route, this strategy as potential to be easily industrialized. Silk fibroin (SF) yarns were processed into weft-knitted fabrics spaced by a monofilament of polyethylene terephthalate (PET). A comparative study with a similar 3D structure made entirely of PET was established. Highly porous scaffolds with homogeneous pore distribution were observed using micro-computed tomography analysis. The wet state dynamic mechanical analysis revealed a storage modulus In the frequency range tested, the storage modulus values obtained for SF-PET scaffolds were higher than for the PET scaffolds. Human adipose-derived stem cells (hASCs) cultured on the SF-PET spacer structures showed the typical pattern for ALP activity under osteogenic culture conditions. Osteogenic differentiation of hASCs on SF-PET and PET constructs was also observed by extracellular matrix mineralization and expression of osteogenic-related markers (osteocalcin, osteopontin and collagen type I) after 28 days of osteogenic culture, in comparison to the control basal medium. The quantification of convergent macroscopic blood vessels toward the scaffolds by a chick chorioallantoic membrane assay, showed higher angiogenic response induced by the SF-PET textile scaffolds than PET structures and gelatin sponge controls. Subcutaneous implantation in CD-1 mice revealed tissue ingrowth's accompanied by blood vessels infiltration in both spacer constructs. The structural adaptability of textile structures combined to the structural similarities of the 3D knitted spacer fabrics to craniofacial bone tissue and achieved biological performance, make these scaffolds a possible solution for tissue engineering approaches in this area.
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, Universidade do Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark - Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco/Guimarães, Portugal; ICVS/3B's - PT Government Associated Laboratory, Braga/Guimarães, Portugal.
Viviana P
VP
Ribeiro
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, Universidade do Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark - Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco/Guimarães, Portugal; ICVS/3B's - PT Government Associated Laboratory, Braga/Guimarães, Portugal.
Joana
J
Silva-Correia
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, Universidade do Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark - Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco/Guimarães, Portugal; ICVS/3B's - PT Government Associated Laboratory, Braga/Guimarães, Portugal.
Ana I
AI
Nascimento
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, Universidade do Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark - Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco/Guimarães, Portugal; ICVS/3B's - PT Government Associated Laboratory, Braga/Guimarães, Portugal.
Alain
A
da Silva Morais
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, Universidade do Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark - Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco/Guimarães, Portugal; ICVS/3B's - PT Government Associated Laboratory, Braga/Guimarães, Portugal.
Alexandra P
AP
Marques
CeNTI - Centre for Nanotechnology and Smart Materials, Rua Fernando Mesquita, 4760-034 Vila Nova de Famalicão, Portugal.
Ana S
AS
Ribeiro
CeNTI - Centre for Nanotechnology and Smart Materials, Rua Fernando Mesquita, 4760-034 Vila Nova de Famalicão, Portugal.
Carla J
CJ
Silva
CITEVE - Technological Center for Textile and Clothing Industry, Rua Fernando Mesquita, 4760-034 Vila Nova de Famalicão, Portugal.
Graça
G
Bonifácio
Stemmatters, Biotecnologia e Medicina Regenerativa SA, AvePark - Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Portugal.
Rui A
RA
Sousa
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, Universidade do Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark - Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco/Guimarães, Portugal; ICVS/3B's - PT Government Associated Laboratory, Braga/Guimarães, Portugal.
Joaquim M
JM
Oliveira
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, Universidade do Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark - Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco/Guimarães, Portugal; ICVS/3B's - PT Government Associated Laboratory, Braga/Guimarães, Portugal; CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Arquiteto Lobão Vital, 4202-401 Porto, Portugal. Electronic address: aloliveira@porto.ucp.pt.
Ana L
AL
Oliveira
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, Universidade do Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark - Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco/Guimarães, Portugal; ICVS/3B's - PT Government Associated Laboratory, Braga/Guimarães, Portugal.
Rui L
RL
Reis
D016880:Anisotropy; D001861:Bone Regeneration / Q000502:physiology*; D018786:Bone Substitutes / Q000138:chemical synthesis*; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*; D066330:Printing, Three-Dimensional; D047011:Silk / Q000737:chemistry*; D013784:Textiles; D054457:Tissue Scaffolds
D018786:Bone Substitutes; D047011:Silk
Biotextile; Craniofacial bone tissue engineering; Human adipose-derived stem cells; Knitted spacer fabrics; Silk fibroin; Textile