Identification of the crucial genes in the elimination and survival process of Salmonella enterica ser. Pullorum in the chicken spleen.
Salmonella enterica ser. Pullorum is one of the most easily re-infecting pathogens in poultry production because of its mechanism of escaping from immune elimination. We used the transcriptome method to investigate the variation in gene expression in chicken spleen resulting from the interaction between hosts and S. Pullorum in the survival process. The expression of various genes related to the maturation and activation of B cells was activated before S. Pullorum was eliminated, which might help S. Pullorum escape from the elimination process. The suppression of some genes involved in the fusion of autophagosomes and lysosomes, such as MYO6, was identified and may be regulated by the secretion systems of S. Pullorum. In addition, a large proportion of these differentially expressed genes could be localized in the identified quantitative trait loci regions associated with the antibody response to bacteria. Collectively, these identified genes provided an outline for further understanding the interaction between chicken immune cells and S. Pullorum in chicken spleen.
Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
T
T
Ma
Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
L
L
Xu
Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
H
H
Wang
Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
X
X
Guo
Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
Z
Z
Li
Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
F
F
Wan
Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
J
J
Chen
Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
L
L
Liu
Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, Jiangsu, 225125, China.
X
X
Liu
Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
G
G
Chang
Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
G
G
Chen
D000818:Animals; D000917:Antibody Formation; D002645:Chickens / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D011201:Poultry Diseases / Q000235:genetics* / Q000276:immunology; D040641:Quantitative Trait Loci; D012481:Salmonella Infections, Animal / Q000235:genetics* / Q000276:immunology; D019779:Salmonella enterica; D013154:Spleen / Q000276:immunology*; D059467:Transcriptome
Salmonella Pullorum; cellular immunity; differentially expressed genes; microarray; survival mechanism