Identification of QTLs for yield-related traits in RILs derived from the cross between pLIA-1 carryingOryza longistaminatachromosome segments and Norin 18 in rice.
To improve rice yield, a wide genetic pool is necessary. It is therefore important to explore wild rice relatives.Oryza longistaminatais a distantly related wild rice relative that carries the AA genome. Its potential for improving agronomic traits is not well studied. Introgression line (pLIA-1) that carriesOryza longistaminata's chromosome segments, showed high performance in yield-related traits under non-fertilized conditions. Therefore, to illustrateOryza longistaminata's potential for improving yield-related traits, RILs from the F1of a cross between pLIA-1 and Norin 18 were developed and QTL analysis was done using the RAD-Seq method. In total, 36 QTLs for yield-related traits were identified on chromosomes 1, 2, 3, 5, 6, 7, 8, 10, and 11. Clusters of QTLs for strongly correlated traits were also identified on chromosomes 1, 3, 6, and 8. Phenotypic data from recombinant plants for chromosomes 1 and 8 QTL clusters revealed that the pLIA-1 genotype on chromosome 1 region was more important for panicle-related traits and a combination of pLIA-1 genotypes on chromosomes 1 and 8 showed a favorable phenotype under non-fertilized conditions. These results suggest thatOryza longistaminata's chromosome segments carry important alleles that can be used to improve yield-related traits of rice.
Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan; Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan.
Emily
E
Gichuhi
Institute of Plant Science and Resources, Okayama University , Kurashiki, Okayama 710-0046 , Japan.
Eiko
E
Himi
Graduate School of Bioresource Sciences, Akita Prefectural University , Akita 010-0195 , Japan.
Hidekazu
H
Takahashi
Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya, Aichi 464-8601 , Japan.
Sinhao
S
Zhu
Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya, Aichi 464-8601 , Japan.
Kazuyuki
K
Doi
National Institute for Basic Biology , Okazaki, Aichi 444-8585 , Japan.
Kazuo
K
Tsugane
Institute of Plant Science and Resources, Okayama University , Kurashiki, Okayama 710-0046 , Japan.
Masahiko
M
Maekawa
Oryza longistaminata; QTLs; RAD-Seq; RIL; chromosome segment