Surface Coating of Gypsum-Based Molds for Maxillofacial Prosthetic Silicone Elastomeric Material: Evaluating Different Microbial Adhesion.
OBJECTIVE
To compare the adhesion of three microorganisms on modified and unmodified silicone elastomer surfaces with different surface roughnesses and porosities.


METHODS
Candida albicans, Streptococcus mutans, and Staphylococcus aureus were incubated with modified and unmodified silicone groups (N = 35) for 30 days at 37°C. The counts of viable microorganisms in the accumulating biofilm layer were determined and converted to cfu/cm2unit surface area. A scanning electron microscope (SEM) was used to evaluate the microbial adhesion. Statistical analysis was performed using t-test, one-way ANOVA, and post hoc tests as indicated.


RESULTS
Significant differences in microbial adhesion were observed between modified and unmodified silicone elastomers after the cells were incubated for 30 days (p < 0.001). SEM showed evident differences in microbial adhesion on modified silicone elastomer compared with unmodified silicone elastomer.


CONCLUSIONS
Surface modification of silicone elastomer yielding a smoother and less porous surface showed lower adhesion of different microorganisms than observed on unmodified surfaces.
Prosthodontics Unit, Faculty of Dentistry, Universiti Sains Malaysia, Kelantan, Malaysia.|Prosthodontics Unit, Faculty of Dentistry, University of Anbar, Anbar, Iraq.
Salah
S
Khalaf
Prosthodontics Unit, Faculty of Dentistry, Universiti Sains Malaysia, Kelantan, Malaysia.
Zaihan
Z
Ariffin
Prosthodontics Unit, Faculty of Dentistry, Universiti Sains Malaysia, Kelantan, Malaysia.
Adam
A
Husein
Prosthodontics Unit, Faculty of Dentistry, Universiti Sains Malaysia, Kelantan, Malaysia.
Fazal
F
Reza
D001422:Bacterial Adhesion; D002133:Calcium Sulfate; D002176:Candida albicans / Q000502:physiology*; D002448:Cell Adhesion; D020099:Coated Materials, Biocompatible; D003764:Dental Materials; D008422:Materials Testing; D008447:Maxillofacial Prosthesis; D011474:Prosthesis Design; D012826:Silicone Elastomers; D013211:Staphylococcus aureus / Q000502:physiology*; D013295:Streptococcus mutans / Q000502:physiology*; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D003764:Dental Materials; D012826:Silicone Elastomers; D002133:Calcium Sulfate
Maxillofacial prostheses; microbial adhesion; silicone elastomers