RAD-Seq analysis of typical and minorCitrusaccessions, including Bhutanese varieties.
We analyzed the reduced-representation genome sequences ofCitrusspecies by double-digest restriction site-associated DNA sequencing (ddRAD-Seq) using 44 accessions, including typical and minor accessions, such as Bhutanese varieties. The results of this analysis using typical accessions were consistent with previous reports that citron, papeda, pummelo, and mandarin are ancestral species, and that mostCitrusspecies are derivatives or hybrids of these four species.Citrusvarieties often reproduce asexually and heterozygosity is highly conserved within each variety. Because this approach could readily detect conservation of heterozygosity, it was able to discriminate citrus varieties such as satsuma mandarin from closely related species. Thus, this method provides an inexpensive way to protect citrus varieties from unintended introduction and to prevent the provision of incorrect nursery stocks to customers. OneCitrusvariety in Bhutan was morphologically similar to Mexican lime and was designated as Himalayan lime. The current analysis confirmed the previous proposition that Mexican lime is a hybrid between papeda and citron, and also suggested that Himalayan lime is a probable hybrid between mandarin and citron. In addition to Himalayan lime, current analysis suggested that several accessions were formed by previously undescribed combinations.
Faculty of Agriculture, Saga University, 1 Honjo-machi, Saga 840-8502, Japan; Renewable Natural Resources Research Centre Wengkhar, Mongar, Bhutan.
Tshering
T
Penjor
Faculty of Agriculture, Saga University , 1 Honjo-machi, Saga 840-8502 , Japan.
Takashi
T
Mimura
Faculty of Agriculture, Saga University , 1 Honjo-machi, Saga 840-8502 , Japan.
Nobuhiro
N
Kotoda
Faculty of Agriculture, Saga University , 1 Honjo-machi, Saga 840-8502 , Japan.
Ryoji
R
Matsumoto
Center for Ecological Research, Kyoto University, 509-3 2-chome, Hirano, Otsu, Shiga 520-2113, Japan; JST PRESTO, 4-1-8, Honcho, Kawaguchi, Saitama 332-0012, Japan; Faculty of Agriculture, Ryukoku University, 1-5 Yokotani, Seta Oe-cho, Otsu, Shiga 520-2194, Japan.
Atsushi J
AJ
Nagano
Center for Ecological Research, Kyoto University , 509-3 2-chome, Hirano, Otsu, Shiga 520-2113 , Japan.
Mie N
MN
Honjo
Center for Ecological Research, Kyoto University , 509-3 2-chome, Hirano, Otsu, Shiga 520-2113 , Japan.
Hiroshi
H
Kudoh
Faculty of Agriculture, Kagoshima University , 1-21-35 Korimoto, Kagoshima 890-0065 , Japan.
Masashi
M
Yamamoto
Analytical Research Center for Experimental Sciences, Saga University , 1 Honjo-machi, Saga 840-8502 , Japan.
Yukio
Y
Nagano
Bhutan; Citrus; RAD-Seq; genetic relationship; high-throughput sequencing; lime