Evaluation of an injectable bioactive borate glass cement to heal bone defects in a rabbit femoral condyle model.
There is a need for synthetic biomaterials to heal bone defects using minimal invasive surgery. In the present study, an injectable cement composed of bioactive borate glass particles and a chitosan bonding solution was developed and evaluated for its capacity to heal bone defects in a rabbit femoral condyle model. The injectability and setting time of the cement in vitro decreased but the compressive strength increased (8±2MPa to 31±2MPa) as the ratio of glass particles to chitosan solution increased (from 1.0gml-1to 2.5gml-1). Upon immersing the cement in phosphate-buffered saline, the glass particles reacted and converted to hydroxyapatite, imparting bioactivity to the cement. Osteoblastic MC3T3-E1 cells showed enhanced proliferation and alkaline phosphatase activity when incubated in media containing the soluble ionic product of the cement. The bioactive glass cement showed a better capacity to stimulate bone formation in rabbit femoral condyle defects at 12weeks postimplantation when compared to a commercial calcium sulfate cement. The injectable bioactive borate glass cement developed in this study could provide a promising biomaterial to heal bone defects by minimal invasive surgery.
Institute of Bioengineering and Information Technology Materials, Tongji University, Shanghai 200092, China; Center for Human Tissues and Organs Degeneration, Shenzhen Institute of Advanced Technology, Chinese Academy of Science, Shenzhen 518055, China.
Xu
X
Cui
Institute of Bioengineering and Information Technology Materials, Tongji University, Shanghai 200092, China.
Wenhai
W
Huang
Department of Spine Surgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China. Electronic address: zhangyadong6@126.com.
Yadong
Y
Zhang
Center for Human Tissues and Organs Degeneration, Shenzhen Institute of Advanced Technology, Chinese Academy of Science, Shenzhen 518055, China.
Chengcheng
C
Huang
Center for Human Tissues and Organs Degeneration, Shenzhen Institute of Advanced Technology, Chinese Academy of Science, Shenzhen 518055, China.
Zunxiong
Z
Yu
Center for Human Tissues and Organs Degeneration, Shenzhen Institute of Advanced Technology, Chinese Academy of Science, Shenzhen 518055, China.
Lei
L
Wang
Center for Human Tissues and Organs Degeneration, Shenzhen Institute of Advanced Technology, Chinese Academy of Science, Shenzhen 518055, China.
Wenlong
W
Liu
Center for Human Tissues and Organs Degeneration, Shenzhen Institute of Advanced Technology, Chinese Academy of Science, Shenzhen 518055, China.
Ting
T
Wang
Institute of Bioengineering and Information Technology Materials, Tongji University, Shanghai 200092, China.
Jie
J
Zhou
Institute of Bioengineering and Information Technology Materials, Tongji University, Shanghai 200092, China.
Hui
H
Wang
Institute of Bioengineering and Information Technology Materials, Tongji University, Shanghai 200092, China.
Nai
N
Zhou
Institute of Bioengineering and Information Technology Materials, Tongji University, Shanghai 200092, China.
Deping
D
Wang
Center for Human Tissues and Organs Degeneration, Shenzhen Institute of Advanced Technology, Chinese Academy of Science, Shenzhen 518055, China. Electronic address: hb.pan@siat.ac.cn.
Haobo
H
Pan
Department of Spine Surgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Department of Materials Science and Engineering, Missouri University of Science and Technology, Rolla, MO 65409-0340, USA. Electronic address: rahaman@mst.edu.
Mohamed N
MN
Rahaman
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001843:Bone Cements / Q000494:pharmacology*; D001881:Borates / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D019245:Compressive Strength; D004195:Disease Models, Animal; D005269:Femur / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology*; D005899:Glass Ionomer Cements / Q000494:pharmacology*; D021621:Imaging, Three-Dimensional; D020007:Implants, Experimental; D007267:Injections; D008422:Materials Testing; D051379:Mice; D061848:Optical Imaging; D011817:Rabbits; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014945:Wound Healing / Q000187:drug effects*; D014961:X-Ray Diffraction; D055114:X-Ray Microtomography
D001672:Biocompatible Materials; D001843:Bone Cements; D001881:Borates; D005899:Glass Ionomer Cements; D000469:Alkaline Phosphatase
Bioactive borate glass; Bone cement; Bone repair; Injectable bioactive glass cement; Rabbit femoral condyle model