A fast and accurate method of detecting Aleutian mink disease virus in blood and tissues of chronically infected mink.
The objective of this study was to assess the sensitivity of the Omni Klentaq-LA DNA polymerase for detecting Aleutian mink disease virus (AMDV) in mink blood and tissues by PCR without DNA extraction. The presence of AMDV DNA was directly tested by Klentaq in the plasma, serum, whole blood, and spleen homogenates of 188 mink 4 and 16 months after inoculation with the virus. Samples from bone marrow, small intestine, liver, lungs, kidneys, and lymph nodes of 20 of the same mink were also tested by Klentaq. DNA was extracted from paired samples of plasma and the aforesaid tissues by a commercial nucleic acid extraction kit (Dynabeads Silane) and tested by PCR. Compared with the extracted DNA, Klentaq detected a significantly greater number of samples in the whole blood, serum, plasma, spleen, and small intestine. It was concluded that Klentaq is a preferred system for directly detecting AMDV DNA in mink blood and tissues. The lower success rate of extracted DNA compared with Klentaq could be the result of DNA losses during the extraction process. This is an important factor in chronically infected mink, which have a low AMDV copy number in the bloodstream. Direct AMDV detection also reduces the cost of PCR amplification and lowers the risk of sample contamination.
Department of Animal Science and Aquaculture, Dalhousie University Faculty of Agriculture, Truro, NS B2N 5E3, Canada.|Department of Animal Science and Aquaculture, Dalhousie University Faculty of Agriculture, Truro, NS B2N 5E3, Canada.
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Farid
Department of Animal Science and Aquaculture, Dalhousie University Faculty of Agriculture, Truro, NS B2N 5E3, Canada.|Department of Animal Science and Aquaculture, Dalhousie University Faculty of Agriculture, Truro, NS B2N 5E3, Canada.
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Rupasinghe
D000453:Aleutian Mink Disease / Q000821:virology; D000454:Aleutian Mink Disease Virus / Q000302:isolation & purification*; D000818:Animals; D002908:Chronic Disease; D004279:DNA, Viral / Q000097:blood; D008907:Mink / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013154:Spleen / Q000821:virology
D004279:DNA, Viral
Aleutian mink disease virus; Dynabeads Silane; Omni Klentaq-LA; détection d’ADN viral; polymerase chain reaction; réaction par polymérase en chaine; viral DNA detection; virus de la maladie aléoutienne du vison