Integration of gold-sputtered electrofluidic paper on wire-included analytical platforms for glucose biosensing.
This work describes the fabrication and evaluation of an electroanalytical paper-based platform based on the combination of both, reusable and disposable materials in order to generate simple, versatile and low-cost microfluidic devices. With this aim, a holder containing metal wires that act as reusable reference and counter electrodes has been developed. The gold-sputtered paper electrode is disposable and easily interchangeable, meanwhile the platform that includes reference and counter electrodes can be reused. The detection zone in the paper is delimited by drawing a hydrophobic line with an inexpensive permanent marker. The effect of experimental variables such as adding solutions through the face where the gold was sputtered (upwards) or through the opposite one (downwards) as well as of other working parameters were studied by cyclic and differential pulse voltammetry with potassium ferrocyanide as a common redox probe and indicator species for enzymatic, immune and DNA biosensing. Enzymatic determination of glucose in real food samples prove the feasibility of the developed system for the construction of electrochemical biosensors.
Departamento de Química Física y Analítica, Facultad de Química, Universidad de Oviedo, 33006 Oviedo, Spain.
Estefanía
E
Núnez-Bajo
Departamento de Química Física y Analítica, Facultad de Química, Universidad de Oviedo, 33006 Oviedo, Spain.
M
M
Carmen Blanco-López
Departamento de Química Física y Analítica, Facultad de Química, Universidad de Oviedo, 33006 Oviedo, Spain.
Agustín
A
Costa-García
Departamento de Química Física y Analítica, Facultad de Química, Universidad de Oviedo, 33006 Oviedo, Spain. Electronic address: mtfernandeza@uniovi.es.
M
M
Teresa Fernández-Abedul
D001628:Beverages / Q000032:analysis*; D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation*; D002253:Carbonated Beverages / Q000032:analysis; D055664:Electrochemical Techniques / Q000191:economics / Q000295:instrumentation*; D004566:Electrodes; D061215:Energy Drinks / Q000032:analysis; D004867:Equipment Design; D005504:Food Analysis / Q000295:instrumentation*; D000067030:Fruit and Vegetable Juices / Q000032:analysis; D005947:Glucose / Q000032:analysis*; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D046210:Microfluidic Analytical Techniques / Q000191:economics / Q000295:instrumentation*; D010209:Paper
D006046:Gold; D005947:Glucose
Electrofluidics; Glucose; Gold-sputtered electrodes; Micro paper electroanalytical devices (µPEDs); Wire-based electrodes