In Vitro and In Vivo Effects of Norathyriol and Mangiferin onα-Glucosidase.
Norathyriol is a metabolite of mangiferin. Mangiferin has been reported to inhibitα-glucosidase. To the best of our knowledge, no study has been conducted to determine or compare those two compounds on inhibitingα-glucosidase in vitro and in vivo by far. In this study, we determined the inhibitory activity of norathyriol and mangiferin onα-glucosidase in vitro and evaluated their antidiabetic effect in diabetic mice. The results showed that norathyriol inhibitedα-glucosidase in a noncompetitive manner with an IC50value of 3.12μM, which is more potent than mangiferin (IC50= 358.54μM) and positive drug acarbose (IC50= 479.2μM) in the zymological experiment. Both of norathyriol and mangiferin caused significant (p< 0.05) reduction in fasting blood glucose and the blood glucose levels at two hours after carbohydrate loading and it was interesting that mangiferin and norathyriol can make the decline of the blood glucose earlier than other groups ever including normal group in the starch tolerance test. However, norathyriol and mangiferin did not significantly influence carbohydrate absorption in the glucose tolerance test. Therefore, the antidiabetic effects of norathyriol and mangiferin might be associated withα-glucosidase, and norathyriol was more potent than mangiferin.
Yunnan University of Traditional Chinese Medicine, Kunming 650500, China.
Zhi-Long
ZL
Shi
Institute of Drug Discovery & Development, Kunming Pharmaceutical Corporation, Kunming 650106, China.
Yi-Dan
YD
Liu
Yunnan University of Traditional Chinese Medicine, Kunming 650500, China.
Yun-Yun
YY
Yuan
Yunnan University of Traditional Chinese Medicine, Kunming 650500, China.
Da
D
Song
Yunnan University of Traditional Chinese Medicine, Kunming 650500, China.
Mei-Feng
MF
Qi
Institute of Drug Discovery & Development, Kunming Pharmaceutical Corporation, Kunming 650106, China.
Xu-Juan
XJ
Yang
Institute of Drug Discovery & Development, Kunming Pharmaceutical Corporation, Kunming 650106, China.
Ping
P
Wang
Institute of Drug Discovery & Development, Kunming Pharmaceutical Corporation, Kunming 650106, China.
Xiao-Ying
XY
Li
Yunnan University of Traditional Chinese Medicine, Kunming 650500, China; Institute of Drug Discovery & Development, Kunming Pharmaceutical Corporation, Kunming 650106, China.
Jian-Hua
0000-0003-0222-086X
JH
Shang
Institute of Drug Discovery & Development, Kunming Pharmaceutical Corporation, Kunming 650106, China.
Zhao-Xiang
0000-0001-6882-2236
ZX
Yang