Serum Alcohol Dehydrogenase and Aldehyde Dehydrogenase Activity in the Course of Hepatitis C.
BACKGROUND
Hepatistis C virus (HCV) affects approximately 170 million people, and it is the leading cause of the chronic liver disease. The destruction of liver cells is reflected by an increase of different enzyme activities in the serum. These enzymes include alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), which play a significant role in the metabolism of many biological substances and exist mainly in the liver. In this study we investigated the activity of alcohol dehydrogenase and its isoenzymes and the total activity of ALDH in the sera of patients with hepatitis C.


METHODS
Serum samples were taken for routine biochemical investigations from 50 patients with hepatitis C and from 50 healthy subjects. The activity of class I and II ADH isoenzymes and ALDH activity were measured by spectrofluorometric methods. For the measurement of total ADH activity and activity of class III and IV isoenzymes, the photometric methods were used.


RESULTS
The analysis of our results shows a statistically significant increase in the activity of ADH I and ADH II (2.5-fold and 2-fold, respectively). Activities of both classes of alcohol dehydrogenase isoenzymes have good correlation with alanine and aspartate aminotransferase. The observed increase in total alcohol dehydrogenase activity was not very high but confirmed the elevation of class I and II isoenzyme activity.


CONCLUSIONS
We can state that the activity of class I and II alcohol dehydrogenase isoenzymes in the sera of patients with hepatitis C is increased and it seems to be caused by the release of these isoenzymes from damaged liver cells.
Wojciech
W
Jelski
Anna
A
Strumnik
Karolina
K
Orywal
Tadeusz W
TW
Lapinski
Magdalena
M
Swiderska
Maciej
M
Szmitkowski
D000328:Adult; D000368:Aged; D000426:Alcohol Dehydrogenase / Q000097:blood*; D000444:Aldehyde Dehydrogenase / Q000097:blood*; D001663:Bilirubin / Q000097:blood; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D006526:Hepatitis C / Q000097:blood* / Q000175:diagnosis / Q000201:enzymology; D006801:Humans; D007527:Isoenzymes; D008099:Liver / Q000201:enzymology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010783:Photometry; D013050:Spectrometry, Fluorescence; D015854:Up-Regulation; D055815:Young Adult
D015415:Biomarkers; D007527:Isoenzymes; C489332:ADH1B protein, human; D000426:Alcohol Dehydrogenase; D000444:Aldehyde Dehydrogenase; D001663:Bilirubin