Down-regulation of C-terminal binding protein 2 (CtBP2) inhibits proliferation, migration, and invasion of human SHSY5Y cells in vitro.
Neuroblastoma is the most common extracranial solid tumor in children and is responsible for ∼15% of pediatric cancer deaths. CtBP2 is a member of the CtBP family of proteins that functions as a transcription regulator and has been demonstrated to interact with the C-terminus of the adenoviral E1A oncoprotein. In this study, the expression of CtBP2 in the human neuroblastoma cell line SHSY5Y was down-regulated using lentiviral-mediated RNA interference. Down-regulation of CtBP2 inhibited the expression of c-myc, MMP2, and MMP9 proteins. Moreover, low expression of CtBP2 resulted in inhibited cell growth, proliferation, migration, and invasion, and the cell cycle was arrested at G2/M-phase. These results indicate that CtBP2 may be a potential target to suppress tumorigenesis in neuroblastoma.
Department of Neurosurgery, The First Hospital of Yancheng, The Fourth Affiliated Hospital of Nantong University, Yancheng 224001, China.
Jiang
J
Nan
Department of Neurosurgery, The First Hospital of Yancheng, The Fourth Affiliated Hospital of Nantong University, Yancheng 224001, China.
Sun
S
Guan
Department of Neurosurgery, The First Hospital of Yancheng, The Fourth Affiliated Hospital of Nantong University, Yancheng 224001, China.
Xu
X
Jin
Department of Neurosurgery, The First Hospital of Yancheng, The Fourth Affiliated Hospital of Nantong University, Yancheng 224001, China.
Zhu
Z
Jian
Department of Neurosurgery, The First Hospital of Yancheng, The Fourth Affiliated Hospital of Nantong University, Yancheng 224001, China.
Fu
F
Linshan
Department of Neurosurgery, The First Hospital of Yancheng, The Fourth Affiliated Hospital of Nantong University, Yancheng 224001, China. Electronic address: guojun8916@163.com.
Guo
G
Jun
D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D056970:Co-Repressor Proteins; D015536:Down-Regulation; D059565:G2 Phase Cell Cycle Checkpoints; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009447:Neuroblastoma; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D034622:RNA Interference
D056970:Co-Repressor Proteins; D009419:Nerve Tissue Proteins; D016271:Proto-Oncogene Proteins c-myc; D000429:Alcohol Oxidoreductases; C421248:CTBP2 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9
CtBP2; Neuroblastoma; Proliferation; SHSY5Y; c-myc