miR-29a modulates SCD expression and is regulated in response to a saturated fatty acid diet in juvenile genetically improved farmed tilapia (Oreochromis niloticus).
MicroRNAs (miRNAs) are small non-coding RNAs that regulate target gene expression by binding to the 3' untranslated region (3' UTR) of the target mRNA. MiRNAs regulate a large variety of genes, including those involved in liver biology and disease. Here, we report for the first time that miR-29a post-transcriptionally regulates stearoyl-CoA desaturase (SCD) by binding to its 3' UTR in genetically improved farmed tilapia (GIFT),Oreochromis niloticus, as shown by a 3' UTR luciferase reporter assay. miR-29a antagomir treatmentin vivoresulted in significant upregulation of SCD expression. We found that miR-29a expression was negatively correlated with SCD expression in GIFT liver. Inhibition of miR-29a led to a significant increase in SCD expression on day 60 induced by a saturated fatty acid diet, thereby increasing conversion of 16:0 and 18:0 to 16:1 and 18:1, respectively, and activating serum insulin, which would favor glucose and lipid uptake by the liver. These results indicate that miR-29a regulates SCD levels by binding to its 3' UTR, and this interaction affects saturated fatty acid stress induction and insulin and lipid accumulation in serum. Our results suggest that miR-29a is critical in regulating lipid metabolism homeostasis in GIFT liver, and this might provide a basis for understanding the biological processes and therapeutic intervention encountered in fatty liver.
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu 214081, China qiangj@ffrc.cn xup@ffrc.cn.
Jun
0000-0003-4718-6618
J
Qiang
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu 214081, China.
Yi Fan
YF
Tao
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu 214081, China.
Jie
J
He
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu 214081, China.
Yi Lan
YL
Sun
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu 214081, China qiangj@ffrc.cn xup@ffrc.cn.
Pao
P
Xu
D020413:3' Untranslated Regions; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D023681:Cichlids / Q000097:blood / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D004032:Diet; D004041:Dietary Fats / Q000378:metabolism; D015536:Down-Regulation; D005227:Fatty Acids / Q000378:metabolism*; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D007328:Insulin / Q000097:blood / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood; D008099:Liver / Q000254:growth & development / Q000502:physiology / Q000648:ultrastructure; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D013230:Stearoyl-CoA Desaturase / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation
D020413:3' Untranslated Regions; D004041:Dietary Fats; D005227:Fatty Acids; D029941:Fish Proteins; D007328:Insulin; D008055:Lipids; D035683:MicroRNAs; D013230:Stearoyl-CoA Desaturase
GIFT; Saturated fatty acid; Stearoyl-CoA desaturase; miR-29a