Biological and mechanical evaluation of a Bio-Hybrid scaffold for autologous valve tissue engineering.
Major challenge in heart valve tissue engineering for paediatric patients is the development of an autologous valve with regenerative capacity. Hybrid tissue engineering approach is recently gaining popularity to design scaffolds with desired biological and mechanical properties that can remodel post implantation. In this study, we fabricated aligned nanofibrous Bio-Hybrid scaffold made of decellularized bovine pericardium: polycaprolactone-chitosan with optimized polymer thickness to yield the desired biological and mechanical properties. CD44+, αSMA+, Vimentin+and CD105-human valve interstitial cells were isolated and seeded on these Bio-Hybrid scaffolds. Subsequent biological evaluation revealed interstitial cell proliferation with dense extra cellular matrix deposition that indicated the viability for growth and proliferation of seeded cells on the scaffolds. Uniaxial mechanical tests along axial direction showed that the Bio-Hybrid scaffolds has at least 20 times the strength of the native valves and its stiffness is nearly 3 times more than that of native valves. Biaxial and uniaxial mechanical studies on valve interstitial cells cultured Bio-Hybrid scaffolds revealed that the response along the axial and circumferential direction was different, similar to native valves. Overall, our findings suggest that Bio-Hybrid scaffold is a promising material for future development of regenerative heart valve constructs in children.
Stem Cell and Molecular Biology Laboratory, Department of Biotechnology, Indian Institute of Technology Madras, Chennai, TN 600036, India; Tissue Culture Laboratory, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojappura, Trivandrum, Kerala 695012, India.
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Jahnavi
Department of Civil Engineering, Indian Institute of Technology Madras, Chennai, TN 600036, India.
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Saravanan
Stem Cell and Molecular Biology Laboratory, Department of Biotechnology, Indian Institute of Technology Madras, Chennai, TN 600036, India.
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Arthi
Department of Engineering Design, Indian Institute of Technology Madras, Chennai, TN 600036, India.
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Bhuvaneshwar
Tissue Culture Laboratory, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojappura, Trivandrum, Kerala 695012, India.
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Kumary
Madras Medical Mission, Institute of Cardio-Vascular Diseases, Mogappair, Chennai, Tamil Nadu 600037, India.
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Rajan
Stem Cell and Molecular Biology Laboratory, Department of Biotechnology, Indian Institute of Technology Madras, Chennai, TN 600036, India. Electronic address: vermars@iitm.ac.in.
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Verma
D000818:Animals; D001696:Biomechanical Phenomena; D002417:Cattle; D002450:Cell Communication; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006350:Heart Valve Prosthesis; D006351:Heart Valves / Q000166:cytology / Q000502:physiology*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010496:Pericardium / Q000378:metabolism; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014182:Transplantation, Autologous
D003094:Collagen
Bio-Hybrid scaffold; Bovine pericardium; Mechanical tests; Polymer; Tissue engineering; Valve interstitial cells