Generation and characterization of mice for conditional inactivation of Zeb1.
The multizinc finger containing transcription factor ZEB1 plays crucial roles during various aspects of mammalian development and tumorigenesis. Best studied in human tumors, ZEB1 is activating the embryo-derived program of epithelial-mesenchymal transition (EMT). The aberrant activation of EMT confers an invasive metastasizing phenotype with acquisition of stem cell properties and resistance to radio- and chemotherapy. Although ZEB1 has very important functions in tumor progression, not much is known about its role in physiological contexts and during development and homeostasis. We describe the generation of Zeb1flox/floxmice carrying a targeted mutation for conditional Zeb1 gene inactivation and show that homozygous Zeb1-depletion in the germline results in a phenotype similar to the conventional Zeb1 knockout.
Department of Experimental Medicine I, Friedrich-Alexander University of Erlangen-Nürnberg, Nikolaus-Fiebiger Center for Molecular Medicine, Erlangen, Germany.
Simone
S
Brabletz
Department of Experimental Medicine I, Friedrich-Alexander University of Erlangen-Nürnberg, Nikolaus-Fiebiger Center for Molecular Medicine, Erlangen, Germany.
María
M
Lasierra Losada
Department of General and Visceral Surgery, Medical Center - University of Freiburg, Faculty of Medicine, Freiburg, Germany.
Otto
O
Schmalhofer
Department of General and Visceral Surgery, Medical Center - University of Freiburg, Faculty of Medicine, Freiburg, Germany.
Julia
J
Mitschke
Department of Experimental Medicine I, Friedrich-Alexander University of Erlangen-Nürnberg, Nikolaus-Fiebiger Center for Molecular Medicine, Erlangen, Germany.
Angela
A
Krebs
Department of Experimental Medicine I, Friedrich-Alexander University of Erlangen-Nürnberg, Nikolaus-Fiebiger Center for Molecular Medicine, Erlangen, Germany.
Thomas
T
Brabletz
Department of Experimental Medicine I, Friedrich-Alexander University of Erlangen-Nürnberg, Nikolaus-Fiebiger Center for Molecular Medicine, Erlangen, Germany.
Marc P
0000-0002-7866-3686
MP
Stemmler
D000818:Animals; D002478:Cells, Cultured; D015536:Down-Regulation; D005347:Fibroblasts / Q000378:metabolism; D055786:Gene Knockout Techniques / Q000379:methods*; D020868:Gene Silencing; D018095:Germ-Line Mutation; D006720:Homozygote; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010641:Phenotype; D000071799:Zinc Finger E-box-Binding Homeobox 1 / Q000235:genetics* / Q000378:metabolism
C519422:ZEB1 protein, mouse; D000071799:Zinc Finger E-box-Binding Homeobox 1
Cre-loxP; cancer; epithelial-mesenchymal transition; metastasis; mouse embryogenesis