Hypomethylation of intron1 of α-synuclein gene does not correlate with Parkinson's disease.
Deregulation of α-synuclein encoding gene (SNCA) is one of the important facets of Parkinson's disease (PD) research. DNA methylation status of SNCA-intron1 has been shown to regulate the α-synuclein expression. The present study is aimed at investigating whether methylation of SNCA-intron1 is associated with higher expression of α-synuclein in PD. We have investigated the intron1 methylation status from 16 post-mortem brain samples comprised of 8 PD and 8 control subjects using bisulfite sequencing. We further correlated this methylation status with α-synuclein protein levels in substantia nigra of that individual using western blot analysis. We did not observe any significant difference in methylation of SNCA-intron1 region between PD and control samples. Moreover, no correlation was observed between methylation of SNCA-intron1 with α-synuclein level. Methylation of SNCA-intron1 region does not correlate with α-synuclein expression in PD samples.
Division of Neurosciences, Burnett School of Biomedical Sciences, University of Central Florida College of Medicine, 6900 Lake Nona Blvd, Orlando, FL, 32827, USA.
Subhrangshu
S
Guhathakurta
Division of Neurosciences, Burnett School of Biomedical Sciences, University of Central Florida College of Medicine, 6900 Lake Nona Blvd, Orlando, FL, 32827, USA.
Baggio A
BA
Evangelista
Division of Neurosciences, Burnett School of Biomedical Sciences, University of Central Florida College of Medicine, 6900 Lake Nona Blvd, Orlando, FL, 32827, USA.
Susmita
S
Ghosh
Division of Neurosciences, Burnett School of Biomedical Sciences, University of Central Florida College of Medicine, 6900 Lake Nona Blvd, Orlando, FL, 32827, USA.
Sambuddha
S
Basu
Division of Neurosciences, Burnett School of Biomedical Sciences, University of Central Florida College of Medicine, 6900 Lake Nona Blvd, Orlando, FL, 32827, USA. Yoon-Seong.kim@ucf.edu.|Kyunghee University Medical College, Seoul, Korea. Yoon-Seong.kim@ucf.edu.
Yoon-Seong
YS
Kim
D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D016022:Case-Control Studies; D019175:DNA Methylation / Q000235:genetics*; D006801:Humans; D007438:Introns / Q000235:genetics*; D008875:Middle Aged; D010300:Parkinson Disease / Q000235:genetics*; D051844:alpha-Synuclein / Q000235:genetics*
D051844:alpha-Synuclein
DNA methylation; Epigenetic regulation; Parkinson’s Disease; α-synuclein