Localization ofBarley yellow dwarf virusMovement Protein Modulating Programmed Cell Death inNicotiana benthamiana.
Barley yellow dwarf virus(BYDV) belongs toLuteovirusand is limited only at phloem related tissues. An open reading frame (ORF) 4 of BYDV codes for the movement protein (MP) of BYDV gating plasmodesmata (PD) to facilitate virus movement. Like other Luteoviruses, ORF 4 of BYDV is embedded in the ORF3 but expressed from the different reading frame in leaky scanning manner. Although MP is a very important protein for systemic infection of BYDV, there was a little information. In this study, MP was characterized in terms of subcellular localization and programmed cell death (PCD). Gene of MP or its mutant (ΔMP) was expressed by Agroinfiltration method. MP was clearly localized at the nucleus and the PD, but ΔMP which was deleted distal N-terminus of MP showed no localization to PD exhibited the different target with original MP. In addition to PD localization, MP appeared associated with small granules in cytoplasm whereas ΔMP did not. MP associated with PD and small granules induced PCD, but ΔMP showed no association with PD and small granules did not exhibit PCD. Based on this study, the distal N-terminal region within MP is seemingly responsible for the localization of PD and the induction small granules and PCD induction. These results suggest that subcellular localization of BYDV MP may modulate the PCD inNicotiana benthamiana.
Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju 54896, Korea.
Jiwon
J
Ju
Division of Biotechnology, Chonbuk National University, Iksan 54596, Korea; Plant Medicinal Research Center, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju 54896, Korea.
Kangmin
K
Kim
Division of Biotechnology, Chonbuk National University, Iksan 54596, Korea; Plant Medicinal Research Center, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju 54896, Korea.
Kui-Jae
KJ
Lee
Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju 54896, Korea; Plant Medicinal Research Center, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju 54896, Korea; Institute of Agricultural Science & Technology, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju 54896, Korea.
Wang Hu
WH
Lee
Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju 54896, Korea; Plant Medicinal Research Center, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju 54896, Korea; Institute of Agricultural Science & Technology, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju 54896, Korea.
Ho-Jong
HJ
Ju
Barley yellow dwarf virus; movement protein; programmed cell death; subcellular localization