Silencing myotubularin related protein 7 enhances proliferation and early differentiation of C2C12 myoblast.
Myotubularin related protein 7 (MTMR7) is a key member of the highly conserved myotubularin related proteins (MTMRs) family, which has phosphatase activity. MTMR7 was increased during myoblast differentiation and exhibited high expression level at primary fibers formation stages in pigs. This suggests that MTMR7 may be involved in myogenesis. In our study, we investigated the roles of MTMR7 on proliferation and differentiation of C2C12 myoblasts. Knocking down MTMR7 not only enhanced myoblast early differentiation via altering the expression of Myf5, but also promoted myoblast proliferation through increasing cyclinA2 expression. The improved proliferation capacity was related to the increased phosphorylation of AKT. Taken together, our research demonstrates that MTMR7 plays an important role in proliferation and early differentiation of C2C12 myoblast.
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, People's Republic of China.
Zhuning
Z
Yuan
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, People's Republic of China.
Yaosheng
Y
Chen
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, People's Republic of China.
Xumeng
X
Zhang
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, People's Republic of China.
Xingyu
X
Zhou
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, People's Republic of China.
Mingsen
M
Li
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, People's Republic of China.
Hu
H
Chen
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, People's Republic of China.
Ming
M
Wu
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, People's Republic of China.
Ying
Y
Zhang
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, People's Republic of China. Electronic address: modelin@mail.sysu.edu.cn.
Delin
D
Mo
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation / Q000502:physiology*; D056751:Cyclin A2 / Q000378:metabolism*; D020868:Gene Silencing / Q000502:physiology; D051379:Mice; D024510:Muscle Development / Q000502:physiology*; D032446:Myoblasts / Q000166:cytology* / Q000502:physiology*; D054558:Protein Tyrosine Phosphatases, Non-Receptor / Q000235:genetics / Q000378:metabolism*
C508184:CCNA2 protein, mouse; D056751:Cyclin A2; C478104:Mtmr7 protein, mouse; D054558:Protein Tyrosine Phosphatases, Non-Receptor
C2C12 myoblast; Knockdown; MTMR7; Myogenesis