Antimicrobial Resistance and Molecular Epidemiology of ESBL-ProducingEscherichia coliIsolated from Outpatients in Town Hospitals of Shandong Province, China.
This study aimed to investigate antimicrobial resistance and molecular epidemiology of extended-spectrum β-lactamase (ESBL)-producingEscherichia coli(E. coli) isolated from outpatients in town hospitals of Shandong province, China. Antimicrobial susceptibility of ESBL-producingE. coliwas tested using the disk diffusion and resistance genes encoding for β-lactamases (blaTEM,blaCTXM, andblaSHV) were detected by polymerase chain reaction (PCR). Multilocus sequence typing (ST) of ESBL-producingE. coliwas analyzed in this study. Our results showed that of 320E. coliisolates, 201 carried ESBL genes (201/320, 62.8%), and these isolates all carriedblaCTX-Mgenes, the most common beingblaCTX-M-14(116/201, 57.7%), followed byblaCTX-M-55(47/201, 23.4%) andblaCTX-M-15(31/201, 15.4%). ESBL-producingE. coliexhibited highly resistant to penicillin derivatives, fluoroquinolones, folate pathway inhibitors, and third-generation cephalosporins, but no carbapenem-resistant isolates were found in this study. Forty-two STs were found among the 201 ESBL-producingE. coli, and the most common ST was ST131 (27/201, 13.4%), followed by ST405 (19/201, 9.5%) and ST69 (15/201, 7.5%). Taken together, a high isolation rate of ESBL-producingE. coli(62.8%) was found among outpatients in town hospitals.blaCTX-Mgene was most dominant and was composed of a variety of subtypes. No dominant ST was detected among ESBL-producingE. coli, indicating that these ESBL-producingE. coliisolates derive from different clones.
Department of Life Sciences, Taishan Medical University Tai'an, China.
Zengmin
Z
Miao
Department of Basic Medicine, Taishan Medical University Tai'an, China.
Song
S
Li
Department of Pediatrics, Maternal and Child Health hospital of Laiwu Laiwu, China.
Lei
L
Wang
Department of Basic Medicine, Taishan Medical University Tai'an, China.
Wengang
W
Song
Disease Controlling Center, Veterinary Bureau of Daiyue Tai'an, China.
Yufa
Y
Zhou
ESBL; ST; antimicrobial resistance; outpatient; town hospital