Changes in the expression of α1B-adrenoceptor in peripheral mononuclear cells correlates with blood pressure and plasmatic homocysteine.
Human peripheral mononuclear cells (HPMC) have been suggested as a practical surrogate for myocardial or vascular cells. Present work analyses if changes in the expression of α1-adrenoceptors (ARs) in HPMC are related to the hypertensive state and its clinical consequences. Quantitative RT-PCR was employed to evaluate the mRNA levels of the three α1-ARs (α1A, α1B, α1D) in HPMC isolated from normotensive and hypertensive patients, and also in tissues from two animal models of hypertension: spontaneously hypertensive rats (SHR) and hypertension induced by chronic treatment with L-NAME. In patients, 24-h ambulatory blood pressure and serum biochemical profile were also recorded. We found that α1B-AR expression was higher in HPMC from hypertensive patients and correlated with blood pressure and plasmatic homocysteine. A rise in the α1B-AR expression in kidneys, but not in heart from hypertensive animal models was also found. α1D-AR did not change in HPMC, not in rat heart or kidney, but a significant correlation with plasmatic aldosterone was found. In conclusion, we have proved that α1-ARs mRNA expression in HPMC correlates with clinical variables and could be used as a potential biomarker in hypertensive patients.
Departamento de Farmacología, Facultad de Farmacia, Universitat de València, Valencia, Spain; Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain. Electronic address: eduardo.oliver@cnic.es.
Eduardo
E
Oliver
Departamento de Farmacología, Facultad de Farmacia, Universitat de València, Valencia, Spain.
Fermí
F
Montó
Hospital de la Ribera, Alzira, Spain.
Eduardo
E
Rovira
Hospital de la Ribera, Alzira, Spain.
Carmen
C
Valldecabres
Hospital de la Ribera, Alzira, Spain.
Vicente
V
Muedra
Departamento de Farmacología, Facultad de Farmacia, Universitat de València, Valencia, Spain.
Pilar
P
D'Ocon
D000328:Adult; D000450:Aldosterone / Q000097:blood; D000818:Animals; D001794:Blood Pressure; D004791:Enzyme Inhibitors; D005260:Female; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D006973:Hypertension / Q000139:chemically induced / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D019331:NG-Nitroarginine Methyl Ester; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D018340:Receptors, Adrenergic, alpha-1 / Q000096:biosynthesis* / Q000235:genetics
D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D018340:Receptors, Adrenergic, alpha-1; D006710:Homocysteine; D000450:Aldosterone; D019331:NG-Nitroarginine Methyl Ester
Alpha1 adrenoceptors; Hypertension; Peripheral mononuclear cells