Protein-surfactant interactions between bovine lactoferrin and sophorolipids under neutral and acidic conditions.
To understand the protein-surfactant interactions between naturally derived sophorolipids (SLs) and bovine lactoferrin (bLf), we carried out spectroscopic, microscopic, and biochemical experiments under weakly acidic and neutral pH conditions. Particle size analysis, microscopy, and enzymatic digestion indicated that bLf and SLs interact with each other to form sheet-like and small aggregated structures reflecting the original self-organization of SLs at pH 5.0 and 7.0, respectively. Circular dichroism (CD) showed that SLs did not significantly affect the secondary structure of bLf.
a Laboratory of Quality Analysis and Assessment, Division of Agronomy and Horticultural Science, Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
Kentaro
K
Matsumiya
b Lactoferrin Laboratory, Saraya Co., Ltd., 2-2-8 Yuzato, Higashisumiyoshi-ku, Osaka 546-0013, Japan.
Yasushi A
YA
Suzuki
b Lactoferrin Laboratory, Saraya Co., Ltd., 2-2-8 Yuzato, Higashisumiyoshi-ku, Osaka 546-0013, Japan.
Yoshihiko
Y
Hirata
a Laboratory of Quality Analysis and Assessment, Division of Agronomy and Horticultural Science, Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
Yuko
Y
Nambu
a Laboratory of Quality Analysis and Assessment, Division of Agronomy and Horticultural Science, Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
Yasuki
Y
Matsumura
D000143:Acids / Q000737:chemistry*; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002417:Cattle; D006017:Glycolipids / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007781:Lactoferrin / Q000494:pharmacology*; D013501:Surface-Active Agents / Q000494:pharmacology*
D000143:Acids; D000890:Anti-Infective Agents; D006017:Glycolipids; D013501:Surface-Active Agents; D007781:Lactoferrin
complex; electrostatic interaction; lactoferrin; lactoferrine, sophorolipides, interaction protéine–surfactant, complexe, interaction électrostatique; protein–surfactant interaction; sophorolipids