Inhibition of Cell Survival by Curcumin Is Associated with Downregulation of Cell Division Cycle 20 (Cdc20) in Pancreatic Cancer Cells.
Pancreatic cancer is one of the most aggressive human tumors in the United States. Curcumin, a polyphenol derived from theCurcuma longaplant, has been reported to exert its antitumor activity in pancreatic cancer. However, the molecular mechanisms of curcumin-mediated tumor suppressive function have not been fully elucidated. In the current study, we explore whether curcumin exhibits its anti-cancer function through inhibition of oncoprotein cell division cycle 20 (Cdc20) in pancreatic cancer cells. We found that curcumin inhibited cell growth, enhanced apoptosis, induced cell cycle arrest and retarded cell invasion in pancreatic cancer cells. Moreover, we observed that curcumin significantly inhibited the expression of Cdc20 in pancreatic cancer cells. Furthermore, our results demonstrated that overexpression of Cdc20 enhanced cell proliferation and invasion, and abrogated the cytotoxic effects induced by curcumin in pancreatic cancer cells. Consistently, downregulation of Cdc20 promoted curcumin-mediated anti-tumor activity. Therefore, our findings indicated that inhibition of Cdc20 by curcumin could be useful for the treatment of pancreatic cancer patients.
Department of Oncology, Guizhou People's Hospital, Guizhou 550002, China. skyline_zyu@163.com.
Yu
Y
Zhang
Department of Oncology, Guizhou People's Hospital, Guizhou 550002, China. xueyingbo0619@163.com.
Ying-Bo
YB
Xue
Department of Oncology, Guizhou People's Hospital, Guizhou 550002, China. lihang.sy@163.com.
Hang
H
Li
Department of Oncology, Guizhou People's Hospital, Guizhou 550002, China. qiudcds@126.com.
Dong
D
Qiu
Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA. zwang6@bidmc.harvard.edu.
Zhi-Wei
ZW
Wang
Department of Oncology, Guizhou People's Hospital, Guizhou 550002, China. tssh18018@126.com.
Shi-Sheng
SS
Tan
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D064199:Cdc20 Proteins / Q000187:drug effects / Q000378:metabolism*; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D003474:Curcumin / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects; D006131:Growth Inhibitors / Q000494:pharmacology; D006801:Humans; D010190:Pancreatic Neoplasms / Q000473:pathology*
D000970:Antineoplastic Agents; D064199:Cdc20 Proteins; D006131:Growth Inhibitors; D003474:Curcumin
Cdc20; curcumin; growth; invasion; pancreatic cancer