Genomic analysis of four strains ofCorynebacterium pseudotuberculosisbv. Equi isolated from horses showing distinct signs of infection.
The genomes of four strains (MB11, MB14, MB30, and MB66) of the speciesCorynebacterium pseudotuberculosisbiovar equi were sequenced on the Ion Torrent PGM platform, completely assembled, and their gene content and structure were analyzed. The strains were isolated from horses with distinct signs of infection, including ulcerative lymphangitis, external abscesses on the chest, or internal abscesses on the liver, kidneys, and lungs. The average size of the genomes was 2.3 Mbp, with 2169 (Strain MB11) to 2235 (Strain MB14) predicted coding sequences (CDSs). An optical map of the MB11 strain generated using the KpnI restriction enzyme showed that the approach used to assemble the genome was satisfactory, producing good alignment between the sequence observed in vitro and that obtainedin silico. In the resulting Neighbor-Joining dendrogram, theC. pseudotuberculosisstrains sequenced in this study were clustered into a single clade supported by a high bootstrap value. The structural analysis showed that the genomes of the MB11 and MB14 strains were very similar, while the MB30 and MB66 strains had several inverted regions. The observed genomic characteristics were similar to those described for other strains of the same species, despite the number of inversions found. These genomes will serve as a basis for determining the relationship between the genotype of the pathogen and the type of infection that it causes.
Federal University of Pará, Institute of Biological Sciences, Center of Genomics and Systems Biology, Belém, Brazil.
Rafael A
RA
Baraúna
Federal University of Pará, Institute of Biological Sciences, Center of Genomics and Systems Biology, Belém, Brazil.
Rommel T J
RTJ
Ramos
Federal University of Pará, Institute of Biological Sciences, Center of Genomics and Systems Biology, Belém, Brazil.
Adonney A O
AAO
Veras
Federal University of Pará, Institute of Biological Sciences, Center of Genomics and Systems Biology, Belém, Brazil.
Pablo H C G
PHCG
de Sá
Federal University of Pará, Institute of Biological Sciences, Center of Genomics and Systems Biology, Belém, Brazil.
Luís C
LC
Guimarães
Federal University of Pará, Institute of Biological Sciences, Center of Genomics and Systems Biology, Belém, Brazil.
Diego A
DA
das Graças
Federal University of Pará, Institute of Biological Sciences, Center of Genomics and Systems Biology, Belém, Brazil.
Adriana R
AR
Carneiro
Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA USA.
Judy M
JM
Edman
Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA USA.
Sharon J
SJ
Spier
Federal University of Minas Gerais, Institute of Biological Sciences, Laboratory of Cellular and Molecular Genetics, Belo Horizonte, Brazil.
Vasco
V
Azevedo
Federal University of Pará, Institute of Biological Sciences, Center of Genomics and Systems Biology, Belém, Brazil.
Artur
A
Silva
Biovar equi; C. pseudotuberculosis; Genomic; Horse; Ulcerative lymphangitis