Calcium concentration dependent collagen mineralization.
Mineralization of collagen fibrils is a regular combination process of organic and mineral components mainly involving calcium, phosphate and collagen. We report the influence of calcium to the self-assembly of collagen by changing the concentration of calcium ion in the process of mineralization. Low concentration of calcium results in the well collagen self-assembly while poor mineral crystallization. Relatively, high concentration of calcium can hinder collagen self-assembly, whereas it is benefited to mineral crystallization. We also reveal that collagen self-assembly happens in advance of the formation of better mineral crystals. These results interpret the mechanism of collagen mineralization further.
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China; Research Institute of Beihang University in Shenzhen, Shenzhen 518057, China. Electronic address: nxf@buaa.edu.cn.
Xufeng
X
Niu
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
Rui
R
Fan
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
Feng
F
Tian
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
Xiaolin
X
Guo
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
Ping
P
Li
State Key Laboratory of New Ceramic and Fine Processing, Tsinghua University, Beijing 100084, China.
Qingling
Q
Feng
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China; National Research Center for Rehabilitation Technical Aids, Beijing 100176, China. Electronic address: yubofan@buaa.edu.cn.
Yubo
Y
Fan
D000818:Animals; D002118:Calcium / Q000032:analysis*; D002152:Calorimetry, Differential Scanning; D003094:Collagen / Q000737:chemistry*; D007477:Ions; D018625:Microscopy, Atomic Force; D008903:Minerals / Q000737:chemistry*; D011489:Protein Denaturation; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014961:X-Ray Diffraction
D007477:Ions; D008903:Minerals; D003094:Collagen; D002118:Calcium
Calcium; Collagen; Mineralization; Self-assembly