Development of a platform for breeding by design of CMS restorer lines based on an SSSL library in rice (Oryza sativaL.).
Exploitation of the heterosis of hybrid rice has shown great success in the improvement of rice yields. However, few genotypes exhibit strong restoration ability as effective restorers of cytoplasmic male sterility (CMS) in the development of hybrid rice. In this study, we developed a platform for the breeding by design of CMS restorer lines based on a library of chromosomal single segment substitution lines (SSSLs) in the Huajingxian74 (HJX74) genetic background. The target genes for breeding by design,Rf34andRf44, which are associated with a strong restoration ability, andgs3,gw8,Wxg1andAlk, which are associated with good grain quality, were selected from the HJX74 SSSL library. Through pyramiding of the target genes, a restorer line, H121R, was developed. The H121R line was then improved regarding blast resistance by pyramiding of theqBLAST11gene. Hence, a new restorer line with blast resistance, H131R, was developed. The platform involving theRf34andRf44restorer genes would be used for the continuous improvement of restorer lines through breeding by design in rice.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University , Guangzhou 510642 , China.
Ziju
Z
Dai
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University , Guangzhou 510642 , China.
Qing
Q
Lu
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University , Guangzhou 510642 , China.
Xin
X
Luan
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University , Guangzhou 510642 , China.
Lian
L
Ouyang
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University , Guangzhou 510642 , China.
Jie
J
Guo
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University , Guangzhou 510642 , China.
Jiayan
J
Liang
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University , Guangzhou 510642 , China.
Haitao
H
Zhu
The Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences , Guangzhou 510640 , China.
Wenjuan
W
Wang
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University , Guangzhou 510642 , China.
Shaokui
S
Wang
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University , Guangzhou 510642 , China.
Ruizhen
R
Zeng
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University , Guangzhou 510642 , China.
Ziqiang
Z
Liu
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University , Guangzhou 510642 , China.
Zemin
Z
Zhang
The Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences , Guangzhou 510640 , China.
Xiaoyuan
X
Zhu
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University , Guangzhou 510642 , China.
Guiquan
G
Zhang
breeding by design; cytoplasmic male sterility; hybrid rice; restorer; single segment substitution line