Plastic antibody based surface plasmon resonance nanosensors for selective atrazine detection.
This study reports a surface plasmon resonance (SPR) based affinity sensor system with the use of molecular imprinted nanoparticles (plastic antibodies) to enhance the pesticide detection. Molecular imprinting based affinity sensor is prepared by the attachment of atrazine (chosen as model pesticide) imprinted nanoparticles onto the gold surface of SPR chip. Recognition element of the affinity sensor is polymerizable form of aspartic acid. The imprinted nanoparticles were characterized via FTIR and zeta-sizer measurements. SPR sensors are characterized with atomic force microscopy (AFM), scanning electron microscopy (SEM), Fourier transform infrared spectrophotometry (FTIR) and contact angle measurements. The imprinted nanoparticles showed more sensitivity to atrazine than the non-imprinted ones. Different concentrations of atrazine solutions are applied to SPR system to determine the adsorption kinetics. Langmuir adsorption model is found as the most suitable model for this affinity nanosensor system. In order to show the selectivity of the atrazine-imprinted nanoparticles, competitive adsorption of atrazine, simazine and amitrole is investigated. The results showed that the imprinted nanosensor has high selectivity and sensitivity for atrazine.
Department of Chemistry, Aksaray University, 68100 Aksaray, Turkey.
Erkut
E
Yılmaz
Department of Chemistry, Hacettepe University, 06800 Ankara, Turkey.
Erdoğan
E
Özgür
Department of Chemistry, Hacettepe University, 06800 Ankara, Turkey.
Nilay
N
Bereli
Department of Chemistry, Hacettepe University, 06800 Ankara, Turkey.
Deniz
D
Türkmen
Department of Chemistry, Hacettepe University, 06800 Ankara, Turkey. Electronic address: denizli@hacettepe.edu.tr.
Adil
A
Denizli
D000640:Amitrole / Q000032:analysis; D000906:Antibodies / Q000378:metabolism*; D001280:Atrazine / Q000032:analysis*; D007700:Kinetics; D054802:Molecular Imprinting; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010969:Plastics / Q000737:chemistry*; D015203:Reproducibility of Results; D012839:Simazine / Q000032:analysis; D012996:Solutions; D020349:Surface Plasmon Resonance / Q000379:methods*; D013696:Temperature
D000906:Antibodies; D010969:Plastics; D012996:Solutions; D001280:Atrazine; D012839:Simazine; D000640:Amitrole
Atrazine; Micro-pollutants; Plastic antibody; SPR MIP nanosensor