Reference genes for gene expression studies in the mouse heart.
To be accurate, quantitative Polymerase Chain Reaction (qPCR) studies require a set of stable reference genes for normalization. This is especially critical in cardiac research because of the diversity of the clinical and experimental conditions in the field. We analyzed the stability of previously described as potential reference genes in different subsets of cardiac tissues, each representing a different field in cardiac research. The qPCR dataset was based on 119 different tissue samples derived from cardiac development to pathology in mouse adult hearts. These samples were grouped into 47 tissue types. The stability of 9 candidate genes was analyzed in each of 12 experimental conditions comprising different groupings of these tissue types. Expression stability was determined with the geNorm module of qbase+. This analysis showed that different sets of two or three reference genes are required for analysis of qPCR data in different experimental conditions in murine cardiac research.
Department of Anatomy, Embryology and Physiology, Academic Medical Center, Amsterdam, The Netherlands.|Cell Therapy, Foundation of Applied Medical Research (FIMA), University of Navarra, Pamplona, Spain.
Adrián
A
Ruiz-Villalba
Department of Anatomy, Embryology and Physiology, Academic Medical Center, Amsterdam, The Netherlands.
Andrea
A
Mattiotti
Department of Anatomy, Embryology and Physiology, Academic Medical Center, Amsterdam, The Netherlands.
Quinn D
QD
Gunst
Department of Animal Biology, University of Málaga, Málaga, Spain.
Sara
S
Cano-Ballesteros
Department of Anatomy, Embryology and Physiology, Academic Medical Center, Amsterdam, The Netherlands.
Maurice J B
MJ
van den Hoff
Department of Anatomy, Embryology and Physiology, Academic Medical Center, Amsterdam, The Netherlands. j.m.ruijter@amc.uva.nl.
Jan M
JM
Ruijter
D000818:Animals; D066264:Datasets as Topic; D020869:Gene Expression Profiling / Q000379:methods* / Q000592:standards*; D006321:Heart / Q000502:physiology*; D017403:In Situ Hybridization; D051379:Mice; D009206:Myocardium / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods / Q000592:standards; D012015:Reference Standards