Detection of cervical intraepithelial neoplasia with HPVE6/E7 mRNA among women with atypical squamous cells of unknown significance.
OBJECTIVE
To compare E6/E7 mRNA and HPV DNA assays for evaluating women with atypical cells of undetermined significance (ASCUS).


METHODS
The present prospective study enrolled patients with ASCUS undergoing HPV testing at Third Affiliated Hospital of Zhengzhou University, China, between September 1, 2013, and January 31, 2016. Patients with positive HPV DNA test results underwent screening by E6/E7 mRNA assay, and the accuracy of HPV DNA and E6/E7 mRNA testing were compared, with histology used for definitive diagnoses.


RESULTS
In total, 591 patients with ASCUS underwent HPV DNA screening, with 455 and 136 having positive and negative results, respectively; 252 patients with positive results and 66 with negative results underwent biopsy and histology testing and were included in the study. The sensitivity of the E6/E7 mRNA assay was similar to that of HPV DNA testing (88.2%, 95% confidence interval [CI] 77.6-94.4 vs 90.7%, 95%CI 81.2-95.9; P=0.636) for the detection of cervical intraepithelial neoplasia grade 2+, and the specificity was higher (36.4%, 95%CI 29.6-43.9 vs 24.3%, 95%CI 19.1-30.3; P=0.006). The area under the receiver operating characteristic curve was greater for E6/E7 mRNA testing compared with HPV DNA testing (0.658 vs 0.588).


CONCLUSIONS
The higher specificity of the E6/E7 mRNA assay means it could be a promising technique in the management of women with ASCUS.
Department of Cytopathology, Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Ya
Y
Li
Department of Cytopathology, Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Shouhua
S
Rong
Department of Cytopathology, Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Yanfang
Y
Zhi
Department of Cytopathology, Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Cui
C
Qiu
Department of Cytopathology, Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Tingting
T
Fan
Department of Cytopathology, Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Xiaofu
X
Li
D000328:Adult; D002681:China; D004279:DNA, Viral / Q000032:analysis*; D018450:Disease Progression; D005260:Female; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000473:pathology / Q000821:virology*; D011446:Prospective Studies; D012367:RNA, Viral / Q000032:analysis*; D012372:ROC Curve; D012680:Sensitivity and Specificity; D016387:Women's Health; D002578:Uterine Cervical Dysplasia / Q000473:pathology / Q000821:virology*
D004279:DNA, Viral; D012367:RNA, Viral

ASCUS
; 
HPV
; CIN2+; E6/E7 mRNA; Management