Effect of dental restorative materials on total antioxidant capacity and calcium concentration of unstimulated saliva.
BACKGROUND
To evaluate the effect of dental amalgam and composite restorations on total antioxidant capacity (TAC) and calcium (Ca) ion concentration of unstimulated saliva.


METHODS
Forty-eight children aged 6-10 years selected and divided into three groups of sixteen (8 males, 8 females). In group A and B, samples consisted of two class II dental composite or amalgam restorations, while in group C samples were caries-free (control group). Unstimulated saliva from all samples was collected and TAC was measured by spectrophotometry using an adaptation of 2, 2'-azino-di-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) assay. The Ca ion level was estimated by an auto- analyzer. Data were analyzed with one- and two-way ANOVA test, at ap<.05 level of significance.


RESULTS
Composite samples showed significantly higher TAC and lower Ca ion levels compared to amalgam and caries-free samples (p<.05). The TAC values showed only significant difference between groups (p<.05), while the Ca ion results showed significant differences within and between groups (p<.05).


CONCLUSIONS
Dental composite restorations increased TAC and decreased Ca ion levels more than amalgam restorations in saliva. Gender is an effective factor in changes induced in oral cavity as females showed more emphatic reaction to dental filling materials than males.
Patients who have dental restorations, especially dental composites, should pay more attention to their dental hygiene, because dental restorations can increase oxidative stress and decrease Ca ion level in saliva, which might jeopardize remineralization process of tooth structures after demineralization.Key words:Amalgam, caries, composite, saliva, total antioxidant capacity.
BDS, MS, DDS, Associate professor, Department of Pediatric Dentistry, Dental Branch, Islamic Azad University, Tehran, Iran.
Gholam H
GH
Ramezani
DDS Visiting researcher, Angiogenesis and Regenerative Group, Dr. H. Afsar Lajevardi Research Cluster, Shiraz, Iran.
Mona-Momeni
MM
Moghadam
BSc, MSc, PhD, Wisconsin institute for Medical Research and Department of Ophthalmology & Visual Sciences, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA.
Mohammad-Ali
MA
Saghiri
DDS, MSc, PhD, Bioscience Research Center, Health Science Center, College of Dentistry, University of Tennessee, Memphis, TN, USA.
Franklin
F
Garcia-Godoy
DDS, Clinical instructor, Angiogenesis and Regenerative Sector, Dr. H. Afsar Lajevardi Research Cluster, Shiraz, Iran.
Armen
A
Asatourian
DDS, MSc, Associate professor, Endodontic Department, School of Dentistry, Tehran University of Medical Sciences and AJA University of Medical Sciences, Tehran, Iran.
Mohsen
M
Aminsobhani
DDS, PhD, Professor, Bioscience Research Center, Health Science Center, College of Dentistry, University of Tennessee, Memphis, TN, USA.
Mark
M
Scarbecz
BSc, MSc, PhD, Professor, Departments of Ophthalmology & Visual Sciences, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA.
Nader
N
Sheibani