Zinc Sulfate and/or Growth Hormone Administration for the Prevention of Radiation-Induced Dermatitis: a Placebo-Controlled Rat Model Study.
Growth hormone (GH) and zinc (Zn) were evaluated for their potential to prevent radiation injury using a rat model of radiation-induced skin injury. Sprague-Dawley rats were divided into five groups: a control group not receiving Zn, GH, or irradiation: a radiation (RT) group receiving a single 30 Gy dose of gamma irradiation to the right hind legs; a radiation + GH group (RT + GH) receiving a single 30 Gy dose of gamma irradiation plus the subcutaneous administration of 0.01 IU kg d-1GH; a radiation + Zn group (RT + Zn) receiving a single 30 Gy dose plus 5 mg kg d-1Zn po; and a radiation + GH + Zn group (RT + GH + Zn) group receiving a single 30 Gy dose plus subcutaneous 0.01 IU kg d-1GH and 5 mg kg d-1Zn po. Acute skin reactions were assessed every 3 days by two radiation oncologists grouping. Light microscopic findings were assessed blindly by two pathologists. Groups receiving irradiation were associated with dermatitis as compared to the control group (P < 0.05). The severity of radiodermatitis in the RT + GH, RT + Zn, and RT + GH + Zn groups was significantly lower than that in the RT group (P < 0.05). Furthermore, radiodermatitis was observed earlier in the RT group than in the other treatment groups (P < 0.05). GH and Zn effectively prevented epidermal atrophy, dermal degeneration, and hair follicle atrophy. The highest level of protection against radiation dermatitis was observed in the combination group.
Department of Radiation Oncology, Faculty of Medicine, Karadeniz Technical University, 61100, Trabzon, Turkey. mkandaz61@gmail.com.
Mustafa
M
Kandaz
Department of Radiation Oncology, Avrasya Hospital, Beştelsiz Mah., 101., Sok., No:107, Akşemsettin Tramvay Durağı, Zeytinburnu, 34020, Istanbul, Turkey.
Mustafa Vecdi
MV
Ertekin
Department of Radiation Oncology, Medikal Park Hospital, Olgunlar Mahallesi, Atatürk Bulvarı, No:5, 23040, Elazığ, Turkey.
İhsan
İ
Karslıoğlu
Department of Pathology, Faculty of Medicine, Atatürk University, 25240, Erzurum, Turkey.
Fazlı
F
Erdoğan
Department of Radiation Oncology, Faculty of Medicine, Atatürk University, 25240, Erzurum, Turkey.
Orhan
O
Sezen
Department of Pharmacology, Faculty of Medicine, Abant İzzet Baysal University, Gölköy Yerleşkesi, 14300, Bolu, Turkey.
Akçahan
A
Gepdiremen
Department of Pathology, Faculty of Medicine, Atatürk University, 25240, Erzurum, Turkey.
Cemal
C
Gündoğdu
D000818:Animals; D004195:Disease Models, Animal; D004311:Double-Blind Method; D005720:Gamma Rays; D013006:Growth Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D011855:Radiodermatitis / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019287:Zinc Sulfate / Q000008:administration & dosage / Q000494:pharmacology*
D019287:Zinc Sulfate; D013006:Growth Hormone
Dermatitis; Growth hormone; Prevention; Radiotherapy; Rat; Zinc sulfate