An Easy Method to Eliminate the Effect of Lupus Anticoagulants in the Coagulation Factor Assay.
BACKGROUND
To build and evaluate intrinsic coagulation factor assays which can eliminate the effect of lupus anticoagulants (LAC).


METHODS
Commercial silica clotting time confirmatory (SCT-C) reagent containing sufficient synthetic phospholipid and routine activated partial thromboplastin time (APTT) reagent were each used for one-stage detection of FVIII, FIX, and FXI activities, in samples with or without LAC, and the results were compared.


RESULTS
For samples without LAC, consistent results of FVIII, FIX, and FXI using both SCT-C reagent and APTT reagent were obtained. For samples with LAC, the assays with SCT-C reagent not only could eliminate the effect of strong lupus anticoagulants but also needed fewer dilutions than that with routine APTT reagent.


CONCLUSIONS
The intrinsic factor detections by SCT-C reagent are credible and convenient to be used for samples with LAC.
Ning
N
Tang
Shiyu
S
Yin
D001780:Blood Coagulation Tests; D002138:Calibration; D005164:Factor IX / Q000032:analysis*; D005169:Factor VIII / Q000032:analysis*; D005172:Factor XI / Q000032:analysis*; D006801:Humans; D007202:Indicators and Reagents; D016682:Lupus Coagulation Inhibitor; D010314:Partial Thromboplastin Time; D014914:Whole Blood Coagulation Time
D007202:Indicators and Reagents; D016682:Lupus Coagulation Inhibitor; C078147:F8 protein, human; D005169:Factor VIII; D005164:Factor IX; D005172:Factor XI