Evaluation of the yield of abiotic-stress-tolerantAtDREB1Atransgenic potato under saline conditions in advance of field trials.
Cultivated potato is a drought-, salinity-, and frost-sensitive species. The transgenic approach is one of the methods used to mitigate abiotic stress. The utility of transgenic potatoes that have abiotic stress tolerance should be judged from their yield under stress conditions. In order to establish transgenic potato lines with theAtDREB1Agene that could be used in practical applications, we screened candidate lines in a growth room with growth profiles under non-stress conditions rather than the expression level of transgene. After identifying better transgenic lines (D163 and D164), yield of those lines under stress conditions was evaluated in the special netted-house. Although the yield was lower than the yield under non-stress conditions, two selected transgenic lines were able to maintain their yield under high saline conditions (EC > 10 mS/cm). In this study, fertilizer was not added beyond what was already contained in the soil mix in order to evaluate the yield of the transgenic lines under saline conditions in as simple a manner as possible. In future studies, it will be necessary to evaluate their yield in a farming context in an isolated field after assessing the environmental biosafety of these transgenic potato lines.
Faculty of Life and Environmental Sciences, Gene Research Center, University of Tsukuba , 1-1-1 Ten-noudai, Tsukuba, Ibaraki 305-8752 , Japan.
Takayoshi
T
Shimazaki
Faculty of Life and Environmental Sciences, Gene Research Center, University of Tsukuba , 1-1-1 Ten-noudai, Tsukuba, Ibaraki 305-8752 , Japan.
Tsukasa
T
Endo
Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS) , Tsukuba, Ibaraki 305-8686 , Japan.
Mie
M
Kasuga
Graduate School of Agricultural and Life Sciences, The University of Tokyo , Bunkyo-ku, Tokyo 113-8657 , Japan.
Kazuko
K
Yamaguchi-Shinozaki
Faculty of Life and Environmental Sciences, Gene Research Center, University of Tsukuba , 1-1-1 Ten-noudai, Tsukuba, Ibaraki 305-8752 , Japan.
Kazuo N
KN
Watanabe
Faculty of Life and Environmental Sciences, Gene Research Center, University of Tsukuba , 1-1-1 Ten-noudai, Tsukuba, Ibaraki 305-8752 , Japan.
Akira
A
Kikuchi
AtDREB1A; abiotic stress tolerance; potato; yield evaluation