Flow cytometry analysis of platelet populations: usefulness for monitoringthe storage lesion in pooled buffy-coat platelet concentrates.
Early detection of the platelet storage lesion is still a challenge in transfusion practice. Using flow cytometry, we evaluated the appearance of the storage lesion, based on the expression of platelet activation markers, in total platelets and platelet populations.



Buffy-coat-derived platelet concentrates were stored under standard conditions for 5 days. The expression of activation antigens CD42b, CD36, CD62p and phosphatidylserine on total platelets and populations of small, medium-sized and large platelets was analysed by flow cytometry on storage days 1, 3 and 5.



The activation/lesion on total platelets and each platelet population was detected on storage day 3, by the increased expression of CD36. On the same day, increased expression of CD42b and CD62p was detected, but only on large platelets. Small and medium-sized platelets had increased CD62p expression only on day 5. Externalisation of phosphatidylserine was not detected.



Evaluation of the level of expression of various activation markers on different platelet populations could be an additional valid analysis in cell quality control of platelet concentrates, and in the assessment of novel approaches to platelet concentrate manipulation.
Institute for Transfusiology and Haemobiology, Military Medical Academy, Belgrade, Serbia.|Faculty of Medicine of Military Medical Academy, University of Defence, Belgrade, Serbia.
Dušan
D
Vučetić
Institute for Medical Research, University of Belgrade, Belgrade, Serbia.
Vesna
V
Ilić
Faculty of Medicine of Military Medical Academy, University of Defence, Belgrade, Serbia.|Institute of Medical Research, Military Medical Academy, Belgrade, Serbia.
Danilo
D
Vojvodić
Faculty of Medicine of Military Medical Academy, University of Defence, Belgrade, Serbia.|Institute of Medical Biochemistry, Military Medical Academy, Belgrade, Serbia.
Vesna
V
Subota
Haematology Clinic of the Clinical Centre of Serbia, Belgrade, Serbia.
Milena
M
Todorović
Institute for Transfusiology and Haemobiology, Military Medical Academy, Belgrade, Serbia.|Faculty of Medicine of Military Medical Academy, University of Defence, Belgrade, Serbia.|Institute for Medical Research, University of Belgrade, Belgrade, Serbia.|Serbian Academy of Sciences and Arts, Belgrade, Serbia.
Bela
B
Balint
D000328:Adult; D015703:Antigens, CD / Q000378:metabolism*; D058621:Blood Buffy Coat / Q000166:cytology / Q000378:metabolism*; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism*; D001793:Blood Preservation; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D013997:Time Factors
D015703:Antigens, CD