On the micro-indentation of plant cells in a tissue context.
The effect of geometry on cell stiffness measured with micro-indentation techniques has been explored in single cells, however it is unclear if results on single cells can be readily transferred to indentation experiments performed on a tissue in vivo. Here we explored this question by using simulation models of osmotic treatments and micro-indentation experiments on 3D multicellular tissues with the finite element method. We found that the cellular context does affect measured cell stiffness, and that several cells of context in each direction are required for optimal results. We applied the model to micro-indentation data obtained with cellular force microscopy on the sepal of A. thaliana, and found that differences in measured stiffness could be explained by cellular geometry, and do not necessarily indicate differences in cell wall material properties or turgor pressure.
Department of Comparative Development and Genetics, Max Planck Institute for Plant Breeding Research, Cologne, Germany. Institute of Plant Sciences, University of Bern, Bern, Switzerland.
Gabriella
G
Mosca
Aleksandra
A
Sapala
Soeren
S
Strauss
Anne-Lise
AL
Routier-Kierzkowska
Richard S
RS
Smith
D017360:Arabidopsis / Q000166:cytology*; D001696:Biomechanical Phenomena; D003198:Computer Simulation; D004548:Elasticity; D020342:Finite Element Analysis; D008954:Models, Biological; D009997:Osmotic Pressure; D059010:Single-Cell Analysis