Rapamycin/sodium hyaluronate binding on nano-hydroxyapatite coated titanium surface improves MC3T3-E1 osteogenesis.
Endosseous titanium (Ti) implant failure due to poor biocompatibility of implant surface remains a major problem for osseointegration. Improving the topography of Ti surface may enhance osseointegration, however, the mechanism remains unknown. To investigate the effect of modified Ti surface on osteogenesis, we loaded rapamycin (RA) onto nano-hydroxyapatite (HAp) coated Ti surface which was acid-etched, alkali-heated and HAp coated sequentially. Sodium hyaluronate (SH) was employed as an intermediate layer for the load of RA, and a steady release rate of RA was maintained. Cell vitality of MC3T3-E1 was assessed by MTT. Osteogenesis of MC3T3-E1 on this modified Ti surface was evaluated by alkaline phosphatase (ALP) activity, mineralization and related osteogenesis genes osteocalcin (OCN), osteopontin (OPN), Collagen-I and Runx2. The result revealed that RA/SH-loaded nano-HAp Ti surface was innocent for cell vitality and even more beneficial for cell osteogenesis in vitro. Furthermore, osteogenesis of MC3T3-E1 showed significant association with the mammalian target of rapamycin (mTOR) phosphorylation by RA, which required further study about the mechanism. The approach to this modified Ti surface presented in this paper has high research value for the development of Ti-based implant.
Department of Oral and Maxillofacial Surgery and Institute of Dental Medicine, Qilu Hospital, Shandong University, Jinan, China.
Chao
C
Liu
School of Stomotology, Shandong University, Jinan, China.
Jian Yong
JY
Dong
School of Stomotology, Shandong University, Jinan, China.
Lin Lin
LL
Yue
Department of Oral and Maxillofacial Surgery and Institute of Dental Medicine, Qilu Hospital, Shandong University, Jinan, China.
Shao Hua
SH
Liu
Key Laboratory of High Efficiency and Clean Mechanical Manufacture Ministry of Education, School of Mechanical Engineering, Shandong University, Jinan, China.
Yi
Y
Wan
State Key Laboratory of Crystal Materials, Center of Bio & Micro/nano Functional Materials, Shandong University, Jinan, PR China.
Hong
H
Liu
Department of Oral and Maxillofacial Surgery and Institute of Dental Medicine, Qilu Hospital, Shandong University, Jinan, China.
Wan Ye
WY
Tan
School of Stomotology, Shandong University, Jinan, China.
Qian Qian
QQ
Guo
Department of Oral and Maxillofacial Surgery and Institute of Dental Medicine, Qilu Hospital, Shandong University, Jinan, China.
Dong
D
Zhang
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology*; D006820:Hyaluronic Acid / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D049329:Nanostructures / Q000737:chemistry; D016348:Osseointegration / Q000187:drug effects; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology; D010012:Osteogenesis / Q000187:drug effects*; D019736:Prostheses and Implants; D020123:Sirolimus / Q000737:chemistry / Q000494:pharmacology*; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology*
D020099:Coated Materials, Biocompatible; D006820:Hyaluronic Acid; D017886:Durapatite; D014025:Titanium; D020123:Sirolimus