Nanoparticle-Mediated X-Ray Radiation Enhancement for Cancer Therapy.
Metallic nanoparticles with a high atomic number release Auger electrons in response to external beam X-ray radiation. When these nanoparticles are selectively delivered to tumors, they have the potential to locally enhance the effects of radiation therapy. Optimizing the therapeutic efficacy of these nanoparticles, however, remains a challenging and time-consuming task. Here we describe three different assays that can be used to experimentally quantify and optimize the in vitro therapeutic efficacy of nanoparticle-mediated X-ray radiation enhancement. These include an IC50 extended dose response curve, clonogenic cell survival assay, and immunoblotting. Collectively, these assays provide information about whether a given nanoparticle provides radiosensitization, the extent of the radiosensitization, and the potential mechanism of radiosensitization.
Department of Chemical Engineering, Northeastern University, Boston, MA, USA.|Nanomedicine Science & Technology Center, Northeastern University, Boston, MA, USA.
Autumn D
AD
Paro
Nanomedicine Science & Technology Center, Northeastern University, Boston, MA, USA.|Department of Physics, Northeastern University, 360 Huntington Ave, Boston, MA, 02115, USA.
Ilanchezhian
I
Shanmugam
Nanomedicine Science & Technology Center, Northeastern University, Boston, MA, USA. a.vandevenmoloney@northeastern.edu.|Department of Physics, Northeastern University, 360 Huntington Ave, Boston, MA, 02115, USA. a.vandevenmoloney@northeastern.edu.
Anne L
AL
van de Ven
D015415:Biomarkers; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D020128:Inhibitory Concentration 50; D053768:Metal Nanoparticles; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism / Q000532:radiotherapy; D011838:Radiation-Sensitizing Agents; D014410:Tumor Stem Cell Assay; D014965:X-Rays
D015415:Biomarkers; D011838:Radiation-Sensitizing Agents
Clonogenic assay; DNA damage; IC50; Immunoblotting; Metallic nanoparticles; Radiosensitization; Surviving fraction