Sanguibacter gelidistatuariae sp. nov., a novel psychrotolerant anaerobe from an ice sculpture in Antarctica, and emendation of descriptions of the family Sanguibacteraceae, the genus Sanguibacter and species S. antarcticus, S. inulinus, S. kedieii, S. marinus, S. soli and S. suarezii.
A novel psychrotolerant bacterium, strain ISLP-3T, was isolated from a sample of naturally formed ice sculpture on the shore of Lake Podprudnoye in Antarctica. Cells were motile, stained Gram-positive, non-spore-forming, straight or slightly curved rods with the shape of a baseball bat. The new isolate was facultatively anaerobic and catalase-positive. Growth occurred at 3-35 °C with an optimum at 22-24 °C, 0-2 % (w/v) NaCl with an optimum at 0.3 % and pH 6.2-9.5 with an optimum at pH 7.5. Strain ISLP-3T grew on several carbon sources, with the best growth on cellobiose. The isolate possessed ureolytic activity but growth was inhibited by urea. The strain was sensitive to: ampicillin, gentamycin, kanamycin rifampicin, tetracycline and chloramphenicol. Major fatty acids were: anteiso-C15 : 0, iso-C16 : 0, C16 : 0, C14 : 0 and iso-C15 : 0. The predominant menaquinone was MK-9(H4). The genomic G+C content was 69.5 mol%. The 16S rRNA gene showed 99 % sequence similarity to that of Sanguibacter suarezii ST-26T, but their recA genes shared ≤91 % sequence similarity, suggesting that this new isolate represents a novel species within the genus Sanguibacter. This conclusion was supported by average nucleotide identity, which was ≤91 % to the most closely related strain. The name Sanguibacter gelidistatuariae sp. nov. is proposed for the novel species with the type strain ISLP-3T=ATCC TSD-17T=DSM 100501T=JCM 30887T). The complete genome draft sequence of ISLP-3T was deposited under IMG OID 2657245272. Emendments to the descriptions of related taxa have been made based on experimental data from our comparative analysis.
Mathematical, Computer and Natural Sciences Department, Athens State University, Athens, AL 35611, USA.
Elena V
EV
Pikuta
Microbiology Department, University of Georgia in Athens, Athens, GA 30602, USA.
Zhe
Z
Lyu
Biology Department, University of Alabama in Huntsville, Huntsville, AL 35899, USA.|Present address: Genome Sequencing Center, HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USA.
Melissa D
MD
Williams
Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK 73019, USA.
Nisha B
NB
Patel
Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
Yuchen
Y
Liu
Mathematical, Computer and Natural Sciences Department, Athens State University, Athens, AL 35611, USA.
Richard B
RB
Hoover
Institut für Mikrobiologie, Veterinärmedizinische Universität Wien, A-1210 Wien, Austria.
Hans-Jürgen
HJ
Busse
Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK 73019, USA.
Paul A
PA
Lawson
Microbiology Department, University of Georgia in Athens, Athens, GA 30602, USA.
William B
WB
Whitman
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000864:Antarctic Regions; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D007053:Ice; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012613:Sculpture; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D007053:Ice; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9