Anti-inflammatory effects of physalin E from Physalis angulata on lipopolysaccharide-stimulated RAW 264.7 cells through inhibition of NF-κB pathway.
Physalin E is a naturally occurring seco-steroid isolated from the stems and aerial parts of Physalis angulata L. (Solanaceae). This study was aimed to explore the anti-inflammatory effects of physalin E on RAW 264.7 mouse macrophages stimulated by lipopolysaccharide (LPS) and the potential underlying mechanisms. The results showed that physalin E significantly inhibited LPS-induced tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) expression and secretion in a dose-dependent manner. Unlike dexamethasone, these effects could not be blocked by miferstone (RU486). Meanwhile, physalin E reduced the degradation of I-kappa B protein in the cytoplasm and downregulated the nuclear factor-κB (NF-κB) p65 protein in the nuclear, which resulted in the inhibition of the NF-κB nuclear translocation. In conclusion, physalin E exerts its anti-inflammatory activities in LPS-induced macrophages. Physalin E can inhibit the production of inflammatory cytokines by targeting the NF-κB signaling pathway.
a Guangdong Food and Drug Vocational College , Guangdong Institute of Chinese Materia Medica , Guangzhou , China.
Yan-Jun
YJ
Yang
b Department of Immunology, Institute of Clinical Pharmacology , Guangzhou University of Chinese Medicine , Guangzhou , China.
Lang
L
Yi
b Department of Immunology, Institute of Clinical Pharmacology , Guangzhou University of Chinese Medicine , Guangzhou , China.
Qing
Q
Wang
b Department of Immunology, Institute of Clinical Pharmacology , Guangzhou University of Chinese Medicine , Guangzhou , China.
Bing-Bing
BB
Xie
b Department of Immunology, Institute of Clinical Pharmacology , Guangzhou University of Chinese Medicine , Guangzhou , China.
Yan
Y
Dong
c Guangdong Provincial Institute of Biological Products and Materia Medica , Guangzhou , China.
Cong-Wei
CW
Sha
D000818:Animals; D015850:Interleukin-6 / Q000276:immunology; D008070:Lipopolysaccharides / Q000633:toxicity*; D051379:Mice; D016328:NF-kappa B / Q000276:immunology*; D032321:Physalis / Q000737:chemistry*; D000067996:RAW 264.7 Cells; D012632:Secosteroids / Q000737:chemistry / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects* / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
D015850:Interleukin-6; D008070:Lipopolysaccharides; D016328:NF-kappa B; D012632:Secosteroids; D014409:Tumor Necrosis Factor-alpha; C577686:interleukin-6, mouse; C553897:physalin E
Physalin E; cytokine; glucocorticoid receptor; lipopolysaccharide; macrophage; nuclear factor-κB