Genome Sequence of Microbulbifer mangrovi DD-13TReveals Its Versatility to Degrade Multiple Polysaccharides.
Microbulbifer mangrovi strain DD-13Tis a novel-type species isolated from the mangroves of Goa, India. The draft genome sequence of strain DD-13 comprised 4,528,106 bp with G+C content of 57.15%. Out of 3479 open reading frames, functions for 3488 protein coding sequences were predicted on the basis of similarity with the cluster of orthologous groups. In addition to protein coding sequences, 34 tRNA genes and 3 rRNA genes were detected. Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs). Many genes from strain DD-13 were annotated as carbohydrases specific for degradation of agar, alginate, carrageenan, chitin, xylan, pullulan, cellulose, starch, β-glucan, pectin, etc. Some of polysaccharide-degrading genes were highly modular and were appended at least with one CBM indicating the versatility of strain DD-13 to degrade complex polysaccharides. The cell growth of strain DD-13 was validated using pure polysaccharides such as agarose or alginate as carbon source as well as by using red and brown seaweed powder as substrate. The homologous carbohydrase produced by strain DD-13 during growth degraded the polysaccharide, ensuring the production of metabolizable reducing sugars. Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
Department of Biotechnology, Goa University, Taleigao Plateau, Goa, 403206, India.
Md
M
Imran
Plant Molecular Biology Laboratory, CSIR-National Botanical Research Institute, Lucknow, 226001, India.
Poonam
P
Pant
Department of Biotechnology, Goa University, Taleigao Plateau, Goa, 403206, India.
Yogini P
YP
Shanbhag
Plant Molecular Biology Laboratory, CSIR-National Botanical Research Institute, Lucknow, 226001, India.
Samir V
SV
Sawant
Department of Biotechnology, Goa University, Taleigao Plateau, Goa, 403206, India. saga@unigoa.ac.in.
Sanjeev C
SC
Ghadi
D001426:Bacterial Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D001482:Base Composition; D001673:Biodegradation, Environmental; D003470:Culture Media; D020563:Gammaproteobacteria / Q000201:enzymology / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D063990:Gene Ontology; D059646:Genome Size; D016680:Genome, Bacterial; D006026:Glycoside Hydrolases / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D006868:Hydrolysis; D058977:Molecular Sequence Annotation; D016366:Open Reading Frames; D011134:Polysaccharides / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D012624:Seaweed / Q000737:chemistry
D001426:Bacterial Proteins; D003470:Culture Media; D011134:Polysaccharides; D011956:Receptors, Cell Surface; C055213:saccharide-binding proteins; D006026:Glycoside Hydrolases
Agarase; Alginate lyase; Carbohydrate-binding module; Glycoside hydrolases; Microbulbifer mangrovi