Are Entomopathogenic Nematodes Effective Biological Control Agents Against the Carob Moth,Ectomyelois ceratoniae?
The carob moth (Ectomyelois ceratoniae) is the key pest of pomegranate, which causes a significant percentage of losses in pomegranate orchards and warehouses of Iran annually. The pest larvae are characterized by displaying a cryptic behavior within the fruit, which avoids most routine control techniques, especially chemical method. The low efficiency of traditional measurements and also the rich species diversity of natural enemies within the infested fruits highlight the necessity of exploring effective control methods, especially environmental friendly approaches. Entomopathogenic nematodes (EPNs) are a group of biological control agents that actively search for the host, including those in a cryptic habitat like the carob moth larvae within infested fruits. Here, we assumed that treatment of the infested and dropped fruits with EPNs may provide new insight into the management of the carob moth. Three species of EPNs,Steinernema feltiae,S. carpocapsae, andHeterorhabditis bacteriophorawere selected and used in a series of in vitro and in vivo experiments. In preliminary assays, the EPNs species were used with different concentrations of infective juveniles (IJs) (0, 1, 5, 10, 25, and 50 IJ/larvae) in 2-cm diam. plates. The mortality rates of the laboratory tests were 79.75% and 76.5% forS. feltiaeandS. carpocapsae, corresponded to LC50value of 2.02 IJ/larva forS. feltiaeand 2.05 IJ/larva forS. carpocapsae. On the contrary,H. bacteriophorademonstrated low virulence on the pest larvae in petri tests with a LC50= 426.92 IJ/larva. Hence, bothSteinernemaspecies were selected for subsequent experiments. The penetration rate forS. feltiaeandS. carpocapsaeinto the hemocoel of the pest was 43% and 31%, respectively, and the corresponding reproduction rate was 15,452 IJ/larva forS. feltiaeand 18,456 IJ/larva forS. carpocapsae. The gathered data from those in vitro tests were used for a field assay. Different concentrations (5, 10, 50, 100, and 160 IJ/cm2of the arena) ofS. feltiaeandS. carpocapsaewere applied in the field test. The mean mortality results from the last test were 10.89% and 26.65% forS. feltiaeandS. carpocapsae, respectively. Finally, we found that these low virulence rates of the nematodes were attributed to inhibitory/repellency effects of saprophytic fungi within the infested pomegranates, a usual status of the infested fruits in autumn or winter seasons. Future work on additional EPN populations more adapted to the extreme conditions of the pomegranate production area in Iran may provide sufficient evidence to continue the further investigation on the best EPN species populations and advanced formulations with high durability.
Biocontrol and Insect Pathology Laboratory, Department of Plant Protection, School of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Zahra
Z
Memari
Biocontrol and Insect Pathology Laboratory, Department of Plant Protection, School of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Javad
J
Karimi
Biocontrol and Insect Pathology Laboratory, Department of Plant Protection, School of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Shokoofeh
S
Kamali
College of Agriculture and Natural Resources, University of Tehran, Karaj Campus, Iran.
Seyed Hossein
SH
Goldansaz
Biocontrol and Insect Pathology Laboratory, Department of Plant Protection, School of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Mojtaba
M
Hosseini
biological control; entomopathogenic nematode; insect pathology; pathogenicity; pomegranate moth