Impact of Air Pollution to Genome of Newborns.
The Northern Moravia Region is the most polluted region in the Czech Republic by particulate matter (PM2.5) and carcinogenic polycyclic aromatic hydrocarbons (c-PAHs) as benzo[a]pyrene (B[a]P) by heavy industry and local heating. This specific situation was used to study the impact of air pollution on newborns in the exposed Karviná district and control district of České Budějovice. Biological material from newborns and mothers was collected in summer and winter seasons. This project is highly detailed, analyzing the concentrations of PAHs in ambient air and diet, in human breast milk, in the urine of mothers and newborns, using biomarkers of genetic damage as DNA adducts and gene expression analysis, biomarkers of oxidative stress as 8-oxodG adducts and lipid peroxidation (15-F2t-isoprostane immunoassay). All 400 children, for whom the biomarker data at delivery were obtained, will be followed for morbidity up to 2 years of age. The Northern Moravia Region seems to be to be a model area for studying the long-term impact of human health exposure to c-PAHs. Our observations will indicate possible genetic and oxidative damage in newborns, which may significantly affect their morbidity.
Institute of Experimental Medicine AS CR, Prague, Czech Republic.
Radim J
RJ
Šrám
Institute of Experimental Medicine AS CR, Prague, Czech Republic.
Pavel
P
Rössner
Institute of Experimental Medicine AS CR, Prague, Czech Republic.
Andrea
A
Rössnerová
Institute of Experimental Medicine AS CR, Prague, Czech Republic.
Miroslav
M
Dostál
Institute of Experimental Medicine AS CR, Prague, Czech Republic.
Alena
A
Milcová
Institute of Experimental Medicine AS CR, Prague, Czech Republic.
Vlasta
V
Švecová
University of Chemistry and Technology, Prague, Czech Republic.
Jana
J
Pulkrabová
University of Chemistry and Technology, Prague, Czech Republic.
Jana
J
Hajšlová
University of South Bohemia, České Budějovice, Czech Republic.
Miloš
M
Velemínský
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000393:Air Pollutants / Q000032:analysis*; D001564:Benzo(a)pyrene / Q000032:analysis; D018153:Czech Republic; D018736:DNA Adducts; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis; D004032:Diet; D004781:Environmental Exposure / Q000032:analysis*; D004784:Environmental Monitoring; D005260:Female; D016678:Genome; D006801:Humans; D007221:Industry; D007231:Infant, Newborn; D008297:Male; D008895:Milk, Human / Q000737:chemistry; D018384:Oxidative Stress; D052638:Particulate Matter / Q000032:analysis; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D011247:Pregnancy; D060888:Real-Time Polymerase Chain Reaction; D012621:Seasons
D000393:Air Pollutants; D018736:DNA Adducts; D052638:Particulate Matter; D011084:Polycyclic Aromatic Hydrocarbons; D001564:Benzo(a)pyrene; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine
air pollution; diet; genetic damage; molecular epidemiology; newborns; oxidative stress; polycyclic aromatic hydrocarbons