Lipid-load in peripheral blood mononuclear cells: Impact of food-consumption, dietary-macronutrients, extracellular lipid availability and demographic factors.
Lipid-load in peripheral blood mononuclear cells (PBMCs) has recently gained attention of the researchers working on nutritional regulation of metabolic health. Previous works have indicated that the metabolic circuitries in the circulating PBMCs are influenced by dietary-intake and macronutrient composition of diet. In the present work, we analyzed the impact of diet and dietary macronutrients on PBMCs' lipid-load. The overall analyses revealed that dietary carbohydrates and fats combinatorially induce triglyceride accumulation in PBMCs. On the other hand, dietary fats were shown to induce significant decrease in PBMCs' cholesterol-load. The effects of various demographic factors -including age, gender and body-weight- on PBMCs' lipid-load were also examined. Body-weight and age were both shown to affect PBMC's lipid-load. Our study fails to provide any direct association between extracellular lipid availability and cholesterol-load in both, freshly isolated and cultured PBMCs. The presented work significantly contributes to the current understanding of the impact of food-consumption, dietary macronutrients, extracellular lipid availability and demographic factors on lipid-load in PBMCs.
Microbiology and Molecular Genetics, University of the Punjab, Lahore, 54590, Pakistan.
Fatima
F
Ameer
Microbiology and Molecular Genetics, University of the Punjab, Lahore, 54590, Pakistan.
Rimsha
R
Munir
Microbiology and Molecular Genetics, University of the Punjab, Lahore, 54590, Pakistan.
Hina
H
Usman
Microbiology and Molecular Genetics, University of the Punjab, Lahore, 54590, Pakistan.
Rida
R
Rashid
Microbiology and Molecular Genetics, University of the Punjab, Lahore, 54590, Pakistan.
Muhammad
M
Shahjahan
Microbiology and Molecular Genetics, University of the Punjab, Lahore, 54590, Pakistan.
Shahida
S
Hasnain
Microbiology and Molecular Genetics, University of the Punjab, Lahore, 54590, Pakistan. Electronic address: nzzaidi@yahoo.com.
Nousheen
N
Zaidi
D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism; D004040:Dietary Carbohydrates / Q000378:metabolism; D004041:Dietary Fats / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008055:Lipids; D014280:Triglycerides / Q000378:metabolism
D004040:Dietary Carbohydrates; D004041:Dietary Fats; D008055:Lipids; D014280:Triglycerides; D002784:Cholesterol
Cellular lipids; Dietary macronutrients; Peripheral blood mononuclear cells