Turn-on fluorescence detection of ascorbic acid with gold nanolcusters.
In this report, a sensitive fluorescent detection of ascorbic acid was developed with protein-stabilized gold nanoclusters. The fluorescence signal from the gold nanoclusters could be effectively quenched by elemental iodine. This kind of quenching could be inhibited by ascorbic acid through a corresponding reduction process, thus a turn-on response toward ascorbic acid was obtained. The validity and performances of the detection scheme were evaluated. The fluorescence responses were linearly related to ascorbic acid in the concentration range 0.1-10μM, and the method was capable of detecting ascorbic acid down to 22nM. The high performances of the developed scheme greatly simplified the sample preparation step, and the method was successfully applied for real sample analyses.
The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Road, Industrial Park, Suzhou 215123, China.
Huijie
H
Meng
The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Road, Industrial Park, Suzhou 215123, China.
Dongqin
D
Yang
The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Road, Industrial Park, Suzhou 215123, China.
Yifeng
Y
Tu
The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Road, Industrial Park, Suzhou 215123, China. Electronic address: yanjl@suda.edu.cn.
Jilin
J
Yan
D001205:Ascorbic Acid / Q000032:analysis*; D001628:Beverages / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D005453:Fluorescence; D005638:Fruit / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D006046:Gold; D001205:Ascorbic Acid
Antioxidant; Ascorbic acid; Fluorescence; Gold nanoclusters; Iodine