A Validated HPLC Method for Simultaneous Determination of Perindopril Arginine, Amlodipine, and Indapamide: Application in Bulk and in Different Pharmaceutical Dosage Forms.
A simple, accurate, and precise LC method with a reversed stationary phase was developed and validated for the determination of perindopril (PER) arginine, amlodipine (AML), and indapamide (IND) alone and in binary mixtures (PER arginine is found in two dosage forms, i.e., with either AML or IND). Chromatographic separation was carried out on a BDS Hypersil® C18 column (100 × 3 mm, 5 μm). The mobile phase, consisting of 0.05 M potassium dihydrogen phosphate buffer (pH 2.6)-methanol (50 + 50, v/v), was pumped through the column whose temperature was maintained at 50°C at a flow rate of 0.6 mL/min using isocratic elution, and UV detection at 215 nm was performed. Acceptable values of linearity, accuracy, and precision of the method were found over the concentration ranges of 5-80 μg/mL PER, 2.5-80 μg/mL AML, and 0.5-20 μg/mL IND. The proposed chromatographic method was statistically compared to that of reference methods using one-way analysis of variance. The results showed that there was no significant difference between the methods. The developed method proved reliable for use in accurate QC of the drugs in their pharmaceutical preparations.
Cairo University, Faculty of Pharmacy, Pharmaceutical Chemistry Department, Kasr El-Aini St, Cairo 11562, Egypt.
Ramzia I
RI
El-Bagary
Cairo University, Faculty of Pharmacy, Pharmaceutical Chemistry Department, Kasr El-Aini St, Cairo 11562, Egypt.
Ehab F
EF
Elkady
British University in Egypt, Faculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, Sweis Rd, El-Sherouk City, Cairo 11837, Egypt.
Shereen
S
Mowaka
British University in Egypt, Faculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, Sweis Rd, El-Sherouk City, Cairo 11837, Egypt.
Maria A
MA
Attallah
D017311:Amlodipine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D007190:Indapamide / Q000032:analysis*; D020913:Perindopril / Q000032:analysis*
D017311:Amlodipine; D007190:Indapamide; D020913:Perindopril