Antimicrobial Activities of Phenolic Extracts Derived from Seed Coats of Selected Soybean Varieties.
Soybean hulls or seed coats consist of complex carbohydrates, proteins, lipids, and polyphenols such as anthocyanidins, proanthocyanidins, and isoflavones. The polyphenolics in the seed coats give them various colors such as black, brown, green, yellow, or even a mottled appearance. In this study, the antimicrobial effects of phenolic extracts from the seed coats of different colored soybeans (yellow, dark brown, brown, and black) were evaluated against foodborne pathogens such as Salmonella Typhimurium, Escherichia coli O157:H7, and Campylobacter jejuni in broth-cultures as well as on chicken skin. The highest total phenolic content was observed for the phenolic extract from soybean variety (R07-1927) with black colored seed coat (74.1 ± 2.1 mg chlorogenic acid equivalent [CAE]/g extract) and was significantly different (P <0.0001) from the extract of the conventional soybean variety (R08-4004) with yellow colored seed coat (7.4 ± 1.2 mg CAE/g extract). The extract from black colored soybean produced reductions of 2.10 ± 0.08 to 2.20 ± 0.08-log CFU/mL for both E. coli O157:H7 and C. jejuni after 3 d when incubated in broth-culture having 4-log CFU/mL of bacteria, whereas a 6 d incubation was found to reduce S. Typhimurium and E. coli O157:H7 at 2.03 ± 0.05 and 3.3 ± 0.08-log CFU/mL, respectively. The extract also reduced S. Typhimurium and E. coli O157:H7 attached to chicken skin by 1.39 ± 0.03 and 1.24 ± 0.06-log CFU/g, respectively, upon incubation for 6 d. Soybean seed coat extracts may have a potency as antimicrobial agents to reduce foodborne bacteria contaminating poultry products.
Dept. of Food Science, Univ. of Arkansas, Fayetteville, AR, 72704, U.S.A.
Rajaa
R
Abutheraa
Dept. of Food Science, Univ. of Arkansas, Fayetteville, AR, 72704, U.S.A.
Navam
N
Hettiarachchy
Dept. of Poultry Science, Univ. of Arkansas, Fayetteville, AR, 72701, U.S.A.
Geetha
G
Kumar-Phillips
Dept. of Food Science, Univ. of Arkansas, Fayetteville, AR, 72704, U.S.A.
Ronny
R
Horax
Dept. of Crop, Soil, and Environmental Sciences, Univ. of Arkansas, Fayetteville, AR, 72701, U.S.A.
Pengyin
P
Chen
Dept. of Food Science, Univ. of Arkansas, Fayetteville, AR, 72704, U.S.A.
Ruben
R
Morawicki
Dept. of Poultry Science, Univ. of Arkansas, Fayetteville, AR, 72701, U.S.A.
Young Min
YM
Kwon
D000818:Animals; D000872:Anthocyanins / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D016123:Campylobacter jejuni / Q000187:drug effects; D002645:Chickens; D015169:Colony Count, Microbial; D019453:Escherichia coli O157 / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology*; D005516:Food Microbiology; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology; D008460:Meat / Q000382:microbiology; D010636:Phenols / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D059808:Polyphenols / Q000494:pharmacology; D044945:Proanthocyanidins / Q000494:pharmacology; D012486:Salmonella typhimurium / Q000187:drug effects; D012639:Seeds / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry* / Q000145:classification; D013045:Species Specificity
D000872:Anthocyanins; D000890:Anti-Infective Agents; D005419:Flavonoids; D007529:Isoflavones; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D044945:Proanthocyanidins
antibacterial activity; chicken; foodborne pathogens; phenolics; soybean seed coats