Transcriptome profiling of the Australian arid-land plant Eremophila serrulata (A.DC.) Druce (Scrophulariaceae) for the identification of monoterpene synthases.
Plant terpenoids are a large and highly diverse class of metabolites with an important role in the immune defense. They find wide industrial application as active pharmaceutical ingredients, aroma and fragrance compounds. Several Eremophila sp. derived terpenoids have been documented. To elucidate the terpenoid metabolism, the transcriptome of juvenile and mature Eremophila serrulata (A.DC.) Druce (Scrophulariaceae) leaves was sequenced and a transcript library was generated. We report on the first transcriptomic dataset of an Eremophila plant. IlluminaMiSeq sequencing (2 × 300 bp) revealed 7,093,266 paired reads, which could be assembled to 34,505 isogroups. To enable detection of terpene biosynthetic genes, leaves were separately treated with methyl jasmonate, a well-documented inducer of plant secondary metabolites. In total, 21 putative terpene synthase genes were detected in the transcriptome data. Two terpene synthase isoenzymatic genes, termed ES01 and ES02, were successfully expressed in E. coli. The resulting proteins catalyzed the conversion of geranyl pyrophosphate, the universal substrate of monoterpene synthases to myrcene and Z-(b)-ocimene, respectively. The transcriptomic data and the discovery of the first terpene synthases from Eremophila serrulata are the initial step for the understanding of the terpene metabolism in this medicinally important plant genus.
Junior Research Group for Microbial Biotechnology, Ruhr-Universität Bochum, Universitätsstraße 150, 44780 Bochum, Germany.
Octavia Natascha
ON
Kracht
Junior Research Group for Microbial Biotechnology, Ruhr-Universität Bochum, Universitätsstraße 150, 44780 Bochum, Germany.
Ann-Christin
AC
Ammann
Junior Research Group for Microbial Biotechnology, Ruhr-Universität Bochum, Universitätsstraße 150, 44780 Bochum, Germany.
Julia
J
Stockmann
Centre for Biotechnology, University of Bielefeld, 33615 Bielefeld, Germany.
Daniel
D
Wibberg
Centre for Biotechnology, University of Bielefeld, 33615 Bielefeld, Germany.
Jörn
J
Kalinowski
Chair of Plant Physiology, Ruhr-Universität Bochum, Universitätsstraße 150, 44780 Bochum, Germany.
Markus
M
Piotrowski
Marine Natural Products Lab, University of Prince Edward Island, 550 University Avenue, Charlottetown, PEI, Canada.
Russell
R
Kerr
Chair of Industrial Biocatalysis, Technical University of Munich, Lichtenbergstraße 4, 85748 Garching, Germany.
Thomas
T
Brück
Junior Research Group for Microbial Biotechnology, Ruhr-Universität Bochum, Universitätsstraße 150, 44780 Bochum, Germany. Electronic address: kourist@tugraz.at.
Robert
R
Kourist
D000085:Acetates; D000080462:Acyclic Monoterpenes; D000475:Alkenes; D019883:Alkyl and Aryl Transferases / Q000378:metabolism*; D001315:Australia; D003517:Cyclopentanes; D041621:Eremophila Plant; D004926:Escherichia coli / Q000235:genetics; D020869:Gene Expression Profiling; D019753:Intramolecular Lyases / Q000378:metabolism; D039821:Monoterpenes; D054883:Oxylipins; D010940:Plant Proteins / Q000378:metabolism; D011106:Polyisoprenyl Phosphates / Q000378:metabolism; D028083:Scrophulariaceae / Q000235:genetics; D013729:Terpenes / Q000378:metabolism*
D000085:Acetates; D000080462:Acyclic Monoterpenes; D000475:Alkenes; D003517:Cyclopentanes; D039821:Monoterpenes; D054883:Oxylipins; D010940:Plant Proteins; D011106:Polyisoprenyl Phosphates; D013729:Terpenes; C509595:myrcene; C015234:geranyl pyrophosphate; C072239:methyl jasmonate; D019883:Alkyl and Aryl Transferases; C108877:terpene synthase; D019753:Intramolecular Lyases; C040265:pinene cyclase I
Eremophila serrulata (A.DC.) Druce; Geranyl pyrophosphate; Isoenzymes; Myrcene synthase; Ocimene synthase; Scrophulariaceae; Terpene synthase