Prions.
Infectious proteins (prions) are usually self-templating filamentous protein polymers (amyloids). Yeast prions are genes composed of protein and, like the multiple alleles of DNA-based genes, can have an array of "variants," each a distinct self-propagating amyloid conformation. Like the lethal mammalian prions and amyloid diseases, yeast prions may be lethal, or only mildly detrimental, and show an array of phenotypes depending on the protein involved and the prion variant. Yeast prions are models for both rare mammalian prion diseases and for several very common amyloidoses such as Alzheimer's disease, type 2 diabetes, and Parkinson's disease. Here, we describe their detection and characterization using genetic, cell biological, biochemical, and physical methods.
Department of Pharmacology, Uniformed Services University of Health Sciences, Bethesda, Maryland 20814.
Dmitry
D
Kryndushkin
Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0830.
Herman K
HK
Edskes
Department of Pharmacology, Uniformed Services University of Health Sciences, Bethesda, Maryland 20814.
Frank P
FP
Shewmaker
Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0830 wickner@helix.nih.gov.
Reed B
RB
Wickner
D000682:Amyloid / Q000235:genetics* / Q000378:metabolism*; D008954:Models, Biological; D011328:Prions / Q000235:genetics* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*
D000682:Amyloid; D011328:Prions; D029701:Saccharomyces cerevisiae Proteins