Ethylenediaminetetraacetic Acid, Sodium Citrate, Heparin and Citrate Phosphate Dextrose-Adenine Anticoagulants Differentially Affect Cytokine mRNA Expression in Blood Leukocytes.
BACKGROUND
The aim of this study is to determine whether and how the anticoagulant agents EDTA, sodium citrate, and heparin present in vacutainers and CPDA located in transfusion bags modify peripheral blood cell behavior.


METHODS
We compared the effect of four anticoagulants within an expression study quantifying mRNA by realtime PCR in 60 blood samples.


RESULTS
We observed a significant increase of TNFα and CCL2 mRNA expression in leukocytes placed in EDTA anticoagulant compared to leukocytes collected with citrate or heparin (p ≤ 0.5). Compared to blood cells influenced by one of the three anticoagulant agents present in vacuum tubes, cells from transfusion bags affected by CPDA displayed significantly lower levels of CCL2 and MYC mRNA, whereas levels of IL-10 and TNFα mRNA were much higher (p ≤ 0.5).


CONCLUSIONS
The anticoagulant EDTA significantly increases proinflammatory cytokine mRNA in freshly isolated PBMC. Anticoagulant CPDA significantly alters levels of some of the tested mRNAs in comparison to blood cells drawn into one of the three anticoagulant agents present in vacutainers.
Eva
E
Herzogova
Pavlina
P
Dankova
D000225:Adenine / Q000494:pharmacology; D000925:Anticoagulants / Q000494:pharmacology*; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism*; D002951:Citrates / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D000077559:Sodium Citrate; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
D000925:Anticoagulants; D018932:Chemokine CCL2; D002951:Citrates; C489427:MYC protein, human; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D000077559:Sodium Citrate; C007077:citrate phosphate dextrose; D006493:Heparin; D004492:Edetic Acid; D005947:Glucose; D000225:Adenine