Astragaloside IV ameliorates 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis implicating regulation of energy metabolism.
Dysfunction of energy metabolism is involved in inflammatory bowel disease (IBD). This study was designed to investigate the potential of astragaloside IV (ASIV), an active ingredient of Radix Astragalus, to ameliorate colonic mucosal injury, with focusing on the implication of energy restoration in the underlying mechanism. Experimental colitis model was established in rats by injecting 2,4,6-trinitrobenzene sulfonic acid (TNBS) through anus. After 24 hours, ASIV was administrated once daily by gavage for 6 days. On day 1 and day 7, colon tissue was collected for macroscopic and histological examination, ELISA, Western blot and immunohistochemical analysis. TNBS impaired colonic mucosa with an injured epithelial architecture, increased inflammatory cell infiltration, and decreased colonic blood flow. Lgr5 positive cell number in crypt and β-catenin nuclear translocation were down-regulated by TNBS treatment. TNBS induced epithelial F-actin disruption and junctional protein degradation. Furthermore, adenosine triphosphate (ATP) content and ATP synthase subunit β expression in the colon tissue were significantly decreased after TNBS stimulation. All of the aforementioned alterations were relieved by ASIV post-treatment. The present study revealed that ASIV promoted mucosal healing process in TNBS-induced colitis, which was most likely attributed to regulating energy metabolism.
Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China.|Shandong college of Traditional Chinese Medicine, Yantai, Shandong, China.
Xu-Guang
XG
Jiang
Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China.|Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine, Beijing 100191, China.|Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing 100191, China.
Kai
K
Sun
Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine, Beijing 100191, China.|Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing 100191, China.
Yu-Ying
YY
Liu
Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine, Beijing 100191, China.|Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing 100191, China.
Li
L
Yan
Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine, Beijing 100191, China.|Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing 100191, China.
Ming-Xia
MX
Wang
Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine, Beijing 100191, China.|Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing 100191, China.
Jing-Yu
JY
Fan
Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine, Beijing 100191, China.|Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing 100191, China.
Hong-Na
HN
Mu
Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine, Beijing 100191, China.|Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing 100191, China.
Chong
C
Li
Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine, Beijing 100191, China.|Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing 100191, China.
Yuan-Yuan
YY
Chen
Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China.|Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine, Beijing 100191, China.|Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing 100191, China.
Chuan-She
CS
Wang
Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China.|Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine, Beijing 100191, China.|Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing 100191, China.
Jing-Yan
JY
Han
D000199:Actins / Q000378:metabolism; D000818:Animals; D015415:Biomarkers; D002452:Cell Count; D003092:Colitis / Q000188:drug therapy / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology; D003106:Colon / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism / Q000187:drug effects*; D007150:Immunohistochemistry; D015212:Inflammatory Bowel Diseases / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D059748:Proteolysis / Q000187:drug effects; D051381:Rats; D012039:Regional Blood Flow / Q000187:drug effects; D012503:Saponins / Q000737:chemistry / Q000494:pharmacology*; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D062725:Tight Junction Proteins / Q000378:metabolism; D014302:Trinitrobenzenesulfonic Acid / Q000009:adverse effects*; D014315:Triterpenes / Q000737:chemistry / Q000494:pharmacology*
D000199:Actins; D015415:Biomarkers; D012503:Saponins; D062725:Tight Junction Proteins; D014315:Triterpenes; C052064:astragaloside A; D014302:Trinitrobenzenesulfonic Acid