Transepithelial transport efficiency of bovine collagen hydrolysates in a human Caco-2 cell line model.
Collagen was extracted from raw bovine hide and hydrolyzed by one of three enzymes - Alcalase, Flavourzyme, or trypsin - or by using a combination of two or three of these enzymes. The Alcalase-containing enzymatic hydrolysis treatments generated a greater proportion of hydrolysates with molecular weight (MW) <2kDa (79.8-82.7%). Flavourzyme-containing hydrolysis treatments exhibited the greatest proportion of free amino acids (686-740nmol/mg). The hydrolysates were then subjected to a simulated gastrointestinal (GI) digestion, and transport studies were conducted using a Caco-2 cell model. Due to the lower MW profile, the hydrolyzed collagen showed greater resistance to GI digestion and greater transport efficiency than the unhydrolyzed collagen control. Hydrolysates from a dual enzyme mixture - the Alcalase/Flavourzyme combination - generated the greatest transport efficiency across Caco-2 cell monolayers (21.4%), two-fold more than that of the collagen control.
Department of Agricultural, Food and Nutritional Sciences, University of Alberta, Canada. Electronic address: mfeng3@ualberta.ca.
Mengmeng
M
Feng
Department of Agricultural, Food and Nutritional Sciences, University of Alberta, Canada. Electronic address: mirko.betti@ualberta.ca.
Mirko
M
Betti
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D018938:Caco-2 Cells; D002417:Cattle; D003094:Collagen / Q000378:metabolism*; D004063:Digestion; D010450:Endopeptidases / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D008970:Molecular Weight; D013381:Subtilisins / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
D000596:Amino Acids; D003094:Collagen; D010450:Endopeptidases; C412470:flavourzyme; D013381:Subtilisins; D014357:Trypsin
Alcalase; Bioavailability; Bovine collagen hydrolysates; Caco-2 cell; Flavourzyme; Low molecular weight peptides; Trypsin