Extraction optimization and in vitro and in vivo anti-postprandial hyperglycemia effects of inhibitor from Phoenix dactylifera L. parthenocarpic fruit.
Phoenix dactylifera L. plays an important role in social, economic, and ecological Tunisian sectors. Some date palms produce parthenocarpic fruit named Sish. The aqueous ethanolic extract from P. dactylifera parthenocarpic dates demonstrated a potent inhibition of the enzymes related to type II diabetes. In this work, extraction optimization of amylase inhibitors was carried out using Box-Behnken Design. Bioactivity-guided fractionation of the 70% aqueous ethanol extract was performed to identify the active compounds. The physicochemical results by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis showed the presence of 13 phenolic compounds. The in vitro study showed that the extract exhibited a more specific inhibitor of α-glucosidase than α-amylase with an IC50value of 0.6 and 2.5mg/mL, respectively. The in vivo study of this extract effect on the postprandial hyperglycemia activity showed a decrease in plasma glucose levels after 30min stronger than the Acarbose effect. These results confirmed the anti-postprandial hyperglycemia activity of the aqueous ethanolic extract from P. dactylifera parthenocarpic dates, which could lend support for its pharmaceutical use.
Laboratory of Plant Biotechnology, Sfax Faculty of Sciences, PB 1171, University of Sfax, 3038 Sfax, Tunisia. Electronic address: hanenelabed566@hotmail.fr.
Hanen
H
El Abed
Laboratory of Plant Biotechnology, Sfax Faculty of Sciences, PB 1171, University of Sfax, 3038 Sfax, Tunisia.
Mouna
M
Chakroun
Laboratory of Toxicology, Environmental Microbiology and Health, Sfax Faculty of Sciences, PB 1171, University of Sfax, 3038 Sfax, Tunisia.
Imen
I
Fendri
Laboratory of Toxicology, Environmental Microbiology and Health, Sfax Faculty of Sciences, PB 1171, University of Sfax, 3038 Sfax, Tunisia.
Mohamed
M
Makni
Electro-Chemical Environmental Laboratory, ENIS, BP 1173 Soukra Road, University of Sfax, Sfax 3038, Tunisia.
Mohamed
M
Bouaziz
Laboratory of Plant Biotechnology, Sfax Faculty of Sciences, PB 1171, University of Sfax, 3038 Sfax, Tunisia.
Noureddine
N
Drira
Laboratory of Plant Biotechnology, Sfax Faculty of Sciences, PB 1171, University of Sfax, 3038 Sfax, Tunisia.
Hafedh
H
Mejdoub
Laboratory of Plant Biotechnology, Sfax Faculty of Sciences, PB 1171, University of Sfax, 3038 Sfax, Tunisia.
Bassem
B
Khemakhem
D020909:Acarbose / Q000494:pharmacology; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry*; D005951:Glucose Tolerance Test; D006943:Hyperglycemia / Q000517:prevention & control*; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D008297:Male; D051379:Mice; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology; D065749:Phoeniceae / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D013634:Tannins / Q000032:analysis / Q000494:pharmacology; D000516:alpha-Amylases / Q000037:antagonists & inhibitors; D000520:alpha-Glucosidases / Q000378:metabolism
D001786:Blood Glucose; D005419:Flavonoids; D007004:Hypoglycemic Agents; D010636:Phenols; D010936:Plant Extracts; D013634:Tannins; D000516:alpha-Amylases; D000520:alpha-Glucosidases; D020909:Acarbose
Box-Behnken design; Glucosyl hydrolase; Hyperglycemia; Inhibitor; LC–MS/MS analysis; Parthenocarpic dates