Molecular and antimicrobial susceptibility characterization of Globicatella sulfidifaciens isolated from sow's urinary tract infection.
BACKGROUND
The Globicatella genus comprises Gram-positive, facultative anaerobic, α-hemolytic and catalase negative cocci morphologically and phenotypically very similar to Streptococcus and Aerococcus genus which can lead to misidentification and underestimation of this pathogen. Globicatella species have already been isolated from human and animals with heart and brain disorders. Their clinical relevance in animals, and its zoonotic potential, remains unknown due to the difficulty in their identification.


OBJECTIVE
To present the isolation, phenotypic and molecular characterization of G. sulfidifaciens from urinary tract infection in sows.


METHODS
Urine samples from 140 sows of two swine herds located in São Paulo State (Brazil) yielded the isolation of three presumptive G. sulfidifaciens strains. Identification and species confirmation were done by MALDI-TOF MS and 16S rRNA sequencing. Strains were further characterized by single enzyme amplified fragments length polymorphism (SE-AFLP) and broth microdilution techniques.


RESULTS
All three isolates were confirmed as G. sulfidifaciens. The SE-AFLP genotyping resulted in distinct fingerprint patterns for each strain. All isolates presented high MIC values to tetracycline, sulphonamides, aminoglycosides and tylosin tartrate, which present high usage in human and animal medicine.


CONCLUSIONS
Globicatella sulfidifaciens could be related to sporadic urinary tract infections in swine and appear to present alarming antimicrobial susceptibility profile. It is necessary to differentiate Streptococcus-like microorganisms in routine laboratory diagnostics for the correct identification of underestimated species potentially pathogenic to animals.
a Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia , Universidade de São Paulo , São Paulo , Brazil.
Carlos E C
CE
Matajira
a Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia , Universidade de São Paulo , São Paulo , Brazil.
Luisa Z
LZ
Moreno
a Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia , Universidade de São Paulo , São Paulo , Brazil.
Vasco T M
VT
Gomes
a Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia , Universidade de São Paulo , São Paulo , Brazil.
Ana Paula S
AP
Silva
a Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia , Universidade de São Paulo , São Paulo , Brazil.
Renan E
RE
Mesquita
a Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia , Universidade de São Paulo , São Paulo , Brazil.
Cristina R
CR
Amigo
b Companhia Ambiental do Estado de São Paulo (CETESB) , São Paulo , Brazil.
Ana Paula G
AP
Christ
b Companhia Ambiental do Estado de São Paulo (CETESB) , São Paulo , Brazil.
Maria Inês Z
MI
Sato
a Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia , Universidade de São Paulo , São Paulo , Brazil.
Andrea M
AM
Moreno
D056567:Aerococcaceae / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification; D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001938:Brazil; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D016908:Gram-Positive Bacterial Infections / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D008826:Microbial Sensitivity Tests / Q000662:veterinary*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000188:drug therapy* / Q000382:microbiology; D014552:Urinary Tract Infections / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
Globicatella sulfidifaciens; MIC; Porcine; sequencing; sow; urinary tract infection