CasuL: A new lectin isolated from Calliandra surinamensis leaf pinnulae with cytotoxicity to cancer cells, antimicrobial activity and antibiofilm effect.
This work describes the isolation of a lectin (CasuL) from the leaf pinnulae of Calliandra surinamensis and the evaluation of its cytotoxic, antimicrobial and antibiofilm properties. Proteins from pinnulae extract were precipitated with ammonium sulphate (60% saturation) and submitted to Sephadex G-75 chromatography, which yielded isolated CasuL (purification factor: 113). Native CasuL is an acidic protein (pI 5.82) with a relative molecular mass of 48kDa. This lectin is also an oligomeric protein composed of three subunits and mass spectrometry revealed similarities with a Sorghum bicolor protein. CasuL did not undergo unfolding when heated but changes in conformation and hemagglutinating activity were detected at basic pH. CasuL did not reduce the viability of human peripheral blood mononuclear cells but was toxic to leukemic K562 cells (IC5067.04±5.78μg/mL) and breast cancer T47D cells (IC50: 58.75±2.5μg/mL). CasuL (6.25-800μg/mL) only showed bacteriostatic effect but was able to reduce biofilm formation by Staphylococcus saprophyticcus and Staphylococcus aureus (non-resistant and oxacillin-resistant isolates). CasuL showed antifungal activity against Candida krusei causing alterations in cell morphology and damage to cell wall. In conclusion, the pinnulae of C. surinamensis leaves contain a thermo-stable lectin with biotechnological potential as cytotoxic, antibiofilm, and antifungal agent.
Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420 Recife, Brazil.
Thamara Figueiredo
TF
Procópio
Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420 Recife, Brazil.
Leydianne Leite
LL
de Siqueira Patriota
Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420 Recife, Brazil.
Maiara Celine
MC
de Moura
Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420 Recife, Brazil.
Pollyanna Michelle
PM
da Silva
Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420 Recife, Brazil.
Ana Patrícia Silva
AP
de Oliveira
Laboratório de Imunomodulação e Novas Abordagens Terapêuticas, Núcleo de Pesquisa em Inovação Terapêutica (NUPIT), Universidade Federal de Pernambuco, 50670-420 Recife, Pernambuco, Brazil.
Lidiane Vasconcelos
LV
do Nascimento Carvalho
Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420 Recife, Brazil.
Thâmarah
T
de Albuquerque Lima
Centro de Tecnologias Estratégicas do Nordeste, Recife, Pernambuco, Brazil.
Tatiana
T
Soares
Centro de Tecnologias Estratégicas do Nordeste, Recife, Pernambuco, Brazil.
Túlio Diego
TD
da Silva
Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420 Recife, Brazil.
Luana Cassandra
LC
Breitenbach Barroso Coelho
Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420 Recife, Brazil; Laboratório de Imunomodulação e Novas Abordagens Terapêuticas, Núcleo de Pesquisa em Inovação Terapêutica (NUPIT), Universidade Federal de Pernambuco, 50670-420 Recife, Pernambuco, Brazil.
Maira Galdino
MG
da Rocha Pitta
Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420 Recife, Brazil; Laboratório de Imunomodulação e Novas Abordagens Terapêuticas, Núcleo de Pesquisa em Inovação Terapêutica (NUPIT), Universidade Federal de Pernambuco, 50670-420 Recife, Pernambuco, Brazil.
Moacyr Jesus Barreto
MJ
de Melo Rêgo
Laboratório de Biologia Celular de Patógenos, Departamento de Microbiologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Cidade Universitária, 50670-420 Recife, Brazil.
Regina Celia
RC
Bressan Queiroz de Figueiredo
Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420 Recife, Brazil.
Patrícia Maria
PM
Guedes Paiva
Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420 Recife, Brazil. Electronic address: thiagohn86@yahoo.com.br.
Thiago Henrique
TH
Napoleão
D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D002175:Candida / Q000187:drug effects / Q000502:physiology; D045744:Cell Line, Tumor; D007887:Fabaceae / Q000737:chemistry*; D006801:Humans; D008826:Microbial Sensitivity Tests; D018515:Plant Leaves / Q000737:chemistry*; D037121:Plant Lectins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology
D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D037121:Plant Lectins
Antifungal activity; Biofilm; Cancer cells; Leguminous; Plant lectin