Polysaccharides as protectants for paper-based analytical devices with antibody.
Paper with immobilized protein can provide a low-cost platform for diagnostics, but evidence is emerging that the instability of protein on paper during shipping and storage severely limits its commercial development. Of these, paper-immobilized antibody, though widely used, is in urgent need of improvement in the shelf life. Herein we provided a detailed investigation of a simple and versatile approach for achieving stable antibody on paper to improve bioassay performance. Anti-Blood Group A IgM was chosen as the model antibody and its activity was tested via agglutination reactions. Representative polysaccharides (i.e., chitosan, sodium alginate and dextran) were used as protectants and their ability to stabilize antibody under heat and desiccation stresses was investigated. It was found that the presence of dextran with high concentration markedly stabilized paper-immobilized IgM for at least 120 days. Our results indicate that dextran with good film-forming ability could be a protectant for paper-immobilized antibody.
Department of Chemical Engineering, Monash University, Wellington Rd., Clayton, Victoria 3800, Australia.
Rong
R
Cao
Materials Science and Engineering, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Clayton, Victoria 3168, Australia.
Wendy
W
Tian
Department of Chemical Engineering, Monash University, Wellington Rd., Clayton, Victoria 3800, Australia. Electronic address: wei.shen@monash.edu.
Wei
W
Shen
D000371:Agglutination; D000464:Alginates / Q000737:chemistry*; D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D003890:Desiccation; D003911:Dextrans / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006358:Hot Temperature; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology*; D010209:Paper; D020011:Protective Agents / Q000737:chemistry*
D000464:Alginates; D000888:Antibodies, Anti-Idiotypic; D003911:Dextrans; D006603:Hexuronic Acids; D007075:Immunoglobulin M; D020011:Protective Agents; C044203:anti-IgM; D020723:Glucuronic Acid; D048271:Chitosan
Antibody immobilization; Dextran; Paper-based biosensor; Polysaccharide protection