Controlled surface morphology and hydrophilicity of polycaprolactone toward human retinal pigment epithelium cells.
Applying scaffolds as a bed to enhance cell proliferation and even differentiation is one of the treatment of retina diseases such as age-related macular degeneration (AMD) which deteriorating photoreceptors and finally happening blindness. In this study, aligned polycaprolactone (PCL) nanofibers were electrospun and at different conditions and their characteristics were measured by scanning electron microscope (SEM) and contact angle. Response surface methodology (RSM) was used to optimize the diameter of fabricated nanofibers. Two factors as solution concentration and voltage value were considered as independent variables and their effects on nanofibers' diameters were evaluated by central composite design and the optimum conditions were obtained as 0.12g/mL and 20kV, respectively. In order to decrease the hydrophobicity of PCL, the surface of the fabricated scaffolds was modified by alkaline hydrolysis method. Contact time of the scaffolds and alkaline solution and concentration of alkaline solution were optimized using Box Behnken design and (120min and 5M were the optimal, respectively). Contact angle measurement showed the high hydrophilicity of treated scaffolds (with contact angle 7.48°). Plasma surface treatment was applied to compare the effect of using two kinds of surface modification methods simultaneously on hydrolyzed scaffolds. The RPE cells grown on scaffolds were examined by immunocytochemistry (ICC), MTT and continuous inspection of cellular morphology. Interestingly, Human RPE cells revealed their characteristic morphology on hydrolyzed scaffold well. As a result, we introduced a culture substrate with low diameter (185.8nm), high porosity (82%) and suitable hydrophilicity (with contact angle 7.48 degree) which can be promising for hRPE cell transplantation.
Department of Life Science Engineering, Faculty of New sciences and Technologies, University of Tehran, Tehran, Iran.
Saleheh
S
Shahmoradi
Department of Life Science Engineering, Faculty of New sciences and Technologies, University of Tehran, Tehran, Iran.
Fatemeh
F
Yazdian
Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran. Electronic address: taban_f@nigeb.ac.ir.
Fatemeh
F
Tabandeh
Department of Molecular Medicine, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Zahra-Soheila
ZS
Soheili
Department of Life Science Engineering, Faculty of New sciences and Technologies, University of Tehran, Tehran, Iran.
Ashraf Sadat
AS
Hatamian Zarami
Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Mona
M
Navaei-Nigjeh
D001672:Biocompatible Materials / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002725:Chloroform / Q000737:chemistry; D006801:Humans; D006868:Hydrolysis; D057927:Hydrophobic and Hydrophilic Interactions; D007150:Immunohistochemistry; D007231:Infant, Newborn; D007633:Keratins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D057139:Nanofibers / Q000648:ultrastructure; D011091:Polyesters / Q000494:pharmacology*; D016062:Porosity; D055213:Retinal Pigment Epithelium / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry; D014867:Water / Q000737:chemistry
D001672:Biocompatible Materials; D011091:Polyesters; D014867:Water; C016240:polycaprolactone; D007633:Keratins; D002725:Chloroform
Age-related macular degeneration; Alkaline hydrolysis; Polycaprolactone; Retina; Retinal pigment epithelium