Combining molecular and bioprocess techniques to produce poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) with controlled monomer composition by Burkholderia sacchari.
Biopolymers as polyhydroxyalkanoates (PHA) composed by different co-monomers 3-hydroxybutyrate and 3-hydroxyhexanoate [P(3HB-co-3HHx)] has attracted interest since its properties are similar to low density polyethylene. Burkholderia sacchari produces this copolymer with a very low 3HHx molar fraction, about 2 mol%. B. sacchari mutant (unable to produce polymer) was engineered to host PHA biosynthesis genes (phaPCJ) from Aeromonas sp. In addition, a two-step bioprocess to increase biopolymer production was developed. The combination of these techniques resulted in the production of P(3HB-co-3HHx) with 3HHx content up to 20 mol%. The PHA content was about 78% of dry biomass, resulting in PHA volumetric productivities around 0.45gl-1h-1. The P(3HB-co-3HHx) containing 20 mol% of 3HHx presented an elongation at brake of 945%, higher than reported before for this PHA composition. Here we have described an approach to increase 3HHx content into the copolymer, allowing the precise control of the 3HHx molar fractions.
Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brazil. Electronic address: thatianemendonca@usp.br.
Thatiane T
TT
Mendonça
Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brazil.
Rafaela R
RR
Tavares
Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brazil.
Lucas G
LG
Cespedes
Centro de Ciências e Tecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, Brazil.
Ruben J
RJ
Sánchez-Rodriguez
Centro de Ciências e Tecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, Brazil.
Jan
J
Schripsema
Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brazil.
Marilda K
MK
Taciro
Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brazil.
José G C
JG
Gomez
Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brazil. Electronic address: lukneif@usp.br.
Luiziana F
LF
Silva
D020155:3-Hydroxybutyric Acid / Q000096:biosynthesis* / Q000737:chemistry; D000333:Aeromonas / Q000737:chemistry; D001704:Biopolymers / Q000096:biosynthesis* / Q000737:chemistry; D019117:Burkholderia / Q000737:chemistry*; D002208:Caproates / Q000737:chemistry; D005285:Fermentation; D054813:Polyhydroxyalkanoates / Q000737:chemistry; D000094122:Polyhydroxybutyrates
D020155:3-Hydroxybutyric Acid; D001704:Biopolymers; D002208:Caproates; D054813:Polyhydroxyalkanoates; C115940:poly(3-hydroxybutyrate-co-3-hydroxyhexanoate); D000094122:Polyhydroxybutyrates
Aeromonas sp.; Burkholderia sacchari; Modulated PHA composition; Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate); pha PCJ operon