Utilization of carbon sources by clinical isolates of Aeromonas.
Bacteria in the genus Aeromonas are primarily aquatic organisms; however, some species can cause diseases in humans, ranging from wound infections to septicemia, of which diarrhea is the most common condition. The ability to use a variety of carbon substrates is advantageous for pathogenic bacteria. Therefore, we used Biolog GN2 microplates to analyze the ability of 103 clinical, predominantly diarrheal, isolates of Aeromonas to use various carbon sources, and we verified whether, among the substrates metabolized by these strains, there were some endogenous to the human intestine. The results indicate that Aeromonas present great diversity in the utilization of carbon sources, and that they preferentially use carbohydrates and amino acids as carbon sources. Among the carbon sources metabolized by Aeromonas in vitro, some were found to be components of intestinal mucin, including aspartic acid, glutamic acid, l-serine, galactose, N-acetyl-glucosamine, and glucose, which were used by all strains tested. Additionally, mannose, d-serine, proline, threonine, and N-acetyl-galactosamine were used by several strains. The potential to metabolize substrates endogenous to the intestine may contribute to Aeromonas' capacity to grow in and colonize the intestine. We speculate that this may help explain the ability of Aeromonas to cause diarrhea.
a Departamento de Análises Clínicas, Universidade Federal do Paraná, Curitiba-PR, Brazil.
Karoline C
KC
Prediger
a Departamento de Análises Clínicas, Universidade Federal do Paraná, Curitiba-PR, Brazil.
Monica
M
Surek
a Departamento de Análises Clínicas, Universidade Federal do Paraná, Curitiba-PR, Brazil.
Cibelle B
CB
Dallagassa
a Departamento de Análises Clínicas, Universidade Federal do Paraná, Curitiba-PR, Brazil.
Flávia E A
FE
Assis
b Departamento de Farmácia, Universidade Federal do Paraná, Curitiba-PR, Brazil.
Mario S
MS
Piantavini
c Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba-PR, Brazil.
Emanuel M
EM
Souza
c Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba-PR, Brazil.
Fábio O
FO
Pedrosa
d Laboratório Central do Estado do Paraná (LACEN-PR), Curitiba-PR, Brazil.
Sônia M S S
SM
Farah
a Departamento de Análises Clínicas, Universidade Federal do Paraná, Curitiba-PR, Brazil.
Dayane
D
Alberton
a Departamento de Análises Clínicas, Universidade Federal do Paraná, Curitiba-PR, Brazil.
Cyntia M T
CM
Fadel-Picheth
D000333:Aeromonas / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002241:Carbohydrates; D002244:Carbon / Q000378:metabolism*; D003967:Diarrhea / Q000209:etiology; D006801:Humans; D007422:Intestines / Q000382:microbiology
D002241:Carbohydrates; D002244:Carbon
Aeromonas; Biolog; carbon source; mucin; mucine; source de carbone