Decolourisation Capabilities of Ligninolytic Enzymes Produced byMarasmius cladophyllusUMAS MS8 on Remazol Brilliant Blue R and Other Azo Dyes.
Marasmius cladophylluswas examined for its ability to degradatively decolourise the recalcitrant dye Remazol Brilliant Blue R (RBBR) and screened for the production of ligninolytic enzymes using specific substrates. Monitoring dye decolourisation by the decrease in absorbance ratio ofA592/A500shows that the decolourisation of RBBR dye was associated with the dye degradation.Marasmius cladophyllusproduces laccase and lignin peroxidase in glucose minimal liquid medium containing RBBR. Both enzyme activities were increased, with laccase activity recorded 70 times higher reaching up to 390 U L-1on day 12. Further in vitro RBBR dye decolourisation using the culture medium shows that laccase activity was correlated with the dye decolourisation. Fresh RBBR dye continuously supplemented into the decolourised culture medium was further decolourised much faster in the subsequent round of the RBBR dye decolourisation. In vitro dye decolourisation using the crude laccase not only decolourised 76% of RBBR dye in just 19 hours but also decolourised 54% of Orange G and 33% of Congo red at the same period of time without the use of any exogenous mediator. This rapid dye decolourisation ability of the enzymes produced byM. cladophyllusthus suggested its possible application in the bioremediation of dye containing wastewater.
Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia.
Ngieng Ngui
0000-0002-8703-1509
NN
Sing
Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia.
Ahmad
0000-0002-6559-5602
A
Husaini
Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia.
Azham
0000-0001-9173-8171
A
Zulkharnain
Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia.
Hairul Azman
HA
Roslan
D000645:Ammonium Sulfate / Q000737:chemistry; D000880:Anthraquinones / Q000378:metabolism*; D001391:Azo Compounds / Q000378:metabolism*; D011232:Chemical Precipitation; D003116:Color; D004396:Coloring Agents / Q000378:metabolism*; D003224:Congo Red; D007527:Isoenzymes / Q000378:metabolism; D042845:Laccase / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism; D055433:Marasmius / Q000201:enzymology*; D010544:Peroxidases / Q000378:metabolism*
D000880:Anthraquinones; D001391:Azo Compounds; D004396:Coloring Agents; D007527:Isoenzymes; C008710:Orange G; D003224:Congo Red; D008031:Lignin; D042845:Laccase; D010544:Peroxidases; C042858:lignin peroxidase; C024758:Remazol Brilliant Blue R; D000645:Ammonium Sulfate