Gas-liquid interfacial plasmas producing reactive species for cell membrane permeabilization.
Gas-liquid interfacial atmospheric-pressure plasma jets (GLI-APPJ) are used medically for plasma-induced cell-membrane permeabilization. In an attempt to identify the dominant factors induced by GLI-APPJ responsible for enhancing cell-membrane permeability, the concentration and distribution of plasma-produced reactive species in the gas and liquid phase regions are measured. These reactive species are classified in terms of their life-span: long-lived (e.g., H2O2), short-lived (e.g., O2•-), and extremely-short-lived (e.g.,•OH). The concentration of plasma-produced•OHaqin the liquid phase region decreases with an increase in solution thickness (<1 mm), and plasma-induced cell-membrane permeabilization is found to decay markedly as the thickness of the solution increases. Furthermore, the horizontally center-localized distribution of•OHaq, resulting from the center-peaked distribution of•OH in the gas phase region, corresponds with the distribution of the permeabilized cells upon APPJ irradiation, whereas the overall plasma-produced oxidizing species such as H2O2aqin solution exhibit a doughnut-shaped horizontal distribution. These results suggest that•OHaqis likely one of the dominant factors responsible for plasma-induced cell-membrane permeabilization.
Department of Electronic Engineering, Tohoku University, 6-6-05 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan.
Toshiro
T
Kaneko
Department of Electronic Engineering, Tohoku University, 6-6-05 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan.
Shota
S
Sasaki
Department of Electronic Engineering, Tohoku University, 6-6-05 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan.
Keisuke
K
Takashima
Department of Biomedical Engineering, Tohoku University, 6-6-05 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan.
Makoto
M
Kanzaki
atmospheric-pressure plasma jet; cell membrane permeabilization; gas-liquid interfacial plasma; plasma medicine; reactive species