Preparation ofArjunāriṣṭaUsing Microbes Isolated fromWoodfordia fruticosaFlowers (Dhayati).
OBJECTIVE
To verify the utility of isolated fermentative microbes fromWoodfordia fruticosaflowers for preparation of Arjunāriṣṭa formulation and its comparative evaluation with the same formulation prepared by traditional method.


METHODS
In the present technique, isolated colonies of microorganisms fromWoodfordia fruticosa(Dhātakī) flowers on Saubroad dextrose media were separated and suspended in sterile water. This suspension was aseptically added in previously sterilized mixtures containing all intended ingredients for Arjunāriṣṭa which was thereafter incubated for 20 days at 37°C to achieve optimal fermentation. The formulation thus obtained was further subjected to various evaluation tests.


RESULTS
Arjunāriṣṭa was prepared using a new approach, and in that, isolated microorganisms from the flowers ofWoodfordia fruticosa(Dhātakī) were used. It was found that the new approach was successful in generating approximately same quantities of alcohol content in comparison with traditional methods which have shown varying concentration of alcoholic content. Moreover, the new process prevents the growth of unwanted microbes thus, optimizing standards for purity and safety of the formulation.


CONCLUSIONS
The formulation prepared by a new procedure showed marked uniformity for different parameters such as alcohol production, total phenol content, total solid content as compared to that prepared by the traditional method. Similarly, the results of thin layer chromatography, high performance thin layer chromatography showed marked uniformity related to quality, safety, efficacy, and reproducibility of the new method as compared to the traditional one. Thus, the modern technique was found to show reproducibility and facilitate easier quality assessment.
Department of Pharmaceutics, Rajarambapu College of Pharmacy, Sangli, Maharashtra, India.
Dheeraj S
DS
Randive
Department of Pharmaceutics, Amrutvahini College of Pharmacy, Ahmednagar, Maharashtra, India.
Sadik F
SF
Sayyad
Department of Pharmaceutical Chemistry, Rajarambapu College of Pharmacy, Sangli, Maharashtra, India.
Somnath Devidas
SD
Bhinge
Department of Pharmaceutical Chemistry, Rajarambapu College of Pharmacy, Sangli, Maharashtra, India.
Mangesh A
MA
Bhutkar
Arjunariṣṭa formulation; Ayurveda; HPTLC; TLC; modern technique