Secondary Ion Mass Spectrometry Analysis of Renal Cell Carcinoma with Electrospray Droplet Ion Beams.
Tissue samples from renal cell carcinoma patients were analyzed by electrospray droplet ion beam-induced secondary ion mass spectrometry (EDI/SIMS). Positively- and negatively-charged secondary ions were measured for the cancerous and noncancerous regions of the tissue samples. Although specific cancerous species could not be found in both the positive and negative secondary ion spectra, the spectra of the cancerous and noncancerous tissues presented different trends. For instance, in them/zrange of 500-800 of the positive secondary ion spectra for the cancerous tissues, the intensities for severalm/zvalues were lower than those of them/z+2 peaks (indicating one double bond loss for the species), whereas, for the noncancerous tissues, the inverse trend was obtained. The tandem mass spectrometry (MS/MS) was also performed on the tissue samples using probe electrospray ionization (PESI), and some molecular ions produced by PESI were found to be fragmented into the ions observed in EDI/SIMS analysis. When the positive secondary ion spectra produced by EDI/SIMS were analyzed by principal component analysis, the results for cancerous and noncancerous tissues were separated. The EDI/SIMS method can be applied to distinguish between a cancerous and a noncancerous area with high probability.
Interdisciplinary Graduate School, University of Yamanashi.
Satoshi
S
Ninomiya
Department of Anatomy and Cell Biology, Faculty of Medicine, University of Yamanashi.
Kentaro
K
Yoshimura
Interdisciplinary Graduate School, University of Yamanashi.
Lee Chuin
LC
Chen
Department of Anatomy and Cell Biology, Faculty of Medicine, University of Yamanashi.
Sen
S
Takeda
Department of Anatomy and Cell Biology, Faculty of Medicine, University of Yamanashi.
Kenzo
K
Hiraoka
SIMS; electrospray droplet; renal cell carcinoma