Dominating types of penicillinase-plasmids in Neisseria gonorrhoeae strains isolated in 2010-2012 in Warsaw.
BACKGROUND
The reason of Neisseria gonorrhoeae resistance to penicillin is often production of TEM beta-lactamases encoded by plasmids. The most common types of the plasmid are Africa, Asia and Toronto/Rio. Another reason of resistance can be mutations in bacterial chromosome. The aim of the study was to investigate the types of plasmids occurring in in Neisseria gonorrhoeae strains isolated in 2010-2012 in Warsaw.


METHODS
From 218 isolated in 2010, 2011 and at the beginning of 2012 from patients of Medical University in Warsaw we selected 12 strains producing beta- lactamase (penicillinase producing N. gonorrhoeae, PPNG). d B-tests to investigate bacterial sensitivity to penicillin and cefiriaxon. The types of plasmids were determined with PCR.


RESULTS
The Beta-lactamases were encoded by Toronto/Rio (41,7%), Asia (33,3%) and Africa (25,0%) plasmids. All the strains were resistant to penicillin (MIC 2-8 mg/L) and sensitive to ceftriaxon (MIC 0,004-0,032 mg/L).


CONCLUSIONS
All of the investigate PPNG strains were penicillin resistant and ceftriaxon sensitive. The dominating type of the penicillinase plasmid was Toronto/Rio.
Beata
B
Mlynarczyk-Bonikowska
Marlena
M
Kujawa
Graiyna
G
Mynarczyk
Magdalena
M
Malejczyk
Slawomir
S
Majewski
D001426:Bacterial Proteins / Q000235:genetics*; D006069:Gonorrhea / Q000175:diagnosis / Q000201:enzymology / Q000235:genetics; D006801:Humans; D009344:Neisseria gonorrhoeae / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D010403:Penicillin Resistance / Q000235:genetics*; D010405:Penicillinase / Q000235:genetics*; D010957:Plasmids; D011044:Poland
D001426:Bacterial Proteins; D010405:Penicillinase