Preparation of a poly(styrene-co-DPHA-co-EDMA) monolith and its application for the separation of small molecules and biomacromolecules by HPLC.
A high performance liquid chromatography (HPLC) monolithic column was prepared by redox polymerization of styrene, dipentaerythritol hexaacrylate (DPHA) and ethylene glycol dimethacrylate (EDMA) in a porogen system of n-propanol/PEG400. The monolith was characterized by scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP) and the results indicated that the monolith had a stable and homogeneous structure. The porosity of the monolithic column was 75.86% and average pore diameter was 2.1µm. Several alkylbenzenes and anilines were used to evaluate the column performance in terms of hydrophobicity. Then the column was applied to separate small molecules including phytosterol and BSA tryptic digest. Finally, five standard proteins, egg white and plasma were separated respectively and high separation capacity of protein was obtained.
College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Baoding 071002, China.
Xixi
X
Wang
College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Baoding 071002, China.
Xueying
X
Li
College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Baoding 071002, China.
Xiaoya
X
Jiang
College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Baoding 071002, China.
Peipei
P
Dong
College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Baoding 071002, China. Electronic address: lhy1610@126.com.
Haiyan
H
Liu
College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Baoding 071002, China.
Ligai
L
Bai
College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Baoding 071002, China.
Hongyuan
H
Yan
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008689:Methacrylates / Q000737:chemistry*; D010446:Peptide Fragments / Q000032:analysis / Q000302:isolation & purification*; D010840:Phytosterols / Q000032:analysis / Q000302:isolation & purification*; D011092:Polyethylene Glycols / Q000737:chemistry*; D016062:Porosity; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D020058:Styrene / Q000737:chemistry*
D008689:Methacrylates; D010446:Peptide Fragments; D010840:Phytosterols; D011506:Proteins; C421283:poly(ethylene glycol)-dimethacrylate; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D020058:Styrene; C004919:ethylene dimethacrylate; C000595213:polyethylene glycol 400
Monolithic column; Phytosterol; Preparation and application; Protein; Small molecules