Proof of pore formation and biophysical perturbations through a 2D amoxicillin-lipid membrane interaction approach.
Amoxicillin is a worldwide used antibiotic, and it is classified as a first-line drug against Helicobacter pylori gastric infections. However, the current treatment of these infections has several limitations, such as the side effects and the low therapeutic compliance. Amoxicillin has been associated with gastrointestinal and renal side effects, with higher toxicity when the pH is lower. By considering this association and the well-known pH gradient of the gastric mucosa, this work aims to evaluate the influence of pH on the toxicity of amoxicillin. For that purpose, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) monolayers were used since phosphatidylcholines are the most common phospholipid headgroup of biological membranes. To have insight of the effects of amoxicillin, different techniques were employed, namely, isotherm measurements, infrared reflection-absorption spectroscopy, grazing incident X-ray diffraction and Brewster angle microscopy. The monolayers of DPPC spread onto different buffer solutions (pH1.2, pH5 and pH7.4) showed different structural and packing properties. The interaction with amoxicillin also depended on the pH. At pH7.4, the highest effect was visualized at lower pressures, with partial restoration of the biophysical properties of the monolayer at 30 mN.m-1. A higher perturbation is shown at acidic pH, in which pores were visualized by Brewster angle microscopy. These perturbations may ultimately be related with amoxicillin toxicity.
UCIBIO, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Portugal.
Daniela
D
Lopes
UCIBIO, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Portugal.
Cláudia
C
Nunes
Synchrotron SOLEIL, L'Orme des Merisiers, Saint Aubin, BP48, 91192, Gif-sur-Yvette, France.
Philippe
P
Fontaine
I3S, Instituto de Investigação e Inovação em Saúde and INEB - Instituto de Engenharia Biomédica, Portugal; CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Portugal.
Bruno
B
Sarmento
UCIBIO, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Portugal. Electronic address: shreis@ff.up.pt.
Salette
S
Reis
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D000658:Amoxicillin / Q000633:toxicity*; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D001703:Biophysics; D006863:Hydrogen-Ion Concentration; D008051:Lipid Bilayers / Q000737:chemistry*; D008853:Microscopy; D014961:X-Ray Diffraction
D000900:Anti-Bacterial Agents; D008051:Lipid Bilayers; D015060:1,2-Dipalmitoylphosphatidylcholine; D000658:Amoxicillin
amoxicillin; biophysics; monolayers; pores