Multimodal optical analysis discriminates freshly extracted human sample of gliomas, metastases and meningiomas from their appropriate controls.
Delineating tumor margins as accurately as possible is of primordial importance in surgical oncology: extent of resection is associated with survival but respect of healthy surrounding tissue is necessary for preserved quality of life. The real-time analysis of the endogeneous fluorescence signal of brain tissues is a promising tool for defining margins of brain tumors. The present study aims to demonstrate the feasibility of multimodal optical analysis to discriminate fresh samples of gliomas, metastases and meningiomas from their appropriate controls. Tumor samples were studied on an optical fibered endoscope using spectral and fluorescence lifetime analysis and then on a multimodal set-up for acquiring spectral, one and two-photon fluorescence images, second harmonic generation signals and two-photon fluorescence lifetime datasets. The obtained data allowed us to differentiate healthy samples from tumor samples. These results confirmed the possible clinical relevance of this real-time multimodal optical analysis. This technique can be easily applied to neurosurgical procedures for a better delineation of surgical margins.
IMNC Laboratory, UMR 8165-CNRS/IN2P3, Paris-Saclay university, 91405 Orsay, France.|Neurosurgery Department, Sainte-Anne Hospital, France.|Paris Descartes University, Paris, France.
Marc
M
Zanello
IMNC Laboratory, UMR 8165-CNRS/IN2P3, Paris-Saclay university, 91405 Orsay, France.
Fanny
F
Poulon
Neurosurgery Department, Sainte-Anne Hospital, France.|Paris Descartes University, Paris, France.
Johan
J
Pallud
Paris Descartes University, Paris, France.|Neuropathology Department, Sainte-Anne Hospital, France.
Pascale
P
Varlet
Center of Advanced European Studies and Research (caesar), 53175 Bonn, Germany.
H
H
Hamzeh
Neurosurgery Department, Sainte-Anne Hospital, France.|Paris Descartes University, Paris, France.
Georges
G
Abi Lahoud
Paris Descartes University, Paris, France.|Neuropathology Department, Sainte-Anne Hospital, France.
Felipe
F
Andreiuolo
IMNC Laboratory, UMR 8165-CNRS/IN2P3, Paris-Saclay university, 91405 Orsay, France.
Ali
A
Ibrahim
Paris Descartes University, Paris, France.|Neuropathology Department, Sainte-Anne Hospital, France.
Mélanie
M
Pages
Paris Descartes University, Paris, France.|Neuropathology Department, Sainte-Anne Hospital, France.
Fabrice
F
Chretien
Neurosurgery Department, Lyon Hospital, France.
Federico
F
Di Rocco
Neurosurgery Department, Sainte-Anne Hospital, France.|Paris Descartes University, Paris, France.
Edouard
E
Dezamis
Neurosurgery Department, Sainte-Anne Hospital, France.|Paris Descartes University, Paris, France.
François
F
Nataf
Neurosurgery Department, Sainte-Anne Hospital, France.|Paris Descartes University, Paris, France.
Baris
B
Turak
Neurosurgery Department, Sainte-Anne Hospital, France.|Paris Descartes University, Paris, France.
Bertrand
B
Devaux
IMNC Laboratory, UMR 8165-CNRS/IN2P3, Paris-Saclay university, 91405 Orsay, France.|Paris Diderot University, Sorbonne Paris Cité, F-75013, Paris, France.
Darine
D
Abi Haidar
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D003131:Combined Modality Therapy; D005260:Female; D005910:Glioma / Q000000981:diagnostic imaging* / Q000473:pathology* / Q000628:therapy; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D008579:Meningioma / Q000000981:diagnostic imaging* / Q000473:pathology* / Q000628:therapy; D008853:Microscopy / Q000379:methods; D008875:Middle Aged; D064847:Multimodal Imaging / Q000379:methods; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D061848:Optical Imaging / Q000379:methods