Removing bias against short sequences enables northern blotting to better complement RNA-seq for the study of small RNAs.
Changes in small non-coding RNAs such as micro RNAs (miRNAs) can serve as indicators of disease and can be measured using next-generation sequencing of RNA (RNA-seq). Here, we highlight the need for approaches that complement RNA-seq, discover that northern blotting of small RNAs is biased against short sequences and develop a protocol that removes this bias. We found that multiple small RNA-seq datasets from the worm Caenorhabditis elegans had shorter forms of miRNAs that appear to be degradation products that arose during the preparatory steps required for RNA-seq. When using northern blotting during these studies, we discovered that miRNA-length probes can have ∼1000-fold bias against detecting even synthetic sequences that are 8 nt shorter. By using shorter probes and by performing hybridization and washes at low temperatures, we greatly reduced this bias to enable nearly equivalent detection of 24 to 14 nt RNAs. Our protocol can discriminate RNAs that differ by a single nucleotide and can detect specific miRNAs present in total RNA from C. elegans and pRNAs in total RNA from bacteria. This improved northern blotting is particularly useful to analyze products of RNA processing or turnover, and functional RNAs that are shorter than typical miRNAs.
Department of Cell Biology and Molecular Genetics, University of Maryland, College, Park, MD 20742, USA.
Yun S
YS
Choi
Department of Cell Biology and Molecular Genetics, University of Maryland, College, Park, MD 20742, USA.
Lanelle O
LO
Edwards
Department of Cell Biology and Molecular Genetics, University of Maryland, College, Park, MD 20742, USA.
Aubrey
A
DiBello
Department of Cell Biology and Molecular Genetics, University of Maryland, College, Park, MD 20742, USA.
Antony M
AM
Jose
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern / Q000379:methods*; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism; D015342:DNA Probes / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics* / Q000378:metabolism; D018510:RNA, Helminth / Q000235:genetics* / Q000378:metabolism; D012680:Sensitivity and Specificity; D017423:Sequence Analysis, RNA
D015342:DNA Probes; D035683:MicroRNAs; D012329:RNA, Bacterial; D018510:RNA, Helminth