Risks of using EDTA as an agent for trace metals dosing in anaerobic digestion of olive mill solid waste.
Low concentrations of trace elements in many organic wastes recommend their supplementation in order to avoid potential limitations. Different chelating agents have been used to ensure an adequate trace metal pool in the soluble fraction, by forming dissolved complexes. Ethylenediaminetetraacetic acid (EDTA) is probably the most common, although several negative effects could be associated with its usage. Biomethane potential tests were performed using Olive Mill Solid Waste as the substrate, supplementing different combinations of Fe, Co, Ni, Ba, always under the presence of EDTA. Results show that Ni and Co slightly recovered biodegradability. However, Ba supplementation resulted in worsening the methane yield coefficient in all cases. High concentration of EDTA led to decrease in the activity of anaerobic digestion. High availability of EDTA induces the capture of trace metals like Co or Ni, key trace metals for anaerobic biomass activity. While supplementing trace metals, the addition of Ba and/or EDTA must be carefully considered.
a Instituto de la Grasa (C.S.I.C.), Edificio 46 , Campus Universitario Pablo de Olavide , Sevilla , Spain.
A
http://orcid.org/0000-0002-4615-5038
A
Serrano
b Master of Engineering Sciences with Specialization in Biotechnology , Universidad de La Frontera , Temuco , Chile.|c Escuela de Procesos Industriales, Facultad de Ingeniería , Universidad Católica de Temuco , Temuco , Chile.
F
http://orcid.org/0000-0001-6005-886X
F
Pinto-Ibieta
d Biological Processes Laboratory, Center for Research, Development and Innovation in Environmental Engineering, São Carlos School of Engineering (EESC) , University of São Paulo (USP) , São Carlos , Brazil.
A F M
http://orcid.org/0000-0003-3464-2469
AFM
Braga
e Department of Chemical Engineering , Universidad de La Frontera , Temuco , Chile.
D
http://orcid.org/0000-0002-2234-8257
D
Jeison
a Instituto de la Grasa (C.S.I.C.), Edificio 46 , Campus Universitario Pablo de Olavide , Sevilla , Spain.
R
http://orcid.org/0000-0002-3699-7223
R
Borja
a Instituto de la Grasa (C.S.I.C.), Edificio 46 , Campus Universitario Pablo de Olavide , Sevilla , Spain.
F G
http://orcid.org/0000-0002-2586-007X
FG
Fermoso
D000693:Anaerobiosis; D019149:Bioreactors; D002614:Chelating Agents / Q000737:chemistry*; D004492:Edetic Acid / Q000737:chemistry*; D005525:Food-Processing Industry; D007220:Industrial Waste / Q000032:analysis*; D031658:Olea / Q000737:chemistry; D012306:Risk; D062611:Solid Waste / Q000032:analysis*; D014131:Trace Elements / Q000737:chemistry*
D002614:Chelating Agents; D007220:Industrial Waste; D062611:Solid Waste; D014131:Trace Elements; D004492:Edetic Acid
Ethylenediaminetetraacetic acid; metal affinity; methane production rate; microbial activity; olive mill solid waste; trace metal supplementation