Insights using the molecular model of Lipoxygenase from Finger millet (Eleusine coracana (L.)).
Lipoxygenase-1 (LOX-1) protein provides defense against pests and pathogens and its presence have been positively correlated with plant resistance against pathogens. Linoleate is a known substrate of lipoxygenase and it induces necrosis leading to the accumulation of isoflavonoid phytoalexins in plant leaves. Therefore, it is of interest to study the structural features of LOX-1 from Finger millet. However, the structure ofLOX-1 from Finger millet is not yet known. A homology model of LOX-1 from Finger millet is described. Domain architecture study suggested the presence of two domains namely PLAT (Phospho Lipid Acyl Transferase) and lipoxygenase. Molecular docking models of linoleate with lipoxygenase from finger millet, rice and sorghum are reported. The features of docked models showed that finger millet have higher pathogen resistance in comparison to other cereal crops. This data is useful for the molecular cloning of fulllength LOX-1 gene for validating its role in improving plant defense against pathogen infection and for various other biological processes.
Department of Molecular Biology & Genetic Engineering, CBSH, G. B. Pant University of Agriculture & Technology, Pantnagar-263145,Uttarakhand, Bharat (India).|Department of Computational Biology & Bioinformatics, JSBB, Sam Higginbottom Institute of Agriculture,Technology and Sciences, Allahabad-211007, Uttar Pradesh, Bharat (India).
Apoorv
A
Tiwari
Department of Molecular Biology & Genetic Engineering, CBSH, G. B. Pant University of Agriculture & Technology, Pantnagar-263145,Uttarakhand, Bharat (India).|Department of Computational Biology & Bioinformatics, JSBB, Sam Higginbottom Institute of Agriculture,Technology and Sciences, Allahabad-211007, Uttar Pradesh, Bharat (India).
Himanshu
H
Avashthi
Uttaranchal Institute of Technology, Uttaranchal University, Arcadia Grant, Dehradun-248007, Uttarakhand Bharat (India).
Richa
R
Jha
Department of Biotechnology, Indian Institute of Technology Madras, Chennai-600036, Tamil Nadu, Bharat (India).
Ambuj
A
Srivastava
Department of Molecular Biology & Genetic Engineering, CBSH, G. B. Pant University of Agriculture & Technology, Pantnagar-263145,Uttarakhand, Bharat (India).|Department of Computational Biology & Bioinformatics, JSBB, Sam Higginbottom Institute of Agriculture,Technology and Sciences, Allahabad-211007, Uttar Pradesh, Bharat (India).
Vijay
V
Kumar Garg
Department of Biological Science, School of Basic Science, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad-211007, Uttar Pradesh, Bharat (India).
Pramod
P
Wasudev Ramteke
Department of Molecular Biology & Genetic Engineering, CBSH, G. B. Pant University of Agriculture & Technology, Pantnagar-263145,Uttarakhand, Bharat (India).
Anil
A
Kumar
Eleusine coracana; Lipoxygenase; Structure prediction; docking; homology modeling; pest resistance; protein-protein interaction