Antimicrobial and anticancer activities of porous chitosan-alginate biosynthesized silver nanoparticles.
The main aim of this study was to obtain porous antimicrobial composites consisting of chitosan, alginate, and biosynthesized silver nanoparticles (AgNPs). Chitosan and alginate were used owing to their pore-forming capacity, while AgNPs were used for their antimicrobial property. The developed porous composites of chitosan-alginate-AgNPs were characterized using Fourier transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy, X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM). The FT-IR results revealed the presence of a strong chemical interaction between chitosan and alginate due to polyelectrolyte complex; whereas, the XRD results confirmed the presence of AgNPs in the composites. The dispersion of AgNPs in the porous membrane was uniform with a pore size of 50-500μm. Antimicrobial activity of the composites was checked with Escherichia coli and Staphylococcus aureus. The developed composites resulted in the formation of a zone of inhibition of 11±1mm for the Escherichia coli, and 10±1mm for Staphylococcus aureus. The bacterial filtration efficiency of chitosan-alginate-AgNPs was 1.5-times higher than that of the chitosan-alginate composite. The breast cancer cell line MDA-MB-231 was used to test the anticancer activity of the composites. The IC50value of chitosan-alginate-AgNPs on MDA-MB-231 was 4.6mg. The developed chitosan-alginate-AgNPs composite showed a huge potential for its applications in antimicrobial filtration and cancer treatment.
Division of Bioengineering, Incheon National University, Incheon 406-772, Republic of Korea.
Jayachandran
J
Venkatesan
Institute for Life Science of Seogo (ILSS), Kolmar Korea Co, Seoul 137-876, Republic of Korea.
Jin-Young
JY
Lee
Institute for Life Science of Seogo (ILSS), Kolmar Korea Co, Seoul 137-876, Republic of Korea.
Dong Seop
DS
Kang
Department of Preventive Dental Sciences, College of Dentistry, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia.
Sukumaran
S
Anil
Institute for Life Science of Seogo (ILSS), Kolmar Korea Co, Seoul 137-876, Republic of Korea.
Se-Kwon
SK
Kim
Division of Bioengineering, Incheon National University, Incheon 406-772, Republic of Korea. Electronic address: msshim@inu.ac.kr.
Min Suk
MS
Shim
Specialized Graduate School Science & Technology Convergence, Pukyong National University, Busan 608-739, Republic of Korea. Electronic address: dgkim@pknu.ac.kr.
Dong Gyu
DG
Kim
D000464:Alginates / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D016062:Porosity; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects
D000464:Alginates; D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D006603:Hexuronic Acids; D012834:Silver; D020723:Glucuronic Acid; D048271:Chitosan
Alginate; Antimicrobial; Chitosan; Composites; Silver nanoparticles