Dexamethasone treatment decreases replication of viral hemorrhagic septicemia virus in Epithelioma papulosum cyprini cells.
The expression of Mx1 in EPC cells after treatment with poly(I:C) or infection with viral hemorrhagic septicemia virus (VHSV) was significantly suppressed by treatment with dexamethasone. However, the titer of VHSV did not increase but instead decreased after dexamethasone treatment. This suggests that dexamethasone not only downregulates type I IFN but also affects certain factors that are necessary for VHSV replication. An important effect of HSP90 on replication of RNA viruses and downregulation of HSP90 by glucocorticoids have been reported. In this study, dexamethasone downregulated HSP90α expression in EPC cells that were stimulated with poly(I:C) or infected with VHSV. Furthermore, cells treated with an HSP90 inhibitor, geldanamycin, showed significantly decreased titers of VHSV, suggesting that HSP90 may be an important host component involved in VHSV replication, and HSP90 inhibition might be one of the causes for the observed reduction in viral titer caused by dexamethasone treatment.
Graduate School of Integrated Bioindustry, Sejong University, Seoul, 05006, South Korea.
Min Sun
MS
Kim
Incheon Airport Regional Office, National Fishery Products Quality Management Service, Incheon, 22382, South Korea.
Su Jin
SJ
Lee
Department of Aquatic Life Medicine, Pukyong National University, Busan, 48513, South Korea.
Seung Hyuk
SH
Choi
Department of Aquatic Life and Medical Sciences, Sun Moon University, Asan, 31460, Chungnam, South Korea.
Yue Jai
YJ
Kang
Department of Aquatic Life Medicine, Pukyong National University, Busan, 48513, South Korea. khkim@pknu.ac.kr.
Ki Hong
KH
Kim
D000818:Animals; D016227:Benzoquinones / Q000494:pharmacology; D002277:Carcinoma / Q000378:metabolism / Q000821:virology; D045744:Cell Line, Tumor; D003530:Cyprinidae / Q000821:virology*; D004261:DNA Replication / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology*; D015536:Down-Regulation; D005393:Fish Diseases / Q000821:virology*; D018841:HSP90 Heat-Shock Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D007165:Immunosuppression Therapy; D007370:Interferon Type I / Q000096:biosynthesis; D047029:Lactams, Macrocyclic / Q000494:pharmacology; D064547:Myxovirus Resistance Proteins / Q000096:biosynthesis*; D029141:Novirhabdovirus / Q000235:genetics* / Q000276:immunology; D011070:Poly I-C / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects
D016227:Benzoquinones; D018841:HSP90 Heat-Shock Proteins; D007370:Interferon Type I; D047029:Lactams, Macrocyclic; D064547:Myxovirus Resistance Proteins; D003907:Dexamethasone; D011070:Poly I-C; C001277:geldanamycin
Dexamethasone; HSP90; Replication; Type I IFN; VHSV