The association between male-biased sex ratio and indicators of stress in red-spotted newts.
In populations with a male-biased operational sex ratio, coercive mating by males can have fitness consequences for females. One component of reduced fitness for females in populations with a male-biased OSR may be greater activation of the stress response, resulting in higher corticosterone release rates (CORT; a glucocorticoid stress hormone in amphibians). We test the hypothesis that a male-biased sex ratio affects female activity and release rates of CORT and testosterone (T) in male and female red-spotted newts (Notophthalmus viridescens). First, we evaluated if chemical cues from a male-biased sex ratio affect activity and CORT release rates in females. We predicted that females exposed to chemical cues of three males would be less active and have higher CORT release rates than those exposed to chemical cues of one male. Second, we measured CORT release rates of red-spotted newts in field enclosures with either a male-biased or a female-biased sex ratio. We predicted that females in the male-biased treatment would have higher CORT and T release rates than those in a female-biased treatment, owing to higher levels of male harassment. We also predicted that males would have higher CORT and T release rates in male-biased treatments due to higher levels of male-male competition. Females were not less active in response to chemical cues from more males over fewer males, but there was a positive relationship between female activity and CORT when they were exposed to the cues of three males. We also found that females, but not males, in the male-biased sex ratio treatment had higher CORT and T release rates than those in the female-biased treatment. Our results support the hypothesis that a male-biased sex ratio leads to a higher stress response, which may underlie the observed decrease in immune function and body condition in previous work exposing female red-spotted newts to a male-biased sex ratio. This study furthers our understanding of the mechanistic basis for costs associated with a male-biased sex ratio in a pond-breeding amphibian.
Department of Biology, Texas State University, 601 University Drive, San Marcos, TX 78666-4684, USA; Mountain Lake Biological Station, 240 Salt Pond Road, Pembroke, VA 24136, USA. Electronic address: aspbury@txstate.edu.
Andrea S
AS
Aspbury
Mountain Lake Biological Station, 240 Salt Pond Road, Pembroke, VA 24136, USA; Department of Biology, University of Richmond, 28 Westhampton Way, Richmond, VA, 23173, USA.
Kristine L
KL
Grayson
Mountain Lake Biological Station, 240 Salt Pond Road, Pembroke, VA 24136, USA.
Selamawit
S
Fantaye
Mountain Lake Biological Station, 240 Salt Pond Road, Pembroke, VA 24136, USA.
Ian
I
Nichols
Mountain Lake Biological Station, 240 Salt Pond Road, Pembroke, VA 24136, USA.
Miranda
M
Myers-Burton
Mountain Lake Biological Station, 240 Salt Pond Road, Pembroke, VA 24136, USA.
Xavier
X
Ortiz-Mangual
Department of Biology, Texas State University, 601 University Drive, San Marcos, TX 78666-4684, USA; Mountain Lake Biological Station, 240 Salt Pond Road, Pembroke, VA 24136, USA.
Caitlin R
CR
Gabor
D000704:Analysis of Variance; D000818:Animals; D015982:Bias; D002851:Chromatography, High Pressure Liquid; D003345:Corticosterone / Q000378:metabolism; D003463:Cues; D005260:Female; D008297:Male; D009673:Notophthalmus viridescens / Q000502:physiology*; D012727:Sex Characteristics; D012744:Sex Ratio; D012726:Sexual Behavior, Animal / Q000502:physiology*; D013312:Stress, Physiological / Q000502:physiology*; D013739:Testosterone / Q000378:metabolism; D013997:Time Factors
D013739:Testosterone; D003345:Corticosterone
Chemical cues; Coercive mating; Corticosterone; Sexual conflict; Testosterone; Water-borne hormone