Cells responding to surface structure of calcium phosphate ceramics for bone regeneration.
Surface structure largely affects the inductive bone-forming potential of calcium phosphate (CaP) ceramics in ectopic sites and bone regeneration in critical-sized bone defects. Surface-dependent osteogenic differentiation of bone marrow stromal cells (BMSCs) partially explained the improved bone-forming ability of submicron surface structured CaP ceramics. In this study, we investigated the possible influence of surface structure on different bone-related cells, which may potentially participate in the process of improved bone formation in CaP ceramics. Besides BMSCs, the response of human brain vascular pericytes (HBVP), C2C12 (osteogenic inducible cells), MC3T3-E1 (osteogenic precursors), SV-HFO (pre-osteoblasts), MG63 (osteoblasts) and SAOS-2 (mature osteoblasts) to the surface structure was evaluated in terms of cell proliferation, osteogenic differentiation and gene expression. The cells were cultured on tricalcium phosphate (TCP) ceramics with either micron-scaled surface structure (TCP-B) or submicron-scaled surface structure (TCP-S) for up to 14 days, followed by DNA, alkaline phosphatase (ALP) and quantitative polymerase chain reaction gene assays. HBVP were not sensitive to surface structure with respect to cell proliferation and osteogenic differentiation, but had downregulated angiogenesis-related gene expression (i.e. vascular endothelial growth factor) on TCP-S. Without additional osteogenic inducing factors, submicron-scaled surface structure enhanced ALP activity and osteocalcin gene expression of human (h)BMSCs and C2C12 cells, favoured the proliferation of MC3T3-E1, MG63 and SAOS-2, and increased ALP activity of MC3T3-E1 and SV-HFO. The results herein indicate that cells with osteogenic potency (either osteogenic inducible cells or osteogenic cells) could be sensitive to surface structure and responded to osteoinductive submicron-structured CaP ceramics in cell proliferation, ALP production or osteogenic gene expression, which favour bone regeneration. Copyright © 2017 John Wiley & Sons, Ltd.
Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, 7500AE Enschede, the Netherlands.|Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, China.
Jingwei
J
Zhang
Oral Implantology Center, Stomotology Hospital of Jinan, 250001, Jinan, China.
Lanying
L
Sun
Xpand Biotechnology BV, Bilthoven, the Netherlands.|Department of Biomaterials Science and Technology, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, 7500AE Enschede, the Netherlands.
Xiaoman
X
Luo
Xpand Biotechnology BV, Bilthoven, the Netherlands.
Davide
D
Barbieri
Xpand Biotechnology BV, Bilthoven, the Netherlands.|Department of Biomaterials Science and Technology, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, 7500AE Enschede, the Netherlands.|School of Engineering and Materials Science (SEMS), Queen Mary University of London, London, UK.
Joost D
JD
de Bruijn
Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, 7500AE Enschede, the Netherlands.|Complex Tissue Regeneration Department, MERLN Institute for Technology Inspired Regenerative Medicine, Maastricht University, 6229, ER, the Netherlands.
Clemens A
CA
van Blitterswijk
Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, 7500AE Enschede, the Netherlands.|Complex Tissue Regeneration Department, MERLN Institute for Technology Inspired Regenerative Medicine, Maastricht University, 6229, ER, the Netherlands.
Lorenzo
L
Moroni
Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, 7500AE Enschede, the Netherlands.|Xpand Biotechnology BV, Bilthoven, the Netherlands.|Complex Tissue Regeneration Department, MERLN Institute for Technology Inspired Regenerative Medicine, Maastricht University, 6229, ER, the Netherlands.|College of Physical Science and Technology, Sichuan University, Chengdu, China.
Huipin
H
Yuan
D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D051379:Mice; D010012:Osteogenesis / Q000187:drug effects*; D013499:Surface Properties
D018786:Bone Substitutes; D002130:Calcium Phosphates; C018392:tricalcium phosphate
bone regeneration; calcium phosphate ceramics; cell culture; cell proliferation; osteogenic differentiation; surface structure