Acclimation of shade-tolerant and light-resistant Tradescantia species to growth light: chlorophyll a fluorescence, electron transport, and xanthophyll content.
In this study, we have compared the photosynthetic characteristics of two contrasting species of Tradescantia plants, T. fluminensis (shade-tolerant species), and T. sillamontana (light-resistant species), grown under the low light (LL, 50-125 µmol photons m-2s-1) or high light (HL, 875-1000 µmol photons m-2s-1) conditions during their entire growth period. For monitoring the functional state of photosynthetic apparatus (PSA), we measured chlorophyll (Chl) a emission fluorescence spectra and kinetics of light-induced changes in the heights of fluorescence peaks at 685 and 740 nm (F685and F740). We also compared the light-induced oxidation of P700and assayed the composition of carotenoids in Tradescantia leaves grown under the LL and HL conditions. The analyses of slow induction of Chl a fluorescence (SIF) uncovered different traits in the LL- and HL-grown plants of ecologically contrasting Tradescantia species, which may have potential ecophysiological significance with respect to their tolerance to HL stress. The fluorometry and EPR studies of induction events in chloroplasts in situ demonstrated that acclimation of both Tradescantia species to HL conditions promoted faster responses of their PSA as compared to LL-grown plants. Acclimation of both species to HL also caused marked changes in the leaf anatomy and carotenoid composition (an increase in Violaxanthin + Antheraxantin + Zeaxanthin and Lutein pools), suggesting enhanced photoprotective capacity of the carotenoids in the plants grown in nature under high irradiance. Collectively, the results of the present work suggest that the mechanisms of long-term PSA photoprotection in Tradescantia are based predominantly on the light-induced remodeling of pigment-protein complexes in chloroplasts.
Faculty of Physics, M.V. Lomonosov Moscow State University, Moscow, 119991, Russia.
Vladimir I
VI
Mishanin
Faculty of Physics, M.V. Lomonosov Moscow State University, Moscow, 119991, Russia.
Boris V
BV
Trubitsin
Faculty of Physics, M.V. Lomonosov Moscow State University, Moscow, 119991, Russia.
Svetlana V
SV
Patsaeva
A.N. Belozersky Institute of Physical-Chemical Biology, M.V. Lomonosov Moscow State University, Moscow, 119234, Russia.|N.M. Emanuel Institute of Biochemical Physics, Moscow, 119334, Russia.
Vasily V
VV
Ptushenko
Faculty of Biology, M.V. Lomonosov Moscow State University, Moscow, 119234, Russia.
Alexei E
AE
Solovchenko
Faculty of Physics, M.V. Lomonosov Moscow State University, Moscow, 119991, Russia. an_tikhonov@mail.ru.|N.M. Emanuel Institute of Biochemical Physics, Moscow, 119334, Russia. an_tikhonov@mail.ru.
Alexander N
AN
Tikhonov
D000064:Acclimatization / Q000502:physiology / Q000528:radiation effects*; D002734:Chlorophyll / Q000378:metabolism*; D000077194:Chlorophyll A; D003624:Darkness; D004579:Electron Transport / Q000528:radiation effects; D007700:Kinetics; D008027:Light; D010084:Oxidation-Reduction; D018515:Plant Leaves / Q000378:metabolism / Q000528:radiation effects; D013050:Spectrometry, Fluorescence; D013997:Time Factors; D034823:Tradescantia / Q000254:growth & development* / Q000502:physiology / Q000528:radiation effects*; D024341:Xanthophylls / Q000378:metabolism*
D024341:Xanthophylls; D002734:Chlorophyll; D000077194:Chlorophyll A
Chlorophyll a fluorescence; Electron transport; Light-resistant species; Shade-tolerant species; Tradescantia; Xanthophyll cycle