Plasma cell treatment device Plasma-on-Chip: Monitoring plasma-generated reactive species in microwells.
We have developed a plasma cell treatment device called Plasma-on-Chip that enables the real-time monitoring of a single cell culture during plasma treatment. The device consists of three parts: 1) microwells for cell culture, 2) a microplasma device for generating reactive oxygen and nitrogen species (RONS) for use in cell treatment, and 3) through-holes (microchannels) that connect each microwell with the microplasma region for RONS delivery. Here, we analysed the delivery of the RONS to the liquid culture medium stored in the microwells. We developed a simple experimental set-up using a microdevice and applied in situ ultraviolet absorption spectroscopy with high sensitivity for detecting RONS in liquid. The plasma-generated RONS were delivered into the liquid culture medium via the through-holes fabricated into the microdevice. The RONS concentrations were on the order of 10-100 μM depending on the size of the through-holes. In contrast, we found that the amount of dissolved oxygen was almost constant. To investigate the process of RONS generation, we numerically analysed the gas flow in the through-holes. We suggest that the circulating gas flow in the through-holes promotes the interaction between the plasma (ionised gas) and the liquid, resulting in enhanced RONS concentrations.
Department of Electronic and Photonic Systems Engineering, Kochi University of Technology, Kami, Kochi, 782-8502 Japan.|Center for Nanotechnology, Research Institute of Kochi University of Technology, Kami, Kochi, 782-8502 Japan.
Jun-Seok
JS
Oh
Department of Advanced Science Engineering, Toyota Technological Institute, Nagoya, 468-8511 Japan.
Shinya
S
Kojima
Department of Advanced Science Engineering, Toyota Technological Institute, Nagoya, 468-8511 Japan.
Minoru
M
Sasaki
Department of Electronic and Photonic Systems Engineering, Kochi University of Technology, Kami, Kochi, 782-8502 Japan.|Center for Nanotechnology, Research Institute of Kochi University of Technology, Kami, Kochi, 782-8502 Japan.
Akimitsu
A
Hatta
Department of Advanced Science Engineering, Toyota Technological Institute, Nagoya, 468-8511 Japan.
Shinya
S
Kumagai
D018929:Cell Culture Techniques / Q000379:methods*; D006801:Humans; D056656:Lab-On-A-Chip Devices; D010950:Plasma Cells / Q000378:metabolism*; D058626:Plasma Gases / Q000378:metabolism*; D026361:Reactive Nitrogen Species / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*
D058626:Plasma Gases; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species