Generation of Spheres from Dental Epithelial Stem Cells.
Thein vitrothree-dimensional sphere model has already been established as an important tool in fundamental sciences. This model facilitates the study of a variety of biological processes including stem cell/niche functions and tissue responses to injury and drugs. Here we describe the complete protocol for thein vitroformation of spheres originated from the epithelium of rodent incisors. In addition, we show that in these spheres cell proliferation is maintained, as well as the expression of several key molecules characterizing stem cells such as Sox2 and p63. These epithelial dentospheres could be used as anin vitromodel system for stem cell research purposes.
Orofacial Development and Regeneration, Centre for Dental Medicine, Institute of Oral Biology, University of Zurich Zurich, Switzerland.
Despoina
D
Natsiou
Genomescan B.V. Leiden, Netherlands.
Zoraide
Z
Granchi
Orofacial Development and Regeneration, Centre for Dental Medicine, Institute of Oral Biology, University of Zurich Zurich, Switzerland.
Thimios A
TA
Mitsiadis
Orofacial Development and Regeneration, Centre for Dental Medicine, Institute of Oral Biology, University of Zurich Zurich, Switzerland.
Lucia
L
Jimenez-Rojo
3D culture; epithelial stem cells; incisor; mouse; regeneration; spheres; stem cell niche; tooth