Armc5 deletion causes developmental defects and compromises T-cell immune responses.
Armadillo repeat containing 5 (ARMC5) is a cytosolic protein with no enzymatic activities. Little is known about its function and mechanisms of action, except that gene mutations are associated with risks of primary macronodular adrenal gland hyperplasia. Here we map Armc5 expression by in situ hybridization, and generate Armc5 knockout mice, which are small in body size. Armc5 knockout mice have compromised T-cell proliferation and differentiation into Th1 and Th17 cells, increased T-cell apoptosis, reduced severity of experimental autoimmune encephalitis, and defective immune responses to lymphocytic choriomeningitis virus infection. These mice also develop adrenal gland hyperplasia in old age. Yeast 2-hybrid assays identify 16 ARMC5-binding partners. Together these data indicate that ARMC5 is crucial in fetal development, T-cell function and adrenal gland growth homeostasis, and that the functions of ARMC5 probably depend on interaction with multiple signalling pathways.
Centre de recherche (CR), Centre hospitalier de l'Université de Montréal (CHUM), 900 Rue Saint Denis, Montréal, Québec, Canada H2X 0A9.
Yan
Y
Hu
Centre de recherche (CR), Centre hospitalier de l'Université de Montréal (CHUM), 900 Rue Saint Denis, Montréal, Québec, Canada H2X 0A9.
Linjiang
L
Lao
Centre de recherche (CR), Centre hospitalier de l'Université de Montréal (CHUM), 900 Rue Saint Denis, Montréal, Québec, Canada H2X 0A9.
Jianning
J
Mao
Centre de recherche (CR), Centre hospitalier de l'Université de Montréal (CHUM), 900 Rue Saint Denis, Montréal, Québec, Canada H2X 0A9.
Wei
W
Jin
Centre de recherche (CR), Centre hospitalier de l'Université de Montréal (CHUM), 900 Rue Saint Denis, Montréal, Québec, Canada H2X 0A9.
Hongyu
H
Luo
Institut national de la recherche scientifique-Institut Armand-Frappier (INRS-IAF), 531 Boul. des Prairies, Laval, Québec, Canada H7V 1B7.
Tania
T
Charpentier
Centre de recherche (CR), Centre hospitalier de l'Université de Montréal (CHUM), 900 Rue Saint Denis, Montréal, Québec, Canada H2X 0A9.
Shijie
S
Qi
Centre de recherche (CR), Centre hospitalier de l'Université de Montréal (CHUM), 900 Rue Saint Denis, Montréal, Québec, Canada H2X 0A9.
Junzheng
J
Peng
Anatomic Pathology, AmeriPath Central Florida, 4225 Fowler Ave. Tampa, Orlando, Florida 33617, USA.
Bing
B
Hu
Cytochem Inc., 6465 Av Durocher, Outremont, Montréal, Québec, Canada H2V 3Z1.
Mieczyslaw Martin
MM
Marcinkiewicz
Institut national de la recherche scientifique-Institut Armand-Frappier (INRS-IAF), 531 Boul. des Prairies, Laval, Québec, Canada H7V 1B7.
Alain
0000-0002-7913-871X
A
Lamarre
Centre de recherche (CR), Centre hospitalier de l'Université de Montréal (CHUM), 900 Rue Saint Denis, Montréal, Québec, Canada H2X 0A9.|Nephrology Service, Centre hospitalier de l'Université de Montréal (CHUM), 900 Rue Saint Denis, Montréal, Québec, Canada H2X 0A9.
Jiangping
J
Wu
D000311:Adrenal Glands / Q000254:growth & development / Q000473:pathology*; D000818:Animals; D051186:Armadillo Domain Proteins / Q000235:genetics*; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D049109:Cell Proliferation / Q000235:genetics; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000175:diagnosis / Q000276:immunology*; D005260:Female; D047109:Fetal Development / Q000502:physiology*; D018095:Germ-Line Mutation; D006801:Humans; D006965:Hyperplasia / Q000235:genetics; D007111:Immunity, Cellular / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009154:Mutation; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology; D017384:Sequence Deletion; D012720:Severity of Illness Index; D013601:T-Lymphocytes / Q000502:physiology*; D018183:Transplantation Chimera / Q000276:immunology
D051186:Armadillo Domain Proteins; C000628964:Armc5 protein, mouse; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; C445081:myelin oligodendrocyte glycoprotein (35-55)