Major sulfonate transporter Soa1 in Saccharomyces cerevisiae and considerable substrate diversity in its fungal family.
Sulfate is a well-established sulfur source for fungi; however, in soils sulfonates and sulfate esters, especially choline sulfate, are often much more prominent. Here we show that Saccharomyces cerevisiae YIL166C(SOA1) encodes an inorganic sulfur (sulfate, sulfite and thiosulfate) transporter that also catalyses sulfonate and choline sulfate uptake. Phylogenetic analysis of fungal SOA1 orthologues and expression of 20 members in the sul1Δ sul2Δ soa1Δ strain, which is deficient in inorganic and organic sulfur compound uptake, reveals that these transporters have diverse substrate preferences for sulfur compounds. We further show that SOA2, a S. cerevisiae SOA1 paralogue found in S. uvarum, S. eubayanus and S. arboricola is likely to be an evolutionary remnant of the uncharacterized open reading frames YOL163W and YOL162W. Our work highlights the importance of sulfonates and choline sulfate as sulfur sources in the natural environment of S. cerevisiae and other fungi by identifying fungal transporters for these compounds.
Laboratory of Molecular Cell Biology, Department of Biology, KU Leuven, Institute of Botany and Microbiology Kasteelpark Arenberg 31, Flanders, B-3001 Leuven-Heverlee, Belgium.|Department of Molecular Microbiology, VIB, Kasteelpark Arenberg 31, Flanders, B-3001 Leuven-Heverlee, Belgium.
Sylvester
S
Holt
Laboratory of Molecular Cell Biology, Department of Biology, KU Leuven, Institute of Botany and Microbiology Kasteelpark Arenberg 31, Flanders, B-3001 Leuven-Heverlee, Belgium.|Department of Molecular Microbiology, VIB, Kasteelpark Arenberg 31, Flanders, B-3001 Leuven-Heverlee, Belgium.
Harish
H
Kankipati
Laboratory of Molecular Cell Biology, Department of Biology, KU Leuven, Institute of Botany and Microbiology Kasteelpark Arenberg 31, Flanders, B-3001 Leuven-Heverlee, Belgium.|Department of Molecular Microbiology, VIB, Kasteelpark Arenberg 31, Flanders, B-3001 Leuven-Heverlee, Belgium.
Stijn
S
De Graeve
Laboratory of Molecular Cell Biology, Department of Biology, KU Leuven, Institute of Botany and Microbiology Kasteelpark Arenberg 31, Flanders, B-3001 Leuven-Heverlee, Belgium.|Department of Molecular Microbiology, VIB, Kasteelpark Arenberg 31, Flanders, B-3001 Leuven-Heverlee, Belgium.
Griet
G
Van Zeebroeck
Laboratory of Molecular Cell Biology, Department of Biology, KU Leuven, Institute of Botany and Microbiology Kasteelpark Arenberg 31, Flanders, B-3001 Leuven-Heverlee, Belgium.|Department of Molecular Microbiology, VIB, Kasteelpark Arenberg 31, Flanders, B-3001 Leuven-Heverlee, Belgium.
Maria R
MR
Foulquié-Moreno
Carlsberg Research Laboratory, Gamle Carlsberg Vej 4, 1799 Copenhagen V, Denmark.
Stinus
0000-0001-5104-1054
S
Lindgreen
Laboratory of Molecular Cell Biology, Department of Biology, KU Leuven, Institute of Botany and Microbiology Kasteelpark Arenberg 31, Flanders, B-3001 Leuven-Heverlee, Belgium.|Department of Molecular Microbiology, VIB, Kasteelpark Arenberg 31, Flanders, B-3001 Leuven-Heverlee, Belgium.
Johan M
0000-0002-2626-0180
JM
Thevelein
D001692:Biological Transport; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000378:metabolism*; D010802:Phylogeny; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D012987:Soil / Q000737:chemistry; D013379:Substrate Specificity; D013457:Sulfur Compounds / Q000737:chemistry / Q000493:pharmacokinetics*
D026901:Membrane Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; D012987:Soil; D013457:Sulfur Compounds