Comparative study on the in vitro effects of Pseudomonas aeruginosa and seaweed alginates on human gut microbiota.
Alginates pertain to organic polysaccharides that have been extensively used in food- and medicine-related industries. The present study obtained alginates from an alginate overproducing Pseudomonas aeruginosa PAO1 mutant by screening transposon mutagenesis libraries. The interaction between bacterial and seaweed alginates and gut microbiota were further studied by using an in vitro batch fermentation system. Thin-layer chromatography (TLC) analysis indicated that both bacterial and seaweed alginates can be completely degraded by fecal bacteria isolated from study volunteers, indicating that a minor structural difference between bacterial and seaweed alginates (O-acetylation and lack of G-G blocks) didn't affect the digestion of alginates by human microbiota. Although, the digestion of bacterial and seaweed alginates was attributed to different Bacteroides xylanisolvens strains, they harbored similar alginate lyase genes. Genus Bacteroides with alginate-degrading capability were enriched in growth medium containing bacterial or seaweed alginates after in vitro fermentation. Short-chain fatty acid (SCFA) production in both bacterial and seaweed alginates was also comparable, but was significantly higher than the same medium using starch. In summary, the present study has isolated an alginate-overproducing P. aeruginosa mutant strain. Both seaweed and bacterial alginates were degraded by human gut microbiota, and their regulatory function on gut microbiota was similar.
State Key Laboratory of Breeding Base for Zhejiang Sustainable Pest, and Key laboratory for Food Microbial Technology of Zhejiang Province, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, P. R. China.|College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, China.
Shaofeng
S
Bai
State Key Laboratory of Breeding Base for Zhejiang Sustainable Pest, and Key laboratory for Food Microbial Technology of Zhejiang Province, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, P. R. China.
Huahai
H
Chen
State Key Laboratory of Breeding Base for Zhejiang Sustainable Pest, and Key laboratory for Food Microbial Technology of Zhejiang Province, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, P. R. China.
Liying
L
Zhu
State Key Laboratory of Breeding Base for Zhejiang Sustainable Pest, and Key laboratory for Food Microbial Technology of Zhejiang Province, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, P. R. China.
Wei
W
Liu
Departments of Biomedical Sciences, Pediatrics, Joan C. Edwards School of Medicine at Marshall University, Huntington, West Virginia, United States of America.|Progenesis Technologies, LLC, One John Marshall Drive, Robert C. Byrd Biotechnology Science Center, Huntington, West Virginia, United States of America.
Hongwei D
HD
Yu
State Key Laboratory of Breeding Base for Zhejiang Sustainable Pest, and Key laboratory for Food Microbial Technology of Zhejiang Province, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, P. R. China.
Xin
X
Wang
State Key Laboratory of Breeding Base for Zhejiang Sustainable Pest, and Key laboratory for Food Microbial Technology of Zhejiang Province, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, P. R. China.
Yeshi
Y
Yin
D000464:Alginates / Q000737:chemistry / Q000494:pharmacology*; D061249:Batch Cell Culture Techniques; D004251:DNA Transposable Elements; D005227:Fatty Acids / Q000096:biosynthesis; D005243:Feces / Q000382:microbiology; D005285:Fermentation; D000069196:Gastrointestinal Microbiome / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D054892:Metagenome; D056186:Metagenomics; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012624:Seaweed / Q000737:chemistry*
D000464:Alginates; D004251:DNA Transposable Elements; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S