Long-term nitrogen fertilization decreased the abundance of inorganic phosphate solubilizing bacteria in an alkaline soil.
Inorganic phosphate solubilizing bacteria (iPSB) are essential to facilitate phosphorus (P) mobilization in alkaline soil, however, the phylogenetic structure of iPSB communities remains poorly characterized. Thus, we use a reference iPSB database to analyze the distribution of iPSB communities based on 16S rRNA gene illumina sequencing. Additionally, a noval pqqC primer was developed to quantify iPSB abundance. In our study, an alkaline soil with 27-year fertilization treatment was selected. The percentage of iPSB was 1.10~2.87% per sample, and the dominant iPSB genera were closely related to Arthrobacter, Bacillus, Brevibacterium and Streptomyces. Long-term P fertilization had no significant effect on the abundance of iPSB communities. Rather than P and potassium (K) additions, long-term nitrogen (N) fertilization decreased the iPSB abundance, which was validated by reduced relative abundance of pqqC gene (pqqC/16S). The decreased iPSB abundance was strongly related to pH decline and total N increase, revealing that the long-term N additions may cause pH decline and subsequent P releases relatively decreasing the demands of the iPSB community. The methodology and understanding obtained here provides insights into the ecology of inorganic P solubilizers and how to manipulate for better P use efficiency.
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
Bang-Xiao
BX
Zheng
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.|Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg 1871, Denmark.
Xiu-Li
XL
Hao
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.
Kai
K
Ding
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
Guo-Wei
GW
Zhou
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
Qing-Lin
QL
Chen
State Key Lab Soil &Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
Jia-Bao
JB
Zhang
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.|State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Yong-Guan
YG
Zhu
D000468:Alkalies / Q000737:chemistry*; D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D005308:Fertilizers / Q000032:analysis*; D005798:Genes, Bacterial; D006863:Hydrogen-Ion Concentration; D009010:Monte Carlo Method; D009584:Nitrogen / Q000032:analysis*; D010710:Phosphates / Q000032:analysis*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry*
D000468:Alkalies; D005308:Fertilizers; D010710:Phosphates; D012336:RNA, Ribosomal, 16S; D012987:Soil; D009584:Nitrogen