Determination of the neuropharmacological drug nodakenin in rat plasma and brain tissues by liquid chromatography tandem mass spectrometry: Application to pharmacokinetic studies.
A rapid and sensitive liquid chromatography tandem mass spectrometry detection using selected reaction monitoring in positive ionization mode was developed and validated for the quantification of nodakenin in rat plasma and brain. Pareruptorin A was used as internal standard. A single step liquid-liquid extraction was used for plasma and brain sample preparation. The method was validated with respect to selectivity, precision, accuracy, linearity, limit of quantification, recovery, matrix effect and stability. Lower limit of quantification of nodakenin was 2.0 ng/mL in plasma and brain tissue homogenates. Linear calibration curves were obtained over concentration ranges of 2.0-1000 ng/mL in plasma and brain tissue homogenates for nodakenin. Intra-day and inter-day precisions (relative standard deviation, RSD) were <15% in both biological media. This assay was successfully applied to plasma and brain pharmacokinetic studies of nodakenin in rats after intravenous administration.
Department of Pharmacy, the First Hospital of Jilin University, Changchun, People's Republic of China.
Yingshi
Y
Song
Department of Pharmacy, the First Hospital of Jilin University, Changchun, People's Republic of China.
Huiyu
H
Yan
Department of Pharmacy, the First Hospital of Jilin University, Changchun, People's Republic of China.
Jingbo
J
Xu
Department of Pathology, the First Hospital of Jilin University, Changchun, People's Republic of China.
Hongxi
http://orcid.org/0000-0001-6064-6194
H
Ma
D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D003374:Coumarins / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D004355:Drug Stability; D005960:Glucosides / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D016014:Linear Models; D059625:Liquid-Liquid Extraction; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D003374:Coumarins; D005960:Glucosides; C471579:nodakenin
brain tissue; liquid chromatography tandem mass spectrometry; nodakenin; pharmacokinetics; rat plasma