miR-450a-5p within rat adipose tissue exosome-like vesicles promotes adipogenic differentiation by targeting WISP2.
Adipose tissue is an active endocrine organ that can secrete a wide number of factors to regulate adipogenesis via paracrine signals. In addition to soluble proteins in adipose tissue, microRNAs (miRNAs) enriched in extracellular vesicles (EVs), such as exosomes or microvesicles, could modulate intercellular communications. In this study, we demonstrated that exosome-like vesicles derived from adipose tissue (Exo-AT) were internalized by adipose tissue-derived stem cells (ADSCs), and that these, in turn, induced adipogenesis. High-throughput sequencing showed that 45 miRNAs were enriched in Exo-AT, and 31.11% of them were associated with adipogenesis, compared with ADSC-derived exosome-like vesicles (Exo-ADSC). miR-450a-5p, one of the most abundant miRNAs in Exo-AT, was a proadipogenic miRNA. Further study demonstrated that miR-450a-5p promoted adipogenesis through repressing expression of WISP2 by targeting its 3' untranslated region. Additionally, Exo-AT could also downregulate the expression of WISP2, while miR-450a-5p inhibitor reversed this effect. Moreover, inhibition of miR-450a-5p impaired adipogenesis mediated by exosome-like vesicles. In conclusion, Exo-AT mediates adipogenic differentiation through a mechanism involving transfer of miR-450a-5p.
State Key Laboratory of Oral Disease, West China School of Stomatology, Sichuan University, Chengdu 610041, China.|National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, No. 14, 3rd Sec., Ren Min Nan Road, Chengdu, Sichuan Province 610041, P.R. China.|Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Yan
0000-0003-3079-9960
Y
Zhang
State Key Laboratory of Oral Disease, West China School of Stomatology, Sichuan University, Chengdu 610041, China yumei925@hotmail.com drtwd@sina.com.|National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, No. 14, 3rd Sec., Ren Min Nan Road, Chengdu, Sichuan Province 610041, P.R. China.
Mei
0000-0003-0306-6817
M
Yu
State Key Laboratory of Oral Disease, West China School of Stomatology, Sichuan University, Chengdu 610041, China.|National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, No. 14, 3rd Sec., Ren Min Nan Road, Chengdu, Sichuan Province 610041, P.R. China.|Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Minjia
0000-0002-2613-7588
M
Dai
State Key Laboratory of Oral Disease, West China School of Stomatology, Sichuan University, Chengdu 610041, China.|National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, No. 14, 3rd Sec., Ren Min Nan Road, Chengdu, Sichuan Province 610041, P.R. China.|Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Chang
0000-0002-6049-1193
C
Chen
State Key Laboratory of Oral Disease, West China School of Stomatology, Sichuan University, Chengdu 610041, China.|National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, No. 14, 3rd Sec., Ren Min Nan Road, Chengdu, Sichuan Province 610041, P.R. China.|Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Qi
0000-0003-3048-1813
Q
Tang
State Key Laboratory of Oral Disease, West China School of Stomatology, Sichuan University, Chengdu 610041, China.|National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, No. 14, 3rd Sec., Ren Min Nan Road, Chengdu, Sichuan Province 610041, P.R. China.|Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Wei
W
Jing
State Key Laboratory of Oral Disease, West China School of Stomatology, Sichuan University, Chengdu 610041, China.|National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, No. 14, 3rd Sec., Ren Min Nan Road, Chengdu, Sichuan Province 610041, P.R. China.|Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Hang
0000-0001-6916-0887
H
Wang
State Key Laboratory of Oral Disease, West China School of Stomatology, Sichuan University, Chengdu 610041, China yumei925@hotmail.com drtwd@sina.com.|National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, No. 14, 3rd Sec., Ren Min Nan Road, Chengdu, Sichuan Province 610041, P.R. China.|Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Weidong
0000-0003-0175-1132
W
Tian
D050156:Adipogenesis / Q000235:genetics; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D055511:CCN Intercellular Signaling Proteins / Q000378:metabolism*; D004705:Endocytosis; D055354:Exosomes / Q000378:metabolism* / Q000648:ultrastructure; D020869:Gene Expression Profiling; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D017207:Rats, Sprague-Dawley; D012097:Repressor Proteins / Q000378:metabolism*
D055511:CCN Intercellular Signaling Proteins; C115044:CCN5 protein, rat; C000621796:MIRN450 microRNA, rat; D035683:MicroRNAs; D012097:Repressor Proteins
Adipogenesis; Adipose tissue; Exosome-like vesicle; WISP2; miR-450a-5p