Gene analysis of two cases with CisAB/B blood subgroup.
OBJECTIVE
To identify the precise ABO blood type subgroups with genotyping.


METHODS
We screened ABO blood type from voluntary blood donors in our blood collection center. Samples were first examined with a routine serological method. Samples with ambiguous results were further examined with genotyping to identify ABO subgroups.


RESULTS
Two samples identified as AweakB by serology were considered to be A/B and B/B by PCR-SSP. However, the gene sequencing results revealed the precise subgroup to be CisAB01/B101and CisAB05/B101, respectively.


CONCLUSIONS
It may be difficult to identify non-typical AB patients with a routine serological method. Genotyping is a more precise method to identify blood subgroups.
Blood Center of Jilin Province, Changchun 130033, China.
Li-Xin
LX
Jiao
Blood Center of Jilin Province, Changchun 130033, China.
Jiang-Hong
JH
Yu
Blood Center of Jilin Province, Changchun 130033, China.
Xiao-Li
XL
Yu
Blood Center of Jilin Province, Changchun 130033, China. Electronic address: 949278220@qq.com.
Lin
L
Chen
Blood Center of Jilin Province, Changchun 130033, China.
Yu
Y
Han
Blood Center of Jilin Province, Changchun 130033, China.
Fan
F
Yang
D000017:ABO Blood-Group System / Q000235:genetics*; D000328:Adult; D001782:Blood Donors; D005838:Genotype; D060005:Genotyping Techniques; D006801:Humans; D008297:Male; D008875:Middle Aged
D000017:ABO Blood-Group System
ABO blood subgroup; CisAB blood type