First Report of Dieback Caused byLasiodiplodia theobromaein Strawberry Plants in Korea.
Dieback in strawberry (Seolhyang cultivar) was first observed during the nursery season (June to September) in the Nonsan area of Korea in the years 2012 and 2013. Initial disease symptoms included dieback on runners, as well as black rot on roots, followed by wilting and eventually blackened, necrotic discoloration in the crowns of daughter plants. A fungus isolated from the diseased roots, runners, and crowns is close toLasiodiplodia theobromaebased on morphological characteristics. Analysis of a combined dataset assembled from sequences of the internal transcribed spacer and translation elongation factor 1-alpha genes grouped nine fungal isolates with the type strain ofL. theobromae. The isolates showed strong pathogenicity on strawberry cultivars Kumhyang, Seolhyang, and Akihimae, fulfilling Koch's postulates. Based on these results, the pathogen responsible for dieback on strawberry plants in Korea was identified asL. theobromae.
Nonsan Strawberry Experiment Station, Fruit and Vegetable Research Center, Chungnam ARES, Nonsan 32914, Korea.
Myeong Hyeon
MH
Nam
School of Biological Sciences, Seoul National University, Seoul 08826, Korea.
Myung Soo
MS
Park
Nonsan Strawberry Experiment Station, Fruit and Vegetable Research Center, Chungnam ARES, Nonsan 32914, Korea.
Hyun Sook
HS
Kim
Nonsan Strawberry Experiment Station, Fruit and Vegetable Research Center, Chungnam ARES, Nonsan 32914, Korea.
Tae Il
TI
Kim
Nonsan Strawberry Experiment Station, Fruit and Vegetable Research Center, Chungnam ARES, Nonsan 32914, Korea.
Eun Mo
EM
Lee
Nonsan Agricultural Technology, Nonsan 32914, Korea.
Jong Dae
JD
Park
Department of Applied Biology, Chungnam National University, Daejeon 34134, Korea.
Hong Gi
HG
Kim
Dieback; ITS; Lasiodiplodia; Strawberry; tef1