PVX-tolerant potato development using a nucleic acid-hydrolyzing recombinant antibody.
3D8 scFv, a catalytic recombinant antibody developed in the MRL mouse, exhibits nucleic acid-hydrolyzing activity. Previous studies have demonstrated that tobacco plants harboring 3D8 scFv antibodies showed broad-spectrum resistance to infection by both DNA and RNA viruses. In this study, potatoes were transformed with the 3D8 scFv gene and screened by potato virus X (PVX) challenge. Starting with the T0 and T1 potato lines, PVX-tolerant T1 potatoes were identified in the field and characterized by ELISA and RT-PCR analysis. T2 potatoes were propagated for T3 generation and additional virus challenges in the field, and 44% of the 3D8 scFv T3 transgenic potatoes grown in GMO fields were found to be tolerant to PVX infection. Tubers from PVX-tolerant T3 lines were 60% bigger and 24% heavier, compared with tubers from PVX-susceptible transgenic lines and wild-type potatoes. Three-step virus challenge experiments and molecular characterization techniques were used for plants grown in growth chambers or fields to identify 3D8 scFv-transgenic, PVX-tolerant potatoes. These studies also revealed that the viral tolerance enabled by 3D8 scFv persisted during asexual propagation.
J-G
JG
Yang
K-H
KH
Hwang
E-J
EJ
Kil
J
J
Park
S
S
Cho
Y-G
YG
Lee
C-K
CK
Auh
Y
Y
Rhee
S
S
Lee
D000914:Antibodies, Viral; D020022:Genetic Predisposition to Disease; D010935:Plant Diseases / Q000235:genetics / Q000821:virology*; D030821:Plants, Genetically Modified; D017800:Potyvirus; D011994:Recombinant Proteins; D011198:Solanum tuberosum / Q000235:genetics* / Q000821:virology*; D014170:Transformation, Genetic
D000914:Antibodies, Viral; D011994:Recombinant Proteins
potato virus X; 3D8 scFv; agrobacterium-mediated transformation; transgenic potato; PVX tolerance.