The PeptideAtlas of the Domestic Laying Hen.
Proteomics-based biological research is greatly expanded by high-quality mass spectrometry studies, which are themselves enabled by access to quality mass spectrometry resources, such as high-quality curated proteome data repositories. We present a PeptideAtlas for the domestic chicken, containing an extensive and robust collection of chicken tissue and plasma samples with substantial value for the chicken proteomics community for protein validation and design of downstream targeted proteome quantitation. The chicken PeptideAtlas contains 6646 canonical proteins at a protein FDR of 1.3%, derived from ∼100 000 peptides at a peptide level FDR of 0.1%. The rich collection of readily accessible data is easily mined for the purposes of data validation and experimental planning, particularly in the realm of developing proteome quantitation workflows. Herein we demonstrate the use of the atlas to mine information on common chicken acute-phase proteins and biomarkers for cancer detection research, as well as their localization and polymorphisms. This wealth of information will support future proteome-based research using this highly important agricultural organism in pursuit of both chicken and human health outcomes.
W.M. Keck FTMS Laboratory for Human Health Research, Department of Chemistry, North Carolina State University , Raleigh, North Carolina 27695, United States.
James
J
McCord
Institute for Systems Biology , Seattle, Washington 98109, United States.
Zhi
Z
Sun
Institute for Systems Biology , Seattle, Washington 98109, United States.
Eric W
EW
Deutsch
Institute for Systems Biology , Seattle, Washington 98109, United States.
Robert L
RL
Moritz
W.M. Keck FTMS Laboratory for Human Health Research, Department of Chemistry, North Carolina State University , Raleigh, North Carolina 27695, United States.
David C
0000-0003-2216-499X
DC
Muddiman
D000209:Acute-Phase Proteins / Q000032:analysis; D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D002645:Chickens; D057225:Data Mining; D005260:Female; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*
D000209:Acute-Phase Proteins; D014408:Biomarkers, Tumor; D020543:Proteome
PeptideAtlas; biomarkers; chicken; ovarian cancer