Microtubule stabilisers docetaxel and paclitaxel reduce spindle damage and maintain the developmental competence of in vitro-mature bovine oocytes during vitrification.
This study compared the efficacy of docetaxel (DT) and paclitaxel (PT) in reducing spindle damage during vitrification and maintaining the developmental competence of in vitro-matured (IVM) bovine oocytes after vitrification and warming. Pretreatment of IVM oocytes with 0.05µM DT for 30min before vitrification resulted in significantly higher (P<0.05) rates of oocyte survival and cleavage after IVF, as well as subsequent blastocyst rates on Days 7-9 and hatching on Days 8-9, compared with oocytes pretreated with 1.0µM PT before vitrification or those vitrified without pretreatment. When nuclear status and spindle morphology of vitrified oocytes were assess after warming by immunostaining, DT pretreatment before vitrification resulted in a significantly higher (P<0.05) percentage of oocytes at the MII stage with a normal, intact spindle compared with PT pretreatment or no pretreatment, but the percentage of MII oocytes was still significantly lower (P<0.05) than in the control group. Pretreatment of IVM bovine oocytes with 0.05µM DT or 1.0µM PT for 30min before vitrification reduces spindle damage to the same extent, without side effects on fertilisation and development. Pretreatment with 0.05µM DT improved the developmental competence of vitrified-warmed oocytes to a greater degree than 1.0µM PT pretreatment.
Faculty of Agriculture, Princess of Naradhiwas University, Narathiwat 96000, Thailand.
Jakkhaphan
J
Pitchayapipatkul
Animal Breeding and Reproduction Research Division, National Agriculture and Food Research Organisation (NARO) Institute of Livestock and Grassland Science, 2 Ikenodai, Tsukuba, Ibaraki 305-0901, Japan.
Tamás
T
Somfai
Animal Breeding and Reproduction Research Division, National Agriculture and Food Research Organisation (NARO) Institute of Livestock and Grassland Science, 2 Ikenodai, Tsukuba, Ibaraki 305-0901, Japan.
Satoko
S
Matoba
Embryo Technology and Stem Cell Research Center, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Rangsan
R
Parnpai
Food and Fertilizer Technology Center, Taipei 10648, Taiwan.
Takashi
T
Nagai
Animal Breeding and Reproduction Research Division, National Agriculture and Food Research Organisation (NARO) Institute of Livestock and Grassland Science, 2 Ikenodai, Tsukuba, Ibaraki 305-0901, Japan.
Masaya
M
Geshi
Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand.
Thevin
T
Vongpralub
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000187:drug effects; D015925:Cryopreservation; D000077143:Docetaxel; D005260:Female; D059471:In Vitro Oocyte Maturation Techniques / Q000662:veterinary; D009865:Oocytes / Q000187:drug effects*; D017239:Paclitaxel / Q000494:pharmacology*; D008941:Spindle Apparatus / Q000187:drug effects*; D043823:Taxoids / Q000494:pharmacology*; D050257:Tubulin Modulators / Q000494:pharmacology*; D058989:Vitrification
D043823:Taxoids; D050257:Tubulin Modulators; D000077143:Docetaxel; D017239:Paclitaxel