Expression and activation of platelet-derived growth factor β receptor, mitogen-activated protein/extracellular signal-regulated kinase kinase (MEK) and extracellular signal-regulated kinase (ERK) in canine mammary tumours.
Canine mammary tumours are frequent neoplasms mostly affecting intact female dogs, for which no 100% efficient therapy is available. Platelet derived growth factor β receptor (PDGFβR) is a tyrosine kinase receptor (TKR) with a potential role in human breast cancer and a series of canine tumours. In this study we demonstrated, for the first time, expression of PDGFβR and its downstream transduction molecules, mitogen-activated protein/extracellular signal-regulated kinase kinase (MEK) and extracellular signal-regulated kinase (ERK), as well as their activated forms in canine mammary tumours by both biochemical analysis and immunohistochemistry. PDGFβR was expressed and hyperphosphorylated in the majority of tumour samples and tumour derived cell lines. Additionally, both MEK and ERK were expressed and activated in cell lines as well as biopsies. TKR inhibitors (TKRi) are currently under investigation as possible therapy in human breast and several canine tumours, thus our in vivo and in vitro findings pave the way for future studies aimed at establishing a potential therapeutic employment of TKRi for the treatment of canine mammary cancer.
Department of Veterinary medicine and Animal productions, Via Veterinaria, 1, 80137, Napoli, Italy. Electronic address: gennaro.altamura@unina.it.
Gennaro
G
Altamura
Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, Napoli, Italy. Electronic address: barbara.degliuberti@cert.izsmportici.it.
Barbara Degli
BD
Uberti
Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, Napoli, Italy. Electronic address: giorgio.galiero@cert.izsmportici.it.
Giorgio
G
Galiero
Department of Veterinary medicine and Animal productions, Via Veterinaria, 1, 80137, Napoli, Italy. Electronic address: manuela.martano@unina.it.
Manuela
M
Martano
Department of Veterinary medicine and Animal productions, Via Veterinaria, 1, 80137, Napoli, Italy. Electronic address: divas3@hotmal.it.
Antonella
A
Pirro
Department of Veterinary medicine and Animal productions, Via Veterinaria, 1, 80137, Napoli, Italy. Electronic address: marco.russo@unina.it.
Marco
M
Russo
Department of Veterinary medicine and Animal productions, Via Veterinaria, 1, 80137, Napoli, Italy. Electronic address: borzacch@unina.it.
Giuseppe
G
Borzacchiello
D000818:Animals; D045744:Cell Line, Tumor; D004283:Dog Diseases / Q000235:genetics* / Q000378:metabolism; D004285:Dogs; D048049:Extracellular Signal-Regulated MAP Kinases / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D015674:Mammary Neoplasms, Animal / Q000235:genetics* / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics* / Q000378:metabolism; D010766:Phosphorylation; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction
D020797:Receptor, Platelet-Derived Growth Factor beta; D048049:Extracellular Signal-Regulated MAP Kinases; D020928:Mitogen-Activated Protein Kinases
ERK; MEK; Oncology; PDGFβR; TKR inhibitors