Immunocytoexpression profile of ProExC in smears interpreted as ASC-US, ASC-H, and cervical intraepithelial lesion.
OBJECTIVE
We aimed to investigate the immunocytoexpression profiles of a novel assay ProEx C for topoisomerase II alpha (TOP2A) and minichromosome maintenance protein 2 (MCM2) in abnormal interpreted smears.


METHODS
Screening programs with Papanicolaou smear and high risk group human papilloma virus testing have yielded a dramatic reduction of cervical cancer incidence. However, both of these tests have limited specificity for the detection of clinically significant cervical high grade lesions. ProEx C for topoisomerase II alpha (TOP2A) and minichromosome maintenance protein 2 (MCM2) has been considered to have tight association with high grade intraepithelial lesions.


METHODS
A total number of 54 SurePath cervical cytology specimens of patients previously interpreted as atypical squamous cells-undetermined significance (ASC-US), atypical squamous cells-cannot exclude high grade squamous intraepithelial lesion (ASC-H), low grade squamous intraepithelial lesion (LSIL), and high grade squamous intraepithelial lesion (HSIL) were included in our study.


CONCLUSIONS
ProEx C was positive in 14 of HSILs (100%), 3 of 19 LSILs (16%), 2 of 4 ASC-Hs, and none of ASC-USs (0%). The ProEx C test showed very intense nuclear staining in all cytologically abnormal cells. Further studies are indicated to evaluate the diagnostic role of ProEx C.
Department of Pathology, Faculty of Medicine, Bezmialem Foundation University, İstanbul, Turkey.
Zeynep
Z
Tosuner
Department of Pathology, Faculty of Medicine, Medipol University, İstanbul, Turkey.
İlknur
İ
Türkmen
Department of Pathology, Faculty of Medicine, Bezmialem Foundation University, İstanbul, Turkey.
Sema
S
Arici
Department of Pathology, Faculty of Medicine, Bezmialem Foundation University, İstanbul, Turkey.
Cavide
C
Sönmez
Department of Pathology, Faculty of Medicine, Bezmialem Foundation University, İstanbul, Turkey.
Seval
S
Turna
Department of Pathology, Faculty of Medicine, Bezmialem Foundation University, İstanbul, Turkey.
Öykü
Ö
Onaran
ASC-H; ASC-US; HSIL; LSIL; ProEx C; immunocytochemistry