Consumption of protein-enriched milk has minor effects on inflammation in older adults-A 12-week double-blind randomized controlled trial.
Aging is associated with increased levels of circulating inflammatory markers and reduced muscle mass and strength.



We investigated whether intake of protein-enriched milk for 12 weeks would influence markers of inflammation among adults ≥70years of age with reduced physical strength.



In a double-blind randomized controlled intervention study, subjects were randomly allocated into two groups, receiving a protein-enriched milk (2×20g protein/d, n=14, mean (±SD) age 76.9±4.9 yrs) or an isocaloric carbohydrate drink (n=17, age 77.7±4.8 yrs) for 12 weeks. We measured serum and mRNA expression levels of inflammatory markers in PBMCs.



Significant differences in the mRNA expression of nuclear receptor subfamily, group H, member 3 (NR1H3, encoding the LXRα transcription factor) and interferon gamma (INFG) were observed between groups. The mRNA level of TNFRSF1A was significantly reduced, while the mRNA level of dipeptidyl-peptidase 4 (DPP4) was significantly increased, in the control group. The serum level of TNFα increased significantly in the control group, while sTNFRSF1A increased significantly in both groups, but with no significant differences between groups.



Consumption of a low-fat, protein-enriched milk for 12 weeks had minor effects on inflammatory related markers in older adults compared to an isocaloric carbohydrate drink.
Department of Nutrition, Institute of Basic Medical Sciences, P.O. Box 1046, Blindern, 0317 University of Oslo, Norway; TINE SA, Centre for Research and Development, P.O. Box 7, Kalbakken, 0902 Oslo, Norway. Electronic address: g.o.gjevestad@medisin.uio.no.
Gyrd O
GO
Gjevestad
Department of Nutrition, Institute of Basic Medical Sciences, P.O. Box 1046, Blindern, 0317 University of Oslo, Norway. Electronic address: inger.ottestad@medisin.uio.no.
Inger
I
Ottestad
TINE SA, Centre for Research and Development, P.O. Box 7, Kalbakken, 0902 Oslo, Norway. Electronic address: anne.sofie.biong@tine.no.
Anne Sofie
AS
Biong
Department of Nutrition, Institute of Basic Medical Sciences, P.O. Box 1046, Blindern, 0317 University of Oslo, Norway; Department of Hematology, Oslo University Hospital, P.O. Box 4950 Nydalen, 0424 Oslo, Norway. Electronic address: poiversen@hotmail.com.
Per Ole
PO
Iversen
Department of Nutrition, Institute of Basic Medical Sciences, P.O. Box 1046, Blindern, 0317 University of Oslo, Norway; Lipid Clinic, Oslo University Hospital, P.O. Box 4950 Nydalen, 0424 Oslo, Norway. Electronic address: kjetil.retterstol@medisin.uio.no.
Kjetil
K
Retterstøl
Department of Physical Performance, Norwegian School of Sport Sciences, P.B. 4104 U.S., 0806 Oslo, Norway. Electronic address: truls.raastad@nih.no.
Truls
T
Raastad
Department of Nutrition, Institute of Basic Medical Sciences, P.O. Box 1046, Blindern, 0317 University of Oslo, Norway. Electronic address: b.s.skalhegg@medisin.uio.no.
Bjørn S
BS
Skålhegg
Department of Nutrition, Institute of Basic Medical Sciences, P.O. Box 1046, Blindern, 0317 University of Oslo, Norway. Electronic address: smulven@medisin.uio.no.
Stine M
SM
Ulven
Department of Nutrition, Institute of Basic Medical Sciences, P.O. Box 1046, Blindern, 0317 University of Oslo, Norway; National Advisory Unit on Familial Hypercholesterolemia, Department of Endocrinology, Morbid Obesity and Preventive Medicine, Oslo University Hospital, P.O. Box 4950 Nydalen, 0424 Oslo, Norway. Electronic address: k.b.holven@medisin.uio.no.
Kirsten B
KB
Holven
D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000097:blood*; D000818:Animals; D015415:Biomarkers / Q000097:blood; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood*; D000071518:Liver X Receptors / Q000097:blood*; D008297:Male; D008892:Milk; D018482:Muscle, Skeletal / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000097:blood*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
C091514:BABAM2 protein, human; D015415:Biomarkers; D018836:Inflammation Mediators; D000071518:Liver X Receptors; C000605213:NR1H3 protein, human; D009419:Nerve Tissue Proteins; D014409:Tumor Necrosis Factor-alpha
High-protein; Inflammation; Milk protein; Older adults; Peripheral blood mononuclear cells; mRNA