Serum from postmenopausal women treated with a by-product of olive-oil extraction process stimulates osteoblastogenesis and inhibits adipogenesis in human mesenchymal stem-cells (MSC).
Aging may enhance both oxidative stress and bone-marrow mesenchymal stem-cell (MSC) differentiation into adipocytes. That reduces osteoblastogenesis, thus favoring bone-mass loss and fracture, representing an important worldwide health-issue, mainly in countries with aging populations. Intake of antioxidant products may help to retain bone-mass density. Interestingly, a novel olive-pomace physical treatment to generate olive oil also yields by-products rich in functional antioxidants. Thus, diet of postmenopausal women was supplemented for two months with one of such by-products (distillate 6; D6), being rich in squalene. After treatment, serum from such women showed reduced both lipidic peroxidation and oxidized low-density lipoprotein (LDL). Besides, vitamin E and coenzyme Q10 levels increased. Furthermore, culture medium containing 10% of such serum both increased osteoblastogenesis and reduced adipogenesis in human MSC from bone marrow. Therefore, highly antioxidant by-products like D6 may represent a relevant source for development of functional products, for both prevention and treatment of degenerative pathologies associated with aging, like osteoporosis.
Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Unidad de Gestión Clínica (UGC) de Endocrinología y Nutrición, Hospital Universitario Reina Sofía, Universidad de Córdoba, Avda. Menéndez Pidal s/n, 14004 Córdoba, Spain; RETICEF & CIBER de Fragilidad y Envejecimento Saludable (CIBERFES), Spain.
Antonio
A
Casado-Díaz
Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Unidad de Gestión Clínica (UGC) de Endocrinología y Nutrición, Hospital Universitario Reina Sofía, Universidad de Córdoba, Avda. Menéndez Pidal s/n, 14004 Córdoba, Spain; Dep. Bioquímica y Biología Molecular, Facultad de Medicina y Enfermería, Universidad de Córdoba, Avda. Menéndez Pidal s/n, 14004 Córdoba, Spain.
Isaac
I
Túnez-Fiñana
Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Unidad de Gestión Clínica (UGC) de Endocrinología y Nutrición, Hospital Universitario Reina Sofía, Universidad de Córdoba, Avda. Menéndez Pidal s/n, 14004 Córdoba, Spain.
José María
JM
Mata-Granados
Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (CSIC), Campus Universitario Pablo de Olavide E46, Ctra. de Utrera km 1, 41013 Sevilla, Spain.
María Victoria
MV
Ruiz-Méndez
Dep. Bioquímica y Biología Molecular, Universidad de Córdoba, Campus de Excelencia Internacional Agroalimentario (ceiA3), Campus Rabanales C6-1-E17, 14071 Córdoba, Spain; RETICEF & CIBER de Fragilidad y Envejecimento Saludable (CIBERFES), Spain.
Gabriel
G
Dorado
Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Unidad de Gestión Clínica (UGC) de Endocrinología y Nutrición, Hospital Universitario Reina Sofía, Universidad de Córdoba, Avda. Menéndez Pidal s/n, 14004 Córdoba, Spain; RETICEF & CIBER de Fragilidad y Envejecimento Saludable (CIBERFES), Spain.
María Concepción
MC
Romero-Sánchez
UGC de Cardiología, Hospital Universitario Virgen de Valme, Ctra. Cádiz s/n, 41014 Sevilla, Spain.
Cristina
C
Navarro-Valverde
Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Unidad de Gestión Clínica (UGC) de Endocrinología y Nutrición, Hospital Universitario Reina Sofía, Universidad de Córdoba, Avda. Menéndez Pidal s/n, 14004 Córdoba, Spain; RETICEF & CIBER de Fragilidad y Envejecimento Saludable (CIBERFES), Spain. Electronic address: jmquesada@uco.es.
José Manuel
JM
Quesada-Gómez
D050156:Adipogenesis / Q000187:drug effects*; D000368:Aged; D000375:Aging; D002478:Cells, Cultured; D019587:Dietary Supplements; D005260:Female; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D008875:Middle Aged; D000069463:Olive Oil / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology; D010012:Osteogenesis / Q000187:drug effects*; D010024:Osteoporosis / Q000473:pathology; D017698:Postmenopause / Q000097:blood*
D008077:Lipoproteins, LDL; D000069463:Olive Oil; C099252:oxidized low density lipoprotein
Adipocytes; Aging; Mesenchymal stem-cells; Olive-pomace oil distillate; Osteoblasts; Osteoporosis