Elastic modulus and hydraulic permeability of MDCK monolayers.
The critical role of cell mechanics in tissue health has led to the development of many in vitro methods that measure the elasticity of the cytoskeleton and whole cells, yet the connection between these local cell properties and bulk measurements of tissue mechanics remains unclear. To help bridge this gap, we have developed a monolayer indentation technique for measuring multi-cellular mechanics in vitro. Here, we measure the elasticity of cell monolayers and uncover the role of fluid permeability in these multi-cellular systems, finding that the resistance of fluid transport through cells controls their force-response at long times.
Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL, United States.
K D
KD
Schulze
Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL, United States.
S M
SM
Zehnder
Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL, United States.
J M
JM
Urueña
Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL, United States.
T
T
Bhattacharjee
Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL, United States; Department of Material Science and Engineering, University of Florida, Gainesville, FL, United States.
W G
WG
Sawyer
Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL, United States; J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, United States; Institute for Cell Engineering and Regenerative Medicine, University of Florida, Gainesville, FL, United States. Electronic address: t.e.angelini@ufl.edu.
T E
TE
Angelini
D000818:Animals; D018929:Cell Culture Techniques; D002463:Cell Membrane Permeability; D003599:Cytoskeleton / Q000502:physiology*; D004285:Dogs; D055119:Elastic Modulus / Q000502:physiology; D004548:Elasticity; D061985:Madin Darby Canine Kidney Cells; D008853:Microscopy / Q000379:methods
Cell mechanics; Contact mechanics; Indentation; Mesoscale; Permeability