microRNA-200a-3p enhances mitochondrial elongation by targeting mitochondrial fission factor.
Mitochondria play pivotal roles in the ATP production, apoptosis and generation of reactive oxygen species. Although dynamic regulation of mitochondria morphology is a critical step to maintain cellular homeostasis, the regulatory mechanisms are not yet fully elucidated. In this study, we identified miR-200a-3p as a novel regulator of mitochondrial dynamics by targeting mitochondrial fission factor (MFF). We demonstrated that the ectopic expression of miR-200a-3p enhanced mitochondrial elongation, mitochondrial ATP synthesis, mitochondrial membrane potential and oxygen consumption rate. These results indicate that miR-200a-3p positively regulates mitochondrial elongation by downregulating MFF expression. [BMB Reports 2017; 50(4): 214-219].
Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea.
Heejin
H
Lee
Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea.
Hyosun
H
Tak
Department of Gerontology, Graduate School of East-West Medical Science, Kyung Hee University, Yongin 17014, Korea.
So Jung
SJ
Park
Department of Gerontology, Graduate School of East-West Medical Science, Kyung Hee University, Yongin 17014, Korea.
Yoon Kyung
YK
Jo
Department of Gerontology, Graduate School of East-West Medical Science, Kyung Hee University, Yongin 17014, Korea.
Dong Hyung
DH
Cho
Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea.
Eun Kyung
EK
Lee
D020413:3' Untranslated Regions; D000255:Adenosine Triphosphate / Q000378:metabolism; D000070416:Antagomirs / Q000378:metabolism; D001483:Base Sequence; D002460:Cell Line; D034281:Dynamins; D020558:GTP Phosphohydrolases / Q000378:metabolism; D006801:Humans; D053078:Membrane Potential, Mitochondrial; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D063154:Mitochondrial Dynamics / Q000502:physiology*; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D010101:Oxygen Consumption; D016415:Sequence Alignment
D020413:3' Untranslated Regions; D000070416:Antagomirs; D008565:Membrane Proteins; C533267:Mff protein, human; D035683:MicroRNAs; D008869:Microtubule-Associated Proteins; D033681:Mitochondrial Membrane Transport Proteins; D024101:Mitochondrial Proteins; D000255:Adenosine Triphosphate; D020558:GTP Phosphohydrolases; C415198:OPA1 protein, human; C470017:Mfn1 protein, human; C110768:DNM1L protein, human; D034281:Dynamins