Growth-inducing effects of argon plasma on soybean sprouts via the regulation of demethylation levels of energy metabolism-related genes.
This study was conducted to determine the effects of argon plasma on the growth of soybean [Glycine max (L.) Merr.] sprouts and investigate the regulation mechanism of energy metabolism. The germination and growth characteristics were modified by argon plasma at different potentials and exposure durations. Upon investigation, plasma treatment at 22.1 kV for 12 s maximized the germination and seedling growth of soybean, increasing the concentrations of soluble protein, antioxidant enzymes, and adenosine triphosphate (ATP) as well as up-regulating ATP a1, ATP a2, ATP b1, ATP b2, ATP b3, target of rapamycin (TOR), growth-regulating factor (GRF) 1-6, down-regulating ATP MI25 mRNA expression, and increasing the demethylation levels of the sequenced region of ATP a1, ATP b1, TOR, GRF 5, and GRF 6 of 6-day-old soybean sprouts. These observations indicate that argon plasma promotes soybean seed germination and sprout growth by regulating the demethylation levels of ATP, TOR, and GRF.
Laboratory of Animal Genetic Engineering and Stem Cell Biology, Department of Animal Biotechnology and Advance Next Generation Convergence Technology, Jeju National University, Jeju 690756, South Korea.
Jiao Jiao
JJ
Zhang
Department of Chemical and Biological Engineering, Jeju National University, Jeju 690756, South Korea.
Jin Oh
JO
Jo
Laboratory of Animal Genetic Engineering and Stem Cell Biology, Department of Animal Biotechnology and Advance Next Generation Convergence Technology, Jeju National University, Jeju 690756, South Korea.
Do Luong
DL
Huynh
Laboratory of Animal Genetic Engineering and Stem Cell Biology, Department of Animal Biotechnology and Advance Next Generation Convergence Technology, Jeju National University, Jeju 690756, South Korea.
Raj Kumar
RK
Mongre
Laboratory of Animal Genetic Engineering and Stem Cell Biology, Department of Animal Biotechnology and Advance Next Generation Convergence Technology, Jeju National University, Jeju 690756, South Korea.
Mrinmoy
M
Ghosh
Laboratory of Animal Genetic Engineering and Stem Cell Biology, Department of Animal Biotechnology and Advance Next Generation Convergence Technology, Jeju National University, Jeju 690756, South Korea.
Amit Kumar
AK
Singh
Department of Chemical and Biological Engineering, Jeju National University, Jeju 690756, South Korea.
Sang Baek
SB
Lee
Department of Chemical and Biological Engineering, Jeju National University, Jeju 690756, South Korea.
Young Sun
YS
Mok
Intellectual Property Law Firm PCR, Seoul 06194, South Korea.
Park
P
Hyuk
Laboratory of Animal Genetic Engineering and Stem Cell Biology, Department of Animal Biotechnology and Advance Next Generation Convergence Technology, Jeju National University, Jeju 690756, South Korea.
Dong Kee
DK
Jeong
D001128:Argon / Q000494:pharmacology*; D000073398:Demethylation; D004734:Energy Metabolism / Q000187:drug effects / Q000235:genetics*; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D018525:Germination; D010940:Plant Proteins / Q000235:genetics*; D058626:Plasma Gases / Q000494:pharmacology*; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D013025:Glycine max / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism
D010940:Plant Proteins; D058626:Plasma Gases; D001128:Argon