In vitro evaluation of wound healing and antimicrobial potential of ozone therapy.
Although ozone therapy is extensively applied when wound repair and antimicrobial effect are necessary, little is known about cellular mechanisms regarding this process. Thus, this study aimed to evaluate ozone cytotoxicity in fibroblasts (L929) and keratinocytes (HaCaT) cell lines, its effects on cell migration and its antimicrobial activity. Cells were treated with ozonated phosphate-buffered saline (8, 4, 2, 1, 0.5 and 0.25 μg/mL ozone), chlorhexidine 0.2% or buffered-solution, and cell viability was determined through MTT assay. The effect of ozone on cell migration was evaluated through scratch wound healing and transwell migration assays. The minimum inhibitory concentrations for Candida albicans and Staphylococcus aureus were determined. Ozone showed no cytotoxicity for the cell lines, while chlorhexidine markedly reduced cell viability. Although no significant difference between control and ozone-treated cells was observed in the scratch assay, a considerable increase in fibroblasts migration was noticed on cells treated with 8 μg/mL ozonated solution. Ozone alone did not inhibit growth of microorganisms; however, its association with chlorhexidine resulted in antimicrobial activity. This study confirms the wound healing and antimicrobial potential of ozone therapy and presents the need for studies to elucidate the molecular mechanisms through which it exerts such biological effects.
Faculty of Health Sciences, University of Brasília, Brasília, Brazil.
Gabriel Álvares
GÁ
Borges
Faculty of Health Sciences, University of Brasília, Brasília, Brazil.
Silvia Taveira
ST
Elias
Faculty of Health Sciences, University of Brasília, Brasília, Brazil.
Sandra Márcia Mazutti
SM
da Silva
Faculty of Health Sciences, University of Brasília, Brasília, Brazil.
Pérola Oliveira
PO
Magalhães
Faculty of Health Sciences, University of Brasília, Brasília, Brazil.
Sergio Bruzadelli
SB
Macedo
Faculty of Health Sciences, University of Brasília, Brasília, Brazil.
Ana Paula Dias
AP
Ribeiro
Faculty of Health Sciences, University of Brasília, Brasília, Brazil. Electronic address: elieteneves@unb.br.
Eliete Neves Silva
EN
Guerra
D000890:Anti-Infective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002176:Candida albicans / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D066298:In Vitro Techniques; D015603:Keratinocytes / Q000166:cytology; D008826:Microbial Sensitivity Tests / Q000379:methods; D010126:Ozone / Q000494:pharmacology* / Q000627:therapeutic use; D013211:Staphylococcus aureus / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects*
D000890:Anti-Infective Agents; D010126:Ozone
Antimicrobial effect; Cell migration; Cytotoxicity; Ozone therapy; Wound healing