Evaluation of Antioxidant, Anti-cholinesterase, and Anti-inflammatory Effects of Culinary MushroomPleurotus pulmonarius.
Culinary mushroomPleurotus pulmonariushas been popular in Asian countries. In this study, the anti-oxidant, cholinesterase, and inflammation inhibitory activities of methanol extract (ME) of fruiting bodies ofP. pulmonariuswere evaluted. The 1,1-diphenyl-2-picryl-hydrazy free radical scavenging activity of ME at 2.0 mg/mL was comparable to that of butylated hydroxytoluene, the standard reference. The ME exhibited significantly higher hydroxyl radical scavenging activity than butylated hydroxytoluene. ME showed slightly lower but moderate inhibitory activity against acetylcholinesterase (AChE) and butyrylcholinesterase than galantamine, a standard AChE inhibitor. It also exhibited protective effect against cytotoxicity to PC-12 cells induced by glutamate (10~100 µg/mL), inhibitory effect on nitric oxide (NO) production and inducible nitric oxide synthase protein expression in lipopolysaccharide-stimulated RAW 264.7 macrophages, and carrageenan-induced paw edema in a rat model. High-performance liquid chromatography analysis revealed the ME ofP. pulmonariuscontained at least 10 phenolic compounds and some of them were identified by the comparison with known standard phenolics. Taken together, our results demonstrate that fruiting bodies ofP. pulmonariuspossess antioxidant, anti-cholinesterase, and inflammation inhibitory activities.
Division of Life Sciences, Incheon National University, Incheon 22012, Korea.
Trung Kien
TK
Nguyen
Division of Life Sciences, Incheon National University, Incheon 22012, Korea.
Kyung Hoan
KH
Im
Division of Life Sciences, Incheon National University, Incheon 22012, Korea.
Jaehyuk
J
Choi
Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, Eumseong 27709, Korea.
Pyung Gyun
PG
Shin
Division of Life Sciences, Incheon National University, Incheon 22012, Korea.
Tae Soo
TS
Lee
Anti-cholinesterase; Anti-inflammation; Antioxidant; Cytotoxicity; Pleurotus pulmonarius