1,25(OH)2 vitamin D3 signalling on immature rat Sertoli cells: gamma-glutamyl transpeptidase and glucose metabolism.
1α,25-Dihydroxyvitamin D3(1,25-D3) is critical for the maintenance of normal male reproduction since reduced fertility is observed in vitamin D-deficient rats. Gamma-glutamyl transpeptidase (GGT) is a membrane-bound enzyme that is localized on Sertoli cells and catalyses the transfer of the gamma-glutamyl residues to an amino acid or peptide acceptor. Sertoli cells are also responsible for providing nutrients, as lactate, to the development of germ cells. The aim of this study was to investigate the effect and the mechanism of action of 1,25-D3on GGT on Sertoli cell functions from 30-day-old immature rat testis. Results demonstrated that 1,25-D3stimulates GGT activity at Sertoli cells plasma membrane through a PKA-dependent mechanism of action, which was not dependent of active de novo protein synthesis. The hormone increases glucose uptake, as well as lactate production and release by Sertoli cells without altering the reactive oxygen species (ROS) generation. In addition, 1,25-D3did not change reduced glutathione (GSH) amount or oxygen consumption, and diminished Sertoli cell death. These findings demonstrate that 1,25-D3stimulatory effect on GGT activity, glucose uptake, LDH activity and lactate production seem to be an important contribution of Sertoli cells for germ cells nutrition and for a full and active ongoing spermatogenesis.
Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário, Bairro Trindade, Cx Postal 5069, Florianópolis, Santa Catarina, CEP: 88040-970, Brazil.
Renata
R
Gonçalves
Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário, Bairro Trindade, Cx Postal 5069, Florianópolis, Santa Catarina, CEP: 88040-970, Brazil.
Ariane
A
Zamoner
Departamento de Enfermagem, Centro de Educação Superior do Oeste, Universidade do Estado de Santa Catarina, Chapecó, Santa Catarina, Brazil.
Leila
L
Zanatta
Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário, Bairro Trindade, Cx Postal 5069, Florianópolis, Santa Catarina, CEP: 88040-970, Brazil.
Ana Paula
AP
Zanatta
Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário, Bairro Trindade, Cx Postal 5069, Florianópolis, Santa Catarina, CEP: 88040-970, Brazil.
Aline Pertile
AP
Remor
Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário, Bairro Trindade, Cx Postal 5069, Florianópolis, Santa Catarina, CEP: 88040-970, Brazil.
Débora
D
da Luz Scheffer
Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário, Bairro Trindade, Cx Postal 5069, Florianópolis, Santa Catarina, CEP: 88040-970, Brazil.
Alexandra
A
Latini
Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário, Bairro Trindade, Cx Postal 5069, Florianópolis, Santa Catarina, CEP: 88040-970, Brazil. mena.barreto@ufsc.br.
Fátima Regina Mena Barreto
FRMB
Silva
Gamma-glutamyl transpeptidase; Glucose; Lactate; Sertoli cells; Testis; Vitamin D3