The diagnostic sensitivity of circulating cardio-enriched microRNAs is increased after normalization of high-density lipoprotein levels.
BACKGROUND
Emerging evidence suggests that microRNAs (miRs) could be a potential biomarker to identify early molecular alterations in the heart. HDL are the major carriers for miRs into the circulation. This study tested whether changes in the level of HDL could affect the diagnostic sensitivity of miRs.


RESULTS
Peripheral blood samples were collected from 20 diabetic and 22 age and gender matched non-diabetic patients undergoing coronary artery bypass graft (CABG) surgery for ischemic heart disease (IHD). Total RNA was extracted from the separated plasma and stored in -80°C. Reverse transcription and amplification using specific primers against cardio-enriched miR-1, -34a, -126, -133, and -499 showed significant correlation between HDL levels and miR-1, -133 and -499. Importantly, normalization of miR levels with HDL showed a significant downregulation of miR-1, -133 and -499 in diabetic plasma, which was not observed before normalization with HDL levels.


CONCLUSIONS
Normalization of circulating miR levels with HDL increases the diagnostic sensitivity of circulating miRs.
Department of Physiology, HeartOtago, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.
Thrishila Parshu
TP
Ram
Department of Physiology, HeartOtago, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.
Ingrid
I
Fomison-Nurse
Department of Physiology, HeartOtago, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.
Sophie
S
Gandhi
Department of Medicine, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.
Sean
S
Coffey
Department of Cardiothoracic Surgery and Surgery, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.
Pankaj
P
Saxena
Department of Cardiothoracic Surgery and Surgery, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.
Ivor
I
Galvin
Department of Cardiothoracic Surgery and Surgery, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.
Richard
R
Bunton
Department of Medicine, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.
Michael J A
MJA
Williams
Department of Physiology, HeartOtago, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.
Regis R
RR
Lamberts
Department of Physiology, HeartOtago, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand. Electronic address: rajesh.katare@otago.ac.nz.
Rajesh
R
Katare
D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D000074166:Circulating MicroRNA / Q000097:blood*; D001026:Coronary Artery Bypass / Q000639:trends; D003324:Coronary Artery Disease / Q000097:blood* / Q000453:epidemiology / Q000601:surgery; D003920:Diabetes Mellitus / Q000097:blood* / Q000453:epidemiology / Q000601:surgery; D005260:Female; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009520:New Zealand / Q000453:epidemiology
D015415:Biomarkers; D000074166:Circulating MicroRNA; D008075:Lipoproteins, HDL
Biomarkers; Circulating miRs; Diabetes; HDL; Ischemic heart disease