Lectin from Sambucus sieboldiana abrogates the anoikis resistance of colon cancer cells conferred by N-acetylglucosaminyltransferase V during hematogenous metastasis.
Anoikis is a form of anchorage-dependent apoptosis, and cancer cells adopt anokis-resistance molecular machinery to conduct metastasis. Here, we report that N-acetylglucosaminyltransferase V gene expression confers anoikis resistance during cancer progression. Overexpression of N-acetylglucosaminyltransferase V protected detached cancer cells from apoptotic death, and suppression or knockout of the gene sensitized cancer cells to the apoptotic death. The gene expression also stimulated anchorage-dependent as well as anchorage-independent colony formation of cancer cells following anoikis stress treatments. Importantly, treatment with the lectin from Sambucus sieboldiana significantly sensitized anoikis-induced cancer cell deaths in vitro as well as in vivo. We propose that the lectin alone or an engineered form could offer a new therapeutic treatment option for cancer patients with advanced tumors.
Genome Editing Research Center, KRIBB, Daejeon, South Korea.
Kyoung Jin
KJ
Song
Genome Editing Research Center, KRIBB, Daejeon, South Korea.|Department of Chemistry, Chungnam National University, Daejeon, South Korea.
Seong Kook
SK
Jeon
Genome Editing Research Center, KRIBB, Daejeon, South Korea.|Department of Biomolecular Science, Korea University of Science and Technology, Daejeon, South Korea.
Su Bin
SB
Moon
Genome Editing Research Center, KRIBB, Daejeon, South Korea.|Department of Biomolecular Science, Korea University of Science and Technology, Daejeon, South Korea.
Jin Suk
JS
Park
Biotherapeutics Translational Research Center, KRIBB, Daejeon, South Korea.|Department of Biomolecular Science, Korea University of Science and Technology, Daejeon, South Korea.
Jang Seong
JS
Kim
Department of Chemistry, Chungnam National University, Daejeon, South Korea.
Jeongkwon
J
Kim
Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, South Korea.|Asia-Pacific Glycomics Reference Site, Daejeon, South Korea.
Sumin
S
Kim
Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, South Korea.|Asia-Pacific Glycomics Reference Site, Daejeon, South Korea.
Hyun Joo
HJ
An
Genome Editing Research Center, KRIBB, Daejeon, South Korea.|Department of Biomolecular Science, Korea University of Science and Technology, Daejeon, South Korea.
Jeong-Heon
JH
Ko
Genome Editing Research Center, KRIBB, Daejeon, South Korea.|Department of Biomolecular Science, Korea University of Science and Technology, Daejeon, South Korea.
Yong-Sam
YS
Kim
D000818:Animals; D023102:Anoikis / Q000187:drug effects* / Q000235:genetics; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D055786:Gene Knockout Techniques; D006801:Humans; D051379:Mice; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics / Q000378:metabolism*; D009362:Neoplasm Metastasis; D037121:Plant Lectins / Q000494:pharmacology*; D027923:Sambucus / Q000737:chemistry*; D059467:Transcriptome; D023041:Xenograft Model Antitumor Assays
D037121:Plant Lectins; D017351:N-Acetylglucosaminyltransferases; C052254:alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase
MGAT5; SSA; anoikis; metastasis