Zwitterionic phosphorylcholine grafted chitosan nanofiber: Preparation, characterization and in-vitro cell adhesion behavior.
In this study, zwitterionic phosphorylcholine grafted electrospun chitosan fiber was accomplished in three steps: (1) Azide groups on the chitosan were regioselectively replaced with hydroxyl side group and then the product was electrospun. (2) Chitosan based macroinitiator was prepared using an azide-alkyne click reaction from azide-functionalized electrospun chitosan fiber. (3) Poly(2-methacryloyloxyethyl phosphorylcholine) (MPC) was grafted onto the electrospun chitosan fiber by atom transfer radical polymerization (ATRP) in order to enhance cellular viability and proliferation of 3T3, ECV and Saos. The structure of surface modified chitosan was characterized by Fourier transform infrared spectrometer (FT-IR) and1H nuclear magnetic resonance (1H NMR). The surface morphology of the nanofibers was investigated by scanning electron microscope (SEM). In-vitro cellular attachment and spreading experiments of 3T3, ECV304 and Saos were performed on electrospun chitosan fibers in the presence and the absence of MPC grafting. Poly(MPC) grafted electrospun fiber showed an excellent performance due to phosphorylcholine groups mimicking the natural phospholipid.
Marmara University, Department of Chemistry, 34722, Goztepe, Istanbul, Turkey.
Burcu
B
Oktay
Marmara University, Department of Chemistry, 34722, Goztepe, Istanbul, Turkey. Electronic address: napohan@marmara.edu.tr.
Nilhan
N
Kayaman-Apohan
Marmara University, Department of Chemistry, 34722, Goztepe, Istanbul, Turkey.
Mediha
M
Süleymanoğlu
Marmara University, Department of Chemistry, 34722, Goztepe, Istanbul, Turkey.
Serap
S
Erdem-Kuruca
D016475:3T3 Cells; D000818:Animals; D001386:Azides / Q000737:chemistry; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry*; D057930:Click Chemistry; D006801:Humans; D051379:Mice; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D017550:Spectroscopy, Fourier Transform Infrared; D054457:Tissue Scaffolds / Q000737:chemistry
D001386:Azides; D011109:Polymethacrylic Acids; C115766:poly(2-methacryloyloxyethyl-phosphorylcholine); D010767:Phosphorylcholine; D048271:Chitosan
Chitosan nanofiber; Click chemistry; Phosphorylcholine; Surface initiated ATRP