Not5-dependent co-translational assembly of Ada2 and Spt20 is essential for functional integrity of SAGA.
Acetylation of histones regulates gene expression in eukaryotes. In the yeast Saccharomyces cerevisiae it depends mainly upon the ADA and SAGA histone acetyltransferase complexes for which Gcn5 is the catalytic subunit. Previous screens have determined that global acetylation is reduced in cells lacking subunits of the Ccr4–Not complex, a global regulator of eukaryotic gene expression. In this study we have characterized the functional connection between the Ccr4–Not complex and SAGA. We show that SAGA mRNAs encoding a core set of SAGA subunits are tethered together for co-translational assembly of the encoded proteins. Ccr4–Not subunits bind SAGA mRNAs and promote the co-translational assembly of these subunits. This is needed for integrity of SAGA. In addition, we determine that a glycolytic enzyme, the glyceraldehyde-3-phosphate dehydrogenase Tdh3, a prototypical moonlighting protein, is tethered at this site of Ccr4–Not-dependent co-translational SAGA assembly and functions as a chaperone.
Department of Microbiology and Molecular Medicine, Faculty of Medicine, Institute of Genetics and Genomics Geneva, University of Geneva, Geneva, Switzerland
Sari
S
Kassem
Department of Microbiology and Molecular Medicine, Faculty of Medicine, Institute of Genetics and Genomics Geneva, University of Geneva, Geneva, Switzerland
Zoltan
Z
Villanyi
Department of Microbiology and Molecular Medicine, Faculty of Medicine, Institute of Genetics and Genomics Geneva, University of Geneva, Geneva, Switzerland
Martine A
MA
Collart
D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D051548:Histone Acetyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D009154:Mutation; D055503:Protein Multimerization / Q000235:genetics; D021122:Protein Subunits; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012260:Ribonucleases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000099224:p300-CBP-Associated Factor
D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating); D051548:Histone Acetyltransferases; D021122:Protein Subunits; D012331:RNA, Fungal; D012333:RNA, Messenger; D012260:Ribonucleases; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C090575:ADA2 protein, S cerevisiae; C111175:NOT5 protein, S cerevisiae; C507785:SAGA complex, S cerevisiae; C099690:SPT20 protein, S cerevisiae; C465226:TDH3 protein, S cerevisiae; C501392:GCN5 protein, S cerevisiae; D000099224:p300-CBP-Associated Factor; C078657:CCR4 protein, S cerevisiae