25-Hydroxyvitamin D-1-α-hydroxylase in apoliporotein E knockout mice: The role of protecting vascular smooth muscle cell from calcification.
Previous publications widely reported that 25-hydroxyvitamin D-1-α-hydroxylase (CYP27B1) regulated the metabolism of 25-hydroxyvitamin D3, which has a close association between altered activity of vitamin D and vascular calcification has been reported in various human diseases, including chronic kidney disease, osteoporosis and atherosclerosis. Vascular calcification is a clinically significant component of atherosclerosis and may be promoted by ROS associated inflammatory. In this study, we evaluated the effect of 25-hydroxyvitamin D-1-α-hydroxylase on the atherosclerosis disease both in apolipoprotein (apo) E-/- mice and wild-type mice. We also isolated endothelial cell (ECs) and vascular smooth muscle cells (VSMCs) in aortic from the wild type mice and apoE-/- mice respectively, then investigated that after parathyroid hormone (PTH) both of the CYP27B1 and vitamin D receptor (VDR) expressions in apoE-/-EC and apoE-/-VSMC were higher than the wide-type EC and VSMCs. However, the increased proliferation and decreased apoptosis have showed in EC and VSMC compared with the cells from apo E-/- mice. Moreover, the index associated with vascular calcification such as intracellular Ca2+concentration and alkaline phosphatase (ALP) activity have been tested and the result suggested that the levels of the former index have improved in the apoE-/-EC and apoE-/-VSMC. We got similar conclusions under the pre-treatment with 1, 25(OH) 2D3.
Department of Pediatrics, Hainan General Hospital, Haikou 570102, China; Department of Pediatrics, Maternal and Child Health care Hospital of Hainan Province, Haikou 570206, China.
Wei
W
Xiang
Department of Cardiology, Hainan General Hospital, Haikou 570102, China.
Wang
W
Liao
Department of Nephropathy, Children's Medical Center, The Second Xiangya Hospital, Central South University, Changsha, 410000, China.
Zhuwen
Z
Yi
Department of Nephropathy, Children's Medical Center, The Second Xiangya Hospital, Central South University, Changsha, 410000, China. Electronic address: xahvvi@sina.com.
Xiaojie
X
He
Department of Dermatology, Maternal and Child Health care Hospital of Hainan Province,15 Long Kun-Nan Road, Haikou 570206, China. Electronic address: yandinghk2016@163.com.
Yan
Y
Ding
D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase / Q000235:genetics / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001057:Apolipoproteins E / Q000172:deficiency* / Q000378:metabolism; D002114:Calcinosis / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D002118:Calcium / Q000378:metabolism; D019610:Cytoprotection; D008297:Male; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009131:Muscle, Smooth, Vascular / Q000473:pathology*; D032389:Myocytes, Smooth Muscle / Q000201:enzymology* / Q000473:pathology*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D018167:Receptors, Calcitriol / Q000235:genetics / Q000378:metabolism
D001057:Apolipoproteins E; D017382:Reactive Oxygen Species; D018167:Receptors, Calcitriol; D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase; D000469:Alkaline Phosphatase; D002118:Calcium
25-Hydroxyvitamin D-1-α-hydroxylase; Apoliporotein E; Vascular smooth muscle cell; Vitamin D receptor