In vitro antimicrobial activity of LED irradiation on Pseudomonas aeruginosa.
Pseudomonas aeruginosa is an opportunistic pathogen responsible of many deaths due to nosocomial pneumonia each year. It is particularly resistant to many different classes of antibiotics and disinfectants. For all these reasons, there is the necessity to find novel approaches of treatment. The aim of this study was to evaluate the effect of 880nm light emitting diodes (LED) irradiation on P. aeruginosa, in vitro. Different LED irradiation parameters (time, energy output and the addition of methylene blue and chlorhexidine) have been tested in order to evaluate the effects on this bacterium. After treatment, the colony forming units per milliliter (CFU mL-1) were recorded and the data were submitted to ANOVA and Bonferroni post hoc tests at a level of significance of 5%. A statistical significant reduction of bacterial count has been registered after 5min of LED irradiation. The antibacterial effect was directly proportional to irradiation time and the output energy. The pre-treatment with methylene blue, seems to be not effective against P. aeruginosa, independently from irradiation parameters. On the contrary, the contemporary action of LED and chlorhexidine has shown a great reduction of bacterial count that was statistical significant respect chlorhexidine and LED alone. The effect of LED irradiation was visible also after 24h, when a lower bacterial count characterized all irradiated samples respect controls.
Dental Materials and Medical Chemistry Unit, Department of Medical, Oral and Biotechnological Sciences, University of Chieti, Via dei Vestini 31, 66013 Italy.
Morena
M
Petrini
Dental Materials and Medical Chemistry Unit, Department of Medical, Oral and Biotechnological Sciences, University of Chieti, Via dei Vestini 31, 66013 Italy.
Paolo
P
Trentini
Paediatric Dentistry Unit, Department of Medical, Oral and Biotechnological Sciences, University of Chieti, Via dei Vestini 31, 66013, Italy.
Domenico
D
Tripodi
Dental Materials and Medical Chemistry Unit, Department of Medical, Oral and Biotechnological Sciences, University of Chieti, Via dei Vestini 31, 66013 Italy. Electronic address: materialidentari.uda@gmail.com.
Giuseppe
G
Spoto
Microbiology and Paediatric Dentistry Unit, Department of Medical, Oral and Biotechnological Sciences, University of Chieti, Via dei Vestini 31, 66013, Italy.
Simonetta
S
D'Ercole
D000890:Anti-Infective Agents; D002710:Chlorhexidine / Q000494:pharmacology; D015169:Colony Count, Microbial; D003131:Combined Modality Therapy; D004202:Disinfectants; D006801:Humans; D007259:Infrared Rays; D054023:Lasers, Semiconductor; D008751:Methylene Blue / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000528:radiation effects*
D000890:Anti-Infective Agents; D004202:Disinfectants; D002710:Chlorhexidine; D008751:Methylene Blue
Bacteria; Chlorhexidine; Led; Light therapy; Periodontitis; Pseudomonas aeruginosa