Production and Partial Characterization ofα-Amylase Enzyme fromBacillussp. BCC 01-50 and Potential Applications.
Amylase is an industrially important enzyme and applied in many industrial processes such as saccharification of starchy materials, food, pharmaceutical, detergent, and textile industries. This research work deals with the optimization of fermentation conditions forα-amylase production from thermophilic bacterial strainBacillussp.BCC 01-50and characterization of crude amylase. The time profile of bacterial growth and amylase production was investigated in synthetic medium and maximum enzyme titer was observed after 60 h. In addition, effects of different carbon sources were tested as a substrate for amylase production and molasses was found to be the best. Various organic and inorganic compounds, potassium nitrate, ammonium chloride, sodium nitrate, urea, yeast extract, tryptone, beef extract, and peptone, were used and beef extract was found to be the best among the nitrogen sources used. Temperature, pH, agitation speed, and size of inoculum were also optimized. Highest enzyme activity was obtained when the strain was cultured in molasses medium for 60 h in shaking incubator (150 rpm) at 50°C and pH 8. Crude amylase showed maximal activity at pH 9 and 65°C. Enzyme remained stable in alkaline pH range 9-10 and 60-70°C. Crude amylase showed great potential for its application in detergent industry and saccharification of starchy materials.
College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
Altaf Ahmed
0000-0001-9455-5207
AA
Simair
Institute of Biotechnology and Genetic Engineering, University of Sindh, Jamshoro 76080, Pakistan.
Abdul Sattar
AS
Qureshi
Institute of Biotechnology and Genetic Engineering, University of Sindh, Jamshoro 76080, Pakistan.
Imrana
I
Khushk
Department of Chemical Engineering, University of Engineering & Technology, KSK Campus, Lahore 54890, Pakistan; State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai 200237, China.
Chaudhry Haider
CH
Ali
Institute of Biotechnology and Genetic Engineering, University of Sindh, Jamshoro 76080, Pakistan.
Safia
S
Lashari
College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China; Institute of Biotechnology and Genetic Engineering, University of Sindh, Jamshoro 76080, Pakistan.
Muhammad Aqeel
MA
Bhutto
Institute of Biotechnology and Genetic Engineering, University of Sindh, Jamshoro 76080, Pakistan.
Ghulam Sughra
GS
Mangrio
College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
Changrui
C
Lu
D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development; D061249:Batch Cell Culture Techniques; D002244:Carbon / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis / Q000187:drug effects; D007700:Kinetics; D008965:Molasses; D009584:Nitrogen / Q000494:pharmacology; D013696:Temperature; D000516:alpha-Amylases / Q000096:biosynthesis*
D003902:Detergents; D002244:Carbon; D000516:alpha-Amylases; D009584:Nitrogen