Hormonal Treatment Effects on the Cross-sectional Area of Pubococcygeus Muscle Fibers After Denervation and Castration in Male Rats.
We explore the interaction of muscle innervation and gonadal hormone action in the pubococcygeus muscle (Pcm) after castration and hormone replacement. Male Wistar rats were castrated and the Pcm was unilaterally denervated; after 2 or 6 weeks, the cross-sectional area (CSA) of Pcm fibers was assessed. Additional groups of castrated rats were used to examine the effects of hormone replacement. At 2 weeks post surgeries, rats were implanted with Silastic capsules containing either dihydrotestosterone (DHT), estradiol benzoate (EB) or both hormones, and the CSA of Pcm fibers was assessed after 4 weeks of hormone treatment. At 2 weeks post surgeries, gonadectomy without hormone replacement resulted in reductions in the CSA of Pcm fibers, and denervation combined with castration increased the magnitude of this effect; further reductions in CSA were present at 6 weeks post surgeries, but again denervation combined with castration increased the magnitude of this effect. Hormone replacement with DHT resulted in hypertrophy in the CSA of nondenervated muscles compared to those of intact normal males, but this effect was attenuated in denervated muscles. Hormone replacement with EB treatment prevented further castration-induced reductions in CSA of nondenervated muscles, but denervation prevented this effect. Similar to that seen with treatment with EB alone, combined treatment with both DHT and EB prevented further reductions in CSA of Pcm fibers in nondenervated muscles, but again denervation attenuated this effect. Thus, while hormone replacement can reverse or prevent further castration-induced atrophy of Pcm fibers, these effects are dependent on muscle innervation. Anat Rec, 300:1327-1335, 2017. © 2017 Wiley Periodicals, Inc.
Departamento de Neurofisiología, Instituto de Neuroetología, Universidad Veracruzana, Xalapa, Veracruz, México.|Departamento de Endocrinología, Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México.
Miguel
M
Lara-García
Departamento de Neurofisiología, Instituto de Neuroetología, Universidad Veracruzana, Xalapa, Veracruz, México.
Mayvi
M
Alvarado
Departamento de Endocrinología, Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México.
Estela
E
Cuevas
Departamento de Neurofisiología, Instituto de Neuroetología, Universidad Veracruzana, Xalapa, Veracruz, México.
Omar
O
Lara-García
Department of Psychological and Brain Sciences, Indiana University, Bloomington, Indiana.
Dale R
DR
Sengelaub
Departamento de Neurofisiología, Instituto de Neuroetología, Universidad Veracruzana, Xalapa, Veracruz, México.|Departamento de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, DF, México.
Pablo
P
Pacheco
D019412:Anatomy, Cross-Sectional; D000818:Animals; D002369:Castration; D012739:Gonadal Steroid Hormones / Q000494:pharmacology*; D008297:Male; D009121:Muscle Denervation; D018485:Muscle Fibers, Skeletal / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar
D012739:Gonadal Steroid Hormones
castration; denervation; gonadal hormones; skeletal muscle