The septin-associated kinase Gin4 recruits Gps1 to the site of cell division.
Cell cycle-dependent morphogenesis of unicellular organisms depends on the spatiotemporal control of cell polarity. Rho GTPases are the major players that guide cells through structural reorganizations such as cytokinesis (Rho1 dependent) and polarity establishment (Cdc42 dependent). In budding yeast, the protein Gps1 plays a pivotal role in both processes. Gps1 resides at the bud neck to maintain Rho1 localization and restrict Cdc42 activity during cytokinesis. Here we analyze how Gps1 is recruited to the bud neck during the cell cycle. We show that different regions of Gps1 and the septin-associated kinase Gin4 are involved in maintaining Gps1 at the bud neck from late G1 phase until midanaphase. From midanaphase, the targeting function of Gin4 is taken over by the bud neck-associated protein Nba1. Our data show that Gps1 is targeted to the cell division site in a biphasic manner, via Gin4 and Nba1, to control the spatiotemporal activation of Rho1 and inhibition of Cdc42.
Centre for Organismal Studies and German Cancer Research Center, DKFZ-ZMBH Alliance, and Molecular Biology of Centrosomes and Cilia Unit, University of Heidelberg, 69120 Heidelberg, Germany.
Franz
F
Meitinger
Centre for Organismal Studies and German Cancer Research Center, DKFZ-ZMBH Alliance, and Molecular Biology of Centrosomes and Cilia Unit, University of Heidelberg, 69120 Heidelberg, Germany gislene.pereira@cos.uni-heidelberg.de.
Gislene
G
Pereira
D002453:Cell Cycle / Q000502:physiology; D018797:Cell Cycle Proteins / Q000378:metabolism; D002455:Cell Division / Q000502:physiology; D018844:Cyclin-Dependent Kinases / Q000378:metabolism*; D048749:Cytokinesis; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008938:Mitosis; D011494:Protein Kinases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D058112:Septins / Q000378:metabolism; D015398:Signal Transduction; D020741:rho GTP-Binding Proteins / Q000378:metabolism
C000592814:Aim44 protein, S cerevisiae; D018797:Cell Cycle Proteins; D047908:Intracellular Signaling Peptides and Proteins; C000596183:Nba1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D011494:Protein Kinases; C107283:GIN4 protein, S cerevisiae; D018844:Cyclin-Dependent Kinases; D058112:Septins; D020741:rho GTP-Binding Proteins