Transcriptional regulation of male-sterility in 7B-1 male-sterile tomato mutant.
The 7B-1 tomato (Solanum lycopersicum L. cv Rutgers) is a male-sterile mutant with enhanced tolerance to abiotic stress, which makes it a potential candidate for hybrid seed breeding and stress engineering. To underline the molecular mechanism regulating the male-sterility in 7B-1, transcriptomic profiles of the 7B-1 male-sterile and wild type (WT) anthers were studied using mRNA sequencing (RNA-Seq). In total, 768 differentially expressed genes (DEGs) were identified, including 132 up-regulated and 636 down-regulated transcripts. Gene ontology (GO) enrichment analysis of DEGs suggested a general impact of the 7B-1 mutation on metabolic processes, such as proteolysis and carbohydrate catabolic process. Sixteen candidates with key roles in regulation of anther development were subjected to further analysis using qRT-PCR and in situ hybridization. Cytological studies showed several defects associated with anther development in the 7B-1 mutant, including unsynchronized anther maturation, dysfunctional meiosis, arrested microspores, defect in callose degradation and abnormal tapetum development. TUNEL assay showed a defect in programmed cell death (PCD) of tapetal cells in 7B-1 anthers. The present study provides insights into the transcriptome of the 7B-1 mutant. We identified several genes with altered expression level in 7B-1 (including beta-1,3 glucanase, GA2oxs, cystatin, cysteine protease, pectinesterase, TA29, and actin) that could potentially regulate anther developmental processes, such as meiosis, tapetum development, and cell-wall formation/degradation.
Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University and Institute of Experimental Botany AS CR, Šlechtitelů 27, Olomouc-Holice, Czech Republic.
Vahid
V
Omidvar
School of Computing Sciences, University of East Anglia, Norwich, United Kingdom.|School of Biological Sciences, University of East Anglia, Norwich, United Kingdom.
Irina
I
Mohorianu
School of Biological Sciences, University of East Anglia, Norwich, United Kingdom.
Tamas
T
Dalmay
Boyce Thompson Institute, Cornell University, Ithaca, NY, United States of America.
Yi
Y
Zheng
Boyce Thompson Institute, Cornell University, Ithaca, NY, United States of America.
Zhangjun
Z
Fei
Department of Agricultural and Forestry Sciences, University of Tuscia, Viterbo, Italy.
Anna
A
Pucci
Department of Agricultural and Forestry Sciences, University of Tuscia, Viterbo, Italy.
Andrea
A
Mazzucato
Department of Botany, Faculty of Science, Palacký University in Olomouc, Šlechtitelů 27, Olomouc-Holice, Czech Republic.
Vendula
V
Večeřová
Department of Botany, Faculty of Science, Palacký University in Olomouc, Šlechtitelů 27, Olomouc-Holice, Czech Republic.
Michaela
M
Sedlářova
Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University and Institute of Experimental Botany AS CR, Šlechtitelů 27, Olomouc-Holice, Czech Republic.
Martin
M
Fellner
D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D005936:Glucans / Q000378:metabolism; D018551:Solanum lycopersicum / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D051479:Plant Infertility / Q000235:genetics*; D014158:Transcription, Genetic
D005936:Glucans; C048306:callose