Magnetic nanoparticle-loaded electrospun polymeric nanofibers for tissue engineering.
Magnetic nanoparticles have been one of the most attractive nanomaterials for various biomedical applications including magnetic resonance imaging (MRI), diagnostic contrast enhancement, magnetic cell separation, and targeted drug delivery. Three-dimensional (3-D) fibrous scaffolds have broad application prospects in the biomedical field, such as drug delivery and tissue engineering. In this work, a novel three-dimensional composite membrane composed of the tri-block copolymer poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-PEG-PCL, PCEC) and magnetic iron oxide nanoparticles (Fe3O4NPs) were fabricated using electrospinning technology. The physico-chemical properties of the PCEC/Fe3O4membranes were investigated by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and differential scanning calorimetry (DSC). Morphological observation using scanning electron microscopy (SEM) showed that the composite fibers containing 5% Fe3O4nanoparticles had a diameter of 250nm. In vitro cell culture of NIH 3T3 cells on the PCEC/Fe3O4membranes showed that the PCEC/Fe3O4fibers might be a suitable scaffold for cell adhesion. Moreover, MTT analysis also demonstrated that the membranes possessed lower cytotoxicity. Therefore, this study revealed that the magnetic PCEC/Fe3O4fibers might have great potential for using in skin tissue engineering.
Department of Oncology, The Affiliated Hospital of Southwest Medical University, Southwest Medical University, Luzhou 646000, PR China.
Heng
H
Zhang
Department of Science and Technology, Southwest Medical University, Luzhou 646000, PR China.
JiYi
J
Xia
Health Management Center, The Affiliated Hospital (TCM) of Southwest Medical University, Luzhou 646000, PR China.
XianLun
X
Pang
Department of Oncology, The Affiliated Hospital of Southwest Medical University, Southwest Medical University, Luzhou 646000, PR China.
Ming
M
Zhao
Department of Oncology, The Affiliated Hospital of Southwest Medical University, Southwest Medical University, Luzhou 646000, PR China.
BiQiong
B
Wang
Department of Oncology, The Affiliated Hospital of Southwest Medical University, Southwest Medical University, Luzhou 646000, PR China.
LingLin
L
Yang
Experiment Center of Basic Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, PR China.
HaiSu
H
Wan
Department of Oncology, The Affiliated Hospital of Southwest Medical University, Southwest Medical University, Luzhou 646000, PR China. Electronic address: wjb6147@163.com.
JingBo
J
Wu
Department of Oncology, The Affiliated Hospital of Southwest Medical University, Southwest Medical University, Luzhou 646000, PR China. Electronic address: shaozhifu513@163.com.
ShaoZhi
S
Fu
D000818:Animals; D002152:Calorimetry, Differential Scanning; D002470:Cell Survival; D006868:Hydrolysis; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D008856:Microscopy, Fluorescence; D041681:NIH 3T3 Cells; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011091:Polyesters / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D023822:Tissue Engineering / Q000379:methods*; D014961:X-Ray Diffraction
D058185:Magnetite Nanoparticles; D011091:Polyesters; D011108:Polymers; C515340:poly(epsilon-caprolactone)-b-poly(ethyleneglycol)-b-poly(epsilon-caprolactone); D011092:Polyethylene Glycols
Composite fibers; Electrospinning; Magentic nanoparticles; Poly(ε-caprolactone)-poly(ethylene glycol) copolymer; Tissue engineering