Synthesis and characterization of hollow porous molecular imprinted polymers for the selective extraction and determination of caffeic acid in fruit samples.
Hollow porous molecular imprinted polymers (HPMIPs) were prepared using caffeic acid (CA) as template, 4-vinylpyridine (4-VP) as functional monomer, and MCM-48 as sacrificial support. Large surface area (562.7m2/g) of HPMIPs resulted in high binding capacity (27.26mg/g) and fast kinetic binding (40min) in comparison with solid MIPs. Equilibrium data fitted well to Freundlich equation, and adsorption process with multi-diffusion mechanisms could be described by pseudo-second order model. Selectivity of HPMIPs was favorable. Finally, HPMIPs were successfully used to rapidly and selectively extract CA from fruits with a relatively satisfactory recovery (87.1-101.3%). Coupling with HPLC, contents of CA in kiwifruit, apple, papaya and waxberry were less than 1.0μg/g fresh fruit. Results indicated the superiority of HPMIPs in the separation field.
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
Dengxin
D
Fan
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
Ling
L
Jia
School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, PR China. Electronic address: xhy330@126.com.
Haiyan
H
Xiang
Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, Jishou University, Zhangjiajie 427000, PR China.
Mijun
M
Peng
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
Hui
H
Li
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China; Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, Jishou University, Zhangjiajie 427000, PR China. Electronic address: shuyshi@gmail.com.
Shuyun
S
Shi
D000327:Adsorption; D002109:Caffeic Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005638:Fruit / Q000737:chemistry*; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D052616:Solid Phase Extraction / Q000379:methods*
D002109:Caffeic Acids; D011108:Polymers; C040048:caffeic acid
Caffeic acid; Food analysis; Kinetic models; Molecularly imprinted polymers; Selective extraction