Green synthesis of rifampicin-loaded copper nanoparticles with enhanced antimicrobial activity.
The antimicrobial properties of copper and rifampicin-loaded copper nanoparticles were investigated using four strains: Staphylococcus aureus, Escherichia coli, Bacillus pumilis and Pseudomonas fluorescens. Spherical-shaped copper nanoparticles were synthesized via green reduction method from the peppermint extract. It was found that adsorption of rifampicin on the copper nanosurface enhances its biological activity and prevents the development of resistance. The interactions between rifampicin-copper nanoparticles and bacteria cells were monitored using atomic force microscopy (AFM) and confocal laser scanning microscopy (CLSM). It was proven that loaded with rifampicin copper nanoparticles were able to damage the S. aureus cell membrane and facilitate the bacteria biofilm matrix disintegration. Moreover, the DNA decomposition of S. aureus treated with copper and rifampicin-copper nanoparticles was confirmed by agarose gel electrophoresis. The results obtained indicate that adsorption of rifampicin on the copper nanoparticles surface might provide the reduction of antibiotic dosage and prevent its adverse side effects.
NanoBiomedical Centre, Adam Mickiewicz University in Poznań, Umultowska 85, Poznań, Poland. marta.budych@amu.edu.pl.
Marta J
MJ
Woźniak-Budych
NanoBiomedical Centre, Adam Mickiewicz University in Poznań, Umultowska 85, Poznań, Poland.
Łucja
Ł
Przysiecka
NanoBiomedical Centre, Adam Mickiewicz University in Poznań, Umultowska 85, Poznań, Poland.
Krzysztof
K
Langer
NanoBiomedical Centre, Adam Mickiewicz University in Poznań, Umultowska 85, Poznań, Poland.
Barbara
B
Peplińska
NanoBiomedical Centre, Adam Mickiewicz University in Poznań, Umultowska 85, Poznań, Poland.
Marcin
M
Jarek
NanoBiomedical Centre, Adam Mickiewicz University in Poznań, Umultowska 85, Poznań, Poland.
Maciej
M
Wiesner
NanoBiomedical Centre, Adam Mickiewicz University in Poznań, Umultowska 85, Poznań, Poland.
Grzegorz
G
Nowaczyk
NanoBiomedical Centre, Adam Mickiewicz University in Poznań, Umultowska 85, Poznań, Poland.
Stefan
S
Jurga
D000900:Anti-Bacterial Agents / Q000737:chemistry; D000890:Anti-Infective Agents / Q000737:chemistry*; D001407:Bacillus / Q000187:drug effects; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000032:analysis / Q000737:chemistry; D004058:Diffusion; D015195:Drug Design; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000187:drug effects; D055772:Green Chemistry Technology / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D036103:Nanotechnology / Q000379:methods; D010316:Particle Size; D011551:Pseudomonas fluorescens / Q000187:drug effects; D012293:Rifampin / Q000138:chemical synthesis / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects; D013499:Surface Properties; D013816:Thermodynamics
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D003300:Copper; D004247:DNA; D012293:Rifampin