Overexpression of SKP2 Inhibits the Radiation-Induced Bystander Effects of Esophageal Carcinoma.
To investigate the effects of S-phase kinase protein 2 (SKP2) expression on the radiation induced bystander effect (RIBE) in esophageal cancer (EC) cells.



Western blot was used to detect the levels of SKP2, Rad51, and Ku70 in EC cells. Positive transfection, RNAi, micronucleus (MN), and γ-H2AX focus formation assay were used to investigate the effects of SKP2 on RIBE induced by irradiated cells.



We found a significant negative correlation between SKP2 expression and MN frequency (p< 0.05) induced by RIBE. The results were further confirmed by positive transfection, RNAi, and rescue experiments.γ-H2AX focus formation assay results indicated that overexpression of SKP2 in the irradiated cells inhibited the DNA damage of RIBE cells. However, when SKP2 expression decreased in irradiated cells, the DNA damage of RIBE cells increased. Increased or decreased expression levels of SKP2 had effects on Rad51 expression under the conditions of RIBE.



These results showed, for the first time, that SKP2 expression can inhibit RIBE of EC cells. The mechanism may function, at least partly, through the regulation of Rad51 in the ability to repair DNA damage.
The Beijing Prevention and Treatment Hospital of Occupational Disease for Chemical Industry (Beijing Institute of Occupational Disease Prevention and Treatment), Beijing 100093, China. wxc3188@126.com.
Xiao-Chun
XC
Wang
Department of Nuclear Medicine, The First Affiliated Hospital of Baotou Medical College, Baotou 014010, China. zhangtiejun011@126.com.
Tie-Jun
TJ
Zhang
The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, China. guozijian1101@163.com.
Zi-Jian
ZJ
Guo
The Beijing Prevention and Treatment Hospital of Occupational Disease for Chemical Industry (Beijing Institute of Occupational Disease Prevention and Treatment), Beijing 100093, China. xiaochangyan11@163.com.
Chang-Yan
CY
Xiao
The Beijing Prevention and Treatment Hospital of Occupational Disease for Chemical Industry (Beijing Institute of Occupational Disease Prevention and Treatment), Beijing 100093, China. dingxiaowen00@sohu.com.
Xiao-Wen
XW
Ding
The Beijing Prevention and Treatment Hospital of Occupational Disease for Chemical Industry (Beijing Institute of Occupational Disease Prevention and Treatment), Beijing 100093, China. fangfang001230@126.com.
Fang
F
Fang
The Beijing Prevention and Treatment Hospital of Occupational Disease for Chemical Industry (Beijing Institute of Occupational Disease Prevention and Treatment), Beijing 100093, China. shengwentao112@126.com.
Wen-Tao
WT
Sheng
National Institute for Radiological Protection, China CDC, Beijing 100088, China. shuxu1201@126.com.
Xu
X
Shu
The Beijing Prevention and Treatment Hospital of Occupational Disease for Chemical Industry (Beijing Institute of Occupational Disease Prevention and Treatment), Beijing 100093, China. Lijue101@126.com.
Jue
J
Li
D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D024201:Bystander Effect / Q000502:physiology*; D045744:Cell Line, Tumor; D004249:DNA Damage; D004938:Esophageal Neoplasms / Q000378:metabolism / Q000532:radiotherapy*; D006801:Humans; D048629:Micronuclei, Chromosome-Defective; D011832:Radiation Injuries / Q000235:genetics / Q000378:metabolism*; D044786:S-Phase Kinase-Associated Proteins / Q000378:metabolism*
D014408:Biomarkers, Tumor; D044786:S-Phase Kinase-Associated Proteins
SKP2; esophageal carcinoma; radiation-induced bystander effect