Rapid extraction of DNA from archival clinical specimens: our experiences.
The analysis of DNA extracted from archival clinical specimens using polymerase chain reaction represents the basis of a variety of research and diagnostic protocols in medicine. However, the selection of optimal DNA extraction method is critical if such an analysis is to be successful. Recently, we have evaluated a number of rapid DNA extraction protocols in order to find the most suitable method for routine processing of the most common archival materials in pathological and cytological laboratories: paraffin-embedded tissues and Papanicolaou- or Giemsa-stained smears. Our results demonstrate that rapid DNA extraction methods have comparable DNA extraction efficiencies with standard DNA isolation protocols on archival clinical specimens with the exception of Giemsa-stained smears.
Institute of Microbiology and Immunology, Medical Faculty, Zaloška 4, SI-1105 Ljubljana, Slovenia, , , , , , SI.
Mario
M
Poljak
Institute of Microbiology and Immunology, Medical Faculty, Zaloška 4, SI-1105 Ljubljana, Slovenia, , , , , , SI.
Katja
K
Seme
Institute of Pathology, Medical Faculty, Korytkova 2, SI-1105 Ljubljana, Slovenia, , , , , , SI.
Nina
N
Gale
DNA; Giemsa smears; Key words PCR; Papanicolaou smears; archival specimens