Ultrasensitive Photoelectrochemical Biosensing of Cell Surface N-Glycan Expression Based on the Enhancement of Nanogold-Assembled Mesoporous Silica Amplified by Graphene Quantum Dots and Hybridization Chain Reaction.
An ultrasensitive photoelectrochemical (PEC) biosensor for N-glycan expression based on the enhancement of nanogold-assembled mesoporous silica nanoparticles (GMSNs) was fabricated, which also combined with multibranched hybridization chain reaction (mHCR) and graphene quantum dots (GQDs). In this work, the localized surface plasmon resonance, mHCR and GQDs-induced signal amplification strategies were integrated exquisitely and applied sufficiently. In the fabrication, after porous ZnO spheres immobilized on the Au nanorod-modified paper working electrode were sensitized by CdTe QDs, the GMSNs were assembled on the CdTe QDs. Then the photocurrent efficiency was improved by the sensitization of the CdTe QDs and the localized surface plasmon resonance of GMSNs. Successively, the products of mHCR with multiple biotins for multiple horseradish peroxidase binding and multiple branched arms for capturing the target cells were attached on the as-prepared electrode. The chemiluminescent (CL) emission with the aid of horseradish peroxidase served as an inner light source to excite photoactive materials for simplifying the instrument. Furthermore, the aptamer could capture the cancer cells by its highly efficient cell recognition ability, which avoided the conventional routing cell counting procedures. Meanwhile, the GQDs served as the signal amplication strategy, which was exerted in the process of N-glycan evaluation because the competitive absorption of exciting light and consumption of H2O2served as the electron donor of the PEC system and the oxidant of the luminol-based CL system. This judiciously engineered biosensor offered a promising platform for the exploration of N-glycan-based physiological processes.
School of Chemistry and Chemical Engineering and §School of Biological Science and Technology, University of Jinan , Jinan 250022, P.R. China.
Shenguang
http://orcid.org/0000-0002-0537-6491
S
Ge
School of Chemistry and Chemical Engineering and §School of Biological Science and Technology, University of Jinan , Jinan 250022, P.R. China.
Feifei
F
Lan
School of Chemistry and Chemical Engineering and §School of Biological Science and Technology, University of Jinan , Jinan 250022, P.R. China.
Linlin
L
Liang
School of Chemistry and Chemical Engineering and §School of Biological Science and Technology, University of Jinan , Jinan 250022, P.R. China.
Na
N
Ren
School of Chemistry and Chemical Engineering and §School of Biological Science and Technology, University of Jinan , Jinan 250022, P.R. China.
Li
L
Li
School of Chemistry and Chemical Engineering and §School of Biological Science and Technology, University of Jinan , Jinan 250022, P.R. China.
Haiyun
H
Liu
School of Chemistry and Chemical Engineering and §School of Biological Science and Technology, University of Jinan , Jinan 250022, P.R. China.
Mei
M
Yan
School of Chemistry and Chemical Engineering and §School of Biological Science and Technology, University of Jinan , Jinan 250022, P.R. China.
Jinghua
J
Yu
D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D006108:Graphite; D006861:Hydrogen Peroxide; D011134:Polysaccharides; D016062:Porosity; D045663:Quantum Dots; D012822:Silicon Dioxide
D011134:Polysaccharides; D012822:Silicon Dioxide; D006108:Graphite; D006861:Hydrogen Peroxide
N-glycan evaluation; localized surface plasmon resonance; microfluidic paper-based analytical device; multibranched hybridization chain reaction; photoelectrochemical