Investigating Viruses during the Transformation of Molecular Biology.
This Reflections article describes my early work on viral enzymes and the discovery of mRNA capping, how my training in medicine and biochemistry merged as I evolved into a virologist, the development of viruses as vaccine vectors, and how scientific and technological developments during the 1970s and beyond set the stage for the interrogation of nearly every step in the reproductive cycle of vaccinia virus (VACV), a large DNA virus with about 200 genes. The reader may view this article as a work in progress, because I remain actively engaged in research at the National Institutes of Health (NIH) notwithstanding 50 memorable years there.
From the Laboratory of Viral Diseases, NIAID, National Institutes of Health, Bethesda, Maryland 20892 bmoss@niaid.nih.gov.
Bernard
0000-0002-2154-8564
B
Moss
D006801:Humans; D008967:Molecular Biology; D014615:Vaccinia / Q000235:genetics / Q000517:prevention & control* / Q000821:virology*; D014616:Vaccinia virus / Q000502:physiology*; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication
D014764:Viral Proteins
DNA viruses; mRNA; mRNA capping; molecular biology; vaccine development; vaccine vectors; vaccinia virus; viral replication