Metabolomics analysis of serum from subjects after occupational exposure to acrylamide using UPLC-MS.
Since occupational exposure to acrylamide (ACR) may cause nerve damage, sensitive biomarkers to evaluate the early effects of ACR on human health are needed. In the present study, we have compared a group of individuals with occupational exposure to ACR (contact group, n = 65) with a group of individuals with no exposure (non-contact group, n = 60). Serum metabolomics analysis of the contact and non-contact groups was carried out using ultra performance liquid chromatograph/time of flight mass spectrometry, combined with multivariate analysis, to identify potential metabolites. Serum biochemical indexes of the contact and non-contact groups were also determined using an automatic biochemistry analyzer. There was a clear separation between the contact group and the non-contact group; receiver operator characteristic curve analysis suggested that phytosphingosine, 4E,15Z-bilirubin IXa and tryptophan were the best metabolites to use as biomarkers. Liver function was also found to be abnormal in the contact group. Important, ACR-related, metabolic changes were seen in the contact group and new biomarkers for assessing the toxicity of ACR on the central nervous system have been proposed. This study will provide a sound basis for exploring the toxic mechanisms and metabolic pathways of ACR.
Department of Occupational Health, Public Health College, Harbin Medical University, Harbin, People's Republic of China.
Sheng-Yuan
SY
Wang
Department of Occupational Health, Public Health College, Harbin Medical University, Harbin, People's Republic of China.
Cui-Ping
CP
Yu
Department of Occupational Health, Public Health College, Harbin Medical University, Harbin, People's Republic of China.
Yu-Lin
YL
Pan
Department of Occupational Health, Public Health College, Harbin Medical University, Harbin, People's Republic of China.
Xiao-Rong
XR
Zhou
Department of Occupational Health, Public Health College, Harbin Medical University, Harbin, People's Republic of China.
Rui
R
Xin
Department of Occupational Health, Public Health College, Harbin Medical University, Harbin, People's Republic of China.
Yue
Y
Wang
Harbin Railway Center for Disease Control and Prevention, Harbin, People's Republic of China.
Wei-Wei
WW
Ma
Department of Occupational Health, Public Health College, Harbin Medical University, Harbin, People's Republic of China.
Ran
R
Gao
Heilongjiang Province Safety Production Supervision and Administration Bureau, People's Republic of China.
Chao
C
Wang
Department of Occupational Health, Public Health College, Harbin Medical University, Harbin, People's Republic of China. Electronic address: wuyonghui777@163.com.
Yong-Hui
YH
Wu
D020106:Acrylamide / Q000097:blood*; D000328:Adult; D015415:Biomarkers / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003710:Demography; D016002:Discriminant Analysis; D005260:Female; D006801:Humans; D016018:Least-Squares Analysis; D008297:Male; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D016273:Occupational Exposure / Q000009:adverse effects*; D025341:Principal Component Analysis; D012372:ROC Curve; D044967:Serum / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D015415:Biomarkers; D020106:Acrylamide
Acrylamide; Biomarkers; Central nervous system; Metabolomics; Multivariate analysis; UPLC-QTOF-MS