An air-liquid interphase approach for modeling the early embryo-maternal contact zone.
We developed an air-liquid interphase culture procedure for mammalian oviduct epithelial cells leading to the formation of functional epithelial tissues, which generate oviduct fluid surrogates. These in vitro oviduct epithelia can be co-cultured with living zygotes and enable embryonic development up to the blastocyst stage without addition of embryo culture medium. The described strategy is broadly applicable to analyze early embryo-maternal interactions under standardized in vitro conditions.
Leibniz Institute for Farm Animal Biology, FBN Dummerstorf, Germany.
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Chen
Leibniz Institute for Farm Animal Biology, FBN Dummerstorf, Germany.|Institute of Veterinary Biochemistry, Freie Universitaet Berlin, Germany.
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Palma-Vera
Leibniz Institute for Farm Animal Biology, FBN Dummerstorf, Germany.
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Langhammer
Leibniz Institute for Farm Animal Biology, FBN Dummerstorf, Germany.
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Galuska
Leibniz Institute for Zoo and Wildlife Research, IZW Berlin, Germany.
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Braun
Leibniz Institute for Molecular Pharmacology, FMP Berlin, Germany.
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Krause
Institute of Farm Animal Genetics, Federal Research Institute for Animal Health, Mariensee, Germany.
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Lucas-Hahn
Leibniz Institute for Farm Animal Biology, FBN Dummerstorf, Germany.
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Schoen
D000388:Air; D000818:Animals; D002417:Cattle; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D004622:Embryo, Mammalian / Q000502:physiology*; D004847:Epithelial Cells / Q000166:cytology; D005187:Fallopian Tubes / Q000166:cytology; D005260:Female; D008431:Maternal-Fetal Exchange / Q000502:physiology*; D051379:Mice; D008954:Models, Biological; D011247:Pregnancy; D034421:Sus scrofa; D015053:Zygote / Q000166:cytology