Measurement of Lactate Content and Amide Proton Transfer Values in the Basal Ganglia of a Neonatal Piglet Hypoxic-Ischemic Brain Injury Model Using MRI.
As amide proton transfer imaging is sensitive to protein content and intracellular pH, it has been widely used in the nervous system, including brain tumors and stroke. This work aimed to measure the lactate content and amide proton transfer values in the basal ganglia of a neonatal piglet hypoxic-ischemic brain injury model by using MR spectroscopy and amide proton transfer imaging.



From 58 healthy neonatal piglets (3-5 days after birth; weight, 1-1.5 kg) selected initially, 9 piglets remained in the control group and 43 piglets, in the hypoxic-ischemic brain injury group. Single-section amide proton transfer imaging was performed at the coronal level of the basal ganglia. Amide proton transfer values of the bilateral basal ganglia were measured in all piglets. The ROI of MR spectroscopy imaging was the right basal ganglia, and the postprocessing was completed with LCModel software.



After hypoxic-ischemic insult, the amide proton transfer values immediately decreased, and at 0-2 hours, they remained at their lowest level. Thereafter, they gradually increased and finally exceeded those of the control group at 48-72 hours. After hypoxic-ischemic insult, the lactate content increased immediately, was maximal at 2-6 hours, and then gradually decreased to the level of the control group. The amide proton transfer values were negatively correlated with lactate content (r= -0.79,P< .05).



This observation suggests that after hypoxic-ischemic insult, the recovery of pH was faster than that of lactate homeostasis.
From the Department of Radiology, Shengjing Hospital of China Medical University, Shenyang, PR China.
Y
0000-0002-7452-0725
Y
Zheng
From the Department of Radiology, Shengjing Hospital of China Medical University, Shenyang, PR China. wangxm024@163.com.
X-M
0000-0003-0276-2466
XM
Wang
D000577:Amides / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D001479:Basal Ganglia / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D006863:Hydrogen-Ion Concentration; D020925:Hypoxia-Ischemia, Brain / Q000000981:diagnostic imaging* / Q000378:metabolism*; D019344:Lactic Acid / Q000032:analysis / Q000378:metabolism*; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D011522:Protons; D013552:Swine
D000577:Amides; D011522:Protons; D019344:Lactic Acid