Tomato photorespiratory glycolate-oxidase-derived H2O2production contributes to basal defence against Pseudomonas syringae.
Despite being essential for C3plants, photorespiration is believed to cause a significant crop yield loss even under future climates. However, how photorespiration affects plant basal defence still remains largely unknown. Here, we studied the involvement of photorespiration in tomato-Pseudomonas syringae pv. tomato DC3000 interaction focusing on three photorespiratory genes. Inoculation with P. syringae increased photorespiration rate (Pr) and expression of glycolate oxidase (GOX2), serine glyoxylate aminotransferase (SGT) and serine hydroxyl methyltransferase (SHMT1); however, inhibition of photorespiration by isonicotinic acid hydrazide decreased tomato basal defence against P. syringae. Furthermore, silencing of GOX2, SGT or SHMT1 genes in tomato decreased Pr but increased susceptibility to P. syringae, whereas transient overexpression of GOX2, SGT or SHMT1 in tobacco increased basal defence. Further study revealed that salicylic acid (SA) signalling is involved in GOX2-mediated, SGT-mediated and SHMT1-mediated defence. Moreover, H2O2pretreatment remarkably alleviated the GOX2 silencing-induced depression in basal defence and SA signalling, whereas it had no effect on that of SGT-silenced and SHMT1-silenced plants. Taken together, these results suggest that H2O2is critical for GOX2-modulated but not SGT-modulated or SHMT1-modulated SA signalling and subsequent basal defence against P. syringae. This work deepens the understanding of photorespiration-involved defence responses to bacterial attack in plants.
Department of Horticulture, Zijingang Campus, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.|Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, 866 Yuhangtang Road, Hangzhou, 310058, China.
Golam Jalal
GJ
Ahammed
Department of Horticulture, Zijingang Campus, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.|Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310008, China.
Xin
X
Li
Department of Horticulture, Zijingang Campus, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.
Guanqun
G
Zhang
Department of Horticulture, Zijingang Campus, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.
Huan
H
Zhang
Department of Horticulture, Zijingang Campus, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.
Junying
0000-0001-5351-1910
J
Shi
Department of Horticulture, Zijingang Campus, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.
Caizhe
C
Pan
Department of Horticulture, Zijingang Campus, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.|Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, 866 Yuhangtang Road, Hangzhou, 310058, China.
Jingquan
J
Yu
Department of Horticulture, Zijingang Campus, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.|Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, 866 Yuhangtang Road, Hangzhou, 310058, China.
Kai
K
Shi
D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D012696:Glycine Hydroxymethyltransferase / Q000235:genetics / Q000378:metabolism; D006016:Glycolates / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism*; D018551:Solanum lycopersicum / Q000201:enzymology / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D057865:Plant Immunity; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000276:immunology / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D044224:Pseudomonas syringae / Q000502:physiology*; D020156:Salicylic Acid / Q000378:metabolism; D015398:Signal Transduction; D000637:Transaminases / Q000235:genetics / Q000378:metabolism
D006016:Glycolates; D010940:Plant Proteins; D006861:Hydrogen Peroxide; D000429:Alcohol Oxidoreductases; C020821:glycollate oxidase; D012696:Glycine Hydroxymethyltransferase; D000637:Transaminases; C023067:glyoxylate aminotransferase; D020156:Salicylic Acid
Pseudomonas syringae; Solanum lycopersicum; basal defence; glycolate oxidase (GOX); hydrogen peroxide; photorespiration; salicylic acid; serine glyoxylate aminotransferase (SGT); serine hydroxyl methyltransferase (SHMT)