Vitamin D induces myogenic differentiation in skeletal muscle derived stem cells.
Skeletal muscle wasting is a serious disorder associated with health conditions such as aging, chronic kidney disease and AIDS. Vitamin D is most widely recognized for its regulation of calcium and phosphate homeostasis in relation to bone development and maintenance. Recently, vitamin D supplementation has been shown to improve muscle performance and reduce the risk of falls in vitamin D deficient older adults. However, little is known of the underlying molecular mechanism(s) or the role it plays in myogenic differentiation. We examined the effect of 1,25-D3on myogenic cell differentiation in skeletal muscle derived stem cells. Primary cultures of skeletal muscle satellite cells were isolated from the tibialis anterior, soleus and gastrocnemius muscles of 8-week-old C57/BL6 male mice and then treated with 1,25-D3The efficiency of satellite cells isolation determined by PAX7+ cells was 81%, and they expressed VDR. Incubation of satellite cells with 1,25-D3induces increased expression of: (i) MYOD, (ii) MYOG, (iii) MYC2, (iv) skeletal muscle fast troponin I and T, (v) MYH1, (vi) IGF1 and 2, (vii) FGF1 and 2, (viii) BMP4, (ix) MMP9 and (x) FST. It also promotes myotube formation and decreases the expression of MSTN. In conclusion, 1,25-D3promoted a robust myogenic effect on satellite cells responsible for the regeneration of muscle after injury or muscle waste. This study provides a mechanistic justification for vitamin D supplementation in conditions characterized by loss of muscle mass and also in vitamin D deficient older adults with reduced muscle mass and strength, and increased risk of falls.
Department of Internal MedicineCharles R. Drew University of Medicine and Science, Los Angeles, California, USA.
Melissa
M
Braga
Department of Health & Life SciencesCharles R. Drew University of Medicine and Science, Los Angeles, California, USA.
Zena
Z
Simmons
Department of MedicineDavid Geffen School of Medicine at UCLA, Los Angeles, California, USA.
Keith C
KC
Norris
Department of Internal MedicineCharles R. Drew University of Medicine and Science, Los Angeles, California, USA.|Department of Health & Life SciencesCharles R. Drew University of Medicine and Science, Los Angeles, California, USA.|Department of MedicineDavid Geffen School of Medicine at UCLA, Los Angeles, California, USA.
Monica G
MG
Ferrini
Department of Internal MedicineCharles R. Drew University of Medicine and Science, Los Angeles, California, USA joartaza@ucla.edu.|Department of Health & Life SciencesCharles R. Drew University of Medicine and Science, Los Angeles, California, USA.|Department of MedicineDavid Geffen School of Medicine at UCLA, Los Angeles, California, USA.
Jorge N
JN
Artaza
FGF; FST; IGF; MSTN; MYC; PAX7; VDR