Improved tympanic membrane regeneration after myringoplastic surgery using an artificial biograft.
Tympanic membrane perforations are due to common otologic problems. The current treatments to heal tympanic membrane perforation, such as myringoplasty, have some disadvantages, including the need for autologous grafting, which is rapidly absorbed by the organism before perforation recovery is complete. To improve the structural and functional tympanic membrane healing after surgery, we propose a new branch of artificial grafts. In this study, we report the development of artificial grafts using electrospun bioabsorbable polymers. Polymers such as poly (l-lactic acid) and poly (lactic-co-glycolic acid) acted as the scaffold for cell growth in a co-culture of fibroblasts and keratinocytes. This co-culture promoted the growth of an epithelial-equivalent tissue over the electrospun scaffold, which was used as an alternative graft in myringoplasty. The in vivo study was performed in Sprague Dawley rats. Ear endoscopy was performed 30days after surgery and showed that tympanic membrane perforations treated with artificial grafts healed naturally, completely and with the possibility of maintaining their actual functionality. In conclusion, our study described a new artificial graft created specifically to fulfill the requirements of perforated tympanic membrane healing processes, which are compatibility, proper durability and less intense side effects following myringoplasty.
Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil.
A P S
APS
Immich
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, Brazil.
P C
PC
Pennacchi
Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil.
A F
AF
Naves
Departamento de Morfologia, Instituto de Biociências de Botucatu, Universidade Estadual Paulista "Júlio de Mesquita Filho", Botucatu, Brazil.
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SL
Felisbino
Centro de Otorrinolaringologia e Fonoaudiologia de São Paulo, Complexo Hospitalar Edmundo Vasconcelos, São Paulo, Brazil.
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RL
Boemo
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, Brazil.
S S
SS
Maria-Engler
Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil. Electronic address: catalani@usp.br.
L H
LH
Catalani
D000818:Animals; D021522:Bioartificial Organs; D016923:Cell Death; D002648:Child; D002675:Child, Preschool; D004724:Endoscopy; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D007633:Keratins / Q000378:metabolism; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D009225:Myringoplasty; D011108:Polymers / Q000737:chemistry; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D013696:Temperature; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry; D014432:Tympanic Membrane / Q000502:physiology* / Q000601:surgery*; D017155:Wettability
D019394:Ki-67 Antigen; D011108:Polymers; D007633:Keratins
Biomimetic material; Cell culture; Fibroblast; In vivo test; Keratinocyte; Poly (lactic-co-glycolic acid)