Rapid quantitation of avenanthramides in oat-containing products by high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (HPLC-TQMS).
Avenanthramides (AVNs) are a family of nitrogen-containing phenolic compounds produced in oat; AVN 2c, 2p, and 2f are the three major members. An LC-MS/MS method was developed, with the limit of detection (LOD) and the limit of quantitation (LOQ) being, respectively, 0.29ng/mL and 1.96ng/mL for AVN 2c, 0.24ng/mL and 0.60ng/mL for AVE 2p, and 0.42ng/mL and 2.2ng/mL for AVN 2f. The method was validated in oat-containing hot cereal and snack bar samples. The recovery of AVN 2c, 2p, and 2f from these two oat products was 95-113%, and the relative standard deviations ranged from 5% to 9%. This method was used to evaluate oat products and raw oat samples. The effects of location and variety on AVN composition were investigated. The method presented here provides a novel and rapid tool to quantitate the abundance of AVN 2c, 2p, and 2f in oat-containing products.
International Chemistry Testing, 258 Main Street, Suite 202, Milford, MA, USA. Electronic address: xie.zhuohong@gmail.com.
Zhuohong
Z
Xie
PepsiCo R&D Analytical Sciences, 617 W. Main Street, Barrington, IL 60010, USA.
Tracy
T
Mui
International Chemistry Testing, 258 Main Street, Suite 202, Milford, MA, USA.
Marsha
M
Sintara
International Chemistry Testing, 258 Main Street, Suite 202, Milford, MA, USA.
Boxin
B
Ou
PepsiCo R&D Nutrition Sciences, 617 W. Main Street, Barrington, IL 60010, USA.
Jodee
J
Johnson
PepsiCo R&D Nutrition Sciences, 617 W. Main Street, Barrington, IL 60010, USA.
YiFang
Y
Chu
PepsiCo R&D Nutrition Sciences, 617 W. Main Street, Barrington, IL 60010, USA.
Marianne
M
O'shea
PepsiCo R&D Analytical Sciences, 617 W. Main Street, Barrington, IL 60010, USA.
Prabhakar
P
Kasturi
PepsiCo R&D Analytical Sciences, 617 W. Main Street, Barrington, IL 60010, USA. Electronic address: Yumin.Chen@pepsico.com.
Yumin
Y
Chen
D018554:Avena / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002523:Edible Grain / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods; D062367:ortho-Aminobenzoates / Q000032:analysis*
D062367:ortho-Aminobenzoates; C514463:avenanthramide-2C
Avenanthramide; HPLC; Mass spectrometry; Multi-reaction monitoring; Oat