Chitosan/β-1,3-glucan/hydroxyapatite bone scaffold enhances osteogenic differentiation through TNF-α-mediated mechanism.
The role of TNF-α in bone healing process is still unclear and controversial. Although it is commonly believed that TNF-α inhibits osteogenic differentiation, there are few reports that identified a crucial role of TNF-α in enhancing bone regeneration process. The aim of this study was to prove that novel chitosan/β-1,3-glucan/HA scaffold (chit/glu/HA) may promote osteogenic differentiation via TNF-α-mediated mechanism and an autocrine stimulation of osteoblasts. It was demonstrated that normal human fetal osteoblasts (hFOB 1.19) maintained in conditioned medium containing increased level of TNF-α and harvested from hFOB 1.19 cells cultured on the chit/glu/HA scaffold (CM-chit/glu/HA) were in more advanced phase of osteogenic differentiation compared to the osteoblasts cultured in non-conditioned osteogenic medium and conditioned medium harvested from hFOB 1.19 cells cultured on the polystyrene plate. Cells cultured in CM-chit/glu/HA produced significantly more Col I protein, revealed 2-fold higher bALP activity, deposited 3-fold more calcium phosphate, and formed mineralized nodules. Thus, it was demonstrated that novel chit/glu/HA scaffold is promising material for bone regeneration applications to stimulate accelerated new bone formation as it enhances osteogenic differentiation via increasing TNF-α production by osteoblasts.
Medical University of Lublin, Chair and Department of Biochemistry and Biotechnology, Chodzki 1 Street, 20-093 Lublin, Poland. Electronic address: agata.przekora@umlub.pl.
Agata
A
Przekora
Medical University of Lublin, Chair and Department of Biochemistry and Biotechnology, Chodzki 1 Street, 20-093 Lublin, Poland.
Grazyna
G
Ginalska
D000469:Alkaline Phosphatase / Q000378:metabolism; D001842:Bone and Bones / Q000187:drug effects*; D002118:Calcium / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects*; D048271:Chitosan / Q000737:chemistry*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D017886:Durapatite / Q000737:chemistry*; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D007477:Ions; D008903:Minerals / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D010012:Osteogenesis / Q000187:drug effects*; D010756:Phosphoric Acids / Q000032:analysis; D054457:Tissue Scaffolds / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism / Q000494:pharmacology*; D047071:beta-Glucans / Q000737:chemistry*
D017077:Culture Media, Conditioned; D015850:Interleukin-6; D007477:Ions; D008903:Minerals; D010756:Phosphoric Acids; D014409:Tumor Necrosis Factor-alpha; D047071:beta-Glucans; D048271:Chitosan; C033363:beta-1,3-glucan; D017886:Durapatite; C030242:phosphoric acid; D000469:Alkaline Phosphatase; D002118:Calcium
Alizarin red S; Bone nodule; Calcium deposition; Curdlan; Direct immunofluorescence