Effect of alcohol dehydrogenase 1C (ADH1C) genotype on vitamin A restriction and marbling in Korean native steers.
OBJECTIVE
This work was to find the correlation of alcohol dehydrogenase 1C (ADH1C) genotype with vitamin A reduction and carcass traits during the vitamin A restriction period.


METHODS
In study 1, 60 Korean native steers were fed a diet (890 IU/kg) with 8,000 IU and 0 IU of supplemental premix vitamin A/kg of dry matter (DM) for control and treatment group, respectively. The levels of serum vitamin A were analyzed through high preparative performance liquid chromatography, and the ADH1C genotype was analyzed based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP; 78.1% TT type, 21.9% TC type); however, CC type was not found. Then, the interaction between ADH1C and carcass traits on the vitamin A restriction was investigated in study 2. A total of 136 Korean native steers were fed a diet that included 930 IU/kg vitamin A of DM.


RESULTS
Serum vitamin A in treatment was reduced to 112.4 IU/dL in steers with TT type of ADH1C, while for steers with TC type the concentration of serum vitamin A was dropped to 79.5 IU/dL (p<0.1) in study 1. This showed that TC type had the potential to lower serum vitamin A concentration during vitamin A restriction compared to TT type. In study 2 we found that eye muscle area, marbling and carcass weight in Korean native steers with TC type were higher than in steers with TT type (p<0.05).


CONCLUSIONS
The interaction between vitamin A restriction and TC type ofADH1Cgene could have the potential of increasing the marbling in Korean native steers. These results indicated that steers with TC type of theADH1Cgene were more sensitive to the change of serum vitamin A than TT types. Furthermore, this finding has the potential to enable a higher marbling score under the condition of vitamin A restriction in Korean native steers.
Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University, Seoul 05029, Korea.|Team of An Educational Program for Specialists in Global Animal Science, Brain Korea 21 Plus Project, Konkuk University, Seoul 05029, Korea.
Dong Qiao
DQ
Peng
Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University, Seoul 05029, Korea.
U Suk
US
Jung
Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University, Seoul 05029, Korea.|Team of An Educational Program for Specialists in Global Animal Science, Brain Korea 21 Plus Project, Konkuk University, Seoul 05029, Korea.
Jae Sung
JS
Lee
Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University, Seoul 05029, Korea.|Team of An Educational Program for Specialists in Global Animal Science, Brain Korea 21 Plus Project, Konkuk University, Seoul 05029, Korea.
Won Seob
WS
Kim
Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University, Seoul 05029, Korea.|Team of An Educational Program for Specialists in Global Animal Science, Brain Korea 21 Plus Project, Konkuk University, Seoul 05029, Korea.
Yong Ho
YH
Jo
Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University, Seoul 05029, Korea.
Min Jeong
MJ
Kim
Animal Nutrition and Physiology team, Department of Animal Biotechnology and Environment, National Institute of Animal Science RDA, Jeonju 54875, Korea.
Young Kun
YK
Oh
Animal Nutrition and Physiology team, Department of Animal Biotechnology and Environment, National Institute of Animal Science RDA, Jeonju 54875, Korea.
Youl Chang
YC
Baek
Department of Animal Life and Environment Science, Hankyong National University, Anseong 17579, Korea.
Seong Gu
SG
Hwang
Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University, Seoul 05029, Korea.|Team of An Educational Program for Specialists in Global Animal Science, Brain Korea 21 Plus Project, Konkuk University, Seoul 05029, Korea.
Hong Gu
HG
Lee
Alcohol Dehydrogenase 1C (ADH1C); Korean Native Steers; Marbling; Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP); Vitamin A Restriction