Vaccinium virgatum fruit extract as an important adjuvant in biochemical and behavioral alterations observed in animal model of metabolic syndrome.
The aim of this study was to investigate the effect of blueberry (Vaccinium virgatum) fruit extract on metabolic, behavioral and oxidative stress parameters in the hippocampus and cerebral cortex of mice submitted to an experimental model of metabolic syndrome induced by a highly palatable diet (HPD). Mice C57BL/6 were divided into 4 experimental groups: (1) received standard chow and saline orally, (2) received standard chow and blueberry hydroalcoholic extract, (3) received HPD and saline orally, (4) received HPD and blueberry hydroalcoholic extract. The animals were treated for 150days. Our results showed that the animals fed with HPD presented insulin resistance, increased body weight, visceral fat, glucose, triglycerides, and total cholesterol when compared to the control group. The blueberry extract prevented the increase of these metabolic parameters. Also, the extract was able to reduce the levels of thiobarbituric acid reactive substances in the cerebral cortex and hippocampus of animals submitted to HPD. In contrast, no differences were observed in the total thiol content, activity of the antioxidant enzymes catalase and superoxide dismutase. In addition, the HPD fed animals showed a significant increase in immobility time in the forced swimming test and blueberry prevented this alteration, although no changes were observed in the ambulatory behavior, as well as in the anxiolytic profile of these animals. Overall, our findings suggest that chronic consumption of blueberry extract exhibits hypoglycemic, hypolipidemic, antidepressant-like and antiperoxidative effects in an animal model of metabolic syndrome.
Laboratório de Biomarcadores, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário s/n, Pelotas, RS, Brazil.
Pathise Souto
PS
Oliveira
Laboratório de Biomarcadores, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário s/n, Pelotas, RS, Brazil.
Marta
M
Gazal
Laboratório de Biomarcadores, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário s/n, Pelotas, RS, Brazil.
Natália Porto
NP
Flores
Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Aline Rigon
AR
Zimmer
Programa de Pós-Graduação em Farmácia, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
Vitor Clasen
VC
Chaves
Programa de Pós-Graduação em Farmácia, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
Flávio Henrique
FH
Reginatto
Departamento de Bioquímica, Universidade Federal de Santa Catarina, Campus Universitário, Córrego Grande, 88040900, Florianópolis, SC, Brazil.
Manuella Pinto
MP
Kaster
Laboratório de Biomarcadores, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário s/n, Pelotas, RS, Brazil.
Rejane Giacomelli
RG
Tavares
Laboratório de Neuroquímica, Inflamação e Câncer, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário s/n, Pelotas, RS, Brazil.
Roselia Maria
RM
Spanevello
Laboratório de Biomarcadores, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário s/n, Pelotas, RS, Brazil. Electronic address: claiton.lencina@ufpel.edu.br.
Claiton Leoneti
CL
Lencina
Laboratório de Biomarcadores, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário s/n, Pelotas, RS, Brazil. Electronic address: francieli.stefanelo@ufpel.edu.br.
Francieli Moro
FM
Stefanello
D000277:Adjuvants, Pharmaceutic / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000872:Anthocyanins / Q000032:analysis; D001007:Anxiety / Q000150:complications / Q000188:drug therapy; D001522:Behavior, Animal / Q000187:drug effects*; D029798:Blueberry Plants / Q000737:chemistry*; D002374:Catalase / Q000378:metabolism; D004032:Diet; D004195:Disease Models, Animal; D005638:Fruit / Q000737:chemistry*; D005951:Glucose Tolerance Test; D006624:Hippocampus / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D024821:Metabolic Syndrome / Q000188:drug therapy* / Q000201:enzymology / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000187:drug effects; D064209:Phytochemicals / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000277:Adjuvants, Pharmaceutic; D000872:Anthocyanins; D064209:Phytochemicals; D010936:Plant Extracts; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D013482:Superoxide Dismutase
Blueberry; Metabolic parameters; Metabolic syndrome; Neurochemical parameters; Phenolic compounds