A green approach to dual-drug nanoformulations with targeting and synergistic effects for cancer therapy.
Exploration of efficient dual-drug nanohybrids, particularly those with high drug loading, specific targeting property, and long-termed stability, is of highly importance in cancer therapy. A pH-driven coprecipitation was performed in the aqueous phase to obtain a dual-drug nanoformulation, composed of 10-hydroxycamptothecine (HCPT) nanoneedles integrated with an exterior thin layer of the methotrexate (MTX)-chitosan conjugate. The high stability of nanohybrids in water and the targeting property provided by the MTX ingredient function synergistically to the prolonged and sustained drug release property in tumor tissues and the increased cellular uptake. The cytotoxicity test illustrates that dual-drug nanoneedles possess the remarkable killing ability to HeLa cells with the combination index at 0.33 ± 0.07. After cellular internalization, the release of both drug ingredients results in an excellent anticancer activity in vivo with the minimized adverse side effects. Design of a green approach to the carrier-free, dual-drug nanoformulations enables to develop emerging drug delivery systems for cancer diagnosis and treatment.
a Institute of Soft Matter and Biomimetics, College of Materials, Xiamen University , Xiamen , China and.
Shichao
S
Wu
a Institute of Soft Matter and Biomimetics, College of Materials, Xiamen University , Xiamen , China and.
Xiangrui
X
Yang
a Institute of Soft Matter and Biomimetics, College of Materials, Xiamen University , Xiamen , China and.
Yue
Y
Lu
b The Department of Physics, Changji University , Changji , China.
Zhongxiong
Z
Fan
a Institute of Soft Matter and Biomimetics, College of Materials, Xiamen University , Xiamen , China and.
Yang
Y
Li
a Institute of Soft Matter and Biomimetics, College of Materials, Xiamen University , Xiamen , China and.
Yuan
Y
Jiang
a Institute of Soft Matter and Biomimetics, College of Materials, Xiamen University , Xiamen , China and.|b The Department of Physics, Changji University , Changji , China.
Zhenqing
Z
Hou
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D001682:Biological Availability; D002166:Camptothecin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004338:Drug Combinations; D004339:Drug Compounding; D004355:Drug Stability; D004357:Drug Synergism; D005260:Female; D055772:Green Chemistry Technology; D006367:HeLa Cells; D006801:Humans; D008727:Methotrexate / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D050997:Nanomedicine; D053758:Nanoparticles; D012995:Solubility; D013678:Technology, Pharmaceutical / Q000379:methods*; D014018:Tissue Distribution; D047368:Tumor Burden / Q000187:drug effects; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
D000970:Antineoplastic Agents; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004338:Drug Combinations; D048271:Chitosan; C028098:10-hydroxycamptothecin; D002166:Camptothecin; D008727:Methotrexate
Dual-drug; anticancer; green; nanoformulation; targeting