Polysaccharides L900/2 and L900/3 isolated from Lactobacillus rhamnosus LOCK 0900 modulate allergic sensitization to ovalbumin in a mouse model.
Here, we compared the abilities of polysaccharides L900/2 and L900/3, which were previously isolated from Lactobacillus rhamnosus LOCK 0900, to modulate the immune response to bystander antigens in a mouse model of ovalbumin (OVA) sensitization. In vivo, both polysaccharides reduced the levels of OVA-specific IgE, IgE-dependent basophil degranulation and IgG2a antibodies, but had no effect on the levels of OVA-specific IgA or IgG1. Interestingly, both polysaccharides triggered recall cellular responses with distinct properties. L900/3 significantly suppressed the OVA-induced upregulations of IL-4, IL-5, IL-10 and IL-13 in re-stimulated spleen cells and mesenteric lymph nodes. Our findings support and expand on our previous in vitro studies by demonstrating that polymer L900/3 might modulate the Th1/Th2 balance and could be a promising candidate molecule for preventing allergic sensitization.
L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114, Wrocław, Poland.
Sabina
S
Górska
Institute of Microbiology, Laboratory of Gnotobiology, Academy of Sciences of the Czech Republic v. v. i., 549 22, Novy Hradek, Czech Republic.
Martin
M
Schwarzer
Institute of Microbiology, Laboratory of Gnotobiology, Academy of Sciences of the Czech Republic v. v. i., 549 22, Novy Hradek, Czech Republic.
Dagmar
D
Srutkova
Institute of Microbiology, Laboratory of Gnotobiology, Academy of Sciences of the Czech Republic v. v. i., 549 22, Novy Hradek, Czech Republic.
Petra
P
Hermanova
L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114, Wrocław, Poland.
Ewa
E
Brzozowska
Institute of Microbiology, Laboratory of Gnotobiology, Academy of Sciences of the Czech Republic v. v. i., 549 22, Novy Hradek, Czech Republic.
Hana
H
Kozakova
L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114, Wrocław, Poland.
Andrzej
A
Gamian
D000485:Allergens / Q000276:immunology*; D000818:Animals; D001491:Basophils / Q000276:immunology; D015550:Cell Degranulation; D016207:Cytokines / Q000096:biosynthesis; D007114:Immunization; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007155:Immunologic Factors / Q000008:administration & dosage* / Q000302:isolation & purification; D052201:Lacticaseibacillus rhamnosus / Q000737:chemistry*; D051379:Mice; D011135:Polysaccharides, Bacterial / Q000008:administration & dosage* / Q000302:isolation & purification; D015843:Serpins / Q000276:immunology*
D000485:Allergens; D016207:Cytokines; D007074:Immunoglobulin G; D007155:Immunologic Factors; D011135:Polysaccharides, Bacterial; C085344:SERPIN-B5; D015843:Serpins; D007073:Immunoglobulin E