Development of efficient adeno-associated virus (AAV)-mediated gene delivery system with a phytoactive material for targeting human melanoma cells.
We exploited the emerging potential of gene therapy strategies to design a powerful therapeutic system that combines two key components-AAV vector and [6]-gingerol. In this study, we created an AAV2 construct expressing the proapoptotic protein BIM, which uses HSPG as its primary receptor, to target HSPG-overexpressing melanoma cells. This combination treatment showed promising results in vitro, inducing apoptosis in human melanoma cells. This new platform technology will make a significant contribution to numerous therapeutic applications, most notably for melanoma, including overcoming resistance to conventional anticancer therapies.
Department of Chemical Engineering, Hanyang University, 222, Wangsimni-ro, Seongdong-gu, Seoul, Republic of Korea.
John Hwan
JH
Lee
Department of Chemical and Biomolecular Engineering, Yonsei University, 50, Yonsei-ro, Seodaemun-gu, Seoul, Republic of Korea.
Yoojin
Y
Kim
Department of Chemical and Biomolecular Engineering, Yonsei University, 50, Yonsei-ro, Seodaemun-gu, Seoul, Republic of Korea.
Ye-Eun
YE
Yoon
Skin Research Division, R&D Unit, AmorePacific Corporation, 1920, Yonggu-daero, Giheung-gu, Yongin-si, Gyeonggi-do, Republic of Korea.
Yong-Jin
YJ
Kim
Department of Chemical Engineering, Hanyang University, 222, Wangsimni-ro, Seongdong-gu, Seoul, Republic of Korea.
Seong-Geun
SG
Oh
Department of Chemical and Biomolecular Engineering, Yonsei University, 50, Yonsei-ro, Seodaemun-gu, Seoul, Republic of Korea.
Jae-Hyung
JH
Jang
Department of Chemical and Biomolecular Engineering, Yonsei University, 50, Yonsei-ro, Seodaemun-gu, Seoul, Republic of Korea; Skin Research Division, R&D Unit, AmorePacific Corporation, 1920, Yonggu-daero, Giheung-gu, Yongin-si, Gyeonggi-do, Republic of Korea. Electronic address: kchem10@gmail.com.
Eunmi
E
Kim
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002396:Catechols / Q000494:pharmacology*; D000229:Dependovirus / Q000235:genetics*; D005233:Fatty Alcohols / Q000494:pharmacology*; D018014:Gene Transfer Techniques; D006801:Humans; D008545:Melanoma / Q000188:drug therapy* / Q000473:pathology; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D002396:Catechols; D005233:Fatty Alcohols; C007845:gingerol
Adeno-associated virus; Gene delivery system; Melanoma; Pro-apoptotic gene; [6]-Gingerol