Microbiological characterization and effect of resin composites in cervical lesions.
BACKGROUND
Non carious cervical lesions associated to muscle hyperfunctions are increasing. Microhybrid resin composites are used to restore cervical abfractions. The purpose of this study was to investigate if resin composites modify tooth plaque, inducing an increment of cariogenic microflora and evaluate their effect,in vivoandin vitro, againstS. mutans.


METHODS
Eight abfractions were restored with two microhybrid resin composites (Venus, Heraeus-Kulzer® and Esthet-X, Dentsply®), after gnatological therapy, in three patients with muscle hyperfunctions. For each abfraction three samples of plaque were taken from the cervical perimeter: before the restoration, one week and three months after restoration. The samples were evaluated both by traditional microbiological methods and by Polymerase Chain Reaction (PCR).In vitro, disk-shaped specimens of the two composites were prepared to estimate the effects against pre-culturedS. mutans, after incubation at 37°C for 24h and assessed by a turbidimetric technique.


RESULTS
In vivono differences were found in plaque growth, for all samples, before and after restoration with both composites;in vitro, instead, a significant reduction ofS. mutansgrowth was found between specimens of two composites (Mann-Whitney U-testp>0,06).


CONCLUSIONS
In this study a relevant consideration was elicited: composite materials,in vivo, do not modify plaque composition of non carious cervical lesions to a potential cariogenic plaque.Key words:Abfraction, restoration, S. mutans, composite, class V.
Institute of Microbiology, Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy.
Bonfanti
B
Carlo
Tutor, Department of Restorative Dentistry, University of Brescia, Italy.
Nicola
N
Barabanti
Institute of Microbiology, Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy.
Giorgio
G
Piccinelli
DDS, MSc. Master of Restorative Dentristy and Endodontics, Department of Stomatology, Medicine and Dental School, Valencia University, Spain.
Vicente
V
Faus-Matoses
Professor, Department of Restorative Dentistry, University of Brescia, Italy.
Antonio
A
Cerutti