Population genomics reveals high gene flow in grass rockfish (Sebastes rastrelliger).
Variation at the genomic level is important for understanding a species' demographic and phylogenetic history, in addition to identifying candidate regions that may be under selection and important to local adaptation. We used restriction-site associated DNA (RAD) sequencing to sample single nucleotide polymorphisms (SNPs) from the grass rockfish genome (Sebastes rastrelliger) to assess range-wide patterns of population structure and test for signatures of selection (i.e. outlier loci). Two different filtering criteria yielded 6572 and 10,393 SNPs that were genotyped in over 70% of 110 individuals from six sampling areas using RAD loci. Global FSTacross sampling sites is 0.0009-0.002 for all loci. Two methods for the detection of outlier loci failed to identify any outlier loci. Clustering analyses and discriminant analysis of principal components were not able to detect any population structure in our dataset. These results suggest high levels of gene flow for this species across its range and that this level of gene flow makes it difficult to detect outlier loci in the current dataset.
Department of Biological Sciences, California State University, 5151 State University Dr., Los Angeles, CA, USA, 90032.
Edith
E
Martinez
Juniata College, 1700 Moore St., Huntingdon, PA, USA, 16652.
Vincent
V
Buonaccorsi
NOAA Fisheries, Southwest Fisheries Science Center, 8901 La Jolla Shores Dr., La Jolla, CA, USA, 92037.
John R
JR
Hyde
Department of Biological Sciences, California State University, 5151 State University Dr., Los Angeles, CA, USA, 90032. Electronic address: aaguil67@calstatela.edu.
Andres
A
Aguilar
D000220:Adaptation, Biological / Q000235:genetics; D000818:Animals; D051456:Gene Flow; D014644:Genetic Variation; D005828:Genetics, Population; D010473:Perciformes / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
F(ST) outliers; Gene flow; Panmixia; RADseq; Single nucleotide polymorphisms