Flavanol Quantification of Grapes via Multiple Reaction Monitoring Mass Spectrometry. Application to Differentiation among Clones of Vitis vinifera L. cv. Rufete Grapes.
The determination of the detailed flavanol composition in food matrices is not a simple task because of the structural similarities of monomers and, consequently, oligomers and polymers. The aim of this study was the development and validation of an HPLC-MS/MS-multiple reaction monitoring (MRM) method that would allow the accurate and precise quantification of catechins, gallocatechins, and oligomeric proanthocyanidins. The high correlation coefficients of the calibration curves (>0.993), the recoveries not statistically different from 100%, the good intra- and interday precisions (<5%), and the LOD and LOQ values, low enough to quantify flavanols in grapes, are good results from the method validation procedure. Its usefulness has also been tested by determining the detailed composition of Vitis vinifera L. cv. Rufete grapes. Seventy-two (38 nongalloylated and 34 galloylated) and 53 (24 procyanidins and 29 prodelphinidins) flavanols have been identified and quantified in grape seed and grape skin, respectively. The use of HCA and PCA on the detailed flavanol composition has allowed differentiation among Rufete clones.
Grupo de Investigación en Polifenoles, Unidad de Nutrición y Bromatologı́a, Facultad de Farmacia, University of Salamanca , Campus Miguel de Unamuno, E-37007 Salamanca, Spain.
Ignacio
http://orcid.org/0000-0001-8794-8328
I
García-Estévez
Grupo de Investigación en Polifenoles, Unidad de Nutrición y Bromatologı́a, Facultad de Farmacia, University of Salamanca , Campus Miguel de Unamuno, E-37007 Salamanca, Spain.
Cristina
http://orcid.org/0000-0002-8526-2442
C
Alcalde-Eon
Grupo de Investigación en Polifenoles, Unidad de Nutrición y Bromatologı́a, Facultad de Farmacia, University of Salamanca , Campus Miguel de Unamuno, E-37007 Salamanca, Spain.
M Teresa
http://orcid.org/0000-0001-6875-2565
MT
Escribano-Bailón
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry / Q000145:classification; D013058:Mass Spectrometry / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry*; D027843:Vitis / Q000737:chemistry* / Q000145:classification
D005419:Flavonoids; D010936:Plant Extracts
HPLC-DAD-MS−multiple reaction monitoring; Vitis vinifera L. cv. Rufete; clonal classification; flavanol; grape seed; grape skin; quantitative mass spectrometry