Chlamydial Lipoproteins Stimulate Toll-Like Receptors 1/2 Mediated Inflammatory Responses through MyD88-Dependent Pathway.
Chlamydiaeare very important pathogens which could cause several types of diseases in human, but little is known about its pathogenic mechanism. In order to elucidate host inflammatory response and the signal pathway induced by Chlamydial lipoproteins, the predicted lipoproteins ofChlamydia trachomatiswere tested for their ability to induce the release of proinflammatory cytokines by mouse macrophages or human TLR (Toll-Like Receptor) expressing cell lines. The results showed that recombinant proteins ofC. trachomatisD381, D541, D067, and D775 displayed a strong ability to induce the release of IL-8 in TLR expressing cell line. The signal pathways involved TLR1/2 and TLR2/CD14 but not TLR4. Moreover, except D067, the proinflammatory cytokine induction by D381, D541, and D775 required the thioacylation site (cysteine) for lipid modification and the induction was through MyD88-mediated pathway. Our data supported that lipoproteins played a vital role in pathogenesis ofC. trachomatis-induced inflammatory responses via TLR pathway. It was the first study to characterize other chlamydial lipoproteins after identifying the role of MIP (D541) on pathogenesis of Chlamydial diseases.
School of Basic Medical Sciences, Xiangya School of Medicine, Central South University Changsha, China.
Yong
Y
Wang
Department of Cardiovascular Diseases, Xiangya Hospital, Central South University Changsha, China.
Qiong
Q
Liu
Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio San Antonio, TX, USA.
Ding
D
Chen
School of Basic Medical Sciences, Xiangya School of Medicine, Central South University Changsha, China.
Jie
J
Guan
School of Basic Medical Sciences, Xiangya School of Medicine, Central South University Changsha, China.
Linghui
L
Ma
Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio San Antonio, TX, USA.
Guangming
G
Zhong
School of Basic Medical Sciences, Xiangya School of Medicine, Central South University Changsha, China.
Hengping
H
Shu
School of Basic Medical Sciences, Xiangya School of Medicine, Central South University Changsha, China.
Xiang
X
Wu
Chlamydial trachoma; Toll-Like Receptor; cytokine; immune response; lipoproteins