De novotranscriptome assembly of heavy metal tolerantSilene dioica.
Silene dioicais a dioecious plant of the familyCaryophyllaceae. In the present study, we used Illumina sequencing technology (MiSeq) to sequence,de novoassembly and annotate the transcriptomes of male and female copper tolerantS. dioicaindividuals. We sequenced the normalized mRNA of roots, shoots, flower buds and flowers for each sex. Raw reads of the transcriptome assembly project forS. dioicamale and female individual have been deposited in NCBI's Sequence Read Archive (SRA) database with the accession number SRP094611. The Trinity and Detonate program was used tode novoassembly 92,347 transcripts for male and 94,757 transcripts for female transcriptome. The assembled transcriptome sequences forS. dioicamale and female individuals can be accessed at NCBI with the following accession numbers: GFCG00000000 (male); GFCH00000000 (female). The obtained transcriptomic data will be useful for further studies focusing on copper tolerance, comparative transcriptome analysis with otherSilenespecies and sex chromosomes evolution.
Department of Plant Developmental Genetics, Institute of Biophysics of the CAS, v.v.i., Kralovopolska 135, CZ-612 00 Brno, Czech Republic.
Radim
R
Cegan
Department of Plant Developmental Genetics, Institute of Biophysics of the CAS, v.v.i., Kralovopolska 135, CZ-612 00 Brno, Czech Republic.
Vojtech
V
Hudzieczek
Department of Plant Developmental Genetics, Institute of Biophysics of the CAS, v.v.i., Kralovopolska 135, CZ-612 00 Brno, Czech Republic; Centre of Plant Structural and Functional Genomics, Institute of Experimental Botany of the CAS, v.v.i., Slechtitelu 31, CZ-78371 Olomouc-Holice, Czech Republic.
Roman
R
Hobza
Heavy metal tolerance; RNA-Seq; Sex chromosomes; Silene dioica; Transcriptome