Expression and localization of ARTEMIN in the bovine uterus and embryos.
Artemin a member of the glial cell line-derived neurotrophic factor (GDNF) family is present in mice and human preimplantation embryos, and reproductive tract, during early pregnancy promoting embryo development in vitro. The presence of artemin in cattle embryos and reproductive tract, however, is unknown. In the present work we identified for first time artemin in bovine uterine fluid (UF) (Western blot), endometrium (RT-PCR, Western blot and immunohistochemistry) and embryos (RT-PCR and immunohistochemistry) during early preimplantation development. In addition, GFRalpha3, a component of the artemin receptor was localized in blastocysts produced in vitro. Individually developing embryos released ARTEMIN in culture medium and triggered ARTEMIN mRNA down-regulation in epithelial cells from endometrial cell cultures. Our results suggest that ARTEMIN derived from early embryos and maternal reproductive tract may exert important roles during early development in cattle.
Centro de Biotecnología Animal, SERIDA, Camino de Rioseco 1225, 33394, Gijón, Spain.
E
E
Gómez
Centro de Biotecnología Animal, SERIDA, Camino de Rioseco 1225, 33394, Gijón, Spain.
D
D
Martin
Centro de Biotecnología Animal, SERIDA, Camino de Rioseco 1225, 33394, Gijón, Spain.
S
S
Carrocera
INIA Departamento de Reproducción Animal y Conservación de Recursos Zoogenéticos, Cra de La Coruña Km 5600, 28040, Madrid, Spain.
M J
MJ
Sánchez-Calabuig
INIA Departamento de Reproducción Animal y Conservación de Recursos Zoogenéticos, Cra de La Coruña Km 5600, 28040, Madrid, Spain.
A
A
Gutierrez-Adán
Unidad de Microscopía Fotónica y Proceso de Imágenes, Servicios Científico Técnicos, Universidad de Oviedo, Instituto Universitario de Oncología de Asturias (IUOPA), 33006, Oviedo, Spain.
M
M
Alonso-Guervos
INRA, UMR1198 Biologie du Développement et Reproduction, F-78350, Jouy-en-Josas, France.
N
N
Peynot
INRA, UMR1198 Biologie du Développement et Reproduction, F-78350, Jouy-en-Josas, France.
C
C
Giraud-Delville
INRA, UMR1198 Biologie du Développement et Reproduction, F-78350, Jouy-en-Josas, France.
O
O
Sandra
INRA, UMR1198 Biologie du Développement et Reproduction, F-78350, Jouy-en-Josas, France.
V
V
Duranthon
Centro de Biotecnología Animal, SERIDA, Camino de Rioseco 1225, 33394, Gijón, Spain. Electronic address: mmunoz@serida.org.
M
M
Muñoz
D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D002417:Cattle; D047108:Embryonic Development; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000096:biosynthesis* / Q000235:genetics; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis; D017475:Receptors, Nerve Growth Factor / Q000096:biosynthesis* / Q000235:genetics
D051100:Glial Cell Line-Derived Neurotrophic Factor; D012333:RNA, Messenger; D017475:Receptors, Nerve Growth Factor
ARTEMIN; Cattle; Early embryonic development; Immunohistochemistry; mRNA