Connexin40 correlates with oxidative stress in brains of traumatic brain injury rats.
Oxidative stress is an important factor in the pathophysiologic changes after traumatic brain injury (TBI). Connexin43 (Cx43) was reported to contribute to cerebral damage. However, the impacts of Cx40 have not been investigated in detail.



In the present study, we hypothesized that Cx40 was involved in oxidative stress-induced brain injury after TBI.



The controlled cortical impact (CCI) model was introduced to Wistar rats as a TBI model. Neurological deficits, oxidative stress and Cx40 were evaluated in TBI rats and N-acetylcysteine (NAC)-treated TBI rats. Neurological severity score (NSS) was used to assess neurological deficits. Brain infarction was measured by histo-staining. Brain edema was evaluated by measuring the brain water content. Cortex samples were collected to measure the tissue levels of malonyldialdehyde (MDA), nitric oxide (NO) and glutathione (GSH) and NADPH oxidase activity. Cx40 expression was determined by Western-blot.



TBI-induced brain injuries gradually increased from 6 h to 24 h post CCI, and the severity remained till 72 h. The level of oxidative stress was consistent with the extent of neurological deficits. Cx40 was upregulated after TBI in a linear correlated manner with increased oxidative stress. With NAC intervention, both neurological deficits and oxidative stress were significantly attenuated. Meanwhile, elevated Cx40 expression in cortex was also prevented by NAC treatment.



These studies revealed the relationship between levels of Cx40 and oxidative stress after TBI. The cortex Cx40 expression was positively correlated with the cerebral oxidative stress, indicating the involvement of Cx40 in the progress of brain damage.
Wei
W
Chen
Yijun
Y
Guo
Wenjin
W
Yang
Ping
P
Zheng
Jinsong
J
Zeng
Wusong
W
Tong
D000111:Acetylcysteine / Q000627:therapeutic use; D000704:Analysis of Variance; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D001929:Brain Edema / Q000209:etiology; D020520:Brain Infarction / Q000209:etiology; D000070642:Brain Injuries, Traumatic / Q000188:drug therapy / Q000473:pathology* / Q000503:physiopathology*; D017630:Connexins / Q000378:metabolism*; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D000097003:Gap Junction alpha-5 Protein
D000111:Acetylcysteine; D017630:Connexins; D000097003:Gap Junction alpha-5 Protein
Traumatic brain injury (TBI); connexin40; oxidative stress