Heme-binding activity of methoxyflavones from Pentzia monodiana Maire (Asteraceae).
A heme-binding assay based on mass spectrometry was performed on P. monodiana Maire (Asteraceae) extracts to identify metabolites able to form adducts with heminic part of haemoglobin, as potential antimalarial drugs. Main adducts were characterized and their stability was measured. Isolation of main constituents of P. monodiana Maire lead to identification of the two methoxyflavones 3'-O-methyleupatorin (7) and artemetin (8) involved in the adducts formation. Four seco-tanapartholides (1-4), a guaianolide (5), a germacranolide (6) and two other methoxyflavones (9, 10) were also characterized. Evaluation of isolated compounds on P. falciparum and T. brucei brucei showed a moderate antiprotozoal activity of the two methoxyflavones.
Laboratoire de Pharmacognosie, UMR CNRS 8638 COMETE, Faculté de Pharmacie de Paris, Université Paris-Descartes, Sorbonne Paris Cité, 4, av. de l'Observatoire, 75006 Paris, France. Electronic address: sergio.ortiz.aguirre@gmail.com.
Sergio
S
Ortiz
Département de Pharmacie, Faculté de Médecine, Université de Tlemcen, 13000 Tlemcen, Algérie. Electronic address: kameldali@mail.univ-tlemcen.dz.
Kamel
K
Dali-Yahia
BioCIS, Univ. Paris-Sud, CNRS, Université Paris-Saclay, 5 rue J.-B. Clément, 92290 Châtenay-Malabry, France. Electronic address: pedro.vasquez-ocmin@u-psud.fr.
Pedro
P
Vasquez-Ocmin
Laboratoire de Pharmacognosie, UMR CNRS 8638 COMETE, Faculté de Pharmacie de Paris, Université Paris-Descartes, Sorbonne Paris Cité, 4, av. de l'Observatoire, 75006 Paris, France. Electronic address: raphael.grougnet@parisdescartes.fr.
Raphaël
R
Grougnet
Unité Molécules de Communication et Adaptation des Microorganismes (MCAM, UMR 7245), Muséum National d'Histoire Naturelle, CNRS, Sorbonne Universités, CP52, 57 rue Cuvier, 75005 Paris, France. Electronic address: grellier@mnhn.fr.
Philippe
P
Grellier
Laboratoire de Pharmacognosie, UMR CNRS 8638 COMETE, Faculté de Pharmacie de Paris, Université Paris-Descartes, Sorbonne Paris Cité, 4, av. de l'Observatoire, 75006 Paris, France. Electronic address: sylvie.michel@parisdescartes.fr.
Sylvie
S
Michel
BioCIS, Univ. Paris-Sud, CNRS, Université Paris-Saclay, 5 rue J.-B. Clément, 92290 Châtenay-Malabry, France. Electronic address: alexandre.maciuk@u-psud.fr.
Alexandre
A
Maciuk
Laboratoire de Pharmacognosie, UMR CNRS 8638 COMETE, Faculté de Pharmacie de Paris, Université Paris-Descartes, Sorbonne Paris Cité, 4, av. de l'Observatoire, 75006 Paris, France. Electronic address: sabrina.boutefnouchet@parisdescartes.fr.
Sabrina
S
Boutefnouchet
D000462:Algeria; D000981:Antiprotozoal Agents / Q000737:chemistry* / Q000302:isolation & purification; D019659:Asteraceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D047309:Flavones / Q000737:chemistry* / Q000302:isolation & purification; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification; D006454:Hemoglobins / Q000737:chemistry*; D013058:Mass Spectrometry; D015394:Molecular Structure; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D010963:Plasmodium falciparum / Q000187:drug effects; D045788:Sesquiterpenes, Germacrane / Q000737:chemistry / Q000302:isolation & purification; D014346:Trypanosoma brucei brucei / Q000187:drug effects
D000981:Antiprotozoal Agents; D047309:Flavones; D005419:Flavonoids; D006454:Hemoglobins; D010936:Plant Extracts; D045788:Sesquiterpenes, Germacrane; C432449:germacranolide; C054132:artemetin