Surface modification of TiO2nanotubes with osteogenic growth peptide to enhance osteoblast differentiation.
To investigate the influence of surface-biofunctionalized substrates on osteoblast behavior, a layer of aligned TiO2nanotubes with a diameter of around 70nm was fabricated on titanium surface by anodization, and then osteogenic growth peptide (OGP) was conjugated onto TiO2nanotubes through the intermediate layer of polydopamine. The morphology, composition and wettability of different surfaces were characterized by field-emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and contact angle measurements, respectively. The effects of OGP-modified TiO2nanotube substrates on the morphology, proliferation and differentiation of osteoblasts were examined in vitro. Immunofluorescence staining revealed that the OGP-functionalized TiO2nanotubes were favorable for cell spreading. However, there was no significant difference in cell proliferation observed among the different groups. Cells grown onto OGP-functionalized TiO2nanotubes showed significantly higher (p<0.05 or p<0.01) levels of alkaline phosphatase (ALP) and mineralization after 4, 7 and 14days of culture, respectively. Cells grown on OGP-functionalized TiO2nanotubes had significantly higher (p<0.05 or p<0.01) expression of osteogenic-related genes including runt related transcription factor 2 (Runx2), ALP, collagen type I (Col I), osteopontin (OPN) and osteocalcin (OC) after 14days of culture. These data suggest that surface functionalization of TiO2nanotubes with OGP was beneficial for cell spreading and differentiation. This study provides a novel platform for the development and fabrication of titanium-based implants that enhance the propensity for osseointegration between the native tissue and implant interface.
School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, China. Electronic address: minlai@jsnu.edu.cn.
Min
M
Lai
School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, China.
Ziyang
Z
Jin
Department of Pharmacy, The Affiliated hospital of Qingdao University, Qingdao, Shandong 266555, China.
Zhiguo
Z
Su
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D006657:Histones / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D010012:Osteogenesis / Q000187:drug effects; D056951:Photoelectron Spectroscopy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology*
D006657:Histones; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; C074533:osteogenic growth peptide; C009495:titanium dioxide; D014025:Titanium; D000469:Alkaline Phosphatase
Differentiation; Osteoblast; Osteogenic growth peptide; TiO(2) nanotubes