Stem cell-extracellular vesicles as drug delivery systems: New frontiers for silk/curcumin nanoparticles.
The aim of this work was to develop a novel carrier-in-carrier system based on stem cell-extracellular vesicles loaded of silk/curcumin nanoparticles by endogenous technique. Silk nanoparticles were produced by desolvation method and curcumin has been selected as drug model because of its limited water solubility and poor bioavailability. Nanoparticles were stable, with spherical geometry, 100nm in average diameter and the drug content reached about 30%. Cellular uptake studies, performed on mesenchymal stem cells (MSCs), showed the accumulation of nanoparticles in the cytosol around the nuclear membrane, without cytotoxic effects. Finally, MSCs were able to release extracellular vesicles entrapping silk/curcumin nanoparticles. This combined biological-technological approach represents a novel class of nanosystems, combining beneficial effects of both regenerative cell therapies and pharmaceutical nanomedicine, avoiding the use of viable replicating stem cells.
University of Pavia, Department of Drug Sciences, Viale Taramelli 12, 27100 Pavia, Italy. Electronic address: sara.perteghella@unipv.it.
Sara
S
Perteghella
University of Pavia, Department of Drug Sciences, Viale Taramelli 12, 27100 Pavia, Italy. Electronic address: barbara.crivelli@unipv.it.
Barbara
B
Crivelli
University of Pavia, Department of Drug Sciences, Viale Taramelli 12, 27100 Pavia, Italy. Electronic address: laura.catenacci@unipv.it.
Laura
L
Catenacci
University of Pavia, Department of Drug Sciences, Viale Taramelli 12, 27100 Pavia, Italy. Electronic address: milena.sorrenti@unipv.it.
Milena
M
Sorrenti
University of Pavia, Department of Chemistry, Viale Taramelli 16, 27100 Pavia, Italy. Electronic address: giovanna.bruni@unipv.it.
Giovanna
G
Bruni
University of Pavia, Department of Molecular Medicine, Via Forlanini 6, 27100 Pavia, Italy; University of Pavia, Centro Grandi Strumenti, Via Bassi 21, 27100 Pavia, Italy. Electronic address: vittorio.necchi@unipv.it.
Vittorio
V
Necchi
University of Pavia, Department of Drug Sciences, Viale Taramelli 12, 27100 Pavia, Italy. Electronic address: barbara.vigani@unipv.it.
Barbara
B
Vigani
SUPSI, University of Applied Sciences and Arts of Southern Switzerland, Innovative Technologies Department, Via Pobiette 11, 6928 Manno, Switzerland. Electronic address: marzio.sorlini@supsi.ch.
Marzio
M
Sorlini
University of Pavia, Department of Drug Sciences, Viale Taramelli 12, 27100 Pavia, Italy. Electronic address: marina.torre@unipv.it.
Maria Luisa
ML
Torre
University of Pavia, Department of Drug Sciences, Viale Taramelli 12, 27100 Pavia, Italy. Electronic address: theodora.chlapanidas@unipv.it.
Theodora
T
Chlapanidas
D002470:Cell Survival / Q000187:drug effects; D003474:Curcumin / Q000737:chemistry / Q000493:pharmacokinetics*; D016503:Drug Delivery Systems / Q000379:methods*; D065546:Drug Liberation; D004355:Drug Stability; D000067128:Extracellular Vesicles / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D010316:Particle Size; D047011:Silk / Q000737:chemistry / Q000493:pharmacokinetics*; D013234:Stem Cells / Q000166:cytology*
D047011:Silk; D003474:Curcumin
Curcumin; Drug delivery systems; Extracellular vesicles; Mesenchymal stem cells; Nanoparticles; Silk fibroin