Methyl Salicylate Level Increase in Flax afterFusarium oxysporumInfection Is Associated with Phenylpropanoid Pathway Activation.
Flax (Linum usitatissimum) is a crop plant valued for its oil and fiber. Unfortunately, large losses in cultivation of this plant are caused by fungal infections, withFusarium oxysporumbeing one of its most dangerous pathogens. Among the plant's defense strategies, changes in the expression of genes of the shikimate/phenylpropanoid/benzoate pathway and thus in phenolic contents occur. Among the benzoates, salicylic acid, and its methylated form methyl salicylate play an important role in regulating plants' response to stress conditions. Upon treatment of flax plants with the fungus we found that methyl salicylate content increased (4.8-fold of the control) and the expression profiles of the analyzed genes suggest that it is produced most likely from cinnamic acid, through the β-oxidative route. At the same time activation of some genes involved in lignin and flavonoid biosynthesis was observed. We suggest that increased methyl salicylate biosynthesis during flax response toF. oxysporuminfection may be associated with phenylpropanoid pathway activation.
Faculty of Biotechnology, University of Wrocław Wrocław, Poland.
Aleksandra
A
Boba
Faculty of Biotechnology, University of Wrocław Wrocław, Poland.
Kamil
K
Kostyn
Department of Genetics, Institute of Genetics and Microbiology, University of Wroclaw Wroclaw, Poland.
Anna
A
Kostyn
Faculty of Biotechnology, University of WrocławWrocław, Poland; Department of Genetics, Plant Breeding and Seed Production, Faculty of Life Sciences and Technology, Wroclaw University of Environmental and Plant SciencesWroclaw, Poland.
Wioleta
W
Wojtasik
Department of Food Science and Dietetics, Medical University of Wroclaw Wroclaw, Poland.
Mariusz
M
Dziadas
Faculty of Biotechnology, University of Wrocław Wrocław, Poland.
Marta
M
Preisner
Faculty of Biotechnology, University of WrocławWrocław, Poland; Department of Genetics, Plant Breeding and Seed Production, Faculty of Life Sciences and Technology, Wroclaw University of Environmental and Plant SciencesWroclaw, Poland.
Jan
J
Szopa
Faculty of Biotechnology, University of Wrocław Wrocław, Poland.
Anna
A
Kulma
Fusarium oxysporum; benzoate; flax; phenylpropanoids; salicylic acid