Phytochemical composition of fractions isolated from ten Salvia species by supercritical carbon dioxide and pressurized liquid extraction methods.
Ten Salvia species, S. amplexicaulis, S. austriaca, S. forsskaolii S. glutinosa, S. nemorosa, S. officinalis, S. pratensis, S. sclarea, S. stepposa and S. verticillata were fractionated using supercritical carbon dioxide and pressurized liquid (ethanol and water) extractions. Fifteen phytochemicals were identified using commercial standards (some other compounds were identified tentatively), 11 of them were quantified by ultra high pressure chromatography (UPLC) with quadruple and time-of-flight mass spectrometry (Q/TOF, TQ-S). Lipophilic CO2extracts were rich in tocopherols (2.36-10.07mg/g), while rosmarinic acid was dominating compound (up to 30mg/g) in ethanolic extracts. Apigenin-7-O-β-d-glucuronide, caffeic and carnosic acids were quantitatively important phytochemicals in the majority other Salvia spp. Antioxidatively active constituents were determined by using on-line high-performance liquid chromatography (HPLC) analysis combined with 2,2'-diphenyl-1-picrylhydrazyl (DPPH) assay (HPLC-DPPH). Development of high pressure isolation process and comprehensive characterisation of phytochemicals in Salvia spp. may serve for their wider applications in functional foods and nutraceuticals.
Department of Food Science and Technology, Kaunas University of Technology, Radvilėnų pl. 19, LT-50254, Lithuania.
Vaida
V
Šulniūtė
Department of Food Science and Technology, Kaunas University of Technology, Radvilėnų pl. 19, LT-50254, Lithuania.
Audrius
A
Pukalskas
Department of Food Science and Technology, Kaunas University of Technology, Radvilėnų pl. 19, LT-50254, Lithuania. Electronic address: rimas.venskutonis@ktu.lt.
Petras Rimantas
PR
Venskutonis
D001713:Biphenyl Compounds / Q000032:analysis; D002245:Carbon Dioxide; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D064209:Phytochemicals / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry*; D027544:Salvia / Q000737:chemistry*
D001713:Biphenyl Compounds; D064209:Phytochemicals; D010936:Plant Extracts; D002245:Carbon Dioxide
Polyphenols; Pressurized liquid extraction; Salvia spp.; Supercritical carbon dioxide extraction; Tocopherols