Biodegradation of Phenanthrene in Polycyclic Aromatic Hydrocarbon-Contaminated Wastewater Revealed by Coupling Cultivation-Dependent and -Independent Approaches.
The indigenous microorganisms responsible for degrading phenanthrene (PHE) in polycyclic aromatic hydrocarbons (PAHs)-contaminated wastewater were identified by DNA-based stable isotope probing (DNA-SIP). In addition to the well-known PHE degraders Acinetobacter and Sphingobium, Kouleothrix and Sandaracinobacter were found, for the first time, to be directly responsible for indigenous PHE biodegradation. Additionally, a novel PHE degrader, Acinetobacter tandoii sp. LJ-5, was identified by DNA-SIP and direct cultivation. This is the first report and reference to A. tandoii involved in the bioremediation of PAHs-contaminated water. A PAH-RHDαgene involved in PHE metabolism was detected in the heavy fraction of13C treatment, but the amplification of PAH-RHDαgene failed in A. tandoii LJ-5. Instead, the strain contained catechol 1,2-dioxygenase and the alpha/beta subunits of protocatechuate 3,4-dioxygenase, indicating use of the β-ketoadipate pathway to degrade PHE and related aromatic compounds. These findings add to our current knowledge on microorganisms degrading PHE by combining cultivation-dependent and cultivation-independent approaches and provide deeper insight into the diversity of indigenous PHE-degrading communities.
Guangzhou Institute of Geochemistry, Chinese Academy of Sciences , Guangzhou 510640, China.|University of Chinese Academy of Sciences , Beijing, 100039, China.
Jibing
J
Li
Guangzhou Institute of Geochemistry, Chinese Academy of Sciences , Guangzhou 510640, China.
Chunling
0000-0003-2359-4246
C
Luo
Guangzhou Institute of Geochemistry, Chinese Academy of Sciences , Guangzhou 510640, China.
Mengke
M
Song
Guangzhou Institute of Geochemistry, Chinese Academy of Sciences , Guangzhou 510640, China.|University of Chinese Academy of Sciences , Beijing, 100039, China.
Qing
Q
Dai
Guangzhou Institute of Geochemistry, Chinese Academy of Sciences , Guangzhou 510640, China.
Longfei
L
Jiang
Lancaster Environment Centre, Lancaster University , Lancaster, LA1 4YQ, United Kingdom.
Dayi
0000-0002-4175-5982
D
Zhang
Guangzhou Institute of Geochemistry, Chinese Academy of Sciences , Guangzhou 510640, China.
Gan
G
Zhang
D001673:Biodegradation, Environmental; D010616:Phenanthrenes / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism; D062065:Wastewater
D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants; D062065:Wastewater