Rce1 expression in renal cell carcinoma and its regulatory effect on 786-O cell apoptosis through endoplasmic reticulum stress.
Ras and a-factor-converting enzyme 1 (Rce1) is located in the endoplasmic reticulum (ER) and is thought to be responsible for endoproteolytic processing of the vast majority of CAAX proteins. Endoplasmic reticulum stress (ERS) plays an important role in renal cell carcinoma (RCC); however, the expression and role of Rce1 in RCC have not been extensively studied. We aimed to investigate the expression of Rce1 in RCC tissues and its molecular mechanism in ERS-induced apoptosis in RCC 786-O cells. We first used western blotting, quantitative reverse transcriptase-polymerase chain reaction, and immunohistochemistry to detect the Rce1 expression in renal carcinoma tissues and paracancerous tissues. It was found that Rce1 expression was upregulated in RCC tissues, and its positive expression level was strongly associated with clinicopathologic features. Next, we detected the expression of Rce1 in human embryonic kidney cell line HEK293 and human renal carcinoma cell lines 786-O, ACHN, and A498. Higher expression of Rce1 was found in human renal carcinoma cell lines, especially in 786-O cells. Knockdown of Rce1 in 786-O cells increased apoptosis and inhibited proliferation (P < 0.05). Moreover, downregulation of Rce1 upregulated the expression of the pro-apoptotic protein Bax, but downregulated the expression of the anti-apoptotic protein Bcl-2. Further studies showed that downregulation of Rce1 also affected the expression of ERS factors. In conclusion, our results indicated that Rce1 plays a key role in RCC. Low expression of Rce1 might indirectly increase apoptosis and inhibit proliferation of renal carcinoma cells through ERS.
Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Jianjun
J
Li
Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Delin
D
Wang
Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Junnan
J
Liu
Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Yunlang
Y
Qin
Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Liangliang
L
Huang
Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Qiangfeng
Q
Zeng
Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Maolin
M
Xiao
Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Jie
J
Hu
Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Qixin
Q
Yang
Gastroenterology and Neurology Center, University-Town Hospital of Chongqing Medical University, Chongqing 401331, China.
Jiang
J
He
Department of Biochemistry and Molecular Biology, Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China.
Li
L
Mai
College of Life Science, Chongqing Medical University, Chongqing 400016, China.
Ying
Y
Li
Institute of Urology, The First Affiliated Hospital of Peking University, Beijing 100000, China.
Wujiang
W
Liu
D051701:Activating Transcription Factor 4 / Q000235:genetics / Q000378:metabolism; D017209:Apoptosis; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D016022:Case-Control Studies; D049109:Cell Proliferation; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism / Q000473:pathology; D059865:Endoplasmic Reticulum Stress; D015852:Eukaryotic Initiation Factor-2 / Q000235:genetics / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D019892:eIF-2 Kinase / Q000235:genetics / Q000378:metabolism
C497109:ATF4 protein, human; C000595707:BCL2 protein, human; D015852:Eukaryotic Initiation Factor-2; D019253:Proto-Oncogene Proteins c-bcl-2; D051701:Activating Transcription Factor 4; C460640:EIF2AK3 protein, human; D019892:eIF-2 Kinase; D010450:Endopeptidases; C491326:RCE1 protein, human
Ras and a-factor-converting enzyme 1; apoptosis; endoplasmic reticulum stress; renal cell carcinoma