Small RNAs regulate the biocontrol property of fluorescent Pseudomonas strain Psd.
The production of biocontrol factors by Pseudomonads is reported to be controlled at the post-transcriptional level by the GacS/GacA signal transduction pathway. This involves RNA-binding translational repressor proteins, RsmA and RsmE, and the small regulatory RNAs (sRNAs) RsmX, RsmY, and RsmZ. While the former represses genes involved in secondary metabolite production, the latter relieves this repression at the end of exponential growth. We have studied the fluorescent Pseudomonas strain Psd, possessing good biocontrol potential, and confirmed the presence of rsmY and rsmZ by PCR amplification. Gene constructs for all the three small RNAs (RsmX, RsmY and RsmZ) carried on broad host-range plasmid, pME6032 were mobilized into strain Psd. Expression analysis of gacA in the recombinant strains over-expressing rsmX (Psd-pME7320), rsmY (Psd-pME6359) and rsmZ (Psd-pME6918) revealed a significant upregulation of the response regulator. Besides, a remarkable down-regulation of rsmA was also reported in all the strains. The variant strains were found to produce comparatively higher levels of phenazines. Indole acetic acid levels were higher to some extent, and strain Psd-pME6918 also showed elevated production of HCN. The tomato seedlings infected with Fusarium oxysporum and Verticillium dahliae in the presence of culture filtrate of the recombinant strains showed better plant protection response in comparison to the wild-type strain Psd. These results suggest that small RNAs are important determinants in regulation of the biocontrol property of strain Psd.
Department of Genetics, University of Delhi South Campus, Benito Juarez Marg, New Delhi, 110 021, India.
Anamika
A
Upadhyay
TERI Deakin Nanobiotechnology Centre, The Energy and Resources Institute (TERI), Gual Pahari, Gurgaon Faridabad Road, Gurgaon, Haryana 122 001, India.
Mandira
M
Kochar
Wadsworth Center, NYS Department of Health, Empire State Plaza, P.O. Box 509, Albany, New York 12201-0509, USA.
Ashutosh
A
Upadhyay
Department of Genetics, University of Delhi South Campus, Benito Juarez Marg, New Delhi, 110 021, India.
Soumya
S
Tripathy
Department of Genetics, University of Delhi South Campus, Benito Juarez Marg, New Delhi, 110 021, India.
Manchikatla Venkat
MV
Rajam
Department of Genetics, University of Delhi South Campus, Benito Juarez Marg, New Delhi, 110 021, India. Electronic address: sslab222012@gmail.com.
Sheela
S
Srivastava
D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D005670:Fusarium / Q000187:drug effects / Q000472:pathogenicity; D007210:Indoleacetic Acids / Q000378:metabolism; D018551:Solanum lycopersicum / Q000187:drug effects / Q000382:microbiology; D010572:Pest Control, Biological; D010619:Phenazines / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control; D011549:Pseudomonas / Q000235:genetics* / Q000378:metabolism*; D012329:RNA, Bacterial / Q000235:genetics* / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000382:microbiology; D017422:Sequence Analysis, DNA; D017262:Siderophores / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D020172:Verticillium / Q000187:drug effects / Q000472:pathogenicity
D000935:Antifungal Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C091100:GacA protein, Bacteria; D007210:Indoleacetic Acids; D010619:Phenazines; D012329:RNA, Bacterial; D016601:RNA-Binding Proteins; D017262:Siderophores; D014157:Transcription Factors; C030737:indoleacetic acid
Antibiotic production; Biocontrol; Fungal pathogens; Regulation; Small regulatory RNAs