In vitro stimulation of vascular endothelial growth factor by borate-based glass fibers under dynamic flow conditions.
Bioactive borate glass has been recognized to have both hard and soft tissue repair and regeneration capabilities through stimulating both osteogenesis and angiogenesis. However, the underlying biochemical and cellular mechanisms remain unclear. In this study, dynamic flow culturing modules were designed to simulate the micro-environment near the vascular depletion and hyperplasia area in wound-healing regions, thus to better investigate the mechanisms underlying the biocompatibility and functionality of borate-based glass materials. Glass fibers were dosed either upstream or in contact with the pre-seeded cells in the dynamic flow module. Two types of borate glasses, doped with (1605) or without (13-93B3) CuO and ZnO, were studied along with the silicate-based glass, 45S5. Substantial fiber dissolution in cell culture medium was observed, leading to the release of ions (boron, sodium and potassium) and the deposition of a calcium phosphate phase. Different levels of vascular endothelial growth factor secretion were observed from cells exposed to these three glass fibers, and the copper/zinc containing borate 1605 fibers exhibited the most positive influence. These results indicate that dynamic studies of in vitro bioactivity provide useful information to understand the in vivo response to bioactive borate glasses.
Department of Chemistry, Missouri University of Science and Technology, Rolla, MO 65409, USA; Center for Biomedical Science and Engineering, Missouri University of Science and Technology, Rolla, MO 65409, USA.
Sisi
S
Chen
Department of Chemistry, Missouri University of Science and Technology, Rolla, MO 65409, USA; Center for Biomedical Science and Engineering, Missouri University of Science and Technology, Rolla, MO 65409, USA; Center for Single Nanoparticle, Single Cell, and Single Molecule Monitoring, Missouri University of Science and Technology, Rolla, MO 65409, USA.
Qingbo
Q
Yang
Department of Material Science and Engineering, Missouri University of Science and Technology, Rolla, MO 65409, USA; Center for Biomedical Science and Engineering, Missouri University of Science and Technology, Rolla, MO 65409, USA.
Richard K
RK
Brow
Department of Chemistry, Missouri University of Science and Technology, Rolla, MO 65409, USA; Center for Single Nanoparticle, Single Cell, and Single Molecule Monitoring, Missouri University of Science and Technology, Rolla, MO 65409, USA.
Kun
K
Liu
Department of Chemistry, Missouri University of Science and Technology, Rolla, MO 65409, USA.
Katherine A
KA
Brow
Department of Chemistry, Missouri University of Science and Technology, Rolla, MO 65409, USA; Center for Biomedical Science and Engineering, Missouri University of Science and Technology, Rolla, MO 65409, USA; Center for Single Nanoparticle, Single Cell, and Single Molecule Monitoring, Missouri University of Science and Technology, Rolla, MO 65409, USA.
Yinfa
Y
Ma
Department of Chemistry, Missouri University of Science and Technology, Rolla, MO 65409, USA; Center for Biomedical Science and Engineering, Missouri University of Science and Technology, Rolla, MO 65409, USA; Center for Single Nanoparticle, Single Cell, and Single Molecule Monitoring, Missouri University of Science and Technology, Rolla, MO 65409, USA. Electronic address: honglan@mst.edu.
Honglan
H
Shi
D001881:Borates / Q000494:pharmacology*; D002460:Cell Line; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005898:Glass / Q000737:chemistry*; D006801:Humans; D007477:Ions; D012212:Rheology; D013052:Spectrometry, X-Ray Emission; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D014961:X-Ray Diffraction
D001881:Borates; D007477:Ions; D042461:Vascular Endothelial Growth Factor A; C009753:fiberglass
Bioactive borate glass nano-/micro-fibers; Copper/zinc (Cu/Zn) doped glass fibers; Dynamic flow culturing module; Vascular endothelial growth factor (VEGF); Wound healing