A panel of microRNA signature in serum for colorectal cancer diagnosis.
Dysregulated expression of specific microRNAs (miRNAs) in serum has been recognised as promising diagnostic biomarkers for colorectal cancer (CRC). In the initial screening phase, a total of 32 differentially expressed miRNAs were selected by quantitative reverse transcription polymerase chain reaction (qRT-PCR) based Exiqon panel with 3 CRC pool samples and 1 normal control (NC) pool. Using qRT-PCR, selected serum miRNAs were further confirmed in training (30 CRC VS. 30 NCs) and testing stages (136 CRC VS. 90 NCs). We identified that serum levels of miR-19a-3p, miR-21-5p and miR-425-5p were significantly higher in patients with CRC than in NCs. The areas under the receiver operating characteristic (ROC) curve of the three-miRNA panel were 0.86, 0.74 and 0.87 for the training, testing and the external validation stages (30 CRC VS. 18 NCs), respectively. Significantly, elevated expression of the three miRNAs was also observed in CRC tissues (n = 24). Furthermore, the expression levels of the three miRNAs were significantly elevated in exosomes from CRC serum samples (n = 10). In conclusion, we identified a serum three-miRNA panel for the diagnosis of CRC.
Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.|Department of Radiation Oncology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
Mingxia
M
Zhu
Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Zebo
Z
Huang
Department of Oncology, The Third Affiliated Hospital of Soochow University, Changzhou 213003, China.
Danxia
D
Zhu
Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Xin
X
Zhou
Department of Respiration, The Affiliated Jiangning Hospital of Nanjing Medical University, Nanjing 210009, China.
Xia
X
Shan
State Key Laboratory of Natural Medicines and Department of Pharmacognosy, China Pharmaceutical University, Nanjing, 210009, China.
Lian-Wen
LW
Qi
Department of Radiation Oncology, Jiangsu Cancer Hospital, Nanjing 210009, China.
Lirong
L
Wu
Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Wenfang
W
Cheng
Department of Radiation Oncology, Jiangsu Cancer Hospital, Nanjing 210009, China.
Jun
J
Zhu
Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Lan
L
Zhang
Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Huo
H
Zhang
Department of Emergency, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Yan
Y
Chen
Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Wei
W
Zhu
Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Tongshan
T
Wang
Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.|Cancer Center of Nanjing Medical University, Nanjing 210029, China.
Ping
P
Liu
D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D012372:ROC Curve; D020133:Reverse Transcriptase Polymerase Chain Reaction
D014408:Biomarkers, Tumor; C549214:MIRN19A microRNA, human; C524940:MIRN21 microRNA, human; C555677:MIRN425 microRNA, human; D035683:MicroRNAs
colorectal cancer; diagnostic biomarker; qRT-PCR; serum microRNA