Novel flexible nerve conduits made of water-based biodegradable polyurethane for peripheral nerve regeneration.
Peripheral nerve conduits were fabricated from biodegradable polyurethane (PU) which was synthesized by a waterborne process. The biodegradable PU was based on poly(ε-caprolactone) diol and polyethylene butylene adipate diol (2:3 molar ratio) as the soft segment. Conduits formed by the freeze-drying process had asymmetric microporous structure. The PU nerve conduits were used to bridge a 10-mm gap in rat sciatic nerve. Nerve regeneration was evaluated by walking track analysis, magnetic resonance imaging (MRI), electrophysiological, and histological analyses. Results demonstrated that after 6 weeks, walking function was recovered by 40%. MR images showed that the transected nerve was reconnected after 3 weeks and the diameter of the regenerated nerve increased from 3 to 6 weeks. The nerve conduction velocity of the regenerated nerve reached 50% of the normal value after 6 weeks. Histological examination revealed that the cross-sectional area of the regenerated nerve at the midconduit was 0.24 mm2after 6 weeks. The efficacy of PU nerve conduits based on functional recovery and histology was superior to that of commercial conduits (Neurotube). The PU nerve conduit developed in this study may be a potential candidate for clinical peripheral nerve tissue engineering. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1383-1392, 2017.
Institute of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan.
Shan-Hui
SH
Hsu
Institute of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan.
Wen-Chi
WC
Chang
Department of Life Science and Institute of Zoology, National Taiwan University, Taipei, Taiwan.
Chen-Tung
CT
Yen
D020341:Absorbable Implants; D000818:Animals; D009416:Nerve Regeneration / Q000187:drug effects*; D059348:Peripheral Nerve Injuries / Q000628:therapy*; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D011140:Polyurethanes / Q000737:chemistry / Q000494:pharmacology; D051381:Rats
D011091:Polyesters; D011140:Polyurethanes; C016240:polycaprolactone
biodegradable polyurethane; magnetic resonance imaging; nerve conduits; peripheral nerve regeneration