Circulating Docosahexaenoic Acid Associates with Insulin-Dependent Skeletal Muscle and Whole Body Glucose Uptake in Older Women Born from Normal Weight Mothers.
BACKGROUND
Obesity among pregnant women is common, and their offspring are predisposed to obesity, insulin resistance, and diabetes. The circulating metabolites that are related to insulin resistance and are associated with this decreased tissue-specific uptake are unknown. Here, we assessed metabolite profiles in elderly women who were either female offspring from obese mothers (OOM) or offspring of lean mothers (OLM). Metabolic changes were tested for associations with metrics for insulin resistance.


METHODS
Thirty-seven elderly women were separated into elderly offspring from obese mothers (OOM;n= 17) and elderly offspring from lean/normal weight mothers (OLM;n= 20) groups. We measured plasma metabolites using proton nuclear magnetic resonance (¹H-NMR) and insulin-dependent tissue-specific glucose uptake in skeletal muscle was assessed. Associations were made between metabolites and glucose uptake.


RESULTS
Compared to the OLM group, we found that the docosahexaenoic acid percentage of the total long-chainn-3 fatty acids (DHA/FA) was significantly lower in OOM (p= 0.015). DHA/FA associated significantly with skeletal muscle glucose uptake (GU) (p= 0.031) and the metabolizable glucose value derived from hyperinsulinemic-euglycemic clamp technique (M-value) in the OLM group only (p= 0.050).


CONCLUSIONS
DHA/FA is associated with insulin-dependent skeletal muscle glucose uptake and this association is significantly weakened in the offspring of obese mothers.
Turku PET Centre, University of Turku, Turku FI-20521, Finland. robert.badeau@mnsttr.com.|Division of Natural Sciences, Department of Health Sciences, Franklin Pierce University, Rindge, NH 03461, USA. robert.badeau@mnsttr.com.
Robert M
RM
Badeau
Turku PET Centre, University of Turku, Turku FI-20521, Finland. mjhonk@utu.fi.
Miikka-Juhani
MJ
Honka
Turku PET Centre, University of Turku, Turku FI-20521, Finland. marco.bucci@utu.fi.
Marco
M
Bucci
Turku PET Centre, University of Turku, Turku FI-20521, Finland. patricia.iozzo@ifc.cnr.it.|Institute of Clinical Physiology, National Research Council, Pisa 56124, Italy. patricia.iozzo@ifc.cnr.it.
Patricia
P
Iozzo
Folkhälsan Research Centre, Helsinki FI-00250, Finland. johan.eriksson@helsinki.fi.|Department of Chronic Disease Prevention, National Institute for Health and Welfare, Helsinki FI-00271, Finland. johan.eriksson@helsinki.fi.|Department of General Practice and Primary Health Care, University of Helsinki and Helsinki University Hospital, Helsinki FI-00014, Finland. johan.eriksson@helsinki.fi.|Vaasa Central Hospital, Vaasa FI-65130, Finland. johan.eriksson@helsinki.fi.
Johan G
JG
Eriksson
Turku PET Centre, University of Turku, Turku FI-20521, Finland. pirjo.nuutila@utu.fi.|Department of Endocrinology, Turku University Hospital, Turku FI-20521, Finland. pirjo.nuutila@utu.fi.
Pirjo
P
Nuutila
D000368:Aged; D004281:Docosahexaenoic Acids / Q000097:blood*; D005260:Female; D005947:Glucose / Q000502:physiology*; D006801:Humans; D056865:Ideal Body Weight; D007328:Insulin / Q000502:physiology; D007333:Insulin Resistance / Q000502:physiology; D008137:Longitudinal Studies; D009035:Mothers; D018482:Muscle, Skeletal / Q000378:metabolism*; D009765:Obesity / Q000097:blood / Q000209:etiology / Q000503:physiopathology; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000097:blood / Q000503:physiopathology; D066244:Proton Magnetic Resonance Spectroscopy
D007328:Insulin; D004281:Docosahexaenoic Acids; D005947:Glucose
22:6 docosahexaenoic acid; insulin-dependent skeletal muscle glucose uptake; offspring of lean mothers; offspring of obese mothers; ω-3 fatty acids