Suspended Manufacture of Biological Structures.
A method for the production of complex cell-laden structures is reported, which allows high-levels of spatial control over mechanical and chemical properties. The potential of this method for producing complicated tissues is demonstrated by manufacturing a complex hard/soft tissue interface and demonstrating that cell phenotype can be maintained over four weeks of culture.
Department of Pharmacy, University of Huddersfield, Queensgate, Huddersfield, HD1 3DH, UK.
Samuel R
SR
Moxon
School of Chemical Engineering, University of Birmingham, Edgbaston, B15 2TT, UK.|Institute of Inflammation and Ageing, MRC Musculoskeletal Ageing Centre, QE Hospital, University of Birmingham, Edgbaston, B15 2WB, UK.
Megan E
ME
Cooke
School of Chemical Engineering, University of Birmingham, Edgbaston, B15 2TT, UK.
Sophie C
SC
Cox
School of Chemical Engineering, University of Birmingham, Edgbaston, B15 2TT, UK.|Royal Orthopaedic Hospital, Bristol Road, Northfield, Birmingham, B31 2AP, UK.
Martyn
M
Snow
Royal Orthopaedic Hospital, Bristol Road, Northfield, Birmingham, B31 2AP, UK.
Lee
L
Jeys
Institute of Inflammation and Ageing, MRC Musculoskeletal Ageing Centre, QE Hospital, University of Birmingham, Edgbaston, B15 2WB, UK.
Simon W
SW
Jones
Department of Pharmacy, University of Huddersfield, Queensgate, Huddersfield, HD1 3DH, UK.
Alan M
AM
Smith
School of Chemical Engineering, University of Birmingham, Edgbaston, B15 2TT, UK.
Liam M
LM
Grover
D002356:Cartilage / Q000166:cytology / Q000502:physiology; D002470:Cell Survival; D019902:Chondrocytes / Q000166:cytology / Q000502:physiology; D018920:Coculture Techniques; D003198:Computer Simulation; D004548:Elasticity; D005269:Femur / Q000166:cytology / Q000502:physiology; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D008422:Materials Testing; D008954:Models, Biological; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology; D011108:Polymers / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D012212:Rheology; D013535:Suspensions / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014783:Viscosity; D055114:X-Ray Microtomography
D020100:Hydrogels; D011108:Polymers; D012333:RNA, Messenger; D013535:Suspensions
additive layer manufacturing; cartilage; hydrogels; osteochondral; tissue engineering