Visual evoked potential in RCS rats with Okayama University-type retinal prosthesis (OUReP™) implantation.
Photoelectric dye-coupled polyethylene film, designated Okayama University type-retinal prosthesis or OUReP™, generates light-evoked surface electric potentials and stimulates neurons. The dye-coupled films or plain films were implanted subretinally in both eyes of 10 Royal College of Surgeons rats with hereditary retinal dystrophy at the age of 6 weeks. Visual evoked potentials in response to monocular flashing light stimuli were recorded from cranially-fixed electrodes, 4 weeks and 8 weeks after the implantation. After the recording, subretinal film implantation was confirmed histologically in 7 eyes with dye-coupled films and 7 eyes with plain films. The recordings from these 7 eyes in each group were used for statistical analysis. The amplitudes of visual evoked potentials in the consecutive time points from 125 to 250 ms after flash were significantly larger in the 7 eyes with dye-coupled film implantation, compared to the 7 eyes with plain film implantation at 8 weeks after the implantation (P < 0.05, repeated-measure ANOVA). The photoelectric dye-coupled polyethylene film, as retinal prosthesis, gave rise to visual evoked potential in response to flashing light.
Ophthalmology, Okayama University Medical School and Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan.
Alamusi
Ophthalmology, Okayama University Medical School and Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan. matsuot@cc.okayama-u.ac.jp.
Toshihiko
http://orcid.org/0000-0001-6570-0030
T
Matsuo
Medical Neurobiology, Okayama University Medical School and Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan.
Osamu
O
Hosoya
Polymer Materials Science, Okayama University Faculty of Engineering and Graduate School of Natural Science and Technology, Okayama, Japan.
Tetsuya
T
Uchida
D000814:Aniline Compounds; D000818:Animals; D004195:Disease Models, Animal; D005074:Evoked Potentials, Visual / Q000502:physiology*; D020959:Polyethylene; D019919:Prosthesis Implantation; D051381:Rats; D058499:Retinal Dystrophies / Q000503:physiopathology* / Q000628:therapy*; D013844:Thiazoles; D057488:Visual Prosthesis
C501867:2-(2-(4-(dibutylamino)phenyl)ethenyl)-3-carboxymethylbenzothiazolium; D000814:Aniline Compounds; D013844:Thiazoles; D020959:Polyethylene
Photoelectric dye; RCS rat; Retinal prosthesis; Retinitis pigmentosa; Visual evoked potential