Resistant maltodextrin or fructooligosaccharides promotes GLP-1 production in male rats fed a high-fat and high-sucrose diet, and partially reduces energy intake and adiposity.
OBJECTIVE
Increasing secretion and production of glucagon-like peptide-1 (GLP-1) by continuous ingestion of certain food components has been expected to prevent glucose intolerance and obesity. In this study, we examined whether a physiological dose (5% weight in diet) of digestion-resistant maltodextrin (RMD) has a GLP-1-promoting effect in rats fed a high-fat and high-sucrose (HFS) diet.


METHODS
Rats were fed a control diet or the HFS (30% fat, 40% sucrose wt/wt) diet supplemented with 5% RMD or fructooligosaccharides (FOS) for 8 weeks or for 8 days in separated experiments. Glucose tolerance, energy intake, plasma and tissue GLP-1 concentrations, and cecal short-chain fatty acids concentrations were assessed.


RESULTS
After 4 weeks of feeding, HFS-fed rats had significantly higher glycemic response to oral glucose than control rats, but rats fed HFS + RMD/FOS did not (approx. 50% reduction vs HFS rats). HFS + RMD/FOS-fed rats had higher GLP-1 responses (~twofold) to oral glucose, than control rats. After 8 weeks, visceral adipose tissue weight was significantly higher in HFS-fed rats than control rats, while HFS + RMD/FOS rats had a trend of reduced gain (~50%) of the tissue weight. GLP-1 contents and luminal propionate concentrations in the large intestine increased (>twofold) by adding RMD/FOS to HFS. Eight days feeding of RMD/FOS-supplemented diets reduced energy intake (~10%) and enhanced cecal GLP-1 production (~twofold), compared to HFS diet.


CONCLUSIONS
The physiological dose of a prebiotic fiber promptly (within 8 days) promotes GLP-1 production in rats fed an obesogenic diet, which would help to prevent excess energy intake and fat accumulation.
Laboratory of Nutritional Biochemistry, Research Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-ku, Sapporo, 060-8589, Japan. hira@chem.agr.hokudai.ac.jp.
Tohru
http://orcid.org/0000-0002-0354-1617
T
Hira
Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
Ryoya
R
Suto
Research & Development, Matsutani Chemical Industry Co., Ltd., Itami, Hyogo, Japan.
Yuka
Y
Kishimoto
Research & Development, Matsutani Chemical Industry Co., Ltd., Itami, Hyogo, Japan.
Sumiko
S
Kanahori
Laboratory of Nutritional Biochemistry, Research Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-ku, Sapporo, 060-8589, Japan.
Hiroshi
H
Hara
D050154:Adiposity; D000818:Animals; D001067:Appetite Depressants / Q000378:metabolism / Q000627:therapeutic use*; D002432:Cecum / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D066273:Diet, Western / Q000009:adverse effects; D004063:Digestion; D064806:Dysbiosis / Q000378:metabolism / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D002149:Energy Intake; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005285:Fermentation; D005766:Gastrointestinal Contents / Q000737:chemistry / Q000382:microbiology; D005786:Gene Expression Regulation; D052216:Glucagon-Like Peptide 1 / Q000819:agonists* / Q000097:blood / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D050152:Intra-Abdominal Fat / Q000473:pathology; D008297:Male; D009765:Obesity / Q000378:metabolism / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D009844:Oligosaccharides / Q000378:metabolism / Q000627:therapeutic use*; D009929:Organ Size; D011134:Polysaccharides / Q000378:metabolism / Q000627:therapeutic use*; D056692:Prebiotics; D017207:Rats, Sprague-Dawley
D001067:Appetite Depressants; D005232:Fatty Acids, Volatile; D009844:Oligosaccharides; D011134:Polysaccharides; D056692:Prebiotics; C116580:fructooligosaccharide; C008315:maltodextrin; D052216:Glucagon-Like Peptide 1
Adiposity; Appetite; Fructooligosaccharides; Glucagon-like peptide-1; High-fat and high-sucrose diet; Resistant maltodextrin