Comparision of Gingival and Umbilical Cord Stem Cells Based on Its Modulus and Neuronal Differentiation.
The availability of Human Umbilical Cord-derived Mesenchymal Stem Cells (HUCMSCs) from a single sex being a major limitation for the utilization of a potential stem cell, it is highly desirable to utilize, an autogenous pluripotent cell with desirable biological and mechanical properties in clinical situations. Comparison of Human Gingival Mesenchymal Stem Cells (HGMSCs) with HUCMSCs demonstrates; MSCs derived from gingiva have higher proliferation rate and higher population doubling time than Umbilical Cord. Unlike HUCMSCs, immunofluorescence studies showed the presence of pluripotency markers OCT-4 and NANOG predominantly in the cytoplasm of HGMSCs which was confirmed by Western blot. The mechanical property, such as modulus of elasticity of HGMSCs, is on par with HUCMSCs, but the surface roughness found to be lesser in HGMSCs, which may suggest HGMSCs greater adhesive property to the extracellular matrix. There is a marginal difference in the neuronal differentiation rate between HGMSCs and HUCMSCs; both the cells expressed positivity for several neuronal lineage markers. Hence, HGMSCs represent an autogenous source of mesenchymal stem cells, which are easy to procure with least morbidity, multipotent in nature with desirable biological, and mechanical properties, probably an ideal candidate for clinical applications. J. Cell. Biochem. 118: 2000-2008, 2017. © 2017 Wiley Periodicals, Inc.
Center for Regenerative Medicine Stem Cell Research, Central Research Facility, Sri Ramachandra University, Chennai, India.
Rajasekaran
R
Subbarayan
Center for Indian Systems of Medicine Quality Assurance and Standardization, Central Research Facility, Sri Ramachandra University, Chennai, India.
Dinesh
D
Murugan Girija
Nano Functional Materials Technology Center, Materials Science Research Center and Department of Physics, Indian Institute of Technology Madras, Chennai, India.
Joynarayan
J
Mukherjee
Nano Functional Materials Technology Center, Materials Science Research Center and Department of Physics, Indian Institute of Technology Madras, Chennai, India.
Sri Ramachandra Rao
SRR
Mamidanna
Faculty of Dental Sciences and Center for Regenerative Medicine and Stem Cell Research, Department of Periodontology and Implantology, Sri Ramachandra University, Chennai, India.
Suresh
S
Ranga Rao
D034961:Antigens, Nuclear / Q000235:genetics* / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D055119:Elastic Modulus; D005312:Fetal Blood / Q000166:cytology* / Q000378:metabolism; D015870:Gene Expression; D005881:Gingiva / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D018625:Microscopy, Atomic Force; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism; D000071317:Nanog Homeobox Protein / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D061251:Primary Cell Culture; D014404:Tubulin / Q000235:genetics / Q000378:metabolism
D034961:Antigens, Nuclear; D015415:Biomarkers; C489788:MAP2 protein, human; D008869:Microtubule-Associated Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D009419:Nerve Tissue Proteins; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; C498585:TUBB3 protein, human; D014404:Tubulin; C506808:neuronal nuclear antigen NeuN, human
HUMAN GINGIVA; MESENCHYMAL STEM CELL; MODULUS; NEURONAL DIFFERENTIATION; UMBILICAL CORD