Application of response surface methodology for air assisted-dispersive liquid- liquid microextraction of deoxynivalenol in rice samples prior to HPLC-DAD analysis and comparison with solid phase extraction cleanup.
A fast, simple, and easy to operate air assisted-dispersive liquid-liquid microextraction (AA-DLLME) for preconcentration and extraction of deoxynivalenol (DON) from rice samples is proposed and compared with solid phase extraction (SPE) cleanup. DON was determined using a high performance liquid chromatography with diode-array detection (HPLC-DAD). AA-DLLME was performed using a glass syringe and dispersion of extractant in the sample solution was achieved with help of air bubbles. Chloroform was used as the extractant solvent. To find out the optimized condition for the proposed method, response surface methodology (RSM) was applied for multivariate optimization of effecting parameters namely volume of extractant, number of extraction, pH, and rate of centrifugation. Under optimized condition the dynamic range of calibration graph was found to be 50-500μgL-1with detection limit of 23.6μgL-1. Both methods were applied for extraction of DON from rice.
Department of Chemistry, Faculty of Sciences, University of Sistan and Baluchestan, Zahedan 98135-674, Iran. Electronic address: rahmani341@hamoon.usb.ac.ir.
Mashaallah
M
Rahmani
Department of Chemistry, Faculty of Sciences, University of Sistan and Baluchestan, Zahedan 98135-674, Iran.
Elham
E
Ghasemi
Department of Chemistry, Faculty of Sciences, University of Sistan and Baluchestan, Zahedan 98135-674, Iran.
Mojtaba
M
Sasani
D000388:Air; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D059627:Liquid Phase Microextraction / Q000379:methods*; D012275:Oryza / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*; D012997:Solvents; D014255:Trichothecenes / Q000032:analysis* / Q000302:isolation & purification*
D012997:Solvents; D014255:Trichothecenes; C007262:deoxynivalenol
Air assisted-dispersive liquid-liquid microextraction; Deoxynivalenol; HPLC-DAD; Response surface methodology; Rice; Solid phase extraction cleanup