Respiratory syncytial virus infection induces a subset of types I and III interferons in human dendritic cells.
Whether respiratory syncytial virus (RSV) induces severe infantile pulmonary disease may depend on viral strain and expression of types I and III interferons (IFNs). These IFNs impact disease severity by inducing expression of many anti-viral IFN-stimulated genes (ISGs). To investigate the impact of RSV strain on IFN and ISG expression, we stimulated human monocyte-derived DCs (MDDCs) with either RSV A2 or Line 19 and measured expression of types I and III IFNs and ISGs. At 24h, A2 elicited higher ISG expression than Line 19. Both strains induced MDDCs to express genes for IFN-β, IFN-α1, IFN-α8, and IFN-λ1-3, but only A2 induced IFN-α2, -α14 and -α21. We then show that IFN-α8 and IFN-α14 most potently induced MDDCs and bronchial epithelial cells (BECs) to express ISGs. Our findings demonstrate that RSV strain may impact patterns of types I and III IFN expression and the magnitude of the ISG response by DCs and BECs.
Laboratory of Immunobiochemistry, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, MD, United States.
Philippa
P
Hillyer
Laboratory of Immunobiochemistry, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, MD, United States.
Viraj P
VP
Mane
Laboratory of Immunobiochemistry, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, MD, United States.
Aaron
A
Chen
Laboratory of Immunobiochemistry, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, MD, United States.
Maria B
MB
Dos Santos
Laboratory of Immunobiochemistry, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, MD, United States.
Lynnsie M
LM
Schramm
Laboratory of Immunobiochemistry, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, MD, United States.
Rachel E
RE
Shepard
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes for Health, Bethesda, MD, United States.
Cindy
C
Luongo
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes for Health, Bethesda, MD, United States.
Cyril
C
Le Nouën
Office of Biostatistics and Epidemiology, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, MD, United States.
Lei
L
Huang
Office of Biostatistics and Epidemiology, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, MD, United States.
Lihan
L
Yan
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes for Health, Bethesda, MD, United States.
Ursula J
UJ
Buchholz
PBL Interferon Source, Piscataway, NJ, United States.
Ronald G
RG
Jubin
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes for Health, Bethesda, MD, United States.
Peter L
PL
Collins
Laboratory of Immunobiochemistry, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, MD, United States. Electronic address: ronald.rabin@fda.hhs.gov.
Ronald L
RL
Rabin
D000328:Adult; D001980:Bronchi / Q000166:cytology; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology* / Q000821:virology; D004847:Epithelial Cells / Q000166:cytology; D006801:Humans; D007249:Inflammation / Q000276:immunology; D016898:Interferon-alpha / Q000378:metabolism*; D016899:Interferon-beta / Q000378:metabolism*; D008168:Lung / Q000276:immunology / Q000473:pathology / Q000821:virology; D020545:Respiratory Mucosa / Q000166:cytology / Q000276:immunology / Q000821:virology; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology* / Q000821:virology; D018113:Respiratory Syncytial Virus, Human / Q000276:immunology* / Q000378:metabolism
D016207:Cytokines; D016898:Interferon-alpha; D016899:Interferon-beta
Bronchial epithelial cell; Dendritic cell; Innate immunity; Interferon; Interferon stimulated gene; Interferon subtype; Respiratory syncytial virus