High-sensitivity immunochromatographic assay for fumonisin B1 based on indirect antibody labeling.
OBJECTIVE
To develop a high-sensitivity immunochromatographic test for fumonisin B1 in plant extracts.


RESULTS
Unlike conventional immunochromatographic tests, this assay is performed in two stages: competitive reaction with free specific antibodies and identifying immune complexes by their interaction with the anti-species antibody-conjugated gold nanoparticles. The use of a new geometry for the test strip membranes and a novel reagent application method ensures the proper order of these stages without additional manipulations. The contact of the ready-to-use test strip with the liquid sample suffices in initiating all stages of the assay and obtaining test results. The developed test was used on corn extracts; its instrumental limit of fumonisin B1 detection was 0.6 ng ml-1at 15 min of assay duration.


CONCLUSIONS
The proposed approach is flexible and can be used for a wide range of low molecular compounds. The use of anti-species antibody-conjugated gold nanoparticles in immunochromatography significantly facilitates the development of test systems by eliminating the need to synthesize and characterize the conjugates with specific antibodies for each new compound to be detected.
A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33 Leninsky prospect, 119071, Moscow, Russia.
Alexandr E
AE
Urusov
A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33 Leninsky prospect, 119071, Moscow, Russia.
Alina V
AV
Petrakova
A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33 Leninsky prospect, 119071, Moscow, Russia.
Milyausha K
MK
Gubaydullina
A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33 Leninsky prospect, 119071, Moscow, Russia.
Anatoly V
AV
Zherdev
A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33 Leninsky prospect, 119071, Moscow, Russia.|Department of Chemical Enzymology, Faculty of Chemistry, M.V. Lomonosov Moscow State University, 1 Leninskie Gory, 119991, Moscow, Russia.
Sergei A
SA
Eremin
School of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, China.
Dezhao
D
Kong
School of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, China.
Liqiang
L
Liu
School of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, China.
Chuanlai
C
Xu
A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33 Leninsky prospect, 119071, Moscow, Russia. dzantiev@inbi.ras.ru.
Boris B
BB
Dzantiev
D055910:Antibodies, Immobilized / Q000737:chemistry / Q000378:metabolism*; D002846:Chromatography, Affinity / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D004867:Equipment Design; D037341:Fumonisins / Q000032:analysis* / Q000378:metabolism*; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry
D055910:Antibodies, Immobilized; D037341:Fumonisins; D010936:Plant Extracts; C056933:fumonisin B1; D006046:Gold
Fumonisin B1; Gold nanoparticles; Immunochromatography; Indirect labeling; Sensitivity enhancement; Test strip