Uterine serpin (SERPINA 14) correlates negatively with cytokine production at the foetal-maternal interface but not in the corpus luteum in pregnant dairy heifers experimentally infected with Neospora caninum.
This study examines gene expression patterns in dairy heifers experimentally infected with N. caninum during on Day 110 of pregnancy with live foetuses at euthanasia, 42 days later. The study population was constituted of four non-infected controls and three infected dams. Gene expression was determined on gamma interferon (IFNγ), (Th1 pro-inflammatory cytokine), interleukin-4 (IL4) (Th2 pro-gestation cytokine) or interleukin-10 (IL10) (T regulatory cytokine) and the serine peptidase inhibitor SERPINA14 in intercaruncular, placental, uterine lymph node (UTLN) and luteal tissue samples. Intercaruncular SERPINA14 expression was negatively correlated with IFNγ expression in cotyledon samples and with IL4 expression in UTLN. No relationships were detected between cytokine gene expression at the foetal-maternal interface and SERPINA14 expression in the luteal samples. Our findings suggest that gene expression of the uterine serpin SERPINA14 correlates negatively with the expression of Th1 and Th2 cytokines at the foetal-maternal interface but not in the corpus luteum.
Department of Animal Science, University of Lleida, Lleida, Spain.|Agrotecnio Centre, University of Lleida, Lleida, Spain.
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B
Serrano-Pérez
Centre de Recerca en Sanitat Animal - Institut de Recerca i Tecnologia Agroalimentàries, Universitat Autònoma de Barcelona, Barcelona, Spain.|Departament de Sanitat i Anatomia Animals, Universitat Autònoma de Barcelona, Barcelona, Spain.
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S
Almería
Department of Animal Science, University of Lleida, Lleida, Spain.
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R
Mur-Novales
Department of Animal Science, University of Lleida, Lleida, Spain.|Agrotecnio Centre, University of Lleida, Lleida, Spain.
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I
López-Helguera
Department of Animal Science, University of Lleida, Lleida, Spain.|Agrotecnio Centre, University of Lleida, Lleida, Spain.
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I
Garcia-Ispierto
Instituto Agroalimentario de Aragón - IA2 - (CITA-Universidad de Zaragoza), Zaragoza, Spain.
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JL
Alabart
Centre de Recerca en Sanitat Animal - Institut de Recerca i Tecnologia Agroalimentàries, Universitat Autònoma de Barcelona, Barcelona, Spain.|Departament de Sanitat i Anatomia Animals, Universitat Autònoma de Barcelona, Barcelona, Spain.
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L
Darwich
Agrotecnio Centre, University of Lleida, Lleida, Spain.|Transfer in Bovine Reproduction SLu, Barbastro, Spain.
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http://orcid.org/0000-0002-1413-3862
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López-Gatius
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism / Q000469:parasitology*; D003048:Coccidiosis / Q000469:parasitology / Q000662:veterinary*; D003338:Corpus Luteum / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D005333:Fetus; D005786:Gene Expression Regulation / Q000502:physiology; D008431:Maternal-Fetal Exchange; D018688:Neospora; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000662:veterinary; D015843:Serpins / Q000235:genetics / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism
D016207:Cytokines; D015843:Serpins
bovine neosporosis; maternal immune response; placenta; pregnancy