Enhancement of curcumin wound healing ability by complexation with 2-hydroxypropyl-γ-cyclodextrin in sacran hydrogel film.
Curcumin is one of promising agents to accelerate the wound-healing process. However, the efficacy of curcumin is limited due to its poor water solubility and stability. To enhance the properties of curcumin, 2-hydroxypropyl-γ-cyclodextrin (HP-γ-CyD) can be used through complexation. Recently, we revealed that sacran has the potential to form a hydrogel film (HGF) as a wound dressing material. Therefore, in the present study, we investigated the wound healing ability of curcumin/HP-γ-CyD (Cur/HP-γ-CyD) complex in sacran-based HGF (Sac-HGF). We prepared the Cur/HP-γ-CyD complex in Sac-HGF without surface roughness. Additionally, the amorphous form in the Cur/HP-γ-CyD complex in Sac-HGF were observed. In contrast, the curcumin in Sac-HGF and curcumin/HP-γ-CyD physical mixture in Sac-HGF formed inhomogeneous films due to crystallization of curcumin. Furthermore, HP-γ-CyD played an important role to increase the elastic modulus of the Sac-HGF with high re-swelling ability. The Cur/HP-γ-CyD complex in Sac-HGF maintained antioxidant properties of curcumin. Curcumin was gradually released from the HP-γ-CyD complex in Sac-HGF. Notably, the Cur/HP-γ-CyD complex in Sac-HGF provided the highest wound healing ability in hairless mice. These results suggest that the Cur/HP-γ-CyD complex in Sac-HGF has the potential for use as a new transdermal therapeutic system to promote the wound-healing process.
Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto, 862-0973, Japan; Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, 45363, Indonesia.
Nasrul
N
Wathoni
Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto, 862-0973, Japan.
Keiichi
K
Motoyama
Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto, 862-0973, Japan.
Taishi
T
Higashi
Japan Advanced Institute of Science and Technology (JAIST), 1-1 Asahidai, Nomi-shi, Ishikawa, 923-1292, Japan.
Maiko
M
Okajima
Japan Advanced Institute of Science and Technology (JAIST), 1-1 Asahidai, Nomi-shi, Ishikawa, 923-1292, Japan.
Tatsuo
T
Kaneko
Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto, 862-0973, Japan; Program for Leading Graduate Schools "Health Life Science: Interdisciplinary and Glocal Oriented (HIGO) Program", Kumamoto University, Japan. Electronic address: arimah@gpo.kumamoto-u.ac.jp.
Hidetoshi
H
Arima
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D003474:Curcumin / Q000737:chemistry* / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D004355:Drug Stability; D008756:Methylgalactosides / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D011134:Polysaccharides / Q000737:chemistry*; D012995:Solubility; D013329:Structure-Activity Relationship; D014867:Water / Q000737:chemistry; D014945:Wound Healing / Q000187:drug effects*; D047408:gamma-Cyclodextrins / Q000737:chemistry* / Q000633:toxicity
D000975:Antioxidants; D004337:Drug Carriers; D008756:Methylgalactosides; D011134:Polysaccharides; D047408:gamma-Cyclodextrins; C109801:hydrogel film; C479659:hydroxypropyl-gamma-cyclodextrin; C571099:sacran; D014867:Water; D003474:Curcumin
Curcumin; Hydroxypropyl-γ-cyclodextrin; Sacran hydrogel film