Assessment of the Usefulness of Multiplex Real-Time PCR Tests in the Diagnostic and Therapeutic Process of Pneumonia in Hospitalized Children: A Single-Center Experience.
The aim of the study was assessment of the usefulness of multiplex real-time PCR tests in the diagnostic and therapeutic process in children hospitalized due to pneumonia and burdened with comorbidities.Methods. The study group included 97 children hospitalized due to pneumonia at the Karol Jonscher Teaching Hospital in Poznań, in whom multiplex real-time PCR tests (FTD respiratory pathogens 33; fast-track diagnostics) were used.Results. Positive test results of the test were achieved in 74 patients (76.3%). The average age in the group was 56 months. Viruses were detected in 61 samples (82% of all positive results); bacterial factors were found in 29 samples (39% of all positive results). The presence of comorbidities was established in 90 children (92.78%). On the basis of the obtained results, 5 groups of patients were established: viral etiology of infection, 34 patients; bacterial etiology, 7 patients; mixed etiology, 23 patients; pneumocystis, 9 patients; and no etiology diagnosed, 24 patients.Conclusions. Our analysis demonstrated that the participation of viruses in causing severe lung infections is significant in children with comorbidities. Multiplex real-time PCR tests proved to be more useful in establishing the etiology of pneumonia in hospitalized children than the traditional microbiological examinations.
Department of Family Medicine, University of Medical Sciences, Poznan, Poland.
Ewelina
0000-0001-7443-0749
E
Gowin
Department of Paediatric Anaesthesiology and Intensive Therapy, University of Medical Sciences, Poznan, Poland.
Alicja
A
Bartkowska-Śniatkowska
Department of Paediatric Radiology, University of Medical Sciences, Poznan, Poland.
Katarzyna
K
Jończyk-Potoczna
Department of Medical Diagnostics, University of Medical Sciences, Dobra 38, Poznan, Poland.
Joanna
J
Wysocka-Leszczyńska
Department of Paediatric Cardiology, University of Medical Sciences, Poznan, Poland.
Waldemar
W
Bobkowski
Department of Paediatric Diabetology, University of Medical Sciences, Poznan, Poland.
Piotr
P
Fichna
Department of Paediatric Oncology, Hematology and Bone Marrow Transplantation, University of Medical Sciences, Poznan, Poland.
Paulina
P
Sobkowiak
Department of Infectious Diseases, University of Medical Sciences, Poznan, Poland.
Katarzyna
K
Mazur-Melewska
Department of Paediatric Pulmonology, University of Medical Sciences, Poznan, Poland.
Anna
A
Bręborowicz
Department of Health Promotion, University of Medical Sciences, Poznan, Poland.
Jacek
J
Wysocki
Department of Medical Diagnostics, University of Medical Sciences, Dobra 38, Poznan, Poland; Department of Paediatric Oncology, Hematology and Bone Marrow Transplantation, University of Medical Sciences, Poznan, Poland; Department of Molecular Pathology, Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland.
Danuta
D
Januszkiewicz-Lewandowska
D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D004279:DNA, Viral / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D060885:Multiplex Polymerase Chain Reaction; D011014:Pneumonia / Q000097:blood* / Q000235:genetics* / Q000382:microbiology / Q000821:virology; D060888:Real-Time Polymerase Chain Reaction; D014780:Viruses / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity
D004269:DNA, Bacterial; D004279:DNA, Viral