Relationship between insulin resistance, hs-CRP, and body fat and serum osteoprotegerin/RANKL in prediabetic patients.
BACKGROUND
Receptor activator of nuclear factor-κB ligand (RANKL) and osteoprotegerin (OPN) are soluble members of the tumor necrosis factor superfamily. Growing evidence suggest that there is link between inflammation, insulin resistance and OPG, soluble RANKL (sRANKL). We aimed to ascertain whether OPG and sRANKL levels are altered in prediabetic subjects and there is association between OPG/sRANKL and metabolic parameters.


METHODS
Forty prediabetic subjects and 40 age- and BMI-matched controls were recruited for this cross-sectional study. Circulating OPG, sRANKL were measured using ELISA. Anthropometric and metabolic parameters were also determined.


RESULTS
Circulating sRANKL (97.74±17.67 vs. 55.00±11.19 pg/mL, P=0.010) and OPG (261.54±74.55 vs. 159.23±52.91 pg/mL, P=0.020) levels were found to be significantly higher in diabetic subjects compared with control subjects. There was a positive correlation between sRANKL and OPG. sRANKL also positively correlated with BMI, insulin resistance marker HOMA-IR, inflammatory marker hs-CRP. Logistic regression analyses revealed that the odds ratio was increased for prediabetes in subjects with having elevated sRANKL levels.


CONCLUSIONS
Increased sRANKL and OPG levels were associated with prediabetic subjects. sRANKL and OPG may play a role in the pathogenesis of diabetes as well as metabolic disturbance.
Department of Internal Medicine, Izmir Bozyaka Training and Research Hospital, Bozyaka, Izmir, Turkey - oktaybilgir@gmail.com.
Oktay
O
Bilgir
Department of Internal Medicine, Izmir Bozyaka Training and Research Hospital, Bozyaka, Izmir, Turkey.
Mehmet
M
Yavuz
Department of Allergy and Immunology, Medical School, Katip Celebi University, Ataturk Training and Research Hospital, Izmir, Turkey.
Ferda
F
Bilgir
Department of Internal Medicine, Izmir Bozyaka Training and Research Hospital, Bozyaka, Izmir, Turkey.
Ozden Y
OY
Akan
Department of Family Medicine, Izmir Bozyaka Training and Research Hospital, Bozyaka, Izmir, Turkey.
Aslı G
AG
Bayindir
Division of Endocrinology and Metabolism, Department of Internal Medicine, Izmir Bozyaka Training and Research Hospital, Bozyaka, Izmir, Turkey.
Mehmet
M
Calan
Department of Biochemistry, Izmir Bozyaka Training and Research Hospital, Bozyaka, Izmir, Turkey.
Giray
G
Bozkaya
Department of Internal Medicine, Izmir Bozyaka Training and Research Hospital, Bozyaka, Izmir, Turkey.
Arif
A
Yuksel
D000273:Adipose Tissue; D000328:Adult; D000368:Aged; D002097:C-Reactive Protein / Q000235:genetics*; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D053244:Osteoprotegerin / Q000097:blood / Q000235:genetics*; D011236:Prediabetic State / Q000235:genetics*; D053245:RANK Ligand / Q000097:blood / Q000235:genetics*
D053244:Osteoprotegerin; D053245:RANK Ligand; C506030:TNFRSF11B protein, human; C505688:TNFSF11 protein, human; D002097:C-Reactive Protein