Fluorescence In Situ Hybridization with the UroVysion Kit for the Detection of Biliary Cancer in Chinese Patients.
BACKGROUND
Conventional biliary brush via ERCP has low clinical detection for biliary malignancy. Therefore, new approaches are needed to facilitate diagnosis. We therefore explored the application of fluorescent in situ hybrization (FISH) using a UroVysion kit for the detection of malignancy in the bile duct.


METHODS
Genetic alterations of target chromosomes such as aneuploidy in Chinese biliary cancer cell lines and tissues were measured using a UroVysion kit. The diagnostic value of the FISH assay was assessed by probing 27 brush samples of biliary cytology and control routine cytology (RC) samples. The gold standard was established by the pathology or clinical outcomes at the 12-month follow-up.


RESULTS
Aneuploidy is commonly found in cell lines and tissues of biliary cancers, but not in normal cells or tissues. Here we probed for aneuploidy in clinical biliary brush specimens obtained by ERCP using FISH and a UroVysion kit. The sensitivity, specificity, and positive and negative predictive values for biliary malignancy were found to be 50%, 100%, 100% and 31.3%, respectively. The sensitivity, specificity, and positive and negative predictive values by RC were found to be 22.7%, 100%, 100% and 22.7%, respectively. In combination with RC, FISH increased the diagnostic sensitivity to 63.6% although this difference was not found to be statistically significant.


CONCLUSIONS
Aneuploidy is frequently present in bile duct carcinomas. Here we found that the FISH assay is useful for the detection of Chinese biliary cancers.
Xi
X
Wu
Xuan
X
Zeng
Aiming
A
Yang
Fang
F
Yao
Tao
T
Guo
Dongsheng
D
Wu
Shafei
S
Wu
Hongmei
H
Qiu
Weixun
W
Zhou
Zhen
Z
Huo
Xinghua
X
Lu
Jiaming
J
Qian
D000782:Aneuploidy; D001661:Biliary Tract Neoplasms / Q000235:genetics* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000235:genetics*; D045744:Cell Line, Tumor; D002681:China; D002760:Cholangiopancreatography, Endoscopic Retrograde; D002877:Chromosomes, Human; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D011237:Predictive Value of Tests; D011379:Prognosis; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D013997:Time Factors
D014408:Biomarkers, Tumor; D011933:Reagent Kits, Diagnostic