17β-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats.
The aim of this study was to investigate the in vivo effects of 17β-estradiol (E2) on myocardial metabolism and inducible nitric oxide synthase (iNOS) expression/activity in obese rats. Male Wistar rats were fed with a normal or a high fat (HF) diet (42% fat) for 10 weeks. Half of the HF fed rats were treated with a single dose of E2while the other half were placebo-treated. 24 h after treatment animals were sacrificed. E2reduced cardiac free fatty acid (FFA) (p < 0.05), L-arginine (p < 0.01), iNOS mRNA (p < 0.01), and protein (p < 0.05) levels and translocation of the FFA transporter (CD36) (p < 0.01) to the plasma membrane (PM) in HF fed rats. In contrast, Akt phosphorylation at Thr308(p < 0.05) and translocation of the glucose transporter GLUT4 (p < 0.05) to the PM increased after E2treatment in HF rats. Our results indicate that E2acts via the PI3K/Akt signalling pathway to partially protect myocardial metabolism by attenuating the detrimental effects of increased iNOS expression/activity in HF fed rats.
Institute of Nuclear Sciences Vinca, University of Belgrade, Laboratory of Radiobiology and Molecular Genetics, Mike Petrovica Alasa 12-14, 11000 Belgrade, Serbia. Electronic address: sonjazafirovic@hotmail.com.
Sonja
S
Zafirovic
Institute of Nuclear Sciences Vinca, University of Belgrade, Laboratory of Radiobiology and Molecular Genetics, Mike Petrovica Alasa 12-14, 11000 Belgrade, Serbia. Electronic address: obradovicmilan@hotmail.com.
Milan
M
Obradovic
Institute of Nuclear Sciences Vinca, University of Belgrade, Laboratory of Radiobiology and Molecular Genetics, Mike Petrovica Alasa 12-14, 11000 Belgrade, Serbia. Electronic address: emma_crash@yahoo.com.
Emina
E
Sudar-Milovanovic
Institute of Nuclear Sciences Vinca, University of Belgrade, Laboratory of Radiobiology and Molecular Genetics, Mike Petrovica Alasa 12-14, 11000 Belgrade, Serbia. Electronic address: jovsale@gmail.com.
Aleksandra
A
Jovanovic
Institute of Nuclear Sciences Vinca, University of Belgrade, Laboratory of Radiobiology and Molecular Genetics, Mike Petrovica Alasa 12-14, 11000 Belgrade, Serbia. Electronic address: julijana1008@gmail.com.
Julijana
J
Stanimirovic
School of Medicine, University of St Andrews, North Haugh, St Andrews, KY16 9TF, United Kingdom. Electronic address: ajs21@st-andrews.ac.uk.
Alan J
AJ
Stewart
School of Medicine, University of St Andrews, North Haugh, St Andrews, KY16 9TF, United Kingdom. Electronic address: sjp24@st-andrews.ac.uk.
Samantha J
SJ
Pitt
Institute of Nuclear Sciences Vinca, University of Belgrade, Laboratory of Radiobiology and Molecular Genetics, Mike Petrovica Alasa 12-14, 11000 Belgrade, Serbia. Electronic address: isenovic@yahoo.com.
Esma R
ER
Isenovic
D000818:Animals; D059305:Diet, High-Fat; D004958:Estradiol / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism; D005247:Feeding Behavior; D005947:Glucose / Q000378:metabolism*; D050356:Lipid Metabolism / Q000187:drug effects*; D008297:Male; D026901:Membrane Transport Proteins / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D017208:Rats, Wistar; D011960:Receptors, Estrogen / Q000378:metabolism
D005227:Fatty Acids; D026901:Membrane Transport Proteins; D009566:Nitrates; D009573:Nitrites; D020011:Protective Agents; D011960:Receptors, Estrogen; D009569:Nitric Oxide; D004958:Estradiol; D052247:Nitric Oxide Synthase Type II; D051057:Proto-Oncogene Proteins c-akt; D005947:Glucose
Cardiovascular diseases; Estradiol; Inducible nitric oxide synthase; Myocardial metabolism; Obesity