Commiphora leptophloeosPhytochemical and Antimicrobial Characterization.
Commiphora leptophloeosis a plant specie usually known for its medicinal purposes in local communities in Northeast Brazil. In order to evaluate its therapeutic potential, we aimed to determine the phytochemical and antimicrobial properties ofC. leptophloeosextracts. Thin Layer Chromatography (TLC) was able to detect the presence of phenolic compounds, flavonoids and reducing sugars. Three phenolic compounds were identified by HPLC and described as Gallic, Chlorogenic and Protocatechuic acids. On the other hand, H1NMR analysis revealed the presence of hinokinin, a bioactive lignan further characterized in the present work. The minimum inhibitory concentration (MIC) values for hinokinin ranged from 0.0485 to 3.125 mg/mL in differentS. aureusclinical isolates and showed a bactericidal activity against MRSA isolated from blood (MMC 0.40 mg/mL) and postoperative secretion (MMC = 3.125 mg/mL).C. leptophloeosextracts also showed antimicrobial activity against Mycobacterium species such asM. smegmatis(MIC = 12.5 mg/mL) andM. tuberculosis(MIC = 52 mg/mL). Additionally, we determined the toxicity ofC. leptophloeosbyin vitroHC50tests with hemolytic activity detected of 313 ± 0.5 μg/mL. Our results showed thatC. leptophloeospossesses inhibitory properties against MRSA as well as several other clinically important microorganisms. Furthermore, the present work is the first report of the presence of hinokinin in Commiphora genus.
Department of Genetics, Federal University of PernambucoRecife, Brazil; Laboratory of Immunopathology Keizo Asami, Federal University of PernambucoRecife, Brazil.
Jorge J
JJ
de Souza Pereira
Laboratory of Glycoproteins, Department of Biochemistry, Federal University of Pernambuco Recife, Brazil.
Aline de P C
AP
Pereira
Laboratory of Glycoproteins, Department of Biochemistry, Federal University of Pernambuco Recife, Brazil.
Jannyson J B
JJ
Jandú
Department of Fundamental Chemistry, Federal University of Pernambuco Recife, Brazil.
Josinete A
JA
da Paz
Department of Genetics, Federal University of PernambucoRecife, Brazil; Laboratory of Immunopathology Keizo Asami, Federal University of PernambucoRecife, Brazil.
Sergio
S
Crovella
Laboratory of Glycoproteins, Department of Biochemistry, Federal University of Pernambuco Recife, Brazil.
Maria T
MT
Dos Santos Correia
Department of Genetics, Federal University of PernambucoRecife, Brazil; Laboratory of Immunopathology Keizo Asami, Federal University of PernambucoRecife, Brazil.
Jaqueline
J
de Azevêdo Silva
Mycobacterium tuberculosis; S. aureus; antibacterial agents; drug discovery; hinokinin; multidrug resistance; plant-derived products