Analysis ofGrapevine rupestris stem pitting-associated virusin Slovakia Reveals Differences in Intra-Host Population Diversity and Naturally Occurring Recombination Events.
Grapevine rupestris stem pitting-associated virus(GRSPaV) is a worldwide-distributed pathogen in grapevines with a high genetic variability. Our study revealed differences in the complexity of GRSPaV population in a single host. A single-variant GRSPaV infection was detected from the SK30 grapevine plant. On the contrary, SK704 grapevine was infected by three different GRSPaV variants. Variant-specific RT-PCR detection protocols have been developed in this work to study distribution of the three different variants in the same plant during the season. This study showed their randomized distribution in the infected SK704 grapevine plant. Comparative analysis of fulllength genome sequences of four Slovak GRSPaV isolates determined in this work and 14 database sequences showed that population of the virus cluster into four major phylogenetic lineages. Moreover, our analyses suggest that genetic recombination along with point mutations could play a significant role in shaping evolutionary history of GRSPaV and contributed to its extant genetic diversification.
Institute of Virology, Biomedical Research Centre, Slovak Academy of Sciences, Bratislava 84505, Slovakia.
Miroslav
M
Glasa
Institute of Virology, Biomedical Research Centre, Slovak Academy of Sciences, Bratislava 84505, Slovakia.
Lukáš
L
Predajňa
Comenius University Science Park, Comenius University, Bratislava 84216, Slovakia.
Katarína
K
Šoltys
Institute of Virology, Biomedical Research Centre, Slovak Academy of Sciences, Bratislava 84505, Slovakia.
Nina
N
Sihelská
Institute of Virology, Biomedical Research Centre, Slovak Academy of Sciences, Bratislava 84505, Slovakia.
Alžbeta
A
Nagyová
DLR Rheinpfalz, Institute of Plant Protection, Neustadt an der Weinstrasse 67435, Germany.
Thierry
T
Wetzel
Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, MS 39762, USA.
Sead
S
Sabanadzovic
Foveavirus; Vitis vinifera; detection; mixed infection; recombination