Development and validation of a reversed-phase HPLC method for licarbazepine monitoring in serum of patients under oxcarbazepine treatment.
Licarbazepine is the pharmacologically active metabolite of oxcarbazepine, a drug indicated for the treatment of partial seizures and bipolar disorders. Several HPLC methods have been developed thus far but there is lack of control for interferences from antipsychotic drugs. The aim of the present study was to develop a simple, low-cost and reliable HPLC-UV method for the determination of licarbazepine in human serum in the presence of co-administered antiepileptic, antipsychotic and commonly prescribed drugs. Sample preparation consisted of a single protein precipitation step with methanol. Separation lasted ~9 min on a reversed-phase C18column using a mobile phase composed of 50 mm sodium-dihydrogen-phosphate-monohydrate/acetonitrile (70:30, v/v) delivered isocratically at 0.9 mL/min and 30°C. Wavelength was 210 nm and calibration curve was linear with r20.998 over the range 0.2-50.0 μg/mL. Coefficient of variation was <5.03% and bias <-4.92%. Recovery ranged from 99.49 to 104.52% and the limit of detection was 0.0182 μg/mL. No interferences from the matrix or from antiepileptic, antipsychotic and commonly prescribed drugs were observed. The method was applied to serum samples of patients under oxcarbazepine treatment and proved to be a useful tool for the therapeutic drug monitoring of licarbazepine during monotherapy or adjunctive treatment of seizures or affective disorders.
Laboratory of Pharmacology, School of Medicine, University of Thessaly, Larissa, Greece.
Elias
http://orcid.org/0000-0001-8647-4474
E
Begas
Laboratory of Chemistry, School of Medicine, University of Thessaly, Larissa, Greece.
Andreas
A
Tsakalof
Department of Neurology, University Hospital of Larissa, School of Medicine, University of Thessaly, Larissa, Greece.
Efthimios
E
Dardiotis
Laboratory of Pharmacology, School of Medicine, University of Thessaly, Larissa, Greece.
Georgios Emmanouil
GE
Vatidis
Laboratory of Pharmacology, School of Medicine, University of Thessaly, Larissa, Greece.
Evangelos
E
Kouvaras
Laboratory of Pharmacology, School of Medicine, University of Thessaly, Larissa, Greece.
Eftihia Konstadinos
EK
Asprodini
D000927:Anticonvulsants / Q000097:blood*; D002220:Carbamazepine / Q000031:analogs & derivatives* / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D016903:Drug Monitoring / Q000379:methods; D004827:Epilepsy; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D000078330:Oxcarbazepine; D015203:Reproducibility of Results
D000927:Anticonvulsants; D002220:Carbamazepine; D000078330:Oxcarbazepine
HPLC; affective disorders; epilepsy; licarbazepine; therapeutic drug monitoring