Microbacterium zeae sp. nov., an endophytic bacterium isolated from maize stem.
A novel Gram-stain positive, aerobic, non-motile, non-spore-forming and rod-shaped strain designated 1204Twas isolated from surface-sterilised stem tissue of maize planted in Fangshan District of Beijing, People's Republic of China. A polyphasic taxonomic study was performed on the new isolate. On the basis of 16S rRNA gene sequence similarity studies, this isolate belongs to the genus Microbacterium. High levels of 16S rRNA gene sequence similarity were found between strain 1204Tand Microbacterium enclense NIO-1002T(98.8%) and Microbacterium proteolyticum RZ36T(98.4%) respectively. However, the DNA-DNA hybridization values between strain 1204Tand its closely related species M. proteolyticum DSM 27100Tand M. enclense DSM 25125Twere 53.9 ± 1.6 and 20.9 ± 1.5% respectively. The DNA G+C content of strain 1204Twas determined to be 68.0 mol%. The major fatty acids were found to consist of anteiso-C15:0(37.6%), iso-C16:0(28.6%) and anteiso-C17:0(16.6%). The predominant menaquinone was MK-11 and the polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid and an unidentified lipid. The results of physiological and biochemical tests and minor differences in the fatty acid profiles allowed a clear phenotypic differentiation of strain 1204Tfrom the closely related species in the genus Microbacterium. Thus, it was concluded that strain 1204Trepresents a novel species within the genus Microbacterium, for which the name Microbacterium zeae sp. nov. is proposed, with the type strain 1204T(= CGMCC 1.15289 = DSM 100750).
Beijing Agro- Biotechnology Research Center, Beijing Academy of Agriculture and Forestry/Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing, 100097, People's Republic of China.
Jun-Lian
JL
Gao
State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, People's Republic of China.
Pengbo
P
Sun
Beijing Agro- Biotechnology Research Center, Beijing Academy of Agriculture and Forestry/Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing, 100097, People's Republic of China.
Xu-Ming
XM
Wang
Key Laboratory of Microbial Resources, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.
Fan-Yang
FY
Lv
Key Laboratory of Microbial Resources, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China. sunjianguang@caas.cn.
Jian-Guang
JG
Sun
D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D060026:Endophytes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D005227:Fatty Acids / Q000032:analysis; D006017:Glycolipids / Q000032:analysis; D006801:Humans; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D018547:Plant Stems / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000032:analysis; D003313:Zea mays / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2
Endophytes; Maize; Microbacterium