A Culture-Independent Approach to Enrich Endophytic Bacterial Cells from Sugarcane Stems for Community Characterization.
Bacterial endophytes constitute a very diverse community and they confer important benefits which help to improve agricultural yield. Some of these benefits remain underexplored or little understood, mainly due to the bottlenecks associated with the plant feature, a low number of endophytic bacterial cells in relation to the plant, and difficulties in accessing these bacteria using cultivation-independent methods. Enriching endophytic bacterial cells from plant tissues, based on a non-biased, cultivation-independent physical enrichment method, may help to circumvent those problems, especially in the case of sugarcane stems, which have a high degree of interfering factors, such as polysaccharides, phenolic compounds, nucleases, and fibers. In the present study, an enrichment approach for endophytic bacterial cells from sugarcane lower stems is described. The results demonstrate that the enriched bacterial cells are suitable for endophytic community characterization. A community analysis revealed the presence of previously well-described but also novel endophytic bacteria in sugarcane tissues which may exert functions such as plant growth promotion and biological control, with a predominance of the Proteobacterial phylum, but also Actinobacteria, Bacteroidetes, and Firmicutes, among others. In addition, by comparing the present and literature data, it was possible to list the most frequently detected bacterial endophyte genera in sugarcane tissues. The presented enrichment approach paves the way for improved future research toward the assessment of endophytic bacterial community in sugarcane and other biofuel crops.
Pró-Reitoria de Pesquisa e Pós-Graduação, Universidade Federal Rural do Rio de Janeiro, Rodovia BR 465, Km 7, Seropédica, RJ, CEP 23897-000, Brazil.|Embrapa Agrobiologia, Rodovia BR 465, Km 7, Seropédica, RJ, CEP 23891-000, Brazil.
Carlos M
CM
Dos-Santos
Embrapa Agrobiologia, Rodovia BR 465, Km 7, Seropédica, RJ, CEP 23891-000, Brazil.|Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Rodovia BR 465, Km 7, Seropédica, RJ, CEP 23891-000, Brazil.
Daniel G
DG
de Souza
Departamento de Bioquímica e Biologia Molecular, Setor de Ciências Biológicas, Universidade Federal do Paraná, Centro Politécnico, Jardim das Américas, Curitiba, PR, CEP 81531-980, Brazil.
Eduardo
E
Balsanelli
Departamento de Bioquímica e Biologia Molecular, Setor de Ciências Biológicas, Universidade Federal do Paraná, Centro Politécnico, Jardim das Américas, Curitiba, PR, CEP 81531-980, Brazil.
Leonardo Magalhães
LM
Cruz
Departamento de Bioquímica e Biologia Molecular, Setor de Ciências Biológicas, Universidade Federal do Paraná, Centro Politécnico, Jardim das Américas, Curitiba, PR, CEP 81531-980, Brazil.
Emanuel M
EM
de Souza
Embrapa Agrobiologia, Rodovia BR 465, Km 7, Seropédica, RJ, CEP 23891-000, Brazil.
José I
JI
Baldani
Embrapa Agrobiologia, Rodovia BR 465, Km 7, Seropédica, RJ, CEP 23891-000, Brazil. stefan.schwab@embrapa.br.
Stefan
0000-0001-9058-0632
S
Schwab
D001419:Bacteria / Q000145:classification*; D001431:Bacteriological Techniques; D060026:Endophytes / Q000145:classification; D010802:Phylogeny; D031786:Saccharum / Q000382:microbiology*
Bacterial endophytes; Cell enrichment; Culture-independent approaches; Sugarcane