An Electrochemical Gas Biosensor Based on Enzymes Immobilized on Chromatography Paper for Ethanol Vapor Detection.
This paper presents a novel method of fabricating an enzymatic biosensor for breath analysis using chromatography paper as enzyme supporting layer and a liquid phase layer on top of screen printed carbon electrodes. We evaluated the performance with ethanol vapor being one of the breathing ingredients. The experimental results show that our sensor is able to measure the concentration of ethanol vapor within the range of 50 to 500 ppm. These results suggest the ability of detecting breath ethanol, and it can possibly be applied as a generic vapor biosensor to a wide range of diseases.
Department of Electrical and Electronic Engineering, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan. t.kuretake@gmail.com.
Tatsumi
T
Kuretake
Department of Electrical and Electronic Engineering, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan. re0039ke@ed.ritsumei.ac.jp.
Shogo
S
Kawahara
Department of Electrical and Electronic Engineering, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan. re0064vp@ed.ritsumei.ac.jp.
Masanobu
M
Motooka
Department of Electrical and Electronic Engineering, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan. suno@fc.ritsumei.ac.jp.
Shigeyasu
S
Uno
D015374:Biosensing Techniques; D002845:Chromatography; D004566:Electrodes; D004800:Enzymes, Immobilized; D000431:Ethanol; D005740:Gases
D004800:Enzymes, Immobilized; D005740:Gases; D000431:Ethanol
chromatography paper-based sensor; electrochemical sensor; gas sensor