Mouse intestinal niche cells express a distinct α1,2-fucosylated glycan recognized by a lectin from Burkholderia cenocepacia.
In this study, we examined the distribution of fucosylated glycans in mouse intestines using a lectin, BC2LCN (N-terminal domain of the lectin BC2L-C from Burkholderia cenocepacia), as a probe. BC2LCN is specific for glycans with a terminal Fucα1,2Galβ1,3-motif and it is a useful marker for discriminating the undifferentiated status of human induced/embryonic stem cells. Apparent BC2LCN reactivity was detected in the secretory granules of goblet cells in the ileum but not those in the colon. We also found distinctive reactivity in the crypt bottom, which is known as the stem cell zone, of the colon and the ileum. Other lectins for fucosylated glycans, including Ulex europaeus agglutinin-I, Pholiota squarrosa lectin and Aleuria aurantia lectin, did not exhibit similar reactivity in the crypt bottom. Remarkably, BC2LCN-positive epithelial cells could be labeled with a niche cell marker, c-Kit/CD117. Overall, our results indicate that intestinal niche cells express distinct fucosylated glycans recognized by BC2LCN. Increasing evidence suggests that the self-renewal and proliferation of stem cells depend on specific signals derived from niche cells. Our results highlight novel molecular properties of intestinal niche cells in terms of their glycosylation, which may help to understand the regulation of intestinal stem cells. The distinct expression of glycans may reflect the functional roles of niche cells. BC2LCN is a valuable tool for investigating the functional significance of protein glycosylation in stem cell regulation.
Department of Anatomy, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo 181-8611, Japan.
Daisuke
D
Sugahara
J-Oil Mills, Inc., 11 Kagetoricho, Totsuka-ku, Yokohama, Kanagawa 245-0064, Japan.
Yuka
Y
Kobayashi
Department of Anatomy, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo 181-8611, Japan.
Yoshihiro
Y
Akimoto
Department of Anatomy, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo 181-8611, Japan.
Hayato
H
Kawakami
D000818:Animals; D057508:Burkholderia cenocepacia / Q000737:chemistry; D019070:Cell Lineage / Q000235:genetics*; D003106:Colon / Q000737:chemistry / Q000166:cytology; D020397:Goblet Cells / Q000737:chemistry / Q000378:metabolism; D007082:Ileum / Q000737:chemistry / Q000166:cytology; D037102:Lectins / Q000737:chemistry*; D051379:Mice; D000066450:Mouse Embryonic Stem Cells / Q000737:chemistry / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D019009:Proto-Oncogene Proteins c-kit / Q000302:isolation & purification / Q000378:metabolism*; D055153:Stem Cell Niche / Q000235:genetics
D037102:Lectins; D011134:Polysaccharides; D019009:Proto-Oncogene Proteins c-kit