Genome-wide indel/SSR scanning reveals significant loci associated with excellent agronomic traits of a cabbage (Brassica oleracea) elite parental line '01-20'.
Elite parental lines are of great significance to crop breeding. To discover unique genomic loci associated with excellent economic traits in the elite cabbage inbred-line '01-20', we performed comparisons of phenotypes as well as whole-genome insertion-deletion/simple sequence repeat loci between '01-20' and each of its five sister lines. '01-20' has a range of excellent agronomic traits, including early-maturing, and improvements in plant type and leaf colour. Eight unique loci were discovered for '01-20' and '01-07-258', another elite line similar to '01-20' at the whole-genome level. In addition, two excellent double-haploid lines derived from a cross of '01-20' also inherited these loci. Based on the quantitative trait locus association results, five of these loci were found to be associated with important agronomic traits, which could explain why the elite parent '01-20' possesses greener outer leaves, a more compact and upright plant-type, rounder head, shorter core length, and better taste. Additionally, some of these loci have clustering effects for quantitative trait loci associated with different traits; therefore, important genes in these regions were analysed. The obtained results should enable marker-assisted multi-trait selection at the whole-genome level in cabbage breeding and provide insights into significant genome loci and their breeding effects.
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, 12# Zhongguancun Nandajie, Beijing 100081, China.
Honghao
H
Lv
Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, 50# Zhanghua Street, Beijing 100097, China.
Qingbiao
Q
Wang
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, 12# Zhongguancun Nandajie, Beijing 100081, China.
Fengqing
F
Han
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, 12# Zhongguancun Nandajie, Beijing 100081, China.
Xing
X
Liu
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, 12# Zhongguancun Nandajie, Beijing 100081, China.
Zhiyuan
Z
Fang
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, 12# Zhongguancun Nandajie, Beijing 100081, China.
Limei
L
Yang
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, 12# Zhongguancun Nandajie, Beijing 100081, China.
Mu
M
Zhuang
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, 12# Zhongguancun Nandajie, Beijing 100081, China.
Yumei
Y
Liu
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, 12# Zhongguancun Nandajie, Beijing 100081, China.
Zhansheng
Z
Li
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, 12# Zhongguancun Nandajie, Beijing 100081, China.
Yangyong
Y
Zhang
D001937:Brassica / Q000235:genetics*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D020869:Gene Expression Profiling; D055106:Genome-Wide Association Study; D005838:Genotype; D054643:INDEL Mutation; D018895:Microsatellite Repeats; D010641:Phenotype; D040641:Quantitative Trait Loci; D019655:Quantitative Trait, Heritable