Isolation, one-step affinity purification, and characterization of a polyextremotolerant laccase from the halophilic bacterium Aquisalibacillus elongatus and its application in the delignification of sugar beet pulp.
The aim of the present work was to study the ability of a halophilic bacterial laccase to efficient delignification in extreme conditions. Here, a highly stable extracellular laccase showing ligninolytic activity from halophilic Aquisalibacillus elongatus is described. The laccase production was strongly influenced by NaCl and CuSO4and under optimal conditions reached 4.8UmL-1. The monomeric enzyme of 75kDa was purified by a synthetic affinity column with 68.2% yield and 99.8-fold purification. The enzyme showed some valuable features viz. stability against a wide range of organic solvents, salts, metals, inhibitors, and surfactants and specificity to a wide spectrum of substrates diverse in structure and redox potential. It retained more than 50% of the original activity at 25-75°C and pH 5.0-10.0. Furthermore, the enzyme was found to be effective in the delignification of sugar beet pulp in an ionic liquid that makes it useful for industrial applications.
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran 1417614411, Iran.
Shahla
S
Rezaei
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran 1417614411, Iran.
Ahmad Reza
AR
Shahverdi
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran 1417614411, Iran. Electronic address: faramarz@tums.ac.ir.
Mohammad Ali
MA
Faramarzi
D001407:Bacillus / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D027461:Beta vulgaris / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D004795:Enzyme Stability / Q000187:drug effects; D007526:Isoelectric Point; D007700:Kinetics; D042845:Laccase / Q000302:isolation & purification* / Q000378:metabolism; D008031:Lignin / Q000302:isolation & purification*; D008970:Molecular Weight; D009930:Organic Chemicals / Q000494:pharmacology; D010802:Phylogeny; D012492:Salts / Q000494:pharmacology; D012997:Solvents / Q000494:pharmacology; D013696:Temperature
D009930:Organic Chemicals; D012492:Salts; D012997:Solvents; D008031:Lignin; D042845:Laccase
Affinity chromatography; Aquisalibacillus elongatus; Delignification; Halophilic laccase; Ionic liquid