Dietary Selenomethionine Administration in the American Alligator (Alligator mississippiensis): Hepatic and Renal Se Accumulation and Its Effects on Growth and Body Condition.
Selenium (Se) is an essential trace nutrient, but in excess, it can induce toxicity. Incomplete combustion of coal produces coal combustion wastes, which are enriched in Se and often disposed of in aquatic basins. While a multitude of studies have investigated Se accumulation in vertebrates, few studies have examined its effects on longer-lived top trophic carnivores, such as the American alligator (Alligator mississippiensis). In this study, alligators were fed one of three Dietary Treatments: mice injected with water (controls) or water supplemented with 1000 or 2000 ppm selenomethionine (SeMet). Dietary Treatment significantly affected Se levels in both the liver (p < 0.0001; raw mean ± SE: 1000 ppm group, 35.20 ± 6.32 ppm; 2000 ppm group, 49.97 ± 4.00 ppm) and kidney (p < 0.0001; raw mean ± SE: 1000 ppm group, 101.60 ± 8.64 ppm; 2000 ppm, 96.38 ± 5.81 ppm), which were significantly higher in alligators fed SeMet than in controls. Post-treatment head length, used to control for size variation, was negatively related to both kidney (p = 0.0142) and liver (p = 0.0010) Se concentrations. Dietary treatment with SeMet significantly reduced body condition (1000 ppm, p < 0.0029; 2000 ppm, p = 0.0075), but it significantly increased growth (1000 ppm, p < 0.0001; 2000 ppm, p = 0.0316). Body condition and growth remained unchanged in control alligators (p > 0.05). Our results demonstrate alligators are capable of accumulating high levels of Se through trophic transfer. The positive effects of accumulation on growth may demonstrate Se essentiality, whereas the negative effects on condition may demonstrate toxicity. Accumulation also was associated with mortality, further demonstrating toxicity. Future studies should further investigate the physiological effects of Se accumulation in long-lived, top-trophic carnivores.
Department of Environmental Health Science, University of Georgia, Athens, GA, 30602, USA. jwf0016@auburn.edu.|Savannah River Ecology Laboratory, University of Georgia, PO Drawer E, Aiken, SC, 29802, USA. jwf0016@auburn.edu.|Interdisciplinary Toxicology Program, University of Georgia, Athens, GA, 30602, USA. jwf0016@auburn.edu.|Department of Biological Sciences, Auburn University, Auburn, AL, 36849, USA. jwf0016@auburn.edu.
John W
JW
Finger
Savannah River Ecology Laboratory, University of Georgia, PO Drawer E, Aiken, SC, 29802, USA.
Matthew T
MT
Hamilton
Department of Environmental Health Science, University of Georgia, Athens, GA, 30602, USA.|Interdisciplinary Toxicology Program, University of Georgia, Athens, GA, 30602, USA.
Travis C
TC
Glenn
Savannah River Ecology Laboratory, University of Georgia, PO Drawer E, Aiken, SC, 29802, USA.|Interdisciplinary Toxicology Program, University of Georgia, Athens, GA, 30602, USA.
Tracey D
TD
Tuberville
D000489:Alligators and Crocodiles / Q000502:physiology*; D000818:Animals; D004032:Diet; D007668:Kidney / Q000502:physiology*; D008099:Liver / Q000502:physiology*; D012643:Selenium / Q000378:metabolism*; D012645:Selenomethionine / Q000378:metabolism / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
D014874:Water Pollutants, Chemical; D012645:Selenomethionine; D012643:Selenium