Immune Signature of Enhanced Functional Avidity CD8+T Cells in vivo Induced by Vaccinia Vectored Vaccine.
Functional avidity of T cells is a critical determinant for clearing viral infection and eliminating tumor. Understanding how functional avidity is maintained in T cells is imperative for immunotherapy. However, studies systematically characterize T cell with high functional avidity induced in vivo are still lacking. Previously, we and others found vaccinia vectored vaccine (VACV) induced antigen-specific CD8+T cells with relatively high functional avidity to those from DNA vaccine. Herein, we used functional, immune phenotyping and transcriptomic studies to define the immune signature of these CD8+T cells with high functional avidity. Antigen-specific CD8+T cells induced by VACV executed superior in vivo killing activity and displayed a distinct transcriptional profile, whereas no significantly differences were found in composition of memory sub-populations and cytokine poly-functionality. Transcriptional analyses revealed unique features of VACV induced CD8+T cells in several biological processes, including transport, cell cycle, cell communication and metabolic processes. In summary, we characterize CD8+T cells of high functional avidity induced in vivo by VACV, which not only improves our understanding of adaptive T cell immunity in VACV vaccination, but also provides clues to modulate functional avidity of CD8+T cells for T cell based immunotherapy.
Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Zhidong
Z
Hu
Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Lingyan
L
Zhu
Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Jing
J
Wang
Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Yanmin
Y
Wan
Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Songhua
S
Yuan
Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Jian
J
Chen
Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Xiangqing
X
Ding
Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Chenli
C
Qiu
Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.|Institutes of Biomedical Sciences, Fudan University, Shanghai, China.|Key Laboratory of Medical Molecular Virology of MOE/ MOH, Fudan University, Shanghai, China.
Xiaoyan
X
Zhang
Institutes of Biomedical Sciences, Fudan University, Shanghai, China.|Key Laboratory of Medical Molecular Virology of MOE/ MOH, Fudan University, Shanghai, China.
Chao
C
Qiu
Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.|Institutes of Biomedical Sciences, Fudan University, Shanghai, China.|Key Laboratory of Medical Molecular Virology of MOE/ MOH, Fudan University, Shanghai, China.
Jianqing
J
Xu
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D016207:Cytokines; D003602:Cytotoxicity, Immunologic; D004195:Disease Models, Animal; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D020869:Gene Expression Profiling; D005822:Genetic Vectors / Q000235:genetics*; D007114:Immunization; D007156:Immunologic Memory; D051379:Mice; D008954:Models, Biological; D053594:Myeloid Differentiation Factor 88 / Q000378:metabolism; D015398:Signal Transduction; D037182:T-Cell Antigen Receptor Specificity; D059467:Transcriptome; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics*; D014765:Viral Vaccines / Q000235:genetics* / Q000276:immunology*
D016207:Cytokines; D018984:Epitopes, T-Lymphocyte; D053594:Myeloid Differentiation Factor 88; D019444:Vaccines, DNA; D014765:Viral Vaccines