Generation of in vivo neural stem cells using partially reprogrammed cells defective in in vitro differentiation potential.
Pluripotent stem cells can be easily differentiated in vitro into a certain lineage through embryoid body formation. Recently, however, we reported partially reprogrammed cells showing some pluripotent characteristics, which failed to differentiate in vitro. Here, we attempted to generate neural stem cells (NSCs) from partially reprogrammed cells using an in vivo differentiation system involving teratoma formation. Partially reprogrammed cells formed teratomas after injection into immunocompromised mice, and NSCs could be isolated from these teratomas. These in vivo NSCs expressed NSC markers and terminally differentiated into neurons and glial cells. Moreover, these NSCs exhibited molecular profiles very similar to those of brain-derived NSCs. These results suggest that partially reprogrammed cells defective in in vitro differentiation ability can differentiate into pure populations of NSCs through an in vivo system.
Department of Stem Cell and Regenerative Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Seoul, Republic of Korea.
Jong Soo
JS
Kim
Department of Stem Cell and Regenerative Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Seoul, Republic of Korea.
Yean Ju
YJ
Hong
Department of Stem Cell and Regenerative Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Seoul, Republic of Korea.
Hyun Woo
HW
Choi
Department of Stem Cell and Regenerative Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Seoul, Republic of Korea.
Hyuk
H
Song
Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Suwon, Republic of Korea.
Sung June
SJ
Byun
Department of Stem Cell and Regenerative Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Seoul, Republic of Korea.
Jeong Tae
JT
Do
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D065150:Cellular Reprogramming / Q000502:physiology*; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008822:Mice, Transgenic; D058953:Neural Stem Cells / Q000166:cytology*; D039904:Pluripotent Stem Cells / Q000166:cytology; D013724:Teratoma / Q000473:pathology
differentiation; iPSCs; neural stem cells; pluripotent stem cells; teratoma