Mimicking Horseradish Peroxidase and NADH Peroxidase by Heterogeneous Cu2+-Modified Graphene Oxide Nanoparticles.
Cu2+-ion-modified graphene oxide nanoparticles, Cu2+-GO NPs, act as a heterogeneous catalyst mimicking functions of horseradish peroxidase, HRP, and of NADH peroxidase. The Cu2+-GO NPs catalyze the oxidation of dopamine to aminochrome by H2O2and catalyze the generation of chemiluminescence in the presence of luminol and H2O2. The Cu2+-GO NPs provide an active material for the chemiluminescence detection of H2O2and allow the probing of the activity of H2O2-generating oxidases and the detection of their substrates. This is exemplified with detecting glucose by the aerobic oxidation of glucose by glucose oxidase and the Cu2+-GO NP-stimulated chemiluminescence intensity generated by the H2O2product. Similarly, the Cu2+-GO NPs catalyze the H2O2oxidation of NADH to the biologically active NAD+cofactor. This catalytic system allows its conjugation to biocatalytic transformations involving NAD+-dependent enzyme, as exemplified for the alcohol dehydrogenase-catalyzed oxidation of benzyl alcohol to benzoic acid through the Cu2+-GO NPs-catalyzed regeneration of NAD+.
Institute of Chemistry, The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
Shan
S
Wang
Institute of Chemistry, The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
Rémi
R
Cazelles
Institute of Chemistry, The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
Wei-Ching
WC
Liao
Institute of Chemistry, The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
Margarita
M
Vázquez-González
Institute of Chemistry, The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
Amani
A
Zoabi
Institute of Chemistry, The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
Raed
0000-0003-2342-9902
R
Abu-Reziq
Institute of Chemistry, The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
Itamar
0000-0001-9710-9077
I
Willner
D040761:Biomimetic Materials / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods; D002384:Catalysis; D002413:Cations, Divalent / Q000737:chemistry; D003300:Copper / Q000737:chemistry*; D005947:Glucose / Q000032:analysis; D006108:Graphite / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods; D008165:Luminol / Q000032:analysis; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D010544:Peroxidases / Q000737:chemistry*
D002413:Cations, Divalent; D010087:Oxides; D008165:Luminol; D006108:Graphite; D003300:Copper; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D010544:Peroxidases; C021845:NAD+ peroxidase; D005947:Glucose
Catalysis; biocatalysis; chemiluminescence; dopamine; nanotechnology