Multiclonal Expansion of Klebsiella pneumoniae Isolates Producing NDM-1 in Rio de Janeiro, Brazil.
We characterized NDM-1-producingKlebsiellaisolates from Rio de Janeiro, Brazil. PCR was applied for resistance and virulence determinants. The genetic context ofblaNDMwas determined by S1 nuclease pulsed-field gel electrophoresis (PFGE) and hybridization. Genotyping was performed by PFGE and multilocus sequence typing (MLST). Most isolates carried multiple resistance genes and remained susceptible to amikacin, fosfomycin-trometamol, polymyxin B, and tigecycline. The spread of NDM-1-producingKlebsiella pneumoniaewas not associated with clonal expansion and appears to be associated with Tn3000.
Laboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil.
Caio Augusto Martins
CA
Aires
Laboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil.
Polyana Silva
PS
Pereira
Laboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil.
Carlos Felipe Machado
CF
de Araujo
Laboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil.
Thiago Pavoni Gomes
TP
Chagas
Laboratório Central de Saúde Pública Noel Nutels, Rio de Janeiro, Brazil.
Jane Cleide Ribeiro
JC
Oliveira
Coordenação Estadual de Controle de Infecção Hospitalar, Secretaria Estadual de Saúde, Rio de Janeiro, Brazil.
Sibelle Nogueira
SN
Buonora
Departamento de Bioquímica, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil.
Rodolpho Mattos
RM
Albano
Laboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil.
Ana Paula D'Alincourt
AP
Carvalho-Assef
Laboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil marise@ioc.fiocruz.br.
Marise Dutra
MD
Asensi
D000583:Amikacin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015373:Bacterial Typing Techniques; D001938:Brazil / Q000453:epidemiology; D002999:Clone Cells; D004251:DNA Transposable Elements; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D005578:Fosfomycin / Q000494:pharmacology; D015870:Gene Expression; D005838:Genotype; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology / Q000382:microbiology / Q000635:transmission; D007711:Klebsiella pneumoniae / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D008911:Minocycline / Q000031:analogs & derivatives / Q000494:pharmacology; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D011112:Polymyxin B / Q000494:pharmacology; D000078304:Tigecycline; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D005578:Fosfomycin; D000078304:Tigecycline; D000583:Amikacin; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1; D008911:Minocycline; D011112:Polymyxin B
Klebsiella pneumoniae; MLST; NDM carbapenemase; New Delhi metallo-β-lactamase; molecular typing