Oxalic Acid fromLentinula edodesCulture Filtrate: Antimicrobial Activity on Phytopathogenic Bacteria and Qualitative and Quantitative Analyses.
The culture filtrate ofLentinula edodesshows potent antimicrobial activity against the plant pathogenic bacteriaRalstonia solanacearum. Bioassay-guided fractionation was conducted using Diaion HP-20 column chromatography, and the insoluble active compound was not adsorbed on the resin. Further fractionation by high-performance liquid chromatography (HPLC) suggested that the active compounds were organic acids. Nine organic acids were detected in the culture filtrate ofL. edodes; oxalic acid was the major component and exhibited antibacterial activity against nine different phytopathogenic bacteria. Quantitative analysis by HPLC revealed that the content of oxalic acid was higher in the water extract from spent mushroom substrate than in liquid culture. This suggests that the water extract of spentL. edodessubstrate is an eco-friendly control agent for plant diseases.
Graduate School of Future Convergence Technology, Hankyong National University, Anseong 17579, Korea.
A-Min
AM
Kwak
Division of Biotechnology and Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 54596, Korea.
In-Kyoung
IK
Lee
Agricultural Microbiology Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA), Wanju 54875, Korea.
Sang-Yeop
SY
Lee
Division of Biotechnology and Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 54596, Korea.
Bong-Sik
BS
Yun
Graduate School of Future Convergence Technology, Hankyong National University, Anseong 17579, Korea.; Institute of Genetic Engineering, Hankyong National University, Anseong 17579, Korea.; Department of Horticultural Life Science, Hankyong National University, Anseong 17579, Korea.
Hee-Wan
HW
Kang
Antibacterial activity; Oxalic acid; Qualitative and quantitative analyses