Lack of association between polymorphism rs540782 and primary open angle glaucoma in Saudi patients.
BACKGROUND
To investigate whether polymorphism rs540782 on chromsome 1, in close proximity to the Zona Pellucida Glycoprotein 4 (ZP4) gene, is a risk factor for primary open angle glaucoma (POAG).


METHODS
The study genotyped 92 unrelated POAG cases and 95 control subjects from Saudi Arabia using Taq-Man® assay.


RESULTS
The genotype frequency distribution did not deviate significantly from the Hardy-Weinberg equilibrium (p > 0.05). Overall, both the genotype and allele frequencies were not significantly different between cases and controls. The minor 'C' allele frequency was 49.4%, which was comparable to the Japanese population and higher than the Indian and Afro-Caribbean populations. Similarly, no significant association was found between genotypes and systemic diseases and health awareness/behavior domain variables. Importantly, glaucoma specific indices, such as intraocular pressure, cup/disc ratio and number of anti-glaucoma medication, also showed no statistically significant effect of genotypes within POAG cases.


CONCLUSIONS
Polymorphism rs540782 is not a risk factor for POAG in the Saudi cohort.
Glaucoma Research Chair, Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, 11411, Saudi Arabia.
Altaf A
AA
Kondkar
Case Western Reserve University, Ohio, USA.
Nikhil B
NB
Edward
Glaucoma Research Chair, Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, 11411, Saudi Arabia.
Hatem
H
Kalantan
Glaucoma Research Chair, Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, 11411, Saudi Arabia.
Abdullah S
AS
Al-Kharashi
SAAD Research & Development Center, Clinical Research Lab., SAAD Specialist Hospital, P.O. Box 30353, Al Khobar, 31952, Saudi Arabia.|Qassim University, Buraydah, Saudi Arabia.
Saleh
S
Altuwaijri
Disease Control Strategy Group, Liverpool School of Tropical Medicine, Liverpool, UK.
Gamal
G
Mohamed
Glaucoma Research Chair, Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, 11411, Saudi Arabia.
Tahira
T
Sultan
Glaucoma Research Chair, Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, 11411, Saudi Arabia.
Taif A
TA
Azad
Glaucoma Research Chair, Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, 11411, Saudi Arabia. abuamero@gmail.com.|Department of Ophthalmology, College of Medicine, University of Florida, Jacksonville, FL, USA. abuamero@gmail.com.|Ophthalmic Genetics Laboratory, Department of Ophthalmology, College of Medicine, King Saud University, P.O. Box 245, Riyadh, 11411, Saudi Arabia. abuamero@gmail.com.
Khaled K
KK
Abu-Amero
D016022:Case-Control Studies; D003710:Demography; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005902:Glaucoma, Open-Angle / Q000235:genetics*; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012529:Saudi Arabia
Middle-east; POAG; ZP4; rs540782