High-Throughput Toxicity and Phenotypic Screening of 3D Human Neural Progenitor Cell Cultures on a Microarray Chip Platform.
A 3D cell culture chip was used for high-throughput screening of a human neural progenitor cell line. The differential toxicity of 24 compounds was determined on undifferentiated and differentiating NPCs. Five compounds led to significant differences in IC50values between undifferentiated and differentiating cultures. This platform has potential use in phenotypic screening to elucidate molecular toxicology on human stem cells.
Chemical and Biological Engineering and Center for Biotechnology & Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
Gregory J
GJ
Nierode
Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA.
Brian C
BC
Perea
Bioengineering, University of California, Berkeley, CA 94720, USA.
Sean K
SK
McFarland
Department of Bioengineering and Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Lisbon 1049-001, Portugal.
Jorge F
JF
Pascoal
Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA.
Douglas S
DS
Clark
Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA; Bioengineering, University of California, Berkeley, CA 94720, USA.
David V
DV
Schaffer
Chemical and Biological Engineering and Center for Biotechnology & Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA. Electronic address: dordick@rpi.edu.
Jonathan S
JS
Dordick
D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D055808:Drug Discovery; D057166:High-Throughput Screening Assays; D006801:Humans; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D023382:Toxicity Tests, Acute
high-content screening; high-throughput microarray; human neural stem cells; neurotoxicity; screening; three-dimensional cell culture