In situ fabrication of label-free optical sensing paper strips for the rapid surface-enhanced Raman scattering (SERS) detection of brassinosteroids in plant tissues.
A surface-enhanced Raman scattering (SERS)-functionalized, gold nanoparticle (AuNPs)-immobilized paper strip was developed to realize label-free rapid detection of brassinosteroids (BRs) in plant tissues. The AuNPs paper strip was fabricated through utilizing poly(γ-glutamic acid) (γ-PGA) as the linker and concentrator for the directly immobilized AuNPs on paper, which can circumvent many drawbacks of the existing SERS substrates. An enhancement factor of 1.13×108, high reproducibility (relative standard deviation of 4.78%), highly sensitive detection limit with a correlation coefficient of 0.99 (1×10-11M 4-mercaptophenylboronic acid), and long-term stability for the responses of the SERS-functionalized AuNPs paper strip to Raman intensities were achieved by optimizing reaction conditions including the concentrations of γ-PGA and HAuCl4/NaBH4. Ultimately, this developed paper strip was applied to the determination of BRs in plant tissues, obtaining precisions with RSD values in a range of 3.0-13.8%. The SERS-functionalized AuNPs paper strip featuring label-free rapid analysis, and high sensitivity could serve as an excellent alternative for on-site rapid detections.
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Miao
M
Chen
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Zhimin
Z
Zhang
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Minzhuo
M
Liu
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Chen
C
Qiu
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China. Electronic address: hyangchem@csu.edu.cn.
Hua
H
Yang
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China. Electronic address: xqchen@csu.edu.cn.
Xiaoqing
X
Chen
D015374:Biosensing Techniques / Q000379:methods*; D060406:Brassinosteroids / Q000032:analysis*; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D061848:Optical Imaging / Q000379:methods*; D010209:Paper; D010944:Plants / Q000378:metabolism*; D011934:Reagent Strips / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013499:Surface Properties
D060406:Brassinosteroids; D011934:Reagent Strips; D006046:Gold
Brassinosteroids; Paper strip; Surface-enhanced Raman scattering (SERS)