A potential bioactive wound dressing based on carboxymethyl cellulose/ZnO impregnated MCM-41 nanocomposite hydrogel.
Lack of antibacterial activity, deficient water vapor and oxygen permeability, and insufficient mechanical properties are disadvantages of existing wound dressings. Hydrogels could absorb wound exudates due to their strong swelling ratio and give a cooling sensation and a wet environment. To overcome these shortcomings, flexible nanocomposite hydrogel films was prepared through combination of zinc oxide impregnated mesoporous silica (ZnO-MCM-41) as a nano drug carrier with carboxymethyl cellulose (CMC) hydrogel. Citric acid was used as cross linker to avoid the cytotoxicity of conventional cross linkers. The prepared nanocomposite hydrogel was characterized using X-ray diffractometry (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Zeta potential and UV-vis spectroscopy. Results of swelling and erosion tests showed CMC/ZnO nanocomposite hydrogel disintegrated during the first hours of the test. Using MCM-41 as a substrate for ZnO nanoparticles solved this problem and the CMC/ZnO-MCM-41 showed a great improvement in tensile strength (12%), swelling (100%), erosion (53%) and gas permeability (500%) properties. Drug delivery and antibacterial properties of the nanocomposite hydrogel films studied using tetracycline (TC) as a broad spectrum antibiotic and showed a sustained TC release. This could efficiently decrease bandage exchange. Cytocompatibility of the nanocomposite hydrogel films has been analyzed in adipose tissue-derived stem cells (ADSCs) and results showed cytocompatibility of CMC/ZnO-MCM-41. Based on these results the prepared CMC nanocomposite hydrogel containing ZnO impregnated MCM-41, could serve as a kind of promising wound dressing with sustained drug delivery properties.
Research Laboratory of Dendrimers and Nanopolymers, Faculty of Chemistry, University of Tabriz, P.O. Box 51666, Tabriz, Iran.
Rasul
R
Rakhshaei
Research Laboratory of Dendrimers and Nanopolymers, Faculty of Chemistry, University of Tabriz, P.O. Box 51666, Tabriz, Iran; Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Science, Tabriz, Iran. Electronic address: namazi@tabrizu.ac.ir.
Hassan
H
Namazi
D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001458:Bandages; D001672:Biocompatible Materials / Q000494:pharmacology*; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D065546:Drug Liberation; D004926:Escherichia coli / Q000187:drug effects; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010539:Permeability; D012822:Silicon Dioxide / Q000737:chemistry*; D055672:Static Electricity; D013227:Steam; D013234:Stem Cells / Q000166:cytology; D013718:Tensile Strength; D013752:Tetracycline / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects*; D014961:X-Ray Diffraction; D015034:Zinc Oxide / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; C509968:MCM-41; D013227:Steam; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D012822:Silicon Dioxide; D013752:Tetracycline; D002266:Carboxymethylcellulose Sodium; D015034:Zinc Oxide
Drug delivery; Hydrogel nanocomposite; Mesoporous silica; Wound dressing; Zinc oxide