Diminished Antimicrobial Peptide and Antifungal Antibiotic Activities against Candida albicans in Denture Adhesive.
The underlying causes of denture stomatitis may be related to the long-term use of adhesives, which may predispose individuals to oral candidiasis. In this study, we hypothesize that antimicrobial peptides and antifungal antibiotics have diminished anti-Candidaactivities in denture adhesive. To show this, nine antimicrobial peptides and five antifungal antibiotics with and without 1.0% denture adhesive were incubated withCandida albicansstrains ATCC 64124 and HMV4C in radial diffusion assays. In gels with 1.0% adhesive, HNP-1, HBD2, HBD3, IP-10, LL37 (only one strain), histatin 5 (only one strain), lactoferricin B, and SMAP28 showed diminished activity againstC. albicans. In gels with 1.0% adhesive, amphotericin B and chlorhexidine dihydrochloride were active against both strains ofC. albicans. These results suggest that denture adhesive may inactivate innate immune mediators in the oral cavity increasing the risk ofC. albicansinfections, but inclusion of antifungal antibiotics to denture adhesive may aid in prevention or treatment ofCandidainfections and denture stomatitis.
Iowa Institute for Oral Health Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA. amber-bates@uiowa.edu.
Amber M
AM
Bates
Department of Restorative Dentistry, School of Dentistry, Oregon Health and Science University, Portland, OR 97201, USA. garaicoa@ohsu.edu.
Jorge L
JL
Garaicoa
Department of Biology, Waldorf University, Forest City, IA 50436, USA. Carol.Fischer@waldorf.edu.
Carol L
CL
Fischer
Iowa Institute for Oral Health Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA. kim-brogden@uiowa.edu.
Kim A
KA
Brogden
Candida albicans; antifungal antibiotics; antimicrobial peptides; denture adhesive; radial diffusion assay