Sensitive fluorescence detection of ATP based on host-guest recognition between near-infrared β-Cyclodextrin-CuInS2QDs and aptamer.
We have developed a near-infrared (NIR) fluorescent aptamer-based sensor for sensitive detection of adenosine-5'-triphosphate (ATP) by using a ATP-binding aptamer and β-Cyclodextrin-CuInS2quantum dots (β-CD-CuInS2QDs). The fluorescence of β-CD-CuInS2QDs has a slight enhancement with the addition of ATP-binding aptamer due to the host-guest recognition between aptamer and β-CD. When ATP is added, it will bind to aptamer to form G-quadruplexes. Aptamer-ATP complexes can enter into the hydrophobic cavities of β-CD and result in great enhancement of the fluorescence intensity. Under the optimum conditions, the fluorescence intensity of β-CD-CuInS2QDs is proportional to the concentration of ATP, which shows a good linear response toward ATP concentration range of 6-1200μmolL-1, the detection limit for ATP is 3μmolL-1. The present assay shows a good selectivity for ATP over other biologically important proteins, and it is applied to the determination of ATP in human serum sample with satisfactory results.
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
Tianyu
T
Hu
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
Weidan
W
Na
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
Xu
X
Yan
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China. Electronic address: suxg@jlu.edu.cn.
Xingguang
X
Su
D000255:Adenosine Triphosphate / Q000097:blood*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D045663:Quantum Dots; D047392:beta-Cyclodextrins / Q000737:chemistry*
D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D047392:beta-Cyclodextrins; D003300:Copper; D000255:Adenosine Triphosphate
Aptamer; CuInS(2) QDs; Fluorescence; Host-guest recognition; Near-infrared; β-Cyclodextrin