Antioxidant and Cytoprotective Activities of Fucus spiralis Seaweed on a Human Cell in Vitro Model.
Antioxidants play an important role as Reactive Oxygen Species (ROS) chelating agents and, therefore, the screening for potent antioxidants from natural sources as potential protective agents is of great relevance. The main aim of this study was to obtain antioxidant-enriched fractions from the common seaweedFucus spiralisand evaluate their activity and efficiency in protecting human cells (MCF-7 cells) on an oxidative stress condition induced by H₂O₂. Five fractions, F1-F5, were obtained by reversed-phase vacuum liquid chromatography. F3, F4 and F5 revealed the highest phlorotannin content, also showing the strongest antioxidant effects. The cell death induced by H₂O₂ was reduced by all fractions following the potency order F4 > F2 > F3 > F5 > F1. Only fraction F4 completely inhibited the H₂O₂ effect. To understand the possible mechanisms of action of these fractions, the cellular production of H₂O₂, the mitochondrial membrane potential and the caspase 9 activity were studied. Fractions F3 and F4 presented the highest reduction on H₂O₂ cell production. All fractions decreased both caspase-9 activity and cell membrane depolarization (except F1). Taken all together, the edibleF. spiralisreveal that they provide protection against oxidative stress induced by H₂O₂ on the human MCF-7 cellular model, probably acting as upstream blockers of apoptosis.
MARE-Marine and Environmental Sciences Centre, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal. susete.pinteus@ipleiria.pt.
Susete
S
Pinteus
MARE-Marine and Environmental Sciences Centre, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal. joana.m.silva@ipleiria.pt.
Joana
J
Silva
MARE-Marine and Environmental Sciences Centre, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal. celso.alves@ipleiria.pt.
Celso
C
Alves
MARE-Marine and Environmental Sciences Centre, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal. andre.horta@ipleiria.pt.
André
A
Horta
Marine Biodiscovery, School of Chemistry, National University of Ireland Galway, University Road, H91TK33 Galway, Ireland. olivier.thomas@nuigalway.ie.
Olivier P
OP
Thomas
MARE-Marine and Environmental Sciences Centre, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal. rpedrosa@ipleiria.pt.
Rui
R
Pedrosa
D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001688:Biological Products / Q000737:chemistry / Q000494:pharmacology; D053453:Caspase 9 / Q000378:metabolism; D005591:Chemical Fractionation; D019610:Cytoprotection; D044645:Fucus / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D061986:MCF-7 Cells; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012624:Seaweed / Q000737:chemistry*
D000975:Antioxidants; D001688:Biological Products; D010636:Phenols; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D053453:Caspase 9
MCF-7 cells; edible algae; marine natural products; oxidative stress; phlorotannins; reactive oxygen species