Aza-heterocyclic Receptors for Direct Electron Transfer Hemoglobin Biosensor.
Direct Electron Transfer biosensors, facilitating direct communication between the biomolecule of interest and electrode surface, are preferable compared to enzymatic and mediator based sensors. Although hemoglobin (Hb) contains four redox active iron centres, direct detection is not possible due to inaccessibility of iron centres and formation of dimers, blocking electron transfer. Through the coordination of iron with aza-heterocyclic receptors - pyridine and imidazole - we report a cost effective, highly sensitive and simple electrochemical Hb sensor using cyclic voltammetry and chronoamperometry. The receptor can be either in the form of liquid micro-droplet mixed with blood or dry chemistry embedded in paper membrane on top of screen printed carbon electrodes. We demonstrate excellent linearity and robustness against interference using clinical samples. A truly point of care technology is demonstrated by integrating disposable test strips with handheld reader, enabling finger prick to result in less than a minute.
Centre for Nano Science and Engineering, Indian Institute of Science, Bangalore-560012, India.|PathShodh Healthcare Pvt Ltd, Bangalore-560012, India.
Vinay
V
Kumar
PathShodh Healthcare Pvt Ltd, Bangalore-560012, India.
D M Nikhila
DM
Kashyap
PathShodh Healthcare Pvt Ltd, Bangalore-560012, India.
Suraj
S
Hebbar
Anand Diagnostics Laboratory, Bangalore, India.
R
R
Swetha
Anand Diagnostics Laboratory, Bangalore, India.
Sujay
S
Prasad
Samatvam Diabetes Endocrinology Centre, Bangalore, India.
T
T
Kamala
Samatvam Diabetes Endocrinology Centre, Bangalore, India.
S S
SS
Srikanta
Centre for Nano Science and Engineering, Indian Institute of Science, Bangalore-560012, India.
P R
PR
Krishnaswamy
Centre for Nano Science and Engineering, Indian Institute of Science, Bangalore-560012, India.
Navakanta
N
Bhat
D001372:Aza Compounds / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D055664:Electrochemical Techniques; D004566:Electrodes; D004579:Electron Transport; D004583:Electrons; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D007093:Imidazoles / Q000737:chemistry; D007501:Iron / Q000737:chemistry; D011725:Pyridines / Q000737:chemistry; D011934:Reagent Strips; D062165:Receptors, Artificial / Q000737:chemistry*; D012680:Sensitivity and Specificity
D001372:Aza Compounds; D006454:Hemoglobins; D007093:Imidazoles; D011725:Pyridines; D011934:Reagent Strips; D062165:Receptors, Artificial; D002244:Carbon; C029899:imidazole; D007501:Iron; C023666:pyridine