Conjugation of poly(ethylene glycol) to poly(lactide)-based polyelectrolytes: An effective method to modulate cytotoxicity in gene delivery.
Positively charged amphiphilic polymers comprising of poly(N,N-dimethylaminoethyl methacrylate) segments on both sides of a poly(l-lactic acid) segment was conjugated with poly(ethylene glycol) (DMA-PLLA-DMA@PEG). Efficient condensation of plasmid DNA by these polymers was shown. Cell toxicity studies demonstrated that the positively charged polymers with attached PEG exhibited much less cytotoxicity than polymers with no PEG in both HEK293 and Hela cell lines. PEGylation also resulted in polymers with enhanced haemo-compatibility. The positively charged polymers displayed very good DNA plasmid delivery efficiencies in both HEK293 and Hela cells.
School of Chemistry and Chemical Engineering, Henan Normal University, China.
Xiaoshan
X
Fan
Institute of Materials Research and Engineering (IMRE), A*STAR, 2 Fusionopolis Way, Innovis, #08-03, 138634, Singapore.
Shan
S
Jiang
Institute of Materials Research and Engineering (IMRE), A*STAR, 2 Fusionopolis Way, Innovis, #08-03, 138634, Singapore. Electronic address: lizb@imre.a-star.edu.sg.
Zibiao
Z
Li
Institute of Materials Research and Engineering (IMRE), A*STAR, 2 Fusionopolis Way, Innovis, #08-03, 138634, Singapore; Department of Materials Science and Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117576, Singapore; Singapore Eye Research Institute, 11 Third Hospital Avenue, Singapore 168751, Singapore. Electronic address: lohxj@imre.a-star.edu.sg.
Xian Jun
XJ
Loh
D016923:Cell Death; D002470:Cell Survival; D004247:DNA / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D018014:Gene Transfer Techniques; D057809:HEK293 Cells; D006367:HeLa Cells; D006461:Hemolysis; D006801:Humans; D008689:Methacrylates / Q000138:chemical synthesis / Q000737:chemistry; D009757:Nylons / Q000138:chemical synthesis / Q000737:chemistry; D010316:Particle Size; D010957:Plasmids / Q000378:metabolism; D000071228:Polyelectrolytes / Q000737:chemistry*; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D014162:Transfection
D008689:Methacrylates; D009757:Nylons; D000071228:Polyelectrolytes; D011091:Polyesters; C407037:poly(2-(dimethylamino)ethyl methacrylate); D011092:Polyethylene Glycols; C033616:poly(lactide); D004247:DNA
DNA plasmid delivery; PEGylation; Quaternised PDMAEMA; Self-assembly; Stimuli-responsive