Functional characterization of the BnNCED3 gene in Brassica napus.
Abscisic acid (ABA) has been implicated in plant adaptation to various environmental stresses and the regulation of seed dormancy, leaf senescence and organ abscission progression. The cleavage of cis-epoxycarotenoids by 9-cis-epoxycarotenoid dioxygenase (NCED) family proteins is a critical step in the regulation of abscisic acid (ABA) synthesis in plants. In the present study, the NCED family gene BnNCED3 was isolated from Brassica napus. BnNCED3 encodes a 592-amino acid protein with high amino acid sequence similarity to the Arabidopsis AtNCED3 protein. Expression pattern assays revealed that BnNCED3 is ubiquitously expressed at different levels in all the examined organs. Furthermore, the overexpression of BnNCED3 contributed to ABA accumulation and NO and ROS generation in transgenic Arabidopsis plants, thereby enhancing abiotic stress tolerance. These experiments also indicated the involvement of BnNCED3 in the control of plant development in transgenic Arabidopsis, such as the inhibition of seed germination, lateral root initiation, early phase changes and the enhancement of ABA-associated leaf senescence. Together, these results indicated that BnNCED3 is at least partly involved in both stress adaptation and plant development through the regulation of ABA biosynthesis.
Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Rd, Shanghai 200032, China. Electronic address: ppxu@sibs.ac.cn.
Peipei
P
Xu
Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Rd, Shanghai 200032, China. Electronic address: wmcai@sibs.ac.cn.
Weiming
W
Cai
D000040:Abscisic Acid / Q000235:genetics* / Q000378:metabolism; D000222:Adaptation, Physiological / Q000235:genetics*; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D017360:Arabidopsis; D029688:Brassica napus / Q000235:genetics*; D049308:Dioxygenases / Q000737:chemistry / Q000235:genetics*; D017343:Genes, Plant; D009569:Nitric Oxide / Q000378:metabolism; D063245:Plant Development / Q000235:genetics*; D010937:Plant Growth Regulators; D018515:Plant Leaves; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D017382:Reactive Oxygen Species / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics*
D000596:Amino Acids; D010937:Plant Growth Regulators; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D000040:Abscisic Acid; D049308:Dioxygenases; C109043:9-cis-epoxy-carotenoid dioxygenase
9-cis-epoxycarotenoid dioxygenase; ABA; Arabidopsis; Brassica napus; Leaf senescence; Stress tolerance