Purification and function of two analgesic and anti-inflammatory peptides from coelomic fluid of the earthworm, Eisenia foetida.
The potential application of anti-inflammatory and analgesic compounds in medication and therapeutic care have become of increasing interest. We purified and characterized two novel analgesic and anti-inflammatory peptides, VQ-5 and AQ-5, from the coelomic fluid of the earthworm (Eisenia foetida). Their primary structures were determined as VSSVQ and AMADQ, respectively. Both peptides, especially AQ-5, exhibited analgesic activity in mouse models of persistent neuropathic pain and inflammation. AQ-5 also inhibited tumor necrosis factor alpha and cyclooxygenase-2 production. The mitogen-activated protein kinase signaling pathway, which is involved in analgesic and anti-inflammatory functions, was inhibited by AQ-5. Thus, the analgesic and anti-inflammatory effects of these peptides, especially AQ-5, demonstrated their potential as candidates for the development of novel analgesic medicines.
Shenzhen Yantian Maternity and Child Health Care Hospital, Shenzhen 518000, Guangdong, China.
Chunlong
C
Li
Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
Mengrou
M
Chen
Faculty of Basic Medical Science, Kunming Medical University, Kunming 650500, Yunnan, China.
Xiaojie
X
Li
Faculty of Basic Medical Science, Kunming Medical University, Kunming 650500, Yunnan, China.
Meifeng
M
Yang
Ethic Drug Screening & Pharmacology Center, Key Laboratory of Chemistry in Ethnic Medicine Resource, State Ethnic Affairs Commission & Ministry of Education, Yunnan Minzu University, Kunming 650500, Yunan, China. Electronic address: wangying_814@163.com.
Ying
Y
Wang
Faculty of Basic Medical Science, Kunming Medical University, Kunming 650500, Yunnan, China. Electronic address: yangxinwanghp@163.com.
Xinwang
X
Yang
D000700:Analgesics / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D001826:Body Fluids / Q000737:chemistry; D004195:Disease Models, Animal; D006801:Humans; D007249:Inflammation / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics; D009437:Neuralgia / Q000188:drug therapy* / Q000235:genetics; D009835:Oligochaeta / Q000737:chemistry; D010455:Peptides / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D015398:Signal Transduction / Q000187:drug effects
D000700:Analgesics; D010455:Peptides; D020928:Mitogen-Activated Protein Kinases
Analgesic; Anti-inflammation; Coelomic fluid; Eisenia foetida; Peptide