Overexpression of persimmon DkXTH1 enhanced tolerance to abiotic stress and delayed fruit softening in transgenic plants.
DkXTH1 promoted cell elongation and more strength to maintain structural integrity by involving in cell wall assembly, thus enhanced tolerance to abiotic stress with broader phenotype in transgenic plants. Xyloglucan endotransglucosylase/hydrolase (XTH) is thought to play a key role in cell wall modifications by cleaving and re-joining xyloglucan, and participates in the diverse physiological processes. DkXTH1 was found to peak in immature expanding persimmon fruit, and its higher expression level exhibited along with firmer fruit during storage. In the present study, transgenic Arabidopsis and tomato plants were generated with DkXTH1 constitutively expressed. Overexpression of DkXTH1 enhanced tolerance to salt, ABA and drought stresses in transgenic Arabidopsis plants with respect to root and leaf growth, and survival. Transgenic tomatoes collected at the mature green stage, presented delayed fruit softening coupled with postponed color change, a later and lower ethylene peak, and higher firmness in comparison with the wild-type tomatoes during storage. Furthermore, broader leaves and tomato fruit with larger diameter were gained in transgenic Arabidopsis and tomato, respectively. Most importantly, transgenic plants exhibited more large and irregular cells with higher density of cell wall and intercellular spaces, resulting from the overactivity of XET enzymes involving in cell wall assembly. We suggest that DkXTH1 expression resulted in cells with more strength and thickness to maintain structural integrity, and thus enhanced tolerance to abiotic stress and delayed fruit softening in transgenic plants.
College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Ye
Y
Han
College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Shoukun
S
Han
College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Qiuyan
Q
Ban
College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Yiheng
Y
He
College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Mijing
M
Jin
College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China. hanyest1989@163.com.
Jingping
J
Rao
D017360:Arabidopsis / Q000235:genetics; D029788:Diospyros / Q000235:genetics*; D005638:Fruit / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D016695:Glycosyltransferases / Q000235:genetics* / Q000378:metabolism; D018551:Solanum lycopersicum / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000502:physiology; D013312:Stress, Physiological / Q000235:genetics*
D010940:Plant Proteins; D016695:Glycosyltransferases; C473049:xyloglucan - xyloglucosyltransferase
Abiotic stress; Cell elongation; Cell wall; Fruit softening; Xyloglucan endotransglucosylase/hydrolase