Polyvinyl alcohol composite nanofibres containing conjugated levofloxacin-chitosan for controlled drug release.
A range of biodegradable drug-nanofibres composite mats have been reported as drug delivery systems. However, their main disadvantage is the rapid release of the drug immediately after application. This paper reports an improved system based on the incorporation of drug conjugated-chitosan into polyvinyl alcohol (PVA) nanofibers. The results showed that controlled release of levofloxacin (LVF) could be achieved by covalently binding LVF to low molecular weight chitosan (CS) via a cleavable amide bond and then blending the conjugated CS with polyvinyl alcohol (PVA) nanofibres prior to electrospinning. PVA/LVF and PVA-CS/LVF nanofibres were fabricated as controls. The conjugated CS-LVF was characterized by FTIR, DSC, TGA and1H NMR. Scanning electron microscopy (SEM) showed that the blended CS-PVA nanofibres had a reduced fibre diameter compared to the controls. Drug release profiles showed that burst release was decreased from 90% in the control PVA/LVF electrospun mats to 27% in the PVA/conjugated CS-LVF mats after 8h in phosphate buffer at 37°C. This slower release is due to the cleavable bond between LVF and CS that slowly hydrolysed over time at neutral pH. The results indicate that conjugation of the drug to the polymer backbone is an effective way of minimizing burst release behaviour and achieving sustained release of the drug, LVF.
CSIRO, Manufacturing Flagship, Bayview Ave, Clayton, Victoria 3168, Australia; School of Fashion and Textiles, College of Design and Social Context, RMIT University, 25 Dawson Street, Brunswick, Victoria 3056, Australia. Electronic address: Javid.jlv@gmail.com.
Javid
J
Jalvandi
School of Fashion and Textiles, College of Design and Social Context, RMIT University, 25 Dawson Street, Brunswick, Victoria 3056, Australia. Electronic address: tamrak@bigpond.com.
Max
M
White
CSIRO, Manufacturing Flagship, Bayview Ave, Clayton, Victoria 3168, Australia. Electronic address: Yuan.Gao@csiro.au.
Yuan
Y
Gao
CSIRO, Manufacturing Flagship, Bayview Ave, Clayton, Victoria 3168, Australia. Electronic address: Yen.truong@csiro.au.
Yen Bach
YB
Truong
School of Fashion and Textiles, College of Design and Social Context, RMIT University, 25 Dawson Street, Brunswick, Victoria 3056, Australia. Electronic address: rajiv.padhye@rmit.edu.au.
Rajiv
R
Padhye
CSIRO, Manufacturing Flagship, Bayview Ave, Clayton, Victoria 3168, Australia. Electronic address: Louis.kyratzis@csiro.au.
Ilias Louis
IL
Kyratzis
D002152:Calorimetry, Differential Scanning; D048271:Chitosan / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000494:pharmacology; D064704:Levofloxacin / Q000494:pharmacology*; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry
D003692:Delayed-Action Preparations; D064704:Levofloxacin; D011142:Polyvinyl Alcohol; D048271:Chitosan
Chitosan; Conjugation; Controlled release; Electrospinning; Levofloxacin; Polyvinyl alcohol