A Selective Cell Population from Dermis Strengthens Bone Regeneration.
Finding appropriate seed cells for bone tissue engineering remains a significant challenge. Considering that skin is the largest organ, we hypothesized that human bone morphogenetic protein receptor type IB (BmprIB)+ dermal cells could have enhanced osteogenic capacity in the healing of critical-sized calvarial defects in an immunodeficient mouse model. In this study, immunohistochemical staining revealed that BmprIB was expressed throughout reticular dermal cells; the positive expression rate of BmprIB was 3.5% ± 0.4% in freshly separated dermal cells, by flow cytometry. Furthermore, in vitro osteogenic capacity of BmprIB+ cells was confirmed by osteogenic-related staining and marker gene expression compared with unsorted dermal cells. In vivo osteogenic capacity was demonstrated by implantation of human BmprIB+ cell/coral constructs in the treatment of 4-mm diameter calvarial defects in an immunodeficient mouse model compared with implantation of unsorted cell/coral constructs and coral scaffold alone. These results indicate that the selective cell population BmprIB from human dermis is a promising osteogenic progenitor cell that can be a large-quantity and high-quality cell source for bone tissue engineering and regeneration. Stem Cells Translational Medicine 2017;6:306-315.
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital Affiliated with Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
Tingliang
T
Wang
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital Affiliated with Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
Jinguang
J
He
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital Affiliated with Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
Yang
Y
Zhang
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital Affiliated with Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
Wenjun
W
Shi
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital Affiliated with Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
Jiasheng
J
Dong
Stem Cell and Tissue Engineering Laboratory, Department of Orthopaedics, and Division of Exercise Physiology, West Virginia University, Morgantown, West Virginia, USA.
Ming
M
Pei
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital Affiliated with Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
Lian
L
Zhu
D000818:Animals; D052005:Bone Morphogenetic Protein Receptors, Type I / Q000378:metabolism; D001861:Bone Regeneration / Q000502:physiology*; D002648:Child; D002675:Child, Preschool; D020405:Dermis / Q000166:cytology*; D006801:Humans; D007223:Infant; D008297:Male; D051379:Mice; D010012:Osteogenesis; D054457:Tissue Scaffolds / Q000737:chemistry; D014057:Tomography, X-Ray Computed
C498214:BMPR1B protein, human; D052005:Bone Morphogenetic Protein Receptors, Type I
Bone morphogenetic protein receptor type IB; Bone tissue engineering; Calvarial defect; Coral; Skin