A highly sensitive and selective fluorescence off-on probe for the detection of intracellular endogenous tyrosinase activity.
A resorufin-based highly sensitive and selective fluorescence off-on probe with a new recognition moiety for tyrosinase is developed, and applied to detect and image endogenous tyrosinase activity in different living cells.
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. lixh@iccas.ac.cn mahm@iccas.ac.cn and University of the Chinese Academy of Sciences, Beijing 100049, China.
Xiaofeng
X
Wu
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. lixh@iccas.ac.cn mahm@iccas.ac.cn and University of the Chinese Academy of Sciences, Beijing 100049, China.
Xiaohua
http://orcid.org/0000-0001-5656-4444
X
Li
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. lixh@iccas.ac.cn mahm@iccas.ac.cn.
Hongyu
H
Li
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. lixh@iccas.ac.cn mahm@iccas.ac.cn.
Wen
http://orcid.org/0000-0002-2368-3997
W
Shi
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. lixh@iccas.ac.cn mahm@iccas.ac.cn and University of the Chinese Academy of Sciences, Beijing 100049, China.
Huimin
H
Ma
D000818:Animals; D045744:Cell Line, Tumor; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D014442:Monophenol Monooxygenase / Q000032:analysis*; D010078:Oxazines / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D013696:Temperature
D005456:Fluorescent Dyes; D010078:Oxazines; D014442:Monophenol Monooxygenase