Comprehensive Analysis of Rice Laccase Gene (OsLAC) Family and Ectopic Expression of OsLAC10 Enhances Tolerance to Copper Stress in Arabidopsis.
Laccases are encoded by a multigene family and widely distributed in plant genomes where they play roles oxidizing monolignols to produce higher-order lignin involved in plant development and stress responses. We identified 30 laccase genes (OsLACs) from rice, which can be divided into five subfamilies, mostly expressed during early development of the endosperm, growing roots, and stems. OsLACs can be induced by hormones, salt, drought, and heavy metals stresses. The expression level of OsLAC10 increased 1200-fold after treatment with 20 μM Cu for 12 h. The laccase activities of OsLAC10 were confirmed in an Escherichia coli expression system. Lignin accumulation increased in the roots of Arabidopsis over-expressing OsLAC10 (OsLAC10-OX) compared to wild-type controls. After growth on 1/2 Murashige and Skoog (MS) medium containing toxic levels of Cu for seven days, roots of the OsLAC10-OX lines were significantly longer than those of the wild type. Compared to control plants, the Cu concentration decreased significantly in roots of the OsLAC10-OX line under hydroponic conditions. These results provided insights into the evolutionary expansion and functional divergence of OsLAC family. In addition, OsLAC10 is likely involved in lignin biosynthesis, and reduces the uptake of Cu into roots required for Arabidopsis to develop tolerance to Cu.
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China. liuqingquan@cnbg.net.|Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China. liuqingquan@cnbg.net.
Qingquan
Q
Liu
College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China. luole@njau.edu.cn.
Le
L
Luo
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China. 2014116043@njau.edu.cn.
Xiaoxiao
X
Wang
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China. zgshen@njau.edu.cn.
Zhenguo
Z
Shen
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China. zhenglq@njau.edu.cn.
Luqing
L
Zheng
D000220:Adaptation, Biological / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D001692:Biological Transport; D016000:Cluster Analysis; D003300:Copper / Q000378:metabolism*; D000066630:Ectopic Gene Expression; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D042845:Laccase / Q000235:genetics*; D005810:Multigene Family; D012275:Oryza / Q000235:genetics*; D010802:Phylogeny; D030821:Plants, Genetically Modified; D013312:Stress, Physiological / Q000235:genetics
D003300:Copper; D042845:Laccase
Arabidopsis; OsLAC10; copper tolerance; copper uptake; rice laccase