Albumin-mediated deposition of bone-like apatite onto nano-sized surfaces: Effect of surface reactivity and interfacial hydration.
The bioactivity of an implant is displayed on its ability to induce heterogeneous nucleation of biogenic apatite onto its surface upon immersion in body fluids; forming, through this layer, a stable bond with the host tissue. The present article evaluates the bioactivity of different nanostructured substrates based on synthetic hydroxyapatite (HA) and titania (TiO2) nanoparticles, where we extend the debate regarding the selective roles played by the presence of albumin on the biogenic apatite coating evolution. The substrates bone-bonding potential was evaluated by keeping the materials in contact with Simulated Body Fluid, while the influence of the presence of Bovine Serum Albumin in bioactivity was analyzed by a spectrophotometric technique. Our results show that materials' surface reactivity and their interfacial hydration are responsible for the bonding-site alteration and surface charge density distribution, which in turn, regulate the protein adsorption process. As a matter of fact, variations on the protein adsorbed density have a directly proportional impact on calcium binding sites, which should be responsible for the initiation of the mineralization process, disturbing the deposition of the interfacial calcium phosphate (Ca-P) mineralized coating.
Department of Chemistry, Universidad Nacional del Sur, INQUISUR-CONICET, B8000CPB Bahía Blanca, Argentina. Electronic address: nldelia@inquisur-conicet.gob.ar.
Noelia L
NL
D'Elia
Department of Chemistry, Universidad Nacional del Sur, INQUISUR-CONICET, B8000CPB Bahía Blanca, Argentina. Electronic address: noel.gravina@uns.edu.ar.
Noel
N
Gravina
Soft Matter and Molecular Biophysics Group, Department of Applied Physics, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain. Electronic address: juanm.ruso@usc.es.
Juan M
JM
Ruso
Environmental Research and Engineering Institute, CONICET, Universidad de San Martín, B1650HMP San Martín, Argentina. Electronic address: josemarcobrown@gmail.com.
Jose L
JL
Marco-Brown
Instituto de Ingeniería Electroquímica y Corrosión, INIEC-CONICET, Universidad Nacional del Sur, B8000CPB Bahía Blanca, Argentina. Electronic address: jmsieben@uns.edu.ar.
Juan M
JM
Sieben
Department of Chemistry, Universidad Nacional del Sur, INQUISUR-CONICET, B8000CPB Bahía Blanca, Argentina. Electronic address: pmessina@uns.edu.ar.
Paula V
PV
Messina
D000327:Adsorption; D001826:Body Fluids / Q000737:chemistry; D001842:Bone and Bones / Q000737:chemistry*; D002113:Calcification, Physiologic; D002130:Calcium Phosphates / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry; D014867:Water / Q000737:chemistry
D002130:Calcium Phosphates; D014867:Water; D012710:Serum Albumin, Bovine; D017886:Durapatite; C020243:calcium phosphate; D014025:Titanium
Adsorption kinetics; Bovine serum albumin; Hydroxyapatite; Titania