Synthesis, Characterization and Luminescence Sensitivity with Variance in pH, DNA and BSA Binding Studies of Ru(II) Polypyridyl Complexes.
Three ruthenium(II) polypyridyl complexes, [Ru(phen)2(mip)](ClO4)2(1) (phen =1,10-Phenanthroline), [Ru(bpy)2(mip)](ClO4)2(2) (bpy = 2,2'bipyridyl) and [Ru(dmb)2(mip)](ClO4)2(3) (dmb = 4, 4'-dimethyl 2, 2'-bipyridine), were synthesized with an intercalative ligand mip (2-morpholino-1H-imidazo[4,5-f][1, 10]phenanthroline) and characterized by1H,13C-NMR, IR, UV-vis, mass spectra and elemental analysis. pH effect, ion selectivity (cations, anions) and solvent sensitivity of complexes were studied. The interaction of these complexes with DNA was performed using absorption, emission spectroscopy and viscosity measurements. The experimental results indicated that the two complexes interacted with calf thymus DNA (CT-DNA) by intercalative mode. BSA (Bovine Serum Albumin) protein binding of these complexes was studied by UV-visible and fluorescence techniques. The binding capacity of these complexes was explained theoretically by molecular docking method.
Department of Chemistry, Osmania University, Hyderabad, Telangana State, 500007, India.
Ravi Kumar
RK
Vuradi
Department of Chemistry, Osmania University, Hyderabad, Telangana State, 500007, India.
Srishailam
S
Avudoddi
Department of Chemistry, Osmania University, Hyderabad, Telangana State, 500007, India.
Venkat Reddy
VR
Putta
Department of Chemistry, Osmania University, Hyderabad, Telangana State, 500007, India.
Laxma Reddy
LR
Kotha
Department of Chemistry, Osmania University, Hyderabad, Telangana State, 500007, India.
Praveen Kumar
PK
Yata
Department of Chemistry, Osmania University, Hyderabad, Telangana State, 500007, India. ssnsirasani@gmail.com.
Satyanarayana
S
Sirasani
D015082:2,2'-Dipyridyl / Q000737:chemistry*; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007364:Intercalating Agents / Q000737:chemistry / Q000378:metabolism; D049449:Luminescence; D062105:Molecular Docking Simulation; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism*; D012428:Ruthenium / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*
D007364:Intercalating Agents; D009942:Organometallic Compounds; D012710:Serum Albumin, Bovine; D015082:2,2'-Dipyridyl; D012428:Ruthenium; D004247:DNA; C427203:calf thymus DNA
BSA binding; DNA binding; Docking studies; Ru(II) complexes; Sensor effect; pH effect