IL-17B activated mesenchymal stem cells enhance proliferation and migration of gastric cancer cells.
Mesenchymal stem cells are important cells in tumor microenvironment. We have previously demonstrated that IL-17B/IL-17RB signal promoted progression of gastric cancer. In this study, we further explored the effect of IL-17B on mesenchymal stem cells in tumor microenvironment and its impact on the tumor progression. The results showed that IL-17B induced the expression of stemness-related genes Nanog, Sox2, and Oct4 in mesenchymal stem cells and enhanced its tumor-promoting effect. The supernatant from cultured mesenchymal stem cells after treating with exogenous rIL-17B promoted the proliferation and migration of MGC-803, therefor suggesting that rIL-17B might promote mesenchymal stem cells to produce soluble factors. In addition, rIL-17B also activated the NF-κΒ, STAT3, β-catenin pathway in mesenchymal stem cells. Our data revealed a new mechanism that IL-17B enhanced the progression of gastric cancer by activating mesenchymal stem cells.
Department of Immunology, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China.
Qingli
Q
Bie
Department of Immunology, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China.
Bin
B
Zhang
Department of Anesthesiology, The Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, China.
Caixia
C
Sun
Department of Immunology, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China.
Xiaoyun
X
Ji
Department of Biomedical and Forensic Sciences, School of Biological Sciences, University of Cape Coast, Cape Coast, Ghana.
Prince Amoah
PA
Barnie
Department of Immunology, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China.
Chen
C
Qi
Department of Immunology, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China.
Jingjing
J
Peng
Department of Immunology, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China.
Danyi
D
Zhang
Department of Immunology, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China.
Dong
D
Zheng
Department of Immunology, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China.
Zhaoliang
Z
Su
Department of Immunology, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China.|Key Laboratory of Laboratory Medicine of Jiangsu Province, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China.
Shengjun
S
Wang
Department of Immunology, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China.|Key Laboratory of Laboratory Medicine of Jiangsu Province, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China.
Huaxi
H
Xu
D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D006801:Humans; D020381:Interleukin-17 / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000473:pathology*; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000473:pathology*; D059016:Tumor Microenvironment / Q000502:physiology*
D020381:Interleukin-17
IL-17B; gastric cancer; mesenchymal stem cells (MSCs); stemness