Molecular characterization of the capsular antigens ofPasteurella multocidaisolates using multiplex PCR.
The use of molecular techniques for detection and characterization of thePasteurella multocidais very important for rapid and specific detection and characterization of the organism. During the period from 15th February, 2014 to 15th April, 2015, 425 nasopharyngeal swabs and 175 lung and spleen samples were collected and examined by conventional methods, 80 strains (18.82%) ofP. multocidawere isolated from the calves, sheep and goat with respiratory manifestation. Meanwhile, 77 strains (44%) were isolated from emergency slaughtered animals. All the recovered strains were positive for specific PCR for detection ofP. multocidastrains previously identified asP. multocidaby standard microbiological techniques. Multiplex PCR for molecular typing of the capsular antigens of the recoveredP. multocidarevealed positive amplification of 1044 bp fragments specific to the capsular antigen type A with 105 strains (66.88%), and amplification 511 bp fragments of the capsular antigen type E with 52 strain (33.12%) and absence of B, D and F antigens. Multiplex PCR for molecular typing of the capsular antigens ofP. multocidacan be used as a simple, sensitive, rapid, reliable technique instead of the serological techniques for identification of the capsular antigens ofP. multocida.
Department of Botany and Microbiology, College of Science, King Saud University, Saudi Arabia.
Khalid S
KS
Al-Maary
Department of Botany and Microbiology, College of Science, King Saud University, Saudi Arabia.
Turki M
TM
Dawoud
Department of Botany and Microbiology, College of Science, King Saud University, Saudi Arabia.
Ayman S
AS
Mubarak
Department of Health Science, College of Applied Studies and Community Service, King Saud University, P.O. Box 22459, Riyadh 11495, Saudi Arabia; Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, P.O. 2446, Cairo, 14242 Giza, Egypt.
Ashgan M
AM
Hessain
Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, P.O. 2446, Cairo, 14242 Giza, Egypt.
Hussein M
HM
Galal
Department of Biology, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah, Saudi Arabia.
Saleh A
SA
Kabli
Department of Botany and Microbiology, College of Science, King Saud University, Saudi Arabia; Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, P.O. 2446, Cairo, 14242 Giza, Egypt.
Moussa I
MI
Mohamed
Capsular antigens; Microbiological techniques; Molecular typing; Multiplex PCR; P. multocida