Acute exercise elicits differential expression of insulin resistance genes in the skeletal muscle of patients with polycystic ovary syndrome.
OBJECTIVE
This study aimed to explore the role of acute exercise on skeletal muscle gene expression related to insulin resistance in patients with polycystic ovary syndrome (PCOS) and controls.


METHODS
Four obese women with PCOS and four body mass index (BMI)-matched controls (CTRL) participated in this study. After an overnight fast, the subjects underwent a single 40-min bout of aerobic exercise. Muscle samples were obtained from vastus lateralis at baseline and 60 min after exercise. The expression of a panel of insulin resistance genes was evaluated by a quantitative PCR array system. Network-based analyses were performed to interpret transcriptional changes occurring before and after the exercise challenge.


RESULTS
Overall, differentially expressed genes associated with mitochondria function and peroxisome proliferator-activated receptor signalling were identified. At baseline, there was a significant upregulation of six genes exclusively in PCOS (i.e. NFKBIA, MAPK3, PPARGC1A, GAPDH, ACTB and PPARA). Twelve genes were upregulated in CTRL after a single bout of aerobic exercise (i.e. LEPR, CXCR4, CCR5, IL-18R1, CRLF2, ACACA, CEBPA, PPARGC1A, UCP1, TNFRSF1B, TLR4 and IKBKB). After the exercise session, three genes were upregulated in PCOS (i.e. SOCS3, NAMPT and IL-8), whilst IL-6 was upregulated in both groups after exercise.


CONCLUSIONS
This study provides novel evidence on the effects of acute exercise on insulin resistance genes in skeletal muscle of PCOS. The differentially expressed genes reported herein could be further investigated as targets for therapeutic interventions aimed at improving insulin resistance in this syndrome.
School of Physical Education and Sport, University of Sao Paulo, Sao Paulo, Brazil.
Wagner Silva
http://orcid.org/0000-0003-4561-4930
WS
Dantas
School of Physical Education and Sport, University of Sao Paulo, Sao Paulo, Brazil.
Igor Hisashi
IH
Murai
Laboratory of Transplantation Immunobiology, Department of Immunology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil.
Luiz Augusto
LA
Perandini
Department of Pediatrics, School of Medicine, University of Sao Paulo, Sao Paulo, Brazil.
Hatylas
H
Azevedo
Department of Pediatrics, School of Medicine, University of Sao Paulo, Sao Paulo, Brazil.
Carlos Alberto
CA
Moreira-Filho
Laboratory of Transplantation Immunobiology, Department of Immunology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil.
Niels Olsen Saraiva
NO
Camara
School of Physical Education and Sport, University of Sao Paulo, Sao Paulo, Brazil.|Rheumatology Division, School of Medicine, University of Sao Paulo, Sao Paulo, Brazil.
Hamilton
H
Roschel
School of Physical Education and Sport, University of Sao Paulo, Sao Paulo, Brazil.|Rheumatology Division, School of Medicine, University of Sao Paulo, Sao Paulo, Brazil.
Bruno
B
Gualano
D000328:Adult; D005081:Exercise Therapy / Q000379:methods*; D005260:Female; D015870:Gene Expression / Q000235:genetics*; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics*; D018482:Muscle, Skeletal / Q000378:metabolism*; D009765:Obesity / Q000628:therapy*; D011085:Polycystic Ovary Syndrome / Q000628:therapy*; D055815:Young Adult