Improved Extraction and Quality Characterization of Water-Soluble Polysaccharide from Gamma-Irradiated Lentinus edodes.
Gamma irradiation was applied to the improved extraction of water-soluble polysaccharides (WSPs) from dried Lentinus edodes. Irradiation provided a dose-dependent increase in extraction yield (0 kGy, 2.01%; 7.5 kGy, 4.03%; 15 kGy, 7.17%) and purity (0 kGy, 78.8%; 7.5 kGy, 83.1%; 15 kGy, 85.6%) of the WSPs from hot-water extraction. The effect of irradiation was evident in the degraded microstructures and reduced molecular weights of the WSPs. However, nuclear magnetic resonance, Fourier-transform infrared, and X-ray diffraction spectroscopic analyses provided comparable structures of WSPs from nonirradiated and irradiated samples. UV-visible spectra showed a dose-dependent decline in intensity, but an improvement in thermal properties of the WSPs from the irradiated mushroom samples was observed.
School of Food Science & Biotechnology, Kyungpook Natl. Univ., Daegu, 702-701, Republic of Korea.|Inst. of Food Science & Nutrition, Bahauddin Zakariya Univ., Multan, Pakistan.
Kashif
K
Akram
School of Food Science & Biotechnology, Kyungpook Natl. Univ., Daegu, 702-701, Republic of Korea.|Dept. of Biotechnology, Yonsei Univ., Seoul, 03722, Republic of Korea.
Hafiz Muhammad
HM
Shahbaz
School of Food Science & Biotechnology, Kyungpook Natl. Univ., Daegu, 702-701, Republic of Korea.
Gui-Ran
GR
Kim
Inst. of Food Science & Nutrition, Bahauddin Zakariya Univ., Multan, Pakistan.|Inst. of Food Science & Nutrition, Univ. of Sargodha, Sargodha, 40100, Pakistan.
Umar
U
Farooq
School of Food Science & Biotechnology, Kyungpook Natl. Univ., Daegu, 702-701, Republic of Korea.
Joong-Ho
JH
Kwon
D005720:Gamma Rays; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D020942:Shiitake Mushrooms / Q000737:chemistry* / Q000254:growth & development / Q000528:radiation effects; D014675:Vegetables / Q000737:chemistry*; D014961:X-Ray Diffraction
D010936:Plant Extracts; D011134:Polysaccharides
Lentinus edodes; extraction; gamma irradiation; water-soluble polysaccharide