Isolation and characterization of a novel pollen-specific promoter in maize (Zea mays L.).
ZmSTK2_USP, located on the long arm of chromosome 4, belongs to the serine/threonine kinase gene in maize. The sequence analysis of 2100 bp upstream from the start codon ATG has shown that it contains cis-element motifs and two types of anther/pollen-specific promoter elements (GTGA and AGAAA), suggesting that it is the pollen-specific promoter. To investigate the function of ZmSTK2_USP promoter, the GUS gene fusion system was employed. In proZmSTK2_USP-GUS genetically modified plants, GUS activity was detected in mature pollen grains and pollen tubes but not found in other floral and vegetative tissues. These results show that proZmSTK2_USP is the pollen-specific promoter and drives pollen-specific activity during the middle stage of pollen development until pollen maturation.
a Agronomy College, Shenyang Agricultural University, No. 120 Dongling Road, Shenyang, Liaoning 110866, China.|b Biotechnology and Bioscience College, Shenyang Agricultural University, No. 120 Dongling Road, Shenyang, Liaoning 110866, China.|c Corn Research Institute, Liaoning Academy of Agricultural Sciences, No. 84 Dongling Road, Shenyang, Liaoning 110866, China.
He
H
Wang
a Agronomy College, Shenyang Agricultural University, No. 120 Dongling Road, Shenyang, Liaoning 110866, China.|b Biotechnology and Bioscience College, Shenyang Agricultural University, No. 120 Dongling Road, Shenyang, Liaoning 110866, China.
Mingxia
M
Fan
c Corn Research Institute, Liaoning Academy of Agricultural Sciences, No. 84 Dongling Road, Shenyang, Liaoning 110866, China.
Guohong
G
Wang
b Biotechnology and Bioscience College, Shenyang Agricultural University, No. 120 Dongling Road, Shenyang, Liaoning 110866, China.
Chunyu
C
Zhang
c Corn Research Institute, Liaoning Academy of Agricultural Sciences, No. 84 Dongling Road, Shenyang, Liaoning 110866, China.
Lei
L
Shi
d Laboratory of Plant Bioreactor and Genetics Engineering, Jilin Provincial Key Laboratory of Agricultural Biotechnology, Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, No. 1363 Shengtai St., Changchun 130033, China.
Zhengyi
Z
Wei
b Biotechnology and Bioscience College, Shenyang Agricultural University, No. 120 Dongling Road, Shenyang, Liaoning 110866, China.
Wenjuan
W
Ma
b Biotechnology and Bioscience College, Shenyang Agricultural University, No. 120 Dongling Road, Shenyang, Liaoning 110866, China.
Jing
J
Chang
b Biotechnology and Bioscience College, Shenyang Agricultural University, No. 120 Dongling Road, Shenyang, Liaoning 110866, China.
Senxin
S
Huang
b Biotechnology and Bioscience College, Shenyang Agricultural University, No. 120 Dongling Road, Shenyang, Liaoning 110866, China.
Feng
F
Lin
D001483:Base Sequence; D032461:Chromosomes, Plant / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics; D011058:Pollen / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
D018744:DNA, Plant; D010940:Plant Proteins
GUS activity; PCR; ZmSTK2_USP gene; activité GUS; gène ZmSTK2_USP; maize; maïs; pollen-specific promoter; promoteur pollen-spécifique