Effects of T-2 Toxin on Pacific White Shrimp Litopenaeus vannamei: Growth, and Antioxidant Defenses and Capacity and Histopathology in the Hepatopancreas.
Modified-masked T-2 toxin (mT-2) formed during metabolism in edible aquatic animals may go undetected by traditional analytical methods, thereby underestimating T-2 toxicity. The effects of T-2 on growth and antioxidant capacity and histopathological changes in the hepatopancreas were studied in Pacific white shrimp Litopenaeus vannamei exposed for 20 d to 0, 0.5, 1.2, 2.4, 4.8, and 12.2 mg/kg of T-2 in their feed. The concentration of mT-2 in the hepatopancreas was detected by liquid chromatography-tandem mass spectrophotometry before and after trifluoroacetic acid (TFA) treatment that converted mT-2 to free T-2. A dose-dependent increase in mT-2 concentration was observed in the hepatopancreas. Dietary exposure to T-2 significantly decreased (P < 0.05) shrimp growth and survival rate compared with the controls. The malondialdehyde (MDA) concentration was significantly increased in shrimp exposed to feed with ≥2.4 mg/kg T-2 (P < 0.05). The antioxidant enzymes, superoxide dismutase (SOD) and glutathione peroxidase (GPx), total antioxidant capacity (T-AOC), and also glutathione (GSH) content increased in shrimp dosed with 2.4-4.8 mg/kg T-2 but declined at the highest dose (12.2 mg/kg), probably indicating an inability to cope with high concentrations of reactive oxygen species (ROS) as evident from a marked increase in MDA (P < 0.05) culminating in cellular toxicity. Histopathological changes in the hepatopancreas were dose dependent, with cell autophagy evident at the highest exposure dose. This is the first report in shrimp of a dose-dependent increase in ROS, SOD enzyme activity, and T-AOC at low T-2 exposures, and associated histopathological changes in the hepatopancreas, in response to dietary T-2. Received January 26, 2016; accepted October 9, 2016.
a College of Food Science and Technology, Guangdong Ocean University , Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution , Zhanjiang 524088 , China.
Yijia
Y
Deng
a College of Food Science and Technology, Guangdong Ocean University , Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution , Zhanjiang 524088 , China.
Yaling
Y
Wang
a College of Food Science and Technology, Guangdong Ocean University , Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution , Zhanjiang 524088 , China.
Xiaodi
X
Zhang
a College of Food Science and Technology, Guangdong Ocean University , Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution , Zhanjiang 524088 , China.
Lijun
L
Sun
a College of Food Science and Technology, Guangdong Ocean University , Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution , Zhanjiang 524088 , China.
Chaojin
C
Wu
a College of Food Science and Technology, Guangdong Ocean University , Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution , Zhanjiang 524088 , China.
Qi
Q
Shi
a College of Food Science and Technology, Guangdong Ocean University , Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution , Zhanjiang 524088 , China.
Rundong
R
Wang
b College of Environment and Resources , Dalian Nationalities University , Dalian , 116600 , China.
Xiaodong
X
Sun
c Shenzhen Bioeasy Biotechnologies Company Ltd ., Shenzhen , 518102 , China.
Siyuan
S
Bi
d Centre for Food Research and Innovation, Department of Wine, Food and Molecular Biosciences , Lincoln University , Lincoln 7647 , Canterbury , New Zealand.
Ravi
R
Gooneratne
D000818:Animals; D043143:Hepatopancreas / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress; D033561:Penaeidae / Q000187:drug effects* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013605:T-2 Toxin / Q000494:pharmacology*
D013482:Superoxide Dismutase; D013605:T-2 Toxin