The Transcription Factor MYB29 Is a Regulator ofALTERNATIVE OXIDASE1a.
Plants sense and integrate a variety of signals from the environment through different interacting signal transduction pathways that involve hormones and signaling molecules. UsingALTERNATIVE OXIDASE1a(AOX1a) gene expression as a model system of retrograde or stress signaling between mitochondria and the nucleus, MYB DOMAIN PROTEIN29 (MYB29) was identified as a negative regulator (regulator of alternative oxidase1a 7[rao7] mutant) in a genetic screen of Arabidopsis (Arabidopsis thaliana).rao7/myb29mutants have increased levels of AOX1a transcript and protein compared to wild type after induction with antimycin A. A variety of genes previously associated with the mitochondrial stress response also display enhanced transcript abundance, indicating that RAO7/MYB29 negatively regulates mitochondrial stress responses in general. Meta-analysis of hormone-responsive marker genes and identification of downstream transcription factor networks revealed that MYB29 functions in the complex interplay of ethylene, jasmonic acid, salicylic acid, and reactive oxygen species signaling by regulating the expression of various ETHYLENE RESPONSE FACTOR and WRKY transcription factors. Despite an enhanced induction of mitochondrial stress response genes,rao7/myb29mutants displayed an increased sensitivity to combined moderate light and drought stress. These results uncover interactions between mitochondrial retrograde signaling and the regulation of glucosinolate biosynthesis, both regulated by RAO7/MYB29. This common regulator can explain why perturbation of the mitochondrial function leads to transcriptomic responses overlapping with responses to biotic stress.
Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China (X.Z).|Department of Animal, Plant, and Soil Science, Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, Bundoora, Victoria 3086, Australia (X.Z., A.I., J.R., O.B., Y.X., B.Z., J.W., I.D.C.).
Xinhua
0000-0002-6045-5010
X
Zhang
Department of Animal, Plant, and Soil Science, Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, Bundoora, Victoria 3086, Australia (X.Z., A.I., J.R., O.B., Y.X., B.Z., J.W., I.D.C.).
Aneta
0000-0002-2465-7213
A
Ivanova
Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium (K.V., J.V.d.V., P.W., V.S., D.V., F.V.B., I.D.C.).|Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (K.V., J.V.d.V., P.W., V.S., D.V., F.V.B., I.D.C.).
Klaas
0000-0003-4790-2725
K
Vandepoele
Department of Animal, Plant, and Soil Science, Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, Bundoora, Victoria 3086, Australia (X.Z., A.I., J.R., O.B., Y.X., B.Z., J.W., I.D.C.).
Jordan
J
Radomiljac
Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium (K.V., J.V.d.V., P.W., V.S., D.V., F.V.B., I.D.C.).|Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (K.V., J.V.d.V., P.W., V.S., D.V., F.V.B., I.D.C.).
Jan
0000-0001-7742-1266
J
Van de Velde
Department of Animal, Plant, and Soil Science, Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, Bundoora, Victoria 3086, Australia (X.Z., A.I., J.R., O.B., Y.X., B.Z., J.W., I.D.C.).
Oliver
0000-0002-7671-6983
O
Berkowitz
Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium (K.V., J.V.d.V., P.W., V.S., D.V., F.V.B., I.D.C.).|Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (K.V., J.V.d.V., P.W., V.S., D.V., F.V.B., I.D.C.).|Medical Biotechnology Center, VIB, 9000 Ghent, Belgium (P.W.).|Department of Biochemistry, Ghent University, 9000 Ghent, Belgium (P.W.).
Patrick
0000-0003-4667-2294
P
Willems
Department of Animal, Plant, and Soil Science, Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, Bundoora, Victoria 3086, Australia (X.Z., A.I., J.R., O.B., Y.X., B.Z., J.W., I.D.C.).
Yue
0000-0002-4615-5441
Y
Xu
Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (S.N., O.V.A., O.D.); and.
Sophia
0000-0002-1677-863X
S
Ng
Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (S.N., O.V.A., O.D.); and.
Olivier
0000-0003-4024-968X
O
Van Aken
Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (S.N., O.V.A., O.D.); and.
Owen
O
Duncan
Department of Animal, Plant, and Soil Science, Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, Bundoora, Victoria 3086, Australia (X.Z., A.I., J.R., O.B., Y.X., B.Z., J.W., I.D.C.).
Botao
B
Zhang
Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium (K.V., J.V.d.V., P.W., V.S., D.V., F.V.B., I.D.C.).|Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (K.V., J.V.d.V., P.W., V.S., D.V., F.V.B., I.D.C.).
Veronique
0000-0003-4762-6580
V
Storme
Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, Australian National University, Acton, Australian Capital Territory 2601, Australia (K.X.C., B.J.P.).
Kai Xun
0000-0003-3554-7228
KX
Chan
Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium (K.V., J.V.d.V., P.W., V.S., D.V., F.V.B., I.D.C.).|Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (K.V., J.V.d.V., P.W., V.S., D.V., F.V.B., I.D.C.).
Dries
0000-0002-8975-2801
D
Vaneechoutte
Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, Australian National University, Acton, Australian Capital Territory 2601, Australia (K.X.C., B.J.P.).
Barry James
0000-0003-1869-2423
BJ
Pogson
Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium (K.V., J.V.d.V., P.W., V.S., D.V., F.V.B., I.D.C.).|Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (K.V., J.V.d.V., P.W., V.S., D.V., F.V.B., I.D.C.).
Frank
0000-0002-3147-0860
F
Van Breusegem
Department of Animal, Plant, and Soil Science, Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, Bundoora, Victoria 3086, Australia (X.Z., A.I., J.R., O.B., Y.X., B.Z., J.W., I.D.C.).
James
0000-0001-5754-025X
J
Whelan
Department of Animal, Plant, and Soil Science, Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, Bundoora, Victoria 3086, Australia (X.Z., A.I., J.R., O.B., Y.X., B.Z., J.W., I.D.C.).|Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium (K.V., J.V.d.V., P.W., V.S., D.V., F.V.B., I.D.C.).|Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (K.V., J.V.d.V., P.W., V.S., D.V., F.V.B., I.D.C.).
Inge
0000-0001-8125-1239
I
De Clercq
D000968:Antimycin A / Q000494:pharmacology; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics*; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D015151:Immunoblotting; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D000099188:Alternative Oxidase
D000968:Antimycin A; D029681:Arabidopsis Proteins; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D010940:Plant Proteins; D014157:Transcription Factors; D000099188:Alternative Oxidase; C523552:Myb29 protein, Arabidopsis