Enzyme-Histochemistry Technique for Visualizing the Dipeptidyl-Peptidase IV (DPP-IV) Activity in the Liver Biliary Tree.
Dipeptidyl-peptidase IV is an enzyme involved in a lot of biochemical processes, where it modifies a number of regulatory proteins by removing the terminal peptides by hydrolysis. Here we describe a histochemical method to demonstrate with accuracy and precision its in situ activity on cryostatic section of Wistar rat liver by means of a simultaneous azo-coupling method.
Department of Biology and Biotechnology "Lazzaro Spallanzani", University of Pavia, via A. Ferrata, 9, 27100, Pavia, Italy. bertone@unipv.it.
Vittorio
V
Bertone
Department of Biology and Biotechnology "Lazzaro Spallanzani", University of Pavia, via A. Ferrata, 9, 27100, Pavia, Italy.
Eleonora
E
Tarantola
Department of Biology and Biotechnology "Lazzaro Spallanzani", University of Pavia, via A. Ferrata, 9, 27100, Pavia, Italy.
Isabel
I
Freitas
D000818:Animals; D001653:Bile Ducts, Intrahepatic / Q000201:enzymology*; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism*; D004789:Enzyme Activation; D057075:Enzyme Assays / Q000379:methods; D006651:Histocytochemistry / Q000379:methods*; D008099:Liver / Q000378:metabolism; D057054:Molecular Imaging / Q000379:methods*; D051381:Rats
D018819:Dipeptidyl Peptidase 4
Cryostatic samples; Dipeptidyl-peptidase IV; Enzyme histochemistry; In situ enzyme activity