Metabolomics analysis of the effect of dissolved oxygen on spinosad production by Saccharopolyspora spinosa.
Spinosad, a universal bio-pesticide, is obtained from the soil actinomycete Saccharopolyspora spinosa. Dissolved oxygen, an important contributing factor in aerobic microbial fermentation, however, is not always available in sufficient amounts. To alleviate oxygen limitation in spinosad production, three different oxygen vectors, namely oleic acid, toluene, and n-dodecane, were added into early fermentation. Results indicated that n-dodecane was the optimal oxygen vector. Spinosad yield was increased by 44.2% compared to that in the control group in the presence of 0.5% n-dodecane, added after 120 h of incubation. Yields of the test group reached 6.52 mg/g dry cell weight (DCW), while that of the control group was limited to 4.52 mg/g DCW. Metabolomics analysis by gas chromatography coupled to mass spectrometry was performed to demonstrate the metabolism mechanism in the presence and absence of oxygen vector. In total, 78 principal intracellular metabolites in S. spinosa were detected and quantified in the presence and absence of n-dodecane. Levels of some metabolites that were related to the tricarboxylic acid cycle and pentose phosphate pathway varied significantly. Aspartic acid and glucose-1-phosphate levels varied significantly and contributed most in the distinction of the fermentation conditions and phases. The above findings give new insights into the improvement and the metabolomic characteristics of industrial spinosad production.
School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China.|Key Laboratory of System Bioengineering (Tianjin University), Ministry of Education, Tianjin, 300072, People's Republic of China.|SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, People's Republic of China.
Chunzhe
C
Lu
School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China.|Key Laboratory of System Bioengineering (Tianjin University), Ministry of Education, Tianjin, 300072, People's Republic of China.|SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, People's Republic of China.
Jing
J
Yin
School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China.|Key Laboratory of System Bioengineering (Tianjin University), Ministry of Education, Tianjin, 300072, People's Republic of China.|SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, People's Republic of China.
Fanglong
F
Zhao
School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China.|Key Laboratory of System Bioengineering (Tianjin University), Ministry of Education, Tianjin, 300072, People's Republic of China.|SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, People's Republic of China.
Feng
F
Li
School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China. wenyulu@tju.edu.cn.|Key Laboratory of System Bioengineering (Tianjin University), Ministry of Education, Tianjin, 300072, People's Republic of China. wenyulu@tju.edu.cn.|SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, People's Republic of China. wenyulu@tju.edu.cn.
Wenyu
W
Lu
D000332:Aerobiosis; D000473:Alkanes / Q000378:metabolism; D004338:Drug Combinations; D005285:Fermentation; D008401:Gas Chromatography-Mass Spectrometry; D018942:Macrolides / Q000378:metabolism*; D055432:Metabolomics; D019301:Oleic Acid / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D016930:Saccharopolyspora / Q000187:drug effects* / Q000378:metabolism*; D013997:Time Factors; D014050:Toluene / Q000378:metabolism
D000473:Alkanes; D004338:Drug Combinations; D018942:Macrolides; C007548:n-dodecane; D019301:Oleic Acid; D014050:Toluene; D010100:Oxygen; C415329:spinosad
Fermentation; Metabolic profiles; Oxygen vector; Saccharopolyspora spinosa; Spinosad