TGF-β activates genes identified by differential mRNA display in pancreatic rudiments.
The effect of TGF-β on gene activation in embryonic pancreatic rudiments was investigated using differential mRNA display. Several cDNA bands were augmented and some were suppressed in the presence of TGF-β. Differentially expressed cDNAs were re-amplified, sequenced, and sequences compared to the GeneBank database. Glucagone and brain α-tropomyosin cDNAs were identified from the group of augmented cDNAs, and B-carboxypeptidase form the group of suppressed cDNAs. PCR experiments were confirmed with Northern blots. Obtained results are in accordance with immunohistochemical findings and render differential mRNA display a useful technique in identifying differentially expressed genes in embryonic pancreatic rudiments. Several unknown differentially expressed cDNA sequences obtained in our experiments remain to be identified.
University Children's Hospital, Vrazov trg 1, SI-1000 Ljubljana, Slovenia, , , , , , SI.
Tadej
T
Battelino
INSERM U-457, Hôpital Robert Debré, 48 bd Sérurier, 75019 Paris, France, , , , , , FR.
Francisco
F
Miralles
University Children's Hospital, Vrazov trg 1, SI-1000 Ljubljana, Slovenia, , , , , , SI.
Ciril
C
Kržišnik
INSERM U-457, Hôpital Robert Debré, 48 bd Sérurier, 75019 Paris, France, , , , , , FR.
Raphaël
R
Scharfmann
INSERM U-457, Hôpital Robert Debré, 48 bd Sérurier, 75019 Paris, France, , , , , , FR.
Paul
P
Czernichow
B-carboxypeptidase; Key words: pancreatic development; TGF-β; differential mRNA display; glucagon