Genetic Methods for Studying Yeast Prions.
The recognition that certain long-known nonchromosomal genetic elements were actually prions was based not on the specific phenotypic manifestations of those elements, but rather on their unusual genetic properties. Here, we outline methods of prion assay, methods for showing the nonchromosomal inheritance, and methods for determining whether a nonchromosomal trait has the unusual characteristics diagnostic of a prion. Finally, we discuss genetic methods often useful in the study of yeast prions.
Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0830; wickner@helix.nih.gov.
Reed B
RB
Wickner
Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0830.
Herman K
HK
Edskes
Department of Pharmacology, Uniformed Services University of Health Sciences, Bethesda, Maryland 20814.
Dmitry
D
Kryndushkin
Department of Pharmacology, Uniformed Services University of Health Sciences, Bethesda, Maryland 20814.
Frank P
FP
Shewmaker
D005821:Genetic Techniques; D010641:Phenotype; D000072002:Prion Proteins / Q000235:genetics* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*
D000072002:Prion Proteins; D029701:Saccharomyces cerevisiae Proteins