Qualitative and Quantitative Analyses of Glycogen in Human Milk.
Identification as well as a detailed analysis of glycogen in human milk has not been shown yet. The present study confirmed that glycogen is contained in human milk by qualitative and quantitative analyses. High-performance anion exchange chromatography (HPAEC) and high-performance size exclusion chromatography with a multiangle laser light scattering detector (HPSEC-MALLS) were used for qualitative analysis of glycogen in human milk. Quantitative analysis was carried out by using samples obtained from the individual milks. The result revealed that the concentration of human milk glycogen varied depending on the mother's condition-such as the period postpartum and inflammation. The amounts of glycogen in human milk collected at 0 and 1-2 months postpartum were higher than in milk collected at 3-14 months postpartum. In the milk from mothers with severe mastitis, the concentration of glycogen was about 40 times higher than that in normal milk.
Institute of Health Sciences, Ezaki Glico Company, Ltd. , 4-6-5 Utajima, Nishiyodogawa-ku, Osaka 555-8502, Japan.
Hiroko
http://orcid.org/0000-0002-5231-8171
H
Matsui-Yatsuhashi
Institute of Health Sciences, Ezaki Glico Company, Ltd. , 4-6-5 Utajima, Nishiyodogawa-ku, Osaka 555-8502, Japan.
Takashi
T
Furuyashiki
Institute of Health Sciences, Ezaki Glico Company, Ltd. , 4-6-5 Utajima, Nishiyodogawa-ku, Osaka 555-8502, Japan.
Hiroki
H
Takata
Institute of Health Sciences, Ezaki Glico Company, Ltd. , 4-6-5 Utajima, Nishiyodogawa-ku, Osaka 555-8502, Japan.
Miyuki
M
Ishida
Institute of Health Sciences, Ezaki Glico Company, Ltd. , 4-6-5 Utajima, Nishiyodogawa-ku, Osaka 555-8502, Japan.
Hiroko
H
Takumi
Institute of Health Sciences, Ezaki Glico Company, Ltd. , 4-6-5 Utajima, Nishiyodogawa-ku, Osaka 555-8502, Japan.
Ryo
R
Kakutani
Institute of Health Sciences, Ezaki Glico Company, Ltd. , 4-6-5 Utajima, Nishiyodogawa-ku, Osaka 555-8502, Japan.
Hiroshi
H
Kamasaka
Nagao Maternity Hospital , Terado-cho, Muko-shi, Kyoto 617-0002, Japan.
Saeko
S
Nagao
Department of Food Science and Nutrition, School of Human Cultures, University of Shiga Prefecture , 2500 Hassaka-cho, Hikone-shi, Shiga 522-8533, Japan.
Junko
J
Hirose
Institute of Health Sciences, Ezaki Glico Company, Ltd. , 4-6-5 Utajima, Nishiyodogawa-ku, Osaka 555-8502, Japan.
Takashi
T
Kuriki
D000328:Adult; D005260:Female; D006003:Glycogen / Q000032:analysis*; D006801:Humans; D013058:Mass Spectrometry; D008895:Milk, Human / Q000737:chemistry*
D006003:Glycogen
enzymatic treatment; glycogen; human milk; inflammation; period postpartum