Anti-Inflammatory Activity of Sanghuangporus sanghuang Mycelium.
Acute lung injury (ALI) is characterized by inflammation of the lung tissue and oxidative injury caused by excessive accumulation of reactive oxygen species. Studies have suggested that anti-inflammatory or antioxidant agents could be used for the treatment of ALI with a good outcome. Therefore, our study aimed to test whether the mycelium extract ofSanghuangporus sanghuang(SS-1), believed to exhibit antioxidant and anti-inflammatory properties, could be used against the excessive inflammatory response associated with lipopolysaccharides (LPS)-induced ALI in mice and to investigate its possible mechanism of action. The experimental results showed that the administration of SS-1 could inhibit LPS-induced inflammation. SS-1 could reduce the number of inflammatory cells, inhibit myeloperoxidase (MPO) activity, regulate the TLR4/PI3K/Akt/mTOR pathway and the signal transduction of NF-κB and MAPK pathways in the lung tissue, and inhibit high mobility group box-1 protein 1 (HNGB1) activity in BALF. In addition, SS-1 could affect the synthesis of antioxidant enzymes Heme oxygenase 1 (HO-1) and Thioredoxin-1 (Trx-1) in the lung tissue and regulate signal transduction in the KRAB-associated protein-1 (KAP1)/nuclear factor erythroid-2-related factor Nrf2/Kelch Like ECH associated Protein 1 (Keap1) pathway. Histological results showed that administration of SS-1 prior to induction could inhibit the large-scale LPS-induced neutrophil infiltration of the lung tissue. Therefore, based on all experimental results, we propose that SS-1 exhibits a protective effect against LPS-induced ALI in mice. The mycelium ofS. sanghuangcan potentially be used for the treatment or prevention of inflammation-related diseases.
School of Pharmacy, China Medical University, Taichung 404, Taiwan. linwc0913@gmail.com.
Wang-Ching
WC
Lin
Department of Health and Nutrition Biotechnology, Asia University, Taichung 413, Taiwan. dengjs@asia.edu.tw.
Jeng-Shyan
JS
Deng
School of Pharmacy, China Medical University, Taichung 404, Taiwan. sshuang@mail.cmu.edu.tw.
Shyh-Shyun
SS
Huang
Department of Biology, National Museum of Natural Science, Taichung 404, Taiwan. shwu@mail.nmns.edu.tw.
Sheng-Hua
SH
Wu
Grape King Biotechnology Center, Chung-Li City 320, Taiwan. gkbioeng@grapeking.com.tw.
Chin-Chu
CC
Chen
Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung 404, Taiwan. linwr0627@gmail.com.
Wan-Rong
WR
Lin
School of Pharmacy, China Medical University, Taichung 404, Taiwan. hylin@mail.cmu.edu.tw.
Hui-Yi
HY
Lin
Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung 404, Taiwan. gjhuang@mail.cmu.edu.tw.
Guan-Jhong
GJ
Huang
D055371:Acute Lung Injury / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism* / Q000473:pathology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D001487:Basidiomycota / Q000737:chemistry*; D001688:Biological Products / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D051547:Heme Oxygenase-1 / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism; D008070:Lipopolysaccharides / Q000009:adverse effects; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D025282:Mycelium / Q000737:chemistry*; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt; D015398:Signal Transduction / Q000187:drug effects; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism; D013879:Thioredoxins / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D001688:Biological Products; D016207:Cytokines; D051547:Heme Oxygenase-1; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D020928:Mitogen-Activated Protein Kinases; D051267:NF-E2-Related Factor 2; D016328:NF-kappa B; D009569:Nitric Oxide; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases; D013879:Thioredoxins; D051197:Toll-Like Receptor 4
HNGB; HO-1; KAP1/Nrf2 pathway; PI3K/Akt/mTOR pathways; acute lung injury; lipopolysaccharide; mycelium of Sanghuangporus sanghuang