Physiological Responses and Gene Co-Expression Network of Mycorrhizal Roots under K+Deprivation.
Arbuscular mycorrhizal (AM) associations enhance the phosphorous and nitrogen nutrition of host plants, but little is known about their role in potassium (K+) nutrition.Medicago truncatulaplants were cocultured with the AM fungusRhizophagus irregularisunder high and low K+regimes for 6 weeks. We determined how K+deprivation affects plant development and mineral acquisition and how these negative effects are tempered by the AM colonization. The transcriptional response of AM roots under K+deficiency was analyzed by whole-genome RNA sequencing. K+deprivation decreased root biomass and external K+uptake and modulated oxidative stress gene expression inM. truncatularoots. AM colonization induced specific transcriptional responses to K+deprivation that seem to temper these negative effects. A gene network analysis revealed putative key regulators of these responses. This study confirmed that AM associations provide some tolerance to K+deprivation to host plants, revealed that AM symbiosis modulates the expression of specific root genes to cope with this nutrient stress, and identified putative regulators participating in these tolerance mechanisms.
Department of Bacteriology (K.G., J.-M.A.), Department of Computer Sciences (S.R.), and Department of Agronomy (J.-M.A.), University of Wisconsin, Madison, Wisconsin 53706.|Wisconsin Institute for Discovery, University of Wisconsin, Madison, Wisconsin 53715 (D.C., S.R.); and.|Department of Biostatistics and Medical Informatics, University of Wisconsin, Madison, Wisconsin 53792 (S.R.).
Kevin
0000-0003-0821-1024
K
Garcia
Department of Bacteriology (K.G., J.-M.A.), Department of Computer Sciences (S.R.), and Department of Agronomy (J.-M.A.), University of Wisconsin, Madison, Wisconsin 53706.|Wisconsin Institute for Discovery, University of Wisconsin, Madison, Wisconsin 53715 (D.C., S.R.); and.|Department of Biostatistics and Medical Informatics, University of Wisconsin, Madison, Wisconsin 53792 (S.R.).
Deborah
0000-0002-7324-0131
D
Chasman
Department of Bacteriology (K.G., J.-M.A.), Department of Computer Sciences (S.R.), and Department of Agronomy (J.-M.A.), University of Wisconsin, Madison, Wisconsin 53706.|Wisconsin Institute for Discovery, University of Wisconsin, Madison, Wisconsin 53715 (D.C., S.R.); and.|Department of Biostatistics and Medical Informatics, University of Wisconsin, Madison, Wisconsin 53792 (S.R.).
Sushmita
S
Roy
Department of Bacteriology (K.G., J.-M.A.), Department of Computer Sciences (S.R.), and Department of Agronomy (J.-M.A.), University of Wisconsin, Madison, Wisconsin 53706; jeanmichel.ane@wisc.edu.|Wisconsin Institute for Discovery, University of Wisconsin, Madison, Wisconsin 53715 (D.C., S.R.); and jeanmichel.ane@wisc.edu.|Department of Biostatistics and Medical Informatics, University of Wisconsin, Madison, Wisconsin 53792 (S.R.) jeanmichel.ane@wisc.edu.
Jean-Michel
0000-0002-3128-9439
JM
Ané
D016000:Cluster Analysis; D020869:Gene Expression Profiling / Q000379:methods; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks; D054884:Host-Pathogen Interactions; D046913:Medicago truncatula / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D038821:Mycorrhizae / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D011188:Potassium / Q000378:metabolism*; D013559:Symbiosis / Q000502:physiology; D013997:Time Factors
D010940:Plant Proteins; D011188:Potassium