A novel in situ silver/hyaluronan bio-nanocomposite fabrics for wound and chronic ulcer dressing: In vitro and in vivo evaluations.
In-situ formed hyaluronan/silver (HA/Ag) nanoparticles (NPs) were used to prepare composite fibers/fabrics for the first time. Different concentrations of silver nitrate (1, 2mg/100ml) were added at ambient temperature to sodium hyaluronate solution (40mg/ml), then the pH was increased to 8 by adding sodium hydroxide. The in-situ formed HA/Ag-NPs were used to prepare fibers/nonwoven fabrics by wet-dry-spinning technique (WDST). UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively. The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25±2, nm size (TEM, DLS) and acceptable mechanical properties. The XRD analysis showed that the in-situ preparation of Ag-NPs increased the crystallinity of the resultant fabrics as well as the thermal stability. The antibacterial performance of medical HA/Ag-NPs fabrics was evaluated against gram negative bacteria E. coli K12, exhibiting significant bactericidal activity. The fibers did not show any cytotoxicity against human keratinocyte cell line (HaCaT). In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process. Based on our results, the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering.
CEITECCentral European Institute of Technology, Brno University of Technology, Brno, Czechia; SCITEG, a.s., Brno, Czechia; Pretreatment and Finishing of Cellulosic Fibers, Textile Research Division, National Research Centre, Dokki, Cairo, Egypt. Electronic address: abdel-mohsen@ceitec.vutbr.cz.
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AM
Abdel-Mohsen
CEITECCentral European Institute of Technology, Brno University of Technology, Brno, Czechia; SCITEG, a.s., Brno, Czechia; Faculty of Chemistry, Institute of Materials Chemistry, Brno University of Technology, Brno, Czechia.
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J
Jancar
CEITECCentral European Institute of Technology, Brno University of Technology, Brno, Czechia.
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RM
Abdel-Rahman
Masaryk University, Faculty of Science, Department of Experimental Biology, Brno, Czechia.
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L
Vojtek
Masaryk University, Faculty of Science, Department of Experimental Biology, Brno, Czechia.
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P
Hyršl
Masaryk University, Faculty of Science, Department of Experimental Biology, Brno, Czechia.
M
M
Dušková
Masaryk University, Faculty of Science, Department of Experimental Biology, Brno, Czechia.
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H
Nejezchlebová
D000818:Animals; D001458:Bandages; D002478:Cells, Cultured; D003460:Crystallization; D004355:Drug Stability; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry / Q000627:therapeutic use*; D008297:Male; D008826:Microbial Sensitivity Tests; D057139:Nanofibers / Q000737:chemistry* / Q000627:therapeutic use* / Q000648:ultrastructure; D010316:Particle Size; D051381:Rats; D012834:Silver / Q000737:chemistry*; D014456:Ulcer / Q000188:drug therapy*; D014945:Wound Healing / Q000187:drug effects*
D012834:Silver; D006820:Hyaluronic Acid
Cytotoxicity; Hyaluronan; In-situ silver; Wound dressing