A new pyrazoline-based probe of quenched fluorescent reversible recognition for Cu2+and its application in cells.
A new pyrazoline-based probe D was designed and synthesized, which can be used as a highly sensitive, selective and reversible recognizing fluorescent to detect Cu2+. The recognition properties of this compound was investigated by UV-vis absorption and fluorescence spectrophotometry. The results showed that the probe D forms a 1:1 complex with Cu2+and displayed a linear fluorescence response to Cu2+with a detection limit of 1.94×10-7M. In addition, the probe have a good biocompatibility in living cells.
School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou 730050, China. Electronic address: yingpengzhang@126.com.
Ying-Peng
YP
Zhang
School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou 730050, China.
Yu-Ying
YY
Dong
School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou 730050, China. Electronic address: yangguang@lut.cn.
Yun-Shang
YS
Yang
Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Hui-Chen
HC
Guo
School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou 730050, China.
Bi-Xia
BX
Cao
Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Shi-Qi
SQ
Sun
D000818:Animals; D016923:Cell Death; D002460:Cell Line; D003300:Copper / Q000032:analysis*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D011720:Pyrazoles / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D005456:Fluorescent Dyes; D011720:Pyrazoles; D003300:Copper
Cu(2+) recognition fluorescence probe living cells