Solvent-Responsive Molecularly Imprinted Nanogels for Targeted Protein Analysis in MALDI-TOF Mass Spectrometry.
Molecular imprinted poly(acrylamido)-derivative nanogels have shown their selectivity to bind the protein human serum transferrin (HTR) and also showed their capability for instantaneous solvent-induced modification upon the addition of acetonitrile. Integrated to matrix-assisted laser desorption/ionization time-of-flight mass analysis the HTR-imprinted solvent-responsive nanogels permitted the determination of HTR straight from serum and offered novel perspectives in targeted protein analysis.
Department of Physics, University of Trento , Via Sommarive 14, 38123 Trento, Italy.
Maddalena
M
Bertolla
Department of Biotechnology, University of Verona , Strada Le Grazie 15, 37134 Verona, Italy.
Lucia
L
Cenci
Department of Physics, University of Trento , Via Sommarive 14, 38123 Trento, Italy.
Andrea
A
Anesi
Department of Molecular Sciences and Nanosystems, University Cà Foscari Venezia , Via Torino 155/b, 30173 Venice, Italy.
Emmanuele
E
Ambrosi
Department of Diagnostics and Public Health, Unit of Forensic Medicine, University of Verona , P.le L.A. Scuro 10, 37134 Verona, Italy.
Franco
F
Tagliaro
Fondazione Bruno Kessler CMM-MNF , Via Sommarive 18, 38123 Trento, Italy.
Lia
L
Vanzetti
Department of Physics, University of Trento , Via Sommarive 14, 38123 Trento, Italy.
Graziano
G
Guella
Department of Biotechnology, University of Verona , Strada Le Grazie 15, 37134 Verona, Italy.
Alessandra Maria
http://orcid.org/0000-0002-2542-8412
AM
Bossi
D006801:Humans; D054802:Molecular Imprinting; D053758:Nanoparticles; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D012997:Solvents; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D012997:Solvents; D011092:Polyethylene Glycols; D011094:Polyethyleneimine
MALDI; mass spectrometry; molecularly imprinted polymers; nanogels; polyacrylamide; responsive material; serum transferrin; targeted protein analysis