Thermal acclimation and subspecies-specific effects on heart and brain mitochondrial performance in a eurythermal teleost (Fundulus heteroclitus).
Mitochondrial performance may play a role in setting whole-animal thermal tolerance limits and their plasticity, but the relative roles of adjustments in mitochondrial performance across different highly aerobic tissues remain poorly understood. We compared heart and brain mitochondrial responses to acute thermal challenges and to thermal acclimation using high-resolution respirometry in two locally adapted subspecies of Atlantic killifish (Fundulus heteroclitus). We predicted that 5°C acclimation would result in compensatory increases in mitochondrial performance, while 33°C acclimation would cause suppression of mitochondrial function to minimize the effects of high temperature on mitochondrial metabolism. In contrast, acclimation to both 33 and 5°C decreased mitochondrial performance compared with fish acclimated to 15°C. These adjustments could represent an energetic cost-saving mechanism at temperature extremes. Acclimation responses were similar in both heart and brain; however, this effect was smaller in the heart, which might indicate its importance in maintaining whole-animal thermal performance. Alternatively, larger acclimation effects in the brain might indicate greater thermal sensitivity compared with the heart. We detected only modest differences between subspecies that were dependent on the tissue assayed. These data demonstrate extensive plasticity in mitochondrial performance following thermal acclimation in killifish, and indicate that the extent of these responses differs between tissues, highlighting the importance and complexity of mitochondrial regulation in thermal acclimation in eurytherms.
Department of Zoology, University of British Columbia, 6270 University Blvd, Vancouver, British Columbia, Canada, V6T 1Z4 dchungch@zoology.ubc.ca.
Dillon James
0000-0002-0329-3828
DJ
Chung
Department of Zoology, University of British Columbia, 6270 University Blvd, Vancouver, British Columbia, Canada, V6T 1Z4.
Heather J
HJ
Bryant
Department of Zoology, University of British Columbia, 6270 University Blvd, Vancouver, British Columbia, Canada, V6T 1Z4.
Patricia M
PM
Schulte
D000064:Acclimatization; D000818:Animals; D001921:Brain / Q000502:physiology; D002950:Citrate (si)-Synthase / Q000378:metabolism; D029941:Fish Proteins / Q000378:metabolism; D023781:Fundulidae / Q000502:physiology*; D006321:Heart / Q000502:physiology; D008928:Mitochondria / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D013045:Species Specificity; D013696:Temperature
D029941:Fish Proteins; D002950:Citrate (si)-Synthase
Fish; Killifish; Local adaptation; Oxidative phosphorylation; Temperature; Thermal performance