Characterization of a mycobacterial cellulase and its impact on biofilm- and drug-induced cellulose production.
It was recently shown that Mycobacterium tuberculosis produces cellulose which forms an integral part of its extracellular polymeric substances within a biofilm set-up. Using Mycobacterium smegmatis as a proxy model organism, we demonstrate that M. smegmatis biofilms treated with purified MSMEG_6752 releases the main cellulose degradation-product (cellobiose), detected by using ionic chromatography, suggesting that MSMEG_6752 encodes a cellulase. Its overexpression in M. smegmatis prevents spontaneous biofilm formation. Moreover, the method reported here allowed detecting cellobiose when M. smegmatis cultures were exposed to a subinhibitory dose of rifampicin. Overall, this study highlights the role of the MSMEG_6752 in managing cellulose production induced during biofilm formation and antibiotic stress response.
Université de Montpellier, CNRS, Centre d'étude d'agents Pathogènes et Biotechnologies pour la Santé (CPBS), FRE3689, 34293 Montpellier, France.
Niël
N
Van Wyk
INRA, Biodiversité et Biotechnologie Fongiques, UMR 1163, Université Aix-Marseille, 163 Avenue de Luminy, 13288 Marseille, France.
David
D
Navarro
Université de Montpellier, CNRS, Centre d'étude d'agents Pathogènes et Biotechnologies pour la Santé (CPBS), FRE3689, 34293 Montpellier, France.
Mickaël
M
Blaise
INRA, Biodiversité et Biotechnologie Fongiques, UMR 1163, Université Aix-Marseille, 163 Avenue de Luminy, 13288 Marseille, France.
Jean-Guy
JG
Berrin
Architecture et Fonction des Macromolécules Biologiques, Centre National de la Recherche Scientifique (CNRS), Université Aix-Marseille, 163 Avenue de Luminy, 13288 Marseille, France.|INRA, USC 1408 AFMB, 13288 Marseille, France.|Department of Biological Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
Bernard
B
Henrissat
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement, Faculté de Médecine, Aix-Marseille Université, 27 Bd Jean Moulin, 13005 Marseille, France.
Michel
M
Drancourt
Université de Montpellier, CNRS, Centre d'étude d'agents Pathogènes et Biotechnologies pour la Santé (CPBS), FRE3689, 34293 Montpellier, France.|INSERM, CPBS, 1919 route de Mende, 34293 Montpellier, France.
Laurent
L
Kremer
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D002480:Cellulase / Q000737:chemistry* / Q000378:metabolism; D002482:Cellulose / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D002845:Chromatography; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D020102:Mycobacterium smegmatis / Q000201:enzymology*; D009169:Mycobacterium tuberculosis / Q000201:enzymology; D012293:Rifampin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D002482:Cellulose; D002480:Cellulase; D012293:Rifampin
Mycobacterium; biofilm; cellulase; cellulose; rifampicin