Cutting Edge:Helicobacter pyloriInduces Nuclear Hypersegmentation and Subtype Differentiation of Human Neutrophils In Vitro.
Helicobacter pyloriinfects the human stomach and causes a spectrum of disease that includes gastritis, peptic ulcers, and gastric adenocarcinoma. A chronic, neutrophil-rich inflammatory response characterizes this infection. It is established thatH. pyloristimulates neutrophil chemotaxis and a robust respiratory burst, but other aspects of this interaction are incompletely defined. We demonstrate here thatH. pyloriinduces N1-like subtype differentiation of human neutrophils as indicated by profound nuclear hypersegmentation, a CD62Ldim, CD16bright, CD11bbright, CD66bbright, CD63brightsurface phenotype, proinflammatory cytokine secretion, and cytotoxicity. Hypersegmentation requires direct neutrophil-H. pyloricontact as well as transcription and both host and bacterial protein synthesis, but not urease, NapA, VacA, CagA, or CagT. The concept of neutrophil plasticity is new and, to our knowledge, these data are the first evidence that neutrophils can undergo subtype differentiation in vitro in response to bacterial pathogen infection. We hypothesize that these changes favorH. pyloripersistence and disease.
Inflammation Program, University of Iowa, Iowa City, IA 52242.|Department of Internal Medicine, University of Iowa, Iowa City, IA 52242.
Laura C
LC
Whitmore
Inflammation Program, University of Iowa, Iowa City, IA 52242.|Department of Internal Medicine, University of Iowa, Iowa City, IA 52242.
Megan N
MN
Weems
Inflammation Program, University of Iowa, Iowa City, IA 52242; lee-ann-allen@uiowa.edu.|Department of Internal Medicine, University of Iowa, Iowa City, IA 52242.|Department of Microbiology, University of Iowa, Iowa City, IA 52242; and.|Iowa City VA Health Care System, Iowa City, IA 52246.
Lee-Ann H
LH
Allen
D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000648:ultrastructure; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D016480:Helicobacter pylori / Q000502:physiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D007960:Leukocyte Disorders; D009504:Neutrophils / Q000276:immunology / Q000382:microbiology* / Q000502:physiology* / Q000648:ultrastructure; D010641:Phenotype; D014510:Urease / Q000235:genetics
D015703:Antigens, CD; D001426:Bacterial Proteins; D016207:Cytokines; D014510:Urease