Robust, universal biomarker assay to detect senescent cells in biological specimens.
Cellular senescence contributes to organismal development, aging, and diverse pathologies, yet available assays to detect senescent cells remain unsatisfactory. Here, we designed and synthesized a lipophilic, biotin-linked Sudan Black B (SBB) analogue suitable for sensitive and specific, antibody-enhanced detection of lipofuscin-containing senescent cells in any biological material. This new hybrid histo-/immunochemical method is easy to perform, reliable, and universally applicable to assess senescence in biomedicine, from cancer research to gerontology.
Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
Konstantinos
K
Evangelou
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece.
Nikolaos
N
Lougiakis
Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
Sophia V
SV
Rizou
Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
Athanassios
A
Kotsinas
Laboratory of Cell Proliferation and Ageing, Institute of Biosciences and Applications, National Centre for Scientific Research 'Demokritos', Athens, Greece.
Dimitris
D
Kletsas
Tumor Suppression Group, Molecular Oncology Program, Spanish National Cancer Research Centre (CNIO), Madrid, Spain.
Daniel
D
Muñoz-Espín
Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
Nikolaos G
NG
Kastrinakis
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece.
Nicole
N
Pouli
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece.
Panagiotis
P
Marakos
Molecular and Clinical Cancer Sciences, Manchester Cancer Research Centre, Manchester Academic Health Sciences Centre, University of Manchester, Manchester, UK.
Paul
P
Townsend
Tumor Suppression Group, Molecular Oncology Program, Spanish National Cancer Research Centre (CNIO), Madrid, Spain.
Manuel
M
Serrano
Danish Cancer Society Research Center, Copenhagen, Denmark.|Science For Life Laboratory, Division of Translational Medicine and Chemical Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.
Jiri
J
Bartek
Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, Athens, Greece.|Molecular and Clinical Cancer Sciences, Manchester Cancer Research Centre, Manchester Academic Health Sciences Centre, University of Manchester, Manchester, UK.|Biomedical Research Foundation, Academy of Athens, Athens, Greece.
Vassilis G
VG
Gorgoulis
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D015415:Biomarkers / Q000032:analysis*; D001710:Biotin / Q000378:metabolism; D045744:Cell Line, Tumor; D016922:Cellular Senescence; D006801:Humans; D051379:Mice
D015415:Biomarkers; D001710:Biotin
Sudan Black B; aging; biotin‐linked compounds; immunohistochemistry; senescence