Chemical composition differentiation of Shen-Shuai-Ning granule between combined decoction and separated decoction using HPLC-DAD-ESI-QTOF-MS.
Shen-Shuai-Ning (SSN) granule, a traditional Chinese medicine formula, is widely used in clinical practice for treating chronic renal failure. However, its detailed chemical profile is unknown. Here, HPLC-ESI-QTOF-MS was employed for the systematic chemical analysis of SSN. A total of 52 compounds were identified and the characteristic ions of the compounds were described. Furthermore, chemical consistency between the combined decoction and the separated decoction of SSN was evaluated using HPLC-DAD. A chemical comparison between two preparations of SSN granule (combined decoction and separated decoction of Coptides Rhizoma) indicated a significant difference in the content of many compounds, including salvianolic acid A, salvianolic acid B, berberine, palmatine and epiberberine. As a result, separated decoction of Coptides Rhizoma would lead to a significantly decrease in depsides in Salviae Miltiorrhizae Radix et Rhizoma and an increase in alkaloids in Coptidis Rhizoma.
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Jianli
J
Liu
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Yu
Y
Zhang
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Yimeng
Y
Hao
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Ying
Y
Zhao
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Yanjiao
Y
Li
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Rulan
R
Qin
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Chongning
C
Lv
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Jincai
http://orcid.org/0000-0002-7331-1251
J
Lu
D001572:Benzofurans; D001600:Berberine Alkaloids; D002109:Caffeic Acids; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004339:Drug Compounding; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry*; D047309:Flavones; D007773:Lactates; D012503:Saponins; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053630:Depsides
D001572:Benzofurans; D001600:Berberine Alkaloids; D002109:Caffeic Acids; D004365:Drugs, Chinese Herbal; D047309:Flavones; D007773:Lactates; D012503:Saponins; C532547:Shenshuaining; C066201:salvianolic acid A; C076944:salvianolic acid B; D053630:Depsides
HPLC-Dad-ESI-QTOF/MS; Shen Shuai Ning; chemical composition identification; combined decoction and separated decoction