Biochemical and genetic functional dissection of the P38 viral suppressor of RNA silencing.
Phytoviruses encode viral suppressors of RNA silencing (VSRs) to counteract the plant antiviral silencing response, which relies on virus-derived small interfering (si)RNAs processed by Dicer RNaseIII enzymes and subsequently loaded into ARGONAUTE (AGO) effector proteins. Here, a tobacco cell-free system was engineered to recapitulate the key steps of antiviral RNA silencing and, in particular, the most upstream double-stranded (ds)RNA processing reaction, not kinetically investigated thus far in the context of plant VSR studies. Comparative biochemical analyses of distinct VSRs in the reconstituted assay showed that in all cases tested, VSR interactions with siRNA duplexes inhibited the loading, but not the activity, of antiviral AGO1 and AGO2. Turnip crinkle virus P38 displayed the additional and unique property to bind both synthetic and RNA-dependent-RNA-polymerase-generated long dsRNAs, and inhibited the processing into siRNAs. Single amino acid substitutions in P38 could dissociate dsRNA-processing from AGO-loading inhibition in vitro and in vivo, illustrating dual-inhibitory strategies discriminatively deployed within a single viral protein, which, we further show, are bona fide suppressor functions that evolved independently of the conserved coat protein function of P38.
Department of Biology, Swiss Federal Institute of Technology (ETH), 8092 Zürich, Switzerland.
Taichiro
T
Iki
Department of Biology, Swiss Federal Institute of Technology (ETH), 8092 Zürich, Switzerland.
Marie-Aude
MA
Tschopp
Department of Biology, Swiss Federal Institute of Technology (ETH), 8092 Zürich, Switzerland.
Olivier
O
Voinnet
D060565:Argonaute Proteins / Q000378:metabolism; D019184:Carmovirus; D010940:Plant Proteins / Q000378:metabolism; D017354:Point Mutation; D034622:RNA Interference; D012323:RNA Processing, Post-Transcriptional; D012330:RNA, Double-Stranded / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D034743:RNA-Induced Silencing Complex / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D060565:Argonaute Proteins; D010940:Plant Proteins; D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D034743:RNA-Induced Silencing Complex; D014764:Viral Proteins
ARGONAUTE (AGO); Dicer-like (DCL); Nicotiana tabacum BY-2 cell lysate (BYL); P38; Turnip crinkle virus (TCV); viral suppressor of RNA silencing (VSR)