Enhanced antitumor efficacy of resveratrol-loaded nanocapsules in colon cancer cells: physicochemical and biological characterization.
The aim of present work was to prepare resveratrol-loaded lipid-core-nanocapsule (RSV-LNC) and to characterize its ability to target the colon cancer cells.



The lipid-core nanocapsule was prepared by precipitation method. The nanoparticle was prepared and evaluated regarding physical, chemical and biological parameters.



The average size of optimized nanocapsule was ~159 nm with a uniform size distribution index of 0.15 (PDI). The RSV-LNC showed a controlled and sustained release pattern with maximum release up to ~70% by the end of 48h study period. LNC showed an excellent cellular uptake potential. LNC showed a typical endocytosis-mediated cellular internalization process and located in the cell cytoplasm.



Importantly, RSV encapsulated in a nanocapsule showed a superior anticancer effect in HT29 cancer cells than compared to that of free RSV. Consistently, RSV-LNC showed a remarkable ~36% of cell apoptosis indicating its superior anticancer effect.



Based on the in vitro studies, RSV encapsulated in a nanocapsule showed promising potential to increase the therapeutic efficacy in colon cancer cells; however, further studies on animal models are warranted to confirm the improved effects of RSV nanoformulations.
Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, P.R. China. xiongjianping36@hotmail.com.
M
M
Feng
L-X
LX
Zhong
Z-Y
ZY
Zhan
Z-H
ZH
Huang
J-P
JP
Xiong
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000473:pathology*; D004337:Drug Carriers / Q000737:chemistry*; D019073:HT29 Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry; D053769:Nanocapsules / Q000737:chemistry; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
D000970:Antineoplastic Agents; D004337:Drug Carriers; D008055:Lipids; D053769:Nanocapsules; D013267:Stilbenes; D000077185:Resveratrol