Mitochondrial genome diversity in dagger and needle nematodes (Nematoda: Longidoridae).
Dagger and needle nematodes included in the family Longidoridae (viz. Longidorus, Paralongidorus, and Xiphinema) are highly polyphagous plant-parasitic nematodes in wild and cultivated plants and some of them are plant-virus vectors (nepovirus). The mitochondrial (mt) genomes of the dagger and needle nematodes, Xiphinema rivesi, Xiphinema pachtaicum, Longidorus vineacola and Paralongidorus litoralis were sequenced in this study. The four circular mt genomes have an estimated size of 12.6, 12.5, 13.5 and 12.7 kb, respectively. Up to date, the mt genome of X. pachtaicum is the smallest genome found in Nematoda. The four mt genomes contain 12 protein-coding genes (viz. cox1-3, nad1-6, nad4L, atp6 and cob) and two ribosomal RNA genes (rrnL and rrnS), but the atp8 gene was not detected. These mt genomes showed a gene arrangement very different within the Longidoridae species sequenced, with the exception of very closely related species (X. americanum and X. rivesi). The sizes of non-coding regions in the Longidoridae nematodes were very small and were present in a few places in the mt genome. Phylogenetic analysis of all coding genes showed a closer relationship between Longidorus and Paralongidorus and different phylogenetic possibilities for the three Xiphinema species.
Instituto de Agricultura Sostenible (IAS), Agencia Estatal Consejo Superior de Investigaciones Científicas (CSIC), Avda. Menéndez Pidal s/n, 14004 Córdoba, Spain.
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Palomares-Rius
Instituto de Agricultura Sostenible (IAS), Agencia Estatal Consejo Superior de Investigaciones Científicas (CSIC), Avda. Menéndez Pidal s/n, 14004 Córdoba, Spain.
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Cantalapiedra-Navarrete
Instituto de Agricultura Sostenible (IAS), Agencia Estatal Consejo Superior de Investigaciones Científicas (CSIC), Avda. Menéndez Pidal s/n, 14004 Córdoba, Spain.
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Archidona-Yuste
Cell and Molecular Sciences, The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, United Kingdom.
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Blok
Instituto de Agricultura Sostenible (IAS), Agencia Estatal Consejo Superior de Investigaciones Científicas (CSIC), Avda. Menéndez Pidal s/n, 14004 Córdoba, Spain.
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Castillo
D000818:Animals; D001482:Base Composition; D003062:Codon; D018509:DNA, Helminth; D050259:Genes, Mitochondrial; D014644:Genetic Variation; D054629:Genome, Mitochondrial; D023281:Genomics / Q000379:methods; D009348:Nematoda / Q000145:classification* / Q000235:genetics*; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D010802:Phylogeny
D003062:Codon; D018509:DNA, Helminth