Two White Spot Syndrome Virus MicroRNAs Target theDorsalGene To Promote Virus Infection in Marsupenaeus japonicus Shrimp.
In eukaryotes, microRNAs (miRNAs) serve as regulators of many biological processes, including virus infection. An miRNA can generally target diverse genes during virus-host interactions. However, the regulation of gene expression by multiple miRNAs has not yet been extensively explored during virus infection. This study found that the Spaztle (Spz)-Toll-Dorsal-antilipopolysaccharide factor (ALF) signaling pathway plays a very important role in antiviral immunity against invasion of white spot syndrome virus (WSSV) in shrimp (Marsupenaeus japonicus).Dorsal, the central gene in the Toll pathway, was targeted by two viral miRNAs (WSSV-miR-N13 and WSSV-miR-N23) during WSSV infection. The regulation ofDorsalexpression by viral miRNAs suppressed the Spz-Toll-Dorsal-ALF signaling pathway in shrimpin vivo, leading to virus infection. Our study contributes novel insights into the viral miRNA-mediated Toll signaling pathway during the virus-host interaction.IMPORTANCEAn miRNA can target diverse genes during virus-host interactions. However, the regulation of gene expression by multiple miRNAs during virus infection has not yet been extensively explored. The results of this study indicated that the shrimpDorsalgene, the central gene in the Toll pathway, was targeted by two viral miRNAs during infection with white spot syndrome virus. Regulation ofDorsalexpression by viral miRNAs suppressed the Spz-Toll-Dorsal-ALF signaling pathway in shrimpin vivo, leading to virus infection. Our study provides new insight into the viral miRNA-mediated Toll signaling pathway in virus-host interactions.
Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory for Marine Science and Technology and College of Life Sciences, Zhejiang University, Hangzhou, People's Republic of China.|Jiangsu Key Laboratory for Biodiversity & Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, Nanjing, China.
Qian
Q
Ren
Jiangsu Key Laboratory for Biodiversity & Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, Nanjing, China.
Xin
X
Huang
Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory for Marine Science and Technology and College of Life Sciences, Zhejiang University, Hangzhou, People's Republic of China.
Yalei
Y
Cui
Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong, China.
Jiejie
J
Sun
Jiangsu Key Laboratory for Biodiversity & Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, Nanjing, China.
Wen
W
Wang
Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory for Marine Science and Technology and College of Life Sciences, Zhejiang University, Hangzhou, People's Republic of China zxb0812@zju.edu.cn.
Xiaobo
X
Zhang
D000818:Animals; D054884:Host-Pathogen Interactions; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D033561:Penaeidae / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000037:antagonists & inhibitors*; D014779:Virus Replication; D046848:White spot syndrome virus 1 / Q000235:genetics / Q000276:immunology / Q000502:physiology*
D035683:MicroRNAs; D012367:RNA, Viral; D014157:Transcription Factors
Dorsal; Toll signaling pathway; microRNAs; shrimp; virus