Alcohol Dehydrogenases and Acetaldehyde Dehydrogenases are Beneficial for Decidual Stromal Cells to Resist the Damage from Alcohol.
The aim of this study was to examine the effect of alcohol on the decidualization of human endometrial stromal cells during early pregnancy.



During in vitro decidualization, human endometrial stromal cells were treated with alcohol, 4-methylpyrazole hydrochloride (FPZ), the inhibitor of alcohol dehydrogenases (ADHs), and tetraethylthiuram disulfide (DSF), the inhibitor of acetaldehyde dehydrogenases (ALDHs), respectively. Cell viability and decidualization were examined. Apoptosis and proliferation were also evaluated.



The findings showed that ADHs and ALDHs were up-regulated during decidualization. After alcohol treatment, the cell viability of decidual stromal cells was significantly higher than control, which was abrogated by FPZ or DSF. When cells were treated with alcohol, proliferation-related signal pathways were up-regulated in decidualized cells. Additionally, FOXO1 transcriptionally up-regulates ADH1B.



Our study provided an evidence that highly expressed ADHs and ALDHs endow decidual stromal cells an ability to alleviate the harm from alcohol.
Department of Biology, Shantou University, 243 Daxue Rd., Shantou 515063, China.|College of Veterinary Medicine, South China Agricultural University, 483 Wushan Rd., Guangzhou 510642, China.
Zi-Long
ZL
Li
College of Veterinary Medicine, South China Agricultural University, 483 Wushan Rd., Guangzhou 510642, China.
Meng-Qi
MQ
Li
College of Veterinary Medicine, South China Agricultural University, 483 Wushan Rd., Guangzhou 510642, China.
Shu-Yun
SY
Li
College of Veterinary Medicine, South China Agricultural University, 483 Wushan Rd., Guangzhou 510642, China.
Yong-Sheng
YS
Fu
College of Veterinary Medicine, South China Agricultural University, 483 Wushan Rd., Guangzhou 510642, China.
Zeng-Ming
ZM
Yang
D000426:Alcohol Dehydrogenase / Q000096:biosynthesis*; D000445:Aldehyde Oxidoreductases / Q000096:biosynthesis*; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D003656:Decidua / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology*; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D006801:Humans; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology
D000431:Ethanol; D000426:Alcohol Dehydrogenase; D000445:Aldehyde Oxidoreductases; C018699:aldehyde dehydrogenase (NAD(P)+)