The miR-599 promotes non-small cell lung cancer cell invasion via SATB2.
MicroRNAs (miRNAs) play important roles in the pathogenesis of many types of cancers by negatively regulating gene expression at posttranscriptional level. Here, we identified that miR-599 is up-regulated in non-small cell lung cancer (NSCLC) patients. It promoted NSCLC cell proliferation by negatively regulating SATB2. In NSCLC cell lines, CCK-8 proliferation assay indicated that the cell proliferation is promoted by miR-599 mimics. Transwell assay showed that miR-599 mimics promoted the invasion and migration of NSCLC cells. Luciferase assays confirmed that miR-599 directly binds to the 3'untranslated region of SATB2, and western blotting showed that miR-599 suppresses the expression of SATB2 at the protein level. This study indicates that miR-599 promotes proliferation and invasion of NSCLC cell lines via SATB2. The miR-599 may represent a potential therapeutic target for NSCLC treatment.
Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.
Wenjun
W
Tian
Department of Respiratory Medicine, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, China.
Guanghai
G
Wang
Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.
Yiqing
Y
Liu
Key Laboratory of Medical Diagnostics of Ministry of Education, Faculty of Laboratorial Medicine, Chongqing Medical University, Chongqing, China.
Zhenglan
Z
Huang
Department of Respiratory Medicine, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, China.
Caiqing
C
Zhang
Department of Respiratory Medicine, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, China.
Kang
K
Ning
Department of Respiratory Medicine, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, China.
Cuixiang
C
Yu
Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.
Yajuan
Y
Shen
Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.
Minghui
M
Wang
Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.
Yuantang
Y
Li
Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.
Yong
Y
Wang
Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.
Bingchang
B
Zhang
Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.
Yaoran
Y
Zhao
D020413:3' Untranslated Regions; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008168:Lung / Q000378:metabolism / Q000473:pathology*; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D036961:Matrix Attachment Region Binding Proteins / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics*; D015854:Up-Regulation
D020413:3' Untranslated Regions; C000608803:MIRN599 microRNA, human; D036961:Matrix Attachment Region Binding Proteins; D035683:MicroRNAs; C521297:SATB2 protein, human; D014157:Transcription Factors
NSCLC; SATB2; miR-599