A phosphoinositide-specific phospholipase C pathway elicits stress-induced Ca2+signals and confers salt tolerance to rice.
In animal cells, phospholipase C (PLC) isoforms predominantly hydrolyze phosphatidylinositol-4,5-biphosphates [PtdIns(4,5)P2] into the second messengers diacylglycerol (DAG) and inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] to regulate diverse biological processes. By contrast, the molecular mechanisms and physiological significance of PLC signaling in plants still awaits full elucidation. Here, we identified a rice (Oryza sativa cv) PI-PLC, OsPLC1, which preferred to hydrolyze phosphatidylinositol-4-phosphate (PtdIns4P) and elicited stress-induced Ca2+signals regulating salt tolerance. Analysis by ion chromatography revealed that the concentration of PtdIns4P was c. 28 times of that of PtdIns(4,5)P2in shoots. OsPLC1 not only converted PtdIns(4,5)P2but also - and even more efficiently - converted PtdIns4P into DAG and Ins(1,4,5)P3in vitro and in vivo. Salt stress induced the recruitment of OsPLC1 from cytoplasm to plasma membrane, where it hydrolyzed PtdIns4P. The stress-induced Ca2+signaling was dependent on OsPLC1, and the PLC-mediated Ca2+signaling was essential for controlling Na+accumulation in leaf blades, thus establishing whole plant salt tolerance. Our work identifies a conversion pathway and physiological function for PtdIns4P pools in rice and reveals the connection between phosphoinositides and Ca2+signals mediated by PLC during salt stress responses.
College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.
Li
L
Li
College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.
Fawei
F
Wang
College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.
Peiwen
P
Yan
College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.
Wen
W
Jing
Institut für Biologie und Biotechnologie der Pflanzen, Universität Münster, Schlossplatz 7, Münster, 48149, Germany.
Chunxia
C
Zhang
Institut für Biologie und Biotechnologie der Pflanzen, Universität Münster, Schlossplatz 7, Münster, 48149, Germany.|College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia.
Jörg
J
Kudla
College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.
Wenhua
W
Zhang
D020013:Calcium Signaling / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D055786:Gene Knockout Techniques; D006868:Hydrolysis; D012275:Oryza / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000502:physiology*; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000378:metabolism; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism; D054801:Phosphoinositide Phospholipase C / Q000378:metabolism*; D018520:Plant Shoots / Q000187:drug effects / Q000378:metabolism; D054046:Plant Stomata / Q000166:cytology / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D055049:Salt Tolerance / Q000187:drug effects / Q000235:genetics; D012964:Sodium / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism
D019269:Phosphatidylinositol 4,5-Diphosphate; D018129:Phosphatidylinositol Phosphates; C037178:phosphatidylinositol 4-phosphate; D012965:Sodium Chloride; D012964:Sodium; D054801:Phosphoinositide Phospholipase C
Ca2+ signal; phosphatidylinositol-4-phosphate; phospholipase C; rice; salt response