Tracking stem cells with superparamagnetic iron oxide nanoparticles: perspectives and considerations.
Superparamagnetic iron oxide nanoparticles (SPIONs) have been used for diagnoses in biomedical applications, due to their unique properties and their apparent safety for humans. In general, SPIONs do not seem to produce cell damage, although their long-term in vivo effects continue to be investigated. The possibility of efficiently labeling cells with these magnetic nanoparticles has stimulated their use to noninvasively track cells by magnetic resonance imaging after transplantation. SPIONs are attracting increasing attention and are one of the preferred methods for cell labeling and tracking in preclinical and clinical studies. For clinical protocol approval of magnetic-labeled cell tracking, it is essential to expand our knowledge of the time course of SPIONs after cell incorporation and transplantation. This review focuses on the recent advances in tracking SPION-labeled stem cells, analyzing the possibilities and limitations of their use, not only focusing on myocardial infarction but also discussing other models.
NUMPEX-Bio, Federal University of Rio de Janeiro, Duque de Caxias, RJ.
Jasmin
Laboratory of Animal Reproduction, Embrapa Dairy Cattle, Juiz de Fora, MG; Laboratory of Genetics, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
Gustavo Torres
GT
de Souza
Institute Gustave-Roussy of Oncology, Paris-Sud University, Villejuif, France.
Ruy Andrade
RA
Louzada
Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, RJ.
Paulo Henrique
PH
Rosado-de-Castro
Institute Carlos Chagas Filho of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Rosalia
R
Mendez-Otero
Institute Carlos Chagas Filho of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Antonio Carlos
AC
Campos de Carvalho
D000818:Animals; D058948:Cell Tracking / Q000379:methods*; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D013234:Stem Cells / Q000737:chemistry* / Q000166:cytology*
D058185:Magnetite Nanoparticles
cell tracking; in vivo imaging; myocardial infarction; nanoparticles; stem cells; superparamagnetic iron oxide nanoparticles