Effect of Omegaven on mast cell concentration in diabetic wound healing.
Diabetic wound healing is a complicated process. In all over the world 15% of 200 million diabetic people suffer from diabetic foot problems. Mast cells are known to participate in three phases of wound healing: the inflammatory reaction, angiogenesis and extracellular-matrix reabsorption. The inflammatory reaction is mediated by released histamine and arachidonic acid metabolites. Omega-3 fatty acids alter proinflammatory cytokine production during wound healing which affects the presence of inflammatory cells in wound area as well, but how this events specifically influences the presence of mast cells in wound healing is not clearly understood. This study is conducted to determine the effect of Omegaven, eicosapentaenoic (EPA) and docosahexaenoic (DHA) on pattern of presence of mast cells in diabetic wound area. Diabetic male wistar rats were euthanized at 1, 3, 5, 7 and 15 days after the excision was made. To estimate the number of mast cells histological sections were provided from wound area and stained with toluidine blue. In this relation wound area (8400 microscopic field, 45.69 mm2) were examined by stereological methods by light microscope. We found that comparing experimental and control group, omega-3 fatty acids significantly decreased wound area in day 7 and also the number of grade three mast cells in day 3 and 5. We also found that wound strength has significantly increased in experimental group at day 15.
Department of Anatomical Sciences, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran. Electronic address: babaie@arakmu.ac.ir.
Saeid
S
Babaei
Department of Biochemistry and Nutrition, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran. Electronic address: Ansari@arakmu.ac.ir.
Hadi
H
Ansarihadipour
Department of Biochemistry and Nutrition, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran. Electronic address: NAKHAEIMR@gmail.com.
Mahmoodreza
M
Nakhaei
Department of Anatomical Sciences, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran. Electronic address: M_darabi36@yahoo.com.
Mohammadreza
M
Darabi
Department of Anatomical Sciences, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran. Electronic address: Dr.Bayat@arakmu.ac.ir.
Parvindokht
P
Bayat
Department of Anatomical Sciences, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran. Electronic address: sakhaieh@gmail.com.
Mohammadhassan
M
Sakhaei
Department of Anatomical Sciences, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran. Electronic address: Dr.baazm@arakmu.ac.ir.
Maryam
M
Baazm
Department of Anatomical Sciences, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran. Electronic address: Mohammadhoseini_a@yahoo.com.
Atefe
A
Mohammadhoseiny
D000818:Animals; D002452:Cell Count; D048909:Diabetes Complications / Q000188:drug therapy*; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced; D005395:Fish Oils / Q000008:administration & dosage*; D008297:Male; D008407:Mast Cells / Q000187:drug effects*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides; D014945:Wound Healing / Q000187:drug effects*
D005395:Fish Oils; D014280:Triglycerides; C568345:fish oil triglycerides
Diabetes; Mast cell; Omegaven; Wound healing