An association between overexpression of DNA methyltransferase 3B4 and clear cell renal cell carcinoma.
It is well known that abnormal DNA methylations occur frequently in kidney cancer. However, it remains unclear exactly which types of DNA methyltransferases (DNMT) contribute to the pathologies of kidney cancers. In order to determine the functions of DNA methyltransferase in kidney tumorigenesis on the molecular level, we examined the mRNA expression levels of DNMT1, DNMT3A, DNMT3B, and DNMT3B variants in renal cell carcinoma tissue. Both mRNA and protein levels of DNMT3B4, a splice variant of DNMT3B, were increased in renal cell carcinoma tissue compared with adjacent control tissues. Additionally, Alu elements and long interspersed nuclear elements (LINE-1) were hypomethylated in renal cell carcinoma tissue. Meanwhile, methylation of the promoter for RASSF1A, a tumor suppressor gene, was moderately increased in renal cell carcinoma tissue, while RASSF1A expression was decreased. Thus, our data suggest that the overexpression of DNMT3B4 may play an important role in human kidney tumorigenesis through chromosomal instability and methylation of RASSF1A.
Biomedicine Research Center and Basic Medical College, Baotou Medical College, Inner Mongolia, PRC.
You
Y
Liu
Biomedicine Research Center and Basic Medical College, Baotou Medical College, Inner Mongolia, PRC.
Liantao
L
Sun
Department of Neurology, University of California San Francisco, San Francisco, CA, USA.
Peter
P
Fong
Biomedicine Research Center and Basic Medical College, Baotou Medical College, Inner Mongolia, PRC.
Jie
J
Yang
Biomedicine Research Center and Basic Medical College, Baotou Medical College, Inner Mongolia, PRC.
Zhuxia
Z
Zhang
Biomedicine Research Center and Basic Medical College, Baotou Medical College, Inner Mongolia, PRC.
Shuihui
S
Yin
Biomedicine Research Center and Basic Medical College, Baotou Medical College, Inner Mongolia, PRC.
Shuyuan
S
Jiang
Biomedicine Research Center and Basic Medical College, Baotou Medical College, Inner Mongolia, PRC.
Xiaolei
X
Liu
Biomedicine Research Center and Basic Medical College, Baotou Medical College, Inner Mongolia, PRC.
Hongge
H
Ju
Biomedicine Research Center and Basic Medical College, Baotou Medical College, Inner Mongolia, PRC.
Lihua
L
Huang
Biomedicine Research Center and Basic Medical College, Baotou Medical College, Inner Mongolia, PRC.
Jing
J
Bai
Department of Oral and Maxillofacial Surgery, University of California San Francsico, San Francisco, USA.
Kerui
K
Gong
Biomedicine Research Center and Basic Medical College, Baotou Medical College, Inner Mongolia, PRC.
Shaochun
S
Yan
Department of Neurology, First Affiliated Hospital of Baotou Medical College, Inner Mongolia, PRC.
Chunyang
C
Zhang
Biomedicine Research Center and Basic Medical College, Baotou Medical College, Inner Mongolia, PRC.|Institute for Hypoxia Medicine, Capital Medical University, Beijing, PRC.
Guo
G
Shao
D000328:Adult; D000368:Aged; D015153:Blotting, Western; D002292:Carcinoma, Renal Cell / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics* / Q000378:metabolism; D019175:DNA Methylation; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007680:Kidney Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism; D000097584:DNA Methyltransferase 3B
D004248:DNA (Cytosine-5-)-Methyltransferases; D025521:Tumor Suppressor Proteins; D000097584:DNA Methyltransferase 3B; C411762:RASSF1 protein, human
DNA methyltransferase 3B; clear cell renal cell carcinoma