Chromatographic separation and detection of contaminants from whole milk powder using a chitosan-modified silver nanoparticles surface-enhanced Raman scattering device.
Methods for the on-site analysis of food contaminants are in high demand. Although portable Raman spectroscopy is commonly used to test food on-site, it can be challenge to achieve this goal with rapid detection and inexpensive substrate. In this study, we detected trace food contaminants in samples of whole milk powder using the methods that combined chromatography with surface-enhanced Raman scattering detection (SERS). We developed a simple and efficient technique to fabricate the paper with chitosan-modified silver nanoparticles as a SERS-active substrate. The soaking time of paper and the concentration of chitosan solution were optimized for chromatographic separation and SERS detection. We then studied the separation properties for real applications including complex sample matrices, and detected melamine at 1mg/L, dicyandiamide at 100mg/L and sodium sulfocyanate at 10mg/L in whole milk powder. As such, our methods have great potential for field-based detection of milk contaminants.
Department of Pharmacy, Shanghai Chang Hai Hospital, Second Military Medical University, China; Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, China.
Dan
D
Li
Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, China.
Di Y
DY
Lv
Department of Pharmacy, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, China.
Qing X
QX
Zhu
Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, China.
Hao
H
Li
Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, China.
Hui
H
Chen
Department of Pharmaceutical Analysis, School of Pharmacy, Fujian University of Traditional Chinese Medicine School of Pharmacy, China; Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, China.
Mian M
MM
Wu
Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, China.
Yi F
YF
Chai
Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, China. Electronic address: fenglufeng@hotmail.com.
Feng
F
Lu
D000818:Animals; D048271:Chitosan / Q000737:chemistry*; D002845:Chromatography; D005506:Food Contamination / Q000032:analysis*; D006146:Guanidines / Q000032:analysis; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D008892:Milk / Q000737:chemistry*; D011208:Powders / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D012996:Solutions; D013059:Spectrum Analysis, Raman; D014227:Triazines / Q000032:analysis
D006146:Guanidines; D011208:Powders; D012996:Solutions; D014227:Triazines; D012834:Silver; D048271:Chitosan; C004711:dicyandiamido; C011907:melamine
Chitosan-modified silver nanoparticles paper; Contaminants; SERS; Whole milk powder