Effect of Viscum schimperi on advanced glycation endproducts formation.
Diabetes is a disease characterized by elevated uncontrolled glucose level. Hyperglycemia results in diabetic complication due to a reaction between sugar and amino acid of proteins to form advanced glycation endproducts (AGEs) in different tissues. Medicinal plants are considered as a great source of bioactive compounds that affect many ailments. In this regard; AGEs formation could be inhibited by many bioactive compounds isolated from medicinal plants. Viscum schimperi Engl. is a plant belongs to Loranthaceae and known for its antidiabetic activity. In this study; total methanol extract of V. schimperi (VT) was prepared, suspended in water and subjected to fractionation with chloroform followed by n-butanol to give (VC) and (VB) fractions respectively. The aqueous mother liquor was evaporated to form (VA) fraction. The inhibitory effect of all prepared fraction on the formation of advanced glycation endproducts (AGEs) was studied. The results revealed that V. schimperi extract and its different fractions inhibited protein glycation and oxidation of BSA induced by ribose together with decrease of protein aggregation. In conclusion; V. schimperi will be useful in management of diabetic complications based on its inhibition of advanced glycation endproduct formation.
Department of Natural Products, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia / Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Hossam Mohamed
HM
Abdallah
Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia.
Doha Omar
DO
ALGhamdi
Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia.
Maab Siddiq
MS
Al-Salem
Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia.
Maryam
M
Alattas
Department of Pharmacology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia.
Hany Mohamed
HM
El-Bassossy
Department of Clinical Pharmacy, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia.
Abdulrahman Mohammed
AM
Alahdal
Department of Natural Products, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia.
Ibrahim Ateya
IA
Shehata
Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Essam
E
Abdel-Sattar
D020001:1-Butanol / Q000737:chemistry; D002725:Chloroform / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000737:chemistry; D017127:Glycation End Products, Advanced / Q000737:chemistry*; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000432:Methanol / Q000737:chemistry; D008517:Phytotherapy; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D066329:Protein Aggregates; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D028181:Viscum / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D017127:Glycation End Products, Advanced; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D066329:Protein Aggregates; D012997:Solvents; C081628:advanced glycation end products-bovine serum albumin; D014867:Water; D012710:Serum Albumin, Bovine; D002725:Chloroform; D020001:1-Butanol; D005947:Glucose; D000432:Methanol