Turning single-molecule localization microscopy into a quantitative bioanalytical tool.
Single-molecule localization microscopy (SMLM) generates super-resolution images by serially detecting individual fluorescent molecules. The power of SMLM, however, goes beyond images: biologically relevant information can be extracted from the mathematical relationships between the positions of the fluorophores in space and time. Here we review the history of SMLM and how recent progress in methods for spatial point analysis has enabled quantitative measurement of SMLM data, providing insights into biomolecule patterning, clustering and oligomerization in biological systems.
ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales, Sydney, Australia.|EMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales, Sydney, Australia.
Philip R
PR
Nicovich
Department of Physics and Randall Division of Cell and Molecular Biophysics, King's College London, London, UK.
Dylan M
DM
Owen
ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales, Sydney, Australia.|EMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales, Sydney, Australia.
Katharina
K
Gaus
D002623:Chemistry Techniques, Analytical / Q000379:methods*; D000072760:Single Molecule Imaging / Q000379:methods*