Structure of an octameric form of the minichromosome maintenance protein from the archaeon Pyrococcus abyssi.
Cell division is a complex process that requires precise duplication of genetic material. Duplication is concerted by replisomes. The Minichromosome Maintenance (MCM) replicative helicase is a crucial component of replisomes. Eukaryotic and archaeal MCM proteins are highly conserved. In fact, archaeal MCMs are powerful tools for elucidating essential features of MCM function. However, while eukaryotic MCM2-7 is a heterocomplex made of different polypeptide chains, the MCM complexes of many Archaea form homohexamers from a single gene product. Moreover, some archaeal MCMs are polymorphic, and both hexameric and heptameric architectures have been reported for the same polypeptide. Here, we present the structure of the archaeal MCM helicase from Pyrococcus abyssi in its single octameric ring assembly. To our knowledge, this is the first report of a full-length octameric MCM helicase.
Institute of Molecular, Cell and Systems Biology, University of Glasgow, University Avenue, Glasgow G12 8QQ, UK.|School of Biological Sciences and Max Born Crescent, Edinburgh EH9 3JR, UK.|Centre for Science at extreme conditions, University of Edinburgh, Max Born Crescent, Edinburgh EH9 3JR, UK.
Giuseppe
G
Cannone
Institute of Molecular, Cell and Systems Biology, University of Glasgow, University Avenue, Glasgow G12 8QQ, UK.|School of Biological Sciences and Max Born Crescent, Edinburgh EH9 3JR, UK.|ISIS neutron source, Science and Technologies Research Council, Rutherford Appleton Laboratories, Harwell, OX11 0QX United Kingdom.
Silvia
S
Visentin
IFREMER, Laboratoire de Microbiologie des Environnements Extrêmes, UMR 6197, ZI de la pointe du diable CS 10070 29280 Plouzané, France.|Université de Bretagne Occidentale, Laboratoire de Microbiologie des Environnements Extrêmes, UMR6197, rue Dumont d'Urville 29280 Plouzané, France.|CNRS, Laboratoire de Microbiologie des Environnements Extrêmes, UMR6197, rue Dumont d'Urville 29280 Plouzané, France.
Adeline
A
Palud
IFREMER, Laboratoire de Microbiologie des Environnements Extrêmes, UMR 6197, ZI de la pointe du diable CS 10070 29280 Plouzané, France.|Université de Bretagne Occidentale, Laboratoire de Microbiologie des Environnements Extrêmes, UMR6197, rue Dumont d'Urville 29280 Plouzané, France.|CNRS, Laboratoire de Microbiologie des Environnements Extrêmes, UMR6197, rue Dumont d'Urville 29280 Plouzané, France.
Ghislaine
G
Henneke
Institute of Molecular, Cell and Systems Biology, University of Glasgow, University Avenue, Glasgow G12 8QQ, UK.
Laura
L
Spagnolo
D020285:Cryoelectron Microscopy; D064110:Minichromosome Maintenance Proteins / Q000378:metabolism* / Q000648:ultrastructure*; D055503:Protein Multimerization; D043903:Pyrococcus abyssi / Q000201:enzymology*
D064110:Minichromosome Maintenance Proteins