Novel Pyrimidine Tagged Silver Nanoparticle Based Fluorescent Immunoassay for the Detection of Pseudomonas aeruginosa.
A simple pyrimidine-based fluorescent probe (R)-4-(anthracen-9-yl)-6- (naphthalen-1-yl)-1,6-dihydropyrimidine-2-amine (ANDPA) was synthesized through the greener one pot reaction and characterized by IR, NMR, and ESI-Mass. Glucose stabilized silver nanoparticles (Glu-AgNPs) were also synthesized and characterized using UV, IR, XRD, SEM, and TEM. When ANDPA was tagged with Glu-AgNPs, the fluorescent intensity of ANDPA decreased drastically. When the monoclonal antibody (Ab) [immunoglobulin G (IgG)] of Pseudomonas aeruginosa (PA) was attached with ANDPA/Glu-AgNPs, the original intensity of the probe was recovered with minimal enhancement at 446 nm. On further attachment of PA with ANDPA/Glu-AgNPs/PA, the fluorescence intensity of the probe was enhanced obviously at 446 nm with red shift. This phenomenon was further supported by SEM and TEM. The linear range of detection is from 8 to 10-1CFU/mL, and LOD is 1.5 CFU/mL. The immunosensor was successfully demonstrated to detect Pseudomonas aeruginosa in water, soil, and food products like milk, sugar cane, and orange juices.
Department of Natural Products Chemistry, School of Chemistry, Madurai Kamaraj University , Madurai-625 021, Tamilnadu, India.
Sundaram
S
Ellairaja
Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University , Madurai-625 021, Tamilnadu, India.
Narayanaswamy
N
Krithiga
Biotechnology Division, Defence Research Development & Establishment , Jhansi Road, Gwalior 474002, Madhya Pradesh, India.
Sarkaraisamy
S
Ponmariappan
Department of Natural Products Chemistry, School of Chemistry, Madurai Kamaraj University , Madurai-625 021, Tamilnadu, India.
Vairathevar Sivasamy
http://orcid.org/0000-0002-5991-5091
VS
Vasantha
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D005453:Fluorescence; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000302:isolation & purification; D011743:Pyrimidines / Q000737:chemistry*; D012834:Silver / Q000737:chemistry
D011743:Pyrimidines; D012834:Silver; C030986:pyrimidine
Pseudomonas aeruginosa; aggregation; fluorescence; immunosensor; nanoparticles; pyrimidine