Rapid synthesis of titanium(IV)-immobilized magnetic mesoporous silica nanoparticles for endogenous phosphopeptides enrichment.
The MALDI-TOF MS has already been a main platform for phosphoproteome analysis. However, there are some weaknesses in direct analysis of endogenous phosphopeptides by MALDI-TOF MS because of the serious suppression effect and poor ionization efficiency, which is brought by the excess of nonphosphopeptides and protein. It is essential to enrich endogenous phosphopeptides from complex biosamples efficiently prior to MALDI-TOF MS analysis. Herein, we present a time-saving and detailed protocol for the synthesis of titanium(iv)-immobilized magnetic mesoporous silica nanoparticles (denoted as Fe3O4@mSiO2-Ti4+), the subsequent enrichment process, and MALDI-TOF MS analysis. We tested the LOD, size-exclusive effect, reproducibility, and stability of Fe3O4@mSiO2-Ti4+nanoparticles. Furthermore, the ability of this protocol for identifying endogenous phosphopeptides in healthy human serum and saliva was investigated.
Department of Chemistry and Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai, P. R., China.
Jizong
J
Yao
Department of Chemistry and Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai, P. R., China.
Nianrong
N
Sun
Department of Chemistry and Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai, P. R., China.
Jiawen
J
Wang
Department of Chemistry and Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai, P. R., China.
Yiqin
Y
Xie
Department of Chemistry and Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai, P. R., China.
Chunhui
C
Deng
Department of Chemistry and Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai, P. R., China.
Xiangmin
X
Zhang
D002364:Caseins / Q000737:chemistry; D060326:Chemistry Techniques, Synthetic; D006801:Humans; D057230:Limit of Detection; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D010748:Phosphopeptides / Q000032:analysis* / Q000097:blood; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry; D014025:Titanium / Q000737:chemistry*; D014961:X-Ray Diffraction
D002364:Caseins; D058185:Magnetite Nanoparticles; D010748:Phosphopeptides; D012822:Silicon Dioxide; D014025:Titanium
Endogenous phosphopeptides; MALDI-TOF MS; Mesoporous silica; Size exclusive