Ribosomal Intergenic Spacer 1 Based Characterization of Button Mushroom (Agaricus bisporus) Strains.
Breeding the button mushroom requires genetic information about its strains. This study was undertaken to genetically characterize four domestically bred button mushroom strains (Saea, Saejung, Saedo, Saeyeon cultivars) and to assess the possibility of using the intergenic spacer 1 (IGS1) region of rDNA as a genetically variable region in the genetic characterization. For the experiment, 34 strains ofAgaricus bisporus, two strains ofA. bitorquis, and one strain ofA. silvaticus, from 17 countries were used. Nucleotide sequence analysis of IGS1 rDNA in these 37Agaricusstrains confirmed that genetic variations exist, not only among the four domestic strains, but also between the four domestic strains and foreign strains. Crossing two different haploid strains ofA. bisporusseems to generate genetic variation in the IGS1 region in their off-spring haploid strains. Phylogenetic analysis based on the IGS1 sequence revealed allA. bisporusstrains could be differentiated fromA. silvaticusandA. bitorquisstrains. Five genetic groups were resolved amongA. bisporusstrains. Saejung and Saeyeon cultivars formed a separate genetic group. Our results suggest that IGS1 could be complementarily applied in the polymorphism analysis of button mushroom.
Department of Microbiology and Institute of Biodiversity, Dankook University, Cheonan 31116, Korea.
Hyuk Woo
HW
Kwon
Department of Microbiology and Institute of Biodiversity, Dankook University, Cheonan 31116, Korea.
Min Ah
MA
Choi
Department of Microbiology and Institute of Biodiversity, Dankook University, Cheonan 31116, Korea.
Dae Wook
DW
Kim
Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, Korea.
Youn-Lee
YL
Oh
Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, Korea.
Min Woo
MW
Hyun
Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, Korea.
Won-Sik
WS
Kong
Department of Microbiology and Institute of Biodiversity, Dankook University, Cheonan 31116, Korea.
Seong Hwan
SH
Kim
Agaricus bisporus; Dikaryotic strain; Ribosomal intergenic spacer 1