DNA Methylation in Breast Tumor from High-risk Women in the Breast Cancer Family Registry.
To examine DNA methylation profiles in breast tumors of women with a strong breast cancer family history, we measured methylation by bisulfite sequencing in 40 genes in 40 breast tumor tissues from women in the Breast Cancer Family Registry. We selected candidate genes from analysis of the Cancer Genome Atlas project (TCGA) breast data. Compared to TCGA breast cancer, BCFR cases are younger and more likely to be ER-negative. Overall, we found that many of the methylation differences between BCFR tumor and normal adjacent tissues were smaller than that in TCGA samples. We found only 32% of tested genes were hypermethylated in BCFR; the largest difference was 36.1% for SEPW1, and the smallest difference was 10% for RYR2. These data suggest the importance of examining breast cancer cases including familial cases enriched with early-onset cancers to identify methylation markers that can be examined in blood as biomarkers for early detection.
Department of Environmental Health Sciences, Mailman School of Public Health of Columbia University, New York, NY, U.S.A. hw2057@columbia.edu.
Hui-Chen
HC
Wu
Department of Pathology, The University of Melbourne, Melbourne, Victoria, Australia.
Melissa C
MC
Southey
Department of Pathology and Cell Biology, College of Physicians & Surgeons of Columbia University, New York, NY, U.S.A.
Hanina
H
Hibshoosh
Department of Environmental Health Sciences, Mailman School of Public Health of Columbia University, New York, NY, U.S.A.|Herbert Irving Comprehensive Cancer Center, Columbia University Medical Center, New York, NY, U.S.A.
Regina M
RM
Santella
Herbert Irving Comprehensive Cancer Center, Columbia University Medical Center, New York, NY, U.S.A.|Department of Epidemiology, Mailman School of Public Health of Columbia University, New York, NY, U.S.A.
Mary Beth
MB
Terry
D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D019175:DNA Methylation; D005192:Family Health; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005820:Genetic Testing / Q000379:methods / Q000706:statistics & numerical data; D055106:Genome-Wide Association Study / Q000379:methods / Q000706:statistics & numerical data; D006801:Humans; D008875:Middle Aged; D011960:Receptors, Estrogen / Q000378:metabolism; D012042:Registries / Q000706:statistics & numerical data*
D011960:Receptors, Estrogen
Breast cancer; DNA methylation; TCGA; epigenetics; promoter DNA methylation