Molecular Phylogeny and Morphology ofMycosphaerella nawae, the Causal Agent of Circular Leaf Spot on Persimmon.
In this study, the phylogeny and morphology ofMycosphaerella nawae(Dothideomycetes, Ascomycota) were examined using Korean and Japanese isolates, to establish the phylogenetic relationship betweenM. nawaeand its allied species. Korean and Japanese isolates ofM. nawaewere collected from circular leaf spot-diseased leaves and were confirmed based on internal transcribed spacer (ITS) sequence data. Phylogenetic analysis was conducted using multiple genes, including the ITS region, 28S rDNA, β-tubulin, translation elongation factor-1α, and actin genes. Our results revealed thatM. nawaeis closely related to members of the genusPhaeophleosporabut are distant from theRamulariaspp. In addition, microscopic analysis revealed pseudothecia on the adaxial and abaxial surface of overwintered diseased leaves (ODL) and only on the abaxial surface of diseased leaves. Ascospores are oval to fusiform, one-septate, tapered at both ends, 1.7~3.1 × 8.1~14.1 µm, and were observed in ODL. Conidia are oval, guttulate, one-septate, 3.5~4.9 × 12.8~19.8 µm, and barely discernable on 30-day cultures. To our knowledge, this is the first report on the phylogeny ofM. nawae, which is closely related to the genusPhaeophleospora, especiallyP. scytalidii.
College of Agriculture and Life Sciences, Kyungpook National University, Daegu 41566, Korea.
Seung-Yeol
SY
Lee
Persimmon Experiment Station, Gyeongsangbuk-do Agricultural Research & Extension Services, Sangju 37268, Korea.
Yang-Sook
YS
Lim
College of Agriculture and Life Sciences, Kyungpook National University, Daegu 41566, Korea.; Institute of Plant Medicine, Kyungpook National University, Daegu 41566, Korea.
Hee-Young
HY
Jung
Persimmon; Phaeophleospora spp.; Phylogenetic analysis