Mining pathway associations for disease-related pathway activity analysis based on gene expression and methylation data.
BACKGROUND
The problem of discovering genetic markers as disease signatures is of great significance for the successful diagnosis, treatment, and prognosis of complex diseases. Even if many earlier studies worked on identifying disease markers from a variety of biological resources, they mostly focused on the markers of genes or gene-sets (i.e., pathways). However, these markers may not be enough to explain biological interactions between genetic variables that are related to diseases. Thus, in this study, our aim is to investigate distinctive associations among active pathways (i.e., pathway-sets) shown each in case and control samples which can be observed from gene expression and/or methylation data.


RESULTS
The pathway-sets are obtained by identifying a set of associated pathways that are often active together over a significant number of class samples. For this purpose, gene expression or methylation profiles are first analyzed to identify significant (active) pathways via gene-set enrichment analysis. Then, regarding these active pathways, an association rule mining approach is applied to examine interesting pathway-sets in each class of samples (case or control). By doing so, the sets of associated pathways often working together in activity profiles are finally chosen as our distinctive signature of each class. The identified pathway-sets are aggregated into a pathway activity network (PAN), which facilitates the visualization of differential pathway associations between case and control samples. From our experiments with two publicly available datasets, we could find interesting PAN structures as the distinctive signatures of breast cancer and uterine leiomyoma cancer, respectively.


CONCLUSIONS
Our pathway-set markers were shown to be superior or very comparable to other genetic markers (such as genes or gene-sets) in disease classification. Furthermore, the PAN structure, which can be constructed from the identified markers of pathway-sets, could provide deeper insights into distinctive associations between pathway activities in case and control samples.
Bio-Intelligence & Data Mining Laboratory, Graduate School of Electronics Engineering, Kyungpook National University, 80, Daehak-ro, Buk-gu, Daegu, 41566 Republic of Korea.
Hyeonjeong
H
Lee
School of Electronics Engineering, Kyungpook National University, 80, Daehak-ro, Buk-gu, Daegu, 41566 Republic of Korea.
Miyoung
M
Shin
Biomarker discovery; Gene expression analysis; Pathway association mining; Pathway association network; Pathway-set markers