Measuring acne using Coproporphyrin III, Protoporphyrin IX, and lesion-specific inflammation: an exploratory study.
Propionibacterium acnes: (P. acnes) produce Porphyrins; however, fluorescence measurement of Porphyrins from Ultraviolet-A (UVA) images has failed to establish a correlation. Acne clinical research and imaging has ignored the spectral excitation-emission characteristics and the exact pattern of the Porphyrins synthesized by P. acnes. In this exploratory study, for the first time, the possible relationships of Coproporphyrin III (CpIII) and Protoporphyrin IX (PpIX) fluorescence as well as acne lesion-specific inflammation measurements with clinical signs of acne are investigated. Furthermore, the sensitivity of these measurements in tracking and differentiating the known treatment effects of Benzoyl Peroxide (BPO) 5%, and combination of Clindamycin + BPO are also evaluated. Comedonal and papulopustular lesions identified by investigators during a live assessment of 24 mild-to-severe acne subjects were compared with fluorescence and inflammation measurements obtained from analysis of VISIA®-CR images. CpIII fluorescence spots showed a strong correlation (r = 0.69-0.83), while PpIX fluorescence spots showed a weak correlation (r = 0.19-0.27) with the investigators' comedonal lesion counts. A strong correlation was also observed between the investigators' papulopustular lesion counts and acne lesion-specific inflammation (r = 0.76). Our results suggest that CpIII fluorescence and acne lesion-specific-inflammation measurement can provide objective indication of comedonal and papulopustular acne severity, respectively. Furthermore, these measurements may be more sensitive and specific in evaluating treatment effects and early signs of acne lesion progression compared to investigators' lesion counts.
Canfield Scientific Inc., 4 Wood Hollow Road, Parsippany, NJ, 07054, USA. Sachin.Patwardhan@CanfieldSci.com.
Sachin V
SV
Patwardhan
Department of Dermatology and Allergy, Clinical Research Center for Hair and Skin Science, Charité-Universitätsmedizin Berlin, Berlin, Germany.
C
C
Richter
Department of Dermatology and Allergy, Clinical Research Center for Hair and Skin Science, Charité-Universitätsmedizin Berlin, Berlin, Germany.
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A
Vogt
Department of Dermatology and Allergy, Clinical Research Center for Hair and Skin Science, Charité-Universitätsmedizin Berlin, Berlin, Germany.
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U
Blume-Peytavi
Canfield Scientific Inc., 4 Wood Hollow Road, Parsippany, NJ, 07054, USA.
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D
Canfield
Department of Dermatology and Allergy, Clinical Research Center for Hair and Skin Science, Charité-Universitätsmedizin Berlin, Berlin, Germany.
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J
Kottner
D000152:Acne Vulgaris / Q000000981:diagnostic imaging* / Q000188:drug therapy*; D000293:Adolescent; D000328:Adult; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D001585:Benzoyl Peroxide / Q000627:therapeutic use; D002981:Clindamycin / Q000627:therapeutic use; D003306:Coproporphyrins / Q000494:pharmacology*; D003879:Dermatologic Agents / Q000627:therapeutic use*; D004359:Drug Therapy, Combination; D005260:Female; D006801:Humans; D008297:Male; D061848:Optical Imaging / Q000379:methods*; D011166:Porphyrins / Q000378:metabolism; D011425:Propionibacterium acnes / Q000378:metabolism; D011524:Protoporphyrins / Q000494:pharmacology*; D012720:Severity of Illness Index; D014449:Tyrothricin / Q000627:therapeutic use*; D055815:Young Adult
D000900:Anti-Bacterial Agents; D003306:Coproporphyrins; D003879:Dermatologic Agents; D011166:Porphyrins; D011524:Protoporphyrins; D014449:Tyrothricin; C026971:coproporphyrin III; D002981:Clindamycin; C028025:protoporphyrin IX; D001585:Benzoyl Peroxide
Acne assessment; Acne inflammation; Coproporphyrin III; Fluorescence imaging; Protoporphyrin IX