Quantification and Qualitative Effects of Different PEGylations on Poly(butyl cyanoacrylate) Nanoparticles.
Protein adsorption on nanoparticles (NPs) used in nanomedicine leads to opsonization and activation of the complement system in blood, which substantially reduces the blood circulation time of NPs. The most commonly used method to avoid protein adsorption is to coat the NPs with polyethylene glycol, so-called PEGylation. Although PEGylation is of utmost importance for designing the in vivo behavior of the NP, there is still a considerable lack of methods for characterization and fundamental understanding related to the PEGylation of NPs. In this work we have studied four different poly(butyl cyanoacrylate) (PBCA) NPs, PEGylated with different types of PEG-based nonionic surfactants-Jeffamine M-2070, Brij L23, Kolliphor HS 15, Pluronic F68-or combinations thereof. We evaluated the PEGylation, both quantitatively by nuclear magnetic resonance (NMR), thermogravimetric analysis (TGA), and time-of-flight secondary ion mass spectrometry (ToF-SIMS) and qualitatively by studying ζ-potential, protein adsorption, diffusion, cellular interactions, and blood circulation half-life. We found that NMR and ToF-SIMS are complementary methods, while TGA is less suitable to quantitate PEG on polymeric NPs. It was found that longer PEG increases both blood circulation time and diffusion of NPs in collagen gels.
Department of Physics, Norwegian University of Science and Technology (NTNU) , Trondheim, Norway.
Andreas K O
0000-0001-5222-9307
AKO
Åslund
Department of Physics, Norwegian University of Science and Technology (NTNU) , Trondheim, Norway.|SINTEF Materials and Chemistry , Trondheim, Norway.
Einar
E
Sulheim
Department of Physics, Norwegian University of Science and Technology (NTNU) , Trondheim, Norway.
Sofie
S
Snipstad
Pharmaceutical Sciences Laboratory, Åbo Akademi University , Turku, Finland.
Eva
E
von Haartman
Department of Physics, Norwegian University of Science and Technology (NTNU) , Trondheim, Norway.
Habib
H
Baghirov
School of Pharmacy, The University of Nottingham , Nottingham NG7 2RD, United Kingdom.
Nichola
0000-0001-5497-8926
N
Starr
Department of Physics, Norwegian University of Science and Technology (NTNU) , Trondheim, Norway.
Mia
M
Kvåle Løvmo
Department of Physics, Norwegian University of Science and Technology (NTNU) , Trondheim, Norway.
Sylvie
S
Lelú
School of Pharmacy, The University of Nottingham , Nottingham NG7 2RD, United Kingdom.
David
D
Scurr
Department of Physics, Norwegian University of Science and Technology (NTNU) , Trondheim, Norway.
Catharina de Lange
CL
Davies
SINTEF Materials and Chemistry , Trondheim, Norway.
Ruth
R
Schmid
SINTEF Materials and Chemistry , Trondheim, Norway.
Ýrr
Ý
Mørch
D004659:Enbucrilate / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008689:Methacrylates / Q000737:chemistry; D050997:Nanomedicine / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry; D013818:Thermogravimetry
D008689:Methacrylates; D011108:Polymers; D013501:Surface-Active Agents; C116863:poly(butyl methacrylate); D004659:Enbucrilate
NMR; PACA; PEG; TGA; ToF-SIMS; circulation time; protein adsorption