In Silico Identification of Proteins Associated with Drug-induced Liver Injury Based on the Prediction of Drug-target Interactions.
Drug-induced liver injury (DILI) is the leading cause of acute liver failure as well as one of the major reasons for drug withdrawal from clinical trials and the market. Elucidation of molecular interactions associated with DILI may help to detect potentially hazardous pharmacological agents at the early stages of drug development. The purpose of our study is to investigate which interactions with specific human protein targets may cause DILI. Prediction of interactions with 1534 human proteins was performed for the dataset with information about 699 drugs, which were divided into three categories of DILI: severe (178 drugs), moderate (310 drugs) and without DILI (211 drugs). Based on the comparison of drug-target interactions predicted for different drugs' categories and interpretation of those results using clustering, Gene Ontology, pathway and gene expression analysis, we identified 61 protein targets associated with DILI. Most of the revealed proteins were linked with hepatocytes' death caused by disruption of vital cellular processes, as well as the emergence of inflammation in the liver. It was found that interaction of a drug with the identified targets is the essential molecular mechanism of the severe DILI for the most of the considered pharmaceuticals. Thus, pharmaceutical agents interacting with many of the identified targets may be considered as candidates for filtering out at the early stages of drug research.
Institute of Biomedical Chemistry 10 building 8, Pogodinskaya str., 119121, Moscow, Russia.|Pirogov Russian National Research Medical University, Medico-Biological Faculty 1, Ostrovitianova str., 117997, Moscow, Russia.
Sergey
S
Ivanov
Institute of Biomedical Chemistry 10 building 8, Pogodinskaya str., 119121, Moscow, Russia.|Pirogov Russian National Research Medical University, Medico-Biological Faculty 1, Ostrovitianova str., 117997, Moscow, Russia.
Maxim
M
Semin
Institute of Biomedical Chemistry 10 building 8, Pogodinskaya str., 119121, Moscow, Russia.|Pirogov Russian National Research Medical University, Medico-Biological Faculty 1, Ostrovitianova str., 117997, Moscow, Russia.
Alexey
A
Lagunin
Institute of Biomedical Chemistry 10 building 8, Pogodinskaya str., 119121, Moscow, Russia.
Dmitry
D
Filimonov
Institute of Biomedical Chemistry 10 building 8, Pogodinskaya str., 119121, Moscow, Russia.
Vladimir
V
Poroikov
D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism; D019295:Computational Biology / Q000379:methods*; D003198:Computer Simulation; D030541:Databases, Genetic; D062313:Databases, Pharmaceutical; D006801:Humans; D008024:Ligands; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D021281:Quantitative Structure-Activity Relationship; D012984:Software
D008024:Ligands; D011506:Proteins
Hepatotoxicity; bioinformatics; off-target; pathway analysis; structure-activity relationships