Coprecipitation-assisted coacervative extraction coupled to high-performance liquid chromatography: An approach for determining organophosphorus pesticides in water samples.
An analytical methodology based on coprecipitation-assisted coacervative extraction coupled to HPLC-UV was developed for determination of five organophosphorus pesticides (OPPs), including fenitrothion, guthion, parathion, methidathion, and chlorpyrifos, in water samples. It involves a green technique leading to an efficient and simple analytical methodology suitable for high-throughput analysis. Relevant physicochemical variables were studied and optimized on the analytical response of each OPP. Under optimized conditions, the resulting methodology was as follows: an aliquot of 9 mL of water sample was placed into a centrifuge tube and 0.5 mL sodium citrate 0.1 M, pH 4; 0.08 mL Al2(SO4)30.1 M; and 0.7 mL SDS 0.1 M were added and homogenized. After centrifugation the supernatant was discarded. A 700 μL aliquot of the coacervate-rich phase obtained was dissolved with 300 μL of methanol and 20 μL of the resulting solution was analyzed by HPLC-UV. The resulting LODs ranged within 0.7-2.5 ng/mL and the achieved RSD and recovery values were <8% (n = 3) and >81%, respectively. The proposed analytical methodology was successfully applied for the analysis of five OPPs in water samples for human consumption of different locations of Mendoza.
Laboratorio de Química Ambiental, Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales (CCT-CONICET-Mendoza), Mendoza, Argentina.|Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Cuyo, Mendoza, Argentina.
Sabrina B
SB
Mammana
Laboratorio de Química Ambiental, Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales (CCT-CONICET-Mendoza), Mendoza, Argentina.
Paula
P
Berton
Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Cuyo, Mendoza, Argentina.|Instituto de Biología Agrícola de Mendoza (CCT-CONICET-Mendoza), Mendoza, Argentina.|Laboratorio de Cromatografía para Agroalimentos, Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Mendoza, Argentina.
Alejandra B
AB
Camargo
Laboratorio de Química Ambiental, Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales (CCT-CONICET-Mendoza), Mendoza, Argentina.
Gustavo E
GE
Lascalea
Laboratorio de Química Ambiental, Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales (CCT-CONICET-Mendoza), Mendoza, Argentina.|Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Cuyo, Mendoza, Argentina.
Jorgelina C
JC
Altamirano
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D055772:Green Chemistry Technology; D057230:Limit of Detection; D016014:Linear Models; D009943:Organophosphorus Compounds / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D010573:Pesticide Residues / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D015203:Reproducibility of Results; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
D009943:Organophosphorus Compounds; D010573:Pesticide Residues; D014874:Water Pollutants, Chemical
Coprecipitation-assisted coacervative extraction; Green chemistry; HPLC; Organophosphorus pesticides; Water samples