Difference in Antibody Responses toMycobacterium tuberculosisAntigens in Japanese Tuberculosis Patients Infected with the Beijing/Non-Beijing Genotype.
The Beijing genotypeMycobacterium tuberculosis(MTB), notorious for its virulence and predisposition to relapse, could be identified by spoligotyping based on genetic heterogeneity. The plasma samples from 20 cases of Beijing and 16 cases of non-Beijing MTB infected individuals and 24 healthy controls (HCs) were collected, and antibodies against 11 antigens (Rv0679c142Asn, Rv0679c142Lys, Ag85B, Ag85A, ARC, TDM-M, TDM-K, HBHA, MDP-1, LAM, and TBGL) were measured by ELISA. Compared to the HCs, the MTB infected subjects showed higher titers of anti-Ag85B IgG (positivity 58.2%) and anti-ACR IgG (positivity 48.2%). Of note, anti-ACR IgG showed higher titer in Beijing MTB infected tuberculosis (TB) patients than in HC (Kruskal-Wallis test,p< 0.05), while the levels of anti-Ag85B, anti-TBGL, anti-TDM-K, and anti-TDM-M IgG were higher in non-Beijing TB patients than in HC. Moreover, anti-Ag85B IgG showed higher response in non-Beijing TB patients than in Beijing TB patients (p< 0.05; sensitivity, 76.9% versus 44.4%). The sensitivity and specificity analysis showed that 78.8% Beijing infected individuals were negative in anti-TBGL-IgG or/and anti-Ag85B-IgG, while 75.0% of those were positive in anti-TBGL-IgA or/and anti-ACR-IgG tests. These results indicate the possibility of developing antibody-based test to identify Beijing MTB.
Division of Emerging Infectious Diseases, Department of Internal Medicine, Graduate School of Medicine, Tohoku University, Sendai, Miyagi 980-8574, Japan.
Jingge
J
Zhao
Laboratory of Disaster-Related Infectious Disease, International Research Institute of Disaster Science, Tohoku University, Sendai, Miyagi 980-8574, Japan.
Beata
0000-0001-8234-5691
B
Shiratori
Fukujuji Hospital, Japan Anti-Tuberculosis Association, 3-1-2 4 Matsuyama, Kiyose, Tokyo 204-8533, Japan.
Masao
M
Okumura
Fukujuji Hospital, Japan Anti-Tuberculosis Association, 3-1-2 4 Matsuyama, Kiyose, Tokyo 204-8533, Japan.
Hideki
H
Yanai
Microbiological Research Institute, Otsuka Pharmaceutical Co., Ltd., Kawauchi-cho, Tokushima 771-0192, Japan.
Makoto
M
Matsumoto
Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido 001-0020, Japan.
Chie
0000-0002-9194-1062
C
Nakajima
Fukujuji Hospital, Japan Anti-Tuberculosis Association, 3-1-2 4 Matsuyama, Kiyose, Tokyo 204-8533, Japan.
Kazue
K
Mizuno
Microbiological Research Institute, Otsuka Pharmaceutical Co., Ltd., Kawauchi-cho, Tokushima 771-0192, Japan.
Kenji
K
Ono
Microbiological Research Institute, Otsuka Pharmaceutical Co., Ltd., Kawauchi-cho, Tokushima 771-0192, Japan.
Tetsuya
T
Oda
Laboratory of Disaster-Related Infectious Disease, International Research Institute of Disaster Science, Tohoku University, Sendai, Miyagi 980-8574, Japan.
Haorile
0000-0003-0771-6071
H
Chagan-Yasutan
Division of Emerging Infectious Diseases, Department of Internal Medicine, Graduate School of Medicine, Tohoku University, Sendai, Miyagi 980-8574, Japan.
Yugo
Y
Ashino
Division of Bacteriology, Department of Microbiology and Immunology, Faculty of Medicine, Tottori University, Yonago, Tottori 683-8503, Japan.
Takashi
T
Matsuba
Fukujuji Hospital, Japan Anti-Tuberculosis Association, 3-1-2 4 Matsuyama, Kiyose, Tokyo 204-8533, Japan.
Takashi
T
Yoshiyama
Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido 001-0020, Japan.
Yasuhiko
Y
Suzuki
Division of Emerging Infectious Diseases, Department of Internal Medicine, Graduate School of Medicine, Tohoku University, Sendai, Miyagi 980-8574, Japan; Laboratory of Disaster-Related Infectious Disease, International Research Institute of Disaster Science, Tohoku University, Sendai, Miyagi 980-8574, Japan; Graduate School of Health Science Studies, Kibi International University, 8 Igamachi, Takahashi 716-8508, Japan.
Toshio
0000-0003-0947-7625
T
Hattori
D000217:Acyltransferases / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D005260:Female; D005838:Genotype; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D007564:Japan; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology* / Q000302:isolation & purification; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000276:immunology* / Q000382:microbiology; D038201:alpha-Crystallins / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007074:Immunoglobulin G; D038201:alpha-Crystallins; C500452:alpha-crystallin 2, Mycobacterium tuberculosis; C072681:Mycobacterium tuberculosis antigens; D000217:Acyltransferases; C121433:antigen 85B, Mycobacterium tuberculosis