Nile Red Staining of Neutral Lipids in Yeast.
Determination of cellular neutral lipid levels in yeast is important for both the biotechnology industry and biomedical research. However, many of the currently available methods are labor intensive and time consuming. Here we describe a rapid and repeatable method for the detection of neutral lipids, which can be utilized in both oleaginous and non-oleaginous yeast species. The method utilizes the fluorescent dye, Nile red, which enables neutral lipid levels to either be visualized via microscopy or quantified using a 96-well plate assay.
School of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston, Lancashire, UK.|School of Biological Sciences, University of Reading, Reading, Berkshire, UK.
Kerry Ann
KA
Rostron
School of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston, Lancashire, PR1 2HE, UK. cllawrence@uclan.ac.uk.
Clare Louise
CL
Lawrence
D005456:Fluorescent Dyes; D008055:Lipids / Q000737:chemistry; D008856:Microscopy, Fluorescence; D010078:Oxazines; D013194:Staining and Labeling / Q000379:methods; D015003:Yeasts / Q000378:metabolism*
D005456:Fluorescent Dyes; D008055:Lipids; D010078:Oxazines; C044808:nile red
Microplate; Microscopy; Neutral lipids; Nile red; Yeast