In ovo evaluation of FloraMax®-B11 on Marek's disease HVT vaccine protective efficacy, hatchability, microbiota composition, morphometric analysis, and Salmonella enteritidis infection in broiler chickens.
Three experiments were conducted to evaluate the effect of in ovo administration of FloraMax®-B11 (FM) on Marek's disease (MD) herpesvirus of turkeys (HVT) vaccine protective efficacy, hatchability, microbiota composition, morphometric analysis, and Salmonella enteritidis (SE) infection in chickens. Experiment 1 consisted of 3 trials. In trials 1 and 2, d 18 White Leghorn 15I5x71 embryos were randomly distributed in 4 groups: 1) HVT vaccinated in ovo and no Marek's disease virus (MDV) challenge; 2), HVT + FM vaccinated in ovo and no MDV challenge; 3) HVT vaccinated in ovo and challenge with virulent MDV (vMDV; strain 583A); and 4), HVT + FM vaccinated in ovo and challenge with vMDV. Trial 3 was designed exactly the same as Experiment 1 but chicks were challenged with very virulent MDV (vvMDV; strains Md5 and 612). Birds were monitored until 8 wk of age, and tested for MD incidence. Experiment 2 consisted of 3 trials. In each trial, d 18 broiler embryos were injected in ovo with either saline or FM to measure hatchability and gastrointestinal bacterial composition. In Experiment 3, d 18 broiler embryos were injected in ovo with either saline or FM. All chickens that hatched were orally gavaged with SE at hatch and kept for 7 d to monitor post-hatch BW. No significant difference (P > 0.05) between MD percentage in birds vaccinated with HVT alone or HVT + FM were observed in Experiment 1. In Experiment 2, probiotic did not negatively affect hatchability, but did reduce lactose positive Gram-negative bacteria. Further, increase in BW was associated with higher villi surface area in the ileum in chickens that received the probiotic as well as a significant reduction in the SE incidence in Experiment 3. These results suggest that in ovo administration of FM does not negatively impact the ability of HVT to protect against MD or hatchability of chickens, but improves BW during the first 7 d of life and decreases SE recovery in chickens.
Department of Poultry Science, University of Arkansas, Fayetteville 72701.
K D
KD
Teague
Department of Poultry Science, University of Arkansas, Fayetteville 72701.
L E
LE
Graham
USDA, Agricultural Research Service, Avian Disease and Oncology Laboratory, East Lansing, MI 48823.
J R
JR
Dunn
USDA, Agricultural Research Service, Avian Disease and Oncology Laboratory, East Lansing, MI 48823.
H H
HH
Cheng
Department of Poultry Science, University of Arkansas, Fayetteville 72701.
N
N
Anthony
Department of Poultry Science, University of Arkansas, Fayetteville 72701.
J D
JD
Latorre
Pacific Vet Group-USA Inc., 2135 Creek View Drive, Fayetteville, AR 72704.
A
A
Menconi
Pacific Vet Group-USA Inc., 2135 Creek View Drive, Fayetteville, AR 72704.
R E
RE
Wolfenden
Department of Poultry Science, University of Arkansas, Fayetteville 72701.
A D
AD
Wolfenden
Department of Poultry Science, University of Arkansas, Fayetteville 72701.
B D
BD
Mahaffey
Department of Poultry Science, University of Arkansas, Fayetteville 72701.
M
M
Baxter
Department of Veterinary Medicine, National Autonomous University of Mexico, Mexico city 04510.
X
X
Hernandez-Velasco
Department of Veterinary Medicine, National Autonomous University of Mexico, Mexico city 04510.
R
R
Merino-Guzman
Department of Animal Sciences, The Ohio State University, Columbus 44691.
L R
LR
Bielke
Department of Poultry Science, University of Arkansas, Fayetteville 72701.
B M
BM
Hargis
Department of Poultry Science, University of Arkansas, Fayetteville 72701.
G
G
Tellez
D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D026002:Herpesvirus 1, Meleagrid / Q000187:drug effects; D056546:Lactobacillales / Q000737:chemistry*; D008380:Marek Disease / Q000517:prevention & control* / Q000821:virology; D022501:Marek Disease Vaccines / Q000008:administration & dosage / Q000494:pharmacology*; D064307:Microbiota / Q000187:drug effects; D011201:Poultry Diseases / Q000382:microbiology / Q000517:prevention & control* / Q000821:virology; D019936:Probiotics / Q000008:administration & dosage / Q000494:pharmacology*; D012098:Reproduction; D012481:Salmonella Infections, Animal / Q000382:microbiology / Q000517:prevention & control*; D012477:Salmonella enteritidis / Q000187:drug effects
D022501:Marek Disease Vaccines
Marek's disease vaccine; Salmonella enteritidis; hatchability; in ovo; probiotic