Effects of Hypericum perforatum extract on oxaliplatin-induced neurotoxicity: in vitro evaluations.
Hypericum perforatum L. has been used for centuries as a natural remedy for the treatment of many disorders. Neuropathic pain is a common side effect of oxaliplatin-based chemotherapy and often the cause of therapy discontinuation. Thanks to its anti-inflammatory and analgesic effects, the use of H. perforatum may be a novel therapeutic strategy for neuropathy. The aim of this paper was to evaluate the effect of H. perforatum hydrophilic extract on an in vitro model of oxaliplatin-induced neurotoxicity. The antioxidant potential of extract was first evaluated in cell-free models by the thiobarbituric acid-reactive substances assay and nitro blue tetrazolium oxidation test; the ability of H. perforatum extract to reduce oxaliplatin-induced caspase-3 activity in rat astrocytes and its potential interference with the cytotoxic effects of oxaliplatin in a colorectal cancer in vitro model (HT-29 cells) were also evaluated. The extract showed a significant antioxidant effect and was able to reduce caspase-3 activity in rat astrocytes. Of note, the extract alone exerted a cytotoxic effect in HT-29 cells and did not reduce the cytotoxicity of oxaliplatin in HT-29 cells. These data suggest that H. perforatum could be used as a novel therapeutic strategy for counteracting chemotherapy-induced neuropathy.
Department of Neuroscience, Psychology, Drug Research and Child Health - NEUROFARBA - Section of Pharmacology and Toxicology, University of Florence, Viale G. Pieraccini 6, Florence, Italy.
Lorenzo
L
Cinci
Department of Neuroscience, Psychology, Drug Research and Child Health - NEUROFARBA - Section of Pharmacology and Toxicology, University of Florence, Florence, Italy.
Lorenzo
L
Di Cesare Mannelli
Aboca S.p.A. Società Agricola, Località Aboca, San Sepolcro, Arezzo, Italy.
Anna
A
Maidecchi
Aboca S.p.A. Società Agricola, Località Aboca, San Sepolcro, Arezzo, Italy.
Luisa
L
Mattoli
Department of Neuroscience, Psychology, Drug Research and Child Health - NEUROFARBA - Section of Pharmacology and Toxicology, University of Florence, Florence, Italy.
Carla
C
Ghelardini
D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D053148:Caspase 3 / Q000378:metabolism; D019610:Cytoprotection; D019073:HT29 Cells; D006801:Humans; D020902:Hypericum / Q000737:chemistry*; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D020258:Neurotoxicity Syndromes / Q000209:etiology / Q000517:prevention & control*; D009944:Organoplatinum Compounds / Q000633:toxicity*; D000077150:Oxaliplatin; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D051381:Rats
D000970:Antineoplastic Agents; D000975:Antioxidants; D018696:Neuroprotective Agents; D009944:Organoplatinum Compounds; D010936:Plant Extracts; D000077150:Oxaliplatin; C505410:Casp3 protein, rat; D053148:Caspase 3
HT-29; Hypericum perforatum L.; astrocyte; neuropathy; oxaliplatin