Analysis of grain elements and identification of best genotypes for Fe and P in Afghan wheat landraces.
This study was carried out with the aim of developing the methodology to determine elemental composition in wheat and identify the best germplasm for further research. Orphan and genetically diverse Afghan wheat landraces were chosen and EDXRF was used to measure the content of some of the elements to establish elemental composition in grains of 266 landraces using 10 reference lines. Four elements, K, Mg, P, and Fe, were measured by standardizing sample preparation. The results of hierarchical cluster analysis using elemental composition data sets indicated that the Fe content has an opposite pattern to the other elements, especially that of K. By systematic analysis the best wheat germplasms for P content and Fe content were identified. In order to compare the sensitivity of EDXRF, the ICP method was also used and the similar results obtained confirmed the EDXRF methodology. The sampling method for measurement using EDXRF was optimized resulting in high-throughput profiling of elemental composition in wheat grains at low cost. Using this method, we have characterized the Afghan wheat landraces and isolated the best genotypes that have high-elemental content and have the potential to be used in crop improvement.
Department of Biosciences, Kanto Gakuin University College of Science and Engineering, 1-50-1 Mutsura-Higashi, Kanazawa-ku, Yokohama, Kanagawa 236-8501, Japan; Center for Sustainable Resource Science, RIKEN Yokohama Institute, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
Youichi
Y
Kondou
Kihara Institute for Biological Research, Yokohama City University , 641-12 Maioka-cho, Totsuka-ku, Yokohama, Kanagawa 244-0813 , Japan.
Alagu
A
Manickavelu
Kihara Institute for Biological Research, Yokohama City University , 641-12 Maioka-cho, Totsuka-ku, Yokohama, Kanagawa 244-0813 , Japan.
Kenji
K
Komatsu
Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka-cho, Totsuka-ku, Yokohama, Kanagawa 244-0813, Japan; Ministry of Agriculture, Irrigation and Livestock, Afghanistan.
Mujiburahman
M
Arifi
Center for Sustainable Resource Science, RIKEN Yokohama Institute , 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045 , Japan.
Mika
M
Kawashima
Arid Land Research Center, Tottori University , Hamasaka 1390, Tottori 680-0001 , Japan.
Takayoshi
T
Ishii
Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo , 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 , Japan.
Tomohiro
T
Hattori
Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo , 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 , Japan.
Hiroyoshi
H
Iwata
Arid Land Research Center, Tottori University , Hamasaka 1390, Tottori 680-0001 , Japan.
Hisashi
H
Tsujimoto
Kihara Institute for Biological Research, Yokohama City University , 641-12 Maioka-cho, Totsuka-ku, Yokohama, Kanagawa 244-0813 , Japan.
Tomohiro
T
Ban
Center for Sustainable Resource Science, RIKEN Yokohama Institute , 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045 , Japan.
Minami
M
Matsui
best genotypes; elements; high-throughput method; wheat landraces