Nucleic-Acid-Templated Enzyme Cascades.
Cellular metabolism involves complex sequences of organized enzymatic reactions, known as metabolic pathways, that convert substrates into readily usable materials. In nature, these enzymatic complexes are organized in a well-defined manner so that the cascade reactions are more rapid and efficient than they would be if the enzymes were randomly distributed in the cytosol. Development of artificial enzyme cascades that resemble nature's organization of sequentially assembled enzymes is of current interest due to its potential applications, from diagnostics to the production of high-value chemicals. Nucleic acids and their nanostructures have been used to organize enzyme cascades and have been shown to enhance the efficiencies and rates of sequential reactions. Here we summarize the recent progress in the development of artificial enzyme cascades and sequential reactions by arranging enzymes on various DNA/RNA templates and discuss the future directions of this research endeavour.
Institute of Advanced Energy, Kyoto University, Uji, Kyoto, 611-0011, Japan.
Arivazhagan
0000-0002-9319-6107
A
Rajendran
Institute of Advanced Energy, Kyoto University, Uji, Kyoto, 611-0011, Japan.
Eiji
0000-0001-9564-6805
E
Nakata
Institute of Advanced Energy, Kyoto University, Uji, Kyoto, 611-0011, Japan.
Shun
0000-0002-8276-616X
S
Nakano
Institute of Advanced Energy, Kyoto University, Uji, Kyoto, 611-0011, Japan.
Takashi
0000-0003-3663-3267
T
Morii
D004247:DNA / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry*; D009097:Multienzyme Complexes / Q000737:chemistry*; D043942:Nanotubes / Q000737:chemistry; D010316:Particle Size; D012313:RNA / Q000737:chemistry
D004800:Enzymes, Immobilized; D009097:Multienzyme Complexes; D012313:RNA; D004247:DNA
DNA origami; RNA; metabolic pathways; nanodevices; nanostructures; sequential enzymatic reactions