Analysis of Protein O-GlcNAcylation by Mass Spectrometry.
O-linked β-D-N-acetyl glucosamine (O-GlcNAc) addition (O-GlcNAcylation), a post-translational modification of serine/threonine residues of proteins, is involved in diverse cellular metabolic and signaling pathways. Aberrant O-GlcNAcylation underlies the initiation and progression of multiple chronic diseases including diabetes, cancer, and neurodegenerative diseases. Numerous methods have been developed for the analysis of protein O-GlcNAcylation, but instead of discussing the classical biochemical techniques, this unit covers O-GlcNAc characterization by combining several enrichment methods and mass spectrometry detection techniques [including collision-induced dissociation (CID), higher energy collision dissociation (HCD), and electron transfer dissociation (ETD) mass spectrometry]. © 2017 by John Wiley & Sons, Inc.
Department of Biological Chemistry, The Johns Hopkins University, School of Medicine, Baltimore, Maryland.
Junfeng
J
Ma
Department of Biological Chemistry, The Johns Hopkins University, School of Medicine, Baltimore, Maryland.
Gerald W
GW
Hart
D000117:Acetylglucosamine / Q000737:chemistry*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004229:Dithiothreitol / Q000737:chemistry; D006031:Glycosylation; D013058:Mass Spectrometry / Q000379:methods*; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D040901:Proteomics
D011506:Proteins; D004229:Dithiothreitol; D000117:Acetylglucosamine
BEMAD; CID; ETD; HCD; O-GlcNAc; O-GlcNAcome; O-GlcNAcomics; enrichment; mass spectrometry; site mapping