Cofactor recycling for co-production of 1,3-propanediol and glutamate by metabolically engineered Corynebacterium glutamicum.
Production of 1,3-propanediol (1,3-PDO) from glycerol is a promising route toward glycerol biorefinery. However, the yield of 1,3-PDO is limited due to the requirement of NADH regeneration via glycerol oxidation process, which generates large amounts of undesired byproducts. Glutamate fermentation by Corynebacterium glutamicum is an important oxidation process generating excess NADH. In this study, we proposed a novel strategy to couple the process of 1,3-PDO synthesis with glutamate production for cofactor regeneration. With the optimization of 1,3-PDO synthesis route, C. glutamicum can efficiently convert glycerol into 1,3-PDO with the yield of ~ 1.0 mol/mol glycerol. Co-production of 1,3-PDO and glutamate was also achieved which increased the yield of glutamate by 18% as compared to the control. Since 1,3-PDO and glutamate can be easily separated in downstream process, this study provides a potential green route for coupled production of 1,3-PDO and glutamate to enhance the economic viability of biorefinery process.
Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
Jinhai
J
Huang
Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
Yao
Y
Wu
Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
Wenjun
W
Wu
Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
Ye
Y
Zhang
Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.|Tsinghua Innovation Center in Dongguan, Dongguan 523808, China.
Dehua
D
Liu
Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.|Tsinghua Innovation Center in Dongguan, Dongguan 523808, China.
Zhen
Z
Chen
D000332:Aerobiosis / Q000187:drug effects; D053898:Biosynthetic Pathways / Q000187:drug effects; D003067:Coenzymes / Q000378:metabolism*; D048230:Corynebacterium glutamicum / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism*; D005990:Glycerol / Q000494:pharmacology; D006836:Hydro-Lyases / Q000378:metabolism; D060847:Metabolic Engineering / Q000379:methods*; D009243:NAD / Q000378:metabolism; D011409:Propylene Glycols / Q000378:metabolism*; D013379:Substrate Specificity / Q000187:drug effects
D003067:Coenzymes; D011409:Propylene Glycols; D009243:NAD; D018698:Glutamic Acid; C041787:1,3-propanediol; D006836:Hydro-Lyases; C020671:glycerol dehydratase; D005990:Glycerol