Prevention of "nitrosative stress" by a nutritional supplement (LaVita®) - a randomized placebo controlled double blind clinical trial with healthy volunteers.
BACKGROUND
A common pathomechanism involved in many degenerative manifestations of non-communicable diseases is nitrosative stress, giving rise to a chronic insidious inflammation causing silent inflammation at a cellular level. The release of nitric oxide inhibits multiple enzyme reactions with reduced oxidative phosphorylation and mitochondrial ATP depletion.


METHODS
We hypothesized that enzyme-inhibition can be alleviated by micronutrient supply, and studied laboratory parameters associated with nitrosative stress (nitrotyrosine, mitochondrial activity) after a micronutrient supplementation (a multivitamin mineral and trace element formulation as verum: LaVita®) and a placebo in healthy volunteers (n=150) for six months.


RESULTS
Mean nitrotyrosine levels dropped significantly after 3 month in the verum and placebo group, whereas mitochondrial activity increased after three month in the verum group (p=0,087), but not in the placebo group (p=0,990). Ubiquinone - an essential ingredient for mitochondrial function- increased after six months in the verum group, but not after placebo consumption (p=0,001). Serum zinc and cellular zinc increased steadily after 3 and 6 month verum intake (p<0,001). As the enzyme superoxide dismutase (SOD) is mainly involved in the formation of nitrosative stress (peroxides) we measured the activity, and found significant differences in the placebo and verum group after 3 and 6 month (p=0,050 and p=0,003 respectively).


CONCLUSIONS
We conclude that a balanced combination of vital nutrients may reduce nitrosative stress and silent inflammation, and consequently the risk for various forms of degenerative diseases.
Internationale Gesellschaft für angewandte Präventionsmedizin I-GAP, Währingerstrasse 63, A-1090 Wien. Privates Institut für klinische Ernärungs- und Präventionsmedizin PIKEM; Associated Professor Public Health St. Elisabeth Unive, Austria.
Claus
C
Muss
Inst. f. Krebsforschung, KIM-1, Medizinische Universität, Wien, Austria.
Wilhelm
W
Mosgoeller
Labor Endler, Währingerstrasse 63, Wien, Austria.
Thomas
T
Endler
D000328:Adult; D019587:Dietary Supplements; D004311:Double-Blind Method; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D007249:Inflammation / Q000517:prevention & control; D008297:Male; D014131:Trace Elements / Q000008:administration & dosage* / Q000494:pharmacology; D014451:Ubiquinone / Q000097:blood; D014815:Vitamins / Q000008:administration & dosage / Q000494:pharmacology*; D015032:Zinc / Q000097:blood
D014131:Trace Elements; D014815:Vitamins; D014451:Ubiquinone; D015032:Zinc