Evaluation of recombinant protein superoxide dismutase of Haemophilus parasuis strain SH0165 as vaccine candidate in a mouse model.
Haemophilus parasuis can cause a severe membrane inflammation disorder. It has been documented that superoxide dismutase (SOD) is a potential target to treat systemic inflammatory diseases. Therefore, we constructed an experimental H. parasuis subunit vaccine SOD and determined the protective efficacy of SOD using a lethal dose challenge against H. parasuis serovar 4 strain MD0322 and serovar 5 strain SH0165 in a mouse model. The results demonstrated that SOD could induce a strong humoral immune response in mice and provide significant immunoprotection efficacy against a lethal dose of H. parasuis serovar 4 strain MD0322 or serovar 5 strain SH0165 challenge. IgG subtype analysis indicated SOD protein could trigger a bias toward a Th1-type immune response and induce the proliferation of splenocytes and secretion of IL-2 and IFN-γ of splenocytes. In addition, serum in mice from the SOD-immunized group could inhibit the growth of strain MD0322 and strain SH0165 in the whole-blood killing bacteria assay. This is the first report that immunization of mice with SOD protein could provide protective effect against a lethal dose of H. parasuis serovar 4 and serovar 5 challenge in mice, which may provide a novel approach against heterogeneous serovar infection of H. parasuis in future.
a Hubei Key Laboratory of Animal Nutrition and Feed Science, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan 430023, People's Republic of China.
Ling
L
Guo
a Hubei Key Laboratory of Animal Nutrition and Feed Science, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan 430023, People's Republic of China.
Lei
L
Xu
a Hubei Key Laboratory of Animal Nutrition and Feed Science, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan 430023, People's Republic of China.
Tao
T
Wu
a Hubei Key Laboratory of Animal Nutrition and Feed Science, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan 430023, People's Republic of China.
Shulin
S
Fu
a Hubei Key Laboratory of Animal Nutrition and Feed Science, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan 430023, People's Republic of China.
Yinsheng
Y
Qiu
a Hubei Key Laboratory of Animal Nutrition and Feed Science, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan 430023, People's Republic of China.|b Biochemistry and Molecular Biology, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA.
Chien-An Andy
CA
Hu
c College of Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
Xinglong
X
Ren
d School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, People's Republic of China.
Rongrong
R
Liu
d School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, People's Republic of China.
Mengdie
M
Ye
D000818:Animals; D016207:Cytokines / Q000096:biosynthesis; D004195:Disease Models, Animal; D005260:Female; D018073:Haemophilus Vaccines / Q000276:immunology*; D044137:Haemophilus parasuis / Q000201:enzymology / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D013482:Superoxide Dismutase / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology
D016207:Cytokines; D018073:Haemophilus Vaccines; D014614:Vaccines, Synthetic; D013482:Superoxide Dismutase
Glässer’s disease; Haemophilus parasuis; maladie de Glässer; superoxide dismutase; superoxyde dismutase; vaccin; vaccine