Stress mediated changes in expression of the pkaC gene, encoding the catalytic subunit of cAMP-dependent protein kinase, in Aspergillus niger.
The transcriptional regulation of the pkaC gene, encoding the catalytic subunit of cAMP-dependent protein kinase from Aspergillus niger, was analysed under different environmental conditions. Quantitative determination of pkaC transcript showed a significant decrease in concentration of specific mRNA immediately after a temperature, hypoosmotic and hyperosmotic shock followed by stimulated synthesis. The amount of pkaC mRNA as well as PKA enzymatic activity steadily decreased during the initial phase of growth in 15 % sucrose medium while a slight increase was observed at the time of a change in morphology from bulbous cells to filamentous growth. Transcriptional alternation might be mediated by multiple putative stress elements in the promoter region of pkaC gene.
Department of Biotechnology and Industrial Mycology, National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia, , , , , , SI.
Mojca
M
Benčina
Department of Biotechnology and Industrial Mycology, National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia, , , , , , SI.
Matic
M
Legiša
Aspergillus niger; Key words, cAMP-dependent protein kinase; reverse transcription-PCR; stress response