Adiponectin promotes human jaw bone marrow mesenchymal stem cell chemotaxis via CXCL1 and CXCL8.
Adiponectin (APN) is known to promote the osteogenic differentiation of human jaw bone marrow mesenchymal stem cells (h-JBMMSCs). However, the underlying mechanism has not been fully elucidated. Previously, we showed that APN could promote h-JBMMSC osteogenesis via APPL1-p38 by up-regulating osteogenesis-related genes. Here, we aimed to determine whether APN could promote h-JBMMSC chemotaxis through CXCL1/CXCL8. The CCK-8, wound healing and transwell assays were used to evaluate the proliferation, migration and chemotaxis of h-JBMMSCs with or without APN treatment. Chemotaxis-related genes were screened using RNA-seq, and the results were validated using real-time PCR and ELISA. We also performed Western blot using the AMPK inhibitor, WZ4003, and the p38 MAPK inhibitor, SB203580, to identify the signalling pathway involved. We found that APN could promote h-JBMMSC chemotaxis in the co-culture transwell system. CXCL1 and CXCL8 were screened and confirmed as the up-regulated target genes. The APN-induced CXCL1/8 up-regulation to promote chemotaxis could be blocked by CXCR2 inhibitor SB225002. Western blot revealed that the phosphorylation of AMPK and p38 MAPK increased in a time-dependent manner with APN treatment. Additionally, WZ4003 and SB203580 could suppress the APN-induced overexpression of CXCL1 and CXCL8. The results of the transwell chemotaxis assay also supported the above results. Our data suggest that APN can promote h-JBMMSC chemotaxis by up-regulating CXCL1 and CXCL8.
2nd Dental Center, Peking University School and Hospital of Stomatology, Beijing, China.|Central Laboratory, Peking University School and Hospital of Stomatology, Beijing, China.
Yinfei
0000-0002-5764-5438
Y
Pu
2nd Dental Center, Peking University School and Hospital of Stomatology, Beijing, China.|Central Laboratory, Peking University School and Hospital of Stomatology, Beijing, China.
Mengke
M
Wang
Central Laboratory, Peking University School and Hospital of Stomatology, Beijing, China.|Department of Pathology, Peking University School and Hospital of Stomatology, Beijing, China.
Yingying
Y
Hong
2nd Dental Center, Peking University School and Hospital of Stomatology, Beijing, China.|National Engineering Laboratory for Digital and Material Technology of Stomatology, Peking University School and Hospital of Stomatology, Beijing, China.
Yuwei
Y
Wu
2nd Dental Center, Peking University School and Hospital of Stomatology, Beijing, China.|National Engineering Laboratory for Digital and Material Technology of Stomatology, Peking University School and Hospital of Stomatology, Beijing, China.
Zhihui
Z
Tang
D052242:Adiponectin / Q000008:administration & dosage* / Q000378:metabolism; D000813:Anilides / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D054360:Chemokine CXCL1 / Q000037:antagonists & inhibitors / Q000235:genetics*; D002633:Chemotaxis / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006801:Humans; D016209:Interleukin-8 / Q000037:antagonists & inhibitors / Q000235:genetics*; D008445:Maxillofacial Development / Q000187:drug effects / Q000235:genetics*; D059630:Mesenchymal Stem Cells / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects / Q000235:genetics*; D010671:Phenylurea Compounds / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics
D052242:Adiponectin; D000813:Anilides; C475829:CXCL1 protein, human; C508606:CXCL8 protein, human; D054360:Chemokine CXCL1; D016209:Interleukin-8; D010671:Phenylurea Compounds; D011743:Pyrimidines; C112019:SB 225002; C586755:WZ4003; D048051:p38 Mitogen-Activated Protein Kinases
CXCL1; CXCL8; adiponectin; chemotaxis; jaw bone; stem cells