A homolog of cyclophilin D is expressed inTrypanosoma cruziand is involved in the oxidative stress-damage response.
Mitochondria have an important role in energy production, homeostasis and cell death. The opening of the mitochondrial permeability transition pore (mPTP) is considered one of the key events in apoptosis and necrosis, modulated by cyclophilin D (CyPD), a crucial component of this protein complex. InTrypanosoma cruzi, the protozoan parasite that causes Chagas disease, we have previously described that mitochondrial permeability transition occurs after oxidative stress induction in a cyclosporin A-dependent manner, a well-known cyclophilin inhibitor. In the present work, a mitochondrial parasite cyclophilin, namedTcCyP22, which is homolog to the mammalian CyPD was identified.TcCyP22-overexpressing parasites showed an enhanced loss of mitochondrial membrane potential and loss of cell viability when exposed to a hydrogen peroxide stimulus compared with control parasites. Our results describe for the first time in a protozoan parasite that a mitochondrial cyclophilin is a component of the permeability transition pore and is involved in regulated cell death induced by oxidative stress.
Instituto Nacional de Parasitología 'Dr. Mario Fatala Chabén'- A.N.L.I.S. Malbrán, Av. Paseo Colón 568, C1063AC S, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina.
Patricia L
PL
Bustos
Instituto Nacional de Parasitología 'Dr. Mario Fatala Chabén'- A.N.L.I.S. Malbrán , Av. Paseo Colón 568, C1063AC S, Buenos Aires, Argentina.
Bibiana J
BJ
Volta
Instituto Nacional de Parasitología 'Dr. Mario Fatala Chabén'- A.N.L.I.S. Malbrán , Av. Paseo Colón 568, C1063AC S, Buenos Aires, Argentina.
Alina E
AE
Perrone
Instituto Nacional de Parasitología 'Dr. Mario Fatala Chabén'- A.N.L.I.S. Malbrán, Av. Paseo Colón 568, C1063AC S, Buenos Aires, Argentina; CAECIHS, Universidad Abierta Interamericana, Av. Montes de Oca 745, 2º piso, C1270AAH, Buenos Aires, Argentina.
Natalia
N
Milduberger
Instituto Nacional de Parasitología 'Dr. Mario Fatala Chabén'- A.N.L.I.S. Malbrán, Av. Paseo Colón 568, C1063AC S, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina; CAECIHS, Universidad Abierta Interamericana, Av. Montes de Oca 745, 2º piso, C1270AAH, Buenos Aires, Argentina.
Jacqueline
J
Bua