A visible and colorimetric aptasensor based on DNA-capped single-walled carbon nanotubes for detection of exosomes.
Recently, many studies have shown the potential use of circulating exosomes as novel biomarkers for monitoring and predicting a number of complex diseases, including cancer. However, reliable and cost-effective detection of exosomes in routine clinical settings, still remain a difficult task, mainly due to the lack of adequately easy and fast assay platforms. Therefore, we demonstrate here the development of a visible and simple method for the detection of exosomes by integrating single-walled carbon nanotubes that being excellent water solubility (s-SWCNTs) and aptamer. Aptamers, specific to exosomes transmembrane protein CD63, are absorbed onto the surface of s-SWCNTs and improve the minic peroxidase activity of s-SWCNTs, which can efficiently catalyze H2O2-mediated oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) and lead to a change from colorless to blue in solution. However, after adding exosomes, the aptamers are bound with CD63, leaving from the surface of s-SWCNTs through conformational changes, which results the color of solution from deep to moderate, and this can be observed by the naked eye and monitored by UV-vis spectrometry. Under optimal conditions, the linear range of exosomes is estimated to be 1.84×106to 2.21×107particles/μL with a detection of limit (LOD) of 5.2×105particles/μL. Consequently, a visible and simple approach detecting exosomes is successfully constructed. Moreover, this proposed colorimetric aptasensor can be universally applicable for the detection of other targets by simple change the aptamer.
Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China.
Yaokun
Y
Xia
Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China.
Mengmeng
M
Liu
Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China.
Liangliang
L
Wang
Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China.
An
A
Yan
Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China.
Wenhui
W
He
Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China.
Mei
M
Chen
Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China.
Jianming
J
Lan
Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian Province 350108, PR China; Fujian Provincial Key Laboratory of Nanomaterials, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian Province 350108, PR China.
Jiaoxing
J
Xu
Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian Province 350108, PR China; Fujian Provincial Key Laboratory of Nanomaterials, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian Province 350108, PR China. Electronic address: guanlh@fjirsm.ac.cn.
Lunhui
L
Guan
Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China. Electronic address: cjh_huaxue@126.com.
Jinghua
J
Chen
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001560:Benzidines / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D002863:Chromogenic Compounds / Q000737:chemistry; D003124:Colorimetry / Q000379:methods*; D055354:Exosomes / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D061986:MCF-7 Cells; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000737:chemistry; D060149:Tetraspanin 30 / Q000032:analysis*
D052157:Aptamers, Nucleotide; D001560:Benzidines; C554586:CD63 protein, human; D002863:Chromogenic Compounds; D037742:Nanotubes, Carbon; D060149:Tetraspanin 30; C021758:3,3',5,5'-tetramethylbenzidine; D006861:Hydrogen Peroxide; D009195:Peroxidase
DNA-capped SWCNTs; Exosomes; Visible and colorimetric aptasensor