α-Synuclein binds to cytoplasmic vesicles in U251 glioblastoma cells.
α-Synuclein is the major component of Lewy bodies, Lewy neurites, and glial cytoplasmic inclusions. It plays an important role in neurodegenerative diseases such as Parkinson's disease, multiple system atrophy, and other synucleinopathies. However, the pathogenesis and neurodegenerative effects of α-synuclein remain unknown. In this study, we established an α-synuclein and an α-synuclein-EGFP overexpressing U251 cell line. α-Synuclein overexpression increases oxidative stress and alters the cell surface and mitochondrial morphologies. We provide fluorescent-protein tagging, immunofluorescence and ultrastructural evidence showing that α-synuclein accumulations are associated with clusters of cytoplasmic vesicles and the diameter of these vesicles increases by H2O2in a time- and dose-dependent manner.
Department of Clinical Laboratory Medicine, Chinese PLA General Hospital, PRChina; School of Life Science, Beijing Institute of Technology, PRChina.
Jinyan
J
Duan
School of Life Science, Beijing Institute of Technology, PRChina.
Zhengxin
Z
Ying
School of Life Science, Beijing Institute of Technology, PRChina.
Yang
Y
Su
School of Life Science, Beijing Institute of Technology, PRChina.
Fankai
F
Lin
School of Life Science, Beijing Institute of Technology, PRChina. Electronic address: Deng@bit.edu.cn.
Yulin
Y
Deng
D001932:Brain Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D022162:Cytoplasmic Vesicles / Q000378:metabolism*; D005909:Glioblastoma / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology; D011485:Protein Binding; D051844:alpha-Synuclein / Q000378:metabolism*
D051844:alpha-Synuclein; D008315:Malondialdehyde; D006861:Hydrogen Peroxide
Cytoplasmic vesicles; Oxidative stress; α-synuclein