Evaluation of drug loading capabilities of γ-cyclodextrin-metal organic frameworks by high performance liquid chromatography.
Drug loading into γ-cyclodextrin-metal organic frameworks (γ-CD-MOFs) using the impregnation approach is a laborious process. In this study, a γ-CD-MOF construct (2-5μm particle diameter) was used as the stationary phase under HPLC conditions with the aim to correlate retention properties and drug loading capability of the CD-based structure. Ketoprofen, fenbufen and diazepam were chosen as model drugs with m-xylene as a control analyte to investigate the correlation of drug loading and their chromatographic behaviour in the γ-CD-MOF column. Furthermore, γ-CD itself was also prepared as the stationary phase by coupling with silica in the column to illustrate the enhanced interaction between drugs and γ-CD-MOF as a reference. The retention and loading efficiency of the drugs were determined with different ratios of hexane and ethanol (10:90, 20:80, 50:50, 80:20, 90:10, v/v) at temperatures of 20, 25, 30 and 37°C. With the increment in hexane content, the loading efficiency of ketoprofen and fenbufen increased from 2.39±0.06% to 4.38±0.04% and from 5.82±0.94% to 6.37±0.29%, respectively. The retention time and loading efficiency of ketoprofen and diazepam were the lowest at 30°C while those of fenbufen had the different tendency. The excellent relation between the retention and loading efficiency onto γ-CD-MOF could be clearly observed through mobile phase and temperature investigation. In conclusion, a highly efficient chromatographic method has been established to evaluate the drug loading capability of γ-CD-MOF.
Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China; Center for Drug Delivery Systems, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Xiaonan
X
Xu
Center for Drug Delivery Systems, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Caifen
C
Wang
Center for Drug Delivery Systems, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Haiyan
H
Li
Center for Drug Delivery Systems, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; Institut de Sciences Moléculaires d'Orsay, Université Paris-Saclay, UMR CNRS 8214, 91400 Orsay Cedex, France.
Xue
X
Li
Center for Drug Delivery Systems, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Botao
B
Liu
Center for Drug Delivery Systems, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Vikramjeet
V
Singh
Center for Drug Delivery Systems, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Shuxia
S
Wang
Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China. Electronic address: slx04@163.com.
Lixin
L
Sun
Institut de Sciences Moléculaires d'Orsay, Université Paris-Saclay, UMR CNRS 8214, 91400 Orsay Cedex, France. Electronic address: ruxandra.gref@u-psud.fr.
Ruxandra
R
Gref
Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China; Center for Drug Delivery Systems, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. Electronic address: jwzhang@simm.ac.cn.
Jiwen
J
Zhang
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D021621:Imaging, Three-Dimensional; D008670:Metals / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D009930:Organic Chemicals / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry; D011208:Powders; D013696:Temperature; D014961:X-Ray Diffraction; D047408:gamma-Cyclodextrins / Q000737:chemistry*
D008670:Metals; D009930:Organic Chemicals; D004364:Pharmaceutical Preparations; D011208:Powders; D047408:gamma-Cyclodextrins; C023792:gamma-cyclodextrin
Drug loading efficiency; Metal-organic frameworks; Retention; γ-CD-MOFs