Characteristics of expression and regulation of sirtuins in chicken (Gallus gallus).
Sirtuins (SIRT1-SIRT7) are a family of NAD+-dependent protein deacetylases that are linked to post-translational regulation of many metabolic processes. There are few reports available for chicken sirtuins (designated cSIRT1-cSIRT7), whose expression and regulation in the liver have yet to be explored. In the present study, we characterized the expression and regulation of sirtuin family members in chicken liver. The results showed that the sirtuin family members in chicken share the same conserved functional SIR2 domains. All the sirtuin family members were expressed extensively in all tissues examined, and the expression levels of cSIRT1, cSIRT2, cSIRT4, cSIRT6, and cSIRT7 in the liver increased significantly with sexual maturity. However, all sirtuin family members were downregulated (P < 0.05) in chicken livers and cultured primary hepatocytes treated with 17β-estradiol. We concluded that the expression levels of some chicken sirtuin family members in the liver were upregulated with sexual maturation, but might not be regulated directly by estrogen. Whereas estrogen could be used as an inhibitor of all sirtuins, both in vivo and in vitro.
a College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
Junxiao
J
Ren
a College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
Naiyi
N
Xu
a College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
Zheng
Z
Ma
a College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
Yanmin
Y
Li
a College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.|b Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou 450002, China.|c International Joint Research Laboratory for Poultry Breeding of Henan, Henan, Zhengzhou 450002, China.
Cuicui
C
Li
a College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.|b Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou 450002, China.|c International Joint Research Laboratory for Poultry Breeding of Henan, Henan, Zhengzhou 450002, China.
Yanbin
Y
Wang
a College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.|b Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou 450002, China.|c International Joint Research Laboratory for Poultry Breeding of Henan, Henan, Zhengzhou 450002, China.
Yadong
Y
Tian
a College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.|b Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou 450002, China.|c International Joint Research Laboratory for Poultry Breeding of Henan, Henan, Zhengzhou 450002, China.
Xiaojun
X
Liu
a College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.|b Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou 450002, China.|c International Joint Research Laboratory for Poultry Breeding of Henan, Henan, Zhengzhou 450002, China.
Xiangtao
X
Kang
D000818:Animals; D030161:Avian Proteins / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D002645:Chickens / Q000235:genetics*; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005810:Multigene Family; D012741:Sexual Maturation / Q000235:genetics; D056564:Sirtuin 1 / Q000235:genetics / Q000378:metabolism; D056565:Sirtuin 2 / Q000235:genetics / Q000378:metabolism; D037761:Sirtuins / Q000235:genetics* / Q000378:metabolism
D030161:Avian Proteins; D004967:Estrogens; D004958:Estradiol; D056564:Sirtuin 1; D056565:Sirtuin 2; D037761:Sirtuins
chicken; estrogen; estrogène; foie; liver; poulet; sirtuin; sirtuine