Low-intensity focused ultrasound (LIFU)-induced acoustic droplet vaporization in phase-transition perfluoropentane nanodroplets modified by folate for ultrasound molecular imaging.
The commonly used ultrasound (US) molecular probes, such as targeted microbubbles and perfluorocarbon emulsions, present a number of inherent problems including the conflict between US visualization and particle penetration. This study describes the successful fabrication of phase changeable folate-targeted perfluoropentane nanodroplets (termed FA-NDs), a novel US molecular probe for tumor molecular imaging with US. Notably, these FA-NDs can be triggered by low-intensity focused US (LIFU) sonication, providing excellent US enhancement in B-mode and contrast-enhanced US mode in vitro. After intravenous administration into nude mice bearing SKOV3 ovarian carcinomas, 1,1'-dioctadecyl-3,3,3',3' -tetramethylindotricarbocya-nine iodide-labeled FA-NDs were found to accumulate in the tumor region. FA-NDs injection followed by LIFU sonication exhibited remarkable US contrast enhancement in the tumor region. In conclusion, combining our elaborately developed FA-NDs with LIFU sonication provides a potential protocol for US molecular imaging in folate receptor-overexpressing tumors.
Chongqing Key Laboratory of Ultrasound Molecular Imaging.
Jianxin
J
Liu
Chongqing Key Laboratory of Ultrasound Molecular Imaging.
Tingting
T
Shang
Chongqing Key Laboratory of Ultrasound Molecular Imaging.
Fengjuan
F
Wang
Chongqing Key Laboratory of Ultrasound Molecular Imaging.
Yang
Y
Cao
Chongqing Key Laboratory of Ultrasound Molecular Imaging.
Lan
L
Hao
Chongqing Key Laboratory of Ultrasound Molecular Imaging; Department of Ultrasound.
JianLi
J
Ren
Chongqing Key Laboratory of Ultrasound Molecular Imaging; Department of Ultrasound.
Haitao
H
Ran
Chongqing Key Laboratory of Ultrasound Molecular Imaging; Department of Ultrasound.
Zhigang
Z
Wang
Chongqing Key Laboratory of Ultrasound Molecular Imaging; Department of Ultrasound.
Pan
P
Li
Postgraduate Department, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.
Zhiyu
Z
Du
D000818:Animals; D003287:Contrast Media / Q000008:administration & dosage; D005260:Female; D005466:Fluorocarbons / Q000737:chemistry*; D005492:Folic Acid / Q000737:chemistry*; D006979:Hyperthermia, Induced; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D045423:Microbubbles; D057054:Molecular Imaging / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*; D010051:Ovarian Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology; D044367:Phase Transition; D013010:Sonication; D014465:Ultrasonics; D014835:Volatilization; D023041:Xenograft Model Antitumor Assays; D010420:Pentanes
D003287:Contrast Media; D005466:Fluorocarbons; D005492:Folic Acid; C008806:perfluoropentane; D010420:Pentanes
acoustic droplet vaporization; low-intensity focused ultrasound; perfluoropentane nanodroplets; targeting; ultrasound molecular imaging