Effects of neonatal 17α-ethinyloestradiol exposure on female-paced mating behaviour in the rat.
Correct perinatal oestrogen levels are critical for sexual differentiation. For example, perinatal exposure to oestrogen causes masculinization and defeminization of the brain in female rats and also induces delayed effects after maturation characterized by early onset of abnormal oestrus cycling. However, the mechanisms underlying the above effects of oestrogen remain to be fully determined. 17α-ethinyloestradiol (EE), a common synthetic oestrogen widely used in oral contraceptives, binds specifically to oestrogen receptors. In this study, we demonstrated the effects of a single neonatal injection of high- or low-dose EE on reproductive behaviours. Female rats within 24 h after birth were subcutaneously injected with sesame oil, EE (0.02, 2 mg kg-1) and 17β-oestradiol (E2) (20 mg kg-1). Between 11 and 15 weeks of age, sexual behaviour was tested twice in a paced mating situation. Latency to enter, lordosis and soliciting behaviour were recorded. Both high-dose EE- and E2-treated females showed a significantly lower lordosis quotient, decreased soliciting behaviours, increased rejection and fighting numbers. Accessibility to males was also delayed by neonatal E2exposure, although it was shortened by high-dose EE exposure. In contrast, low-dose EE-treated females did not exhibit impaired sexual behaviour. These results suggest that single neonatal exposure to a high dose of EE or E2disturbs the normal development of the female brain, resulting in impaired sexual behaviours in a female-paced mating situation. Besides, the differences noted between high-dose EE- and E2-treated females might be caused by different affinities of the oestrogen receptors, metabolic rates or mechanisms of action. Copyright © 2017 John Wiley & Sons, Ltd.
Laboratory of Animal Behavior and Environmental Science, School of Agriculture, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki, Kanagawa, 214-8571, Japan.
Chiaki
C
Komine
Laboratory of Animal Behavior and Environmental Science, School of Agriculture, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki, Kanagawa, 214-8571, Japan.
Shingo
S
Nakajima
Department of Animal Sciences, Teikyo University of Science, 2-2-1 Senju-Sakuragi, Adachi-ku, Tokyo, Japan.
Yasuhiko
Y
Kondo
Laboratory of Animal Behavior and Environmental Science, School of Agriculture, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki, Kanagawa, 214-8571, Japan.
Yasuyuki
Y
Horii
National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, Japan.
Midori
M
Yoshida
Laboratory of Animal Behavior and Environmental Science, School of Agriculture, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki, Kanagawa, 214-8571, Japan.
Maiko
0000-0002-3184-9483
M
Kawaguchi
D000818:Animals; D000831:Animals, Newborn; D003276:Contraceptives, Oral; D004305:Dose-Response Relationship, Drug; D004967:Estrogens / Q000008:administration & dosage / Q000633:toxicity*; D004997:Ethinyl Estradiol / Q000008:administration & dosage / Q000633:toxicity*; D005260:Female; D007279:Injections, Subcutaneous; D008297:Male; D017208:Rats, Wistar; D012726:Sexual Behavior, Animal / Q000187:drug effects*
D003276:Contraceptives, Oral; D004967:Estrogens; D004997:Ethinyl Estradiol
17α-ethinyloestradiol; female rats; lordosis reflection; neonatal single exposure; paced mating; sexual behaviour