Micro-RNA detection based on fluorescence resonance energy transfer of DNA-carbon quantum dots probes.
Carbon quantum dots have been proposed as an effective platform for miRNA detection. Carbon dots were synthesized by citric acid. The synthesized dots were characterized by dynamic light scattering, UV-Vis spectrophotometry, spectrofluorimetry, transmission electron microscopy and FT-IR spectrophotometry. The fluorescence quantum yield of the synthesized dots was determined using quinine sulfate as the standard. The FAM-labeled single stranded DNA, as sensing element, was adsorbed on dots by π-π interaction. The quenching of the dots fluorescence due to fluorescence resonance energy transfer (FRET) was used for mir 9-1 detection. In the presence of the complementary miRNA, the FRET did not take place and the fluorescence was recovered.
Department of Chemistry and Nanochemistry, Faculty of Sciences and Modern Technologies, Graduate University of Advanced Technology, Kerman, Iran.
Faeze
F
Khakbaz
Department of Chemistry and Nanochemistry, Faculty of Sciences and Modern Technologies, Graduate University of Advanced Technology, Kerman, Iran. Electronic address: mahani@kgut.ac.ir.
Mohamad
M
Mahani
D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry*; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D045663:Quantum Dots; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
D015342:DNA Probes; D004277:DNA, Single-Stranded; C541874:MIRN92 microRNA, human; D035683:MicroRNAs; D002244:Carbon
Carbon quantum dots; FAM; FRET; Mir 9-1; Quantum yield; Single stranded DNA