Comparison of immunochromatographic assays based on fluorescent microsphere and quantum-dot submicrobead for quantitative detection of aflatoxin M1in milk.
The performance of fluorescent microsphere immunochromatographic assay (FM-ICA) and quantum-dot submicrobead immunochromatographic assay (QB-ICA) was systematically and comprehensively compared in quantitative detection of aflatoxin M1in milk. Under optimum conditions, the advantages of FM-ICA include lower limit of detection of 42.3 pg/mL with better accuracy, precision, reliability, and practicability. The advantages of QB-ICA include shorter detection time and lower monoclonal antibody consumption. The 2 ICA were consistent with liquid chromatography-tandem mass spectrometry. This study serves as a reference for selecting appropriate fluorescent labels for the immunochromatographic assay of aflatoxin M1.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Chenghui
C
Wu
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Liming
L
Hu
Jiangxi Institute of Veterinary Drug and Feedstuff Control, Nanchang 330047, China.
Jun
J
Xia
Jiangxi Institute of Veterinary Drug and Feedstuff Control, Nanchang 330047, China.
Guomao
G
Xu
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Kai
K
Luo
Jiangxi Province Center for Disease Control and Prevention, Nanchang 330047, China.
Daofeng
D
Liu
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Hong
H
Duan
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Song
S
Cheng
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Yonghua
Y
Xiong
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address: talktolaiwh@163.com.
Weihua
W
Lai
D016607:Aflatoxin M1 / Q000032:analysis; D000818:Animals; D002846:Chromatography, Affinity; D008863:Microspheres; D008892:Milk / Q000737:chemistry; D015203:Reproducibility of Results
D016607:Aflatoxin M1
fluorescent microsphere; immunochromatographic assay; milk; quantum-dot submicrobead