Evidence for disulfide bonds in SR Protein Kinase 1 (SRPK1) that are required for activity and nuclear localization.
Serine/arginine protein kinases (SRPKs) phosphorylate Arg/Ser dipeptide-containing proteins that play crucial roles in a broad spectrum of basic cellular processes. The existence of a large internal spacer sequence that separates the bipartite kinase catalytic core is a unique structural feature of SRPKs. Previous structural studies on a catalytically active fragment of SRPK1, which lacks the main part of the spacer domain, revealed that SRPK1 remains in an active state without any post-translational modifications or specific intra-protein interactions, while the spacer domain is depicted as a loop structure, outside the kinase core. Using systematic mutagenesis we now provide evidence that replacement of any individual cysteine residue in the spacer, apart from Cys414, or in its proximal flaking ends of the two kinase catalytic domains has an impact on kinase activity. Furthermore, the cysteine residues are critical for nuclear translocation of SRPK1 in response to genotoxic stress and SRPK1-dependent splicing of a reporter gene. While replacement of Cys207, Cys502 and Cys539 of the catalytic domains is predicted to distort the kinase active structure, our findings suggest that Cys356, Cys386, Cys427 and Cys455 of the spacer domain and Cys188 of the first catalytic domain are engaged in disulfide bridging. We propose that such a network of intramolecular disulfide bonds mediates the bending of the spacer region thus allowing the proximal positioning of the two catalytic subunits which is a prerequisite for SRPK1 activity.
Laboratory of Biochemistry, Department of Chemistry, Aristotle University, Thessaloniki, Greece.
Maria
M
Koutroumani
Department of Biochemistry and Biotechnology, University of Thessaly, Mezourlo, Larisa, Greece.
Georgios E
GE
Papadopoulos
Institute of Biosciences & Applications, National Centre for Scientific Research "Demokritos", Athens, Greece.
Metaxia
M
Vlassi
Laboratory of Biochemistry, Department of Chemistry, Aristotle University, Thessaloniki, Greece.
Eleni
E
Nikolakaki
Laboratory of Biochemistry, Department of Chemistry, Aristotle University, Thessaloniki, Greece.
Thomas
T
Giannakouros
D000595:Amino Acid Sequence; D002460:Cell Line; D002467:Cell Nucleus; D004220:Disulfides / Q000737:chemistry; D004789:Enzyme Activation; D015870:Gene Expression; D017930:Genes, Reporter; D006801:Humans; D008958:Models, Molecular; D009154:Mutation; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D012326:RNA Splicing
D004220:Disulfides; C087728:SRPK1 protein, human; D017346:Protein Serine-Threonine Kinases