Efficacy and safety of small intestinal submucosa in dural defect repair in a canine model.
Dural defects are a common problem, and inadequate dural closure can lead to complications. Several types of dural substitute materials have recently been discarded or modified owing to poor biocompatibility or mechanical properties and adverse reactions. The small intestinal submucosa (SIS) is a promising material used in a variety of applications. Based on the limitations of previous studies, we conducted an animal study to evaluate the efficacy and safety of the SIS in preclinical trials. Twenty-four male beagle dogs were subjected to surgical resection to produce dural defects. SIS or autologous dural mater was patched on the dural defect. Gross and histological evaluations were carried out to evaluate the efficacy and safety of the therapy. Our findings demonstrated that the SIS, which stimulated connective and epithelial tissue responses for dural regeneration and functional recovery without immunological rejection, could provide prolonged defect repair and prevent complications. The mechanical properties of the SIS could be adjusted by application of multiple layers, and the biocompatibility of the material was appropriate. Thus, our data suggested that this material may represent an alternative option for clinical treatment of dural defects.
Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China; Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, PR China.
Shu-Kun
SK
He
Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China; Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, PR China; Department of Orthopedics, The Third People's Hospital of Chengdu, Chengdu, Sichuan 610031, PR China.
Jin-Hai
JH
Guo
Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China; Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, PR China.
Zhu-le
ZL
Wang
Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China.
Yi
Y
Zhang
Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan 610083, PR China.
Yun-Hu
YH
Tu
Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China; Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, PR China.
Shi-Zhou
SZ
Wu
Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, PR China.
Fu-Guo
FG
Huang
Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China. Electronic address: xiehuiqi@scu.edu.cn.
Hui-Qi
HQ
Xie
D000818:Animals; D003094:Collagen / Q000378:metabolism; D004195:Disease Models, Animal; D004285:Dogs; D004388:Dura Mater / Q000473:pathology*; D007413:Intestinal Mucosa / Q000637:transplantation*; D007421:Intestine, Small / Q000033:anatomy & histology*; D008297:Male; D013997:Time Factors; D000267:Tissue Adhesions / Q000473:pathology; D016896:Treatment Outcome; D014945:Wound Healing
D003094:Collagen
Autologous dura mater; Dura substitute; Duraplasty; Small intestinal submucosa