Whole-genome sequencing identifies new genetic alterations in meningiomas.
The major known genetic contributor to meningioma formation was NF2, which is disrupted by mutation or loss in about 50% of tumors. Besides NF2, several recurrent driver mutations were recently uncovered through next-generation sequencing. Here, we performed whole-genome sequencing across 7 tumor-normal pairs to identify somatic genetic alterations in meningioma. As a result, Chromatin regulators, including multiple histone members, histone-modifying enzymes and several epigenetic regulators, are the major category among all of the identified copy number variants and single nucleotide variants. Notably, all samples contained copy number variants in histone members. Recurrent chromosomal rearrangements were detected on chromosome 22q, 6p21-p22 and 1q21, and most of the histone copy number variants occurred in these regions. These results will help to define the genetic landscape of meningioma and facilitate more effective genomics-guided personalized therapy.
The State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
Mei
M
Tang
College of Life Science, Sichuan University, Chengdu 610064, China.
Heng
H
Wei
The State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
Lu
L
Han
Department of Neurosurgery, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
Jiaojiao
J
Deng
Department of Neurosurgery, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
Yuelong
Y
Wang
The State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
Meijia
M
Yang
The State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
Yani
Y
Tang
The State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
Gang
G
Guo
Department of Neurosurgery, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
Liangxue
L
Zhou
The State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
Aiping
A
Tong
D002869:Chromosome Aberrations; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D002892:Chromosomes, Human, Pair 22 / Q000235:genetics; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics; D056915:DNA Copy Number Variations; D015321:Gene Rearrangement; D053263:Gene Regulatory Networks; D015894:Genome, Human / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D008577:Meningeal Neoplasms / Q000235:genetics*; D008579:Meningioma / Q000235:genetics*; D009154:Mutation; D025581:Neurofibromin 2 / Q000235:genetics; D020641:Polymorphism, Single Nucleotide
D025581:Neurofibromin 2
chromosome instability; copy number alteration; meningioma; mutation; whole-genome sequencing