Drug-DNA complexation as the key factor in photosensitized thymine dimerization.
The crucial role of photosensitizer@DNA complexation in the formation of cyclobutane pyrimidine dimers (CPDs) has been demonstrated using femtosecond and nanosecond transient absorption and emission measurements in combination with in vitro DNA damage assays. This finding opens the door to re-evaluate the mechanisms involved in CPDs photosensitized by other chemicals.
Instituto Universitario Mixto de Tecnología Química UPV-CSIC, Universitat Politècnica de València, Avda de los Naranjos, s/n, 46022 Valencia, Spain. fbosca@itq.upv.es.
M Consuelo
http://orcid.org/0000-0002-2640-1123
MC
Cuquerella
Instituto Universitario Mixto de Tecnología Química UPV-CSIC, Universitat Politècnica de València, Avda de los Naranjos, s/n, 46022 Valencia, Spain. fbosca@itq.upv.es.
Virginie
http://orcid.org/0000-0002-8205-8892
V
Lhiaubet-Vallet
Instituto Universitario Mixto de Tecnología Química UPV-CSIC, Universitat Politècnica de València, Avda de los Naranjos, s/n, 46022 Valencia, Spain. fbosca@itq.upv.es.
Miguel A
MA
Miranda
Instituto Universitario Mixto de Tecnología Química UPV-CSIC, Universitat Politècnica de València, Avda de los Naranjos, s/n, 46022 Valencia, Spain. fbosca@itq.upv.es.
Francisco
F
Bosca
D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004249:DNA Damage; D019281:Dimerization; D010777:Photochemistry; D017319:Photosensitizing Agents / Q000737:chemistry / Q000378:metabolism*; D011740:Pyrimidine Dimers / Q000737:chemistry; D013941:Thymine / Q000737:chemistry*
D017319:Photosensitizing Agents; D011740:Pyrimidine Dimers; D004247:DNA; D013941:Thymine