Comparison of three superovulation protocols with or without GnRH treatment at the time of artificial insemination on ovarian response and embryo quality in Thai native heifers.
To optimize the superovulation protocol in Thai native cattle, the present research was designed to (1) compare three different protocols designed to induce superstimulation and (2) study the effect of gonadotropin-releasing hormone (GnRH) administration at insemination time (to induce ovulation) on ovarian follicular activities in terms of the number of large follicles, corpora lutea (CLs) and unovulated follicles, and the number and quality of ova/embryos recovered in Thai native heifers. Initially, the estrous cycles of animals (n = 36) at unknown stages were synchronized by two prostaglandin F2α(PGF2α) injections at an interval of 12 days. Follicular development of heifers was randomly superstimulated with one of three different treatment protocols: treatment A-a total of 100 mg of pituitary-derived FSH (pFSH; Folltropin®-V) administered in eight decreasing doses; treatment B-a single dose of 100 mg pFSH dissolved in 30% (w/v) polyvinylpyrrolidone; or treatment C-ablation of all follicles ≥5 mm with a single dose of pFSH. All heifers received PGF2α48 h after the initiation of FSH treatment to induce luteolysis from the previous cycle, and they were twice inseminated at 12 and 24 h after the onset of estrus. Heifers in each treatment were assigned to be injected or not with GnRH at the time of first insemination with frozen/thawed semen to induce ovulation. About 7 days after artificial insemination (AI), ova/embryos were collected and classified. The numbers of large follicles at the onset of estrus were not statistically significantly different; meanwhile, the maximum diameters of follicles at the time of first insemination in treatment C were smaller compared with the other treatment groups (p < 0.001). The administration of GnRH at the first insemination time resulted in a greater number of CLs and fewer unovulated follicles at the time of ova/embryo collection (p = 0.001), which subsequently resulted in a higher number of total ova/embryos recovered (p = 0.030). Among heifers treated with different superstimulation protocols, the ablation of all follicles ≥5 mm in diameter before superstimulation (treatment C) resulted in significantly higher quality of fertilized ova and transferable embryos (p = 0.001). In summary, it could be inferred that GnRH treatment improved ovarian function rather than embryo quality. Dominant follicle ablation prior to superstimulation is preferable for collecting a greater number of transferable embryos.
Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, 40002, Thailand.|Thermo-Tolerant Dairy Cattle Research Group, Khon Kaen University, Khon Kaen, 40002, Thailand.
Vibuntita
V
Chankitisakul
Faculty of Agriculture, Princess of Naradhiwas University, Narathiwat, 98000, Thailand.
Jakkhaphan
J
Pitchayapipatkul
Faculty of Animal Sciences and Agricultural Technology, Silpakorn University Phetchaburi IT Campus, Phetchaburi, 76120, Thailand.
Phirawit
P
Chuawongboon
Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, 40002, Thailand.
Dumrongrak
D
Rakwongrit
Veterinary Research and Development Center (Upper Northeastern Region), Khon Kaen, 40260, Thailand.
Denpong
D
Sakhong
Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, 40002, Thailand.|Thermo-Tolerant Dairy Cattle Research Group, Khon Kaen University, Khon Kaen, 40002, Thailand.
Wuttigrai
W
Boonkum
Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, 40002, Thailand. vthevi@kku.ac.th.|Thermo-Tolerant Dairy Cattle Research Group, Khon Kaen University, Khon Kaen, 40002, Thailand. vthevi@kku.ac.th.
Thevin
T
Vongpralub
D000818:Animals; D002417:Cattle / Q000502:physiology*; D004334:Drug Administration Schedule; D004622:Embryo, Mammalian / Q000187:drug effects; D004971:Estrus / Q000187:drug effects; D005260:Female; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D007315:Insemination, Artificial / Q000662:veterinary*; D008297:Male; D006080:Ovarian Follicle / Q000187:drug effects; D011247:Pregnancy; D011374:Progesterone / Q000008:administration & dosage; D013480:Superovulation / Q000187:drug effects*; D013785:Thailand
D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D005640:Follicle Stimulating Hormone
Embryo quality; GnRH; Ovarian response; Superovulation; Thai native cattle