NanoSOSG: A Nanostructured Fluorescent Probe for the Detection of Intracellular Singlet Oxygen.
A biocompatible fluorescent nanoprobe for singlet oxygen (1O2) detection in biological systems was designed, synthesized, and characterized, that circumvents many of the limitations of the molecular probe Singlet Oxygen Sensor Green®(SOSG). This widely used commercial singlet oxygen probe was covalently linked to a polyacrylamide nanoparticle core using different architectures to optimize the response to1O2. In contrast to its molecular counterpart, the optimum SOSG-based nanoprobe, which we call NanoSOSG, is readily internalized by E. coli cells and does not interact with bovine serum albumin. Furthermore, the spectral characteristics do not change inside cells, and the probe responds to intracellularly generated1O2with an increase in fluorescence.
Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, 08019, Barcelona, Spain.
Rubén
R
Ruiz-González
Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, 08019, Barcelona, Spain.
Roger
R
Bresolí-Obach
Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, 08019, Barcelona, Spain.
Òscar
Ò
Gulías
Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, 08019, Barcelona, Spain.
Montserrat
M
Agut
Department of Chemistry, University of Hull, Cottingham Road, Kingston upon Hull, HU6 7RX, UK.
Huguette
H
Savoie
Department of Chemistry, University of Hull, Cottingham Road, Kingston upon Hull, HU6 7RX, UK.
Ross W
RW
Boyle
Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, 08019, Barcelona, Spain.
Santi
0000-0002-8900-5291
S
Nonell
Department of Chemistry, University of Hull, Cottingham Road, Kingston upon Hull, HU6 7RX, UK.|School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, L3 3AF, UK.
Francesca
F
Giuntini
fluorescent probes; intracellular sensors; nanoparticles; optical sensors; singlet oxygen