Proteomics analysis of latex from Hevea brasiliensis (clone RRIM 600).
The natural rubber latex extracted from the bark of Hevea brasiliensis plays various important roles in today's modern society. Following ultracentrifugation, the latex can be separated into 3 layers: C-serum, lutoids, and rubber particles. Previous studies have shown that a large number of proteins are present in these 3 layers. However, a complete proteome for this important plant is still unavailable. Protein sequences have been recently translated from the completed draft genome database of H. brasiliensis, leading to the creation of annotated protein databases of the following H. brasiliensis biosynthetic pathways: photosynthesis, latex allergens, rubberwood formation, latex biosynthesis, and disease resistance. This research was conducted to identify the proteins contained within the latex by way of de novo sequencing from mass spectral data obtained from the 3 layers of the latex. Peptides from these proteins were fragmented using collision-induced dissociation, higher-energy collisional dissociation, and electron-transfer dissociation activation methods. A large percentage of proteins from the biosynthetic pathways (63% to 100%) were successfully identified. In addition, a total of 1839 unique proteins were identified from the whole translated draft genome database (AnnHBM).
a Analytical Biochemistry Research Centre, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia.
Mohd Afiq Hazlami
MA
Habib
a Analytical Biochemistry Research Centre, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia.
Gan Chee
GC
Yuen
b Anti-Doping Lab Qatar, Sports City Street, Doha, Qatar.
Fazilah
F
Othman
a Analytical Biochemistry Research Centre, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia.
Nurul Nabilah
NN
Zainudin
b Anti-Doping Lab Qatar, Sports City Street, Doha, Qatar.
Aishah Abdul
AA
Latiff
a Analytical Biochemistry Research Centre, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia.
Mohd Nazri
MN
Ismail
D000485:Allergens / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D005591:Chemical Fractionation; D060467:Disease Resistance / Q000235:genetics; D063990:Gene Ontology; D028482:Hevea / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D007840:Latex / Q000737:chemistry*; D058977:Molecular Sequence Annotation; D010788:Photosynthesis / Q000235:genetics; D024301:Plant Bark / Q000737:chemistry; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology; D010940:Plant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D020543:Proteome / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods; D014461:Ultracentrifugation
D000485:Allergens; D007840:Latex; D010940:Plant Proteins; D020543:Proteome
Hevea brasiliensis; biosynthetic pathways; latex; mass spectrometry; plant proteomics; protéomique végétale; rubber latex; spectrométrie de masse; voies biosynthétiques