C21orf57 is a human homologue of bacterial YbeY proteins.
The product of the human C21orf57 (huYBEY) gene is predicted to be a homologue of the highly conserved YbeY proteins found in nearly all bacteria. We show that, like its bacterial and chloroplast counterparts, the HuYbeY protein is an RNase and that it retains sufficient function in common with bacterial YbeY proteins to partially suppress numerous aspects of the complex phenotype of an Escherichia coli ΔybeY mutant. Expression of HuYbeY in Saccharomyces cerevisiae, which lacks a YbeY homologue, results in a severe growth phenotype. This observation suggests that the function of HuYbeY in human cells is likely regulated through specific interactions with partner proteins similarly to the way YbeY is regulated in bacteria.
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
Anubrata
A
Ghosal
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
Caroline
C
Köhrer
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
Vignesh M P
VMP
Babu
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
Kinrin
K
Yamanaka
Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA.
Bryan W
BW
Davies
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
Asha I
AI
Jacob
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
Daniel J
DJ
Ferullo
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
Charley C
CC
Gruber
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
Maarten
M
Vercruysse
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA. Electronic address: gwalker@mit.edu.
Graham C
GC
Walker
D000595:Amino Acid Sequence; D001483:Base Sequence; D002736:Chloroplasts / Q000737:chemistry* / Q000235:genetics*; D017124:Conserved Sequence / Q000235:genetics; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics*; D019143:Evolution, Molecular; D008667:Metalloproteins / Q000737:chemistry* / Q000235:genetics*; D008969:Molecular Sequence Data; D012260:Ribonucleases / Q000737:chemistry* / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D029968:Escherichia coli Proteins; D008667:Metalloproteins; C510617:YbeY protein, E coli; D012260:Ribonucleases; C000619857:YBEY protein, human
C21orf57; RNase; YBEY; Yeast