Immobilization Increases the Stability and Reusability of Pigeon Pea NADP+Linked Glucose-6-Phosphate Dehydrogenase.
Immobilization of enzymes is valuably important as it improves the stability and hence increases the reusability of enzymes. The present investigation is an attempt for immobilization of purified glucose-6-phosphate dehydrogenase from pigeon pea on different matrix. Maximum immobilization was achieved when alginate was used as immobilization matrix. As compared to soluble enzyme the alginate immobilized enzyme exhibited enhanced optimum pH and temperature. The alginate immobilized enzyme displayed more than 80% activity up to 7 continuous reactions and more than 50% activity up to 11 continuous reactions.
Department of Basic Sciences & Humanities, College of Horticulture & Forestry, Central Agricultural University, Pasighat, Arunachal Pradesh, 791102, India. siddharthasingh786@gmail.com.
Siddhartha
S
Singh
Department of Basic Sciences & Humanities, College of Horticulture & Forestry, Central Agricultural University, Pasighat, Arunachal Pradesh, 791102, India.|Department of Agricultural Biotechnology, Bidhan Chandra Krishi Vishwavidyalaya, Mohanpur, Nadia, West Bengal, India.
Amit Kumar
AK
Singh
Department of Basic Sciences & Humanities, College of Horticulture & Forestry, Central Agricultural University, Pasighat, Arunachal Pradesh, 791102, India.
M Chandrakumar
MC
Singh
Department of Basic Sciences & Humanities, College of Horticulture & Forestry, Central Agricultural University, Pasighat, Arunachal Pradesh, 791102, India.
Pramod Kumar
PK
Pandey
D000464:Alginates / Q000737:chemistry*; D036561:Cajanus / Q000201:enzymology*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005954:Glucosephosphate Dehydrogenase / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D009243:NAD / Q000737:chemistry*
D000464:Alginates; D004800:Enzymes, Immobilized; D006603:Hexuronic Acids; D009243:NAD; D020723:Glucuronic Acid; D005954:Glucosephosphate Dehydrogenase
Alginate; Enzyme; Glucose-6-phosphate dehydrogenase; Immobilization; Reusability