Genomic Analysis of a Freshwater Actinobacterium, "CandidatusLimnosphaera aquatica" Strain IMCC26207, Isolated from Lake Soyang.
Strain IMCC26207 was isolated from the surface layer of Lake Soyang in Korea by the dilution-to-extinction culturing method, using a liquid medium prepared with filtered and autoclaved lake water. The strain could neither be maintained in a synthetic medium other than natural freshwater medium nor grown on solid agar plates. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain IMCC26207 formed a distinct lineage in the orderAcidimicrobialesof the phylumActinobacteria. The closest relative among the previously identified bacterial taxa was "CandidatusMicrothrix parvicella" with 16S rRNA gene sequence similarity of 91.7%. Here, the draft genome sequence of strain IMCC26207, a freshwater actinobacterium, is reported with the description of the genome properties and annotation summary. The draft genome consisted of 10 contigs with a total size of 3,316,799 bp and an average G+C content of 57.3%. The IMCC26207 genome was predicted to contain 2,975 protein-coding genes and 51 non-coding RNA genes, including 45 tRNA genes. Approximately 76.8% of the protein coding genes could be assigned with a specific function. Annotation of the IMCC26207 genome showed several traits of adaptation to living in oligotrophic freshwater environments, such as phosphorus-limited condition. Comparative genomic analysis revealed that the genome of strain IMCC26207 was distinct from that of "CandidatusMicrothrix" strains; therefore, we propose the name "CandidatusLimnosphaera aquatica" for this bacterium.
Department of Biological Sciences, Inha University, Incheon 22212, Republic of Korea.
Suhyun
S
Kim
Department of Biological Sciences, Inha University, Incheon 22212, Republic of Korea.
Ilnam
I
Kang
Department of Biological Sciences, Inha University, Incheon 22212, Republic of Korea.
Jang-Cheon
JC
Cho
D039903:Actinobacteria / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics; D001482:Base Composition; D001483:Base Sequence; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005618:Fresh Water / Q000382:microbiology*; D005798:Genes, Bacterial / Q000235:genetics; D016680:Genome, Bacterial / Q000235:genetics*; D060106:Lakes / Q000382:microbiology*; D053858:Metabolic Networks and Pathways / Q000235:genetics; D046529:Microscopy, Electron, Transmission; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
D001426:Bacterial Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012343:RNA, Transfer
Actinobacteria; dilution-to-extinction culturing; freshwater; genome; “Candidatus Limnosphaera”; “Candidatus Microthrix”