Cervical fluid calreticulin and cathepsin-G in pregnancies complicated by preterm prelabor rupture of membranes.
OBJECTIVE
The study aimed to determine the cervical calreticulin and cathepsin-G concentrations in pregnancies complicated by preterm prelabor rupture of membranes (PPROM) with respect to the presence of microbial invasion of the amniotic cavity (MIAC) and intra-amniotic inflammation (IAI).


METHODS
Eighty women with singleton pregnancies complicated by PPROM were included in this study. Cervical and amniotic fluids were obtained at the time of admission, and concentrations of calreticulin and cathepsin-G in cervical fluid were determined using ELISA. The MIAC was defined as a positive PCR analysis for Ureaplasma species, Mycoplasma hominis, and/or Chlamydia trachomatis and/or by positivity for the 16S rRNA gene. IAI was defined as amniotic fluid bedside IL-6 concentrations ≥745 pg/mL Result: Neither women with MIAC nor with IAI had different cervical fluid concentrations of calreticulin (with MIAC: median 18.9 pg/mL vs. without MIAC: median 14.7 pg/mL, p = 0.28; with IAI: median 14.3 pg/mL vs. without IAI: median 15.6 pg/mL, p = 0.57;) or of cathepsin-G (with MIAC: median 30.7 pg/mL vs. without MIAC: median 24.7 pg/mL, p = 0.28; with IAI: median 27.3 pg/mL vs. without IAI: median 25.1 pg/mL, p = 0.80) than women without those complications. No associations between amniotic fluid IL-6 concentrations, gestational age at sampling, and cervical fluid calreticulin and cathepsin-G concentrations were found.


CONCLUSIONS
Cervical fluid calreticulin and cathepsin-G concentrations did not reflect the presence of MIAC or IAI in women with PPROM.
a Department of Clinical Immunology and Allergy , Charles University in Prague, Faculty of Medicine Hradec Kralove, University Hospital Hradec Kralove , Hradec Kralove , Czech Republic.
Ctirad
C
Andrys
b Department of Obstetrics and Gynecology , Charles University in Prague, Faculty of Medicine Hradec Kralove, University Hospital Hradec Kralove , Hradec Kralove , Czech Republic.
Ivana
I
Musilova
a Department of Clinical Immunology and Allergy , Charles University in Prague, Faculty of Medicine Hradec Kralove, University Hospital Hradec Kralove , Hradec Kralove , Czech Republic.
Marcela
M
Drahosova
a Department of Clinical Immunology and Allergy , Charles University in Prague, Faculty of Medicine Hradec Kralove, University Hospital Hradec Kralove , Hradec Kralove , Czech Republic.
Ondrej
O
Soucek
c Institute of Clinical Biochemistry and Diagnostics , University Hospital Hradec Kralove , Hradec Kralove , Czech Republic.
Lenka
L
Pliskova
d Department of Obstetrics and Gynecology, Sahlgrenska Academy , Gothenburg University , Gothenburg , Sweden.|e Department of Genetics and Bioinformatics, Area of Health Data and Digitalization , Norwegian Institute of Public Health , Oslo , Norway.
Bo
B
Jacobsson
f Department of Medical Genetics , Peking University Health Science Center , Peking , China.
Nanbert
N
Zhong
b Department of Obstetrics and Gynecology , Charles University in Prague, Faculty of Medicine Hradec Kralove, University Hospital Hradec Kralove , Hradec Kralove , Czech Republic.|g Biomedical Research Center, University Hospital Hradec Kralove , Hradec Kralove , Czech Republic.
Marian
M
Kacerovsky
D000328:Adult; D000653:Amniotic Fluid / Q000737:chemistry* / Q000378:metabolism / Q000382:microbiology; D015415:Biomarkers / Q000032:analysis; D037282:Calreticulin / Q000032:analysis*; D056649:Cathepsin G / Q000032:analysis*; D002821:Chorioamnionitis / Q000175:diagnosis / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000378:metabolism* / Q000382:microbiology; D005865:Gestational Age; D006801:Humans; D015850:Interleukin-6 / Q000032:analysis; D011247:Pregnancy; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D055815:Young Adult
D015415:Biomarkers; C576483:CALR protein, human; D037282:Calreticulin; D015850:Interleukin-6; C534094:CTSG protein, human; D056649:Cathepsin G
Preterm delivery; amniotic fluid; marker; non-invasive sample