Liquid to liquid extraction and liquid chromatography-tandem mass spectrometry determination of hainanmycin in feed.
Hainanmycin is a new veterinary polyether antibiotic and has few sensitive analytical method in present days. In this study, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) relying on multiple reaction monitoring (MRM) detection was developed for analysis of hainanmycin in animal feed. Feed samples were extracted with ethyl acetate and purified by two steps of liquid-liquid extraction (LLE) to get rid of water solvable matrix and lipids one by one. The final simple was analyzed by LC-MS/MS. The LC mobile phase was composed of 0.1% aqueous formic acid and 0.1% formic acidified acetonitrile by gradient elution. Average recoveries ranged from 74.22% to 87.85%, as determined by spiking with 2.0 (LOQ) ∼2500μgkg-1of hainanmycin. The inter-day and intra-day coefficient of variation was 9.21% to 11.77% and 7.67% to 13.49%, respectively. The limit of detection (LOD) and the limit of quantitation (LOQ) were 0.36μgkg-1and 2.0μgkg-1, respectively.
College of Veterinary Medicine, China Agriculture University, Beijing 100193, China.
Ze Ping
ZP
Wang
College of Veterinary Medicine, China Agriculture University, Beijing 100193, China.
Jian Zhong
JZ
Shen
Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
Robert J
RJ
Linhardt
College of Veterinary Medicine, China Agriculture University, Beijing 100193, China.
Hui
H
Jiang
College of Veterinary Medicine, China Agriculture University, Beijing 100193, China; Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA. Electronic address: pharma2@cau.edu.cn.
Lin Li
LL
Cheng
D000821:Animal Feed / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D004987:Ethers / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D007476:Ionophores / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D057230:Limit of Detection; D016014:Linear Models; D059625:Liquid-Liquid Extraction / Q000379:methods*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
D004987:Ethers; D007476:Ionophores
Feed; Hainanmycin; Liquid chromatography–tandem mass spectrometry; Sample preparation