Student Award for Outstanding Research Winner in the Undergraduate Category for the 2017 Society for Biomaterials Annual Meeting and Exposition, April 5-8, 2017, Minneapolis, Minnesota: Development and characterization of stimuli-responsive hydrogel microcarriers for oral protein delivery.
A family of pH-responsive terpolymers composed of methacrylic acid (MAA), N-vinyl pyrrolidone (NVP), and poly(ethylene glycol) monomethylether monomethacrylate (PEGMMA) have been developed and evaluated for their pH-responsive swelling behavior, protein-loading capabilities, and cytocompatibility. These terpolymer hydrogels, designated as P((MAA-co-NVP)-g-EG), were synthesized with varying PEG chain lengths and monomer feed ratios. The incorporation of MAA into the terpolymer structure was quantified with potentiometric titration. Equilibrium and dynamic swelling studies confirmed the pH-responsive behavior of the hydrogel, with the system remaining collapsed/complexed in acidic pH conditions and swollen/decomplexed in neutral pH conditions. The ability of the hydrogels to partition protein into the swollen network was assessed for two model proteins of varying molecular weight: insulin and porcine growth hormone. Finally, the cytocompatibility of the hydrogels in the presence of two model intestinal cell lines was investigated and confirmed minimal cytotoxicity at and below 2.5 mg/mL. The developed P((MAA-co-NVP)-g-EG) hydrogels exhibit unique properties that could potentially be utilized for drug delivery and separation applications. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1243-1251, 2017.
Department of Biomedical Engineering, The University of Texas at Austin, 107 West Dean Keeton Street Stop C0800, Austin, Texas, 78712.|Institute for Biomaterials, Drug Delivery, and Regenerative Medicine, The University of Texas at Austin, 107 West Dean Keeton Street Stop C0800, Austin, Texas, 78712.
Colleen
C
O'Connor
Department of Biomedical Engineering, The University of Texas at Austin, 107 West Dean Keeton Street Stop C0800, Austin, Texas, 78712.|Institute for Biomaterials, Drug Delivery, and Regenerative Medicine, The University of Texas at Austin, 107 West Dean Keeton Street Stop C0800, Austin, Texas, 78712.
Stephanie
S
Steichen
Department of Biomedical Engineering, The University of Texas at Austin, 107 West Dean Keeton Street Stop C0800, Austin, Texas, 78712.|Institute for Biomaterials, Drug Delivery, and Regenerative Medicine, The University of Texas at Austin, 107 West Dean Keeton Street Stop C0800, Austin, Texas, 78712.|McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 East Dean Keeton Street Stop C0400, Austin, Texas, 78712.|Department of Surgery and Perioperative Care, Dell Medical School, The University of Texas at Austin, 1501 Red River Street, Austin, Texas, 78702.|Division of Pharmaceutics, College of Pharmacy, The University of Texas at Austin, 2409 University Avenue, Austin, Texas, 78712.
Nicholas A
NA
Peppas
D000284:Administration, Oral; D001363:Awards and Prizes; D018938:Caco-2 Cells; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D005260:Female; D020397:Goblet Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D007328:Insulin / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D008297:Male; D008689:Methacrylates / Q000737:chemistry / Q000494:pharmacology; D008910:Minnesota; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D011760:Pyrrolidinones / Q000737:chemistry / Q000494:pharmacology
D004337:Drug Carriers; D020100:Hydrogels; D007328:Insulin; D008689:Methacrylates; D011760:Pyrrolidinones; C008384:methacrylic acid; D011092:Polyethylene Glycols; C042670:N-vinyl-2-pyrrolidinone; C000595209:polyethylene glycol 1000
drug delivery systems; hydrogels; poly(ethylene glycol); proteins; stimuli-responsive polymers