Hepatoprotective role of Ricinus communis leaf extract against d-galactosamine induced acute hepatitis in albino rats.
Ricinus communis (RC) is a traditional medicinal plant which has been used by Chenchu and Yerukula tribes for treating their liver ailments. The present work is aimed to explore the hepatoprotective efficacy of Ricinus communis against d-galactosamine (D-GalN) induced hepatitis rat model and its therapeutic potential compared with standard drug, silymarin (100mg/kg.bw). In vitro antioxidant activity of Methanolic extract of Ricinus communis leaves (MERCL) was assayed through DPPH and H2O2free radical scavenging activity. Qualitative and quantitative analysis of MERCL using HPLC, demonstrated that Rutin was found to be predominant bioactive compound in the extract. Hepatitis was induced by treating the rats with D-GalN at a single intraperitoneal dose of 800mg/kg.bw. Serum markers viz, Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Alkaline phosphatase (ALP) and Malondialdehyde (MDA) levels were significantly increased and the activity levels of antioxidant enzymes such as Superoxide dismutase (SOD),Catalase (CAT), Glutathione reductase (GR), Glutathione peroxidase (GPx), non-enzymatic antioxidant Glutathione (GSH) levels were decreased in the liver of hepatitis induced rats when compared to controls. Pre and post treatment with MERCL significantly altered the enzyme activities, GSH and MDA to normal levels. Histopathological observations also showed protective and curative effects of MERCL against D-GalN intoxication. These results demonstrated that MERCL significantly protected the liver from d-galactosamine induced hepatitis, improved the curative effect in the liver and hence, MERCL can be used as a potent hepatoprotective drug in future.
Division of Molecular Biology, Department of Zoology, Sri Venkateswara University, Tirupati 517502, A.P, India.
Pappithi Ramesh
PR
Babu
Division of Molecular Biology, Department of Zoology, Sri Venkateswara University, Tirupati 517502, A.P, India.
Cherukupalle
C
Bhuvaneswar
Division of Molecular Biology, Department of Zoology, Sri Venkateswara University, Tirupati 517502, A.P, India.
Gandham
G
Sandeep
Division of Molecular Biology, Department of Zoology, Sri Venkateswara University, Tirupati 517502, A.P, India.
Chintha Venkata
CV
Ramaiah
Division of Molecular Biology, Department of Zoology, Sri Venkateswara University, Tirupati 517502, A.P, India. Electronic address: rwudayagiri16@gmail.com.
Wudayagiri
W
Rajendra
D000208:Acute Disease; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D001713:Biphenyl Compounds / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers / Q000494:pharmacology; D005688:Galactosamine; D005978:Glutathione / Q000378:metabolism; D006505:Hepatitis / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D008315:Malondialdehyde / Q000378:metabolism; D000432:Methanol / Q000737:chemistry; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry*; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D017208:Rats, Wistar; D012278:Ricinus / Q000737:chemistry*; D012431:Rutin / Q000032:analysis; D012838:Silymarin / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D015415:Biomarkers; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D010936:Plant Extracts; D020011:Protective Agents; D012838:Silymarin; D017392:Thiobarbituric Acid Reactive Substances; D008315:Malondialdehyde; D012431:Rutin; D005688:Galactosamine; C004931:1,1-diphenyl-2-picrylhydrazyl; D005978:Glutathione; D000432:Methanol
Antioxidant enzymes; HPLC; Hepatoprotective; Ricinus communis; d-Galactosamine