Cryptic bioactivity capacitated by synthetic hybrid plant peptides.
Evolution often diversifies a peptide hormone family into multiple subfamilies, which exert distinct activities by exclusive interaction with specific receptors. Here we show that systematic swapping of pre-existing variation in a subfamily of plant CLE peptide hormones leads to a synthetic bifunctional peptide that exerts activities beyond the original subfamily by interacting with multiple receptors. This approach provides new insights into the complexity and specificity of peptide signalling.
Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
Yuki
Y
Hirakawa
Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464-8602, Japan.
Hidefumi
H
Shinohara
Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.|Department of Chemistry, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464-8602, Japan.
Kai
K
Welke
Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.|Department of Chemistry, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464-8602, Japan.
Stephan
0000-0003-4995-4991
S
Irle
Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464-8602, Japan.
Yoshikatsu
Y
Matsubayashi
Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.|Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464-8602, Japan.|Department of Biology, University of Washington, Seattle, Washington 98195, USA.|Howard Hughes Medical Institute, University of Washington, Seattle, Washington 98195, USA.
Keiko U
0000-0002-6168-427X
KU
Torii
Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.|Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464-8602, Japan.
Naoyuki
N
Uchida
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000138:chemical synthesis / Q000502:physiology*; D044822:Biodiversity; D019143:Evolution, Molecular; D008024:Ligands; D062105:Molecular Docking Simulation; D015394:Molecular Structure; D036361:Peptide Hormones / Q000138:chemical synthesis / Q000502:physiology*; D010937:Plant Growth Regulators / Q000138:chemical synthesis / Q000502:physiology*; D030821:Plants, Genetically Modified; D013234:Stem Cells / Q000378:metabolism / Q000502:physiology*; D013329:Structure-Activity Relationship; D013379:Substrate Specificity / Q000502:physiology
D029681:Arabidopsis Proteins; D008024:Ligands; D036361:Peptide Hormones; D010937:Plant Growth Regulators