Differential lactate and cholesterol synthetic activities in XY and XX Sertoli cells.
SRY, a sex-determining gene, induces testis development in chromosomally female (XX) individuals. However, mouse XX Sertoli cells carrying Sry (XX/Sry Sertoli cells) are incapable of fully supporting germ cell development, even when the karyotype of the germ cells is XY. While it has therefore been assumed that XX/Sry Sertoli cells are not functionally equivalent to XY Sertoli cells, it has remained unclear which specific functions are affected. To elucidate the functional difference, we compared the gene expression of XY and XX/Sry Sertoli cells. Lactate and cholesterol metabolisms, essential for nursing the developing germ cells, were down-regulated in XX/Sry cells, which appears to be caused at least in part by the differential expression of histone modification enzymes SMCX/SMCY (H3K4me3 demethylase) and UTX/UTY (H3K27me3 demethylase) encoded by the sex chromosomes. We suggest that down-regulation of lactate and cholesterol metabolism that may be due to altered epigenetic modification affects the nursing functions of XX/Sry Sertoli cells.
Division of Molecular Life Sciences, Graduate School of Systems Life Science, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.
Yurina
Y
Shishido
Division of Molecular Life Sciences, Graduate School of Systems Life Science, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.|Department of Molecular Biology, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.
Takashi
T
Baba
Division of Molecular Life Sciences, Graduate School of Systems Life Science, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.|Division of Bioinformatics, Medical Institute of Bioregulation, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.|AMED-CREST, Japan Agency for Medical Research and Development, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.
Tetsuya
T
Sato
Division of Molecular Life Sciences, Graduate School of Systems Life Science, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.|Department of Molecular Biology, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.
Yuichi
Y
Shima
Department of Molecular Biology, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.
Kanako
K
Miyabayashi
Division of Molecular Life Sciences, Graduate School of Systems Life Science, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.
Miki
M
Inoue
Department of Orthopaedics, Gifu University Graduate School of Medicine, Yanagito 1-1, Gifu, 501-1194, Japan.
Haruhiko
H
Akiyama
Department of Biological Sciences, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, 226-8501, Japan.
Hiroshi
H
Kimura
Department of Veterinary Anatomy, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan.
Yoshiakira
Y
Kanai
Graduate School of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima, 739-8521, Japan.
Yasuhiro
Y
Ishihara
Department of Biochemistry, Showa University School of Medicine, Hatanodai 1-5-8, Shinagawa-ku, Tokyo 142-8555, Japan.
Shogo
S
Haraguchi
Department of Biochemistry, Showa University School of Medicine, Hatanodai 1-5-8, Shinagawa-ku, Tokyo 142-8555, Japan.
Akira
A
Miyazaki
Centre for Functional Genomics and Bio-Chips, Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Zaloska 4, SI-1000 Ljubljana, Slovenia.
Damjana
D
Rozman
Graduate School of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima, 739-8521, Japan.
Takeshi
T
Yamazaki
Molecular Recognition Research Center, Korea Institute of Science and Technology, Seoul, 02792, Korea.
Man-Ho
MH
Choi
Division of Molecular Life Sciences, Graduate School of Systems Life Science, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.|Research Center for Transomics Medicine, Medical Institute of Bioregulation, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.
Yasuyuki
Y
Ohkawa
Division of Molecular Life Sciences, Graduate School of Systems Life Science, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.|Division of Bioinformatics, Medical Institute of Bioregulation, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.|AMED-CREST, Japan Agency for Medical Research and Development, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.
Mikita
M
Suyama
Division of Molecular Life Sciences, Graduate School of Systems Life Science, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.|Department of Molecular Biology, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.
Ken-Ichirou
KI
Morohashi
D000818:Animals; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism*; D012734:Disorders of Sex Development / Q000235:genetics / Q000378:metabolism*; D005260:Female; D042421:Histone Code; D056466:Histone Demethylases / Q000235:genetics / Q000378:metabolism; D059785:Karyotype; D019344:Lactic Acid / Q000378:metabolism*; D008297:Male; D051379:Mice; D012708:Sertoli Cells / Q000378:metabolism* / Q000473:pathology; D051876:Sex-Determining Region Y Protein / Q000235:genetics / Q000378:metabolism; D014960:X Chromosome / Q000235:genetics; D014998:Y Chromosome / Q000235:genetics
D051876:Sex-Determining Region Y Protein; D019344:Lactic Acid; D002784:Cholesterol; D056466:Histone Demethylases