Gastroprotective and antielastase effects of protein inhibitors from Erythrina velutina seeds in an experimental ulcer model.
Trypsin and chymotrypsin inhibitors from Erythrina velutina seeds have been previously isolated by our group. In previous studies using a sepsis model, we demonstrated the antitumor and anti-inflammatory action of these compounds. This study aimed to evaluate the gastroprotective and antielastase effects of protein inhibitors from E. velutina seeds in an experimental stress-induced ulcer model. Two protein isolates from E. velutina seeds, with antitrypsin (PIAT) and antichymotrypsin (PIAQ) activities, were tested. Both protein isolates showed a high affinity and inhibitory effect against human neutrophil elastase, with 84% and 85% inhibition, respectively. Gastric ulcer was induced using ethanol (99%) in 6 groups of animals (female Wistar rats, n = 6). Before ulcer induction, these animals were treated for 5 days with one of the following: (1) PIAT (0.2 mg·kg-1), (2) PIAT (0.4 mg·kg-1), (3) PIAQ (0.035 mg·kg-1), (4) ranitidine hydrochloride (50 mg·kg-1), (5) saline solution (0.9%), or (6) no intervention (sham). Both PIAT and PIAQ protected gastric mucosa, preventing hemorrhagic lesions, edema, and mucus loss. No histologic toxic effects of PIAT or PIAQ were seen in liver and pancreatic cells. Our results show that protein isolates from E. velutina seeds have potential gastroprotective effects, placing these compounds as natural candidates for gastric ulcer prevention.
a Biochemistry Department, Biosciences Center, Federal University of Rio Grande do Norte, 59078-970 Natal, RN, Brazil.
Vanessa Cristina
VC
Oliveira de Lima
a Biochemistry Department, Biosciences Center, Federal University of Rio Grande do Norte, 59078-970 Natal, RN, Brazil.
Richele Janaína
RJ
de Araújo Machado
a Biochemistry Department, Biosciences Center, Federal University of Rio Grande do Norte, 59078-970 Natal, RN, Brazil.
Norberto Kássio
NK
Vieira Monteiro
a Biochemistry Department, Biosciences Center, Federal University of Rio Grande do Norte, 59078-970 Natal, RN, Brazil.
Ibson Lucas
IL
de Lyra
b Morphology Department, Biosciences Center, Federal University of Rio Grande do Norte, 59078-970 Natal, RN, Brazil.
Christina
C
da Silva Camillo
a Biochemistry Department, Biosciences Center, Federal University of Rio Grande do Norte, 59078-970 Natal, RN, Brazil.
Alexandre
A
Coelho Serquiz
a Biochemistry Department, Biosciences Center, Federal University of Rio Grande do Norte, 59078-970 Natal, RN, Brazil.
Adeliana
A
Silva de Oliveira
a Biochemistry Department, Biosciences Center, Federal University of Rio Grande do Norte, 59078-970 Natal, RN, Brazil.
Fabíola Patrícia
FP
da Silva Rufino
c Nutrition Department Center for Health Sciences, Federal University of Rio Grande do Norte, 59078-970 Natal, RN, Brazil.
Bruna
B
Leal Lima Maciel
d Molecular Biology and Genetics Department, Center for Health Sciences, Federal University of Rio Grande do Norte, 59078-970 Natal, RN, Brazil.
Adriana
A
Ferreira Uchôa
a Biochemistry Department, Biosciences Center, Federal University of Rio Grande do Norte, 59078-970 Natal, RN, Brazil.
Elizeu
E
Antunes Dos Santos
c Nutrition Department Center for Health Sciences, Federal University of Rio Grande do Norte, 59078-970 Natal, RN, Brazil.
Ana Heloneida
AH
de Araújo Morais
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D004895:Erythrina / Q000737:chemistry*; D000431:Ethanol; D005260:Female; D005753:Gastric Mucosa / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D005765:Gastrointestinal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D019272:Leukocyte Elastase / Q000037:antagonists & inhibitors / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry; D011899:Ranitidine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry; D013276:Stomach Ulcer / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control*
D000893:Anti-Inflammatory Agents; D004791:Enzyme Inhibitors; D005765:Gastrointestinal Agents; D010936:Plant Extracts; D000431:Ethanol; D011899:Ranitidine; D019272:Leukocyte Elastase
activité du neutrophile; antichymotrypsin; antichymotrypsine; antitrypsin; antitrypsine; gastric ulcer; inhibiteurs des protéases à sérine; neutrophil activity; serine protease inhibitors; ulcère gastrique