Simultaneous determination of 13 carotenoids by a simple C18 column-based ultra-high-pressure liquid chromatography method for carotenoid profiling in the astaxanthin-accumulating Haematococcus pluvialis.
A simple ultra-high-pressure liquid chromatography (UHPLC) method for rapidly and simultaneously identifying thirteen carotenoids in Haematococcus pluvialis was developed in this study. The method is capable of effectively separating two astaxanthin isomers, two ζ-carotene isomers, and three phytoene isomers on two simple C18 columns within 9 and 12min only by using methanol and acetonitrile, respectively. To our best knowledge, this is the rapidest method for these carotenoid isomers, currently. Using this method, carotenoid profiling in the astaxanthin-accumulating H. pluvialis under environmental stresses was successfully carried out. Results indicated that carotenoid biosynthesis was differentially perturbed by environmental stresses, indicating that this simple and rapid method is suitable to not only bacterial but also algal samples, with potential applications for a wide range of samples from plant to animal. Finally, possible reasons for the elution order of carotenoids were studied.
School of Life Sciences, Tsinghua University, Beijing 100084, China; Shenzhen Public Platform of Screening & Application of Marine Microbial Resources, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, PR China; Shenzhen Key Laboratory for Coastal Ocean Dynamic and Environment, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, PR China.
Hui
H
Jin
Shenzhen Public Platform of Screening & Application of Marine Microbial Resources, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, PR China; Shenzhen Key Laboratory for Coastal Ocean Dynamic and Environment, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, PR China.
Yong Min
YM
Lao
Shenzhen Public Platform of Screening & Application of Marine Microbial Resources, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, PR China; Shenzhen Key Laboratory for Coastal Ocean Dynamic and Environment, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, PR China.
Jin
J
Zhou
School of Life Sciences, Tsinghua University, Beijing 100084, China; Shenzhen Public Platform of Screening & Application of Marine Microbial Resources, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, PR China; Shenzhen Key Laboratory for Coastal Ocean Dynamic and Environment, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, PR China.
Huai Jin
HJ
Zhang
Shenzhen Public Platform of Screening & Application of Marine Microbial Resources, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, PR China; Shenzhen Key Laboratory for Coastal Ocean Dynamic and Environment, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, PR China. Electronic address: caizh@sz.tsinghua.edu.cn.
Zhong Hua
ZH
Cai
D002138:Calibration; D002338:Carotenoids / Q000032:analysis* / Q000096:biosynthesis / Q000737:chemistry; D000460:Chlorophyta / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D012015:Reference Standards; D024341:Xanthophylls / Q000378:metabolism
D024341:Xanthophylls; D002338:Carotenoids; C005948:astaxanthine
C18 column; Carotenoid profiling; Environmental stress; Haematococcus pluvialis; Stationary phase