A biological method for in-situ synthesis of hydroxyapatite-coated magnetite nanoparticles using Enterobacter aerogenes: Characterization and acute toxicity assessments.
Hydroxyapatite (HA)-coated magnetite nanoparticles (MNPs) are being widely investigated for various applications in medical engineering and wastewater treatment. In this work, the MNPs were thoroughly coated by bacterial synthesized HA nanoparticles during biomineralization process using Enterobacter aerogenes. The resulting bacterial-induced precipitate was then calcined at 600°C and investigated with respect to structural characteristics, particle size and magnetic strength by XRD, FT-IR, SEM, EDS, TEM and VSM analyses. The effects of MNPs and HA-coated MNPs (HA-MNPs) on the viability of human MCF-7 cell lines were also investigated via mitochondrial activity test (MTT) and lactate dehydrogenase (LDH) assays. The powder characterization results showed appropriate structural properties for HA-MNPs samples. The particles diameter size of the MNPs and HA-MNPs were in the range of 3-25nm and 20-80nm, respectively. The biologically-synthesized HA-MNPs formed a stable suspension in water while keeping their magnetic property. The saturation magnetization (Ms) of HA-MNPs was measured at ~10emug-1which was in good agreement with the structural composition of this sample. Finally, the results of the cell lines viability indicated that coating of toxic MNPs via biomineralization was a promising approach in order to synthesize bio-compatible magnetic nanoparticles with suitable physical and chemical structural characteristics. The toxicity level of MNPs was reduced by 10 fold when coated by bacterial-synthesized HA.
Faculty of Chemical Engineering, Babol Noshirvani University of Technology, P.O. Box: 484, Postal Code: 47148-71167 Babol, Mazandaran, Iran.
Elham
E
Ahmadzadeh
Faculty of Chemical Engineering, Babol Noshirvani University of Technology, P.O. Box: 484, Postal Code: 47148-71167 Babol, Mazandaran, Iran. Electronic address: f.talebnia@nit.ac.ir.
Farid
F
Talebnia Rowshan
Faculty of Chemical Engineering, Babol Noshirvani University of Technology, P.O. Box: 484, Postal Code: 47148-71167 Babol, Mazandaran, Iran.
Morteza
M
Hosseini
D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D017886:Durapatite / Q000633:toxicity*; D021902:Enterobacter aerogenes / Q000187:drug effects*; D006801:Humans; D061986:MCF-7 Cells; D060328:Magnetic Phenomena; D058185:Magnetite Nanoparticles / Q000633:toxicity* / Q000648:ultrastructure; D010316:Particle Size; D017550:Spectroscopy, Fourier Transform Infrared; D023382:Toxicity Tests, Acute; D014961:X-Ray Diffraction
D058185:Magnetite Nanoparticles; D017886:Durapatite
Coating; Cytotoxicity; Enterobacter aerogenes; Hydroxyapatite; Magnetite nanoparticles