Chromosomal distribution patterns of the (AC)10microsatellite and other repetitive sequences, and their use in chromosome rearrangement analysis of species of the genus Avena.
Fluorescence in situ hybridization (FISH) was used to determine the physical location of the (AC)10microsatellite in metaphase chromosomes of six diploid species (AA or CC genomes), two tetraploid species (AACC genome), and five cultivars of two hexaploid species (AACCDD genome) of the genus Avena, a genus in which genomic relationships remain obscure. A preferential distribution of the (AC)10microsatellite in the pericentromeric and interstitial regions was seen in both the A- and D-genome chromosomes, while in C-genome chromosomes the majority of signals were located in the pericentromeric heterochromatic regions. New large chromosome rearrangements were detected in two polyploid species: an intergenomic translocation involving chromosomes 17AL and 21DS in Avena sativa 'Araceli' and another involving chromosomes 4CL and 21DS in the analyzed cultivars of Avena byzantina. The latter 4CL-21DS intergenomic translocation differentiates clearly between A. sativa and A. byzantina. Searches for common hybridization patterns on the chromosomes of different species revealed chromosome 10A of Avena magna and 21D of hexaploid oats to be very similar in terms of the distribution of 45S and Am1 sequences. This suggests a common origin for these chromosomes and supports a CCDD rather than an AACC genomic designation for this species.
Department of Biomedicine and Biotechnology, 28871 Alcalá de Henares, Madrid, Spain.|Department of Biomedicine and Biotechnology, 28871 Alcalá de Henares, Madrid, Spain.
Araceli
A
Fominaya
Department of Biomedicine and Biotechnology, 28871 Alcalá de Henares, Madrid, Spain.|Department of Biomedicine and Biotechnology, 28871 Alcalá de Henares, Madrid, Spain.
Yolanda
Y
Loarce
Department of Biomedicine and Biotechnology, 28871 Alcalá de Henares, Madrid, Spain.|Department of Biomedicine and Biotechnology, 28871 Alcalá de Henares, Madrid, Spain.
Alexander
A
Montes
Department of Biomedicine and Biotechnology, 28871 Alcalá de Henares, Madrid, Spain.|Department of Biomedicine and Biotechnology, 28871 Alcalá de Henares, Madrid, Spain.
Esther
E
Ferrer
D018554:Avena / Q000235:genetics*; D032461:Chromosomes, Plant / Q000235:genetics* / Q000648:ultrastructure; D018744:DNA, Plant / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004171:Diploidy; D015321:Gene Rearrangement; D018745:Genome, Plant; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D018895:Microsatellite Repeats; D008938:Mitosis; D009693:Nucleic Acid Hybridization; D011123:Polyploidy; D012091:Repetitive Sequences, Nucleic Acid; D013696:Temperature; D014178:Translocation, Genetic
D018744:DNA, Plant; D004275:DNA, Ribosomal
ADN répété; Avena; chromosome structure; fluorescence in situ hybridization; hybridation in situ en fluorescence; repetitive DNA; structure des chromosomes