Proteomic Analysis of Various Rat Ocular Tissues after Ischemia-Reperfusion Injury and Possible Relevance to Acute Glaucoma.
Glaucoma is a group of eye diseases that can cause vision loss and optical nerve damage. To investigate the protein expression alterations in various intraocular tissues (i.e., the cornea, conjunctiva, uvea, retina, and sclera) during ischemia-reperfusion (IR) injury, this study performed a proteomic analysis to qualitatively investigate such alterations resulting from acute glaucoma. The IR injury model combined with the proteomic analysis approach of two-dimensional difference gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to monitor the protein expression alterations in two groups of specimens (an IR injury group and a control group). The analysis results revealed 221 unique differentially expressed proteins of a total of 1481 proteins in the cornea between the two groups. In addition, 97 of 1206 conjunctival proteins, 90 of 1354 uveal proteins, 61 of 1180 scleral proteins, and 37 of 1204 retinal proteins were differentially expressed. These findings imply that different ocular tissues have different tolerances against IR injury. To sum up, this study utilized the acute glaucoma model combined with 2D-DIGE and MALDI-TOF MS to investigate the IR injury affected protein expression on various ocular tissues, and based on the ratio of protein expression alterations, the alterations in the ocular tissues were in the following order: the cornea, conjunctiva, uvea, sclera, and retina.
Institute of Bioinformatics and Structural Biology & Department of Medical Sciences, National Tsing Hua University, Hsinchu 300, Taiwan. dful690@yahoo.com.tw.
Hsin-Yi
HY
Chen
Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu 300, Taiwan. chouhc@mail.nhcue.edu.tw.|Center for Teacher Education, National Tsing Hua University, Hsinchu 300, Taiwan. chouhc@mail.nhcue.edu.tw.|Department of Applied Science, National Hsinchu University of Education, Hsinchu 300, Taiwan. chouhc@mail.nhcue.edu.tw.
Hsiu-Chuan
HC
Chou
Gynecologic Oncology Section Department of Obstetrics and Gynecology, Hsinchu Mackay Memorial Hospital, Hsinchu 300, Taiwan. testisfine@gmail.com.
Shing-Jyh
SJ
Chang
Institute of Bioinformatics and Structural Biology & Department of Medical Sciences, National Tsing Hua University, Hsinchu 300, Taiwan. nakyla1215@gmail.com.
En-Chi
EC
Liao
Institute of Bioinformatics and Structural Biology & Department of Medical Sciences, National Tsing Hua University, Hsinchu 300, Taiwan. peach0722@hotmail.com.tw.
Yi-Ting
YT
Tsai
Institute of Bioinformatics and Structural Biology & Department of Medical Sciences, National Tsing Hua University, Hsinchu 300, Taiwan. t91050127@hotmail.com.tw.
Yu-Shan
YS
Wei
Institute of Bioinformatics and Structural Biology & Department of Medical Sciences, National Tsing Hua University, Hsinchu 300, Taiwan. j77801@hotmail.com.
Ji-Min
JM
Li
Institute of Bioinformatics and Structural Biology & Department of Medical Sciences, National Tsing Hua University, Hsinchu 300, Taiwan. lishlin0207@gmail.com.
Li-Hsun
LH
Lin
Institute of Bioinformatics and Structural Biology & Department of Medical Sciences, National Tsing Hua University, Hsinchu 300, Taiwan. eva1018cat@yahoo.com.tw.
Meng-Wei
MW
Lin
Institute of Bioinformatics and Structural Biology & Department of Medical Sciences, National Tsing Hua University, Hsinchu 300, Taiwan. chipmunk210@hotmail.com.
Ying-Jen
YJ
Chen
Institute of Bioinformatics and Structural Biology & Department of Medical Sciences, National Tsing Hua University, Hsinchu 300, Taiwan. leo931016@yahoo.com.tw.
Yu-Sheng
YS
Chen
Institute of Bioinformatics and Structural Biology & Department of Medical Sciences, National Tsing Hua University, Hsinchu 300, Taiwan. jennylin40728@yahoo.com.tw.
Chih-Chun
CC
Lin
Institute of Bioinformatics and Structural Biology & Department of Medical Sciences, National Tsing Hua University, Hsinchu 300, Taiwan. woowoow0320@gmail.com.
Yi-Shiuan
YS
Wang
Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu 300, Taiwan. aaddch@gmail.com.|Department of Ophthalmology, National Taiwan University Hospital Hsin-Chu Branch, Hsinchu 300, Taiwan. aaddch@gmail.com.
Mei-Lan
ML
Ko
Institute of Bioinformatics and Structural Biology & Department of Medical Sciences, National Tsing Hua University, Hsinchu 300, Taiwan. hlchan@life.nthu.edu.tw.
Hong-Lin
HL
Chan
D000208:Acute Disease; D000818:Animals; D003228:Conjunctiva / Q000378:metabolism; D003315:Cornea / Q000378:metabolism; D004195:Disease Models, Animal; D005901:Glaucoma / Q000209:etiology* / Q000378:metabolism*; D020543:Proteome; D040901:Proteomics / Q000379:methods; D051381:Rats; D015427:Reperfusion Injury / Q000150:complications* / Q000378:metabolism*; D015203:Reproducibility of Results; D012160:Retina / Q000378:metabolism; D012590:Sclera / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D057825:Two-Dimensional Difference Gel Electrophoresis
D020543:Proteome
acute glaucoma; cornea; difference gel electrophoresis (DIGE); ischemia–reperfusion (IR) model; matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF); proteomics