Redox responsive albumin autogenic nanoparticles for the delivery of cancer drugs.
The present study explores preparation and characterization of redox sensitive albumin autogenic nanoparticles (ANPs) for drug delivery applications. Human serum albumin nanoparticles are prepared by desolvation method. The particles are stabilized through self-crosslinking and no external stabilizers are involved in the preparation. ANPs are then subjected to Camptothecin (CPT) drug loading. Experiments on in vitro and in vivo release profile, cytotoxic and cytocompatability, hemocompatability, blood clearance, tracking and bio imaging are studied in detail. The redox sensitive and drug release properties of ANPs studied in the presence of glutathione. Results on the physical, chemical and instrumental characterization warrant the property of the nanoparticles. ANPs obtained in the present study is biocompatible, biodegradable, effectively entangle the chosen drug, release the drug in the controlled manner, sensitive to reducing environment, nil toxicity and appreciable uptake by cells. In the current scenario on the requirement of a drug carrier with redox sensitive property to encounter cancer cells, the results of the present study on albumin nanoparticles with redox sensitivity is smart and pave the way in the cancer therapeutics.
Biological Material Laboratory, Microbiology Division, CSIR-CLRI, Adyar, Chennai 600 020, Tamil Nadu, India.
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Thirupathi Kumara Raja
Biological Material Laboratory, Microbiology Division, CSIR-CLRI, Adyar, Chennai 600 020, Tamil Nadu, India.
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Prakash
Biological Material Laboratory, Microbiology Division, CSIR-CLRI, Adyar, Chennai 600 020, Tamil Nadu, India. Electronic address: gnanamani3@gmail.com.
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Gnanamani
D000418:Albumins / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D002166:Camptothecin / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods; D065546:Drug Liberation; D005978:Glutathione / Q000737:chemistry; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D017510:Protein Folding
D000418:Albumins; D000970:Antineoplastic Agents; D004337:Drug Carriers; D005978:Glutathione; D002166:Camptothecin
Autogenic material; Drug delivery; Nanoparticles; Oxidative refolding; Serum albumin