Toxicology of the aqueous extract from the flowers of Butea monosperma Lam. and it's metabolomics in yeast cells.
Due to lack of scientific evidence for the safety of Butea monosperma (Fabaceae), our study aimed to carry out its toxicological profile and to identify its metabolic pattern in yeast cell. The effect of aqueous extract of B. monosperma flower on glucose uptake in yeast cell was evaluated through optimizing pH, temperature, incubation time, substrate concentration and kinetic parameters. Further, the metabolic pattern of extract as such and in yeast cell were analyzed by gas chromatography-mass spectrometry. Mice were administered aqueous extract up to 6000 and 4000 mg/kg for acute oral and intraperitoneal toxicity, respectively, while up to 4500 mg/kg for sub-acute oral toxicity (30 days). Elongation in the lag and log phase was observed in yeast cells supplemented with extract as compared to control. A maximum of 184.9% glucose uptake was observed whereas kinetic parameters (Kmand Vmax) were 1.38 and 41.91 mol/s, respectively. Out of 75 metabolites found in the extract, 14 and 18 metabolites were utilized by yeast cell after 15 and 30 min of incubation, respectively. The LD50of extract administered through intraperitoneal route was estimated to be 3500 mg/kg. The extract did not elicit any significant difference (P ≥ 0.05) in weight gain, food consumption, water intake, hematological, biochemical parameters and histological changes as compared to the normal control. Results ascertained the safety of B. monosperma flower extract which can be explored as potential candidates for the development of anti-diabetic phytopharmaceuticals.
Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
Washim
W
Khan
Department of Biotechnology, Graphic Era University, Dehradun 248002, Uttarakhand, India.
Shreesh
S
Gupta
Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India. Electronic address: sahmad_jh@yahoo.co.in.
Sayeed
S
Ahmad
D000818:Animals; D031290:Butea / Q000737:chemistry*; D003470:Culture Media; D035264:Flowers / Q000737:chemistry*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D055432:Metabolomics; D051379:Mice; D008810:Mice, Inbred C57BL; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*; D011897:Random Allocation; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D003470:Culture Media; D005656:Fungal Proteins; D010936:Plant Extracts; D005947:Glucose
Baker's yeast; GC-MS; Optimization; Palash; Pattern recognition; Toxicity