Cleaning with a wet sterile gauze significantly reduces contamination of sutures, instruments, and surgical gloves in an ex-vivo pelvic flexure enterotomy model in horses.
The objective of this study was to investigate whether cleaning surgical materials used to close pelvic flexure enterotomies with a wet sterile gauze will reduce contamination and whether the use of a full thickness appositional suture pattern (F) or a partial thickness inverting (or Cushing) suture pattern (C) would make a difference in the level of contamination. Large colon specimens were assigned to group F or C and divided into subgroups N and G. In group G, a wet sterile gauze was passed over the suture material, another over the instruments, and another over the gloves. In group N, no treatment was applied. The bacterial concentration was measured by optical density (OD) at 24 h. The OD of subgroup CG was lower than that of subgroup CN (P= 0.019). The OD of subgroup FG was lower than that of subgroup FN (P= 0.02). The OD of subgroups CG, CN, FG, and FN was lower than that of the negative control (P< 0.003,P< 0.001,P< 0.001, andP< 0.00). The use of a sterile wet gauze significantly reduced contamination of suture materials. A partial thickness inverting suture pattern did not produce less contamination than a full thickness appositional suture pattern.
Department of Veterinary Sciences, University of Turin, Largo P. Braccini 2-5, 10095 Grugliasco (TO), Italy (Giusto, Tramuta, Caramello, Comino, Nebbia, Robino, Grego, Gandini); Institute for Aging and Chronic Disease, School of Veterinary Science, University of Liverpool, Leahurst, Neston, Cheshire, United Kingdom (Singer). Marco Gandini and Elena Grego share last name authorship.
Gessica
G
Giusto
Department of Veterinary Sciences, University of Turin, Largo P. Braccini 2-5, 10095 Grugliasco (TO), Italy (Giusto, Tramuta, Caramello, Comino, Nebbia, Robino, Grego, Gandini); Institute for Aging and Chronic Disease, School of Veterinary Science, University of Liverpool, Leahurst, Neston, Cheshire, United Kingdom (Singer). Marco Gandini and Elena Grego share last name authorship.
Clara
C
Tramuta
Department of Veterinary Sciences, University of Turin, Largo P. Braccini 2-5, 10095 Grugliasco (TO), Italy (Giusto, Tramuta, Caramello, Comino, Nebbia, Robino, Grego, Gandini); Institute for Aging and Chronic Disease, School of Veterinary Science, University of Liverpool, Leahurst, Neston, Cheshire, United Kingdom (Singer). Marco Gandini and Elena Grego share last name authorship.
Vittorio
V
Caramello
Department of Veterinary Sciences, University of Turin, Largo P. Braccini 2-5, 10095 Grugliasco (TO), Italy (Giusto, Tramuta, Caramello, Comino, Nebbia, Robino, Grego, Gandini); Institute for Aging and Chronic Disease, School of Veterinary Science, University of Liverpool, Leahurst, Neston, Cheshire, United Kingdom (Singer). Marco Gandini and Elena Grego share last name authorship.
Francesco
F
Comino
Department of Veterinary Sciences, University of Turin, Largo P. Braccini 2-5, 10095 Grugliasco (TO), Italy (Giusto, Tramuta, Caramello, Comino, Nebbia, Robino, Grego, Gandini); Institute for Aging and Chronic Disease, School of Veterinary Science, University of Liverpool, Leahurst, Neston, Cheshire, United Kingdom (Singer). Marco Gandini and Elena Grego share last name authorship.
Patrizia
P
Nebbia
Department of Veterinary Sciences, University of Turin, Largo P. Braccini 2-5, 10095 Grugliasco (TO), Italy (Giusto, Tramuta, Caramello, Comino, Nebbia, Robino, Grego, Gandini); Institute for Aging and Chronic Disease, School of Veterinary Science, University of Liverpool, Leahurst, Neston, Cheshire, United Kingdom (Singer). Marco Gandini and Elena Grego share last name authorship.
Patrizia
P
Robino
Department of Veterinary Sciences, University of Turin, Largo P. Braccini 2-5, 10095 Grugliasco (TO), Italy (Giusto, Tramuta, Caramello, Comino, Nebbia, Robino, Grego, Gandini); Institute for Aging and Chronic Disease, School of Veterinary Science, University of Liverpool, Leahurst, Neston, Cheshire, United Kingdom (Singer). Marco Gandini and Elena Grego share last name authorship.
Ellen
E
Singer
Department of Veterinary Sciences, University of Turin, Largo P. Braccini 2-5, 10095 Grugliasco (TO), Italy (Giusto, Tramuta, Caramello, Comino, Nebbia, Robino, Grego, Gandini); Institute for Aging and Chronic Disease, School of Veterinary Science, University of Liverpool, Leahurst, Neston, Cheshire, United Kingdom (Singer). Marco Gandini and Elena Grego share last name authorship.
Elena
E
Grego
Department of Veterinary Sciences, University of Turin, Largo P. Braccini 2-5, 10095 Grugliasco (TO), Italy (Giusto, Tramuta, Caramello, Comino, Nebbia, Robino, Grego, Gandini); Institute for Aging and Chronic Disease, School of Veterinary Science, University of Liverpool, Leahurst, Neston, Cheshire, United Kingdom (Singer). Marco Gandini and Elena Grego share last name authorship.
Marco
M
Gandini
D000818:Animals; D001419:Bacteria; D003106:Colon / Q000601:surgery*; D004766:Enterostomy / Q000662:veterinary*; D005932:Gloves, Surgical / Q000382:microbiology / Q000662:veterinary*; D006736:Horses / Q000601:surgery*; D013525:Surgical Instruments / Q000382:microbiology / Q000662:veterinary*; D013537:Sutures / Q000382:microbiology*