Enrichment of Specifically Labeled Proteins by an Immobilized Host Molecule.
Chemical proteomics relies primarily on click-chemistry-based protein labeling and biotin-streptavidin enrichment, but these techniques have inherent limitations. Enrichment of intracellular proteins using a totally synthetic host-guest complex is described, overcoming the problem associated with the classical approach. We achieve this by affinity-based protein labeling with a target-specific probe molecule conjugated to a high-affinity guest (suberanilohydroxamic acid-ammonium-adamantane; SAHA-Ad) and then enriching the labeled species using a cucurbit[7]uril bead. This method shows high specificity for labeled molecules in a MDA-MB-231 breast cancer cell lysate. Moreover, this method shows promise for labeling proteins in live cells.
Center for Self-assembly and Complexity (CSC), Institute of Basic Science (IBS), Pohang, 37673, Republic of Korea.
James
J
Murray
School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, 37673, Republic of Korea.
Jaehwan
J
Sim
Division of Advanced Materials Science, Pohang University of Science and Technology, Pohang, 37673, Republic of Korea.
Kyunghoon
K
Oh
Division of Advanced Materials Science, Pohang University of Science and Technology, Pohang, 37673, Republic of Korea.
Gihyun
G
Sung
Division of Advanced Materials Science, Pohang University of Science and Technology, Pohang, 37673, Republic of Korea.
Ara
A
Lee
Center for Self-assembly and Complexity (CSC), Institute of Basic Science (IBS), Pohang, 37673, Republic of Korea.
Annadka
A
Shrinidhi
Center for Self-assembly and Complexity (CSC), Institute of Basic Science (IBS), Pohang, 37673, Republic of Korea.
Ayyavu
A
Thirunarayanan
Center for Self-assembly and Complexity (CSC), Institute of Basic Science (IBS), Pohang, 37673, Republic of Korea.
Dinesh
D
Shetty
Center for Self-assembly and Complexity (CSC), Institute of Basic Science (IBS), Pohang, 37673, Republic of Korea.|School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, 37673, Republic of Korea.|Department of Chemistry, Pohang University of Science and Technology, Pohang, 37673, Republic of Korea.|Division of Advanced Materials Science, Pohang University of Science and Technology, Pohang, 37673, Republic of Korea.
Kimoon
0000-0001-9418-3909
K
Kim
D000218:Adamantane / Q000737:chemistry*; D000345:Affinity Labels / Q000032:analysis / Q000302:isolation & purification; D064751:Ammonium Compounds / Q000737:chemistry*; D001952:Bridged-Ring Compounds / Q000737:chemistry*; D045744:Cell Line, Tumor; D006801:Humans; D007093:Imidazoles / Q000737:chemistry*; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods; D000077337:Vorinostat / Q000737:chemistry*; D047028:Macrocyclic Compounds; D000099246:Cucurbit(n)urils
D000218:Adamantane; D000345:Affinity Labels; D064751:Ammonium Compounds; D001952:Bridged-Ring Compounds; D007093:Imidazoles; D011506:Proteins; D000077337:Vorinostat; C456276:cucurbit(7)uril; D047028:Macrocyclic Compounds; D000099246:Cucurbit(n)urils
cucurbiturils; host-guest chemistry; protein chemistry; protein enrichment; supramolecular chemistry