Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin-Fixed and Paraffin-Embedded Tissue.
Autophagy is a highly conserved cellular mechanism of "self digestion," ensuring cellular homeostasis, and playing a role in many diseases including cancer. As a stress response mechanism, it may also be involved in cellular response to therapy.LC3 and Sequestosome 1 (p62/SQSTM1) are among the most widely used markers to monitor autophagy, and can be visualized in formalin-fixed and paraffin-embedded tissue by immunohistochemistry. Here we describe a validated staining protocol using an automated staining system available in many routine pathology laboratories, enabling high-throughput staining under standardized conditions.
Institute of Pathology, University of Bern, Murtenstrasse 31, 3010, Bern, Switzerland. sabina.berezowska@pathology.unibe.ch.
Sabina
S
Berezowska
Institute of Pathology, University of Bern, Murtenstrasse 31, 3010, Bern, Switzerland.
José A
JA
Galván
D001343:Autophagy; D015415:Biomarkers; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D009369:Neoplasms / Q000175:diagnosis / Q000378:metabolism; D016612:Paraffin Embedding; D000071456:Sequestosome-1 Protein / Q000378:metabolism*
D015415:Biomarkers; C477012:MAP1LC3A protein, human; D008869:Microtubule-Associated Proteins; C099718:SQSTM1 protein, human; D000071456:Sequestosome-1 Protein
Autophagy; Immunohistochemistry; LC3; Sequestosome 1; p62/SQSTM1