Analysis of codon usage patterns in Morus notabilis based on genome and transcriptome data.
Codons play important roles in regulating gene expression levels and mRNA half-lives. However, codon usage and related studies in multicellular organisms still lag far behind those in unicellular organisms. In this study, we describe for the first time genome-wide patterns of codon bias in Morus notabilis (mulberry tree), and analyze genome-wide codon usage in 12 other species within the order Rosales. The codon usage of M. notabilis was affected by nucleotide composition, mutation pressure, nature selection, and gene expression level. Translational selection optimal codons were identified and highly expressed genes of M. notabilis tended to use the optimal codons. Genes with higher expression levels have shorter coding region and lower amino acid complexity. Housekeeping genes showed stronger translational selection, which, notably, was not caused by the large differences between the expression level of housekeeping genes and other genes.
State Key Laboratory of Silkworm Genome Biology, Southwest University, Tiansheng Road, Beibei, Chongqing 400715, China.|State Key Laboratory of Silkworm Genome Biology, Southwest University, Tiansheng Road, Beibei, Chongqing 400715, China.
Yan
Y
Wen
State Key Laboratory of Silkworm Genome Biology, Southwest University, Tiansheng Road, Beibei, Chongqing 400715, China.|State Key Laboratory of Silkworm Genome Biology, Southwest University, Tiansheng Road, Beibei, Chongqing 400715, China.
Ziliang
Z
Zou
State Key Laboratory of Silkworm Genome Biology, Southwest University, Tiansheng Road, Beibei, Chongqing 400715, China.|State Key Laboratory of Silkworm Genome Biology, Southwest University, Tiansheng Road, Beibei, Chongqing 400715, China.
Hongshun
H
Li
State Key Laboratory of Silkworm Genome Biology, Southwest University, Tiansheng Road, Beibei, Chongqing 400715, China.|State Key Laboratory of Silkworm Genome Biology, Southwest University, Tiansheng Road, Beibei, Chongqing 400715, China.
Zhonghuai
Z
Xiang
State Key Laboratory of Silkworm Genome Biology, Southwest University, Tiansheng Road, Beibei, Chongqing 400715, China.|State Key Laboratory of Silkworm Genome Biology, Southwest University, Tiansheng Road, Beibei, Chongqing 400715, China.
Ningjia
N
He
D000596:Amino Acids / Q000235:genetics; D001482:Base Composition / Q000235:genetics; D003062:Codon / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics*; D031625:Morus / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D014176:Protein Biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012641:Selection, Genetic / Q000235:genetics; D059467:Transcriptome / Q000235:genetics*
D000596:Amino Acids; D003062:Codon; D012333:RNA, Messenger
Morus notabilis; codon usage; gène domestique; housekeeping gene; sélection transcriptionnelle; transcriptional selection; usage des codons