Human Papillomavirus types distribution among women with cervical preneoplastic, lesions and cancer in Luanda, Angola.
BACKGROUND
Cervical cancer is the leading cause of cancer deaths among females in Angola and human papillomavirus (HPV) is the main risk factor for the development of pre-cancerous squamous intraepithelial lesions. The diversity and frequency of HPV types in Angola has yet to be reported.


OBJECTIVE
To determine the frequency of HPV among women with squamous intraepithelial lesions from women in Luanda, Angola.


METHODS
Study participants included women diagnosed with cytological abnormalities that voluntarily provided Pap smears (n = 64). Genomic DNA was extracted from the samples for use as templates in the PCR amplification of HPV sequences. PCR products were sequenced to determine HPV type.


RESULTS
HPV DNA was detected in 71.9% (46/64) in the samples. A higher diversity of HPV types was found in the cytological lesions, such as ASCUS and LSIL (HPV16, 6, 18, 31, 58, 66, 70 and 82, in order of frequency) than that detected for HSIL and SSC (HPV16, 18, 6 and 33). The most prevalent HPV type were: HPV16, HPV6 and HPV18.


CONCLUSIONS
This is the first report on HPV type diversity and frequency in woman of Angola. The results suggest that large-scale studies across Africa would improve our understanding of interrelationship between HPV infections and cervical cancer. More directly, the identification of the HPV types most prevalent suggests that women in Angola would benefit from currently available HPV vaccines.
Departamento de Anatomia Patológica do Hospital Militar Principal, Instituto Superior, Luanda, Angola.
Paciência de Almeida
PA
Damião
Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil; Division of Genetic, National Institute of Cancer, Rio de Janeiro, Brazil.
Michelle
M
Oliveira-Silva
Division of Genetic, National Institute of Cancer, Rio de Janeiro, Brazil.
Miguel Ângelo
MÂ
Moreira
Departamento de Anatomia Patológica do Hospital Militar Principal, Instituto Superior, Luanda, Angola.
Natalia
N
Poliakova
Departamento de Anatomia Patológica do Hospital Militar Principal, Instituto Superior, Luanda, Angola.
Maria Emilia Rt
ME
de Lima
Departamento de Anatomia Patológica do Hospital Militar Principal, Instituto Superior, Luanda, Angola.
José
J
Chiovo
Laboratory of Interdisciplinary Medical Research, LIPMED, IOC, Fiocruz, Rio de Janeiro, Brazil.
Alcina Frederica
AF
Nicol
D000328:Adult; D000368:Aged; D000810:Angola / Q000453:epidemiology; D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000302:isolation & purification; D005260:Female; D006801:Humans; D008875:Middle Aged; D065006:Papanicolaou Test; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology; D016133:Polymerase Chain Reaction; D011230:Precancerous Conditions / Q000453:epidemiology / Q000821:virology; D012307:Risk Factors; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000821:virology*; D014626:Vaginal Smears; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000453:epidemiology / Q000821:virology*
D004279:DNA, Viral
Africa; Angola; HPV; cervical lesions