[Effects of ApoE gene polymorphism on the efficacy of atorvastatin in the treatment of hyperlipidemia].
Objective:To study the role of ApoE gene polymorphism on efficacy of atorvastatin in lowering the lipid and its clinical significance.Methods:A total of 962 patients with hypercholesterolemia were selected between January 1 st and December 31 st 2014. The ApoE genepolymorphism in patients with hyperlipidemia was performed by using polymerase chain reaction with restriction fragment length polymorphism (PCR-RFLP) method in translational medicine center of Huaihe Hospital. Patients with ApoE genotype E3/3 and E3/4 were selected and treated with atorvastatin 10 mg/d for 4 weeks. Before and after treatment, triglycerides (TG) and total cholesterol (TC) was detected by enzyme colorimetry method. High-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) were performed by Clearance method. Lipoprotein(a) (Lp(a)) was performed by turbidimetric inhibition immunoassay. ApoE gene expression was performed by real-time PCR.Results:In the 6 gene types, the frequencies of E3/4 and E3/3 were 30.6% (294 cases) and 59.1% (569 cases) respectively. After treatment with atorvastatin, the change percent of TC, LDL-C, HDL-C, TG, Lp(a) in E3/4 and E3/3 group were -(23.0±4.7)% vs -(12.0±3.1)% (P<0.001), -(33.0±4.8)% vs -(20.0±3.9)% (P<0.001), (18.0±3.8)% vs (6.0±2.6)% (P<0.001), -(23.0±3.9)% vs -(13.0±2.7)% (P<0.001), -(21.5±4.5)% vs -(20.9±4.0)% (P=0.054), respectively. ApoE gene expression in E3/3 and E3/4 groups were down-regulated in both groups, and the change in E3/3 group was obvious than that of E3/4 group.Conclusion:After treatment with atorvastatin, levels of lipids and ApoE gene expression in ApoE genotype E3/3 patients decreased, which were more evident than E3/4 patients.
Department of Nephrology, Huaihe Hospital, Henan University, Kaifeng 475000, China.
Y
Y
Zhang
D D
DD
Wei
R R
RR
Yuan
Q X
QX
Ge
F
F
Chen
S X
SX
Yang
B P
BP
Chen
J
J
Shi
D001057:Apolipoproteins E; D000069059:Atorvastatin; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D005838:Genotype; D006801:Humans; D006937:Hypercholesterolemia; D006949:Hyperlipidemias; D008055:Lipids; D017270:Lipoprotein(a); D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014280:Triglycerides
D001057:Apolipoproteins E; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D008055:Lipids; D017270:Lipoprotein(a); D014280:Triglycerides; D000069059:Atorvastatin
Apolipoproteins E; Atorvastatin; Hyperlipidemias; Polymorphism, restriction fragment length