Cattle naturally infected by Eurytrema coelomaticum: Relation between adenosine deaminase activity and zinc levels.
The enzyme adenosine deaminase (ADA) is critical for modulating the immune system, and in the presence of zinc, its activity is catalyzed. The aim of this study was to evaluate the ADA activity in pancreas of cattle naturally infected by Eurytrema coelomaticum in relation to the results of zinc levels, pathological findings and parasite load. For this study 51 slaughtered cattle were used. The animals were divided into two groups: Group A consisting of animals naturally infected by E. coelomaticum (n=33) and Group B of uninfected animals (n=18). Blood and pancreas were collected of each animal for analysis of zinc and ADA, respectively. Infected cattle showed a reduction on seric levels of zinc, and decreased ADA activity in the pancreas (P>0.05). A positive correlation between zinc levels and ADA activity was observed. Thus, high parasite load and severity of histopathologic lesions affect the ADA activity in pancreas, as well as the zinc levels in serum of infected animals (negative correlation between these variables). Therefore, we can conclude that cattle infected by E. coelomaticum have low ADA activity in pancreas, which can be directly related to zinc reduction, responsible for ADA activation and catalyzes.
Departamento of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil.
Hyolanda M
HM
Grosskopf
Veterinary School, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil.
Claiton I
CI
Schwertz
Veterinary School, UFGRS, Porto Alegre, RS, Brazil.
Gustavo
G
Machado
Department of Biochemistry and Molecular Biology, UFSM, Santa Maria, RS, Brazil.
Nathieli B
NB
Bottari
Veterinary School, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil.
Ester S
ES
da Silva
Veterinary School, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil.
Mateus E
ME
Gabriel
Veterinary School, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil.
Neuber J
NJ
Lucca
Department of Biochemistry and Molecular Biology, UFSM, Santa Maria, RS, Brazil.
Mariana S
MS
Alves
Department of Biochemistry and Molecular Biology, UFSM, Santa Maria, RS, Brazil.
Maria Rosa C
MR
Schetinger
Department of Biochemistry and Molecular Biology, UFSM, Santa Maria, RS, Brazil.
Vera M
VM
Morsch
Veterinary School, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil. Electronic address: ricardo.mendes@ifc-concordia.edu.br.
Ricardo E
RE
Mendes
Departamento of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil; Department of Biochemistry and Molecular Biology, UFSM, Santa Maria, RS, Brazil. Electronic address: aleksandro_ss@yahoo.com.br.
Aleksandro S
AS
da Silva
D000243:Adenosine Deaminase / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000201:enzymology / Q000378:metabolism* / Q000469:parasitology; D004012:Dicrocoeliidae / Q000502:physiology*; D010179:Pancreas / Q000378:metabolism / Q000469:parasitology*; D059208:Parasite Load / Q000662:veterinary*; D013053:Spectrophotometry / Q000662:veterinary; D014201:Trematode Infections / Q000201:enzymology / Q000378:metabolism / Q000469:parasitology / Q000662:veterinary*; D015032:Zinc / Q000097:blood*
D000243:Adenosine Deaminase; D015032:Zinc
ADA; Cattle; Mineral; Trematoda