Antioxidant and antigenotoxic potential of Ramaria largentii Marr & D. E. Stuntz, a wild edible mushroom collected from Northeast Romania.
Ramaria largentii Marr & D. E. Stuntz (orange coral mushroom) is a wild edible mushroom whose chemical composition and bioactivity have not been investigated. Herein, we present a study on the phenolic constituents, antioxidant and antigenotoxic effects of a hydromethanolic extract of the fruiting bodies. Total phenolic content, estimated by Folin-Ciocalteu assay, was found to be 42.33 ± 0.18 mg GAE/g. Protocatechuic and vanillic acids were detected by HPLC-DAD-ESI-MS. The extract showed good free radical scavenging and reducing capacities (EC50= 64.3 ± 0.2 and 61.54 ± 0.46 μg/mL, respectively). In normal Vero cells, the extract (100, 200 and 300 μg/mL) showed no genotoxic potential and moreover, almost completely protected DNA against H2O2-induced damage (2.09-7.91% tail DNA) (24 and 48 h pre-treatment). Taken together, the results of our study show that Ramaria largentii extract is devoid of genotoxicity and has a remarkable DNA protective activity against H2O2-induced damage.
Faculty of Pharmacy, Grigore T. Popa University of Medicine and Pharmacy Iasi, Universitatii Str. 16, 700115 Iasi, Romania. Electronic address: ana.aprotosoaie@umfiasi.ro.
Ana Clara
AC
Aprotosoaie
Faculty of Pharmacy, Grigore T. Popa University of Medicine and Pharmacy Iasi, Universitatii Str. 16, 700115 Iasi, Romania. Electronic address: daniela.zavastin@umfiasi.ro.
Daniela Elena
DE
Zavastin
Interdisciplinary Research Department-Field Science, Alexandru Ioan Cuza University of Iasi, Carol I Bd. 20A, 700506 Iasi, Romania; National Institute of Research and Development for Biological Sciences/Biological Research Institute, Lascar Catargi Str. 47, 700107 Iasi, Romania. Electronic address: cosmin.mihai@uaic.ro.
Cosmin-Teodor
CT
Mihai
National Institute of Research and Development for Biological Sciences/Biological Research Institute, Lascar Catargi Str. 47, 700107 Iasi, Romania. Electronic address: gabrielacapraru@yahoo.com.
Gabriela
G
Voichita
National Institute of Research and Development for Biological Sciences/Biological Research Institute, Lascar Catargi Str. 47, 700107 Iasi, Romania. Electronic address: daniela_gherghel@yahoo.com.
Daniela
D
Gherghel
Institute of Macromolecular Chemistry Petru Poni, Grigore Ghica Voda Al. 41A, 700487 Iasi, Romania. Electronic address: silion.mihaela@icmpp.ro.
Mihaela
M
Silion
Faculty of Pharmacy, Grigore T. Popa University of Medicine and Pharmacy Iasi, Universitatii Str. 16, 700115 Iasi, Romania. Electronic address: adriana.trifan@umfiasi.ro.
Adriana
A
Trifan
Faculty of Pharmacy, Grigore T. Popa University of Medicine and Pharmacy Iasi, Universitatii Str. 16, 700115 Iasi, Romania. Electronic address: ancamiron@yahoo.com.
Anca
A
Miron
D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001093:Arachidonate 15-Lipoxygenase / Q000378:metabolism; D002470:Cell Survival; D002614:Chelating Agents; D002522:Chlorocebus aethiops; D005504:Food Analysis; D007501:Iron; D016859:Lipoxygenase Inhibitors / Q000737:chemistry / Q000494:pharmacology; D009152:Mutagenicity Tests; D010084:Oxidation-Reduction; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology; D012383:Romania; D014709:Vero Cells
D000975:Antioxidants; D002614:Chelating Agents; D016859:Lipoxygenase Inhibitors; D010636:Phenols; D007501:Iron; D001093:Arachidonate 15-Lipoxygenase
Antioxidant activity; Genoprotection; Hydrogen peroxide; Mushrooms; Oxidative stress-induced DNA damage; Phenolics