Structure and specificity of a permissive bacterial C-prenyltransferase.
This study highlights the biochemical and structural characterization of the L-tryptophan C6 C-prenyltransferase (C-PT) PriB from Streptomyces sp. RM-5-8. PriB was found to be uniquely permissive to a diverse array of prenyl donors and acceptors including daptomycin. Two additional PTs also produced novel prenylated daptomycins with improved antibacterial activities over the parent drug.
Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, Kentucky, USA.|Center for Pharmaceutical Research and Innovation (CPRI), University of Kentucky, Lexington, Kentucky, USA.
Sherif I
SI
Elshahawi
Department of Biosciences, Rice University, Houston, Texas, USA.
Hongnan
H
Cao
Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, Kentucky, USA.|Center for Pharmaceutical Research and Innovation (CPRI), University of Kentucky, Lexington, Kentucky, USA.
Khaled A
KA
Shaaban
Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, Kentucky, USA.|Center for Pharmaceutical Research and Innovation (CPRI), University of Kentucky, Lexington, Kentucky, USA.
Larissa V
LV
Ponomareva
Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, Kentucky, USA.
Thangaiah
T
Subramanian
Department of Plant Pathology, University of Kentucky, Lexington, Kentucky, USA.
Mark L
ML
Farman
Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, Kentucky, USA.|Department of Chemistry, Markey Cancer Center, Kentucky Center for Structural Biology, University of Kentucky, Lexington, Kentucky, USA.
H Peter
HP
Spielmann
Department of Biosciences, Rice University, Houston, Texas, USA.
George N
GN
Phillips
Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, Kentucky, USA.|Center for Pharmaceutical Research and Innovation (CPRI), University of Kentucky, Lexington, Kentucky, USA.
Jon S
JS
Thorson
Center for Pharmaceutical Research and Innovation (CPRI), University of Kentucky, Lexington, Kentucky, USA.
Shanteri
S
Singh
D004122:Dimethylallyltranstransferase / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D015394:Molecular Structure; D013302:Streptomyces / Q000201:enzymology*; D013379:Substrate Specificity
D004122:Dimethylallyltranstransferase