Application of modified yeast surface display technologies for non-Antibody protein engineering.
Yeast surface display (YSD) system has been widely used in protein engineering since it was established 20 years ago. Combined with fluorescence-activated cell sorting (FACS) technology and directed evolution, YSD has been proven of its extraordinary effectiveness for molecular engineering of various target proteins, especially for antibodies. Recently, a few remarkable efforts were exploited to modify the original Aga1-Aga2 YSD for the non-antibody protein engineering with successful outcomes, expanding its application on oxidase, Class II major histocompatibility complex (MHC-II), protease, sortase, lipoic acid ligase etc. Here, the methodologies of these optimized Aga1-Aga2 YSD technologies were introduced, and the recent progress of non-antibody protein engineering using these methods was summarized.
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, 430062, China.
Meng
M
Mei
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, 430062, China.
Yu
Y
Zhou
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, 430062, China.
Wenfang
W
Peng
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, 430062, China.
Chan
C
Yu
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, 430062, China.
Lixin
L
Ma
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, 430062, China. Electronic address: zhangguimin6@hotmail.com.
Guimin
G
Zhang
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, 430062, China. Electronic address: liyi@hubu.edu.cn.
Li
L
Yi
D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D005434:Flow Cytometry / Q000379:methods; D015202:Protein Engineering / Q000379:methods*; D015003:Yeasts / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*
D000911:Antibodies, Monoclonal
Directed evolution; FACS; Non-antibody protein engineering; Yeast surface display