Osteogenic differentiation of human gingival mesenchymal stem cells by Aristolochia bracteolata supplementation through enhanced Runx2 expression.
The phenotypic characteristics of human gingival derived mesenchymal stem cells (HGMSCs) on induction with total methanol extract of Aristolochia bracteolata have been evaluated. HGMSCs were cultured in control and two different induction medium: Control medium (basal medium), OM1 (Standard induction medium), and OM2 (100 μg/ml of A. bracteolata). Osteogenic differentiation of the cultured cells was assessed by studying the calcium deposition and osteoblastic gene expression. OM2 medium showed an enhanced osteogenic differentiation potential than OM1 as measured by increased calcium deposition and elevated expression of Runx2, osteopontin, osteonectin, osteocalcin, Collagen type I, and ALP levels in comparison with OM1 differentiated cells. We conclude that at 100 μg/ml A. bracteolata has induced HGMSC differentiation into osteogenic lineage consequent to enhanced Runx2 expression and related osteogenic genes.
Centre for Indian Systems of Medicine Quality Assurance and Standardization Sri Ramachandra University, Chennai, India.
Dinesh
D
Murugan Girija
Department of Periodontology, Sri Ramachandra University, Chennai, India.
Suresh
S
Y Ranga Rao
Faculty of Pharmacy, Sri Ramachandra University, Chennai, India.
Mangathayaru
M
Kalachaveedu
Centre for Regenerative Medicine and Stem Cell Research, Sri Ramachandra University, Chennai, India.
Rajasekaran
R
Subbarayan
D000328:Adult; D030022:Aristolochia / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D019070:Cell Lineage / Q000187:drug effects / Q000235:genetics; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects / Q000235:genetics; D010936:Plant Extracts / Q000494:pharmacology; D055815:Young Adult
D050659:Core Binding Factor Alpha 1 Subunit; D010936:Plant Extracts
Aristolochia bracteolata; human gingival stem cells; osteogenic differentiation