Repair of bone defects with prefabricated vascularized bone grafts and double-labeled bone marrow-derived mesenchymal stem cells in a rat model.
This study aims to investigate the repair of bone defects with prefabricated vascularized bone grafts and double-labeled bone marrow-derived mesenchymal stem cells (BMSCs) in a rat model. BMSCs were separated from rat bone marrow. LTR-CMVpro-RFP and LTR-CMVpro-GFP were transfected into the BMSCs for in vitro and in vivo tracking. BMSCs-RFP and BMSCs-GFP were induced into endothelial progenitor cells (EPCs) and osteoblasts (OBs). Rats were divided into five groups: Group A: in vitro prefabrication with EPCs-RFP + in vivo prefabrication with arteriovenous vascular bundle + secondary OBs-GFP implantation; Group B: in vitro prefabrication with EPCs-RFP + secondary OBs-GFP implantation; Group C: in vivo prefabrication with arteriovenous vascular bundle + secondary OBs-GFP implantation; Group D: implantation of EPCs-RFP + implantation of with arteriovenous vascular bundle + simultaneous OBs-GFP implantation; Group E: demineralized bone matrix (DBM) grafts (blank control). Among five groups, Group A had the fastest bone regeneration and repair, and the regenerated bone highly resembled normal bone tissues; Group D also had fast bone repair, but the repair was slightly slower than Group A. Therefore, in vitro prefabrication with EPCs-RFP plus in vivo prefabrication with arteriovenous vascular bundle and secondary OBs-GFP implantation could be the best treatment for bone defect.
Department of Orthopedics, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai 264000, P.R. China.
Xiao-Rui
XR
Jiang
Department of Intensive Care Unit, Yantai Infectious Disease Hospital, Yantai 264000, P.R. China.
Hui-Ying
HY
Yang
Department of Orthopedics, Canner Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100000, P.R. China.
Xin-Xin
XX
Zhang
Department of Orthopedics, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai 264000, P.R. China.
Guo-Dong
GD
Lin
Department of Orthopedics, The Affiliated Yantai Hospital of Binzhou Medical University, Yantai 264000, P.R. China.
Yong-Chun
YC
Meng
Department of Trauma and Orthopedics, Peking University People's Hospital, Beijing 100000, P.R. China.
Pei-Xun
PX
Zhang
Southern Medical University, Guangzhou 510515, P.R. China.
Shan
S
Jiang
Binzhou Medical University, Yantai 264000, P.R. China.
Chun-Lei
CL
Zhang
Binzhou Medical University, Yantai 264000, P.R. China.
Fei
F
Huang
Department of Orthopedics, The Affiliated Yantai Hospital of Binzhou Medical University, Yantai 264000, P.R. China.
Lin
L
Xu
D000818:Animals; D001847:Bone Diseases / Q000628:therapy*; D016025:Bone Transplantation / Q000379:methods*; D017690:Cell Transplantation / Q000379:methods*; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051381:Rats; D013194:Staining and Labeling; D016896:Treatment Outcome
D008164:Luminescent Proteins