Purification, structural features, antioxidant and moisture-preserving activities of an exopolysaccharide from Lachnum YM262.
A water-soluble exopolysaccharide, designated as LEP-2a, was isolated from Lachnum YM262 and purified by DEAE-Cellulose 52 and Sepharose CL-6B chromatographic columns. LEP-2a was a homogeneous polysaccharide, with a molecular weight of 1.52×105Da. It was composed of mannose and galactose in a molar ratio of 20.6:1.0. Its structural features were investigated and elucidated by methylation analysis, periodate oxidation and Smith degradation, FT-IR and NMR spectroscopy. Based on obtained data, the backbone of LEP-2a consisted of 1,2-linked-α-d-mannose, 1,3-linked-α-d-mannose, 1,2,6-linked-α-d-mannose and 1,3-linked-β-d-galactose and the side chains were attached to the backbone at O-6 position of 1,2,6-linked-α-d-mannose. In vitro antioxidant activity assay proved that LEP-2a possessed significant scavenging activities on superoxide, hydroxyl and DPPH radical. Furthermore, LEP-2a had strong in vitro moisture-absorption and -retention capacities as compared to chitosan and glycerol. These results suggested that LEP-2a might have a good potential to be applied as a multifunctional cosmetic additive in cosmetics.
Microbial Resources and Application Laboratory, School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
Tianle
T
Chen
Microbial Resources and Application Laboratory, School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
Ping
P
Xu
Microbial Resources and Application Laboratory, School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
Shuai
S
Zong
Microbial Resources and Application Laboratory, School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
Yufen
Y
Wang
Microbial Resources and Application Laboratory, School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
Nana
N
Su
Microbial Resources and Application Laboratory, School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China. Electronic address: yeming123@sina.com.
Ming
M
Ye
D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001203:Ascomycota / Q000737:chemistry*; D001205:Ascorbic Acid / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010084:Oxidation-Reduction / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D011310:Preservatives, Pharmaceutical / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D012995:Solubility; D014867:Water / Q000737:chemistry*
D000975:Antioxidants; C000623091:LEP-2a compound; D011134:Polysaccharides; D011310:Preservatives, Pharmaceutical; D014867:Water; D001205:Ascorbic Acid
Antioxidant; Lachnum exopolysaccharide; Moisture absorption; Moisture retention; Structural features