A stretchable and screen-printed electrochemical sensor for glucose determination in human perspiration.
Here we present two types of all-printable, highly stretchable, and inexpensive devices based on platinum (Pt)-decorated graphite for glucose determination in physiological fluids. Said devices are: a non-enzymatic sensor and an enzymatic biosensor, the latter showing promising results. Glucose has been quantified by measuring hydrogen peroxide (H2O2) reduction by chronoamperometry at -0.35V (vs pseudo-Ag/AgCl) using glucose oxidase immobilized on Pt-decorated graphite. The sensor performs well for the quantification of glucose in phosphate buffer solution (0.25M PBS, pH 7.0), with a linear range between 0 mM and 0.9mM, high sensitivity and selectivity, and a low limit of detection (LOD). Thus, it provides an alternative non-invasive and on-body quantification of glucose levels in human perspiration. This biosensor has been successfully applied on real human perspiration samples and results also show a significant correlation between glucose concentration in perspiration and glucose concentration in blood measured by a commercial glucose meter.
Instituto Universitario de Materiales, Universidad de Alicante, Apartado 99, 03080 Alicante, Spain.
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Abellán-Llobregat
Department of NanoEngineering, University of California, La Jolla, San Diego, CA 92093, USA.
Itthipon
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Jeerapan
Department of NanoEngineering, University of California, La Jolla, San Diego, CA 92093, USA.
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Bandodkar
Departamento de Química Analítica, Nutrición y Bromatología and Instituto Universitario de Materiales, Universidad de Alicante, Apartado 99, 03080 Alicante, Spain.
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Vidal
Departamento de Química Analítica, Nutrición y Bromatología and Instituto Universitario de Materiales, Universidad de Alicante, Apartado 99, 03080 Alicante, Spain.
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Canals
Department of NanoEngineering, University of California, La Jolla, San Diego, CA 92093, USA.
J
J
Wang
Instituto Universitario de Materiales, Universidad de Alicante, Apartado 99, 03080 Alicante, Spain.
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Morallón
D000328:Adult; D015374:Biosensing Techniques / Q000295:instrumentation*; D004548:Elasticity; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D005260:Female; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis; D057230:Limit of Detection; D008297:Male; D010984:Platinum / Q000737:chemistry; D013542:Sweat / Q000737:chemistry*; D055815:Young Adult
D004800:Enzymes, Immobilized; D010984:Platinum; D006108:Graphite; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose
Catalyst; Glucose biosensor; H(2)O(2); Perspiration; Printed electronics; Stretchable device