Fluorogenic boronate-based probe-lactulose complex for full-aqueous analysis of peroxynitrite.
A selective fluorogenic boronate-based probe-lactulose complex was evaluated for the rapid analysis of peroxynitrite (ONOO-) based on a reaction-based indicator displacement assay (RIA). The probe was synthesised by a simple nucleophilic substitution reaction between a boronic acid moiety and a well known laser dye, DCM. Fluorescence analyses showed that the probe had an off-on response to lactulose, forming a fluorogenic probe-lactulose complex. The subsequent addition of ONOO-selectively quenched the fluorescence of the complex over other Reactive Oxygen/ Nitrogen Species (ROS/RNS) tested. The complex can be applied for the rapid determination of ONOO-in full aqueous solution with good linear range, and has also proven suitable for monitoring ONOO-in living cells and real water samples.
Department of Chemistry, Taizhou University, Jiaojiang 318000, PR China. Electronic address: lkbecust@126.com.
Kai-Bin
KB
Li
Institut de Chimie et Biochimie Moléculaires et Supramoléculaires,, Laboratoire de Chimie Organique 2 - Glycochimie, UMR 5246, Université Lyon 1 and CNRS, 43 Boulevard du 11 Novembre 1918, F-69622, Villeurbanne, France.
Lei
L
Dong
Department of Chemistry, Taizhou University, Jiaojiang 318000, PR China.
Siqi
S
Zhang
Department of Chemistry, Taizhou University, Jiaojiang 318000, PR China.
Wei
W
Shi
Department of Chemistry, Taizhou University, Jiaojiang 318000, PR China.
Wen-Ping
WP
Jia
Department of Chemistry, Taizhou University, Jiaojiang 318000, PR China. Electronic address: hdm@tzc.edu.cn.
De-Man
DM
Han
DCM; Fluorescent probe; Full aqueous; Lactulose; Peroxynitrite