Function annotation of peptides generated from the non-coding regions of D. melanogaster genome.
De novo emergence of genes is the most fundamental form of genetic diversity that is attracting the attention of the scientific community. Identification of short open reading frames (sORFs) from the non-coding regions of different genomes has been leading this thought recently. The coding potential of these newly identified sORFs have been investigated through experimental and computational approaches in recent studies. In the present work we have tried to make peptides from intergenic sequences of D. melanogaster genome leading to therapeutic applications. Towards this goal of making novel peptides from non-coding genome, we have found strong computational evidence of 145 peptides with conformational stability from the intergenic sequences of D. melanogaster. The structure of these completely unique peptides was predicted using ab initio method. The function annotation of these peptides was carried out using this structural information. The newly generated proteins were categorised as DNA/Protein/ion binding proteins, electron transporters and a very few as enzymes too. Experimental studies can certainly provide validations to these preliminary findings. This work provides further evidence of untapped potential of non-coding genome.
Department of Computational Biology and Bioinformatics, University of Kerala, Kariyavattom, Trivandrum.
Varughese
V
Deepthi
Indian Institute of Information Technology and Management, Kerala,Technopark, Trivandrum, India.
Vineetha
V
V I Nair
Department of Computational Biology and Bioinformatics, University of Kerala, Kariyavattom, Trivandrum.
Vipin
V
Thomas
Department of Computational Biology and Bioinformatics, University of Kerala, Kariyavattom, Trivandrum.
Navya
N
Raj
Department of Computational Biology and Bioinformatics, University of Kerala, Kariyavattom, Trivandrum.
Shidhi
S
P Ramakrishnan
School of Biotechnology,Jawaharlal Nehru University, New Delhi 110067.
Juveria
J
Khan
School of Biotechnology,Jawaharlal Nehru University, New Delhi 110067.
Monika
M
Kaushik
Department of Computational Biology and Bioinformatics, University of Kerala, Kariyavattom, Trivandrum;; School of Biotechnology,Jawaharlal Nehru University, New Delhi 110067.
Pawan
P
K Dhar
Department of Computational Biology and Bioinformatics, University of Kerala, Kariyavattom, Trivandrum.
Achuthsankar
A
S Nair
Non-coding; antimicrobial peptides; de novo peptides; junk DNA; short ORFs