Two-photon fluorescence sensors for imaging NMDA receptors and monitoring release of Zn2+from the presynaptic terminal.
Synaptic Zn2+plays an important role in neurotransmission and a neuromodulator. The development of the imaging tools for monitoring spatiotemporal changes taking place in synaptic Zn2+concentrations is necessary in order to understand the role of Zn2+in the function of many aspects of the glutamate system. In this work, two-photon probes 1 and 2, bearing ifenprodil-like tails that have affinity for NMDA receptors of neuronal cells, were designed and prepared. The two-photon fluorescent probe 1, which bears (N-(6-acetylnaphthalen-2-yl)-N-methylglycine) as two-photon fluorophore, enables high resolution imaging of neuronal cells. The two-photon fluorescent probe 2, which contains the di-2-picolylamine (DPA) as a Zn2+-binding site, the naphthalimide unit as the two-photon fluorophore, and the ifenprodil-like tail as the NMDA receptor binding moiety, can be employed for selective detection of Zn2+located near the NMDA receptor and for monitoring concentration changes of Zn2+in live neurons and hippocampal tissues.
Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Korea; State Key laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing 210009, China.
Xiaoqiang
X
Chen
Department of Energy Systems Research, Ajou University, Suwon 443-749, Korea.
Chang Su
CS
Lim
Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Korea.
Dayoung
D
Lee
Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Korea.
Songyi
S
Lee
Department of Energy Systems Research, Ajou University, Suwon 443-749, Korea.
Sang Jun
SJ
Park
Department of Energy Systems Research, Ajou University, Suwon 443-749, Korea. Electronic address: kimhm@ajou.ac.kr.
Hwan Myung
HM
Kim
Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Korea. Electronic address: jyoon@ewha.ac.kr.
Juyoung
J
Yoon
D000588:Amines / Q000737:chemistry*; D000818:Animals; D002413:Cations, Divalent / Q000032:analysis / Q000378:metabolism; D002478:Cells, Cultured; D005456:Fluorescent Dyes / Q000737:chemistry*; D006624:Hippocampus / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D009474:Neurons / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D061848:Optical Imaging / Q000379:methods; D010848:Picolinic Acids / Q000737:chemistry*; D017729:Presynaptic Terminals / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000032:analysis* / Q000378:metabolism; D012521:Sarcosine / Q000737:chemistry*; D015032:Zinc / Q000032:analysis* / Q000378:metabolism
C501847:2,2'-dipicolylamine; D000588:Amines; D002413:Cations, Divalent; D005456:Fluorescent Dyes; D010848:Picolinic Acids; D016194:Receptors, N-Methyl-D-Aspartate; D015032:Zinc; D012521:Sarcosine
Fluorescent probes; NMDA receptors; Presynaptic terminal; Two-photon imaging; Zinc ions