Cytotoxic effect of ferrimagnetic glass-ceramic nanocomposites on bone osteosarcoma cells.
This work pointed out the anti-cancer effect of ferrimagnetic glass ceramic nanocomposites (CaO-ZnO-Fe2O3-SiO2),which contain high amount of magnetite (∼60%), crystallite size <100nm, and different nucleating agents on bone cancer Saos-2 cells. The cell viability was inhibited by FH and FW to <50% and <25%, respectively, with/without magnetism, and both also reduced mitochondrial transmembrane potential (ΔYm), with/without magnetism (no influence of magnetism). Histone deacetylase (HDAC) activity was inhibited by FH, FW, and FHPNT, with/without magnetism. FHP3/magnetism resulted in HDAC inhibition. In absence of magnetism, FH and FW increased both necrotic and apoptotic cell death, while FW/magnetism induced late apoptosis. DNA fragmentation was increased by FH- and FW-treatment, with/without magnetism. At the same time, FW and FH/magnetism can efficiently induce the intrinsic apoptotic pathway in Saos-2 cells, whereas FW with/without magnetism and FH/magnetism enhanced cytochrome-C release. Similarly, caspase-7 activity was elevated by FH and FW, with/without magnetism. However, the presence of P2O5in the composition of the nanocomposites inhibited their apoptotic properties and diminished their anti-cancer activity.
Cancer Biology and Genetics Laboratory, Centre of Excellence for Advanced Sciences, National Research Centre, Dokki, 12622, Cairo, Egypt; Biochemistry Department, National Research Centre, Dokki, Cairo, Egypt. Electronic address: aeldeen7@yahoo.com.
Amira M
AM
Gamal-Eldeen
Glass Research Department, National Research Centre, Dokki, Cairo, Egypt.
Salwa A M
SAM
Abdel-Hameed
Cancer Biology and Genetics Laboratory, Centre of Excellence for Advanced Sciences, National Research Centre, Dokki, 12622, Cairo, Egypt; Department of Medical Biochemistry, National Research Centre, Cairo, Egypt.
Sherien M
SM
El-Daly
Cancer Biology and Genetics Laboratory, Centre of Excellence for Advanced Sciences, National Research Centre, Dokki, 12622, Cairo, Egypt; Genetics and Cytology Department, National Research Centre, Cairo, Egypt.
Mona A M
MAM
Abo-Zeid
Biochemistry Department, National Research Centre, Dokki, Cairo, Egypt.
Menha M
MM
Swellam
D017209:Apoptosis / Q000187:drug effects; D001859:Bone Neoplasms / Q000188:drug therapy*; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002516:Ceramics / Q000494:pharmacology*; D053938:DNA Fragmentation / Q000187:drug effects; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006655:Histone Deacetylases / Q000378:metabolism; D006801:Humans; D059346:Magnets; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D053761:Nanocomposites; D012516:Osteosarcoma / Q000188:drug therapy*
D056572:Histone Deacetylase Inhibitors; C023506:Glass ceramics; D006655:Histone Deacetylases
Apoptosis; Cancer; Ferrimagnetic; Glass-ceramic; Hyperthermia; Magnetite