An optimized transurethral catheterization cystometry in mice and comparison with classic suprapubic catheterization cystometry.
OBJECTIVE
The aim of this study is to establish an optimized, minimally invasive transurethral catheterization cystometry (TUCC) and a novel urethral pressure profile (UPP) measurement for mice.


METHODS
The optimized TUCC and the UPP measurement were first established. This optimized TUCC was then performed in 16 anesthetized female mice and compared with the suprapubic catheterization cystometry (SCC) in parallel after suprapubic catheters implantation (SCI; on zero, third, and seventh day, respectively). Finally, the optimized TUCC and novel UPP measurement were applied to investigate in another eight mice of partial bladder outlet obstruction (pBOO) model. The urodynamic parameters including micturition pressure (MP), basal pressure (BP), threshold pressure (TP), bladder capacity (BC), micturition volume (MV), residual urine (RV), bladder compliance (COM), maximum urethral pressure (MUP), bladder pressure curve and UPP were recorded. Statistical cross-comparisons of parameters for two kinds of cystometries and pBOO model were performed.


RESULTS
Compared with the optimized TUCC before SCI, the MV, RV, BC, and MP decreased significantly on the seventh day after SCI (270.4-132.5 µL, 46.13-20.09 µL, 316.4-152.5 µL, 30.01-24.34 cmH20, respectively). After SCI, the BP, MP, TP, MV, RV, BC, and COM showed no significant difference between the TUCC and SCC at the same time point. The MUP increased significantly after pBOO operation (19.1-46.6 cmH20, P < 0.05).


CONCLUSIONS
The minimally invasive TUCC along with UPP measurement could be widely applied to study the bladder function of mice as a feasible, repeatable, and accessible method.
Department of Urology, Zhongshan Hospital, Fudan University, Shanghai, China.
Zhuoyi
Z
Xiang
Department of Urology, Zhongshan Hospital, Fudan University, Shanghai, China.
Yaohui
Y
Li
Department of Urology, Zhongshan Hospital, Fudan University, Shanghai, China.
Tingchang
T
Bian
Department of Urology, Zhongshan Hospital, Fudan University, Shanghai, China.
Minke
M
He
Department of Urology, Zhongshan Hospital, Fudan University, Shanghai, China.
Yeqing
Y
Xu
Department of Urology, Zhongshan Hospital, Fudan University, Shanghai, China.
Guomin
G
Wang
Department of Urology, Zhongshan Hospital, Fudan University, Shanghai, China.
Jianming
J
Guo
Department of Urology, Zhongshan Hospital, Fudan University, Shanghai, China.
Hang
H
Wang
D000818:Animals; D005260:Female; D051379:Mice; D001748:Urinary Bladder Neck Obstruction / Q000503:physiopathology*; D014546:Urinary Catheterization / Q000379:methods*; D014554:Urination / Q000502:physiology*; D014563:Urodynamics / Q000502:physiology*
comparison; mice; suprapubic catheterization cystometry; transurethral catheterization cystometry; urethral pressure profile