Flow-Cytometry-Based Method to Detect Escherichia coli and Shigella Spp. Using 16S rRNA-Based Probe.
Detection of microbial contamination in foods before they go on to the market can help prevent the occurrence of foodborne illness outbreaks. Current methods for the detection of Escherichia coli are limited by time-consuming procedures, which include multiple culture incubation steps, and require several days to get results. This unit describes the development of an improved rapid flow-cytometry-based detection method that has greater sensitivity and specificity. This method requires less time-to-results (TTR) and can detect a small number of E. coli in the presence of large numbers of other bacteria. Clear step-by-step protocols for cell concentration determination, sample preparation, and flow cytometric analysis are provided. © 2017 by John Wiley & Sons, Inc.
Division of Systems Biology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, Arkansas.
Yong
Y
Xue
Division of Systems Biology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, Arkansas.
Jon G
JG
Wilkes
Life Sciences Consultant, Jonesboro, Arkansas.
Ted J
TJ
Moskal
Division of Systems Biology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, Arkansas.
Anna J
AJ
Williams
Division of Systems Biology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, Arkansas.
Willie M
WM
Cooper
Division of Systems Biology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, Arkansas.
Dan A
DA
Buzatu
D015169:Colony Count, Microbial; D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D005434:Flow Cytometry / Q000379:methods*; D005516:Food Microbiology; D057230:Limit of Detection; D015347:RNA Probes; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012760:Shigella / Q000235:genetics / Q000302:isolation & purification*
D003470:Culture Media; D015347:RNA Probes; D012336:RNA, Ribosomal, 16S
Escherichia coli; Salmonella; Shigella; flow cytometry; foodborne illness; microbial contamination; oligonucleotide probe