Air assisted emulsification liquid-liquid microextraction based on deep eutectic solvent for preconcentration of methadone in water and biological samples.
Nowadays, a deep eutectic solvent (DES) has recently been considered as a green ion liquid analogue. In this study, a new compound of DES has been synthesized as an extraction solvent in air assistedemulsification liquid-liquid microextraction method (DES-AAELLME) for preconcentration and extraction of methadone followed by gas chromatographyflame ionization detector (GC-FID). To obtain an efficient water-miscible deep eutectic solvent, choline chloride (ch-cl) and 5,6,7,8-Tetrahydro-5,5,8,8-tetramethylnaphthalen-2-ol (TNO) were mixed at a molar ratio of 1:2 and tetrahydrofuran (THF) was used as a demulsifier solvent into homogeneous solution for providing a turbid state. The solution was rapidly sucked up and injected 10 times using a 10-mL glass syringe to enhance the turbidity of solution and disperse the aggregated DES droplets into aqueous phase. Some important parameters affecting extraction recovery were investigated. Under optimum conditions, the calibration curve was linear in the concentration range from 2 to 8000µgL-1. The limit of detection and the limit of quantification were found 0.7µgL-1and 2.3µgL-1respectively with preconcentration factor of 270. The precision, as the relative standard deviation (RSD) (n=6), was <6% respectively. This method was successfully applied to determine methadone in water and biological samples with an appropriate recovery about 98.4-101.2%.
Drug Design and Development Research Center, Tehran University of Medical Sciences, Tehran, Iran.
N
N
Lamei
Department of Chemistry, Payame Noor University, P.O. BOX 19395-3697, Tehran, Iran.
M
M
Ezoddin
Drug Design and Development Research Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Medicinal Chemistry and Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: khmabdi@gmail.com.
K
K
Abdi
D000388:Air; D000701:Analgesics, Opioid / Q000032:analysis / Q000302:isolation & purification; D002138:Calibration; D006801:Humans; D057230:Limit of Detection; D059627:Liquid Phase Microextraction / Q000379:methods*; D008691:Methadone / Q000032:analysis* / Q000302:isolation & purification*; D012997:Solvents; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000302:isolation & purification
D000701:Analgesics, Opioid; D012997:Solvents; D014874:Water Pollutants, Chemical; D008691:Methadone
Air assisted emulsification liquid–liquid microextraction; Biological samples; Deep eutectic solvent; Methadone