Student award for outstanding research winner in the Ph.D. category for the 2017 society for biomaterials annual meeting and exposition, april 5-8, 2017, Minneapolis, Minnesota: Characterization of protein interactions with molecularly imprinted hydrogels that possess engineered affinity for high isoelectric point biomarkers.
Molecularly imprinted polymers (MIPs) with selective affinity for protein biomarkers could find extensive utility as environmentally robust, cost-efficient biomaterials for diagnostic and therapeutic applications. In order to develop recognitive, synthetic biomaterials for prohibitively expensive protein biomarkers, we have developed a molecular imprinting technique that utilizes structurally similar, analogue proteins. Hydrogel microparticles synthesized by molecular imprinting with trypsin, lysozyme, and cytochrome c possessed an increased affinity for alternate high isoelectric point biomarkers both in isolation and plasma-mimicking adsorption conditions. Imprinted and non-imprinted P(MAA-co-AAm-co-DEAEMA) microgels containing PMAO-PEGMA functionalized polycaprolactone nanoparticles were net-anionic, polydisperse, and irregularly shaped. MIPs and control non-imprinted polymers (NIPs) exhibited regions of Freundlich and BET isotherm adsorption behavior in a range of non-competitive protein solutions, where MIPs exhibited enhanced adsorption capacity in the Freundlich isotherm regions. In a competitive condition, imprinting with analogue templates (trypsin, lysozyme) increased the adsorption capacity of microgels for cytochrome c by 162% and 219%, respectively, as compared to a 122% increase provided by traditional bulk imprinting with cytochrome c. Our results suggest that molecular imprinting with analogue protein templates is a viable synthetic strategy for enhancing hydrogel-biomarker affinity and promoting specific protein adsorption behavior in biological fluids. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1565-1574, 2017.
Department of Biomedical Engineering, The University of Texas at Austin, Austin, Texas.|Institute for Biomaterials Drug Delivery and Regenerative Medicine, The University of Texas at Austin, Austin, Texas.
John R
JR
Clegg
McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, Texas.
Justin X
JX
Zhong
Department of Biomedical Engineering, The University of Texas at Austin, Austin, Texas.
Afshan S
AS
Irani
McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, Texas.
Joann
J
Gu
Institute for Biomaterials Drug Delivery and Regenerative Medicine, The University of Texas at Austin, Austin, Texas.|McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, Texas.
David S
DS
Spencer
Department of Biomedical Engineering, The University of Texas at Austin, Austin, Texas.|Institute for Biomaterials Drug Delivery and Regenerative Medicine, The University of Texas at Austin, Austin, Texas.|McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, Texas.|Division of Pharmaceutics, College of Pharmacy, The University of Texas at Austin, Austin, Texas.|Department of Surgery and Perioperative Care, Dell Medical School, The University of Texas at Austin, Austin, Texas.
Nicholas A
NA
Peppas
D000327:Adsorption; D000818:Animals; D001363:Awards and Prizes; D001672:Biocompatible Materials / Q000737:chemistry; D015415:Biomarkers / Q000032:analysis; D002417:Cattle; D002645:Chickens; D020100:Hydrogels / Q000737:chemistry*; D007526:Isoelectric Point; D054802:Molecular Imprinting / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D049388:Polyanhydrides / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification*; D013334:Students
D001672:Biocompatible Materials; D015415:Biomarkers; D020100:Hydrogels; D049388:Polyanhydrides; D011091:Polyesters; D011108:Polymers; D011506:Proteins; C450003:poly(ethylene glycol) methacrylate polymer; C016240:polycaprolactone; D011092:Polyethylene Glycols
biomimetics; intelligent hydrogels; molecular imprinting; protein adsorption