DNA target recognition domains in the Type I restriction and modification systems of Staphylococcus aureus.
Staphylococcus aureus displays a clonal population structure in which horizontal gene transfer between different lineages is extremely rare. This is due, in part, to the presence of a Type I DNA restriction-modification (RM) system given the generic name of Sau1, which maintains different patterns of methylation on specific target sequences on the genomes of different lineages. We have determined the target sequences recognized by the Sau1 Type I RM systems present in a wide range of the most prevalent S. aureus lineages and assigned the sequences recognized to particular target recognition domains within the RM enzymes. We used a range of biochemical assays on purified enzymes and single molecule real-time sequencing on genomic DNA to determine these target sequences and their patterns of methylation. Knowledge of the main target sequences for Sau1 will facilitate the synthesis of new vectors for transformation of the most prevalent lineages of this 'untransformable' bacterium.
EaStCHEM School of Chemistry, University of Edinburgh, The King's Buildings, Edinburgh, EH9 3FJ, UK.
Laurie P
LP
Cooper
EaStCHEM School of Chemistry, University of Edinburgh, The King's Buildings, Edinburgh, EH9 3FJ, UK.
Gareth A
GA
Roberts
EaStCHEM School of Chemistry, University of Edinburgh, The King's Buildings, Edinburgh, EH9 3FJ, UK.
John H
JH
White
New England Biolabs, 240 County Road, Ipswich, MA 01938-2723, USA.
Yvette A
YA
Luyten
EaStCHEM School of Chemistry, University of Edinburgh, The King's Buildings, Edinburgh, EH9 3FJ, UK.
Edward K M
EKM
Bower
New England Biolabs, 240 County Road, Ipswich, MA 01938-2723, USA.
Richard D
RD
Morgan
New England Biolabs, 240 County Road, Ipswich, MA 01938-2723, USA.
Richard J
RJ
Roberts
Institute of Infection and Immunity, St George's, University of London, Cranmer Terrace, London, SW17 0RE, UK.
Jodi A
JA
Lindsay
Department of Biosciences, Durham University, Stockton Road, Durham, DH1 3LE, UK.
David T F
DTF
Dryden
D000595:Amino Acid Sequence; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D015254:DNA Modification Methylases / Q000737:chemistry* / Q000378:metabolism*; D015253:Deoxyribonucleases, Type I Site-Specific / Q000737:chemistry* / Q000378:metabolism*; D000072417:Protein Domains; D017422:Sequence Analysis, DNA; D013211:Staphylococcus aureus / Q000201:enzymology* / Q000235:genetics; D014169:Transformation, Bacterial
D004247:DNA; D015254:DNA Modification Methylases; D015253:Deoxyribonucleases, Type I Site-Specific