Acute upregulation of PGC-1α mRNA correlates with training-induced increases in SDH activity in human skeletal muscle.
The purpose of the present study was to determine if acute responses in PGC-1α, VEGFA, SDHA, and GPD1-2 mRNA expression predict their associated chronic skeletal muscle molecular (SDH-GPD activity and substrate storage) and morphological (fibre-type composition and capillary density) adaptations following training. Skeletal muscle biopsies were collected from 14 recreationally active men (age: 22.0 ± 2.4 years) before (PRE) and 3 h after (3HR) the completion of an acute bout of sprint interval training (SIT) (eight 20-s intervals at ∼170% peak oxygen uptake work rate separated by 10 s of recovery). Participants then completed 6 weeks of SIT 4 times per week with additional biopsies after 2 (MID) and 6 (POST) weeks of training. Acute increases in PGC-1α mRNA strongly predicted increases in SDH activity (a marker of oxidative capacity) from PRE and MID to POST (PRE-POST: r = 0.81, r2= 0.65, p < 0.01; MID-POST: r = 0.79, r2= 0.62, p < 0.01) and glycogen content from MID to POST (r = 0.60, r2= 0.36, p < 0.05). No other significant relationships were found between acute responses in PGC-1α, VEGFA, SDHA, and GPD1-2 mRNA expression and chronic adaptations to training. These results suggest that acute upregulation of PGC-1α mRNA relates to the magnitude of subsequent training-induced increases in oxidative capacity, but not other molecular and morphological chronic skeletal muscle adaptations. Additionally, acute mRNA responses in PGC-1α correlated with VEGFA, but not SDHA, suggesting a coordinated upregulation between PGC-1α and only some of its proposed targets in human skeletal muscle.
a School of Kinesiology and Health Studies, Queen's University, Kingston, ON K7L 3N6 Canada.
Jacob T
JT
Bonafiglia
a School of Kinesiology and Health Studies, Queen's University, Kingston, ON K7L 3N6 Canada.
Brittany A
BA
Edgett
c Department of Kinesiology, University of Waterloo, Waterloo, ON N2L 3G1, Canada.
Brittany L
BL
Baechler
a School of Kinesiology and Health Studies, Queen's University, Kingston, ON K7L 3N6 Canada.
Matthew W
MW
Nelms
b Department of Emergency Medicine, Queen's University, Kingston, ON K7L 3N6, Canada.
Craig A
CA
Simpson
c Department of Kinesiology, University of Waterloo, Waterloo, ON N2L 3G1, Canada.
Joe
J
Quadrilatero
a School of Kinesiology and Health Studies, Queen's University, Kingston, ON K7L 3N6 Canada.
Brendon J
BJ
Gurd
D000222:Adaptation, Physiological / Q000235:genetics; D000328:Adult; D015444:Exercise; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006801:Humans; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D010101:Oxygen Consumption; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
C486497:PPARGC1A protein, human; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D012333:RNA, Messenger; D014280:Triglycerides; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D006003:Glycogen; D005993:Glycerolphosphate Dehydrogenase; D013385:Succinate Dehydrogenase
SDH activity; VEGFA; activité de la SDH; capillary density; densité capillaire; entraînement par intervalle au sprint; individual responses; réponses individuelles; sprint interval training