Purification, characterization, and antitumor activity of a novel glucan from the fruiting bodies of Coriolus Versicolor.
Cancer is one of the most common causes of deaths worldwide. Herein, we report an efficient natural anticancer glucan (CVG) extracted from Coriolus Versicolar (CV). CVG was extracted by the hot water extraction method followed by ethanol precipitation and purified using gas exclusion chromatography. Structural analysis revealed that CVG has a linear α-glucan chain composed of only (1→ 6)-α-D-Glcp. The antitumor activity of CVG on Sarcoma-180 cells was investigated in vitro and in vivo. Mice were treated with three doses of CVG (40, 100, 200 mg/kg body weight) for 9 days. Tumor weight, relative spleen, thymus weight, and lymphocyte proliferation were studied. A significant increase (P< 0.01) in relative spleen and thymus weight and a decrease (P< 0.01) in tumor weight at the doses of 100 and 200 mg/kg were observed. The results obtained demonstrate CVG has antitumor activity towards Sarcoma-180 cells by its immunomodulation activity.
Department of Biotechnology, Dalian Medical University, Dalian, P.R. China.|Department of Biochemistry and Molecular Biology, Northeast Normal University, Changchun, P.R. China.|Department of Biochemistry & Food Sciences, University of Kordofan, El-Obeid, The Republic of Sudan.
Annoor
A
Awadasseid
Department of Biotechnology, Dalian Medical University, Dalian, P.R. China.
Jie
J
Hou
Department of Biotechnology, Dalian Medical University, Dalian, P.R. China.
Yaser
Y
Gamallat
Department of Biotechnology, Dalian Medical University, Dalian, P.R. China.
Shang
S
Xueqi
Department of Biotechnology, Dalian Medical University, Dalian, P.R. China.
Kuugbee D
KD
Eugene
Department of pathology and pathophysiology, Dalian Medical University, Dalian, P.R. China.
Ahmed
A
Musa Hago
Department of Biotechnology, Dalian Medical University, Dalian, P.R. China.
Djibril
D
Bamba
Department of Biotechnology, Dalian Medical University, Dalian, P.R. China.
Abdo
A
Meyiah
Department of Biotechnology, Dalian Medical University, Dalian, P.R. China.
Chiwala
C
Gift
Department of Biotechnology, Dalian Medical University, Dalian, P.R. China.
Yi
Y
Xin
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001487:Basidiomycota / Q000737:chemistry*; D001688:Biological Products / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D005936:Glucans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D017550:Spectroscopy, Fourier Transform Infrared; D023041:Xenograft Model Antitumor Assays
D000970:Antineoplastic Agents; D001688:Biological Products; D005936:Glucans