Effect of vitamin D on isoprenaline-induced myocardial infarction in rats: possible role of peroxisome proliferator-activated receptor-γ.
Infarct-like lesion induced by isoprenaline is a well-known model to study myocardial infarction (MI). Vitamin D has been shown to have anti-inflammatory and antioxidant effects. Recent studies highlighted cross talk between vitamin D and peroxisome proliferator-activated receptor gamma (PPAR-γ). The present study was designed to investigate the effect of pretreatment with vitamin D on the isoprenaline-induced infarct-like lesion in rats and the role of PPAR-γ as a novel mechanism in vitamin-D-mediated cardioprotective effect. Markers chosen to assess cardiac damage included serum level of creatine kinase (CK), lactate dehydrogenase (LDH), tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6). Cardiac contents of malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH) were also assessed. Furthermore, ECG monitoring and measurement of injury extension were carried out. Isoprenaline increased the level of cardiac enzymes, as well as inflammatory and oxidative stress biomarkers. In addition, it produced ST-segment elevation. Pretreatment with vitamin D significantly improved previous parameters. The prior treatment with bisphenol A diglycidyl ether (BADGE), a PPAR-γ antagonist, significantly attenuated the protective effect of vitamin D. In conclusion, vitamin D can be demonstrated as a promising cardioprotective agent in MI and PPAR-γ significantly contributes toward vitamin-D-mediated protection.
Physiology Department, Faculty of Medicine, Benha University, Egypt.|Physiology Department, Faculty of Medicine, Benha University, Egypt.
Ola Ahmed
OA
El-Gohary
Physiology Department, Faculty of Medicine, Benha University, Egypt.|Physiology Department, Faculty of Medicine, Benha University, Egypt.
Mona Maher
MM
Allam
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004562:Electrocardiography; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D009203:Myocardial Infarction / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism* / Q000503:physiopathology; D018384:Oxidative Stress / Q000187:drug effects; D047495:PPAR gamma / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014807:Vitamin D / Q000494:pharmacology* / Q000627:therapeutic use
D015415:Biomarkers; D047495:PPAR gamma; D014807:Vitamin D; D007545:Isoproterenol
infarctus du myocarde; isoprenaline; isoprénaline; myocardial infarction; oxidative stress; peroxisome proliferator activated receptor-γ; récepteur γ activé par les proliférateurs de peroxysome; stress oxydatif; vitamin D; vitamine D