Veronicastrum axillareAlleviates Ethanol-Induced Injury on Gastric Epithelial Cells via Downregulation of the NF-kB Signaling Pathway.
We used human gastric epithelial cells (GES-1) line in an ethanol-induced cell damage model to study the protective effect ofVeronicastrum axillareand its modulation to NF-κB signal pathway. The goal was to probe the molecular mechanism ofV. axillaredecoction in the prevention of gastric ulcer and therefore provide guidance in the clinical application ofV. axillareon treating injuries from chronic nephritis, pleural effusion, gastric ulcer, and other ailments. The effects ofV. axillare-loaded serums on cell viability were detected by MTT assays. Enzyme-linked immunosorbent assay (ELISA) and Real-Time PCR methods were used to analyze the protein and mRNA expression of TNF-α, NF-κB, IκBα, and IKKβ. The results showed thatV. axillare-loaded serum partially reversed the damaging effects of ethanol and NF-κB activator (phorbol-12-myristate-13-acetate: PMA) and increased cell viability. The protein and mRNA expressions of TNF-α, NF-κB, IκBα, and IKKβwere significantly upregulated by ethanol and PMA while they were downregulated byV. axillare-loaded serum. In summary,V. axillare-loaded serum has significantly protective effect on GES-1 against ethanol-induced injury. The protective effect was likely linked to downregulation of TNF-αbased NF-κB signal pathway.
College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Wei-Chun
0000-0001-7264-3978
WC
Zhao
College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Yan-Shan
YS
Xu
Department of Chemistry, Simon Fraser University, Burnaby, BC, Canada V5A 1S6.
Gang
G
Chen
College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Yan
Y
Guo
College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Dan-Yi
DY
Wang
College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Gui-Bin
GB
Meng