Near infrared fluorescent imaging of choline kinase alpha expression and inhibition in breast tumors.
Choline kinase alpha (ChoKα) overexpression is associated with an aggressive tumor phenotype. ChoKα inhibitors induce apoptosis in tumors, however validation of their specificity is difficult in vivo. We report the use of optical imaging to assess ChoKα status in cells and in vivo using JAS239, a carbocyanine-based ChoKα inhibitor with inherent near infrared fluorescence. JAS239 attenuated choline phosphorylation and viability in a panel of human breast cancer cell lines. Antibody blockade prevented cellular retention of JAS239 indicating direct interaction with ChoKα independent of the choline transporters and catabolic choline pathways. In mice bearing orthotopic MCF7 breast xenografts, optical imaging with JAS239 distinguished tumors overexpressing ChoKα from their empty vector counterparts and delineated tumor margins. Pharmacological inhibition of ChoK by the established inhibitor MN58b led to a growth inhibition in 4175-Luc+ tumors that was accompanied by concomitant reduction in JAS239 uptake and decreased total choline metabolite levels as measured using magnetic resonance spectroscopy. At higher therapeutic doses, JAS239 was as effective as MN58b at arresting tumor growth and inducing apoptosis in MDA-MB-231 tumors, significantly reducing tumor choline below baseline levels without observable systemic toxicity. These data introduce a new method to monitor therapeutically effective inhibitors of choline metabolism in breast cancer using a small molecule companion diagnostic.
Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States.
Sean P
SP
Arlauckas
Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States.
Manoj
M
Kumar
Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States.
Anatoliy V
AV
Popov
Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States.|Department of Cellular and Molecular Physiology, Institute of Regenerative Medicine, University of Liverpool, Liverpool, United Kingdom.
Harish
H
Poptani
Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States.
Edward J
EJ
Delikatny
D000818:Animals; D001943:Breast Neoplasms / Q000201:enzymology* / Q000473:pathology; D045744:Cell Line, Tumor; D002797:Choline Kinase / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D015331:Cohort Studies; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D008819:Mice, Nude; D019265:Spectroscopy, Near-Infrared; D023041:Xenograft Model Antitumor Assays
C517212:CHKA protein, human; D002797:Choline Kinase
breast cancer; chemotherapy; choline kinase; fluorescence optical imaging