Multi metal assessment on biofilm formation in offshore environment.
Eight metal and non-metal coupons were exposed to seawater in offshore platform laboratory on biofilm formation in seven days of every month interval time for one year period. Among metal coupons, the highest mean heterotrophic biofilm bacteria population (HBBP) ranges were observed in mild steel (MS) coupon (33.03 to 40.00 [×104] CFUscm-2) and lowest in copper (Cu) coupon (3.0 to 5.1 [×104] CFUscm-2). Non-metal coupons show, highest mean HBBP ranges in glass (GL) coupon (29.6 to 66.0 [×104] CFUscm-2) and lowest in polyvinyl chloride (PVC) coupon (34.4 to 52.5 [×104] CFUscm-2). Within the studied coupons, high HBBP level was witnessed in GL and simultaneously low in Cu due to its toxic leaching product of Cu2O. Gram-positive bacterial deposit show dominated trend in all coupons with increasing order of Cu>SS>MS>PVC>GL>Cu-Ni 70/30>Cu-Ni 90/10>Br and Gram-negative show different in the order of Br>Cu-Ni 90/10>Cu-Ni 70/30>GL>PVC>MS>SS>Cu, respectively. The 16S rDNA sequence confirmed, heterotrophic biofilm Bacillus sp. (Gram-positive) were dominated in all coupons for all months.
Department of Marine Science, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
Muthukumar
M
Krishnan
Department of Biomedical Science and Environmental Biology, KMU - Kaohsiung Medical University, No. 100, Shin-Chuan 1st Road, Kaohsiung 80708, Taiwan, ROC; Department of Marine Biotechnology and Resources, National Sun Yat-sen University, No. 70, Lienhai Road, Kaohsiung 80424, Taiwan, ROC. Electronic address: hansudahms@yahoo.com.
Hans-Uwe
HU
Dahms
Offshore Platform and Marine Electrochemistry Center (OPMEC), Unit of Central, Electrochemical Research Institute (CECRI), New Harbour Area, Tuticorin 628 004, Tamil Nadu, India.
Palanichamy
P
Seeni
Offshore Platform and Marine Electrochemistry Center (OPMEC), Unit of Central, Electrochemical Research Institute (CECRI), New Harbour Area, Tuticorin 628 004, Tamil Nadu, India.
Subramanian
S
Gopalan
Department of Marine Science, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
Vignesh
V
Sivanandham
Unit of Polar Genomics, Korea Polar Research Institute, 26 Sondomirae-ro, Yeonsu-gu, Incheon, 21990, South Korea.
Kim
K
Jin-Hyoung
Department of Marine Science, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India. Electronic address: james@bdu.ac.in.
Rathinam Arthur
RA
James
D000704:Analysis of Variance; D001419:Bacteria / Q000187:drug effects / Q000302:isolation & purification; D001422:Bacterial Adhesion / Q000187:drug effects; D001483:Base Sequence; D018441:Biofilms / Q000187:drug effects*; D004275:DNA, Ribosomal / Q000235:genetics; D052836:Heterotrophic Processes / Q000187:drug effects; D008670:Metals / Q000494:pharmacology*; D019216:Metals, Heavy / Q000032:analysis; D008856:Microscopy, Fluorescence; D010802:Phylogeny; D012621:Seasons; D012623:Seawater; D013045:Species Specificity
D004275:DNA, Ribosomal; D008670:Metals; D019216:Metals, Heavy
16S rDNA; Bacillus sp.; Biofilm bacteria; Engineering alloys; Heavy metals; Physico-chemical