Application of the MALDI-TOF for identification of Clostridium perfringens strains.
BACKGROUND
The anaerobic bacilli Closfridium perfringens are commonly found in soil and sewage and are also part of the gastrointestinal tract of humans and animals. Food poisoning caused by C. perfringens are regarded the most common diseases transmitted by contaminated food in the world. Bacteria of this species due to their pathogenic properties sustain the constant object of studies in order to elucidate mechanisms of toxinogenesis, to determine the roads of transmission and to develop better diagnostic tools. The objective of the study was to verify, the suitability of the MALDI-TOF method for the identification of C. perfringens species.


METHODS
In order to identify anaerobic bacteria C. perfringens MALDI-TOF method, sequencing of 16S rRNA and detection of cpe and cpa gene by duplex PCR were used.


RESULTS
MALDI-TOF confirmed C. perfringens identification in 39 isolates out of 41 archival isolates under study. The correctness of the results was verified by 16S rRNA sequencing and cpa gene detection.


CONCLUSIONS
The study confirmed the usefulness of MALDI-TOF method in rapid identi- fication of anaerobic bacteria C. perfringens and thereby its applicability epidemiological investigations.
Claudia
C
Brodzik
Ewa
E
Augustynowicz
Agnieszka
A
Korzeniowska-Kowal
Anna
A
Lutyhska
D003016:Clostridium perfringens / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004768:Enterotoxins / Q000235:genetics*; D005517:Foodborne Diseases / Q000382:microbiology; D005798:Genes, Bacterial; D006801:Humans; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017421:Sequence Analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D004768:Enterotoxins; D012336:RNA, Ribosomal, 16S; C035598:enterotoxin, Clostridium