Characterization and complete genome of the virulent Myoviridae phage JD007 active against a variety of Staphylococcus aureus isolates from different hospitals in Shanghai, China.
The implementation of phage therapy is re-emerging with the increase in widespread antibiotic-resistant bacteria.



Staphylococcus phage JD007 was characterized and its complete genome sequence analysed.



Staphylococcus phage JD007 was classified as belonging to the Myoviridae family based on its morphology, as observed by transmission electron microscopy. Its lytic activity was stable between pH 5-11 and below 42 °C; moreover, an absorbance curve showed that nearly 90% of the viral particles had adsorbed to its host after a 20 min co-incubation. The complete genome size is 141,836 bp, making JD007 one of the largest Staphylococcus phages of Myoviridae. No identifiable resistance or virulence genes were found in the JD007 genome. JD007 was able to lyse 95% of S. aureus isolates, including the prevalent ST239-MRSA and ST59-MRSA strains isolated from different hospitals in Shanghai, China, and inhibition assays showed that JD007 could inhibit S. aureus growth at a multiplicity of infection of 0.1.



The results suggested that Staphylococcus phage JD007 can potentially be used in phage therapy or for the detection of S. aureus.
Department of Immunology and Microbiology, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, China. czl_phage@126.com.|Department of Laboratory Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, China. czl_phage@126.com.
Zelin
Z
Cui
Department of Clinical Pharmacy, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, China.
Tingting
T
Feng
Department of Clinical Microbiology, Shanghai Ruijin hospital, Shanghai, 200025, China.
Feifei
F
Gu
Department of Laboratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
Qingtian
Q
Li
Department of Immunology and Microbiology, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, China.
Ke
K
Dong
Department of Immunology and Microbiology, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, China.
Yan
Y
Zhang
Department of Immunology and Microbiology, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, China.
Yongzhang
Y
Zhu
Department of Clinical Microbiology, Shanghai Ruijin hospital, Shanghai, 200025, China.
Lizhong
L
Han
Department of Immunology and Microbiology, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, China.
Jinhong
J
Qin
Department of Immunology and Microbiology, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, China. microbioloy@sjtu.edu.cn.
Xiaokui
X
Guo
D002681:China; D003428:Cross Infection / Q000382:microbiology; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D016679:Genome, Viral; D058507:Host Specificity; D006801:Humans; D006863:Hydrogen-Ion Concentration; D050296:Microbial Viability / Q000187:drug effects / Q000528:radiation effects; D046529:Microscopy, Electron, Transmission; D017900:Myoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology*; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000382:microbiology; D013204:Staphylococcus Phages / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology*; D013211:Staphylococcus aureus / Q000302:isolation & purification / Q000821:virology*; D013696:Temperature; D014771:Virion / Q000648:ultrastructure
D004279:DNA, Viral
Host-range; MRSA; Staphylococcus phage JD007