Discovery of a Highly Virulent Strain ofPhotorhabdus luminescensssp. akhurstiifrom Meghalaya, India.
Photorhabdusis an insect-pathogenic Gram negative enterobacterium found in the gut ofHeterorhabditisnematodes.Photorhabdusis highly virulent to insects, and can kill insects rapidly upon injection at very low concentrations of one to few cells. We characterized the virulence ofPhotorhabdussymbionts isolated from theHeterorhabditisnematodes collected from various parts of India by injecting different concentrations of bacterial cells into fourth instar larval stage of insectGalleria mellonella.Photorhabdus luminescensssp. akhurstiistrain IARI-SGMG3 from Meghalaya was identified as the most virulent of all the tested strains on the basis of LT50and LC50values. This study forms a basis for further investigations on the genetic basis of virulence inPhotorhabdusbacteria.
Division of Nematology, ICAR- Indian Agricultural Research Institute, New Delhi, 110012 India.|Department of Biotechnology and Biosciences, Banasthali Vidyapeeth, Jaipur, Rajasthan India.
Jyoti
J
Kushwah
Division of Nematology, ICAR- Indian Agricultural Research Institute, New Delhi, 110012 India.
Puneet
P
Kumar
Department of Biotechnology and Biosciences, Banasthali Vidyapeeth, Jaipur, Rajasthan India.
Veena
V
Garg
Division of Nematology, ICAR- Indian Agricultural Research Institute, New Delhi, 110012 India.
Vishal Singh
VS
Somvanshi
Galleria; Heterorhabditis; Injectable toxicity; Meghalaya; Photorhabdus; Virulence