Low-melanin containing pullulan production from sugarcane bagasse hydrolysate by Aureobasidium pullulans in fermentations assisted by light-emitting diode.
Pullulan is a polymer produced by Aureobasidium pullulans and the main bottleneck for its industrial production is the presence of melanin pigment. In this study, light-emitting diodes (LEDs) of different wavelengths were used to assist the fermentation process aiming to produce low-melanin containing pullulan by wild strain of A. pullulans LB83 with different carbon sources. Under white light using glucose-based medium, 11.75g.L-1of pullulan with high melanin content (45.70UA540nm.g-1) was obtained, this production improved in process assisted by blue LED light, that resulted in 15.77g.L-1of pullulan with reduced content of melanin (4.46UA540nm.g-1). By using sugarcane bagasse (SCB) hydrolysate as carbon source, similar concentration of pullulan (about 20g.L-1) was achieved using white and blue LED lights, with lower melanin contents in last. Use of LED light was found as a promising approach to assist biotechnological process for low-melanin containing pullulan production.
Department of Biotechnology, Engineering School of Lorena, University of São Paulo, CEP 12602-810, Brazil. Electronic address: teran-2017@usp.br.
Ruly
R
Terán Hilares
Department of Biotechnology, Engineering School of Lorena, University of São Paulo, CEP 12602-810, Brazil.
Camila Ayres
CA
Orsi
Department of Civil and Environmental Engineering, KAIST, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Muhammad Ajaz
MA
Ahmed
Department of Biotechnology, Engineering School of Lorena, University of São Paulo, CEP 12602-810, Brazil.
Paulo Franco
PF
Marcelino
Department of Basic and Environmental Sciences, Engineering School of Lorena, University of São Paulo, CEP 12602-810, Brazil.
Carlos Renato
CR
Menegatti
Department of Biotechnology, Engineering School of Lorena, University of São Paulo, CEP 12602-810, Brazil.
Silvio Silvério
SS
da Silva
Department of Biotechnology, Engineering School of Lorena, University of São Paulo, CEP 12602-810, Brazil.
Júlio César
JC
Dos Santos
D001203:Ascomycota / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects*; D018533:Biomass; D002482:Cellulose / Q000737:chemistry*; D005285:Fermentation / Q000187:drug effects / Q000528:radiation effects; D005936:Glucans / Q000096:biosynthesis*; D005947:Glucose / Q000494:pharmacology; D006868:Hydrolysis; D008027:Light; D008543:Melanins / Q000378:metabolism*; D031786:Saccharum / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
D005936:Glucans; D008543:Melanins; C009109:pullulan; D002482:Cellulose; C027433:bagasse; D005947:Glucose
Enzymatic hydrolysate; Exopolysaccharide (EPS); Light-emitting diode; Melanin pigment; Pullulan; Sugarcane bagasse