The Denitrification Characteristics and Microbial Community in the Cathode of an MFC with Aerobic Denitrification at High Temperatures.
Microbial fuel cells (MFCs) have attracted much attention due to their ability to generate electricity while treating wastewater. The performance of a double-chamber MFC with simultaneous nitrification and denitrification (SND) in the cathode for treating synthetic high concentration ammonia wastewater was investigated at different dissolved oxygen (DO) concentrations and high temperatures. The results showed that electrode denitrification and traditional heterotrophic denitrification co-existed in the cathode chamber. Electrode denitrification by aerobic denitrification bacterium (ADB) is beneficial for achieving a higher voltage of the MFC at high DO concentrations (3.0-4.2 mg/L), while traditional heterotrophic denitrification is conducive to higher total nitrogen (TN) removal at low DO (0.5-1.0 mg/L) concentrations. Under high DO conditions, the nitrous oxide production and TN removal efficiency were higher with a 50 Ω external resistance than with a 100 Ω resistance, which demonstrated that electrode denitrification by ADB occurred in the cathode of the MFC. Sufficient electrons were inferred to be provided by the electrode to allow ADB survival at low carbon:nitrogen ratios (≤0.3). Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) results showed that increasing the DO resulted in a change of the predominant species from thermophilic autotrophic nitrifiers and facultative heterotrophic denitrifiers at low DO concentrations to thermophilic ADB at high DO concentrations. The predominant phylum changed fromFirmicutestoProteobacteria, and the predominant class changed fromBacillitoAlpha, Beta, andGamma Proteobacteria.
School of Environmental Science and Engineering, Chang'an UniversityXi'an, China; Key Laboratory of Subsurface Hydrology and Ecological Effect in Arid Region of Ministry of EducationXi'an, China.
Jianqiang
J
Zhao
School of Environmental Science and Engineering, Chang'an University Xi'an, China.
Jinna
J
Wu
School of Civil Engineering, Chang'an University Xi'an, China.
Xiaoling
X
Li
School of Environmental Science and Engineering, Chang'an University Xi'an, China.
Sha
S
Wang
School of Civil Engineering, Chang'an University Xi'an, China.
Bo
B
Hu
School of Environmental Science and Engineering, Chang'an University Xi'an, China.
Xiaoqian
X
Ding
aerobic denitrifying bacteria; dissolved oxygen; microbial fuel cell; predominant species; simultaneous nitrification and denitrification