Graphene oxide and carboxylated graphene oxide: Viable two-dimensional nanolabels for lateral flow immunoassays.
Graphene oxide (GO) and carboxylated GO were used as labels for lateral flow immunoassays, instead of the conventionally used colloidal gold and colored latex labels. A sensor is demonstrated that enables fast screening for aflatoxin B1(AFB1) as a model analyte using the antibody-GO complex as the recognition element. The visual limit of detection and cut-off value for AFB1are 0.3 and 1ng/mL, respectively. It is shown that GO and carboxylated GO are viable black labels for use in lateral flow assays, one typical advantage being the saving cost (compared to the use of colloidal gold). Qualitative results are achieved within 15min, and the analytical results were in good agreement with the reference LC MS/MS method. The method was successfully applied to the on-site determination of AFB1in agricultural products. In our perception, it opens new possibilities for the screening of other toxins by lateral flow immunoassays using GO and carboxylated GO as labels.
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, PR China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, PR China; Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, PR China. Electronic address: yuli0201010133@hotmail.com.
Li
L
Yu
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, PR China; Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, PR China; Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, 430062, PR China; Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, PR China.
Peiwu
P
Li
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, PR China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, PR China; Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, 430062, PR China.
Xiaoxia
X
Ding
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, PR China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, PR China.
Qi
Q
Zhang
D016604:Aflatoxin B1 / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D002853:Chromatography, Liquid; D018556:Crops, Agricultural / Q000378:metabolism*; D005506:Food Contamination; D006108:Graphite / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D036103:Nanotechnology / Q000379:methods*; D010087:Oxides / Q000032:analysis* / Q000737:chemistry; D053719:Tandem Mass Spectrometry
D010087:Oxides; D006108:Graphite; D016604:Aflatoxin B1
Aflatoxin B(1); Food analysis; Graphene oxide; Immunochromatographic assay; Label; Screening