Proteins in Ionic Liquids: Current Status of Experiments and Simulations.
In the last two decades, while searching for interesting applications of ionic liquids as potent solvents, their solvation properties and their general impact on biomolecules, and in particular on proteins, gained interest. It turned out that ionic liquids are excellent solvents for protein refolding and crystallization. Biomolecules showed increased solubilities and stabilities, both operational and thermal, in ionic liquids, which also seem to prevent self-aggregation during solubilization. Biomolecules can be immobilized, e.g. in highly viscous ionic liquids, for particular biochemical processes and can be designed to some extent by the proper choice of the ionic liquid cations and anions, which can be characterized by the Hofmeister series.
Faculty of Chemistry, Department of Computational Biological Chemistry, University of Vienna, Vienna, Austria. christian.schroeder@univie.ac.at.
Christian
http://orcid.org/0000-0002-2167-5096
C
Schröder
D052578:Ionic Liquids / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D058849:Protein Refolding; D011506:Proteins / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
D052578:Ionic Liquids; D011506:Proteins; D012997:Solvents
Crystallization; Denaturation; Detection; Direct and reversed Hofmeister series; Extraction; Ionic liquid; Separation; Solubility; Stabilization