Polybrominated diphenyl ethers (PBDEs) effects on Chironomus sancticaroli larvae after short-term exposure.
In-vivo effects of polybrominated diphenyl ethers (PBDEs) containing 3, 4 and 5 bromine atoms were tested on fourth-instar larvae of Chironomus sancticaroli (Diptera: Chironomidae) after 48h of exposure, by measuring the activity of the acetyl cholinesterase, alpha and beta esterases and glutathione S-transferase. The PBDE congeners 2,2',4-triBDE (BDE-17), 2,2',4,4'-tetraBDE (BDE-47) and 2,2',4,4',5-pentaBDE (BDE-99) were evaluated at 0.5, 1.0, 2.0 and 3.0ngmL-1. Acetyl cholinesterase activity decreased significantly (p≤0.05) at all evaluated concentrations of the three PBDE congeners, except for larvae exposed to BDE-17 at 1.0 and 2.0ngmL-1. The significant inhibition of acetyl cholinesterase activity ranged from 18% (BDE-47 at 0.5ngmL-1) to 72% (BDE-47 at 2.0ngmL-1). The enzymes alpha and beta esterase were also affected by the three congeners, reducing their activity from 14% (BDE-99 at 1.0ngmL-1) to 52% (BDE-47 at 2.0ngmL-1) and from 7% (BDE-99 at 2.0ngmL-1) to 34% (BDE-47 at 3.0ngmL-1) respectively. Substantial increments in glutathione S-transferase activity were similarly observed, varying from 138% (BDE-99 2.0 at ng mL-1) to 346% (BDE-17 at 1.0ngmL-1). DNA strand breaks were detected exclusively in larvae exposed to BDE-99 at 2.0 and 3.0ngmL-1(H=11.7, p=0.019). These results showed that C. sancticaroli larvae were sensitive to the PBDEs treatments under the experimental conditions.
Zoology Department, Federal University of Paraná, Brazil. Electronic address: anpalacioc@gmail.com.
Angela Maria
AM
Palacio-Cortés
Zoology Department, Federal University of Paraná, Brazil.
Isadora de Lourdes
IL
Signorini-Souza
Chemistry Department, Federal University of Paraná, Brazil.
Emerson Luis
EL
Yoshio Hara
Genetic Department, Federal University of Paraná, Brazil.
Rodrigo Geonildo
RG
Disner
Zoology Department, Federal University of Paraná, Brazil.
Debora
D
Rebechi
Chemistry Department, Federal University of Paraná, Brazil.
Marco Tadeu
MT
Grassi
Genetic Department, Federal University of Paraná, Brazil.
Marta Margarete
MM
Cestari
Zoology Department, Federal University of Paraná, Brazil.
Mario Antônio
MA
Navarro-Silva
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002683:Chironomidae / Q000187:drug effects* / Q000201:enzymology; D004950:Esterases / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism*; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D007814:Larva / Q000187:drug effects* / Q000201:enzymology; D011075:Polybrominated Biphenyls / Q000633:toxicity
D055768:Halogenated Diphenyl Ethers; D011075:Polybrominated Biphenyls; C511295:2,2',4,4'-tetrabromodiphenyl ether; C086401:pentabromodiphenyl ether; D005982:Glutathione Transferase; D004950:Esterases; D000110:Acetylcholinesterase; C533760:tribromodiphenyl ether 28
Acetyl cholinesterase; DNA; Midge