Levofloxacin loaded mesoporous silica microspheres/nano-hydroxyapatite/polyurethane composite scaffold for the treatment of chronic osteomyelitis with bone defects.
Chronic osteomyelitis is a prolonged persistent disease accompanied by bone destruction and sequestrum formation, it is very difficult to treat. Antibiotic loaded polymethyl methacrylate (PMMA) has been used in clinical. However, when PMMA was implanted in the body, the deficiencies is that it is non-biodegradable and a second operation is needed. Here, we synthesize a novel levofloxacin loaded mesoporous silica microspheres/nano-hydroxyapatite/polyurethane composite scaffolds, and evaluated the therapeutic effect in treating chronic osteomyelitis with bone defects in rabbit model compared with bulk PMMA. X-ray, Micro CT, gross pathology as well as immunohistochemical staining were performed at predesignated time points (1, 3, 6 and 12 weeks). Our results demonstrated that the efficiency of mesoporous silica microspheres/nano-hydroxyapatite/polyurethane composite scaffolds loaded with 5 mg levofloxacin was much better at treating bone defects than the other groups. This novel synthetic scaffold may provide a solution for the treatment of chronic osteomyelitis.
Department of Orthopaedic Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
Qi
Q
Wang
Department of Orthopaedic Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
Cheng
C
Chen
Department of Orthopaedic Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
Wen
W
Liu
Department of Orthopaedic Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
Xiaoqiang
X
He
Department of Orthopaedic Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
Nian
N
Zhou
Department of Orthopaedic Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
Dongli
D
Zhang
State Key Laboratory of Oncogenes and Related Genes School of Biomedical Engineering and Med-X Research Institute Shanghai Jiao Tong University, Shanghai 200030, China.
Hongchen
H
Gu
Research Center for Nano-Biomaterials, Analytical &Testing Center, Sichuan University, Chengdu, 610064, China.
Jidong
J
Li
Research Center for Nano-Biomaterials, Analytical &Testing Center, Sichuan University, Chengdu, 610064, China.
Jiaxing
J
Jiang
Department of Orthopaedic Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
Wei
W
Huang
D020341:Absorbable Implants; D000818:Animals; D001861:Bone Regeneration; D001842:Bone and Bones / Q000473:pathology; D002908:Chronic Disease; D004195:Disease Models, Animal; D017886:Durapatite / Q000737:chemistry; D007150:Immunohistochemistry; D064704:Levofloxacin / Q000008:administration & dosage*; D008863:Microspheres; D053758:Nanoparticles / Q000737:chemistry; D010019:Osteomyelitis / Q000000981:diagnostic imaging / Q000188:drug therapy / Q000209:etiology / Q000473:pathology*; D011140:Polyurethanes / Q000737:chemistry; D011817:Rabbits; D011859:Radiography; D012822:Silicon Dioxide / Q000737:chemistry; D054457:Tissue Scaffolds / Q000737:chemistry; D055114:X-Ray Microtomography
D011140:Polyurethanes; D064704:Levofloxacin; D012822:Silicon Dioxide; D017886:Durapatite