Development of a simple and quick immunochromatography method for detection of anti-HPV-16/-18 antibodies.
Immunochromatography (IC) is widely used to detect target molecules in biological fluids. Since this method can be performed without a special technique or device, IC is a convenient way to assess the existence of antibodies or pathogens such as viruses and bacteria, simply and quickly. In this study, we established an IC method to detect serum antibodies against oncogenic human papillomavirus (HPV)-16 and HPV-18 L1 proteins using recombinant L1 proteins produced by silkworms as antigens. Infection of oncogenic HPVs is a major risk factor of cervical cancer, which is one of the most common cancers in women worldwide. We first measured blood sera of two groups by magnetic beads enzyme-linked immunosorbent assay (MB-ELISA). For the first group, sera were collected prospectively from young women who planned to receive HPV vaccination. The second group consisted of children under 20 years of age, non-vaccinated healthy women, vaccinated healthy women, dysplasia, cervical intraepithelial neoplasia III, and cervical cancer patients. We confirmed that standard vaccination doses significantly increased serum HPV antibody concentrations, and the level was sustained at least more than 30 months after vaccination. In contrast, an increase in antibody concentration was not observed in patients with precancerous cervical changes and cervical cancer. We next measured the samples in both groups using the IC method we originally developed, and found that the measurement values of IC highly correlated with those of MB-ELISA. The simple and quick IC method would be a useful tool for rapid monitoring of L1 specific antibody levels in a non-laboratory environment. With less than one drop of serum, our IC can easily detect serum HPV-16/-18 antibodies within 15 minutes, without the need for electronic devices or techniques.
Department of Molecular Medical Research, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kami-Kitazawa, Setagaya-ku, Tokyo, Japan.
Fumiko
F
Endo
Department of Obstetrics and Gynecology, Faculty of Medicine, Mie University, 2-174 Edobashi, Tsu-city, Mie, Japan.
Tsutomu
T
Tabata
Department of Molecular Medical Research, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kami-Kitazawa, Setagaya-ku, Tokyo, Japan.|Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, Japan.
Daichi
D
Sadato
Department of Pediatrics, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome Hospital, 3-18-22 Honkomagome, Bunkyo-ku, Tokyo, Japan.
Machiko
M
Kawamura
Josei-Kokoro-Clinic, 1-1-9 Machiya, Arakawa-ku, Tokyo, Japan.
Noriyuki
N
Ando
Department of Molecular Medical Research, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kami-Kitazawa, Setagaya-ku, Tokyo, Japan.
Keisuke
http://orcid.org/0000-0001-7011-6234
K
Oboki
Department of Molecular Medical Research, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kami-Kitazawa, Setagaya-ku, Tokyo, Japan.
Masako
M
Ukaji
ADTEC Co., LTD. 1693-6 Yokkaichi, Usa-City, Oita, Japan.
Kaoru
K
Kobayashi
ADTEC Co., LTD. 1693-6 Yokkaichi, Usa-City, Oita, Japan.
Yukuharu
Y
Kobayashi
Department of Obstetrics and Gynecology, Faculty of Medicine, Mie University, 2-174 Edobashi, Tsu-city, Mie, Japan.
Tomoaki
T
Ikeda
Department of Molecular Medical Research, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kami-Kitazawa, Setagaya-ku, Tokyo, Japan.
Futoshi
F
Shibasaki
D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D036022:Capsid Proteins / Q000276:immunology; D002846:Chromatography, Affinity / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D052162:Human papillomavirus 16 / Q000276:immunology*; D052161:Human papillomavirus 18 / Q000276:immunology*; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000276:immunology; D030361:Papillomavirus Infections / Q000097:blood / Q000276:immunology* / Q000821:virology; D053918:Papillomavirus Vaccines / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D013997:Time Factors; D014611:Vaccination
D000914:Antibodies, Viral; D036022:Capsid Proteins; C114580:HPV L1 protein, Human papillomavirus; D009856:Oncogene Proteins, Viral; D053918:Papillomavirus Vaccines; D011994:Recombinant Proteins