25-Hydroxycholesterol promotes migration and invasion of lung adenocarcinoma cells.
25-hydroxycholesterol (25-HC) is enzymatically produced by cholesterol 25-hydorxylase in various organs and is involved in many processes, including lipid metabolism, inflammation and the immune response. However, the role of 25-HC in the migration and invasion of lung adenocarcinoma (ADC) cells remains largely unknown. In this study, we demonstrated that 0.1 μM 25-HC promoted ADC cell migration and invasion without affecting cell proliferation, especially after coculture with THP1-derived macrophages. Further investigation showed that 0.1 μM 25-HC significantly stimulated interleukin-1β (IL-1β) secretion in a coculture system and increased the expression of LXR and Snail. IL-1β also mimicked the effect of 25-HC. LXR knockdown notably blocked the 25-HC-induced Snail expression, migration and invasion in both the monoculture system and the coculture system, but it did not impact the effect of IL-1β, which suggested that IL-1β functioned in an LXR-independent manner. These results suggested that 25-HC promoted ADC cell migration and invasion in an LXR-dependent manner in the monoculture system but that in the coculture system, the 25-HC-induced IL-1β secretion enhanced the effect of 25-HC in an LXR-independent manner.
Center Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
Li
L
Chen
Department of Hand and Foot Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
Lishan
L
Zhang
Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
Guozhe
G
Xian
Department of Medical Engineering, Jinan 250021, China.
Yinping
Y
Lv
Center Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China. Electronic address: sphyanlianglin@163.com.
Yanliang
Y
Lin
Department of Burn and Plastic Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China. Electronic address: wyb0616@163.com.
Yibing
Y
Wang
D000072283:A549 Cells; D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology*; D002465:Cell Movement; D006801:Humans; D006888:Hydroxycholesterols / Q000378:metabolism*; D053583:Interleukin-1beta / Q000378:metabolism*; D000071518:Liver X Receptors / Q000378:metabolism*; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology*; D009361:Neoplasm Invasiveness; D010446:Peptide Fragments / Q000378:metabolism*
D006888:Hydroxycholesterols; D053583:Interleukin-1beta; D000071518:Liver X Receptors; D010446:Peptide Fragments; C052994:interleukin-1beta (163-171); C007997:25-hydroxycholesterol
25-Hydroxycholesterol; IL-1β; Invasion; LXR; Lung adenocarcinoma; Migration