Mammalian verprolin CR16 acts as a modulator of ITSN scaffold proteins association with actin.
Actin cytoskeleton rearrangements are required for normal cell functioning, and their deregulation leads to various pathologies. Members of two mammalian protein families - ITSNs (ITSN1 and ITSN2) and verprolins (WIP, CR16 and WIRE) are involved in Cdc42/N-WASP/Arp2/3 signaling pathway-mediated remodeling of the actin cytoskeleton. Recently we demonstrated that ITSNs interact with the actin-regulating protein WIP. Here, we show that other member of verprolin family, CR16, also forms complexes with ITSN1 and ITSN2 in human cell lines. The actin-binding protein CR16 modulates ITSN/β-actin association. Moreover, overexpressed CR16 promoted co-localization of ITSN1 with F-actin in MCF-7 breast cancer cells. Our data demonstrated that CR16 mRNA is expressed in glioblastoma and breast tumors. These findings provide the basis for further functional investigations of the ITSN/CR16 complex that may play an important role in actin remodeling and cellular invasion.
Institute of Molecular Biology and Genetics, 150 Zabolotnogo Street, Kyiv 03143, Ukraine. Electronic address: siryl_2000@yahoo.com.
Sergii
S
Kropyvko
Institute of Molecular Biology and Genetics, 150 Zabolotnogo Street, Kyiv 03143, Ukraine.
Tetyana
T
Gryaznova
Institute of Molecular Biology and Genetics, 150 Zabolotnogo Street, Kyiv 03143, Ukraine.
Dmytro
D
Morderer
Institute of Molecular Biology and Genetics, 150 Zabolotnogo Street, Kyiv 03143, Ukraine.
Alla
A
Rynditch
D008841:Actin Cytoskeleton / Q000378:metabolism*; D000199:Actins / Q000378:metabolism*; D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D006801:Humans; D061986:MCF-7 Cells; D008840:Microfilament Proteins / Q000378:metabolism*; D011485:Protein Binding
D000199:Actins; D033942:Adaptor Proteins, Vesicular Transport; D003598:Cytoskeletal Proteins; C485568:ITSN1 protein, human; C509776:ITSN2 protein, human; D008840:Microfilament Proteins; C562194:WIPF3 protein, human
Actin cytoskeleton; CR16; ITSN; Scaffold protein