Analysis of Cyclin E1 Functions in Porcine Preimplantation Embryonic Development by Fluorescence Microscopy.
Cyclin E1 (CCNE1) is a core component of cell cycle regulation that drives the transition into the S phase. CCNE1 plays critical roles in cell cycle, cell proliferation, and cellular functions. However, the function of CCNE1 in early embryonic development is limited. In the present study, the function and expression of Ccne1 in porcine early parthenotes were examined. Immunostaining experiments showed that CCNE1 localized in the nucleus, starting at the four-cell stage. Knockdown of Ccne1 by double-stranded RNA resulted in the failure of blastocyst formation and induced blastocyst apoptosis. Ccne1 depletion increased expression of the pro-apoptotic gene Bax, and decreased the expression of Oct4 and the rate of inner cell mass (ICM)/trophectoderm formation. The results indicated that CCNE1 affects blastocyst formation by inducing cell apoptosis and ICM formation during porcine embryonic development.
Department of Animal Sciences,Chungbuk National University,Chungbuk,Cheongju 361-763,Republic of Korea.
Jing
J
Guo
Department of Animal Sciences,Chungbuk National University,Chungbuk,Cheongju 361-763,Republic of Korea.
Kyung-Tae
KT
Shin
Department of Animal Sciences,Chungbuk National University,Chungbuk,Cheongju 361-763,Republic of Korea.
Xiang-Shun
XS
Cui
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001755:Blastocyst / Q000187:drug effects; D053624:Blastocyst Inner Cell Mass / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D019927:Cyclin E / Q000235:genetics / Q000494:pharmacology* / Q000502:physiology*; D047108:Embryonic Development / Q000187:drug effects* / Q000502:physiology*; D053595:Embryonic Stem Cells / Q000502:physiology; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D008856:Microscopy, Fluorescence / Q000379:methods*; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D015513:Oncogene Proteins / Q000235:genetics / Q000494:pharmacology / Q000502:physiology; D009865:Oocytes; D012330:RNA, Double-Stranded / Q000032:analysis; D013552:Swine; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D019927:Cyclin E; D050814:Octamer Transcription Factor-3; D015513:Oncogene Proteins; D012330:RNA, Double-Stranded; D051028:bcl-2-Associated X Protein
Cyclin E1; apoptosis; embryonic development; microRNA; pig; pluripotency