Evaluation of the effects of two different bone resorption inhibitors on osteoclast numbers and activity: An animal study.
BACKGROUND
The aim of this study was to evaluate the effects of bone resorption inhibitors, doxycycline (DOX) and erythromycin (EM), on osseous wound healing in rat alveolar socket.


METHODS
In this randomized controlled trial, 45 8-10-week-old male Wistar rats had their maxillary right molar extracted. They were divided into three groups of 15. In Group 1 normal saline, Group 2 DOX, and Group 3 EM were administered at the doses of 5 ml/kg/day, 5 mg/kg/day, and 2 mg/kg/day, respectively, for 7 consecutive days. The rats were sacrificed 7, 14, and 21 days after surgery. Real-time polymerase chain reaction was employed to evaluate the mRNA expression of receptor activator of nuclear factor κB ligand (RANKL) and osteoprotegerin (OPG) and immunohistochemical staining for tartrate-resistant acid phosphatase (TRAP) to determine osteoclasts. The data were analyzed by one-way analysis of variance followed by Tukey'spost hoctest using SPSS version 20. Significant level was set at 0.05.


RESULTS
The results showed that when drug-treated groups compared to control groups, RANKL gene expression significantly decreased, TRAP+cells decreased on day 7. The RANKL/OPG ratios in the first two weeks in the test groups were significantly lower than the control group. There was no significant difference in the studied indices between DOX and EM groups.


CONCLUSIONS
Following administration of DOX and EM, the number of osteoclasts and RANKL/OPG ratio decreased suggesting their anti-osteoclastogenesis activity. These two drugs have no advantage over each other in increasing the bone formation.
Department of Periodontology, Dental Implants Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Narges
N
Naghsh
Department of Oral and Maxillofacial Pathology, Dental Implants Research Center, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
Sayed Mohammad
SM
Razavi
Department of Pharmacology, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran.
Mohsen
M
Minaiyan
Department of Periodontology, Dental Implants Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Mohammad
M
Shahabooei
Department of Periodontology, Dental Research Center, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
Reza
R
Birang
Department of Periodontology, Dental Implants Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Parichehr
P
Behfarnia
Department of Oral and Maxillofacial Medicine, School of Dentistry, Qom University of Medical Sciences, Qom, Iran.
Samira
S
Hajisadeghi
Immunohistochemistry; real-time polymerase chain reaction; tartrate-resistant acid phosphatase