Simplified detection of the hybridized DNA using a graphene field effect transistor.
Detection of disease-related gene expression by DNA hybridization is a useful diagnostic method. In this study a monolayer graphene field effect transistor (GFET) was fabricated for the detection of a particular single-stranded DNA (target DNA). The probe DNA, which is a single-stranded DNA with a complementary nucleotide sequence, was directly immobilized onto the graphene surface without any linker. The VDiracwas shifted to the negative direction in the probe DNA immobilization. A further shift of VDiracin the negative direction was observed when the target DNA was applied to GFET, but no shift was observed upon the application of non-complementary mismatched DNA. Direct immobilization of double-stranded DNA onto the graphene surface also shifted the VDiracin the negative direction to the same extent as that of the shift induced by the immobilization of probe DNA and following target DNA application. These results suggest that the further shift of VDiracafter application of the target DNA to the GFET was caused by the hybridization between the probe DNA and target DNA.
Centre for Nanoscience and Technology, Anna University, Chennai, India; Nanotechnology Innovation Station, National Institute for Materials Science, Tsukuba, Japan.
Arun Kumar
AK
Manoharan
Nanotechnology Innovation Station, National Institute for Materials Science , Tsukuba , Japan.
Shanmugavel
S
Chinnathambi
Centre for Nanoscience and Technology, Anna University , Chennai , India.
Ramasamy
R
Jayavel
Nanotechnology Innovation Station, National Institute for Materials Science, Tsukuba, Japan; Graduate School of Life Science, Hokkaido University, Sapporo, Japan.
Nobutaka
N
Hanagata
208 Sensors and actuators; 40 Optical, magnetic and electronic device materials; DNA; Dirac point; Graphene; field effect transistor