Immunomodulatory effects of Bifidobacterium bifidum 231 on trinitrobenzenesulfonic acid-induced ulcerative colitis in rats.
OBJECTIVE
Ulcerative colitis and Crohn's disease are two important chronic Inflammatory bowel diseases (IBD) characterized by prominent intestinal inflammation. Probiotics are the bacteria that promote the host health by its immunomodulatory activity. The present study investigated the correlation between in vitro adhesion and immunomodulatory properties, and to assess the therapeutic potential of Bifidobacterium bifidum 231 (BIF 231), a new strain of probiotic in ulcerative colitis in rats.


METHODS
In vitro adhesion assays and immunomodulatory effect of BIF 231 on interleukins (IL-1β and IL-10) in IEC-6 cell lines were quantified by gram staining, scanning electron microscopy and q-PCR respectively. Colitis was induced by intra-rectal instillation of trinitrobenzenesulfonic acid. Colitis was evaluated by alterations in colon gross morphology, histologically and biochemically. Colonic interleukin-1β (IL-1β) and interleukin-10 (IL-10) mRNA and protein expression were assessed by q-PCR, ELISA and western blot.


RESULTS
BIF 231 showed better adhesion and immunomodulation by up-regulating IL-10 levels in IEC-6 cell lines. In vivo studies with treatment of BIF 231 (1.4×1011CFU/rat/day) revealed anti-inflammatory effects both macroscopically and histologically. BIF 231 lowered TBARS, nitric oxide and augmented GSH levels. BIF 231 treatment to colitic rats down regulated IL-1β levels with concurrent increase in IL-10 levels.


CONCLUSIONS
BIF 231 exerted beneficial in vitro adhesion and immunomodulatory properties which facilitated the recovery of the damaged tissue in TNBS-induced colitis.
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad 500 030, India. Electronic address: satish513512@gmail.com.
C S V
CS
Satish Kumar
Department of Livestock Products Technology, College of Veterinary Science, Rajendranagar, Hyderabad 500 030, India. Electronic address: kkkreddy5@rediffmail.com.
K
K
Kondal Reddy
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad 500 030, India. Electronic address: bhupalvets@gmail.com.
G
G
Boobalan
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad 500 030, India. Electronic address: gopalareddy123@rediffmail.com.
A
A
Gopala Reddy
Department of Veterinary Pathology, NTR College of Veterinary Science, Gannavaram 521102, India. Electronic address: drsudha84@gmail.com.
C H
CH
Sudha Rani Chowdhary
Department of Molecular Genetics and Breeding, Directorate of Poultry Research, Rajendranagar, Hyderabad 500 030, India. Electronic address: biotechvinoth@gmail.com.
A
A
Vinoth
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad 500 030, India. Electronic address: dr.koppakajayakanth@gmail.com.
K
K
Jayakanth
Department of Veterinary Pharmacology & Toxicology, NTR College of Veterinary Science, Gannavaram 521102, India. Electronic address: raogs64@rediffmail.com.
G
G
Srinivasa Rao
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D001422:Bacterial Adhesion; D000069985:Bifidobacterium bifidum / Q000737:chemistry*; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000276:immunology* / Q000382:microbiology / Q000628:therapy*; D003907:Dexamethasone / Q000627:therapeutic use; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D056747:Immunomodulation; D019936:Probiotics / Q000737:chemistry / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D014302:Trinitrobenzenesulfonic Acid / Q000737:chemistry
D000893:Anti-Inflammatory Agents; D003907:Dexamethasone; D014302:Trinitrobenzenesulfonic Acid
BIF 231; Colitis; Cytokines; Free radicals; IEC-6; NO