One-step micromolding of complex 3D microchambers for single-cell analysis.
Herein we examined the extent of replicability of the PDMS microchamber device transferred from the master mold with complex 3D structures fabricated via micro stereolithography. Due to the elastomeric properties of PDMS, the reversely tapered micromold, with the diameter ratio of ∼5 from the largest to the narrowest part, was precisely transferred without breaking. We obtained the mathematical model to estimate the stress exerted on the mold during the demolding process. Finally, we tested the applicability of this unusual microchamber for single-cell trapping and an enzyme assay.
Department of Precision Mechanics, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo, 112-8551, Japan. suzuki@mech.chuo-u.ac.jp.
Hiroaki
0000-0002-8899-0955
H
Suzuki
Department of Precision Mechanics, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo, 112-8551, Japan. suzuki@mech.chuo-u.ac.jp.
Kenta
K
Mitsuno
Laboratory for Integrative Omics, RIKEN Quantitative Biology Center (QBiC), 6-2-3 Furuedai, Suita, Osaka, 565-0874, Japan and Laboratory for Immunogenetics, RIKEN Center for Integrative Medical Sciences (IMS), 1-7-22, Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan and JST PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012, Japan.
Katsuyuki
K
Shiroguchi
Department of Precision Mechanics, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo, 112-8551, Japan. suzuki@mech.chuo-u.ac.jp and JSPS, 5-3-1, Kojimachi, Chiyoda-ku, Tokyo, 102-0083, Japan.
Mamiko
M
Tsugane
Department of Precision Mechanics, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo, 112-8551, Japan. suzuki@mech.chuo-u.ac.jp.
Taiji
T
Okano
Department of Precision Mechanics, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo, 112-8551, Japan. suzuki@mech.chuo-u.ac.jp.
Tetsuji
T
Dohi
Department of Precision Mechanics, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo, 112-8551, Japan. suzuki@mech.chuo-u.ac.jp.
Tomoaki
T
Tsuji
D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D004129:Dimethylpolysiloxanes; D004867:Equipment Design; D006801:Humans; D019169:Jurkat Cells; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D059010:Single-Cell Analysis / Q000295:instrumentation* / Q000379:methods
D004129:Dimethylpolysiloxanes