Citotoxicity evaluation of three dental adhesives on vero cellsin vitro.
BACKGROUND
To evaluate,in vitro, the potential cytotoxicity of three different dental adhesives systems (Adper Single Bond 2 -SB, Silorane System Adhesive Bond -SSAB and Single Bond Universal -SBU) on cultivated Vero cells after different contact times.


METHODS
The cells were cultured in a concentration of 2 x 105 cells/mL for 24h and grown to sub-confluent monolayers. VERO cells were exposed to 25µl of conditioned extracts obtained from 24h, 48h and 72h immersion of adhesive samples in culture medium (DMEM), immediately after polymerization. Fresh DMEM was used as negative control. Cell metabolism was evaluated by the MTT assay (3-(4,5-dimethylthiazol-2-yl)-2, 5diphenyl-tetrazolium bromide). The data were analyzed statistically by ANOVA, considering a significance of 5%.


RESULTS
The values of cell viability ranged from 94.2% at 72h (SBU) to 109.6% at 48h (SB). The mean percentage of viability after exposure to the extracts of SB, SSAB and SBU were 103.2%, 100.63% and 97.43%, respectively. There was no statistically significant difference (p= 0.342) between the experimental and negative control groups.


CONCLUSIONS
At all exposure times, all adhesives tested in this study presented no cytotoxicity to Vero cellsin vitro.Key words:Biocompatibility, cytotoxicity, dental adhesives, Vero cells.
MSc student, Department of Clinical and Preventive Dentistry, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
Raisa-Queiroz
RQ
Catunda
Professor, Department of Histology and Embryology, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
Jeymesson-Raphael-Cardoso
JR
Vieira
Professor, Department of Histology and Embryology, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
Erwelly-Barros
EB
de Oliveira
Undergraduate, Department of Histology and Embryology, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
Eliete-Cavalcanti
EC
da Silva
PhD student, Department of Clinical and Preventive Dentistry, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
Veruska-Lima-Moura
VL
Brasil
Professor, Department of Clinical and Preventive Dentistry, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
Danyel-Elias-da Cruz
DC
Perez