Fe3+/SeO42-dual doped nano hydroxyapatite: A novel material for biomedical applications.
Dual ions substituted hydroxyapatite (HA) received attention from scientists and researchers in the biomedical field owing to their excellent biological properties. This paper presents a novel biomaterial, which holds potential for bone tissue applications. Herein, we have successfully incorporated ferric (Fe3+)/selenate ( SeO42-) ions into the HA structure (Ca10-x-yFey(PO4)6-x(SeO4)x(OH)2-x-yOy) (Fe-SeHA) through a microwave refluxing process. The Fe-SeHA materials were characterized by X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and field emission scanning electron microscopy (FESEM). XRD and FTIR analyses revealed that Fe-SeHA samples were phase pure at 900°C. FESEM images showed that formation of rod-like shaped particles was inhibited dramatically with increasing Fe3+amount. The Vickers hardness (HV) test showed that hardness values increased with increasing Fe3+concentrations. Optical spectra of Fe-SeHA materials contained broadband over (200-600) nm. In vitro degradation and bioactivity tests were conducted in simulated body fluid (SBF). The incorporation of Fe3+/ SeO42- ions into the HA structure resulted in a remarkably higher degradation rate along with intense growth of apatite granules on the surface of the Fe-SeHA discs with Ca/P ratio of 1.35-1.47. In vitro protein adsorption assay was conducted in fetal bovine serum (FBS) and it was observed that the adsorption of serum proteins on Fe-SeHA samples significantly increased with increasing Fe3+concentration. In vitro cytotoxicity tests were performed with human fetal osteoblast (hFOB) cell line and the results demonstrated that hFOB cells attached and proliferated faster on the Fe-SeHA materials compared to pure HA showing that Fe-SeHA materials were cytocompatible. ALP activity and intracellular calcium of hFOB cells on 1Fe-SeHA discs were statistically higher than pure HA, suggesting that presence of Fe3+ion supported osteogenic differentiation of hFOB cells. Our results suggest that 1Fe-SeHA (0.2M Fe3+/0.5M SeO42- co-doped HA) material could be considered as a promising candidate material for orthopedic applications. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 340-352, 2018.
Department of Biomedical Engineering, Faculty of Engineering, Karabuk University, 78050, Karabuk, Turkey.
Ammar Z
AZ
Alshemary
Department of Engineering Sciences, Middle East Technical University, Ankara, 06800, Turkey.
Ahmet
A
Engin Pazarceviren
Department of Engineering Sciences, Middle East Technical University, Ankara, 06800, Turkey.
Aysen
A
Tezcaner
Department of Engineering Sciences, Middle East Technical University, Ankara, 06800, Turkey.
Zafer
Z
Evis
D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology; D005290:Ferric Compounds / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008422:Materials Testing; D049329:Nanostructures / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D064586:Selenic Acid / Q000737:chemistry / Q000494:pharmacology
D005290:Ferric Compounds; D017886:Durapatite; D064586:Selenic Acid
Fe3+/SeO42−ions; dual doped HA; hFOB cells; in vitro degradation; novel biomaterial