The study of polyphenolic compounds profile of some Rosmarinus officinalis L. extracts.
The purpose of this study was to compare the polyphenol profile of different Rosemary (Rosmarinus officinalis) extracts obtained from dry and fresh herb and the evaluation of their antioxidant effect. There were studied the polyphenols from hydroalcoholic extracts (1:5- dry plant:solvent) obtained from fresh respectively dry plant and the gemmotherapic extract obtained from fresh plant (1:20- dry plant: solvent). The polyphenol profile was evaluated by UV-Vis spectrophotometry, TLC and HPLC. The total polyphenol respectively flavonoids content were determined by UV-V is spectrophotometry. The antioxidant effect was evaluated by DPPH, ABTS, FRAP, CUPRAC and silver nanoparticle (SNP) methods. The hydroalcoholic extract obtained from fresh plant contains the highest concentration of total polyphenols, (0.601mg/ml rosmarinic acid), total flavonoids, (0.270mg/ml luteoline) and rosmarinic acid (0.350 mg/ml). The less concentrated is the gemmotherapic extract, but also the extraction ratio is higher than the hydroalcoholic extracts. The high content in polyphenols of the mentioned hydroalcoholic extract was confirmed by highest values of antioxidant activity: 39.1ml (DPPH), 7.7 ml (ABTS), 698mM ET/100 ml (FRAP), 1947 mM ET/100 ml (CUPRAC), 4570mM ET/100 ml (SNP). These differences in the polyphenols profiles show the importance of use the fresh plants for obtaining the good quality extracts.
Vasile Goldi Western University of Arad, Faculty of Pharmacy, Rebreanu Street, Arad, Romania / SC PlantExtrakt SRL, 407059, Rădaia, Cluj, Romania.
Neli-Kinga
NK
Olah
Vasile Goldi Western University of Arad, Faculty of Pharmacy, Rebreanu Street, Arad, Romania.
Gyongyi
G
Osser
SC PlantExtrakt SRL, 407059, Rădaia, Cluj, Romania.
Ramona Flavia
RF
Câmpean
SC PlantExtrakt SRL, 407059, Rădaia, Cluj, Romania.
Flavia Roxana
FR
Furtuna
Iuliu Haieganu University of Medicine and Pharmacy, Cluj-Napoca, Faculty of Pharmacy, Creangă Street, Cluj-Napoca, Romania.
Daniela
D
Benedec
Iuliu Haieganu University of Medicine and Pharmacy, Cluj-Napoca, Faculty of Pharmacy, Creangă Street, Cluj-Napoca, Romania.
Lorena
L
Filip
National Institute for Research and Development of Isotopic and Molecular Technologies, 1 Donath str, Cluj-Napoca, Romania.
Oana
O
Raita
Daniela
D
Hanganu
D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D010084:Oxidation-Reduction; D000072257:Oxygen Radical Absorbance Capacity; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D018520:Plant Shoots / Q000737:chemistry; D010946:Plants, Medicinal; D059808:Polyphenols / Q000032:analysis* / Q000494:pharmacology; D027542:Rosmarinus / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013451:Sulfonic Acids / Q000737:chemistry
D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; D012997:Solvents; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl