Neuroprotective effects of extracellular DJ-1 on reperfusion injury in SH-SY5Y cells.
Using an in vitro model of ischemic stroke we treated differentiated SH-SY5Y cells to oxygen-glucose deprivation (OGD) followed by a reperfusion period where normal growth conditions were restored. Cells undergoing OGD exhibited significant cell death as measure by propidium iodide staining. However, cells treated with exogenous extracellular DJ-1 during reperfusion exhibited significant rescue from OGD-induced cell death.
Faculty of Health Sciences, Simon Fraser University, Burnaby, BC, V5A 1S6, Canada.
Jay
J
Han
Faculty of Health Sciences, Simon Fraser University, Burnaby, BC, V5A 1S6, Canada.
Beryl
B
Luk
Faculty of Health Sciences, Simon Fraser University, Burnaby, BC, V5A 1S6, Canada.
Frank J S
0000-0002-5768-3776
FJ
Lee
D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D005947:Glucose / Q000172:deficiency*; D006801:Humans; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism*; D000071617:Protein Deglycase DJ-1 / Q000494:pharmacology*
D018696:Neuroprotective Agents; D000071617:Protein Deglycase DJ-1; D005947:Glucose; D010100:Oxygen
OGD; PARK7; cell death; hypoxia