An ultrasensitive fluorescent probe for rapid determination of thiophenols.
An ultrasensitive fluorescent probe TPP was developed for the highly selective detection of thiophenols based on excited-state intramolecular proton transfer (ESIPT) mechanism, its synthesis through a simple straightforward combination of HBT fluorophore with 2,4-dinitrobenzene functional group. A kinetic study of TPP towards thiophenol displayed a fast response time (~150s) and significant turn-on fluorescence enhancement (~60 fold). Selective and competitive experiments exhibited an excellent selectivity of TPP toward thiophenols over biothiols (Cys, GSH) and other aliphatic thiols or nucleophiles. Using this ultrasensitive probe (LOD, 1.05×10-8M), we have successfully monitored and quantified highly toxic thiophenols in aqueous media and real-water samples.
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, No.163 Xianlin Road, Nanjing 210023, China; Green Chemical Engineering Technology Research Center, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China.
Lin
L
Lin
Green Chemical Engineering Technology Research Center, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China.
Xiangqiong
X
Zeng
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, No.163 Xianlin Road, Nanjing 210023, China.
Yuning
Y
Shen
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, No.163 Xianlin Road, Nanjing 210023, China. Electronic address: zhuhl@nju.edu.cn.
Hailiang
H
Zhu
College of Chemistry and Materials Science, Nanjing Normal University, No.1 Wunyuan Road, Nanjing 210023, China; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, No.163 Xianlin Road, Nanjing 210023, China. Electronic address: yongqian@njnu.edu.cn.
Yong
Y
Qian
ESIPT; Fluorescent probe; Thiophenols; Turn-on