Artificial Antigen-Presenting Cells for Immunotherapies.
Artificial antigen-presenting cells (aAPCs) overcome many of the limitations of biologically based adoptive immunotherapy protocols. While these acellular systems can be designed with a variety of parameters, including material type, diameter, and proliferative signals for T cells, we outline methods to formulate and characterize a comprehensive polymeric microparticle aAPC platform. These aAPCs, which can be reproducibly fabricated in large quantities, efficiently stimulate antigen-specific T cell activation and proliferation by both paracrine cytokine signals and engagement of T cell surface proteins.
Department of Biomedical Engineering, Yale University, 55 Prospect St., New Haven, CT, 06520, USA.
Alyssa L
AL
Siefert
Department of Biomedical Engineering, Yale University, 55 Prospect St., New Haven, CT, 06520, USA.
Tarek M
TM
Fahmy
Department of Biomedical Engineering, Yale University, 55 Prospect St., New Haven, CT, 06520, USA. Dongin.Kim@pharmacy.tamhsc.edu.|Department of Pharmaceutical Sciences, Irma Lerma Rangel College of Pharmacy, Texas A&M HSC, Reynolds Medical Building Suite 159, Mail Stop 1114, College Station, TX, 77843-1114, USA. Dongin.Kim@pharmacy.tamhsc.edu.
Dongin
D
Kim
D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000276:immunology; D058045:Artificial Cells / Q000276:immunology*; D001360:Avidin; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D006649:Histocompatibility Antigens / Q000737:chemistry / Q000276:immunology; D006801:Humans; D007167:Immunotherapy; D016219:Immunotherapy, Adoptive; D019344:Lactic Acid / Q000737:chemistry; D008863:Microspheres; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013499:Surface Properties; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism
D000906:Antibodies; D006649:Histocompatibility Antigens; D001360:Avidin; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
Immunotherapy; Microparticle; PLGA; Paracrine delivery; Polymer; aAPC