Characterization of a small GTP-binding protein gene TaRab18 from wheat involved in the stripe rust resistance.
The stripe rust resistance gene, Yr26, is commonly used in wheat production. Identification of Yr26 resistance related genes is important for better understanding of the resistance mechanism. TaRab18, a putative small GTP-binding protein, was screened as a resistance regulated gene as it showed differential expression between the Yr26-containing resistant wheat and the susceptible wheat at different time points after Pst inoculation. TaRab18 contains four typical domains (GI to GIV) of the small GTP-binding proteins superfamily and five domains (RabF1 to RabF5) specific to the Rab subfamily. From the phylogenetic tree that TaRab18 was identified as belonging to the RABC1 subfamily. Chromosome location analysis indicated that TaRab18 and its homeoalles were on the homeologous group 7 chromosomes, and the Pst induced TaRab18 was on the 7 B chromosome. Functional analysis by virus induced gene silencing (VIGS) indicated that TaRab18 was positively involved in the stripe rust resistance through regulating the hypersensitive response, and Pst can develop on the leaves of TaRab18 silenced 92R137. However, over-expression of TaRab18 in susceptible Yangmai158 did not enhance its resistance dramatically, only from 9 grade in Yangmai158 to 8 grade in the transgenic plant. However, histological observation indicated that the transgenic plants with over-expressed TaRab18 showed a strong hypersensitive response at the early infection stage. The research herein, will improve our understanding of the roles of Rab in wheat resistance.
Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing 210095, China; Key Laboratory of Wheat Biology and Genetic Improvement on Low and Middle Yangtze River Valley Wheat Region (Ministry of Agriculture), Institute of Agricultural Science of the Lixiahe District in Jiangsu Province, Yangzhou 225007, China. Electronic address: znjiang79@aliyun.com.
Zhengning
Z
Jiang
Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing 210095, China. Electronic address: 2014101065@njau.edu.cn.
Hui
H
Wang
Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing 210095, China. Electronic address: 2011101082@njau.edu.cn.
Guoqin
G
Zhang
Key Laboratory of Wheat Biology and Genetic Improvement on Low and Middle Yangtze River Valley Wheat Region (Ministry of Agriculture), Institute of Agricultural Science of the Lixiahe District in Jiangsu Province, Yangzhou 225007, China. Electronic address: hrzhao@136.com.
Renhui
R
Zhao
Key Laboratory of Wheat Biology and Genetic Improvement on Low and Middle Yangtze River Valley Wheat Region (Ministry of Agriculture), Institute of Agricultural Science of the Lixiahe District in Jiangsu Province, Yangzhou 225007, China. Electronic address: btd@wheat.org.cn.
Tongde
T
Bie
Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing 210095, China. Electronic address: rqz@njau.edu.cn.
Ruiqi
R
Zhang
Key Laboratory of Wheat Biology and Genetic Improvement on Low and Middle Yangtze River Valley Wheat Region (Ministry of Agriculture), Institute of Agricultural Science of the Lixiahe District in Jiangsu Province, Yangzhou 225007, China. Electronic address: gdr@wheat.org.cn.
Derong
D
Gao
Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing 210095, China. Electronic address: xingliping@njau.edu.cn.
Liping
L
Xing
Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing 210095, China. Electronic address: caoaz@njau.edu.cn.
Aizhong
A
Cao
D001483:Base Sequence; D001487:Basidiomycota / Q000235:genetics; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D060467:Disease Resistance; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D054884:Host-Pathogen Interactions; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D030821:Plants, Genetically Modified; D014170:Transformation, Genetic; D014908:Triticum / Q000166:cytology / Q000235:genetics* / Q000382:microbiology; D014775:Virus Activation; D020691:rab GTP-Binding Proteins / Q000235:genetics*
D010940:Plant Proteins; D020691:rab GTP-Binding Proteins
Rab; Stripe rust; Transgenic plants; Virus induced gene silencing (VIGS); Wheat; Yr26