Evaluation of Cell Counting in Body Fluids: Comparison of Two Automated Hematology Analyzers with Manual Microscopy.
BACKGROUND
Manual microscopic analysis (MMA) of body fluids has been widely replaced by automated systems. The aim of this study was to assess the performances of the Sysmex XN-1000 (XN-1000) and UniCel DxH800 (DxH800) for body fluid analysis and compare their results with MMA and with each other.


METHODS
Red blood cell (RBC), WBC and WBC-differential counts of 142 body fluid samples (7 cerebrospinal, 28 pleural, 107 ascitic fluids) were performed using DxH800, XN-1000, and MMA.


RESULTS
The within-run and between-days CVs% were lower than 10% for both systems except MONO of DxH800. Both analyzers demonstrated good linearity and minimal carry-over. The comparison of the XN-1000 and DxH800 with manual counting and each other revealed good correlation (r > 0.90 for both).


CONCLUSIONS
Automated systems introduce standardized and accurate performances to analyze biologic fluids. They are also beneficial for reducing turn-around time and laboratory costs.
Sema
S
Genc
Elmire
E
Dervisoglu
Dilara
D
Omer
Emine
E
Kucukates
Beyhan
B
Omer
Evin
E
Ademoglu
D057205:Automation, Laboratory; D001826:Body Fluids / Q000166:cytology*; D004867:Equipment Design; D004906:Erythrocyte Count / Q000295:instrumentation*; D006405:Hematology / Q000295:instrumentation*; D006801:Humans; D007958:Leukocyte Count / Q000295:instrumentation*; D008853:Microscopy; D011237:Predictive Value of Tests; D015203:Reproducibility of Results