Gene Specific Impedimetric Bacterial DNA Sensor for Rheumatic Heart Disease.
An impedimetricmgagene specific DNA sensor was developed by immobilization of single stranded DNA probe onto the screen printed modified gold-dendrimer nanohybrid composite electrode for early and rapid detection ofS. pyogenesin human throat swab samples causing rheumatic heart disease. Electrochemical impedance response was measured after hybridization with bacterial single stranded genomic DNA (ssG-DNA) with probe. The sensor was found highly specific toS. pyogenesand can detect as low as 0.01 ng ssDNA in 6 µL sample only in 30 min. The nanohybrid sensor was also tested with non-specific pathogens and characterized by FTIR. An early detection of the pathogenS. pyogenesin human can save damage of mitral and aortic heart valves (rheumatic heart disease) by proper medical care.
CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi, 110007 India.|Academy of Scientific and Innovative Research (AcSIR), New Delhi, 110025 India.
Swati
S
Singh
CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi, 110007 India.
Ankur
A
Kaushal
National Centre for Disease Control, Sham Nath Marg, Delhi, 110054 India.
Sunil
S
Gupta
CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi, 110007 India.|Academy of Scientific and Innovative Research (AcSIR), New Delhi, 110025 India.
Ashok
A
Kumar
DNA sensor; Impedimetric; Nanohybrid; PAMAM; Rheumatic heart disease; S. pyogenes