Saccharopolyspora Species: Laboratory Maintenance and Enhanced Production of Secondary Metabolites.
Saccharopolyspora spp. are aerobic, Gram-positive, non-acid-fast, and non-motile actinomycetes. Various species of the genus Saccharopolyspora have been reported with an ability to produce various bioactive compounds for pharmaceutical and agricultural uses. This unit includes general protocols for the laboratory maintenance of Saccharopolyspora species, including growth in liquid medium, growth on solid agar, long-term storage, and generation of a higher producer strain by mutagenesis. Saccharopolyspora spinosa ATCC 49460 is used as a prototype for explaining the considerations for efficient laboratory maintenance of Saccharopolyspora spp. Saccharopolyspora spinosa is a producer of spinosad, a prominent insecticide with selective activity against various insects. © 2017 by John Wiley & Sons, Inc.
Department of Life Science and Biochemical Engineering, Sun Moon University, Chungnam, Republic of Korea.
Dipesh
D
Dhakal
Department of Life Science and Biochemical Engineering, Sun Moon University, Chungnam, Republic of Korea.
Anaya Raj
AR
Pokhrel
Department of Life Science and Biochemical Engineering, Sun Moon University, Chungnam, Republic of Korea.
Amit Kumar
AK
Jha
Center for Molecular Biology, Institute of Research and Development, Duy Tan University, Danang, Vietnam.
Nguyen Huy
NH
Thuan
Department of Life Science and Biochemical Engineering, Sun Moon University, Chungnam, Republic of Korea.|Department of BT-Convergent Pharmaceutical Engineering, Sun Moon University, Chungnam, Republic of Korea.
Jae Kyung
JK
Sohng
D001431:Bacteriological Techniques / Q000379:methods*; D001688:Biological Products / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D060847:Metabolic Engineering / Q000379:methods*; D016296:Mutagenesis; D011309:Preservation, Biological / Q000379:methods; D016930:Saccharopolyspora / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*
D001688:Biological Products; D003470:Culture Media
Saccharopolyspora; UV mutagenesis; medium; spinosad; storage