Effect of structure on sensing performance of a target induced signaling probe shifting DNA-based (TISPS-DNA) sensor.
A type of "signal on" displacement-based sensors named target induced signaling probe shifting DNA-based (TISPS-DNA) sensor were developed for a designated DNA detection. The signaling mechanism of the signaling probe (SP) shifting different from the classical conformation/flexibility change mode endows the sensor with high sensitivity. Through using thiolated or no thiolated capturing probe (CP), two 3-probe sensing structures, sensor-1 and sensor-2, were designed and constructed. The systematical comparing research results show that both sensors exhibit some similarities or big differences in sensing performance. On the one hand, the similarity in structures determines the similarity in some aspects of signaling mechanism, background signal, signal changing form, anti-fouling ability and versatility; on the other hand, the slight difference in structures also results in two opposite hybridization modes of gradual increasing resistance and gradual decreasing resistance which can affect the hybridization efficiency between the assistant probe (AP) and the SP, further producing some big differences in sensing performance, for example, apparently different signal enhancement (SE) change, point mutation discrimination ability and response speed. Under the optimized fabrication and detection conditions, both sensors feature high sensitivity for target DNAs with the detection limits of ∼10 fM for sensor-1 and ∼7 fM for sensor-2, respectively. Among many acquired sensing virtues, the sensor-1 shows a peculiar specificity adjustability which is also a highlight in this work.
College of Chemical Engineering, Guangdong University of Petrochemical Technology, Maoming 525000, China.
Xiang
X
Yu
Department of Chemistry and Chemical Engineering, Yangtze Normal University, Fuling, Chongqing 408100, China; College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150040, China.
Zhigang
Z
Yu
College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150040, China.
Fengqin
F
Li
College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150040, China.
Yanmei
Y
Xu
Department of Electronic Science and Technology, Harbin University of Science and Technology, Harbin 150080, China.
Xunjun
X
He
Key Laboratory on Luminescence and Real-time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
Lan
L
Xu
Department of Chemistry and Chemical Engineering, Yangtze Normal University, Fuling, Chongqing 408100, China.
Wenbing
W
Shi
College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150040, China.
Guiling
G
Zhang
College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150040, China.
Hong
H
Yan
D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000235:genetics; D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics; D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization / Q000379:methods*; D017354:Point Mutation
D015342:DNA Probes; D004247:DNA
DNA detection; Displacement-based; Electrochemical sensor; Point mutation discrimination; Signal on