CD40 polymorphism in cervical carcinoma in a subset of Malaysian population.
OBJECTIVE
The aim of this study was to determine the allelic frequency of single nucleotide polymorphisms (SNPs) in the human CD40 gene in cervical cancer.


METHODS
A total of 200 cases were selected from the records of the Department of Pathology, Hospital Tuanku Jaafar, Seremban, Malaysia. The samples were collected in three separate groups: cervicitis (n = 61), cervical intraepithelial neoplasia (n = 69), and cervical carcinoma (n = 70). The patients' demographic data and the respective paraffin-embedded tissue samples from Hospital Tuanku Jaafar, Seremban were obtained upon consent. The sample tissues were submitted for DNA extraction using G-spin Total DNA Extraction Kit. DNA obtained was then submitted for nested PCR before restriction enzyme digestion.


RESULTS
SNP rs1883832 showed higher prevalence of T alleles in the cervical carcinoma group compared to the control groups and in rs3765459, a higher prevalence of G alleles in the cervical carcinoma group was noted. The results of rs1800686 and rs4810485 were insignificant.


CONCLUSIONS
The data from our study indicates a potential association between the rs1883832 and rs3765459 CD40 gene polymorphism and susceptibility to cervical cancer.
Department of Pathology, International Medical University, Kuala Lumpur, Malaysia.
Purushotham
P
Krishnappa
Department of Pathology, International Medical University, Kuala Lumpur, Malaysia.
Hong Mun
HM
Kong
Department of Pathology, Hospital Tuanku Ja'afar, Seremban, Malaysia.
Ibtisam Binti
IB
Mohamad
Department of Pathology, International Medical University, Kuala Lumpur, Malaysia.
Kenny
K
Voon
Department of Pathology, International Medical University, Kuala Lumpur, Malaysia.
Sushela Devi
SD
Somanath
D000328:Adult; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008296:Malaysia / Q000208:ethnology; D008875:Middle Aged; D048008:TNF Receptor-Associated Factor 3 / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000208:ethnology / Q000235:genetics*
D048008:TNF Receptor-Associated Factor 3; C090656:TRAF3 protein, human
CD40; cancer; cervix; polymorphism