In vivo and in vitro assessment of the biocompatibility and degradation of high-purity Mg anastomotic staples.
Titanium (Ti) staples are not biodegradable, and anastomotic complications related to Ti staples are reported frequently. In the present study, the biocompatibility and degradation behavior of high-purity magnesium (HP Mg) staples with the small intestine were investigated. HP Mg staples did not affect the relative growth rate, cell cycle and apoptosis of primary rectal mucosal epithelial cells (IEC-6) in vitro. At one, two and three days after immersion in intestinal juice, the weight of the 30 rinsed HP Mg staples reduced by 7.5 ± 1.6, 10.6 ± 2.2 and 13.5 ± 2.1 mg, respectively, and those in the Hanks' solution reduced by 3.9 ± 0.8, 6.1 ± 1.2 and 7.1 ± 2.4 mg. Extracts of HP Mg staples were bio-safe for IEC-6, and the corrosion rate of HP staples was faster in the small intestinal juice than in the Hanks' solution. In the in vivo experiments, the small intestine of the minipigs was anastomosed by HP Mg and Ti staples. HP Mg staples neither affected important bio-chemical parameters nor induced serious inflammation or necrosis in the anastomosis tissues. The residual weight of a HP Mg staples (0.81 ± 0.13 mg) was 89.7% of the original weight (9 ± 0.09 mg) one month after surgery. The in vivo corrosion rate for one HP Mg staple was determined to be∼0.007 ± 0.001 mm·month-1. The preliminary results of the biocompatibility and degradation of high-purity Mg anastomotic staples are promising, and further studies will be initiated to study in more detail.
1 Department of Gastroenterology, Wuxi Second Hospital, Nanjing Medical University, Jiangsu, PR China.
Su
S
Qu
2 Department of General Surgery, Wuxi Second Hospital, Nanjing Medical University, Jiangsu, PR China.
Jiazeng
J
Xia
3 Department of General Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, PR China.
Jun
J
Yan
4 State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, PR China.
Hongliu
H
Wu
2 Department of General Surgery, Wuxi Second Hospital, Nanjing Medical University, Jiangsu, PR China.
Hao
H
Wang
2 Department of General Surgery, Wuxi Second Hospital, Nanjing Medical University, Jiangsu, PR China.
Yi
Y
Yi
4 State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, PR China.
Xiaonong
X
Zhang
5 Suzhou Origin Medical Technology Co. Ltd., 2 Haicheng Road, Changshu Economic and Technology Development Zone, Jiangsu, PR China.
Shaoxiang
S
Zhang
4 State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, PR China.
ChangLi
C
Zhao
2 Department of General Surgery, Wuxi Second Hospital, Nanjing Medical University, Jiangsu, PR China.
Yigang
Y
Chen
D020341:Absorbable Implants; D000714:Anastomosis, Surgical / Q000295:instrumentation*; D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage* / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007421:Intestine, Small / Q000601:surgery*; D013537:Sutures; D013552:Swine; D013556:Swine, Miniature; D016896:Treatment Outcome
D001672:Biocompatible Materials
Biocompatibility; anastomosis; degradation; magnesium material; staples