Serum neurofilament is associated with progression of brain atrophy and disability in early MS.
OBJECTIVE
To investigate a potential effect of riluzole on serum neurofilaments (Nf) compared to placebo and the relationship between longitudinal clinical and MRI outcomes and serum Nf levels.


METHODS
Serum samples were obtained from participants enrolled in a randomized double-blind trial of neuroprotection with riluzole vs placebo as an add-on to weekly interferon-β (IFN-β)-1a IM initiated 3 months after randomization. Nf measurements were performed by ELISA and electrochemiluminescence immunoassay.


RESULTS
Longitudinal serum samples were available from 22 riluzole and 20 placebo participants over 24 months. There was no observed treatment effect with riluzole. Nf light chain (NfL) levels decreased over time (p= 0.007 at 24 months), whereas the Nf heavy chain was unchanged (p= 0.997). Changes in NfL were correlated with EDSS change (p= 0.009) and neuropsychological outcomes. Brain volume decreased more rapidly in patients with high baseline NfL (p= 0.05 at 12 months andp= 0.008 at 24 months) and this relationship became stronger at 24 months (p= 0.024 for interaction). Higher and increasing NfL predicted higher number of gadolinium-enhancing lesions (p< 0.001 for both).


CONCLUSIONS
Our findings support the potential value of serum NfL as a marker of neuroaxonal injury in early multiple sclerosis. Its reduction over time could represent regression to the mean, or a possible treatment effect of IFN-β-1a. The association with whole brain atrophy and the formation of acute white matter lesions has relevant implications to use serum NfL as a noninvasive biomarker of the overall consequences of brain damage and ongoing disease activity.


BACKGROUND
NCT00501943.
From Neurologic Clinic and Policlinic (J.K., C.B., O.Y.), Departments of Medicine, Clinical Research, and Biomedicine, University Hospital Basel, University of Basel, Switzerland; Department of Neurology (B.N., E.W.), University of California San Francisco; Department of Neuroimmunology (D.G.), Queen Square, Institute of Neurology, London; independent statistician (S.M.), Haddenham, Bucks, UK; Department of Neurology (D.P.), University of Southern California, Los Angeles; and Department of Neuroscience and Trauma (G.G., S.G.), Queen Mary University of London, UK. jens.kuhle@usb.ch.
Jens
J
Kuhle
From Neurologic Clinic and Policlinic (J.K., C.B., O.Y.), Departments of Medicine, Clinical Research, and Biomedicine, University Hospital Basel, University of Basel, Switzerland; Department of Neurology (B.N., E.W.), University of California San Francisco; Department of Neuroimmunology (D.G.), Queen Square, Institute of Neurology, London; independent statistician (S.M.), Haddenham, Bucks, UK; Department of Neurology (D.P.), University of Southern California, Los Angeles; and Department of Neuroscience and Trauma (G.G., S.G.), Queen Mary University of London, UK.
Bardia
B
Nourbakhsh
From Neurologic Clinic and Policlinic (J.K., C.B., O.Y.), Departments of Medicine, Clinical Research, and Biomedicine, University Hospital Basel, University of Basel, Switzerland; Department of Neurology (B.N., E.W.), University of California San Francisco; Department of Neuroimmunology (D.G.), Queen Square, Institute of Neurology, London; independent statistician (S.M.), Haddenham, Bucks, UK; Department of Neurology (D.P.), University of Southern California, Los Angeles; and Department of Neuroscience and Trauma (G.G., S.G.), Queen Mary University of London, UK.
Donna
D
Grant
From Neurologic Clinic and Policlinic (J.K., C.B., O.Y.), Departments of Medicine, Clinical Research, and Biomedicine, University Hospital Basel, University of Basel, Switzerland; Department of Neurology (B.N., E.W.), University of California San Francisco; Department of Neuroimmunology (D.G.), Queen Square, Institute of Neurology, London; independent statistician (S.M.), Haddenham, Bucks, UK; Department of Neurology (D.P.), University of Southern California, Los Angeles; and Department of Neuroscience and Trauma (G.G., S.G.), Queen Mary University of London, UK.
Steve
S
Morant
From Neurologic Clinic and Policlinic (J.K., C.B., O.Y.), Departments of Medicine, Clinical Research, and Biomedicine, University Hospital Basel, University of Basel, Switzerland; Department of Neurology (B.N., E.W.), University of California San Francisco; Department of Neuroimmunology (D.G.), Queen Square, Institute of Neurology, London; independent statistician (S.M.), Haddenham, Bucks, UK; Department of Neurology (D.P.), University of Southern California, Los Angeles; and Department of Neuroscience and Trauma (G.G., S.G.), Queen Mary University of London, UK.
Christian
C
Barro
From Neurologic Clinic and Policlinic (J.K., C.B., O.Y.), Departments of Medicine, Clinical Research, and Biomedicine, University Hospital Basel, University of Basel, Switzerland; Department of Neurology (B.N., E.W.), University of California San Francisco; Department of Neuroimmunology (D.G.), Queen Square, Institute of Neurology, London; independent statistician (S.M.), Haddenham, Bucks, UK; Department of Neurology (D.P.), University of Southern California, Los Angeles; and Department of Neuroscience and Trauma (G.G., S.G.), Queen Mary University of London, UK.
Özgür
Ö
Yaldizli
From Neurologic Clinic and Policlinic (J.K., C.B., O.Y.), Departments of Medicine, Clinical Research, and Biomedicine, University Hospital Basel, University of Basel, Switzerland; Department of Neurology (B.N., E.W.), University of California San Francisco; Department of Neuroimmunology (D.G.), Queen Square, Institute of Neurology, London; independent statistician (S.M.), Haddenham, Bucks, UK; Department of Neurology (D.P.), University of Southern California, Los Angeles; and Department of Neuroscience and Trauma (G.G., S.G.), Queen Mary University of London, UK.
Daniel
D
Pelletier
From Neurologic Clinic and Policlinic (J.K., C.B., O.Y.), Departments of Medicine, Clinical Research, and Biomedicine, University Hospital Basel, University of Basel, Switzerland; Department of Neurology (B.N., E.W.), University of California San Francisco; Department of Neuroimmunology (D.G.), Queen Square, Institute of Neurology, London; independent statistician (S.M.), Haddenham, Bucks, UK; Department of Neurology (D.P.), University of Southern California, Los Angeles; and Department of Neuroscience and Trauma (G.G., S.G.), Queen Mary University of London, UK.
Gavin
G
Giovannoni
From Neurologic Clinic and Policlinic (J.K., C.B., O.Y.), Departments of Medicine, Clinical Research, and Biomedicine, University Hospital Basel, University of Basel, Switzerland; Department of Neurology (B.N., E.W.), University of California San Francisco; Department of Neuroimmunology (D.G.), Queen Square, Institute of Neurology, London; independent statistician (S.M.), Haddenham, Bucks, UK; Department of Neurology (D.P.), University of Southern California, Los Angeles; and Department of Neuroscience and Trauma (G.G., S.G.), Queen Mary University of London, UK.
Emmanuelle
E
Waubant
From Neurologic Clinic and Policlinic (J.K., C.B., O.Y.), Departments of Medicine, Clinical Research, and Biomedicine, University Hospital Basel, University of Basel, Switzerland; Department of Neurology (B.N., E.W.), University of California San Francisco; Department of Neuroimmunology (D.G.), Queen Square, Institute of Neurology, London; independent statistician (S.M.), Haddenham, Bucks, UK; Department of Neurology (D.P.), University of Southern California, Los Angeles; and Department of Neuroscience and Trauma (G.G., S.G.), Queen Mary University of London, UK.
Sharmilee
S
Gnanapavan
D000276:Adjuvants, Immunologic / Q000627:therapeutic use; D000328:Adult; D001284:Atrophy; D015415:Biomarkers / Q000097:blood; D001921:Brain / Q000000981:diagnostic imaging* / Q000187:drug effects; D004185:Disability Evaluation; D018450:Disease Progression; D004359:Drug Therapy, Combination; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D000068556:Interferon beta-1a / Q000627:therapeutic use; D008279:Magnetic Resonance Imaging; D008297:Male; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000000981:diagnostic imaging* / Q000188:drug therapy / Q000503:physiopathology* / Q000523:psychology; D016900:Neurofilament Proteins / Q000097:blood*; D018696:Neuroprotective Agents / Q000627:therapeutic use; D009483:Neuropsychological Tests; D009929:Organ Size; D019782:Riluzole / Q000627:therapeutic use; D016896:Treatment Outcome
D000276:Adjuvants, Immunologic; D015415:Biomarkers; D016900:Neurofilament Proteins; D018696:Neuroprotective Agents; C044882:neurofilament protein L; C051774:neurofilament protein H; D019782:Riluzole; D000068556:Interferon beta-1a