Single-shot quantitative phase microscopy with color-multiplexed differential phase contrast (cDPC).
We present a new technique for quantitative phase and amplitude microscopy from a single color image with coded illumination. Our system consists of a commercial brightfield microscope with one hardware modification-an inexpensive 3D printed condenser insert. The method, color-multiplexed Differential Phase Contrast (cDPC), is a single-shot variant of Differential Phase Contrast (DPC), which recovers the phase of a sample from images with asymmetric illumination. We employ partially coherent illumination to achieve resolution corresponding to 2× the objective NA. Quantitative phase can then be used to synthesize DIC and phase contrast images or extract shape and density. We demonstrate amplitude and phase recovery at camera-limited frame rates (50 fps) for various in vitro cell samples and c. elegans in a micro-fluidic channel.
Graduate Group in Applied Science and Technology, University of California, Berkeley, United States of America.
Zachary F
http://orcid.org/0000-0002-8293-6798
ZF
Phillips
Dept. of Electrical Engineering and Computer Sciences, University of California, Berkeley, United States of America.
Michael
M
Chen
Graduate Group in Applied Science and Technology, University of California, Berkeley, United States of America.|Dept. of Electrical Engineering and Computer Sciences, University of California, Berkeley, United States of America.
Laura
http://orcid.org/0000-0003-1243-2356
L
Waller
D000818:Animals; D017173:Caenorhabditis elegans / Q000648:ultrastructure; D002138:Calibration; D003116:Color; D007091:Image Processing, Computer-Assisted / Q000379:methods; D044085:Microfluidics / Q000295:instrumentation; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation / Q000379:methods*; D008962:Models, Theoretical