Development of class model based on blood biochemical parameters as a diagnostic tool of PSE meat.
A fast, sensitive and effective method based on the blood biochemical parameters for the detection of PSE meat was developed in this study. A total of 200 pigs were slaughtered in the same slaughterhouse. Meat quality was evaluated by measuring pH, electrical conductivity and color at 45min, 2h and 24h after slaughtering in M. longissimus thoracis et lumborum (LD). Blood biochemical parameters were determined in blood samples collected during carcass bleeding. Principal component analysis (PCA) biplot showed that high levels of exsanguination Creatine Kinase, Lactate Dehydrogenase, Aspertate aminotransferase, blood glucose and lactate were associated with the PSE meat, and the five biochemical parameters were found to be good indicators of PSE meat Discriminant function analysis (DFA) was able to clearly identify PSE meat using the five biochemical parameters as input data, and the class model is an effective diagnostic tool in pigs which can be used to detect the PSE meat and reduce economic loss for the company.
Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, PR China.
Daofeng
D
Qu
Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, PR China.
Xu
X
Zhou
Zhejiang Qinglian Food Co., Ltd., Jiaxing 314300, PR China.
Feng
F
Yang
Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, PR China.
Shiyi
S
Tian
Zhejiang Qinglian Food Co., Ltd., Jiaxing 314300, PR China.
Xiaojun
X
Zhang
Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, PR China.
Lin
L
Ma
Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, PR China. Electronic address: jianzhonghan1@163.com.
Jianzhong
J
Han
D000003:Abattoirs; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose / Q000032:analysis; D002681:China; D003402:Creatine Kinase / Q000097:blood; D003433:Crosses, Genetic; D016002:Discriminant Analysis; D004553:Electric Conductivity; D005513:Food Inspection; D063427:Food Quality; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000097:blood; D019344:Lactic Acid / Q000097:blood; D008460:Meat / Q000032:analysis* / Q000145:classification; D008462:Meat-Packing Industry / Q000379:methods*; D008954:Models, Biological; D018482:Muscle, Skeletal / Q000737:chemistry / Q000254:growth & development*; D010860:Pigments, Biological / Q000032:analysis; D025341:Principal Component Analysis; D034421:Sus scrofa / Q000097:blood / Q000254:growth & development*
D015415:Biomarkers; D001786:Blood Glucose; D010860:Pigments, Biological; D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D003402:Creatine Kinase
Biplot; Blood biochemical parameters; Creatinine (PubChem CID 588); Discriminant analysis; Glucose (PubChem CID 5793); Kalium (PubChem CID 5462222); Lactate (PubChem CID 94386); Natriumion (PubChem CID 923); PCA; PSE meat; Pigs; Total cholesterol (PubChem CID 5997); Triglyceride (PubChem CID 5460048); Urea nitrogen (PubChem CID 1176)