Genetic polymorphism of interleukin-10 (-A592C) among oral cancer with squamous cell carcinoma.
OBJECTIVE
Interleukin-10 (IL-10) is a pleiotropic cytokine with either immunosuppressive or immunostimulative activities. It has been reported that in cancer, the promoter region polymorphism of IL-10 (-A592C) alters both the expression and serum levels of this cytokine. In the present study, we have addressed the question as to whether the single nucleotide polymorphisms (SNPs) at positions -592 A/C in the IL-10 gene promoter, could predispose an individual to oral squamous cell carcinoma (OSCC).


METHODS
We analyzed the genotype of the IL-10 (-A592C) gene, in 250 histopathologically confirmed OSCC patients and similar number of healthy volunteers taken as controls, in an Indian population by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Allele and genotype frequencies were analyzed by the Student's t-test and the chi-squared test, and strength of associations by the odds ratio (OR) with 95% confidence intervals.


RESULTS
The genotype and allele distribution of IL-10 (-A592C) gene polymorphism was significantly different between OSCC cases and controls (genotype AA vs AC: OR 2.87; 95 % CI 1.50-5.48; p=0.0016 and AA vs CC: OR 4.08; 95 % CI 1.98-8.41; p=0.0002). The -592 C alleles were found to be significantly different among OSCC cases and controls (OR: 1.44, 95% CI: 1.12-1.85, p<0.0051).


CONCLUSIONS
The IL-10 gene promoter region (-592) A/C polymorphism is significantly associated with reduced risk of OSCC. The OSCC group had a significantly greater frequency of genotype AA as compared to control group.
Department of Anaesthesiology, King George Medical University, UP, Lucknow, India.
Prithvi Kumar
PK
Singh
Department of Biochemistry, King George Medical University, UP, Lucknow, India.
Mohammad Kaleem
MK
Ahmad
Department of Surgical Oncology, King George Medical University, UP, Lucknow, India.
Vijay
V
Kumar
Department of Anaesthesiology, King George Medical University, UP, Lucknow, India.
Rajni
R
Gupta
Department of Anaesthesiology, King George Medical University, UP, Lucknow, India.
Monica
M
Kohli
Department of Microbiology, King George Medical University, UP, Lucknow, India.
Amita
A
Jain
Department of Biochemistry, King George Medical University, UP, Lucknow, India.
Abbas Ali
AA
Mahdi
Department of Anaesthesiology, King George Medical University, UP, Lucknow, India.
Jaishri
J
Bogra
Department of Anaesthesiology, King George Medical University, UP, Lucknow, India. Electronic address: girishkgmu123@gmail.com.
Girish
G
Chandra
D000328:Adult; D000428:Alcohol Drinking / Q000009:adverse effects; D000483:Alleles; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007194:India; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012307:Risk Factors; D014029:Tobacco Use Disorder / Q000150:complications
C508609:IL10 protein, human; D016753:Interleukin-10
Cytokines; Genotype; Immunosuppressive; Interleukin; Oral squamous cell carcinoma