Effects of several in-feed antibiotic combinations on the abundance and diversity of fecal microbes in weaned pigs.
To characterize the effects of several subtherapeutic antibiotic combinations on the abundance and diversity of fecal microbes, 400 weaned pigs were selected and randomly assigned to 8 groups, where they were continuously fed different antibiotic combinations for 28 days. Then, a total of 48 pigs were randomly selected to collect feces samples for DNA extraction and 16S rRNA high-throughput sequencing. Compared with that of pigs without antibiotic administration, the diversities of fecal microbes were decreased in the pigs fed chlortetracycline (CTC), olaquindox (OLA), and either enramycin (ER) or virginiamycin (VIR), and the relative abundances of members of the phylum Bacteroidetes and the genus Prevotella were increased. Compared with that of pigs without antibiotic administration, the relative abundances of members of the phylum Proteobacteria and the genus Succinivibrio were decreased in the pigs fed CTC, a mixture of zinc bacitracin (ZB) + colistin sulfate (COL), a mixture of CTC+ZB+COL, a mixture of CTC+OLA, or a mixture of CTC+OLA+ER. α-Diversity and β-diversity were decreased (P < 0.05) in the pigs fed ZB+COL or a mixture of CTC+OLA+ER or CTC+OLA+VIR. This study provides experimental data to deepen our understanding on the effects of antibiotic combinations on intestinal microbes.
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.|College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
Huizhi
H
Li
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.|College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
Tingting
T
Liang
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.|College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
Qingpo
Q
Chu
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.|College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
Feilong
F
Xu
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.|College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
Yuan
Y
Li
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.|College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
Lingling
L
Fu
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.|College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
Bo
B
Zhou
D000821:Animal Feed; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology*; D044822:Biodiversity; D004359:Drug Therapy, Combination; D005243:Feces / Q000382:microbiology; D012336:RNA, Ribosomal, 16S; D013552:Swine
D000900:Anti-Bacterial Agents; D012336:RNA, Ribosomal, 16S
16S rRNA gene; Illumina; antibiotic combination; combinaison d’antibiotiques; fecal microbes; gène de l’ARNr 16S; microbes fécaux; pig; porc