Mass Spectrometric Analysis of Non-canonical Cyclic Nucleotides.
Contemporary investigations regarding the (patho)physiological roles of the non-canonical cyclic nucleoside monophosphates (cNMP) cytidine 3',5'-cyclic monophosphate (cCMP) and uridine 3',5'-cyclic monophosphate (cUMP) have been hampered by the lack of highly specific and sensitive analytic methods for these analytes. In addition, the existence of 2',3'-cNMP besides 3',5'-cNMP has been described recently. HPLC coupled with tandem mass spectrometry (HPLC-MS/MS) is the method of choice for identification and quantification of low-molecular weight endogenous metabolites. In this chapter, recommendations for an HPLC-MS/MS method for 3',5'- and 2',3'-cNMP are summarized.
Institute of Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany. baehre.heike@mh-hannover.de.|Research Core Unit Metabolomics, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany. baehre.heike@mh-hannover.de.
Heike
H
Bähre
Research Core Unit Metabolomics, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany.
Volkhard
V
Kaever
D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D006801:Humans; D051379:Mice; D009712:Nucleotides, Cyclic / Q000032:analysis*; D012015:Reference Standards; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards
D009712:Nucleotides, Cyclic
2′,3′-cNMPs; 3′,5′-cNMPs; Liquid chromatography; Mass spectrometry