Arabidopsis RabF1 (ARA6) Is Involved in Salt Stress and Dark-Induced Senescence (DIS).
Arabidopsis small GTPase RabF1 (ARA6) functions in endosomal vesicle transport and may play a crucial role in recycling and degradation of molecules, thus involved in stress responses. Here we have reported that complementary overexpression lines RabF1OE (overexpression), GTPase mutants RabF1Q93L (constitutively active) and RabF1S47N (dominant negative) lines show longer root growth than wild-type, rabF1 knockout and N-myristoylation deletion (Δ1-29, N-terminus) complementary overexpression mutant plants under salt induced stress, which indicates that N-myristoylation of RabF1 is indispensable for salt tolerance. Moreover, RabF1 is highly expressed during senescence and RabF1OE lines were more tolerant of dark-induced senescence (DIS) than wild-type and rabF1.
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210014, China. karim.sazzad@gmail.com.|Department of Biological and Environmental Sciences, University of Gothenburg, Box 461, Gothenburg SE40530, Sweden. karim.sazzad@gmail.com.
Congfei
C
Yin
Department of Biological and Environmental Sciences, University of Gothenburg, Box 461, Gothenburg SE40530, Sweden. karim.sazzad@gmail.com.
Sazzad
S
Karim
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210014, China. hszhang@njau.edu.cn.
Hongsheng
H
Zhang
Department of Biological and Environmental Sciences, University of Gothenburg, Box 461, Gothenburg SE40530, Sweden. henrik.aronsson@bioenv.gu.se.
Henrik
H
Aronsson
D000375:Aging; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D003624:Darkness; D018506:Gene Expression Regulation, Plant; D018515:Plant Leaves; D030821:Plants, Genetically Modified; D055049:Salt Tolerance / Q000235:genetics*; D013312:Stress, Physiological / Q000235:genetics*; D020691:rab GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism
D029681:Arabidopsis Proteins; C436706:Ara6 protein, Arabidopsis; D020691:rab GTP-Binding Proteins
ARA6; RabF1; chloroplast; salt stress; senescence; vesicle