Structural characterization of the PTS IIA and IIB proteins associated with pneumococcal fucose utilization.
Streptococcus pneumoniae harbors a significant number of transporters, including phosphotransferase (PTS) systems, allowing the bacterium to utilize a number of different carbohydrates for metabolic and other purposes. The genes encoding for one PTS transport system in particular (EIIfuc) are found within a fucose utilization operon in S. pneumoniae TIGR4. Here, we report the three-dimensional structures of IIAfucand IIBfucproviding evidence that this PTS system belongs to the EIImanfamily. Additionally, the predicted metabolic pathway for this distinctive fucose utilization system suggests that EIIfuctransports the H-disaccharide blood group antigen, which would represent a novel PTS transporter specificity. Proteins 2017; 85:963-968. © 2016 Wiley Periodicals, Inc.
Biochemistry and Microbiology, University of Victoria, PO Box 3055 STN CSC, Victoria, British Columbia, V8W 3P6, Canada.
Melanie A
0000-0003-1485-1378
MA
Higgins
Biochemistry and Microbiology, University of Victoria, PO Box 3055 STN CSC, Victoria, British Columbia, V8W 3P6, Canada.
Aileen M
AM
Hamilton
Biochemistry and Microbiology, University of Victoria, PO Box 3055 STN CSC, Victoria, British Columbia, V8W 3P6, Canada.
Alisdair B
AB
Boraston
D000017:ABO Blood-Group System / Q000737:chemistry* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D001692:Biological Transport; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005643:Fucose / Q000737:chemistry* / Q000378:metabolism; D015870:Gene Expression; D026901:Membrane Transport Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics; D008958:Models, Molecular; D009876:Operon; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D000072756:Protein Conformation, alpha-Helical; D000072757:Protein Conformation, beta-Strand; D054730:Protein Interaction Domains and Motifs; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013296:Streptococcus pneumoniae / Q000737:chemistry* / Q000378:metabolism
D000017:ABO Blood-Group System; D001426:Bacterial Proteins; C102547:H1 blood group antigen; D026901:Membrane Transport Proteins; D020033:Protein Isoforms; D011994:Recombinant Proteins; D005643:Fucose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System
Streptococcus pneumoniae; carbohydrate transport; crystal structure; fucose metabolism; phosphoenolpyruvate:sugar phosphotransferase system