Transcriptomic Analyses of Ascorbic Acid and Carotenoid Metabolites Influenced by Root Restriction during Grape Berry Development and Ripening.
Ascorbic acid (AsA) and carotenoids are recognized as crucial metabolites for various biological processes in plants. The contents of AsA and carotenoids in fruits are influenced by external environmental stimuli, such as water, temperature, light, and hormones. However, it is still not clear whether it can be affected by root restriction (RR) treatment. In this study, "Summer Black" grape berries (Vitis vinifera × V. labrusca) under RR and control treatments during development and ripening were used as materials. The results showed that RR significantly increased the contents of AsA, and the transcript VIT_08s0040g03150 related to AsA recycling pathways may be the main regulator for AsA. Similarly, the contents of most of the carotenoids at the earlier stages significantly increased by RR; the enzyme crtB encoded by VIT_12s0028g00960 and the enzyme crtZ encoded by VIT_02s0025g00240 and VIT_16s0050g01090 were inferred to play major roles in the carotenoid metabolic pathways.
Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University , Zijingang Campus, Hangzhou 310058, People's Republic of China.
Feng
http://orcid.org/0000-0001-7504-8621
F
Leng
Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University , Zijingang Campus, Hangzhou 310058, People's Republic of China.
Dandan
D
Tang
Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University , Zijingang Campus, Hangzhou 310058, People's Republic of China.|Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Opening Laboratory of Agricultural Products Processing and Quality Control, Ministry of Agriculture , Beijing 100193, People's Republic of China.
Qiong
Q
Lin
Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University , Zijingang Campus, Hangzhou 310058, People's Republic of China.|Taizhou Academy of Agricultural Sciences , Linhai 317000, People's Republic of China.
Jinping
J
Cao
Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University , Zijingang Campus, Hangzhou 310058, People's Republic of China.
Di
D
Wu
School of Agriculture and Biology, Shanghai Jiao Tong University , Shanghai 200240, People's Republic of China.
Shiping
S
Wang
Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University , Zijingang Campus, Hangzhou 310058, People's Republic of China.
Chongde
C
Sun
D001205:Ascorbic Acid / Q000378:metabolism*; D002338:Carotenoids / Q000378:metabolism*; D005638:Fruit / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D053858:Metabolic Networks and Pathways; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D027843:Vitis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
D010940:Plant Proteins; D002338:Carotenoids; D001205:Ascorbic Acid
RNA-Seq; ascorbic acid; carotenoid; grape berry; root restriction