Effects of alkaline treatment for fibroblastic adhesion on titanium.
BACKGROUND
The surface energy of titanium (Ti) implants is very important when determining hydrophilicity or hydrophobicity, which is vital in osseointegration. The purpose of this study was to determine how Ti plates with an alkaline treatment (NaOH) affect the adhesion and proliferation of human periodontal ligament fibroblasts (HPLF).


METHODS
In vitroexperimental study was carried out. Type 1 commercially pure Ti plates were analyzed with atomic force microscopy to evaluate surface roughness. The plates were treated ultrasonically with NaOH at 5 M (pH 13.7) for 45 s. HPLF previously established from periodontal tissue was inoculated on the treated Ti plates. The adhered and proliferated viable cell numbers were determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method for 60 min and 24 h, respectively. The data were analyzed using Kruskal-Wallis tests and multiple comparisons of the Mann-Whitney U-test,P value was fixed at 0.05.


RESULTS
The mean roughness values equaled 0.04 μm with an almost flat surface and some grooves. The alkaline treatment of Ti plates caused significantly (P< 0.05) more pronounced HPLF adhesion and proliferation compared to untreated Ti plates.


CONCLUSIONS
The treatment of Ti plates with NaOH enhances cell adhesion and the proliferation of HPLF cells. Clinically, the alkaline treatment of Ti-based implants could be an option to improve and accelerate osseointegration.
Interdisciplinary Research Laboratory, Nanostructures and Biomaterials Area, Leon, Mexico.
Miryam
M
Cuellar-Flores
Interdisciplinary Research Laboratory, Nanostructures and Biomaterials Area, Leon, Mexico.
Laura Susana
LS
Acosta-Torres
Oral and Maxillofacial Surgery Area, Unit Leon, National Autonomous University of Mexico, Leon, Mexico.
Omar
O
Martínez-Alvarez
Oral Public Health, National School of Higher Education, Unit Leon, National Autonomous University of Mexico, Leon, Mexico.
Benjamin
B
Sánchez-Trocino
Politecnich University of Guanajuato, Cortazar, Gto, Mexico.
Javier
J
de la Fuente-Hernández
Faculty of Dentistry, Autonomous University State of Mexico, Toluca, Mexico.
Rigoberto
R
Garcia-Garduño
Interdisciplinary Research Laboratory, Nanostructures and Biomaterials Area, Leon, Mexico.
Rene
R
Garcia-Contreras
Cells; periodontal ligament; sodium hydroxide; titanium; wettability