Maresin 1 Mitigates High Glucose-Induced Mouse Glomerular Mesangial Cell Injury by Inhibiting Inflammation and Fibrosis.
Background. Inflammation and fibrosis are the important pathophysiologic processes in diabetic nephropathy (DN). Maresin 1 is a potential anti-inflammatory lipid mediator, which has displayed powerful proresolving activities.Aim. We determine whether maresin 1 has protective effect on mouse glomerular mesangial cells (GMCs) induced by high glucose.Methods. We cultured GMCs stimulated by high glucose and categorized as follows: normal glucose group (5.6 mmol/L), high glucose group (30 mmol/L), mannitol group, maresin 1 intervention group (1, 10, and 100 nmol/L), maresin 1 and normal glucose group, and the N-acetylcysteine (NAC) intervention group (10μmol/L NAC). After 24 h, the expression of ROS, NLRP3, caspase-1, procaspase-1, IL-1β, and pro-IL-1βwas detected by western-blot, RT-PCR, and immunofluorescence. After 48 h, the expression of TGF-β1 and FN was detected by RT-PCR and ELISA.Results. Compared with normal glucose group, the expression of ROS, NLRP3, caspase-1, IL-1β, TGF-β1, and FN increased in high glucose group (P< 0.05), but it decreased after the treatment of maresin 1 in different concentrations. On the contrary, the expression of procaspase-1 and pro-IL-1βprotein was restrained by high glucose and enhanced by maresin 1 in a dose-dependent manner (P< 0.05).Conclusion. Maresin 1 can inhibit NLRP3 inflammasome, TGF-β1, and FN in GMCs; it may have protective effect on DN by mitigating the inflammation and early fibrosis.
Endocrinology Department, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China; The Graduate School of Southwest Medical University, Luzhou, Sichuan 646000, China.
Shi
S
Tang
State Key Laboratory of Quality Research in Chinese Medicine, Faculty of Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau.
Chenlin
0000-0003-2464-9325
C
Gao
Endocrinology Department, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China.
Yang
Y
Long
Endocrinology Department, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China.
Wei
W
Huang
The Graduate School of Southwest Medical University, Luzhou, Sichuan 646000, China.
Jiao
J
Chen
The Graduate School of Southwest Medical University, Luzhou, Sichuan 646000, China.
Fang
F
Fan
The Graduate School of Southwest Medical University, Luzhou, Sichuan 646000, China.
Chunxia
C
Jiang
Endocrinology Department, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China.
Yong
0000-0002-9534-6252
Y
Xu
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D020170:Caspase 1 / Q000378:metabolism; D002478:Cells, Cultured; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D005355:Fibrosis / Q000276:immunology* / Q000378:metabolism; D005947:Glucose / Q000633:toxicity*; D007249:Inflammation / Q000276:immunology* / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D050527:Mesangial Cells / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects
C535211:7,14-dihydroxydocosa-4,8,10,12,16,19-hexaenoic acid; D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D017382:Reactive Oxygen Species; D004281:Docosahexaenoic Acids; D020170:Caspase 1; D005947:Glucose