Thermal dissociation of ions limits the degree of the gas-phase H/D exchange at the atmospheric pressure.
We present the application of the extended desolvating capillaries for increasing the degree of the gas-phase hydrogen/deuterium exchange reaction at atmospheric pressure. The use of the extended capillaries results in the increase of the time that ions spend in the high pressure region, what leads to the significant improvement of the efficiency of the reaction. For the small protein ubiquitin, it was observed that for the same temperature, the number of exchanges increases with the decrease of the charge state so that the lowest charge state can exchange twice the number of hydrogen than the highest one. With the increase of the temperature, the difference decreases, and eventually, the number of exchanges equalizes for all charge states. The value of this temperature and the corresponding number of exchanges depend on the geometric parameters of the capillary. Further increase of the temperature leads to the thermal dissociation of the protein ion. The observed b/y fragments are identical to those produced by collision-induced dissociation performed in the ion trap. Copyright © 2017 John Wiley & Sons, Ltd.
Skolkovo Institute of Science and Technology, Novaya St., 100, Skolkovo, 143025, Russia.|Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Leninskij pr. 38 k.2, 119334, Moscow, Russia.|Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, Kosygina st. 4, 119334, Moscow, Russia.|Moscow Institute of Physics and Technology, 141700 Dolgoprudnyi, Moscow Region, Russia.
Y
0000-0002-1955-9336
Y
Kostyukevich
Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Leninskij pr. 38 k.2, 119334, Moscow, Russia.|Moscow Institute of Physics and Technology, 141700 Dolgoprudnyi, Moscow Region, Russia.
A
A
Kononikhin
Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, Kosygina st. 4, 119334, Moscow, Russia.|Moscow Institute of Physics and Technology, 141700 Dolgoprudnyi, Moscow Region, Russia.
I
I
Popov
Skolkovo Institute of Science and Technology, Novaya St., 100, Skolkovo, 143025, Russia.|Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Leninskij pr. 38 k.2, 119334, Moscow, Russia.|Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, Kosygina st. 4, 119334, Moscow, Russia.|Moscow Institute of Physics and Technology, 141700 Dolgoprudnyi, Moscow Region, Russia.
E
E
Nikolaev
ESI; FT ICR; dissolved organic matter; fragmentation; isotopic exchange; proteins