Acute exercise activates p38 MAPK and increases the expression of telomere-protective genes in cardiac muscle.
What is the central question of this study? A positive association between telomere length and exercise training has been shown in cardiac tissue of mice. It is currently unknown how each bout of exercise influences telomere-length-regulating proteins. We sought to determine how a bout of exercise altered the expression of telomere-length-regulating genes and a related signalling pathway in cardiac tissue of mice. What is the main finding and its importance? Acute exercise altered the expression of telomere-length-regulating genes in cardiac tissue and might be related to altered mitogen-activated protein kinase signalling. These findings are important in understanding how exercise provides a cardioprotective phenotype with ageing. Age is the greatest risk factor for cardiovascular disease. Telomere length is shorter in the hearts of aged mice compared with young mice, and short telomere length has been associated with an increased risk of cardiovascular disease. One year of voluntary wheel-running exercise attenuates the age-associated loss of telomere length and results in altered gene expression of telomere-length-maintaining and genome-stabilizing proteins in heart tissue of mice. Understanding the early adaptive response of the heart to an endurance exercise bout is paramount to understanding the impact of endurance exercise on heart tissue and cells. To this end, we studied mice before (BL), immediately after (TP1) and 1 h after a treadmill running bout (TP2). We measured the changes in expression of telomere-related genes (shelterin components), DNA-damage-sensing (p53 and Chk2) and DNA-repair genes (Ku70 and Ku80) and mitogen-activated protein kinase (MAPK) signalling. The TP1 animals had increased TRF1 and TRF2 protein and mRNA levels, greater expression of DNA-repair and -response genes (Chk2 and Ku80) and greater protein content of phosphorylated p38 MAPK compared with both BL and TP2 animals. These data provide insights into how physiological stressors remodel the heart tissue and how an early adaptive response mediated by exercise may be maintaining telomere length and/or stabilizing the heart genome through the upregulation of telomere-protective genes.
Department of Kinesiology, School of Public Health, University of Maryland at College Park, College Park, MD, USA.|Department of Cell Biology, The University of Texas Southwestern Medical Center, Dallas, TX, USA.
Andrew T
AT
Ludlow
Department of Kinesiology, School of Public Health, University of Maryland at College Park, College Park, MD, USA.|Kinesiology Graduate Program, Catholic University of Brasilia, Brasilia, Brazil.
Laila
L
Gratidão
Department of Kinesiology, School of Public Health, University of Maryland at College Park, College Park, MD, USA.|Department of Applied Physiology, Southern Methodist University, Dallas, TX, USA.
Lindsay W
LW
Ludlow
Department of Physiology, Brody School of Medicine, East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, NC, USA.
Espen E
EE
Spangenburg
Department of Kinesiology, School of Public Health, University of Maryland at College Park, College Park, MD, USA.
Stephen M
0000-0002-7841-3695
SM
Roth
D000375:Aging / Q000235:genetics / Q000378:metabolism; D000818:Animals; D004260:DNA Repair / Q000235:genetics; D005260:Female; D015870:Gene Expression / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D010766:Phosphorylation / Q000235:genetics; D010805:Physical Conditioning, Animal / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D012420:Running / Q000502:physiology; D016615:Telomere / Q000235:genetics*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
D012333:RNA, Messenger; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases
gene expression; telomerase; telomere