SSR-enriched genetic linkage maps of bermudagrass (Cynodon dactylon × transvaalensis), and their comparison with allied plant genomes.
We report SSR-enriched genetic maps of bermudagrass that: (1) reveal partial residual polysomic inheritance in the tetraploid species, and (2) provide insights into the evolution of chloridoid genomes. This study describes genetic linkage maps of two bermudagrass species, Cynodon dactylon (T89) and Cynodon transvaalensis (T574), that integrate heterologous microsatellite markers from sugarcane into frameworks built with single-dose restriction fragments (SDRFs). A maximum likelihood approach was used to construct two separate parental maps from a population of 110 F1progeny of a cross between the two parents. The T89 map is based on 291 loci on 34 cosegregating groups (CGs), with an average marker spacing of 12.5 cM. The T574 map is based on 125 loci on 14 CGs, with an average marker spacing of 10.7 cM. Six T89 and one T574 CG(s) deviated from disomic inheritance. Furthermore, marker segregation data and linkage phase analysis revealed partial residual polysomic inheritance in T89, suggesting that common bermudagrass is undergoing diploidization following whole genome duplication (WGD). Twenty-six T89 CGs were coalesced into 9 homo(eo)logous linkage groups (LGs), while 12 T574 CGs were assembled into 9 LGs, both putatively representing the basic chromosome complement (x = 9) of the species. Eight T89 and two T574 CGs remain unassigned. The marker composition of bermudagrass ancestral chromosomes was inferred by aligning T89 and T574 homologs, and used in comparisons to sorghum and rice genome sequences based on 108 and 91 significant blast hits, respectively. Two nested chromosome fusions (NCFs) shared by two other chloridoids (i.e., zoysiagrass and finger millet) and at least three independent translocation events were evident during chromosome number reduction from 14 in the polyploid common ancestor of Poaceae to 9 in Cynodon.
Plant Genome Mapping Laboratory, University of Georgia, Athens, GA, 30602, USA.
Sameer
S
Khanal
Plant Genome Mapping Laboratory, University of Georgia, Athens, GA, 30602, USA.|Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon, 34134, South Korea.
Changsoo
C
Kim
Plant Genome Mapping Laboratory, University of Georgia, Athens, GA, 30602, USA.
Susan A
SA
Auckland
Plant Genome Mapping Laboratory, University of Georgia, Athens, GA, 30602, USA.
Lisa K
LK
Rainville
Plant Genome Mapping Laboratory, University of Georgia, Athens, GA, 30602, USA.
Jeevan
J
Adhikari
Department of Crop and Soil Sciences, University of Georgia, Tifton, GA, 31793, USA.
Brian M
BM
Schwartz
Plant Genome Mapping Laboratory, University of Georgia, Athens, GA, 30602, USA. paterson@uga.edu.
Andrew H
AH
Paterson
D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D031744:Cynodon / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D020224:Expressed Sequence Tags; D008040:Genetic Linkage; D018745:Genome, Plant; D040582:Inheritance Patterns; D016013:Likelihood Functions; D018895:Microsatellite Repeats; D012150:Polymorphism, Restriction Fragment Length; D057891:Tetraploidy
D018744:DNA, Plant