Extended and direct evaluation of RT-QuIC assays for Creutzfeldt-Jakob disease diagnosis.
Real-Time Quaking-Induced Conversion (RT-QuIC) testing of human cerebrospinal fluid (CSF) is highly sensitive and specific in discriminating sporadic CJD patients from those without prion disease. Here, using CSF samples from 113 CJD and 64 non-prion disease patients, we provide the first direct and concurrent comparison of our improved RT-QuIC assay to our previous assay, which is similar to those commonly used internationally for CJD diagnosis. This extended comparison demonstrated a ~21% increase in diagnostic sensitivity, a 2-day reduction in average detection time, and 100% specificity.
Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute for Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana.
Bradley R
BR
Groveman
Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute for Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana.
Christina D
CD
Orrú
Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute for Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana.
Andrew G
AG
Hughson
Department of Neurological and Movement Sciences, University of Verona, Verona, Italy.
Matilde
M
Bongianni
Department of Neurological and Movement Sciences, University of Verona, Verona, Italy.
Michele
M
Fiorini
Neurology Unit, Ospedale Maria Vittoria, Torino, Italy.
Daniele
D
Imperiale
Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Rome, Italy.
Anna
A
Ladogana
Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Rome, Italy.
Maurizio
M
Pocchiari
Department of Neurological and Movement Sciences, University of Verona, Verona, Italy.
Gianluigi
G
Zanusso
Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute for Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana.
Byron
B
Caughey