Isolation of Adipose-Derived Stromal Vascular Fraction Cells Using a Novel Point-of-Care Device: Cell Characterization and Review of the Literature.
The GID SVF-1 and GID SVF-2 are closed-system, disposable, scalable cellular isolation devices designed for isolating the human adipose-derived stromal vascular fraction (hSVF) from lipoaspirates at the clinical point of care. The purpose of this study was to characterize the device performance with respect to cell yield and viability of the hSVF, as well as the hSVF purity and cellular composition. Our results demonstrate that adipose-derived hSVF can be safely obtained using both devices and standardized methods, yielding cells that are free of bacterial contaminants as measured by endotoxin levels, Gram stain, and culture. In addition, they produce cellular preparations with compositions consistent with reported values within the peer-reviewed literature using similar methods.
1 Division of Plastic and Reconstructive Surgery, Department of Surgery, College of Medicine, University of Florida , Gainesville, Florida.
James Christian
JC
Brown
1 Division of Plastic and Reconstructive Surgery, Department of Surgery, College of Medicine, University of Florida , Gainesville, Florida.
Hulan
H
Shang
1 Division of Plastic and Reconstructive Surgery, Department of Surgery, College of Medicine, University of Florida , Gainesville, Florida.|2 UF-Shands Medical Laboratory at Rocky Point , Gainesville, Florida.
Ying
Y
Li
1 Division of Plastic and Reconstructive Surgery, Department of Surgery, College of Medicine, University of Florida , Gainesville, Florida.
Ning
N
Yang
1 Division of Plastic and Reconstructive Surgery, Department of Surgery, College of Medicine, University of Florida , Gainesville, Florida.
Nikita
N
Patel
1 Division of Plastic and Reconstructive Surgery, Department of Surgery, College of Medicine, University of Florida , Gainesville, Florida.
Adam J
AJ
Katz
D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D000368:Aged; D002454:Cell Differentiation; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods*; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D015187:Lipectomy; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D019095:Point-of-Care Systems; D055815:Young Adult
adipose; adipose-derived stem cell; adult stem cells; clinical translation; point-of-care isolation; stem cell isolation; stromal vascular fraction