Predicting burst pressure of radiofrequency-induced colorectal anastomosis by bio-impedance measurement.
The present study investigates the relationship between bio-impedance and burst pressure of colorectal anastomosis created by radiofrequency (RF)-induced tissue fusion. Colorectal anastomosis were created with ex vivo porcine colorectal segments, during which 5 levels of compression pressure were applied by a custom-made bipolar prototype, with 5 replicate experiments at each compression pressure. Instant anastomotic tensile strength was assessed by burst pressure. Bio-impedance of fused tissue was measured by Impedance Analyzer across frequency that 100 Hz to 3 MHz. Statistical analysis shows only a weak correlation between bio-impedance modulus and burst pressures at frequency of 445 kHz ([Formula: see text]  =  -0.426, P  =  0.099  >  0.05). In contrast, results demonstrated a highly significant negative correlation between reactance modulus and burst pressures ([Formula: see text]  =  -0.812, P  =  0.000  <  0.05). The decrease in mean reactance modulus with increasing burst pressures was highly significant (P  =  0.019  <  0.05). The observed strong negative correlation between reactance modulus and burst pressures at frequency of 445 kHz indicates that reactance is likely to be a good index for tensile strength of RF-induced colorectal anastomosis, and should be considered for inclusion in a feedback loops in devices design.
Shanghai Institute for Minimally Invasive Therapy, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, People's Republic of China.
Lingxi
L
Zhao
Yu
Y
Zhou
Chengli
C
Song
Zhigang
Z
Wang
Alfred
A
Cuschieri
D000714:Anastomosis, Surgical; D000818:Animals; D003106:Colon / Q000166:cytology / Q000528:radiation effects* / Q000601:surgery*; D017097:Electric Impedance; D011312:Pressure; D011846:Radio Waves; D012007:Rectum / Q000166:cytology / Q000528:radiation effects* / Q000601:surgery*; D013552:Swine