Correlation of Blood T-Cells to Intratumoural Density and Location of CD3+and CD8+T-Cells in Colorectal Cancer.
To test the feasibility of conducting parallel analyses of circulating T-cells in blood and intratumoural T-cells in colorectal cancer. A pre-operative 'liquid biopsy' to determine immune status would facilitate clinical decision-making.



A total of 18 patients with stage I-III colorectal cancer (CRC) were included. Blood was analyzed for T-cell type (CD3+, CD4+and CD8+) and count using flow cytometry. Intratumoural T-cells were stained using immunohistochemistry and quantified by digital pathology. Tumour location was defined as invasive front (IF) or tumour center (TC).



The number of CD3+and CD4+T-cells in pre-surgical blood samples correlated with the number of CD3+T-cells found in the IF (Spearman ϱ=0.558, p<0.05 and 0.598, p<0.01 respectively) and CD3+in the TC (ϱ=0.496, p<0.05, and ϱ=0.637, p<0.01, respectively). A strong correlation was found between CD4+cells in blood and CD8+T-cells found in the TC and IF (ϱ=0.602 and ϱ=0.591, p<0.01).



There is a correlation between blood CD3+and CD4+T-cells and the T-cells found at the TC and IF.
Gastrointestinal Translational Research Unit, Molecular Laboratory, Stavanger University Hospital, Stavanger, Norway.|Centre for Organelle Research (CORE), University of Stavanger, Stavanger, Norway.|Department of Gastrointestinal Surgery, Stavanger University Hospital, Stavanger, Norway.
Hanne R
HR
Hagland
Gastrointestinal Translational Research Unit, Molecular Laboratory, Stavanger University Hospital, Stavanger, Norway.|Department of Pathology, Stavanger University Hospital, Stavanger, Norway.
Dordi
D
Lea
Gastrointestinal Translational Research Unit, Molecular Laboratory, Stavanger University Hospital, Stavanger, Norway.|Department of Gastrointestinal Surgery, Stavanger University Hospital, Stavanger, Norway.
Martin M
MM
Watson
Gastrointestinal Translational Research Unit, Molecular Laboratory, Stavanger University Hospital, Stavanger, Norway ksoreide@mac.com.|Department of Gastrointestinal Surgery, Stavanger University Hospital, Stavanger, Norway.|Department of Clinical Medicine, University of Bergen, Bergen, Norway.
Kjetil
K
Søreide
D000368:Aged; D000369:Aged, 80 and over; D017252:CD3 Complex / Q000276:immunology* / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D003106:Colon / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D015179:Colorectal Neoplasms / Q000097:blood / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D057234:Preoperative Period; D012007:Rectum / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism
D017252:CD3 Complex
CD3+; CD4+; CD8+; Colorectal cancer; T-cells; cancer immune response; liquid biopsies; tumour center; tumour invasive front