Analytic and quality control validation and assessment of field performance of a point-of-care chemistry analyzer for use in the White rhinoceros.
BACKGROUND
A chemistry point-of-care analyzer would be useful for evaluating injured wildlife, particularly White rhinoceros (Ceratotherium simum) that survive poaching attempts. The IDEXX VetTest could be suitable, but species-specific validation, development of a statistical quality control (QC) strategy, and evaluation under field conditions are necessary.


OBJECTIVE
The objectives were to (1) validate the VetTest for the White rhinoceros, (2) perform QC validation on the VetTest and generate a statistical QC strategy, and (3) apply this QC strategy to monitor performance under typical field conditions.


METHODS
Differences between White rhinoceros heparin plasma and serum, short-term imprecision, and reportable range using rhinoceros plasma and long-term imprecision using commercial quality control material (QCM) were assessed against prescribed total allowable error (TEa) for up to 15 analytes. Quality control validation was performed using data from the long-term imprecision study and TEa. A QC strategy using QCM was developed and used to monitor performance under field conditions.


RESULTS
Imprecision was acceptable for all analytes except for ALP, ALT, and AST at low activities. The reportable range for AST and LDH differed from the manufacturer's specifications. Eleven analytes were suitable for statistical QC using the 13srule, 3 using the 2srule; ALP was not suitable. In the field, observed error was < TEafor all 15 analytes and the sigma metric was > 3.0 for 12 analytes.


CONCLUSIONS
The VetTest is suitable for use in the White rhinoceros. Statistical QC is possible for most analytes and useful for evaluation of field performance.
Department of Companion Animal Clinical Studies, Faculty of Veterinary Science, University of Pretoria, Pretoria, South Africa.
Emma H
EH
Hooijberg
Department of Companion Animal Clinical Studies, Faculty of Veterinary Science, University of Pretoria, Pretoria, South Africa.
Gerhard
G
Steenkamp
Department of Companion Animal Clinical Studies, Faculty of Veterinary Science, University of Pretoria, Pretoria, South Africa.
Jacques P
JP
du Preez
Department of Companion Animal Clinical Studies, Faculty of Veterinary Science, University of Pretoria, Pretoria, South Africa.
Amelia
A
Goddard
D000818:Animals; D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000592:standards / Q000662:veterinary*; D003951:Diagnostic Errors / Q000662:veterinary*; D010527:Perissodactyla / Q000097:blood*; D019095:Point-of-Care Systems / Q000592:standards*; D011786:Quality Control; D013045:Species Specificity

Ceratotherium simum
; VetTest; dry chemistry; total allowable error; wildlife