The Use of Lectin Histochemistry for Detecting Apoptotic Cells in the Seminiferous Epithelium.
Lectin histochemistry is commonly used to characterize the pattern of glycoconjugates in cells and tissues. Recent studies show that alterations in these glycoconjugates are associated with the entry of cells into apoptosis. A widely used technique for the detection of apoptotic cell death is TUNEL. In this chapter, we study the sensitivity of both techniques to identify apoptotic cells in the testis of photo-inhibited Syrian hamster.
Department of Cell Biology and Histology, School of Medicine, IMIB-Arrixaca, University of Murcia, Regional Campus of International Excellence, Campus Mare Nostrum, Espinardo, Murcia, 30100, Spain.
Vicente
V
Seco-Rovira
Department of Cell Biology and Histology, School of Medicine, IMIB-Arrixaca, University of Murcia, Regional Campus of International Excellence, Campus Mare Nostrum, Espinardo, Murcia, 30100, Spain.
Ester
E
Beltrán-Frutos
Department of Cell Biology and Histology, School of Medicine, IMIB-Arrixaca, University of Murcia, Regional Campus of International Excellence, Campus Mare Nostrum, Espinardo, Murcia, 30100, Spain.
Jesús
J
Martínez-Hernández
Department of Cell Biology and Histology, School of Medicine, IMIB-Arrixaca, University of Murcia, Regional Campus of International Excellence, Campus Mare Nostrum, Espinardo, Murcia, 30100, Spain.
Concepción
C
Ferrer
Department of Cell Biology and Histology, School of Medicine, IMIB-Arrixaca, University of Murcia, Regional Campus of International Excellence, Campus Mare Nostrum, Espinardo, Murcia, 30100, Spain. bioetica@um.es.
Luis Miguel
LM
Pastor
D000818:Animals; D017209:Apoptosis; D015415:Biomarkers; D006224:Cricetinae; D005854:Germ Cells / Q000378:metabolism; D006001:Glycoconjugates; D006651:Histocytochemistry / Q000379:methods*; D020287:In Situ Nick-End Labeling / Q000379:methods; D037102:Lectins / Q000378:metabolism*; D008297:Male; D012670:Seminiferous Epithelium / Q000378:metabolism*; D012680:Sensitivity and Specificity; D013737:Testis / Q000166:cytology / Q000378:metabolism
D015415:Biomarkers; D006001:Glycoconjugates; D037102:Lectins
Apoptosis; Germ cell; Lectin histochemistry; Sensitivity; Syrian hamster; TUNEL; Testis