Selection of Small Synthetic Antimicrobial Peptides InhibitingXanthomonas citrisubsp.citriCausing Citrus Canker.
Citrus canker disease decreases the fruit quality and yield significantly, furthermore, emerging of streptomycin-resistant pathogens threatens the citrus industry seriously because of a lack of proper control agents. Small synthetic antimicrobial peptides (AMPs) could be a promising alternative. Fourteen hexapeptides were selected by using positional scanning of synthetic peptide combinatorial libraries. Each hexapeptide showed different antimicrobial spectrum againstBacillus,Pseudomonas,Xanthomonas, andCandidaspecies. Intriguingly, BHC10 showed bactericidal activity exclusively onXanthomonas citrisubsp.citri(Xcc), while BHC7 was none-active exclusively against twoPseudomonasspp. at concentration of 100 μg/ml suggesting potential selectivity constrained in hexapeptide frame. Three hexapeptides, BHC02, 06 and 11, showed bactericidal activities against variousXccstrains at concentration of 10 μg/ml. When they were co-infiltrated with pathogens into citrus leaves the disease progress was suppressed significantly. Further study would be needed to confirm the actual disease control capacity of the selected hexapeptides.
Department of Biotechnology, Yeungnam University, Gyengsan 38541, Korea.
Jeahyuk
J
Choi
Department of Biotechnology, Yeungnam University, Gyengsan 38541, Korea.
Euiho
E
Park
International Technology Cooperation Center, Rural Development Administration, Jeonju 54875, Korea.
Se-Weon
SW
Lee
Citrus Research Station, National Institute of Horticultural and Herbal Science, Rural Development Administration, Seogwipo 63607, Korea.
Jae-Wook
JW
Hyun
Department of Biotechnology, Yeungnam University, Gyengsan 38541, Korea.
Kwang-Hyun
KH
Baek
Xanthomonas citri subsp. citri; antimicrobial peptides; bactericidal citrus canker; small synthetic; streptomycin-resistance