Purification, structural characterization and anticoagulant properties of fucosylated chondroitin sulfate isolated from Holothuria mexicana.
A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR,1H and13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG). The backbone of HmG was identified as chondroitin 6-O sulfate, while the major O-4 sulfated fucose branches linked to O-3 position of glucuronic acid in almost every disaccharide unit. The anticoagulant activities of HmG and DHmG were assessed and compared with heparin and low molecular weight heparin. The results indicated that HmG and DHmG both could significantly prolong the activated partial thrombo-plastin time, and the properties were well related to its molecular weight. DHmG showed similar anticoagulant properties to low molecular weight heparin with less bleeding risks, making it a safer anticoagulant drug.
College of Pharmacy, Weifang Medical University, Weifang 261053, China.
Jiaojiao
J
Mou
Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
Cong
C
Wang
College of Pharmacy, Weifang Medical University, Weifang 261053, China.
Wenjing
W
Li
College of Pharmacy, Weifang Medical University, Weifang 261053, China. Electronic address: yangjie@wfmc.edu.cn.
Jie
J
Yang
D000818:Animals; D000925:Anticoagulants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002809:Chondroitin Sulfates / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D047332:Holothuria / Q000737:chemistry*; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D013329:Structure-Activity Relationship
D000925:Anticoagulants; D009005:Monosaccharides; C517150:fucosylated chondroitin sulfate; D002809:Chondroitin Sulfates
Anticoagulant; Fucosylated chondroitin sulfate; Holothuria mexicana; Structural characterization