Estimating survival time of patients with glioblastoma multiforme and characterization of the identified microRNA signatures.
Though glioblastoma multiforme (GBM) is the most frequently occurring brain malignancy in adults, clinical treatment still faces challenges due to poor prognoses and tumor relapses. Recently, microRNAs (miRNAs) have been extensively used with the aim of developing accurate molecular therapies, because of their emerging role in the regulation of cancer-related genes. This work aims to identify the miRNA signatures related to survival of GBM patients for developing molecular therapies.



This work proposes a support vector regression (SVR)-based estimator, called SVR-GBM, to estimate the survival time in patients with GBM using their miRNA expression profiles. SVR-GBM identified 24 out of 470 miRNAs that were significantly associated with survival of GBM patients. SVR-GBM had a mean absolute error of 0.63 years and a correlation coefficient of 0.76 between the real and predicted survival time. The 10 top-ranked miRNAs according to prediction contribution are as follows: hsa-miR-222, hsa-miR-345, hsa-miR-587, hsa-miR-526a, hsa-miR-335, hsa-miR-122, hsa-miR-24, hsa-miR-433, hsa-miR-574 and hsa-miR-320. Biological analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway on the identified miRNAs revealed their influence in GBM cancer.



The proposed SVR-GBM using an optimal feature selection algorithm and an optimized SVR to identify the 24 miRNA signatures associated with survival of GBM patients. These miRNA signatures are helpful to uncover the individual role of miRNAs in GBM prognosis and develop miRNA-based therapies.
Institute of Bioinformatics and Systems Biology, National Chiao Tung University, Hsinchu, Taiwan.
Srinivasulu
S
Yerukala Sathipati
Institute of Bioinformatics and Systems Biology, National Chiao Tung University, Hsinchu, Taiwan.|Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan.
Hui-Ling
HL
Huang
Institute of Bioinformatics and Systems Biology, National Chiao Tung University, Hsinchu, Taiwan. syho@mail.nctu.edu.tw.|Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan. syho@mail.nctu.edu.tw.
Shinn-Ying
SY
Ho
D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality*; D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods; D030561:Databases, Nucleic Acid; D020869:Gene Expression Profiling; D005909:Glioblastoma / Q000235:genetics* / Q000378:metabolism / Q000401:mortality*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D011379:Prognosis; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D015398:Signal Transduction; D059467:Transcriptome
D035683:MicroRNAs; D012333:RNA, Messenger