Comparative analysis of NBS-LRR genes and their response to Aspergillus flavus in Arachis.
Studies have demonstrated that nucleotide-binding site-leucine-rich repeat (NBS-LRR) genes respond to pathogen attack in plants. Characterization of NBS-LRR genes in peanut is not well documented. The newly released whole genome sequences of Arachis duranensis and Arachis ipaënsis have allowed a global analysis of this important gene family in peanut to be conducted. In this study, we identified 393 (AdNBS) and 437 (AiNBS) NBS-LRR genes from A. duranensis and A. ipaënsis, respectively, using bioinformatics approaches. Full-length sequences of 278 AdNBS and 303 AiNBS were identified. Fifty-one orthologous, four AdNBS paralogous, and six AiNBS paralogous gene pairs were predicted. All paralogous gene pairs were located in the same chromosomes, indicating that tandem duplication was the most likely mechanism forming these paralogs. The paralogs mainly underwent purifying selection, but most LRR 8 domains underwent positive selection. More gene clusters were found in A. ipaënsis than in A. duranensis, possibly owing to tandem duplication events occurring more frequently in A. ipaënsis. The expression profile of NBS-LRR genes was different between A. duranensis and A. hypogaea after Aspergillus flavus infection. The up-regulated expression of NBS-LRR in A. duranensis was continuous, while these genes responded to the pathogen temporally in A. hypogaea.
Biotechnology Research Center, Shandong Academy of Agricultural Sciences; Shandong Provincial Key laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, China.
Hui
H
Song
Biotechnology Research Center, Shandong Academy of Agricultural Sciences; Shandong Provincial Key laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, China.
Pengfei
P
Wang
Biotechnology Research Center, Shandong Academy of Agricultural Sciences; Shandong Provincial Key laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, China.|College of Life Science, Shandong Normal University, Jinan, China.
Changsheng
C
Li
Henan Academy of Agricultural Sciences, Zhengzhou, China.
Suoyi
S
Han
Biotechnology Research Center, Shandong Academy of Agricultural Sciences; Shandong Provincial Key laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, China.
Chuanzhi
C
Zhao
Biotechnology Research Center, Shandong Academy of Agricultural Sciences; Shandong Provincial Key laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, China.
Han
H
Xia
Biotechnology Research Center, Shandong Academy of Agricultural Sciences; Shandong Provincial Key laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, China.
Yuping
Y
Bi
Crop Protection and Management Research Unit, USDA-ARS, Tifton, Georgia, United States of America.
Baozhu
B
Guo
Henan Academy of Agricultural Sciences, Zhengzhou, China.
Xinyou
X
Zhang
Biotechnology Research Center, Shandong Academy of Agricultural Sciences; Shandong Provincial Key laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, China.|College of Life Science, Shandong Normal University, Jinan, China.
Xingjun
http://orcid.org/0000-0001-7444-4972
X
Wang
D010367:Arachis / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D001231:Aspergillus flavus / Q000472:pathogenicity*; D019295:Computational Biology; D005810:Multigene Family / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*
D010940:Plant Proteins