Rhamnolipids from Pseudomonas aeruginosa strain W10; as antibiofilm/antibiofouling products for metal protection.
Industrial biofouling-problems associated with the accumulation of microorganisms from flowing water and fluids on processing surfaces can cause severe problems. A Pseudomonas aeruginosa strain W10 was isolated from industrial setting and found to produce predominantly di-rhamnolipids (Rha-Rha-C10-C10) with a yield of around 10 g L-1and a critical micelle concentration (CMC) of 80 mg L-1. P. aeruginosa W10 rhamnolipids were able to disrupt up to 99% of 48 h pre-formed biofilms of the Gram-positive organisms Bacillus licheniformis CAN55, Staphylococcus capitis SH6, and a mixed culture (strains CAN55, SH6, and W10), under static conditions, at concentrations of 0.1, 0.5, and 1 mg ml-1on a stainless steel surface commonly used in industrial process pipelines. CFU measurements and LIVE/DEAD BacLight staining confirmed these observations. Furthermore, a purified di-rhamnolipid fraction was found to be responsible for the microbial inhibition of B. licheniformis strain CAN55. This study provides evidence that rhamnolipids may have valuable applications in preventing biofilms and biofouling in industrial plants and, in a wider context, may also apply to metal medical devices.
Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia.
Alif
A
Chebbi
School of Biomedical Sciences, Ulster University, Coleraine, Northern Ireland, United Kingdom.
Mohamed
M
Elshikh
Biochemical Engineering Research & Process Development Center (BERPDC), CSIR-Institute of Microbial Technology, Chandigarh, India.
Farazul
F
Haque
School of Biomedical Sciences, Ulster University, Coleraine, Northern Ireland, United Kingdom.
Syed
S
Ahmed
School of Biomedical Sciences, Ulster University, Coleraine, Northern Ireland, United Kingdom.
Sara
S
Dobbin
School of Biomedical Sciences, Ulster University, Coleraine, Northern Ireland, United Kingdom.
Roger
R
Marchant
Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia.
Sami
S
Sayadi
Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia.
Mohamed
M
Chamkha
School of Biomedical Sciences, Ulster University, Coleraine, Northern Ireland, United Kingdom.
Ibrahim M
IM
Banat
D000890:Anti-Infective Agents / Q000494:pharmacology; D000069976:Bacillus licheniformis / Q000187:drug effects / Q000502:physiology; D018441:Biofilms / Q000187:drug effects*; D057219:Biofouling / Q000517:prevention & control*; D006017:Glycolipids / Q000737:chemistry / Q000494:pharmacology*; D007218:Industrial Microbiology; D008670:Metals / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000378:metabolism*; D013210:Staphylococcus / Q000187:drug effects / Q000502:physiology; D013501:Surface-Active Agents / Q000737:chemistry / Q000494:pharmacology
D000890:Anti-Infective Agents; D006017:Glycolipids; D008670:Metals; D013501:Surface-Active Agents; C418382:rhamnolipid
biofilm inhibition; biofouling; biosurfactants; metal surface; rhamnolipids