A 3D puzzle approach to building protein-DNA structures.
Despite recent advances in structural analysis, it is still challenging to obtain a high-resolution structure for a complex of RNA polymerase, transcriptional factors, and DNA. However, using biochemical constraints, 3D printed models of available structures, and computer modeling, one can build biologically relevant models of such supramolecular complexes.
a Gene Expression and Regulation Section , Laboratory of Cell and Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health , Bethesda , MD , USA.
Deborah M
DM
Hinton
D017122:Bacteriophage T4 / Q000378:metabolism; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D066330:Printing, Three-Dimensional; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism
C078876:AsiA protein, Enterobacteria phage T4; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C067996:MotA protein, Enterobacteria phage T4; D014157:Transcription Factors; D014764:Viral Proteins; D004247:DNA; D012321:DNA-Directed RNA Polymerases
3D printing; AsiA; MotA; RNA polymerase; activation; phage; structure