Effect of porcine glucagon-like peptides-2 on tight junction in GLP-2R + IPEC-J2 cell through the PI3k/Akt/mTOR/p70S6Ksignalling pathway.
Because of rare glucagon-like peptide-2 (GLP-2) receptor (+) cells within the gut mucosa, the molecular mechanisms transducing the diverse actions of GLP-2 remain largely obscure. This research identified the naturally occurring intestinal cell lines that endogenously express GLP-2R and determined the molecular mechanisms of the protective effects of GLP-2-mediated tight junctions (TJ) in GLP-2R (+) cell line. (i) Immunohistochemistry results showed that GLP-2R is localised to the epithelia, laminae propriae and muscle layers of the small and large bowels of newborn piglets. (ii) GLP-2R expression was apparent in the cytoplasm of endocrine cells in IPEC-J2 cell lines. (iii) The protein expressions of ZO-1, claudin-1, occludin, p-PI3K, p-Akt, p-mTOR and p-p70S6Ksignificantly (p < 0.05) increased in GLP-2-treated IPEC-J2 cells, and all of them significantly (p < 0.05) decreased when LY-294002 or rapamycin was added. GLP-2 improves intestinal TJ expression of GLP-2R (+) cells through the PI3k/Akt/mTOR/p70S6Ksignalling pathway.
Institute of Animal Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
K K
KK
Qi
Institute of Animal Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Y Q
YQ
Sun
Institute of Animal Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
J
J
Wan
Institute of Animal Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
B
B
Deng
Institute of Animal Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
X M
XM
Men
Institute of Animal Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
J
J
Wu
Institute of Animal Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Z W
ZW
Xu
D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D053767:Glucagon-Like Peptide 2 / Q000494:pharmacology*; D000067758:Glucagon-Like Peptide-2 Receptor / Q000378:metabolism*; D008297:Male; D058539:Phosphatidylinositol 3-Kinase / Q000235:genetics / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism*; D038762:Ribosomal Protein S6 Kinases, 70-kDa / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013552:Swine; D058570:TOR Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D019108:Tight Junctions / Q000502:physiology
D053767:Glucagon-Like Peptide 2; D000067758:Glucagon-Like Peptide-2 Receptor; D058539:Phosphatidylinositol 3-Kinase; D051057:Proto-Oncogene Proteins c-akt; D038762:Ribosomal Protein S6 Kinases, 70-kDa; D058570:TOR Serine-Threonine Kinases
IPEC-J2; PI3k/Akt/mTOR/p70S6K; porcine glucagon-like peptide-2; tight junction protein