The protective effect of L-arginine, tadalafil, and their combination in rat testes after ischemia and reperfusion injury.
BACKGROUND
Nitric oxide (NO) plays an important role in the ischemia and reperfusion process. In this study, we aimed to examine the effect of L-arginine, tadalafil, and their combination for prevention of the ischemia reperfusion injury after testis torsion in rats.


METHODS
A total of 40 adult, male Sprague-Dawley rats were allocated into five groups. Three hours of left testicular torsion was performed in each group, excluding the control group. While the ischemia reperfusion (I/R) group had no treatment, I/R + Arg group received L-arginine, I/R + Td group received tadalafil and I/R + Arg + Td group received tadalafil and L-arginine 30 minutes before the detorsion. Then the left testis was untwisted for four hours of reperfusion. After bilateral orchiectomy, lipid peroxidation (LPx) and glutathione (GSH) activities were examined in testicular tissue. Spermatogenesis was evaluated with Johnsen's score.


RESULTS
LPx levels of the I/R group were found to be significantly higher than for groups that received drugs for both testes (p<0.001). GSH levels of the combination group were higher than I/R group in ipsilateral testis (p<0.01) and it was significantly higher than other groups for contralateral testis (p<0.001 for I/R group, p<0.01 for I/R + Arg, p<0.05 for I/R + Td). Mean Johnsen's score of the I/R group was found to be significantly lower than treatment groups in ipsilateral testis (p<0.001 for I/R + Arg + Td group, p<0.01 for other treatment goups) and contralateral testis (p<0.001). The mean Johnsen score of the combination group was significantly higher than that of other treatment groups in ipsilateral testis (p<0.05) and it was significantly higher than in the I/R + Td group in the contralateral testis (p<0.05).


CONCLUSIONS
L-arginine, tadalafil, and combination of these two molecules showed protective effect against ischemia/reperfusion injury for both testes after unilateral testis torsion.
Department of Urology; Uludag University, School of Medicine, Bursa, Turkey.
Gokhun
G
Ozmerdiven
Department of Urology; Uludag University, School of Medicine, Bursa, Turkey.
Burhan
B
Coskun
Department of Urology; Uludag University, School of Medicine, Bursa, Turkey.
Onur
O
Kaygisiz
Department of Pathology; Uludag University, School of Medicine, Bursa, Turkey.
Berna Aytac
BA
Vuruskan
Department of Biochemistry; Uludag University, School of Medicine, Bursa, Turkey.
Burak
B
Asiltas
Department of Urology; Uludag University, School of Medicine, Bursa, Turkey.
Hakan
H
Kilicarslan