Deoxynivalenol induces toxic effects in the ovaries of pigs: An ex vivo approach.
Deoxynivalenol (DON) is a frequently found trichothecene mycotoxin that elicits toxic effects on humans and animals. In pigs, DON induces changes in digestive and immune systems. Effects on the reproductive system are scarce and mainly based in in vitro models. The aim of this study was to evaluate, using an ex vivo model, the effects of DON on the morphology of ovaries of pigs in all stages of follicular development. Six 5-month-old pigs were used for sampling the explants. Thirty-six explants were incubated for 48 hours in culture medium (n = 18) or medium containing 10 μM of DON (n = 18). After the incubation period, the explants were submitted to histologic and immunohistochemical (proliferating cell nuclear antigen [PCNA] expression) analysis. Histologic changes were scored, and a lesional score was established. Oocytes and follicular cells immunostained for PCNA were counted. Explants exposed to DON showed a significant increase in the lesional score (P = 0.0004) compared to control explants. The main histologic changes were degeneration of oocytes and granulosa cells, interstitial edema and pyknotic cells. DON induced a reduction in the number of normal follicles in all stages of follicular development: primordial (P = 0.005), primary (P = 0.04), and growing follicles (P = 0.04) compared to control group. Deoxynivalenol also induced a significant increase (P ≤ 0.05) in the number of pyknotic oocytes in all stages of follicular development; however, no significant change in PCNA expression in oocytes or follicular cells was observed. These results indicated that DON induces toxic effects on the ovaries, affecting follicular development and interfering with reproductive parameters on pigs. Also, the present data indicate that ovarian explants are an adequate model for assessing reproductive toxicity.
Laboratory of Animal Pathology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.
Juliana Rubira
JR
Gerez
Laboratory of Animal Pathology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.
Stela Silva
SS
Desto
Laboratory of Animal Pathology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Paraná, Brazil. Electronic address: ana.bracarense@pq.cnpq.br.
Ana Paula Frederico Rodrigues Loureiro
APFRL
Bracarense
D000818:Animals; D005260:Female; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects; D009865:Oocytes / Q000166:cytology / Q000187:drug effects; D006080:Ovarian Follicle / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D010053:Ovary / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D034421:Sus scrofa / Q000502:physiology*; D014255:Trichothecenes / Q000633:toxicity*
D018809:Proliferating Cell Nuclear Antigen; D014255:Trichothecenes; C007262:deoxynivalenol
Follicular development; Mycotoxin; PCNA; Reproductive disorder; Toxicity