Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology.
Rat bone marrow mesenchymal stem cell sheets (rBMSC sheets) are attractive for cell-based tissue engineering. However, methods of culturing rBMSC sheets are critically limited. In order to obtain intact rBMSC sheets, a light-induced cell sheet method was used in this study. TiO2nanodot films were coated with (TL) or without (TN) laminin-521. We investigated the effects of laminin-521 on rBMSCs during cell sheet culturing. The fabricated rBMSC sheets were subsequently assessed to study cell sheet viability, reattachment ability, cell sheet thickness, collagen type I deposition, and multilineage potential. The results showed that laminin-521 could promote the formation of rBMSC sheets with good viability under hyperconfluent conditions. Cell sheet thickness increased from an initial 26.7 ± 1.5μm (day 5) up to 47.7 ± 3.0μm (day 10). Moreover, rBMSC sheets maintained their potential of osteogenic, adipogenic, and chondrogenic differentiation. This study provides a new strategy to obtain rBMSC sheets using light-induced cell sheet technology.
Department of Implantology, Stomatology Hospital, School of Medicine, Zhejiang University, Yan'an Road, Hangzhou, China.
Zhiwei
0000-0001-8203-6151
Z
Jiang
Department of Implantology, Stomatology Hospital, School of Medicine, Zhejiang University, Yan'an Road, Hangzhou, China.
Yue
0000-0002-8753-9097
Y
Xi
Department of Implantology, Stomatology Hospital, School of Medicine, Zhejiang University, Yan'an Road, Hangzhou, China.
Kaichen
0000-0002-3337-8718
K
Lai
Department of Oral Medicine, Stomatology Hospital, School of Medicine, Zhejiang University, Yan'an Road, Hangzhou, China.
Ying
0000-0003-3536-5037
Y
Wang
Department of Oral and Maxillofacial Surgery, Stomatology Hospital, School of Medicine, Zhejiang University, Yan'an Road, Hangzhou, China.
Huiming
0000-0002-3742-1146
H
Wang
Department of Implantology, Stomatology Hospital, School of Medicine, Zhejiang University, Yan'an Road, Hangzhou, China.
Guoli
0000-0002-6000-5378
G
Yang
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000327:Adsorption; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000528:radiation effects; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D007797:Laminin / Q000494:pharmacology*; D008027:Light; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D053758:Nanoparticles / Q000737:chemistry; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D013194:Staining and Labeling
D024042:Collagen Type I; D007797:Laminin; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine