Taraxerol, a pentacyclic triterpene from Abroma augusta leaf, attenuates acute inflammation via inhibition of NF-κB signaling.
Abroma augusta L. (Malvaceae) leaf is traditionally used to treat inflammatory disorders. In our laboratory, we have scientifically validated the anti-inflammatory effect of A. augusta leaf extract. In this study, it has been aimed to evaluate in vivo anti-inflammatory effect of taraxerol isolated from the methanol extract of A. augusta leaf. It was further intended to find out the probable mechanism of anti-inflammatory effect of taraxerol. The anti-inflammatory effect of taraxerol (5 and 10mg/kg, i.p.) was measured employing carrageenan-induced paw edema model of acute inflammation. The carrageenan injection resulted significant edema formation in the right paw when compared with un-injected left paw. However, taraxerol (10mg/kg) treatment could significantly (p<0.05-0.01) attenuate carrageenan induced paw edema 2h onward. The effect of taraxerol at the dose of 5mg/kg was found to be significant (p<0.05) only after 4h of carrageenan treatment. Taraxerol (10mg/kg) treatment could significantly (p<0.01) attenuate carrageenan mediated up-regulation in the levels of IL 1β, IL 6, IL 12 and TNF α in the right paw tissues. In search of molecular mechanism, taraxerol (10mg/kg) could significantly (p<0.05-0.01) reinstate carrageenan provoked NF-κB signaling and thereby caused significant down-regulation in the expressions of COX-2 (p<0.01) and iNOS (p<0.05). In conclusion, taraxerol would attenuate acute inflammation via inhibition of NF-κB signaling.
Advanced Pharmacognosy Research Laboratory, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India.
Ritu
R
Khanra
Advanced Pharmacognosy Research Laboratory, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India. Electronic address: s.dewanjee@yahoo.com.
Saikat
S
Dewanjee
Advanced Pharmacognosy Research Laboratory, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India.
Tarun K
TK
Dua
Advanced Pharmacognosy Research Laboratory, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India.
Niloy
N
Bhattacharjee
D000818:Animals; D002351:Carrageenan; D016207:Cytokines / Q000378:metabolism; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology; D007249:Inflammation / Q000188:drug therapy* / Q000378:metabolism*; D018836:Inflammation Mediators / Q000378:metabolism; D019660:Malvaceae / Q000737:chemistry*; D016328:NF-kappa B / Q000378:metabolism*; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry*; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects; D014315:Triterpenes / Q000494:pharmacology*
D016207:Cytokines; D018836:Inflammation Mediators; D016328:NF-kappa B; D014315:Triterpenes; D009828:Oleanolic Acid; D002351:Carrageenan; C005802:taraxerol
Abroma augusta; Carrageenan; Inflammation; NF-κB; Taraxerol