Preparation of Slice Cultures from Rodent Hippocampus.
This protocol describes the preparation of hippocampal slice cultures from rat or mouse pups using sterile conditions that do not require the use of antibiotics or antimycotics. Combining very good optical and electrophysiological accessibility with a lifetime approaching that of the intact animal, many fundamental questions about synaptic plasticity and long-term dynamics of network connectivity can be addressed with this preparation.
Institute for Synaptic Physiology, Center for Molecular Neurobiology (ZMNH), 20251 Hamburg, Germany.
Christine E
0000-0003-0345-3665
CE
Gee
Institute for Synaptic Physiology, Center for Molecular Neurobiology (ZMNH), 20251 Hamburg, Germany.
Iris
I
Ohmert
Institute for Synaptic Physiology, Center for Molecular Neurobiology (ZMNH), 20251 Hamburg, Germany.
J Simon
0000-0003-0893-9349
JS
Wiegert
Institute for Synaptic Physiology, Center for Molecular Neurobiology (ZMNH), 20251 Hamburg, Germany thomas.oertner@zmnh.uni-hamburg.de.
Thomas G
0000-0002-2312-7528
TG
Oertner
D000818:Animals; D006624:Hippocampus / Q000502:physiology*; D051379:Mice; D009474:Neurons / Q000502:physiology*; D009924:Organ Culture Techniques / Q000379:methods*; D051381:Rats; D013569:Synapses / Q000502:physiology