Collinearity between potato (Solanum tuberosum L.) and wild relatives assessed by comparative cytogenetic mapping.
A major bottleneck to introgressive hybridization is the lack of genome collinearity between the donor (alien) genome and the recipient crop genome. Structural differences between the homeologs may create unbalanced segregation of chromosomes or cause linkage drag. To assess large-scale collinearity between potato and two of its wild relatives (Solanum commersonii and Solanum chacoense), we used BAC-FISH mapping of sequences with known positions on the RH potato map. BAC probes could successfully be hybridized to the S. commersonii and S. chachoense pachytene chromosomes, confirming their correspondence with linkage groups in RH potato. Our study shows that the order of BAC signals is conserved. Distances between BAC signals were quantified and compared; some differences found suggest either small-scale rearrangements or reduction/amplification of repeats. We conclude that S. commersonii and S. chacoense are collinear with cultivated Solanum tuberosum on the whole chromosome scale, making these amenable species for efficient introgressive hybridization breeding.
a Department of Plant Biology, Facultad de Agronomía, Universidad de la República, Garzón 780, PC 12900, Montevideo, Uruguay.|b Laboratory of Genetics, Wageningen University, Droevendaalsesteeg 1, P.O. Box 16, 6708 PB, Wageningen, the Netherlands.
Paola
P
Gaiero
b Laboratory of Genetics, Wageningen University, Droevendaalsesteeg 1, P.O. Box 16, 6708 PB, Wageningen, the Netherlands.
José
J
van de Belt
c Horticulture Unit, National Institute for Agricultural Research, Ruta 48 km 10, Las Brujas, Uruguay.
Francisco
F
Vilaró
d Biosystematics Group, Wageningen University, Wageningen, the Netherlands.
M Eric
ME
Schranz
a Department of Plant Biology, Facultad de Agronomía, Universidad de la República, Garzón 780, PC 12900, Montevideo, Uruguay.
Pablo
P
Speranza
b Laboratory of Genetics, Wageningen University, Droevendaalsesteeg 1, P.O. Box 16, 6708 PB, Wageningen, the Netherlands.
Hans
H
de Jong
D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D032461:Chromosomes, Plant; D018744:DNA, Plant / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D017343:Genes, Plant; D008040:Genetic Linkage; D014644:Genetic Variation; D018745:Genome, Plant; D006824:Hybridization, Genetic; D007091:Image Processing, Computer-Assisted; D017404:In Situ Hybridization, Fluorescence; D008957:Models, Genetic; D000069600:Plant Breeding; D032322:Solanum / Q000235:genetics; D011198:Solanum tuberosum / Q000145:classification / Q000235:genetics*; D013045:Species Specificity
D018744:DNA, Plant; D004275:DNA, Ribosomal
BAC-FISH multicolore; Solanum chacoense; Solanum commersonii; hybridation introgressive; introgressive hybridisation; macrosynteny; macrosynténie; multicolour BAC-FISH; pomme de terre; potato