Tissue Fixation and Processing for the Histological Identification of Lipids.
Lipids are a heterogeneous group of substances characterized by their solubility in organic solvents and insolubility in water. Lipids can be found as normal components of different tissues and organs, and they can be affected by several pathological conditions. The histochemical identification of lipids plays an important role in histopathological diagnosis and research, but successful staining depends on adequate fixation and processing of the tissue. Here we describe methods to fix and process tissue samples for the histochemical identification of lipids in frozen or paraffin-embedded tissues.
Tissue Engineering Group, Department of Histology, Faculty of Medicine, University of Granada, Avenida de la investigación n° 11, Torre A, 5ª planta, 18016, Granada, Spain. vcarriel@ugr.es.|Instituto de Investigación Biosanitaria Ibs, Granada, Spain. vcarriel@ugr.es.
Víctor
V
Carriel
Tissue Engineering Group, Department of Histology, Faculty of Medicine, University of Granada, Avenida de la investigación n° 11, Torre A, 5ª planta, 18016, Granada, Spain.
Fernando
F
Campos
Pathology Unit, Hospital Universitario Virgen de las Nieves, Granada, Spain.
José
J
Aneiros-Fernández
Department of Anatomy and Cell Biology, University of Western Ontario, London, ON, Canada.
John A
JA
Kiernan
D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D008055:Lipids; D016612:Paraffin Embedding / Q000379:methods; D016707:Tissue Fixation / Q000379:methods
D008055:Lipids
Formaldehyde; Freezing techniques; Isopentane; Lecithin; Linoleic acid; Lipid histochemistry; Lipids fixation; Liquid nitrogen; Paraffin embedding; Sucrose cryoprotection