Genotypic analysis of virulence genes and antimicrobial profile of diarrheagenic Escherichia coli isolated from diseased lambs in Iran.
The aim of the present study was to determine the analysis of virulence genes and antimicrobial profile of diarrheagenic Escherichia coli isolated from diseased lambs. Two hundred ninety E. coli isolates were recovered from 300 rectal swabs of diarrheic lambs and were confirmed by biochemical tests. The pathotype determination was done according to the presence of genes including f5, f41, LTI, STI, bfp, ipaH, stx1, stx2, eae, ehlyA, cnf1, cnf2, cdIII, cdIV, and f17 by PCR method. Sixty-six isolates (23.72%) possessed the STI gene and categorized into entrotoxigenic E. coli (ETEC). Nine isolates (3.1%) and five isolates (1.72%) were positive for the cnf1 and cnf2 genes which categorized into necrotoxic E. coli (NTEC). Hundred and seventeen isolates (40.34%) harbored stx1and/or stx2and classified as Shiga toxin-producing E. coli (STEC). Thirteen isolates (4.48%) were assigned to atypical entropathogenic E. coli (aEPEC) and possessed eae gene. Two isolates (0.68%) were positive for ipaH gene and were assigned to entroinvasive E. coli (EIEC). Statistical analysis showed a specific association between eae gene and STEC pathotype (P < 0.0001). The most prevalent resistance was observed against lincomycin (96.5%) and the lowest resistance was against kanamycine (56.02%), respectively. The high prevalence of STEC and ETEC indicates that diarrheic lambs represent an important reservoir for humans. ETEC may play an important role for frequent occurrence of diarrhea in lambs observed in this region. Due to high antibiotic resistance, appropriate control should be implemented in veterinary medicine to curb the development of novel resistant isolates.
Molecular Microbiology Research Group, Shahid Bahonar University of Kerman, Kerman, Iran.
Reza
R
Ghanbarpour
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran. Nasrinaskari@yahoo.com.
Nasrin
http://orcid.org/0000-0002-8677-3789
N
Askari
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Masoud
M
Ghorbanpour
Razi Vaccine and Serum Research Institute, Shiraz, Iran.
Yahya
Y
Tahamtan
South of Kerman Provincial Veterinary Service, Kerman, Iran.
Khoobyar
K
Mashayekhi
South of Kerman Provincial Veterinary Service, Kerman, Iran.
Narjes
N
Afsharipour
South of Kerman Provincial Veterinary Service, Kerman, Iran.
Nasim
N
Darijani
D000818:Animals; D000831:Animals, Newborn; D000890:Anti-Infective Agents / Q000494:pharmacology; D004269:DNA, Bacterial / Q000032:analysis; D003967:Diarrhea / Q000662:veterinary; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D005260:Female; D007492:Iran / Q000453:epidemiology; D008297:Male; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology* / Q000382:microbiology; D037521:Virulence Factors / Q000235:genetics
D000890:Anti-Infective Agents; D004269:DNA, Bacterial; D037521:Virulence Factors
Antibiotic resistance; Diarrheic lambs; Escherichia coli; Virulence genes