Microscopic identification and in vitro activity of Agastache rugosa (Fisch. et Mey) from Xinjiang, China.
BACKGROUND
Agastache rugosa is well-known as a common traditional Chinese medicine, which have relieving summer-heat, analgesic and antipyretic effects, have long been used as folkloristic remedy in the treatment of several infectious diseases, anti-inflammatory, and for its antibacterial properties. Considering the lack of available data on the morphology, anatomy and in vitro activity of A. rugosa, the goal of the present study was to carry out the microscopic identification of its aerial parts and in vitro activity research as a contribution to the quality control and reasonable utilization involving A. rugosa.


METHODS
The present study was (a) to describe the microscopic identification with usual light and scanning electron microtechniques of A. rugosa, collected from Xinjiang Region; (b) based on previous research on the essential oil constituents among different parts of A. rugosa from Xinjiang by GC-MS method, to evaluate its antibacterial effect and cell viabilitity assay.


RESULTS
The microscopic identification of botanical material showed some typical structure. The essential oils from the dried flower (EOF) and leaves (EOL) of A. rugosa were 0.29% and 0.57% (w/w), respectively. The in vitro antibacterial activities showed strong inhibition against S.aureus, E. coli of EOF; strong inhibition against E. coli of EOL. Based GC-MS analysis, the MTT assay showed a dose and time-dependent increase in damage for gastric cancer cell line SGC-7901.


CONCLUSIONS
The results of this work, based on an extensive analytical characterization of the EOF and EOL chemical composition, compared with other origins, showed A. rugosa possessed antibacterial and cytotoxicity properties activities, which need much additional work to open up new biomedical application of these components.
Postdoctoral research station of college of pharmacy, Xinjiang Medical University, Xinshi District, No. 393 Xinyi Road, Urumqi, Xinjiang, People's Republic of China.|The Fifth Affiliated Hospital of Xinjiang Medical University, Xinshi District, No. 118 Henan West Road, Urumqi, Xinjiang, People's Republic of China.
Haiyan
H
Gong
The Third Affiliated Hospital of Xinjiang Medical University, Xinshi District, No. 789 Suzhou East Road, Urumqi, Xinjiang, People's Republic of China.|Xinjiang Medical University, Xinshi District No. 393 Xinyi Road, Urumqi, Xinjiang, People's Republic of China.
Shaoyu
S
Li
Xinjiang Medical University, Xinshi District No. 393 Xinyi Road, Urumqi, Xinjiang, People's Republic of China.
Lijuan
L
He
Xinjiang Medical University, Xinshi District No. 393 Xinyi Road, Urumqi, Xinjiang, People's Republic of China. gonghaiyan1217@sina.com.
Rena
R
Kasimu
D031327:Agastache; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D045744:Cell Line, Tumor / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008516:Medicine, Chinese Traditional; D008826:Microbial Sensitivity Tests; D008517:Phytotherapy; D035261:Plant Components, Aerial / Q000033:anatomy & histology; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects; D013274:Stomach Neoplasms / Q000188:drug therapy
D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D010938:Plant Oils
Agastache rugosa; Antibacterial effect; Cytotoxicity; Microscopic identification