Short stop mediates axonal compartmentalization of mucin-type core 1 glycans.
T antigen, mucin-type core 1 O-glycan, is highly expressed in the embryonic central nervous system (CNS) and co-localizes with a Drosophila CNS marker, BP102 antigen. BP102 antigen and Derailed, an axon guidance receptor, are localized specifically in the proximal axon segment of isolated primary cultured neurons, and their mobility is restricted at the intra-axonal boundary by a diffusion barrier. However, the preferred trafficking mechanism remains unknown. In this study, the major O-glycan T antigen was found to localize within the proximal compartments of primary cultured Drosophila neurons, whereas the N-glycan HRP antigen was not. Ultrastructural analysis by atmospheric scanning electron microscopy revealed that microtubule bundles cross one another at the intra-axonal boundary, and that T antigens form circular pattern before the boundary. We then identified Short stop (Shot), a crosslinker protein between F-actin and microtubules, as a mediator for the proximal localization of T antigens; null mutation of shot cancelled preferential localization of T antigens. Moreover, F-actin binding domain of Shot was required for their proximal localization. Together, our results allow us to propose a novel trafficking pathway where Shot crosslinks F-actin and microtubules around the intra-axonal boundary, directing T antigen-carrying vesicles toward the proximal plasma membrane.
Laboratory of Cell Biology, Department of Bioinformatics, Graduate School of Engineering, Soka University, 1-236 Tangi-machi, Hachioji-shi, Tokyo 192-8577, Japan.
Takaaki
T
Kinoshita
Biomedical Research Institute, National Institute of Industrial Science and Technology (AIST) Tsukuba Central 6 and 2, 1-1-1 Higashi, Tsukuba-shi, Ibaraki 305-8566, Japan.
Chikara
C
Sato
Laboratory of Cell Biology, Department of Bioinformatics, Graduate School of Engineering, Soka University, 1-236 Tangi-machi, Hachioji-shi, Tokyo 192-8577, Japan.
Takashi J
TJ
Fuwa
Laboratory of Cell Biology, Department of Bioinformatics, Graduate School of Engineering, Soka University, 1-236 Tangi-machi, Hachioji-shi, Tokyo 192-8577, Japan.
Shoko
S
Nishihara
D000199:Actins / Q000378:metabolism; D000818:Animals; D000957:Antigens, Viral, Tumor; D001369:Axons / Q000378:metabolism* / Q000648:ultrastructure; D002478:Cells, Cultured; D029721:Drosophila Proteins / Q000737:chemistry / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000378:metabolism*; D008840:Microfilament Proteins / Q000737:chemistry / Q000378:metabolism*; D008954:Models, Biological; D009077:Mucins / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D009474:Neurons / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D011485:Protein Binding; D000072417:Protein Domains; D014404:Tubulin / Q000378:metabolism
D000199:Actins; D000957:Antigens, Viral, Tumor; D029721:Drosophila Proteins; D008840:Microfilament Proteins; D009077:Mucins; D011134:Polysaccharides; D014404:Tubulin; C450828:shot protein, Drosophila