Influence of nonylphenol and octylphenol exposure on 5-HT, 5-HT transporter, and 5-HT2Areceptor.
Nonylphenol (NP) and octylphenol (OP) are priority environmental contaminants that have a potential role as endocrine disruptors. They can be biomagnified in the food chain and pose an estrogenic health risk to human health. A 28-day oral toxicity study was performed to observe the impact of single and combined exposure to NP and OP on 5-HT transporter (SERT) as well as 5-HT2Areceptor. Results showed that the 5-HT levels in rat plasma increased with exposure to middle-dose and high-dose NP, to high-dose OP, and to low, middle, and high doses of combined NP and OP (P < 0.05), while the 5-HT levels in rat platelets increased when exposed to NP/OP or combined NP and OP of middle or high dose (P < 0.05). The expression levels of SERT in rat platelets decreased when exposed to high-dose NP/OP or high dose of combined NP and OP (P < 0.05). Meanwhile, the expression levels of 5-HT2Ain rat platelets decreased when exposed to high-dose NP/OP as well as combined NP and OP (P < 0.05). These findings suggested that exposure to NP and OP could influence the metabolic network of 5-hydroxytryptamine via transportation and receptor binding pathways.
College of Food Science, South China Agricultural University, Guangzhou, 510642, China. liuch@scau.edu.cn.|Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou, 510642, China. liuch@scau.edu.cn.|Laboratory of Quality and Safety Risk Assessment to Post-Harvested Product Storage, Ministry of Agriculture, Guangzhou, 510642, China. liuch@scau.edu.cn.
Chunhong
http://orcid.org/0000-0003-4354-9151
C
Liu
College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
Yuting
Y
Lai
College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
Junyan
J
Ouyang
College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
Tongwang
T
Yang
College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
Youting
Y
Guo
College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
Jie
J
Yang
College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
Shaowen
S
Huang
D000818:Animals; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D004347:Drug Interactions; D052244:Endocrine Disruptors; D008297:Male; D026901:Membrane Transport Proteins; D010636:Phenols / Q000633:toxicity*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D044402:Receptor, Serotonin, 5-HT2A / Q000378:metabolism*; D012701:Serotonin / Q000097:blood*; D050486:Serotonin Plasma Membrane Transport Proteins / Q000378:metabolism*; D018675:Toxicity Tests
D052244:Endocrine Disruptors; D026901:Membrane Transport Proteins; D010636:Phenols; D044402:Receptor, Serotonin, 5-HT2A; D050486:Serotonin Plasma Membrane Transport Proteins; C493232:Slc6a4 protein, rat; C474055:octylphenol; D012701:Serotonin; C025256:nonylphenol
5-HT2A receptor; 5-Hydroxytryptamine; 5-Hydroxytryptamine transporter; Exposure; Nonylphenol; Octylphenol