Levels of salivary thiocyanate and its relation with occurrence of micronuclei using exfoliative cytology in smokers and nonsmokers.
OBJECTIVE
To evaluate the levels of salivary thiocyanate and its relation with the occurrence of micronuclei (MN) using exfoliative cytology in smokers and nonsmokers.


METHODS
One hundred and twenty patients were divided into 3 groups: nonsmoker group 1 (control), smokers group 2, and smokers group 3. Their saliva was collected and analyzed for thiocyanate levels, and exfoliative cytology was evaluated for the presence of MN.


METHODS
Fisher's exact test and ANOVA test were used.


RESULTS
It was seen that as the grade of smoking increased, the levels of salivary thiocyanate and occurrence of MN increased.


CONCLUSIONS
Detection and quantification of "biomarkers" such as salivary thiocyanate and MN in noninvasive and painless procedures such as oral exfoliative cytology can be an upcoming research domain in the field of cancer prevention and therapeutics.
Department of Oral Pathology and Microbiology, M.A. Rangoonwala College of Dental Sciences and Research Center, Pune, Maharashtra, India.
Prachi Shrigopal
PS
Baldawa
Department of Oral Pathology and Microbiology, Bharati Vidyapeeth Dental College and Hospital, Pune, Maharashtra, India.
Venkatesh V
VV
Kulkarni
Department of Oral Pathology and Microbiology, M.A. Rangoonwala Dental College, Pune, Maharashtra, India.
Ajit V
AV
Koshy
Department of Oral Pathology and Microbiology, M.A. Rangoonwala Dental College, Pune, Maharashtra, India.
Sabeer S
SS
Shaikh
Department of Oral Pathology and Microbiology, M.A. Rangoonwala Dental College, Pune, Maharashtra, India.
Rucha
R
Varu
Department of Oral Pathology and Microbiology, M.A. Rangoonwala Dental College, Pune, Maharashtra, India.
Garima
G
Srivastava
D000328:Adult; D015415:Biomarkers / Q000032:analysis; D003581:Cytodiagnosis / Q000379:methods*; D003945:Diagnosis, Oral / Q000379:methods*; D006801:Humans; D008120:Lobeline; D015162:Micronucleus Tests / Q000379:methods; D008875:Middle Aged; D009061:Mouth Mucosa / Q000473:pathology; D009062:Mouth Neoplasms / Q000517:prevention & control; D012463:Saliva / Q000737:chemistry*; D000074285:Smokers; D012907:Smoking / Q000473:pathology; D013861:Thiocyanates / Q000032:analysis*
D015415:Biomarkers; D013861:Thiocyanates; D008120:Lobeline; C031760:thiocyanate