Expression and Function Analysis of MicroRNA-29b in Xuanwei Lung Cancer.
BACKGROUND
In lung cancer, miR-29b can act as a tumor suppressor, influencing epigenetic regulation, cell proliferation, differentiation, apoptosis, metastasis, and chemosensitivity. However, its expression and biological function in Xuanwei lung cancer (XWLC) remains unknown.


METHODS
Fifty pairs of XWLC and matched normal tissues were collected and analyzed for the expression of miR29b by quantitative RT-PCR. MiR-29b precursor and inhibitor were transfected into XWLC-05 cells to investigate its role on regulating cell proliferation by MTT assay and cell apoptosis by caspase 3/7/9 assay in vitro. The target genes of miR-29b were predicted and determined by bioinformatics analysis and luciferase reporter assay.


RESULTS
qRT-PCR results showed that the expression level of miR-29b in XWLC tissues was significantly lower than that in the matched normal tissues, with a proportion of 86%. The low expression of miR-29b was significantly associated with lymphatic metastasis. MiR-29b could suppress cell proliferation and promote apoptosis in XWLC-05 cells. According to the bioinformatics analysis and luciferase reporter assay results, PUMA was a target gene of miR-29b in XWLC.


CONCLUSIONS
Our studies provided evidence for the involvement of miR-29b in XWLC lymphatic metastasis and suggested that miR-29b may serve as a new biomarker for diagnosis of XWLC or therapeutic targets.
Guibo
G
Song
Yinyu
Y
Zhou
Ran
R
Chen
Qing
Q
Li
Bin
B
Shan
Yong
Y
Duan
Yuming
Y
Wang
D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D044127:Epigenesis, Genetic; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction
C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D008156:Luciferases