Selection for a Zinc-Finger Protein Contributes to Seed Oil Increase during Soybean Domestication.
Seed oil is a momentous agronomical trait of soybean (Glycine max) targeted by domestication in breeding. Although multiple oil-related genes have been uncovered, knowledge of the regulatory mechanism of seed oil biosynthesis is currently limited. We demonstrate that the seed-preferred geneGmZF351, encoding a tandem CCCH zinc finger protein, is selected during domestication. Further analysis shows thatGmZF351facilitates oil accumulation by directly activatingWRINKLED1,BIOTIN CARBOXYL CARRIER PROTEIN2,3-KETOACYL-ACYL CARRIER PROTEIN SYNTHASE III,DIACYLGLYCEROL O-ACYLTRANSFERASE1, andOLEOSIN2in transgenic Arabidopsis (Arabidopsis thaliana) seeds. Overexpression ofGmZF351in transgenic soybean also activates lipid biosynthesis genes, thereby accelerating seed oil accumulation. TheZF351haplotype from the cultivated soybean group and the wild soybean (Glycine soja) subgroup III correlates well with high gene expression level, seed oil contents and promoter activity, suggesting that selection ofGmZF351expression leads to increased seed oil content in cultivated soybean. Our study provides novel insights into the regulatory mechanism for seed oil accumulation, and the manipulation ofGmZF351may have great potential in the improvement of oil production in soybean and other related crops.
State Key Laboratory of Plant Genomics (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.) and State Key Laboratory of Molecular Developmental Biology (S.L., G.S.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.|University of the Chinese Academy of Sciences, Beijing 100049, China (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.); and.|Institute of Farming and Cultivation, Heilongjiang Provincial Academy of Agricultural Sciences, Harbin 150086, China (Y.B., W.L., Y.L.).
Qing-Tian
QT
Li
State Key Laboratory of Plant Genomics (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.) and State Key Laboratory of Molecular Developmental Biology (S.L., G.S.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.|University of the Chinese Academy of Sciences, Beijing 100049, China (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.); and.|Institute of Farming and Cultivation, Heilongjiang Provincial Academy of Agricultural Sciences, Harbin 150086, China (Y.B., W.L., Y.L.).
Xiang
X
Lu
State Key Laboratory of Plant Genomics (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.) and State Key Laboratory of Molecular Developmental Biology (S.L., G.S.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.|University of the Chinese Academy of Sciences, Beijing 100049, China (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.); and.|Institute of Farming and Cultivation, Heilongjiang Provincial Academy of Agricultural Sciences, Harbin 150086, China (Y.B., W.L., Y.L.).
Qing-Xin
QX
Song
State Key Laboratory of Plant Genomics (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.) and State Key Laboratory of Molecular Developmental Biology (S.L., G.S.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.|University of the Chinese Academy of Sciences, Beijing 100049, China (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.); and.|Institute of Farming and Cultivation, Heilongjiang Provincial Academy of Agricultural Sciences, Harbin 150086, China (Y.B., W.L., Y.L.).
Hao-Wei
HW
Chen
State Key Laboratory of Plant Genomics (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.) and State Key Laboratory of Molecular Developmental Biology (S.L., G.S.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.|University of the Chinese Academy of Sciences, Beijing 100049, China (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.); and.|Institute of Farming and Cultivation, Heilongjiang Provincial Academy of Agricultural Sciences, Harbin 150086, China (Y.B., W.L., Y.L.).
Wei
W
Wei
State Key Laboratory of Plant Genomics (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.) and State Key Laboratory of Molecular Developmental Biology (S.L., G.S.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.|University of the Chinese Academy of Sciences, Beijing 100049, China (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.); and.|Institute of Farming and Cultivation, Heilongjiang Provincial Academy of Agricultural Sciences, Harbin 150086, China (Y.B., W.L., Y.L.).
Jian-Jun
JJ
Tao
State Key Laboratory of Plant Genomics (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.) and State Key Laboratory of Molecular Developmental Biology (S.L., G.S.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.|University of the Chinese Academy of Sciences, Beijing 100049, China (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.); and.|Institute of Farming and Cultivation, Heilongjiang Provincial Academy of Agricultural Sciences, Harbin 150086, China (Y.B., W.L., Y.L.).
Xiao-Hua
0000-0002-2620-4066
XH
Bian
State Key Laboratory of Plant Genomics (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.) and State Key Laboratory of Molecular Developmental Biology (S.L., G.S.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.|University of the Chinese Academy of Sciences, Beijing 100049, China (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.); and.|Institute of Farming and Cultivation, Heilongjiang Provincial Academy of Agricultural Sciences, Harbin 150086, China (Y.B., W.L., Y.L.).
Ming
0000-0002-6248-8000
M
Shen
State Key Laboratory of Plant Genomics (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.) and State Key Laboratory of Molecular Developmental Biology (S.L., G.S.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.|University of the Chinese Academy of Sciences, Beijing 100049, China (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.); and.|Institute of Farming and Cultivation, Heilongjiang Provincial Academy of Agricultural Sciences, Harbin 150086, China (Y.B., W.L., Y.L.).
Biao
B
Ma
State Key Laboratory of Plant Genomics (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.) and State Key Laboratory of Molecular Developmental Biology (S.L., G.S.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.|University of the Chinese Academy of Sciences, Beijing 100049, China (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.); and.|Institute of Farming and Cultivation, Heilongjiang Provincial Academy of Agricultural Sciences, Harbin 150086, China (Y.B., W.L., Y.L.).
Wan-Ke
0000-0003-3475-2852
WK
Zhang
State Key Laboratory of Plant Genomics (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.) and State Key Laboratory of Molecular Developmental Biology (S.L., G.S.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.|University of the Chinese Academy of Sciences, Beijing 100049, China (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.); and.|Institute of Farming and Cultivation, Heilongjiang Provincial Academy of Agricultural Sciences, Harbin 150086, China (Y.B., W.L., Y.L.).
Ying-Dong
YD
Bi
State Key Laboratory of Plant Genomics (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.) and State Key Laboratory of Molecular Developmental Biology (S.L., G.S.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.|University of the Chinese Academy of Sciences, Beijing 100049, China (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.); and.|Institute of Farming and Cultivation, Heilongjiang Provincial Academy of Agricultural Sciences, Harbin 150086, China (Y.B., W.L., Y.L.).
Wei
W
Li
State Key Laboratory of Plant Genomics (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.) and State Key Laboratory of Molecular Developmental Biology (S.L., G.S.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.|University of the Chinese Academy of Sciences, Beijing 100049, China (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.); and.|Institute of Farming and Cultivation, Heilongjiang Provincial Academy of Agricultural Sciences, Harbin 150086, China (Y.B., W.L., Y.L.).
Yong-Cai
YC
Lai
State Key Laboratory of Plant Genomics (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.) and State Key Laboratory of Molecular Developmental Biology (S.L., G.S.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.|University of the Chinese Academy of Sciences, Beijing 100049, China (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.); and.|Institute of Farming and Cultivation, Heilongjiang Provincial Academy of Agricultural Sciences, Harbin 150086, China (Y.B., W.L., Y.L.).
Sin-Man
0000-0001-9037-904X
SM
Lam
State Key Laboratory of Plant Genomics (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.) and State Key Laboratory of Molecular Developmental Biology (S.L., G.S.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.|University of the Chinese Academy of Sciences, Beijing 100049, China (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.); and.|Institute of Farming and Cultivation, Heilongjiang Provincial Academy of Agricultural Sciences, Harbin 150086, China (Y.B., W.L., Y.L.).
Guang-Hou
GH
Shui
State Key Laboratory of Plant Genomics (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.) and State Key Laboratory of Molecular Developmental Biology (S.L., G.S.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; sychen@genetics.ac.cn jszhang@genetics.ac.cn.|University of the Chinese Academy of Sciences, Beijing 100049, China (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.); and sychen@genetics.ac.cn jszhang@genetics.ac.cn.|Institute of Farming and Cultivation, Heilongjiang Provincial Academy of Agricultural Sciences, Harbin 150086, China (Y.B., W.L., Y.L.) sychen@genetics.ac.cn jszhang@genetics.ac.cn.
Shou-Yi
SY
Chen
State Key Laboratory of Plant Genomics (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.) and State Key Laboratory of Molecular Developmental Biology (S.L., G.S.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; sychen@genetics.ac.cn jszhang@genetics.ac.cn.|University of the Chinese Academy of Sciences, Beijing 100049, China (Q.L., X.L., Q.S., H.C., W.W., J.T., X.B., M.S., B.M., W.Z., S.C., J.Z.); and sychen@genetics.ac.cn jszhang@genetics.ac.cn.|Institute of Farming and Cultivation, Heilongjiang Provincial Academy of Agricultural Sciences, Harbin 150086, China (Y.B., W.L., Y.L.) sychen@genetics.ac.cn jszhang@genetics.ac.cn.
Jin-Song
0000-0003-2165-3468
JS
Zhang
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D000071278:Domestication; D005227:Fatty Acids / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D050356:Lipid Metabolism / Q000235:genetics; D008055:Lipids / Q000096:biosynthesis; D010802:Phylogeny; D010938:Plant Oils / Q000378:metabolism*; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013025:Glycine max / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D014280:Triglycerides / Q000378:metabolism; D016335:Zinc Fingers
D005227:Fatty Acids; D008055:Lipids; D010938:Plant Oils; D010940:Plant Proteins; D014280:Triglycerides