Platelet-derived microparticles and soluble factors differentially regulate human endometrial epithelial cell movement.
We previously proposed that platelets promote re-epithelialization during menstruation. As cell movement is one of the important cell behaviors in the process of tissue remodeling, we examined the effects of platelets on endometrial epithelial cell invasion.



The platelets were isolated from healthy women. Using a human endometrial epithelial cell-derived immortalized cell line, EM-E6/E7/hTERT cells, we examined the effects of platelets and platelet-derived condition media with or without microparticles on the morphological and invasive properties of EM-E6/E7/hTERT cells.



Platelets and microparticle-containing conditioned media inhibited Matrigel invasion by EM-E6/E7/hTERT cells along with an increase in cortical ring formation, whereas microparticle-depleted conditioned media promoted their invasion without any significant changes of cortical ring formation.



These results support our previous proposal and newly suggest the dual roles of platelets: platelet-derived soluble factors that promote cell movement in the distant area, and microparticles that induce re-epithelialization by endometrial epithelial cells in the proximal area.
Department of Gynecology and Obstetrics, Kyoto University Graduate School of Medicine, Kyoto, Japan.
Koh
K
Suginami
Department of Obstetrics and Gynecology, Otsu Red Cross Hospital, Otsu, Japan.
Yukiyasu
Y
Sato
Department of Gynecology and Obstetrics, Kyoto University Graduate School of Medicine, Kyoto, Japan.
Akihito
A
Horie
Department of Gynecology and Obstetrics, Kyoto University Graduate School of Medicine, Kyoto, Japan.|Department of Gynecology and Obstetrics, National Hospital Organization Osaka National Hospital, Osaka, Japan.
Hisanori
H
Matsumoto
Department of Gynecology and Obstetrics, Kyoto University Graduate School of Medicine, Kyoto, Japan.
Hirohiko
H
Tani
Department of Obstetrics and Gynecology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan.
Yasunari
Y
Mizumoto
Department of Obstetrics and Gynecology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan.
Masanori
M
Ono
Department of Obstetrics and Gynecology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan.
Ayumi
A
Matsuoka
Department of Obstetrics and Gynecology, Faculty of Medicine, Shimane University, Izumo, Japan.
Satoru
S
Kyo
Institute for Environmental and Gender-specific Medicine, Juntendo University Graduate School of Medicine, Urayasu, Japan.
Yoshihiko
Y
Araki
Department of Gynecology and Obstetrics, Kyoto University Graduate School of Medicine, Kyoto, Japan.|National Hospital Organization Kyoto Medical Center, Kyoto, Japan.
Ikuo
I
Konishi
Department of Gynecology and Obstetrics, Kyoto University Graduate School of Medicine, Kyoto, Japan.|Department of Obstetrics and Gynecology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan.
Hiroshi
0000-0003-3098-9406
H
Fujiwara
D001792:Blood Platelets / Q000378:metabolism*; D002465:Cell Movement / Q000502:physiology*; D055252:Cell-Derived Microparticles / Q000378:metabolism*; D002478:Cells, Cultured; D004717:Endometrium / Q000166:cytology*; D004847:Epithelial Cells / Q000166:cytology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans
chemokines; endometrial epithelium; invasion; microparticle; platelets; tissue remodeling