Cytokeratin-14 contributes to collective invasion of salivary adenoid cystic carcinoma.
Collective invasion of cells plays a fundamental role in tissue growth, wound healing, immune response and cancer metastasis. This paper aimed to investigate cytokeratin-14 (CK14) expression and analyze its association with collective invasion in the invasive front of salivary adenoid cystic carcinoma (SACC) to uncover the role of collective invasion in SACC. Here, in the clinical data of 121 patients with SACC, the positive expression of CK14 was observed in 35/121(28.93%) of the invasive front of SACC. CK14 expression in the invasive front, local regional recurrence and distant metastasis were independent and significant prognostic factors in SACC patients. Then, we found that in an ex vivo 3D culture assay, CK14 siRNA receded the collective invasion, and in 2D monolayer culture, CK14 overexpression induced a collective SACC cell migration. These data indicated that the presence of characterized CK14+ cells in the invasive front of SACC promoted collective cell invasion of SACC and may be a biomarker of SACC with a worse prognosis.
State Key Laboratory of Oral Diseases West China Hospital of Stomatology, Sichuan University, Chengdu, People's Republic of China.
Xiao-Lei
XL
Gao
State Key Laboratory of Oral Diseases West China Hospital of Stomatology, Sichuan University, Chengdu, People's Republic of China.
Jia-Shun
JS
Wu
State Key Laboratory of Oral Diseases West China Hospital of Stomatology, Sichuan University, Chengdu, People's Republic of China.
Min-Xin
MX
Cao
State Key Laboratory of Oral Diseases West China Hospital of Stomatology, Sichuan University, Chengdu, People's Republic of China.
Shi-Yu
SY
Gao
State Key Laboratory of Oral Diseases West China Hospital of Stomatology, Sichuan University, Chengdu, People's Republic of China.
Xiao
X
Cen
State Key Laboratory of Oral Diseases West China Hospital of Stomatology, Sichuan University, Chengdu, People's Republic of China.
Ya-Ping
YP
Jiang
State Key Laboratory of Oral Diseases West China Hospital of Stomatology, Sichuan University, Chengdu, People's Republic of China.
Sha-Sha
SS
Wang
Key Laboratory of Fermentation Engineering, Ministry of Education, Hubei University of Technology, Wuhan, People's Republic of China.
Ya-Jie
YJ
Tang
State Key Laboratory of Oral Diseases West China Hospital of Stomatology, Sichuan University, Chengdu, People's Republic of China.
Qian-Ming
QM
Chen
State Key Laboratory of Oral Diseases West China Hospital of Stomatology, Sichuan University, Chengdu, People's Republic of China.|Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu, People's Republic of China.
Xin-Hua
XH
Liang
State Key Laboratory of Oral Diseases West China Hospital of Stomatology, Sichuan University, Chengdu, People's Republic of China.|Department of Oral Pathology, West China Hospital of Stomatology, Sichuan University, Chengdu, People's Republic of China.
Yaling
Y
Tang
D003528:Carcinoma, Adenoid Cystic / Q000401:mortality / Q000473:pathology / Q000503:physiopathology*; D005260:Female; D006801:Humans; D053547:Keratin-14 / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000503:physiopathology; D012468:Salivary Gland Neoplasms / Q000401:mortality / Q000473:pathology / Q000503:physiopathology*; D046509:Tissue Culture Techniques
D053547:Keratin-14