Determination of pesticides in fatty matrices using gel permeation clean-up followed by GC-MS/MS and LC-MS/MS analysis: A comparison of low- and high-pressure gel permeation columns.
Two low-pressure columns (Bio-Beads SX-3) and three high-pressure GPC columns were compared for clean-up of a wide range of pesticides in fatty matrices of vegetable or animal origin. The GPC fractions were analyzed by GC-MS/MS and LC-MS/MS without additional clean-up. The performance of the GPC clean-up on the five column types was compared in terms of solvent consumption, lipid removal, pesticide recovery and repeatability. It was found that for fatty matrices, mainly consisting of high molecular weight triglycerides i.e. most vegetable oils and animal fats, good fractionation is obtained for the majority of the pesticides. On the other hand, for fats and oils containing relatively high amounts of low molecular weight triglycerides, i.e. butter fat and palm kernel oil, none of the columns provided sufficient clean-up and cause interferences and system contamination, especially in the case of GC-MS/MS analysis. For the latter case, best results in terms of lipid removal and pesticide recovery were obtained on a set (2×300mmlength) of narrow bore (7.5mm ID) columns packed with 5µm PL Gel material. Column loadability is, however, much lower on that set of columns compared the other evaluated GPC columns, impairing overall method sensitivity.
Research Institute for Chromatography, President Kennedypark 26, B-8500 Kortrijk, Belgium. Electronic address: frank.david@richrom.com.
Frank
F
David
Research Institute for Chromatography, President Kennedypark 26, B-8500 Kortrijk, Belgium.
Christophe
C
Devos
Research Institute for Chromatography, President Kennedypark 26, B-8500 Kortrijk, Belgium.
Emmie
E
Dumont
Nestlé Research Centre, Vers-Chez-les-Blanc, PO Box 44, CH-1000 Lausanne 26, Switzerland.
Zhen
Z
Yang
Research Institute for Chromatography, President Kennedypark 26, B-8500 Kortrijk, Belgium.
Pat
P
Sandra
Nestlé Research Centre, Vers-Chez-les-Blanc, PO Box 44, CH-1000 Lausanne 26, Switzerland. Electronic address: fernando.huertas@rdls.nestle.com.
José Fernando
JF
Huertas-Pérez
D002850:Chromatography, Gel / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D005223:Fats / Q000032:analysis* / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D010575:Pesticides / Q000032:analysis* / Q000302:isolation & purification; D011312:Pressure; D053719:Tandem Mass Spectrometry / Q000379:methods*
D005223:Fats; D010575:Pesticides
Clean-up; Fatty matrices; GC-MS/MS; Gel permeation chromatography; LC-MS/MS; Pesticides