A novel human artery model to assess the magnetic accumulation of SPIONs under flow conditions.
Magnetic targeting utilises the properties of superparamagnetic iron oxide nanoparticles (SPIONs) to accumulate particles in specified vasculature regions under an external magnetic field. As the behaviour of circulating particles varies depending on nanoparticle characteristics, magnetic field strength and flow dynamics, we established an improved ex vivo model in order to estimate the magnetic capture of SPIONs in physiological-like settings. We describe here a new, easy to handle ex vivo model of human umbilical artery. Using this model, the magnetic targeting of different types of SPIONs under various external magnetic field gradients and flow conditions was investigated by atomic emission spectroscopy and histology. Among tested particles, SPION-1 with lauric acid shell had the largest capacity to accumulate at the specific artery segment. SPION-2 (lauric acid/albumin-coated) were also successfully targeted, although the observed peak in the iron content under the tip of the magnet was smaller than for SPION-1. In contrast, we did not achieve magnetic accumulation of dextran-coated SPION-3. Taken together, the umbilical artery model constitutes a time- and cost-efficient, 3R-compliant tool to assess magnetic targeting of SPIONs under flow. Our results further imply the possibility of an efficient in vivo targeting of certain types of SPIONs to superficial arteries.
Section of Experimental Oncology und Nanomedicine (SEON), ENT-Department, University Hospital Erlangen, Erlangen, Germany.
Agata
A
Janikowska
Section of Experimental Oncology und Nanomedicine (SEON), ENT-Department, University Hospital Erlangen, Erlangen, Germany.
Jasmin
J
Matuszak
Section of Experimental Oncology und Nanomedicine (SEON), ENT-Department, University Hospital Erlangen, Erlangen, Germany.
Stefan
S
Lyer
Section of Experimental Oncology und Nanomedicine (SEON), ENT-Department, University Hospital Erlangen, Erlangen, Germany.
Eveline
E
Schreiber
Section of Experimental Oncology und Nanomedicine (SEON), ENT-Department, University Hospital Erlangen, Erlangen, Germany.
Harald
H
Unterweger
Section of Experimental Oncology und Nanomedicine (SEON), ENT-Department, University Hospital Erlangen, Erlangen, Germany.
Jan
J
Zaloga
Department of Functional Materials in Medicine and Dentistry, University Hospital Würzburg, Würzburg, Germany.
Jürgen
J
Groll
Section of Experimental Oncology und Nanomedicine (SEON), ENT-Department, University Hospital Erlangen, Erlangen, Germany.
Christoph
C
Alexiou
Section of Experimental Oncology und Nanomedicine (SEON), ENT-Department, University Hospital Erlangen, Erlangen, Germany.
Iwona
I
Cicha
D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008954:Models, Biological; D012212:Rheology; D013997:Time Factors; D014469:Umbilical Arteries / Q000502:physiology*
D058185:Magnetite Nanoparticles