Development of an injectable pseudo-bone thermo-gel for application in small bone fractures.
A pseudo-bone thermo-gel was synthesized and evaluated for its physicochemical, mechanical and rheological properties, with its application to treat small bone fractures. The pseudo-bone thermo-gel was proven to have thermo-responsive properties, behaving as a solution in temperatures below 25°C, and forming a gelling technology when maintained at physiological conditions. Poly propylene fumerate (PPF), Pluronic F127 and PEG-PCL-PEG were strategically blended, obtaining a thermo-responsive delivery system, to mimic the mechanical properties of bone with sufficient matrix hardness and resilience. A Biopharmaceutics Classification System (BCS) class II drug, simvastatin, was loaded in the pseudo-bone thermo-gel, selected for its bone healing properties. In vitro release analysis was undertaken on a series of experimental formulations, with the ideal formulations obtaining its maximum controlled drug release profile up to 14days. Ex vivo studies were undertaken on an induced 4mm diameter butterfly-fractured osteoporotic human clavicle bone samples. X-ray, ultrasound as well as textural analysis, undertaken on the fractured bones before and after treatment displayed significant bone filling, matrix hardening and matrix resilience properties. These characteristics of the pseudo-bone thermo-gel thus proved significant potential for application in small bone fractures.
Wits Advanced Drug Delivery Platform Research Unit, Department of Pharmacy and Pharmacology, School of Therapeutic Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, 7 York Road, Parktown, 2193, South Africa.
Pariksha J
PJ
Kondiah
Wits Advanced Drug Delivery Platform Research Unit, Department of Pharmacy and Pharmacology, School of Therapeutic Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, 7 York Road, Parktown, 2193, South Africa.
Yahya E
YE
Choonara
Wits Advanced Drug Delivery Platform Research Unit, Department of Pharmacy and Pharmacology, School of Therapeutic Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, 7 York Road, Parktown, 2193, South Africa.
Pierre P D
PPD
Kondiah
Wits Advanced Drug Delivery Platform Research Unit, Department of Pharmacy and Pharmacology, School of Therapeutic Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, 7 York Road, Parktown, 2193, South Africa.
Pradeep
P
Kumar
Wits Advanced Drug Delivery Platform Research Unit, Department of Pharmacy and Pharmacology, School of Therapeutic Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, 7 York Road, Parktown, 2193, South Africa.
Thashree
T
Marimuthu
Wits Advanced Drug Delivery Platform Research Unit, Department of Pharmacy and Pharmacology, School of Therapeutic Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, 7 York Road, Parktown, 2193, South Africa.
Lisa C
LC
du Toit
Wits Advanced Drug Delivery Platform Research Unit, Department of Pharmacy and Pharmacology, School of Therapeutic Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, 7 York Road, Parktown, 2193, South Africa. Electronic address: viness.pillay@wits.ac.za.
Viness
V
Pillay
D002968:Clavicle / Q000000981:diagnostic imaging / Q000187:drug effects; D016503:Drug Delivery Systems; D065546:Drug Liberation; D017102:Fracture Healing / Q000187:drug effects; D050723:Fractures, Bone / Q000000981:diagnostic imaging / Q000188:drug therapy*; D005782:Gels / Q000737:chemistry / Q000627:therapeutic use*; D006801:Humans; D020442:Poloxamer / Q000737:chemistry; D011091:Polyesters; D011092:Polyethylene Glycols; D011126:Polypropylenes / Q000737:chemistry; D019821:Simvastatin / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use; D013696:Temperature
D005782:Gels; D011091:Polyesters; D011126:Polypropylenes; C502908:poly(ethylene glycol)-poly(caprolactone)-poly(ethylene glycol); D020442:Poloxamer; D011092:Polyethylene Glycols; D019821:Simvastatin
Bone fractures; Controlled drug release; Pseudo-bone thermo-gel; Thermo-responsive