Vasodilatory effects and underlying mechanisms of the ethyl acetate extracts from Gastrodia elata.
The objective of this study was to assess the ethyl acetate extracts of Gastrodia elata Blume (GEB) on vascular tone and the mechanisms involved. GEB was extracted with 95% EtOH followed by a further extraction with ethyl acetate. The effects of GEB and its ingredients on the isometric tensions of the aortic rings from rats were measured. The ethyl acetate extract of GEB induced a vasodilatory effect on rat aorta, which was partially dependent on endothelium. Four chemical compounds isolated from GEB were identified as 3,4-dihydroxybenzaldehyde (DB), 4-hydroxybenzaldehyde (HB), 4-methoxybenzyl alcohol (MA), and 4,4'-dihydroxydiphenyl methane (DM), respectively. All of these compounds induced vasodilatations, which were dependent on the endothelium to different degrees. After pretreatment with Nω-nitro-l-arginine methyl ester, indomethacin, or methylene blue, the vasodilatations induced by DB, HB, and MA were significantly decreased. In addition, the contractions of the rat aortic rings due to Ca2+influx and intracellular Ca2+release were also inhibited by DM. Furthermore, the administration of DB significantly enhanced the productions of nitric oxide (NO) and the activities of the endothelial NO synthase in aorta and in endothelial cells. Thus, GEB may play an important role in the amelioration of hypertension by modulating vascular tones.
a Department of Pharmacology, Yunnan University of Traditional Chinese Medicine, Kunming 650500, P.R. China.
Rong
R
Dai
b Ethnic Drug Screening & Pharmacology Center, Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission & Ministry of Education, Yunnan Minzu University, Kunming 650500, P.R. China.
Ting
T
Wang
a Department of Pharmacology, Yunnan University of Traditional Chinese Medicine, Kunming 650500, P.R. China.
Xiaoqin
X
Si
a Department of Pharmacology, Yunnan University of Traditional Chinese Medicine, Kunming 650500, P.R. China.
Yuanyuan
Y
Jia
a Department of Pharmacology, Yunnan University of Traditional Chinese Medicine, Kunming 650500, P.R. China.
Lili
L
Wang
b Ethnic Drug Screening & Pharmacology Center, Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission & Ministry of Education, Yunnan Minzu University, Kunming 650500, P.R. China.
Yan
Y
Yuan
a Department of Pharmacology, Yunnan University of Traditional Chinese Medicine, Kunming 650500, P.R. China.
Qing
Q
Lin
b Ethnic Drug Screening & Pharmacology Center, Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission & Ministry of Education, Yunnan Minzu University, Kunming 650500, P.R. China.
Cui
C
Yang
D000085:Acetates / Q000737:chemistry*; D000818:Animals; D001011:Aorta / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D004730:Endothelium, Vascular / Q000187:drug effects; D031668:Gastrodia / Q000737:chemistry*; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014664:Vasodilation / Q000187:drug effects*
D000085:Acetates; D010936:Plant Extracts; C007650:ethyl acetate
Gastrodia elata; cellule endothéliale; endothelial cell; hypertension; vasodilatation