Fast and reliable method for As speciation in urine samples containing low levels of As by LC-ICP-MS: Focus on epidemiological studies.
The speciation analysis of As in urine samples can provide important information for epidemiological studies. Considering that these studies involve hundreds or thousands of samples, a fast and reliable method using a simple LC system with short-length mixed bed ion exchange chromatographic column coupled to ICP-MS for As speciation in human urine samples was developed in this work. Separation of AB+TMAO, DMA, AC, MMA and AsIII+AsVwas accomplished within 5min with good resolution when ammonium carbonate solutions were used as mobile phases and H2O2was added to samples to quantitatively convert AsIII-AsV. Repeatability studies yielded RSD values from 2.0% to 4.8% for all species evaluated. Limits of detection (LOD) for As species ranged from 0.003 to 0.051ngg-1. Application of the method to human urine samples from a non-contaminated area showed that the sum of species measured corresponded to 62-125% of the total As in the sample. The recovery values for these species in urine SRM 2669 were in the range of 89-112% and demonstrated the suitability of the proposed method for epidemiological studies.
University of Missouri Research Reactor, University of Missouri - Columbia, 1513 Research Park Drive, Columbia, MO 65211, United States.
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Carioni
University of Missouri Research Reactor, University of Missouri - Columbia, 1513 Research Park Drive, Columbia, MO 65211, United States; University of Missouri - Columbia, Family and Community Medicine, University of Missouri, 1 Hospital Dr, Columbia MO 65212, United States.
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McElroy
University of Missouri Research Reactor, University of Missouri - Columbia, 1513 Research Park Drive, Columbia, MO 65211, United States.
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Guthrie
University of Missouri Research Reactor, University of Missouri - Columbia, 1513 Research Park Drive, Columbia, MO 65211, United States.
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Ngwenyama
University of Missouri Research Reactor, University of Missouri - Columbia, 1513 Research Park Drive, Columbia, MO 65211, United States. Electronic address: brockmanjd@missouri.edu.
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Brockman
D001152:Arsenicals / Q000737:chemistry* / Q000652:urine*; D002853:Chromatography, Liquid / Q000379:methods*; D016021:Epidemiologic Studies; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D057230:Limit of Detection; D053719:Tandem Mass Spectrometry / Q000379:methods*
D001152:Arsenicals; D006861:Hydrogen Peroxide
Arsenic; Epidemiology; LC-ICP-MS; Low pressure column; Speciation; Urine