Resistant Effect of HLA-DRB1*09 on Hepatitis C Virus Infection Identified by a New Cross-Validation Method.
BACKGROUND
The association of human leukocyte antigen (HLA) with HCV persistence or clearance has not been clarified. We aim to investigate the associations of HLA with HCV infection based on a new cross-validation method.


METHODS
DNA samples from HCV infected patients were collected at random. Sequence based typing (PCR-SBT) was used to detect HLA-A, -B, -DRB1 alleles. The albumin (ALB) levels were detected and compared between the patients carrying the gene and the patients not carrying the gene. The association between the HLA loci and disease status was further confirmed by cross-validation of the two sets of analytical results.


RESULTS
HLA-B*58 and HLA-DRB1*09 showed significant differences in ALB levels between patients with and without the allele (p < 0.05). Only the p-value of the cross-validation for HLA-DRB1*09 was less than 0.05.


CONCLUSIONS
HLA-DRB1*09 has a resistant effect on HCV infection, which is potentially helpful to predict the status of HCV infected patients.
Zhen
Z
Wang
Dong
D
Yang
Meng
M
Cai
Hui
H
Liu
D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D015234:HLA-A Antigens / Q000097:blood / Q000235:genetics*; D015235:HLA-B Antigens / Q000097:blood / Q000235:genetics*; D059811:HLA-DRB1 Chains / Q000097:blood / Q000235:genetics*; D016174:Hepacivirus / Q000276:immunology*; D006526:Hepatitis C / Q000097:blood / Q000276:immunology*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D012709:Serum Albumin / Q000032:analysis*
D015234:HLA-A Antigens; D015235:HLA-B Antigens; D059811:HLA-DRB1 Chains; D012709:Serum Albumin