New Insights on Signal Propagation by Sensory Rhodopsin II/Transducer Complex.
The complex of two membrane proteins, sensory rhodopsin II (NpSRII) with its cognate transducer (NpHtrII), mediates negative phototaxis in halobacteria N. pharaonis. Upon light activation NpSRII triggers a signal transduction chain homologous to the two-component system in eubacterial chemotaxis. Here we report on crystal structures of the ground and active M-state of the complex in the space group I212121. We demonstrate that the relative orientation of symmetrical parts of the dimer is parallel ("U"-shaped) contrary to the gusset-like ("V"-shaped) form of the previously reported structures of the NpSRII/NpHtrII complex in the space group P21212, although the structures of the monomers taken individually are nearly the same. Computer modeling of the HAMP domain in the obtained "V"- and "U"-shaped structures revealed that only the "U"-shaped conformation allows for tight interactions of the receptor with the HAMP domain. This is in line with existing data and supports biological relevance of the "U" shape in the ground state. We suggest that the "V"-shaped structure may correspond to the active state of the complex and transition from the "U" to the "V"-shape of the receptor-transducer complex can be involved in signal transduction from the receptor to the signaling domain of NpHtrII.
Institute of Complex Systems (ICS), ICS-6: Structural Biochemistry, Research Centre Jülich, 52425 Jülich, Germany.|Institute of Crystallography, University of Aachen (RWTH), Jägerstraße 17-19, 52056 Aachen, Germany.
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Ishchenko
Institute of Complex Systems (ICS), ICS-6: Structural Biochemistry, Research Centre Jülich, 52425 Jülich, Germany.|Institut de Biologie Structurale J.-P. Ebel, Université Grenoble Alpes-CEA-CNRS, F-38000 Grenoble, France.
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Round
Institute of Complex Systems (ICS), ICS-6: Structural Biochemistry, Research Centre Jülich, 52425 Jülich, Germany.|Moscow Institute of Physics and Technology, 141700 Dolgoprudniy, Russia.
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Borshchevskiy
CNRS, Laboratoire Jean Kuntzmann, BP 53, Grenoble Cedex 9, France.|NANO-D, INRIA Grenoble-Rhone-Alpes Research Center, 38334 Saint Ismier Cedex, Montbonnot, France.
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Grudinin
Institute of Complex Systems (ICS), ICS-6: Structural Biochemistry, Research Centre Jülich, 52425 Jülich, Germany.|Institut de Biologie Structurale J.-P. Ebel, Université Grenoble Alpes-CEA-CNRS, F-38000 Grenoble, France.|Moscow Institute of Physics and Technology, 141700 Dolgoprudniy, Russia.
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Gushchin
Max-Planck Institute of Molecular Physiology, 44227 Dortmund, Germany.|Department of Physics, University of Osnabrück, Barbarastrasse 7, D-49069 Osnabrück, Germany.
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Klare
Institute of Complex Systems (ICS), ICS-6: Structural Biochemistry, Research Centre Jülich, 52425 Jülich, Germany.
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Remeeva
Institute of Complex Systems (ICS), ICS-6: Structural Biochemistry, Research Centre Jülich, 52425 Jülich, Germany.|Institut de Biologie Structurale J.-P. Ebel, Université Grenoble Alpes-CEA-CNRS, F-38000 Grenoble, France.
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Polovinkin
Moscow Institute of Physics and Technology, 141700 Dolgoprudniy, Russia.
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Utrobin
Institute of Complex Systems (ICS), ICS-6: Structural Biochemistry, Research Centre Jülich, 52425 Jülich, Germany.
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Balandin
Max-Planck Institute of Molecular Physiology, 44227 Dortmund, Germany.
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Engelhard
Institute of Complex Systems (ICS), ICS-6: Structural Biochemistry, Research Centre Jülich, 52425 Jülich, Germany.|Moscow Institute of Physics and Technology, 141700 Dolgoprudniy, Russia.
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Büldt
Institute of Complex Systems (ICS), ICS-6: Structural Biochemistry, Research Centre Jülich, 52425 Jülich, Germany.|Institute of Crystallography, University of Aachen (RWTH), Jägerstraße 17-19, 52056 Aachen, Germany.|Institut de Biologie Structurale J.-P. Ebel, Université Grenoble Alpes-CEA-CNRS, F-38000 Grenoble, France.|Moscow Institute of Physics and Technology, 141700 Dolgoprudniy, Russia.
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Gordeliy
D019843:Archaeal Proteins / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D006216:Halobacteriaceae / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D054730:Protein Interaction Domains and Motifs; D055503:Protein Multimerization; D025603:Sensory Rhodopsins / Q000737:chemistry / Q000378:metabolism*; D015398:Signal Transduction; D055672:Static Electricity; D013329:Structure-Activity Relationship
D019843:Archaeal Proteins; D025603:Sensory Rhodopsins