Candida krusei and Candida glabrata reduce the filamentation of Candida albicans by downregulating expression of HWP1 gene.
Pathogenicity of Candida albicans is associated with its capacity switch from yeast-like to hyphal growth. The hyphal form is capable to penetrate the epithelial surfaces and to damage the host tissues. Therefore, many investigations have focused on mechanisms that control the morphological transitions of C. albicans. Recently, certain studies have showed that non-albicans Candida species can reduce the capacity of C. albicans to form biofilms and to develop candidiasis in animal models. Then, the objective of this study was to evaluate the effects of Candida krusei and Candida glabrata on the morphogenesis of C. albicans. Firstly, the capacity of reference and clinical strains of C. albicans in forming hyphae was tested in vitro. After that, the expression of HWP1 (hyphal wall protein 1) gene was determined by quantitative real-time PCR (polymerase chain reaction) assay. For both reference and clinical strains, a significant inhibition of the hyphae formation was observed when C. albicans was incubated in the presence of C. krusei or C. glabrata compared to the control group composed only by C. albicans. In addition, the culture mixed of C. albicans-C. krusei or C. albicans-C. glabrata reduced significantly the expression of HWP1 gene of C. albicans in relation to single cultures of this specie. In both filamentation and gene expression assays, C. krusei showed the higher inhibitory activity on the morphogenesis of C. albicans compared to C. glabrata. C. krusei and C. glabrata are capable to reduce the filamentation of C. albicans and consequently decrease the expression of the HWP1 gene.
Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, Univ Estadual Paulista - UNESP, Francisco José Longo 777, São Dimas, São José dos Campos, SP, CEP:12245-000, Brazil.
Patrícia Pimentel
PP
de Barros
Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, Univ Estadual Paulista - UNESP, Francisco José Longo 777, São Dimas, São José dos Campos, SP, CEP:12245-000, Brazil.
Fernanda
F
Freire
Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, Univ Estadual Paulista - UNESP, Francisco José Longo 777, São Dimas, São José dos Campos, SP, CEP:12245-000, Brazil. dennisrossoni@hotmail.com.
Rodnei Dennis
http://orcid.org/0000-0002-9977-3040
RD
Rossoni
Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, Univ Estadual Paulista - UNESP, Francisco José Longo 777, São Dimas, São José dos Campos, SP, CEP:12245-000, Brazil.
Juliana Campos
JC
Junqueira
Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, Univ Estadual Paulista - UNESP, Francisco José Longo 777, São Dimas, São José dos Campos, SP, CEP:12245-000, Brazil.
Antonio Olavo Cardoso
AOC
Jorge
D002175:Candida / Q000502:physiology*; D002176:Candida albicans / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D041221:Candida glabrata / Q000502:physiology*; D015536:Down-Regulation; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D025301:Hyphae / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism
D005656:Fungal Proteins; C118102:HWP1 protein, Candida albicans; D008562:Membrane Glycoproteins