Expression of advanced glycation end-products and NFκB in chick embryos exposed to dioxins and treated with acetylsalicylic acid and α-tocopherol.
Dioxins have adverse and multifaceted effect on body functions. They are known to be carcinogens, immunotoxins, and teratogenic agents. In vivo, transformation of dioxins occurs after their interaction with the aryl hydrocarbon receptor (AhR) and leads to formation of proinflammatory and toxic metabolites. The aim of this study was to verify whether α-tocopherol (vitamin E) and acetylsalicylic acid (ASA), could reduce the damage caused by the action of dioxins. Fertile chicken eggs were injected with a solution of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), followed by the injection of α-tocopherol or acetylsalicylic acid. Organs such as heart and liver were dissected from the chick embryos at d 13 and 19 of development and subjected to immunohistochemical analysis of presence of advanced glycation end products (AGEs) and nuclear factor kappa B (NFκB) in tissues. The AGEs were used as the marker for exposure to dioxins, since it is well established that their level increases in dioxin-damaged tissues. Formation of AGEs was evaluated in embryos exposed to dioxin and treated with vitamin E and/or ASA (against dioxin-exposed, untreated controls). We have found that TCDD causes developmental disorders and increases the level of AGEs in chick embryo tissues. The use of such pharmacological agents as vitamin E, ASA, and combination of ASA and vitamin E, inhibited formation of the AGEs in 13-day-old embryos and reduced the AGEs level in embryos after 19 d of the development.
Independent Laboratory of Neurotoxicology and Environmental Diagnostics, Wroclaw Medical University, Wroclaw, Poland.
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Ostrowska
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
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Gostomska-Pampuch
Independent Laboratory of Neurotoxicology and Environmental Diagnostics, Wroclaw Medical University, Wroclaw, Poland.
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Lesków
Department of Animal Physiology and Biostructure, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland.
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Kuropka
Department of Pathomorphology, Wroclaw Medical University, Wroclaw, Poland.
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Gamian
Department of Pathomorphology, Wroclaw Medical University, Wroclaw, Poland.
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Ziólkowski
Institute of Animal Breeding, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland.
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Kowalczyk
Institute of Animal Breeding, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland.
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Lukaszewicz
Department of Medical Biochemistry, Wroclaw Medical University, Wroclaw, Poland.|Wroclaw Research Centre EIT+, Wroclaw, Poland.
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Gamian
Independent Laboratory of Neurotoxicology and Environmental Diagnostics, Wroclaw Medical University, Wroclaw, Poland.
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Calkosinski
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000494:pharmacology; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology; D001241:Aspirin / Q000008:administration & dosage / Q000494:pharmacology*; D002642:Chick Embryo; D017127:Glycation End Products, Advanced / Q000187:drug effects* / Q000378:metabolism; D006321:Heart / Q000187:drug effects / Q000196:embryology; D008099:Liver / Q000187:drug effects / Q000196:embryology; D016328:NF-kappa B / Q000187:drug effects* / Q000378:metabolism; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D013723:Teratogens / Q000633:toxicity; D024502:alpha-Tocopherol / Q000008:administration & dosage / Q000494:pharmacology*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D017127:Glycation End Products, Advanced; D016328:NF-kappa B; D000072317:Polychlorinated Dibenzodioxins; D013723:Teratogens; D024502:alpha-Tocopherol; D001241:Aspirin
TCDD; acetylsalicylic acid; advanced glycation end-product; chick embryo; α-tocopherol