Characterizing Satellite Cells and Myogenic Progenitors During Skeletal Muscle Regeneration.
Muscle stem cells, named satellite cells, are quiescent in resting skeletal muscle. Following injury, satellite cells are activated and become proliferating myoblasts that either self-renew or differentiate. Several markers are used to identify the different myogenic populations, such as Pax7 (quiescent and activated satellite cells), MyoD (proliferating myoblasts), and myogenin (differentiating myoblasts). Immunodetection of these markers is a very useful tool to analyze myogenic cells and muscle regeneration. Here, we describe a method for immunodetection of satellite cells and their myogenic progeny in resting and regenerating skeletal muscles.
Centre for Stem Cell Research, Ottawa Hospital Research Institute, 501 Smyth Road, Ottawa, ON, Canada, K1H 8L6.|Faculty of Medicine, Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.|Sainte-Justine Hospital Research Center, and Department of Rehabilitation, Faculty of Medicine, University of Montreal, Montreal, QC, Canada.
Nicolas A
NA
Dumont
Centre for Stem Cell Research, Ottawa Hospital Research Institute, 501 Smyth Road, Ottawa, ON, Canada, K1H 8L6. mrudnicki@ohri.ca.|Faculty of Medicine, Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada. mrudnicki@ohri.ca.
Michael A
MA
Rudnicki
D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D024510:Muscle Development / Q000502:physiology; D018482:Muscle, Skeletal / Q000378:metabolism*; D012038:Regeneration; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology* / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*
D000943:Antigens, Differentiation
Differentiation markers; Immunochemistry; Immunofluorescence; Myoblasts; Myogenesis; Myogenin; Pax7; Regeneration; Satellite cells; Skeletal muscle