Aerobic-heterotrophic nitrogen removal through nitrate reduction and ammonium assimilation by marine bacterium Vibrio sp. Y1-5.
An aerobic marine bacterium Vibrio sp. Y1-5 was screened to achieve efficient nitrate and ammonium removal simultaneously and fix nitrogen in cells without N loss. Approximately 98.0% of nitrate (100mg/L) was removed in 48h through assimilatory nitrate reduction and nitrate reductase was detected in the cytoplasm. Instead of nitrification, the strain assimilated ammonium directly, and it could tolerate as high as 1600mg/L ammonium concentration while removing 844.6mg/L. In addition, ammonium assimilation occurred preferentially in the medium containing nitrate and ammonium with a total nitrogen (TN) removal efficiency of 80.4%. The results of nitrogen balance and Fourier infrared spectra illustrated that the removed nitrogen was all transformed to protein or stored as organic nitrogen substances in cells and no N was lost in the process. Toxicological studies with the brine shrimp species Artemia naupliia indicated that Vibrio sp. Y1-5 can be applied in aquatic ecosystems safely.
School of Environmental Science and Engineering, Shandong University, Jinan, Shandong 250100, China.
Yating
Y
Li
School of Environmental Science and Engineering, Shandong University, Jinan, Shandong 250100, China.
Yanru
Y
Wang
School of Environmental Science and Engineering, Shandong University, Jinan, Shandong 250100, China.
Lin
L
Fu
School of Environmental Science and Engineering, Shandong University, Jinan, Shandong 250100, China.
Yizhan
Y
Gao
School of Environmental Science and Engineering, Shandong University, Jinan, Shandong 250100, China.
Haixia
H
Zhao
School of Environmental Science and Engineering, Shandong University, Jinan, Shandong 250100, China. Electronic address: wzzhou@sdu.edu.cn.
Weizhi
W
Zhou
D000332:Aerobiosis / Q000187:drug effects; D064751:Ammonium Compounds / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D059001:Aquatic Organisms / Q000187:drug effects / Q000378:metabolism*; D001156:Artemia / Q000502:physiology; D001673:Biodegradation, Environmental / Q000187:drug effects; D003470:Culture Media; D017753:Ecosystem; D052836:Heterotrophic Processes / Q000187:drug effects; D009243:NAD / Q000378:metabolism; D050901:Nitrate Reductase / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism; D009584:Nitrogen / Q000302:isolation & purification*; D010084:Oxidation-Reduction / Q000187:drug effects; D010802:Phylogeny; D017550:Spectroscopy, Fourier Transform Infrared; D014733:Vibrio / Q000378:metabolism*
D064751:Ammonium Compounds; D003470:Culture Media; D009566:Nitrates; D009573:Nitrites; D009243:NAD; D050901:Nitrate Reductase; D009584:Nitrogen
Assimilatory nitrate reduction; N-fixation; Nitrogen removal; Vibrio sp. Y1-5