Declined Natural Killer Cells Emerging in Women with Unexplained Recurrent Spontaneous Abortion and Further Reducing after Medical Therapy.
BACKGROUND
As focus grows on reproduction, the issue of Recurrent Spontaneous Abortion (RSA), especially for unexplained reasons (URSA), is grabbing more and more attention in gynecological immunology. We investigated the changes of peripheral lymphocyte subsets focusing on whether they had some relationship with development of URSA.


METHODS
The percentage and absolute count of lymphocyte subsets (T cells, Th cells, Ts cells, B cells, NK cells) were simultaneously evaluated by flow cytometry in URSA patients (n = 48) and healthy controls (HC, n = 22).


RESULTS
Significantly lower percentage and absolute counts of NKT cells and NK cells were observed in URSA compared to the HC. After medical therapy, an obviously increase was shown in the percentage of both T cells and B cells, whereas it presented a reduction in the percentage of NK cells.


CONCLUSIONS
The flow cytometry test in T, B, NK cells is a method available to identify URSA patients from healthy women and to provide reference guides for clinical therapy.
Xianghua
X
Lin
Dijin
D
Lin
Jiao
J
Liu
Yunwen
Y
Bao
Zhaofan
Z
Luo
Junyue
J
Fang
Ying
Y
Wang
Mingpeng
M
Zhao
Chaohui
C
Duan
D000026:Abortion, Habitual / Q000097:blood / Q000175:diagnosis / Q000276:immunology* / Q000628:therapy; D000328:Adult; D016175:B-Lymphocyte Subsets / Q000276:immunology; D016022:Case-Control Studies; D015536:Down-Regulation; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D018655:Lymphocyte Count; D011237:Predictive Value of Tests; D011247:Pregnancy; D011379:Prognosis; D016176:T-Lymphocyte Subsets / Q000276:immunology; D055815:Young Adult