Beta-catenin participates in dialysate-induced peritoneal fibrosisviaenhanced peritoneal cell epithelial-to-mesenchymal transition.
Long-term exposure to peritoneal dialysate with high glucose (HG) leads to peritoneal fibrosis and thus decreases dialysis efficiency. In this study, we explored the role of β-catenin in this process. C57BL/6 mice received daily intraperitoneal injection with 10% of the body weight of saline (control), 4.25% glucose peritoneal dialysis fluid (PDF), or PDF combined with 5 mg·kg-1of the β-catenin inhibitor ICG-001 (PDF+ICG) for 30 days. Also, mice peritoneal epithelial cells (mPECs) were cultured in 4.25% glucose (HG) or combined with 10 μm ICG-001 (HG+ICG) for 48 h. We found greater thickness of the parietal peritoneum in the PDF-treated mice. Additionally, lower expression of E-cadherin, higher expression of Vimentin, β-catenin, and Snail, and activation of β-catenin was observed in the mice and in HG-treated mPECs, all of which were reversed by ICG-001. The changes in E-cadherin and Vimentin indicated occurrence of the epithelial-to-mesenchymal transition (EMT). Thus, β-catenin signaling participates in the process of HG-induced peritoneal fibrosis, and the EMT of peritoneal epithelial cells is one of the underlying mechanisms of this pathological change.
Department of Nephrology The People's Hospital of Zhejiang Province Hangzhou China.
Shuiyu
S
Ji
Kidney Disease Center The First Affiliated Hospital Zhejiang University Hangzhou China.
Hao
H
Deng
Department of Nephrology The First People's Hospital of Tongxiang China.
Wei
W
Jin
Kidney Disease Center The First Affiliated Hospital Zhejiang University Hangzhou China.
Pengpeng
P
Yan
Kidney Disease Center The First Affiliated Hospital Zhejiang University Hangzhou China.
Rending
R
Wang
Kidney Disease Center The First Affiliated Hospital Zhejiang University Hangzhou China.
Lisha
L
Pang
Kidney Disease Center The First Affiliated Hospital Zhejiang University Hangzhou China.
Jingyi
J
Zhou
Department of Nephrology The People's Hospital of Zhejiang Province Hangzhou China.
Jiaming
J
Zhang
Kidney Disease Center The First Affiliated Hospital Zhejiang University Hangzhou China.
Xiaoying
X
Chen
Department of Nephrology The People's Hospital of Zhejiang Province Hangzhou China.
Xiang
X
Zhao
Kidney Disease Center The First Affiliated Hospital Zhejiang University Hangzhou China.
Jia
J
Shen
epithelial‐to‐mesenchymal transition; high glucose; peritoneal dialysis; peritoneal fibrosis; β‐catenin