GABA and GABA receptors alterations in the primary visual cortex of concave lens-induced myopic model.
Until recently most researches on myopia mechanisms have mainly been focused on the eye ball and few investigations were explored on the upper visual pathway, such as the visual cortex. The roles of gamma-aminobutyric acid (GABA) in the retinal and in the upper visual pathway are inter-correlated. As the retinal glutamate decarboxylase (GAD), GABA, and the mRNA levels of GABA receptors increased during the concave lens induced myopia formation, however, whether GABA alterations also occurred in the visual cortex during the concave lens induction is still unknown. In the present study, using HPLC, Enzyme-Linked Immunosorbent Assay (ELISA) and Real-Time Quantitative-PCR (RT-PCR) methods, we observed the changing trends of GABA, glutamate decarboxylase (GAD), and GABA receptors in the visual cortex of concave lens-induced myopic guinea pigs. Similar to the changing patterns of retinal GABA, the concentrations of GAD, GABA and the mRNA levels of GABA receptors in the visual cortex also increased. These results indicate that the exploration on myopia mechanisms should possibly be investigated on the whole visual pathway and the detailed significance of cortical GABA alterations needs further investigation.
Shandong University of Traditional Chinese Medicine, Jinan, Shandong, People's Republic of China.
Wen
W
Zhao
Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases of Shandong, Eye Institute of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, People's Republic of China.
Ai-Ling
AL
Bi
Shandong University of Traditional Chinese Medicine, Jinan, Shandong, People's Republic of China; Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, People's Republic of China.
Chao-Li
CL
Xu
Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases of Shandong, Eye Institute of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, People's Republic of China.
Xiang
X
Ye
Graduate student of grade 2015, the Second clinical medicine school, Shandong University of Traditional Chinese Medicine, People's Republic of China.
Mei-Qing
MQ
Chen
School of Public Health, Shandong University, Jinan, Shandong, People's Republic of China.
Xin-Ting
XT
Wang
Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, People's Republic of China.
Xiao-Yan
XY
Zhang
Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases of Shandong, Eye Institute of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, People's Republic of China.
Jun-Guo
JG
Guo
Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases of Shandong, Eye Institute of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, People's Republic of China.
Wen-Jun
WJ
Jiang
Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, People's Republic of China.
Jin
J
Zhang
Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases of Shandong, Eye Institute of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, People's Republic of China; Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, People's Republic of China. Electronic address: hongshengbi1@163.com.
Hong-Sheng
HS
Bi
D000818:Animals; D004195:Disease Models, Animal; D005968:Glutamate Decarboxylase / Q000378:metabolism; D006168:Guinea Pigs; D008297:Male; D009216:Myopia / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D018079:Receptors, GABA / Q000378:metabolism*; D014793:Visual Cortex / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D012333:RNA, Messenger; D018079:Receptors, GABA; D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase
Concave lens-induced myopia; Gamma-amino butyric acid; Visual cortex