Simultaneous determination of eight bioactive components of Qishen Yiqi dripping pills in rat plasma using UFLC-MS/MS and its application to a pharmacokinetic study.
In this study, a rapid and reliable ultra-fast liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous determination of eight active ingredients, including astragaloside IV, ononin, tanshinol, protocatechualdehyde, protocatechuic acid, salvianolic acid D, rosmarinic acid and ginsenoside Rg1, in rat plasma. The plasma samples were pretreated by protein precipitation with acetonitrile. Chromatographic separation was performed on a Waters Acquity UPLC® BEH C18column (1.7 μm particles, 2.1 × 100 mm). The mobile phase consisted of 0.1% aqueous formic acid (A)-acetonitrile with 0.1% formic acid (B) at a flow rate of 0.4 mL/min. Quantification was performed on a triple quadruple tandem mass spectrometry with electrospray ionization by multiple reaction monitoring both in the negative and in the positive ion mode. The lower limit of quantification of tanshinol was 2.0 ng/mL and the others were 5.0 ng/mL. The extraction recoveries, matrix effects, intra- and inter-day precision and accuracy of eight tested components were all within acceptable limits. The validated method was successfully applied to the pharmacokinetic study of the eight active constituents after intragastric administration of three doses (1.0, 3.0, 6.0 g/kg body weight) of Qishen Yiqi Dripping Pills to rats.
School of Pharmacy, China Pharmaceutical University, Nanjing, People's Republic of China.
Yaping
Y
Shao
Tasly Academy, Tianjin Tasly Holding Group Co. Ltd, Tianjin, People's Republic of China.|Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, People's Republic of China.|Research and Development Center of TCM, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, People's Republic of China.
Wen
W
Zhang
Tasly Academy, Tianjin Tasly Holding Group Co. Ltd, Tianjin, People's Republic of China.
Ling
L
Tong
Tasly Academy, Tianjin Tasly Holding Group Co. Ltd, Tianjin, People's Republic of China.
Jingyi
J
Huang
Tasly Academy, Tianjin Tasly Holding Group Co. Ltd, Tianjin, People's Republic of China.
Dongxiang
D
Li
Tasly Academy, Tianjin Tasly Holding Group Co. Ltd, Tianjin, People's Republic of China.
Wei
W
Nie
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, People's Republic of China.|Research and Development Center of TCM, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, People's Republic of China.
Yan
Y
Zhu
Tasly Academy, Tianjin Tasly Holding Group Co. Ltd, Tianjin, People's Republic of China.
Yunfei
Y
Li
School of Pharmacy, China Pharmaceutical University, Nanjing, People's Republic of China.
Tao
http://orcid.org/0000-0002-3759-4354
T
Lu
D000475:Alkenes / Q000032:analysis / Q000097:blood; D000818:Animals; D001547:Benzaldehydes / Q000032:analysis / Q000097:blood; D002109:Caffeic Acids / Q000032:analysis / Q000097:blood; D002396:Catechols / Q000032:analysis / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002934:Cinnamates / Q000032:analysis / Q000097:blood; D053630:Depsides / Q000032:analysis / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000493:pharmacokinetics*; D036145:Ginsenosides / Q000032:analysis / Q000097:blood; D005960:Glucosides / Q000032:analysis / Q000097:blood; D062385:Hydroxybenzoates / Q000032:analysis / Q000097:blood; D007529:Isoflavones / Q000032:analysis / Q000097:blood; D057230:Limit of Detection; D008297:Male; D059808:Polyphenols / Q000032:analysis / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000032:analysis / Q000097:blood; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014315:Triterpenes / Q000032:analysis / Q000097:blood; D000097123:Rosmarinic Acid
D000475:Alkenes; D001547:Benzaldehydes; D002109:Caffeic Acids; D002396:Catechols; D002934:Cinnamates; D053630:Depsides; D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D005960:Glucosides; D062385:Hydroxybenzoates; D007529:Isoflavones; D059808:Polyphenols; D012503:Saponins; D014315:Triterpenes; D000097123:Rosmarinic Acid; C526426:calycosin-7-O-beta-D-glucoside; C528580:qishen yiqi; C568740:salvianolic acid; C585969:tanshinol; C009091:protocatechuic acid; C052064:astragaloside A; C005581:protocatechualdehyde; C035054:ginsenoside Rg1
Qishen Yiqi dripping pills; UFLC-MS/MS; pharmacokinetics; rat plasma