Mapping cortical mesoscopic networks of single spiking cortical or sub-cortical neurons.
Understanding the basis of brain function requires knowledge of cortical operations over wide-spatial scales, but also within the context of single neurons. In vivo, wide-field GCaMP imaging and sub-cortical/cortical cellular electrophysiology were used in mice to investigate relationships between spontaneous single neuron spiking and mesoscopic cortical activity. We make use of a rich set of cortical activity motifs that are present in spontaneous activity in anesthetized and awake animals. A mesoscale spike-triggered averaging procedure allowed the identification of motifs that are preferentially linked to individual spiking neurons by employing genetically targeted indicators of neuronal activity. Thalamic neurons predicted and reported specific cycles of wide-scale cortical inhibition/excitation. In contrast, spike-triggered maps derived from single cortical neurons yielded spatio-temporal maps expected for regional cortical consensus function. This approach can define network relationships between any point source of neuronal spiking and mesoscale cortical maps.
Department of Psychiatry, Kinsmen Laboratory of Neurological Research, Vancouver, Canada.|Beijing Institute for Brain Disorders, Capital Medical University, Beijing, China.|Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, Canada.
Dongsheng
D
Xiao
Department of Psychiatry, Kinsmen Laboratory of Neurological Research, Vancouver, Canada.|Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, Canada.
Matthieu P
MP
Vanni
Department of Psychiatry, Kinsmen Laboratory of Neurological Research, Vancouver, Canada.|Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, Canada.
Catalin C
CC
Mitelut
Department of Psychiatry, Kinsmen Laboratory of Neurological Research, Vancouver, Canada.|Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, Canada.
Allen W
AW
Chan
Department of Psychiatry, Kinsmen Laboratory of Neurological Research, Vancouver, Canada.|Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, Canada.
Jeffrey M
JM
LeDue
Department of Psychiatry, Kinsmen Laboratory of Neurological Research, Vancouver, Canada.|Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, Canada.
Yicheng
Y
Xie
Beijing Institute for Brain Disorders, Capital Medical University, Beijing, China.
Andrew Cn
AC
Chen
Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, Canada.
Nicholas V
NV
Swindale
Department of Psychiatry, Kinsmen Laboratory of Neurological Research, Vancouver, Canada.|Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, Canada.
Timothy H
0000-0002-0093-4490
TH
Murphy
D000200:Action Potentials / Q000502:physiology*; D000758:Anesthesia; D000818:Animals; D001931:Brain Mapping; D002118:Calcium / Q000502:physiology; D020013:Calcium Signaling / Q000502:physiology; D002540:Cerebral Cortex / Q000033:anatomy & histology / Q000502:physiology*; D004567:Electrodes, Implanted; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D015335:Molecular Probes / Q000737:chemistry / Q000235:genetics; D009415:Nerve Net / Q000033:anatomy & histology / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D061848:Optical Imaging / Q000379:methods; D013238:Stereotaxic Techniques; D013788:Thalamus / Q000033:anatomy & histology / Q000502:physiology*; D014851:Wakefulness / Q000502:physiology
D015335:Molecular Probes; D002118:Calcium
functional connectivity; mesoscale maps; mouse; neuroscience; spike triggered maps; thalamocortical