Extraction and purification of pumpkin polysaccharides and their hypoglycemic effect.
The anti-diabetic activity of the polysaccharides (PPs) obtained from the dried pumpkin pulp was studied in this paper. The PPs were administered by intraperitoneal injection to the alloxan-induced diabetic male ICR mice. The PPs hypoglycemic effect was evaluated by testing the fast blood glucose level, fasting serum insulin and hepatic glycogen. After 7h administration, the PPs showed a significantly hypoglycemic effect (p<0.01) and could significantly increase hepatic glycogen and insulin level (p<0.05). PPs-e with potential to enhance the islet cells proliferation activity by MTT assays in vitro was obtained from the PPs through alcohol fractional precipitation and gel chromatography. PPs-e composed of rhamnose, arabinose, glucose, galactose, and little amount of inositol could maintain the blood glucose at a low level in diabetic mice and could even last for more than 24h. These results suggest the potential hypoglycemic effect of the pumpkin polysaccharides in alloxan-induced diabetic mice and for the treatment of diabetic mellitus.
College of Life Sciences, China JiLiang University, Hangzhou, Zhejiang, 310018, China.
Shuang
S
Wang
College of Life Sciences, China JiLiang University, Hangzhou, Zhejiang, 310018, China.
Aoxue
A
Lu
College of Life Sciences, China JiLiang University, Hangzhou, Zhejiang, 310018, China.
Lu
L
Zhang
College of Life Sciences, China JiLiang University, Hangzhou, Zhejiang, 310018, China.
Meng
M
Shen
College of Life Sciences, China JiLiang University, Hangzhou, Zhejiang, 310018, China.
Tian
T
Xu
College of Life Sciences, China JiLiang University, Hangzhou, Zhejiang, 310018, China.
Wangyang
W
Zhan
College of Life Sciences, China JiLiang University, Hangzhou, Zhejiang, 310018, China.
Hui
H
Jin
College of Life Sciences, China JiLiang University, Hangzhou, Zhejiang, 310018, China. Electronic address: yjzhang@vip.163.com.
Yongjun
Y
Zhang
College of Life Sciences, China JiLiang University, Hangzhou, Zhejiang, 310018, China.
Weimin
W
Wang
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D028464:Cucurbita / Q000737:chemistry*; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D005215:Fasting / Q000097:blood; D006003:Glycogen / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology* / Q000627:therapeutic use; D007328:Insulin / Q000097:blood; D007515:Islets of Langerhans / Q000187:drug effects / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology* / Q000627:therapeutic use
D001786:Blood Glucose; D007004:Hypoglycemic Agents; D007328:Insulin; D011134:Polysaccharides; D006003:Glycogen
Anti-diabetic effects; Proliferation activity; Pumpkin polysaccharide