Use of chemometrics to optimize a glucose assay on a paper microfluidic platform.
We describe the use of a chemometrics-based computational platform to optimize a glucose assay on a microfluidic paper-based analytical device (μPAD). Glucose is colorimetrically detected in the presence of glucose oxidase (GOx), horseradish peroxidase (HRP), and potassium iodide (KI). Using a Y-shaped paper microfluidic chip, the concentration of glucose, volume of reagents, and the length and width of the microfluidic channel were examined. The responses of the microfluidic chips were analyzed at the halfway point of the channel length. Variables affecting the response were screened by using a 24factorial design, and among them, volume and concentration of the glucose were optimized by applying a rotatable central composite design (CCD). The optimum and experimental responses are 151.58 and 149.80, respectively, with an absolute error of 1.2%.
Department of Chemistry and Biochemistry, California State University, 5151 State University Drive, Los Angeles, CA, 90032-8202, USA.
Ani
A
Avoundjian
Department of Chemistry and Biochemistry, California State University, 5151 State University Drive, Los Angeles, CA, 90032-8202, USA.
Mehdi
M
Jalali-Heravi
Department of Chemistry and Biochemistry, California State University, 5151 State University Drive, Los Angeles, CA, 90032-8202, USA. fgomez2@calstatela.edu.
Frank A
FA
Gomez
D001681:Biological Assay / Q000379:methods*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D010209:Paper
D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D005947:Glucose
Chemometrics; Glucose oxidase; Paper microfluidics; Point-of-care diagnostic device