Effects of spermidine and ATP on stabilities of chromatosomes and histone H1-depleted chromatosomes.
It is shown in our FRET studies that both chromatosomes and histone H1-depleted chromatosomes exist in their arm-closed forms in the absence of spermidine. In the presence of spermidine, however, these two types of structural assemblies are converted into their arm-open forms. In addition, ATP as polyanion is capable of suppressing the polycationic effect of spermidine, thus facilitating re-formation of arm-closed forms of these two types of structural assemblies. Our studies therefore illustrate that conversion between arm-closed and arm-open forms of chromatosomes and histone H1-depleted chromatosomes can be manipulated by varying concentrations of polycationic spermidine and polyanionic ATP.
Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore 637371, Singapore.
Hao
H
Zhang
Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore 637371, Singapore. Electronic address: thli@ntu.edu.sg.
Tianhu
T
Li
D000255:Adenosine Triphosphate / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer; D015394:Molecular Structure; D013095:Spermidine / Q000378:metabolism*
D002843:Chromatin; D000255:Adenosine Triphosphate; D013095:Spermidine
Chromatosome; FRET; Histone H1; Histone octamer; Spermidine