Evaluation of intestinal damage caused by V. cholerae O139, an in vivo study.
Cholera, a severe form of gastroenteritis, is one of the most widespread diseases in developing countries. The mechanism of intestinal infection caused by V. cholerae O139 remains unclear. In order to explore some morphological aspects of its infection in the intestine including Peyer's patches, we investigated the V. cholerae O139 infection at intestinal site of the rabbit gut-loop model. The electron microscopic analysis revealed denuded mucosal surface with loss of microvilli and integrity of the surface epithelium. Infection of the intestine with V. cholerae O139 induces destruction of villi, microvilli and lining epithelium with exposure of crypts of Lieberkuhn.
Department of Biomedical Sciences and Therapeutics, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia; School of Health Sciences, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia. Electronic address: chandri20@yahoo.co.uk.
Chandrika
C
Murugaiah
Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia.
Nik Zuraina Nik Mohd
NZ
Noor
Pathology Unit, Faculty of Medicine, AIMST University, 08100 Bedong, Kedah, Malaysia.
Shyamoli
S
Mustafa
Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Semeling, 08100 Bedong, Kedah, Malaysia.
Ravichandran
R
Manickam
Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Semeling, 08100 Bedong, Kedah, Malaysia.
Lalitha
L
Pattabhiraman
D000818:Animals; D002771:Cholera / Q000382:microbiology* / Q000473:pathology*; D004195:Disease Models, Animal; D007422:Intestines / Q000382:microbiology* / Q000473:pathology*; D008855:Microscopy, Electron, Scanning; D011817:Rabbits; D041242:Vibrio cholerae O139 / Q000472:pathogenicity*
Cholera; Infection; V. cholerae O139; Vibrio