A two-column flash chromatography approach to pyoverdin production from Pseudomonas putida GB1.
Our knowledge of the biological and environmental reactivity of siderophores is limited by the difficulty and cost of obtaining reasonable quantities by purification or synthesis. In this note, we describe a modified procedure for the low-cost, mg-scale purification of pyoverdin-type siderophores using a dual-flash chromatography (reverse-phase absorption and size exclusion) approach.
Department of Crop and Soil Sciences, North Carolina State University, Raleigh, NC 27695, USA. Electronic address: owen_duckworth@ncsu.edu.
Owen W
OW
Duckworth
Department of Crop and Soil Sciences, North Carolina State University, Raleigh, NC 27695, USA.
Dawn S
DS
Markarian
Scripps Institution of Oceanography, University of California, La Jolla, San Diego, CA 92093, USA.
Dorothy L
DL
Parker
RTI International, 3040 East Cornwallis Road, Durham, NC 27709, USA.
James M
JM
Harrington
D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D013058:Mass Spectrometry; D009842:Oligopeptides / Q000737:chemistry* / Q000302:isolation & purification*; D016958:Pseudomonas putida / Q000378:metabolism*; D017262:Siderophores / Q000737:chemistry / Q000302:isolation & purification
D009842:Oligopeptides; D017262:Siderophores; C042453:pyoverdin
Flash chromatography; Purification; Siderophores