Postembryonic Fish Brain Proliferation Zones Exhibit Neuroepithelial-Type Gene Expression Profile.
In mammals, neuroepithelial cells play an essential role in embryonic neurogenesis, whereas glial stem cells are the principal source of neurons at postembryonic stages. By contrast, neuroepithelial-like stem/progenitor (NE) cells have been shown to be present throughout life in teleosts. We used three-dimensional (3D) reconstructions of cleared transgenic wdr12:GFP medaka brains to demonstrate that this cell type is widespread in juvenile and to identify new regions containing NE cells. We established the gene expression profile of optic tectum (OT) NE cells by cell sorting followed by RNA-seq. Our results demonstrate that most OT NE cells are indeed active stem cells and that some of them exhibit long G2 phases. We identified several novel pathways (e.g., DNA repair pathways) potentially involved in NE cell homeostasis. In situ hybridization studies showed that all NE populations in the postembryonic medaka brain have a similar molecular signature. Our findings highlight the importance of NE progenitors in medaka and improve our understanding of NE-cell biology. These cells are potentially useful not only for neural stem cell studies but also for improving the characterization of neurodevelopmental diseases, such as microcephaly. Stem Cells 2017;35:1505-1518.
INRA CASBAH Group, Neuro-PSI, UMR 9197, CNRS, Gif-sur-Yvette, France.
Emilie
E
Dambroise
INRA CASBAH Group, Neuro-PSI, UMR 9197, CNRS, Gif-sur-Yvette, France.
Matthieu
M
Simion
BIOS UMR 7247, INRA PRC, Nouzilly, France.
Thomas
T
Bourquard
INRA CASBAH Group, Neuro-PSI, UMR 9197, CNRS, Gif-sur-Yvette, France.
Stéphanie
S
Bouffard
Tefor Core Facility, TEFOR Infrastructure, Neuro-PSI, CNRS, Gif-sur-Yvette, France.
Barbara
B
Rizzi
Plateforme de séquençage haut débit.
Yan
Y
Jaszczyszyn
Plateforme de cytométrie, I2BC.
Mickaël
M
Bourge
Tefor Core Facility, TEFOR Infrastructure, Neuro-PSI, CNRS, Gif-sur-Yvette, France.
Pierre
P
Affaticati
INRA CASBAH Group, Neuro-PSI, UMR 9197, CNRS, Gif-sur-Yvette, France.
Aurélie
A
Heuzé
Plateforme BM-Gif, Imagif, UMR 9198, CNRS, Gif-sur-Yvette, France.
Julia
J
Jouralet
UMS AMAGEN CNRS, INRA, Université Paris-Saclay, Gif-sur-Yvette, France.
Joanne
J
Edouard
Plateforme de cytométrie, I2BC.
Spencer
S
Brown
Plateforme de séquençage haut débit.
Claude
C
Thermes
BIOS UMR 7247, INRA PRC, Nouzilly, France.
Anne
A
Poupon
BIOS UMR 7247, INRA PRC, Nouzilly, France.
Eric
E
Reiter
UMS AMAGEN CNRS, INRA, Université Paris-Saclay, Gif-sur-Yvette, France.
Frédéric
F
Sohm
INRA CASBAH Group, Neuro-PSI, UMR 9197, CNRS, Gif-sur-Yvette, France.
Franck
F
Bourrat
INRA CASBAH Group, Neuro-PSI, UMR 9197, CNRS, Gif-sur-Yvette, France.
Jean-Stéphane
JS
Joly
D000818:Animals; D030801:Animals, Genetically Modified; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000166:cytology* / Q000254:growth & development*; D049109:Cell Proliferation / Q000235:genetics; D004260:DNA Repair / Q000235:genetics; D016195:G2 Phase; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000378:metabolism; D046569:Neuroepithelial Cells / Q000378:metabolism*; D009990:Oryzias / Q000235:genetics / Q000254:growth & development*; D017423:Sequence Analysis, RNA; D013477:Superior Colliculi / Q000166:cytology; D015854:Up-Regulation
D015415:Biomarkers; D049452:Green Fluorescent Proteins
Cell cycle; DNA repair; Microcephaly; Neuroepithelial cell; Optic tectum; RNA seq