Diamond-based electrochemical aptasensor realizing a femtomolar detection limit of bisphenol A.
In this study, we designed and fabricated an electrochemical impedance aptasensor based on Au nanoparticles (Au-NPs) coated boron-doped diamond (BDD) modified with aptamers, and 6-mercapto-1-hexanol (MCH) for the detection of bisphenol A (BPA). The constructed BPA aptasensor exhibits good linearity from 1.0×10-14to 1.0×10-9molL-1. The detection limitation of 7.2×10-15molL-1was achieved, which can be attributed to the synergistic effect of combining BDD with Au-NPs, aptamers, and MCH. The examine results of BPA traces in Tris-HCl buffer and in milk, UV spectra of aptamer/BPA and interference test revealed that the novel aptasensors are of high sensitivity, specificity, stability and repeatability, which could be promising in practical applications.
State Key Laboratory of Superhard Materials, Jilin University, Changchun 130012, PR China.
Yibo
Y
Ma
State Key Laboratory of Superhard Materials, Jilin University, Changchun 130012, PR China.
Junsong
J
Liu
State Key Laboratory of Superhard Materials, Jilin University, Changchun 130012, PR China. Electronic address: hdli@jlu.edu.cn.
Hongdong
H
Li
D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001559:Benzhydryl Compounds / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D001895:Boron / Q000737:chemistry; D018130:Diamond / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods; D052244:Endocrine Disruptors / Q000032:analysis*; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D008892:Milk / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010636:Phenols / Q000032:analysis*; D000098826:Bisphenol A Compounds
D052157:Aptamers, Nucleotide; D001559:Benzhydryl Compounds; D001895:Boron; D018130:Diamond; D052244:Endocrine Disruptors; D006046:Gold; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
Bisphenol A; Boron-doped diamond (BDD); Electrochemical aptasensor; Femtomolar detection limit; MCH/Aptamer/Au nanoparticles/BDD