Over-expression of SlJA2 decreased heat tolerance of transgenic tobacco plants via salicylic acid pathway.
Over-expression of SlJA2 decreased the accumulation of SA, which resulted in significant physiological and gene expression changes in transgenic tobacco plants, leading to the decreased heat tolerance of transgenic tobacco. NAC family, the largest transcription factors in plants, responses to different environmental stimuli. Here, we isolated a typical NAC transcription factor (SlJA2) from tomato and got transgenic tobacco with SlJA2 over-expression. Expression of SlJA2 was induced by heat stress (42 °C), chilling stress (4 °C), drought stress, osmotic stress, abscisic acid, and salicylic acid. Over-expression of SlJA2 decreased the accumulation of salicylic acid by regulating expression of salicylic acid degradation gene under heat stress. Compared to WT plants, stomatal apertures and water loss increased in transgenic plants, and the damage of photosynthetic apparatus and chlorophyll breakdown were more serious in transgenic plants under heat stress. Meanwhile, more H2O2and O2·-were accumulated transgenic plants and proline synthesis was restricted, which resulted in more serious oxidative damage compared to WT. qRT-PCR analysis showed that over-expression of SlJA2 could down-regulate genes involved in reactive oxygen species scavenging, proline biosynthesis, and response to heat stress. All the above results indicated that SlJA2 may be a negative regulator responded to plant's heat tolerance. Thus, this study provides new insight into roles of NAC family member in plant response to abiotic stress.
College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, 271018, Shandong, People's Republic of China.
Zhong-Ming
ZM
Liu
College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, 271018, Shandong, People's Republic of China.
Meng-Meng
MM
Yue
College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, 271018, Shandong, People's Republic of China.
Dong-Yue
DY
Yang
College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, 271018, Shandong, People's Republic of China.
Shao-Bo
SB
Zhu
College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, 271018, Shandong, People's Republic of China. nnma@sdau.edu.cn.
Na-Na
NN
Ma
College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, 271018, Shandong, People's Republic of China. qwmeng@sdau.edu.cn.
Qing-Wei
QW
Meng
D055864:Droughts; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D006861:Hydrogen Peroxide / Q000378:metabolism; D018551:Solanum lycopersicum / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics*; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics*; D000071436:Thermotolerance / Q000235:genetics*; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
D010940:Plant Proteins; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D020156:Salicylic Acid
Heat stress; SA; SlJA2; Stomatal; Transgenic tobacco