Characterization of Polysaccharides with Antioxidant and Hepatoprotective Activities from the Edible Mushroom Oudemansiella radicata.
The preliminary structure, in vitro antioxidant and in vivo hepatoprotective activities of water-soluble polysaccharides (ORWP) and alkali-soluble polysaccharides (ORAP), prepared from the mushroomOudemansiella radicata, were investigated. Both ORWP and ORAP were heteropolysaccharides with mannose, glucose and galactose being the main monosaccharide components. Regarding the antioxidant activities, ORWP and ORAP showed effective 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, hydrogen peroxide scavenging activity and lipid peroxidation inhibitory effects, as well as moderate reducing power and Fe2+chelating activity. For the hepatoprotective activity, administration of ORWP and ORAP prevented the increase in serum alanine aminotransferase and aspartate aminotransferase activities in a carbon tetrachloride-induced acute liver damage model, suppressed hepatic malondialdehyde formation and stimulated the activities of hepatic superoxide dismutase and glutathione peroxidase. Thus, we speculate that ORWP and ORAP may protect the liver from CCl₄-induced hepatic damage via antioxidant mechanisms.
Institute of Plant Nutrition, Agricultural Resources and Environmental Science, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. liuqin_bio@hotmail.com.|State Key Laboratory for Agrobiotechnology, Department of Microbiology, China Agricultural University, Beijing 100193, China. liuqin_bio@hotmail.com.
Qin
Q
Liu
Department of Fungal Resource, College of Plant Protection, Shandong Agricultural University, Tai'an 271018, China. mengfan7777@163.com.
Mengjuan
M
Zhu
College of Food Science and Engineering, Shanxi Agricultural University, Taigu 030801, China. gengxueran2007@163.com.
Xueran
X
Geng
State Key Laboratory for Agrobiotechnology, Department of Microbiology, China Agricultural University, Beijing 100193, China. hxwang@cau.edu.cn.
Hexiang
H
Wang
School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong 999077, China. tzibunng@cuhk.edu.hk.
Tzi Bun
TB
Ng
D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D005609:Free Radicals / Q000037:antagonists & inhibitors; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000737:chemistry / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008111:Liver Function Tests; D051379:Mice; D010084:Oxidation-Reduction / Q000187:drug effects; D020011:Protective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared
D000975:Antioxidants; D005609:Free Radicals; D062610:Fungal Polysaccharides; D007502:Iron Chelating Agents; D020011:Protective Agents
Oudemansiella radicata; antioxidant; hepatoprotective; polysaccharides