Genome-Wide Association Study of Genetic Control of Seed Fatty Acid Biosynthesis inBrassica napus.
Fatty acids and their composition in seeds determine oil value for nutritional or industrial purposes and also affect seed germination as well as seedling establishment. To better understand the genetic basis of seed fatty acid biosynthesis in oilseed rape (Brassica napusL.) we applied a genome-wide association study, using 91,205 single nucleotide polymorphisms (SNPs) characterized across a mapping population with high-resolution skim genotyping by sequencing (SkimGBS). We identified a cluster of loci on chromosome A05 associated with oleic and linoleic seed fatty acids. The delineated genomic region contained orthologs of theArabidopsis thalianagenes known to play a role in regulation of seed fatty acid biosynthesis such as Fatty acyl-ACP thioesterase B (FATB) and Fatty Acid Desaturase (FAD5). This approach allowed us to identify potential functional genes regulating fatty acid composition in this important oil producing crop and demonstrates that this approach can be used as a powerful tool for dissecting complex traits forB. napusimprovement programs.
Plant Breeding and Acclimatization Institute-National Research Institute, Oilseed Crops Research Centre Poznan, Poland.
Katarzyna
K
Gacek
School of Plant Biology, University of Western Australia Perth, WA, Australia.
Philipp E
PE
Bayer
Plant Breeding and Acclimatization Institute-National Research Institute, Oilseed Crops Research Centre Poznan, Poland.
Iwona
I
Bartkowiak-Broda
Plant Breeding and Acclimatization Institute-National Research Institute, Oilseed Crops Research Centre Poznan, Poland.
Laurencja
L
Szala
Department of Mathematical and Statistical Methods, Poznan University of Life Sciences Poznan, Poland.
Jan
J
Bocianowski
School of Plant Biology, University of Western Australia Perth, WA, Australia.
David
D
Edwards
School of Plant Biology, University of Western Australia Perth, WA, Australia.
Jacqueline
J
Batley
Brassica napus; GWAS; fatty acid biosynthesis; seed quality; skim genotyping by sequencing (Skim GBS)