Staining of Lipid Droplets with Monodansylpentane.
Monodansylpentane (MDH) is a fluorophore that displays selective labeling of lipid droplets (LDs). The dye preferentially segregates into the neutral lipid cores of LDs and emits blue fluorescence, compatible with the simultaneous use of green and red fluorescent reporters in multi-color live-cell imaging. MDH can be used for visualizing LDs not only in cell cultures, but also in fixed tissues such as the fat body and ovaries from Drosophila. MDH is therefore a versatile marker for LDs in fluorescence microscopy.
Chemical Biology and Molecular Biophysics Program, Taiwan International Graduate Program, Taipei, Taiwan.|Institute of Biological Chemistry, Academia Sinica, 128 Academia Road Sec. 2, Nankang, Taipei, 115, Taiwan.|Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu, Taiwan.
Bo-Hua
BH
Chen
Institute of Biological Chemistry, Academia Sinica, 128 Academia Road Sec. 2, Nankang, Taipei, 115, Taiwan.
Huei-Jiun
HJ
Yang
Institute of Biological Chemistry, Academia Sinica, 128 Academia Road Sec. 2, Nankang, Taipei, 115, Taiwan.
He-Yen
HY
Chou
Chemical Biology and Molecular Biophysics Program, Taiwan International Graduate Program, Taipei, Taiwan.|Institute of Biological Chemistry, Academia Sinica, 128 Academia Road Sec. 2, Nankang, Taipei, 115, Taiwan.|Institute of Biochemical Sciences, College of Life Sciences, National Taiwan University, Taipei, Taiwan.
Guang-Chao
GC
Chen
Chemical Biology and Molecular Biophysics Program, Taiwan International Graduate Program, Taipei, Taiwan. weiyang@gate.sinica.edu.tw.|Institute of Biological Chemistry, Academia Sinica, 128 Academia Road Sec. 2, Nankang, Taipei, 115, Taiwan. weiyang@gate.sinica.edu.tw.|Institute of Biochemical Sciences, College of Life Sciences, National Taiwan University, Taipei, Taiwan. weiyang@gate.sinica.edu.tw.
Wei Yuan
WY
Yang
D000818:Animals; D002460:Cell Line; D003619:Dansyl Compounds; D004330:Drosophila; D005456:Fluorescent Dyes; D006801:Humans; D066292:Lipid Droplets; D008856:Microscopy, Fluorescence / Q000379:methods; D013194:Staining and Labeling / Q000379:methods
D003619:Dansyl Compounds; D005456:Fluorescent Dyes; C573695:monodansylpentane
Drosophila; Fluorescence microscopy; Lipid droplets; Live-cell imaging; Monodansylpentane; Tissue