Tumor Grade versus Expression of Invasion-Related Molecules in Astrocytoma.
Peritumoral infiltration is characteristic of astrocytomas even in low-grade tumors. Tumor cells migrate to neighbouring tissue and cause recurrence. The extracellular matrix (ECM) plays a role in tumor invasion; expression levels of its components' have been linked to tumor invasion. This study determines the mRNA and protein expression of 20 invasion-related ECM components by examining non-tumor brain; grade I-II-III astrocytoma and glioblastoma samples. Expression levels were measured by QRT-PCR and mass-spectroscopy. The connection between the expression pattern and tumor grade is statistically analyzed. During the analysis of data, key molecules (brevican, cadherin-12, fibronectin and integrin-β1) correlating the most with tumor grade were selected. While the mRNA level of brevican, ErbB2, fibronectin, integrin-β1 and versican discriminates low-grade from high-grade gliomas, of proteins RHAMM, integrin-α1 and MMP2 seems important. The expression pattern was found to be distinctive for tumor grade, as statistical classifiers are capable of identifying an unknown sample's grade using them. Furthermore, normal brain and glioma expression patterns, along with low-grade astrocytoma and glioblastoma samples, differ the most. Determining the invasion-related molecules' expression profile provides extra information regarding the tumor's clinical behavior. Additionally, identifying molecules playing a key role in glioma invasion could uncover potential therapeutic targets in the future.
Department of Neurosurgery, University of Debrecen Clinical Center, Nagyerdei krt. 98, Debrecen, 4032, Hungary.
József
J
Virga
Department of Neurosurgery, University of Debrecen Clinical Center, Nagyerdei krt. 98, Debrecen, 4032, Hungary.
László
L
Bognár
Department of Pathology, University of Debrecen Clinical Center, Nagyerdei krt. 98, Debrecen, 4032, Hungary.
Tibor
T
Hortobágyi
UD-GenoMed Medical Genomic Technologies Research & Development Services Ltd., Nagyerdei krt. 98, Debrecen, 4032, Hungary.
Gábor
G
Zahuczky
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, Debrecen, 4032, Hungary.
Éva
É
Csősz
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, Debrecen, 4032, Hungary.
Gergő
G
Kalló
Department of Oncology, University of Debrecen Clinical Center, Nagyerdei krt. 98, Debrecen, 4032, Hungary.
Judit
J
Tóth
Department of Neurosurgery, University of Debrecen Clinical Center, Nagyerdei krt. 98, Debrecen, 4032, Hungary.
Gábor
G
Hutóczki
Department of Neurosurgery, University of Debrecen Clinical Center, Nagyerdei krt. 98, Debrecen, 4032, Hungary.
Judit
J
Reményi-Puskár
UD-GenoMed Medical Genomic Technologies Research & Development Services Ltd., Nagyerdei krt. 98, Debrecen, 4032, Hungary.
László
L
Steiner
Department of Neurosurgery, University of Debrecen Clinical Center, Nagyerdei krt. 98, Debrecen, 4032, Hungary. neurosurgery.debrecen@freemail.hu.
Almos
A
Klekner
D001254:Astrocytoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D016022:Case-Control Studies; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*
D014408:Biomarkers, Tumor; D012333:RNA, Messenger
Expression; Extracellular matrix; Gliomas; Invasion; Tumor grade