Evolutionary origins of the emergent ST796 clone of vancomycin resistantEnterococcus faecium.
From early 2012, a novel clone of vancomycin resistantEnterococcus faecium(assigned the multi locus sequence type ST796) was simultaneously isolated from geographically separate hospitals in south eastern Australia and New Zealand. Here we describe the complete genome sequence of Ef_aus0233, a representative ST796E. faeciumisolate. We used PacBio single molecule real-time sequencing to establish a high quality, fully assembled genome comprising a circular chromosome of 2,888,087 bp and five plasmids. Comparison of Ef_aus0233 to otherE. faeciumgenomes shows Ef_aus0233 is a member of the epidemic hospital-adapted lineage and has evolved from an ST555-like ancestral progenitor by the accumulation or modification of five mosaic plasmids and five putative prophage, acquisition of two cryptic genomic islands, accrued chromosomal single nucleotide polymorphisms and a 80 kb region of recombination, also gaining Tn1549and Tn916, transposons conferring resistance to vancomycin and tetracycline respectively. The genomic dissection of this new clone presented here underscores the propensity of the hospitalE. faeciumlineage to change, presumably in response to the specific conditions of hospital and healthcare environments.
Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne , Melbourne , Victoria , Australia.
Andrew H
AH
Buultjens
Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne , Melbourne , Victoria , Australia.
Margaret M C
MM
Lam
Microbiology Diagnostic Unit, Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne , Melbourne , Victoria , Australia.
Susan
S
Ballard
Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne , Melbourne , Victoria , Australia.
Ian R
IR
Monk
Infectious Diseases Department, Austin Health , Heidelberg , Victoria , Australia.
Andrew A
AA
Mahony
Infectious Diseases Department, Austin Health , Heidelberg , Victoria , Australia.
Elizabeth A
EA
Grabsch
Infectious Diseases Department, Austin Health , Heidelberg , Victoria , Australia.
M Lindsay
ML
Grayson
School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, Australia; Department of Microbiology, Pathwest Laboratory Medicine-WA, Fiona Stanley Hospital, Murdoch, Western Australia, Australia.
Stanley
S
Pang
School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, Australia; Department of Microbiology, Pathwest Laboratory Medicine-WA, Fiona Stanley Hospital, Murdoch, Western Australia, Australia.
Geoffrey W
GW
Coombs
School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, Australia; Department of Infectious Diseases, Fiona Stanley Hospital, Murdoch, Western Australia, Australia.
J Owen
JO
Robinson
Victorian Life Sciences Computation Initiative, University of Melbourne , Carlton , Victoria , Australia.
Torsten
T
Seemann
Infectious Diseases Department, Austin Health, Heidelberg, Victoria, Australia; Department of Medicine, University of Melbourne, Heidelberg, Victoria, Australia.
Paul D R
PD
Johnson
Microbiology Diagnostic Unit, Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne , Melbourne , Victoria , Australia.
Benjamin P
BP
Howden
Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne , Melbourne , Victoria , Australia.
Timothy P
TP
Stinear
Accessory genome; Antibiotic resistance; Comparative genomics; Enterococcus faecium; Evolution; Genome sequence; Hospital adapted; PacBio; Recombination; Vancomycin resistant