Tetradecanol reduces EL-4 T cell growth by the down regulation of NF-κB mediated IL-2 secretion.
Tetradecanol is a straight-chain saturated fatty alcohol purified from Dendropanax morbifera leaves. We found that tetradecanol (30μM) reduced specifically the growth of T cells such as EL-4 T cell and isolated murine CD4+T cells. In this study, we investigated the effects of tetradecanol on the regulation of interlukin-2 (IL-2), a potent T cell growth factor. Tetradecanol significantly inhibited IL-2 secretion in EL-4 T cells activated with phorbol 12-myristate 13-acetate (PMA) plus ionomycin (Io) and also in isolated murine CD4+T cells activated with anti-CD3 and anti-CD28 antibodies. Next, we examined the effect of tetradecanol on the transcriptional activity related to IL-2 production in T cells. Tetradecanol decreased PMA/Io-induced promoter activity of NF-κB in EL-4 T cells, but did not show any significant effects on the promoters of activator protein 1 (AP-1) and nuclear factor of activated T cells (NF-AT). Tetradecanol inhibited IκBα degradation and nuclear translocation of NF-κB subunit, p65 in PMA/Io-activated EL-4 T cells. These results suggest that tetradecanol might have immunosuppressive effects on T cell mediated disorders. Using a chronic allergic contact dermatitis model induced by repeated application of oxazolone, we showed that tetradecanol reduced ear thickness induced by oxazolone.
College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Gwangju 500-757, Republic of Korea.
Jung Up
JU
Park
College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Gwangju 500-757, Republic of Korea.
Bok Yun
BY
Kang
Department of Biomedical Science, Chonnam National University Medical School, Gwangju 500-757, Republic of Korea.
Hwa-Jeong
HJ
Lee
Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research (JINR), Jeollanamdo 529-851, Republic of Korea.
Sunoh
S
Kim
Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research (JINR), Jeollanamdo 529-851, Republic of Korea.
Donghyuck
D
Bae
Laboratory Animal Medicine and Brain Korea 21 PLUS Project, College of Veterinary Medicine, Chonnam National University, Gwangju 529-851, Republic of Korea.
Jong-Hwan
JH
Park
College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Gwangju 500-757, Republic of Korea. Electronic address: kimyr@chonnam.ac.kr.
Young Ran
YR
Kim
D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003877:Dermatitis, Contact / Q000150:complications / Q000188:drug therapy; D015536:Down-Regulation / Q000187:drug effects*; D005233:Fatty Alcohols / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D006367:HeLa Cells; D006801:Humans; D006967:Hypersensitivity / Q000150:complications; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D000072000:NF-KappaB Inhibitor alpha / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D059748:Proteolysis / Q000187:drug effects; D013601:T-Lymphocytes / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
D005233:Fatty Alcohols; D007376:Interleukin-2; D016328:NF-kappa B; D000072000:NF-KappaB Inhibitor alpha
Contact dermatitis; IL-2; NF-κB; T cell; Tetradecanol