Studies on expression pattern of toll-like receptor 5 (TLR5) in Edwardsiella tarda infected Pangasianodon hypophthalmus.
TLR5 is one of the important PRR (pathogen recognition receptors) and plays a fundamental role in pathogen recognition and activation of innate immune responses. It recognizes bacterial flagellin and stimulates the production of proinflammatory cytokines, through signalling via the adaptor protein MyD88. In this study, we characterized partial TLR5 (soluble form) gene from Pangasianodon hypophthalmus and analysed its expression profile upon challenge by Edwardsiella tarda. Bioinformatic analysis of gene sequence revealed a putative protein of 266 amino acids with four Leucine rich repeats. Quantitative expression analysis of TLR 5S showed its wide distribution in various organs and tissues. However, significant expression of TLR5S was observed in liver and spleen at 12 h (∼207.8 fold, p < 0.05). Significant upregulation was observed in kidney at 72 h.p.i. (50 folds, p < 0.05) indicating that the kidney provides longer protection almost till the activation of the adaptive immune system. This study enriches the knowledge of TLR5S in boosting the innate immunity against bacterial invasion in fish.
Division of Aquatic Environment and Health Management, ICAR-Central Institute of Fisheries Education, Mumbai, India.
Poojashree Kachigere
PK
Jayaramu
Division of Aquatic Environment and Health Management, ICAR-Central Institute of Fisheries Education, Mumbai, India.
Gayatri
G
Tripathi
Division of Fish Genetics and Biotechnology, ICAR-Central Institute of Fisheries Education, Mumbai, India.
A
A
Pavan Kumar
Division of Aquatic Environment and Health Management, ICAR-Central Institute of Fisheries Education, Mumbai, India.
Jeena
J
Keezhedath
Division of Fish Genetics and Biotechnology, ICAR-Central Institute of Fisheries Education, Mumbai, India.
Mujahid Khan
MK
Pathan
Division of Aquatic Environment and Health Management, ICAR-Central Institute of Fisheries Education, Mumbai, India. Electronic address: kpaniprasad@cife.edu.in.
Pani Prasad
PP
Kurcheti
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002397:Catfishes / Q000145:classification / Q000235:genetics / Q000276:immunology*; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D020609:Edwardsiella tarda / Q000276:immunology; D004756:Enterobacteriaceae Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D005393:Fish Diseases / Q000276:immunology*; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D007113:Immunity, Innate; D009928:Organ Specificity; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment / Q000662:veterinary; D051198:Toll-Like Receptor 5 / Q000737:chemistry / Q000235:genetics* / Q000276:immunology
D018076:DNA, Complementary; D029941:Fish Proteins; D012333:RNA, Messenger; D051198:Toll-Like Receptor 5
E. tarda; Innate immunity; Real time quantitative PCR; Toll like receptor; mRNA expression