Is there a cause-and-effect relationship between physicochemical properties and cell behavior of alginate-based hydrogel obtained after sterilization?
Alginate-based hydrogel scaffolds are widely used in the field of cartilage regeneration and repair. If the effect of autoclaving on the alginate powder is well known, it is not the same for the possible effects of the sterilization UV treatment on the properties of the hydrogel after polymerization. To select an effective sterilization treatment of alginate-based materials, one must find what are inter-relationship between the characteristics (chemical, physical and mechanical) of alginate-based hydrogel during sterilization, and what consequences have affected on cell behavior. In this study, we investigated the influence of UV sterilization treatments (UV-1 and UV-2: 25 and 50min, respectively) and autoclaving to obtain alginate (Alg)/hyaluronic acid (HA) hydrogel, as well as further evaluated the relationship between physicochemical properties and cell behavior of Alg/HA hydrogel after UVs and autoclaving. The physicochemical properties of this mixture at the powder or polymerized states were analyzed using ATR-FTIR, HPLC-SEC, rheological, indentation testing and sterility testing. The cell behaviors of hydrogels were evaluated by cell viability and proliferation, and chondrogenic differentiation. The effects of treatment parameters and their correlation with the others characteristics were determined statistically by Principal Component Analysis (PCA). In this study, we have shown that the cell behavior in alginate-based hydrogels was not only regulated by physicochemical properties (as molar mass or/and viscosity), but also associated with the controlling of sterilization time. It can provide a basis for choosing an effective method of sterilization, which can keep the mechanical or physical-chemical properties of Alg-based hydrogel scaffold and maintain its cytocompatibility and its ability to induce chondrogenesis from mesenchymal stem cells.
UMR 7365 CNRS, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Biopôle, Université de Lorraine, 54500 Vandoeuvre-lès-Nancy, France; Fédération de Recherche 3209, Bioingénierie Moléculaire Cellulaire et Thérapeutique, 54500 Vandoeuvre-lès-Nancy, France.
Hao
H
Yu
UMR 7365 CNRS, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Biopôle, Université de Lorraine, 54500 Vandoeuvre-lès-Nancy, France; Fédération de Recherche 3209, Bioingénierie Moléculaire Cellulaire et Thérapeutique, 54500 Vandoeuvre-lès-Nancy, France.
Ghislaine
G
Cauchois
UMR 7563 CNRS, Group of Biomechanics and Bioengineering, Université de Lorraine, 54500 Vandoeuvre-lès-Nancy, France.
Jean-François
JF
Schmitt
UMR 7563 CNRS, Group of Biomechanics and Bioengineering, Université de Lorraine, 54500 Vandoeuvre-lès-Nancy, France.
Nicolas
N
Louvet
UMR 7375, Laboratoire de Chime-Physique Macromoléculaire, ENSIC, Université de Lorraine, 54001 Nancy Cedex, France.
Jean-Luc
JL
Six
Department of Biomedical Engineering, School of Basic Medical Science, Wuhan University, Wuhan 430071, China.
Yun
Y
Chen
UMR 7563 CNRS, Group of Biomechanics and Bioengineering, Université de Lorraine, 54500 Vandoeuvre-lès-Nancy, France.
Rachid
R
Rahouadj
UMR 7365 CNRS, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Biopôle, Université de Lorraine, 54500 Vandoeuvre-lès-Nancy, France; Fédération de Recherche 3209, Bioingénierie Moléculaire Cellulaire et Thérapeutique, 54500 Vandoeuvre-lès-Nancy, France. Electronic address: celine.huselstein@univ-lorraine.fr.
Céline
C
Huselstein
D000464:Alginates / Q000737:chemistry*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D020219:Chondrogenesis; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D013242:Sterilization
D000464:Alginates; D006603:Hexuronic Acids; D020100:Hydrogels; D020723:Glucuronic Acid
Alginate; Autoclave; Hydrogel; Sterilization; UV