Nanosilicalites as Support for β-Glucosidases Covalent Immobilization.
Many different materials have been tested for β-glucosidases immobilization. Such materials, however, often show a poor activity related to a low surface area of the support or even enzyme hindrance caused by entrapment inside porous matrix. In this context, the use of nanosized zeolites as enzymes support is quite new and may be an interesting alternative. The present work evaluates the immobilization of β-glucosidases in nanosized silicalites by covalent coupling. The new biocatalyst was able to convert 100% of cellobiose into glucose in 18 h at 50 °C and pH 5, retaining 85% of its activity after five cycles of reuse. A detailed investigation of the published literature indicates that, apparently, this is the first work concerning the immobilization of β-glucosidases on nanosized zeolites ever reported.
Postgraduate Program in Technology of Chemical and Biochemical Processes, School of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, 21941-909, Brazil.
Y
Y
Carvalho
Postgraduate Program in Technology of Chemical and Biochemical Processes, School of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, 21941-909, Brazil.
J M A R
JMAR
Almeida
Postgraduate Program in Technology of Chemical and Biochemical Processes, School of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, 21941-909, Brazil.
P N
PN
Romano
MicroBioRefinery Facility, Stephenson's Institute for Renewable Energy, Department of Chemistry, University of Liverpool, Liverpool, L69 7ZD, UK.
K
K
Farrance
MicroBioRefinery Facility, Stephenson's Institute for Renewable Energy, Department of Chemistry, University of Liverpool, Liverpool, L69 7ZD, UK.
P
P
Demma Carà
Postgraduate Program in Technology of Chemical and Biochemical Processes, School of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, 21941-909, Brazil. nei@eq.ufrj.br.
N
N
Pereira
MicroBioRefinery Facility, Stephenson's Institute for Renewable Energy, Department of Chemistry, University of Liverpool, Liverpool, L69 7ZD, UK.
J A
JA
Lopez-Sanchez
Postgraduate Program in Technology of Chemical and Biochemical Processes, School of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, 21941-909, Brazil.
E F
EF
Sousa-Aguiar
D055162:Biocatalysis; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D006868:Hydrolysis; D049329:Nanostructures / Q000737:chemistry*; D017640:Silicates / Q000737:chemistry*; D013499:Surface Properties; D001617:beta-Glucosidase / Q000737:chemistry* / Q000378:metabolism*
D004800:Enzymes, Immobilized; D017640:Silicates; D001617:beta-Glucosidase
Cellobiose; Enzymes; Immobilization; Silicalite; Zeolites; β-glucosidase