Preparation of Protein Extracts from Schizosaccharomyces pombe Using Trichloroacetic Acid Precipitation.
Schizosaccharomyces pombe is an attractive model organism with which to study core principles of conserved molecular cell biology processes. The ability to monitor protein behavior following separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) underpins much of this activity. Here we describe a robust protocol for the preparation of protein samples for analysis by SDS-PAGE.
CRUK Cell Division Group, Cancer Research UK Manchester Institute, University of Manchester, Manchester M20 4BX, United Kingdom Agnes.Grallert@cruk.manchester.ac.uk Iain.Hagan@cruk.manchester.ac.uk.
Agnes
A
Grallert
CRUK Cell Division Group, Cancer Research UK Manchester Institute, University of Manchester, Manchester M20 4BX, United Kingdom Agnes.Grallert@cruk.manchester.ac.uk Iain.Hagan@cruk.manchester.ac.uk.
Iain M
IM
Hagan
D002424:Caustics / Q000378:metabolism; D011232:Chemical Precipitation; D005656:Fungal Proteins / Q000302:isolation & purification*; D012568:Schizosaccharomyces / Q000737:chemistry*; D014238:Trichloroacetic Acid / Q000378:metabolism*
D002424:Caustics; D005656:Fungal Proteins; D014238:Trichloroacetic Acid