Therapeutic Prospective of Infused Allogenic Cultured Mesenchymal Stem Cells in Traumatic Brain Injury Mice: A Longitudinal Proton Magnetic Resonance Spectroscopy Assessment.
Improved therapeutic assessment of experimental traumatic brain injury (TBI), using mesenchymal stem cells (MSCs), would immensely benefit its therapeutic management. Neurometabolite patterns at injury site, measured with proton magnetic resonance spectroscopy (1H-MRS) after MSCs transplantation, may serve as a bio-indicator of the recovery mechanism. This study used in vivo magnetic resonance imaging and 1H-MRS to evaluate the therapeutic prospects of implanted MSCs at injury site in experimental mice longitudinally up to 21 days. Negative tissue contrast and cytotoxic edema formation were observed in susceptibility-based contrast (T2*) and an apparent diffusion coefficient map, respectively. Lesion site showed decreased N-acetylaspartate, total choline, myo-inositol, total creatine, glutamate-glutamine complex, and taurine neurometabolic concentrations by 1H-MRS investigation. There was a considerable decrease in locomotor activity, depression index, and cognitive index after TBI. It may, therefore, be inferred that MSC transplantation prompted recovery by decreasing negative signals and edema, restoring metabolites to baseline concentrations, and enhancing behavioral activity. Overall findings support the potential of MSC transplantation for the enhancement of endogenous neuroprotective responses, which may provide future clinical applications for translating laboratory research into therapeutic clinical advances. Stem Cells Translational Medicine 2017;6:316-329.
NMR Research Centre, Institute of Nuclear Medicine and Allied Sciences, Defense Research and Development Organisation, Timarpur, Delhi, India.|Division of Stem Cell and Gene Therapy Research, Institute of Nuclear Medicine and Allied Sciences, Defence Research and Development Organisation, Timarpur, Delhi, India.
Sushanta Kumar
SK
Mishra
NMR Research Centre, Institute of Nuclear Medicine and Allied Sciences, Defense Research and Development Organisation, Timarpur, Delhi, India.
Poonam
P
Rana
NMR Research Centre, Institute of Nuclear Medicine and Allied Sciences, Defense Research and Development Organisation, Timarpur, Delhi, India.
Subash
S
Khushu
Division of Stem Cell and Gene Therapy Research, Institute of Nuclear Medicine and Allied Sciences, Defence Research and Development Organisation, Timarpur, Delhi, India.
Gurudutta
G
Gangenahalli
D000818:Animals; D001522:Behavior, Animal; D001921:Brain / Q000473:pathology; D000070642:Brain Injuries, Traumatic / Q000473:pathology / Q000628:therapy*; D002454:Cell Differentiation; D002478:Cells, Cultured; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008807:Mice, Inbred BALB C; D010641:Phenotype; D066244:Proton Magnetic Resonance Spectroscopy; D014184:Transplantation, Homologous; D014867:Water
D014867:Water
Magnetic resonance imaging; Mesenchymal stem cells; Proton magnetic resonance spectroscopy; Stem cell therapy; Traumatic brain injury