VcBBX, VcMYB21, and VcR2R3MYB Transcription Factors Are Involved in UV-B-Induced Anthocyanin Biosynthesis in the Peel of Harvested Blueberry Fruit.
This study was carried out to better understand the mechanism responsible for increasing the anthocyanins in blueberries after UV-B radiation at 6.0 kJ m-2for 20 min. UV-B induced upregulation of genes involved in anthocyanin biosynthesis in blueberry fruit compared to a nontreated control. Phenylalanine ammonia lyase, chalcone synthase, and flavanone 3'-hydroxylase, which are enzymes that function upstream of anthocyanin biosynthesis, were significantly expressed by UV-B. Expression levels of VcBBX, VcMYB21, and VcR2R3MYB transcription factors (TFs) were upregulated by UV-B in the same manner as the anthocyanin biosynthesis genes. The significant increase in the expression of TFs occurred immediately after UV-B treatment and was then maximized within 3 h. In accordance with these changes, individual anthocyanin contents in the fruits treated with UV-B significantly increased within 6 h and were 2-3-fold higher than the control. Our results indicated that UV-B radiation stimulates an increase in anthocyanin biosynthesis, which could be upregulated by the TFs studied.
Department of Plant Science, Research Institute of Agriculture and Life Sciences, Seoul National University , Seoul 151-921, Korea.
Chau T T
CT
Nguyen
Department of Plant Science, Research Institute of Agriculture and Life Sciences, Seoul National University , Seoul 151-921, Korea.
Sooyeon
S
Lim
Department of Plant Science, Research Institute of Agriculture and Life Sciences, Seoul National University , Seoul 151-921, Korea.
Jeong Gu
JG
Lee
Department of Plant Science, Research Institute of Agriculture and Life Sciences, Seoul National University , Seoul 151-921, Korea.
Eun Jin
http://orcid.org/0000-0002-5565-1930
EJ
Lee
D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D000872:Anthocyanins / Q000096:biosynthesis*; D029798:Blueberry Plants / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D005638:Fruit / Q000201:enzymology / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects*; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014466:Ultraviolet Rays
D000872:Anthocyanins; D010940:Plant Proteins; D014157:Transcription Factors; D006899:Mixed Function Oxygenases; C048797:flavanone 3-dioxygenase; D000217:Acyltransferases; C020141:flavanone synthetase; D010650:Phenylalanine Ammonia-Lyase
UV radiation; Vaccinium corymbosum; anthocyanins; pigment synthesis; postharvest; transcription factors; treatment