Exploiting Uptake of Nanoparticles by Phagocytes for Cancer Treatment.
Many cancers including ovarian, pancreatic, colon, liver, and stomach cancers are largely confined to the peritoneal cavity. Peritoneal tumors are directly accessible by intraperitoneal injections. Previously we demonstrated that intraperitoneal injection of nanoparticles and subsequent ingestion by tumor-associated phagocytes can be used to either directly impact tumors or stimulate antitumor immune responses. Here we outline methods to specifically utilize iron oxide nanoparticles with the ID8-Defb29/Vegf-A murine ovarian cancer model and discuss the tendency of phagocytes to ingest nanoparticles and the potential of phagocytes to carry nanoparticles to tumors resulting in direct killing of tumor cells or stimulate antitumor immune responses in peritoneal cancers. This basic approach can be modified as needed for different types of tumors and nanoparticles.
Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, NH, 03756, USA.
Mee Rie
MR
Sheen
Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, NH, 03756, USA. steve.fiering@dartmouth.edu.|Department of Genetics, Geisel School of Medicine at Dartmouth, Hanover, NH, 03755, USA. steve.fiering@dartmouth.edu.|Norris Cotton Cancer Center, Dartmouth Hitchcock Medical Center, 1 Medical Center Drive, 622 Rubin DHMC, Lebanon, NH, 03756, USA. steve.fiering@dartmouth.edu.
Steven
S
Fiering
D000818:Animals; D015415:Biomarkers; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods; D004195:Disease Models, Animal; D005290:Ferric Compounds / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry; D009369:Neoplasms / Q000276:immunology / Q000628:therapy; D010586:Phagocytes / Q000276:immunology* / Q000378:metabolism*; D010587:Phagocytosis; D013213:Starch / Q000737:chemistry; D023041:Xenograft Model Antitumor Assays
D015415:Biomarkers; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; C001215:ferrite; D013213:Starch
Antitumor immune responses; ID8-Defb29/Vegf-A; Intraperitoneal injection; Iron oxide nanoparticles; Nanoparticles; Ovarian cancer; Peritoneal cavity; Peritoneal tumor; Tumor-associated phagocytes