Actinomadura Species: Laboratory Maintenance and Ribosome Engineering.
Actinomadura spp. are aerobic, Gram-positive, catalase-positive, non-acid fast, non-motile actinomycetes. Some species of Actinomadura are associated with opportunistic infections in humans. However, many bioactive compounds with pharmaceutical applications can be isolated from various Actinomadura spp. This unit includes general protocols for the laboratory maintenance of Actinomadura spp., including growth in liquid medium, growth on solid agar, long-term storage, and generation of a higher producing strain by ribosome engineering. Actinomadura hibisca P157-2 is used as a prototype for explaining the considerations for efficient laboratory maintenance of Actinomadura spp. © 2017 by John Wiley & Sons, Inc.
Department of Life Science and Biochemical Engineering, Sun Moon University, Asan-si, Republic of Korea.
Dipesh
D
Dhakal
Department of Life Science and Biochemical Engineering, Sun Moon University, Asan-si, Republic of Korea.
Nguyen Thanh
NT
Chung
Department of Life Science and Biochemical Engineering, Sun Moon University, Asan-si, Republic of Korea.
Vijay
V
Rayamajhi
Department of Life Science and Biochemical Engineering, Sun Moon University, Asan-si, Republic of Korea.|Department of BT-Convergent Pharmaceutical Engineering, Sun Moon University, Asan-si, Republic of Korea.
Jae Kyung
JK
Sohng
D039903:Actinobacteria / Q000235:genetics* / Q000254:growth & development*; D001431:Bacteriological Techniques / Q000379:methods*; D003470:Culture Media / Q000737:chemistry; D005827:Genetics, Microbial / Q000379:methods*; D060847:Metabolic Engineering / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D011309:Preservation, Biological / Q000379:methods; D012270:Ribosomes / Q000235:genetics*
D003470:Culture Media
Actinomadura spp; growth medium; ribosome engineering; storage