In vitrostudy of biological activity of four strains ofBurkholderia gladiolipv.agaricicolaand identification of their bioactive metabolites using GC-MS.
This research was carried out to studyin vitroantibacterial activity of 4 strains ofBurkholderia gladiolipv.agaricicola(Bga) against G+veBacillus megateriumand G-veEscherichia coli, haemolytic activity against the cell membrane of erythrocytes, the production of extracellular hydrolytic enzymes and finally, the pathogenicity againstAgaricus bisporusflesh blocks. Chemical structure of bioactive substances of the most bioactive strain (ICMP 11096) was established using gas chromatography-mass spectrometry (GC-MS). All the studiedBgastrains inhibited the growth of the two tested bacteria although some growing substrates negatively influenced the antimicrobial substance production. The sameBgastrains showed highly haemolytic activity and were able to produce 3 hydrolytic enzymes, i.e. chitinase, glucanase and protease. In pathogenicity assays, the consideredBgastrains resulted virulent forA.bisporus. The GC-MS for compounds fromBgaICMP 11096 were compatible with the structure of two bioactive fatty acids identified as methyl stearate and ethanol 2-butoxy phosphate with mass spectrum m/e 298 and 398, respectively.
School of Agricultural, Forestry, Food and Environmental Sciences, University of Basilicata, Viale Ateneo Lucano, 85100 Potenza, Italy.
Hazem S
HS
Elshafie
Department of Science, University of Basilicata, Viale dell'Ateneo Lucano, 85100 Potenza, Italy.
Rocco
R
Racioppi
Department of Science, University of Basilicata, Viale dell'Ateneo Lucano, 85100 Potenza, Italy.
Sabino A
SA
Bufo
School of Agricultural, Forestry, Food and Environmental Sciences, University of Basilicata, Viale Ateneo Lucano, 85100 Potenza, Italy.
Ippolito
I
Camele
Agaricus bisporus; Antimicrobial activity; Erythrocytes; Extracellular hydrolytic enzymes; GC–MS