Serum proteomic-based analysis identifying autoantibodies against PRDX2 and PRDX3 as potential diagnostic biomarkers in nasopharyngeal carcinoma.
BACKGROUND
Nasopharyngeal carcinoma (NPC) is a major head and neck cancer with high occurrence in Southeast Asia and southern China. We aimed to identify autoantibodies that may contribute to early detection of NPC.


METHODS
We used serological proteome analysis to identify candidate autoantibodies against tumor-associated antigens. Levels of autoantibodies and Epstein-Barr virus capsid antigen-IgA (VCA-IgA) were measured by ELISA in 129 patients with NPC and 100 normal controls. We employed receiver operating characteristics to calculate diagnostic accuracy.


RESULTS
Sera from patients with NPC yielded multiple spots, two of which were identified as PRDX2 and PRDX3. Levels of serum autoantibodies against PRDX2 and PRDX3 were significantly higher for patients with NPC than for normal controls (P< 0.01), respectively. Combined detection of autoantibodies against PRDX2 and PRDX3 and VCA-IgA provided a high diagnostic accuracy in NPC (an area under the curve (AUC) of 0.811 (95% CI 0.753-0.869), 66.7% sensitivity, and 95.0% specificity). This combination maintained diagnostic performance for early NPC with AUC value of 0.754 (95% CI 0.652-0.857), 50.0% sensitivity, and 95.0% specificity.


CONCLUSIONS
This study reports autoantibodies against PRDX2 and PRDX3 identified by a proteomic approach in sera from NPC patients. Our findings suggest that autoantibodies against PRDX2 and PRDX3 may serve as supplementary biomarkers to VCA-IgA for the screening and diagnosis of NPC.
Department of Clinical Laboratory Medicine, The Cancer Hospital of Shantou University Medical College, No. 7, Raoping Road, Shantou, 515041 Guangdong China.|Department of Orthopaedics, The Nanao People's Hospital, Shantou, 515999 China.
Lie-Hao
LH
Lin
Department of Clinical Laboratory Medicine, The Cancer Hospital of Shantou University Medical College, No. 7, Raoping Road, Shantou, 515041 Guangdong China.|The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, 515041 China.
Yi-Wei
YW
Xu
Department of Radiation Oncology, The Cancer Hospital of Shantou University Medical College, Shantou, 515041 China.
Li-Sheng
LS
Huang
Department of Oncological Laboratory Research, The Cancer Hospital of Shantou University Medical College, Shantou, 515041 China.
Chao-Qun
CQ
Hong
Department of Radiation Oncology, The Cancer Hospital of Shantou University Medical College, Shantou, 515041 China.
Tian-Tian
TT
Zhai
The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, 515041 China.|Institute of Oncological Pathology, Shantou University Medical College, Shantou, 515041 China.
Lian-Di
LD
Liao
Department of Orthopaedics, The Nanao People's Hospital, Shantou, 515999 China.
Wen-Jie
WJ
Lin
The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, 515041 China.|Institute of Oncological Pathology, Shantou University Medical College, Shantou, 515041 China.
Li-Yan
LY
Xu
Tianjin Key Laboratory of Medical Epigenetics, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, 300070 China.
Kai
K
Zhang
The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, 515041 China.|Department of Biochemistry and Molecular Biology, Shantou University Medical College, Shantou, 515041 China.
En-Min
EM
Li
Department of Clinical Laboratory Medicine, The Cancer Hospital of Shantou University Medical College, No. 7, Raoping Road, Shantou, 515041 Guangdong China.|The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, 515041 China.
Yu-Hui
YH
Peng
Autoantibody; Early diagnosis; Nasopharyngeal carcinoma; Peroxiredoxin; Serological proteome analysis