Relationship of oxidative stress with elevated level of DNA damage and homocysteine in cardiovascular disease patients.
Amounts of DNA damage and homocysteine (Hcy) in heart patients blood may have strong function in the causation of cardiovascular disease (CVD). The main objective of this work was to know experimentally the role of total oxidants (produced by Reactive Oxygen species (ROS), clinical biochemical indices, their oxidized products and total antioxidant status (TAS) among such patients to find the association of homocysteine, total oxidation status (TOS) and oxidative DNA damage with other clinical parameters in sixty positive CVD patients compared with those of 60 normal subjects. As compared to healthy individuals, CVD patients had significantly higher concentrations of homocysteine (p<0.0001), total oxidants stress (TOS) (p<0.0001), serum total lipids (p<0.04), malondialdehyde (MDA) (p<0.001), high density lipoprotein-cholesterol (HDL-C) (p<0.0001), and low density lipoprotein cholesterol (LDL-C) (p<0.01), than those of healthy individuals. Plasma Hcy content, TOS and amount of DNA were positively and significantly associated with cholesterol, triglycerides, systolic blood pressure, urea, and albumin (p values<0.01). TOS, Hcy and oxidative DNA damage were negatively correlated with HDL-c, TAS and proteins. It is suggested that these parameters have pivotal role in diagnostic process of determining severity in CAD patients. Oxidized products of macromolecules in blood of CVD patients impart major functions in causing CVD disease.
Department of Bioinformatics and Biotechnology, Government College University (GCUF), Faisalabad, Pakistan.
Muhammad
M
Qasim
Department of Applied Chemistry and Biochemistry, Government College University (GCUF), Faisalabad, Pakistan.
Shazia Anwer
SA
Bukhari
Department of Bioinformatics and Biotechnology, Government College University (GCUF), Faisalabad, Pakistan.
Madiha Javeed
MJ
Ghani
Department of Bioinformatics and Biotechnology, Government College University (GCUF), Faisalabad, Pakistan.
Muhammad Shareef
MS
Masoud
Department of Bioinformatics and Biotechnology, Government College University (GCUF), Faisalabad, Pakistan.
Tayyabah
T
Huma
Department of Biochemistry, College of Veterinary and Animal Sciences, Jhang Campus, Jhang, Pakistan.
Muhammad
M
Arshad
Department of Bioinformatics and Biotechnology, Government College University (GCUF), Faisalabad, Pakistan.
Asma
A
Haque
College of Pharmacy, Government College University (GCUF), Faisalabad, Pakistan.
Zubair
Z
Ibrahim
Department of Applied Chemistry and Biochemistry, Government College University (GCUF), Faisalabad, Pakistan.
Sadia
S
Javed
Department of Bioinformatics and Biotechnology, Government College University (GCUF), Faisalabad, Pakistan.
Muhammad Ibrahim
MI
Rajoka
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000368:Aged; D015415:Biomarkers / Q000097:blood; D002318:Cardiovascular Diseases / Q000097:blood* / Q000175:diagnosis / Q000235:genetics; D016022:Case-Control Studies; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood; D005260:Female; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D008055:Lipids / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D015854:Up-Regulation
D015415:Biomarkers; D008055:Lipids; D017392:Thiobarbituric Acid Reactive Substances; D006710:Homocysteine; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine