title: Inactivation of viruses by pasteurization at 60 °C for 10 h with and without 40% glucose as stabilizer during a new manufacturing process of α2-Macroglobulin from Cohn Fraction IV.
abstract: Pasteurization is regularly used to inactivate viruses for the safety of plasma derivatives. Influence of pasteurization at 60 °C for 10 h on α2-Macroglobulin activity and virus inactivation were studied. With 40% sugar as stabilizers more than 70% α2-Macroglobulin activity was reserved after pasteurization compared with 20% in control. Glucose presented a better activity protection effect than sucrose and maltose. By pasteurization without stabilizer the virus titers of pseudorabies virus, Sindbis virus, porcine parvovirus and encephalomyocarditis virus were reduced more than 5.88 log10, 7.50 log10, 4.88 log10, and 5.63 log10respectively within 2 h. By pasteurization with 40% glucose vesicular stomatitis virus was inactivated more than 5.88 log10within 1 h. Only 2.71 log10reduction was achieved for encephalomyocarditis virus after 10 h. 40% glucose protected α2-M activity and viruses simultaneously from pasteurization. Other viral inactivation methods need to be incorporated to ensure viral safety of this manufacturing process of α2-Macroglobulin.
authors.affiliation: Beijing Key Laboratory of Blood Safety and Supply Technologies, Beijing Institute of Transfusion Medicine, Beijing, 100850, China.
authors.forename: Chaoji
authors.initials: C
authors.lastname: Huangfu
authors.affiliation: Beijing Key Laboratory of Blood Safety and Supply Technologies, Beijing Institute of Transfusion Medicine, Beijing, 100850, China.
authors.forename: Yuyuan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Beijing Key Laboratory of Blood Safety and Supply Technologies, Beijing Institute of Transfusion Medicine, Beijing, 100850, China.
authors.forename: Junting
authors.initials: J
authors.lastname: Jia
authors.affiliation: Beijing Key Laboratory of Blood Safety and Supply Technologies, Beijing Institute of Transfusion Medicine, Beijing, 100850, China.
authors.forename: Maomin
authors.initials: M
authors.lastname: Lv
authors.affiliation: Beijing Key Laboratory of Blood Safety and Supply Technologies, Beijing Institute of Transfusion Medicine, Beijing, 100850, China.
authors.forename: Fengxuan
authors.initials: F
authors.lastname: Zhu
authors.affiliation: Hualan Biological Engineering Inc, Xinxiang, 453003, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Ma
authors.affiliation: Beijing Key Laboratory of Blood Safety and Supply Technologies, Beijing Institute of Transfusion Medicine, Beijing, 100850, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Beijing Key Laboratory of Blood Safety and Supply Technologies, Beijing Institute of Transfusion Medicine, Beijing, 100850, China. Electronic address: zhangjg0707@aliyun.com.
authors.forename: Jingang
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D002460:Cell Line; D004686:Encephalomyocarditis virus / Q000502:physiology; D005947:Glucose / Q000494:pharmacology*; D011558:Herpesvirus 1, Suid / Q000502:physiology; D006358:Hot Temperature; D006801:Humans; D028323:Parvovirus, Porcine / Q000502:physiology; D061352:Pasteurization / Q000379:methods*; D015203:Reproducibility of Results; D012845:Sindbis Virus / Q000502:physiology; D013552:Swine; D013997:Time Factors; D014709:Vero Cells; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects / Q000502:physiology; D038301:Virus Inactivation / Q000187:drug effects*; D000511:alpha-Macroglobulins / Q000378:metabolism*
chemical_list: D001798:Blood Proteins; C032309:Cohn fraction IV; D000511:alpha-Macroglobulins; D005947:Glucose
keywords: Cohn Fraction IV; Pasteurization; Virus inactivation; α2-Macroglobulin