title: Phylogenomic relationship of feijoa (Acca sellowiana (O.Berg) Burret) with other Myrtaceae based on complete chloroplast genome sequences.
abstract: Given their distribution, importance, and richness, Myrtaceae species comprise a model system for studying the evolution of tropical plant diversity. In addition, chloroplast (cp) genome sequencing is an efficient tool for phylogenetic relationship studies. Feijoa [Acca sellowiana (O. Berg) Burret; CN: pineapple-guava] is a Myrtaceae species that occurs naturally in southern Brazil and northern Uruguay. Feijoa is known for its exquisite perfume and flavorful fruits, pharmacological properties, ornamental value and increasing economic relevance. In the present work, we reported the complete cp genome of feijoa. The feijoa cp genome is a circular molecule of 159,370 bp with a quadripartite structure containing two single copy regions, a Large Single Copy region (LSC 88,028 bp) and a Small Single Copy region (SSC 18,598 bp) separated by Inverted Repeat regions (IRs 26,372 bp). The genome structure, gene order, GC content and codon usage are similar to those of typical angiosperm cp genomes. When compared to other cp genome sequences of Myrtaceae, feijoa showed closest relationship with pitanga (Eugenia uniflora L.). Furthermore, a comparison of pitanga synonymous (Ks) and nonsynonymous (Ka) substitution rates revealed extremely low values. Maximum Likelihood and Bayesian Inference analyses produced phylogenomic trees identical in topology. These trees supported monophyly of three Myrtoideae clades.
authors.affiliation: Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Programa de Pós-graduação em Recursos Genéticos Vegetais, Universidade Federal de Santa Catarina, Rodovia Admar Gonzaga, 1346, 88040-900, Florianópolis, Santa Catarina, Brazil.
authors.forename: Lilian de Oliveira
authors.initials: LO
authors.lastname: Machado
authors.affiliation: Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Programa de Pós-graduação em Recursos Genéticos Vegetais, Universidade Federal de Santa Catarina, Rodovia Admar Gonzaga, 1346, 88040-900, Florianópolis, Santa Catarina, Brazil.
authors.forename: Leila do Nascimento
authors.initials: LD
authors.lastname: Vieira
authors.affiliation: Núcleo de Genômica e Ecologia Molecular, Programa de Pós-graduação em Ciências Biológicas, Universidade Federal do Pampa, Av. Antônio Trilha 1847, 97300-000, São Clemente, São Gabriel, Rio Grande Do Sul, Brazil.
authors.forename: Valdir Marcos
authors.initials: VM
authors.lastname: Stefenon
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Núcleo de Fixação Biológica de Nitrogênio, Universidade Federal do Paraná, Rua Coronel Francisco H. dos Santos s/n, 81531-980, Curitiba, Paraná, Brazil.
authors.forename: Fábio de
authors.initials: F
authors.lastname: Oliveira Pedrosa
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Núcleo de Fixação Biológica de Nitrogênio, Universidade Federal do Paraná, Rua Coronel Francisco H. dos Santos s/n, 81531-980, Curitiba, Paraná, Brazil.
authors.forename: Emanuel Maltempi de
authors.initials: EM
authors.lastname: Souza
authors.affiliation: Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Programa de Pós-graduação em Recursos Genéticos Vegetais, Universidade Federal de Santa Catarina, Rodovia Admar Gonzaga, 1346, 88040-900, Florianópolis, Santa Catarina, Brazil.
authors.forename: Miguel Pedro
authors.initials: MP
authors.lastname: Guerra
authors.affiliation: Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Programa de Pós-graduação em Recursos Genéticos Vegetais, Universidade Federal de Santa Catarina, Rodovia Admar Gonzaga, 1346, 88040-900, Florianópolis, Santa Catarina, Brazil. rubens.nodari@ufsc.br.
authors.forename: Rubens Onofre
authors.initials: RO
authors.lastname: Nodari
mesh_terms: D001482:Base Composition / Q000235:genetics; D001499:Bayes Theorem; D001938:Brazil; D003062:Codon / Q000235:genetics; D004270:DNA, Circular / Q000737:chemistry / Q000235:genetics*; D031646:Feijoa / Q000145:classification / Q000235:genetics*; D023061:Gene Order; D061125:Genes, Chloroplast / Q000235:genetics; D054628:Genome, Chloroplast / Q000235:genetics*; D008390:Markov Chains; D009010:Monte Carlo Method; D009154:Mutation; D027822:Myrtaceae / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
chemical_list: D003062:Codon; D004270:DNA, Circular
keywords: Feijoa; Myrtaceae; Next-generation sequencing; Phylogenomics; Plastid