title: Curcumin Attenuates N-Nitrosodiethylamine-Induced Liver Injury in Mice by Utilizing the Method of Metabonomics.
abstract: N-Nitrosodiethylamine (DEN) exists as a food additive in cheddar cheese, processed meats, beer, water, and so forth. It is a potent hepatocarcinogen in animals and humans. Curcumin as a natural dietary compound decreased DEN-induced hepatocarcinogenesis in this research. According to the histopathological examination of liver tissues and biomarker detection in serum and livers, it was demonstrated that curcumin attenuated DEN-induced hepatocarcinogenesis through parts of regulating the oxidant stress enzymes (T-SOD and CAT), liver function (ALT and AST) and LDHA, AFP level, and COX-2/PGE2 pathway. Furthermore, curcumin attenuated metabolic disorders via increasing concentration of glucose and fructose, and decreasing levels of glycine and proline, and mRNA expression of GLUT1, PKM and FASN. Docking study indicated that curcumin presented strong affinity with key metabolism enzymes such as GLUT1, PKM, FASN and LDHA. There were a number of amino acid residues involved in curcumin-targeting enzymes of hydrogen bonds and hydrophobic interactions. All in all, curcumin exhibited a potent liver protective agent inhibiting chemically induced liver injury through suppressing liver cellular metabolism in the prospective application.
authors.affiliation: Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industry Microbiology, National and Local United Engineering Lab of Metabolic Control Fermentation Technology, College of Biotechnology, Tianjin University of Science & Technology , Tianjin 300457, China.
authors.forename: Peiyu
authors.initials: P
authors.lastname: Qiu
authors.affiliation: Tianjin University of Traditional Chinese Medicine , Tianjin 300193, China.
authors.forename: Jiachen
authors.initials: J
authors.lastname: Sun
authors.affiliation: Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industry Microbiology, National and Local United Engineering Lab of Metabolic Control Fermentation Technology, College of Biotechnology, Tianjin University of Science & Technology , Tianjin 300457, China.
authors.forename: Shuli
authors.identifier: http://orcid.org/0000-0002-0864-0622
authors.initials: S
authors.lastname: Man
authors.affiliation: Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industry Microbiology, National and Local United Engineering Lab of Metabolic Control Fermentation Technology, College of Biotechnology, Tianjin University of Science & Technology , Tianjin 300457, China.
authors.forename: He
authors.initials: H
authors.lastname: Yang
authors.affiliation: Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industry Microbiology, National and Local United Engineering Lab of Metabolic Control Fermentation Technology, College of Biotechnology, Tianjin University of Science & Technology , Tianjin 300457, China.
authors.forename: Long
authors.initials: L
authors.lastname: Ma
authors.affiliation: Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industry Microbiology, National and Local United Engineering Lab of Metabolic Control Fermentation Technology, College of Biotechnology, Tianjin University of Science & Technology , Tianjin 300457, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Yu
authors.affiliation: Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University , Tianjin 300072, China.
authors.forename: Wenyuan
authors.identifier: http://orcid.org/0000-0001-8475-6988
authors.initials: W
authors.lastname: Gao
mesh_terms: D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000201:enzymology / Q000209:etiology / Q000235:genetics; D003474:Curcumin / Q000008:administration & dosage*; D004052:Diethylnitrosamine / Q000633:toxicity*; D006801:Humans; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D055432:Metabolomics; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects
chemical_list: D004052:Diethylnitrosamine; D003474:Curcumin
keywords: curcumin; diethylnitrosamine; liver cancer; metabolomics; structural simulation