title: Paper-based microfluidic devices for electrochemical immunofiltration analysis of human chorionic gonadotropin.
abstract: An electrochemical immunofiltration analysis was introduced into microfluidic paper-based analytical devices (μPADs) for the first time, which was based on photolithography and screen-printing technology. The hydrophilic test zones of the aldehyde-functionalized screen-printed electrodes (SPEs) were biofunctionalized with capture antibodies (Ab1). A sensitive immune detection method was developed by using primary signal antibody functionalized gold nanoparticles (GNPs/Ab2) and alkaline phosphatase conjugated secondary antibody (ALP-IgG). Differential pulse voltammetry (DPV) was performed to detect the electrochemical response. The microfluidic paper-based electrochemical immunosensor (μ-PEI) was optimized and characterized for the detection of human chorionic gonadotropin (HCG), a model analyte, in a linear range from 1.0mIUmL-1to 100.0 IU mL-1with a detection limit of 0.36mIUmL-1. Additionally, the proposed μ-PEI was used to test HCG in real human serum and obtained satisfactory results. The disposable, efficient, sensitive and low-cost μ-PEI has exhibited great potential for the development of point-of-care testing (POCT) devices that can be applicated in healthcare monitoring.
authors.affiliation: Department of Pharmacy Engineering, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
authors.forename: Liangli
authors.initials: L
authors.lastname: Cao
authors.affiliation: School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin 541014, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Fang
authors.affiliation: School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin 541014, China.
authors.forename: Ruosheng
authors.initials: R
authors.lastname: Zeng
authors.affiliation: Department of Pharmacy Engineering, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
authors.forename: Xiongjie
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Pharmacy Engineering, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China. Electronic address: jiangyr@mail.csu.edu.cn.
authors.forename: Yuren
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin 541014, China. Electronic address: chenzhcheng@163.com.
authors.forename: Zhencheng
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry; D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D006063:Chorionic Gonadotropin / Q000097:blood*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D005374:Filtration / Q000295:instrumentation; D006046:Gold / Q000737:chemistry; D006801:Humans; D018796:Immunoconjugates / Q000737:chemistry; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D056656:Lab-On-A-Chip Devices; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D010209:Paper; D000067716:Point-of-Care Testing
chemical_list: D055910:Antibodies, Immobilized; D006063:Chorionic Gonadotropin; D018796:Immunoconjugates; D006046:Gold; D000469:Alkaline Phosphatase
keywords: Electrochemical immunofiltration; Human chorionic gonadotropin; Paper-based microfluidic devices; Photolithography; Point-of-care testing