title: Trueperella pyogenes isolated from dairy cows with endometritis in Inner Mongolia, China: Tetracycline susceptibility and tetracycline-resistance gene distribution.
abstract: Trueperella pyogenes plays a crucial role in endometritis pathogenesis and is also associated with many infections, including metritis, mastitis, arthritis and liver abscessation, in many domestic animals. In this study, we investigated the prevalence of tetracycline resistance in T. pyogenes isolated from dairy cows with endometritis in Inner Mongolia, China, and we assessed tetracycline-resistance gene distribution among the isolates. Our results indicated that 68.7% and 62.5% of the isolates were resistant to tetracycline and doxycycline, respectively, and the rate of resistance to metacycline was 18.8%. The tetracycline resistance gene tetK was present in all isolates (n = 32), whereas the tetM gene was identified in 12.5% and 9.4% of the isolates, in the chromosome and plasmid, respectively. Strains carrying tetW were also common in the chromosome and plasmid, with abundances of 53.1% and 46.9%, respectively. However, tetO and otrA were absent in all isolates. The resistance phenotype analysis indicated that 6.3% of strains were susceptible to all tetracyclines, while 3.1% showed resistance to all tetracyclines.
authors.affiliation: Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR China.
authors.forename: Dexian
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Jianping Livestock Product Safety Supervision, Chaoyang 122400, PR China.
authors.forename: Jingcui
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Liaoyang Animal Health Supervision Institute, Liaoyang 111000, PR China.
authors.forename: Qiuxia
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR China.
authors.forename: Yaochuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR China.
authors.forename: Chunlian
authors.initials: C
authors.lastname: Tian
authors.affiliation: Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR China.
authors.forename: Yujun
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR China.
authors.forename: Lihui
authors.initials: L
authors.lastname: Yu
authors.affiliation: Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR China. Electronic address: liumingchun@sina.com.
authors.forename: Mingchun
authors.initials: M
authors.lastname: Liu
mesh_terms: D000191:Actinomycetaceae / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000382:microbiology*; D002681:China / Q000453:epidemiology; D002876:Chromosomes, Bacterial; D004716:Endometritis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D005260:Female; D005798:Genes, Bacterial; D010957:Plasmids; D015995:Prevalence; D013753:Tetracycline Resistance; D013754:Tetracyclines / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D013754:Tetracyclines
keywords: Dairy cows with endometritis; Tetracycline resistance; Tetracycline-resistant genes; Trueperella pyogenes