title: Glutathione is a highly efficient thermostabilizer of poliovirus Sabin strains.
abstract: Glutathione (GSH) is the most abundant thiol peptide in animal cells and has a critical role in antioxidation. GSH was reported to be essential for stabilization of some enteroviruses, including poliovirus (PV), during viral morphogenesis. Here, we explored the potential use of GSH as a thermostabilizer of oral poliomyelitis vaccine (OPV) formulations. GSH significantly protected the three types of PV from heat-inactivation in a concentration-dependent manner. At a GSH concentration of 20mM, nearly complete protection was observed against heating temperatures up to 53°C for 2min.GSH also markedly protected PV1 from heat-inactivation and this up to 6 h at temperatures of 44°C and 46°C and 3 h at 48°C. The fact that GSH is naturally present at high concentration in the human body makes it an efficient candidate stabilizer for OPV formulations.
authors.affiliation: KU Leuven - University of Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy, B-3000 Leuven, Belgium.
authors.forename: Rana
authors.initials: R
authors.lastname: Abdelnabi
authors.affiliation: KU Leuven - University of Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy, B-3000 Leuven, Belgium.
authors.forename: Leen
authors.initials: L
authors.lastname: Delang
authors.affiliation: KU Leuven - University of Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy, B-3000 Leuven, Belgium. Electronic address: Johan.Neyts@kuleuven.be.
authors.forename: Johan
authors.initials: J
authors.lastname: Neyts
mesh_terms: D004355:Drug Stability; D005079:Excipients / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*; D050296:Microbial Viability / Q000528:radiation effects; D017955:Poliovirus / Q000502:physiology / Q000528:radiation effects; D011055:Poliovirus Vaccine, Oral / Q000737:chemistry* / Q000276:immunology*; D013696:Temperature; D038301:Virus Inactivation
chemical_list: D005079:Excipients; D011055:Poliovirus Vaccine, Oral; D005978:Glutathione
keywords: Cold chain; Glutathione; Poliomyelitis; Sabin; Vaccine