title: Influence of iodine in excess on seminiferous tubular structure and epididymal sperm character in male rats.
abstract: Excess iodine induced public health problems are now emerging in many iodine sufficient regions for indiscriminate intake of iodine through various iodized products. It has been reported that excess iodine can disrupt overall male reproductive physiology by generating oxidative stress in the testis. However, information on the possible effect of iodine in excess on spermatozoa found less. In the present investigation flow cytometric techniques and scanning electron microscopy (SEM) have been used to study the spermatozoal functional as well as structural status under the influence of excess iodine; generation of ROS in the spermatozoa as evident by DCFDA, altered acrosomal integrity as observed by fluorescence lectin staining method and depolarized mitochondrial membrane potential (ΔΨm) noticed by JC-1 staining. Ultrastructure of seminiferous tubule after excess iodine exposure indicated severe deterioration of seminiferous tubular surface architecture. Significant increase in spermatozoal DNA fragmentation and apoptotic sperms were found by acridine orange and Annexin V, respectively, however the plasma membrane integrity/viability was decreased as evident by propidium iodide staining in various incremental doses and durations under iodine excess. The study reveals that excess iodine could cause apoptosis of spermatozoal cells by inducing ROS that ultimately affects male fertility potential.
authors.affiliation: Endocrinology and Reproductive Physiology Laboratory, Department of Physiology, University College of Science and Technology, University of Calcutta, 92, Acharya Prafulla Chandra Road, Kolkata, West Bengal, 700 009, India.
authors.forename: Amar K
authors.initials: AK
authors.lastname: Chandra
authors.affiliation: Endocrinology and Reproductive Physiology Laboratory, Department of Physiology, University College of Science and Technology, University of Calcutta, 92, Acharya Prafulla Chandra Road, Kolkata, West Bengal, 700 009, India.
authors.forename: Arijit
authors.initials: A
authors.lastname: Chakraborty
mesh_terms: D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D053938:DNA Fragmentation / Q000187:drug effects; D004822:Epididymis / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D005434:Flow Cytometry; D005452:Fluoresceins; D007455:Iodine / Q000633:toxicity*; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D017208:Rats, Wistar; D012671:Seminiferous Tubules / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D013737:Testis / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D017304:Annexin A5; D005452:Fluoresceins; C029569:diacetyldichlorofluorescein; D007455:Iodine
keywords: DNA fragmentation; ROS; SEM; apoptosis; cell cycle; spermatozoa