title: A new natural source for obtainment of inulin and fructo-oligosaccharides from industrial waste of Stevia rebaudiana Bertoni.
abstract: Fructan-type inulin and fructo-oligosaccharides (FOS) are reserve polysaccharides that offer an interesting combination of nutritional and technological properties for food industry. Stevia rebaudiana is used commercially in the sweetener industry due to the high content of steviol glycosides in its leaves. With the proposal of using industrial waste, the objective of the present study was to isolate, characterize and evaluate the prebiotic activity of inulin and FOS from S. rebaudiana stems. The chemical characterization of the samples by GC-MS, NMR and off-line ESI-MS showed that it was possible to obtain inulin molecules from the S. rebaudiana stems with a degree of polymerization (DP) of 12, and FOS with a DP<6. The in vitro prebiotic assay of these molecules indicates a strain specificity in fermentation capacity of fructans as substrate. FOS molecules with a low DP are preferably fermented by beneficial microbiota than inulin molecules with higher DP.
authors.affiliation: Graduate Program in Pharmaceutical Sciences, Department of Pharmacy, State University of Maringá, Ave. Colombo 5790, 87.020-900 Maringá, Brazil.
authors.forename: Sheila Mara Sanches
authors.initials: SM
authors.lastname: Lopes
authors.affiliation: Department of Microbiology and Technology, Dairy Research Institute, Ke Dvoru 12a, 160 00 Prague, Czech Republic.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Krausová
authors.affiliation: Department of Agronomy, Iguatemi Research Farm, State University of Maringá, Av. Colombo 5790, 87.020-900 Maringá, Brazil.
authors.forename: José Walter Pedroza
authors.initials: JW
authors.lastname: Carneiro
authors.affiliation: Program of Master in Health Promotion and Program of Master in Clean Technologies, University Center of Maringá, Av. Guedner, 1610, 87.050-900, Maringá, Brazil.
authors.forename: José Eduardo
authors.initials: JE
authors.lastname: Gonçalves
authors.affiliation: Graduate Program in Pharmaceutical Sciences, Department of Pharmacy, State University of Maringá, Ave. Colombo 5790, 87.020-900 Maringá, Brazil.
authors.forename: Regina Aparecida Correia
authors.initials: RA
authors.lastname: Gonçalves
authors.affiliation: Graduate Program in Pharmaceutical Sciences, Department of Pharmacy, State University of Maringá, Ave. Colombo 5790, 87.020-900 Maringá, Brazil. Electronic address: ajboliveira@uem.br.
authors.forename: Arildo José Braz
authors.initials: AJ
authors.lastname: de Oliveira
mesh_terms: D002236:Carbohydrate Conformation; D005285:Fermentation; D007220:Industrial Waste / Q000032:analysis*; D007444:Inulin / Q000737:chemistry* / Q000378:metabolism; D064307:Microbiota; D036625:Stevia / Q000737:chemistry*
chemical_list: D007220:Industrial Waste; D007444:Inulin
keywords: Chlorotrimethylsilane (PubChem CID: 6397); Ethanol (PubChem CID: 702); Fructo-oligosaccharides; Fructose (PubChem CID: 5984); Glucose (PubChem CID: 5793); Hexamethyldisilazane (PubChem CID: 13838); Industrial waste; Inulin; Inulin from chicory (PubChem CID: 16219508); Myo-inositol (PubChem CID: 892); Prebiotic activity; Pyridine (PubChem CID: 1049).; Stevia rebaudiana; Trifluoroacetic acid (PubChem CID: 6422)