title: Effect of GnRHa ovulation trigger dose on follicular fluid characteristics and granulosa cell gene expression profiles.
abstract: OBJECTIVE
  A recent dose-finding study showed no significant differences in number of mature oocytes, embryos and top-quality embryos when triptorelin doses of 0.2, 0.3 or 0.4 mg were used to trigger final oocyte maturation in oocyte donors co-treated with a gonadotropin-releasing hormone (GnRH) antagonist. This analysis investigated whether triptorelin dosing for triggering final oocyte maturation in oocyte donors induced differences in follicular fluid (FF) hormone levels and granulosa cell gene expression.
  
  
  METHODS
  This single-centre, randomised, parallel, investigator-blinded trial was conducted in oocyte donors undergoing a single stimulation cycle at IVFMD, My Duc Hospital, Ho Chi Minh City, Vietnam, from August 2014 to March 2015. A total of 165 women aged 18-35 years with body mass index <28 kg/m2, anti-Müllerian hormone >1.25 ng/mL, and antral follicle count ≥6 were randomised to three different triptorelin doses for trigger. The main outcome was concentration of steroid hormones in FF collected from the first punctured follicle on each side. Moreover, luteinising hormone receptor (LHR), 3β-hydroxy-steroid-dehydrogenase (3ßHSD) and inhibin-Ba (INHB-A) gene expression in cumulus and mural granulosa cells were investigated in a subset of women from each group.
  
  
  RESULTS
  Progesterone and oestradiol levels in FF did not differ significantly by trigger doses; findings were similar for 3βHSD, LHR and INHB-A gene expression in both cumulus and mural granulosa cells.
  
  
  CONCLUSIONS
  In women co-treated with a GnRH antagonist, no significant differences in FF steroid levels and granulosa cell gene expression were seen when different triptorelin doses were used to trigger final oocyte maturation.
authors.affiliation: Department of Obstetrics and Gynaecology, University of Medicine and Pharmacy HCMC, 217 Hong Bang Street, District 5, Ho Chi Minh City, Vietnam. drlan@yahoo.com.vn.|IVFMD, My Duc Hospital, 4 Nui Thanh Street, Tan Binh District, Ho Chi Minh City, Vietnam. drlan@yahoo.com.vn.
authors.forename: Thi Ngoc Lan
authors.initials: TNL
authors.lastname: Vuong
authors.affiliation: IVFMD, My Duc Hospital, 4 Nui Thanh Street, Tan Binh District, Ho Chi Minh City, Vietnam.|Research Center for Genetics and Reproductive Health (CGRH), School of Medicine, Vietnam National University HCMC, Room 608, VNU-HCM Administrative Building, Quarter 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam.
authors.forename: M T
authors.initials: MT
authors.lastname: Ho
authors.affiliation: IVFMD, My Duc Hospital, 4 Nui Thanh Street, Tan Binh District, Ho Chi Minh City, Vietnam.
authors.forename: T Q
authors.initials: TQ
authors.lastname: Ha
authors.affiliation: Laboratory of Reproductive Biology, The Copenhagen University Hospital and Faculty of Health Science, Copenhagen University, Blegda msvej 9, 2100, Copenhagen, Denmark.
authors.forename: M Brehm
authors.initials: MB
authors.lastname: Jensen
authors.affiliation: Laboratory of Reproductive Biology, The Copenhagen University Hospital and Faculty of Health Science, Copenhagen University, Blegda msvej 9, 2100, Copenhagen, Denmark.
authors.forename: C Yding
authors.initials: CY
authors.lastname: Andersen
authors.affiliation: The Fertility Clinic, Skive Regional Hospital, Resenvej 25, 7800, Skive, Denmark.|Faculty of Health, Aarhus University and Faculty of Health, University of Southern Denmark, Brendstrupgårdsvej 100, 8200, Aarhus, Denmark.
authors.forename: P
authors.initials: P
authors.lastname: Humaidan
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics; D000328:Adult; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D007987:Gonadotropin-Releasing Hormone / Q000037:antagonists & inhibitors* / Q000235:genetics; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D028322:Inhibin-beta Subunits / Q000235:genetics; D010060:Ovulation / Q000187:drug effects*; D010062:Ovulation Induction; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism; D011974:Receptors, LH / Q000235:genetics; D017329:Triptorelin Pamoate / Q000008:administration & dosage*
chemical_list: D011974:Receptors, LH; C103008:inhibin beta A subunit; D017329:Triptorelin Pamoate; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D004958:Estradiol; D028322:Inhibin-beta Subunits; D015096:3-Hydroxysteroid Dehydrogenases
keywords: Follicular fluid; Gonadotropin-releasing hormone agonist trigger; In vitro fertilisation; LHR gene expression; Triptorelin