title: Photo-Induced Click Chemistry for DNA Surface Structuring by Direct Laser Writing.
abstract: Oligonucleotides containing photo-caged dienes were prepared and shown to react quantitatively in a light-induced Diels-Alder cycloaddition with functional maleimides in aqueous solution within minutes. Due to its high yield and fast rate, the reaction was exploited for DNA surface patterning with sub-micrometer resolution employing direct laser writing (DLW). Functional DNA arrays were written by direct laser writing (DLW) in variable patterns, which were further encoded with fluorophores and proteins through DNA directed immobilization. This mild and efficient light-driven platform technology holds promise for the fabrication of complex bioarrays with sub-micron resolution.
authors.affiliation: Department of Chemical Engineering and Biotechnology, University of Cambridge, New Museums Site, Pembroke Street, Cambridge, CB2 3RA, UK.
authors.forename: Antonina
authors.initials: A
authors.lastname: Kerbs
authors.affiliation: Institute of Nanotechnology, Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
authors.forename: Patrick
authors.initials: P
authors.lastname: Mueller
authors.affiliation: Preparative Macromolecular Chemistry, Institute for Technical and Polymer Chemistry, Karlsruhe Institute of Technology (KIT), Engesserstrasse 18, 76131, Karlsruhe, Germany.|Institute for Biological Interfaces 3, Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Kaupp
authors.affiliation: Institute for Biological Interfaces 1, Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
authors.forename: Ishtiaq
authors.initials: I
authors.lastname: Ahmed
authors.affiliation: Preparative Macromolecular Chemistry, Institute for Technical and Polymer Chemistry, Karlsruhe Institute of Technology (KIT), Engesserstrasse 18, 76131, Karlsruhe, Germany.|Institute for Biological Interfaces 3, Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Quick
authors.affiliation: Preparative Macromolecular Chemistry, Institute for Technical and Polymer Chemistry, Karlsruhe Institute of Technology (KIT), Engesserstrasse 18, 76131, Karlsruhe, Germany.|Institute for Biological Interfaces 3, Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
authors.forename: Doris
authors.initials: D
authors.lastname: Abt
authors.affiliation: Institute of Nanotechnology, Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Wegener
authors.affiliation: Institute for Biological Interfaces 1, Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
authors.forename: Christof M
authors.initials: CM
authors.lastname: Niemeyer
authors.affiliation: Preparative Macromolecular Chemistry, Institute for Technical and Polymer Chemistry, Karlsruhe Institute of Technology (KIT), Engesserstrasse 18, 76131, Karlsruhe, Germany.|Institute for Biological Interfaces 3, Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
authors.forename: Christopher
authors.initials: C
authors.lastname: Barner-Kowollik
authors.affiliation: Department of Chemical Engineering and Biotechnology, University of Cambridge, New Museums Site, Pembroke Street, Cambridge, CB2 3RA, UK.
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Fruk
mesh_terms: D057930:Click Chemistry; D061565:Cycloaddition Reaction; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D007834:Lasers; D008027:Light; D008301:Maleimides / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D008301:Maleimides; D004247:DNA
keywords: DNA microarrays; DNA-directed immobilization; Diels-Alder cycloaddition; direct laser writing (DLW); light-active oligonucleotides