title: Simultaneous Detection of Dual Nucleic Acids Using a SERS-Based Lateral Flow Assay Biosensor.
abstract: A new class of surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) biosensor has been developed for the simultaneous detection of dual DNA markers. The LFA strip in this sensor was composed of two test lines and one control line. SERS nano tags labeled with detection DNA probes were used for quantitative evaluation of dual DNA markers with high sensitivity. Target DNA, associated with Kaposi's sarcoma-associated herpesvirus (KSHV) and bacillary angiomatosis (BA), were tested to validate the detection capability of this SERS-based LFA strip. Characteristic peak intensities of SERS nano tags on two test lines were used for quantitative evaluations of KSHV and BA. The limits of detection for KSHV and BA, determined from our SERS-based LFA sensing platform, were estimated to be 0.043 and 0.074 pM, respectively. These values indicate approximately 10 000 times higher sensitivity than previously reported values using the aggregation-based colorimetric method. We believe that this is the first report of simultaneous detection of two different DNA mixtures using a SERS-based LFA platform. This novel detection technique is also a promising multiplex DNA sensing platform for early disease diagnosis.
authors.affiliation: Department of Bionano Engineering, Hanyang University , Ansan 426-791, South Korea.
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Bionano Engineering, Hanyang University , Ansan 426-791, South Korea.
authors.forename: Namhyun
authors.initials: N
authors.lastname: Choi
authors.affiliation: Department of Bionano Engineering, Hanyang University , Ansan 426-791, South Korea.
authors.forename: Ziyi
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: Department of Bionano Engineering, Hanyang University , Ansan 426-791, South Korea.
authors.forename: Juhui
authors.initials: J
authors.lastname: Ko
authors.affiliation: Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences , Yantai 264003, China.
authors.forename: Lingxin
authors.identifier: 0000-0002-3764-3515
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Bionano Engineering, Hanyang University , Ansan 426-791, South Korea.
authors.forename: Jaebum
authors.identifier: 0000-0003-3864-6459
authors.initials: J
authors.lastname: Choo
mesh_terms: D016917:Angiomatosis, Bacillary / Q000382:microbiology*; D018416:Bartonella henselae / Q000235:genetics / Q000302:isolation & purification; D015374:Biosensing Techniques / Q000295:instrumentation*; D004247:DNA / Q000032:analysis* / Q000235:genetics; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D004867:Equipment Design; D006566:Herpesviridae Infections / Q000821:virology*; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D011934:Reagent Strips / Q000032:analysis; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D004279:DNA, Viral; D011934:Reagent Strips; D004247:DNA