title: The cryoprotective effect of iodixanol in buffalo semen cryopreservation.
abstract: This is the first report to examine the effect of iodixanol (OptiPrepTM) on cryosurvival of buffalo spermatozoa. A total of thirty ejaculates (five ejaculates from each bull) from six buffalo bulls were used for this experiment. Each ejaculate was divided into four aliquots and diluted in freezing extender supplemented with different concentrations of OptiPrepTM(0, 1.25, 2.5 and 5%) and then cryopreserved. The semen quality variables were evaluated before and after freezing of the semen. There were no effects of OptiPrepTM(P>0.05) on sperm kinetics, motility, abnormality and membrane integrity of fresh extended spermatozoa. However, after freeze-thaw, sperm motility, plasma membrane integrity and distance travelled in cervical mucus of 2.5% OptiPrepTMtreated samples showed significantly higher (P<0.05) compared to other treated and control samples. No significant differences (P>0.05) were seen in sperm abnormality and acrosomal integrity of treated and control frozen-thawed samples. The total antioxidant capacity of 2.5 and 5% OptiPrepTMtreated frozen-thawed sperm were found to be higher (P<0.05) as compared to other groups; whereas the MDA level in OptiPrepTMtreated sperm was significantly lower than the control (P<0.05). In incubation test, 2.5% OptiPrepTMproved to be better in preservation of sperm motility as compared to other treated and control samples. In conclusion, the present study has shown that iodixanol has the ability protect spermatozoa against oxidative stress and resulting overall improvement in the post-thaw semen quality.
authors.affiliation: ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana, India; Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India.
authors.forename: Dheer Singh
authors.initials: DS
authors.lastname: Swami
authors.affiliation: ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana, India. Electronic address: drpradeepkrvet@gmail.com.
authors.forename: Pradeep
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India.
authors.forename: R K
authors.initials: RK
authors.lastname: Malik
authors.affiliation: ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana, India.
authors.forename: Monika
authors.initials: M
authors.lastname: Saini
authors.affiliation: ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana, India.
authors.forename: Dharmendra
authors.initials: D
authors.lastname: Kumar
authors.affiliation: ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana, India.
authors.forename: M H
authors.initials: MH
authors.lastname: Jan
mesh_terms: D000818:Animals; D002020:Buffaloes; D015925:Cryopreservation / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D008297:Male; D012661:Semen / Q000187:drug effects*; D012662:Semen Preservation / Q000662:veterinary*; D014283:Triiodobenzoic Acids / Q000494:pharmacology*
chemical_list: D003451:Cryoprotective Agents; D014283:Triiodobenzoic Acids; C044834:iodixanol
keywords: Buffalo; CASA; Iodixanol; OptiPrep(TM); Oxidative stress; Semen