title: Automated microfluidic sorting of mammalian cells labeled with magnetic microparticles for those that efficiently express and secrete a protein of interest.
abstract: We developed a method for the fast sorting and selection of mammalian cells expressing and secreting a protein at high levels. This procedure relies on cell capture using an automated microfluidic device handling antibody-coupled magnetic microparticles and on a timed release of the cells from the microparticles after capture. Using clinically compatible materials and procedures, we show that this approach is able to discriminate between cells that truly secrete high amounts of a protein from those that just display it at high levels on their surface without properly releasing it. When coupled to a cell colony imaging and picking device, this approach allowed the identification of CHO cell clones secreting a therapeutic protein at high levels that were not achievable without the cell sorting procedure. Biotechnol. Bioeng. 2017;114: 1791-1802. © 2017 Wiley Periodicals, Inc.
authors.affiliation: Department of Fundamental Microbiology, Institute of Biotechnology, University of Lausanne, Center for Biotechnology UNIL-EPFL, Lausanne, Switzerland.
authors.forename: Xuan
authors.initials: X
authors.lastname: Droz
authors.affiliation: Department of Fundamental Microbiology, Institute of Biotechnology, University of Lausanne, Center for Biotechnology UNIL-EPFL, Lausanne, Switzerland.
authors.forename: Niamh
authors.initials: N
authors.lastname: Harraghy
authors.affiliation: Department of Fundamental Microbiology, Institute of Biotechnology, University of Lausanne, Center for Biotechnology UNIL-EPFL, Lausanne, Switzerland.
authors.forename: Etienne
authors.initials: E
authors.lastname: Lançon
authors.affiliation: Selexis SA, Geneva, Switzerland.
authors.forename: Valérie
authors.initials: V
authors.lastname: Le Fourn
authors.affiliation: Selexis SA, Geneva, Switzerland.
authors.forename: David
authors.initials: D
authors.lastname: Calabrese
authors.affiliation: Spinomix SA, Ecublens, Switzerland.
authors.forename: Thierry
authors.initials: T
authors.lastname: Colombet
authors.affiliation: Spinomix SA, Ecublens, Switzerland.
authors.forename: Pascal
authors.initials: P
authors.lastname: Liechti
authors.affiliation: Spinomix SA, Ecublens, Switzerland.
authors.forename: Amar
authors.initials: A
authors.lastname: Rida
authors.affiliation: Selexis SA, Geneva, Switzerland.
authors.forename: Pierre-Alain
authors.initials: PA
authors.lastname: Girod
authors.affiliation: Department of Fundamental Microbiology, Institute of Biotechnology, University of Lausanne, Center for Biotechnology UNIL-EPFL, Lausanne, Switzerland.
authors.forename: Nicolas
authors.identifier: 0000-0001-7741-3186
authors.initials: N
authors.lastname: Mermod
mesh_terms: D000818:Animals; D016466:CHO Cells / Q000166:cytology* / Q000378:metabolism* / Q000528:radiation effects; D002469:Cell Separation / Q000379:methods*; D003412:Cricetulus; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000528:radiation effects; D011994:Recombinant Proteins / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods
chemical_list: D058185:Magnetite Nanoparticles; D011994:Recombinant Proteins
keywords: CHO cells; magnetic microparticles; microfluidic cell sorting; protein secretion; recombinant protein expression