title: In vitro oral bioaccessibility and total content of Cu, Fe, Mn and Zn from transgenic (through cp4 EPSPS gene) and nontransgenic precursor/successor soybean seeds.
abstract: In this paper, Cu, Fe, Mn and Zn contents in transgenic (T - MSOY7122RR) and non-transgenic (NT - MSOY8200) soybean seeds, sown at summer and winter cultivation periods are investigated using four microwave decomposition methods. Student's t tests demonstrate significant differences (p=0.05; n=4), for Cu, Mn and Zn (namely, 8, 9 and 26% higher concentrations in T compared to NT seeds, respectively). Through principal component analysis, precursor and successor soybean seeds are identified. Cu is demonstrated to play an important role in the differentiation of the cultivars, whereas Fe and Zn are of particular relevance in the classification of seeds cultivated in winter against those in summer. Using in vitro extraction based on the Unified Bioaccessibility Method, the bioaccessibility of the above nutrients is proven to differ in both the gastric and gastrointestinal phases on the basis of the transgenesis and the cultivation periods.
authors.affiliation: Spectrometry, Sample Preparation and Mechanization Group, Institute of Chemistry, University of Campinas - Unicamp, P.O. Box 6154, Campinas, SP 13083-970, Brazil; National Institute of Science and Technology for Bioanalytics, Institute of Chemistry, University of Campinas - Unicamp, P.O. Box 6154, Campinas, SP 13083-970, Brazil.
authors.forename: Mónica A
authors.initials: MA
authors.lastname: Herrera-Agudelo
authors.affiliation: FI-TRACE Group, Department of Chemistry, Faculty of Sciences, University of the Balearic Islands, Carretera de Valldemossa km.7.5, E-07122 Palma de Mallorca, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Miró
authors.affiliation: Spectrometry, Sample Preparation and Mechanization Group, Institute of Chemistry, University of Campinas - Unicamp, P.O. Box 6154, Campinas, SP 13083-970, Brazil; National Institute of Science and Technology for Bioanalytics, Institute of Chemistry, University of Campinas - Unicamp, P.O. Box 6154, Campinas, SP 13083-970, Brazil. Electronic address: zezzi@iqm.unicamp.br.
authors.forename: Marco A Z
authors.initials: MA
authors.lastname: Arruda
mesh_terms: D000818:Animals; D001682:Biological Availability; D003300:Copper / Q000378:metabolism; D004063:Digestion; D007501:Iron / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D018977:Micronutrients / Q000378:metabolism*; D030821:Plants, Genetically Modified; D012639:Seeds / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry*; D015032:Zinc / Q000378:metabolism
chemical_list: D018977:Micronutrients; D008345:Manganese; D003300:Copper; D007501:Iron; D015032:Zinc
keywords: Micro-minerals; Oral bioaccessibility; Soybean; Transgenesis