title: ALD5, PAD1, ATF1 and ATF2 facilitate the catabolism of coniferyl aldehyde, ferulic acid and p-coumaric acid in Saccharomyces cerevisiae.
abstract: The ability of Saccharomyces cerevisiae to catabolize phenolic compounds remains to be fully elucidated. Conversion of coniferyl aldehyde, ferulic acid and p-coumaric acid by S. cerevisiae under aerobic conditions was previously reported. A conversion pathway was also proposed. In the present study, possible enzymes involved in the reported conversion were investigated. Aldehyde dehydrogenase Ald5, phenylacrylic acid decarboxylase Pad1, and alcohol acetyltransferases Atf1 and Atf2, were hypothesised to be involved. Corresponding genes for the four enzymes were overexpressed in a S. cerevisiae strain named APT_1. The ability of APT_1 to tolerate and convert the three phenolic compounds was tested. APT_1 was also compared to strains B_CALD heterologously expressing coniferyl aldehyde dehydrogenase from Pseudomonas, and an ald5Δ strain, all previously reported. APT_1 exhibited the fastest conversion of coniferyl aldehyde, ferulic acid and p-coumaric acid. Using the intermediates and conversion products of each compound, the catabolic route of coniferyl aldehyde, ferulic acid and p-coumaric acid in S. cerevisiae was studied in greater detail.
authors.affiliation: Department of Biology and Biological Engineering, Division of Industrial Biotechnology, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden.
authors.forename: Peter Temitope
authors.initials: PT
authors.lastname: Adeboye
authors.affiliation: Department of Biology and Biological Engineering, Division of Industrial Biotechnology, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Bettiga
authors.affiliation: Department of Biology and Biological Engineering, Division of Industrial Biotechnology, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden.
authors.forename: Lisbeth
authors.initials: L
authors.lastname: Olsson
mesh_terms: D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D000171:Acrolein / Q000031:analogs & derivatives* / Q000378:metabolism; D000445:Aldehyde Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D002262:Carboxy-Lyases / Q000235:genetics / Q000378:metabolism*; D003373:Coumaric Acids / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D060847:Metabolic Engineering; D000439:Pentanols / Q000378:metabolism; D011422:Propionates / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D003373:Coumaric Acids; D000439:Pentanols; D011422:Propionates; D011506:Proteins; D029701:Saccharomyces cerevisiae Proteins; C075384:coniferaldehyde; D000171:Acrolein; C004999:ferulic acid; C029683:isopentyl alcohol; D000445:Aldehyde Oxidoreductases; D000123:Acetyltransferases; C116217:ATF2 protein, S cerevisiae; C079238:alcohol O-acetyltransferase; D002262:Carboxy-Lyases; C087194:phenylacrylic acid decarboxylase; C495469:p-coumaric acid