title: Pneumococcal Predictive Proteins Selected by Microbial Genomic Approach Are Serotype Cross-Reactive and Bind to Host Extracellular Matrix Proteins.
abstract: Streptococcus pneumoniae is a colonizer of the human nasopharynx, which accounts for most of the community-acquired pneumonia cases and can cause non-invasive and invasive diseases. Current available vaccines are serotype-specific and the use of recombinant proteins associated with virulence is an alternative to compose vaccines and to overcome these problems. In a previous work, we describe the identification of proteins in S. pneumoniae by reverse vaccinology and the genetic diversity of these proteins in clinical isolates. It was possible to purify a half of 20 selected proteins in soluble form. The expression of these proteins on the pneumococcal cells surface was confirmed by flow cytometry. We demonstrated that some of these proteins were able to bind to extracellular matrix proteins and were recognized by sera from patients with pneumococcal meningitis infection caused by several pneumococcal serotypes. In this context, our results suggest that these proteins may play a role in pneumococcal pathogenesis and might be considered as potential vaccine candidates.
authors.affiliation: Laboratory of Recombinant Technology, Bio-Manguinhos, Brazilian Health Ministry, FIOCRUZ, Rio de Janeiro, Brazil. acorrea@bio.fiocruz.br.
authors.forename: Ana Paula Corrêa
authors.initials: APC
authors.lastname: Argondizzo
authors.affiliation: Research Laboratory of Hospital Infection, Collection Hospital Origin bacteria cultures, Instituto Oswaldo Cruz, Brazilian Health Ministry, FIOCRUZ, Rio de Janeiro, Brazil.
authors.forename: Cláudio Marcos
authors.initials: CM
authors.lastname: Rocha-de-Souza
authors.affiliation: Laboratory of Diagnostic Technology, Bio-Manguinhos, Brazilian Health Ministry, FIOCRUZ, Rio de Janeiro, Brazil.
authors.forename: Marta
authors.initials: M
authors.lastname: de Almeida Santiago
authors.affiliation: Fiocruz, Bio-Manguinhos, Brazilian Health Ministry, Rio de Janeiro, Brazil.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Galler
authors.affiliation: School of Pharmacy, Federal University of Bahia, Salvador, BA, Brazil.
authors.forename: Joice Neves
authors.initials: JN
authors.lastname: Reis
authors.affiliation: Laboratory of Recombinant Technology, Bio-Manguinhos, Brazilian Health Ministry, FIOCRUZ, Rio de Janeiro, Brazil.
authors.forename: Marco Alberto
authors.initials: MA
authors.lastname: Medeiros
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D003001:Cloning, Molecular; D003429:Cross Reactions; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D023281:Genomics; D051379:Mice; D022242:Pneumococcal Vaccines / Q000276:immunology; D011485:Protein Binding; D065288:Serogroup; D013296:Streptococcus pneumoniae / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D016326:Extracellular Matrix Proteins; D022242:Pneumococcal Vaccines
keywords: Cross-reactivity; Extracellular matrix proteins; Streptococcus pneumoniae; Surface-exposed pneumococcal proteins; Vaccine