title: Structural and functional changes to lymph nodes in ageing mice.
abstract: Lymph nodes (LN) are secondary lymphoid organs spread throughout the lymphatic system. They function to filter pathogenic material from the lymphatic fluid to maintain the health of the organism. Subcapsular sinus macrophages (SCSM) are among the first-responders within the LN due to their strategic location within the subcapsular sinus region. These macrophages aid the delivery of immune complexes to B cells and follicular dendritic cells (FDC) within the LN. Here we show an increase in SCSM and other macrophage populations within aged LN. However, immune complex uptake by macrophages within LN was not altered with age, nor was immune complex uptake by B cells. LN stromal cell populations, important in immune responses and the localization and survival of leucocytes, were altered in their representation and distribution in aged LN. In particular, FDC regions were decreased in size and had decreased chemokine CXCL13 expression. Furthermore, the retention of immune complexes by FDC was decreased in aged LN at 24 hr post-injection. As FDC are important in the maintenance of germinal centre responses, the decreased retention of immune complex in aged LN may contribute to the reduced germinal centre responses observed in aged mice.
authors.affiliation: The Roslin Institute and Royal (Dick) School of Veterinary Sciences, University of Edinburgh, Midlothian, UK.
authors.forename: Vivian M
authors.identifier: 0000-0002-2013-2559
authors.initials: VM
authors.lastname: Turner
authors.affiliation: The Roslin Institute and Royal (Dick) School of Veterinary Sciences, University of Edinburgh, Midlothian, UK.
authors.forename: Neil A
authors.identifier: 0000-0001-7395-1796
authors.initials: NA
authors.lastname: Mabbott
mesh_terms: D000375:Aging / Q000276:immunology*; D000818:Animals; D005260:Female; D008198:Lymph Nodes / Q000033:anatomy & histology* / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL
keywords: ageing; follicular dendritic cells; lymph node; macrophages; stromal cells