title: Wholegrain oat diet changes the expression of genes associated with intestinal bile acid transport.
abstract: The molecular mechanisms underlying the cholesterol-lowering properties of oats are only partly known. To study possible pathways involved, we investigated gene expressions in the liver and small intestine of mice fed oats.
  
  
  
  Cholesterol and bile acids were analyzed in plasma and feces from LDL-receptor deficient (LDLr-/-) mice fed Western diet with wholegrain oats. A transcriptome analysis of mRNA from liver and jejunum was performed together with quantitative RT-PCR. Oat-fed mice had lower levels of plasma lipids and increased levels of bile acids and cholesterol in feces compared with controls. Two hundred thirty nine genes in jejunum and 25 genes in liver were differentially expressed (FDR corrected p < 0.05). The most affected biological process in jejunum was lipid biosynthesis and regulation. The apical sodium-dependent bile acid transporter (ASBT, Slc10a) and the intracellular bile acid binding protein (Fabp6) were both upregulated, whereas small heterodimer partner-1 (Shp-1) and apolipoprotein CII (Apoc2) were downregulated.
  
  
  
  Whole oats attenuated responses typically induced by high-fat diet. Increased expression of genes for intestinal bile acid uptake following oat consumption suggests retention in the gut lumen rather than decreased uptake capacity as cause for the increased bile acid excretion and the concomitant reduction of plasma cholesterol.
authors.affiliation: Department of Experimental Medical Science, Lund University, Sweden.
authors.forename: Kristina E
authors.initials: KE
authors.lastname: Andersson
authors.affiliation: Department of Plant Breeding, Swedish University of Agricultural Sciences, Alnarp, Sweden.
authors.forename: Aakash
authors.initials: A
authors.lastname: Chawade
authors.affiliation: Department of Chemistry, Lund University, Sweden.
authors.forename: Narda
authors.initials: N
authors.lastname: Thuresson
authors.affiliation: Food for Health Science Centre, Lund University, Sweden.
authors.forename: Ana
authors.initials: A
authors.lastname: Rascon
authors.affiliation: Food for Health Science Centre, Lund University, Sweden.
authors.forename: Rickard
authors.initials: R
authors.lastname: Öste
authors.affiliation: Department of Chemistry, Lund University, Sweden.
authors.forename: Olov
authors.initials: O
authors.lastname: Sterner
authors.affiliation: Department of Pure and Applied Biochemistry, Lund University, Sweden.
authors.forename: Olof
authors.initials: O
authors.lastname: Olsson
authors.affiliation: Department of Experimental Medical Science, Lund University, Sweden.
authors.forename: Per
authors.initials: P
authors.lastname: Hellstrand
mesh_terms: D000818:Animals; D018554:Avena; D001647:Bile Acids and Salts / Q000235:genetics* / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D066273:Diet, Western; D050556:Fatty Acid-Binding Proteins / Q000235:genetics; D005243:Feces; D005260:Female; D020869:Gene Expression Profiling; D007413:Intestinal Mucosa / Q000378:metabolism; D007583:Jejunum / Q000502:physiology*; D008055:Lipids / Q000097:blood; D008099:Liver / Q000502:physiology*; D008817:Mice, Mutant Strains; D029363:Organic Anion Transporters, Sodium-Dependent / Q000235:genetics; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6 / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D011973:Receptors, LDL / Q000235:genetics; D027981:Symporters / Q000235:genetics; D000067348:Whole Grains
chemical_list: D001647:Bile Acids and Salts; C000606532:Fabp6 protein, mouse; D050556:Fatty Acid-Binding Proteins; D008055:Lipids; D029363:Organic Anion Transporters, Sodium-Dependent; D011973:Receptors, LDL; D027981:Symporters; C071908:sodium-bile acid cotransporter; D002784:Cholesterol; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6
keywords: Bile acids; Cholesterol; Diet; Gene expression; Oats