title: A potential panel of four-long noncoding RNA signature in prostate cancer predicts biochemical recurrence-free survival and disease-free survival.
abstract: OBJECTIVE
  Growing evidences showed that lncRNAs abnormally expressed in cancer tissues and played irreplaceable roles in tumorigenesis, progression and metastasis. In present study, we aimed to identify lncRNA expression signature that can predict biochemical recurrence-free (BCR-free) survival of prostate cancer (PCa) patients.
  
  
  METHODS
  A total of 291 patients with pathologic confirmed PCa in The Cancer Genome Atlas dataset were recruited and included. With the specific risk score formula, patients were further classified into high-risk group and low-risk group. Kaplan-Meier survival analyses and Cox regression analyses were performed to determine the association between lncRNA signature and survival outcomes. Gene Set Enrichment Analysis (GSEA) was carried out to identify the potentially associated biological processes and signaling pathway.
  
  
  RESULTS
  Overall, 126 differentially expressed lncRNAs were found with more than twofold changes and p value of FDR <0.01. Among which, four lncRNAs were identified to be significantly associated with BCR-free survival. Then, using a risk score based on the signature of these four lncRNAs, we divided the patients into low-risk and high-risk groups with significantly different BCR-free survival and disease-free survival. Further multivariate Cox regression analyses revealed that the four-lncRNA signature was independent of age, AJCC T stage, lymphonodus status, Gleason score, margin and adjuvant postoperative radiotherapy. GSEA suggested that this signature was involved in cell proliferation.
  
  
  CONCLUSIONS
  In present study, a novel four-lncRNA signature that is useful in survival prediction in PCa patients was developed. If validated, this lncRNA signature might assist in selecting high-risk subpopulation who need more aggressive therapeutic intervention. The clinical implications and the mechanism of these four lncRNAs deserve further investigation in future studies.
authors.affiliation: Department of Urology, Northern Jiangsu People's Hospital, College of Clinical Medicine, Yangzhou University, No. 98 West Nantong Road, Yangzhou, 225001, Jiangsu Province, China.
authors.forename: Tian-Bao
authors.initials: TB
authors.lastname: Huang
authors.affiliation: Institute of Biomedical Science, Shanghai Medical College, Fudan University, Dong'an Rd 131, Xuhui District, Shanghai, China.
authors.forename: Chuan-Peng
authors.initials: CP
authors.lastname: Dong
authors.affiliation: Department of Urology, Northern Jiangsu People's Hospital, College of Clinical Medicine, Yangzhou University, No. 98 West Nantong Road, Yangzhou, 225001, Jiangsu Province, China.
authors.forename: Guang-Chen
authors.initials: GC
authors.lastname: Zhou
authors.affiliation: Department of Urology, Northern Jiangsu People's Hospital, College of Clinical Medicine, Yangzhou University, No. 98 West Nantong Road, Yangzhou, 225001, Jiangsu Province, China.
authors.forename: Sheng-Ming
authors.initials: SM
authors.lastname: Lu
authors.affiliation: Department of Urology, Northern Jiangsu People's Hospital, College of Clinical Medicine, Yangzhou University, No. 98 West Nantong Road, Yangzhou, 225001, Jiangsu Province, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Luan
authors.affiliation: Department of Urology, Northern Jiangsu People's Hospital, College of Clinical Medicine, Yangzhou University, No. 98 West Nantong Road, Yangzhou, 225001, Jiangsu Province, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Gu
authors.affiliation: Institute of Biomedical Science, Shanghai Medical College, Fudan University, Dong'an Rd 131, Xuhui District, Shanghai, China. liulei_sibs@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Urology, Northern Jiangsu People's Hospital, College of Clinical Medicine, Yangzhou University, No. 98 West Nantong Road, Yangzhou, 225001, Jiangsu Province, China. dingxf_sbyy@163.com.
authors.forename: Xue-Fei
authors.initials: XF
authors.lastname: Ding
mesh_terms: D000328:Adult; D000368:Aged; D001707:Biopsy, Needle; D015331:Cohort Studies; D030541:Databases, Genetic; D018572:Disease-Free Survival; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D007564:Japan; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009364:Neoplasm Recurrence, Local / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D011237:Predictive Value of Tests; D011379:Prognosis; D016016:Proportional Hazards Models; D011446:Prospective Studies; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000401:mortality* / Q000473:pathology / Q000628:therapy; D062085:RNA, Long Noncoding / Q000235:genetics*; D012372:ROC Curve; D018570:Risk Assessment; D016019:Survival Analysis; D016896:Treatment Outcome
chemical_list: D062085:RNA, Long Noncoding
keywords: Biochemical recurrence; Gleason score; Long noncoding RNA; Prostate cancer; Signature; Survival