title: Temporal changes of microRNA gga-let-7b and gga-let-7i expression in chickens challenged with subgroup J avian leukosis virus.
abstract: Two important microRNAs, gga-let-7b and gga-let-7i were examined for the relative expression in liver and bone marrow tissues from specific pathogen free chickens that were challenged either with GD1109 or NX0101 strain of subgroup J avian leukosis virus (ALV-J). The GD1109 strain of ALV-J reportedly causes hemangioma (HE) and NX0101 reportedly causes myeloma (ML) in susceptible chickens. Temporal changes of both gga-let-7b and gga-let-7i expression in ALV-J infected chickens were observed in contrast to its counterpart of a non-infected negative control group of chickens (P < 0.05 or P < 0.01) during the first 120 days post infection. Use of the web-based computational DIANA-mirPath software (available at http://microrna.gr/mirpath ), it was predicted that both gga-let-7b and gga-let-7i were involved in multiple pathways including signaling pathways, such as MAPK, TGF-beta, Notch, Wnt, mTOR, Cell cycle, P53 and Jak-STAT. Combining our experimental data with reports on the microRNAs, we suggest that both gga-let-7i and gga-let-7b may also act as tumor suppressors in chicken, especially play a critical role in tumorigenesis induced by ALV-J.
authors.affiliation: China-UK-NYNURRes Joint Laboratory of Insect Biology, Nanyang Normal University, Henan, 473061, People's Republic of China. jijun020@126.com.|Breeding and Reproduction, Ministry of Agriculture, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding and Key Lab of Chicken Genetics, Guangzhou, 510642, People's Republic of China. jijun020@126.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Ji
authors.affiliation: Breeding and Reproduction, Ministry of Agriculture, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding and Key Lab of Chicken Genetics, Guangzhou, 510642, People's Republic of China.
authors.forename: Huiqin
authors.initials: H
authors.lastname: Shang
authors.affiliation: Agriculture Research Service, Avian Disease and Oncology Laboratory, USDA, East Lansing, MI, 48823, USA.
authors.forename: Huanmin
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Breeding and Reproduction, Ministry of Agriculture, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding and Key Lab of Chicken Genetics, Guangzhou, 510642, People's Republic of China.
authors.forename: Hongxin
authors.initials: H
authors.lastname: Li
authors.affiliation: Breeding and Reproduction, Ministry of Agriculture, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding and Key Lab of Chicken Genetics, Guangzhou, 510642, People's Republic of China.|Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangdong Provincial Department of Science and Technology, Guangzhou, 510642, People's Republic of China.
authors.forename: Jingyun
authors.initials: J
authors.lastname: Ma
authors.affiliation: Breeding and Reproduction, Ministry of Agriculture, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding and Key Lab of Chicken Genetics, Guangzhou, 510642, People's Republic of China.|Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangdong Provincial Department of Science and Technology, Guangzhou, 510642, People's Republic of China.
authors.forename: Yingzuo
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Breeding and Reproduction, Ministry of Agriculture, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding and Key Lab of Chicken Genetics, Guangzhou, 510642, People's Republic of China. qmx@scau.edu.cn.|Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangdong Provincial Department of Science and Technology, Guangzhou, 510642, People's Republic of China. qmx@scau.edu.cn.|College of Animal Science, South China Agricultural University, Guangzhou, 510642, People's Republic of China. qmx@scau.edu.cn.
authors.forename: Qingmei
authors.initials: Q
authors.lastname: Xie
mesh_terms: D000818:Animals; D001353:Avian Leukosis / Q000378:metabolism* / Q000821:virology; D001354:Avian Leukosis Virus; D002645:Chickens / Q000378:metabolism / Q000821:virology; D015967:Gene Expression Regulation, Viral; D035683:MicroRNAs / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013997:Time Factors
chemical_list: D035683:MicroRNAs
keywords: ALV-J; Gga-let-7b/7i; Temporal expression; Tumorigenesis; microRNA