title: Programmable DNA triple-helix molecular switch in biosensing applications: from in homogenous solutions to in living cells.
abstract: Herein, we demonstrated a new gold nanoparticles (AuNPs)-integrated programmable triple-helix molecular switch (THMS) to realize the biosensing of multiple targets from in homogenous solution to in living cells. The results demonstrated that this proposed programmable THMS could be successfully used for imaging multiple messenger RNA (mRNA) in living cells and it significantly extends the scope of the THMS sensing platform.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China. zhengjing2013@hnu.edu.cn Yangrh@pku.ed.cn.
authors.forename: Pinting
authors.initials: P
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China. zhengjing2013@hnu.edu.cn Yangrh@pku.ed.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China. zhengjing2013@hnu.edu.cn Yangrh@pku.ed.cn.
authors.forename: Jianru
authors.initials: J
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China. zhengjing2013@hnu.edu.cn Yangrh@pku.ed.cn.
authors.forename: Dandan
authors.initials: D
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China. zhengjing2013@hnu.edu.cn Yangrh@pku.ed.cn.
authors.forename: Weijian
authors.initials: W
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China. zhengjing2013@hnu.edu.cn Yangrh@pku.ed.cn.
authors.forename: Jishan
authors.initials: J
authors.lastname: Li
authors.affiliation: Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation, School of Chemistry and Biological Engineering, Changsha University of Science and Technology, Changsha 410114, P. R. China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Cao
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China. zhengjing2013@hnu.edu.cn Yangrh@pku.ed.cn and Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation, School of Chemistry and Biological Engineering, Changsha University of Science and Technology, Changsha 410114, P. R. China.
authors.forename: Ronghua
authors.identifier: http://orcid.org/0000-0003-1747-5398
authors.initials: R
authors.lastname: Yang
mesh_terms: D020029:Base Pairing; D015374:Biosensing Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics*; D005453:Fluorescence; D006046:Gold / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D018613:Microscopy, Confocal / Q000379:methods; D017350:N-Acetylgalactosaminyltransferases / Q000235:genetics; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D000077022:Survivin; D013937:Thymidine Kinase / Q000235:genetics; D000097763:Polypeptide N-acetylgalactosaminyltransferase
chemical_list: D004247:DNA; D015342:DNA Probes; D006046:Gold; D051034:Inhibitor of Apoptosis Proteins; D017350:N-Acetylgalactosaminyltransferases; D012333:RNA, Messenger; D000077022:Survivin; D013937:Thymidine Kinase; D000097763:Polypeptide N-acetylgalactosaminyltransferase; C107489:BIRC5 protein, human; C092717:thymidine kinase 1