title: Orientin Ameliorates LPS-Induced Inflammatory Responses through the Inhibitory of the NF-κB Pathway and NLRP3 Inflammasome.
abstract: Inflammation is a complex response to diverse pathological conditions, resulting in negative rather than protective effects when uncontrolled. Orientin (Ori), a flavonoid component isolated from natural plants, possesses abundant properties. Thus, we aimed to discover the potential therapeutic effects of orientin on lipopolysaccharide- (LPS-) induced inflammation in RAW 264.7 cells and the underlying mechanisms. In our studies, we evaluated the effects of Ori on proinflammatory mediator production stimulated by LPS, including tumor necrosis factor- (TNF-)α, interleukin- (IL-) 6, IL-18, and IL-1β, along with prostaglandin E2(PGE2) and NO. Our data indicated that orientin dramatically inhibited the levels of these mediators. Consistent with these results, the expression levels of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) were also reduced. Further study demonstrated that such inhibitory effects of Ori were due to suppression of the nuclear factor-kappa B (NF-κB) pathway and nucleotide-binding domain- (NOD-) like receptor protein 3 (NLRP3) inflammasome activation, which may contribute to its anti-inflammatory effects. Together, these findings show that Ori may be an effective candidate for ameliorating LPS-induced inflammatory responses.
authors.affiliation: Department of Hepatology, The First Hospital of Jilin University, Changchun 130021, China; Department of Nephrology, The First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Qingfei
authors.initials: Q
authors.lastname: Xiao
authors.affiliation: Department of Nephrology, The First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Zhihui
authors.identifier: 0000-0002-1960-6307
authors.initials: Z
authors.lastname: Qu
authors.affiliation: Department of Nephrology, The First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Nephrology, The First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Hepatology, The First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Pujun
authors.identifier: 0000-0002-9592-119X
authors.initials: P
authors.lastname: Gao