title: LW-AFC Effects onN-glycan Profile in Senescence-Accelerated Mouse Prone 8 Strain, a Mouse Model of Alzheimer's Disease.
abstract: Glycosylation is one of the most common eukaryotic post-translational modifications, and aberrant glycosylation has been linked to many diseases. However, glycosylation and glycome analysis is a significantly challenging task. Although several lines of evidence have indicated that protein glycosylation is defective in Alzheimer's disease (AD), only a few studies have focused on AD glycomics. The etiology of AD is unclear and there are no effective disease-modifying treatments for AD. In this study, we found that the object recognition memory, passive avoidance, and spatial learning and memory of senescence-accelerated mouse prone 8 (SAMP8) strain, an AD animal model, were deficient, and LW-AFC, which was prepared from the traditional Chinese medicine prescription Liuwei Dihuang decoction, showed beneficial effects on the deterioration of cognitive capability in SAMP8 mice. Forty-three and 56N-glycan were identified in the cerebral cortex and serum of SAMP8 mice, respectively. TheN-glycan profile in SAMP8 mice was significantly different from that of senescence accelerated mouse resistant 1 (SAMR1) strains, the control of SAMP8 mice. Treatment with LW-AFC modulated the abundance of 21 and 6N-glycan in the cerebral cortex and serum of SAMP8 mice, respectively. The abundance of (Hex)3(HexNAc)5(Fuc)1(Neu5Ac)1 and (Hex)2(HexNAc)4 decreased in the cerebral cortex and serum of SAMP8 mice compared with SAMR1 mice, decreases that were significantly correlated with learning and memory measures. The administration of LW-AFC could reverse or increase these levels in SAMP8 mice. These results indicated that the effects of LW-AFC on cognitive impairments in SAMP8 mice might be through modulation ofN-glycan patterns, and LW-AFC may be a potential anti-AD agent.
authors.affiliation: 1Department of TCM and Neuroimmunopharmacology, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China; 2State Key Laboratory of Toxicology and Medical Countermeasures, Beijing 100850, China.
authors.forename: Jianhui
authors.initials: J
authors.lastname: Wang
authors.affiliation: 1Department of TCM and Neuroimmunopharmacology, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China; 2State Key Laboratory of Toxicology and Medical Countermeasures, Beijing 100850, China.
authors.forename: Xiaorui
authors.initials: X
authors.lastname: Cheng
authors.affiliation: 1Department of TCM and Neuroimmunopharmacology, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China; 2State Key Laboratory of Toxicology and Medical Countermeasures, Beijing 100850, China.
authors.forename: Ju
authors.initials: J
authors.lastname: Zeng
authors.affiliation: 3Educational Ministry Key Laboratory of Resource Biology and Biotechnology in Western China, Life Sciences College, Northwest University, Xi'an 710069, China.
authors.forename: Jiangbei
authors.initials: J
authors.lastname: Yuan
authors.affiliation: 3Educational Ministry Key Laboratory of Resource Biology and Biotechnology in Western China, Life Sciences College, Northwest University, Xi'an 710069, China.
authors.forename: Zhongfu
authors.initials: Z
authors.lastname: Wang
authors.affiliation: 1Department of TCM and Neuroimmunopharmacology, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China; 2State Key Laboratory of Toxicology and Medical Countermeasures, Beijing 100850, China.
authors.forename: Wenxia
authors.initials: W
authors.lastname: Zhou
authors.affiliation: 1Department of TCM and Neuroimmunopharmacology, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China; 2State Key Laboratory of Toxicology and Medical Countermeasures, Beijing 100850, China.
authors.forename: Yongxiang
authors.initials: Y
authors.lastname: Zhang
keywords: Alzheimer’s disease; LW-AFC; glycome; senescence-accelerated mouse prone 8 strain; traditional Chinese medicine