title: Bladder Transplantation of Amniotic Fluid Stem Cell may Ameliorate Bladder Dysfunction After Focal Cerebral Ischemia in Rat.
abstract: The objective is to investigate whether human amniotic fluid stem cells (hAFSCs) grafting into the bladder may influence bladder functional and molecular changes in an animal stroke model. Female rats were divided into three groups: sham, middle cerebral artery occlusion (MCAO) alone, and MCAO plus 1 × 106hAFSCs transplanting into bladder wall. Bladder function was analyzed by cystometry at days 3 and 10 after MCAO. The expressions of bladder nerve growth factor (NGF), M2-muscarinic, M3-muscarinic, and P2X1 receptors were measured by immunohistochemistry and real-time polymerase chain reaction. When compared with sham-operated group, MCAO alone rats had significant increase in residual volume and decrease in voided volume and intercontraction interval; however, these bladder dysfunctions were improved following hAFSCs transplantation. The immunoreactivities of NGF, M3, and P2X1 significantly decreased at days 3 and 10, but M2 increased at day 3 after MCAO. Following hAFSCs transplantation, the immunoreactivities of NGF and P2X1 significantly increased at day 3, and M2 increased at day 10 after MCAO. The mRNAs of NGF, M2, and M3 significantly increased at day 3, but NGF and M2 decreased at day 10 after MCAO. Following hAFSCs transplantation, there was significant decrease in M2 mRNA at day 3 and increase in P2X1 mRNA at days 3 and 10 after MCAO. Bladder dysfunction caused by MCAO can be improved by hAFSCs transplanting into bladder which may be related to the expressions of bladder NGF, and muscarinic and P2X1 receptors. Stem Cells Translational Medicine 2017;6:1227-1236.
authors.affiliation: Female Urology Section, Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital Linkou Medical Center, Taoyuan, Taiwan, Republic of China.|College of Medicine, Chang Gung University, Taoyuan, Taiwan, Republic of China.
authors.forename: Ching-Chung
authors.initials: CC
authors.lastname: Liang
authors.affiliation: Division of Obstetrics, Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital Linkou Medical Center, Taoyuan, Taiwan, Republic of China.|College of Medicine, Chang Gung University, Taoyuan, Taiwan, Republic of China.|Prenatal Cell and Gene Therapy Group, Institute for Women's Health, University College London, London, United Kingdom.
authors.forename: S W Steven
authors.initials: SW
authors.lastname: Shaw
authors.affiliation: Female Urology Section, Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital Linkou Medical Center, Taoyuan, Taiwan, Republic of China.
authors.forename: Yung-Hsin
authors.initials: YH
authors.lastname: Huang
authors.affiliation: Female Urology Section, Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital Linkou Medical Center, Taoyuan, Taiwan, Republic of China.|College of Medicine, Chang Gung University, Taoyuan, Taiwan, Republic of China.
authors.forename: Yi-Hao
authors.initials: YH
authors.lastname: Lin
authors.affiliation: Stroke Center and Department of Neurology, Chang Gung Memorial Hospital Linkou Medical Center, Taoyuan, Taiwan, Republic of China.|College of Medicine, Chang Gung University, Taoyuan, Taiwan, Republic of China.
authors.forename: Tsong-Hai
authors.initials: TH
authors.lastname: Lee
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology*; D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism / Q000628:therapy*; D005260:Female; D007150:Immunohistochemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology*; D001743:Urinary Bladder / Q000166:cytology*
keywords: Amniotic fluid stem cell; Bladder; Cystometry; Middle cerebral artery occlusion; Stroke