title: Gcn5 determines the fate ofDrosophilagermline stem cells through degradation of Cyclin A.
abstract: The fluctuating CDK-CYCLIN complex plays a general role in cell-cycle control. Many types of stem cells use unique features of the cell cycle to facilitate asymmetric division. However, the manner in which these features are established remains poorly understood. The cell cycle ofDrosophilafemale germline stem cells (GSCs) is characterized by short G1and very long G2phases, making it an excellent model for the study of cell cycle control in stem cell fate determination. Using aDrosophilafemale GSC model, we found Gcn5, the first discovered histone acetyltransferase, to maintain germline stem cells inDrosophilaovaries. Results showed that Gcn5 is dispensable for the transcriptional silencing ofbam, but interacts with Cyclin A to facilitate proper turnover in GSCs. Results also showed that Gcn5 promotes Cyclin A ubiquitination, which is dependent on its acetylating activity. Finally, results showed that knockdown of Cyclin A rescued the GSC-loss phenotype caused by lack of Gcn5. Collectively, these findings support the conclusion that Gcn5 acts through acetylation to facilitate Cyclin A ubiquitination and proper turnover, thereby determining the fate of GSCs.-Liu, T., Wang, Q., Li, W., Mao, F., Yue, S., Liu, S., Liu, X., Xiao, S., Xia, L. Gcn5 determines the fate ofDrosophilagermline stem cells through degradation of Cyclin A.
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; and.|Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.
authors.forename: Tianqi
authors.initials: T
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; and.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; and.
authors.forename: Wenqing
authors.initials: W
authors.lastname: Li
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; and.
authors.forename: Feiyu
authors.initials: F
authors.lastname: Mao
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; and.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Yue
authors.affiliation: Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.
authors.forename: Sun
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.
authors.forename: Xiaona
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China asdfg@smu.edu.cn.
authors.forename: Shan
authors.initials: S
authors.lastname: Xiao
authors.affiliation: Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China xialx@smu.edu.cn.
authors.forename: Laixin
authors.initials: L
authors.lastname: Xia
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000502:physiology*; D002454:Cell Differentiation / Q000235:genetics*; D002455:Cell Division / Q000502:physiology; D019925:Cyclin A / Q000378:metabolism*; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster; D005260:Female; D005854:Germ Cells / Q000378:metabolism*; D051548:Histone Acetyltransferases / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism; D010641:Phenotype; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D000099224:p300-CBP-Associated Factor
chemical_list: D019925:Cyclin A; D029721:Drosophila Proteins; D051548:Histone Acetyltransferases; C470557:GCN5 protein, Drosophila; D000099224:p300-CBP-Associated Factor
keywords: cell cycle; development; gametogenesis; ubiquitination