title: Purification of quercetin-3-O-sophoroside and isoquercitrin from Poacynum hendersonii leaves using macroporous resins followed by Sephadex LH-20 column chromatography.
abstract: In China, Poacynum hendersonii is frequently used as a substitute for Apoacynum venetum L (Luobuma), which is a famous traditional Chinese medicine. Quercetin-3-O-sophoroside and isoquercitrin are two major flavonoids in Poacynum hendersonii leaves. In this work, a suitable method was established for the large-scale preparation of quercetin-3-O-sophoroside (QOS) and isoquercitrin (ISO) from Poacynum hendersonii leaves using macroporous resin combined with Sephadex LH-20 column chromatography. The adsorption/desorption capacities and desorption ratios of six macroporous resins were evaluated using static experiments. The HPD-300 resin had the best adsorption performance because it had the largest surface area, and was selected for further study. Compared with pseudo-first-order and intraparticle diffusion kinetics models, the pseudo-second-order model could better fit the adsorption kinetics of both QOS and ISO on the HPD-300 resin. In addition, the adsorption isotherms of the two compounds on the HPD-300 resin were fitted well to the Langmuir model. Under optimal conditions, the purities of QOS and ISO in the product were increased from 2.16% and 1.26% to 21.34% and 10.70% with recovery yields of 82.1% and 77.3%, respectively. Subsequently, Sephadex LH-20 column chromatography was employed for improving the purities of the two compounds. After separation by Sephadex LH-20 column chromatography, the purities of QOS and ISO achieved 93.5% and 95.6%, respectively.
authors.affiliation: Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China; School of Pharmacy, Binzhou Medical University, Yantai 264003, PR China; University of Chinese Academy of Sciences,19 Yuquan Road, Beijing 100049, PR China.
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Resources and Environmental Engineering, Ludong University, Yantai 264025, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China.
authors.forename: Yuetao
authors.initials: Y
authors.lastname: Yi
authors.affiliation: Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China; University of Chinese Academy of Sciences,19 Yuquan Road, Beijing 100049, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Miao
authors.affiliation: Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China.
authors.forename: Sujing
authors.initials: S
authors.lastname: Liu
authors.affiliation: School of Pharmacy, Binzhou Medical University, Yantai 264003, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.affiliation: School of Pharmacy, Binzhou Medical University, Yantai 264003, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cong
authors.affiliation: School of Pharmacy, Binzhou Medical University, Yantai 264003, PR China. Electronic address: chunhuawang508@126.com.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Wang
authors.affiliation: School of Resources and Environmental Engineering, Ludong University, Yantai 264025, China. Electronic address: chxia_ldu@hotmail.com.
authors.forename: Chuanhai
authors.initials: C
authors.lastname: Xia
mesh_terms: D000327:Adsorption; D030010:Apocynum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003911:Dextrans / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D012117:Resins, Synthetic / Q000737:chemistry
chemical_list: D003911:Dextrans; D010936:Plant Extracts; D012117:Resins, Synthetic; C016527:isoquercitrin; C055545:quercetin-3-O-sophoroside; C025614:sephadex; D011794:Quercetin
keywords: Isoquercitrin; Macroporous resins; Poacynum hendersonii; Purification; Quercetin-3-O-sophoroside