title: Green tea polyphenol tailors cell adhesivity of RGD displaying surfaces: multicomponent models monitored optically.
abstract: The interaction of the anti-adhesive coating, poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) and its Arg-Gly-Asp (RGD) functionalized form, PLL-g-PEG-RGD, with the green tea polyphenol, epigallocatechin-gallate (EGCg) was in situ monitored. After, the kinetics of cellular adhesion on the EGCg exposed coatings were recorded in real-time. The employed plate-based waveguide biosensor is applicable to monitor small molecule binding and sensitive to sub-nanometer scale changes in cell membrane position and cell mass distribution; while detecting the signals of thousands of adhering cells. The combination of this remarkable sensitivity and throughput opens up new avenues in testing complicated models of cell-surface interactions. The systematic studies revealed that, despite the reported excellent antifouling properties of the coatings, EGCg strongly interacted with them, and affected their cell adhesivity in a concentration dependent manner. Moreover, the differences between the effects of the fresh and oxidized EGCg solutions were first demonstrated. Using a semiempirical quantumchemical method we showed that EGCg binds to the PEG chains of PLL-g-PEG-RGD and effectively blocks the RGD sites by hydrogen bonds. The calculations supported the experimental finding that the binding is stronger for the oxidative products. Our work lead to a new model of polyphenol action on cell adhesion ligand accessibility and matrix rigidity.
authors.affiliation: Doctoral School of Molecular and Nanotechnologies, Faculty of Information Technology, University of Pannonia, H-8200 Egyetem u. 10, Veszprém, Hungary.|Nanobiosensorics Group, Hungarian Academy of Sciences, Research Centre for Natural Sciences, Institute for Technical Physics and Materials Science, Konkoly-Thege u, 29-33, H-1120 Budapest, Hungary.
authors.forename: Beatrix
authors.initials: B
authors.lastname: Peter
authors.affiliation: Nanobiosensorics Group, Hungarian Academy of Sciences, Research Centre for Natural Sciences, Institute for Technical Physics and Materials Science, Konkoly-Thege u, 29-33, H-1120 Budapest, Hungary.|Chemical Engineering and Material Science Doctoral School, University of Pannonia, H-8200 Egyetem u, 10, Veszprém, Hungary.
authors.forename: Eniko
authors.initials: E
authors.lastname: Farkas
authors.affiliation: Nanobiosensorics Group, Hungarian Academy of Sciences, Research Centre for Natural Sciences, Institute for Technical Physics and Materials Science, Konkoly-Thege u, 29-33, H-1120 Budapest, Hungary.
authors.forename: Eniko
authors.initials: E
authors.lastname: Forgacs
authors.affiliation: Nanobiosensorics Group, Hungarian Academy of Sciences, Research Centre for Natural Sciences, Institute for Technical Physics and Materials Science, Konkoly-Thege u, 29-33, H-1120 Budapest, Hungary.|George Olah Doctoral School, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest 1111, Hungary.
authors.forename: Andras
authors.initials: A
authors.lastname: Saftics
authors.affiliation: Nanobiosensorics Group, Hungarian Academy of Sciences, Research Centre for Natural Sciences, Institute for Technical Physics and Materials Science, Konkoly-Thege u, 29-33, H-1120 Budapest, Hungary.|Chemical Engineering and Material Science Doctoral School, University of Pannonia, H-8200 Egyetem u, 10, Veszprém, Hungary.
authors.forename: Boglarka
authors.initials: B
authors.lastname: Kovacs
authors.affiliation: Nanobiosensorics Group, Hungarian Academy of Sciences, Research Centre for Natural Sciences, Institute for Technical Physics and Materials Science, Konkoly-Thege u, 29-33, H-1120 Budapest, Hungary.
authors.forename: Sandor
authors.initials: S
authors.lastname: Kurunczi
authors.affiliation: Nanobiosensorics Group, Hungarian Academy of Sciences, Research Centre for Natural Sciences, Institute for Technical Physics and Materials Science, Konkoly-Thege u, 29-33, H-1120 Budapest, Hungary.
authors.forename: Inna
authors.initials: I
authors.lastname: Szekacs
authors.affiliation: Institute of Chemistry, Eötvös Loránd University, Budapest 112, POB 32, H-1518, Hungary.
authors.forename: Antal
authors.initials: A
authors.lastname: Csampai
authors.affiliation: MTA-ELTE Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Eötvös Loránd University, Budapest 112, POB 32, H-1518, Hungary.
authors.forename: Szilvia
authors.initials: S
authors.lastname: Bosze
authors.affiliation: Nanobiosensorics Group, Hungarian Academy of Sciences, Research Centre for Natural Sciences, Institute for Technical Physics and Materials Science, Konkoly-Thege u, 29-33, H-1120 Budapest, Hungary.
authors.forename: Robert
authors.initials: R
authors.lastname: Horvath
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D019281:Dimerization; D006367:HeLa Cells; D006801:Humans; D006860:Hydrogen Bonding; D007700:Kinetics; D008954:Models, Biological; D008958:Models, Molecular; D009842:Oligopeptides / Q000494:pharmacology*; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry; D059808:Polyphenols / Q000494:pharmacology*; D013057:Spectrum Analysis; D013499:Surface Properties; D013662:Tea / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D009842:Oligopeptides; D011108:Polymers; D059808:Polyphenols; D013662:Tea; C047981:arginyl-glycyl-aspartic acid; D002392:Catechin; C045651:epigallocatechin gallate