title: AtSKIP18 and AtSKIP31, F-box subunits of the SCF E3 ubiquitin ligase complex, mediate the degradation of 14-3-3 proteins in Arabidopsis.
abstract: 14-3-3 proteins regulate numerous cellular processes through interaction with their target proteins in a phosphorylation dependent manner. Although proteins that are regulated by 14-3-3s have been studied, the regulatory mechanism of 14-3-3s is poorly understood. In the present study, F-box proteins, a component of Skp1-Cullin-F-box E3 ubiquitin ligase, were identified as 14-3-3 targets using yeast two-hybrid screening. Among them, AtSKIP18 and AtSKIP31, were shown to mediate the degradation of Arabidopsis 14-3-3s. Mutational analyses of AtSKIP18 and AtSKIP31 indicated that the phosphorylation of AtSKIPs is critical for interaction and degradation of 14-3-3s. The loss-of-function mutation in AtSKIP31 resulted in enhanced primary root growth under nitrogen deficient conditions. These findings suggest that AtSKIP31 regulates the primary root growth in nitrogen deficiency via degrading 14-3-3s.
authors.affiliation: RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Jong-Pil
authors.initials: JP
authors.lastname: Hong
authors.affiliation: RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Adams
authors.affiliation: RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan; Institute of Agrobiological Sciences, NARO, Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Yanagawa
authors.affiliation: RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Minami
authors.initials: M
authors.lastname: Matsui
authors.affiliation: RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan. Electronic address: ryoung.shin@riken.jp.
authors.forename: Ryoung
authors.initials: R
authors.lastname: Shin
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism*; D017360:Arabidopsis / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D044783:F-Box Proteins / Q000378:metabolism*; D010766:Phosphorylation; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism; D060066:Protein Interaction Maps; D059748:Proteolysis; D044843:SKP Cullin F-Box Protein Ligases / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: D048948:14-3-3 Proteins; D029681:Arabidopsis Proteins; D044783:F-Box Proteins; C000619941:SKIP18 protein, Arabidopsis; C000619942:SKIP31 protein, Arabidopsis; D044843:SKP Cullin F-Box Protein Ligases; D044767:Ubiquitin-Protein Ligases
keywords: 14-3-3 protein; F-box; Nitrogen; SCF E3 ubiquitin ligase