title: Comparative RNA-Seq analysis of differentially expressed genes in the testis and ovary of Takifugu rubripes.
abstract: Takifugu rubripes is a classical model organism for studying the role of gonad organogenesis in such physiological processes as fertilization, sex determination, and sexual development. To explicitly investigate the mechanism associated with gonad organogenesis in T. rubripes, we obtained 44.3 million and 55.2 million raw reads from the testis and ovary, respectively, by RNA-seq and from these, 18,523 genes were identified. A total of 680 differentially expressed genes were obtained from comparison transcriptome analysis between the testis and ovary, and of these, 556 genes were up-regulated in the testis, whereas only 124 genes were upregulated in the ovary. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that many of these genes encode proteins involved in signal transduction and gonad development. We mainly focused on the differentially expressed genes that have the potential to develop into the gonad. The generation of large scale transcriptomic data presented in this work would enrich the genetic resources of T. rubripes, which should be valuable to the comparative and evolutionary studies of teleosts.
authors.affiliation: College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
authors.forename: Zhicheng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China. Electronic address: xmqiu@dlou.edu.cn.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Qiu
authors.affiliation: College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
authors.forename: Derong
authors.initials: D
authors.lastname: Kong
authors.affiliation: College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Guangxi Academy of Fishery Sciences, Nanning 530021, China.
authors.forename: Zhongbao
authors.initials: Z
authors.lastname: Guo
authors.affiliation: College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
authors.forename: Changfu
authors.initials: C
authors.lastname: Gao
authors.affiliation: College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Ma
authors.affiliation: College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Hao
authors.affiliation: College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Dalian Tianzheng Industrial Corporation Limited, Dalian 116011, China.
authors.forename: Shengcong
authors.initials: S
authors.lastname: Liu
authors.affiliation: Dalian Tianzheng Industrial Corporation Limited, Dalian 116011, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Dalian Tianzheng Industrial Corporation Limited, Dalian 116011, China.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Meng
authors.affiliation: College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China. Electronic address: xiuliwang417@sina.com.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D005260:Female; D005786:Gene Expression Regulation; D063990:Gene Ontology; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D008297:Male; D058977:Molecular Sequence Annotation; D010053:Ovary / Q000166:cytology / Q000378:metabolism*; D023741:Takifugu / Q000235:genetics* / Q000254:growth & development; D013737:Testis / Q000166:cytology / Q000378:metabolism*; D059467:Transcriptome / Q000235:genetics*
keywords: Differentially expressed genes; Ovary tissue; Takifugu rubripes; Testis tissue; Transcriptome analysis