title: Strong Correlation among Three Biodosimetry Techniques Following Exposures to Ionizing Radiation.
abstract: Threein vitrodose calibration curves for biodosimetry such as dicentric chromosome assay, fluorescencein situhybridization (FISH) assay for translocation, and micronuclei (MNs) in binucleated cell assay were established after exposure to ionizing radiation. Peripheral blood lymphocyte samples obtained from healthy donors were irradiated with60Co source at a dose rate of 0.5 Gy/min to doses of 0.1-6 Gy. The results from threein vitrodose calibration curves for biodosimetry were analyzed to understand the relationship among biodosimetry assay techniques. Our comparison demonstrates that there is a very strong positive correlation among the dicentric assay, FISH, and MNs analysis, and these three biodosimetry assays strongly support thein vitrodose reconstruction and the emergency preparedness of public or occupational radiation overexposure.
authors.affiliation: Division of Radiation Effect, Korea Institute of Radiological and Medical Sciences, Seoul 01812, Republic of Korea.
authors.forename: Chang-Mo
authors.initials: CM
authors.lastname: Kang
authors.affiliation: Division of Radiation Effect, Korea Institute of Radiological and Medical Sciences, Seoul 01812, Republic of Korea.
authors.forename: Hyun Jin
authors.initials: HJ
authors.lastname: Yun
authors.affiliation: Radiation Epidemiology Team, Radiation Health Institute, Korea Hydro and Nuclear Power Co., Gyeonggi-do 13605, Republic of Korea.
authors.forename: Hanna
authors.initials: H
authors.lastname: Kim
authors.affiliation: Radiation Epidemiology Team, Radiation Health Institute, Korea Hydro and Nuclear Power Co., Gyeonggi-do 13605, Republic of Korea.
authors.forename: Cha Soon
authors.initials: CS
authors.lastname: Kim
keywords: Dicentric assay; fluorescence in situ hybridization; ionizing radiation; micronucleus assay; translocations