title: Characterization and cytotoxic effect of aqua-(2,2',2''-nitrilotriacetato)-oxo-vanadium salts on human osteosarcoma cells.
abstract: The use of protonated N-heterocyclic compound, i.e. 2,2'-bipyridinium cation, [bpyH+], enabled to obtain the new nitrilotriacetate oxidovanadium(IV) salt of the stoichiometry [bpyH][VO(nta)(H2O)]H2O. The X-ray measurements have revealed that the compound comprises the discrete mononuclear [VO(nta)(H2O)]-coordination ion that can be rarely found among other known compounds containing nitrilotriacetate oxidovanadium(IV) moieties. The antitumor activity of [bpyH][VO(nta)(H2O)]H2O and its phenanthroline analogue, [phenH][VO(nta)(H2O)](H2O)0.5, towards human osteosarcoma cell lines (MG-63 and HOS) has been assessed (the LDH and BrdU tests) and referred to cis-Pt(NH3)2Cl2(used as a positive control). The compounds exert a stronger cytotoxic effect on MG-63 and HOS cells than in untransformed human osteoblast cell line. Thus, the [VO(nta)(H2O)]-containing coordination compounds can be considered as possible antitumor agents in the osteosarcoma model of bone-related cells in culture.
authors.affiliation: Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308, Gdańsk, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Tesmar
authors.affiliation: Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308, Gdańsk, Poland. dariusz.wyrzykowski@ug.edu.pl.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Wyrzykowski
authors.affiliation: Institute of General and Ecological Chemistry, Technical University of Łódź, Żwirki 36, 90-924, Łódź, Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Kruszyński
authors.affiliation: Department of Medical Chemistry, Medical University of Gdańsk, Dębinki 1, 80-211, Gdańsk, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Niska
authors.affiliation: Department of Medical Chemistry, Medical University of Gdańsk, Dębinki 1, 80-211, Gdańsk, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Inkielewicz-Stępniak
authors.affiliation: Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308, Gdańsk, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Drzeżdżon
authors.affiliation: Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308, Gdańsk, Poland.
authors.forename: Dagmara
authors.initials: D
authors.lastname: Jacewicz
authors.affiliation: Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308, Gdańsk, Poland.
authors.forename: Lech
authors.initials: L
authors.lastname: Chmurzyński
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D056831:Coordination Complexes / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives / Q000494:pharmacology*; D010006:Osteoblasts / Q000187:drug effects* / Q000473:pathology; D011199:Potentiometry; D017968:Vanadium Compounds / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes; D017968:Vanadium Compounds; D009571:Nitrilotriacetic Acid
keywords: Antitumor activity; Crystal structure; Osteosarcoma cells; Potentiometric titration; Vanadium