title: Antimicrobial efficiency of PAA/(PVP/CHI) erodible polysaccharide multilayer through loading and controlled release of antibiotics.
abstract: The adhesion of bacteria and subsequent formation of biofilm on the surface of implants greatly affect the long-term use of the implants. The low molar mass gentamicin (GS) cations could hardly be directly incorporated into the multilayer films through alternately deposition with a polyanion. Herein, we have designed and constructed a (poly(acrylic acid)/(polyvinylpyrrolidone/chitosan))n((PAA/(PVP/CHI))n) multilayer films through layer-by-layer self-assembly method. Through increasing the pH to destroy hydrogen bonding between PAA and PVP, PVP released into the solution and GS simultaneously combined with PAA through electrostatic interactions. The loading dosage of GS into the (PAA/(PVP/CHI))10multilayer film was up to 153.84±18.64μg/cm2and could be precisely tuned through changing the thickness of the films. The release behaviour of GS in phosphate buffer saline could also be regulated through thermal cross-linking of the films. The drug-loaded multilayer films displayed efficient against three kinds of Gram-positive and three kinds of Gram-negative bacteria and one kind of fungi, and good biocompatibility towards human lens epithelial cells. GS-loaded multilayer films-coated polydimethylsiloxane (PDMS) were compared with pristine PDMS in the rabbit subcutaneous S. aureus infection model. The antimicrobial-coated implants yielded a much lower degree of infections than pristine implants at day seven.
authors.affiliation: School of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, 325027, China.
authors.forename: Qingwen
authors.initials: Q
authors.lastname: Xu
authors.affiliation: School of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, 325027, China.
authors.forename: Huihua
authors.initials: H
authors.lastname: Liu
authors.affiliation: School of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, 325027, China.
authors.forename: Zi
authors.initials: Z
authors.lastname: Ye
authors.affiliation: School of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, 325027, China.
authors.forename: Kaihui
authors.initials: K
authors.lastname: Nan
authors.affiliation: School of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, 325027, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Lin
authors.affiliation: School of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, 325027, China; Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences, Wenzhou, 32500, China. Electronic address: Chenhao823@mail.eye.ac.cn.
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.affiliation: School of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, 325027, China; Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences, Wenzhou, 32500, China. Electronic address: wangbailiang2006@aliyun.com.
authors.forename: Bailiang
authors.initials: B
authors.lastname: Wang
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001419:Bacteria / Q000187:drug effects*; D003692:Delayed-Action Preparations / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D004847:Epithelial Cells / Q000187:drug effects; D006801:Humans; D011134:Polysaccharides / Q000494:pharmacology*; D011817:Rabbits; D013203:Staphylococcal Infections / Q000188:drug therapy
chemical_list: D000890:Anti-Infective Agents; D003692:Delayed-Action Preparations; D011134:Polysaccharides
keywords: Antibacterial; Drug delivery; Hydrogen-bonding; Layer-by-layer self-assembly; Thermal cross-linking