title: Complexes of Magnetic Nanoparticles with Cellulose Nanocrystals as Regenerable, Highly Efficient, and Selective Platform for Protein Separation.
abstract: We present an efficient approach to develop cellulose nanocrystal (CNC) hybrids with magnetically responsive Fe3O4nanoparticles that were synthesized using the (Fe3+/Fe2+) coprecipitation. After 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-catalyzed oxidation of CNC, carbodiimide (EDC/NHS) was used for coupling amine-containing iron oxide nanoparticles that were achieved by dopamine ligand exchange (NH2-Fe3O4NPs). The as-prepared hybrids (Fe3O4@CNC) were further complexed with Cu(II) ions to produce specific protein binding sites. The performance of magnetically responsive Cu-Fe3O4@CNC hybrids was assessed by selectively separating lysozyme from aqueous media. The hybrid system displayed a remarkable binding capacity with lysozyme of 860.6 ± 14.6 mg/g while near full protein recovery (∼98%) was achieved by simple elution. Moreover, the regeneration of Fe3O4@CNC hybrids and efficient reutilization for protein separation was demonstrated. Finally, lysozyme separation from matrices containing egg white was achieved, thus revealing the specificity and potential of the presented method.
authors.affiliation: Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University , FI-00076 Aalto, Finland.
authors.forename: Jiaqi
authors.identifier: 0000-0001-7557-0356
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University , FI-00076 Aalto, Finland.|Alabama Center for Paper and Bioresource Engineering, Department of Chemical Engineering, Auburn University , Auburn, Alabama 36849-5127, United States.
authors.forename: Ilari
authors.identifier: 0000-0003-0538-6523
authors.initials: I
authors.lastname: Filpponen
authors.affiliation: Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University , FI-00076 Aalto, Finland.
authors.forename: Leena-Sisko
authors.initials: LS
authors.lastname: Johansson
authors.affiliation: Alabama Center for Paper and Bioresource Engineering, Department of Chemical Engineering, Auburn University , Auburn, Alabama 36849-5127, United States.
authors.forename: Pezhman
authors.initials: P
authors.lastname: Mohammadi
authors.affiliation: Department of Applied Physics, School of Science, Aalto University , FI-00076 Aalto, Finland.
authors.forename: Mika
authors.initials: M
authors.lastname: Latikka
authors.affiliation: Alabama Center for Paper and Bioresource Engineering, Department of Chemical Engineering, Auburn University , Auburn, Alabama 36849-5127, United States.
authors.forename: Markus B
authors.initials: MB
authors.lastname: Linder
authors.affiliation: Department of Applied Physics, School of Science, Aalto University , FI-00076 Aalto, Finland.
authors.forename: Robin H A
authors.initials: RH
authors.lastname: Ras
authors.affiliation: Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University , FI-00076 Aalto, Finland.|Department of Applied Physics, School of Science, Aalto University , FI-00076 Aalto, Finland.|Departments of Forest Biomaterials and Chemical and Biomolecular Engineering, North Carolina State University , Raleigh, North Carolina 27695, United States.
authors.forename: Orlando J
authors.identifier: 0000-0003-4036-4020
authors.initials: OJ
authors.lastname: Rojas
mesh_terms: D002384:Catalysis; D002482:Cellulose / Q000737:chemistry*; D003497:Cyclic N-Oxides / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D009113:Muramidase / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D011506:Proteins / Q000737:chemistry*
chemical_list: D003497:Cyclic N-Oxides; D058185:Magnetite Nanoparticles; D011506:Proteins; D002482:Cellulose; D009113:Muramidase; C003959:TEMPO