title: Subependymal Zone-Derived Oligodendroblasts Respond to Focal Demyelination but Fail to Generate Myelin in Young and Aged Mice.
abstract: Two populations of oligodendrogenic progenitors co-exist within the corpus callosum (CC) of the adult mouse. Local, parenchymal oligodendrocyte progenitor cells (pOPCs) and progenitors generated in the subependymal zone (SEZ) cytogenic niche. pOPCs are committed perinatally and retain their numbers through self-renewing divisions, while SEZ-derived cells are relatively "young," being constantly born from neural stem cells. We compared the behavior of these populations, labeling SEZ-derived cells using hGFAP:CreErt2mice, within the homeostatic and regenerating CC of the young-adult and aging brain. We found that SEZ-derived oligodendroglial progenitors have limited self-renewing potential and are therefore not bona fide OPCs but rather "oligodendroblasts" more similar to the neuroblasts of the neurogenic output of the SEZ. In the aged CC their mitotic activity is much reduced, although they still act as a "fast-response element" to focal demyelination. In contrast to pOPCs, they fail to generate mature myelinating oligodendrocytes at all ages studied.
authors.affiliation: Wellcome Trust-MRC Cambridge Stem Cell Biology Institute, University of Cambridge, Cambridge CB2 0AH, UK; Lab of Developmental Biology, Department of Biology, University of Patras, Patras 26500, Greece. Electronic address: ikazanis@upatras.gr.
authors.forename: Ilias
authors.initials: I
authors.lastname: Kazanis
authors.affiliation: Wellcome Trust-MRC Cambridge Stem Cell Biology Institute, University of Cambridge, Cambridge CB2 0AH, UK.
authors.forename: Kimberley A
authors.initials: KA
authors.lastname: Evans
authors.affiliation: Lab of Developmental Biology, Department of Biology, University of Patras, Patras 26500, Greece.
authors.forename: Evangelia
authors.initials: E
authors.lastname: Andreopoulou
authors.affiliation: Lab of Developmental Biology, Department of Biology, University of Patras, Patras 26500, Greece.
authors.forename: Christina
authors.initials: C
authors.lastname: Dimitriou
authors.affiliation: Lab of Developmental Biology, Department of Biology, University of Patras, Patras 26500, Greece.
authors.forename: Christos
authors.initials: C
authors.lastname: Koutsakis
authors.affiliation: Wellcome Trust-MRC Cambridge Stem Cell Biology Institute, University of Cambridge, Cambridge CB2 0AH, UK.
authors.forename: Ragnhildur Thora
authors.initials: RT
authors.lastname: Karadottir
authors.affiliation: Wellcome Trust-MRC Cambridge Stem Cell Biology Institute, University of Cambridge, Cambridge CB2 0AH, UK. Electronic address: rjf1000@cam.ac.uk.
authors.forename: Robin J M
authors.initials: RJM
authors.lastname: Franklin
mesh_terms: D000367:Age Factors; D000818:Animals; D015415:Biomarkers; D001921:Brain / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation; D003711:Demyelinating Diseases / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D051379:Mice; D008822:Mice, Transgenic; D009186:Myelin Sheath / Q000378:metabolism*; D055495:Neurogenesis; D009836:Oligodendroglia / Q000166:cytology* / Q000378:metabolism*; D055153:Stem Cell Niche
chemical_list: D015415:Biomarkers
keywords: OPC; ageing; corpus callosum; demyelination; myelin; myelination; neural stem cell; oligodendroblast; oligodendrocyte progenitor cell; remyelination; subependymal zone; subventricular zone