title: Evaluation of two novel chemiluminescence immunoassays for the detection of Clostridium difficile glutamate dehydrogenase and toxin A&B.
abstract: A novel immunoassay for Clostridium difficile glutamate dehydrogenase (GDH) and toxin A&B (LIAISON, DiaSorin) was compared to another GDH assay (Alere), PCR and toxigenic culture. The GDH-DiaSorin is slightly more sensitive than the GDH-Alere. Sensitivity of the Toxin-Diasorin test is in accordance to the sensitivity of other immunoassays in literature.
authors.affiliation: Division of Clinical Microbiology, University Hospital Basel, Basel, Switzerland. Electronic address: annette.blaich@usb.ch.
authors.forename: Annette
authors.initials: A
authors.lastname: Blaich
authors.affiliation: Division of Clinical Microbiology, University Hospital Basel, Basel, Switzerland.
authors.forename: Reno
authors.initials: R
authors.lastname: Frei
authors.affiliation: Division of Clinical Microbiology, University Hospital Basel, Basel, Switzerland.
authors.forename: Carine
authors.initials: C
authors.lastname: Castellano
authors.affiliation: Division of Clinical Microbiology, University Hospital Basel, Basel, Switzerland.
authors.forename: Christine
authors.initials: C
authors.lastname: Kiessling
authors.affiliation: Division of Clinical Chemistry, University Hospital Basel, Basel, Switzerland.
authors.forename: Angelika
authors.initials: A
authors.lastname: Geschke
authors.affiliation: Division of Clinical Chemistry, University Hospital Basel, Basel, Switzerland.
authors.forename: Katharina M
authors.initials: KM
authors.lastname: Rentsch
authors.affiliation: Division of Clinical Microbiology, University Hospital Basel, Basel, Switzerland.
authors.forename: Adrian
authors.initials: A
authors.lastname: Egli
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification*; D001427:Bacterial Toxins / Q000235:genetics / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods; D016360:Clostridioides difficile / Q000201:enzymology* / Q000235:genetics*; D004768:Enterotoxins / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000737:chemistry / Q000382:microbiology; D005969:Glutamate Dehydrogenase / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation / Q000379:methods*; D049449:Luminescence; D016133:Polymerase Chain Reaction / Q000379:methods; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004768:Enterotoxins; C041348:tcdA protein, Clostridium difficile; C031815:toxB protein, Clostridium difficile; D005969:Glutamate Dehydrogenase
keywords: Clostridium difficile; Glutamate dehydrogenase; Toxins A&B