title: Cytogenotoxicological defense of retinyl palmitate in the front damage of antineoplastics.
abstract: Cancer, the multifactorial pathology and to date is the most lethal causes of death in the world. Cyclophosphamide (CPA) and doxorubicin (DOX) are the individually or combindly used two anticancer drugs. The antineoplastic drugs-mediated genetic instability can be overcome by using antioxidants. The study evaluated the cytogenotoxic modulatory potentials of retinyl palmitate (RP) caused by CPA and DOX in Swiss mice. For this, adult Mus musculus of either sex were divided equally regarding to the gender. Toxicogenetic effects were induced by the intraperitoneal (i.p.) administration of the CPA (20mg/kg) and/or DOX (2mg/kg), following to test for comet assay and micronucleus test in bone marrow cells after 48h (DOX) and 7h (CPA) of the administration of RP (100 IU/kg). Both CPA and DOX significantly (p<0.05) increased with the index and frequency of damages, clastogenic and/or aneugenic effects with the augmenting of micronuclei, demonstrating the cytotoxicity interference on the ratio of normochromatic to polychromatic erythrocytes and bone marrow cells of mice, that were found to reduce in RP treatment groups. In conclusion, RP has a modulatory effect on CPA and DOX-mediated cytogenotoxic events. The findings may be a good indication to manage the antioneoplastic drug-induced stress mediated detrimental effects by using RP, especially as a side effect minimizer.
authors.affiliation: Laboratory of Toxicology and Genetics, Federal University of Piauí, Teresina, Piaui, 64.049-550, Brazil.
authors.forename: Ricardo Melo
authors.initials: RM
authors.lastname: de Carvalho
authors.affiliation: Laboratory of Toxicology and Genetics, Federal University of Piauí, Teresina, Piaui, 64.049-550, Brazil.
authors.forename: Rai Pablo de Sousa
authors.initials: RPS
authors.lastname: Aguiar
authors.affiliation: Laboratory of Toxicology and Genetics, Federal University of Piauí, Teresina, Piaui, 64.049-550, Brazil; Postgraduate Program in Biotechnology (RENORBIO), Federal University of Piauí, Teresina, Piauí, 64.049-550, Brazil; Department of Pharmacy, Southern University Bangladesh, Mehedibag, Chittagong, 4000, Bangladesh. Electronic address: rbiotufpi.br@gmail.com.
authors.forename: Muhammad Torequl
authors.initials: MT
authors.lastname: Islam
authors.affiliation: Laboratory of Toxicology and Genetics, Federal University of Piauí, Teresina, Piaui, 64.049-550, Brazil; Postgraduate Program in Biotechnology (RENORBIO), Federal University of Piauí, Teresina, Piauí, 64.049-550, Brazil.
authors.forename: Marcus Vinicius Oliveira Barros
authors.initials: MVOB
authors.lastname: de Alencar
authors.affiliation: Laboratory of Toxicology and Genetics, Federal University of Piauí, Teresina, Piaui, 64.049-550, Brazil.
authors.forename: Ana Maria Oliveira Ferreira
authors.initials: AMOF
authors.lastname: da Mata
authors.affiliation: Laboratory of Toxicology and Genetics, Federal University of Piauí, Teresina, Piaui, 64.049-550, Brazil.
authors.forename: Antonio Lima
authors.initials: AL
authors.lastname: Braga
authors.affiliation: Postgraduate Program in Biotechnology (RENORBIO), Federal University of Piauí, Teresina, Piauí, 64.049-550, Brazil.
authors.forename: Josemar José da Silva
authors.initials: JJDS
authors.lastname: Júnior
authors.affiliation: Laboratory of Toxicology and Genetics, Federal University of Piauí, Teresina, Piaui, 64.049-550, Brazil.
authors.forename: Leonardo da Rocha
authors.initials: LDR
authors.lastname: Sousa
authors.affiliation: Laboratory of Toxicology and Genetics, Federal University of Piauí, Teresina, Piaui, 64.049-550, Brazil; Postgraduate Program in Biotechnology (RENORBIO), Federal University of Piauí, Teresina, Piauí, 64.049-550, Brazil.
authors.forename: Rosália Maria Tôrres
authors.initials: RMT
authors.lastname: de Lima
authors.affiliation: Laboratory of Toxicology and Genetics, Federal University of Piauí, Teresina, Piaui, 64.049-550, Brazil; Postgraduate Program in Biotechnology (RENORBIO), Federal University of Piauí, Teresina, Piauí, 64.049-550, Brazil.
authors.forename: Márcia Fernanda Correia Jardim
authors.initials: MFCJ
authors.lastname: Paz
authors.affiliation: Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina, Piauí, 64.049-550, Brazil; Department of Biological Sciences, Federal University of Piauí, Picos, Piauí, 64.607-670, Brazil.
authors.forename: João Marcelo de Castro
authors.initials: JMC
authors.lastname: E Sousa
authors.affiliation: Laboratory of Toxicology and Genetics, Federal University of Piauí, Teresina, Piaui, 64.049-550, Brazil; Postgraduate Program in Biotechnology (RENORBIO), Federal University of Piauí, Teresina, Piauí, 64.049-550, Brazil; Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina, Piauí, 64.049-550, Brazil.
authors.forename: Ana Amélia de Carvalho
authors.initials: AAC
authors.lastname: Melo-Cavalcante
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D000975:Antioxidants / Q000494:pharmacology*; D003520:Cyclophosphamide / Q000633:toxicity*; D004249:DNA Damage / Q000187:drug effects*; D004224:Diterpenes; D004317:Doxorubicin / Q000633:toxicity*; D005260:Female; D008297:Male; D051379:Mice; D000084562:Retinyl Esters; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D004224:Diterpenes; D000084562:Retinyl Esters; D014801:Vitamin A; C014794:retinol palmitate; D004317:Doxorubicin; D003520:Cyclophosphamide
keywords: Cyclophosphamide; Doxorubicin; Retinyl palmitate; Toxicogenetic effects