title: Purification and Partial Structural Characterization of a Complement Fixating Polysaccharide from Rhizomes of Ligusticum chuanxiong.
abstract: Rhizome ofLigusticum chuanxiongis an effective medical plant, which has been extensively applied for centuries in migraine and cardiovascular diseases treatment in China. Polysaccharides from this plant have been shown to have interesting bioactivities, but previous studies have only been performed on the neutral polysaccharides. In this study, LCP-I-I, a pectic polysaccharide fraction, was obtained from the 100 °C water extracts ofL. chuangxiongrhizomes and purified by diethylaminethyl (DEAE) sepharose anion exchange chromatography and gel filtration. Monosaccharide analysis and linkage determination in addition to Fourier transform infrared (FT-IR) spectrometer and Nuclear magnetic resonance (NMR) spectrum, indicated that LCP-I-I is a typical pectic polysaccharide, with homo-galacturonan and rhamnogalacturonan type I regions and arabinogalactan type I and type II (AG-I/AG-II) side chains. LCP-I-I exhibited potent complement fixation activity, ICH50of 26.3 ± 2.2 µg/mL, and thus has potential as a natural immunomodulator.
authors.affiliation: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China. yuanfengzou@sicau.edu.cn.
authors.forename: Yuan-Feng
authors.initials: YF
authors.lastname: Zou
authors.affiliation: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China. yupingfu424@163.com.
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Fu
authors.affiliation: Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, College of Agronomy, Sichuan Agricultural University, Wenjiang 611130, China. chenxf64@sohu.com.
authors.forename: Xing-Fu
authors.initials: XF
authors.lastname: Chen
authors.affiliation: Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern 0316 Oslo, Norway. ingvild.austarheim@gmail.com.
authors.forename: Ingvild
authors.initials: I
authors.lastname: Austarheim
authors.affiliation: Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern 0316 Oslo, Norway. k.t.inngjerdingen@farmasi.uio.no.
authors.forename: Kari Tvete
authors.initials: KT
authors.lastname: Inngjerdingen
authors.affiliation: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China. huangchao@sicau.edu.cn.
authors.forename: Chao
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern 0316 Oslo, Norway. lemlemde@student.farmasi.uio.no.
authors.forename: Lemlem Dugassa
authors.initials: LD
authors.lastname: Eticha
authors.affiliation: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China. tonysong838@aliyun.com.
authors.forename: Xu
authors.initials: X
authors.lastname: Song
authors.affiliation: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China. lilixia905@163.com.
authors.forename: Lixia
authors.initials: L
authors.lastname: Li
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, Wenjiang 611130, China. fengbin@sicau.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Feng
authors.affiliation: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China. lorri190@126.com.
authors.forename: Chang-Liang
authors.initials: CL
authors.lastname: He
authors.affiliation: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China. yinzhongq@163.com.
authors.forename: Zhong-Qiong
authors.initials: ZQ
authors.lastname: Yin
authors.affiliation: Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern 0316 Oslo, Norway. b.s.paulsen@farmasi.uio.no.
authors.forename: Berit Smestad
authors.initials: BS
authors.lastname: Paulsen
mesh_terms: D002848:Chromatography, DEAE-Cellulose; D002849:Chromatography, Gas; D003167:Complement Activation; D003168:Complement Fixation Tests; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005685:Galactans / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D030004:Ligusticum / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D009005:Monosaccharides / Q000737:chemistry; D010368:Pectins / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D027343:Rhizome / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D004365:Drugs, Chinese Herbal; D005685:Galactans; D009005:Monosaccharides; C534193:chuanxiong rhizome preparation; C042491:rhamnogalacturonan I; D010368:Pectins; C005653:arabinogalactan
keywords: Ligusticum chuanxiong; complement system; pectic polysaccharide; polysaccharides; purification