title: Rationally Designed Peptide Probes for Extracellular Vesicles.
abstract: Extracellular vesicles (EVs) are submicroscopic lipid vesicles secreted from cells and play significant roles in cell-to-cell communication by transporting varieties of cell signaling molecules like proteins, DNA, mRNA, and microRNA. Recent studies showed that EVs are highly correlated with cancer progression and metastasis. However, there are some difficulties in probing each vesicle using popular analytical methods because of their small sizes and heterogeneous origins. These obstacles may be overcome by using a novel approach that senses highly curved membrane and negatively charged membrane lipids. In this chapter, we highlight the basic biological concepts of EVs, isolation, and quantification methods, and recent advent of peptide probes for EVs.
authors.affiliation: BioFrontiers Institute, University of Colorado Boulder, Boulder, CO, United States.
authors.forename: R
authors.initials: R
authors.lastname: Tamura
authors.affiliation: BioFrontiers Institute, University of Colorado Boulder, Boulder, CO, United States. Electronic address: Hubert.yin@colorado.edu.
authors.forename: H
authors.initials: H
authors.lastname: Yin
mesh_terms: D055354:Exosomes / Q000737:chemistry; D015335:Molecular Probes / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*
chemical_list: D015335:Molecular Probes; D010455:Peptides
keywords: Biomarkers; Cancer detection; Curvature/lipid-sensing peptides; Drug delivery; Extracellular vesicles; Membrane curvature