title: Molecular characterisation of tumour necrosis factor alpha and its potential connection with lipoprotein lipase and peroxisome proliferator-activated receptors in blunt snout bream (Megalobrama amblycephala).
abstract: Tumour necrosis factor alpha (TNF-α) is one kind of cytokines which is related to inflammation and lipid metabolism. TNF-α cDNA was cloned from the liver of blunt snout bream (Megalobrama amblycephala) through real-time polymerase chain reaction (PCR) and rapid amplification of cDNA ends (RACE) methods. The full-length cDNA of TNF-α covered 1467 bp, with an open reading frame (ORF) of 723 bp, which encodes 240 amino acids. It possessed the TNF family signature IIIPDDGIYFVYSQ. After the lipopolysaccharide (LPS) challenge test, a graded tissue-specific expression pattern of TNF-α was observed and there was high expression abundance in the kidney, brain and liver. After 8 weeks feeding trial, liver samples, two groups fed with 6% and 11% lipid levels, were collected. The results showed that, for fish fed with high-fat diet, the triglyceride of serum and lipid content of liver were elevated. Furthermore, TNF-α and peroxisome proliferator-activated receptors (PPARα, β) mRNA expression of fish fed 11% lipid diet were significantly up-regulated (p < 0.05). Lipoprotein lipase (LPL) and PPARγ mRNA expression of fish fed 11% lipid lever diet were significantly decreased compared to those of fish fed 6% (p < 0.05). The differences between the various expression of related genes in the high and low fat groups demonstrated that TNF-α played a key role in lipid metabolism, which may have an influence on fat metabolism through reducing fat synthesis and strengthening the β-oxidation of fatty acid. These discrepancies warrant further research.
authors.affiliation: Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing, 210095, Jiangsu Province, People's Republic of China.
authors.forename: Man
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Tongwei Company Limited, No. 588 Middle Section of Tianfu Avenue, Chengdu Hi-Tech Zone, Chengdu, 610041, People's Republic of China.
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Mi
authors.affiliation: Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing, 210095, Jiangsu Province, People's Republic of China.
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Liu
authors.affiliation: Tongwei Company Limited, No. 588 Middle Section of Tianfu Avenue, Chengdu Hi-Tech Zone, Chengdu, 610041, People's Republic of China.
authors.forename: Ye-Yang
authors.initials: YY
authors.lastname: Wu
authors.affiliation: Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing, 210095, Jiangsu Province, People's Republic of China.
authors.forename: Kai-Zhou
authors.initials: KZ
authors.lastname: Wang
authors.affiliation: Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing, 210095, Jiangsu Province, People's Republic of China. jianggz@njau.edu.cn.
authors.forename: Guang-Zhen
authors.initials: GZ
authors.lastname: Jiang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003530:Cyprinidae / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D059305:Diet, High-Fat; D050356:Lipid Metabolism; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D016366:Open Reading Frames; D047492:Peroxisome Proliferator-Activated Receptors / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D014280:Triglycerides / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D047492:Peroxisome Proliferator-Activated Receptors; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha; D008071:Lipoprotein Lipase
keywords: Gene expression; Lipoprotein lipase (LPL); Megalobrama amblycephala; Peroxisome proliferator-activated receptors (PPARs); Tumour necrosis factor alpha (TNF-α)