title: Effects of renal denervation on monocrotaline induced pulmonary remodeling.
abstract: Pulmonary artery hypertension (PAH) is a rapidly progressive disorder, which leads to right heart failure and even death. Overactivity of the renin-angiotensin-aldosterone system (RAAS) and sympathetic nervous system accounts for the development and progression of PAH. The role of renal denervation (RDN) in different periods of PAH has not been fully elucidated. A single intraperitoneal injection of monocrotaline (MCT, 60 mg/kg) was used to induce pulmonary remodeling in male Sprague Dawley rats (n = 40). After 24-hour of MCT administration, a subset of rats underwent RDN (RDN24h, n = 10); after 2-week of MCT injection, another ten rats received RDN treatment (RDN2w, n = 10) and the left 20 rats were divided to MCT group with sham RDN operation (MCT, n = 20). Eight rats in Control group received intraperitoneal injection of normal saline (60 mg/kg) once and sham RDN surgery. After 35 days, tissue and blood samples were collected. Histological analysis demonstrated that the collagen volume fraction of right ventricle, lung tissue and pulmonary vessel reduced significantly in RDN24h group but not in the RDN2w group, compared with MCT group. Moreover, the earlier RDN treatment significantly decreased SNS activity and blunted RAAS activation. Importantly, RDN treatment significantly improved the survival rate. In summary, earlier RDN treatment could attenuate cardio-pulmonary fibrosis and therefore might be a promising approach to prevent the development of PAH.
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Cardiology, Gansu Provincial Hospital, Lanzhou, China.
authors.forename: Jiyang
authors.initials: J
authors.lastname: Song
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Dasheng
authors.initials: D
authors.lastname: Lu
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Geng
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Qijun
authors.initials: Q
authors.lastname: Shan
mesh_terms: D056151:Airway Remodeling / Q000187:drug effects*; D000818:Animals; D001808:Blood Vessels / Q000187:drug effects / Q000473:pathology; D003714:Denervation; D004195:Disease Models, Animal; D005355:Fibrosis; D006352:Heart Ventricles / Q000473:pathology; D006976:Hypertension, Pulmonary / Q000175:diagnosis / Q000209:etiology / Q000473:pathology / Q000503:physiopathology; D053208:Kaplan-Meier Estimate; D007668:Kidney / Q000294:innervation*; D008168:Lung / Q000187:drug effects* / Q000473:pathology*; D008297:Male; D016686:Monocrotaline / Q000009:adverse effects*; D051381:Rats; D012084:Renin-Angiotensin System
chemical_list: D016686:Monocrotaline
keywords: monocrotaline; pulmonary remodeling; renal denervation