title: The crystal structure of the Hsp90 co-chaperone Cpr7 from Saccharomyces cerevisiae.
abstract: The versatility of Hsp90 can be attributed to the variety of co-chaperone proteins that modulate the role of Hsp90 in many cellular processes. As a co-chaperone of Hsp90, Cpr7 is essential for accelerating the cell growth in an Hsp90-containing trimeric complex. Here, we report the crystal structure of Cpr7 at a resolution of 1.8Å. It consists of an N-terminal PPI domain and a C-terminal TPR domain, and exhibits a U-shape conformation. Our studies revealed the aggregation state of Cpr7 in solution and the interaction properties between Cpr7 and the MEEVD sequence from the C-terminus of Hsp90. In addition, the structure and sequence analysis between Cpr7 and homologues revealed the structure basis both for the function differences between Cpr6 and Cpr7 and the functional complements between Cns1 and Cpr7. Our studies facilitate the understanding of Cpr7 and provide decent insights into the molecular mechanisms of the Hsp90 co-chaperone pathway.
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale, Innovation Centre for Cell Signalling Network, School of Life Science, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale, Innovation Centre for Cell Signalling Network, School of Life Science, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.
authors.forename: Qiangqiang
authors.initials: Q
authors.lastname: Ge
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale, Innovation Centre for Cell Signalling Network, School of Life Science, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.
authors.forename: Mingxing
authors.initials: M
authors.lastname: Wang
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale, Innovation Centre for Cell Signalling Network, School of Life Science, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Lv
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale, Innovation Centre for Cell Signalling Network, School of Life Science, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Ebrahimi
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale, Innovation Centre for Cell Signalling Network, School of Life Science, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.
authors.forename: Liwen
authors.initials: L
authors.lastname: Niu
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale, Innovation Centre for Cell Signalling Network, School of Life Science, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China. Electronic address: mkteng@ustc.edu.cn.
authors.forename: Maikun
authors.initials: M
authors.lastname: Teng
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale, Innovation Centre for Cell Signalling Network, School of Life Science, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China. Electronic address: sachem@ustc.edu.cn.
authors.forename: Xu
authors.initials: X
authors.lastname: Li
mesh_terms: D018360:Crystallography, X-Ray; D018841:HSP90 Heat-Shock Proteins / Q000737:chemistry / Q000378:metabolism*; D018832:Molecular Chaperones / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D000072417:Protein Domains; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D018841:HSP90 Heat-Shock Proteins; D018832:Molecular Chaperones; D029701:Saccharomyces cerevisiae Proteins
keywords: Co-chaperone; Cpr7; Crystal structure; Hsp90; TPR domain