title: Syringa oblata Lindl. Aqueous Extract Is a Potential Biofilm Inhibitor inS. suis.
abstract: Streptococcus suis(S. suis) is a zoonotic pathogen that causes severe disease symptoms in pigs and humans.Syringa oblata Lindl.distributed in the middle latitudes of Eurasia and North America were proved as the most development potential of Chinese Medicine. In this study, biofilm formation byS. suisdecreased after growth with 1/2 MIC, 1/4 MIC, or 1/8 MIC ofSyringa oblata Lindl.aqueous extract and rutin. Scanning electron microscopy analysis revealed the potential effect ofSyringa oblata Lindl.aqueous extract and rutin against biofilm formation byS. suis. Using iTRAQ technology, comparative proteomic analyses was performed at two conditions: 1/2 MIC ofSyringa oblata Lindl.aqueous extract treated and non-treated cells. The results revealed the existence of 28 proteins of varying amounts. We found that the majority of the proteins were related to cell growth and metabolism. We also found thatSyringa oblata Lindl.Aqueous extract affected the synthesis enzymes. In summary,Syringa oblata Lindl.aqueous extract might be used to inhibit the biofilm formation effectively byS. suis, and the active ingredients of theSyringa oblate Lindl.aqueous extract is rutin. The content of rutin is 9.9 ± 0.089 mg/g dry weight.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University Harbin, China.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Bai
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University Harbin, China.
authors.forename: Yanbei
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University Harbin, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University Harbin, China.
authors.forename: Lingfei
authors.initials: L
authors.lastname: Gao
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University Harbin, China.
authors.forename: Jianqing
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University Harbin, China.
authors.forename: Yongzhi
authors.initials: Y
authors.lastname: Ren
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University Harbin, China.
authors.forename: Wenya
authors.initials: W
authors.lastname: Ding
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University Harbin, China.
authors.forename: Ishfaq
authors.initials: I
authors.lastname: Muhammad
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University Harbin, China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Li
keywords: Streptococcus suis; Syringa oblata Lindl.; biofilm formation; proteomics