title: Spontaneous ultra-weak photon emission in correlation to inflammatory metabolism and oxidative stress in a mouse model of collagen-induced arthritis.
abstract: The increasing prevalence of rheumatoid arthritis has driven the development of new approaches and technologies for investigating the pathophysiology of this devastating, chronic disease. From the perspective of systems biology, combining comprehensive personal data such as metabolomics profiling with ultra-weak photon emission (UPE) data may provide key information regarding the complex pathophysiology underlying rheumatoid arthritis. In this article, we integrated UPE with metabolomics-based technologies in order to investigate collagen-induced arthritis, a mouse model of rheumatoid arthritis, at the systems level, and we investigated the biological underpinnings of the complex dataset. Using correlation networks, we found that elevated inflammatory and ROS-mediated plasma metabolites are strongly correlated with a systematic reduction in amine metabolites, which is linked to muscle wasting in rheumatoid arthritis. We also found that increased UPE intensity is strongly linked to metabolic processes (with correlation co-efficiency |r| value >0.7), which may be associated with lipid oxidation that related to inflammatory and/or ROS-mediated processes. Together, these results indicate that UPE is correlated with metabolomics and may serve as a valuable tool for diagnosing chronic disease by integrating inflammatory signals at the systems level. Our correlation network analysis provides important and valuable information regarding the disease process from a system-wide perspective.
authors.affiliation: Analytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands; Sino-Dutch Center for Preventive and Personalized Medicine, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
authors.forename: Min
authors.initials: M
authors.lastname: He
authors.affiliation: Analytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands; Sino-Dutch Center for Preventive and Personalized Medicine, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands; Meluna Research, Geldermalsen, The Netherlands; Changchun University of Chinese Medicine, No. 1035, Boshuo Rd, Jingyue Economic Development District, Changchun 130117, China. Electronic address: eduard.vanwijk@sinodutchcentre.nl.
authors.forename: Eduard
authors.initials: E
authors.lastname: van Wijk
authors.affiliation: Analytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands; Sino-Dutch Center for Preventive and Personalized Medicine, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands; TNO, P.O. Box 360, 3700 AJ Zeist, The Netherlands.
authors.forename: Herman
authors.initials: H
authors.lastname: van Wietmarschen
authors.affiliation: Analytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands; Sino-Dutch Center for Preventive and Personalized Medicine, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands; SU Biomedicine, Utrechtseweg 48, 3700 AJ Zeist, The Netherlands; Changchun University of Chinese Medicine, No. 1035, Boshuo Rd, Jingyue Economic Development District, Changchun 130117, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.affiliation: Analytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands; Sino-Dutch Center for Preventive and Personalized Medicine, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands; Changchun University of Chinese Medicine, No. 1035, Boshuo Rd, Jingyue Economic Development District, Changchun 130117, China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Sun
authors.affiliation: Analytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
authors.forename: Slavik
authors.initials: S
authors.lastname: Koval
authors.affiliation: Sino-Dutch Center for Preventive and Personalized Medicine, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands; Meluna Research, Geldermalsen, The Netherlands.
authors.forename: Roeland
authors.initials: R
authors.lastname: van Wijk
authors.affiliation: Analytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands; Sino-Dutch Center for Preventive and Personalized Medicine, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
authors.forename: Thomas
authors.initials: T
authors.lastname: Hankemeier
authors.affiliation: Analytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands; Sino-Dutch Center for Preventive and Personalized Medicine, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands; TNO, P.O. Box 360, 3700 AJ Zeist, The Netherlands.
authors.forename: Jan
authors.initials: J
authors.lastname: van der Greef
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000139:chemically induced* / Q000473:pathology; D001172:Arthritis, Rheumatoid / Q000503:physiopathology; D003094:Collagen; D007249:Inflammation / Q000378:metabolism*; D015227:Lipid Peroxidation; D055432:Metabolomics / Q000379:methods*; D051379:Mice; D018384:Oxidative Stress; D017785:Photons; D017382:Reactive Oxygen Species; D049490:Systems Biology / Q000379:methods
chemical_list: D017382:Reactive Oxygen Species; D003094:Collagen
keywords: Collagen-induced arthritis; Correlation networks; Metabolomics; Systems biology; Ultra-weak photon emission