title: Characterization of the pJB12 Plasmid from Pseudomonas aeruginosa Reveals Tn6352, a Novel Putative Transposon Associated with Mobilization of theblaVIM-2-Harboring In58 Integron.
abstract: TheblaVIM-2-carrying In58 integron has been linked to a chromosomal location in different bacterial species, includingPseudomonas aeruginosaThis work reports the first fully sequenced In58-harboring plasmid, which is significantly different from the two previously identifiedblaVIM-2-carrying plasmids inP. aeruginosablaVIM-2might have been acquired by transposition of Tn6352, a novel transposon composed of the In58 and ISPa17elements. The recognition of similar inverted repeat (IR) sites by ISPa17reveals a common mobilization process associated with acquisition of theblaVIM-2andblaVIM-1genes.
authors.affiliation: UCIBIO/REQUIMTE, Laboratório de Microbiologia, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal.
authors.forename: João
authors.initials: J
authors.lastname: Botelho
authors.affiliation: UCIBIO/REQUIMTE, Laboratório de Microbiologia, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal.
authors.forename: Filipa
authors.initials: F
authors.lastname: Grosso
authors.affiliation: UCIBIO/REQUIMTE, Laboratório de Microbiologia, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal lpeixe@ff.up.pt.
authors.forename: Luísa
authors.initials: L
authors.lastname: Peixe
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D032023:Integrons / Q000235:genetics; D055029:Inverted Repeat Sequences / Q000235:genetics; D058885:Multilocus Sequence Typing; D010957:Plasmids / Q000235:genetics; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; C425108:beta-lactamase bla(vim-2); D001618:beta-Lactamases; C063912:carbapenemase
keywords: Pseudomonas aeruginosa; carbapenemase; plasmid-mediated resistance; transposon