title: Exposure to placental ischemia impairs postpartum maternal renal and cardiac function in rats.
abstract: Women with a history of preeclampsia (PE) have an increased risk to develop cardiovascular and renal diseases later in life, but the mechanisms underlying this effect are unknown. In rats, we assessed whether placental ischemia results in long-term effects on the maternal cardiovascular and renal systems using the reduced uterine perfusion pressure (RUPP) model for PE. Sprague-Dawley rats received either a Sham or RUPP operation at gestationalday 14The rats were followed for 8 wk after delivery (Shamn= 12, RUPPn= 21) at which time mean arterial pressure (MAP; conscious), 24-h albuminuria, glomerular filtration rate (GFR; transcutaneous, FITC-sinistrin), and cardiac function (Vevo 770 system) were assessed. Subsequently, all rats were euthanized for mesenteric artery vasorelaxation and histology of heart and kidney. At 8 wk after delivery, there was no difference in MAP and albuminuria. However, RUPP rats showed a significantly reduced GFR [2.61 ± 0.53 vs. 3.37 ± 0.74 ml/min;P= 0.01]. Ultrasound showed comparable cardiac structure, but RUPP rats had a lower left ventricular ejection fraction (62 ± 7 vs. 69 ± 10%;P= 0.04). Heart and kidney histology was not different between Sham or RUPP rats. Furthermore, there were no differences in endothelial-dependent or -independent vasorelaxation. We show that exposure to placental ischemia in rats is accompanied by functional disturbances in maternal renal and cardiac function 8 wk after a preeclamptic pregnancy. However, these changes were not dependent on differences in blood pressure, small artery vasorelaxation, or cardiac and renal structure at this time point postpartum.
authors.affiliation: Department of Obstetrics, Wilhelmina Children's Hospital Birth Center, University Medical Center Utrecht, Utrecht, The Netherlands; n.d.paauw-2@umcutrecht.nl.
authors.forename: Nina D
authors.initials: ND
authors.lastname: Paauw
authors.affiliation: Department of Nephrology and Hypertension, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Jaap A
authors.initials: JA
authors.lastname: Joles
authors.affiliation: Department of Physiology, University of Mississippi Medical Center, Jackson, Mississippi; and.
authors.forename: Frank T
authors.initials: FT
authors.lastname: Spradley
authors.affiliation: Department of Physiology, University of Mississippi Medical Center, Jackson, Mississippi; and.
authors.forename: Bhavisha
authors.initials: B
authors.lastname: Bakrania
authors.affiliation: Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts.
authors.forename: Zsuzsanna K
authors.initials: ZK
authors.lastname: Zsengeller
authors.affiliation: Department of Obstetrics, Wilhelmina Children's Hospital Birth Center, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Arie
authors.initials: A
authors.lastname: Franx
authors.affiliation: Department of Nephrology and Hypertension, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Marianne C
authors.initials: MC
authors.lastname: Verhaar
authors.affiliation: Department of Physiology, University of Mississippi Medical Center, Jackson, Mississippi; and.
authors.forename: Joey P
authors.initials: JP
authors.lastname: Granger
authors.affiliation: Department of Obstetrics, Wilhelmina Children's Hospital Birth Center, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: A Titia
authors.initials: AT
authors.lastname: Lely
mesh_terms: D000818:Animals; D005260:Female; D005919:Glomerular Filtration Rate; D006331:Heart Diseases / Q000209:etiology / Q000503:physiopathology*; D006334:Heart Function Tests; D007511:Ischemia / Q000150:complications / Q000503:physiopathology; D007674:Kidney Diseases / Q000209:etiology / Q000503:physiopathology*; D018811:Maternal Exposure; D010920:Placenta / Q000098:blood supply / Q000503:physiopathology*; D011225:Pre-Eclampsia / Q000503:physiopathology*; D011247:Pregnancy; D011249:Pregnancy Complications, Cardiovascular / Q000503:physiopathology*; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017207:Rats, Sprague-Dawley
keywords: cardiac function; glomerular filtration rate; placental ischemia; postpartum; preeclampsia