title: A Label-free "Turn-on" Fluorescence Method for Detecting Mercury Ion in Aqueous Solution Based on N-Methyl Mesoporphyrin IX (NMM)/G-quadruplex DNA.
abstract: In the present work, a simple design of "turn-on" fluorescence method for mercury ion was developed and explored based on specific T-Hg-T mismatches as a recognizer and N-methyl mesoporphyrin IX (NMM)/G-quadruplex DNA system as a reporter. The titration experiment showed that the mercury-ion concentration and the fluorescence intensity signal change exhibited a consistent linear correlation within the 50 to 500 nM range with a detection limit down to 12.9 nM. In a selectivity experiment, our method showed obvious selectivity against other metal ions, being consistent with other reported detection methods of mercury ion based on the specific reactivity of T-Hg-T mismatches. Our method was then successfully employed to detect mercury ion in pond water with excellent reliability.
authors.affiliation: Jingchu Food Research and Development Center, College of Life Science, Yangtze University.
authors.forename: Xingping
authors.initials: X
authors.lastname: Zhang
authors.forename: Baomiao
authors.initials: B
authors.lastname: Ding
authors.forename: Huawei
authors.initials: H
authors.lastname: Wu
authors.forename: Jiujun
authors.initials: J
authors.lastname: Wang
authors.forename: Hualin
authors.initials: H
authors.lastname: Yang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D054856:G-Quadruplexes; D008628:Mercury / Q000032:analysis*; D008652:Mesoporphyrins / Q000737:chemistry*; D012996:Solutions; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry*
chemical_list: D008652:Mesoporphyrins; D012996:Solutions; D014867:Water; C031468:mesoporphyrin IX; D004247:DNA; D008628:Mercury