title: Escherichia coli sequence type 73 as a cause of community acquired urinary tract infection in men and women in Rio de Janeiro, Brazil.
abstract: Escherichia coli clones ST131, ST69, ST95, and ST73 are frequent causes of urinary tract infections (UTI) and bloodstream infections. Specific clones and virulence profiles of E. coli causing UTI in men has been rarely described. The aim of this study was to characterize patient and clonal characteristics of community-acquired UTI caused by E. coli in men (n=12) and women (n=127) in Rio de Janeiro, Brazil, complementing a previous work. We characterized isolates in phylogenetic groups, ERIC2-PCR and PFGE types, MLST, genome similarity and virulence gene-profiles. UTI from men were more frequently caused by phylogenetic group B2 isolates (83% versus 42%, respectively, P = 0.01), a group with significantly higher virulence scores compared with women. ST73 was the predominant clone in men (50%) and the second most frequent in women (12%), with the highest virulence score (mean and median=9) among other clones. ST73 gnomes formed at least six clusters. E. coli from men carried significantly higher numbers of virulence genes, such as sfa/focDE (67% versus 27%), hlyA (58% versus 24%), cnf 1 (58% versus 16%), fyuA (100% versus 82%) and MalX (92% versus 44%), compared with isolates from women. These data suggest the predominance and spread of ST73 isolates likely relates to an abundance of virulence determinants.
authors.affiliation: Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Rio de Janeiro, 21941-902, Brazil. Electronic address: bmeurer@micro.ufrj.br.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: de Souza da-Silva
authors.affiliation: Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Rio de Janeiro, 21941-902, Brazil.
authors.forename: Viviane Santos
authors.initials: VS
authors.lastname: de Sousa
authors.affiliation: Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Rio de Janeiro, 21941-902, Brazil.
authors.forename: Natacha
authors.initials: N
authors.lastname: Martins
authors.affiliation: Instituto Biomédico, Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Rubens Clayton
authors.initials: RC
authors.lastname: da Silva Dias
authors.affiliation: Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Rio de Janeiro, 21941-902, Brazil.
authors.forename: Raquel Regina
authors.initials: RR
authors.lastname: Bonelli
authors.affiliation: School of Public Health, University of California, Berkeley, CA.
authors.forename: Lee W
authors.initials: LW
authors.lastname: Riley
authors.affiliation: Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Rio de Janeiro, 21941-902, Brazil. Electronic address: bmeurer@micro.ufrj.br.
authors.forename: Beatriz Meurer
authors.initials: BM
authors.lastname: Moreira
mesh_terms: D001938:Brazil / Q000453:epidemiology; D017714:Community-Acquired Infections / Q000453:epidemiology* / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology* / Q000382:microbiology*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D058885:Multilocus Sequence Typing; D016133:Polymerase Chain Reaction; D014552:Urinary Tract Infections / Q000453:epidemiology* / Q000382:microbiology*; D037521:Virulence Factors / Q000235:genetics
chemical_list: D037521:Virulence Factors
keywords: ST73; Urinary tract infection; Uropathogenic Escherichia coli; Virulence factors