title: Measurement of Macrophage-Specific In Vivo Reverse Cholesterol Transport in Mice.
abstract: Reverse cholesterol transport (RCT) is one of the main processes that is thought to protect against cardiovascular disease. RCT constitutes the removal of cholesterol from peripheral sites, its transport through the plasma compartment for delivery to the liver for excretion. Here, we describe an in vivo RCT method that incorporates these steps, measuring movement of cholesterol from macrophages to the plasma, the liver, and finally to the feces in mice.
authors.affiliation: ANZAC Research Institute, Concord Repatriation General Hospital, Concord, NSW, 2139, Australia. maaike.kockx@sydney.edu.au.
authors.forename: Maaike
authors.initials: M
authors.lastname: Kockx
authors.affiliation: ANZAC Research Institute, Concord Repatriation General Hospital, Concord, NSW, 2139, Australia.
authors.forename: Wendy
authors.initials: W
authors.lastname: Jessup
authors.affiliation: ANZAC Research Institute, Concord Repatriation General Hospital, Concord, NSW, 2139, Australia.
authors.forename: Leonard
authors.initials: L
authors.lastname: Kritharides
mesh_terms: D000818:Animals; D001693:Biological Transport, Active / Q000502:physiology; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism*; D005243:Feces; D008099:Liver / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D051379:Mice
chemical_list: D002784:Cholesterol
keywords: In vivo RCT; Macrophage-specific RCT; Reverse cholesterol transport