title: Reagent-free simultaneous determination of glucose and cholesterol in whole blood by FTIR-ATR.
abstract: Reagent-free determination of multiple analytes is an active and promising field of research in clinical analysis. In this work, the determination of glucose and cholesterol in whole blood using Fourier transform infrared (FTIR) spectroscopy equipped with an attenuated total reflectance (ATR) accessory was performed. A comprehensive sample selection rule in multi space based on SPXY was proposed, termed C-SPXY. The core idea is to make full use of different derivative spectra space to construct the calibration set which preserves the more effective information. On this basis, a partial least squares (PLS) regression fusion modeling method was also presented aiming at improving prediction accuracy of glucose and cholesterol concentration in whole blood samples. Compared with other methods based on single spectra space, the proposed fusion model based on multi spectra space C-SPXY method provides smaller RMSEP values. Experimental results demonstrate that the proposed method and model provides superior predictive power and holds a good application prospect in the field of clinical analysis.
authors.affiliation: College of Information Science and Engineering, Northeastern University, Shenyang, Liaoning 110819, China. Electronic address: lizhigang@neuq.edu.cn.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Haigang hospital, Qinhuangdao, Hebei Province 066000, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Lv
authors.affiliation: Haigang hospital, Qinhuangdao, Hebei Province 066000, China.
authors.forename: Tianhe
authors.initials: T
authors.lastname: Li
authors.affiliation: College of Information Science and Engineering, Northeastern University, Shenyang, Liaoning 110819, China.
authors.forename: Guangyuan
authors.initials: G
authors.lastname: Si
authors.affiliation: College of Information Science and Engineering, Northeastern University, Shenyang, Liaoning 110819, China.
authors.forename: Qiaoyun
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Information Science and Engineering, Northeastern University, Shenyang, Liaoning 110819, China.
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Lv
authors.affiliation: College of Information Science and Engineering, Northeastern University, Shenyang, Liaoning 110819, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Hu
mesh_terms: D001786:Blood Glucose / Q000032:analysis*; D002138:Calibration; D002784:Cholesterol / Q000097:blood*; D006801:Humans; D007202:Indicators and Reagents; D012015:Reference Standards; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001786:Blood Glucose; D007202:Indicators and Reagents; D002784:Cholesterol
keywords: Comprehensive sample selection; Derivative spectra; Fourier transform infrared (FTIR) spectroscopy; Fusion modeling; Whole blood