title: A novel photopolymerizable derivative of hyaluronan for designed hydrogel formation.
abstract: A new photopolymerizable derivative of hyaluronan (methacrylhydrazide-HA, MAHA) was prepared by carbodiimide chemistry. The reaction conditions were optimized for molecular weight (Mw), reaction time and amount of reagents with a degree of methacrylation (DM) ranging from 2% to 58%. Methacrylhydrazide-HA was hydrolytically stable (PBS, 7days, 37°C) in contrast to commonly used methacrylester analoque (23% hydrolyzed). MAHA readily photopolymerized into densely crosslinked hydrogels under physiological conditions. The varied DM, Mw, irradiation time (texp) and macromer concentration in photocrosslinking afforded hydrogels with different physical (swelling ratio, degradation rate) and mechanical properties (stiffness, toughness). Three-dimensional fabrication and surface patterning of MAHA hydrogels were demonstrated by photolithography and light mediated micromolding. A live-dead assay with skin fibroblasts showed convenient biocompatibility of MAHA (16%, 116kDa) for potential scaffolding applications in tissue engineering and regenerative medicine.
authors.affiliation: Contipro a.s., Dolní Dobrouč 401, 56102, Czechia. Electronic address: bobula@contipro.com.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Bobula
authors.affiliation: Contipro a.s., Dolní Dobrouč 401, 56102, Czechia.
authors.forename: Radovan
authors.initials: R
authors.lastname: Buffa
authors.affiliation: Contipro a.s., Dolní Dobrouč 401, 56102, Czechia.
authors.forename: Martina
authors.initials: M
authors.lastname: Hermannová
authors.affiliation: Contipro a.s., Dolní Dobrouč 401, 56102, Czechia.
authors.forename: Lenka
authors.initials: L
authors.lastname: Kohutová
authors.affiliation: Contipro a.s., Dolní Dobrouč 401, 56102, Czechia.
authors.forename: Pavlína
authors.initials: P
authors.lastname: Procházková
authors.affiliation: Contipro a.s., Dolní Dobrouč 401, 56102, Czechia.
authors.forename: Hana
authors.initials: H
authors.lastname: Vágnerová
authors.affiliation: Contipro a.s., Dolní Dobrouč 401, 56102, Czechia.
authors.forename: Martin
authors.initials: M
authors.lastname: Čepa
authors.affiliation: Contipro a.s., Dolní Dobrouč 401, 56102, Czechia.
authors.forename: Lucie
authors.initials: L
authors.lastname: Wolfová
authors.affiliation: Contipro a.s., Dolní Dobrouč 401, 56102, Czechia.
authors.forename: Ondřej
authors.initials: O
authors.lastname: Židek
authors.affiliation: Contipro a.s., Dolní Dobrouč 401, 56102, Czechia.
authors.forename: Vladimír
authors.initials: V
authors.lastname: Velebný
mesh_terms: D002478:Cells, Cultured; D003432:Cross-Linking Reagents; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D020100:Hydrogels / Q000138:chemical synthesis* / Q000737:chemistry; D058105:Polymerization; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D003432:Cross-Linking Reagents; D020100:Hydrogels; D006820:Hyaluronic Acid
keywords: Crosslinking; Hyaluronan; Hydrogel; Photolithography; Polymerization