title: The Basis for Acyl Specificity in the Tafazzin Reaction.
abstract: Tafazzin is a mitochondrial enzyme that transfers fatty acids from phospholipids to lysophospholipids. Mutations in tafazzin cause abnormal molecular species of cardiolipin and the clinical phenotype of Barth syndrome. However, the mechanism by which tafazzin creates acyl specificity has been controversial. We have shown that the lipid phase state can produce acyl specificity in the tafazzin reaction, but others have reported that tafazzin itself carries enzymatic specificity. To resolve this issue, we replicated and expanded the controversial experiments,i.e.the transfer of different acyl groups from phosphatidylcholine to monolysocardiolipin by yeast tafazzin. Our data show that this reaction requires the presence of detergent and does not take place in liposomes but in mixed micelles. To separate thermodynamic (lipid-dependent) from kinetic (enzyme-dependent) parameters, we followed the accumulation of cardiolipin during the reaction from the initial state to the equilibrium state. The transacylation rates of different acyl groups varied over 2 orders of magnitude and correlated tightly with the concentration of cardiolipin in the equilibrium state (lipid-dependent parameter). In contrast, the rates by which different transacylations approached the equilibrium state were very similar (enzyme-dependent parameter). Furthermore, we found that tafazzin catalyzes the remodeling of cardiolipin by combinations of forward and reverse transacylations, essentially creating an equilibrium distribution of acyl groups. These data strongly support the idea that the acyl specificity of the tafazzin reaction results from the physical properties of lipids.
authors.affiliation: From the Departments of Anesthesiology and Cell Biology, New York University School of Medicine, New York, New York 10016 michael.schlame@med.nyu.edu.
authors.forename: Michael
authors.initials: M
authors.lastname: Schlame
authors.affiliation: From the Departments of Anesthesiology and Cell Biology, New York University School of Medicine, New York, New York 10016.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.affiliation: From the Departments of Anesthesiology and Cell Biology, New York University School of Medicine, New York, New York 10016.
authors.forename: Mindong
authors.initials: M
authors.lastname: Ren
mesh_terms: D000215:Acylation; D000217:Acyltransferases / Q000378:metabolism*; D002308:Cardiolipins / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D008823:Micelles; D010713:Phosphatidylcholines / Q000378:metabolism*; D013379:Substrate Specificity; D015003:Yeasts
chemical_list: D002308:Cardiolipins; D005656:Fungal Proteins; D008823:Micelles; D010713:Phosphatidylcholines; D000217:Acyltransferases
keywords: acyltransferase; cardiolipin; enzyme kinetics; lipid; mitochondria