title: Study on γH2AX Expression of Lymphocytes as a Biomarker In Radiation Biodosimetry.
abstract: Flow cytometry analysis was used to detect the changes of γH2AX protein expression in human peripheral blood lymphocytes. In the dose-effect study, the expression of γH2AX was detected 1 h after irradiation with 60Co γ-rays at doses of 0, 0.5, 1, 2, 4, and 6 Gy. Blood was cultivated for 0, 1, 2, 4, 6, 12, and 24 h after 4 Gy60Co γ-rays irradiation for the time-effect study. At the same time, the blood was divided into four treatment groups (ultraviolet [UV] irradiation,60Co γ-rays irradiation, UV plus60Co γ-rays irradiation, and control group) to detect the changes of protein expression of γH2AX. The results showed that the γH2AX protein expression was in dose-effect and time-effect relationship with60Co γ-rays. The peak expression of γH2AX was at 1 h after60Co γ-ray irradiation and began to decrease quickly. Compared to irradiation with60Co γ-rays alone, the expression of γH2AX was not significantly changed after irradiation with60Co γ-rays plus UV. Dose rate did not significantly change the expression of γH2AX. The expression of γH2AX induced by60Co γ-rays was basically consistent with the micein vivoandin vitro. The results revealed that the detection of γH2AX protein expression changes in peripheral blood lymphocyte by flow cytometry analysis is reasonable and may be useful for biodosimetry.
authors.affiliation: Chinese Center for Disease Control and Prevention Key Laboratory of Radiological Protection and Nuclear Emergency, Beijing 100088, PR China; National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, PR China; Chinese Center for Medical Response to Radiation Emergency, Ministry of Health, Beijing 100088, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Chinese Center for Disease Control and Prevention Key Laboratory of Radiological Protection and Nuclear Emergency, Beijing 100088, PR China; National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, PR China; Chinese Center for Medical Response to Radiation Emergency, Ministry of Health, Beijing 100088, PR China.
authors.forename: Gang
authors.initials: G
authors.lastname: Gao
authors.affiliation: Chinese Center for Disease Control and Prevention Key Laboratory of Radiological Protection and Nuclear Emergency, Beijing 100088, PR China; National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, PR China; Chinese Center for Medical Response to Radiation Emergency, Ministry of Health, Beijing 100088, PR China.
authors.forename: Jian Lei
authors.initials: JL
authors.lastname: Ruan
authors.affiliation: Chinese Center for Disease Control and Prevention Key Laboratory of Radiological Protection and Nuclear Emergency, Beijing 100088, PR China; National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, PR China; Chinese Center for Medical Response to Radiation Emergency, Ministry of Health, Beijing 100088, PR China.
authors.forename: Jian Xiang
authors.initials: JX
authors.lastname: Liu
keywords: 60Co γ-ray; DNA damage; biodosimetry; irradiation; γH2AX