title: Enhancing ammonium oxidizing bacteria activity was key to single-stage partial nitrification-anammox system treating low-strength sewage under intermittent aeration condition.
abstract: Intermittent aeration and bio-augmentation were integrated to enhance single-stage partial nitrification-anammox (SPN/A) stability over 235-day operational period treating low-strength sewage. The effect of bio-augmentation sludge (with different abundances of ammonium oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB)) was determined. Partial nitrification sludge based bio-augmentation increased the total nitrogen (TN) removal efficiency from 29.1% to 70%, followed by the nitrification sludge (from 38.1% to 65.4%), then the denitrifying phosphorus sludge (from 42.1% to 54.4%). The evolution of bacteria activity and communities showed that anammox activity increased with the enhancement of AOB activity, and higher AOB abundance led to higher anammox bacterial abundance despite high NOB abundance. The enhancement of AOB activity produced more nitrite, anammox bacteria gained more nitrite than NOB since intermittent aeration selectively inhibited NOB, thus the reactor stability enhanced substantially. This study highlights the significance of enhancing AOB activity to ensure long-term operational stability of SPN/A processes.
authors.affiliation: National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Miao
authors.affiliation: National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Baikun
authors.initials: B
authors.lastname: Li
authors.affiliation: National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Simeng
authors.initials: S
authors.lastname: Wang
authors.affiliation: National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China. Electronic address: pyz@bjut.edu.cn.
authors.forename: Yongzhen
authors.initials: Y
authors.lastname: Peng
mesh_terms: D064751:Ammonium Compounds / Q000378:metabolism*; D000693:Anaerobiosis; D001419:Bacteria / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology; D009566:Nitrates / Q000032:analysis; D058465:Nitrification; D009584:Nitrogen / Q000302:isolation & purification; D010084:Oxidation-Reduction; D012722:Sewage / Q000382:microbiology*; D018508:Water Purification / Q000379:methods*
chemical_list: D064751:Ammonium Compounds; D009566:Nitrates; D012722:Sewage; D009584:Nitrogen
keywords: Bio-augmentation; Intermittent aeration; Low-strength sewage; Single-stage partial nitrification-anammox; Stabilization enhancement