title: The widespread occurrence of tRNA-derived fragments inSaccharomyces cerevisiae.
abstract: Short RNAs derived from the cleavage of tRNA molecules are observed in most organisms. Their occurrence seems to be induced by stress conditions, but still little is known about their biogenesis and functions. We find that the recovery of tRNA fragments depends on the RNA isolation method. Using an optimized RNA extraction protocol and northern blot hybridization technique, we show that the tRNA-derived fragments in yeast are widespread in 12 different growth conditions. We did not observe significant stress-dependent changes in the amounts of tRNA fragments pool. Instead, we show the differential processing of almost all individual tRNAs. We also provide evidence that 3'-part-derived tRNA fragments are as abundant as the 5'- one inSaccharomyces cerevisiae. The resulting set ofS. cerevisiaetRNA fragments provides a robust basis for further experimental studies on biological functions of tRFs.
authors.affiliation: Institute of Bioorganic Chemistry Polish Academy of Sciences Poznan Poland.
authors.forename: Kamilla
authors.initials: K
authors.lastname: Bąkowska-Żywicka
authors.affiliation: Institute of Bioorganic Chemistry Polish Academy of Sciences Poznan Poland.
authors.forename: Anna M
authors.initials: AM
authors.lastname: Mleczko
authors.affiliation: Institute of Bioorganic Chemistry Polish Academy of Sciences Poznan Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Kasprzyk
authors.affiliation: Institute of Bioorganic Chemistry Polish Academy of Sciences Poznan Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Machtel
authors.affiliation: Department of Computational Biology Institute of Molecular Biology and Biotechnology Faculty of Biology A. Mickiewicz University in Poznan Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Żywicki
authors.affiliation: Institute of Bioorganic Chemistry Polish Academy of Sciences Poznan Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Twardowski
keywords: Saccharomyces cerevisiae; northern blot hybridization; small RNAs; tRNA; tRNA‐derived fragments