title: TPS46, a Rice Terpene Synthase Conferring Natural Resistance to Bird Cherry-Oat Aphid,Rhopalosiphum padi(Linnaeus).
abstract: Plant terpene synthases (TPSs) are key enzymes responsible for terpene biosynthesis, and can play important roles in defense against herbivore attack. In rice, the protein sequence of TPS46 was most closely related to maize TPS10. However, unlike maizetps10,tps46was also constitutively expressed in rice even in the absence of herbivore attack. Potential roles or constitutive emissions of specific volatiles may due to the constitutive expressions oftps46 in rice. Therefore, in the present study, RNA interference (Ri) and overexpression (Oe) rice lines were generated to investigate the potential function of TPS46 inOryza sativasp.japonica. Interestingly, the rice plants become more susceptible toRhopalosiphum padiwhen expression oftps46 was silenced compared with Wt in greenhouse conditions. Artificial infestation bioassays further confirmed that Ri rice lines were susceptible toR. padi, whereas Oe rice lines were repellent toR. padi. Based on GC-MS and ToF-MS analysis, a total of eight volatile products catalyzed by TPS46 in rice were identified. Among them, only limonene and Eβf could be detected in all the Ri, Oe, and Wt lines, whereas other six volatiles were only found in the blend of volatiles from Oe lines. Moreover, the amount of constitutive limonene and Eβf in the Ri lines was significantly lower than in Wt lines, while the amounts of these two volatiles in the Oe line were obviously higher than in control rice. Our data suggested that the constitutive emissions of Eβf and limonene regulated by the constitutive expression oftps46 may play a crucial role in rice defense againstR. padi.Consequently,tps46 could be a potential target gene to be employed for improving the resistance of plants to aphids.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesBeijing, China; Institute of Plant Protection, Jiangsu Academy of Agricultural SciencesNanjing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Sun
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Xinzheng
authors.initials: X
authors.lastname: Huang
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Yuese
authors.initials: Y
authors.lastname: Ning
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesBeijing, China; College of Plant Protection, Shandong Agricultural UniversityTai'an, China.
authors.forename: Weixia
authors.initials: W
authors.lastname: Jing
authors.affiliation: Department of Biological Chemistry and Crop Protection, Rothamsted Research Harpenden, UK.
authors.forename: Toby J A
authors.initials: TJ
authors.lastname: Bruce
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Fangjun
authors.initials: F
authors.lastname: Qi
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Qixia
authors.initials: Q
authors.lastname: Xu
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Kongming
authors.initials: K
authors.lastname: Wu
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Yuyuan
authors.initials: Y
authors.lastname: Guo
keywords: (E)-β-farnesene; Rhopalosiphum padi; insect–plant interactions; terpene syntheses; tps46