title: Optimization of simultaneous saccharification and fermentation conditions with amphipathic lignin derivatives for concentrated bioethanol production.
abstract: Amphipathic lignin derivatives (A-LDs) prepared from the black liquor of soda pulping of Japanese cedar are strong accelerators for bioethanol production under a fed-batch simultaneous enzymatic saccharification and fermentation (SSF) process. To improve the bioethanol production concentration, conditions such as reaction temperature, stirring program, and A-LDs loadings were optimized in both small scale and large scale fed-batch SSF. The fed-batch SSF in the presence of 3.0g/L A-LDs at 38°C gave the maximum ethanol production and a high enzyme recovery rate. Furthermore, a jar-fermenter equipped with a powerful mechanical stirrer was designed for 1.5L-scale fed-batch SSF to achieve rigorous mixing during high substrate loading. Finally, the 1.5L fed-batch SSF with a substrate loading of 30% (w/v) produced a high ethanol concentration of 87.9g/L in the presence of A-LDs under optimized conditions.
authors.affiliation: Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Ningning
authors.initials: N
authors.lastname: Cheng
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Koda
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tamai
authors.affiliation: Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Yamamoto
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan; Global Institute for Collaborative Research and Education, Hokkaido University, Sapporo 060-8589, Japan. Electronic address: takasuka@cen.agr.hokudai.ac.jp.
authors.forename: Taichi E
authors.initials: TE
authors.lastname: Takasuka
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan. Electronic address: uraki@for.agr.hokudai.ac.jp.
authors.forename: Yasumitsu
authors.initials: Y
authors.lastname: Uraki
mesh_terms: D061249:Batch Cell Culture Techniques; D056804:Biofuels / Q000032:analysis*; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism; D002480:Cellulase / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D008031:Lignin / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013696:Temperature
chemical_list: D056804:Biofuels; D000431:Ethanol; D008031:Lignin; D002480:Cellulase
keywords: Amphipathic lignin derivative; Bioethanol; Enzymatic saccharification; Fed-batch simultaneous saccharification and fermentation (SSF); Powerful mechanical stirrer