title: Prognostic Value of microRNA Signature in Patients with Gastric Cancers.
abstract: The occurrence of lymph node metastases (LNM) after endoscopic submucosal dissection (ESD) in patients with gastric cancer (GC) leads to poor prognosis. However, few biomarkers are available to predict LNM in GC patients. Thus, we measured expression of 6 cancer-related miRNAs using real-time RT-PCR in 102 GC samples that were randomized into a training set and a testing set (each, 51 cases). Using logistic regression, we identified 4-miRNA (miR-27b, miR-128, miR-100 and miR-214) signatures for predicting LNM in GC patients. Patients with high-risk scores for the 4-miRNA signature tended to have higher LNM than those with low-risk scores. Meanwhile, the ROC curve of the 4-miRNA signature was better for predicting LNM in GC patients. In addition, Cox regression analysis indicated that a 2-miRNA signature (miR-27b and miR-214) or a miR-214/N stage signature was predictive of survival for GC patients. This work describes a previously unrecognized 4-miRNA signature involved in LNM and a 2-miRNA signature or miR-214/N stage signature related to GC patients' survival.
authors.affiliation: Department of Pathology, Qilu Hospital, Shandong University, Jinan, P.R. China.|Department of Pathology, School of Medicine, Shandong University, Jinan, P.R. China.
authors.forename: Hai-Ting
authors.initials: HT
authors.lastname: Liu
authors.affiliation: Department of Pathology, Qilu Hospital, Shandong University, Jinan, P.R. China.|Department of Pathology, School of Medicine, Shandong University, Jinan, P.R. China.
authors.forename: Ya-Wen
authors.initials: YW
authors.lastname: Wang
authors.affiliation: Department of Pathology, Qilu Hospital, Shandong University, Jinan, P.R. China.|Department of Pathology, School of Medicine, Shandong University, Jinan, P.R. China.
authors.forename: Ai-Yan
authors.initials: AY
authors.lastname: Xing
authors.affiliation: Department of Pathology, Qilu Hospital, Shandong University, Jinan, P.R. China.|Department of Pathology, School of Medicine, Shandong University, Jinan, P.R. China.
authors.forename: Duan-Bo
authors.initials: DB
authors.lastname: Shi
authors.affiliation: Department of Pathology, Qilu Hospital, Shandong University, Jinan, P.R. China.
authors.forename: Hui-
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Qilu Hospital, Shandong University, Jinan, P.R. China.|Department of Pathology, School of Medicine, Shandong University, Jinan, P.R. China.
authors.forename: Xiang-Yu
authors.initials: XY
authors.lastname: Guo
authors.affiliation: Department of Pathology, School of Medicine, Shandong University, Jinan, P.R. China.|Department of Pathology, Qingdao Central Hospital, Qingdao, P.R. China.
authors.forename: Jing-
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Pathology, Qilu Hospital, Shandong University, Jinan, P.R. China.|Department of Pathology, School of Medicine, Shandong University, Jinan, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
mesh_terms: D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016015:Logistic Models; D008207:Lymphatic Metastasis / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D011379:Prognosis; D013274:Stomach Neoplasms / Q000235:genetics*; D016019:Survival Analysis
chemical_list: C544266:MIRN100 microRNA, human; C545189:MIRN128 microRNA, human; C547397:MIRN214 microRNA, human; C531388:MIRN27 microRNA, human; D035683:MicroRNAs