title: B cell activating factor (BAFF) selects IL-10-B cells over IL-10+B cells during inflammatory responses.
abstract: B cell activating factor (BAFF) regulates B cell maturation, survival, function, and plays a critical pathogenic role in autoimmune diseases. It remains unclear how BAFF affects IL-10-B cells versus regulatory B cells (Bregs) in inflammatory responses. In this study, we found that IL-10-expressing Bregs decreased in lupus-prone MRL/lpr mice and experimental allergic encephalomyelitis (EAE) mice. On blockade of the effects of BAFF with TACI-IgG, IL-10+Bregs were upregulated in MRL/lpr and EAE mice. In addition, BAFF expanded IL-10+B cells over IL-10-B cells under noninflammatory conditions in vitro, whereas it expanded IL-10-B cells over IL-10+B cells during inflammatory responses, such as stimulation with autoantigen and LPS. Finally, the selection of IL-10-B cells over IL-10+B cells by BAFF was dependent on BAFF receptors (BAFFR, TACI, and BCMA) that were upregulated by inflammatory responses. This study suggests that BAFF selects IL-10-B cells over IL-10+regulatory B cells via BAFF receptors in inflammatory responses.
authors.affiliation: Department of Rheumatology, First hospital of Jilin University, Changchun 130021, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850, China; College of Pharmacy, Henan University, Kaifeng 475004, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Jilin University, Changchun 130021, China.
authors.forename: Qilin
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850, China; Department of Biomedicine, Institute of Frontier Medical Sciences, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Zhiding
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850, China; Department of Nephrology, The 307th Hospital of Chinese People's Liberation Army, Beijing 100850, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Liu
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850, China; Laboratory of Cellular and Molecular Immunology, Henan University, Kaifeng, Henan 475001, China.
authors.forename: Gaizhi
authors.initials: G
authors.lastname: Zhu
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Yu
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850, China.
authors.forename: Gencheng
authors.initials: G
authors.lastname: Han
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850, China.
authors.forename: Guojiang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Hou
authors.affiliation: College of Pharmacy, Henan University, Kaifeng 475004, China.
authors.forename: Tianxiao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Laboratory of Cellular and Molecular Immunology, Henan University, Kaifeng, Henan 475001, China.
authors.forename: Yuanfang
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850, China.
authors.forename: Beifen
authors.initials: B
authors.lastname: Shen
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850, China. Electronic address: yinher2001@yahoo.com.
authors.forename: He
authors.initials: H
authors.lastname: Xiao
authors.affiliation: Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850, China. Electronic address: wang_renxi@yahoo.com.
authors.forename: Renxi
authors.initials: R
authors.lastname: Wang
mesh_terms: D000818:Animals; D053264:B-Cell Activating Factor / Q000276:immunology*; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D007249:Inflammation / Q000276:immunology*; D016753:Interleukin-10 / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D019463:Mice, Inbred MRL lpr
chemical_list: D053264:B-Cell Activating Factor; C556062:IL10 protein, mouse; C505720:Tnfsf13b protein, mouse; D016753:Interleukin-10
keywords: BAFF; EAE; Inflammatory response; Lupus; Regulatory B cells