title: Receptor-based screening assays for the detection of antibiotics residues - A review.
abstract: Consumer and regulatory agencies have a high concern to antibiotic residues in food producing animals, so appropriate screening assays of fast, sensitive, low cost, and easy sample preparation for the identification of these residues are essential for the food-safety insurance. Great efforts in the development of a high-throughput antibiotic screening assay have been made in recent years. Concerning the screening of antibiotic residue, this review elaborate an overview on the availability, advancement and applicability of antibiotic receptor based screening assays for the safety assessment of antibiotics usage (i.e. radio receptor assay, enzyme labeling assays, colloidal gold receptor assay, enzyme colorimetry assay and biosensor assay). This manuscript also tries to shed a light on the selection, preparation and future perspective of receptor protein for antibiotic residue detection. These assays have been introduced for the screening of numerous food samples. Receptor based screening technology for antibiotic detection has high accuracy. It has been concluded that at the same time, it can detect a class of drugs for certain receptor, and realize the multi-residue detection. These assays offer fast, easy and precise detection of antibiotics.
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Saeed
authors.initials: S
authors.lastname: Ahmed
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Jianan
authors.initials: J
authors.lastname: Ning
authors.affiliation: MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Guyue
authors.initials: G
authors.lastname: Cheng
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan 430070, China; The University of Agriculture Peshawar, Pakistan.
authors.forename: Ijaz
authors.initials: I
authors.lastname: Ahmad
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Mingyue
authors.affiliation: MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qu
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Mujahid
authors.initials: M
authors.lastname: Iqbal
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: M A B
authors.initials: MAB
authors.lastname: Shabbir
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan 430070, China; MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: yuan5802@mail.hzau.edu.cn.
authors.forename: Zonghui
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000378:metabolism; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004350:Drug Residues / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D011956:Receptors, Cell Surface
keywords: Antibiotics; Labeling technique; Receptor; Residue; Screening assays