title: Effects of Combined Simultaneous and Sequential Endostar and Cisplatin Treatment in a Mice Model of Gastric Cancer Peritoneal Metastases.
abstract: Objective. Aimed to study the effects of endostar and cisplatin using an in vivo imaging system (IVIS) in a model of peritoneal metastasis of gastric cancer.Methods. NUGC-4 gastric cancer cells transfected with luciferase gene (NUGC-4-Luc) were injected i.p. into nude mice. One week later, mice were randomly injected i.p.: group 1, cisplatin (d1-3) + endostar (d4-7); group 2, endostar (d1-4) + cisplatin (d5-7); group 3, endostar + cisplatin d1, 4, and 7; group 4, saline for two weeks. One week after the final administration, mice were sacrificed. Bioluminescent data, microvessel density (MVD), and lymphatic vessel density (LVD) were analyzed.Results. Among the four groups, there were no significant differences in the weights and in the number of cancer cell photons on days 1 and 8 (P> 0.05). On day 15, the numbers in groups 3 and 1 were less than that in group 2 (P< 0.05). On day 21, group 3 was significantly less than group 2 (P< 0.05). MVD of group 4 was less than that of groups 1 and 2 (P< 0.01). There was no significant difference between groups 2 and 3 (P> 0.05) or in LVD number among the four groups (P> 0.05).Conclusions. IVIS® was more useful than weight, volume of ascites, and number of peritoneal nodules. The simultaneous group was superior to sequential groups in killing cancer cells and inhibiting vascular endothelium. Cisplatin-endostar was superior to endostar-cisplatin in killing cancer cells, while the latter in inhibiting peritoneal vascular endothelium.
authors.affiliation: Department of Medical Oncology, The Affiliated Hospital of Hebei University, Baoding 071000, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Jia
authors.affiliation: Department of Animal Center, The Fourth Hospital, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Shuguang
authors.initials: S
authors.lastname: Ren
authors.affiliation: Department of Epidemiology and Health Statistics, School of Public Health, Hebei Medical University, Shijiazhuang 050011, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: Hebei Province China-Japan Friendship Center for Cancer Detection, Shijiazhuang 050017, China.
authors.forename: Jianing
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Medical Oncology, The Fourth Hospital, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Xinliang
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Hebei Province China-Japan Friendship Center for Cancer Detection, Shijiazhuang 050017, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Animal Center, The Fourth Hospital, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Palliative Care Center, Beijing Cancer Hospital, Beijing 100000, China.
authors.forename: Wei
authors.identifier: 0000-0001-7361-1118
authors.initials: W
authors.lastname: Liu