title: Fluorescein-Based Chromogenic and Ratiometric Fluorescence Probe for Highly Selective Detection of Cysteine and Its Application in Bioimaging.
abstract: A dual mode fluorescent probe, which is based on an integration of fluorescein and coumarin fluorophores, was developed for the discrimination of Cys from Hcy and GSH. This probe (2) shows the advantage of quick reaction (5 min) with Cys, resulting in a strong fluorescence turn-on response when excited at 450 nm. Notably, it also demonstrates the ratiometric fluorescence property while excited by a shorter wavelength (332 nm). All of results suggest probe 2 has a high selectivity toward Cys even in the presence of other amino acids, cations and anions. The detection limit of Cys was calculated as 0.084 μM, which was much lower than the intracellular concentration.1H NMR, MS and DFT calculation were used to reveal the detection mechanism further. Finally, this low cytotoxic probe was successfully applied in bioimaging within HepG2 cells.
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University , Lanzhou 730000, People's Republic of China.|Key Laboratory of Salt Lakes Resources and Chemistry, Qinghai Institute of Salt Lakes, Chinese Academy of Sciences , Xining 810008, People's Republic of China.
authors.forename: Zhen-Hai
authors.initials: ZH
authors.lastname: Fu
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University , Lanzhou 730000, People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Han
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University , Lanzhou 730000, People's Republic of China.
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Shao
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University , Lanzhou 730000, People's Republic of China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Fang
authors.affiliation: Key Laboratory of Salt Lakes Resources and Chemistry, Qinghai Institute of Salt Lakes, Chinese Academy of Sciences , Xining 810008, People's Republic of China.
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University , Lanzhou 730000, People's Republic of China.
authors.forename: Ya-Wen
authors.initials: YW
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University , Lanzhou 730000, People's Republic of China.
authors.forename: Yu
authors.identifier: 0000-0002-3862-632X
authors.initials: Y
authors.lastname: Peng
mesh_terms: D066241:Carbon-13 Magnetic Resonance Spectroscopy; D003374:Coumarins / Q000737:chemistry; D003545:Cysteine / Q000032:analysis*; D019793:Fluorescein / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D057230:Limit of Detection; D057054:Molecular Imaging / Q000379:methods*; D066244:Proton Magnetic Resonance Spectroscopy; D013050:Spectrometry, Fluorescence; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet
chemical_list: D003374:Coumarins; D005456:Fluorescent Dyes; C030123:coumarin; D003545:Cysteine; D019793:Fluorescein