title: Enzyme-Initiated Free-Radical Polymerization of Molecularly Imprinted Polymer Nanogels on a Solid Phase with an Immobilized Radical Source.
abstract: An enzyme-mediated synthetic approach is described for the preparation of molecularly imprinted polymer nanoparticles (MIP-NPs) in aqueous media. Horseradish peroxidase (HRP) was used to initiate the polymerization of methacrylate or vinyl monomers and cross-linkers by catalyzing the generation of free radicals. To prevent entrapment of the enzyme in the cross-linked polymer, and to enable it to be reused, HRP was immobilized on a solid support. MIPs based on 4-vinylpyridine and 1,4-bis(acryloyl)piperazine for the recognition of 2,4-dichlorophenoxyacetic acid (2,4-D) and salicylic acid were synthesized in an aqueous medium. MIPs for the protein trypsin were also synthesized. MIP nanoparticles with sizes between 50 and 300 nm were obtained with good binding properties, a good imprinting effect, and high selectivity for the target molecule. The reusability of immobilized HRP for MIP synthesis was shown for several batches.
authors.affiliation: Sorbonne Universités-Université de Technologie de Compiègne, CNRS Enzyme and Cell Engineering Laboratory, CS 60319, 60203, Compiègne cedex, France.|Université Libanaise, Laboratoire de Valorisation des Ressources Naturelles et Produits de Santé, Cité Universitaire Rafic Hariri, Hadath, Lebanon.
authors.forename: Mira
authors.initials: M
authors.lastname: Daoud Attieh
authors.affiliation: Sorbonne Universités-Université de Technologie de Compiègne, CNRS Enzyme and Cell Engineering Laboratory, CS 60319, 60203, Compiègne cedex, France.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Université Libanaise, Laboratoire de Valorisation des Ressources Naturelles et Produits de Santé, Cité Universitaire Rafic Hariri, Hadath, Lebanon.
authors.forename: Assem
authors.initials: A
authors.lastname: Elkak
authors.affiliation: Sorbonne Universités-Université de Technologie de Compiègne, CNRS Enzyme and Cell Engineering Laboratory, CS 60319, 60203, Compiègne cedex, France.
authors.forename: Aude
authors.initials: A
authors.lastname: Falcimaigne-Cordin
authors.affiliation: Sorbonne Universités-Université de Technologie de Compiègne, CNRS Enzyme and Cell Engineering Laboratory, CS 60319, 60203, Compiègne cedex, France.
authors.forename: Karsten
authors.initials: K
authors.lastname: Haupt
keywords: enzyme catalysis; free-radical polymerization; molecularly imprinted polymers; nanoparticles; oxidoreductase