title: Topical Formulation Containing Beeswax-Based Nanoparticles Improved In Vivo Skin Barrier Function.
abstract: Lipid nanoparticles have shown many advantages for treatment/prevention of skin disorders with damaged skin barrier function. Beeswax is a favorable candidate for the development of nanosystems in the cosmetic and dermatological fields because of its advantages for the development of products for topical application. In the present study, beeswax-based nanoparticles (BNs) were prepared using the hot melt microemulsion technique and incorporated to a gel-cream formulation. The formulation was subsequently evaluated for its rheological stability and effect on stratum corneum water content (SCWC) and transepidermal water loss (TEWL) using in vivo biophysical techniques. BNs resulted in mean particle size of 95.72 ± 9.63 nm and zeta potential of -9.85 ± 0.57 mV. BN-loaded formulation showed shear thinning behavior, well adjusted by the Herschel-Bulkley model, and a small thixotropy index that were stable for 28 days at different temperatures. BN-loaded formulation was also able to simultaneously decrease the TEWL and increase the SCWC values 28 days after treatment. In conclusion, the novel beeswax-based nanoparticles showed potential for barrier recovery and open the perspective for its commercial use as a novel natural active as yet unexplored in the field of dermatology and cosmetics for treatment of skin diseases with damaged skin barrier function.
authors.affiliation: School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Av. do Café s/n, Campus USP, 14040-903, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Carla
authors.initials: C
authors.lastname: Souza
authors.affiliation: School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Av. do Café s/n, Campus USP, 14040-903, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Luis Alexandre Pedro
authors.initials: LAP
authors.lastname: de Freitas
authors.affiliation: School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Av. do Café s/n, Campus USP, 14040-903, Ribeirão Preto, São Paulo, Brazil. pmcampos@usp.br.
authors.forename: Patrícia Maria Berardo Gonçalves
authors.initials: PMBG
authors.lastname: Maia Campos
mesh_terms: D000287:Administration, Topical; D000328:Adult; D003358:Cosmetics; D004339:Drug Compounding; D005260:Female; D006801:Humans; D008055:Lipids; D053758:Nanoparticles / Q000737:chemistry*; D009824:Ointments; D012867:Skin / Q000378:metabolism*; D014885:Waxes / Q000737:chemistry*
chemical_list: D003358:Cosmetics; D008055:Lipids; D009824:Ointments; D014885:Waxes; C038228:beeswax
keywords: beeswax; clinical efficacy; lipid nanoparticles; rheology; skin barrier function