title: Metal induced changes in trivalent chromium resistant Alcaligenes faecalis VITSIM2.
abstract: The changes induced in bacterial strains under stress conditions provide an insight into metal resistance strategies. Trivalent chromium resistant bacterium were isolated and identified by 16S rRNA gene sequencing and designated as Alcaligenes faecalis VITSIM2. The growth pattern was monitored. The organism also showed resistance to copper, cadmium, and certain antibiotics. The differentially expressed proteins in SDS PAGE were identified by mass spectrometry as flagellin and 50S ribosomal L36 protein. The morphological changes were identified by scanning electron microscopy. The changes in the cell wall content were estimated by peptidoglycan analysis and transformation of phosphates was detected by31P NMR. Flow cytometry was employed to measure the membrane integrity, esterase activity and intracellular pH. In conclusion spectrum of proteomic, physiological, and morphological alterations was observed that aid the organism to overcome chromium stress.
authors.affiliation: School of Bioscience and Technology, VIT University, Vellore-632014, India.
authors.forename: Shiny C
authors.initials: SC
authors.lastname: Matilda
authors.affiliation: School of Bioscience and Technology, VIT University, Vellore-632014, India.
authors.forename: Chittibabu
authors.initials: C
authors.lastname: Shanthi
mesh_terms: D042481:Alcaligenes faecalis / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D002857:Chromium / Q000494:pharmacology*; D004269:DNA, Bacterial; D004950:Esterases / Q000378:metabolism; D005798:Genes, Bacterial; D008670:Metals / Q000378:metabolism*; D010457:Peptidoglycan / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008670:Metals; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D012987:Soil; D002857:Chromium; D004950:Esterases
keywords: Alcaligenes faecalis; flagellin; peptidoglycan; trivalent chromium