title: [High glucose reduced the repair function of kidney stem cells conditional medium to the hypoxia-injured renal tubular epithelium cells].
abstract: To evaluate the impacts of high glucose on the repair function of kidney stem cells (KSC) conditional medium to the hypoxia-injured renal tubular epithelium cells (RTEC).
  
  
  
  KSC were isolated from the renal papilla in 4-week-Sprague-Dawley rats. The KSC were pretreated in media with high glucose (30 mmol/L) or with normal glucose (5.6 mmol/L), respectively. The supernatants of the pre-treated KSC were collected as the conditional media. The hypoxia/reoxygenation (H/R) model of rat RTEC was established using the NRK-52E cell line. The effects of KSC conditional media on the H/R RTEC were investigated.
  
  
  
  (1) The best H/R model of RTEC was established using hypoxia for 4 h and reoxygenation 2 h. (2) After hypoxia, the early and late cell apoptosis rates of the H/R RTEC were increased. The H/R RTEC were co-cultured with KSC conditional media for 12 h and 24 h, respectively. The H/R RTEC were co-cultured with DMEM/F12 as a control group. The cell apoptosis rate of H/R RTEC was lower after co-cultured with KSC conditional media (P<0.01), and the cell apoptosis rate of H/R RTEC in high glucose group was much higher than that in normal glucose group after co-cultured 24 h (P=0.02). (3) After hypoxia, the lactic dehydrogenase (LDH) and malondialdehyde (MDA) levels of the H/R RTEC supernatant were increased, and the superoxide dismutase (SOD) level decreased. The LDH and MDA levels were lower and the SOD level was higher after co-cultured with KSC conditional media for 12 h and 24 h, respectively (P<0.01). The LDH and MDA levels of H/R RTEC supernatant were much higher in the high glucose group than in the normal glucose group (P<0.05), and the SOD level of H/R RTEC supernatant was much lower in the high glucose group than in the normal glucose group (P<0.01).
  
  
  
  KSC conditional media could repair the H/R injury of RTEC. The effects were mainly by inhibiting cell apoptosis, and reducing oxidative stress; the anti-cell apoptosis ability and the anti-oxidative stress capacity of the conditional medium were reduced after KSC were pre-treated with high glucose.
authors.affiliation: Department of Geriatric Nephrology, PLA General Hospital, State Key Laboratory of Kidney Disease, Beijing 100853, China.
authors.forename: G
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of Geriatric Nephrology, PLA General Hospital, State Key Laboratory of Kidney Disease, Beijing 100853, China.
authors.forename: Q L
authors.initials: QL
authors.lastname: Cheng
authors.affiliation: Department of Geriatric Endocrinology, PLA General Hospital, Beijing 100853, China.
authors.forename: C L
authors.initials: CL
authors.lastname: Li
authors.affiliation: Department of Geriatric Nephrology, PLA General Hospital, State Key Laboratory of Kidney Disease, Beijing 100853, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Jia
authors.affiliation: Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing 100850, China.
authors.forename: W
authors.initials: W
authors.lastname: Yue
authors.affiliation: Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing 100850, China.
authors.forename: X T
authors.initials: XT
authors.lastname: Pei
authors.affiliation: Department of Geriatric Nephrology, PLA General Hospital, State Key Laboratory of Kidney Disease, Beijing 100853, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Geriatric Nephrology, PLA General Hospital, State Key Laboratory of Kidney Disease, Beijing 100853, China.
authors.forename: J H
authors.initials: JH
authors.lastname: Zhao
authors.affiliation: Department of Geriatric Nephrology, PLA General Hospital, State Key Laboratory of Kidney Disease, Beijing 100853, China.
authors.forename: J
authors.initials: J
authors.lastname: Du
authors.affiliation: Department of Geriatric Nephrology, PLA General Hospital, State Key Laboratory of Kidney Disease, Beijing 100853, China.
authors.forename: Q G
authors.initials: QG
authors.lastname: Ao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line / Q000187:drug effects / Q000502:physiology; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism* / Q000502:physiology*; D005947:Glucose / Q000494:pharmacology* / Q000633:toxicity; D000860:Hypoxia / Q000503:physiopathology; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism* / Q000502:physiology*; D007684:Kidney Tubules / Q000166:cytology* / Q000187:drug effects* / Q000503:physiopathology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase; D005947:Glucose