title: Induction of Diverse Bioactive Secondary Metabolites from the Mangrove Endophytic Fungus Trichoderma sp. (Strain 307) by Co-Cultivation with Acinetobacter johnsonii (Strain B2).
abstract: Two new sesquiterpenes, microsphaeropsisin B (1) and C (2), and two new de-O-methyllasiodiplodins, (3R, 7R)-7-hydroxy-de-O-methyllasiodiplodin (4) and (3R)-5-oxo-de-O-methyllasiodiplodin (5), together with one new natural product (6) and twelve known compounds (3,7-17), were isolated from the co-cultivation of mangrove endophytic fungusTrichodermasp. 307 and aquatic pathogenic bacteriumAcinetobacter johnsoniiB2. Their structures, including absolute configurations, were elucidated by extensive analysis of spectroscopic data, electronic circular dichroism, Mo₂(AcO)₄-induced circular dichroism, and comparison with reported data. All of the isolated compounds were tested for their α-glucosidase inhibitory activity and cytotoxicity. New compounds4and5exhibited potent α-glucosidase inhibitory activity with IC50values of 25.8 and 54.6 µM, respectively, which were more potent than the positive control (acarbose, IC50= 703.8 µM). The good results of the tested bioactivity allowed us to explore α-glucosidase inhibitors in lasiodiplodins.
authors.affiliation: School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510006, China. zhanglhn@mail2.sysu.edu.cn.
authors.forename: Liuhong
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510006, China. shah_iram2000@yahoo.com.
authors.forename: Shah Iram
authors.initials: SI
authors.lastname: Niaz
authors.affiliation: School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510006, China. dilfarazkhan@gu.edu.pk.
authors.forename: Dilfaraz
authors.initials: D
authors.lastname: Khan
authors.affiliation: Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510275, China. wangzlucky@163.com.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510275, China. zhuyongh@mail.sysu.edu.cn.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Instrumental Analysis & Research Center, Sun Yat-Sen University, Guangzhou 510275, China. zhouhy@mail.sysu.edu.cn.
authors.forename: Haiyun
authors.initials: H
authors.lastname: Zhou
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China. ceslyc@mail.sysu.edu.cn.
authors.forename: Yongcheng
authors.initials: Y
authors.lastname: Lin
authors.affiliation: School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510006, China. lijing356@mail.sysu.edu.cn.|South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Sun Yat-Sen University, Guangzhou 510275, China. lijing356@mail.sysu.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510006, China. cesllan@mail.sysu.edu.cn.|South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Sun Yat-Sen University, Guangzhou 510275, China. cesllan@mail.sysu.edu.cn.
authors.forename: Lan
authors.initials: L
authors.lastname: Liu
mesh_terms: D000150:Acinetobacter / Q000737:chemistry* / Q000378:metabolism*; D001688:Biological Products / Q000737:chemistry / Q000378:metabolism*; D002942:Circular Dichroism / Q000379:methods; D060026:Endophytes / Q000737:chemistry / Q000378:metabolism*; D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry / Q000378:metabolism; D020128:Inhibitory Concentration 50; D018942:Macrolides / Q000737:chemistry / Q000378:metabolism; D012717:Sesquiterpenes / Q000737:chemistry / Q000378:metabolism; D014242:Trichoderma / Q000737:chemistry* / Q000378:metabolism*; D015025:Zearalenone / Q000031:analogs & derivatives / Q000737:chemistry; D000520:alpha-Glucosidases / Q000737:chemistry / Q000378:metabolism
chemical_list: D001688:Biological Products; D065089:Glycoside Hydrolase Inhibitors; D018942:Macrolides; D012717:Sesquiterpenes; C558290:de-O-methyllasiodiplodin; C417552:lasiodiplodin; D015025:Zearalenone; D000520:alpha-Glucosidases
keywords: co-cultivation; lasiodiplodin; mangrove endophytic fungus; sesquiterpene; α-glucosidase inhibitor