title: Gene expressions levels of 14-3-3a, NKCCla, APO-14, and Na+-K+-ATPaseβ in gill tissue of Mugil cephalus acclimated to low salinity.
abstract: Fishes adapt to salinity changes primarily through osmotic pressure regulation, a process often associated with several genes, including 14-3-3a, NKCCla, APO-14, and Na+-K+-ATPaseβ. The present study investigated the differential expression of genes 14-3-3a, NKCCla, APO-14, and Na+-K+-ATPaseβ in the gill tissue of Mugil cephalus acclimated to low salinity. Susceptibility relationships between the four gene expressions levels and salinity were detected and analyzed using polymerase chain reaction-restriction fragment length polymorphism. Homology analysis results indicated significant differences in the correlation between gene expression and salinity. Under low-salt conditions, expression levels for genes Na+-K+-ATPaseβ and NKCC1a were significantly elevated (P < 0.05), whereas those of genes 14-3-3a and APO-14 were significantly reduced (P < 0.05). Thus, when compared to 14-3-3a and APO-14, Na+-K+-ATPaseβ, and NKCC1a may be better suited to promoting the development of osmotic-regulation mechanisms and increased resistance to environmental stress under low-salt conditions. Furthermore, Na+-K+-ATPaseβ and NKCC1a were identified as suitable potential molecular biomarkers for regulating and controlling genes in low-salinity aquatic environments.
authors.affiliation: East China Sea Fisheries Research Institute Chinese Academy of Fishery Sciences, Shanghai, China.
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.affiliation: East China Sea Fisheries Research Institute Chinese Academy of Fishery Sciences, Shanghai, China jiangrose73@sina.com.
authors.forename: M
authors.initials: M
authors.lastname: Jiang
authors.affiliation: East China Sea Fisheries Research Institute Chinese Academy of Fishery Sciences, Shanghai, China.
authors.forename: X Q
authors.initials: XQ
authors.lastname: Shen
mesh_terms: D048948:14-3-3 Proteins / Q000235:genetics; D000222:Adaptation, Physiological; D000818:Animals; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D005399:Fishes / Q000235:genetics* / Q000254:growth & development; D015870:Gene Expression; D005786:Gene Expression Regulation; D005880:Gills / Q000378:metabolism*; D054712:Salinity
chemical_list: D048948:14-3-3 Proteins; D029941:Fish Proteins