title: Metal-Organic Framework Nanomaterials as Novel Signal Probes for Electron Transfer Mediated Ultrasensitive Electrochemical Immunoassay.
abstract: A novel and simple electrochemical immunoassay for C-reactive protein was developed using metal-organic frameworks (Au-MOFs) as signal unit. In this study, we found MOFs could be used as signal probe. And this new class of signal probe differs from traditional probe. The signal of the copper ions (Cu2+) from MOFs could be directly detected without acid dissolution and preconcentration, which would greatly simplify the detection steps and reduce the detection time. Moreover, MOFs contain large amounts of Cu2+ions, providing high electrochemical signals. Our report represents the first example of using MOFs themselves as electrochemical signal probe for biosensors. Platinum nanoparticle modified covalent organic frameworks (Pt-COFs) with high electronic conductivity was employed as the substrate, which is the first time demonstrating the use of Pt-COFs for electrochemical immunoassay. Under the optimized experimental conditions, the proposed sensing strategy provides a linear dynamic ranging from 1 to 400 ng/mL. A detection limit of 0.2 ng/mL was obtained, indicating an improved analytical performance. With these merits, this stable, simple, low-cost, sensitive and selective electrochemical immunoassay shows promise for applications in the point-of-care diagnostics of dieses and environmental monitoring.
authors.affiliation: College of Chemistry and Chemical Engineering, Yunnan Normal University , Yunnan, Kunming, 650092, People's Republic of China.
authors.forename: Ting-Zhi
authors.initials: TZ
authors.lastname: Liu
authors.affiliation: College of Chemistry and Chemical Engineering, Yunnan Normal University , Yunnan, Kunming, 650092, People's Republic of China.
authors.forename: Rong
authors.initials: R
authors.lastname: Hu
authors.affiliation: College of Chemistry and Chemical Engineering, Yunnan Normal University , Yunnan, Kunming, 650092, People's Republic of China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, Yunnan Normal University , Yunnan, Kunming, 650092, People's Republic of China.
authors.forename: Kun-Lei
authors.initials: KL
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, Yunnan Normal University , Yunnan, Kunming, 650092, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Molecular Engineering for Theronastics, Hunan University , Changsha, 410082, People's Republic of China.
authors.forename: Xiao-Bing
authors.initials: XB
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, Yunnan Normal University , Yunnan, Kunming, 650092, People's Republic of China.
authors.forename: Ru-Yan
authors.initials: RY
authors.lastname: Bai
authors.affiliation: College of Chemistry and Chemical Engineering, Yunnan Normal University , Yunnan, Kunming, 650092, People's Republic of China.
authors.forename: Delei
authors.initials: D
authors.lastname: Li
authors.affiliation: College of Chemistry and Chemical Engineering, Yunnan Normal University , Yunnan, Kunming, 650092, People's Republic of China.
authors.forename: Yun-Hui
authors.identifier: 0000-0001-9235-7274
authors.initials: YH
authors.lastname: Yang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D002097:C-Reactive Protein / Q000032:analysis*; D003300:Copper / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D004579:Electron Transport; D006046:Gold / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D000073396:Metal-Organic Frameworks / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010984:Platinum / Q000737:chemistry
chemical_list: D000073396:Metal-Organic Frameworks; D010984:Platinum; D006046:Gold; D003300:Copper; D002097:C-Reactive Protein