title: Nasal Polyp-Derived Mesenchymal Stromal Cells Exhibit Lack of Immune-Associated Molecules and High Levels of Stem/Progenitor Cells Markers.
abstract: Mesenchymal stromal cells (MSCs) are considered adult progenitor stem cells and have been studied in a multitude of tissues. In this context, the microenvironment of nasal polyp tissue has several inflammatory cells, but their stroma compartment remains little elucidated. Hence, we isolated MSCs from nasal polyps Polyp-MSCs (PO-MSCs) and compared their molecular features and gene expression pattern with bone marrow-derived MSCs (BM-MSCs). Initially, both PO-MSCs and BM-MSCs were isolated, cultivated, and submitted to morphologic, differentiation, phenotypic, immunosuppressive, and gene expression assays. Compared to BM-MSCs, PO-MSCs showed normal morphology and similar osteogenic/adipogenic differentiation potential, but their immunophenotyping showed lack of immune-associated molecules (e.g., CD117, HLA-DR, PDL-1, and PDL-2), which was linked with less immunoregulatory abilities such as (i) inhibition of lymphocytes proliferation and (ii) regulatory T cell expansion. Furthermore, we detected in the PO-MSCs a distinct gene expression profile in comparison with BM-MSCs. PO-MSC expressed higher levels of progenitor stem cells specific markers (e.g., CD133 and ABCB1), while BM-MSCs showed elevated expression of cytokines and growth factors (e.g., FGF10, KDR, and GDF6). The gene ontology analysis showed that the differentially modulated genes in PO-MSC were related with matrix remodeling process and hexose and glucose transport. For BM-MSCs, the highly expressed genes were associated with behavior, angiogenesis, blood vessel morphogenesis, cell-cell signaling, and regulation of response to external stimulus. Thus, these results suggest that PO-MSCs, while sharing similar aspects with BM-MSCs, express a different profile of molecules, which presumably can be implicated in the development of nasal polyp tissue.
authors.affiliation: ENT Research Laboratory, Department of Otolaryngology-Head and Neck Surgery, Federal University of São Paulo , São Paulo , Brazil.
authors.forename: Pedro Wey Barbosa
authors.initials: PW
authors.lastname: de Oliveira
authors.affiliation: ENT Research Laboratory, Department of Otolaryngology-Head and Neck Surgery, Federal University of São Paulo , São Paulo , Brazil.
authors.forename: Rogério
authors.initials: R
authors.lastname: Pezato
authors.affiliation: ENT Research Laboratory, Department of Otolaryngology-Head and Neck Surgery, Federal University of São Paulo , São Paulo , Brazil.
authors.forename: Juan Sebastian Henao
authors.initials: JS
authors.lastname: Agudelo
authors.affiliation: Department Biomedical Sciences, University of Antwerp, PPES Lab Proteinchemistry, Proteomics Epigenetic Signaling , Wilrijk , Belgium.
authors.forename: Claudina Angela
authors.initials: CA
authors.lastname: Perez-Novo
authors.affiliation: Department Biomedical Sciences, University of Antwerp, PPES Lab Proteinchemistry, Proteomics Epigenetic Signaling , Wilrijk , Belgium.
authors.forename: Wim Vanden
authors.initials: WV
authors.lastname: Berghe
authors.affiliation: Department of Medicine, Nephrology Division, Federal University of São Paulo, São Paulo, Brazil; Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Niels Olsen
authors.initials: NO
authors.lastname: Câmara
authors.affiliation: Department Biomedical Sciences, University of Antwerp, PPES Lab Proteinchemistry, Proteomics Epigenetic Signaling , Wilrijk , Belgium.
authors.forename: Danilo Candido
authors.initials: DC
authors.lastname: de Almeida
authors.affiliation: ENT Research Laboratory, Department of Otolaryngology-Head and Neck Surgery, Federal University of São Paulo , São Paulo , Brazil.
authors.forename: Luís Carlos
authors.initials: LC
authors.lastname: Gregorio
keywords: gene expression; immunoregulation; mesenchymal stromal cells; nasal polyposis; stem cells