title: Effect of BjcuL, a lectin isolated from Bothrops jararacussu, on human peripheral blood mononuclear cells.
abstract: BjcuL is a C-type lectin with specificity for the binding of β-d-galactose units isolated from Bothrops jararacussu venom. It triggers cellular infiltration in post capillary venules, increases edema and vascular permeability in murine models, contributes to in vitro neutrophil activation and modulates macrophage functional activation towards an M1 state. The purpose of this study was to investigate the effect of BjcuL on human peripheral blood mononuclear cells (PBMCs) activation with a focus on PBMCs proliferation and inflammatory mediators release. Results showed that BjcuL is not toxic to PBMCs, that BjcuL inhibits PBMCs proliferation and that it stimulates PBMCs to produce superoxide anion and hydrogen peroxide, primarily via lymphocyte stimulation, but does not stimulate the production of nitric oxide and PGE2. These results demonstrate that BjcuL has an immunomodulatory effect on PBMCs. Further studies are needed to confirm the immunomodulatory effect of BjcuL, to elucidate the molecular mechanisms of action responsible for its effects and to determine its potential application as an immunopharmacological and biotechnological tool.
authors.affiliation: Laboratório de Imunologia Celular Aplicada à Saúde, Fundação Oswaldo Cruz, FIOCRUZ Rondônia, Porto Velho, RO, Brazil.
authors.forename: Weverson Luciano
authors.initials: WL
authors.lastname: Pires
authors.affiliation: Laboratório de Imunologia Celular Aplicada à Saúde, Fundação Oswaldo Cruz, FIOCRUZ Rondônia, Porto Velho, RO, Brazil.
authors.forename: Onassis Boeri
authors.initials: OB
authors.lastname: de Castro
authors.affiliation: Laboratório de Imunologia Celular Aplicada à Saúde, Fundação Oswaldo Cruz, FIOCRUZ Rondônia, Porto Velho, RO, Brazil; Centro de Estudos de Biomoléculas Aplicadas à Saúde (CEBio), Fundação Oswaldo Cruz, FIOCRUZ Rondônia e Departamento de Medicina, Universidade Federal de Rondônia, UNIR, Porto Velho, RO, Brazil.
authors.forename: Anderson Makoto
authors.initials: AM
authors.lastname: Kayano
authors.affiliation: Laboratório de Imunologia Celular Aplicada à Saúde, Fundação Oswaldo Cruz, FIOCRUZ Rondônia, Porto Velho, RO, Brazil.
authors.forename: Sulamita
authors.initials: S
authors.lastname: da Silva Setúbal
authors.affiliation: Laboratório de Imunologia Celular Aplicada à Saúde, Fundação Oswaldo Cruz, FIOCRUZ Rondônia, Porto Velho, RO, Brazil.
authors.forename: Adriana Silva
authors.initials: AS
authors.lastname: Pontes
authors.affiliation: Laboratório de Imunologia Celular Aplicada à Saúde, Fundação Oswaldo Cruz, FIOCRUZ Rondônia, Porto Velho, RO, Brazil.
authors.forename: Neriane Monteiro
authors.initials: NM
authors.lastname: Nery
authors.affiliation: Laboratório de Imunologia Celular Aplicada à Saúde, Fundação Oswaldo Cruz, FIOCRUZ Rondônia, Porto Velho, RO, Brazil.
authors.forename: Mauro Valentino
authors.initials: MV
authors.lastname: Paloschi
authors.affiliation: Laboratório de Engenharia de Anticorpos, Fundação Oswaldo Cruz, FIOCRUZ Rondônia, Porto Velho, RO, Brazil.
authors.forename: Soraya
authors.initials: S
authors.lastname: Dos Santos Pereira
authors.affiliation: Centro de Estudos de Biomoléculas Aplicadas à Saúde (CEBio), Fundação Oswaldo Cruz, FIOCRUZ Rondônia e Departamento de Medicina, Universidade Federal de Rondônia, UNIR, Porto Velho, RO, Brazil.
authors.forename: Rodrigo Guerino
authors.initials: RG
authors.lastname: Stábeli
authors.affiliation: Laboratório de Engenharia de Anticorpos, Fundação Oswaldo Cruz, FIOCRUZ Rondônia, Porto Velho, RO, Brazil; Centro de Pesquisa em Medicina Tropical, Porto Velho, RO, Brazil.
authors.forename: Carla Freire Celedônio
authors.initials: CFC
authors.lastname: Fernandes
authors.affiliation: Centro de Estudos de Biomoléculas Aplicadas à Saúde (CEBio), Fundação Oswaldo Cruz, FIOCRUZ Rondônia e Departamento de Medicina, Universidade Federal de Rondônia, UNIR, Porto Velho, RO, Brazil.
authors.forename: Andreimar Martins
authors.initials: AM
authors.lastname: Soares
authors.affiliation: Laboratório de Imunologia Celular Aplicada à Saúde, Fundação Oswaldo Cruz, FIOCRUZ Rondônia, Porto Velho, RO, Brazil; Centro de Estudos de Biomoléculas Aplicadas à Saúde (CEBio), Fundação Oswaldo Cruz, FIOCRUZ Rondônia e Departamento de Medicina, Universidade Federal de Rondônia, UNIR, Porto Velho, RO, Brazil. Electronic address: juliana.zuliani@fiocruz.br.
authors.forename: Juliana Pavan
authors.initials: JP
authors.lastname: Zuliani
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003435:Crotalid Venoms / Q000633:toxicity*; D051545:Cyclooxygenase 1 / Q000235:genetics; D051546:Cyclooxygenase 2 / Q000235:genetics; D015232:Dinoprostone / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006388:Hemagglutinins / Q000378:metabolism; D006801:Humans; D007155:Immunologic Factors / Q000633:toxicity*; D037181:Lectins, C-Type; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: C498078:BJcuL lectin, Bothrops jararacussu; D003435:Crotalid Venoms; D006388:Hemagglutinins; D007155:Immunologic Factors; D037181:Lectins, C-Type; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; C496511:PTGS1 protein, human; C496540:PTGS2 protein, human; D015232:Dinoprostone
keywords: Lectin; Mediators; PBMCs; Snake venom