title: A rapid and robust UHPLC-DAD method for the quantification of amphotericin B in human plasma.
abstract: Amphotericin B is an antifungal drug widely used in Intensive Care Units. Therapeutic drug monitoring (TDM) of amphotericin B is recommended for the assessment of toxicity surveillance and treatment optimization. In this paper we described the development and validation of a new Ultra High Performance Liquid Chromatography coupled to Diode Array Detection (UHPLC-DAD) method for the quantification of Amphotericin B in 200μL human plasma over a wide range of concentrations (0.125-10mg/L). The new method has been validated following international guidelines on bioanalytical method validation and showed high selectivity, high accuracy and precision and high process efficiency. The new UHPLC-DAD method that we describe is robust, rapid, cost effective and suitable for application to the routine TDM analyses.
authors.affiliation: Clinical Pathology Laboratory Unit, Istituto Giannina Gaslini, Genoa, Italy.
authors.forename: Sebastiano
authors.initials: S
authors.lastname: Barco
authors.affiliation: Clinical Pathology Laboratory Unit, Istituto Giannina Gaslini, Genoa, Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Zunino
authors.affiliation: Department of Medical Sciences, University of Turin, Laboratory of Clinical Pharmacology and Pharmacogenetics, Amedeo di Savoia Hospital, Turin, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: D'Avolio
authors.affiliation: Clinical Pathology Laboratory Unit, Istituto Giannina Gaslini, Genoa, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Barbagallo
authors.affiliation: Clinical Pathology Laboratory Unit, Istituto Giannina Gaslini, Genoa, Italy.
authors.forename: Angelo
authors.initials: A
authors.lastname: Maffia
authors.affiliation: Clinical Pathology Laboratory Unit, Istituto Giannina Gaslini, Genoa, Italy.
authors.forename: Gino
authors.initials: G
authors.lastname: Tripodi
authors.affiliation: Infectious Disease Unit, Istituto Giannina Gaslini, Genoa, Italy.
authors.forename: Elio
authors.initials: E
authors.lastname: Castagnola
authors.affiliation: Clinical Pathology Laboratory Unit, Istituto Giannina Gaslini, Genoa, Italy. Electronic address: giulianacangemi@ospedale-gaslini.ge.it.
authors.forename: Giuliana
authors.initials: G
authors.lastname: Cangemi
mesh_terms: D000293:Adolescent; D000666:Amphotericin B / Q000097:blood* / Q000737:chemistry*; D000935:Antifungal Agents / Q000097:blood / Q000737:chemistry; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016903:Drug Monitoring / Q000379:methods; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D010949:Plasma / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D000935:Antifungal Agents; D000666:Amphotericin B
keywords: Amphotericin B; UHPLC