title: First isolation and characterization of pteropine orthoreoviruses in fruit bats in the Philippines.
abstract: Pteropine orthoreovirus (PRV) causes respiratory tract illness (RTI) in humans. PRVs were isolated from throat swabs collected from 9 of 91 wild bats captured on the Mindanao Islands, The Philippines, in 2013. The nucleic acid sequence of the whole genome of each of these isolates was determined. Phylogenetic analysis based on predicted amino acid sequences indicated that the isolated PRVs were novel strains in which re-assortment events had occurred in the viral genome. Serum specimens collected from 76 of 84 bats were positive for PRV-neutralizing antibodies suggesting a high prevalence of PRV in wild bats in the Philippines. The bat-borne PRVs isolated in the Philippines were characterized in comparison to an Indonesian PRV isolate, Miyazaki-Bali/2007 strain, recovered from a human patient, revealing that the Philippine bat-borne PRVs had similar characteristics in terms of antigenicity to those of the Miyazaki-Bali/2007 strain, but with a slight difference (e.g., growth capacity in vitro). The impact of the Philippine bat-borne PRVs should be studied in human RTI cases in the Philippines.
authors.affiliation: Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo, 208-0011, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Taniguchi
authors.affiliation: Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary MedicineYamaguchi University, 1677-1 Yoshida, Yamaguchi, 753-8515, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Maeda
authors.affiliation: Department of Veterinary Microbiology, Graduate School of Agricultural and Life SciencesThe University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
authors.forename: Taisuke
authors.initials: T
authors.lastname: Horimoto
authors.affiliation: College of Veterinary Medicine, University of the Philippine Los Baños, Los Baños, 4031, Laguna, Philippines.
authors.forename: Joseph S
authors.initials: JS
authors.lastname: Masangkay
authors.affiliation: University of the Philippines Mindanao, Bago-Oshiro, Davao, Philippines.
authors.forename: Roberto
authors.initials: R
authors.lastname: Puentespina
authors.affiliation: Museum of Natural History, University of the Philippines Los Baños, Los Baños, 4031, Laguna, Philippines.
authors.forename: James
authors.initials: J
authors.lastname: Alvarez
authors.affiliation: Museum of Natural History, University of the Philippines Los Baños, Los Baños, 4031, Laguna, Philippines.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Eres
authors.affiliation: Museum of Natural History, University of the Philippines Los Baños, Los Baños, 4031, Laguna, Philippines.
authors.forename: Edison
authors.initials: E
authors.lastname: Cosico
authors.affiliation: Department of Pathology, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo, 208-0011, Japan.
authors.forename: Noriyo
authors.initials: N
authors.lastname: Nagata
authors.affiliation: Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo, 208-0011, Japan.
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Egawa
authors.affiliation: Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo, 208-0011, Japan.
authors.forename: Harpal
authors.initials: H
authors.lastname: Singh
authors.affiliation: Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo, 208-0011, Japan.
authors.forename: Aiko
authors.initials: A
authors.lastname: Fukuma
authors.affiliation: Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo, 208-0011, Japan.
authors.forename: Tomoki
authors.initials: T
authors.lastname: Yoshikawa
authors.affiliation: Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo, 208-0011, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Tani
authors.affiliation: Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo, 208-0011, Japan.
authors.forename: Shuetsu
authors.initials: S
authors.lastname: Fukushi
authors.affiliation: Research and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo, 183-8509, Japan.
authors.forename: Shinobu
authors.initials: S
authors.lastname: Tsuchiaka
authors.affiliation: Research and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo, 183-8509, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Omatsu
authors.affiliation: Research and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo, 183-8509, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizutani
authors.affiliation: Laboratory of Veterinary Pathology, School of Veterinary Medicine Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa, 252-5201, Japan.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Une
authors.affiliation: Department of Animal Risk Management, Chiba Institute of Science, Choshi, Chiba, 288-0025, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yoshikawa
authors.affiliation: Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo, 208-0011, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shimojima
authors.affiliation: Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo, 208-0011, Japan. msaijo@niid.go.jp.|Department of Virology 1, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan. msaijo@niid.go.jp.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Saijo
authors.affiliation: Department of Biomedical Science, Graduate School of Agricultural and Life SciencesThe University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kyuwa
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000821:virology; D057134:Antibodies, Neutralizing / Q000097:blood; D002685:Chiroptera / Q000276:immunology / Q000821:virology*; D016679:Genome, Viral; D006801:Humans; D007214:Indonesia / Q000453:epidemiology; D018165:Orthoreovirus / Q000145:classification* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D010679:Philippines / Q000453:epidemiology; D012367:RNA, Viral / Q000235:genetics; D012088:Reoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology
chemical_list: D057134:Antibodies, Neutralizing; D012367:RNA, Viral