title: Genomic sequencing of a strain of Acinetobacter baumannii and potential mechanisms to antibiotics resistance.
abstract: Acinetobacter baumannii has been becoming a great challenge to clinicians due to their resistance to almost all available antibiotics. In this study, we sequenced the genome from a multiple antibiotics resistant Acinetobacter baumannii stain which was named A. baumannii-1isolated from China by SMRT sequencing technology to explore its potential mechanisms to antibiotic resistance. We found that several mechanisms might contribute to the antibiotic resistance of Acinetobacter baumannii. Specifically, we found that SNP in genes associated with nucleotide excision repair and ABC transporter might contribute to its resistance to multiple antibiotics; we also found that specific genes associated with bacterial DNA integration and recombination, DNA-mediated transposition and response to antibiotics might contribute to its resistance to multiple antibiotics; Furthermore, specific genes associated with penicillin and cephalosporin biosynthetic pathway and specific genes associated with CHDL and MBL β-lactamase genes might contribute to its resistance to multiple antibiotics. Thus, the detailed mechanisms by which Acinetobacter baumannii show extensive resistance to multiple antibiotics are very complicated. Such a study might be helpful to develop new strategies to control Acinetobacter baumannii infection.
authors.affiliation: Department of Infectious Disease, The First Affiliated Hospital of Anhui Medical University, China; Department of Molecular Biology, Institute of Bacterium Resistance, Anhui Medical University, China; Department of Molecular Biology, Anhui Center for Surveillance of Bacterial Resistance, Hefei, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Clinical Medicine, Xiangya Hospital Central South University, Changsha, China.
authors.forename: Hongru
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Surgery, University of Missouri School of Medicine, Columbia, MO, USA.
authors.forename: Ziwen
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Department of Surgery, University of Missouri School of Medicine, Columbia, MO, USA.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Wakefield
authors.affiliation: Department of Surgery, University of Missouri School of Medicine, Columbia, MO, USA; Department of Microbiology, Immunology & Pathology, Des Moines University College of Osteopathic Medicine, Des Moines, IA, USA. Electronic address: yujiang.fang@dmu.edu.
authors.forename: Yujiang
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Department of Infectious Disease, The First Affiliated Hospital of Anhui Medical University, China; Department of Molecular Biology, Institute of Bacterium Resistance, Anhui Medical University, China; Department of Molecular Biology, Anhui Center for Surveillance of Bacterial Resistance, Hefei, China. Electronic address: yeying2@139.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ye
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D000151:Acinetobacter Infections / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D015780:Carbapenems / Q000494:pharmacology; D004260:DNA Repair; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D016680:Genome, Bacterial; D006801:Humans; D054643:INDEL Mutation; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism
chemical_list: D018528:ATP-Binding Cassette Transporters; D000900:Anti-Bacterial Agents; D015780:Carbapenems; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D001618:beta-Lactamases
keywords: Acinectobacter baumannii; Bacterium; Genome