title: ABC-Transporter Mediated Sterol Export from Cells Using Radiolabeled Sterols.
abstract: Cholesterol export from cells to extracellular acceptors represents the first step of the reverse cholesterol transport process and is an essential part of the multifaceted pathway for cells to control their cholesterol levels. Malfunction of this pathway leads to cholesterol accumulation in cells such as macrophages, which can form the basis of conditions like atherosclerosis. A number of ATP-binding cassette (ABC) transporters, namely ABCA1, ABCA7, ABCG1, and ABCG4, play an essential role in this process. In this chapter, we describe methods utilizing radiolabeled sterols for measuring ABC-transporter mediated sterol export, utilizing endogenously expressed transporters as well as overexpression systems.
authors.affiliation: Faculty of Pharmacy, The University of Sydney, Pharmacy Bank Building A15, Camperdown, Sydney, NSW, 2006, Australia.
authors.forename: Alryel
authors.initials: A
authors.lastname: Yang
authors.affiliation: Faculty of Pharmacy, The University of Sydney, Pharmacy Bank Building A15, Camperdown, Sydney, NSW, 2006, Australia. Ingrid.gelissen@sydney.edu.au.
authors.forename: Ingrid C
authors.initials: IC
authors.lastname: Gelissen
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D050197:Atherosclerosis / Q000235:genetics / Q000378:metabolism; D001693:Biological Transport, Active / Q000502:physiology; D016466:CHO Cells; D002784:Cholesterol / Q000235:genetics / Q000378:metabolism*; D003412:Cricetulus; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D000074084:THP-1 Cells
chemical_list: D018528:ATP-Binding Cassette Transporters; D002784:Cholesterol
keywords: ABC-transporter; ABCA1; ABCA7; ABCG1; ABCG4; Cholesterol export; Reverse cholesterol transport