title: Proteomic profiling of mature leaves from oil palm (Elaeis guineensis Jacq.).
abstract: Oil palm is one of the most productive oil bearing crops grown in Southeast Asia. Due to the dwindling availability of agricultural land and increasing demand for high yielding oil palm seedlings, clonal propagation is vital to the oil palm industry. Most commonly, leaf explants are used for in vitro micropropagation of oil palm and to optimize this process it is important to unravel the physiological and molecular mechanisms underlying somatic embryo production from leaves. In this study, a proteomic approach was used to determine protein abundance of mature oil palm leaves. To do this, leaf proteins were extracted using TCA/acetone precipitation protocol and separated by 2DE. A total of 191 protein spots were observed on the 2D gels and 67 of the most abundant protein spots that were consistently observed were selected for further analysis with 35 successfully identified using MALDI TOF/TOF MS. The majority of proteins were classified as being involved in photosynthesis, metabolism, cellular biogenesis, stress response, and transport. This study provides the first proteomic assessment of oil palm leaves in this important oil crop and demonstrates the successful identification of selected proteins spots using the Malaysian Palm Oil Board (MPOB) Elaeis guineensis EST and NCBI-protein databases. The MS data have been deposited in the ProteomeXchange Consortium database with the data set identifier PXD001307.
authors.affiliation: School of Biosciences, The University of Nottingham Malaysia Campus, Semenyih, Selangor Darul Ehsan, Malaysia.
authors.forename: Hooi Sin
authors.initials: HS
authors.lastname: Tan
authors.affiliation: Australia Research Council Centre of Excellence in Plant Energy Biology, Crawley, Western Australia, Australia.|School of Chemistry and Biochemistry, The University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: Richard P
authors.initials: RP
authors.lastname: Jacoby
authors.affiliation: Malaysian Palm Oil Board, Bandar Baru Bangi, Kajang, Selangor Darul Ehsan, Malaysia.
authors.forename: Meilina
authors.initials: M
authors.lastname: Ong-Abdullah
authors.affiliation: Australia Research Council Centre of Excellence in Plant Energy Biology, Crawley, Western Australia, Australia.|School of Chemistry and Biochemistry, The University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: Nicolas L
authors.initials: NL
authors.lastname: Taylor
authors.affiliation: School of Biosciences, Faculty of Science, Division of Animal Sciences, University of Nottingham, Nottingham, United Kingdom.
authors.forename: Susan
authors.initials: S
authors.lastname: Liddell
authors.affiliation: AAR-UNMC Biotechnology Research Centre (Advanced Agriecological Research Sdn. Bhd.), Semenyih, Selangor Darul Ehsan, Malaysia.
authors.forename: Wong Wei
authors.initials: WW
authors.lastname: Chee
authors.affiliation: School of Biosciences, The University of Nottingham Malaysia Campus, Semenyih, Selangor Darul Ehsan, Malaysia.
authors.forename: Chiew Foan
authors.initials: CF
authors.lastname: Chin
mesh_terms: D028023:Arecaceae / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D018515:Plant Leaves / Q000737:chemistry*; D010938:Plant Oils; D010940:Plant Proteins / Q000032:analysis*; D040901:Proteomics
chemical_list: D010938:Plant Oils; D010940:Plant Proteins
keywords: Elaeis guineensis; Leaf proteome; MALDI TOF/TOF; Oil Palm; Two-dimensional electrophoresis