title: Complete genome sequence of Amycolatopsis orientalis CPCC200066, the producer of norvancomycin.
abstract: Amycolatopsis orientalis CPCC200066 is an actinomycete exploited commercially in China for the production of norvancomycin, an important glycopeptide antibiotic structurally close to the well-known vancomycin. The availability of the complete genome sequence of CPCC200066 would greatly strengthen our understanding of the regulation pattern of norvancomycin biosynthesis and ultimately improve its production, as well as potentiate discoveries of novel bioactive compounds. Here we report the complete genome sequence of A. orientalis CPCC200066, a circular chromosome consisting of 9,490,992bp. Forty putative secondary metabolite biosynthetic gene clusters, including norvancomycin, were predicted, covering 20.3% of the whole genome. To facilitate genetic manipulation of this strain, an efficient transformation system was established by constructing a novel integrative vector pIMBT1, which could be transferred into CPCC200066 by electroporation with high efficiency. ΦBT1 attB sites were also identified in other known Amycolatopsis genomes, indicating pIMBT1's prospect to be a novel vector for genus Amycolatopsis.
authors.affiliation: Key Laboratory of Biotechnology of Antibiotics, The National Health and Family Planning Commission (NHFPC), Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Lei
authors.affiliation: Key Laboratory of Biotechnology of Antibiotics, The National Health and Family Planning Commission (NHFPC), Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biotechnology of Antibiotics, The National Health and Family Planning Commission (NHFPC), Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors.forename: Zhibo
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Biotechnology of Antibiotics, The National Health and Family Planning Commission (NHFPC), Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Biotechnology of Antibiotics, The National Health and Family Planning Commission (NHFPC), Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Immunology Program Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.affiliation: Key Laboratory of Biotechnology of Antibiotics, The National Health and Family Planning Commission (NHFPC), Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors.forename: Yunying
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Key Laboratory of Biotechnology of Antibiotics, The National Health and Family Planning Commission (NHFPC), Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. Electronic address: lifeiwang2002@hotmail.com.
authors.forename: Lifei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Biotechnology of Antibiotics, The National Health and Family Planning Commission (NHFPC), Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. Electronic address: binhong69@hotmail.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Hong
mesh_terms: D000192:Actinomycetales / Q000235:genetics* / Q000378:metabolism; D001482:Base Composition; D002874:Chromosome Mapping; D059646:Genome Size; D016680:Genome, Bacterial; D005810:Multigene Family; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000379:methods*; D014640:Vancomycin / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D014640:Vancomycin; C511195:norvancomycin
keywords: Amycolatopsis orientalis CPCC200066; Complete genome sequence; Integrative vector pIMBT1; Norvancomycin