title: Biofilm formation of Achromobacter xylosoxidans on contact lens.
abstract: Achromobacter spp. may contaminate lenses, lens cases, and contact lens solutions and cause ocular infections. The aim of this study was to investigate the possibility of isolated strain of Achromobacter xylosoxidans to form biofilm on the surface of soft contact lenses (CL), to quantify the production of the formed biofilm, and compare it with the reference strains (Pseudomonas aeruginosa, Staphylococcus aureus, and Haemophilus influenzae). Bacterial strain isolated from one contact lens case was identified as A. xylosoxidans using Vitek2 Automated System. Biofilm forming capacity of isolated strain of A. xylosoxidans and reference strains of P. aeruginosa, S. aureus, and H. influenzae on soft CL were analyzed by commonly used microtitre plate method. Our results showed that isolated strain of A. xylosoxidans was capable to form biofilm on the surface of soft contact lens. A. xylosoxidans was strong biofilm producer while all examined reference strains were moderate biofilm producers. A. xylosoxidans appears to be superior biofilm producer on soft CL compared to reference strains.
authors.affiliation: 1 Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade , Belgrade, Serbia.
authors.forename: Neda
authors.initials: N
authors.lastname: Konstantinović
authors.affiliation: 1 Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade , Belgrade, Serbia.
authors.forename: Ivana
authors.initials: I
authors.lastname: Ćirković
authors.affiliation: 1 Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade , Belgrade, Serbia.
authors.forename: Slobodanka
authors.initials: S
authors.lastname: Đukić
authors.affiliation: 2 Clinic of Eye Diseases, Clinical Center of Serbia , Belgrade, Serbia.
authors.forename: Vesna
authors.initials: V
authors.lastname: Marić
authors.affiliation: 3 Department of Microbiology and Immunology, Faculty of Pharmacy, University of Belgrade , Belgrade, Serbia.
authors.forename: Dragana D
authors.initials: DD
authors.lastname: Božić
mesh_terms: D042441:Achromobacter denitrificans / Q000502:physiology*; D018441:Biofilms / Q000254:growth & development*; D003263:Contact Lenses, Hydrophilic / Q000382:microbiology*; D013499:Surface Properties
keywords: Achromobacter xylosoxidans; biofilm; contact lens