title: Heterologous expression of Aspergillus aculeatus endo-polygalacturonase in Pichia pastoris by high cell density fermentation and its application in textile scouring.
abstract: Removal of non-cellulosic impurities from cotton fabric, known as scouring, by conventional alkaline treatment causes environmental problems and reduces physical strength of fabrics. In this study, an endo-polygalacturonase (EndoPG) from Aspergillus aculeatus produced in Pichia pastoris was evaluated for its efficiency as a bioscouring agent while most current bioscouring process has been performed using crude pectinase preparation.
  
  
  
  The recombinant EndoPG exhibited a specific activity of 1892.08 U/mg on citrus pectin under the optimal condition at 50 °C, pH 5.0 with a Vmaxand Kmof 65,451.35 μmol/min/mL and 15.14 mg/mL, respectively. A maximal activity of 2408.70 ± 26.50 U/mL in the culture supernatant was obtained by high cell density batch fermentation, equivalent to a 4.8 times greater yield than that from shake-flask culture. The recombinant enzyme was shown to be suitable for application as a bioscouring agent, in which the wettability of cotton fabric was increased by treatment with enzyme at 300 U/mL scouring solution at 40 °C, pH 5.0 for 1 h. The bio-scoured fabric has comparable wettability to that obtained by conventional chemical scouring, but has higher tensile strength.
  
  
  
  The work has demonstrated for the first time functions of A. aculeatus EndoPG on bioscouring in eco-textile processing. EndoPG alone was shown to possess effective scouring activity. High expression level and homogeneity could be achieved in bench-scale bioreactor.
authors.affiliation: Faculty of Biotechnology, Atmajaya Catholic University, Jl. Jend. Sudirman 51, Jakarta, 12930, Indonesia.
authors.forename: Dede
authors.initials: D
authors.lastname: Abdulrachman
authors.affiliation: Microbial Biotechnology and Biochemicals Research Unit, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Pahonyothin Rd, Khlong Luang, Patumthani, 12120, Thailand.
authors.forename: Paweena
authors.initials: P
authors.lastname: Thongkred
authors.affiliation: Microbial Biotechnology and Biochemicals Research Unit, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Pahonyothin Rd, Khlong Luang, Patumthani, 12120, Thailand.
authors.forename: Kanokarn
authors.initials: K
authors.lastname: Kocharin
authors.affiliation: Textile Laboratory, Polymers Research Unit, National Metal and Materials Technology Center, 114 Thailand Science Park, Pahonyothin Rd, Khlong Luang, Patumthani, 12120, Thailand.
authors.forename: Monthon
authors.initials: M
authors.lastname: Nakpathom
authors.affiliation: Textile Laboratory, Polymers Research Unit, National Metal and Materials Technology Center, 114 Thailand Science Park, Pahonyothin Rd, Khlong Luang, Patumthani, 12120, Thailand.
authors.forename: Buppha
authors.initials: B
authors.lastname: Somboon
authors.affiliation: Textile Laboratory, Polymers Research Unit, National Metal and Materials Technology Center, 114 Thailand Science Park, Pahonyothin Rd, Khlong Luang, Patumthani, 12120, Thailand.
authors.forename: Nootsara
authors.initials: N
authors.lastname: Narumol
authors.affiliation: Microbial Biotechnology and Biochemicals Research Unit, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Pahonyothin Rd, Khlong Luang, Patumthani, 12120, Thailand.
authors.forename: Verawat
authors.initials: V
authors.lastname: Champreda
authors.affiliation: Microbial Biotechnology and Biochemicals Research Unit, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Pahonyothin Rd, Khlong Luang, Patumthani, 12120, Thailand.
authors.forename: Lily
authors.initials: L
authors.lastname: Eurwilaichitr
authors.affiliation: Faculty of Biotechnology, Atmajaya Catholic University, Jl. Jend. Sudirman 51, Jakarta, 12930, Indonesia.
authors.forename: Antonius
authors.initials: A
authors.lastname: Suwanto
authors.affiliation: Microbial Biotechnology and Biochemicals Research Unit, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Pahonyothin Rd, Khlong Luang, Patumthani, 12120, Thailand. thidarat.nim@biotec.or.th.
authors.forename: Thidarat
authors.initials: T
authors.lastname: Nimchua
authors.affiliation: Microbial Biotechnology and Biochemicals Research Unit, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Pahonyothin Rd, Khlong Luang, Patumthani, 12120, Thailand. duriya@biotec.or.th.
authors.forename: Duriya
authors.initials: D
authors.lastname: Chantasingh
mesh_terms: D001230:Aspergillus / Q000201:enzymology* / Q000235:genetics; D061249:Batch Cell Culture Techniques / Q000379:methods*; D019149:Bioreactors / Q000382:microbiology; D032561:Cotton Fiber; D003902:Detergents / Q000737:chemistry / Q000378:metabolism; D008422:Materials Testing; D010843:Pichia / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D011096:Polygalacturonase / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013784:Textiles
chemical_list: D003902:Detergents; D011994:Recombinant Proteins; D011096:Polygalacturonase
keywords: Acidic pectinase; Bioscouring; Cotton fabric; Heterologous expression; High cell density fermentation