title: Macrophage migration inhibitory factor siRNA inhibits hepatic metastases of colorectal cancer cells.
abstract: The purpose of this study was to assess the anti-tumor effects of macrophage migration inhibitory factor (MIF) siRNA on colorectal cancer in a mouse xenograft model. MIF specific siRNA (MIF siRNA) or a nonspecific control siRNA was introduced to murine colorectal cancer CT-26 cells. Mouse xenograft models of colorectal cancer were established. MIF siRNA, control siRNA or water was injected twice a week intravenously for 4 weeks. MIF siRNA inhibited the proliferation and migration, while induced apoptosis of CT-26 cellsin vitro. Injection of MIF siRNA resulted in a significant decrease of serum MIF and VEGF levels, and the weight and volume of cecum-grafted tumorsin vivo. In contrast, the number of apoptotic cells and caspase-3 expression were increased by MIF siRNA in cecum graft tumor tissues. Moreover, the water and fodder consumption were significantly improved by MIF siRNA treatment. Importantly, MIF siRNA reduced the hepatic metastases from colorectal cancer. Our results suggest that siRNA targeting MIF is a promising agent for the treatment of hepatic metastasis of colorectal cancer cells.
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, Guangdong 510080, China.
authors.forename: Li-Hao
authors.initials: LH
authors.lastname: Wu
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, Guangdong 510080, China.
authors.forename: Harry Hua-Xiang
authors.initials: HH
authors.lastname: Xia
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, Guangdong 510080, China.
authors.forename: Wei-Qing
authors.initials: WQ
authors.lastname: Ma
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, Guangdong 510080, China.
authors.forename: Hao-Jie
authors.initials: HJ
authors.lastname: Zhong
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, Guangdong 510080, China.
authors.forename: Hui-Xian
authors.initials: HX
authors.lastname: Xu
authors.affiliation: Department of Gastroenterology, The 101 Hospital of the Chinese People\'s Liberation Army, Wuxi, Jiangsu 214000, China.
authors.forename: Ya-Min
authors.initials: YM
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Qingyuan Renmin Hospital, Qingyuan, Guangdong 511500, China.
authors.forename: Rong-Jiao
authors.initials: RJ
authors.lastname: Yang
authors.affiliation: Institute of Vascular Biology, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510080, China.
authors.forename: Li-Jing
authors.initials: LJ
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, Guangdong 510080, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, Guangdong 510080, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510080, Guangdong, China, 13724887928@qq.com.
authors.forename: Xing-Xiang
authors.initials: XX
authors.lastname: He
mesh_terms: D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics / Q000378:metabolism; D017209:Apoptosis; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D053181:Caspase 8 / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics / Q000473:pathology* / Q000628:therapy*; D015536:Down-Regulation; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000378:metabolism; D019746:Intramolecular Oxidoreductases / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000235:genetics / Q000517:prevention & control* / Q000556:secondary*; D008263:Macrophage Migration-Inhibitory Factors / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009361:Neoplasm Invasiveness; D034741:RNA, Small Interfering / Q000235:genetics; D000075684:T-Lymphoma Invasion and Metastasis-inducing Protein 1 / Q000235:genetics / Q000378:metabolism
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D000949:Histocompatibility Antigens Class II; D008263:Macrophage Migration-Inhibitory Factors; D034741:RNA, Small Interfering; D000075684:T-Lymphoma Invasion and Metastasis-inducing Protein 1; C513260:Tiam1 protein, mouse; C045084:invariant chain; C505409:Casp3 protein, mouse; C505526:Casp8 protein, mouse; D053148:Caspase 3; D053181:Caspase 8; D019746:Intramolecular Oxidoreductases; C076231:Mif protein, mouse