title: Polyphenol Interactions Mitigate the Immunogenicity and Allergenicity of Gliadins.
abstract: Wheat allergy is an IgE-mediated disorder. Polyphenols, which are known to interact with certain proteins, could be used to reduce allergic reactions. This study screened several polyphenol sources for their ability to interact with gliadins, mask epitopes, and affect basophil degranulation. Polyphenol extracts from artichoke leaves, cranberries, apples, and green tea leaves were examined. Of these extracts, the first three formed insoluble complexes with gliadins. Only the cranberry and apple extracts masked epitopes in dot blot assays using anti-gliadin IgG and IgE antibodies from patients with wheat allergies. The cranberry and artichoke extracts limited cellular degranulation by reducing mouse anti-gliadin IgE recognition. In conclusion, the cranberry extract is the most effective polyphenol source at reducing the immunogenicity and allergenicity of wheat gliadins.
authors.affiliation: UR 1268 Biopolymères Interactions Assemblages, INRA , F-44300 Nantes, France.|UP 2012.10.101 EGEAL Unit, Unilasalle , F-60000 Beauvais, France.|Guaranteed Gluten Free , F-80700 Roye, France.
authors.forename: Maxime
authors.initials: M
authors.lastname: Pérot
authors.affiliation: UR 1268 Biopolymères Interactions Assemblages, INRA , F-44300 Nantes, France.
authors.forename: Roberta
authors.initials: R
authors.lastname: Lupi
authors.affiliation: UR 1268 Biopolymères Interactions Assemblages, INRA , F-44300 Nantes, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Guyot
authors.affiliation: UP 2012.10.101 EGEAL Unit, Unilasalle , F-60000 Beauvais, France.
authors.forename: Carine
authors.initials: C
authors.lastname: Delayre-Orthez
authors.affiliation: UP 2012.10.101 EGEAL Unit, Unilasalle , F-60000 Beauvais, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Gadonna-Widehem
authors.affiliation: Guaranteed Gluten Free , F-80700 Roye, France.
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Thébaudin
authors.affiliation: UR 1268 Biopolymères Interactions Assemblages, INRA , F-44300 Nantes, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Bodinier
authors.affiliation: UR 1268 Biopolymères Interactions Assemblages, INRA , F-44300 Nantes, France.
authors.forename: Colette
authors.identifier: http://orcid.org/0000-0001-9585-9536
authors.initials: C
authors.lastname: Larré
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000276:immunology*; D000818:Animals; D001491:Basophils / Q000276:immunology; D005903:Gliadin / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry*; D011485:Protein Binding; D051381:Rats; D021182:Wheat Hypersensitivity / Q000276:immunology*
chemical_list: D000485:Allergens; D010936:Plant Extracts; D059808:Polyphenols; D007073:Immunoglobulin E; D005903:Gliadin
keywords: allergy; apple; artichoke leaf; basophils; cranberry; gliadins; green tea leaf; polyphenols; wheat