title: Prevalence and Clinical Impact of Human Pegivirus-1 Infection in HIV-1-Infected Individuals in Yunnan, China.
abstract: Human Pegivirus-1 (HPgV-1) may have a beneficial impact on disease progression in human immunodeficiency virus-1 (HIV-1) infection. However, analysis of the genotypic diversity of HPgV-1 and its relevance to the progression of HIV-1 disease remains limited. A total of 1062 HIV-1-infected individuals were recruited in all sixteen prefectures of Yunnan province, China. The reverse transcription nested polymerase chain reaction (RT-nPCR), phylogenetic analyses, and clinical data analyses were used to detect HPgV-1 infection, determine genotype, and analyze HPgV-1 genotype impact on HIV-1 disease progression. The overall positive rate of HPgV-1 RNA was 23.4% (248/1062), and the frequency of HPgV-1 infection in injecting drug users (IDUs) (28.5%, 131/460) was significantly higher than in heterosexuals (19.4%, 117/602). Multiple genotypes were identified in 212 subjects with successful sequencing for theE2gene, including genotype 7 (55.7%), genotype 3 (34.9%), genotype 4 (4.7%), genotype 2 (3.3%), and an unclassified group (1.4%). Moreover, genotype 7 predominated in IDUs, whereas genotype 3 was the most common in heterosexuals. Our results revealed that HPgV-1 genotype 7 groups exhibited significantly lower HIV-1 viral load and higher CD4⁺ cell counts. This finding suggests that HPgV-1 genotype 7 may be associated with a better progression of HIV-1 disease.
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China. miaozhijiang@yeah.net.
authors.forename: Zhijiang
authors.initials: Z
authors.lastname: Miao
authors.affiliation: Department of Infectious Diseases, Yunnan Provincial Hospital of Infectious Diseases, Kunming 650301, China. gaoli296@aliyun.com.
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China. yindisong@163.com.
authors.forename: Yindi
authors.initials: Y
authors.lastname: Song
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China. qjyangming@163.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Infectious Diseases, Yunnan Provincial Hospital of Infectious Diseases, Kunming 650301, China. zm050306@sohu.com.
authors.forename: Mi
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Infectious Diseases, Yunnan Provincial Hospital of Infectious Diseases, Kunming 650301, China. ljc666yn@163.com.
authors.forename: Jincheng
authors.initials: J
authors.lastname: Lou
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China. zy19860908@yeah.net.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Infectious Diseases, Yunnan Provincial Hospital of Infectious Diseases, Kunming 650301, China. wxch62597@foxmail.com.
authors.forename: Xicheng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China. fyky2005@163.com.
authors.forename: Yue
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Department of Infectious Diseases, Yunnan Provincial Hospital of Infectious Diseases, Kunming 650301, China. dongxq8001@126.com.
authors.forename: Xingqi
authors.initials: X
authors.lastname: Dong
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China. oliverxia2000@aliyun.com.
authors.forename: Xueshan
authors.initials: X
authors.lastname: Xia
mesh_terms: D000293:Adolescent; D000328:Adult; D002681:China / Q000453:epidemiology; D018450:Disease Progression; D016021:Epidemiologic Studies; D005260:Female; D018067:Flaviviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018178:Flaviviridae Infections / Q000453:epidemiology* / Q000473:pathology / Q000821:virology; D005838:Genotype; D015658:HIV Infections / Q000150:complications* / Q000473:pathology; D015497:HIV-1 / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015995:Prevalence; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019562:Viral Load; D055815:Young Adult
keywords: HCV; HIV-1; HPgV-1; clinical effect; co-infection; genotypic diversity; heterosexuals; injecting drug users