title: A novel subgenotype 3a hepatitis E virus isolated from pigs in China.
abstract: Hepatitis E virus (HEV) infection is an emerging disease with zoonotic transmission that represents a serious public health concern, especially in developing countries. Here we characterize a novel HEV strain CCST-517, which possesses a complete genome sequence of 7284 bp with typical HEV genome organization including 5' and 3' non-coding regions and three open reading frames. The sequence identities of CCST-517 with known HEV genotype 1, 2, 3, and 4 were 73.4-73.7, 73.2, 80.4-90.4, and 75.1-75.7%, respectively. Phylogenetic analysis clustered CCST-517 to the clade of HEV genotype 3a, together with the Japanese human HEV isolate (HE-JA10) and United States human HEV isolate (HEV-US2). Similarity plot analysis indicated that the fragment extending from 4500 to 5500 nt included evidence of one intra-genotype recombination event in the genome sequence of the CCST-517 strain. To our knowledge, this is the first report of HEV genotype 3a with its complete genome sequence revealed in China. Our findings revealed a close phylogenetic relationship of CCST-517 to human HEV-US2 and HE-JA10, implying cross-species transmission of HEV between pigs and humans.
authors.affiliation: College of Veterinary Medicine, Jilin University, 5333 Xian Road, Changchun, 130062, Jilin, China.|Changchun Institute of Biological Products Co. Ltd., 3456 Xian Road, Changchun, 130062, Jilin, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Cao
authors.affiliation: College of Veterinary Medicine, Jilin University, 5333 Xian Road, Changchun, 130062, Jilin, China.
authors.forename: Lisai
authors.initials: L
authors.lastname: Zhu
authors.affiliation: College of Veterinary Medicine, Jilin University, 5333 Xian Road, Changchun, 130062, Jilin, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Jilin University, 5333 Xian Road, Changchun, 130062, Jilin, China.
authors.forename: Changming
authors.initials: C
authors.lastname: Guo
authors.affiliation: College of Veterinary Medicine, Jilin University, 5333 Xian Road, Changchun, 130062, Jilin, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Changchun Institute of Biological Products Co. Ltd., 3456 Xian Road, Changchun, 130062, Jilin, China.
authors.forename: Chengbo
authors.initials: C
authors.lastname: Shi
authors.affiliation: Changchun Institute of Biological Products Co. Ltd., 3456 Xian Road, Changchun, 130062, Jilin, China. lzh63@163.com.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Jilin University, 5333 Xian Road, Changchun, 130062, Jilin, China. wangxp88@hotmail.com.|Key Laboratory for Zoonosis, Ministry of Education, Institute for Zoonosis of Jilin University, Changchun, 130062, China. wangxp88@hotmail.com.
authors.forename: Xinping
authors.initials: X
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002681:China; D016679:Genome, Viral; D005838:Genotype; D016751:Hepatitis E / Q000662:veterinary* / Q000821:virology; D016752:Hepatitis E virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D012367:RNA, Viral / Q000032:analysis; D011995:Recombination, Genetic; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D000073336:Whole Genome Sequencing; D015047:Zoonoses / Q000821:virology
chemical_list: D012367:RNA, Viral
keywords: Genetic recombination; HEV; Hepatitis E; Phylogenetic analysis; Zoonosis