title: Molecularly imprinted solid phase extraction method for simultaneous determination of seven nitroimidazoles from honey by HPLC-MS/MS.
abstract: In this work, a selective sample cleanup procedure that combined molecular imprinting technique with solid phase extraction was developed for the simultaneous extraction of the seven nitroimidazoles (NMZs) from honey samples. The molecular imprinting polymers for NMZs were prepared through bulk polymerization method using 2-methyl-5-nitroimidazole as template molecule, methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross linking agent. The obtained molecular imprinting polymers showed high affinity to template molecule and was used as selective sorbent for simultaneously selective extraction of the seven NMZs from honey matrix. An off-line molecularly imprinted solid phase extraction (MISPE) method followed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS) for simultaneous determination of the seven NMZs from honey samples was also established. The proposed method was validated at 1.0, 2.0 and 10.0μg/kg, obtaining recoveries in the range of 79.7-110%, with repeatability and interday precision values (expressed as relative standard deviation) ≤11.4% and ≤15.2%, respectively. Limits of quantification for different NMZs were 1.0μg/kg, which were always below the minimum required performance limits established by the European Community Reference Laboratories (Commission Decision 2002/657/EC). It was demonstrated that this proposed MISPE-HPLC-MS-MS method could be applied to direct determination of NMZs from honey samples.
authors.affiliation: Institute of Chinese Materia Medica, Pharmaceutical College of Henan University, Kaifeng 475004, China.
authors.forename: Xiu-Chun
authors.initials: XC
authors.lastname: Guo
authors.affiliation: Institute of Chinese Materia Medica, Pharmaceutical College of Henan University, Kaifeng 475004, China.
authors.forename: Zhao-Yang
authors.initials: ZY
authors.lastname: Xia
authors.affiliation: The Key Laboratory for Special Functional Materials of MOE, Henan University, Kaifeng 475004, China.
authors.forename: Hai-Hui
authors.initials: HH
authors.lastname: Wang
authors.affiliation: Institute of Chinese Materia Medica, Pharmaceutical College of Henan University, Kaifeng 475004, China.
authors.forename: Wen-Yi
authors.initials: WY
authors.lastname: Kang
authors.affiliation: Technology Center, Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, China.
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Lin
authors.affiliation: Technology Center, Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, China.
authors.forename: Wen-Qing
authors.initials: WQ
authors.lastname: Cao
authors.affiliation: Technology Center, Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, China.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Zhang
authors.affiliation: The Key Laboratory for Special Functional Materials of MOE, Henan University, Kaifeng 475004, China. Electronic address: zhouwh@henu.edu.cn.
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Zhou
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D006722:Honey / Q000032:analysis*; D054802:Molecular Imprinting; D009593:Nitroimidazoles / Q000032:analysis* / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry; D013997:Time Factors
chemical_list: D009593:Nitroimidazoles
keywords: Honey; Molecular imprinting polymers (MIPs); Nitroimidazoles; Simultaneous determination; Solid phase extraction (SPE)