title: Significance of Glypican-3 (GPC3) Expression in Hepatocellular Cancer Diagnosis.
abstract: BACKGROUND Primary hepatocellular carcinoma (HCC) is a malignant tumor that is common China. Early diagnosis is of great significance for improving treatment efficiency. GPC3 level is closely related to HCC occurrence. This study investigated GPC3 expression in HCC patient serum and tissue, and assessed the significance of GPC3 combined AFP detection in HCC diagnosis. MATERIAL AND METHODS A total of 76 HCC patients in our hospital were enrolled. Immunohistochemistry was applied to test GPC3 expression in cancer tissue and para-carcinoma tissue. ELISA and RT-PCR were used to detect GPC3 and AFP level in serum. The significance of GPC3 single or combined AFP detection in HCC diagnosis was analyzed. RESULTS Immunohistochemistry showed that the GPC3 positive expression rate was obviously elevated in HCC tissue (P<0.01). Combination detection of AFP and GPC3 presented significantly higher sensitivity and specificity in HCC than single AFP or GPC3 detection. ELISA showed no significant difference in sensitivity, specificity, or accuracy compared with RT-PCR. CONCLUSIONS Serum GPC3 was overexpressed in HCC patients. Combination detection of serum AFP and GPC3 can enhance accuracy and efficacy of HCC diagnosis.
authors.affiliation: Department of Interventional Radiology, The First People's Hospital of Guiyang, Guiyang, Guizhou, China (mainland).
authors.forename: Bin
authors.initials: B
authors.lastname: Sun
authors.affiliation: Department of Interventional Radiology, The Affiliated Baiyun Hospital of Guizhou Medical University, Guiyang, Guizhou, China (mainland).
authors.forename: Zhi
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Department of Prepotency, Maternal and Child Health Hospital of Guiyang City, Guiyang, Guizhou, China (mainland).
authors.forename: Bi
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou, China (mainland).
authors.forename: Yanglong
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Interventional Radiology, The First People's Hospital of Guiyang, Guiyang, Guizhou, China (mainland).
authors.forename: Shihai
authors.initials: S
authors.lastname: Lin
authors.affiliation: Department of Interventional Radiology, The First People's Hospital of Guiyang, Guiyang, Guizhou, China (mainland).
authors.forename: Lei
authors.initials: L
authors.lastname: Luo
authors.affiliation: Department of Interventional Radiology, The First People's Hospital of Guiyang, Guiyang, Guizhou, China (mainland).
authors.forename: Yuzhu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Interventional Radiology, The First People's Hospital of Guiyang, Guiyang, Guizhou, China (mainland).
authors.forename: Zheng
authors.initials: Z
authors.lastname: Huang
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology*; D005260:Female; D015870:Gene Expression; D053673:Glypicans / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000097:blood / Q000175:diagnosis / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D000509:alpha-Fetoproteins / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; C105937:GPC3 protein, human; D053673:Glypicans; D000509:alpha-Fetoproteins