title: Modulation of cholesterol transport by maternal hypercholesterolemia in human full-term placenta.
abstract: The significance of maternal cholesterol transporting to the fetus under normal as well as pathological circumstances is less understood. The objective of this study was to observe the effects of maternal hypercholesterolemia on placental cholesterol transportation. Human full-time placenta, maternal and venous cord blood were sampled at delivery from the pregnant women with serum total cholesterol (TC) concentrations at third trimester higher than 7.25 mM (n = 19) and the pregnant women with normal TC concentrations (n = 19). Serum lipids and expression of genes related to cholesterol transportation were measured by western blot or real-time PCR. The results indicated that serum TC, high density lipoprotein cholesterol (HDL-C), and low density lipoprotein cholesterol (LDL-C) levels were significantly increased, in pregnancies, but decreased in cord blood in hypercholesterolemic group compared to the matched control group. All the subjects were no-drinking, non-smoker, and gestational disease free. The mRNA expression of lipoprotein receptors, including LDLR and VLDLR were significantly increased, while the protein expression of PCSK9 was significantly increased in hypercholesterolemic placenta. In conclusion, maternal hypercholesterolemia might decrease the transportation of cholesterol from mother to fetus because of the high levels of PCSK9 protein expression.
authors.affiliation: School of Public Health, Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, People's Republic of China.
authors.forename: Ran
authors.initials: R
authors.lastname: Zhang
authors.affiliation: School of Public Health, Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, People's Republic of China.
authors.forename: Shan
authors.initials: S
authors.lastname: Dong
authors.affiliation: School of Public Health, Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, People's Republic of China.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Ma
authors.affiliation: School of Public Health, Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, People's Republic of China.
authors.forename: Xue-Ping
authors.initials: XP
authors.lastname: Cai
authors.affiliation: School of Public Health, Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, People's Republic of China.
authors.forename: Zhi-Yin
authors.initials: ZY
authors.lastname: Le
authors.affiliation: School of Public Health, Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, People's Republic of China.
authors.forename: Rong
authors.initials: R
authors.lastname: Xiao
authors.affiliation: Xuanwu hospital, Capital Medical University, Beijing, People's Republic of China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: School of Public Health, Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, People's Republic of China.
authors.forename: Huan-Ling
authors.initials: HL
authors.lastname: Yu
mesh_terms: D000070998:ATP Binding Cassette Transporter, Subfamily G, Member 1 / Q000235:genetics / Q000378:metabolism; D000328:Adult; D016022:Case-Control Studies; D002784:Cholesterol / Q000097:blood*; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D005260:Female; D005312:Fetal Blood / Q000378:metabolism; D006801:Humans; D006937:Hypercholesterolemia / Q000378:metabolism / Q000473:pathology*; D000071518:Liver X Receptors / Q000235:genetics / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011263:Pregnancy Trimester, Third; D000071449:Proprotein Convertase 9 / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D018110:Receptors, Lipoprotein / Q000235:genetics / Q000378:metabolism
chemical_list: C419353:ABCG1 protein, human; D000070998:ATP Binding Cassette Transporter, Subfamily G, Member 1; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D000071518:Liver X Receptors; D012333:RNA, Messenger; D018110:Receptors, Lipoprotein; D002784:Cholesterol; C472125:PCSK9 protein, human; D000071449:Proprotein Convertase 9