title: Aneuploidy screening by array comparative genomic hybridization improves success rates ofin vitrofertilization: A multicenter Indian study.
abstract: OBJECTIVE
  To evaluate the usefulness of preimplantation genetic screening (PGS) using array comparative genomic hybridization (aCGH) in the Indian population.
  
  
  METHODS
  This is a retrospective, multicenter study including 235 PGS cycles following intracytoplasmic sperm injection performed at six different infertility centers from September 2013 to June 2015. Patients were divided as per maternal age in several groups (<35, 35-36, 37-38, 39-40, and >40 years) and as per indication for undergoing PGS. Indications for performing PGS were recurrent miscarriage, repetitive implantation failure, severe male factor, previous trisomic pregnancy, and advanced maternal age (≥35). Day 3 embryo biopsy was performed and analyzed by aCGH followed by day 5 embryo transfer in the same cycle or the following cycle. Outcomes such as pregnancy rates (PRs)/transfer, implantation rates, miscarriage rates, percentage of abnormal embryos, and number of embryos with more than one aneuploidy and chaotic patterns were recorded for all the treated subjects based on different age and indication groups.
  
  
  RESULTS
  aCGH helped in identifying aneuploid embryos, thus leading to consistent implantation (range: 33.3%-42.9%) and PRs per transfer (range: 31.8%-54.9%) that were obtained for all the indications in all the age groups, after performing PGS.
  
  
  CONCLUSIONS
  Aneuploidy is one of the major factors which affect embryo implantation. aCGH can be successfully employed for screening of aneuploid embryos. When euploid embryos are transferred, an increase in PRs can be achieved irrespective of the age or the indication.
authors.affiliation: Nova IVI Fertility, Ahmedabad, Gujarat, India.
authors.forename: Aditi
authors.initials: A
authors.lastname: Kotdawala
authors.affiliation: Nova IVI Fertility, Ahmedabad, Gujarat, India.
authors.forename: Deven
authors.initials: D
authors.lastname: Patel
authors.affiliation: Nova IVI Fertility, Bengaluru, Karnataka, India.
authors.forename: Javier
authors.initials: J
authors.lastname: Herrero
authors.affiliation: Iviomics Research Pvt. Ltd., New Delhi, India.
authors.forename: Rajni
authors.initials: R
authors.lastname: Khajuria
authors.affiliation: Nova IVI Fertility, New Delhi, India.
authors.forename: Nalini
authors.initials: N
authors.lastname: Mahajan
authors.affiliation: Nova IVI Fertility, Ahmedabad, Gujarat, India.
authors.forename: Manish
authors.initials: M
authors.lastname: Banker
keywords: Aneuploidy; array comparative genomic hybridization; chromosome aberrations; preimplantation genetic screening