title: Systematic genetic dissection of PTS in Vibrio cholerae uncovers a novel glucose transporter and a limited role for PTS during infection of a mammalian host.
abstract: A common mechanism for high affinity carbohydrate uptake in microbial species is the phosphoenolpyruvate-dependent phosphotransferase system (PTS). This system consists of a shared component, EI, which is required for all PTS transport, and numerous carbohydrate uptake transporters. In Vibrio cholerae, there are 13 distinct PTS transporters. Due to genetic redundancy within this system, the carbohydrate specificity of each of these transporters is not currently defined. Here, using multiplex genome editing by natural transformation (MuGENT), we systematically dissect PTS transport in V. cholerae. Specifically, we generated a mutant strain that lacks all 13 PTS transporters, and from this strain, we created a panel of mutants where each expresses a single transporter. Using this panel, we have largely defined the carbohydrate specificities of each PTS transporter. In addition, this analysis uncovered a novel glucose transporter. We have further defined the mechanism of this transporter and characterized its regulation. Using our 13 PTS transporter mutant, we also provide the first clear evidence that carbohydrate transport by the PTS is not essential during infection in an infant mouse model of cholera. In summary, this study shows how multiplex genome editing can be used to rapidly dissect complex biological systems and genetic redundancy in microbial systems.
authors.affiliation: Department of Biology, Indiana University, Bloomington, IN, USA.
authors.forename: Chelsea A
authors.initials: CA
authors.lastname: Hayes
authors.affiliation: Department of Biology, Indiana University, Bloomington, IN, USA.
authors.forename: Triana N
authors.initials: TN
authors.lastname: Dalia
authors.affiliation: Department of Biology, Indiana University, Bloomington, IN, USA.
authors.forename: Ankur B
authors.identifier: 0000-0003-2203-1230
authors.initials: AB
authors.lastname: Dalia
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D001692:Biological Transport / Q000235:genetics; D002771:Cholera / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods; D004195:Disease Models, Animal; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D005947:Glucose / Q000378:metabolism; D051246:Glucose Transport Proteins, Facilitative / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics; D051379:Mice; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics* / Q000378:metabolism*; D014734:Vibrio cholerae / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D051246:Glucose Transport Proteins, Facilitative; D026901:Membrane Transport Proteins; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D005947:Glucose