title: Structure elucidation and immunological activity of a novel glycopeptide from mannatide.
abstract: A water-soluble glycopeptide, designated as MTW, was isolated and purified from crude mannatide by DEAE-Sepharose. MTW showed one symmetrical peak on HPGPC with an average molecular weight of 2.95×104Da. MTW contained 16 kinds of amino acids and the total amino acid content was 0.95%. The structure of polysaccharide moiety of MTW was elucidated based on monosaccharide composition, methylation analysis, partial acid hydrolysis, IR and 1D/2D NMR spectroscopy. The results showed that MTW was a homogeneous glycopeptide including mannose and glucose with a molar ratio of 2:1. The polysaccharide moiety of MTW had a backbone of (1→6)-α-d-Man p residues, which highly branched at O-2 position of (1→2,6)-α-d-Man p residues. The side chains were mainly composed of (1→)-α-d-Man p, (1→2)-α-d-Man p, (1→4)-α-d-Glc p, (1→4,6)-α-d-Glc p residues. The backbone of the polysaccharide moiety of MTW was the same as MT2-A (Li et al. [1]), which was the main component of mannatide, but the side chains of MTW were somewhat different from that of MT2-A. Complement-fixation test indicated that MTW had the same beneficial effect on immunological activity as MT2-A.
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China.
authors.forename: Mengqi
authors.initials: M
authors.lastname: Wu
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China.
authors.forename: Zhizhen
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Chengdu Leer Pharmaceutical Co. Ltd, Chengdu 610083, PR China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Wan
authors.affiliation: Chengdu Leer Pharmaceutical Co. Ltd, Chengdu 610083, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China.
authors.forename: Wenqing
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China. Electronic address: xiawei1999@ecust.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Xia
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D006020:Glycopeptides / Q000737:chemistry* / Q000276:immunology*; D006868:Hydrolysis; D008745:Methylation; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D012756:Sheep
chemical_list: D006020:Glycopeptides; D009005:Monosaccharides; C064233:polyactin A
keywords: Immunological activity; Mannatide; Polysaccharide; Structure