title: Identification and Characterization of Lipopolysaccharide Induced TNFα Factor from Blunt Snout Bream, Megalobrama amblycephala.
abstract: Lipopolysaccharide induced TNFα factor (LITAF) is an important transcription factor responsible for regulation of tumor necrosis factor α. In this study, a novellitafgene (designated asMalitaf) was identified and characterized from blunt snout bream,Megalobrama amblycephala.The full-length cDNA ofMalitafwas of 956 bp, encoding a polypeptide of 161 amino acids with high similarity to other known LITAFs. A phylogenetic tree also showed thatMalitafsignificantly clustered with those of other teleost, indicating thatMalitafwas a new member of fish LITAF family. The putative maLITAF protein possessed a highly conserved LITAF domain with two CXXC motifs. The mRNA transcripts ofMalitafwere detected in all examined tissues of healthyM.amblycephala, including kidney, head kidney, muscle, liver, spleen, gill, and heart, and with the highest expression in immune organs: spleen and head kidney. The expression level ofMalitafin spleen was rapidly up-regulated and peaked (1.29-fold,p< 0.05) at 2 h after lipopolysaccharide (LPS) stimulation. Followed the stimulation ofMalitaf,Matnfαtranscriptional level was also transiently induced to a high level (51.74-fold,p< 0.001) at 4 h after LPS stimulation. Taken together, we have identified a putative fish LITAF ortholog, which was a constitutive and inducible immune response gene involved inM.amblycephalainnate immunity during the course of a pathogenic infection.
authors.affiliation: Department of Cell Biology, School of Life Sciences, Central South University, Changsha 410017, China. lvyina@csu.edu.cn.
authors.forename: Yina
authors.initials: Y
authors.lastname: Lv
authors.affiliation: Center of Biological Experiments, School of Life Sciences, Central South University, Changsha 410017, China. bestxxy@126.com.
authors.forename: Xinying
authors.initials: X
authors.lastname: Xiang
authors.affiliation: Department of Cell Biology, School of Life Sciences, Central South University, Changsha 410017, China. jiangyuhong@csu.edu.cn.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Cell Biology, School of Life Sciences, Central South University, Changsha 410017, China. tangleilei142511021@163.com.
authors.forename: Leilei
authors.initials: L
authors.lastname: Tang
authors.affiliation: Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning 530021, China. zhouyi1982cn@126.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning 530021, China. zhonghuanzh@126.com.
authors.forename: Huan
authors.initials: H
authors.lastname: Zhong
authors.affiliation: Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning 530021, China. dreamshaw@hotmail.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Department of Cell Biology, School of Life Sciences, Central South University, Changsha 410017, China. andy_yan@csu.edu.cn.
authors.forename: Jinpeng
authors.initials: J
authors.lastname: Yan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005399:Fishes / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D015870:Gene Expression; D007113:Immunity, Innate; D008070:Lipopolysaccharides / Q000276:immunology; D009928:Organ Specificity / Q000235:genetics; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D014157:Transcription Factors
keywords: Megalobrama amblycephala; innate immune; lipopolysaccharide induced TNFα factor; lipopolysaccharide stimulation