title: An integrated microfluidic device for rapid and high-sensitivity analysis of circulating tumor cells.
abstract: Recently there has been a more focus on the development of an efficient technique for detection of circulating tumor cells (CTCs), due to their significance in prognosis and therapy of metastatic cancer. However, it remains a challenge because of the low count of CTCs in the blood. Herein, a rapid and high-sensitivity approach for CTCs detection using an integrated microfluidic system, consisting of a deterministic lateral displacement (DLD) isolating structure, an automatic purifying device with CD45-labeled immunomagnetic beads and a capturing platform coated with rat-tail collagen was reported. We observed high capture rate of 90%, purity of about 50% and viability of more than 90% at the high throughput of 1 mL/min by capturing green fluorescent protein (GFP)-positive cells from blood. Further capturing of CTCs from metastatic cancers patients revealed a positive capture rate of 83.3%. Furthermore, our device was compared with CellSearch system via parallel analysis of 30 cancer patients, to find no significant difference between the capture efficiency of both methods. However, our device displayed advantage in terms of time, sample volume and cost for analysis. Thus, our integrated device with sterile environment and convenient use will be a promising platform for CTCs detection with potential clinical application.
authors.affiliation: Department of Respiratory Medicine, The Second Hospital Affiliated to Dalian Medical University, Dalian 116027, China.
authors.forename: Jianing
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of Respiratory Medicine, The Second Hospital Affiliated to Dalian Medical University, Dalian 116027, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
authors.forename: Weiliang
authors.initials: W
authors.lastname: Shu
authors.affiliation: Department of Respiratory Medicine, The Second Hospital Affiliated to Dalian Medical University, Dalian 116027, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Tian
authors.affiliation: LABVIV Technology(Shenzhen) Co., Ltd, Shenzhen 518055, China.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Huang
authors.affiliation: College of Marine Engineering, Dalian Maritime University, Dalian 116026, China.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Respiratory Medicine, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, China.
authors.forename: Ruoyu
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Respiratory Medicine, The Second Hospital Affiliated to Dalian Medical University, Dalian 116027, China.
authors.forename: Encheng
authors.initials: E
authors.lastname: Li
authors.affiliation: Department of Medicine, Division of Hematology Oncology, Medical School of University of California at Los Angeles, Los Angeles 90095, CA, USA.
authors.forename: Dennis
authors.initials: D
authors.lastname: Slamon
authors.affiliation: Department of Medicine, Division of Hematology Oncology, Medical School of University of California at Los Angeles, Los Angeles 90095, CA, USA.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Hou
authors.affiliation: Department of Respiratory Medicine, The Second Hospital Affiliated to Dalian Medical University, Dalian 116027, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Du
authors.affiliation: Department of Respiratory Medicine, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, China.
authors.forename: Lichuan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Respiratory Medicine, The Second Hospital Affiliated to Dalian Medical University, Dalian 116027, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D002470:Cell Survival; D003131:Combined Modality Therapy; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008297:Male; D046210:Microfluidic Analytical Techniques; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D009367:Neoplasm Staging; D009369:Neoplasms / Q000175:diagnosis* / Q000628:therapy; D009360:Neoplastic Cells, Circulating / Q000473:pathology*
chemical_list: D015415:Biomarkers