title: Peptidomics Analysis of Transient Regeneration in the Neonatal Mouse Heart.
abstract: Neonatal mouse hearts have completely regenerative capability after birth, but the ability to regenerate rapidly lost after 7 days, the mechanism has not been clarified. Previous studies have shown that mRNA profile of adult mouse changed greatly compared to neonatal mouse. So far, there is no research of peptidomics related to heart regeneration. In order to explore the changes of proteins, enzymes, and peptides related to the transient regeneration, we used comparative petidomics technique to compare the endogenous peptides in the mouse heart of postnatal 1 and 7 days. In final, we identified 236 differentially expressed peptides, 169 of which were upregulated and 67 were downregulated in the postnatal 1 day heart, and also predicted 36 functional peptides associated with transient regeneration. The predicted 36 candidate peptides are located in the important domains of precursor proteins and/or contain the post-transcriptional modification (PTM) sites, which are involved in the biological processes of cardiac development, cardiac muscle disease, cell proliferation, necrosis, and apoptosis. In conclusion, for the first time, we compared the peptidomics profiles of neonatal heart between postnatal 1 day and postnatal 7 day. This study provides a new direction and an important basis for the mechanism research of transient regeneration in neonatal heart. J. Cell. Biochem. 118: 2828-2840, 2017. © 2017 Wiley Periodicals, Inc.
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Qijun
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Zijie
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Departments of Cardiology, Taizhou People's Hospital, Taizhou 225300, China.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Liansheng
authors.initials: L
authors.lastname: Wang
authors.affiliation: Departments of Cardiology, Taizhou People's Hospital, Taizhou 225300, China.
authors.forename: Guixian
authors.initials: G
authors.lastname: Song
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Lingmei
authors.initials: L
authors.lastname: Qian
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D006321:Heart / Q000502:physiology*; D051379:Mice; D009124:Muscle Proteins / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000502:physiology*; D012038:Regeneration / Q000502:physiology*
chemical_list: D009124:Muscle Proteins
keywords: HEART REGENERATION; MASS SPECTROMETRY; NEONATAL MOUSE; PEPTIDOMICS