title: FRET study in oligopeptide-linked donor-acceptor system in PVA matrix.
abstract: An oligopeptide: Lys-Gly-Pro-Arg-Ser-Leu-Ser-Gly-Lys-NH2, cleaved specifically by a matrix metalloproteinase 9 (MMP-9) at the Ser-Leu bond, was labeled on the ε-NH2groups of lysine with donor (5, 6 TAMRA) and acceptor (HiLyte647) dye. The donor control was a peptide labeled with 5, 6 TAMRA only on the C-terminal lysine, and the acceptor control was free HiLyte647. Following three products were studied by dissolving in 10% (w/w) poly(vinyl alcohol) and dried on glass slides forming 200 micron films. Absorption spectra of the films show full additivity of donor and acceptor absorptions. A strong Fluorescence Resonance Energy Transfer (FRET) with an efficiency of about 85% was observed in the fluorescence emission and excitation spectra. The lifetime of the donor was shorter and heterogeneous compared with the donor control.
authors.affiliation: Institute of Molecular Medicine, Center for Fluorescence Technologies and Nanomedicine, University of North Texas Health Science Center, Fort Worth, TX 76107, USA.
authors.forename: Sunil
authors.initials: S
authors.lastname: Shah
authors.forename: Wlodek
authors.initials: W
authors.lastname: Mandecki
authors.forename: Ji
authors.initials: J
authors.lastname: Li
authors.forename: Zygmunt
authors.initials: Z
authors.lastname: Gryczynski
authors.forename: Julian
authors.initials: J
authors.lastname: Borejdo
authors.forename: Ignacy
authors.initials: I
authors.lastname: Gryczynski
authors.forename: Rafal
authors.initials: R
authors.lastname: Fudala
mesh_terms: D000595:Amino Acid Sequence; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes; D020778:Matrix Metalloproteinase 2; D020783:Matrix Metalloproteinase 7; D020780:Matrix Metalloproteinase 9; D009842:Oligopeptides; D010446:Peptide Fragments; D010455:Peptides
chemical_list: D005456:Fluorescent Dyes; D009842:Oligopeptides; D010446:Peptide Fragments; D010455:Peptides; D020783:Matrix Metalloproteinase 7; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9