title: Reliability of glutamate and GABA quantification using proton magnetic resonance spectroscopy.
abstract: The consistency and reliability of proton magnetic resonance spectroscopy (1H-MRS) assessments of neurotransmitter concentration has not been widely examined over multiple days. The purpose of this study was to determine the reliability of glutamate and GABA measures using a single-voxel1H-MRS protocol in healthy men and women. Glutamate and GABA quantitations were obtained from the primary motor cortex (M1) and the dorsolateral prefrontal cortex (DLPFC) in 13 healthy individuals across 3 data collection sessions, including a baseline (Visit 1), 2-week (Visit 2), and 2-month time point (Visit 3). Glutamate concentrations were similar across visits in M1 (p=0.72) and the DLPFC (p=0.52). Reliability across days was excellent in M1 (R=0.93), and in the DLPFC (R=0.99). GABA concentrations were similar across visits in M1 (p=0.44) and in the DLPFC (p=0.59). Reliability of GABA concentration across days was excellent in M1 (R=0.93), and in the DLPFC (R=0.97).1H-MRS is a reliable method for quantifying glutamate and GABA concentration in M1 and the DLPFC in humans.
authors.affiliation: Department of Human Physiology, University of Oregon, Eugene, OR, 97403, United States.
authors.forename: Alia L
authors.initials: AL
authors.lastname: Yasen
authors.affiliation: Robert and Beverly Lewis Center for NeuroImaging, University of Oregon, OR, 97403, United States.
authors.forename: Jolinda
authors.initials: J
authors.lastname: Smith
authors.affiliation: Department of Human Physiology, University of Oregon, Eugene, OR, 97403, United States. Electronic address: adc1@uoregon.edu.
authors.forename: Anita D
authors.initials: AD
authors.lastname: Christie
mesh_terms: D000328:Adult; D001921:Brain / Q000378:metabolism*; D005260:Female; D018698:Glutamic Acid / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D018377:Neurotransmitter Agents / Q000378:metabolism*; D066244:Proton Magnetic Resonance Spectroscopy / Q000379:methods; D015203:Reproducibility of Results; D055815:Young Adult; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D018377:Neurotransmitter Agents; D005973:Glutamine; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid
keywords: GABA; Glutamate; Magnetic resonance spectroscopy; Reliability