title: Assaying Low-Density-Lipoprotein (LDL) Uptake into Cells.
abstract: Determination of LDL particle uptake into cells is a valuable technique in the field of cholesterol metabolism. This allows assessment of LDL uptake capacity in different adherent and non-adherent cells types, as well as the effect of cellular, genetic, or pharmacological perturbations on this process. Here, we detail a general procedure that describes the production of fluorescently-labeled LDL particles and quantitative and non-quantitative assays for determining cellular LDL uptake.
authors.affiliation: Department of Medical Biochemistry, Academic Medical Center, University of Amsterdam, Meibergdreef 15, Amsterdam, 1105AZ, The Netherlands. a.loregger@amc.uva.nl.
authors.forename: Anke
authors.initials: A
authors.lastname: Loregger
authors.affiliation: Department of Medical Biochemistry, Academic Medical Center, University of Amsterdam, Meibergdreef 15, Amsterdam, 1105AZ, The Netherlands.
authors.forename: Jessica K
authors.initials: JK
authors.lastname: Nelson
authors.affiliation: Department of Medical Biochemistry, Academic Medical Center, University of Amsterdam, Meibergdreef 15, Amsterdam, 1105AZ, The Netherlands. n.zelcer@amc.uva.nl.
authors.forename: Noam
authors.initials: N
authors.lastname: Zelcer
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D056945:Hep G2 Cells; D006801:Humans; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000494:pharmacology; D013194:Staining and Labeling
chemical_list: D005456:Fluorescent Dyes; D008077:Lipoproteins, LDL
keywords: Cholesterol metabolism; Endocytosis; LDL; LDLR