title: Ultrasensitive Ebola Virus Detection Based on Electroluminescent Nanospheres and Immunomagnetic Separation.
abstract: The 2014-16 Ebola virus (EBOV) outbreak in West Africa has attracted widespread concern. Rapid and sensitive detection methods are urgently needed for diagnosis and treatment of the disease. Here, we propose a novel method for EBOV detection based on efficient amplification of electroluminescent nanospheres (ENs) coupled with immunomagnetic separation. Uniform ENs are made by embedding abundant amounts of CdSe/ZnS quantum dots (QDs) into copolymer nanospheres through simple ultrasound. Compared to QDs, ENs can enhance electroluminescence (ECL) signals by approximately 85-fold, achieving a signal-to-background ratio high enough for EBOV detection. The introduction of magnetic nanobeads (MBs) can selectively separate targets from complex samples, simplifying the operation process and saving time. The presence of MBs can amplify ECL by approximately 3-fold, improving detection sensitivity. By integration of ENs with MBs, a sensitive electroluminescence biosensor is established for EBOV detection. The linear range is 0.02-30 ng/mL with a detection limit of 5.2 pg/mL. This method provides consistent reproducibility, specificity, and anti-interference ability and is highly promising in clinical diagnosis applications.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, Wuhan University , Wuhan 430072, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, Wuhan University , Wuhan 430072, People's Republic of China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Hu
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, Wuhan University , Wuhan 430072, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, Wuhan University , Wuhan 430072, People's Republic of China.
authors.forename: Zhi-Ling
authors.identifier: 0000-0001-7807-2264
authors.initials: ZL
authors.lastname: Zhang
authors.affiliation: Center for Influenza Research and Early warning (CASCIRE), Chinese Academy of Sciences , Beijing 100101, People's Republic of China.|CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences , Hubei 430071, People's Republic of China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Chen
authors.affiliation: Center for Influenza Research and Early warning (CASCIRE), Chinese Academy of Sciences , Beijing 100101, People's Republic of China.|CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101, People's Republic of China.
authors.forename: Gary
authors.initials: G
authors.lastname: Wong
authors.affiliation: Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, Department of Medical Microbiology, University of Manitoba , Winnipeg, Manitoba R3E 3R2, Canada.
authors.forename: Xiangguo
authors.initials: X
authors.lastname: Qiu
authors.affiliation: Center for Influenza Research and Early warning (CASCIRE), Chinese Academy of Sciences , Beijing 100101, People's Republic of China.|CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101, People's Republic of China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Liu
authors.affiliation: Center for Influenza Research and Early warning (CASCIRE), Chinese Academy of Sciences , Beijing 100101, People's Republic of China.|CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101, People's Republic of China.|Shenzhen Key Laboratory of Pathogen and Immunity, State Key Discipline of Infectious Disease, Shenzhen Third People's Hospital , Shenzhen 518112, People's Republic of China.
authors.forename: George F
authors.initials: GF
authors.lastname: Gao
authors.affiliation: Center for Influenza Research and Early warning (CASCIRE), Chinese Academy of Sciences , Beijing 100101, People's Republic of China.|CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101, People's Republic of China.|Shenzhen Key Laboratory of Pathogen and Immunity, State Key Discipline of Infectious Disease, Shenzhen Third People's Hospital , Shenzhen 518112, People's Republic of China.
authors.forename: Yuhai
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, Wuhan University , Wuhan 430072, People's Republic of China.
authors.forename: Dai-Wen
authors.initials: DW
authors.lastname: Pang
mesh_terms: D015374:Biosensing Techniques; D029043:Ebolavirus / Q000302:isolation & purification*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D005240:Feasibility Studies; D006046:Gold / Q000737:chemistry; D019142:Hemorrhagic Fever, Ebola / Q000175:diagnosis; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D057230:Limit of Detection; D049449:Luminescence; D046529:Microscopy, Electron, Transmission; D054118:Nanospheres; D045663:Quantum Dots; D015203:Reproducibility of Results
chemical_list: D006046:Gold