title: Morroniside protects SK-N-SH human neuroblastoma cells against H2O2-induced damage.
abstract: Oxidative stress-induced cell injury has been linked to the pathogenesis of neurodegenerative disorders such as spinal cord injury, Parkinson's disease, and multiple sclerosis. Morroniside is an antioxidant derived from the Chinese herb Shan-Zhu-Yu. The present study investigated the neuroprotective effect of morroniside against hydrogen peroxide (H2O2)-induced cell death in SK-N-SH human neuroblastoma cells. H2O2 increased cell apoptosis, as determined by flow cytometry and Hoechst 33342 staining. This effect was reversed by pretreatment with morroniside at concentrations of 1-100 µM. The increase in intracellular reactive oxygen species (ROS) generation and lipid peroxidation induced by H2O2 was also abrogated by morroniside. H2O2 induced a reduction in mitochondrial membrane potential, increased caspase-3 activity, and caused downregulation of B cell lymphoma-2 (Bcl-2) and upregulation of Bcl-2-associated X protein (Bax) expression. These effects were blocked by morroniside pretreatment. Thus, morroniside protects human neuroblastoma cells against oxidative damage by inhibiting ROS production while suppressing Bax and stimulating Bcl-2 expression, thereby blocking mitochondrial-mediated apoptosis. These results indicate that morroniside has therapeutic potential for the prevention and treatment of neurodegenerative diseases.
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, P.R. China.
authors.forename: Jing-Xing
authors.initials: JX
authors.lastname: Zhang
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, Bengbu, Anhui 233004, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, Bengbu, Anhui 233004, P.R. China.
authors.forename: Jin
authors.initials: J
authors.lastname: Xi
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, Bengbu, Anhui 233004, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Shen
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, P.R. China.
authors.forename: An-You
authors.initials: AY
authors.lastname: Zhu
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, Bengbu, Anhui 233004, P.R. China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Qi
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, Bengbu, Anhui 233004, P.R. China.
authors.forename: Qi-Yi
authors.initials: QY
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, P.R. China.
authors.forename: Lun-Jun
authors.initials: LJ
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, P.R. China.
authors.forename: Feng-Chao
authors.initials: FC
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, P.R. China.
authors.forename: He-Zuo
authors.initials: HZ
authors.lastname: Lü
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, P.R. China.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Hu
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006027:Glycosides / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D020782:Matrix Metalloproteinases / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D009447:Neuroblastoma / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D016877:Oxidants / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; D006027:Glycosides; D018696:Neuroprotective Agents; D016877:Oxidants; D017382:Reactive Oxygen Species; C488401:morroniside; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D053148:Caspase 3; D020782:Matrix Metalloproteinases