title: Comparison of dietary inclusion of commercial and fermented soybean meal on oxidative status and non-specific immune responses in white shrimp, Litopenaeus vannamei.
abstract: This study compared the effects of partial replacement of fish meal (FM) protein with commercial soybean meal (SBM) or Lactobacillus spp. fermented SBM (FSBM) on the oxidative status and non-specific immune responses in white shrimps, Litopenaeus vannamei. A FM-based diet was used as the control. To prepare the two experimental diets, 25% of the FM protein was replaced with SBM and FSBM. Three experimental diets were fed to three groups of shrimps (initial wt: 0.63 ± 0.01 g) in a recirculating rearing system for 12 weeks. The SBM-diet group had the highest hepatopancreatic thiobarbituric acid-reactive substance value, followed by the FSBM-diet group, and the lowest in control-diet group. The activity of hepatopancreatic superoxide dismutase was highest in the control-diet group, followed by the FSBM-diet group, and was lowest in the SBM-diet group. The total haemocyte, hyaline cell, semigranular cell, and granular cell counts were highest in the control-diet group, followed by the FSBM-diet group and the SBM-fed group. Haemolymph phenoloxidase activity was higher in the control-diet and FSBM-diet groups than in the SBM-diet group. The results indicate that replacing 25% FM protein with SBM significantly reduces non-specific immune responses and induces oxidative stress in white shrimps and that FSBM prepared using Lactobacillus spp. can reduce these negative effects.
authors.affiliation: Department of Aquaculture, National Pingtung University of Science and Technology, 1 Shuefu Road, Neipu, Pingtung 912, Taiwan, ROC. Electronic address: yuhunglin@mail.npust.edu.tw.
authors.forename: Yu-Hung
authors.initials: YH
authors.lastname: Lin
authors.affiliation: Department of Aquaculture, National Pingtung University of Science and Technology, 1 Shuefu Road, Neipu, Pingtung 912, Taiwan, ROC.
authors.forename: Jia-Jinn
authors.initials: JJ
authors.lastname: Mui
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D004032:Diet; D005285:Fermentation; D007113:Immunity, Innate / Q000502:physiology; D018384:Oxidative Stress / Q000502:physiology; D033561:Penaeidae / Q000276:immunology*; D013025:Glycine max / Q000737:chemistry*
keywords: Fermentation; Immune responses; Oxidative status; Soybean meal; White shrimp