title: 125I-F56 Peptide as Radioanalysis Agent Targeting VEGFR1 in Mice Xenografted with Human Gastric Tumor.
abstract: 125I-Radiolabeled F56 peptide was designed as a radioactive analogue of F56 (peptide WHSDMEWWYLLG) to bind with VEGFR1 receptor. It was synthesized in high radiochemical yield and specific activity. Thein vitrostability of125I-F56 was tested, and the bioactivity of125I-F56 was confirmed by both cell uptake and binding affinity measurement in VEGFR1 positive BGC-823 cells. The time-radioactivity relationship and biodistribution of125I-F56 tracer were conducted using nude mice bearing human gastric carcinoma BGC-823, by noninvasive micro-SPECT/CT imaging. The tracer's tumor uptake was further confirmed by autoradiography and HE stain of125I-F56 in tumor tissues ex vivo. Those results demonstrated that125I-F56 holds great potential as a diagnostic agent in both molecular imaging and radioanalysis probe for gastric cancer.
authors.affiliation: Department of Nuclear Medicine and Department of Biochemistry and Molecular Biology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute , Beijing 100142, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Nuclear Medicine and Department of Biochemistry and Molecular Biology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute , Beijing 100142, China.
authors.forename: Chuanke
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Department of Nuclear Medicine and Department of Biochemistry and Molecular Biology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute , Beijing 100142, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Nuclear Medicine and Department of Biochemistry and Molecular Biology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute , Beijing 100142, China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Nuclear Medicine and Department of Biochemistry and Molecular Biology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute , Beijing 100142, China.
authors.forename: Junnan
authors.initials: J
authors.lastname: Feng
authors.affiliation: Department of Nuclear Medicine and Department of Biochemistry and Molecular Biology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute , Beijing 100142, China.
authors.forename: Chengchao
authors.initials: C
authors.lastname: Shou
authors.affiliation: Department of Nuclear Medicine and Department of Biochemistry and Molecular Biology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute , Beijing 100142, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Yang
keywords: 125I−F56; gastric cancer; radioanalysis; vascular endothelial growth factor receptor 1