title: Self-Assembled Nanoconstructs Modified with Amplified Aptamers Inhibited Tumor Growth and Retinal Vascular Hyperpermeability via Vascular Endothelial Growth Factor Capturing.
abstract: Here, nanoconstructs consisting of a DNA-amplified aptamer with a biocompatible polymer backbone for capturing target biomolecules are presented. First, the polymer-DNA nanoconstructs were prepared by hybridization of two complementary single-stranded DNAs that were each conjugated to a dextran polymer backbone. The designed polymer-DNA amplified aptamer nanoconstructs (PA-aNCs) were then prepared by utilizing polymer-DNA nanoconstructs conjugated with an aptamer (PA-NCs) using a rolling circle amplification reaction to amplify the aptamer. These PA-aNCs were successfully applied to alleviate tumor growth and vascular endothelial growth factor (VEGF)-induced retinal vascular hyperpermeability in vivo through the highly effective capture of human VEGF as a target molecule. These PA-aNCs could be used as therapeutic agent for anti-VEGF therapy by efficiently capturing human VEGF.
authors.affiliation: Center for Self-Assembly and Complexity, Institute for Basic Science (IBS) , Pohang 37673, Republic of Korea.|Department of Chemistry, Pohang University of Science and Technology (POSTECH) , Pohang 37673, Republic of Korea.
authors.forename: Jihyun
authors.initials: J
authors.lastname: Lee
authors.affiliation: Fight Against Angiogenesis-Related Blindness Laboratory, Clinical Research Institute, Seoul National University Hospital , Seoul 03080, Republic of Korea.|Department of Biomedical Sciences, College of Medicine, Seoul National University , Seoul 03080, Republic of Korea.
authors.forename: Byung Joo
authors.initials: BJ
authors.lastname: Lee
authors.affiliation: Center for Self-Assembly and Complexity, Institute for Basic Science (IBS) , Pohang 37673, Republic of Korea.|Department of Chemistry, Pohang University of Science and Technology (POSTECH) , Pohang 37673, Republic of Korea.
authors.forename: Yeong Mi
authors.initials: YM
authors.lastname: Lee
authors.affiliation: School of Integrative Engineering, Chung-Ang University , Seoul 156-751, Republic of Korea.
authors.forename: Hansoo
authors.initials: H
authors.lastname: Park
authors.affiliation: Fight Against Angiogenesis-Related Blindness Laboratory, Clinical Research Institute, Seoul National University Hospital , Seoul 03080, Republic of Korea.|Department of Biomedical Sciences, College of Medicine, Seoul National University , Seoul 03080, Republic of Korea.|Department of Ophthalmology, College of Medicine, Seoul National University , Seoul 03080, Republic of Korea.
authors.forename: Jeong Hun
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Center for Self-Assembly and Complexity, Institute for Basic Science (IBS) , Pohang 37673, Republic of Korea.|Department of Chemistry, Pohang University of Science and Technology (POSTECH) , Pohang 37673, Republic of Korea.
authors.forename: Won Jong
authors.identifier: 0000-0002-4064-0999
authors.initials: WJ
authors.lastname: Kim
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D007150:Immunohistochemistry; D049329:Nanostructures / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D042461:Vascular Endothelial Growth Factor A / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D011108:Polymers; D042461:Vascular Endothelial Growth Factor A; D004247:DNA
keywords: DNA nanoconstructs; anti-VEGF therapy; antitumor therapy; polymer−DNA conjugates; retinal vascular hyperpermeability