title: Surfactant protein A (SP-A) and SP-A-derived peptide attenuate chemotaxis of mast cells induced by human β-defensin 3.
abstract: Human β-defensin 3 (hBD3) is known to be involved in mast cell activation. However, molecular mechanisms underlying the regulation of hBD3-induced mast cell activation have been poorly understood. We previously reported that SP-A and SP-A-derived peptide 01 (SAP01) regulate the function of hBD3. In this study, we focused on the effects of SP-A and SAP01 on the activation of mast cells induced by hBD3. SAP01 directly bound to hBD3. Mast cell-mediated vascular permeability and edema in hBD3 administered rat ears were decreased when injected with SP-A or SAP01. Compatible with the results in rat ear model, both SP-A and SAP01 inhibited hBD3-induced chemotaxis of mast cells in vitro. Direct interaction between SP-A or SAP01 and hBD3 seemed to be responsible for the inhibitory effects on chemotaxis. Furthermore, SAP01 attenuated hBD3-induced accumulation of mast cells and eosinophils in tracheas of the OVA-sensitized inflammatory model. SP-A might contribute to the regulation of inflammatory responses mediated by mast cells during infection.
authors.affiliation: Department of Biochemistry, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, Japan; Department of Respiratory Medicine and Allergology, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, Japan.
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Uehara
authors.affiliation: Department of Biochemistry, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, Japan.
authors.forename: Motoko
authors.initials: M
authors.lastname: Takahashi
authors.affiliation: Department of Pathology, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Murata
authors.affiliation: Department of Biochemistry, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, Japan; Department of Respiratory Medicine and Allergology, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Saito
authors.affiliation: Department of Biochemistry, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, Japan.
authors.forename: Rina
authors.initials: R
authors.lastname: Takamiya
authors.affiliation: Department of Biochemistry, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, Japan; Department of Respiratory Medicine and Allergology, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Hasegawa
authors.affiliation: Department of Respiratory Medicine and Allergology, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Kuronuma
authors.affiliation: Department of Respiratory Medicine and Allergology, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Chiba
authors.affiliation: Department of Biochemistry, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, Japan; Department of Urologic Surgery and Andrology, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, Japan.
authors.forename: Jiro
authors.initials: J
authors.lastname: Hashimoto
authors.affiliation: Department of Pathology, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, Japan.
authors.forename: Norimasa
authors.initials: N
authors.lastname: Sawada
authors.affiliation: Department of Respiratory Medicine and Allergology, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: Department of Biochemistry, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kuroki
authors.affiliation: Department of Biochemistry, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, Japan; Department of Chemistry, Sapporo Medical University Center for Medical Education, S-1 W-17, Chuo-ku, Sapporo, Japan. Electronic address: sarikscb@sapmed.ac.jp.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Ariki
mesh_terms: D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects; D002633:Chemotaxis / Q000187:drug effects*; D004487:Edema / Q000188:drug therapy / Q000276:immunology; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000276:immunology*; D008297:Male; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology; D037662:Pulmonary Surfactant-Associated Protein A / Q000737:chemistry / Q000276:immunology* / Q000494:pharmacology; D017207:Rats, Sprague-Dawley; D023083:beta-Defensins / Q000276:immunology*
chemical_list: C422950:DEFB103A protein, human; D010455:Peptides; D037662:Pulmonary Surfactant-Associated Protein A; D023083:beta-Defensins
keywords: Chemotaxis; Human β-defensin 3; Mast cells; Surfactant protein A