title: [Effect of high frequency electrotherapy on caspase-3 and ultra microstructure of hippocampus in rats following cerebral ischemia/reperfusion].
abstract: To investigate the effect of high frequency electrotherapy (HFE) on rat hippocampus after cerebral ischemia/reperfusion (I/R).  Methods: A rat model of cerebral I/R injury was established. The rats were randomly divided into a sham group, an I/R group and an HFE group. The HFE group received thearapy daily for different sessions for 1, 3, 7 d. Neuronal deficit score,neuron ultra microstructure in the hippocampus and caspase-3 protein expression were measured on 1 st, 3 th and 7th d.  Results: Compared with the I/R group, the HFE group showed the decreased neurological deficit scores, with significant differences between the 2 groups (P<0.05). The injury in HFE group was reduced compared with that in the I/R group based on the electron microscope test, with significant difference. Caspase-3 protein in brain tissue in the HFE group also downregulated compared with that in the I/R group (P<0.05).  Conclusion: High frequency electrotherapy can improve neural function, suppress caspase-3 expression and apoptosis in nerve cells and improve the ultra microstructure of neurons, displaying a protective effect on cerebral I/R injury in rats.
authors.affiliation: Department of Rehabilitation, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Department of Rehabilitation, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Rumi
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Rehabilitation, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Changjie
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Rehabilitation, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Wenna
authors.initials: W
authors.lastname: Peng
authors.affiliation: Department of Rehabilitation, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yin
authors.affiliation: Department of Neurology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Hu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D001923:Brain Chemistry / Q000528:radiation effects; D002545:Brain Ischemia / Q000503:physiopathology / Q000532:radiotherapy; D053148:Caspase 3 / Q000378:metabolism* / Q000528:radiation effects*; D002544:Cerebral Infarction / Q000503:physiopathology* / Q000532:radiotherapy*; D004599:Electric Stimulation Therapy; D006624:Hippocampus / Q000737:chemistry / Q000503:physiopathology / Q000528:radiation effects; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000503:physiopathology / Q000532:radiotherapy
chemical_list: D053148:Caspase 3