title: High-fat meal, systemic inflammation and glucose homeostasis in obese children and adolescents.
abstract: We aimed to assess in obese youths the relationships between interleukin-6 (IL-6), fat meal-induced endotoxemia and glucose homeostasis. Twenty obese children/adolescents (9-17 years old, 11 boys) underwent a standard oral glucose tolerance test and, 7-14 days later, a 5-h fat meal test (fat=69% of energy, saturated/monounsaturated/polyunsaturated fatty acids=31.5%/35%/33.5%), with serial measures of IL-6 and two markers of lipopolysaccharide (LPS) exposure and translocation, LPS-binding protein (LBP) and soluble CD14 (sCD14). IL-6 correlated not only with basal (homeostatic model assessment-insulin resistance) but also with post-prandial (Matsuda index) insulin sensitivity (r=0.61 (0.24-0.82), P=0.005, r=-0.53 (0.12-0.78), P=0.03, respectively). IL-6 did not change after the meal whereas LBP and sCD14 decreased significantly, indicating LPS translocation. Neither basal sCD14 and LBP nor their incremental concentrations correlated with IL-6 or glucose homeostasis. In our sample, IL-6 was associated with insulin sensitivity but not with LPS exposure, suggesting that meals with a balanced content of saturated/monounsaturated/polyunsaturated fatty acids may not be associated with LPS-induced inflammation and metabolic impairment.
authors.affiliation: Pediatric Diabetes and Metabolic Disorders Unit, Department of Surgery, Dentistry, Paediatrics and Gynaecology, University of Verona, Verona, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Morandi
authors.affiliation: Pediatric Diabetes and Metabolic Disorders Unit, Department of Surgery, Dentistry, Paediatrics and Gynaecology, University of Verona, Verona, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Fornari
authors.affiliation: Pediatric Diabetes and Metabolic Disorders Unit, Department of Surgery, Dentistry, Paediatrics and Gynaecology, University of Verona, Verona, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Opri
authors.affiliation: Pediatric Diabetes and Metabolic Disorders Unit, Department of Surgery, Dentistry, Paediatrics and Gynaecology, University of Verona, Verona, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Corradi
authors.affiliation: Pediatric Diabetes and Metabolic Disorders Unit, Department of Surgery, Dentistry, Paediatrics and Gynaecology, University of Verona, Verona, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Tommasi
authors.affiliation: Division of Endocrinology, Department of Clinical and Experimental Medicine, University Hospital of Parma, Parma, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Bonadonna
authors.affiliation: Pediatric Diabetes and Metabolic Disorders Unit, Department of Surgery, Dentistry, Paediatrics and Gynaecology, University of Verona, Verona, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Maffeis
mesh_terms: D000293:Adolescent; D001786:Blood Glucose / Q000378:metabolism*; D015992:Body Mass Index; D002648:Child; D059305:Diet, High-Fat / Q000009:adverse effects*; D019446:Endotoxemia / Q000097:blood* / Q000209:etiology; D005260:Female; D005951:Glucose Tolerance Test; D006706:Homeostasis; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism; D015850:Interleukin-6 / Q000097:blood; D007558:Italy; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology; D008297:Male; D062407:Meals; D063766:Pediatric Obesity / Q000097:blood / Q000150:complications / Q000378:metabolism*; D019518:Postprandial Period / Q000502:physiology
chemical_list: D001786:Blood Glucose; C508600:IL6 protein, human; D007328:Insulin; D015850:Interleukin-6; D008070:Lipopolysaccharides