title: Label-free Detection of Influenza Viruses using a Reduced Graphene Oxide-based Electrochemical Immunosensor Integrated with a Microfluidic Platform.
abstract: Reduced graphene oxide (RGO) has recently gained considerable attention for use in electrochemical biosensing applications due to its outstanding conducting properties and large surface area. This report presents a novel microfluidic chip integrated with an RGO-based electrochemical immunosensor for label-free detection of an influenza virus, H1N1. Three microelectrodes were fabricated on a glass substrate using the photolithographic technique, and the working electrode was functionalized using RGO and monoclonal antibodies specific to the virus. These chips were integrated with polydimethylsiloxane microchannels. Structural and morphological characterizations were performed using X-ray photoelectron spectroscopy and scanning electron microscopy. Electrochemical studies revealed good selectivity and an enhanced detection limit of 0.5 PFU mL-1, where the chronoamperometric current increased linearly with H1N1 virus concentration within the range of 1 to 104PFU mL-1(R2= 0.99). This microfluidic immunosensor can provide a promising platform for effective detection of biomolecules using minute samples.
authors.affiliation: School of Mechanical and Nuclear Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea.
authors.forename: Renu
authors.initials: R
authors.lastname: Singh
authors.affiliation: School of Mechanical and Nuclear Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea.
authors.forename: Seongkyeol
authors.initials: S
authors.lastname: Hong
authors.affiliation: School of Mechanical and Nuclear Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea.|Department of Biomedical Engineering, UNIST, Ulsan 44919, Republic of Korea.
authors.forename: Jaesung
authors.initials: J
authors.lastname: Jang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology / Q000302:isolation & purification*; D057230:Limit of Detection; D008839:Microelectrodes; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*
chemical_list: D006108:Graphite