title: Stimulated Raman scattering microscopy and spectroscopy with a rapid scanning optical delay line.
abstract: Stimulated Raman scattering (SRS) microscopy that is capable of both high-speed imaging and rapid spectroscopy will be advantageous for detailed chemical analysis of heterogeneous biological specimens. We have developed a system based on spectral focusing SRS technology with the integration of a rapid scanning optical delay line, which allows continuous tuning of SRS spectra by scanning a galvo mirror. We demonstrate SRS spectral measurements of dimethyl sulfoxide solution at low concentrations and multi-color imaging of rice pollens and HeLa cells with line-by-line delay tuning to reduce motion artifacts, as well as fast acquisition of SRS spectra at specific regions of interest.
authors.forename: Ruoyu
authors.initials: R
authors.lastname: He
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Liu
authors.forename: Yongkui
authors.initials: Y
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Hong
authors.initials: H
authors.lastname: Ma
authors.forename: Minbiao
authors.initials: M
authors.lastname: Ji
mesh_terms: D006367:HeLa Cells; D006801:Humans; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D055096:Optical Devices; D012275:Oryza; D011058:Pollen / Q000166:cytology; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors