title: Somatic molecular subtyping of prostate tumors from HOXB13 G84E carriers.
abstract: A recurrent germline mutation (G84E) in the HOXB13 gene is associated with early onset and family history-positive prostate cancer in patients of European descent, occurring in up to 5% of prostate cancer families. To date, the molecular features of prostate tumors occurring in HOXB13 G84E carriers have not been studied in a large cohort of patients. We identified 101 heterozygous carriers of G84E who underwent radical prostatectomy for prostate cancer between 1985 and 2011 and matched these men by race, age and tumor grade to 99 HOXB13 wild-type controls. Immunostaining for HOXB13, PTEN, ERG, p53 and SPINK1 as well as RNA in situ hybridization for ETV1/4/5 were performed using genetically validated assays. Tumors from G84E carriers generally expressed HOXB13 protein at a level comparable to benign and wild-type glands. ETS gene expression (either ERG or ETV1/4/5) was seen in 36% (36/101) of tumors from G84E carriers compared to 68% (65/96) of the controls (p < 0.0001). PTEN was lost in 11% (11/101) of G84E carriers compared to 25% (25/99) of the controls (p = 0.014). PTEN loss was enriched among ERG-positive compared to ERG-negative tumors in both groups of patients. Nuclear accumulation of the p53 protein, indicative of underlying TP53 missense mutations, was uncommon in both groups, occurring in 1% (1/101) of the G84E carriers versus 2% (2/92) of the controls (p = NS). Taken together, these data suggest that genes other than ERG and PTEN may drive carcinogenesis/progression in the majority of men with germline HOXB13 mutations.
authors.affiliation: Departments of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.|Departments of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Tamara L
authors.initials: TL
authors.lastname: Lotan
authors.affiliation: Departments of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Alba
authors.initials: A
authors.lastname: Torres
authors.affiliation: Departments of Pathology, MD Anderson Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Miao
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Departments of Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: Tosoian
authors.affiliation: Departments of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Liana B
authors.initials: LB
authors.lastname: Guedes
authors.affiliation: Departments of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Helen
authors.initials: H
authors.lastname: Fedor
authors.affiliation: Departments of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Jessica
authors.initials: J
authors.lastname: Hicks
authors.affiliation: Departments of Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Charles M
authors.initials: CM
authors.lastname: Ewing
authors.affiliation: Departments of Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Sarah D
authors.initials: SD
authors.lastname: Isaacs
authors.affiliation: Departments of Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Dorhyun
authors.initials: D
authors.lastname: Johng
authors.affiliation: Departments of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.|Departments of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.|Departments of Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Angelo M
authors.initials: AM
authors.lastname: De Marzo
authors.affiliation: Departments of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.|Departments of Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: William B
authors.initials: WB
authors.lastname: Isaacs
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D016022:Case-Control Studies; D005500:Follow-Up Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D018095:Germ-Line Mutation / Q000235:genetics*; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis; D011471:Prostatic Neoplasms / Q000145:classification / Q000235:genetics* / Q000473:pathology; D012307:Risk Factors
chemical_list: D014408:Biomarkers, Tumor; C484414:HOXB13 protein, human; D018398:Homeodomain Proteins
keywords: ERG; HOXB13 G84E; PTEN; familial prostate cancer; prostatic adenocarcinoma