title: Interrogation of metabolic and oxygen states of tumors with fiber-based luminescence lifetime spectroscopy.
abstract: The study of metabolic and oxygen states of cells in a tumor in vivo is crucial for understanding of the mechanisms responsible for tumor development and provides background for the relevant tumor's treatment. Here, we show that a specially designed implantable fiber-optic probe provides a promising tool for optical interrogation of metabolic and oxygen states of a tumor in vivo. In our experiments, the excitation light from a ps diode laser source is delivered to the sample through an exchangeable tip via a multimode fiber, and the emission light is transferred to the detector by another multimode fiber. Fluorescence lifetime of a nicotinamid adenine dinucleotide (NAD(P)H) and phosphorescence lifetime of an oxygen sensor based on an iridium (III) complex of enzothienylpyridine (BTPDM1) are explored both in model experiment in solutions and in living mice.
authors.forename: Maria
authors.initials: M
authors.lastname: Lukina
authors.forename: Anna
authors.initials: A
authors.lastname: Orlova
authors.forename: Marina
authors.initials: M
authors.lastname: Shirmanova
authors.forename: Daniil
authors.initials: D
authors.lastname: Shirokov
authors.forename: Anton
authors.initials: A
authors.lastname: Pavlikov
authors.forename: Antje
authors.initials: A
authors.lastname: Neubauer
authors.forename: Hauke
authors.initials: H
authors.lastname: Studier
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Becker
authors.forename: Elena
authors.initials: E
authors.lastname: Zagaynova
authors.forename: Toshitada
authors.initials: T
authors.lastname: Yoshihara
authors.forename: Seiji
authors.initials: S
authors.lastname: Tobita
authors.forename: Vladislav
authors.initials: V
authors.lastname: Shcheslavskiy
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D006801:Humans; D007495:Iridium / Q000737:chemistry; D051379:Mice; D009249:NADP / Q000378:metabolism*; D055100:Optical Fibers; D009942:Organometallic Compounds / Q000737:chemistry; D010100:Oxygen / Q000378:metabolism*; D011725:Pyridines / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
chemical_list: D009942:Organometallic Compounds; D011725:Pyridines; D007495:Iridium; D009249:NADP; D010100:Oxygen