title: Intact wound repair activity of human mesenchymal stem cells after YM155 mediated selective ablation of undifferentiated human embryonic stem cells.
abstract: BACKGROUND
  Risk of teratoma formation during human pluripotent stem cell (hPSC)-based cell therapy is one of the technical hurdles that must be resolved before their wider clinical application. To this end, selective ablation of undifferentiated hPSCs has been achieved using small molecules whose application should be safe for differentiated cells derived from the hPSCs.
  
  
  OBJECTIVE
  However, the functional safety of such small molecules in the cells differentiated from hPSCs has not yet been extensively validated.
  
  
  METHODS
  We used the survivin inhibitor YM155, which induced highly selective cell death of hPSCs for ablating undifferentiated hESCs after differentiation to human mesenchymal stem cells (hMSCs) and examined whether hMSCs remained fully functional after being exposed by YM155.
  
  
  RESULTS
  We demonstrated that human mesenchymal stem cells (hMSCs) derived from human embryonic stem cells (hESCs) remained fully functional in vitro and in vivo, while hESCs were selectively ablated.
  
  
  CONCLUSIONS
  These results suggest that a single treatment with YM155 after differentiation of hMSCs would be a valid approach for teratoma-free cell therapy.
authors.affiliation: Department of Life Sciences, College of Natural Sciences, Sogang University, Seoul 121-742, Republic of Korea.
authors.forename: Keun-Tae
authors.initials: KT
authors.lastname: Kim
authors.affiliation: Department of Life Sciences, College of Natural Sciences, Sogang University, Seoul 121-742, Republic of Korea.
authors.forename: Ho-Chang
authors.initials: HC
authors.lastname: Jeong
authors.affiliation: Department of Medicine, School of Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: C-Yoon
authors.initials: CY
authors.lastname: Kim
authors.affiliation: Stem cell Research Center, Jeju National University, Jeju, Jeju Special Self-Governing Province, Republic of Korea; Mirae Cell Bio Co. LTD, Seoul, Republic of Korea.
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Kim
authors.affiliation: Department of Medicine, School of Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Si-Hyun
authors.initials: SH
authors.lastname: Heo
authors.affiliation: Department of Life Sciences, College of Natural Sciences, Sogang University, Seoul 121-742, Republic of Korea.
authors.forename: Seung-Ju
authors.initials: SJ
authors.lastname: Cho
authors.affiliation: Department of Medicine, School of Medicine, Konkuk University, Seoul 143-701, Republic of Korea. Electronic address: stemness@gmail.com.
authors.forename: Ki-Sung
authors.initials: KS
authors.lastname: Hong
authors.affiliation: Department of Life Sciences, College of Natural Sciences, Sogang University, Seoul 121-742, Republic of Korea. Electronic address: hjcha@sogang.ac.kr.
authors.forename: Hyuk-Jin
authors.initials: HJ
authors.lastname: Cha
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D016207:Cytokines / Q000378:metabolism; D000066449:Human Embryonic Stem Cells / Q000166:cytology*; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology*; D007150:Immunohistochemistry; D051034:Inhibitor of Apoptosis Proteins / Q000037:antagonists & inhibitors*; D007834:Lasers; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D009285:Naphthoquinones / Q000494:pharmacology*; D039904:Pluripotent Stem Cells / Q000166:cytology*; D000077022:Survivin; D014945:Wound Healing
chemical_list: C107489:BIRC5 protein, human; D003470:Culture Media; D016207:Cytokines; D007093:Imidazoles; D051034:Inhibitor of Apoptosis Proteins; D009285:Naphthoquinones; D000077022:Survivin; C523798:sepantronium
keywords: Embryonic stem cells; Mesenchymal stem cells; Teratoma; Wound repair; YM155