title: Evaluation of Rv0220, Rv2958c, Rv2994 and Rv3347c of Mycobacterium tuberculosis for serodiagnosis of tuberculosis.
abstract: Tuberculosis (TB), the leading cause of death among infectious diseases worldwide, is caused by Mycobacterium tuberculosis (M. tuberculosis). Early accurate diagnosis means earlier prevention, treatment and control of TB. To confirm efficient diagnostic antigens for M. tuberculosis, the serodiagnosis value of four recombinant proteins including Rv0220, Rv2958c, Rv2994 and Rv3347c was evaluated in this study. The specificities and sensitivities of four recombinant proteins were determined based on enzyme-linked immunosorbent assay (ELISA) by screening sera from smear-positive pulmonary TB patients (n = 92), uninfected individuals (n = 60) and patients with Mycoplasma pneumoniae (n = 32) that potentially cross-react with M. tuberculosis. The ELISAs showed that Rv0220, Rv2958c, Rv2994 and Rv3347c exhibited high specificities and sensitivities in detecting immunoglobulin G (IgG) antibody, with 98.3/91.3%, 91.7/85.9%, 93.3/89.1% and 93.3/80.4% respectively. According to the receiver-operating characteristic (ROC) analysis, the area under the ROC of the target proteins was 0.988, 0.969, 0.929 and 0.945 respectively. Western blot was established to evaluate the immunoreactivities of target proteins to mice and human sera. Results demonstrated that Rv0220, Rv2958c, Rv2994 and Rv3347c could specifically recognize TB-positive sera and the sera of mice immunized with the corresponding protein. Thus, Rv0220, Rv2958c, Rv2994 and Rv3347c were valuable potential diagnostic antigens for M. tuberculosis.
authors.affiliation: Institute of Pathogenic Biology, Medical College, University of South China, Hengyang, 421001, China.|Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang, 421001, China.|Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang, 421001, China.|Clinical laboratory, The First Affiliated Hospital of University of South China, Hengyang, 421000, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: You
authors.affiliation: Institute of Pathogenic Biology, Medical College, University of South China, Hengyang, 421001, China.|Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang, 421001, China.|Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang, 421001, China.
authors.forename: Ranhui
authors.initials: R
authors.lastname: Li
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control/National Institute for communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.
authors.forename: Kanglin
authors.initials: K
authors.lastname: Wan
authors.affiliation: Institute of Pathogenic Biology, Medical College, University of South China, Hengyang, 421001, China.|Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang, 421001, China.|Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang, 421001, China.
authors.forename: Liangzhuan
authors.initials: L
authors.lastname: Liu
authors.affiliation: Clinical laboratory, Hengyang No.1 People's Hospital, Hengyang, 421001, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Xie
authors.affiliation: Institute of Pathogenic Biology, Medical College, University of South China, Hengyang, 421001, China.|Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang, 421001, China.|Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang, 421001, China.
authors.forename: Lanhua
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Clinical laboratory, The First Affiliated Hospital of University of South China, Hengyang, 421000, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wu
authors.affiliation: Institute of Pathogenic Biology, Medical College, University of South China, Hengyang, 421001, China.|Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang, 421001, China.|Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang, 421001, China.
authors.forename: Xiangying
authors.initials: X
authors.lastname: Deng
authors.affiliation: Institute of Pathogenic Biology, Medical College, University of South China, Hengyang, 421001, China.|Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang, 421001, China.|Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang, 421001, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Institute of Pathogenic Biology, Medical College, University of South China, Hengyang, 421001, China.|Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang, 421001, China.|Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang, 421001, China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000276:immunology*; D012372:ROC Curve; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods*; D014376:Tuberculosis / Q000175:diagnosis*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D011994:Recombinant Proteins