title: A medically relevant capsular polysaccharide in Acinetobacter baumannii is a potential vaccine candidate.
abstract: Concerns of Acinetobacter baumannii infection have increased due to the emergence of multi-drug resistance. In the present study, we determined the capsular polysaccharide (CPS) structure of A. baumannii SK44, a clinical isolate from Taiwan, to consist of pentasaccharide repeats. We found that CPS-induced antibody provided 55% protection against challenge in an animal model. The CPS-specific antibody reacted with the surface components of about 62% clinical isolates (342/554 strains) from cross-sectional and longitudinal studies by dot-immunoassay. Pulsed-field gel electrophoresis of positive strains showed the antibody covered different clonalites of A. baumannii clinical isolates. Meanwhile, using the CPS antibody as a probe, we found a number of outer membrane proteins bound to the antibody, including OmpA/motB, TonB-dependent receptor, and Omp38, indicating their association with CPS. These results might lead to the use of the capsular polysaccharide as a vaccine to prevent A. baumannii infection.
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan.
authors.forename: Feng-Ling
authors.initials: FL
authors.lastname: Yang
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan.
authors.forename: Tze-Chi
authors.initials: TC
authors.lastname: Lou
authors.affiliation: National Health Research Institutes, Miaoli County 35053, Taiwan.
authors.forename: Shu-Chen
authors.initials: SC
authors.lastname: Kuo
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan.
authors.forename: Wan-Ling
authors.initials: WL
authors.lastname: Wu
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan.
authors.forename: Jeffy
authors.initials: J
authors.lastname: Chern
authors.affiliation: School of Medicine, National Yang-Ming University, Taipei 112, Taiwan; Department of Emergency Medicine, Taipei Veterans General Hospital, Taipei 112, Taiwan.
authors.forename: Yi-Tzu
authors.initials: YT
authors.lastname: Lee
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan.
authors.forename: Shui-Tsung
authors.initials: ST
authors.lastname: Chen
authors.affiliation: Human Health Therapeutics, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario K1A0R, Canada.
authors.forename: Wei
authors.initials: W
authors.lastname: Zou
authors.affiliation: Institute of Microbiology, Tzu Chi University, Hualien 907, Taiwan.
authors.forename: Nien-Tsung
authors.initials: NT
authors.lastname: Lin
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan. Electronic address: shwu@gate.sinica.edu.tw.
authors.forename: Shih-Hsiung
authors.initials: SH
authors.lastname: Wu
mesh_terms: D000151:Acinetobacter Infections / Q000382:microbiology / Q000517:prevention & control*; D040981:Acinetobacter baumannii / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D001428:Bacterial Vaccines / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D003430:Cross-Sectional Studies; D004195:Disease Models, Animal; D006801:Humans; D008137:Longitudinal Studies; D051379:Mice; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D013624:Taiwan
chemical_list: D001428:Bacterial Vaccines; D011135:Polysaccharides, Bacterial
keywords: Acinetobacter baumannii; Capsular polysaccharide; Clinic population distribution; Passive immunity