title: Promotion of cell growth and adhesion of a peptide hydrogel scaffold via mTOR/cadherin signaling.
abstract: Understanding neurite outgrowth, orientation, and migration is important for the design of biomaterials that interface with the neural tissue. However, the molecular signaling alternations have not been well elucidated to explain the impact of hydrogels on cell morphology. In our previous studies, a silk fibroin peptide (SF16) hydrogel was found to be an effective matrix for the viability, morphology, and proliferation of PC12 rat pheocrhomocytoma cells. We found that PC12 cells in the peptide hydrogel exhibited adhesive morphology compared to those cultured in agarose or collagen. Moreover, we identified that cell adhesion molecules (E- and N-cadherin) controlled by mTOR signaling were highly induced in PC12 cells cultured in the SF16 peptide hydrogel. Our findings suggest that the SF16 peptide might be suitable to be a cell-adhesion material in cell culture or tissue engineering, and mTOR/cadherin signaling is required for the cell adhesion in the SF16-peptide hydrogel.
authors.affiliation: Department of Orthopaedics, The 1st Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Guojun
authors.initials: G
authors.lastname: Wei
authors.affiliation: School of Pharmacy and Medical Sciences, Sansom Institute for Health Research, University of South Australia, Adelaide, South Austrslia, Australia.
authors.forename: Liping
authors.identifier: http://orcid.org/0000-0001-9355-1167
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, The 1st Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Daming
authors.initials: D
authors.lastname: Dong
authors.affiliation: Department of Orthopedics, The People's Hospital of Yuxi City, The 6th Affiliated Hospital of Kunming Medical University, Yuxi, Yunan, China.
authors.forename: Zhaowei
authors.initials: Z
authors.lastname: Teng
authors.affiliation: Department of Orthopaedics, The 1st Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Zuowei
authors.initials: Z
authors.lastname: Shi
authors.affiliation: Department of Orthopaedics, The 1st Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Kaifu
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, The 1st Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Gang
authors.initials: G
authors.lastname: An
authors.affiliation: Department of Orthopaedics, The 1st Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Guan
authors.affiliation: Department of Orthopaedics, The 1st Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Han
authors.affiliation: Department of Orthopaedics, The 2nd Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Yao
authors.affiliation: School of Pharmacy and Medical Sciences, Sansom Institute for Health Research, University of South Australia, Adelaide, South Austrslia, Australia.
authors.forename: Cory J
authors.initials: CJ
authors.lastname: Xian
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape; D005349:Fibroins / Q000378:metabolism*; D020100:Hydrogels; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000201:enzymology*; D016716:PC12 Cells; D010455:Peptides / Q000378:metabolism*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D051381:Rats; D015398:Signal Transduction; D020123:Sirolimus / Q000494:pharmacology; D058570:TOR Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D054457:Tissue Scaffolds
chemical_list: C000610228:CDH1 protein, rat; D015820:Cadherins; D020100:Hydrogels; C570099:N-cadherin, rat; D009419:Nerve Tissue Proteins; D010455:Peptides; D047428:Protein Kinase Inhibitors; C482288:fibroin, silkworm; D005349:Fibroins; C546845:mTOR protein, rat; D058570:TOR Serine-Threonine Kinases; D020123:Sirolimus
keywords: PC12 cells; SF16 peptide; cadherin; hydrogel; mTOR