title: Erysipelothrix rhusiopathiae recruits host plasminogen via the major protective antigen SpaA.
abstract: Erysipelothrix rhusiopathiae is the causative agent of animal erysipelas and human erysipeloid. Some pathogenic bacteria are able to recruit host plasminogen and then use the plasminogen system for migration across tissue barriers or for nutritional demands during infection. However, there is no study on E. rhusiopathiae recruitment of plasminogen. SpaA has long been known to be a major protective antigen of E. rhusiopathiae, but its roles in virulence have not yet been well clarified. The aim of this study was to detect the activity of E. rhusiopathiae to recruit host plasminogen and evaluate the ability of SpaA to act as a receptor in the recruitment process. It was found that E. rhusiopathiae could recruit host plasminogen. SpaA could specifically bind host plasminogen. Anti-SpaA serum could significantly decrease the activity of E. rhusiopathiae to recruit plasminogen. In addition, this binding activity was lysine dependent. In conclusion, E. rhusiopathiae was able to recruit host plasminogen via SpaA. To our knowledge, this is the first report on E. rhusiopathiae recruitment of host plasminogen and the receptor in the process.
authors.affiliation: Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.|College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.|College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.|College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Chengzhi
authors.initials: C
authors.lastname: Cai
authors.affiliation: Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.|College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Jingtao
authors.initials: J
authors.lastname: Li
authors.affiliation: Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.|College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wu
authors.affiliation: Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.|College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Kang
authors.affiliation: Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.|College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.|Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan 430070, China.|Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China.
authors.forename: Meilin
authors.initials: M
authors.lastname: Jin
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D004887:Erysipeloid / Q000382:microbiology; D004888:Erysipelothrix / Q000378:metabolism* / Q000472:pathogenicity; D054884:Host-Pathogen Interactions; D006801:Humans; D010958:Plasminogen / Q000378:metabolism*; D014774:Virulence
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C114457:SpaA protein, Erysipelothrix rhusiopathiae; D010958:Plasminogen
keywords: Erysipelothrix rhusiopathiae; SpaA; erysipelas; erysipeloid; plasminogen