title: Complete genome sequence of Lysinibacillus sphaericus LMG 22257, a strain with ureolytic activity inducing calcium carbonate precipitation.
abstract: Microbiologically induced calcium carbonate precipitation shows the potential for use in bioremediation and construction consolidation, but the efficiency of this process must be improved. Lysinibacillus sphaericus LMG 22257 is a gram-positive ureolytic strain that has recently been applied for consolidating construction by mediating calcium carbonate precipitation. The complete genome sequence of L. sphaericus LMG 22257 is 3,436,578 base pairs with a GC content of 38.99%. The urea degradation pathway and genes related to extracellular polymeric substance biosynthesis were also identified. The strain can tolerate high alkalinity (pH up to 10) and high urea concentration (up to 3M). These findings provide insights into the microbiologically induced carbonate precipitation and extend the application of the metabolic potential of L. sphaericus LMG 22257 for bioremediation.
authors.affiliation: State Key Laboratory of Microbial Metabolism, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Wenkai
authors.initials: W
authors.lastname: Yan
authors.affiliation: Institute of Oceanology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Ocean Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: zhang.yusjtu@sjtu.edu.cn.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D001406:Bacillaceae / Q000235:genetics*; D001482:Base Composition; D002119:Calcium Carbonate / Q000737:chemistry; D002874:Chromosome Mapping; D016680:Genome, Bacterial; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000379:methods*; D014508:Urea / Q000378:metabolism; D014510:Urease / Q000235:genetics
chemical_list: D014508:Urea; D014510:Urease; D002119:Calcium Carbonate
keywords: Biomineralization; Calcium carbonate; Extracellular polymeric substance; Lysinibacillus sphaericus LMG 22257; Urease