title: Multivalent foldamer-based affinity assay for selective recognition of Aβ oligomers.
abstract: Mimicking the molecular recognition functionality of antibodies is a great challenge. Foldamers are attractive candidates because of their relatively small size and designable interaction surface. This paper describes a sandwich type enzyme-linked immunoassay with a tetravalent β-peptide foldamer helix array as capture element and enzyme labeled tracer antibodies. The assay was found to be selective to β-amyloid oligomeric species with surface features transiently present in ongoing aggregation. In optimized conditions, with special emphasis on the foldamer immobilization, a detection limit of 5 pM was achieved with a linear range of 10-500 pM. These results suggest that protein mimetic foldamers can be useful tools in biosensors and affinity assays.
authors.affiliation: Institute of Pharmaceutical Analysis, University of Szeged, Somogyi u. 4, 6720 Szeged, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Olajos
authors.affiliation: Institute of Pharmaceutical Analysis, University of Szeged, Somogyi u. 4, 6720 Szeged, Hungary.
authors.forename: Éva
authors.initials: É
authors.lastname: Bartus
authors.affiliation: Department of Medical Chemistry, University of Szeged, Dóm tér 8, 6720 Szeged, Hungary.
authors.forename: Ildikó
authors.initials: I
authors.lastname: Schuster
authors.affiliation: MTA-BME Lendület Chemical Nanosensors Research Group, Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4, 1111 Budapest, Hungary.
authors.forename: Gergely
authors.initials: G
authors.lastname: Lautner
authors.affiliation: MTA-BME Lendület Chemical Nanosensors Research Group, Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4, 1111 Budapest, Hungary.
authors.forename: Róbert E
authors.initials: RE
authors.lastname: Gyurcsányi
authors.affiliation: Department of Medical Chemistry, University of Szeged, Dóm tér 8, 6720 Szeged, Hungary.
authors.forename: Titanilla
authors.initials: T
authors.lastname: Szögi
authors.affiliation: Department of Medical Chemistry, University of Szeged, Dóm tér 8, 6720 Szeged, Hungary. Electronic address: fulop.livia@med.u-szeged.hu.
authors.forename: Lívia
authors.initials: L
authors.lastname: Fülöp
authors.affiliation: Institute of Pharmaceutical Analysis, University of Szeged, Somogyi u. 4, 6720 Szeged, Hungary. Electronic address: martinek@pharm.u-szeged.hu.
authors.forename: Tamás A
authors.initials: TA
authors.lastname: Martinek
mesh_terms: D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D008958:Models, Molecular; D066329:Protein Aggregates; D000072756:Protein Conformation, alpha-Helical; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D013997:Time Factors
chemical_list: D016229:Amyloid beta-Peptides; D066329:Protein Aggregates
keywords: Antibody mimetics; Bioaffinity assay; Foldamers; Molecular recognition; β-Amyloid oligomers