title: Transplantation with hypoxia-preconditioned mesenchymal stem cells suppresses brain injury caused by cardiac arrest-induced global cerebral ischemia in rats.
abstract: Cardiac arrest-induced global cerebral ischemia is a main cause of neurological dysfunction in emergency medicine. Transplantation with bone marrow mesenchymal stem cells (MSCs) has been used in stroke models to repair the ischemic brain injury, but it is little studied in models with global cerebral ischemia. In the present study, a hypoxia precondition was used to improve the efficacy of MSC transplantation, given the low survival and migration rates and limited differentiation capacities of MSCs. We found that hypoxia can increase the expansion and migration of MSCs by activating the PI3K/AKT and hypoxia-inducible factor-1α/CXC chemokine receptor-4 pathways. By using a cardiac arrest-induced global cerebral ischemic model in rats, we found that transplantation of hypoxia-preconditioned MSCs promoted the migration and integration of MSCs and decreased neuronal death and inflammation in the ischemic cortex. © 2017 Wiley Periodicals, Inc.
authors.affiliation: Department of Intensive Care Unit, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guang Zhou, China.|Institute of Cardiopulmonary Cerebral Resuscitation, Sun Yat-Sen University, Guang Zhou, China.
authors.forename: Ji-Wen
authors.initials: JW
authors.lastname: Wang
authors.affiliation: Department of Intensive Care Unit, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guang Zhou, China.
authors.forename: Yu-Ru
authors.initials: YR
authors.lastname: Qiu
authors.affiliation: Department of Emergency Medicine, First People's Hospital of Fo Shan, Fo Shan, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Department of Neurology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guang Zhou, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Intensive Care Unit, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guang Zhou, China.
authors.forename: Zhi-Jie
authors.initials: ZJ
authors.lastname: He
authors.affiliation: Institute of Cardiopulmonary Cerebral Resuscitation, Sun Yat-Sen University, Guang Zhou, China.|Department of Emergency Medicine, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guang Zhou, China.
authors.forename: Zi-Tong
authors.identifier: 0000-0002-9954-9567
authors.initials: ZT
authors.lastname: Huang
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000209:etiology / Q000473:pathology*; D002545:Brain Ischemia / Q000209:etiology / Q000473:pathology*; D006323:Heart Arrest / Q000150:complications*; D000860:Hypoxia; D019194:Ischemic Preconditioning / Q000379:methods; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley
keywords: CXCR4; HIF; PI3K/AKT pathway; cardiac arrest; cerebral ischemia; hypoxia; mesenchymal stem cell; migration; rat; transplantation