title: Adsorption of DNA binding proteins to functionalized carbon nanotube surfaces with and without DNA wrapping.
abstract: We examined the adsorption of DNA binding proteins on functionalized, single-walled carbon nanotubes (SWNTs). When SWNTs were functionalized with polyethylene glycol (PEG-SWNT), moderate adsorption of protein molecules was observed. In contrast, nanotubes functionalized with CONH2groups (CONH2-SWNT) exhibited very strong interactions between the CONH2-SWNT and DNA binding proteins. Instead, when these SWNT surfaces were wrapped with DNA molecules (thymine 30-mers), protein binding was a little decreased. Our results revealed that DNA wrapped PEG-SWNT was one of the most promising candidates to realize DNA nanodevices involving protein reactions on DNA-SWNT surfaces. In addition, the DNA binding protein RecA was more adhesive than single-stranded DNA binding proteins to the functionalized SWNT surfaces.
authors.affiliation: Biophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo, 1628601, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Ishibashi
authors.affiliation: Biophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo, 1628601, Japan.
authors.forename: Shusuke
authors.initials: S
authors.lastname: Oura
authors.affiliation: Biophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo, 1628601, Japan. meicun2006@163.com.
authors.forename: Kazuo
authors.identifier: http://orcid.org/0000-0002-8073-3903
authors.initials: K
authors.lastname: Umemura
mesh_terms: D000327:Adsorption; D004247:DNA / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000737:chemistry*; D008958:Models, Molecular; D008968:Molecular Conformation; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D011938:Rec A Recombinases / Q000737:chemistry; D013499:Surface Properties
chemical_list: D004268:DNA-Binding Proteins; D037742:Nanotubes, Carbon; D011092:Polyethylene Glycols; D004247:DNA; D011938:Rec A Recombinases
keywords: Atomic force microscopy; DNA; DNA binding proteins; Polyethylene glycol; Single-walled carbon nanotubes