title: New natural products identified by combined genomics-metabolomics profiling of marine Streptomyces sp. MP131-18.
abstract: Marine actinobacteria are drawing more and more attention as a promising source of new natural products. Here we report isolation, genome sequencing and metabolic profiling of new strain Streptomyces sp. MP131-18 isolated from marine sediment sample collected in the Trondheim Fjord, Norway. The 16S rRNA and multilocus phylogenetic analysis showed that MP131-18 belongs to the genus Streptomyces. The genome of MP131-18 isolate was sequenced, and 36 gene clusters involved in the biosynthesis of 18 different types of secondary metabolites were predicted using antiSMASH analysis. The combined genomics-metabolics profiling of the strain led to the identification of several new biologically active compounds. As a result, the family of bisindole pyrroles spiroindimicins was extended with two new members, spiroindimicins E and F. Furthermore, prediction of the biosynthetic pathway for unusual α-pyrone lagunapyrone isolated from MP131-18 resulted in foresight and identification of two new compounds of this family - lagunapyrones D and E. The diversity of identified and predicted compounds from Streptomyces sp. MP131-18 demonstrates that marine-derived actinomycetes are not only a promising source of new natural products, but also represent a valuable pool of genes for combinatorial biosynthesis of secondary metabolites.
authors.affiliation: Helmholtz-Institute for Pharmaceutical Research Saarland, Actinobacteria Metabolic Engineering Group, Saarbrücken, Germany.
authors.forename: Constanze
authors.initials: C
authors.lastname: Paulus
authors.affiliation: Helmholtz-Institute for Pharmaceutical Research Saarland, Actinobacteria Metabolic Engineering Group, Saarbrücken, Germany.
authors.forename: Yuriy
authors.initials: Y
authors.lastname: Rebets
authors.affiliation: Helmholtz-Institute for Pharmaceutical Research Saarland, Actinobacteria Metabolic Engineering Group, Saarbrücken, Germany.
authors.forename: Bogdan
authors.initials: B
authors.lastname: Tokovenko
authors.affiliation: Helmholtz-Institute for Pharmaceutical Research Saarland, Actinobacteria Metabolic Engineering Group, Saarbrücken, Germany.
authors.forename: Suvd
authors.initials: S
authors.lastname: Nadmid
authors.affiliation: Gause Institute of New Antibiotics, Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: Larisa P
authors.initials: LP
authors.lastname: Terekhova
authors.affiliation: Helmholtz-Institute for Pharmaceutical Research Saarland, Actinobacteria Metabolic Engineering Group, Saarbrücken, Germany.
authors.forename: Maksym
authors.initials: M
authors.lastname: Myronovskyi
authors.affiliation: Department of Biotechnology, Norwegian University of Science and Technology, Trondheim, Norway.|Department of Pharmacognosy, University of Vienna, Vienna, Austria.
authors.forename: Sergey B
authors.initials: SB
authors.lastname: Zotchev
authors.affiliation: Center for Biotechnology, Bielefeld University, Bielefeld, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Rückert
authors.affiliation: Department of Pharmacy - Center for Drug Research, University of Munich, Munich, Germany.
authors.forename: Simone
authors.initials: S
authors.lastname: Braig
authors.affiliation: Department of Pharmacy - Center for Drug Research, University of Munich, Munich, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Zahler
authors.affiliation: Center for Biotechnology, Bielefeld University, Bielefeld, Germany.
authors.forename: Jörn
authors.initials: J
authors.lastname: Kalinowski
authors.affiliation: Helmholtz-Institute for Pharmaceutical Research Saarland, Actinobacteria Metabolic Engineering Group, Saarbrücken, Germany.|Universität des Saarlandes, Pharmaceutical Biotechnology, Saarbrücken, Germany.
authors.forename: Andriy
authors.initials: A
authors.lastname: Luzhetskyy
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000737:chemistry; D059001:Aquatic Organisms / Q000235:genetics* / Q000378:metabolism*; D001688:Biological Products / Q000032:analysis*; D053898:Biosynthetic Pathways; D016680:Genome, Bacterial; D023281:Genomics; D007211:Indoles / Q000737:chemistry; D055432:Metabolomics; D058977:Molecular Sequence Annotation; D005810:Multigene Family; D010802:Phylogeny; D011758:Pyrroles / Q000737:chemistry; D064210:Secondary Metabolism / Q000235:genetics; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001688:Biological Products; D007211:Indoles; D011758:Pyrroles; C030374:indole