title: Identification of four cornua by ultra-performance liquid chromatography with time-of-flight mass spectrometry coupled with principal component analysis.
abstract: An ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry method coupled with principal component analysis was developed and applied to the identification of Cornu Antelopis, Cornu Bubali, Cornu Naemorhedi, and Cornu Bovis. The data obtained from the trypsin-digested samples were subjected to principal component analysis to classify these four cornua. Additionally, marker peptides of the cornua were determined by orthogonal partial least-squares discriminant analysis, and fragmentation tandem mass spectra of these marker peptides were evaluated. The results from this study indicate that the proposed method is reliable, and it has been successfully applied to the identification of variants of cornua commonly used in traditional Chinese medicine.
authors.affiliation: Institute for Control of Traditional Chinese Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, China.
authors.forename: Xian-Long
authors.initials: XL
authors.lastname: Cheng
authors.affiliation: Institute for Control of Traditional Chinese Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, China.|Department of Pharmacy, Anhui University of Chinese Medicine, Hefei, Anhui, China.
authors.forename: Qian-Qian
authors.initials: QQ
authors.lastname: Zhang
authors.affiliation: Institute for Control of Traditional Chinese Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, China.
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Li
authors.affiliation: Institute for Control of Traditional Chinese Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yan
authors.affiliation: Institute for Control of Traditional Chinese Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, China.
authors.forename: Kun-Zi
authors.initials: KZ
authors.lastname: Yu
authors.affiliation: Department of Pharmacy, Anhui University of Chinese Medicine, Hefei, Anhui, China.
authors.forename: Jian-Li
authors.initials: JL
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Li
authors.affiliation: Institute for Control of Traditional Chinese Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wei
authors.affiliation: Institute for Control of Traditional Chinese Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, China.
authors.forename: Shuang-Cheng
authors.initials: SC
authors.lastname: Ma
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006733:Horns / Q000737:chemistry*; D008419:Materia Medica / Q000032:analysis*; D008516:Medicine, Chinese Traditional; D025341:Principal Component Analysis; D053719:Tandem Mass Spectrometry
chemical_list: D008419:Materia Medica
keywords: cornua; liquid chromatography; marker peptides; mass spectrometry; principal component analysis