title: Posttranscriptional chemical labeling of RNA by using bioorthogonal chemistry.
abstract: Recent developments in RNA labeling technology have provided viable tools to analyze RNA synthesis, processing and function in cell-free and cellular environments. Notably, emerging methodologies based on posttranscriptional chemical labeling by using bioorthogonal chemistry have enabled the visualization and profiling of exogenous and endogenous RNA transcripts. In this review, we first give an overview of different RNA labeling strategies based on chemical as well as genetically encoded systems. Subsequently, we provided a detailed discussion on methodologies that have been developed to introduce various bioorthogonal reactive groups into RNA transcripts, which are compatible for posttranscriptional functionalization. Finally, the utility of these techniques in imaging and studying the dynamics of RNA production, distribution and decay in complex cellular environment is discussed.
authors.affiliation: Department of Chemistry, Indian Institute of Science Education and Research (IISER) Pune, Dr. Homi Bhabha Road, Pashan, Pune 411008, India.
authors.forename: Jerrin Thomas
authors.initials: JT
authors.lastname: George
authors.affiliation: Department of Chemistry, Indian Institute of Science Education and Research (IISER) Pune, Dr. Homi Bhabha Road, Pashan, Pune 411008, India. Electronic address: srivatsan@iiserpune.ac.in.
authors.forename: Seergazhi G
authors.initials: SG
authors.lastname: Srivatsan
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods; D002474:Cell-Free System; D057930:Click Chemistry / Q000379:methods*; D003300:Copper / Q000737:chemistry / Q000378:metabolism; D061565:Cycloaddition Reaction / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry; D017403:In Situ Hybridization / Q000379:methods; D018613:Microscopy, Confocal; D057054:Molecular Imaging / Q000379:methods; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D013059:Spectrum Analysis, Raman; D013194:Staining and Labeling / Q000379:methods*; D014158:Transcription, Genetic
chemical_list: D005456:Fluorescent Dyes; D012313:RNA; D003300:Copper
keywords: Bioorthogonal chemistry; Click reactions; Posttranscriptional modification; RNA imaging; RNA labeling