title: Fast Single-Cell Patterning for Study of Drug-Induced Phenotypic Alterations of HeLa Cells Using Time-of-Flight Secondary Ion Mass Spectrometry.
abstract: A facile single-cell patterning (ScP) method was developed and integrated with time-of-flight secondary ion mass spectrometry (TOF-SIMS) for the study of drug-induced cellular phenotypic alterations. Micropatterned poly(dimethylsiloxane) (PDMS) stencil film and centrifugation-assisted cell trapping were combined for the preparation of on-surface single-cell microarrays, which exhibited both high site occupancy (>90%) and single-cell resolution (>97%). TOF-SIMS is a surface-sensitive mass spectrometry and is increasingly utilized in biological studies. Here we demonstrated, for the first time, its successful application in high-throughput single-cell analysis. Drug-induced phenotypic alterations of HeLa cells in the early stage of apoptosis were investigated using TOF-SIMS. The major molecular sources of variations were analyzed by principle component analysis (PCA).
authors.affiliation: Department of Chemistry, ‡Division of Biomedical Engineering, §Materials Characterization and Preparation Facility, Department of Chemical and Biomolecular Engineering, and ∥Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology , Hong Kong, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Chemistry, ‡Division of Biomedical Engineering, §Materials Characterization and Preparation Facility, Department of Chemical and Biomolecular Engineering, and ∥Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology , Hong Kong, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Chemistry, ‡Division of Biomedical Engineering, §Materials Characterization and Preparation Facility, Department of Chemical and Biomolecular Engineering, and ∥Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology , Hong Kong, China.
authors.forename: Lu-Tao
authors.initials: LT
authors.lastname: Weng
authors.affiliation: Department of Chemistry, ‡Division of Biomedical Engineering, §Materials Characterization and Preparation Facility, Department of Chemical and Biomolecular Engineering, and ∥Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology , Hong Kong, China.
authors.forename: Mark
authors.initials: M
authors.lastname: Leung
authors.affiliation: Department of Chemistry, ‡Division of Biomedical Engineering, §Materials Characterization and Preparation Facility, Department of Chemical and Biomolecular Engineering, and ∥Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology , Hong Kong, China.
authors.forename: Xiaoxing
authors.initials: X
authors.lastname: Xing
authors.affiliation: Department of Chemistry, ‡Division of Biomedical Engineering, §Materials Characterization and Preparation Facility, Department of Chemical and Biomolecular Engineering, and ∥Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology , Hong Kong, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Fan
authors.affiliation: Department of Chemistry, ‡Division of Biomedical Engineering, §Materials Characterization and Preparation Facility, Department of Chemical and Biomolecular Engineering, and ∥Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology , Hong Kong, China.
authors.forename: Hongkai
authors.identifier: 0000-0002-7766-7888
authors.initials: H
authors.lastname: Wu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D002945:Cisplatin / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor / Q000295:instrumentation*; D004867:Equipment Design; D006367:HeLa Cells; D006801:Humans; D059010:Single-Cell Analysis / Q000295:instrumentation*; D018629:Spectrometry, Mass, Secondary Ion / Q000295:instrumentation*; D046888:Tissue Array Analysis / Q000295:instrumentation*
chemical_list: D000970:Antineoplastic Agents; D002945:Cisplatin