title: Dual Functional MicroRNA-186-5p Targets both FGF2 and RelA to Suppress Tumorigenesis of Glioblastoma Multiforme.
abstract: Glioblastoma multiforme (GBM) is one of the most malignant cancers. MicroRNAs (miRs) were reported to play important roles in GBM recently. However, the role of a novel miR-186-5p in GBM tumorigenesis is still elusive. Using bioinformatics, miR-186-5p was identified as potential regulators of both fibroblast growth factor (FGF)-2 and NF-κB subunit RelA. Luciferase reporter assay was used to confirm the direct recognition FGF2 and RelA mRNAs by miR-186-5p. Invasion and migration assays were employed to study the effect of miR-186-5p on GBM cell growth in vitro. Xenograft tumor animal model was established to elucidate the in vivo function of miR-186-5p. MiR-186-5p directly targeted mRNAs of both FGF2 and RelA, and repressed their expressions. Invasive and migratory abilities of GBM cells and growth of xenograft tumors were significantly inhibited by miR-186-5p, which can be restored by re-introduction of FGF2 and RelA expressions. MiR-186-5p is a novel tumor suppressor miR that functions to inhibit tumorigenesis of GBM both in vitro and in vivo, by targeting both FGF2 and RelA. MiR-186-5p/FGF2/RelA pathway may be potentially used as molecular targets of in the clinical treatment of GBM.
authors.affiliation: Department of Neurosurgery, Yidu Central Hospital of Weifang, No. 4138 Linglongshan Road, Qingzhoushi, Shandong, People's Republic of China.
authors.forename: Fachen
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Medicine, Qingzhou Hospital of Traditional Chinese Medicine, No.2727 Haidai Road, Qingzhoushi, Shandong, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Neurosurgery, Yidu Central Hospital of Weifang, No. 4138 Linglongshan Road, Qingzhoushi, Shandong, People's Republic of China.
authors.forename: Shanjun
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, The Affiliated Hospital of Qingdao University, No. 1677 Wutaishan Road, Qingdao, Shandong, People's Republic of China. qyfychenbing@126.com.
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D063646:Carcinogenesis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D016222:Fibroblast Growth Factor 2 / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D016147:Genes, Tumor Suppressor / Q000502:physiology; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D051996:Transcription Factor RelA / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D023041:Xenograft Model Antitumor Assays / Q000379:methods
chemical_list: C533096:MIRN186 microRNA, human; D035683:MicroRNAs; C498143:RELA protein, human; D051996:Transcription Factor RelA; D016222:Fibroblast Growth Factor 2
keywords: Fibroblast growth factor (FGF)-2; Glioblastoma multiforme (GBM); MicroRNAs (miRs); Tumorigenesis