title: A Protein Isolate from Moringa oleifera Leaves Has Hypoglycemic and Antioxidant Effects in Alloxan-Induced Diabetic Mice.
abstract: Moringa oleiferahas been used in traditional medicine to treat diabetes. However, few studies have been conducted to relate its antidiabetic properties to proteins. In this study, a leaf protein isolate was obtained fromM. oleiferaleaves, namedMo-LPI, and the hypoglycemic and antioxidant effects on alloxan-induced diabetic mice were assessed.Mo-LPI was obtained by aqueous extraction, ammonium sulphate precipitation and dialysis. The electrophoresis profile and proteolytic hydrolysis confirmed its protein nature.Mo-LPI showed hemagglutinating activity, cross-reaction with anti-insulin antibodies and precipitation after zinc addition. Single-dose intraperitoneal (i.p.) administration ofMo-LPI (500 mg/kg·bw) reduced the blood glucose level (reductions of 34.3%, 60.9% and 66.4% after 1, 3 and 5 h, respectively). The effect ofMo-LPI was also evidenced in the repeated dose test with a 56.2% reduction in the blood glucose level on the 7th day after i.p. administration.Mo-LPI did not stimulate insulin secretion in diabetic mice.Mo-LPI was also effective in reducing the oxidative stress in diabetic mice by a decrease in malondialdehyde level and increase in catalase activity.Mo-LPI (2500 mg/kg·bw) did not cause acute toxicity to mice.Mo-LPI is a promising alternative or complementary agent to treat diabetes.
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60440-900, Brazil. paulolevemir@gmail.com.
authors.forename: Paulo C
authors.initials: PC
authors.lastname: Paula
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60440-900, Brazil. danisilas@yahoo.com.br.
authors.forename: Daniele O B
authors.initials: DO
authors.lastname: Sousa
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60440-900, Brazil. jtaolive@ufc.br.
authors.forename: Jose T A
authors.initials: JT
authors.lastname: Oliveira
authors.affiliation: Department of Biology, Federal University of Ceara, Fortaleza 60440-900, Brazil. aurano@ufc.br.
authors.forename: Ana F U
authors.initials: AF
authors.lastname: Carvalho
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60440-900, Brazil. bellaagiselly@gmail.com.
authors.forename: Bella G T
authors.initials: BG
authors.lastname: Alves
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60440-900, Brazil. mirellasally@gmail.com.
authors.forename: Mirella L
authors.initials: ML
authors.lastname: Pereira
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60440-900, Brazil. davi@dbm.ufpb.br.|Department of Molecular Biology, Federal University of Paraiba, Joao Pessoa 58051-900, Brazil. davi@dbm.ufpb.br.
authors.forename: Davi F
authors.initials: DF
authors.lastname: Farias
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60440-900, Brazil. vianamp@hotmail.com.
authors.forename: Martonio P
authors.initials: MP
authors.lastname: Viana
authors.affiliation: Department of Physiology and Pharmacology, Federal University of Ceara, Fortaleza 60430-160, Brazil. flavia@ufc.br.
authors.forename: Flavia A
authors.initials: FA
authors.lastname: Santos
authors.affiliation: Department of Physiology and Pharmacology, Federal University of Ceara, Fortaleza 60430-160, Brazil. talita.cm@hotmail.com.
authors.forename: Talita C
authors.initials: TC
authors.lastname: Morais
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60440-900, Brazil. imvasco@ufc.br.
authors.forename: Ilka M
authors.initials: IM
authors.lastname: Vasconcelos
mesh_terms: D000496:Alloxan / Q000009:adverse effects; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001786:Blood Glucose / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism; D006384:Hemagglutination / Q000187:drug effects; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000494:pharmacology*; D007328:Insulin / Q000097:blood; D029588:Moringa oleifera / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry / Q000494:pharmacology*; D011817:Rabbits
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D007004:Hypoglycemic Agents; D007328:Insulin; D010936:Plant Extracts; D010940:Plant Proteins; D000496:Alloxan
keywords: Moringa; antioxidant activity; diabetes therapy; hypoglycemic activity; plant protein