title: Investigation of silicon carbon nitride nanocomposite films as a wear resistant layer in vitro and in vivo for joint replacement applications.
abstract: Silicon-contained CNxnanocomposite films were prepared using the ion beam assisted magnetron sputtering under different nitrogen gas pressure. With increase of the nitrogen pressure, silicon and nitrogen content of the CNxfilms drastically increase, and is saturated as the PN2reach about 40%. Surface roughness and the contact angle are increase, while the friction coefficient decreased. The CNxfilm with 5.7at.% Si content possess the lowest friction coefficient of only 0.07, and exhibited the best tribological properties. The impact of CNxfilms with different silicon content on the growth and the activation of osteoblasts were compared to that of Ti6Al4V. The incorporation of silicon in the CNxfilm also showed an increase cell adhesion. Bonding structure and surface energy were determined to be the factors contributing to the improved biocompatibility. Macrophages attached to 5.7at.% Si contained CNxfilms down regulated their production of cytokines and chemokines. Moreover, employed with Si contained CNxcoated joint replacements, which were implanted subcutaneously into Sprague-Dawley mice for up to 36days, the tissue reaction and capsule formation was significantly decreased compared to that of Ti6Al4V. A mouse implantation study demonstrated the excellent in vivo biocompatibility and functional reliability of wear resist layer for joint replacements with a Si doped a-CNxcoating for 36days.
authors.affiliation: Center of Medical Device Adverse Events Monitoring of Anhui, Center for Adverse Drug Reaction Monitoring of Anhui, Hefei 230031, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Institute of Industry and Equipment Technology, School of Materials Science and Engineering, Hefei University of Technology, Hefei 230099, China; Center of Composite Material and Surface Treatment, China Electronic Technology Group Corporation No. 38 Research Institute (CETC 38), Hefei 230088, China. Electronic address: liudongguang2007@163.com.
authors.forename: D G
authors.initials: DG
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Silicon Materials and Department of Materials Science and Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: W Q
authors.initials: WQ
authors.lastname: Bai
authors.affiliation: State Key Laboratory of Silicon Materials and Department of Materials Science and Engineering, Zhejiang University, Hangzhou 310027, China. Electronic address: tujp@zju.edu.cn.
authors.forename: J P
authors.initials: JP
authors.lastname: Tu
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002244:Carbon / Q000008:administration & dosage / Q000737:chemistry; D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D007279:Injections, Subcutaneous; D008264:Macrophages / Q000378:metabolism; D008422:Materials Testing; D051379:Mice; D053761:Nanocomposites / Q000008:administration & dosage / Q000737:chemistry*; D009570:Nitriles / Q000008:administration & dosage / Q000737:chemistry; D009584:Nitrogen / Q000737:chemistry; D010316:Particle Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D012825:Silicon / Q000008:administration & dosage / Q000737:chemistry; D013499:Surface Properties; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D014867:Water / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D009570:Nitriles; D014409:Tumor Necrosis Factor-alpha; D014867:Water; D002244:Carbon; D009584:Nitrogen; D012825:Silicon
keywords: Cell viability; Hardness; Joint replacement; Surface roughness; Wear resistance