title: The β-barrel assembly machinery in motion.
abstract: In Gram-negative bacteria, the biogenesis of β-barrel outer membrane proteins (OMPs) is mediated by the β-barrel assembly machinery (BAM) complex. During the past decade, structural and functional studies have collectively contributed to advancing our understanding of the structure and function of the BAM complex; however, the exact mechanism that is involved remains elusive. In this Progress article, we discuss recent structural studies that have revealed that the accessory proteins may regulate essential unprecedented conformational changes in the core component BamA during function. We also detail the mechanistic insights that have been gained from structural data, mutagenesis studies and molecular dynamics simulations, and explore two emerging models for the BAM-mediated biogenesis of OMPs in bacteria.
authors.affiliation: Markey Center for Structural Biology, Department of Biological Sciences and the Purdue Institute for Inflammation, Immunology and Infectious Disease, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Noinaj
authors.affiliation: School of Physics, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.
authors.forename: James C
authors.initials: JC
authors.lastname: Gumbart
authors.affiliation: National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892, USA.
authors.forename: Susan K
authors.initials: SK
authors.lastname: Buchanan
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism / Q000648:ultrastructure*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D058635:Lipid-Linked Proteins / Q000378:metabolism*; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D000072417:Protein Domains; D017510:Protein Folding; D017433:Protein Structure, Secondary
chemical_list: D001425:Bacterial Outer Membrane Proteins; C503914:BamA protein, E coli; C557407:BamB protein, E coli; C556681:BamC protein, E coli; C513739:BamD protein, E coli; D029968:Escherichia coli Proteins; D058635:Lipid-Linked Proteins