title: Enhanced Peroxidase-Like Performance of Gold Nanoparticles by Hot Electrons.
abstract: Enzyme mimics have been widely used as alternatives to natural enzymes. However, the catalytic performances of enzyme mimics are often decreased due to different spatial structures or absence of functional groups compared to natural enzymes. Here, we report a highly efficient enzyme-like catalytic performance of gold nanoparticles (AuNPs) by visible-light stimulation. The enzyme-like reaction is evaluated by the catalytic reaction of AuNPs oxidizing a typical chromogenic substrate 3,3',5,5'-tetramethylbenzydine (TMB) with hydrogen peroxide as an oxidant. From investigations of the wavelength-dependent reaction rate, radical capture, hole-donor addition, and dark-field scattering spectroscopy experiments, it is revealed that the strong plasmonic absorption of AuNPs facilitates generation of hot electrons, which are transfered from AuNPs to the adsorbed reactant molecule, greatly promoting the catalytic performance of the enzyme-like catalytic reaction. The present work provides a simple method for improving the performance of enzyme mimics, which is expected to find further application in the field of plasmon-enhanced biocatalysis and biosensors.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, P. R. China.|Key Laboratory of Biomedical Functional Materials, School of Science, China Pharmaceutical University, Nanjing, 211198, P. R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, P. R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, P. R. China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Dan
authors.affiliation: Key Laboratory of Biomedical Functional Materials, School of Science, China Pharmaceutical University, Nanjing, 211198, P. R. China.
authors.forename: Xing-Guo
authors.initials: XG
authors.lastname: Nie
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, P. R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, P. R. China.
authors.forename: Xing-Hua
authors.initials: XH
authors.lastname: Xia
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D015374:Biosensing Techniques; D002384:Catalysis; D004583:Electrons; D006046:Gold / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D007700:Kinetics; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry*; D009195:Peroxidase / Q000737:chemistry / Q000378:metabolism; D013053:Spectrophotometry; D020349:Surface Plasmon Resonance
chemical_list: D001672:Biocompatible Materials; D006046:Gold; D006861:Hydrogen Peroxide; D009195:Peroxidase
keywords: enzyme mimics; gold nanoparticles; hot electrons; localized surface plasmon resonance; reaction mechanism