title: A Label-free and Turn-on Fluorescence Strategy for Kanamycin Detection Based on the NMM/G-quadruplex Structure.
abstract: In the present work, a simple design of a "label-free" fluorescence method for kanamycin was developed and explored based on a specific DNA aptamer as a recognizer and a NMM/G-quadruplex DNA system as a reporter. The titration experiment showed a linear detection range of 0.5 to 100 nM with a low detection limit of 0.5 nM. It also showed excellent selectivity in a selectivity experiment. It was then successfully employed to detect kanamycin in milk with a excellent reliability.
authors.affiliation: Jingchu Food Research and Development Center, College of Life Science, Yangtze University.
authors.forename: Hualin
authors.initials: H
authors.lastname: Yang
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Wu
authors.forename: Dongxiao
authors.initials: D
authors.lastname: Su
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Xingping
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D054856:G-Quadruplexes; D007612:Kanamycin / Q000032:analysis*; D057230:Limit of Detection; D008652:Mesoporphyrins / Q000737:chemistry*; D008892:Milk / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D008652:Mesoporphyrins; C065420:N-methylmesoporphyrin IX; D007612:Kanamycin; D004247:DNA