title: DNA methylation analysis of the EGR3 gene in patients of schizophrenia.
abstract: DNA methylation has been implicated in the pathogenesis of schizophrenia. EGR3 is considered as a potential candidate gene for schizophrenia. We conducted in vitro DNA methylation reaction, Lucia luciferase activity assay, and pyrosequencing assay to assess the DNA methylation of the EGR3 expression underlying the pathophysiology of schizophrenia. We found that DNA methylation of the putative EGR3 regulatory regions attenuated Lucia luciferase activity. There was no difference in the DNA methylation pattern of EGR3 between in 50 schizophrenic patients and 47 controls. Our data suggest that DNA methylation regulated the expression of EGR3 might not be associated with schizophrenia.
authors.affiliation: Department of Psychiatry, Yuli Mental Health Research Center, Yuli Branch, Taipei Veterans General Hospital, Hualien 981, Taiwan.
authors.forename: Tsung-Ming
authors.initials: TM
authors.lastname: Hu
authors.affiliation: Department of Psychiatry, Yuli Mental Health Research Center, Yuli Branch, Taipei Veterans General Hospital, Hualien 981, Taiwan.
authors.forename: Shih-Hsin
authors.initials: SH
authors.lastname: Hsu
authors.affiliation: Department of Psychiatry, Yuli Mental Health Research Center, Yuli Branch, Taipei Veterans General Hospital, Hualien 981, Taiwan.
authors.forename: Su-Min
authors.initials: SM
authors.lastname: Tsai
authors.affiliation: Department of Psychiatry, Yuli Mental Health Research Center, Yuli Branch, Taipei Veterans General Hospital, Hualien 981, Taiwan; Center for General Education, St. Mary's Junior College of Medicine, Nursing and Management, Yilan County, Taiwan. Electronic address: uncle055@yahoo.com.
authors.forename: Min-Chih
authors.initials: MC
authors.lastname: Cheng
mesh_terms: D019175:DNA Methylation / Q000235:genetics*; D051776:Early Growth Response Protein 3 / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D011401:Promoter Regions, Genetic / Q000235:genetics; D012559:Schizophrenia / Q000175:diagnosis* / Q000235:genetics*
chemical_list: C079945:EGR3 protein, human; D051776:Early Growth Response Protein 3
keywords: DNA methylation; EGR3; Schizophrenia