title: Ultrasensitive Self-Powered Aptasensor Based on Enzyme Biofuel Cell and DNA Bioconjugate: A Facile and Powerful Tool for Antibiotic Residue Detection.
abstract: Herein, we reported a novel ultrasensitive one-compartment enzyme biofuel cells (EBFCs)-based self-powered aptasensing platform for antibiotic residue detection. By taking full advantage of the unique features of both EBFCs-based self-powered sensors and aptamers, the as-proposed aptasensing platform has the merits of simple instrumentation, anti-interference ability, high selectivity, and low cost. In this study, DNA bioconjugate, i.e., SiO2@gold nanoparticles-complementary strand of aptamer (SiO2@AuNPs-csDNA), was elaborately designed and played a key role in blocking the mass transport of glucose to the bioanode. While in the presence of the target antibiotic, SiO2@AuNPs-csDNA bioconjugate broke away from the bioanode due to the aptamer recognition of the target. Without the blocking of glucose by the DNA bioconjugate, a significantly elevated open circuit voltage of the EBFCs-based aptasensor was obtained, whose amplitude was dependent on the antibiotic concentration. In addition, this proposed aptasensor was the first reported self-powered aptasensing platform for antibiotic determination and featured high sensitivity owing to the elaborate design of the DNA bioconjugate modified bioanode of EBFC, which was superior to those previously reported in the literature. Furthermore, due to the anti-interference ability and the excellent selectivity of the aptasensor, no special sample pretreatment was needed for the detection of antibiotics in milk samples. Therefore, the proposed EBFCs-based self-powered aptasensor has a great promise to be applied as a powerful tool for on-site assay in the field of food safety.
authors.affiliation: College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University , Qingdao 266109, People's Republic of China.
authors.forename: Panpan
authors.initials: P
authors.lastname: Gai
authors.affiliation: College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University , Qingdao 266109, People's Republic of China.
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Gu
authors.affiliation: College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University , Qingdao 266109, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Hou
authors.affiliation: College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University , Qingdao 266109, People's Republic of China.
authors.forename: Feng
authors.identifier: 0000-0002-3894-6139
authors.initials: F
authors.lastname: Li
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001674:Bioelectric Energy Sources; D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry*; D004350:Drug Residues / Q000032:analysis*; D004566:Electrodes; D004798:Enzymes / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D052157:Aptamers, Nucleotide; D004798:Enzymes; D006046:Gold; D012822:Silicon Dioxide; D004247:DNA; D005947:Glucose