title: A simple, portable, electrochemical biosensor to screen shellfish for Vibrio parahaemolyticus.
abstract: An earlier electrochemical mechanism of DNA detection was adapted and specified for the detection of Vibrio parahaemolyticus in real samples. The reader, based on a screen printed carbon electrode, was modified with polylactide-stabilized gold nanoparticles and methylene blue was employed as the redox indicator. Detection was assessed using a microprocessor to measure current response under controlled potential. The fabricated sensor was able to specifically distinguish complementary, non-complementary and mismatched oligonucleotides. DNA was measured in the range of 2.0 × 10-8-2.0 × 10-13M with a detection limit of 2.16 pM. The relative standard deviation for 6 replications of differential pulse voltammetry (DPV) measurement of 0.2 µM complementary DNA was 4.33%. Additionally, cross-reactivity studies against various other food-borne pathogens showed a reliably sensitive detection of the target pathogen. Successful identification of Vibrio parahaemolyticus (spiked and unspiked) in fresh cockles, combined with its simplicity and portability demonstrate the potential of the device as a practical screening tool.
authors.affiliation: Institute of Advanced Technology, Universiti Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia.|Food Safety Research Centre, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
authors.forename: Noordiana
authors.initials: N
authors.lastname: Nordin
authors.affiliation: Institute of Advanced Technology, Universiti Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia. azahy@upm.edu.my.|Department of Chemistry, Faculty of Science, Universiti Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia. azahy@upm.edu.my.
authors.forename: Nor Azah
authors.initials: NA
authors.lastname: Yusof
authors.affiliation: Institute of Advanced Technology, Universiti Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia.|Department of Chemistry, Faculty of Science, Universiti Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia.
authors.forename: Jaafar
authors.initials: J
authors.lastname: Abdullah
authors.affiliation: Food Safety Research Centre, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
authors.forename: Son
authors.initials: S
authors.lastname: Radu
authors.affiliation: La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia. r.hushiarian@imperial.ac.uk.|Department of Life Sciences, Imperial College London, London, SW7 2AZ, UK. r.hushiarian@imperial.ac.uk.
authors.forename: Roozbeh
authors.identifier: http://orcid.org/0000-0002-0403-7586
authors.initials: R
authors.lastname: Hushiarian
keywords: Electrochemical DNA sensor; Food-borne pathogens; Portable biosensor; Shellfish; Vibrio parahaemolyticus