title: Development and validation of the first SSR markers for Mimosa scabrella Benth.
abstract: Mimosa scabrella Benth., popularly known as ''bracatinga'', is a pioneer and endemic species of Brazil, occurring in Mixed Ombrophilous Forest associated with Brazilian Atlantic Rainforest biomes. It is a fast-growing tree of the Fabaceae family that facilitates the dynamics of ecological succession. SSR development, when there is no genome sequence, is time and labor intensive and there are no molecular markers for M. scabrella. We developed and validated the first microsatellite markers for this tetraploid species, evaluating mother trees and progenies. Using Illumina sequencing, we identified 290 SSR loci and 211 primer pairs. After 31 SSR loci PCR/agarose electrophoresis selection, a subset of 11 primer pairs was synthetized with fluorescence in the forward primer for PCR and capillary electrophoresis validation with leaf DNA of 33 adult and 411 progeny individuals. Polymorphic locus percentage was 36, 4 in 11 loci, 3 chloroplast SSRs, and 1 nuclear SSR. Allele number of polymorphic loci ranged from 2 to 11 alleles considering all sampling. All 11 primer pairs were also tested for cross-species amplification for five Fabaceae-Mimosoideae species, ranging from 2 loci transferred to Calliandra tweedii Benth. and all 11 loci transferred to Mimosa taimbensis Burkart. The assessed and validated SSR markers for M. scabrella are suitable and useful for analysis and population genetic studies.
authors.affiliation: Departamento de Agronomia, Universidade do Estado de Santa Catarina, Lages, SC, Brasil.
authors.forename: F A
authors.initials: FA
authors.lastname: Saiki
authors.affiliation: Departamento de Fitotecnia, Universidade Federal de Santa Catarina, Florianópolis, SC, Brasil.
authors.forename: A P
authors.initials: AP
authors.lastname: Bernardi
authors.affiliation: Departamento de Fitotecnia, Universidade Federal de Santa Catarina, Florianópolis, SC, Brasil.
authors.forename: M S
authors.initials: MS
authors.lastname: Reis
authors.affiliation: Instituto Carlos Chagas-Fundação Oswaldo Cruz, Curitiba, PR, Brasil.
authors.forename: H
authors.initials: H
authors.lastname: Faoro
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, PR, Brasil.
authors.forename: E M
authors.initials: EM
authors.lastname: Souza
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, PR, Brasil.
authors.forename: F O
authors.initials: FO
authors.lastname: Pedrosa
authors.affiliation: Departamento de Engenharia Florestal, Universidade do Estado de Santa Catarina, Lages, SC, Brasil.
authors.forename: A
authors.initials: A
authors.lastname: Mantovani
authors.affiliation: Departamento de Agronomia, Universidade do Estado de Santa Catarina, Lages, SC, Brasil.
authors.forename: A F
authors.initials: AF
authors.lastname: Guidolin
mesh_terms: D002467:Cell Nucleus / Q000235:genetics; D002736:Chloroplasts / Q000235:genetics; D017931:DNA Primers / Q000138:chemical synthesis*; D005819:Genetic Markers; D018895:Microsatellite Repeats; D031298:Mimosa / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods; D013045:Species Specificity; D057891:Tetraploidy
chemical_list: D017931:DNA Primers; D005819:Genetic Markers