title: High hydrostatic pressure effects on Listeria monocytogenes and L. innocua: Evidence for variability in inactivation behaviour and in resistance to pediocin bacHA-6111-2.
abstract: The effect of high hydrostatic pressure (HHP) on the survival of 14 strains of Listeria monocytogenes from food or clinical origins, selected to represent different pheno and genotypes, was evaluated. Stationary phase cells were submitted to 300, 400 and 500 MPa at 10 °C, for 5 min. A high variability in the resistance of L. monocytogenes to pressure was observed, and particularly two strains isolated from food were significantly more baroresistant than the rest. Strains of L. monocytogenes resistant to one or more antibiotics exhibited significantly higher levels of survival after the high pressure treatment at 400 MPa. No correlation was found between strains' origin or thermal tolerance and resistance to HHP. The suitability of two strains of L. innocua as surrogates of L. monocytogenes, was also investigated. These exhibited significantly higher sensitivities to HHP than observed for some L. monocytogenes. The antimicrobial effect of the antilisterial bacteriocin (bacHA-6111-2) increased after L. monocytogenes cells had been exposed to pressure. The data obtained underlines the importance of strain selection for studies aiming to evaluate HHP efficacy to ensure safety of HHP-treated foods.
authors.affiliation: CBQF - Centro de Biotecnologia e Química Fina, Escola Superior de Biotecnologia, Centro Regional do Porto da Universidade Católica Portuguesa, Rua Arquiteto Lobão Vital Apartado 2511, 4202-401 Porto, Portugal.
authors.forename: Carolina
authors.initials: C
authors.lastname: Bruschi
authors.affiliation: CBQF - Centro de Biotecnologia e Química Fina, Escola Superior de Biotecnologia, Centro Regional do Porto da Universidade Católica Portuguesa, Rua Arquiteto Lobão Vital Apartado 2511, 4202-401 Porto, Portugal.
authors.forename: Norton
authors.initials: N
authors.lastname: Komora
authors.affiliation: CBQF - Centro de Biotecnologia e Química Fina, Escola Superior de Biotecnologia, Centro Regional do Porto da Universidade Católica Portuguesa, Rua Arquiteto Lobão Vital Apartado 2511, 4202-401 Porto, Portugal; QOPNA, Departamento de Química, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
authors.forename: Sónia Marília
authors.initials: SM
authors.lastname: Castro
authors.affiliation: QOPNA, Departamento de Química, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
authors.forename: Jorge
authors.initials: J
authors.lastname: Saraiva
authors.affiliation: CBQF - Centro de Biotecnologia e Química Fina, Escola Superior de Biotecnologia, Centro Regional do Porto da Universidade Católica Portuguesa, Rua Arquiteto Lobão Vital Apartado 2511, 4202-401 Porto, Portugal.
authors.forename: Vânia Borges
authors.initials: VB
authors.lastname: Ferreira
authors.affiliation: CBQF - Centro de Biotecnologia e Química Fina, Escola Superior de Biotecnologia, Centro Regional do Porto da Universidade Católica Portuguesa, Rua Arquiteto Lobão Vital Apartado 2511, 4202-401 Porto, Portugal. Electronic address: pcteixeira@porto.ucp.pt.
authors.forename: Paula
authors.initials: P
authors.lastname: Teixeira
mesh_terms: D015169:Colony Count, Microbial; D005506:Food Contamination / Q000517:prevention & control; D005516:Food Microbiology; D059022:Food Safety; D006874:Hydrostatic Pressure; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology*; D008088:Listeriosis / Q000382:microbiology; D050296:Microbial Viability; D000070017:Pediocins / Q000494:pharmacology*; D000071436:Thermotolerance
chemical_list: D000070017:Pediocins
keywords: High hydrostatic pressure (HHP); Listeria monocytogenes; Pediocin; Strain variability