title: Spectroscopic study of honey from Apis mellifera from different regions in Mexico.
abstract: The objective of this study was to analyze by Raman and UV-Vis-NIR Spectroscopic techniques, Mexican honey from Apis Mellífera, using representative samples with different botanic origins (unifloral and multifloral) and diverse climates. Using Raman spectroscopy together with principal components analysis, the results obtained represent the possibility to use them for determination of floral origin of honey, independently of the region of sampling. For this, the effect of heat up the honey was analyzed in relation that it was possible to greatly reduce the fluorescence background in Raman spectra, which allowed the visualization of fructose and glucose peaks. Using UV-Vis-NIR, spectroscopy, a characteristic spectrum profile of transmittance was obtained for each honey type. In addition, to have an objective characterization of color, a CIE Yxy and CIE L*a*b* colorimetric register was realized for each honey type. Applying the principal component analysis and their correlation with chromaticity coordinates allowed classifying the honey samples in one plot as: cutoff wavelength, maximum transmittance, tones and lightness. The results show that it is possible to obtain a spectroscopic record of honeys with specific characteristics by reducing the effects of fluorescence.
authors.affiliation: Centro de Investigaciones en Óptica, A.C., Unidad Aguascalientes, Mexico. Electronic address: cfraus@cio.mx.
authors.forename: C
authors.initials: C
authors.lastname: Frausto-Reyes
authors.affiliation: Universidad Autónoma de Aguascalientes, Departamento de Tecnología de Alimentos, Mexico. Electronic address: racasi@correo.uaa.mx.
authors.forename: R
authors.initials: R
authors.lastname: Casillas-Peñuelas
authors.affiliation: Universidad Autónoma de Aguascalientes, Departamento de Fisiología y Farmacología, Centro de Ciencias Básicas, Mexico.
authors.forename: J L
authors.initials: JL
authors.lastname: Quintanar-Stephano
authors.affiliation: Centro de Investigaciones en Óptica, A.C., Unidad Aguascalientes, Mexico.
authors.forename: E
authors.initials: E
authors.lastname: Macías-López
authors.affiliation: Centro de Investigaciones en Óptica, A.C., Unidad Aguascalientes, Mexico.
authors.forename: J M
authors.initials: JM
authors.lastname: Bujdud-Pérez
authors.affiliation: Universidad Autónoma de Aguascalientes, Departamento de Química, Mexico.
authors.forename: I
authors.initials: I
authors.lastname: Medina-Ramírez
mesh_terms: D000818:Animals; D001516:Bees / Q000737:chemistry*; D003124:Colorimetry; D035264:Flowers / Q000737:chemistry; D005632:Fructose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006722:Honey / Q000032:analysis*; D008800:Mexico; D025341:Principal Component Analysis; D013056:Spectrophotometry, Ultraviolet; D019265:Spectroscopy, Near-Infrared; D013059:Spectrum Analysis, Raman
chemical_list: D005632:Fructose; D005947:Glucose
keywords: Principal component analysis; Raman spectroscopy; Spectrocolorimetry; UV–Vis-NIR spectroscopy; Unifloral and multifloral honey from Apis mellifera