title: Compositions and Abundances of Sulfate-Reducing and Sulfur-Oxidizing Microorganisms in Water-Flooded Petroleum Reservoirs with Different Temperatures in China.
abstract: Sulfate-reducing bacteria (SRB) have been studied extensively in the petroleum industry due to their role in corrosion, but very little is known about sulfur-oxidizing bacteria (SOB), which drive the oxidization of sulfur-compounds produced by the activity of SRB in petroleum reservoirs. Here, we surveyed the community structure, diversity and abundance of SRB and SOB simultaneously based on 16S rRNA,dsrBandsoxBgene sequencing, and quantitative PCR analyses, respectively in petroleum reservoirs with different physicochemical properties. Similar to SRB, SOB were found widely inhabiting the analyzed reservoirs with high diversity and different structures. The dominant SRB belonged to the classesDeltaproteobacteriaandClostridia, and included theDesulfotignum,Desulfotomaculum,Desulfovibrio,Desulfobulbus, andDesulfomicrobiumgenera. The most frequently detected potential SOB wereSulfurimonas,Thiobacillus,Thioclava,ThiohalomonasandDechloromonas, and belonged toBetaproteobacteria,Alphaproteobacteria, andEpsilonproteobacteria. Among them,Desulfovibrio,Desulfomicrobium,Thioclava, andSulfurimonaswere highly abundant in the low-temperature reservoirs, whileDesulfotomaculum,Desulfotignum, Thiobacillus, andDechloromonaswere more often present in high-temperature reservoirs. The relative abundances of SRB and SOB varied and were present at higher proportions in the relatively high-temperature reservoirs. Canonical correspondence analysis also revealed that the SRB and SOB communities in reservoirs displayed high niche specificity and were closely related to reservoir temperature, pH of the formation brine, and sulfate concentration. In conclusion, this study extends our knowledge about the distribution of SRB and SOB communities in petroleum reservoirs.
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University Tianjin, China.
authors.forename: Huimei
authors.initials: H
authors.lastname: Tian
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University Tianjin, China.
authors.forename: Peike
authors.initials: P
authors.lastname: Gao
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University Tianjin, China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University Tianjin, China.
authors.forename: Yanshu
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University Tianjin, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University Tianjin, China.
authors.forename: Yansen
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University Tianjin, China.
authors.forename: Jiefang
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University Tianjin, China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Li
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University Tianjin, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Ma
keywords: abundance; corrosion; diversity; oil reservoirs; sulfate-reducing bacteria (SRB); sulfur-oxidizing bacteria (SOB)