title: Phosphosite-Specific Antibodies: A Brief Update on Generation and Applications.
abstract: Phosphate addition is a posttranslational modification of proteins, and this modification can affect the activity and other properties of intracellular proteins. Different animal species can be used to generate phosphosite-specific antibodies as either polyclonals or monoclonals, and each approach offers its own benefits and disadvantages. The validation of phosphosite-specific antibodies requires multiple techniques and tactics to demonstrate their specificity. These antibodies can be used in arrays, flow cytometry, and imaging platforms. The specificity of phosphosite-specific antibodies is vital for their use in proteomics and profiling of disease.
authors.affiliation: Bio-Techne, Inc., 614 McKinley Place NE, Minneapolis, MN, 55413, USA. Kathy.brumbaugh@bio-techne.com.
authors.forename: Kathy
authors.initials: K
authors.lastname: Brumbaugh
authors.affiliation: Bio-Techne, Inc., 614 McKinley Place NE, Minneapolis, MN, 55413, USA.
authors.forename: Wen-Chie
authors.initials: WC
authors.lastname: Liao
authors.affiliation: Bio-Techne, Inc., 614 McKinley Place NE, Minneapolis, MN, 55413, USA.
authors.forename: J P
authors.initials: JP
authors.lastname: Houchins
authors.affiliation: Bio-Techne, Inc., 614 McKinley Place NE, Minneapolis, MN, 55413, USA.
authors.forename: Jeff
authors.initials: J
authors.lastname: Cooper
authors.affiliation: Bio-Techne, Inc., 614 McKinley Place NE, Minneapolis, MN, 55413, USA.
authors.forename: Steve
authors.initials: S
authors.lastname: Stoesz
mesh_terms: D000596:Amino Acids / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000917:Antibody Formation; D000918:Antibody Specificity / Q000276:immunology*; D015153:Blotting, Western; D061505:Cell Surface Display Techniques; D055808:Drug Discovery / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D006801:Humans; D006825:Hybridomas / Q000276:immunology / Q000378:metabolism; D007150:Immunohistochemistry; D051379:Mice; D010750:Phosphoproteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D010766:Phosphorylation; D040081:Protein Array Analysis / Q000379:methods; D015203:Reproducibility of Results
chemical_list: D000596:Amino Acids; D000906:Antibodies; D010750:Phosphoproteins
keywords: Antibody; ELISA; Flow cytometry; Immunocytochemistry; Multiplex; Phosphosite-specific; Simple Western; Western blotting