title: Absolute Quantification of Bionanoparticles by Electrospray Differential Mobility Analysis: An Application to Lipoprotein Particle Concentration Measurements.
abstract: This study presents an upgraded electrospray differential mobility analysis (ES-DMA) setup for the absolute quantification of bionanoparticle concentrations in biological samples, with a special focus on non-high-density-lipoprotein particle concentrations (non-HDL-P). Metrological characterization of the system's analytical performances for concentration measurements shows that the mean intermediate precision relative standard deviation is 14% for biological samples, 6% for silica nanoparticles, and less than 1% for diameter measurements. This study also demonstrates that the most accurate method for non-HDL-P quantification in native serum samples implies daily calculation of the electrospray transmission efficiency (E) of the system with the WHO SP3-08 reference material. The establishment of the uncertainty budget reveals that the main contribution to particle concentration measurement uncertainties is the electrospray transmission efficiency. This data additionally shows that E is not only low (approximately 15-20%) but also highly variable over time and strongly affected by sample composition. This work suggests that absolute enumeration of bionanoparticles is achievable with ES-DMA but provided that a special care is taken to quantifying E with a calibrator of nature and matrix highly similar to the samples ones.
authors.affiliation: Laboratoire National de Métrologie et d'Essais, LNE , Chemistry and Biology Division, 1 rue Gaston Boissier, 75724 Paris Cedex 15, France.
authors.forename: Noémie
authors.identifier: 0000-0002-3966-2453
authors.initials: N
authors.lastname: Clouet-Foraison
authors.affiliation: Laboratoire National de Métrologie et d'Essais, LNE , Chemistry and Biology Division, 1 rue Gaston Boissier, 75724 Paris Cedex 15, France.
authors.forename: Francois
authors.identifier: 0000-0002-3960-1642
authors.initials: F
authors.lastname: Gaie-Levrel
authors.affiliation: Laboratoire National de Métrologie et d'Essais, LNE , Chemistry and Biology Division, 1 rue Gaston Boissier, 75724 Paris Cedex 15, France.
authors.forename: Loic
authors.initials: L
authors.lastname: Coquelin
authors.affiliation: Laboratoire National de Métrologie et d'Essais, LNE , Chemistry and Biology Division, 1 rue Gaston Boissier, 75724 Paris Cedex 15, France.
authors.forename: Géraldine
authors.initials: G
authors.lastname: Ebrard
authors.affiliation: University of Reims Champagne-Ardenne, Faculty of Medicine, UMR CNRS/URCA n°7369 and University Hospital of Reims, Laboratory of Pediatric Biology and Research, 45 rue Cognacq-Jay, 51092 Reims Cedex, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Gillery
authors.affiliation: Laboratoire National de Métrologie et d'Essais, LNE , Chemistry and Biology Division, 1 rue Gaston Boissier, 75724 Paris Cedex 15, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Delatour