title: Analysis of Long Non-Coding RNA Expression of Lymphatic Endothelial Cells in Response to Type 2 Diabetes.
abstract: BACKGROUND
  Recent evidence has indicated that long non-coding RNA (lncRNA) is involved in the pathogenesis of type 2 diabetes, but nothing is known about lncRNA expression changes of lymphatic endothelial cells in response to type 2 diabetes.
  
  
  METHODS
  The GSE38396 dataset was downloaded from the Gene Expression Omnibus database and the probe sets of Human Gnome U133 Plus2.0 microarray were annotated for lncRNA. Differentially expressed lncRNAs between diabetic and non-diabetic lymphatic endothelial cells were calculated.
  
  
  RESULTS
  Compared with lymphatic endothelial cells in non-diabetic patients, 31 lncRNAs were down-regulated and 79 lncRNAs were up-regualted in lymphatic endothelial cells of type 2 diabetic patients. Several known lncRNAs were found, such as H19, GAS5, UCA1, CRNDE, GAS5, and LINC00312. Co-expression network of differentially expressed lncRNAs and mRNAs were constructed. Based on genomic regions of these lncRNAs, we found that binding sites of MAF and TCF3 were enriched and these lncRNAs may be related to insulin reporter signaling pathway and response to insulin stimulus.
  
  
  CONCLUSIONS
  In a word, we found a set of lncRNAs were differentially expressed in lymphatic endothelial cells in response to type 2 diabetes and these lncRNAs may be involved in the pathogenesis of diabetes-related complications.
authors.forename: Min
authors.initials: M
authors.lastname: Qi
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Zhou
authors.forename: Weiqi
authors.initials: W
authors.lastname: Zeng
authors.forename: Minxue
authors.initials: M
authors.lastname: Shen
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Liu
authors.forename: Chang
authors.initials: C
authors.lastname: Luo
authors.forename: Jing
authors.initials: J
authors.lastname: Long
authors.forename: Wangqing
authors.initials: W
authors.lastname: Chen
authors.forename: Jianglin
authors.initials: J
authors.lastname: Zhang
authors.forename: Siyu
authors.initials: S
authors.lastname: Yan
mesh_terms: D030541:Databases, Genetic; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics / Q000473:pathology*; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D053263:Gene Regulatory Networks; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D062085:RNA, Long Noncoding / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D062085:RNA, Long Noncoding; D012333:RNA, Messenger
keywords: H19; Long non-coding RNA; Lymphatic endothelial cells; Type 2 diabetes