title: Effect of different enrichment strategies on microbial community structure in petroleum-contaminated marine sediment in Dalian, China.
abstract: An oil spill occurred at Xingang Port, Dalian, China in 2010. Four years after this spill, oil contamination was still detected in samples collected nearby. In this study, the strains that evolved in the sediment were screened by high-throughput sequencing technology. Most of these strains were genera reported to have functions associated with crude oil biodegradation. The diversities and numbers of microbes were monitored through enrichment culturing; the dominant strains propagated at first, but the enrichment could not be continued, which indicated that the prolonged culture was not effective in the enrichment of the micro-consortium. Oxygen was also observed to affect the propagation of the dominant microbes. The results showed the role of culture strategies and oxygen in the enrichment of the petroleum-degrading microbes. Therefore, dominant strains could be screened by optimizing both the enrichment time and oxygen concentration used for culturing to facilitate oil biodegradation in the marine ecosystem.
authors.affiliation: College of Life Science, Dalian Nationalities University, Dalian 116600, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.affiliation: College of Life Science, Dalian Nationalities University, Dalian 116600, PR China. Electronic address: liuqiu@dlnu.edu.cn.
authors.forename: Qiu
authors.initials: Q
authors.lastname: Liu
authors.affiliation: College of Life Science, Dalian Nationalities University, Dalian 116600, PR China.
authors.forename: Changjian
authors.initials: C
authors.lastname: Liu
authors.affiliation: College of Life Science, Dalian Nationalities University, Dalian 116600, PR China. Electronic address: yujc@dlnu.edu.cn.
authors.forename: Jicheng
authors.initials: J
authors.lastname: Yu
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002681:China; D017753:Ecosystem; D019015:Geologic Sediments / Q000382:microbiology*; D010578:Petroleum / Q000378:metabolism*; D059392:Petroleum Pollution; D012623:Seawater / Q000737:chemistry / Q000382:microbiology; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000378:metabolism
chemical_list: D010578:Petroleum; D014874:Water Pollutants, Chemical
keywords: Enrichment time; Marine sediment; Oxygen; Petroleum contamination