title: Highly predictive SNP markers for efficient selection of the wheat leaf rust resistance gene Lr16.
abstract: Lr16 is a widely deployed leaf rust resistance gene in wheat (Triticum aestivum L.) that is highly effective against the North American Puccinia triticina population when pyramided with the gene Lr34. Lr16 is a seedling leaf rust resistance gene conditioning an incompatible interaction with a distinct necrotic ring surrounding the uredinium. Lr16 was previously mapped to the telomeric region of the short arm of wheat chromosome 2B. The goals of this study were to develop numerous single nucleotide polymorphism (SNP) markers for the Lr16 region and identify diagnostic gene-specific SNP marker assays for marker-assisted selection (MAS).
  
  
  
  Forty-three SNP markers were developed and mapped on chromosome 2BS tightly linked with the resistance gene Lr16 across four mapping populations representing a total of 1528 gametes. Kompetitive Allele Specific PCR (KASP) assays were designed for all identified SNPs. Resistance gene analogs (RGAs) linked with the Lr16 locus were identified and RGA-based SNP markers were developed. The diagnostic potential of the SNPs co-segregating with Lr16 was evaluated in a diverse set of 133 cultivars and breeding lines. Six SNP markers were consistent with the Lr16 phenotype and are accurately predictive of Lr16 for all wheat lines/cultivars in the panel.
  
  
  
  Lr16 was mapped relative to SNP markers in four populations. Six SNP markers exhibited high quality clustering in the KASP assay and are suitable for MAS of Lr16 in wheat breeding programs.
authors.affiliation: Agriculture and Agri-Food Canada, Morden Research and Development Centre, 101 Route 100, Morden, MB, R6M 1Y5, Canada.|National Research Council, 110 Gymnasium Place, Saskatoon, SK, S7N 0W9, Canada.
authors.forename: Mulualem T
authors.initials: MT
authors.lastname: Kassa
authors.affiliation: Agriculture and Agri-Food Canada, Morden Research and Development Centre, 101 Route 100, Morden, MB, R6M 1Y5, Canada.
authors.forename: Frank M
authors.initials: FM
authors.lastname: You
authors.affiliation: Agriculture and Agri-Food Canada, Morden Research and Development Centre, 101 Route 100, Morden, MB, R6M 1Y5, Canada.
authors.forename: Colin W
authors.initials: CW
authors.lastname: Hiebert
authors.affiliation: University of Saskatchewan, Crop Development Centre, 51 Campus Drive, Saskatoon, SK, S7N 5A8, Canada.
authors.forename: Curtis J
authors.initials: CJ
authors.lastname: Pozniak
authors.affiliation: National Research Council, 110 Gymnasium Place, Saskatoon, SK, S7N 0W9, Canada.
authors.forename: Pierre R
authors.initials: PR
authors.lastname: Fobert
authors.affiliation: National Research Council, 110 Gymnasium Place, Saskatoon, SK, S7N 0W9, Canada.|University of Saskatchewan, Global Institute for Food Security, 110 Gymnasium Place, Saskatoon, SK, S7N 4J8, Canada.
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Sharpe
authors.affiliation: Agriculture and Agri-Food Canada, Morden Research and Development Centre, 101 Route 100, Morden, MB, R6M 1Y5, Canada.
authors.forename: James G
authors.initials: JG
authors.lastname: Menzies
authors.affiliation: Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, 1341 Baseline Road, Ottawa, ON, K1A 0C5, Canada.
authors.forename: D Gavin
authors.initials: DG
authors.lastname: Humphreys
authors.affiliation: Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506, USA.
authors.forename: Nicole
authors.initials: N
authors.lastname: Rezac Harrison
authors.affiliation: USDA-ARS, Hard Winter Wheat Genetics Research Unit, Manhattan, KS, 66506, USA.
authors.forename: John P
authors.initials: JP
authors.lastname: Fellers
authors.affiliation: Agriculture and Agri-Food Canada, Morden Research and Development Centre, 101 Route 100, Morden, MB, R6M 1Y5, Canada.
authors.forename: Brent D
authors.initials: BD
authors.lastname: McCallum
authors.affiliation: Agriculture and Agri-Food Canada, Morden Research and Development Centre, 101 Route 100, Morden, MB, R6M 1Y5, Canada. Curt.McCartney@agr.gc.ca.
authors.forename: Curt A
authors.initials: CA
authors.lastname: McCartney
mesh_terms: D001487:Basidiomycota / Q000472:pathogenicity; D002874:Chromosome Mapping; D060467:Disease Resistance / Q000235:genetics; D005819:Genetic Markers; D006239:Haplotypes; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D036226:Seedlings / Q000235:genetics / Q000382:microbiology; D014908:Triticum / Q000235:genetics* / Q000382:microbiology*
chemical_list: D005819:Genetic Markers; D010940:Plant Proteins
keywords: Disease resistance; Leaf rust; Linkage analysis; Lr16; Puccinia triticina; Resistance gene analog (RGA); Single nucleotide polymorphism (SNP); Triticum aestivum L; Wheat