title: Pathological and biochemical evaluation of coumarin and chlorophyllin against aflatoxicosis in rat.
abstract: Aflatoxin contamination of animal diet has adverse effects on animal health and productivity. This study was performed to investigate the effect of using coumarin and/or chlorophyllin in rat diet against aflatoxicosis. Fifty-four rats were assigned into 7 groups (6 rats each). G1 was a negative control. G2 received water with coumarin 0.5%. G3 received water with chlorophyllin 0.5%. G4 received water with coumarin 0.5% and chlorophyllin 0.5%. G5-8 fed aflatoxin B11000ppb in diet. Group 6-8 were administered similar treatments as G2-4. The experiment ended after 8 weeks. Random glucose, total lipid, total cholesterol, total triglycerides, total protein, serum ALT, AST, creatinine, and urea were measured. Histopathology of liver, kidney and pancreas and immunohistochemical staining of placental glutathione-S-transferase (GST-P) in liver were performed. The glucose serum level, cholesterol, AST, and ALT were elevated in G5 compared to G6-8. The liver and kidney lesions in G5 included vacuolation and necrosis which subsided in G6-8. The necrosis and inflammatory cells infiltration in the pancreas of G5 were absent in G6-8. GST-P positive hepatocytes were abundant in G5, few in G6 and absent in G7 and G8. In conclusion, the chlorophyllin and coumarin possessed protective and anti-carcinogenic effect against aflatoxicosis in rats.
authors.affiliation: Biochemistry Department, Faculty of Agriculture, Cairo University, Giza, Egypt.
authors.forename: Mohamed S
authors.initials: MS
authors.lastname: Abdel-Latif
authors.affiliation: Regional Center For Food and Feed, Agriculture Research Center, Ministry of Agriculture, Giza, Egypt.
authors.forename: Khaled M
authors.initials: KM
authors.lastname: Elmeleigy
authors.affiliation: Regional Center For Food and Feed, Agriculture Research Center, Ministry of Agriculture, Giza, Egypt.
authors.forename: Tahany A A
authors.initials: TAA
authors.lastname: Aly
authors.affiliation: Pathology Department, Faculty of Veterinary Medicine, Cairo University, Giza Square, Giza, 12211, Egypt. Electronic address: Marwakhattab@cu.edu.eg.
authors.forename: Marwa S
authors.initials: MS
authors.lastname: Khattab
authors.affiliation: Regional Center For Food and Feed, Agriculture Research Center, Ministry of Agriculture, Giza, Egypt.
authors.forename: Sara M
authors.initials: SM
authors.lastname: Mohamed
mesh_terms: D016604:Aflatoxin B1 / Q000633:toxicity*; D000821:Animal Feed; D000818:Animals; D002735:Chlorophyllides / Q000494:pharmacology*; D003374:Coumarins / Q000494:pharmacology*; D005506:Food Contamination; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002735:Chlorophyllides; D003374:Coumarins; D016604:Aflatoxin B1; C030123:coumarin; C007020:chlorophyllin
keywords: Aflatoxin; Chlorophyllin; Coumarin; Immunohistochemistry; Placental glutathione-S-transferase