title: Cyclodextrin modified PLLA parietal reinforcement implant with prolonged antibacterial activity.
abstract: The use of textile meshes in hernia repair is widespread in visceral surgery. Though, mesh infection is a complication that may prolong the patient recovery period and consequently presents an impact on public health economy. Such concern can be avoided thanks to a local and extended antibiotic release on the operative site. In recent developments, poly-l-lactic acid (PLLA) has been used in complement of polyethyleneterephthalate (Dacron®) (PET) or polypropylene (PP) yarns in the manufacture of semi-resorbable parietal implants. The goal of the present study consisted in assigning drug reservoir properties and prolonged antibacterial effect to a 100% PLLA knit through its functionalization with a cyclodextrin polymer (polyCD) and activation with ciprofloxacin. The study focused i) on the control of degree of polyCD functionalization of the PLLA support and on its physical and biological characterization by Scanning Electron Microscopy (SEM), Differential Scanning Calorimetry (DSC) and cell viability, ii) on the understanding of drug/meshes interaction using mathematic model and iii) on the correlation between drug release studies in phosphate buffer saline (PBS) and microbiological evaluation of meshes and release medium against E. coli and S. aureus. All above mentioned tests highlighted the contribution of polyCD on the improved performances of the resulting antibacterial implantable material.
  
  
  
  1. We managed for the first time, with well-defined parameters in terms of temperature and time of treatment, to functionalize a bio-absorbable synthetic material to improve drug sorption and drug release properties without affecting its mechanical properties. 2. We analyzed for the first time the degradation of our coating products by mass spectroscopy to show that only citrate and cyclodextrin residues (and glucose units) without any cytotoxicity are formed. 3. We managed to improve the mechanical properties of the PLA with the cyclodextrin polymer to form a composite. The assembly (cyclodextrin polymer and PLLA) remains biodegradable.
authors.affiliation: Univ. Lille, CNRS, INRA, ENSCL UMR8207, UMET - Unité Matériaux et Transformations, F-59000 Lille, France; Cousin Biotech, 59117 Wervicq-Sud, France.
authors.forename: G
authors.initials: G
authors.lastname: Vermet
authors.affiliation: Univ. Lille, CNRS, INRA, ENSCL UMR8207, UMET - Unité Matériaux et Transformations, F-59000 Lille, France.
authors.forename: S
authors.initials: S
authors.lastname: Degoutin
authors.affiliation: Univ. Lille, Inserm, CHU Lille, U1008 - Controlled Drug Delivery Systems and Biomaterials, F-59000 Lille, France.
authors.forename: F
authors.initials: F
authors.lastname: Chai
authors.affiliation: Univ. Lille, Inserm, CHU Lille, U1008 - Controlled Drug Delivery Systems and Biomaterials, F-59000 Lille, France.
authors.forename: M
authors.initials: M
authors.lastname: Maton
authors.affiliation: Univ. Lille, Inserm, CHU Lille, U1008 - Controlled Drug Delivery Systems and Biomaterials, F-59000 Lille, France.
authors.forename: C
authors.initials: C
authors.lastname: Flores
authors.affiliation: Univ. Lille, Inserm, CHU Lille, U995-LIRIC - Lille Inflammation Research International Center, F-59000 Lille, France.
authors.forename: C
authors.initials: C
authors.lastname: Neut
authors.affiliation: Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV) - EA 4492, Université du Littoral Côte d'Opale, 145 Avenue Maurice Schumann, MREI 1, 59140 Dunkerque, France.
authors.forename: P E
authors.initials: PE
authors.lastname: Danjou
authors.affiliation: Univ. Lille, CNRS, INRA, ENSCL UMR8207, UMET - Unité Matériaux et Transformations, F-59000 Lille, France.
authors.forename: B
authors.initials: B
authors.lastname: Martel
authors.affiliation: Univ. Lille, Inserm, CHU Lille, U1008 - Controlled Drug Delivery Systems and Biomaterials, F-59000 Lille, France. Electronic address: nicolas.blanchemain@univ-lille2.fr.
authors.forename: N
authors.initials: N
authors.lastname: Blanchemain
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D001672:Biocompatible Materials / Q000737:chemistry; D002470:Cell Survival; D002482:Cellulose / Q000737:chemistry*; D003505:Cyclodextrins / Q000737:chemistry*; D016503:Drug Delivery Systems; D004926:Escherichia coli / Q000187:drug effects; D059685:Herniorrhaphy / Q000009:adverse effects / Q000379:methods; D006801:Humans; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D041681:NIH 3T3 Cells; D011091:Polyesters / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects; D013526:Surgical Mesh / Q000009:adverse effects; D013530:Surgical Wound Infection / Q000517:prevention & control; D013784:Textiles / Q000009:adverse effects
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D003505:Cyclodextrins; D011091:Polyesters; C041953:cyclodextrin polymer; C033616:poly(lactide); D002482:Cellulose
keywords: Antibacterial activity; Ciprofloxacin; Cyclodextrin; Cytocompatibility; Degradation study; Drug delivery system; Mechanical properties; Polylactic acid; Visceral mesh