title: Transcriptome Analyses Reveal Candidate Genes Potentially Involved in Al Stress Response in Alfalfa.
abstract: Alfalfa is the most extensively cultivated forage legume, yet most alfalfa cultivars are not aluminum tolerant, and the molecular mechanisms underlying alfalfa responses to Al stress are largely unknown. In this study, we aimed to understand how alfalfa responds to Al stress by identifying and analyzing Al-stress-responsive genes in alfalfa roots at the whole-genome scale. The transcriptome changes in alfalfa roots under Al stress for 4, 8, or 24 h were analyzed using Illumina high-throughput sequencing platforms. A total of 2464 differentially expressed genes (DEGs) were identified, and most were up-regulated at early (4 h) and/or late (24 h) Al exposure time points rather than at the middle exposure time point (8 h). Metabolic pathway enrichment analysis demonstrated that the DEGs involved in ribosome, protein biosynthesis, and process, the citrate cycle, membrane transport, and hormonal regulation were preferentially enriched and regulated. Biosynthesis inhibition and signal transduction downstream of auxin- and ethylene-mediated signals occur during alfalfa responses to root growth inhibition. The internal Al detoxification mechanisms play important roles in alfalfa roots under Al stress. These findings provide valuable information for identifying and characterizing important components in the Al signaling network in alfalfa and enhance understanding of the molecular mechanisms underlying alfalfa responses to Al stress.
authors.affiliation: State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University Lanzhou, China.
authors.forename: Wenxian
authors.initials: W
authors.lastname: Liu
authors.affiliation: Key Laboratory of Mineral Resources in Western China (Gansu Province), School of Earth Sciences, Lanzhou University Lanzhou, China.
authors.forename: Conghui
authors.initials: C
authors.lastname: Xiong
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University Lanzhou, China.
authors.forename: Longfeng
authors.initials: L
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University Lanzhou, China.
authors.forename: Zhengshe
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University Lanzhou, China.
authors.forename: Lichao
authors.initials: L
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University Lanzhou, China.
authors.forename: Yanrong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University Lanzhou, China.
authors.forename: Yajie
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University Lanzhou, China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Liu
keywords: RNA-Seq; alfalfa; aluminum stress; differentially expressed genes; internal detoxification mechanism; transcriptome