title: Chitosan oligosaccharide inhibits EGF-induced cell growth possibly through blockade of epidermal growth factor receptor/mitogen-activated protein kinase pathway.
abstract: Chitosan oligosaccharide (COS) has been shown to regulate various cellular and biological functions. Epidermal growth factor (EGF) plays a significant role in tumorigenesis and invasiveness of some cancers. The aim of this study was to evaluate the effects of COS on EGF-induced cell growth and the further mechanisms. The results demonstrated that COS could inhibit EGF-induced epithelial GE11 cells proliferation in a dose dependent manner. In addition, EGF stimulated the epithelial cells to undergo morphological alteration, exhibiting mesenchymal cells higher metastatic and invasive potential, however, COS could partly suppress aforementioned morphological change. Signal transduction studies indicated COS repressed epidermal growth factor receptor (EGFR) phosphorylation and mitogen-activated protein kinase (MAPK) activation, but not Grb2, Ras, and Raf. Taken together, chitosan oligosaccharide inhibited EGF-induced cell growth and migration through blockade of the EGFR/MAPK signal transduction pathway.
authors.affiliation: College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China. Electronic address: qingsongxu2003@163.com.
authors.forename: Qingsong
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Dalian Elite Analytical Instruments Company Limited, Dalian 116023, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Wang
authors.affiliation: College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Yang
authors.affiliation: Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Du
authors.affiliation: College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
authors.forename: Linsheng
authors.initials: L
authors.lastname: Song
mesh_terms: D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology*; D004815:Epidermal Growth Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D066246:ErbB Receptors / Q000378:metabolism*; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D009844:Oligosaccharides / Q000737:chemistry*; D010766:Phosphorylation / Q000187:drug effects
chemical_list: D009844:Oligosaccharides; D004815:Epidermal Growth Factor; D048271:Chitosan; D066246:ErbB Receptors; D020928:Mitogen-Activated Protein Kinases
keywords: Cell growth; Chitosan oligosaccharide; Epidermal growth factor