title: Knockdown of linc-OIP5 inhibits proliferation and migration of glioma cells through down-regulation of YAP-NOTCH signaling pathway.
abstract: Long intergenic noncoding RNAs (lincRNAs) play important roles in regulating the biological functions and underlying molecular mechanisms of glioma. Here, we investigated the expression level and biological function of linc-OIP5 in glioma. In the current study, we used quantitative real-time polymerase chain reaction (qRT-PCR) to determine the expression of linc-OIP5 in glioma tissues and in adjacent normal tissues. Level of linc-OIP5 was up-regulated in glioma tissues and significantly correlated with the advanced tumor stage (III/IV). Subsequently, the efficacy of knockdown of linc-OIP5 by linc-OIP5-small interfering RNA (siRNA) was evaluated in vitro, and we found that knockdown of linc-OIP5 can inhibit glioma cells proliferation, migration in vitro and tumor formation in vivo. Further mechanistic studies revealed the effect of linc-OIP5 knockdown on glioma cell phenotype at least partially through down-regulation of YAP and inhibition of Notch signaling pathway activity. Thus, our study provides evidence that linc-OIP5 is a potential therapeutic target and novel molecular biomarker for glioma.
authors.affiliation: Department of Neurosurgery, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China. Electronic address: hugw0625@163.com.
authors.forename: Guo-Wen
authors.initials: GW
authors.lastname: Hu
authors.affiliation: Department of Neurosurgery, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China. Electronic address: doctorwundefy@aliyun.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Neurosurgery, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China. Electronic address: chenmingjunndxb@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Kuang
authors.affiliation: Department of Anesthesiology, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China. Electronic address: chenyongdaniel@126.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China. Electronic address: doctorzxgndefy@126.com.
authors.forename: Xin-Gen
authors.initials: XG
authors.lastname: Zhu
authors.affiliation: Department of Neurosurgery, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China. Electronic address: chiefGHndefy@aliyun.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Guo
authors.affiliation: Department of Anesthesiology, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China. Electronic address: langhaili1989@163.com.
authors.forename: Hai-Li
authors.initials: HL
authors.lastname: Lang
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D016543:Central Nervous System Neoplasms / Q000235:genetics* / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics; D015536:Down-Regulation; D005260:Female; D055785:Gene Knockdown Techniques; D005910:Glioma / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D012189:Retrospective Studies; D015398:Signal Transduction
chemical_list: D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; C517146:OIP5 protein, human; D062085:RNA, Long Noncoding; C000615645:long noncoding RNA OIP5, human
keywords: Glioma; Linc-OIP5; Migration; Proliferation; Signaling pathway