title: Determination of Alkaloid Contents in Various Tissues of Coptis Chinensis Franch. by Reversed Phase-High Performance Liquid Chromatography and Ultraviolet Spectrophotometry.
abstract: A simple and intuitive method for optimizing the chemical constituents of Coptis Chinensis Franch. is important to assess its quality and clinical efficacy. An high performance liquid chromatography and ultraviolet spectrophotometry method was developed for the determination of berberine hydrochloride, palmatine chloride, jatrorrhizine hydrochloride, epiberberine, coptisine, columbamine and magnoflorine in various tissues (i.e., phloem, xylem and medulla) and rizhome of C. Chinensis Franch. The transection of rhizome from outside-in includes cork layer, cortex, phloem, cambium, xylem and medulla. Cork layer consists of dead cells, and therefore is not of any research significance. Cortex, phloem and cambium were almost impossible to separate, therefore they were studied as a whole in our experiments. They were collectively referred to as "phloem". The analytes were separated on a Gemini-NX C18 (250 mm × 4.6 mm, 5 μm) reversed phase column using a gradient elution of acetonitrile-0.03 mol/L ammonium acetate solution (containing 0.1% triethylamine and 0.6% ammonium hydroxide) as the mobile phase at a flow rate of 1.0 mL/min and UV detection at 270 nm. The method allowing the simultaneous quantification of seven major active constituents was optimized and validated for linearity, precision, accuracy, limits of detection (LOD) and quantification. The LOD ranged from 0.102 to 0.651 mg/mL (r ≥ 0.9993). Accuracy, precision and recovery were all within the required limits. The average recovery was between 100.14% and 102.75% and the relative standard deviations were <3.34%. At the same time, the absorbance was determined by ultraviolet spectrophotometry at 345 nm wavelength. Based on contents of the seven constituents and clustering result, this investigation suggests that there are significant differences in the distribution of seven alkaloids in the tissues examined. Furthermore, the total alkaloid content in xylem is relatively lower than that in phloem, medulla and rhizome.
authors.affiliation: School of Pharmacy, Hubei University of Chinese Medicine, Hongshan District, Hubei Province, Wuhan 430065, P.R. China.|Collaborative Innovation Center of Traditional Chinese Medicine of New Products for Geriatrics Hubei Province, Huangjia Lake West Road on the 1st, Hubei Province, Wuhan 430065, P.R. China.|Key Laboratory of Resource Science and Chemistry in Chinese Medicine Hubei Province, Huangjia Lake West Road on the 1st, Hubei Province, Wuhan 430061, P.R. China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: School of Pharmacy, Hubei University of Chinese Medicine, Hongshan District, Hubei Province, Wuhan 430065, P.R. China.
authors.forename: Jingling
authors.initials: J
authors.lastname: Peng
authors.affiliation: School of Pharmacy, Hubei University of Chinese Medicine, Hongshan District, Hubei Province, Wuhan 430065, P.R. China.
authors.forename: Fangping
authors.initials: F
authors.lastname: Li
authors.affiliation: School of Pharmacy, Hubei University of Chinese Medicine, Hongshan District, Hubei Province, Wuhan 430065, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: School of Pharmacy, Hubei University of Chinese Medicine, Hongshan District, Hubei Province, Wuhan 430065, P.R. China.
authors.forename: Meng
authors.initials: M
authors.lastname: Deng
authors.affiliation: School of Pharmacy, Hubei University of Chinese Medicine, Hongshan District, Hubei Province, Wuhan 430065, P.R. China.|Collaborative Innovation Center of Traditional Chinese Medicine of New Products for Geriatrics Hubei Province, Huangjia Lake West Road on the 1st, Hubei Province, Wuhan 430065, P.R. China.|Key Laboratory of Resource Science and Chemistry in Chinese Medicine Hubei Province, Huangjia Lake West Road on the 1st, Hubei Province, Wuhan 430061, P.R. China.
authors.forename: Hezhen
authors.initials: H
authors.lastname: Wu
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D039323:Coptis / Q000737:chemistry*; D057230:Limit of Detection; D016014:Linear Models; D018514:Plant Structures / Q000737:chemistry*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D000470:Alkaloids