title: Chitosan-Recombinamer Layer-by-Layer Coatings for Multifunctional Implants.
abstract: The main clinical problems for dental implants are (1) formation of biofilm around the implant-a condition known as peri-implantitis and (2) inadequate bone formation around the implant-lack of osseointegration. Therefore, developing an implant to overcome these problems is of significant interest to the dental community. Chitosan has been reported to have good biocompatibility and anti-bacterial activity. An osseo-inductive recombinant elastin-like biopolymer (P-HAP), that contains a peptide derived from the protein statherin, has been reported to induce biomineralization and osteoblast differentiation. In this study, chitosan/P-HAP bi-layers were built on a titanium surface using a layer-by-layer (LbL) assembly technique. The difference in the water contact angle between consecutive layers, the representative peaks in diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS), X-ray photoelectron spectroscopy (XPS), and the changes in the topography between surfaces with a different number of bi-layers observed using atomic force microscopy (AFM), all indicated the successful establishment of chitosan/P-HAP LbL assembly on the titanium surface. The LbL-modified surfaces showed increased biomineralization, an appropriate mouse pre-osteoblastic cell response, and significant anti-bacterial activity against Streptococcus gordonii, a primary colonizer of tissues in the oral environment.
authors.affiliation: Department of Bioproducts and Biosystems Engineering, University of Minnesota, St. Paul, MN 55108, USA. gprasaad88@gmail.com.
authors.forename: Jeevan Prasaad
authors.initials: JP
authors.lastname: Govindharajulu
authors.affiliation: Department of Restorative Sciences, School of Dentistry, University of Minnesota, Minneapolis, MN 55455, USA. chenx838@umn.edu.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Restorative Sciences, School of Dentistry, University of Minnesota, Minneapolis, MN 55455, USA. lixx1191@umn.edu.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Li
authors.affiliation: BIOFORGE Group, University of Valladolid, Valladolid 4701, Spain. roca@bioforge.uva.es.
authors.forename: Jose Carlos
authors.initials: JC
authors.lastname: Rodriguez-Cabello
authors.affiliation: Department of Bioproducts and Biosystems Engineering, University of Minnesota, St. Paul, MN 55108, USA. bhatt002@umn.edu.
authors.forename: Mrinal
authors.initials: M
authors.lastname: Battacharya
authors.affiliation: Department of Restorative Sciences, School of Dentistry, University of Minnesota, Minneapolis, MN 55455, USA. apari003@umn.edu.
authors.forename: Conrado
authors.initials: C
authors.lastname: Aparicio
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry; D002448:Cell Adhesion; D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D015921:Dental Implants; D018625:Microscopy, Atomic Force; D016348:Osseointegration; D010006:Osteoblasts / Q000378:metabolism; D056951:Photoelectron Spectroscopy; D019736:Prostheses and Implants; D011506:Proteins / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D011506:Proteins; D048271:Chitosan; D014025:Titanium
keywords: chitosan; elastin-like recombinamers; implant; layer-by-layer; titanium