title: Histaminergic H1 and H2 Receptors Mediate the Effects of Propofol on the Noradrenalin-Inhibited Neurons in Rat Ventrolateral Preoptic Nucleus.
abstract: The ventrolateral preoptic nucleus is a sleep-promoting nucleus located in the basal forebrain. A commonly used intravenous anesthetic, propofol, had been reported to induce sleep spindles and augment the firing rate of neurons in ventrolateral preoptic nucleus, but the underlining mechanism is yet to be known. By using patch clamp recording on neuron in acute brain slice, present study tested if histaminergic H1 and H2 receptors play a role in the effect of propofol on the noradrenalin-inhibited neurons in ventrolateral preoptic nucleus. We found that the firing rate of noradrenalin-inhibited neurons were significantly augmented by propofol; the frequency of inhibitory postsynaptic currents of noradrenalin-inhibited neuron were evidently attenuated by propofol; such inhibition effect was suppressed by histamine; and both triprolidine (antagonist for H1 histamine receptor) and ranitidine (antagonist for H2 histamine receptor) were able to increase the inhibition rate of propofol in presence of histamine. Present study demonstrated that propofol-induced inhibition of inhibitory postsynaptic currents on noradrenalin-inhibited neurons were mediated by histaminergic H1 and H2 receptors.
authors.affiliation: Department of Anesthesiology, Affiliated Hospital of Zunyi Medical College, Zunyi, 563000, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Guizhou Key Laboratory of Anesthesia and Organ Protection, Zunyi Medical University, Zunyi, 563000, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, Affiliated Hospital of Zunyi Medical College, Zunyi, 563000, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Qian
authors.affiliation: Guizhou Key Laboratory of Anesthesia and Organ Protection, Zunyi Medical University, Zunyi, 563000, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Guizhou Key Laboratory of Anesthesia and Organ Protection, Zunyi Medical University, Zunyi, 563000, China. dllyutian@163.com.|Zunyi Medical College, Dalian road 149, Zunyi, 563000, Guizhou, China. dllyutian@163.com.
authors.forename: Tian
authors.initials: T
authors.lastname: Yu
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000818:Animals; D004305:Dose-Response Relationship, Drug; D006632:Histamine / Q000494:pharmacology; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D006993:Hypnotics and Sedatives / Q000494:pharmacology; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009638:Norepinephrine / Q000494:pharmacology*; D009924:Organ Culture Techniques; D011301:Preoptic Area / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D015742:Propofol / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011969:Receptors, Histamine H1 / Q000502:physiology*; D011970:Receptors, Histamine H2 / Q000502:physiology*
chemical_list: D006634:Histamine H1 Antagonists; D006635:Histamine H2 Antagonists; D006993:Hypnotics and Sedatives; D011969:Receptors, Histamine H1; D011970:Receptors, Histamine H2; D006632:Histamine; D009638:Norepinephrine; D015742:Propofol
keywords: General anesthesia; Inhibitory postsynaptic currents; Propofol; Ventrolateral preoptic nucleus