title: Soy-Leaf Extract Exerts Atheroprotective Effects via Modulation of Krüppel-Like Factor 2 and Adhesion Molecules.
abstract: Soy-leaf extracts exert their cardioprotective effects by inducing endothelium-dependent vasodilation in the arteries, and they favorably modulate the serum lipid profile. In this study, we investigated the atheroprotective effects of an ethanol extract of soy leaf (ESL) in human umbilical vein endothelial cells (HUVECs) and high-cholesterol diet (HCD)-fed low-density lipoprotein receptor deficient (LDLR-/-) mice. ESL induced the expression of Krüppel-like factor 2 (KLF2), an endothelial transcription factor, and endothelial nitric oxide synthase (eNOS), and suppressed the expression of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) through moderate inflammatory signal activation, not only in tumor necrosis factor-α (TNF-α)-stimulated HUVECs but also in 7-ketocholesterol (7-KC)-stimulated HUVECs. ESL supplementation reduced aortic lesion formation in Western diet-fed LDLR-/- mice by 46% (p < 0.01) compared to the HCD group. ESL also markedly decreased the aortic expression levels of VCAM-1, ICAM-1, monocyte chemotactic protein-1 (MCP-1), TNF-α, IL-6, IL-1β, matrix metallopeptidase 9 (MMP-9), and fractalkine, while the expression of KLF2 was significantly increased. These results suggest that ESL supplementation has potential for preventing HCD-induced atherosclerosis effectively.
authors.affiliation: Division of Life Science, Daejeon University, Daejeon 300-716, Korea. malius@dju.kr.
authors.forename: Jong-Min
authors.initials: JM
authors.lastname: Han
authors.affiliation: Industrial Bio-materials Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea. leehua@kribb.re.kr.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.affiliation: Industrial Bio-materials Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea. chomh21@gmail.com.
authors.forename: Moon-Hee
authors.initials: MH
authors.lastname: Cho
authors.affiliation: Industrial Bio-materials Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea. micro340@hanmail.net.
authors.forename: Seung-Hwa
authors.initials: SH
authors.lastname: Baek
authors.affiliation: Laboratory Animal Resource Center, KRIBB, Daejeon 305-806, Korea. chullee@kribb.re.kr.
authors.forename: Chul-Ho
authors.initials: CH
authors.lastname: Lee
authors.affiliation: Industrial Bio-materials Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea. hypark@kribb.re.kr.
authors.forename: Ho-Yong
authors.initials: HY
authors.lastname: Park
authors.affiliation: Industrial Bio-materials Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea. tsjeong@kribb.re.kr.|Department of Biomolecular Science, Korea University of Science and Technology, KRIBB, Daejeon 305-806, Korea. tsjeong@kribb.re.kr.
authors.forename: Tae-Sook
authors.initials: TS
authors.lastname: Jeong
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism / Q000473:pathology; D050197:Atherosclerosis / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D020011:Protective Agents / Q000494:pharmacology*; D011973:Receptors, LDL / Q000172:deficiency; D013025:Glycine max / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism / Q000494:pharmacology
chemical_list: D015815:Cell Adhesion Molecules; C096161:KLF2 protein, human; D051741:Kruppel-Like Transcription Factors; D010936:Plant Extracts; D020011:Protective Agents; D011973:Receptors, LDL; D014409:Tumor Necrosis Factor-alpha; D052250:Nitric Oxide Synthase Type III
keywords: KLF2; adhesion molecule; atherosclerosis; inflammation; soy leaf