title: Genome-wide characterization of lncRNAs in acute myeloid leukemia.
abstract: Long noncoding RNAs (lncRNAs) are a large family of noncoding RNAs that play a critical role in various normal bioprocesses as well as tumorigenesis. However, the expression patterns and biological functions of lncRNAs in acute leukemia have not been well studied. Here, we performed transcriptome-wide lncRNA expression profiling of acute myeloid leukemia (AML) patient samples, along with non-leukemia control hematopoietic samples. We found that lncRNAs were differentially expressed in AML samples relative to control samples. Notably, we identified that lncRNAs upregulated in AML (relative to the control samples) are associated with a lower degree of DNA methylation and a higher ratio of being bound by transcription factors such as SP1, STAT4, ATF-2 and ELK-1 compared with those downregulated in AML. Moreover, an enrichment of H3K4me3 and a depletion of H3K27me3 were observed in upregulated lncRNAs in AML. Expression patterns of three types of lncRNAs (antisense, enhancer and intergenic lncRNAs) have previously been characterized. Of the identified lncRNAs, we found that high expression level lncRNA LOC285758 is associated with the poor prognosis in AML patients. Furthermore, we found that LOC285758 regulates proliferation of AML cell lines by enhancing the expression of HDAC2, a key factor in carcinogenesis. Collectively, our study depicts a landscape of important lncRNAs in AML and provides novel potential therapeutic targets and prognostic markers for AML treatment.
authors.affiliation: School of Basic Medical Sciences, Wuhan University, Wuhan, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Lei
authors.affiliation: School of Basic Medical Sciences, Wuhan University, Wuhan, China.
authors.forename: Siyu
authors.initials: S
authors.lastname: Xia
authors.affiliation: School of Basic Medical Sciences, Wuhan University, Wuhan, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.affiliation: Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Li
authors.affiliation: International School of Software, Wuhan University, Wuhan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Feng
authors.affiliation: School of Basic Medical Sciences, Wuhan University, Wuhan, China.
authors.forename: Yaqi
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: School of Basic Medical Sciences, Wuhan University, Wuhan, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.affiliation: School of Basic Medical Sciences, Wuhan University, Wuhan, China.
authors.forename: Linjian
authors.initials: L
authors.lastname: Xia
authors.affiliation: Physician in Eastern Hepatobiliary Hospital, Hospital of Second Military Medical University, Wuhan, Hubei, China.
authors.forename: Lieping
authors.initials: L
authors.lastname: Guo
authors.affiliation: Zhongnan hospital of Wuhan University, Wuhan, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Chen
authors.affiliation: Changhai Hospital, Second Military Medical University, Shanghai, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Department of Medicine and Cancer Research Center, University of Illinois at Chicago, Chicago, USA.
authors.forename: Ke
authors.initials: K
authors.lastname: Chen
authors.affiliation: School of Basic Medical Sciences, Wuhan University, Wuhan, China.
authors.forename: Hanyang
authors.initials: H
authors.lastname: Hu
authors.affiliation: School of Basic Medical Sciences, Wuhan University, Wuhan, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.affiliation: School of Basic Medical Sciences, Wuhan University, Wuhan, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Zhong
authors.affiliation: Department of Medicine and Cancer Research Center, University of Illinois at Chicago, Chicago, USA.
authors.forename: Nupur
authors.initials: N
authors.lastname: Mittal
authors.affiliation: School of Basic Medical Sciences, Wuhan University, Wuhan, China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of Medicine and Cancer Research Center, University of Illinois at Chicago, Chicago, USA.
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Qian
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston McGovern Medical School, Houston, TX, USA.
authors.forename: Leng
authors.initials: L
authors.lastname: Han
authors.affiliation: School of Basic Medical Sciences, Wuhan University, Wuhan, China.
authors.forename: Chunjiang
authors.initials: C
authors.lastname: He
mesh_terms: D016022:Case-Control Studies; D015972:Gene Expression Regulation, Neoplastic; D056464:Histone Deacetylase 2 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000235:genetics*; D059467:Transcriptome; D014407:Tumor Cells, Cultured
chemical_list: D062085:RNA, Long Noncoding; C533423:HDAC2 protein, human; D056464:Histone Deacetylase 2