title: CCCTC-Binding Factor Transcriptionally Targets Wdr5 to Mediate Somatic Cell Reprogramming.
abstract: It was previously reported that WD repeat domain 5 (Wdr5), a core member of the mammalian Trithorax complex, is a key regulator for the maintenance of embryonic stem (ES) cell pluripotency and somatic cell reprogramming. However, it remains unclear whether other factors are also involved in this process. Here, we show that CTCF is an upstream regulator of Wdr5: It physically associates with Wdr5 and further transcriptionally controls its expression by directly targeting the Wdr5 gene promoter. As a downstream effector, overexpression of Wdr5 can rescue ES cells from growth defects and decreased formation of induced pluripotent stem cells caused by CTCF knockdown. Our results reveal the functional relevance of CTCF and Wdr5, and they connect them to the re-establishment of pluripotency.
authors.affiliation: 1 Faculty of Life Science and Technology, Kunming University of Science and Technology , Kunming, China .|2 Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology , Kunming, China .
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: 2 Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology , Kunming, China .
authors.forename: Jinghua
authors.initials: J
authors.lastname: Han
authors.affiliation: 3 Huanghe College of Science and Technology , Zhengzhou City, China .
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: 4 Department of Oncology, The First Affiliated Hospital of Kunming Medical University , Kunming, China .
authors.forename: Ying
authors.initials: Y
authors.lastname: Jing
authors.affiliation: 4 Department of Oncology, The First Affiliated Hospital of Kunming Medical University , Kunming, China .
authors.forename: Zhu
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: 1 Faculty of Life Science and Technology, Kunming University of Science and Technology , Kunming, China .
authors.forename: Dawei
authors.initials: D
authors.lastname: Hui
authors.affiliation: 1 Faculty of Life Science and Technology, Kunming University of Science and Technology , Kunming, China .
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Wang
authors.affiliation: 1 Faculty of Life Science and Technology, Kunming University of Science and Technology , Kunming, China .
authors.forename: Yangzi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: 1 Faculty of Life Science and Technology, Kunming University of Science and Technology , Kunming, China .
authors.forename: Yang
authors.initials: Y
authors.lastname: Dong
authors.affiliation: 1 Faculty of Life Science and Technology, Kunming University of Science and Technology , Kunming, China .|2 Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology , Kunming, China .
authors.forename: Tao
authors.initials: T
authors.lastname: Tan
mesh_terms: D000818:Animals; D001665:Binding Sites; D000076246:CCCTC-Binding Factor / Q000235:genetics / Q000378:metabolism*; D065150:Cellular Reprogramming; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D000076246:CCCTC-Binding Factor; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; C443118:Wdr5 protein, mouse
keywords: CTCF; Wdr5; pluripotency; reprogramming; stem cells