title: Superoxide Generation and Its Involvement in the Growth ofMycobacterium smegmatis.
abstract: Superoxide generation is inevitable in aerobic organisms, most of which have developed mechanisms to detoxify superoxides. However, its significance has not been clearly understood in mycobacteria. This study demonstrates that NADH oxidase is the major source of superoxide inMycobacterium smegmatisand elucidates the involvement of superoxide inM. smegmatisgrowth. The maximum inhibition of superoxide generation was observed in the presence of diphenyleneiodonium chloride (DPI), an NADH oxidase inhibitor, compared to other standard inhibitors. After incubation for 24 h, the number of colony forming units (CFUs) was reduced by 6.8 log10compared to the untreated culture. The inhibitory effect of DPI onM. smegmatiswas reversed when the same culture was supplemented with menadione and pyrogallol, which are superoxide generators. Thus, this study reports the source of superoxide generation and its involvement in the growth ofM. smegmatis.
authors.affiliation: Combi Chem-Bio Resource Center, Organic Chemistry Division, CSIR-National Chemical Laboratory Pune, India.
authors.forename: Amar M
authors.initials: AM
authors.lastname: Yeware
authors.affiliation: Combi Chem-Bio Resource Center, Organic Chemistry Division, CSIR-National Chemical Laboratory Pune, India.
authors.forename: Ketaki D
authors.initials: KD
authors.lastname: Shurpali
authors.affiliation: Combi Chem-Bio Resource Center, Organic Chemistry Division, CSIR-National Chemical Laboratory Pune, India.
authors.forename: Meghana C
authors.initials: MC
authors.lastname: Athalye
authors.affiliation: Combi Chem-Bio Resource Center, Organic Chemistry Division, CSIR-National Chemical Laboratory Pune, India.
authors.forename: Dhiman
authors.initials: D
authors.lastname: Sarkar
keywords: Mycobacterium smegmatis; NADH oxidase; Reactive oxygen species; diphenyleneiodonium chloride; mycobacteria growth; superoxide