title: Multiresponse optimization of a UPLC method for the simultaneous determination of tryptophan and 15 tryptophan-derived compounds using a Box-Behnken design with a desirability function.
abstract: A Box-Behnken design was used in conjunction with multiresponse optimization based on the desirability function to carry out the simultaneous separation of tryptophan and 15 derivatives by Ultra Performance Liquid Chromatography. The gradient composition of the mobile phase and the flow rate were optimized with respect to the resolution of severely overlapping chromatographic peaks and the total run time. Two different stationary phases were evaluated (hybrid silica and a solid-core-based C18column). The methods were validated and a suitable sensitivity was found for all compounds in the concentration range 1-100μgL-1(R2>0.999). High levels of repeatability and intermediate precision (CV less than 0.25% and 1.7% on average for the retention time and the signal area, respectively) were obtained. The new method was applied to the determination tryptophan and its derivatives in black pigmented glutinous and non-glutinous rice grain samples.
authors.affiliation: Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Gadjah Mada University, Jalan Flora, Bulaksumur, 55281 Yogyakarta, Indonesia; Department of Analytical Chemistry, Faculty of Sciences, Instituto de Investigación Vitivinícola y Agroalimentaria (IVAGRO), University of Cadiz, Campus de Excelencia Internacional Agroalimentario (CeiA3), Campus del Rio San Pedro, 11510, Puerto Real, Cádiz, Spain.
authors.forename: Widiastuti
authors.initials: W
authors.lastname: Setyaningsih
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, Instituto de Investigación Vitivinícola y Agroalimentaria (IVAGRO), University of Cadiz, Campus de Excelencia Internacional Agroalimentario (CeiA3), Campus del Rio San Pedro, 11510, Puerto Real, Cádiz, Spain.
authors.forename: Irfan E
authors.initials: IE
authors.lastname: Saputro
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, Instituto de Investigación Vitivinícola y Agroalimentaria (IVAGRO), University of Cadiz, Campus de Excelencia Internacional Agroalimentario (CeiA3), Campus del Rio San Pedro, 11510, Puerto Real, Cádiz, Spain.
authors.forename: Ceferino A
authors.initials: CA
authors.lastname: Carrera
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, Instituto de Investigación Vitivinícola y Agroalimentaria (IVAGRO), University of Cadiz, Campus de Excelencia Internacional Agroalimentario (CeiA3), Campus del Rio San Pedro, 11510, Puerto Real, Cádiz, Spain. Electronic address: miguel.palma@uca.es.
authors.forename: Miguel
authors.initials: M
authors.lastname: Palma
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, Instituto de Investigación Vitivinícola y Agroalimentaria (IVAGRO), University of Cadiz, Campus de Excelencia Internacional Agroalimentario (CeiA3), Campus del Rio San Pedro, 11510, Puerto Real, Cádiz, Spain.
authors.forename: Carmelo G
authors.initials: CG
authors.lastname: Barroso
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D014364:Tryptophan
keywords: 1-Methyl-l-tryptophan (PubChem CID: 676159); 3-Indolepropionic acid (PubChem CID: 3744); 5-Hydroxy-l-tryptophan (PubChem CID: 439280); 5-Methoxy-dl-tryptophan (PubChem CID: 119802); 5-Methoxytryptamine (PubChem CID: 6198); Chromatographic separation; Design of experiments; Indole-3-acetonitrile (PubChem CID: 351795); Indole-3-butyric acid (PubChem CID: 273800); Indole-3-carboxylic acid (PubChem CID: 69867); Kynurenine (PubChem CID: 846); Melatonin (PubChem CID: 896); N-Acetyl-5-hydroxytryptamine (PubChem CID: 903); N-acetyl-l-tryptophan (PubChem CID: 700653); Serotonin (PubChem CID: 5202); Tryptamine (PubChem CID: 1150); Tryptophan; Tryptophan derivatives; l-Tryptophan (PubChem CID: 6305)