title: Lepteridine as a unique fluorescent marker for the authentication of manuka honey.
abstract: The recent discovery of two unique manuka marker fluorescence wavelengths (MM1 and MM2) potentially offers a rapid and cost-effective approach for manuka honey authentication using spectroscopy. The fluorophore responsible for the MM1 marker has been identified as leptosperin. We investigated whether lepteridine may be responsible for the MM2 fluorescence. We quantified the lepteridine in manuka honey and manuka nectar, which ranged between 5-52mg/kg and 80-205mg/kg, respectively. Notably, the fluorescent spectrum of synthetic lepteridine matched the MM2 fluorescence signature. Fluorescence quenching was observed in the honey matrix but otherwise, lepteridine was stable over prolonged storage at 37°C. Lepteridine was also found in Australian Leptospermum honeys and nectars. Lepteridine concentration was positively correlated with concentrations of the MM1 fluorescence marker leptosperin in honeys. These findings identify lepteridine as the principle compound responsible for MM2 fluorescence, and support the utility as a marker compound for manuka honey authentication.
authors.affiliation: School of Biological Sciences and Institute for Innovation in Biotechnology, University of Auckland, PB92019 Auckland, New Zealand.
authors.forename: Bin
authors.initials: B
authors.lastname: Lin
authors.affiliation: School of Biological Sciences and Institute for Innovation in Biotechnology, University of Auckland, PB92019 Auckland, New Zealand.
authors.forename: Kerry M
authors.initials: KM
authors.lastname: Loomes
authors.affiliation: School of Biological Sciences and Institute for Innovation in Biotechnology, University of Auckland, PB92019 Auckland, New Zealand; Comvita NZ Limited, Wilson South Road, Paengaroa, PB1, Te Puke, New Zealand.
authors.forename: Gordana
authors.initials: G
authors.lastname: Prijic
authors.affiliation: Comvita NZ Limited, Wilson South Road, Paengaroa, PB1, Te Puke, New Zealand.
authors.forename: Ralf
authors.initials: R
authors.lastname: Schlothauer
authors.affiliation: School of Biological Sciences and Institute for Innovation in Biotechnology, University of Auckland, PB92019 Auckland, New Zealand; Comvita NZ Limited, Wilson South Road, Paengaroa, PB1, Te Puke, New Zealand. Electronic address: jonathan.stephens@comvita.com.
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Stephens
mesh_terms: D015415:Biomarkers; D006722:Honey / Q000032:analysis*; D011621:Pteridines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: C000617211:3,6,7-trimethyllumazine; D015415:Biomarkers; D011621:Pteridines
keywords: Fluorescence spectroscopy; Honey authentication; Lepteridine; Leptospermum honey; Manuka honey