title: TiO2nanowire arrays modified with a simultaneous "etching, doping and deposition" technique for ultrasensitive amperometric immunosensing.
abstract: In this work, an ultrasensitive immunosensing scaffold was structured with TiO2nanowire (TiNW) arrays modified with molybdenum (Mo) and MoS2flakes by a triplex "etching, doping and deposition" technique. The triply modification of TiNW arrays improved their electron transfer, and the decoration of MoS2flakes on TiNW arrays increased both the conductivity and the specific surface area of TiNW. Accordingly, the triply modified TiNW arrays provided a biocompatible microenviroment for the biomolecules and high specific surface area to load big amount of biomolecules. The immunosensor was prepared by immobilizing capture antibody on the scaffold surface with double amino-reactive crosslinker, and the tracing labels were prepared by immobilizing signal antibody and horseradish peroxidase molecules on cylinder-shaped TiO2nanorods. After sandwich-type immunoreaction, the tracing labels were quantitatively captured on the immunosensor surface for the detection of carcinoembryonic antigen as a model analyte. This amperometric method showed a linear range of 0.001 and 150ngmL-1with a detection limit of 0.5pgmL-1. This work provided a promising platform for sensitive amperometric immunosensing of protein biomarkers.
authors.affiliation: Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan Province 475004, PR China. Electronic address: 13781157777@163.com.
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan Province 475004, PR China.
authors.forename: Xiaohe
authors.initials: X
authors.lastname: Huo
authors.affiliation: Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan Province 475004, PR China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Liu
authors.affiliation: Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan Province 475004, PR China.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan Province 475004, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing 210023, PR China. Electronic address: hxju@nju.edu.cn.
authors.forename: Huangxian
authors.initials: H
authors.lastname: Ju
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D002272:Carcinoembryonic Antigen / Q000032:analysis / Q000097:blood*; D055664:Electrochemical Techniques / Q000379:methods; D006801:Humans; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D053770:Nanowires / Q000737:chemistry* / Q000648:ultrastructure; D014025:Titanium / Q000737:chemistry*
chemical_list: D055910:Antibodies, Immobilized; D002272:Carcinoembryonic Antigen; C009495:titanium dioxide; D014025:Titanium
keywords: Amperometry; Carcinoembryonic antigen; Immunosensor; MoS(2); TiO(2) nanorod; TiO(2) nanowire arrays