title: Overexpression of SmeDEF Efflux Pump Decreases Aminoglycoside Resistance in Stenotrophomonas maltophilia.
abstract: The SmeDEF pump ofStenotrophomonas maltophiliais negatively regulated by SmeT. In this study, strains KJΔT (smeTdeletion mutant) and KJT-Dm(mutant with a defective SmeT-binding site) showed increased resistance to chloramphenicol/nalidixic acid/macrolides and susceptibility to aminoglycoside. Overexpression of the SmeDEF pump, in either KJΔT or KJT-Dm, downregulatedsmeYZexpression, which is responsible for the reduced aminoglycoside resistance. Furthermore, the SmeRySy two-component regulatory system was downregulated in response to SmeDEF overexpression, which supports its involvement in the regulatory circuit.
authors.affiliation: Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Yi-Wei
authors.initials: YW
authors.lastname: Huang
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan.
authors.forename: Cheng-Wen
authors.initials: CW
authors.lastname: Lin
authors.affiliation: Department of Laboratory Medicine, Chang Gung Memorial Hospital Linkou Branch, Taoyuan, Taiwan.|Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Taoyuan, Taiwan.
authors.forename: Hsiao-Chen
authors.initials: HC
authors.lastname: Ning
authors.affiliation: Division of Infectious Diseases, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan.|School of Medicine, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Yi-Tsung
authors.initials: YT
authors.lastname: Lin
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan yichih@mail.cmu.edu.tw tcyang@ym.edu.tw.
authors.forename: Yi-Chih
authors.initials: YC
authors.lastname: Chang
authors.affiliation: Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan yichih@mail.cmu.edu.tw tcyang@ym.edu.tw.
authors.forename: Tsuey-Ching
authors.initials: TC
authors.lastname: Yang
mesh_terms: D000583:Amikacin / Q000494:pharmacology; D000617:Aminoglycosides / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D002701:Chloramphenicol / Q000494:pharmacology; D002939:Ciprofloxacin / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D004917:Erythromycin / Q000494:pharmacology; D005839:Gentamicins / Q000494:pharmacology; D007612:Kanamycin / Q000494:pharmacology; D026901:Membrane Transport Proteins / Q000096:biosynthesis* / Q000235:genetics; D020615:Stenotrophomonas maltophilia / Q000187:drug effects* / Q000235:genetics*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D005839:Gentamicins; D026901:Membrane Transport Proteins; C417265:SmeD protein, Stenotrophomonas maltophilia; C417266:SmeE protein, Stenotrophomonas maltophilia; C417267:SmeF protein, Stenotrophomonas maltophilia; D007612:Kanamycin; D002939:Ciprofloxacin; D004917:Erythromycin; D002701:Chloramphenicol; D000583:Amikacin
keywords: antibiotic resistance; bacteria; efflux pump; two-component regulatory system