title: Extraction of proteins and preliminary characterization of physicochemical properties in Toona sinensis fruit.
abstract: We investigated the extraction of Toona sinensis fruit proteins and preliminarily characterized their physicochemical properties. The results showed that optimal extraction occurred under conditions of pH 10.5, a duration of 40 min, a liquid-to-solid ratio of 25:1, and a temperature of 40°C by an orthogonal design using T. sinensis fruit protein as the index and single factor. The total nitrogen content was 13.8 g/100 g and included 17 different amino acids. The glutamate level was highest at 35.37%, followed by arginine at 15.31%. The isoelectric point of T. sinensis fruit protein was between 6.8 and 10.0 with a typical absorption peak by infrared chromatography. Three protein bands were analyzed using SDS-polyacrylamide gel electrophoresis, with relative molecular weights of 55, 51, and 22 kDa. This study provides a theoretical basis for the comprehensive utilization of T. sinensis fruit by further investigating the biological activity of its proteins.
authors.affiliation: College of Pharmacy, Weifang Medical University, Weifang, People's Republic of China wanzhong_06@163.com.
authors.forename: W-Z
authors.initials: WZ
authors.lastname: Li
authors.affiliation: College of Pharmacy, Weifang Medical University, Weifang, People's Republic of China.
authors.forename: W-N
authors.initials: WN
authors.lastname: Han
authors.affiliation: College of Pharmacy, Weifang Medical University, Weifang, People's Republic of China.
authors.forename: B
authors.initials: B
authors.lastname: Liu
authors.affiliation: College of Pharmacy, Weifang Medical University, Weifang, People's Republic of China.
authors.forename: S-H
authors.initials: SH
authors.lastname: Ding
authors.affiliation: College of Pharmacy, Weifang Medical University, Weifang, People's Republic of China.
authors.forename: X-K
authors.initials: XK
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Weifang Medical University, Weifang, People's Republic of China.
authors.forename: R-S
authors.initials: RS
authors.lastname: Wang
mesh_terms: D005638:Fruit / Q000737:chemistry*; D029582:Meliaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D040901:Proteomics / Q000379:methods
chemical_list: D010936:Plant Extracts; D010940:Plant Proteins