title: Rapid expression of RASD1 is regulated by estrogen receptor-dependent intracellular signaling pathway in the mouse uterus.
abstract: Dexamethasone-induced RAS-related protein 1 (RASD1) is a signaling protein that is involved in various cellular processes. In a previous study, we found that RASD1 expression was down-regulated in the uterine endometrium of repeated implantation failure patients. The study aim was to determine whether RASD1 is expressed in the endometrium of mouse uterus and how it is regulated by steroid hormones during the estrous cycle. In this study, we investigated RASD1 expression and regulation in an ovariectomized female mouse model. Rasd1 mRNA was highly expressed in mouse reproductive tissues, including the uterus. Rasd1 expression was detected exclusively in the endometrial epithelium at the proestrus stage of the estrous cycle. Rasd1 expression in uteri increased with administration of estradiol, but not progesterone. Its expression was rapidly induced within 2 h after E2treatment. Pretreatment with ICI 182,780, an estrogen receptor antagonist, reduced RASD1 protein expression. In addition, we identified that rapid expression of Rasd1 was mediated by the estrogen intracellular signaling including both p38-mitogen-activated protein kinase and the extracellular signal-regulated kinase. These findings suggest that RASD1 acts as a novel signaling molecule and plays an important role in regulating dynamic uterine remodeling during the estrous cycle in the uterus.
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 335 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, 13488, Republic of Korea.
authors.forename: Hye-Ryun
authors.initials: HR
authors.lastname: Kim
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 335 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, 13488, Republic of Korea.
authors.forename: Kil-Sang
authors.initials: KS
authors.lastname: Cho
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 335 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, 13488, Republic of Korea.
authors.forename: Eunhye
authors.initials: E
authors.lastname: Kim
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 335 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, 13488, Republic of Korea.
authors.forename: Ok-Hee
authors.initials: OH
authors.lastname: Lee
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 335 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, 13488, Republic of Korea.
authors.forename: Hyemin
authors.initials: H
authors.lastname: Yoon
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 335 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, 13488, Republic of Korea.
authors.forename: Sangho
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 335 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, 13488, Republic of Korea.
authors.forename: Sohyeon
authors.initials: S
authors.lastname: Moon
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 335 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, 13488, Republic of Korea.
authors.forename: Miseon
authors.initials: M
authors.lastname: Park
authors.affiliation: Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan-si, Chungcheongnam-do, 31116, Republic of Korea.
authors.forename: Kwonho
authors.initials: K
authors.lastname: Hong
authors.affiliation: Department of Pharmacology, College of Pharmacy, CHA University, 335 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, 13488, Republic of Korea.
authors.forename: Younghwa
authors.initials: Y
authors.lastname: Na
authors.affiliation: Fertility Center of CHA Bundang Medical Center, 59 Yatap-ro, Bundang-gu, Seongnam-si, 13496, Republic of Korea.
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Shin
authors.affiliation: Fertility Center of CHA Bundang Medical Center, 59 Yatap-ro, Bundang-gu, Seongnam-si, 13496, Republic of Korea.
authors.forename: Hwang
authors.initials: H
authors.lastname: Kwon
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 335 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, 13488, Republic of Korea.
authors.forename: Haengseok
authors.initials: H
authors.lastname: Song
authors.affiliation: Fertility Center of CHA Bundang Medical Center, 59 Yatap-ro, Bundang-gu, Seongnam-si, 13496, Republic of Korea.
authors.forename: Dong Hee
authors.initials: DH
authors.lastname: Choi
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 335 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, 13488, Republic of Korea. Electronic address: youngsokchoi@cha.ac.kr.
authors.forename: Youngsok
authors.initials: Y
authors.lastname: Choi
mesh_terms: D000818:Animals; D004717:Endometrium / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology; D030762:Estrous Cycle / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D042541:Intracellular Space / Q000378:metabolism*; D051379:Mice; D010052:Ovariectomy; D011374:Progesterone / Q000494:pharmacology; D011960:Receptors, Estrogen / Q000378:metabolism*; D012741:Sexual Maturation / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism; D018631:ras Proteins / Q000378:metabolism*
chemical_list: D011960:Receptors, Estrogen; D011374:Progesterone; D004958:Estradiol; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases; C498511:Rasd1 protein, mouse; D018631:ras Proteins
keywords: Estrogen; Estrogen-receptor; RASD1; Uterus