title: Salmonella effector SopD2 interferes with Rab34 function.
abstract: Many intracellular pathogens have evolved highly specialized mechanisms to isolate themselves from the host cell's innate immune response while still obtaining the necessary nutrients to survive. Salmonella utilizes type 3 secretion systems (T3SSs) to deliver bacterial proteins called effectors, across the encompassing Salmonella Containing vacuole (SCV) membrane, to subvert the host's membrane trafficking pathways and alter other cellular processes. The Salmonella Pathogenicity Island (SPI)-2 effector SopD2 has recently been demonstrated to modulate multiple members of the Rab GTPase family such as Rab7, Rab8, Rab10, and Rab32 (D'Costa et al., , Cell Reports, 12:1508-18; Spano et al., , Cell Host & Microbe, 19:216-26). Here, we demonstrate the additional capacity of SopD2 to bind Rab34 and modulate its function. Our data indicate that depletion of Rab34 delays maturation of the SCV, and consequently, inhibits intracellular Salmonella enterica serotype typhimurium (S. typhimurium) growth. Interestingly, intracellular growth of the S. typhimurium lacking SopD2 was severely impaired in Rab34-depleted cells, suggesting a compounding virulence effect. Overall this study reveals an additional member of the Rab GTPase family, Rab34, that is modulated by SopD2 and provides insight into its role in Salmonella biology.
authors.affiliation: Institute for Molecular Biosciences, University of Queensland, St. Lucia, QLD, 4072, Australia.
authors.forename: Wei Xuan
authors.initials: WX
authors.lastname: Teo
authors.affiliation: Institute for Molecular Biosciences, University of Queensland, St. Lucia, QLD, 4072, Australia.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Institute for Molecular Biosciences, University of Queensland, St. Lucia, QLD, 4072, Australia.
authors.forename: Markus Charles
authors.initials: MC
authors.lastname: Kerr
authors.affiliation: Institute for Molecular Biosciences, University of Queensland, St. Lucia, QLD, 4072, Australia.
authors.forename: Lin
authors.initials: L
authors.lastname: Luo
authors.affiliation: Institute for Molecular Biosciences, University of Queensland, St. Lucia, QLD, 4072, Australia.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Institute for Molecular Biosciences, University of Queensland, St. Lucia, QLD, 4072, Australia.
authors.forename: Kirill
authors.initials: K
authors.lastname: Alexandrov
authors.affiliation: Institute for Molecular Biosciences, University of Queensland, St. Lucia, QLD, 4072, Australia.
authors.forename: Jenny Lea
authors.initials: JL
authors.lastname: Stow
authors.affiliation: Institute for Molecular Biosciences, University of Queensland, St. Lucia, QLD, 4072, Australia.
authors.forename: Rohan David
authors.initials: RD
authors.lastname: Teasdale
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000502:physiology*; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006367:HeLa Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D009687:Nuclear Proteins; D011485:Protein Binding; D021381:Protein Transport; D012486:Salmonella typhimurium / Q000502:physiology*; D014617:Vacuoles / Q000382:microbiology; D020691:rab GTP-Binding Proteins / Q000378:metabolism*; D000090802:rab7 GTP-Binding Proteins
chemical_list: D001426:Bacterial Proteins; D009687:Nuclear Proteins; C567953:RAB34 protein, human; D000090802:rab7 GTP-Binding Proteins; C000716463:rab7 GTP-binding proteins, human; D020691:rab GTP-Binding Proteins
keywords: Rab34; Salmonella; SopD2; host-pathogen interaction