title: How to Successfully Screen Random Adeno-Associated Virus Display Peptide Libraries In Vivo.
abstract: Adeno-associated virus (AAV) has emerged as a very promising gene therapy vector. To enable tissue-directed gene expression, many artificially generated AAV variants have been established, often isolated from large pools of mutated capsids. Random peptide libraries displayed on AAV capsids have been used successfully to select vectors targeted to a given target cell or tissue in vitro and in vivo. However, the published methodology for screening of AAV libraries to isolate vectors with selective tissue tropism after intravenous administration in vivo has not been described in sufficient detail to address all critical steps. A step-by-step protocol is provided here.
authors.affiliation: 1 Department of Oncology and Hematology, University Cancer Center, University Medical Center Hamburg-Eppendorf , Hamburg, Germany .
authors.forename: Jakob
authors.initials: J
authors.lastname: Körbelin
authors.affiliation: 1 Department of Oncology and Hematology, University Cancer Center, University Medical Center Hamburg-Eppendorf , Hamburg, Germany .|2 Department of Hematology and Oncology, Augsburg Medical Center , Augsburg, Germany .
authors.forename: Martin
authors.initials: M
authors.lastname: Trepel
mesh_terms: D000818:Animals; D002460:Cell Line; D000229:Dependovirus / Q000235:genetics* / Q000378:metabolism; D018390:Gene Targeting / Q000379:methods*; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics; D051379:Mice; D019151:Peptide Library; D051381:Rats; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D019151:Peptide Library
keywords: AAV library; AAV selection; in vivo screening