title: Proteomic Profiling Analysis Reveals a Link between Experimental Autoimmune Uveitis and Complement Activation in Rats.
abstract: Uveitis is an autoimmune disease that usually damages the vision function, leading to poor visual quality in patients. As an autoimmune ocular inflammatory disease, the pathogenesis of uveitis is associated with abnormal expression of some proteins and aberrant regulation of multiple signalling pathways. Nevertheless, the detailed mechanism remains unclear. In this study, we induced an experimental autoimmune uveitis (EAU) model in rats. We determined the levels of C3a and membrane attack complex C5b-9 (soluble C5b-9, sC5b-9) in both plasma and aqueous humour, identified the differentially expressed proteins in plasma by liquid chromatography-tandem mass spectrometry and employed bioinformatics algorithms to analyse differentially expressed proteins in EAU rat plasma. The results demonstrate that there were 168 differentially expressed plasma proteins in EAU rats versus control subjects. The levels of sC5b-9 and C3a were elevated in the plasmas and aqueous humours of EAU rats. Gene ontology enrichment analysis showed that the differentially expressed proteins in EAU rat plasma were mainly involved in metabolic and immune processes. Kyoto encyclopedia of genes and genomes (KEGG) pathway annotation, database for annotation, visualization and integrated discovery (DAVID) and protein-protein interaction analyses revealed that the differentially expressed proteins in EAU rat plasmas were closely associated with complement and coagulation cascades, metabolic pathways, NF-kappa B, PI3K-Akt, Toll-like receptors and autophagy. Overall, the differentially expressed proteins in EAU rat plasmas are mainly involved in the complement and coagulation cascades. The pathogenesis of uveitis closely correlates with complement activation.
authors.affiliation: Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases in Universities of Shandong, Eye Institute of Shandong University of Traditional Chinese Medicine, Jinan, China.
authors.forename: D D
authors.initials: DD
authors.lastname: Guo
authors.affiliation: Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Jinan, China.
authors.forename: B
authors.initials: B
authors.lastname: Hu
authors.affiliation: The Second Clinical Medical College, Shandong University of Traditional Chinese Medicine, Jinan, China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Tang
authors.affiliation: The Second Clinical Medical College, Shandong University of Traditional Chinese Medicine, Jinan, China.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Sun
authors.affiliation: The Second Clinical Medical College, Shandong University of Traditional Chinese Medicine, Jinan, China.
authors.forename: B
authors.initials: B
authors.lastname: Liu
authors.affiliation: Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Jinan, China.
authors.forename: Q M
authors.initials: QM
authors.lastname: Tian
authors.affiliation: Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases in Universities of Shandong, Eye Institute of Shandong University of Traditional Chinese Medicine, Jinan, China.|Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Jinan, China.
authors.forename: H S
authors.identifier: http://orcid.org/0000-0002-1712-0055
authors.initials: HS
authors.lastname: Bi
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D002853:Chromatography, Liquid; D003167:Complement Activation; D015926:Complement C3a / Q000378:metabolism; D015938:Complement Membrane Attack Complex / Q000378:metabolism; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D060066:Protein Interaction Maps; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D011917:Rats, Inbred Lew; D053719:Tandem Mass Spectrometry; D014605:Uveitis / Q000097:blood / Q000276:immunology / Q000378:metabolism*
chemical_list: D015938:Complement Membrane Attack Complex; D020543:Proteome; D015926:Complement C3a