title: Association of platelet-activating factor receptor gene rs5938 (G/T) and rs313152 (T/C) polymorphisms with coronary heart disease and blood stasis syndrome in a Chinese Han population.
abstract: OBJECTIVE
  To explore the association of the platelet-activating factor receptor (PAFR) gene rs5938, rs313152 and rs76744145 polymorphisms with coronary heart disease (CHD) and blood stasis syndrome (BSS) of CHD in Chinese Han population.
  
  
  METHODS
  A total of 570 CHD patients (299 with BSS and 271 with non-BSS) and 317 controls were enrolled. The PAFR gene rs5938, rs313152 and rs76744145 polymorphisms were genotyped using the multiplex SNaPshot technology. The statistical analysis was conducted using a multiple variable logistic regression model.
  
  
  RESULTS
  Significant differences were detected in the genotypes frequency distributions of the rs5938 (P<0.01), but not the rs313152 (P>0.05), between the controls and CHD patients. Individuals with an rs5938 or rs313152 mutated allele had a low risk for CHD [adjusted odds ratio (aOR)=0.35, 95% confidence interval (CI): 0.23 to 0.56, P<0.01; aOR=0.65, 95% CI: 0.46 to 0.91, P<0.05, respectively]. After the CHD patients were stratified as BSS or non-BSS according to their Chinese medicine patterns, the rs5938 polymorphism mutated alleles had a significant association with a low risk for BSS of CHD (aOR=0.32, 95% CI: 0.18 to 0.57, P<0.01) and non-BSS of CHD (aOR=0.31, 95% CI: 0.17 to 0.55, P<0.01). The rs313152 polymorphism was associated with a low risk for BSS (aOR=0.51, 95% CI: 0.33 to 0.79, P<0.01), but not for non-BSS (aOR=1.22, 95% CI: 0.81 to 1.85, P<0.05). Furthermore, the interaction effect of the rs5938 and rs313152 polymorphisms for BSS of CHD was significantly based on an aOR value associated with the combination of the rs5938 GT genotype with the rs313152 TC genotype of 0.27 (95% CI: 0.1 to 0.7, P<0.01).
  
  
  CONCLUSIONS
  The PAFR gene rs5938 or rs313152 polymorphisms might be a potential biomarker for susceptibility to CHD, especially to BSS of CHD in Chinese Han population.
authors.affiliation: College of Health Information Technology and Management, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China. zhgh_1969@aliyun.com.
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Zheng
authors.affiliation: Department of Cardiologic Medicine, The Second People's Hospital of Fujian Province, Fuzhou, 350007, China.
authors.forename: Shang-Quan
authors.initials: SQ
authors.lastname: Xiong
authors.affiliation: The Second Clinic of Chinese Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China.
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Chen
authors.affiliation: The Second Clinic of Chinese Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Mei
authors.affiliation: Department of Cardiologic Medicine, The Second People's Hospital of Fujian Province, Fuzhou, 350007, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
mesh_terms: D044466:Asian People / Q000235:genetics*; D003327:Coronary Disease / Q000235:genetics*; D003710:Demography; D004843:Epistasis, Genetic; D005006:Ethnicity / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D010980:Platelet Membrane Glycoproteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics*; D013577:Syndrome
chemical_list: D010980:Platelet Membrane Glycoproteins; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor
keywords: Chinese medicine; blood stasis syndrome; coronary heart disease; gene polymorphism; platelet-activating factor receptor; susceptibility