title: Novel strategy to improve vanillin tolerance and ethanol fermentation performances of Saccharomycere cerevisiae strains.
abstract: The aim of this work was to develop a novel strategy for improving the vanillin tolerance and ethanol fermentation performances of Saccharomyces cerevisiae strains. Isogeneic diploid, triploid, and tetraploid S. cerevisiae strains were generated by genome duplication of haploid strain CEN.PK2-1C. Ploidy increments improved vanillin tolerance and diminished proliferation capability. Antimitotic drug methyl benzimidazol-2-ylcarbamate (MBC) was used to introduce chromosomal aberrations into the tetraploid S. cerevisiae strain. Interestingly, aneuploid mutants with DNA contents between triploid and tetraploid were more resistant to vanillin and showed faster ethanol fermentation rates than all euploid strains. The physiological characteristics of these mutants suggest that higher bioconversion capacities of vanillin and ergosterol contents might contribute to improved vanillin tolerance. This study demonstrates that genome duplication and MBC treatment is a powerful strategy to improve the vanillin tolerance of yeast strains.
authors.affiliation: College of Life Science, Zhejiang University, Hangzhou 310058, Zhejiang Province, China; Ocean College, Zhejiang University, Zhoushan, Zhejiang Province, China.
authors.forename: Dao-Qiong
authors.initials: DQ
authors.lastname: Zheng
authors.affiliation: College of Life Science, Zhejiang University, Hangzhou 310058, Zhejiang Province, China.
authors.forename: Xin-Na
authors.initials: XN
authors.lastname: Jin
authors.affiliation: College of Life Science, Zhejiang University, Hangzhou 310058, Zhejiang Province, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zhang
authors.affiliation: College of Life Science, Zhejiang University, Hangzhou 310058, Zhejiang Province, China.
authors.forename: Ya-Hong
authors.initials: YH
authors.lastname: Fang
authors.affiliation: College of Life Science, Zhejiang University, Hangzhou 310058, Zhejiang Province, China. Electronic address: mblab@163.com.
authors.forename: Xue-Chang
authors.initials: XC
authors.lastname: Wu
mesh_terms: D000782:Aneuploidy; D001547:Benzaldehydes / Q000633:toxicity*; D018533:Biomass; D004875:Ergosterol / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects*; D016681:Genome, Fungal; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D011003:Ploidies; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D001547:Benzaldehydes; D000431:Ethanol; C100058:vanillin; D004875:Ergosterol
keywords: Bioethanol; Ploidy; Saccharomyces cerevisiae; Tolerance; Vanillin