title: Complete genome sequence and analysis of blackcurrant leaf chlorosis associated virus, a new member of the genus Idaeovirus.
abstract: Blackcurrant leaf chlorosis associated virus (BCLCaV) was isolated from symptomatic blackcurrants (Ribes nigrum cv. Baldwin). The virus has a genome organization similar to that of raspberry bushy dwarf virus (RBDV), the type member of the genus Idaeovirus. The RNA-1of this virus encodes the replicase complex (ORF1, Mr197 kDa), while RNA-2 encodes a putative movement protein (ORF2a, Mr38.8 kDa) and the putative coat protein (ORF2b, Mr30 kDa). A concatenated form of BCLCaV RNA-2 was detected by next-generation sequencing and confirmed by RT-PCR. BCLCaV is a new member of the genus Idaeovirus.
authors.affiliation: Sidney Laboratory, Centre for Plant Health, Canadian Food Inspection Agency, 8801 East Saanich Road, North Saanich, BC, V8L 1H3, Canada. Delano.James@inspection.gc.ca.
authors.forename: Delano
authors.initials: D
authors.lastname: James
authors.affiliation: Sidney Laboratory, Centre for Plant Health, Canadian Food Inspection Agency, 8801 East Saanich Road, North Saanich, BC, V8L 1H3, Canada.
authors.forename: James
authors.initials: J
authors.lastname: Phelan
mesh_terms: D001483:Base Sequence; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D010942:Plant Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012328:RNA Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics; D031965:Ribes / Q000821:virology*
chemical_list: D012367:RNA, Viral