title: Fabrication and functional attributes of lipidic nanoconstructs of lycopene: An innovative endeavour for enhanced cytotoxicity in MCF-7 breast cancer cells.
abstract: The present study investigates the anticancer efficacy of lycopene loaded lipidic nanostructured particles. With a homogenization-evaporation technique, lycopene loaded solid lipid nanoparticles (LYC-SLNs) were fabricated with different ratios of biocompatible viz. compritol ATO 888 and gelucire, and evaluated for their micromeretics properties, in vitro release, in vitro cytotoxicity, cellular uptake and apoptosis induced in MCF-7 cells. Effects of anticancer potential of LYC-SLNs on the efficacy of methotrexate (MTX), a well-established anticancer agent, was evaluated thereafter. Cell culture experiments revealed a considerably higher cellular uptake of LYC-SLNs in MCF-7 cells, as compared to the free LYC. The concentration and time dependent cell survival of MCF-7 cells was significantly reduced by LYC-SLNs, as compared to their free LYC counterparts. Additionally, the combined cytotoxicity of the LYC and its nanostructured formulation with MTX was evaluated. The results of cytotoxicity and apoptotic study revealed synergism at all-time points up to 48h. In a nutshell, the combination regimen could be promising approach to improve the therapeutic benefits of anticancer agents.
authors.affiliation: University Institute of Pharmaceutical Sciences, UGC-Centre of Advanced Studies, Panjab University, Chandigarh 160 014, India; UGC-Centre of Excellence in Applications of Nanomaterials, Nanoparticles and Nanocomposites, Panjab University, Chandigarh 160 014, India; Dr. S. S. Bhatnagar University Institute of Chemical Engineering & Technology, Panjab University, Chandigarh 160 014, India.
authors.forename: Ashay
authors.initials: A
authors.lastname: Jain
authors.affiliation: University Institute of Pharmaceutical Sciences, UGC-Centre of Advanced Studies, Panjab University, Chandigarh 160 014, India.
authors.forename: Gajanand
authors.initials: G
authors.lastname: Sharma
authors.affiliation: Centre for Pharmaceutical Nanotechnology, Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research, SAS Nagar, Punjab 160062, India.
authors.forename: Varun
authors.initials: V
authors.lastname: Kushwah
authors.affiliation: University Institute of Pharmaceutical Sciences, UGC-Centre of Advanced Studies, Panjab University, Chandigarh 160 014, India.
authors.forename: Kanika
authors.initials: K
authors.lastname: Thakur
authors.affiliation: Dr. S. S. Bhatnagar University Institute of Chemical Engineering & Technology, Panjab University, Chandigarh 160 014, India. Electronic address: gargighoshal@yahoo.co.in.
authors.forename: Gargi
authors.initials: G
authors.lastname: Ghoshal
authors.affiliation: Dr. S. S. Bhatnagar University Institute of Chemical Engineering & Technology, Panjab University, Chandigarh 160 014, India.
authors.forename: Bhupinder
authors.initials: B
authors.lastname: Singh
authors.affiliation: Centre for Pharmaceutical Nanotechnology, Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research, SAS Nagar, Punjab 160062, India.
authors.forename: Sanyog
authors.initials: S
authors.lastname: Jain
authors.affiliation: Dr. S. S. Bhatnagar University Institute of Chemical Engineering & Technology, Panjab University, Chandigarh 160 014, India.
authors.forename: U S
authors.initials: US
authors.lastname: Shivhare
authors.affiliation: University Institute of Pharmaceutical Sciences, UGC-Centre of Advanced Studies, Panjab University, Chandigarh 160 014, India. Electronic address: katare@pu.ac.in.
authors.forename: O P
authors.initials: OP
authors.lastname: Katare
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002338:Carotenoids / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry; D005260:Female; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D000077276:Lycopene; D061986:MCF-7 Cells; D008727:Methotrexate / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D008055:Lipids; D002338:Carotenoids; D000077276:Lycopene; D008727:Methotrexate
keywords: Breast cancer; Cytotoxicity; Lycopene; Methotrexate; Solid lipid nanoparticles