title: Simultaneous quantification of reparixin and paclitaxel in plasma and urine using ultra performance liquid chromatography-tandem mass spectroscopy (UHPLC-MS/MS): Application to a preclinical pharmacokinetic study in rats.
abstract: A liquid chromatography-tandem mass spectroscopy (LC-MS/MS) assay was developed and validated to simultaneously quantify anticancer drugs reparixin and paclitaxel in this study. The compounds were extracted from plasma and urine samples by protein precipitation with acetone (supplemented with 0.1% formic acid). Chromatographic separation was achieved using a C18 column, and drug molecules were ionized using dual ion source electrospray and atmospheric pressure chemical ionization (DUIS: ESI-APCI). Reparixin and paclitaxel were quantified using negative and positive multiple reaction monitoring (MRM) mode, respectively. Stable isotope palcitaxel-D5 was used as the internal standard (IS). The assay was validated for specificity, recovery, carryover and sample stability under various storage conditions; it was also successfully applied to measure drug concentrations collected from a pharmacokinetic study in rats. The results confirmed that the assay was accurate and simple in quantifying both reparixin and paclitaxel in plasma and urine with minimal sample pretreatment.
authors.affiliation: College of Pharmacy, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, R3E 0T5, Canada.
authors.forename: Sarandeep
authors.initials: S
authors.lastname: Malhi
authors.affiliation: College of Pharmacy, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, R3E 0T5, Canada.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Stesco
authors.affiliation: College of Pharmacy, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, R3E 0T5, Canada.
authors.forename: Samaa
authors.initials: S
authors.lastname: Alrushaid
authors.affiliation: College of Pharmacy, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, R3E 0T5, Canada.
authors.forename: Ted M
authors.initials: TM
authors.lastname: Lakowski
authors.affiliation: College of Pharmacy, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, R3E 0T5, Canada.
authors.forename: Neal M
authors.initials: NM
authors.lastname: Davies
authors.affiliation: College of Pharmacy, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, R3E 0T5, Canada. Electronic address: Xiaochen.Gu@umanitoba.ca.
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Gu
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016014:Linear Models; D008297:Male; D017239:Paclitaxel / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics / Q000652:urine; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013449:Sulfonamides / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics / Q000652:urine; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D013449:Sulfonamides; D017239:Paclitaxel; C490707:reparixin
keywords: Paclitaxel; Pharmacokinetics; Reparixin; UHPLC–MS/MS