title: Predicted RNA Binding Proteins Pes4 and Mip6 Regulate mRNA Levels, Translation, and Localization during Sporulation in Budding Yeast.
abstract: In response to starvation, diploid cells ofSaccharomyces cerevisiaeundergo meiosis and form haploid spores, a process collectively referred to as sporulation. The differentiation into spores requires extensive changes in gene expression. The transcriptional activator Ndt80 is a central regulator of this process, which controls many genes essential for sporulation. Ndt80 induces ∼300 genes coordinately during meiotic prophase, but different mRNAs within theNDT80regulon are translated at different times during sporulation. The protein kinase Ime2 and RNA binding protein Rim4 are general regulators of meiotic translational delay, but how differential timing of individual transcripts is achieved was not known. This report describes the characterization of two relatedNDT80-induced genes,PES4andMIP6, encoding predicted RNA binding proteins. These genes are necessary to regulate the steady-state expression, translational timing, and localization of a set of mRNAs that are transcribed byNDT80but not translated until the end of meiosis II. Mutations in the predicted RNA binding domains withinPES4alter the stability of target mRNAs.PES4andMIP6affect only a small portion of theNDT80regulon, indicating that they act as modulators of the general Ime2/Rim4 pathway for specific transcripts.
authors.affiliation: Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York, USA.
authors.forename: Liang
authors.initials: L
authors.lastname: Jin
authors.affiliation: Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York, USA.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York, USA.
authors.forename: Rolf
authors.initials: R
authors.lastname: Sternglanz
authors.affiliation: Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York, USA aaron.neiman@stonybrook.edu.
authors.forename: Aaron M
authors.initials: AM
authors.lastname: Neiman
mesh_terms: D018797:Cell Cycle Proteins / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008540:Meiosis / Q000502:physiology; D014176:Protein Biosynthesis / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D013172:Spores, Fungal / Q000166:cytology* / Q000378:metabolism; D013217:Starvation; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; C000618378:Mip6 protein, S cerevisiae; C097071:NDT80 protein, S cerevisiae; C000618376:Pes4 protein, S cerevisiae; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C000604348:Rim4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C064979:IME2 protein, S cerevisiae; D017346:Protein Serine-Threonine Kinases
keywords: RRM domain; mRNA localization; mRNA stability; sporulation; translational control