title: An anthocyanin rich strawberry extract induces apoptosis and ROS while decreases glycolysis and fibrosis in human uterine leiomyoma cells.
abstract: Uterine leiomyomas are highly prevalent benign tumors in reproductive aged women. Unfortunately, medical treatments are still limited and no preventive therapies have been developed. In the present study, we investigated the therapeutic effects of strawberry extract on uterine leiomyoma cells. Leiomyoma and myometrial cells were treated with strawberry (cultivar Alba) extract (250 μg/ml) for 48 h to measure apoptosis, reactive oxygen species (ROS), oxidative phosphorylation (OCR, oxygen consumption rate) and glycolysis (ECAR, extracellular acidification rate) as well as fibrosis associated gene and/or protein expression. In leiomyoma cells, strawberry increased the percentage of apoptotic and dead cells. Strawberry significantly increased ROS concentration in leiomyoma cells, while decreased it in myometrial cells. After strawberry treatment, leiomyoma cells showed a significant decreased rate of ECAR, while OCR was unchanged in both myometrial and leiomyoma cells. Strawberry significantly decreased collagen1A1, fibronectin and versican mRNA expression in leiomyoma cells. The reduced protein expression of fibronectin was observed by strawberry extract in leiomyoma cells as well. Furthermore, strawberry was able to reduce activin A induced fibronectin, collagen1A1, and versican as well as activin A and PAI-1 mRNA expression in leiomyoma cells. This study suggests that strawberry can be developed as therapeutic and/or preventive agent for uterine leiomyomas.
authors.affiliation: Department of Experimental and Clinical Medicine, Università Politecnica delle Marche, Ancona, Italy.|Biotechnology and Microbiology Laboratory, Department of Botany, University of Rajshahi, Rajshahi, Bangladesh.
authors.forename: Md Soriful
authors.initials: MS
authors.lastname: Islam
authors.affiliation: Department of Clinical Science, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Giampieri
authors.affiliation: Department of Experimental and Clinical Medicine, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Milijana
authors.initials: M
authors.lastname: Janjusevic
authors.affiliation: Department of Clinical Science, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Gasparrini
authors.affiliation: Department of Clinical Science, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Tamara Y
authors.initials: TY
authors.lastname: Forbes-Hernandez
authors.affiliation: Department of Clinical Science, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Mazzoni
authors.affiliation: Department of Experimental and Clinical Medicine, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Greco
authors.affiliation: Department of Clinical Science, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Stefano Raffaele
authors.initials: SR
authors.lastname: Giannubilo
authors.affiliation: Department of Clinical Science, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Ciavattini
authors.affiliation: Department of Agricultural, Food and Environmental Sciences, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Bruno
authors.initials: B
authors.lastname: Mezzetti
authors.affiliation: Department of Agricultural, Food and Environmental Sciences, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Franco
authors.initials: F
authors.lastname: Capocasa
authors.affiliation: Department of Experimental and Clinical Medicine, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Castellucci
authors.affiliation: Department of Clinical Science, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Battino
authors.affiliation: Department of Experimental and Clinical Medicine, Università Politecnica delle Marche, Ancona, Italy.|Department of Information Engineering, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Pasquapina
authors.initials: P
authors.lastname: Ciarmela
mesh_terms: D028341:Activins / Q000235:genetics / Q000378:metabolism; D000872:Anthocyanins / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002470:Cell Survival / Q000187:drug effects; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D000090602:Collagen Type I, alpha 1 Chain; D005260:Female; D005355:Fibrosis; D031985:Fragaria / Q000737:chemistry*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006019:Glycolysis / Q000187:drug effects*; D006801:Humans; D007889:Leiomyoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D053675:Versicans / Q000235:genetics / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D010936:Plant Extracts; D017395:Plasminogen Activator Inhibitor 1; D017382:Reactive Oxygen Species; C422405:activin A; D028341:Activins; D053675:Versicans
keywords: ROS; apoptosis; fibrosis; strawberry; uterine leiomyoma