title: Stromal vascular fraction shows robust wound healing through high chemotactic and epithelialization property.
abstract: Although human stromal vascular fraction (SVF) has been regarded as an attractive stem cell source, its therapeutic mechanism in wound healing has not been fully elucidated.
  
  
  
  In this study, we investigated the molecular characteristics and therapeutic property of SVF for wound healing.
  
  
  
  Microarray data showed that SVF cells are enriched with a higher level of wound healing or epithelium development-related genes and micro RNA.
  
  
  
  Quantitative polymerase chain reaction (PCR) and reverse transcriptase PCR results revealed that the epithelialization growth factor, epidermal growth factor (EGF), chemokines, stromal cell-derived factor (SDF-1 or CXCL12), neutrophil-activating protein-2 (NAP-2 or CXCL7), chemokine receptors (CXCR1, CCR2 and CCR3) and wound healing genes were up-regulated in SVF compared with those in adipose-derived mesenchymal stem cells (ASCs). An in vitro scratch wound closure experiment demonstrated that co-culture with SVF substantially accelerated the wound closure of fibroblasts. Wounds in nude mice were created by skin excisions followed by injections of SVF with Pluronic hydrogel. SVF implantation highly accelerated wound closure and increased cellularity and re-epithelialization. In addition, the transplanted SVF exhibited high engraftment rates in the wound area, suggesting direct benefits for cutaneous closure.
  
  
  
  Taken together, these data suggest that SVF possesses high therapeutic capability for wound healing via the secretion of epithelialization and chemotactic growth factors and enhanced engraftment properties.
authors.affiliation: Department of Orthopedic Surgery, International St. Mary's Hospital, Catholic Kwandong University College of Medicine, Incheon, Republic of Korea.
authors.forename: Dong-Sic
authors.initials: DS
authors.lastname: Chae
authors.affiliation: Department of Family Medicine, College of Medicine, Dong-A University, Busan, Republic of Korea.
authors.forename: Seongho
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Medicine, College of Medicine, Catholic Kwandong University, Gangneung, Republic of Korea.
authors.forename: Mina
authors.initials: M
authors.lastname: Son
authors.affiliation: Department of Medicine, College of Medicine, Catholic Kwandong University, Gangneung, Republic of Korea. Electronic address: swkim@cku.ac.kr.
authors.forename: Sung-Whan
authors.initials: SW
authors.lastname: Kim
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000166:cytology*; D002478:Cells, Cultured; D002633:Chemotaxis; D018920:Coculture Techniques; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D005786:Gene Expression Regulation; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D008819:Mice, Nude; D062326:Re-Epithelialization / Q000235:genetics / Q000502:physiology*; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology* / Q000637:transplantation*; D015854:Up-Regulation
chemical_list: D036341:Intercellular Signaling Peptides and Proteins
keywords: Pluronic hydrogel; chemokine; epidermal growth factor; stromal vascular fraction; wound healing