title: Kinetic analysis and degradation pathway for m-dichlorobenzene removal by Brevibacillus agri DH-1 and its performance in a biotrickling filter.
abstract: A strain, Brevibacillus agri DH-1, isolated from dry lands was used to remove m-dichlorobenzene. After 48h culturing, the concentrations of m-dichlorobenzene decreased from 26-130 to 7.87-28.87mg/L and dry cell weight for bacterial growth reached 52.43-75.05mg/L. The growth and degradation kinetics were analyzed by the fitting of Haldane-Andrews model and pseudo first-order model. A degradation pathway was proposed according to major intermediates (phenol), chloride ion variation, ring-opening enzyme activity, and high mineralization (0.47gCl-/gm-dichlorobenzene, 0.65 gco2/gm-dichlorobenzene, 0.15 gDCW/gm-dichlorobenzene). In addition, the performance in a biotrickling filter (BTF) was evaluated through removal efficiency and pressure drop values with increasing inlet loading rate from 4.10 to 122.57g/m3/h at three empty bed residence time points (30s, 60s, and 90s). The results demonstrated that strain DH-1 possessed high removal efficiency and stable operation in a BTF.
authors.affiliation: School of Environment Science and Spatial Informatics, China University of Mining and Technology, Xuzhou, Jiangsu 221116, China; School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu 224051, China.
authors.forename: Bai-Ren
authors.initials: BR
authors.lastname: Yang
authors.affiliation: School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu 224051, China.
authors.forename: Zhu-Qiu
authors.initials: ZQ
authors.lastname: Sun
authors.affiliation: School of Environment Science and Spatial Informatics, China University of Mining and Technology, Xuzhou, Jiangsu 221116, China. Electronic address: wlpkd123@163.com.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Wang
authors.affiliation: School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu 224051, China.
authors.forename: Zhao-Xia
authors.initials: ZX
authors.lastname: Li
authors.affiliation: School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu 224051, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Ding
mesh_terms: D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology; D058675:Brevibacillus / Q000254:growth & development / Q000378:metabolism*; D002245:Carbon Dioxide / Q000032:analysis; D002712:Chlorides / Q000032:analysis; D002722:Chlorobenzenes / Q000302:isolation & purification*; D005374:Filtration / Q000295:instrumentation*; D007700:Kinetics; D011312:Pressure
chemical_list: D002712:Chlorides; D002722:Chlorobenzenes; D002245:Carbon Dioxide; C042146:1,3-dichlorobenzene
keywords: Biotrickling filter; Degradation pathway; Kinetic analysis; m-Dichlorobenzene