title: Near-Infrared Probe Based on Rhodamine Derivative for Highly Sensitive and Selective Lysosomal pH Tracking.
abstract: The development of near-infrared fluorescent probes with low pKa, high selectivity, high photostability, and high sensitivity for lysosomal pH detection is of great importance. In the present work, we developed a novel near-infrared lysosomal pH probe (Lyso-hNR) based on a rhodamine derivative. Lyso-hNR showed fast, highly sensitive, and highly selective fluorescence response to acidic pH caused by the H+-induced structure changes from the nonfluorescent spirolactam form to the highly emissive open-ring form. Lyso-hNR displays a significant fluorescence enhancement at 650 nm (over 280-fold) from pH 7.0 to 4.0 with a pKavalue of 5.04. Live cell imaging data revealed that Lyso-hNR can selectively monitor lysosomal pH changes with excellent photostability and low cytotoxicity. In addition, Lyso-hNR can be successfully used in tracking lysosomal pH changes induced by chloroquine and those during apoptosis. All these features render Lyso-hNR a promising candidate to investigate lysosome-associated physiological and pathological processes.
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Beijing, 100190, China.|School of Future Technology, University of Chinese Academy of Sciences , Beijing, 100049, China.
authors.forename: Guangle
authors.identifier: 0000-0002-5403-6880
authors.initials: G
authors.lastname: Niu
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Beijing, 100190, China.|Institute of Functional Nano and Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University , Suzhou, Jiangsu 215123, China.
authors.forename: Panpan
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Beijing, 100190, China.|School of Future Technology, University of Chinese Academy of Sciences , Beijing, 100049, China.
authors.forename: Weimin
authors.identifier: 0000-0001-7507-6032
authors.initials: W
authors.lastname: Liu
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Beijing, 100190, China.
authors.forename: Mengqi
authors.initials: M
authors.lastname: Wang
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Beijing, 100190, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Beijing, 100190, China.
authors.forename: Jiasheng
authors.initials: J
authors.lastname: Wu
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Beijing, 100190, China.|School of Future Technology, University of Chinese Academy of Sciences , Beijing, 100049, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Beijing, 100190, China.|School of Future Technology, University of Chinese Academy of Sciences , Beijing, 100049, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Wang
mesh_terms: D000072283:A549 Cells; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D002470:Cell Survival / Q000187:drug effects; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007769:Lactams / Q000737:chemistry; D057230:Limit of Detection; D008247:Lysosomes / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D066244:Proton Magnetic Resonance Spectroscopy; D012235:Rhodamines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D007769:Lactams; D012235:Rhodamines