title: Silica - Boronate affinity material for quick enrichment of intracellular nucleosides.
abstract: Boronic acid modified materials have been widely used to adsorb nucleosides, but their adsorption capacities require further improvement. Most cis-diol containing biomolecules are in very low abundance along with interfering components in real samples, and need to be enriched specially. In this study, we synthesize a kind of silica absorbent modified with boronic acid derivative, using amorphous silica as raw material and obtaining high adsorption capacity for adenosine. In addition, the adsorption equilibrium can be completed within 10s and 1min for the desorption. Finally, the material was successfully applied to enrich nucleosides from cells and the spiked recoveries were found between 82.21% and 118.9%. The results showed that the prepared adsorbent has potential to effectively enrich cis-diol substances from cell samples.
authors.affiliation: Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Shuxia
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Guan
authors.affiliation: Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China. Electronic address: zhanghx@lzu.edu.cn.
authors.forename: Haixia
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D001897:Boronic Acids / Q000737:chemistry*; D002460:Cell Line; D042541:Intracellular Space / Q000737:chemistry*; D009705:Nucleosides / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D001897:Boronic Acids; D009705:Nucleosides; D012822:Silicon Dioxide
keywords: Boronic acid; Cells; HPLC; Nucleosides; Silica