title: Effects of Static Cold Storage and Hypothermic Machine Perfusion on Oxidative Stress Factors, Adhesion Molecules, and Zinc Finger Transcription Factor Proteins Before and After Liver Transplantation.
abstract: BACKGROUND This study aimed to investigate the effects of static cold storage (SCS) and hypothermic machine perfusion (HMP) on the oxidative stress factors (OSF), adhesion molecules (AM), and zinc finger transcription factor (Snail) before and after liver transplantation. MATERIAL AND METHODS Experimental dogs were randomly divided into donor (group A), SCS (group B), and HMP (group C) (n=30) groups. Livers retrieved from group A were transplanted into group B after SCS, and the livers sampled from group B were transplanted into group C after HMP. The dogs in group A were euthanized and discarded, and the livers sampled from group C were used for other experiments. Twenty dogs with successful liver transplants were randomly selected from groups B and C for analysis. RESULTS During the liver sampling process, the levels of OSF, AM, and Snail between the 2 groups showed no significant differences (P>0.05); before the transplantation, the levels of chemokine CXCL14 and Snail between the 2 groups showed no significant differences (P>0.05), and compared with group B, HIF-1α and P-selectin in group C were lower (P<0.01); 60 min after the transplantation, HIF-1α, chemokine CXCL14, P-selectin, and Snail in group C were lower than that in group B (P<0.01). CONCLUSIONS HMP can significantly reduce the levels of OSF and inflammatory factors, which is conducive for liver transplantation.
authors.affiliation: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia, China (mainland).
authors.forename: De-Fang
authors.initials: DF
authors.lastname: Zhao
authors.affiliation: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia, China (mainland).
authors.forename: Qin
authors.initials: Q
authors.lastname: Dong
authors.affiliation: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia, China (mainland).
authors.forename: Tong
authors.initials: T
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D019743:Chemokines, CXC / Q000378:metabolism; D015925:Cryopreservation / Q000379:methods*; D004285:Dogs; D005240:Feasibility Studies; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D016031:Liver Transplantation; D008297:Male; D009926:Organ Preservation / Q000009:adverse effects / Q000379:methods*; D018384:Oxidative Stress; D019007:P-Selectin / Q000378:metabolism; D010477:Perfusion / Q000379:methods*; D011897:Random Allocation; D000071250:Snail Family Transcription Factors / Q000378:metabolism; D014183:Transplantation, Heterologous
chemical_list: D015415:Biomarkers; D015815:Cell Adhesion Molecules; D019743:Chemokines, CXC; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D019007:P-Selectin; D000071250:Snail Family Transcription Factors