title: Plasmonic Hepatitis B Biosensor for the Analysis of Clinical Saliva.
abstract: A biosensor for the detection of hepatitis B antibodies in clinical saliva was developed. Compared to conventional analysis of blood serum, it offers the advantage of noninvasive collection of samples. Detection of biomarkers in saliva imposes two major challenges associated with the low analyte concentration and increased surface fouling. The detection of minute amounts of hepatitis B antibodies was performed by plasmonically amplified fluorescence sandwich immunoassay. To have access to specific detection, we prevented the nonspecific adsorption of biomolecules present in saliva by brushes of poly[(N-(2-hydroxypropyl) methacrylamide)-co-(carboxybetaine methacrylamide)] grafted from the gold sensor surface and post modified with hepatitis B surface antigen. Obtained results were validated against the response measured with ELISA at a certified laboratory using serum from the same patients.
authors.affiliation: Institute of Macromolecular Chemistry AS CR v.v.i. , Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Riedel
authors.affiliation: Biosensor Technologies, AIT-Austrian Institute of Technology GmbH , Muthgasse 11, 1190 Vienna, Austria.
authors.forename: Simone
authors.initials: S
authors.lastname: Hageneder
authors.affiliation: Institute of Macromolecular Chemistry AS CR v.v.i. , Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic.
authors.forename: František
authors.initials: F
authors.lastname: Surman
authors.affiliation: Institute of Macromolecular Chemistry AS CR v.v.i. , Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic.
authors.forename: Ognen
authors.initials: O
authors.lastname: Pop-Georgievski
authors.affiliation: Molecular Diagnostics, Health and Environment Department, AIT-Austrian Institute of Technology GmbH , Muthgasse 11, 1190 Vienna, Austria.
authors.forename: Christa
authors.initials: C
authors.lastname: Noehammer
authors.affiliation: Molecular Diagnostics, Health and Environment Department, AIT-Austrian Institute of Technology GmbH , Muthgasse 11, 1190 Vienna, Austria.
authors.forename: Manuela
authors.initials: M
authors.lastname: Hofner
authors.affiliation: Institute of Macromolecular Chemistry AS CR v.v.i. , Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic.
authors.forename: Eduard
authors.initials: E
authors.lastname: Brynda
authors.affiliation: Institute of Macromolecular Chemistry AS CR v.v.i. , Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic.|DWI - Leibniz Institute for Interactive Materials and Institute of Technical and Macromolecular Chemistry, RWTH Aachen University , Forckenbeckstraße 50, 52074 Aachen, Germany.
authors.forename: Cesar
authors.identifier: 0000-0003-0745-0840
authors.initials: C
authors.lastname: Rodriguez-Emmenegger
authors.affiliation: Biosensor Technologies, AIT-Austrian Institute of Technology GmbH , Muthgasse 11, 1190 Vienna, Austria.
authors.forename: Jakub
authors.identifier: 0000-0002-0431-2170
authors.initials: J
authors.lastname: Dostálek
mesh_terms: D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006510:Hepatitis B Antibodies / Q000032:analysis* / Q000097:blood / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000276:immunology; D007118:Immunoassay; D011108:Polymers / Q000737:chemistry; D012463:Saliva / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D020349:Surface Plasmon Resonance
chemical_list: D015415:Biomarkers; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D055767:Immobilized Proteins; D011108:Polymers; D006046:Gold