title: Biotransformation of Rutin Using Crude Enzyme from Rhodopseudomonas palustris.
abstract: Rhodopseudomonas palustris was selected for the ability to grow in diglycosylated flavonoids-based media, exhibited deglycosylation activity. The present study aimed to investigate the ability of a crude enzyme from Rhodopseudomonas palustris to transform rutin. Our results showed the crude enzyme was found to transform rutin to quercetin via isoquercitrin. The maximum enzyme activities were observed at pH 7.0, 25 °C, and rutin concentration of 1.0 mg mL- 1. Under optimal conditions, 13.11 μM rutin was biotransformed into 6.86 μM isoquercitrin and 11.64 μM quercetin after 11 and 21 h, respectively. The study demonstrates an eco-friendly and potential economically viable 'green' conversion route to convert rutin to isoquercitrin and quercetin, which is of great interest, considering the therapeutic applications of isoquercitrin and quercetin. The specific biotransformation of rutin to isoquercitrin and quercetin, using the crude enzyme from Rhodopseudomonas palustris, may potentially serve as a new method for industrial production of isoquercitrin and quercetin.
authors.affiliation: School of Pharmacy, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
authors.forename: Chun-Jie
authors.initials: CJ
authors.lastname: Guan
authors.affiliation: School of Pharmacy, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
authors.forename: Yu-Jia
authors.initials: YJ
authors.lastname: Ji
authors.affiliation: School of Pharmacy, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
authors.forename: Jia-Lin
authors.initials: JL
authors.lastname: Hu
authors.affiliation: School of Pharmacy, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
authors.forename: Chao-Nan
authors.initials: CN
authors.lastname: Hu
authors.affiliation: School of Pharmacy, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Yang
authors.affiliation: School of Pharmacy, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China. yangguane@aliyun.com.
authors.forename: Guan-E
authors.initials: GE
authors.lastname: Yang
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D001711:Biotransformation; D011794:Quercetin / Q000031:analogs & derivatives / Q000378:metabolism; D012241:Rhodopseudomonas / Q000201:enzymology* / Q000378:metabolism*; D012431:Rutin / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; C016527:isoquercitrin; D012431:Rutin; D011794:Quercetin