title: Expression of mammalian mitochondrial F1-ATPase inEscherichia colidepends on two chaperone factors, AF1 and AF2.
abstract: F1-ATPase (F1) is a multisubunit water-soluble domain of FoF1-ATP synthase and is a rotary enzyme by itself. Earlier genetic studies using yeast suggested that two factors, Atp11p and Atp12p, contribute to F1assembly. Here, we show that their mammalian counterparts, AF1 and AF2, are essential and sufficient for efficient production of recombinant bovine mitochondrial F1inEscherichia colicells. Intactness of the function and conformation of theE. coli-expressed bovine F1was verified by rotation analysis and crystallization. This expression system opens a way for the previously unattempted mutation study of mammalian mitochondrial F1.
authors.affiliation: Faculty of Science and Engineering Waseda University Tokyo Japan; Department of Molecular Bioscience Kyoto-Sangyo University Kyoto Japan; Chemical Resources Laboratory Tokyo Institute of Technology Yokohama Japan; Present address: Department of Applied Chemistry School of Engineering The University of Tokyo Hongo, Bunkyo-ku Tokyo 113-8656 Japan.
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: Faculty of Science and Engineering Waseda University Tokyo Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Iida
authors.affiliation: Department of Molecular Bioscience Kyoto-Sangyo University Kyoto Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Suzuki
authors.affiliation: Department of Molecular Bioscience Kyoto-Sangyo University Kyoto Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Watanabe
authors.affiliation: Department of Molecular Bioscience Kyoto-Sangyo University Kyoto Japan.
authors.forename: Toshiya
authors.initials: T
authors.lastname: Endo
authors.affiliation: Chemical Resources Laboratory Tokyo Institute of Technology Yokohama Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Hisabori
authors.affiliation: Department of Molecular Bioscience Kyoto-Sangyo University Kyoto Japan.
authors.forename: Masasuke
authors.initials: M
authors.lastname: Yoshida
keywords: F1‐ATPase; FoF1‐ATP synthase; molecular chaperone