title: Systematic Study of Functionalizable, Non-Biofouling Agarose Films with Protein and Cellular Patterns on Glass Slides.
abstract: Herein we demonstrate a systematic investigation of chemically functionalizable, non-biofouling agarose films over large-area glass surfaces. Agarose films, prepared with various concentrations of aqueous agarose, were activated by using periodate oxidation to generate aldehyde groups at the termini of the agarose chains. The non-biofouling efficacy and binding capabilities of the activated films were evaluated by using protein and cellular patterning, performed by using a microarrayer, microcontact printing, and micromolding in capillaries. Characterization by using a fluorescence slide scanner and a scanning-probe microscope revealed that the pore sizes of the agarose films played an important role in achieving desirable film performance; the 0.2 wt % agarose film exhibited the optimum efficacy in this work.
authors.affiliation: Department of Chemistry and Green-Nano Materials Research Center, Kyungpook National University, Daegu, 41566, South Korea.
authors.forename: Gyeongyeop
authors.initials: G
authors.lastname: Han
authors.affiliation: Department of Chemistry and Center for Cell-Encapsulation, KAIST, Daejeon, 34141, South Korea.
authors.forename: Daehwa
authors.initials: D
authors.lastname: Hong
authors.affiliation: Department of Chemistry and Center for Cell-Encapsulation, KAIST, Daejeon, 34141, South Korea.
authors.forename: Bong Soo
authors.initials: BS
authors.lastname: Lee
authors.affiliation: Department of Chemistry and Green-Nano Materials Research Center, Kyungpook National University, Daegu, 41566, South Korea.
authors.forename: EunRae
authors.initials: E
authors.lastname: Ha
authors.affiliation: Department of Chemistry and Center for Cell-Encapsulation, KAIST, Daejeon, 34141, South Korea.
authors.forename: Ji Hun
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Chemistry and Center for Cell-Encapsulation, KAIST, Daejeon, 34141, South Korea.
authors.forename: Insung S
authors.initials: IS
authors.lastname: Choi
authors.affiliation: Department of Chemistry, Chungbuk National University, Cheongju, 28644, South Korea.
authors.forename: Sung Min
authors.initials: SM
authors.lastname: Kang
authors.affiliation: Department of Chemistry and Green-Nano Materials Research Center, Kyungpook National University, Daegu, 41566, South Korea.
authors.forename: Jungkyu K
authors.initials: JK
authors.lastname: Lee
mesh_terms: D057219:Biofouling; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000592:standards; D005453:Fluorescence; D005898:Glass / Q000737:chemistry*; D010316:Particle Size; D016062:Porosity; D011506:Proteins / Q000737:chemistry*; D012685:Sepharose / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D011506:Proteins; D012685:Sepharose
keywords: bioorganic chemistry; coating techniques; protein and cellular pattern; surface chemistry; thin films