title: A Review of Techniques to Measure Protein Sorption to Soft Contact Lenses.
abstract: OBJECTIVE
  To compare and critically evaluate a variety of techniques to measure the quantity and biological activity of protein sorption to contact lenses over short time periods.
  
  
  METHODS
  A literature review was undertaken investigating the major techniques to measure protein sorption to soft contact lens materials, with specific reference to measuring protein directly on lenses using in situ, ex situ, protein structural, and biological activity techniques.
  
  
  RESULTS
  The use of in situ techniques to measure protein quantity provides excellent sensitivity, but many are not directly applicable to contact lenses. Many ex situ techniques struggle to measure all sorbed proteins, and these measurements can have significant signal interference from the lens materials themselves. Techniques measuring the secondary and tertiary structures of sorbed proteins have exhibited only limited success.
  
  
  CONCLUSIONS
  There are a wide variety of techniques to measure both the amount of protein and the biological activity of protein sorbed to soft contact lens materials. To measure the mass of protein sorbed to soft contact lenses (not just thin films) over short time periods, the method of choice should be I radiolabeling. This technique is sensitive enough to measure small amounts of deposited protein, provided steps are taken to limit and measure any interaction of the iodine tracer with the materials. To measure the protein activity over short time periods, the method of choice should be to measure the biological function of sorbed proteins. This may require new methods or adaptations of existing ones.
authors.affiliation: Centre for Contact Lens Research (B.H., J.A.F., L.J.), School of Optometry and Vision Science University of Waterloo, Waterloo, ON, Canada; Department of Physics and Astronomy (J.A.F.), University of Waterloo, ON, Canada; Sengi Data (B.H.), Cambridge, ON, Canada; and Perimeter Institute for Theoretical Physics (J.A.F.), Waterloo, ON, Canada.
authors.forename: Brad
authors.initials: B
authors.lastname: Hall
authors.forename: James A
authors.initials: JA
authors.lastname: Forrest
authors.forename: Lyndon
authors.initials: L
authors.lastname: Jones
mesh_terms: D000327:Adsorption; D003263:Contact Lenses, Hydrophilic; D003941:Diagnostic Techniques, Ophthalmological; D005136:Eye Proteins / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding
chemical_list: D005136:Eye Proteins