title: Detection of glycoprotein through fluorescent boronic acid-based molecularly imprinted polymer.
abstract: A novel strategy based on the fluorescent molecularly imprinted polymers (FMIP) for specific recognition and sensitive sensing glycoprotein from biological samples was developed. The FMIP prepared by introducing a fluorescent boronic acid quinoline-based on the PGMA/EDMAbead surface and assembled a glycoprotein molecularly imprinted polymer using surface imprinting technology. The resultant material showed specific recognition to model glycoprotein. At the same time, the change of fluorescence caused by the amount of model glycoprotein could achieve quantitative determination. The method provided a good linear relationship of the concentrations of HRP in the range of 0.05-1 μM, and the detection limit was 0.02 μM. It is 10 times lower than the previous fluorescence nanosensor for glycoprotein. The established method combined the desired selectivity of molecularly imprinted polymers and high sensitivity of fluorescence spectroscopy. The influence of background impurities could be effectively eliminated. The outstanding features guarantee that it can be successfully applied to detect glycoprotein from biological samples under physiological conditions.
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, PR China.
authors.forename: Jing-Ru
authors.initials: JR
authors.lastname: Wei
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, PR China.
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Ni
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, PR China.
authors.forename: Zhi-Qi
authors.initials: ZQ
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, PR China. Electronic address: zhangjing8902@snnu.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
mesh_terms: D001897:Boronic Acids / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006023:Glycoproteins / Q000032:analysis* / Q000097:blood; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D054802:Molecular Imprinting; D011108:Polymers / Q000138:chemical synthesis*; D011804:Quinolines / Q000737:chemistry
chemical_list: D001897:Boronic Acids; D005456:Fluorescent Dyes; D006023:Glycoproteins; D011108:Polymers; D011804:Quinolines; C037219:quinoline
keywords: Fluorescent boronic acid; Glycoprotein; Molecularly imprinted polymer