title: Transcriptomic meta-analysis reveals up-regulation of gene expression functional in osteoclast differentiation in human septic shock.
abstract: Septic shock is a major medical problem with high morbidity and mortality and incompletely understood biology. Integration of multiple data sets into a single analysis framework empowers discovery of new knowledge about the condition that may have been missed by individual analysis of each of these datasets. Electronic search was performed on medical literature and gene expression databases for selection of transcriptomic studies done in circulating leukocytes from human subjects suffering from septic shock. Gene-level meta-analysis was conducted on the six selected studies to identify the genes consistently differentially expressed in septic shock. This was followed by pathway-level analysis using three different algorithms (ORA, GSEA, SPIA). The identified up-regulated pathway, Osteoclast differentiation pathway (hsa04380) was validated in two independent cohorts. Of the pathway, 25 key genes were selected that serve as an expression signature of Septic Shock.
authors.affiliation: National Institute of Biomedical Genomics, Kalyani, West Bengal, India.
authors.forename: Samanwoy
authors.initials: S
authors.lastname: Mukhopadhyay
authors.affiliation: Department of Medicine, Sriram Chandra Bhanja Medical College and Hospital, Cuttack, Odisha, India.
authors.forename: Pravat K
authors.initials: PK
authors.lastname: Thatoi
authors.affiliation: National Institute of Biomedical Genomics, Kalyani, West Bengal, India.
authors.forename: Abhay D
authors.initials: AD
authors.lastname: Pandey
authors.affiliation: Department of Medicine, Sriram Chandra Bhanja Medical College and Hospital, Cuttack, Odisha, India.
authors.forename: Bidyut K
authors.initials: BK
authors.lastname: Das
authors.affiliation: Institute of Life Sciences, NALCO Square, Bhubaneswar, Odisha, India.
authors.forename: Balachandran
authors.initials: B
authors.lastname: Ravindran
authors.affiliation: National Institute of Biomedical Genomics, Kalyani, West Bengal, India.
authors.forename: Samsiddhi
authors.initials: S
authors.lastname: Bhattacharjee
authors.affiliation: National Institute of Biomedical Genomics, Kalyani, West Bengal, India.
authors.forename: Saroj K
authors.initials: SK
authors.lastname: Mohapatra
mesh_terms: D000465:Algorithms; D002454:Cell Differentiation / Q000235:genetics*; D016208:Databases, Factual; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism; D008954:Models, Biological; D010010:Osteoclasts / Q000166:cytology* / Q000378:metabolism; D025341:Principal Component Analysis; D012772:Shock, Septic / Q000235:genetics* / Q000503:physiopathology; D059467:Transcriptome; D015854:Up-Regulation