title: Decreased glucagon-like peptide-1 correlates with abdominal pain in patients with constipation-predominant irritable bowel syndrome.
abstract: OBJECTIVE
  The glucagon-like peptide-1 (GLP-1) analog, ROSE-010, plays a critical role in alleviating abdominal pain in patients with irritable bowel syndrome (IBS); however, the underling mechanism is unclear. In the present study, we determined the serum GLP-1 level in patients with constipation-predominant IBS (IBS-C). The relationship between GLP-1 and abdominal pain was investigated. In addition, the expression of the GLP-1 receptor in the colon was determined.
  
  
  METHODS
  Rectosigmoid biopsies were gathered from 38 patients with IBS-C who met the Rome III criteria, and 22 healthy controls. Abdominal pain was quantified by a validated questionnaire. Serum GLP-1 was measured by ELISA and correlated with abdominal pain scores. The presence of the GLP-1 receptor in the colonic mucosa was assessed by immunohistochemistry.
  
  
  RESULTS
  Serum GLP-1 was substantially decreased in patients with IBS-C. Decreased serum GLP-1 had a negative correlation with the abdominal pain scores. Biopsies from patients with IBS-C revealed a significant down-regulation of the GLP-1 receptor in colonic mucosa compared with control subjects.
  
  
  CONCLUSIONS
  Decreased serum GLP-1 correlates with abdominal pain in patients with IBS-C. Decreased expression of GLP-1 and GLP-1 receptor may be the basis for alleviation of abdominal pain in patients with IBS-C by ROSE-010.
authors.affiliation: Department of Gastroenterology, Dalian friendship Hospital, Dalian, Liaoning 116001, PR China.
authors.forename: Zheng-Yang
authors.initials: ZY
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, Dalian friendship Hospital, Dalian, Liaoning 116001, PR China.
authors.forename: Na
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, Dalian friendship Hospital, Dalian, Liaoning 116001, PR China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Wen
authors.affiliation: Department of Gastroenterology, Dalian friendship Hospital, Dalian, Liaoning 116001, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, Dalian friendship Hospital, Dalian, Liaoning 116001, PR China.
authors.forename: Xiu-Li
authors.initials: XL
authors.lastname: Sun
authors.affiliation: Department of Gastroenterology, Jinshan hospital of Fudan University, Shanghai 201508, PR China. Electronic address: xiaomingfan57@hotmail.com.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Fan
authors.affiliation: Department of Gastroenterology, Dalian friendship Hospital, Dalian, Liaoning 116001, PR China. Electronic address: yonghongs@163.com.
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Sun
mesh_terms: D015746:Abdominal Pain / Q000097:blood* / Q000209:etiology; D000328:Adult; D000368:Aged; D003248:Constipation / Q000097:blood* / Q000209:etiology; D005260:Female; D052216:Glucagon-Like Peptide 1 / Q000097:blood*; D006801:Humans; D043183:Irritable Bowel Syndrome / Q000097:blood* / Q000150:complications; D008297:Male; D008875:Middle Aged
chemical_list: D052216:Glucagon-Like Peptide 1