title: Electrical stimulation of somatic human stem cells mediated by composite containing conductive nanofibers for ligament regeneration.
abstract: One of the advances in the field of biomedical nanotechnology, is conductive nanofiber fabrication and the discovery of its applications. Biocompatible flexible nanofibers that have a good biocompatibility, mechanical properties and morphology. Poly (3, 4-ethylene dioxythiophene) (PEDOT) is a conductive polymer that has recently been used in medical applications. In this study, the electrospinning technique and vapor phase polymerization combination method with freeze drying was used to produce Silk fibroin/PEDOT/Chitosan nanocomposite scaffold. The aim of our study was to develop a ligament construct of PEDOT/Silk bilayer nanofibrous scaffold, to mimic the aligned collagen fiber bundles and Chitosan sponge coating was done on these fibrous scaffolds, to mimic the glycosaminoglycans of ECM sheath. The developed constructs were characterized. The unrestricted somatic human stem cells (USSC), were cultured on the scaffold. Then, the effect of applying DC electric pulses to cells cultured on polymer was assessed. Cellular function was actively exhibited in scaffold with electrical induction, as evident by the high expression of collagen I, collagen III, decorin, biglycan and aggrecan genes. Novel scaffold plus electrical stimulation shows facilitating cell seeding and promoting cell proliferation, differentiation. This composites can be used in this new field for stem cells differentiation to target tissues.
authors.affiliation: Textile Engineering Department, Amirkabir University of Technology, Tehran, Iran.
authors.forename: Masumeh
authors.initials: M
authors.lastname: Dodel
authors.affiliation: Textile Engineering Department, Amirkabir University of Technology, Tehran, Iran. Electronic address: hemmati@aut.ac.ir.
authors.forename: Nahid
authors.initials: N
authors.lastname: Hemmati Nejad
authors.affiliation: Textile Engineering Department, Amirkabir University of Technology, Tehran, Iran.
authors.forename: S Hajir
authors.initials: SH
authors.lastname: Bahrami
authors.affiliation: Stem Cell Technology Research Center, Tehran, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
authors.affiliation: School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Mohammadi Amirabad
authors.affiliation: Stem Cell Technology Research Center, Tehran, Iran.
authors.forename: Hana
authors.initials: H
authors.lastname: Hanaee-Ahvaz
authors.affiliation: Stem Cell and Tissue Engineering Research Center, Shahroud University of Medical Sciences, Shahroud, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Atashi
mesh_terms: D058578:Biglycan / Q000235:genetics; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry; D024042:Collagen Type I / Q000235:genetics; D024061:Collagen Type III / Q000235:genetics; D058575:Decorin / Q000235:genetics; D004558:Electric Stimulation; D055664:Electrochemical Techniques; D005312:Fetal Blood / Q000166:cytology; D005349:Fibroins / Q000737:chemistry; D015870:Gene Expression; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D008022:Ligaments / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry / Q000648:ultrastructure; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry; D012038:Regeneration; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D058578:Biglycan; D019086:Bridged Bicyclo Compounds, Heterocyclic; D024042:Collagen Type I; D024061:Collagen Type III; D058575:Decorin; D011108:Polymers; C121383:poly(3,4-ethylene dioxythiophene); D005349:Fibroins; D048271:Chitosan
keywords: Bioreactor; Conductive scaffold; Electrical stimulation; Electrospinning; Poly (3, 4-ethylene dioxythiophene); Stem cells