title: Phytochemical Analysis of Agrimonia pilosa Ledeb, Its Antioxidant Activity and Aldose Reductase Inhibitory Potential.
abstract: The aim of this study was to determine aldose reductase (AR) inhibitory activity and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity of compounds fromAgrimonia pilosaLedeb (AP). We isolated agrimoniin (AM), four flavonoid glucosides and two flavonoid glucuronides from then-butanol fraction of AP 50% methanol extract. In addition to isolated compounds, the AR-inhibitory activity and the DPPH free radical scavenging activity of catechin, 5-flavonoids, and 4-flavonoid glucosides (known components of AP) against rat lens AR (RLAR) and DPPH assay were measured. AM showed IC50values of 1.6 and 13.0 μM against RLAR and DPPH scavenging activity, respectively. Additionally, AM, luteolin-7-O-glucuronide (LGN), quercitrin (QU), luteolin (LT) and afzelin (AZ) showed high inhibitory activity against AR and were first observed to decrease sorbitol accumulation in the rat lens under high-sorbitol conditions ex vivo with inhibitory values of 47.6%, 91.8%, 76.9%, 91.8% and 93.2%, respectively. Inhibition of recombinant human AR by AM, LGN and AZ exhibited a noncompetitive inhibition pattern. Based on our results, AP and its constituents may play partial roles in RLAR and oxidative radical inhibition. Our results suggest that AM, LGN, QU, LT and AZ may potentially be used as natural drugs for treating diabetic complications.
authors.affiliation: Department of Food Science and Nutrition, Hallym University, 1 Hallymdaehak-gil, Chuncheon, Gangwon-do 24252, Korea. jwsbcb0187@naver.com.
authors.forename: Set Byeol
authors.initials: SB
authors.lastname: Kim
authors.affiliation: Department of Food Science and Nutrition, Hallym University, 1 Hallymdaehak-gil, Chuncheon, Gangwon-do 24252, Korea. isohsh@gmail.com.
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Hwang
authors.affiliation: Institute of Natural Medicine, College of Medicine, Hallym University, 1 Hallymdaehak-gil, Chuncheon, Gangwon-do 24252, Korea. hwsuh@hallym.ac.kr.
authors.forename: Hong-Won
authors.initials: HW
authors.lastname: Suh
authors.affiliation: Department of Food Science and Nutrition, Hallym University, 1 Hallymdaehak-gil, Chuncheon, Gangwon-do 24252, Korea. limss@hallym.ac.kr.|Institute of Natural Medicine, College of Medicine, Hallym University, 1 Hallymdaehak-gil, Chuncheon, Gangwon-do 24252, Korea. limss@hallym.ac.kr.
authors.forename: Soon Sung
authors.initials: SS
authors.lastname: Lim
mesh_terms: D031981:Agrimonia / Q000737:chemistry*; D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors*; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004789:Enzyme Activation; D005419:Flavonoids / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D007700:Kinetics; D015394:Molecular Structure; D064209:Phytochemicals / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D016166:Free Radical Scavengers; D064209:Phytochemicals; D010936:Plant Extracts; D000449:Aldehyde Reductase
keywords: 1,1-diphenyl-2-picrylhydrazyl (DPPH); Agrimonia pilosa Ledeb; aldose reductase; diabetic complication; flavonoids