title: Pharmacological evaluation ofApium leptophyllum(Pers.) on bronchial asthma.
abstract: OBJECTIVE
  The present study was to investigate the antiasthmatic potential from the flavonoid fraction ofApium leptophyllumfruit (FFALF) to validate its traditional claim.
  
  
  METHODS
  The antiasthmatic activity of FFALF was evaluated by histamine or acetylcholine-induced bronchospasm model in guinea pigs, compound 48/80 induced mast cell degranulation in albino rats and histamine-induced tracheal contraction in guinea pig. The preconvulsion dyspnea time at 0thand 7thday at the dose of 100 and 200 mg/kg in guinea pig's bronchospasm model, the percentage of granulated and degranulated mast cell at the dose of 500, 750, and 1000 μg/ml in rats and tracheal contraction at the dose of 500, 750, and 1000 μg/ml in guinea pig were measured and compared with respective control groups.
  
  
  RESULTS
  The treatments of FFALF were significantly (P< 0.001) decreased the histamine/acetylcholine-induced bronchospasm, mast cell degranulation, and histamine-induced tracheal contraction as compared to inducer group. In addition, FFALF showed dose-dependent antiasthmatic activity in all the animals.
  
  
  CONCLUSIONS
  Hence, this study suggested that the FFALF showed antiasthmatic activity probably by membrane stabilizing property as well as suppressing antibody production and inhibiting of antigen induced by histamine and acetylcholine.
authors.affiliation: Department of Pharmacology and Experimental Biology, Vedica College of Pharmacy, RKDF University, Bhopal, Madhya Pradesh, India.
authors.forename: Himanshu Bhusan
authors.initials: HB
authors.lastname: Sahoo
authors.affiliation: Department of Pharmacology and Experimental Biology, Vedica College of Pharmacy, RKDF University, Bhopal, Madhya Pradesh, India.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Sagar
authors.affiliation: Department of Pharmacology, College of Pharmaceutical Sciences, Mohuda, Berhampur, Odisha, India.
authors.forename: Ganesh
authors.initials: G
authors.lastname: Patro
authors.affiliation: Department of Pharmacology, College of Pharmaceutical Sciences, Mohuda, Berhampur, Odisha, India.
authors.forename: Madhulita
authors.initials: M
authors.lastname: Panda
authors.affiliation: Department of Pharmaceutical Chemistry, Sri Sivani College of Pharmacy, Srikakulam, Andhra Pradesh, India.
authors.forename: Saroj Kumar
authors.initials: SK
authors.lastname: Sahoo
authors.affiliation: Department of Pharmaceutical Chemistry, Sri Sivani College of Pharmacy, Srikakulam, Andhra Pradesh, India.
authors.forename: D
authors.initials: D
authors.lastname: Priyadarshini
keywords: Apium leptophyllum; asthma; bronchospasm; histamine; mast cell degranulation