title: Cloning and expression of cry2Aa from native Bacillus thuringiensis strain SY49-1 and its insecticidal activity against Culex pipiens (Diptera: Culicidae).
abstract: Bacillus thuringiensis (Berliner) (Bt) is well known for having toxicity against pest insects because of their ability to form endospores and broad-range activity of their parasporal inclusions. In this study, a new member of cry2A gene from previously characterized native B. thuringiensis SY49-1 strain was cloned, expressed and used for its activity against Culex pipiens (Diptera: Culicidae) larvae. The sequence analysis of the cloned cry2A gene revealed that it encodes a polypeptide of 633 aa residues with 99% identity to Cry2Aa protein with expected molecular weight of 70.7 kDa. Bacillus thuringiensis delta-endotoxin nomenclature committee designed our sequence as Cry2Aa18 being a new member of Bt toxins. Bioassays against last instar larvae of C. pipiens indicated that Cry2Aa18 has considerable toxicity with LC50of 630 μg ml-1. In order to prevent the spread of infectious diseases mediated by C. pipiens, this newly characterized cry2Aa18 gene could constitute as an important biological control tool for controlling mosquito larvae living in freshwater systems and can be used as a good alternative for minimizing the use of chemicals.
authors.affiliation: Department of Agricultural Biotechnology, Erciyes University, Kayseri, Turkey. Electronic address: ylmazsemh@yahoo.com.
authors.forename: Semih
authors.initials: S
authors.lastname: Yilmaz
authors.affiliation: Department of Crop and Animal Production, Erciyes University, Tomarza Mustafa Akıncıoğlu Vocational College, Kayseri, Turkey. Electronic address: azizoglu@erciyes.edu.tr.
authors.forename: Ugur
authors.initials: U
authors.lastname: Azizoglu
authors.affiliation: Department of Biology, Faculty of Science, Erciyes University, Kayseri, Turkey. Electronic address: ayvaza@erciyes.edu.tr.
authors.forename: Abdurrahman
authors.initials: A
authors.lastname: Ayvaz
authors.affiliation: Department of Biology, Erciyes University, Kayseri, Turkey. Electronic address: rtemizgul@erciyes.edu.tr.
authors.forename: Ridvan
authors.initials: R
authors.lastname: Temizgul
authors.affiliation: Graduate School of Natural and Applied Sciences, Erciyes University, Kayseri, Turkey. Electronic address: atczehra58@gmail.com.
authors.forename: Zehra Büşra
authors.initials: ZB
authors.lastname: Atciyurt
authors.affiliation: Department of Field Crops, Faculty of Agriculture and Natural Science, Düzce University, Düzce, Turkey. Electronic address: salihkaraborklu@duzce.edu.tr.
authors.forename: Salih
authors.initials: S
authors.lastname: Karabörklü
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000633:toxicity*; D001681:Biological Assay; D003001:Cloning, Molecular; D003465:Culex / Q000187:drug effects* / Q000502:physiology*; D004731:Endotoxins / Q000737:chemistry / Q000235:genetics* / Q000633:toxicity*; D015870:Gene Expression; D006460:Hemolysin Proteins / Q000737:chemistry / Q000235:genetics* / Q000633:toxicity*; D007306:Insecticides / Q000737:chemistry / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000502:physiology; D008970:Molecular Weight; D017386:Sequence Homology, Amino Acid
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis
keywords: Bacillus thuringiensis; Bt SY49-1; Cloning; Culex pipiens; Larvicidal activity; cry2Aa18