title: Circulation of multiple subtypes of bovine viral diarrhoea virus type 1 with no evidence for HoBi-like pestivirus in cattle herds of southern Italy.
abstract: Pestiviruses of cattle include bovine viral diarrhoea 1 (BVDV-1) and 2 (BVDV-2) plus an emerging group, named HoBi-like pestivirus. In the present paper, the results of an epidemiological survey for pestiviruses circulating in cattle in southern Italy are presented. Molecular assays carried out on a total of 924 bovine samples detected 74 BVDV strains, including 73 BVDV-1 and 1 BVDV-2 viruses. Phylogenetic analysis carried out on partial 5'UTR and Nprosequences revealed the presence of 6 different subtypes of BVDV-1 and a single BVDV-2c strain. BVDV-1 displayed a high level of genetic heterogeneity, which can have both prophylactic and diagnostic implications. In addition, the detection of BVDV-2c highlights the need for a continuous surveillance for the emergence of new pestivirus strains in cattle farms in southern Italy.
authors.affiliation: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Lanave
authors.affiliation: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy. Electronic address: nicola.decaro@uniba.it.
authors.forename: N
authors.initials: N
authors.lastname: Decaro
authors.affiliation: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
authors.forename: M S
authors.initials: MS
authors.lastname: Lucente
authors.affiliation: Istituto Zooprofilattico Sperimentale della Sicilia, Palermo, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Guercio
authors.affiliation: Istituto Zooprofilattico Sperimentale di Puglia e Basilicata, Foggia, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Cavaliere
authors.affiliation: Istituto Zooprofilattico Sperimentale della Sicilia, Palermo, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Purpari
authors.affiliation: Istituto Zooprofilattico Sperimentale di Puglia e Basilicata, Foggia, Italy.
authors.forename: I
authors.initials: I
authors.lastname: Padalino
authors.affiliation: Istituto Zooprofilattico Sperimentale di Puglia e Basilicata, Foggia, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Larocca
authors.affiliation: Istituto Zooprofilattico Sperimentale della Sicilia, Palermo, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Antoci
authors.affiliation: Istituto Zooprofilattico Sperimentale di Puglia e Basilicata, Foggia, Italy.
authors.forename: P A
authors.initials: PA
authors.lastname: Marino
authors.affiliation: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Buonavoglia
authors.affiliation: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Elia
mesh_terms: D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000453:epidemiology* / Q000473:pathology / Q000635:transmission / Q000821:virology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000473:pathology / Q000635:transmission / Q000821:virology; D030241:Diarrhea Virus 1, Bovine Viral / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D030242:Diarrhea Virus 2, Bovine Viral / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D062665:Epidemiological Monitoring; D005260:Female; D018740:Genetic Heterogeneity; D007558:Italy / Q000453:epidemiology; D008168:Lung / Q000473:pathology / Q000821:virology; D010576:Pestivirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D010920:Placenta / Q000473:pathology / Q000821:virology; D011247:Pregnancy; D013154:Spleen / Q000473:pathology / Q000821:virology
keywords: BVDV; Cattle; Pestivirus; Subtypes