title: Nano Endoscopy with Plasmon-Enhanced Fluorescence for Sensitive Sensing Inside Ultrasmall Volume Samples.
abstract: Plasmon-enhanced fluorescence (PEF) generally requires the samples settled on a metal substrate and the effective enhancement distance is less than 100 nm, which limit its application in intracellular sensing. Herein, we report a nano endoscopy with PEF effect for sensing analytes inside the extremely small volume samples. The nano endoscopy was fabricated by assembling single nanoporous gold nanowire (PGNW) on the tip of a tungsten needle. It was accurately manipulated to insert into a micro droplet, and an effective sensing was realized at micrometre scale with submicrometer resolution. By taking lysozyme as a model sensing target, a 23-fold improvement of sensitivity was obtained, comparing with that of smooth gold nanowire (SGNW). These results indicated that the nano endoscopy can realize a high spatial resolution sensing, showing its potential application in intracellular sensing.
authors.affiliation: Institute of Nuclear and New Energy Technology, Collaborative Innovation Center of Advanced Nuclear Energy Technology, Tsinghua University , Beijing 100084, P. R. China.
authors.forename: Hang
authors.initials: H
authors.lastname: Yuan
authors.affiliation: Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Tsinghua University , Beijing 100084, P. R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Nuclear and New Energy Technology, Collaborative Innovation Center of Advanced Nuclear Energy Technology, Tsinghua University , Beijing 100084, P. R. China.
authors.forename: Yuexiang
authors.identifier: 0000-0003-2755-7733
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Institute of Nuclear and New Energy Technology, Collaborative Innovation Center of Advanced Nuclear Energy Technology, Tsinghua University , Beijing 100084, P. R. China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Institute of Nuclear and New Energy Technology, Collaborative Innovation Center of Advanced Nuclear Energy Technology, Tsinghua University , Beijing 100084, P. R. China.
authors.forename: Guoyu
authors.initials: G
authors.lastname: Wei
authors.affiliation: Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Tsinghua University , Beijing 100084, P. R. China.
authors.forename: Tianhao
authors.initials: T
authors.lastname: Wu
authors.forename: Gang
authors.identifier: 0000-0002-7066-940X
authors.initials: G
authors.lastname: Ye
authors.affiliation: Institute of Nuclear and New Energy Technology, Collaborative Innovation Center of Advanced Nuclear Energy Technology, Tsinghua University , Beijing 100084, P. R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Tsinghua University , Beijing 100084, P. R. China.
authors.forename: Sichun
authors.identifier: 0000-0001-8927-2376
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Tsinghua University , Beijing 100084, P. R. China.
authors.forename: Xinrong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D002232:Carbocyanines / Q000737:chemistry; D002645:Chickens; D004724:Endoscopy / Q000295:instrumentation*; D004867:Equipment Design; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D009113:Muramidase / Q000032:analysis*; D053770:Nanowires / Q000737:chemistry*; D016062:Porosity; D020349:Surface Plasmon Resonance / Q000295:instrumentation*
chemical_list: D052157:Aptamers, Nucleotide; D002232:Carbocyanines; D005456:Fluorescent Dyes; C085321:cyanine dye 5; D006046:Gold; D009113:Muramidase