title: Nipped-A regulates intestinal stem cell proliferation inDrosophila.
abstract: Adult stem cells uphold a delicate balance between quiescent and active states, a deregulation of which can lead to age-associated diseases such as cancer. InDrosophila, intestinal stem cell (ISC) proliferation is tightly regulated and mis-regulation is detrimental to intestinal homeostasis. Various factors are known to govern ISC behavior; however, transcriptional changes in ISCs during aging are still unclear. RNA sequencing of young and old ISCs newly identified Nipped-A, a subunit of histone acetyltransferase complexes, as a regulator of ISC proliferation that is upregulated in old ISCs. We show that Nipped-A is required for maintaining the proliferative capacity of ISCs during aging and in response to tissue-damaging or tumorigenic stimuli. Interestingly,DrosophilaMyc cannot compensate for the effect of the loss of Nipped-A on ISC proliferation. Nipped-A seems to be a superordinate regulator of ISC proliferation, possibly by coordinating different processes including modifying the chromatin landscape of ISCs and progenitors.
authors.affiliation: Institut für Biochemie und Molekulare Biologie, Universität Ulm, Albert-Einstein-Allee 11, Ulm 89081, Germany hmtauc@gmail.com petra.pandur@uni-ulm.de.
authors.forename: Helen Marie
authors.initials: HM
authors.lastname: Tauc
authors.affiliation: Institut für Immunologie, Universitätsklinikum Ulm, Albert-Einstein-Allee 11, Ulm 89081, Germany.
authors.forename: Alpaslan
authors.initials: A
authors.lastname: Tasdogan
authors.affiliation: Institut für Dermatologie, Universität Ulm, Life Science Building N27, James Franck-Ring/Meyerhofstrasse 11c, Ulm 89081, Germany.
authors.forename: Patrick
authors.initials: P
authors.lastname: Meyer
authors.affiliation: Institut für Biochemie und Molekulare Biologie, Universität Ulm, Albert-Einstein-Allee 11, Ulm 89081, Germany hmtauc@gmail.com petra.pandur@uni-ulm.de.
authors.forename: Petra
authors.identifier: 0000-0001-8973-5211
authors.initials: P
authors.lastname: Pandur
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology*; D000375:Aging; D000818:Animals; D002453:Cell Cycle; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation; D002843:Chromatin / Q000378:metabolism; D029721:Drosophila Proteins / Q000502:physiology*; D004331:Drosophila melanogaster / Q000502:physiology*; D005434:Flow Cytometry; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D006706:Homeostasis; D007422:Intestines / Q000166:cytology*; D010641:Phenotype; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D034622:RNA Interference; D017423:Sequence Analysis, RNA; D015398:Signal Transduction; D014157:Transcription Factors / Q000502:physiology*
chemical_list: D002843:Chromatin; D029721:Drosophila Proteins; D006657:Histones; C511055:NIPPED-A protein, Drosophila; D016271:Proto-Oncogene Proteins c-myc; D014157:Transcription Factors; D049452:Green Fluorescent Proteins
keywords: Aging; Chromatin; Intestine; Myc; Stem cell; TRRAP