title: MoSe2Nanolabels for Electrochemical Immunoassays.
abstract: There is huge interest in biosensors as a result of the demand for personalized medicine. In biomolecular detection, transition-metal dichalcogenides (TMDs) can be used as signal-enhancing elements. Herein, we utilize a solution-based electrochemical exfoliation technique with bipolar electrodes to manufacture MoSe2nanolabels for biomolecular detection. Prepared MoSe2nanoparticles (NPs) exhibit electrocatalytic activity toward the hydrogen evolution reaction (HER), and such a property allows it to act as a robust label for magneto-immunoassays toward protein detection. The magneto-immunoassay also displayed good selectivity, a wide linear range of 2 to 500 ng mL-1, high sensitivity (LOD = 1.23 ng mL-1) and reproducibility (RSD = 9.7%). These findings establish the viability and reproducibility of such an exfoliation technique for TMD nanolabels for the development of low costs and efficient biosensing systems.
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University , Singapore 637371.
authors.forename: Rou Jun
authors.initials: RJ
authors.lastname: Toh
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University , Singapore 637371.
authors.forename: Carmen C
authors.initials: CC
authors.lastname: Mayorga-Martinez
authors.affiliation: Department of Inorganic Chemistry, University of Chemistry and Technology Prague , Technicka 5, 166 28 Prague 6, Czech Republic.
authors.forename: Zdeněk
authors.initials: Z
authors.lastname: Sofer
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University , Singapore 637371.
authors.forename: Martin
authors.initials: M
authors.lastname: Pumera
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D006041:Goats; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood*; D057230:Limit of Detection; D008982:Molybdenum / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011817:Rabbits; D015203:Reproducibility of Results; D012643:Selenium / Q000737:chemistry*
chemical_list: D007074:Immunoglobulin G; D008982:Molybdenum; D012643:Selenium