title: Inhibition of Amyloid-like Fibril Formation of Trypsin by Red Wines.
abstract: The aim of the present study was to examine the potential role and applicability of dietary supplements in reducing the risk of development of amyloid diseases associated with the gastrointestinal tract, such as type II diabetes. Trypsin, a well-known serine protease was used as a model protein in our experiments. The effect of various red wines on the formation of amyloid-like fibrils of trypsin was studied in vitro, in aqueous ethanol, at pH 7.0. Turbidity measurements, aggregation kinetics experiments, Congo red binding assays and electronic circular dichroism spectroscopic measurements were used to follow the aggregation process in the presence or absence of various red wines. The results suggest that red wines effectively inhibit the formation of amyloid-like fibrils of trypsin and the inhibitory effect is dose-dependent. The extent of inhibition was found to be proportional to the total concentration of phenolic compounds.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, Középfasor 52. Hungary.
authors.forename: Marta
authors.initials: M
authors.lastname: Kotorman
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, H-6726 Szeged. Hungary.
authors.forename: Phanindra B
authors.initials: PB
authors.lastname: Kasi
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, H-6726 Szeged. Hungary.
authors.forename: Laszlo
authors.initials: L
authors.lastname: Halasz
authors.affiliation: Laboratory of Chemical Biology, Biological Research Centre of Hungarian Academy of Sciences, Temesvári krt. 62, H-6726 Szeged. Hungary.
authors.forename: Attila
authors.initials: A
authors.lastname: Borics
mesh_terms: D000682:Amyloid / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D003224:Congo Red; D007700:Kinetics; D010636:Phenols / Q000494:pharmacology; D014357:Trypsin / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D014920:Wine
chemical_list: D000682:Amyloid; D010636:Phenols; D003224:Congo Red; D014357:Trypsin
keywords: Amyloid-like fibrils; congo red; electronic circular dichroism; natural polyphenols; red wine; trypsin