title: Changes of membrane fatty acids and proteins ofShewanella putrefacienstreated with cinnamon oil and gamma irradiation.
abstract: BACKGROUND
  In order to detect the antimicrobial mechanism of combined treatment of cinnamon oil and gamma irradiation (GI), the membrane fatty acids and proteins characteristics ofShewanella putrefaciens(S. putrefaciens) treated with cinnamon oil and GI, and the distribution of cinnamon oil inS. putrefacienswere observed in this study.
  
  
  RESULTS
  The membrane lipid profile ofS. putrefacienswas notably damaged by treatments of cinnamon oil and the combination of cinnamon oil and GI, with significantly fatty acids decrease in C14:0, C16:0, C16:1, C17:1, C18:1 (p< 0.05). The SDS-PAGE result showed that GI did not have obvious effect on membrane proteins (MP), but GI combined with cinnamon oil changed the MP subunits. Cinnamaldehyde, the main component of cinnamon oil, can not transport intoS. putrefaciensobviously. It was transformed into cinnamyl alcohol in the nutrient broth with the action ofS. putrefaciens. This indicated that the antimicrobial action of cinnamon oil mainly happened on the membrane ofS. putrefaciens.
  
  
  CONCLUSIONS
  Cinnamon oil could act on the membrane ofS. putrefacienswith the damage of fatty acids and proteins, and GI would increase the destructive capability of cinnamon oil on the membrane fatty acids and proteins ofS. putrefaciens.
authors.affiliation: Department of Food Science, Ocean College, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014 China.
authors.forename: Fei
authors.initials: F
authors.lastname: Lyu
authors.affiliation: Department of Food Science, Ocean College, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014 China.
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.affiliation: Department of Food Science, Ocean College, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014 China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Wei
authors.affiliation: Department of Food Science, Ocean College, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014 China.
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
keywords: Cinnamon oil; Fatty acids; Gamma irradiation; Shewanella putrefaciens