title: Genetic mapping and QTL analysis of Botrytis resistance inGerbera hybrida.
abstract: Gerbera hybridais an economically important cut flower. In the production and transportation of gerbera with unavoidable periods of high relative humidity, grey mould occurs and results in losses in quality and quantity of flowers. Considering the limitations of chemical use in greenhouses and the impossibility to use these chemicals in auction or after sale, breeding for resistant gerbera cultivars is considered as the best practical approach. In this study, we developed two segregating F1 populations (called S and F). Four parental linkage maps were constructed using common and parental specific SNP markers developed from expressed sequence tag sequencing. Parental genetic maps, containing 30, 29, 27 and 28 linkage groups and a consensus map covering 24 of the 25 expected chromosomes, could be constructed. After evaluation ofBotrytisdisease severity using three different tests,whole inflorescence,bottom(of disc florets) andray floret, quantitative trait locus (QTL) mapping was performed using the four individual parental maps. A total of 20 QTLs (including one identical QTL for whole inflorescence and bottom tests) were identified in the parental maps of the two populations. The number of QTLs found and the explained variance of most QTLs detected reflect the complex mechanism ofBotrytisdisease response.
authors.affiliation: Plant Breeding, Wageningen University & Research, PO Box 386, 6700AJ Wageningen, The Netherlands.
authors.forename: Yiqian
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Plant Breeding, Wageningen University & Research, PO Box 386, 6700AJ Wageningen, The Netherlands.
authors.forename: Alex
authors.initials: A
authors.lastname: van Silfhout
authors.affiliation: Plant Breeding, Wageningen University & Research, PO Box 386, 6700AJ Wageningen, The Netherlands.
authors.forename: Arwa
authors.initials: A
authors.lastname: Shahin
authors.affiliation: Schreurs Holland B.V., Hoofdweg 81, 1424PD De Kwakel, The Netherlands.
authors.forename: Ronny
authors.initials: R
authors.lastname: Egberts
authors.affiliation: Florist Holland B.V., Dwarsweg 15, 1424PL De Kwakel, The Netherlands.
authors.forename: Martin
authors.initials: M
authors.lastname: Beers
authors.affiliation: Florist Holland B.V., Dwarsweg 15, 1424PL De Kwakel, The Netherlands.
authors.forename: Ans
authors.initials: A
authors.lastname: van der Velde
authors.affiliation: Schreurs Holland B.V., Hoofdweg 81, 1424PD De Kwakel, The Netherlands.
authors.forename: Adrie
authors.initials: A
authors.lastname: van Houten
authors.affiliation: Plant Breeding, Wageningen University & Research, PO Box 386, 6700AJ Wageningen, The Netherlands.
authors.forename: Jaap M
authors.initials: JM
authors.lastname: van Tuyl
authors.affiliation: Plant Breeding, Wageningen University & Research, PO Box 386, 6700AJ Wageningen, The Netherlands.
authors.forename: Richard G F
authors.initials: RGF
authors.lastname: Visser
authors.affiliation: Plant Breeding, Wageningen University & Research, PO Box 386, 6700AJ Wageningen, The Netherlands.
authors.forename: Paul
authors.identifier: 0000-0003-2118-389X
authors.initials: P
authors.lastname: Arens
keywords: Gerbera grey mould; Linkage group; QTL mapping; SNP