title: Zunyimycins B and C, New Chloroanthrabenzoxocinones Antibiotics against Methicillin-Resistant Staphylococcus aureus and Enterococci from Streptomyces sp. FJS31-2.
abstract: This study performed an optimization of the fermentation conditions to activate the expression of the zunyimycin family biosynthesis genes of the zunyimycin-producing streptomycetes strainStreptomycessp. FJS31-2. Bioassay-guided isolation and purification by varied chromatographic methods yielded two new compounds of the zunyimycin derivatives, namely, 31-2-7 and 31-2-8, accompanied with three known anthrabenzoxocinones family members of zunyimycin A, BE24566B, and chloroanthrabenzoxocinone. Their structures were elucidated by NMR, HRESIMS, IR, UV, and CD. Results showed that these two compounds were structurally similar to the previously reported compound zunyimycin A but differed in positions and number of chlorine atom substitution. The two novel compounds were called zunyimycins B and C. Antibacterial activity assay indicated that zunyimycin C showed a good inhibitory effect on the methicillin-resistantStaphylococcus aureusandEnterococci.
authors.affiliation: Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi 563003, Guizhou, China. 18585737637@163.com.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Lü
authors.affiliation: Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi 563003, Guizhou, China. meiyun216@126.com.
authors.forename: Meiyun
authors.initials: M
authors.lastname: Shao
authors.affiliation: Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi 563003, Guizhou, China. yinyinwang0530@126.com.
authors.forename: Yinyin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi 563003, Guizhou, China. qianshengyan@126.com.
authors.forename: Shengyan
authors.initials: S
authors.lastname: Qian
authors.affiliation: Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi 563003, Guizhou, China. wangmiao_good@163.com.
authors.forename: Miao
authors.initials: M
authors.lastname: Wang
authors.affiliation: Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi 563003, Guizhou, China. yingquanwang@126.com.
authors.forename: Yingquan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi 563003, Guizhou, China. xiaoqianli0908@163.com.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Li
authors.affiliation: Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi 563003, Guizhou, China. baoyuxin8680915@126.com.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Bao
authors.affiliation: Zunyi Key Laboratory of Genetic Diagnosis & Targeted Drug Therapy, The First People's Hospital of Zunyi, Zunyi 563003, Guizhou, China. dengcmin@126.com.
authors.forename: Chengmin
authors.initials: C
authors.lastname: Deng
authors.affiliation: Zunyi Key Laboratory of Genetic Diagnosis & Targeted Drug Therapy, The First People's Hospital of Zunyi, Zunyi 563003, Guizhou, China. changwuyue@hotmail.com.
authors.forename: Changwu
authors.initials: C
authors.lastname: Yue
authors.affiliation: Zunyi Key Laboratory of Genetic Diagnosis & Targeted Drug Therapy, The First People's Hospital of Zunyi, Zunyi 563003, Guizhou, China. ldsdoc@126.com.
authors.forename: Daishun
authors.initials: D
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. fussliu@126.com.
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. lysf1987313@163.com.
authors.forename: Minghao
authors.initials: M
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. huangy@im.ac.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Clinical Laboratory Medicine, Zunyi Medical University, Zunyi 563003, Guizhou, China. czhtyb@163.com.
authors.forename: Zehui
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Clinical Laboratory Medicine, Zunyi Medical University, Zunyi 563003, Guizhou, China. huyonglin5559@163.com.
authors.forename: Yonglin
authors.initials: Y
authors.lastname: Hu
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000737:chemistry* / Q000494:pharmacology*; D002942:Circular Dichroism; D016983:Enterococcus / Q000187:drug effects*; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents
keywords: Enterococci; MRSA; activity; antibacterial; chloroanthrabenzoxocinones; streptomycetes; zunyimycins