title: Biphasic organo-bioceramic fibrous composite as a biomimetic extracellular matrix for bone tissue regeneration.
abstract: In bone tissue engineering, the organo-ceramic composite, electrospun polycaprolactone/hydroxyapatite (PCL/HA) scaffold has the potential to support cell proliferation, migration, differentiation, and homeostasis. Here, we report the effect of PCL/HA scaffold in tissue regeneration using human mesenchymal stem cells (hMSCs). We characterized the scaffold by fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), scanning electron microscope (SEM) and assessed its biocompatibility. PCL/HA composite is superior as a scaffold compared to PCL alone. Furthermore, increasing HA content (5-10%) was more efficacious in supporting cell-scaffold attachment, expression of ECM molecules and proliferation. These results suggest that PCL/HA is useful as a scaffold for tissue regeneration.
authors.affiliation: Cancer Biology Research and Training Program, Department of Biological Sciences ,Alabama State University, Montgomery, AL 36104, USA.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Cancer Biology Research and Training Program, Department of Biological Sciences,Alabama State University, Montgomery, AL 36104, USA.
authors.forename: James A
authors.initials: JA
authors.lastname: Stokes
authors.affiliation: Biomedical Engineering, Alabama State University, Montgomery, AL 36104, USA.
authors.forename: Derrick
authors.initials: D
authors.lastname: Dean
authors.affiliation: Physical Sciences, Alabama State University, Montgomery, AL 36104, USA.
authors.forename: Christian
authors.initials: C
authors.lastname: Rogers
authors.affiliation: Physical Sciences, Alabama State University, Montgomery, AL 36104, USA.
authors.forename: Elijah
authors.initials: E
authors.lastname: Nyairo
authors.affiliation: Department of Material Science and Engineering, University of Alabama, Birmingham, AL USA.
authors.forename: Vinoy
authors.initials: V
authors.lastname: Thomas
authors.affiliation: Cancer Biology Research and Training Program, Department of Biological Sciences, Alabama State University, 915 S Jackson Street, Montgomery, AL 36104, mmishra@alasu.edu.
authors.forename: Manoj K
authors.initials: MK
authors.lastname: Mishra
mesh_terms: D040761:Biomimetic Materials; D001861:Bone Regeneration; D001842:Bone and Bones / Q000166:cytology*; D002152:Calorimetry, Differential Scanning; D017886:Durapatite; D005109:Extracellular Matrix; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology; D008855:Microscopy, Electron, Scanning; D011091:Polyesters; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D011091:Polyesters; C016240:polycaprolactone; D017886:Durapatite