title: Design and evaluation of antibiotic releasing self- assembled scaffolds at room temperature using biodegradable polymer particles.
abstract: Biodegradable polymer-based drug-eluting implants offer many advantages such as predictable drug release kinetics, safety, and acceptable drug loading under ambient conditions. Herein, we describe fabrication and evaluation of antibiotic loaded scaffolds for localized delivery and tissue engineering applications. PDLLA particles entrapping gentamycin were formulated using solvent evaporation method and used for scaffold fabrication. Optimization of formulation parameters such as pH of the internal aqueous phase and combination of excipients like glycerol, polyvinyl alcohol (PVA) resulted in high entrapment efficiencies up to 96% of gentamicin in particles with drug load of 16-18μg/mg of polymer particles. These microparticles were fused in presence of methanol at ambient temperatures to form scaffolds of different geometry having reasonable mechanical strength. Porosity of these scaffolds was found to be more than 80%. Antibiotic released from the scaffolds was found to be bioactive as tested against Staphylococcus aureus and the release pattern was biphasic over a period of one week. The scaffolds were found to be non-toxic to murine fibroblasts cultures in vitro as well as to mice upon subcutaneous implantation. This method provides a novel and easy way of fabricating antibiotic loaded polymer scaffolds for varieties of applications.
authors.affiliation: Product Development Cell, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110067, India. Electronic address: prasad.admane@gmail.com.
authors.forename: Prasad
authors.initials: P
authors.lastname: Admane
authors.affiliation: Product Development Cell, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110067, India. Electronic address: jatingupta35@gmail.com.
authors.forename: Jatin
authors.initials: J
authors.lastname: Gupta
authors.affiliation: Product Development Cell, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110067, India. Electronic address: ancyijpharm@gmail.com.
authors.forename: Ancy
authors.initials: A
authors.lastname: I J
authors.affiliation: Product Development Cell, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110067, India. Electronic address: robin@nii.ac.in.
authors.forename: Robin
authors.initials: R
authors.lastname: Kumar
authors.affiliation: Product Development Cell, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110067, India. Electronic address: amulya@nii.res.in.
authors.forename: Amulya K
authors.initials: AK
authors.lastname: Panda
mesh_terms: D020341:Absorbable Implants / Q000009:adverse effects; D000818:Animals; D063566:Biodegradable Plastics / Q000009:adverse effects / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004343:Drug Implants / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D065546:Drug Liberation; D005347:Fibroblasts / Q000187:drug effects; D005839:Gentamicins / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D005990:Glycerol / Q000737:chemistry; D019344:Lactic Acid / Q000009:adverse effects / Q000737:chemistry*; D051379:Mice; D008826:Microbial Sensitivity Tests; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry; D011100:Polyglycolic Acid / Q000009:adverse effects / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011142:Polyvinyl Alcohol / Q000737:chemistry; D013696:Temperature; D054457:Tissue Scaffolds / Q000009:adverse effects / Q000737:chemistry*
chemical_list: D063566:Biodegradable Plastics; D004343:Drug Implants; D005839:Gentamicins; D011091:Polyesters; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide); D011142:Polyvinyl Alcohol; D005990:Glycerol
keywords: Gentamicin; In vitro release; Microparticles; PDLLA; Scaffolds