title: The boosted biomass and lipid accumulation in Chlorella vulgaris by supplementation of synthetic phytohormone analogs.
abstract: This study attempted at maximizing biomass and lipid accumulation in Chlorella vulgaris by supplementation of natural abscisic acid (ABA) or synthetic 2,4-dichlorophenoxyacetic acid (2,4-D) and 1-naphthaleneacetic acid (NAA) hormone analogs. Amongst three tested additives, NAA-treatment performed remarkable promoting effect on cell growth and lipid biosynthesis. The favorable lipid productivity (418.6mg/L/d) of NAA-treated cells showed 1.48 and 2.24 times more than that of 2,4-D and ABA. NAA-treatment also positively modified the proportions of saturated (C16:0 and C18:0) and monounsaturated fatty acids (C18:1) which were prone to high-quality biofuels-making. Further, NAA-treatment manipulated endogenous phytohormones metabolism leading to the elevated levels of indole-3-acetic acid, jasmonic acid, and salicylic acid and such hormone accumulation might be indispensable for signal transduction in regulating cell growth and lipid biosynthesis in microalgae. In addition, the economic-feasibility and eco-friendly estimation of NAA additive indicated the higher possibilities in developing affordable and scalable microalgal lipids for biofuels.
authors.affiliation: Biological Engineering Department, School of Chemical Engineering, Xiangtan University, Xiangtan, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Liu
authors.affiliation: Biological Engineering Department, School of Chemical Engineering, Xiangtan University, Xiangtan, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: Biological Engineering Department, School of Chemical Engineering, Xiangtan University, Xiangtan, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Biological Engineering Department, School of Chemical Engineering, Xiangtan University, Xiangtan, China.
authors.forename: Zhenyao
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Biological Engineering Department, School of Chemical Engineering, Xiangtan University, Xiangtan, China. Electronic address: yuqinli2004@126.com.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Li
mesh_terms: D018533:Biomass; D053898:Biosynthetic Pathways / Q000187:drug effects / Q000235:genetics; D048408:Chlorella vulgaris / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D005229:Fatty Acids, Monounsaturated / Q000032:analysis; D005240:Feasibility Studies; D005786:Gene Expression Regulation / Q000187:drug effects; D050356:Lipid Metabolism / Q000187:drug effects*; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology*
chemical_list: D005229:Fatty Acids, Monounsaturated; D009280:Naphthaleneacetic Acids; D010937:Plant Growth Regulators; C034182:1-naphthaleneacetic acid
keywords: 1-Naphthaleneacetic acid (NAA); Chlorella vulgaris; Economic and ecological estimation; Endogenous hormones; Lipid