title: Rapid detection of Enterovirus and Coxsackievirus A10 by a TaqMan based duplex one-step real time RT-PCR assay.
abstract: A TaqMan based duplex one-step real time RT-PCR (rRT-PCR) assay was developed for the rapid detection of Coxsackievirus A10 (CV-A10) and other enterovirus (EVs) in clinical samples. The assay was fully evaluated and found to be specific and sensitive. When applied in 115 clinical samples, a 100% diagnostic sensitivity in CV-A10 detection and 97.4% diagnostic sensitivity in other EVs were found.
authors.affiliation: Laboratory of Microbiology, Changsha Center for Disease Control and Prevention, Changsha 410008, China.
authors.forename: Jingfang
authors.initials: J
authors.lastname: Chen
authors.affiliation: Laboratory of Microbiology, Changsha Center for Disease Control and Prevention, Changsha 410008, China.
authors.forename: Rusheng
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Laboratory of Microbiology, Changsha Center for Disease Control and Prevention, Changsha 410008, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Ou
authors.affiliation: Laboratory of Microbiology, Changsha Center for Disease Control and Prevention, Changsha 410008, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Yao
authors.affiliation: Laboratory of Microbiology, Changsha Center for Disease Control and Prevention, Changsha 410008, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Laboratory of Microbiology, Changsha Center for Disease Control and Prevention, Changsha 410008, China.
authors.forename: Linzhi
authors.initials: L
authors.lastname: Li
authors.affiliation: Laboratory of Microbiology, Changsha Center for Disease Control and Prevention, Changsha 410008, China. Electronic address: sunbiancheng2013@163.com.
authors.forename: Biancheng
authors.initials: B
authors.lastname: Sun
mesh_terms: D000956:Antigens, Viral / Q000235:genetics / Q000302:isolation & purification; D004770:Enterovirus / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D004769:Enterovirus Infections / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D005838:Genotype; D006801:Humans; D025202:Molecular Diagnostic Techniques
chemical_list: D000956:Antigens, Viral
keywords: Coxsackievirus A10; Enterovirus; Hand-foot-mouth disease; Real time RT-PCR