title: Mating System and Genetic Structure Across All Known Populations of Dyckia brevifolia: A Clonal, Endemic, and Endangered Rheophyte Bromeliad.
abstract: Dyckia brevifolia is an endemic rheophyte bromeliad that occurs exclusively in patches on rocky banks of the Itajaí-Açu River in southern Brazil. The genetic diversity of all known populations was carried out using allozyme markers and the total numbers of rosettes, reproductive rosettes, and clumps per population were characterized. The mating system was also investigated. Most rosettes were aggregated in groups, and the populations differed significantly in number of rosettes and reproductive rosettes per population. The outcrossing rate obtained was 8.2%, with predominant selfing. The populations presented an average of 1.4 alleles per locus and 27% of polymorphisms. The mean expected genetic diversity was 0.067. Downstream populations showed the highest genetic diversity which could be attributed to hydrochory (unidirectional river flow). Most genetic diversity is distributed among populations (F^ST = 0.402). Natural habitats of D. brevifolia are not recommended for the construction of hydroelectric plants given that it would seriously complicate in situ conservation of this species. Based on the results of this study, it can be concluded that between 35 and 161 reproductive rosettes must be collected for effective ex situ conservation, depending on the targeted population, or seeds collected from 157 seed-rosettes per population.
authors.affiliation: From the Núcleo de Ciências Biológicas e Ambientais, Instituto Federal do Rio Grande do Sul, Rodovia RS 135, Km 25, Distrito Engenheiro Luiz Englert, Caixa Postal 21, Sertão, 99170-000, RS, Brazil (J. M. Rogalski); Herbário Barbosa Rodrigues, Centro, Itajaí, Brazil (Reis); Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa, Brazil (M. Rogalski); and Núcleo de Pesquisas em Florestas Tropicais, Universidade Federal de Santa Catarina, Florianópolis, Brazil (Montagna and dos Reis).
authors.forename: Juliana Marcia
authors.initials: JM
authors.lastname: Rogalski
authors.affiliation: From the Núcleo de Ciências Biológicas e Ambientais, Instituto Federal do Rio Grande do Sul, Rodovia RS 135, Km 25, Distrito Engenheiro Luiz Englert, Caixa Postal 21, Sertão, 99170-000, RS, Brazil (J. M. Rogalski); Herbário Barbosa Rodrigues, Centro, Itajaí, Brazil (Reis); Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa, Brazil (M. Rogalski); and Núcleo de Pesquisas em Florestas Tropicais, Universidade Federal de Santa Catarina, Florianópolis, Brazil (Montagna and dos Reis).
authors.forename: Ademir
authors.initials: A
authors.lastname: Reis
authors.affiliation: From the Núcleo de Ciências Biológicas e Ambientais, Instituto Federal do Rio Grande do Sul, Rodovia RS 135, Km 25, Distrito Engenheiro Luiz Englert, Caixa Postal 21, Sertão, 99170-000, RS, Brazil (J. M. Rogalski); Herbário Barbosa Rodrigues, Centro, Itajaí, Brazil (Reis); Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa, Brazil (M. Rogalski); and Núcleo de Pesquisas em Florestas Tropicais, Universidade Federal de Santa Catarina, Florianópolis, Brazil (Montagna and dos Reis).
authors.forename: Marcelo
authors.initials: M
authors.lastname: Rogalski
authors.affiliation: From the Núcleo de Ciências Biológicas e Ambientais, Instituto Federal do Rio Grande do Sul, Rodovia RS 135, Km 25, Distrito Engenheiro Luiz Englert, Caixa Postal 21, Sertão, 99170-000, RS, Brazil (J. M. Rogalski); Herbário Barbosa Rodrigues, Centro, Itajaí, Brazil (Reis); Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa, Brazil (M. Rogalski); and Núcleo de Pesquisas em Florestas Tropicais, Universidade Federal de Santa Catarina, Florianópolis, Brazil (Montagna and dos Reis).
authors.forename: Tiago
authors.initials: T
authors.lastname: Montagna
authors.affiliation: From the Núcleo de Ciências Biológicas e Ambientais, Instituto Federal do Rio Grande do Sul, Rodovia RS 135, Km 25, Distrito Engenheiro Luiz Englert, Caixa Postal 21, Sertão, 99170-000, RS, Brazil (J. M. Rogalski); Herbário Barbosa Rodrigues, Centro, Itajaí, Brazil (Reis); Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa, Brazil (M. Rogalski); and Núcleo de Pesquisas em Florestas Tropicais, Universidade Federal de Santa Catarina, Florianópolis, Brazil (Montagna and dos Reis).
authors.forename: Maurício Sedrez
authors.initials: MS
authors.lastname: Dos Reis
mesh_terms: D031229:Bromeliaceae / Q000235:genetics; D056727:Endangered Species; D014644:Genetic Variation; D012098:Reproduction
keywords: allozyme; conservation; founder event; genetic diversity; metapopulation; selfing