title: Designer nanoparticle: nanobiotechnology tool for cell biology.
abstract: This article discusses the use of nanotechnology for subcellular compartment isolation and its application towards subcellular omics. This technology review significantly contributes to our understanding on use of nanotechnology for subcellular systems biology. Here we elaborate nanobiotechnology approach of using superparamagnetic nanoparticles (SPMNPs) optimized with different surface coatings for subcellular organelle isolation. Using pulse-chase approach, we review that SPMNPs interacted differently with the cell depending on its surface functionalization. The article focuses on the use of functionalized-SPMNPs as a nanobiotechnology tool to isolate high quality (both purity and yield) plasma membranes and endosomes or lysosomes. Such nanobiotechnology tool can be applied in generating subcellular compartment inventories. As a future perspective, this strategy could be applied in areas such as immunology, cancer and stem cell research.
authors.affiliation: European Molecular Biology Laboratory (EMBL), Grenoble Outstation and Unit of Virus Host-Cell Interactions (UVHCI), UJF-EMBL-CNRS, UMR 5233 Grenoble, France ; Envirotransgene Bio-solutions Global, Chennai, India ; Biotechnology Centre for Oslo, Centre for Molecular Medicine Norway (NCMM), P.O. Box 1137, Blindern, 0318 Oslo, Norway.
authors.forename: Deepak B
authors.identifier: 0000-0002-9328-449X
authors.initials: DB
authors.lastname: Thimiri Govinda Raj
authors.affiliation: Laboratory of Lipid Metabolism and Cancer, O&N I, Herestraat 49, Box 902, 3000 Louvain, Belgium.
authors.forename: Niamat Ali
authors.initials: NA
authors.lastname: Khan
keywords: Nanobiotechnology; Nanoparticles; Subcellular proteomics; Systems biology