title: Isolation and identification of chitin from heavy mineralized skeleton of Suberea clavata (Verongida: Demospongiae: Porifera) marine demosponge.
abstract: Since the discovery of chitin in skeletal structures of sponges (Porifera) in 2007, studies on search of novel species which possess this structural aminopolysaccharide continue up today. The most potential source of chitin is suggested to be localized in the four families of sponges related to the order Verongida (Demospongiae) which nevertheless require further clarification. Here, we report for the first time the isolation and identification of α-chitin from the Suberea clavata demosponge (Aplysinidae: Verongida). Raman spectroscopy, Calcofluor White staining, chitinase test and ESI-MS techniques were used to identify chitin. We suggest that the presence of chitin within fibrous skeletons of diverse species of Verongida order, and, especially in all species of the Aplysinidae family, may be useful for the identification of novel, previously unidentified marine demosponges.
authors.affiliation: Institute of Experimental Physics, TU Bergakademie Freiberg, Leipziger Str. 23, 09599 Freiberg, Germany.
authors.forename: Hermann
authors.initials: H
authors.lastname: Ehrlich
authors.affiliation: Institute of Experimental Physics, TU Bergakademie Freiberg, Leipziger Str. 23, 09599 Freiberg, Germany; Current address: European XFEL, Albert-Einstein-Ring 19, 22761 Hamburg, Germany.
authors.forename: Vasilii V
authors.initials: VV
authors.lastname: Bazhenov
authors.affiliation: UMR 241 EIO, IRD - BP529 - 98713 Papeete, Polynésie Française, France.
authors.forename: Cecile
authors.initials: C
authors.lastname: Debitus
authors.affiliation: Naturalis Biodiversity Centre, P.O. Box 9517, Leiden 2300 RA, the Netherlands.
authors.forename: Nicole
authors.initials: N
authors.lastname: de Voogd
authors.affiliation: Clinical Sensoring and Monitoring, Department of Anesthesiology and Intensive Care Medicine, Faculty of Medicine, TU Dresden, Fetscher Str. 74, D-01307 Dresden, Germany.
authors.forename: Roberta
authors.initials: R
authors.lastname: Galli
authors.affiliation: Leibniz Institute of Polymer Research Dresden, Hohestraße 6, 01069 Dresden, Germany.
authors.forename: Mikhail V
authors.initials: MV
authors.lastname: Tsurkan
authors.affiliation: Institute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, 61131 Poznan, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Wysokowski
authors.affiliation: Clinical Sensoring and Monitoring, Department of Anesthesiology and Intensive Care Medicine, Faculty of Medicine, TU Dresden, Fetscher Str. 74, D-01307 Dresden, Germany.
authors.forename: Heike
authors.initials: H
authors.lastname: Meissner
authors.affiliation: Aksaray University, Faculty of Science and Letters, Department of Biotechnology and Molecular Biology, 68100, Aksaray, Turkey.
authors.forename: Esra
authors.initials: E
authors.lastname: Bulut
authors.affiliation: Aksaray University, Faculty of Science and Letters, Department of Biotechnology and Molecular Biology, 68100, Aksaray, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Kaya
authors.affiliation: Institute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, 61131 Poznan, Poland.
authors.forename: Teofil
authors.initials: T
authors.lastname: Jesionowski
mesh_terms: D000818:Animals; D002686:Chitin / Q000032:analysis* / Q000302:isolation & purification* / Q000378:metabolism; D002688:Chitinases / Q000378:metabolism; D008903:Minerals / Q000378:metabolism*; D011161:Porifera / Q000737:chemistry* / Q000378:metabolism; D012863:Skeleton / Q000378:metabolism*
chemical_list: D008903:Minerals; D002686:Chitin; D002688:Chitinases
keywords: Chitin; Suberea clavata; sponges