title: Nucleic acid detection using BRET-beacons based on bioluminescent protein-DNA hybrids.
abstract: Bioluminescent molecular beacons have been developed using a modular design approach that relies on BRET between the bright luciferase NanoLuc and a Cy3 acceptor. While classical molecular beacons are hampered by background fluorescence and scattering, these BRET-beacons allow detection of low pM concentrations of nucleic acids directly in complex media.
authors.affiliation: Laboratory of Chemical Biology and Institute for Complex Molecular Systems Eindhoven, University of Technology, Den Dolech 2, 5600 MB, Eindhoven, The Netherlands. m.merkx@tue.nl.
authors.forename: Wouter
authors.initials: W
authors.lastname: Engelen
authors.affiliation: Laboratory of Chemical Biology and Institute for Complex Molecular Systems Eindhoven, University of Technology, Den Dolech 2, 5600 MB, Eindhoven, The Netherlands. m.merkx@tue.nl.
authors.forename: Kayleigh M
authors.initials: KM
authors.lastname: van de Wiel
authors.affiliation: Laboratory of Chemical Biology and Institute for Complex Molecular Systems Eindhoven, University of Technology, Den Dolech 2, 5600 MB, Eindhoven, The Netherlands. m.merkx@tue.nl.
authors.forename: Lenny H H
authors.initials: LHH
authors.lastname: Meijer
authors.affiliation: Laboratory of Chemical Biology and Institute for Complex Molecular Systems Eindhoven, University of Technology, Den Dolech 2, 5600 MB, Eindhoven, The Netherlands. m.merkx@tue.nl.
authors.forename: Bedabrata
authors.initials: B
authors.lastname: Saha
authors.affiliation: Laboratory of Chemical Biology and Institute for Complex Molecular Systems Eindhoven, University of Technology, Den Dolech 2, 5600 MB, Eindhoven, The Netherlands. m.merkx@tue.nl.
authors.forename: Maarten
authors.identifier: http://orcid.org/0000-0001-9484-3882
authors.initials: M
authors.lastname: Merkx
mesh_terms: D060807:Bioluminescence Resonance Energy Transfer Techniques; D004247:DNA / Q000032:analysis*; D005453:Fluorescence; D008164:Luminescent Proteins / Q000737:chemistry*
chemical_list: D008164:Luminescent Proteins; D004247:DNA