title: Effect of Cis-palmitoleic acid supplementation on inflammation and expression of HNF4γ, HNF4α and IL6 in patients with ulcerative colitis.
abstract: BACKGROUND
  Cis-palmitoleic acid (Omega-7 fatty acid) is a monounsaturated fatty acid (MUFA) associated with anti-inflammatory process through specific protein interactions such as HNF4γ and HNF4α, these two genes are related to the immune response in ulcerative colitis (UC) they may act as a mediator of anti-inflammatory action. The aim of this study was to evaluate the effect of Cis-palmitoleic acid supplementation on inflammatory activity and the expression of genes HNF4γ, HNF4α and IL6 in the colonic mucosa of patients with active UC.
  
  
  METHODS
  A double-blind, randomized, placebo-controlled pilot study was conducted in 20 patients with UC. A dose of 720 mg/day of Cis-palmitoleic acid was orally administered during 8 weeks and Mayo Clinic score was used for the assessment of clinical activity in UC before and after treatment with Cis-palmitoleic acid and placebo.
  
  
  RESULTS
  A total of 20 patients with UC were randomized to receive Cis-palmitoleic acid or placebo. Significant changes in the biochemical markers of inflammation were found in UC patients before and after treatment with Cis-palmitoleic acid vs. placebo such as total protein (P=0.02), hs-CRP (P=0.04) and ESR (P<0.05). The gene expression of HNF4γ and HNF4α were found to be increased in the Cis-palmitoleic acid group compared to placebo group (P=0.05 and P=0.07 respectively) as well as significant reduction upon IL6 expression in the Cis-palmitoleic acid group (P=0.005).
  
  
  CONCLUSIONS
  Cis-palmitoleic acid as co-adjuvant therapy for 8 weeks seems to decrease the inflammatory activity through the increased expression of HNF4α and HNF4γ in patients with UC.
authors.affiliation: Department of Gastroenterology, Inflammatory Bowel Disease Clinic, National Institute of Medical Sciences and Nutrition &quot;Salvador Zubirán&quot; (INCMNSZ), Mexico City, Mexico.
authors.forename: Nallely
authors.initials: N
authors.lastname: Bueno-Hernández
authors.affiliation: Gastroenterology Laboratory, National Institute of Medical Sciences and Nutrition &quot;Salvador Zubirán&quot; (INCMNSZ), Mexico City, Mexico.
authors.forename: María S
authors.initials: MS
authors.lastname: Sixtos-Alonso
authors.affiliation: Nutrition Unit, National Institute of Medical Sciences and Nutrition &quot;Salvador Zubirán&quot; (INCMNSZ), Mexico City, Mexico.
authors.forename: María Del Pilar
authors.initials: MDP
authors.lastname: Milke García
authors.affiliation: Department of Gastroenterology, Inflammatory Bowel Disease Clinic, National Institute of Medical Sciences and Nutrition &quot;Salvador Zubirán&quot; (INCMNSZ), Mexico City, Mexico - kazuofurusho@hotmail.com.
authors.forename: Jesús K
authors.initials: JK
authors.lastname: Yamamoto-Furusho
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D003093:Colitis, Ulcerative / Q000188:drug therapy*; D004311:Double-Blind Method; D005229:Fatty Acids, Monounsaturated / Q000627:therapeutic use*; D005260:Female; D051557:Hepatocyte Nuclear Factor 4 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000188:drug therapy; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D010865:Pilot Projects; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D015415:Biomarkers; D005229:Fatty Acids, Monounsaturated; D051557:Hepatocyte Nuclear Factor 4; C508600:IL6 protein, human; D015850:Interleukin-6; D012333:RNA, Messenger; C008757:palmitoleic acid