title: Circulating endothelial microparticles andmiR-92ain acute myocardial infarction.
abstract: Microparticles (MPs) and miRNAs have been shown to play important roles in coronary artery disease (CAD) by monitoring endothelial dysfunction. The present study aims to investigate the diagnostic value of endothelial MPs (EMPs) and miRNAs (miR-92aormiR-23a) as biomarkers in distinguishing patients with acute myocardial infarction (AMI) from those with CAD. Plasma samples from 37 patients with AMI, 42 patients with stable CAD (SCAD), and 35 healthy adults were collected for investigation in the present study. The numbers of CD31+/CD42b- MPs, CD31+/CD42b+ MPs, and CD31-/CD42b- MPs were measured by flow cytometry and the levels ofmiR-92aandmiR-23awere analyzed using reverse transcription-quantitative PCR. Moreover, cardiac troponin I (cTnI) expression was detected by ELISA to serve as a routine diagnostic parameter. The number of CD31+/CD42b- was higher in AMI group than those in SCAD and healthy groups. Besides, the expression ofmiR-92awas higher in AMI group compared with two other groups. Furthermore, evidence showed that there was a positive correlation between the levels of CD31+/CD42b- MPs andmiR-92aFinally, the receiver operating characteristic (ROC) curve revealed that the area value under the curve of CD31+/CD42b- MPs,miR-92aand cTnI was 0.893, 0.888, and 0.912 respectively. CD31+/CD42b- MPs andmiR-92amight have great potential to provide diagnostic value for AMI and could probably regulate the endothelial dysfunction in AMI patients.
authors.affiliation: Department of Cardiology, Beijing An Zhen Hospital of the Capital University of Medical Sciences, Beijing 100029, China, Anzhen Road, Chaoyang District.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China, Tian Tan Xili, Beijing.
authors.forename: Junjun
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Department of Cardiology, Beijing An Zhen Hospital of the Capital University of Medical Sciences, Beijing 100029, China, Anzhen Road, Chaoyang District.
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
authors.affiliation: Department of Cardiology, Beijing An Zhen Hospital of the Capital University of Medical Sciences, Beijing 100029, China, Anzhen Road, Chaoyang District.
authors.forename: Changyan
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Cardiology, Beijing An Zhen Hospital of the Capital University of Medical Sciences, Beijing 100029, China, Anzhen Road, Chaoyang District.
authors.forename: Junmeng
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, Beijing An Zhen Hospital of the Capital University of Medical Sciences, Beijing 100029, China, Anzhen Road, Chaoyang District.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Ren
authors.affiliation: Department of Cardiology, Beijing An Zhen Hospital of the Capital University of Medical Sciences, Beijing 100029, China, Anzhen Road, Chaoyang District.
authors.forename: Yu
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Department of Cardiology, Beijing An Zhen Hospital of the Capital University of Medical Sciences, Beijing 100029, China, Anzhen Road, Chaoyang District.
authors.forename: Bin
authors.initials: B
authors.lastname: Nie
authors.affiliation: Department of Cardiology, Beijing An Zhen Hospital of the Capital University of Medical Sciences, Beijing 100029, China, Anzhen Road, Chaoyang District.
authors.forename: Quan
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Cardiology, Beijing An Zhen Hospital of the Capital University of Medical Sciences, Beijing 100029, China, Anzhen Road, Chaoyang District lybjazh@163.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
mesh_terms: D000704:Analysis of Variance; D015415:Biomarkers / Q000097:blood; D055252:Cell-Derived Microparticles; D003324:Coronary Artery Disease / Q000097:blood / Q000175:diagnosis; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood / Q000175:diagnosis*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000097:blood; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000032:analysis; D011379:Prognosis; D019210:Troponin I / Q000097:blood
chemical_list: D015415:Biomarkers; C552430:MIRN23a microRNA, human; C541874:MIRN92 microRNA, human; D035683:MicroRNAs; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D019038:Platelet Glycoprotein GPIb-IX Complex; D019210:Troponin I
keywords: acute myocardial infarction; microRNAs; microparticles