title: Complete genome analysis of Serratia marcescens RSC-14: A plant growth-promoting bacterium that alleviates cadmium stress in host plants.
abstract: Serratia marcescens RSC-14 is a Gram-negative bacterium that was previously isolated from the surface-sterilized roots of the Cd-hyperaccumulator Solanum nigrum. The strain stimulates plant growth and alleviates Cd stress in host plants. To investigate the genetic basis for these traits, the complete genome of RSC-14 was obtained by single-molecule real-time sequencing. The genome of S. marcescens RSC-14 comprised a 5.12-Mbp-long circular chromosome containing 4,593 predicted protein-coding genes, 22 rRNA genes, 88 tRNA genes, and 41 pseudogenes. It contained genes with potential functions in plant growth promotion, including genes involved in indole-3-acetic acid (IAA) biosynthesis, acetoin synthesis, and phosphate solubilization. Moreover, annotation using NCBI and Rapid Annotation using Subsystem Technology identified several genes that encode antioxidant enzymes as well as genes involved in antioxidant production, supporting the observed resistance towards heavy metals, such as Cd. The presence of IAA pathway-related genes and oxidative stress-responsive enzyme genes may explain the plant growth-promoting potential and Cd tolerance, respectively. This is the first report of a complete genome sequence of Cd-tolerant S. marcescens and its plant growth promotion pathway. The whole-genome analysis of this strain clarified the genetic basis underlying its phenotypic and biochemical characteristics, underpinning the beneficial interactions between RSC-14 and plants.
authors.affiliation: School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu, Republic of Korea.|Department of Plant and Microbial Biology, University of California, Berkeley, CA, United States of America.
authors.forename: Abdur Rahim
authors.initials: AR
authors.lastname: Khan
authors.affiliation: School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Gun-Seok
authors.initials: GS
authors.lastname: Park
authors.affiliation: School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Sajjad
authors.initials: S
authors.lastname: Asaf
authors.affiliation: School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Sung-Jun
authors.initials: SJ
authors.lastname: Hong
authors.affiliation: School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Byung Kwon
authors.initials: BK
authors.lastname: Jung
authors.affiliation: School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Jae-Ho
authors.identifier: http://orcid.org/0000-0001-6450-9787
authors.initials: JH
authors.lastname: Shin
mesh_terms: D000093:Acetoin / Q000378:metabolism; D002104:Cadmium / Q000633:toxicity*; D005798:Genes, Bacterial; D054884:Host-Pathogen Interactions / Q000235:genetics*; D007210:Indoleacetic Acids / Q000378:metabolism; D058977:Molecular Sequence Annotation; D018384:Oxidative Stress; D010710:Phosphates / Q000378:metabolism; D012706:Serratia marcescens / Q000235:genetics*; D032322:Solanum / Q000187:drug effects / Q000382:microbiology
chemical_list: D007210:Indoleacetic Acids; D010710:Phosphates; D002104:Cadmium; C030737:indoleacetic acid; D000093:Acetoin