title: Single Nucleotide rs760370 Polymorphism at the Main Ribavirin Transporter Gene Detection by PCR-RFLP Assay Compared with the TaqMan Assay and Its Relation to Sustained Virological Response in Chronic HCV Patients Treated with Pegylated Interferon-Ribavirin Therapy.
abstract: Ribavirin clearly plays a role in chronic hepatitis C treatment response. The equilibrative nucleoside transporter-1 codified by SLC29A1 gene has been associated with ribavirin uptake into hepatocytes and erythrocytes. rs760370A>G single nucleotide polymorphism (SNP) at the SLC29A1 gene may have a role in ribavirin-based regimen treatment response. Accuracy of the polymerase-chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay compared with the TaqMan assay for the detection of the SNP rs760370 at the main ribavirin transporter gene and its relation to sustained virological response in chronic hepatitis C virus (HCV) patients treated with pegylated interferon-ribavirin therapy. The study included 100 chronic HCV patients who were treated with pegylated interferon-ribavirin therapy. The patients were categorized according to the treatment response into responders (50 patients) and null responders (50 patients). rs760370 SNP was measured using TaqMan 5-nuclease assay and by the newly developed PCR-based RFLP assay. The overall accuracy of the newly developed PCR-RFLP assay compared with the TaqMan assay for rs760370 polymorphism detection was 100%. Allelic frequencies at rs760370 were as follows: A/A genotype (28%), A/G genotype (58%), and G/G genotype (14%). Treatment response was not significantly related with rs760370 polymorphism (P = 0.5). Ribavirin-induced anemia was good predictor of sustained virological response (P = 0.001), but was not related to rs760370 polymorphism (P = 0.92). PCR-RFLP assay is an accurate, cost-effective method in the detection of rs760370 compared with TaqMan assay. rs760370 SNP cannot serve as predictor of response in chronic HCV patients treated with interferon ribavirin therapy.
authors.affiliation: 1 Endemic Medicine Department and Hepatology Unit, Faculty of Medicine, Cairo University , Cairo, Egypt .
authors.forename: Rabab
authors.initials: R
authors.lastname: Fouad
authors.affiliation: 1 Endemic Medicine Department and Hepatology Unit, Faculty of Medicine, Cairo University , Cairo, Egypt .
authors.forename: Khaled
authors.initials: K
authors.lastname: Zachariah
authors.affiliation: 1 Endemic Medicine Department and Hepatology Unit, Faculty of Medicine, Cairo University , Cairo, Egypt .
authors.forename: Marwa
authors.initials: M
authors.lastname: Khairy
authors.affiliation: 2 Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University , Cairo, Egypt .
authors.forename: Mervat
authors.initials: M
authors.lastname: Khorshied
authors.affiliation: 3 Topical Medicine Department, National Research Center, Cairo, Egypt .
authors.forename: Wafaa
authors.initials: W
authors.lastname: Ezzat
authors.affiliation: 2 Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University , Cairo, Egypt .
authors.forename: Marwa M
authors.initials: MM
authors.lastname: Sheta
authors.affiliation: 3 Topical Medicine Department, National Research Center, Cairo, Egypt .
authors.forename: Ahmed
authors.initials: A
authors.lastname: Heiba
mesh_terms: D000328:Adult; D000483:Alleles; D004359:Drug Therapy, Combination; D033721:Equilibrative Nucleoside Transporter 1 / Q000235:genetics*; D005260:Female; D005838:Genotype; D016174:Hepacivirus / Q000187:drug effects; D019698:Hepatitis C, Chronic / Q000235:genetics* / Q000473:pathology / Q000821:virology*; D006801:Humans; D016898:Interferon-alpha / Q000627:therapeutic use; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction; D012254:Ribavirin / Q000627:therapeutic use; D016896:Treatment Outcome
chemical_list: D033721:Equilibrative Nucleoside Transporter 1; D016898:Interferon-alpha; C438649:SLC29A1 protein, human; D012254:Ribavirin
keywords: PCR-RFLP assay; TaqMan assay; chronic HCV; ribavirin; rs760370-SVR