title: Development of ciprofloxacin-loaded contact lenses using fluorous chemistry.
abstract: In this work, we developed a simple method to load drugs into commercially available contact lenses utilizing fluorous chemistry. We demonstrated this method using model compounds including fluorous-tagged fluorescein and antibiotic ciprofloxacin. We showed that fluorous interactions facilitated the loading of model molecules into fluorocarbon-containing contact lenses, and that the release profiles exhibited sustained release. Contact lenses loaded with fluorous-tagged ciprofloxacin exhibited antimicrobial activity against Pseudomonas aeruginosa in vitro, while no cytotoxicity towards human corneal epithelial cells was observed. To mimic the tear turnover, we designed a porcine eye infection model under flow conditions. Significantly, the modified lenses also exhibited antimicrobial efficacy against Pseudomonas aeruginosa in the ex vivo infection model. Overall, utilizing fluorous chemistry, we can construct a drug delivery system that exhibits high drug loading capacity, sustained drug release, and robust biological activity.
authors.affiliation: College of Optometry, University of Houston, Houston, TX, 77204, USA. Electronic address: gqin@central.uh.edu.
authors.forename: Guoting
authors.initials: G
authors.lastname: Qin
authors.affiliation: Department of Chemistry, University of Houston, Houston, TX, 77204, USA.
authors.forename: Zhiling
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Department of Chemistry, University of Houston, Houston, TX, 77204, USA.
authors.forename: Siheng
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Optometry, University of Houston, Houston, TX, 77204, USA.
authors.forename: Alison M
authors.initials: AM
authors.lastname: McDermott
authors.affiliation: Department of Chemistry, University of Houston, Houston, TX, 77204, USA. Electronic address: cai@uh.edu.
authors.forename: Chengzhi
authors.initials: C
authors.lastname: Cai
mesh_terms: D060433:Administration, Ophthalmic; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D002939:Ciprofloxacin / Q000008:administration & dosage* / Q000737:chemistry*; D003261:Contact Lenses; D004343:Drug Implants / Q000008:administration & dosage* / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D015818:Eye Infections, Bacterial / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D066298:In Vitro Techniques; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D013552:Swine; D016896:Treatment Outcome
chemical_list: D000900:Anti-Bacterial Agents; D004343:Drug Implants; D002939:Ciprofloxacin
keywords: Antimicrobial activity; Contact lenses; Drug delivery; Fluorous chemistry