title: A new phosphothreonine lyase electrochemical immunosensor for detecting Salmonella based on horseradish peroxidase/GNPs-thionine/chitosan.
abstract: In the current study, a novel double-layer gold nanoparticles- electrochemical immunosensor electrode (DGN-EIE) immobilized with Salmonella plasmid virulence C (SpvC) antibody was developed. To increase the fixed quantity of antibodies and electrochemical signal, an electrochemical biosensing signal amplification system was utilized with gold nanoparticles-thionine-chitosan absorbing horseradish peroxidase (HRP). In addition, the SpvC monoclonal antibodies (derived from Balb/c mice) were prepared and screened with a high affinity to SpvC. To evaluate the quality of DGN-EIE, the amperometric I-t curve method was applied to determine Salmonella in PBS. The results showed that the response current had a good linear correlation with the bacterial quantity ranged from 1.0 × 101-5.0 × 104cfu/mL. The lowest detection limit was found at 5 cfu/mL. Furthermore, the proposed immunosensor has been demonstrated with high sensitivity, good selectivity and reproducibility. Apparently, DGN-EIE may be a very useful tool for monitoring the bacteria.
authors.affiliation: Biotechnology & Food Science College, Tianjin University of Commerce, Tianjin, 300314, China.
authors.forename: Dingqiang
authors.initials: D
authors.lastname: Lu
authors.affiliation: Biotechnology & Food Science College, Tianjin University of Commerce, Tianjin, 300314, China. pgc@tjcu.edu.cn.|Tianjin Key Laboratory of Food Biotechnology, Tianjin, 300314, China. pgc@tjcu.edu.cn.
authors.forename: Guangchang
authors.initials: G
authors.lastname: Pang
authors.affiliation: Biotechnology & Food Science College, Tianjin University of Commerce, Tianjin, 300314, China. xjbo@tjcu.edu.cn.|Tianjin Key Laboratory of Food Biotechnology, Tianjin, 300314, China. xjbo@tjcu.edu.cn.
authors.forename: Junbo
authors.initials: J
authors.lastname: Xie
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D019757:Carbon-Oxygen Lyases / Q000276:immunology*; D048271:Chitosan / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism*; D007118:Immunoassay; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D010640:Phenothiazines / Q000737:chemistry*; D012475:Salmonella / Q000201:enzymology / Q000302:isolation & purification*; D013997:Time Factors
chemical_list: D055910:Antibodies, Immobilized; D010640:Phenothiazines; D006046:Gold; D048271:Chitosan; D006735:Horseradish Peroxidase; D019757:Carbon-Oxygen Lyases; C009469:thionine
keywords: Electrochemical immunosensor; Phosphothreonine lyase; Salmonella; Salmonella plasmid virulence C