title: Rapid Analysis of Components inCoptis chinensisFranch by Ultra-Performance Liquid Chromatography with Quadrupole Time-of-Flight Mass Spectrometry.
abstract: BACKGROUND
  Coptis chinensisFranch is a traditional Chinese medical herb.
  
  
  OBJECTIVE
  In this article, ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used to rapidly, qualitatively, and comprehensively identify the components inCoptis chinensisFranch.
  
  
  METHODS
  Chromatographic separation was achieved on an Agilent Zorbax RRHD Eclipse Plus C18column. The mobile phase consisted of 0.1% formic acid water (A) and 0.1% formic acid acetonitrile (B) with a gradient program. Qualitative analysis was performed on an Agilent 6540 quadrupole time-of-flight mass spectrometer, which was equipped with a Dual AJS ESI source operating in negative mode.
  
  
  RESULTS
  A total of 30 alkaloid and non-alkaloid components ofCoptis chinensisFranch were identified in only 14 min.
  
  
  CONCLUSIONS
  This study helped to provide a basis for the quality control ofCoptis chinensisFranch.
  
  
  CONCLUSIONS
  Qualitative analysis method of chlorogenic alkaloids and non-alkaloids inCoptis chinensisFranch is developed by Ultra-performance liquid chromatography with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) method.Established UPLC-Q-TOF-MS/MS analysis method is validated with rapidness and accuracy.The developed method was successfully applied for qualitative analysis ofCoptis chinensisFranch sample collected from cultivation place in China.Abbreviations used:Q-TOF-MS: quadrupole time-of-flight mass spectrometry, UPLC: ultra-performance liquid chromatography, pos: positive, neg: negative. Q-TOF-MS: quadrupole time-of-flight mass spectrometry, UPLC: ultra-performance liquid chromatography, pos: positive, neg: negative. UPLC: ultra-performance liquid chromatography, pos: positive, neg: negative. pos: positive, neg: negative. neg: negative.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Dongzhimen, Beijing; Laboratory of Cell Pharmacology, College of Pharmaceutical Sciences, Hebei University, Baoding, China.
authors.forename: Peng-Peng
authors.initials: PP
authors.lastname: Tian
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Dongzhimen, Beijing; Laboratory of Cell Pharmacology, College of Pharmaceutical Sciences, Hebei University, Baoding, China.
authors.forename: Xiao-Xu
authors.initials: XX
authors.lastname: Zhang
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Dongzhimen, Beijing.
authors.forename: Hong-Ping
authors.initials: HP
authors.lastname: Wang
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Dongzhimen, Beijing; Henan University of Traditional Chinese Medicine, Zhengzhou, China.
authors.forename: Pu-Ling
authors.initials: PL
authors.lastname: Li
authors.affiliation: Laboratory of Cell Pharmacology, College of Pharmaceutical Sciences, Hebei University, Baoding, China; Drug Quality Control Key Laboratory of Hebei Province, Hebei University, Baoding, China.
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Liu
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Dongzhimen, Beijing.
authors.forename: Shao-Jing
authors.initials: SJ
authors.lastname: Li
keywords: Alkaloids; Coptis chinensis Franch; Q-TOF; UPLC; non-alkaloids