title: Impact of Delayed Infusion Time in Umbilical Cord Blood Transplantation.
abstract: In umbilical cord blood (UCB) transplantation, UCB units are typically thawed, washed, and infused into the patient as rapidly as possible. In some instances there is a delay in the time from the unit thaw and wash procedure to infusion into the patient. Therefore, we examined the effect of thaw duration time on engraftment outcomes in 567 patients undergoing UCB transplantation. With a range of 32 to 523 minutes, a prolonged thaw duration had no obvious effect on the incidence of neutrophil engraftment or time to recovery. This was true for recipients of single UCB transplantation (incidence: 97% versus 93%, P = .13; time to neutrophil recovery: 21 days versus 21 days, P = .32; and platelet recovery: 79% versus 78%, P = .48), and similar results were observed in double UCB transplantation (time to neutrophil engraftment: 20 days versus 19 days, P = .71). However, there was a trend toward better platelet recovery in recipients of double UCB transplants with prolonged thaw duration (HR, 1.28; P = .06). In conclusion, this study demonstrates prolonged thaw duration has no detrimental effect on engraftment after single or double UCB transplantation.
authors.affiliation: Kids Cancer Centre, Sydney Children's Hospital, Randwick, New South Wales, Australia.
authors.forename: Richard
authors.initials: R
authors.lastname: Mitchell
authors.affiliation: Pediatric Blood and Marrow Transplantation Program, University of Minnesota, Minneapolis, Minnesota.
authors.forename: John E
authors.initials: JE
authors.lastname: Wagner
authors.affiliation: Division of Hematology, Oncology and Transplantation, University of Minnesota, Minneapolis, Minnesota.
authors.forename: Claudio
authors.initials: C
authors.lastname: Brunstein
authors.affiliation: Biostatistic Core, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota.
authors.forename: Qing
authors.initials: Q
authors.lastname: Cao
authors.affiliation: Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota.
authors.forename: David H
authors.initials: DH
authors.lastname: McKenna
authors.affiliation: Pediatric Blood and Marrow Transplantation Program, University of Minnesota, Minneapolis, Minnesota. Electronic address: verneris@umn.edu.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Verneris
mesh_terms: D001792:Blood Platelets / Q000166:cytology; D036101:Cord Blood Stem Cell Transplantation / Q000379:methods* / Q000592:standards; D015925:Cryopreservation; D005312:Fetal Blood / Q000166:cytology; D006085:Graft Survival; D006801:Humans; D009504:Neutrophils / Q000166:cytology; D020127:Recovery of Function; D013997:Time Factors
keywords: Cord blood; Engraftment; Thaw time