title: Direct Production of Microstructured Surfaces for Planar Chromatography Using 3D Printing.
abstract: Through optimization of the printing process and orientation, a suitably developed surface area has been realized upon a 3D printed polymer substrate to facilitate chromatographic separations in a planar configuration. Using an Objet Eden 260VS 3D printer, polymer thin layer chromatography platforms were directly fabricated without any additional surface functionalization and successfully applied to the separation of various dye and protein mixtures. The print material was characterized using gas chromatography coupled to mass spectrometry and spectroscopic techniques such as infrared and Raman. Preliminary studies included the separation of colored dyes, whereby the separation performance could be visualized optically. Subsequent separations were achieved using fluorescent dyes and fluorescently tagged proteins. The separation of proteins was affected by differences in the isoelectric point (pI) and the ion exchange properties of the printed substrate. The simple chromatographic separations are the first achieved using an unmodified 3D printed stationary phase.
authors.affiliation: ARC Centre of Excellence for Electromaterials Science, University of Tasmania , Sandy Bay, Hobart 7001, Tasmania, Australia.|Australian Centre for Research on Separation Science, School of Physical Sciences, University of Tasmania , Sandy Bay, Hobart 7001, Tasmania, Australia.
authors.forename: Niall P
authors.initials: NP
authors.lastname: Macdonald
authors.affiliation: Australian Centre for Research on Separation Science, School of Physical Sciences, University of Tasmania , Sandy Bay, Hobart 7001, Tasmania, Australia.
authors.forename: Sinead A
authors.initials: SA
authors.lastname: Currivan
authors.affiliation: Australian Centre for Research on Separation Science, School of Physical Sciences, University of Tasmania , Sandy Bay, Hobart 7001, Tasmania, Australia.
authors.forename: Laura
authors.initials: L
authors.lastname: Tedone
authors.affiliation: ARC Centre of Excellence for Electromaterials Science, University of Tasmania , Sandy Bay, Hobart 7001, Tasmania, Australia.|Australian Centre for Research on Separation Science, School of Physical Sciences, University of Tasmania , Sandy Bay, Hobart 7001, Tasmania, Australia.
authors.forename: Brett
authors.identifier: 0000-0001-6373-6582
authors.initials: B
authors.lastname: Paull