title: Surface Plasmon Resonance Investigations of Bioselective Element Based on the Recombinant Protein A for Immunoglobulin Detection.
abstract: The developed surface plasmon resonance (SPR) biosensor based on the recombinant Staphylococcal protein A with an additional cysteine residue (SPA-Cys) used as a biorecognition component showed a good selectivity and sensitivity for the immunoglobulin detection. The developed biosensor with SPA-Cys-based bioselective element can also be used as a first step of immunosensor creation. The successful immobilization of SPA-Cys on the nanolayer gold sensor surface of the SPR spectrometer was performed. The efficiency of blocking nonspecific sorption sites on the sensor surface with milk proteins, gelatin, BSA, and HSA was studied, and a rather high efficiency of using gelatin was confirmed. The SPR biosensor selectively interacted with IgG and did not interact with the control proteins. The linear dependence of the sensor response on the IgG concentration in the range from 2 to 10 μg/ml was shown. Using the calibration curve, the IgG concentration was measured in the model samples. The determined concentrations are in good agreement (r2= 0.97) with the given concentration of IgG.
authors.affiliation: Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, 150 Zabolotnogo Str., 03680, Kyiv, Ukraine. a.bakhmachuk@gmail.com.|Institute of High Technologies, Taras Shevchenko National University of Kyiv, 2, korp.5, Pr. Akademika Hlushkova, Kyiv, 03022, Ukraine. a.bakhmachuk@gmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Bakhmachuk
authors.affiliation: Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, 150 Zabolotnogo Str., 03680, Kyiv, Ukraine.|State Institute of Genetic and Regenerative Medicine, NAMS of Ukraine, 57/3, Velyka Vasyl'kivska Str., Kyiv, 03150, Ukraine.
authors.forename: O
authors.initials: O
authors.lastname: Gorbatiuk
authors.affiliation: Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, 150 Zabolotnogo Str., 03680, Kyiv, Ukraine.
authors.forename: A
authors.initials: A
authors.lastname: Rachkov
authors.affiliation: Institute of Pediatrics, Obstetrics and Gynecology, NAMS of Ukraine, 8, Maiborody Str., Kyiv, 04050, Ukraine.
authors.forename: B
authors.initials: B
authors.lastname: Dons'koi
authors.affiliation: V.E. Lashkaryov Institute of Semiconductor Physics, NAS of Ukraine, 41, Prospect Nauki, Kyiv, 03028, Ukraine.
authors.forename: R
authors.initials: R
authors.lastname: Khristosenko
authors.affiliation: V.E. Lashkaryov Institute of Semiconductor Physics, NAS of Ukraine, 41, Prospect Nauki, Kyiv, 03028, Ukraine.
authors.forename: I
authors.initials: I
authors.lastname: Ushenin
authors.affiliation: Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, 150 Zabolotnogo Str., 03680, Kyiv, Ukraine.|Institute of High Technologies, Taras Shevchenko National University of Kyiv, 2, korp.5, Pr. Akademika Hlushkova, Kyiv, 03022, Ukraine.
authors.forename: V
authors.initials: V
authors.lastname: Peshkova
authors.affiliation: Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, 150 Zabolotnogo Str., 03680, Kyiv, Ukraine.|Institute of High Technologies, Taras Shevchenko National University of Kyiv, 2, korp.5, Pr. Akademika Hlushkova, Kyiv, 03022, Ukraine.
authors.forename: A
authors.initials: A
authors.lastname: Soldatkin
keywords: Affinity biosensor; Immunoglobulin; Protein immobilization; Recombinant Staphylococcal protein A; Surface plasmon resonance