title: [Effect of IL-1β or TNF-α-treated human decidual cells on balance between Th1 and Th2 or Th17 and Treg immunity].
abstract: To investigate the effect of IL-1β or TNF-α-treated human decidual cells on the balance between Th1 and Th2 or Th17 and Treg.  Methods: Human decidual cells were isolated and treated with estrogen (E2) and progesterone (P) for 7 days and then with IL-1β or TNF-α for 24 h. The culture medium was collected to prepare conditioned medium. The T cells isolated from human decidual tissue were cultured in presence of conditioned medium for 72 h. ELISA was used to detect concentration of IFN-γ, IL-2, IL-5, IL-17 IL-10, and TGF-1β in supernatants and quantitative real-time PCR was used to detect the mRNA expression of IFN-γ, IL-17, IL-10, and TGF-1β.  Results: The levels of IFN-γ, IL-2 and IL-17 were significantly increased when T cells were cultured in the conditioned medium compared with the control group (E2+P), while the levels of IL-5, IL-10, and TGF-1β were not significantly changed. The mRNA expressions of IFN-γ and IL-17 mRNA were significantly increased when T cells were cultured in the conditioned medium compared with the control group (E2+P), while the mRNA expression of IL-10 and TGF-1β was not significantly different.  Conclusion: Human decidual cells treated by IL-1β or TNF-α can shift the balance of Th1 and Th2 or Th17 and Treg toward Th1 and Th17 immunity.
authors.affiliation: Department of Obstetrics & Gynecology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Xianqing
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Obstetrics & Gynecology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Fang
authors.affiliation: Department of Obstetrics & Gynecology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Meifang
authors.initials: M
authors.lastname: Nie
authors.affiliation: Department of Obstetrics & Gynecology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ding
authors.affiliation: Department of Obstetrics & Gynecology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Xiaomeng
authors.initials: X
authors.lastname: Xia
mesh_terms: D002454:Cell Differentiation / Q000276:immunology*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D003656:Decidua / Q000187:drug effects* / Q000276:immunology*; D005260:Female; D006801:Humans; D056747:Immunomodulation / Q000502:physiology*; D007371:Interferon-gamma / Q000187:drug effects / Q000276:immunology; D016753:Interleukin-10 / Q000378:metabolism; D020381:Interleukin-17 / Q000276:immunology; D053583:Interleukin-1beta / Q000494:pharmacology; D007376:Interleukin-2 / Q000276:immunology; D015848:Interleukin-5 / Q000378:metabolism; D012333:RNA, Messenger; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects* / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects* / Q000276:immunology*; D018417:Th1 Cells; D058504:Th17 Cells; D053773:Transforming Growth Factor beta1 / Q000187:drug effects / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D017077:Culture Media, Conditioned; C508609:IL10 protein, human; C508717:IL17A protein, human; D020381:Interleukin-17; D053583:Interleukin-1beta; D007376:Interleukin-2; D015848:Interleukin-5; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma