title: Efficacy of epiphytic bacteria to prevent northern leaf blight caused by Exserohilum turcicum in maize.
abstract: Eight potential biological control agents (BCAs) were evaluated in planta in order to assess their effectiveness in reducing disease severity of northern leaf blight caused by Exserohilum turcicum. The assay was carried out in greenhouse. Twenty-six-day-old plants, V4 phenological stage, were inoculated with antagonists by foliar spray. Only one biocontrol agent was used per treatment. Ten days after this procedure, all treatments were inoculated with E. turcicum by foliar application. Treatments performed were: C-Et: control of E. turcicum; T1: isolate 1 (Enterococcus genus)+E. turcicum; T2: isolate 2 (Corynebacterium genus)+E. turcicum; T3: isolate 3 (Pantoea genus)+E. turcicum; T4: isolate 4 (Corynebacterium genus)+E. turcicum; T5: isolate 5 (Pantoea genus)+E. turcicum; T6: isolate 6 (Bacillus genus)+E. turcicum; T7: isolate 7 (Bacillus genus)+E. turcicum; T8: isolate 8 (Bacillus genus)+E. turcicum. Monitoring of antagonists on the phyllosphere was performed at different times. Furthermore, the percentage of infected leaves and, plant and leaf incidence were determined. Foliar application of different bacteria significantly reduced the leaf blight between 30-78% and 39-56% at 20 and 39 days respectively. It was observed that in the V10 stage of maize plants, isolate 8 (Bacillus spp.) caused the greatest effect on reducing the severity of northern leaf blight. Moreover, isolate 8 was the potential BCA that showed more stability in the phyllosphere. At 39 days, all potential biocontrol agents had a significant effect on controlling the disease caused by E. turcicum.
authors.affiliation: Laboratorio de Ecología Microbiana, Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas Físico Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.
authors.forename: Melina
authors.initials: M
authors.lastname: Sartori
authors.affiliation: Laboratorio de Ecología Microbiana, Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas Físico Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.
authors.forename: Andrea
authors.initials: A
authors.lastname: Nesci
authors.affiliation: Oro Verde, Servicios fitosanitarios, Río Cuarto, Córdoba, Argentina.
authors.forename: Julián
authors.initials: J
authors.lastname: García
authors.affiliation: Laboratorio de Ecología Microbiana, Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas Físico Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.
authors.forename: María A
authors.initials: MA
authors.lastname: Passone
authors.affiliation: Laboratorio de Ecología Microbiana, Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas Físico Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.
authors.forename: Analía
authors.initials: A
authors.lastname: Montemarani
authors.affiliation: Laboratorio de Ecología Microbiana, Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas Físico Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina. Electronic address: metcheverry@exa.unrc.edu.ar.
authors.forename: Miriam
authors.initials: M
authors.lastname: Etcheverry
mesh_terms: D001203:Ascomycota; D001419:Bacteria; D061046:Biological Control Agents; D010935:Plant Diseases; D003313:Zea mays
chemical_list: D061046:Biological Control Agents
keywords: Bacterias filosféricas; Biological control; Control biológico; Estrategias de prevención; Exserohilum turcicum; Phyllospheric bacteria; Prevention strategy