title: Aedes-Borne Virus-Mosquito Interactions: Mass Spectrometry Strategies and Findings.
abstract: Aedes-borne viruses are responsible for high-impact neglected tropical diseases and unpredictable outbreaks such as the ongoing Zika epidemics. Aedes mosquitoes spread different arboviruses such as Dengue virus (DENV), Chikungunya virus (CHIKV), and Zika virus, among others, and are responsible for the continuous emergence and reemergence of these pathogens. These viruses have complex transmission cycles that include two hosts, namely the Aedes mosquito as a vector and susceptible vertebrate hosts. Human infection with arboviruses causes diseases that range from subclinical or mild to febrile diseases, encephalitis, and hemorrhagic fever. Infected mosquitoes do not show detectable signs of disease, even though the virus maintains a lifelong persistent infection. The infection of the Aedes mosquito by viruses involves a molecular crosstalk between cell and viral proteins. An understanding of how mosquito vectors and viruses interact is of fundamental interest, and it also offers novel perspectives for disease control. In recent years, mass spectrometry (MS)-based strategies in combination with bioinformatics have been successfully applied to identify and quantify global changes in cellular proteins, lipids, peptides, and metabolites in response to viral infection. Although the information about proteomics in the Aedes mosquito is limited, the information that has been reported can set up the basis for future studies. This review reflects how MS-based approaches have extended our understanding of Aedes mosquito biology and the development of DENV and CHIKV infection in the vector. Finally, this review discusses future challenges in the field.
authors.affiliation: 1 Laboratorio de Proteómica, Departamento de Infección e Inmunidad, Centro de Investigación sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, México.
authors.forename: Victoria
authors.initials: V
authors.lastname: Pando-Robles
authors.affiliation: 2 Laboratorio Universitario de Proteómica, Instituto de Biotecnología. Universidad Nacional Autónoma de México , Cuernavaca, México.
authors.forename: Cesar V
authors.initials: CV
authors.lastname: Batista
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D001102:Arbovirus Infections / Q000635:transmission* / Q000821:virology*; D001103:Arboviruses / Q000502:physiology*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*
keywords: Aedes aegypti; Chikungunya virus; Dengue virus; proteomics; vector biology