title: GATA3 expression in triple-negative breast cancers.
abstract: OBJECTIVE
  GATA-binding protein 3 (GATA3) is a well-studied transcription factor found to be essential in the development of luminal breast epithelium and has been identified in a variety of tumour types, including breast and urothelial carcinomas, making it a useful immunohistochemistry marker in the diagnosis of both primary and metastatic disease.
  
  
  RESULTS
  We investigated GATA3 protein expression in a 106 primary triple-negative breast carcinomas (100 basal-like, six non-basal-like) using Cell Marque mouse monoclonal anti-GATA3 (L50-823). Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to quantify mRNA expression in 22 triple-negative breast cancers (TNBCs) (20 primary and two cell lines), four luminal (three primary and one cell line) and five human epidermal growth factor receptor 2 (HER2) (four primary and one cell line) amplified tumours. In 98 TNBCs where IHC was assessable, 47 (48%) had a 1+ or greater staining with 20 (21%) having high GATA3 expression when using a weighted scoring.
  
  
  CONCLUSIONS
  Our study has demonstrated that GATA3 expression is common in primary triple-negative breast carcinomas. It also suggests that although GATA3 is an oestrogen receptor (ER) regulated gene, it still proves useful in differentiating between primary and metastatic tumours in patients with a history of breast cancer regardless of its molecular subtype.
authors.affiliation: Department of Pathology, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia.
authors.forename: David J
authors.identifier: http://orcid.org/0000-0002-9258-3252
authors.initials: DJ
authors.lastname: Byrne
authors.affiliation: Department of Pathology, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia.
authors.forename: Siddhartha
authors.initials: S
authors.lastname: Deb
authors.affiliation: Department of Pathology, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia.
authors.forename: Elena A
authors.initials: EA
authors.lastname: Takano
authors.affiliation: Department of Pathology, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia.|Department of Pathology, the University of Melbourne, Melbourne, Victoria, Australia.
authors.forename: Stephen B
authors.initials: SB
authors.lastname: Fox
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D005260:Female; D050990:GATA3 Transcription Factor / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D008875:Middle Aged; D064726:Triple Negative Breast Neoplasms / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D050990:GATA3 Transcription Factor; C494749:GATA3 protein, human
keywords: 
  TNBC
  ; GATA3; Immunohistochemistry; breast cancer