title: Development of a validated strategy for the determination of tryptamine in human cerebrospinal fluid in the presence of competitors using molecularly imprinted polymers.
abstract: This study presents a validated strategy for the determination of tryptamine in the presence of its competitors, which involves the molecularly imprinted solid-phase extraction combined with high-performance liquid chromatography coupled with fluorimetric detection. Tryptamine-imprinted microscale sorbent was produced from 4-vinylbenzoic acid and ethylene glycol dimethacrylate in methanol by precipitation polymerization, and its imprinting factor was equal to 15.4 in static experiments or 18.6 in dynamic binding experiments. The method for tryptamine determination in the presence of serotonin and l-tryptophan was validated using a complex matrix of bovine serum albumin yielding the recoveries of tryptamine that ranged between 98.7 and 107.0%. Very low limits of detection and limits of quantification for tryptamine (19.9 and 60.3 nmol/L, respectively) allow the quantification of tryptamine in human cerebrospinal fluid in the presence of tryptophan and serotonin.
authors.affiliation: Department of Organic Chemistry, Faculty of Pharmacy, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Luliński
authors.affiliation: Department of Toxicology, Faculty of Pharmacy, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Bamburowicz-Klimkowska
authors.affiliation: Department of Organic Chemistry, Faculty of Pharmacy, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Dana
authors.affiliation: Department of Organic Chemistry, Faculty of Pharmacy, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Maciejewska
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D054802:Molecular Imprinting; D011108:Polymers; D012701:Serotonin; D052616:Solid Phase Extraction; D014363:Tryptamines / Q000134:cerebrospinal fluid*; D014364:Tryptophan
chemical_list: D011108:Polymers; D014363:Tryptamines; D012701:Serotonin; D014364:Tryptophan
keywords: human cerebrospinal fluid; molecularly imprinted polymers; solid-phase extraction; tryptamine