title: Application of carbon nanosorbent for PRiME pass-through cleanup of 10 selected local anesthetic drugs in human plasma samples.
abstract: A novel PRiME (process, robustness, improvements, matrix effects, ease of use) pass-through cleanup procedure has been developed to improve the existing commercially available designs. Carbon nanosorbents, i.e., magnetic modified carboxyl-graphene (Mag-CG) and magnetic modified carboxyl-carbon nanotubes (Mag-CCNTs), have been synthesised and evaluated in PRiME pass-through cleanup procedure for human plasma prior to analysis of 10 selected local anesthetic drugs by liquid chromatography-tandem quadrupole mass spectrometry (LC-MS/MS). The matrix effect, an interesting phenomenon of ion suppression for local anesthetic drugs containing ester group and ion enhancement for other drugs containing acylamino group, has been minimized using carbon nanosorbents PRiME pass-through cleanup procedure. Under the optimal conditions, the obtained results show higher cleanup efficiency of the carbon nanosorbents with recoveries between 70.2% and 126%. Furthermore, the carbon nanosorbents are also evaluated for reuse up to 80-100 times. The limits of quantification (LOQs) for local anesthetic drugs are in the range of 0.024-0.15 μg/L. Validation results on linearity, specificity, accuracy, and precision, as well as the application to the analysis of lidocaine in five patients recruited from the lung cancer demonstrate the applicability to clinical studies.
authors.affiliation: Ningbo No.2 Hospital, Ningbo, Zhejiang, 315010, China.
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Ma
authors.affiliation: Department of Anesthesiology, Yinzhou People's Hospital, Medical School of Ningbo University, Ningbo, Zhejiang, 315040, China.
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Zhao
authors.affiliation: Ningbo No.2 Hospital, Ningbo, Zhejiang, 315010, China.
authors.forename: Qiu-Hong
authors.initials: QH
authors.lastname: Rui
authors.affiliation: Ningbo No.2 Hospital, Ningbo, Zhejiang, 315010, China.
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Liao
authors.affiliation: Ningbo No.2 Hospital, Ningbo, Zhejiang, 315010, China.
authors.forename: Yan-Min
authors.initials: YM
authors.lastname: Chen
authors.affiliation: Ningbo No.2 Hospital, Ningbo, Zhejiang, 315010, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Chemistry, Zhejiang University, Hangzhou, Zhejiang, 310027, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Chemistry, Zhejiang University, Hangzhou, Zhejiang, 310027, China; Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang, 315010, China. Electronic address: zhyg91213@163.com.
authors.forename: Yong-Gang
authors.initials: YG
authors.lastname: Zhao
mesh_terms: D000779:Anesthetics, Local / Q000097:blood* / Q000737:chemistry*; D001774:Blood Chemical Analysis / Q000379:methods*; D006108:Graphite / Q000737:chemistry; D006801:Humans; D059346:Magnets / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D000779:Anesthetics, Local; D037742:Nanotubes, Carbon; D006108:Graphite
keywords: Human plasma; Liquid chromatography-tandem quadrupole mass spectrometry (LC-MS/MS); Local anesthetic drugs; Magnetic modified carboxyl-carbon nanotubes (Mag-CCNTs); Magnetic modified carboxyl-graphene (Mag-CG); PRiME (process, robustness, improvements, matrix effects, ease of use) pass-through cleanup