title: Cloning, characterization and subcellular localization of Nuclear LIM interactor interacting factor gene from Leishmania donovani.
abstract: LIM domains are zinc-binding motifs that mediate protein-protein interactions and are found in a wide variety of cytoplasmic and nuclear proteins. The nuclear LIM domain family members have a number of different functions including transcription factors, gene regulation, cell fate determination, organization of the cytoskeleton and tumour formation exerting their function through various LIM domain interacting protein partners/cofactors. Nuclear LIM domain interacting proteins/factors have not been reported in any protozoan parasites including Leishmania. Here, we report for the first time cloning, characterization and subcellular localization of nuclear LIM interactor-interacting factor (NLI) like protein from Leishmania donovani, the causative agent of Indian Kala-azar. Primary sequence analysis of LdNLI revealed presence of characteristic features of nuclear LIM interactor-interacting factor. However, leishmanial NLI represents a distinct kinetoplastid group, clustered in a separate branch of the phylogenic tree. The sub-cellular distribution of LdNLI revealed the discreet localization in nucleus and kinetoplast only, suggesting that the gene may have a role in parasite gene expression.
authors.affiliation: Confocal Imaging, Sophisticated Instrumentation Facility, IIT Indore, Khandwa Road, Simrol, 453552, MP, India.
authors.forename: R
authors.initials: R
authors.lastname: Ravinder
authors.affiliation: Division of Biochemistry, CSIR-Central Drug Research Institute, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow, 226031, UP, India. Electronic address: neenacdri@yahoo.com.
authors.forename: N
authors.initials: N
authors.lastname: Goyal
mesh_terms: D000595:Amino Acid Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D018105:DNA, Kinetoplast / Q000378:metabolism; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D018628:Gene Dosage; D005786:Gene Expression Regulation; D060588:LIM Domain Proteins / Q000235:genetics / Q000378:metabolism*; D007893:Leishmania donovani / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D009687:Nuclear Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D015800:Protozoan Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D018105:DNA, Kinetoplast; D016054:DNA, Protozoan; D004268:DNA-Binding Proteins; D060588:LIM Domain Proteins; C000616962:NLI protein, Leishmania donovani; D009687:Nuclear Proteins; D015800:Protozoan Proteins
keywords: Expression; Immunofluorescence; Leishmania donovani; Nuclear LIM interactor-interacting factor; Phylogeny; Transfectant