title: Endodontic Irrigation Protocols: Effects on Bonding of Adhesive Systems to Coronal Enamel and Dentin.
abstract: OBJECTIVE
  To evaluate the influence of endodontic irrigation protocols on bond strength of total-etch and self-etch adhesive systems to coronal enamel and dentin.
  
  
  METHODS
  A total of 66 coronal slices from 11 permanent molars were assigned to six groups (n = 11): endodontic irrigation protocols (5% sodium hypochlorite + 17% EDTA; 2% chlorhexidine gel + saline solution + 17% EDTA or no solution - control) and adhesive systems (Adper Single Bond 2 or Clearfil SE Bond). Dental slices were exposed to endodontic irrigation protocols for 30-minute prior to the application of the adhesive systems. Starch tubes were placed over the enamel and the dentin surfaces of each slice (2-4 tubes in each substrate) and filled with Filtek Z350 XT (3M ESPE) to build the microshear bond strength (µSBS) specimens (0.72 mm2sectional area). After 24 hours of water storage (37°C), µSBS test was performed. Bond strength data (MPa) were analyzed by two-way ANOVA, separately for enamel and dentin (α = 0.05).
  
  
  RESULTS
  µSBS means were not influenced by endodontic irrigation protocols in enamel (p = 0.12) nor dentin (p = 0.49). Clearfil SE Bond system presented higher µSBS values than Adper Single Bond 2 both on enamel (p = 0.024) and dentin (p = 0.005).
  
  
  CONCLUSIONS
  Endodontic irrigation protocols (5% sodium hypochlorite or 2% chlorhexidine gel + saline solution combined with 17% EDTA) do not jeopardize the bond strength of adhesive systems to coronal enamel and dentin.
  
  
  CONCLUSIONS
  The results of this in vitro study suggest that endodontic irrigation protocols do not impair on bonding effectiveness of adhesive systems to coronal enamel and dentin. (J Esthet Restor Dent 29:222-228, 2017).
authors.affiliation: Post Graduate Program in Dental Science, Federal University of Santa Maria, Rua Marechal Floriano Peixoto, 1184, Santa Maria, RS, 97015-270, Brazil.
authors.forename: Marcos Paulo Marchiori
authors.initials: MPM
authors.lastname: Carvalho
authors.affiliation: Post Graduate Program in Dental Science, Federal University of Santa Maria, Rua Marechal Floriano Peixoto, 1184, Santa Maria, RS, 97015-270, Brazil.
authors.forename: Victor Hugo Carvalho
authors.initials: VHC
authors.lastname: Morari
authors.affiliation: Department of Restorative Dentistry, Federal University of Santa Maria, Rua Marechal Floriano Peixoto, 1184, Santa Maria, RS, 97015-270, Brazil.
authors.forename: Alexandre Henrique
authors.initials: AH
authors.lastname: Susin
authors.affiliation: Department of Stomatology, Federal University of Santa Maria, Rua Marechal Floriano Peixoto, 1184, Santa Maria, RS, 97015-270, Brazil.
authors.forename: Rachel De Oliveira
authors.identifier: http://orcid.org/0000-0001-7737-2257
authors.initials: RO
authors.lastname: Rocha
authors.affiliation: Department of Restorative Dentistry, Federal University of Santa Maria, Rua Marechal Floriano Peixoto, 1184, Santa Maria, RS, 97015-270, Brazil.
authors.forename: Luiz Felipe
authors.initials: LF
authors.lastname: Valandro
authors.affiliation: Department of Restorative Dentistry, Federal University of Santa Maria, Rua Marechal Floriano Peixoto, 1184, Santa Maria, RS, 97015-270, Brazil.
authors.forename: Fabio Zovico Maxnuck
authors.initials: FZM
authors.lastname: Soares
mesh_terms: D000134:Acid Etching, Dental; D002710:Chlorhexidine / Q000737:chemistry; D003188:Composite Resins; D001840:Dental Bonding; D003738:Dental Cements / Q000737:chemistry*; D003743:Dental Enamel / Q000737:chemistry*; D003804:Dentin / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D008963:Molar; D019279:Resin Cements; D012973:Sodium Hypochlorite / Q000737:chemistry; D013499:Surface Properties; D007507:Therapeutic Irrigation / Q000379:methods*
chemical_list: C554641:Adper single bond 2; C438313:Clearfil SE Bond; D003188:Composite Resins; D003738:Dental Cements; D019279:Resin Cements; C533204:filtek Z350; D012973:Sodium Hypochlorite; D002710:Chlorhexidine