title: Simultaneous Enhancement of Bioactivity and Stability of Laccase by Cu2+/PAA/PPEGA Matrix for Efficient Biosensing and Recyclable Decontamination of Pyrocatechol.
abstract: Simultaneously enhancing the catalytic bioactivity and stability of enzyme is still an intractable issue in the enzymatic study. Herein, a facile and effective approach was designed to immobilize and modify laccase on a Cu2+-adsorbed pyrene-terminated block copolymer [poly(acrylic acid)/poly(poly(ethylene glycol) acrylate)] (PAA/PPEGA), which was prepared via well-controlled reversible addition-fragmentation chain transfer polymerization. PAA provided the supporting matrix for firm immobilization of Cu2+, an enzyme bioactivity inducer, onto the microstructure of laccase, while avoiding any contamination of the heavy metal Cu2+into the following application system. The water-soluble, biocompatible, and nontoxic PPEGA was used as an ideal modifier to improve the laccase stability. Accordingly, the modified laccase exhibited enhanced catalytic bioactivity and stability simultaneously to 447% and 237%, respectively. The modified laccase was immobilized on the highly oriented pyrolytic graphite surface and large-area graphene papers through π-π stacking interactions between the pyrene moiety of PAA/PPEGA and the π-conjugated graphenelike surface. The as-prepared portable solid-state electrochemical laccase biosensor showed lowest detection limit of 50 nM (S/N ≥ 3) and long-term stability for pyrocatechol detection. Besides, the laccase immobilization on graphene paper provided efficient pyrocatechol decontamination platform with convenience and recyclability, which could retain the laccase bioactivity of 176% after 8 consecutive operations.
authors.affiliation: Center for Micro/Nano Luminescent and Electrochemical Materials, College of Materials Science and Engineering; Institute for Graphene Applied Technology Innovation; Laboratory of Fiber Materials and Modern Textiles, the Growing Base for State Key Laboratory; Collaborative Innovation Center for Marine Biomass Fibers Materials and Textiles of Shandong Province, Qingdao University , Qingdao 266071, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: Center for Micro/Nano Luminescent and Electrochemical Materials, College of Materials Science and Engineering; Institute for Graphene Applied Technology Innovation; Laboratory of Fiber Materials and Modern Textiles, the Growing Base for State Key Laboratory; Collaborative Innovation Center for Marine Biomass Fibers Materials and Textiles of Shandong Province, Qingdao University , Qingdao 266071, China.
authors.forename: Yuanhong
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Center for Micro/Nano Luminescent and Electrochemical Materials, College of Materials Science and Engineering; Institute for Graphene Applied Technology Innovation; Laboratory of Fiber Materials and Modern Textiles, the Growing Base for State Key Laboratory; Collaborative Innovation Center for Marine Biomass Fibers Materials and Textiles of Shandong Province, Qingdao University , Qingdao 266071, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Peng
authors.affiliation: Center for Micro/Nano Luminescent and Electrochemical Materials, College of Materials Science and Engineering; Institute for Graphene Applied Technology Innovation; Laboratory of Fiber Materials and Modern Textiles, the Growing Base for State Key Laboratory; Collaborative Innovation Center for Marine Biomass Fibers Materials and Textiles of Shandong Province, Qingdao University , Qingdao 266071, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Li
authors.affiliation: Center for Micro/Nano Luminescent and Electrochemical Materials, College of Materials Science and Engineering; Institute for Graphene Applied Technology Innovation; Laboratory of Fiber Materials and Modern Textiles, the Growing Base for State Key Laboratory; Collaborative Innovation Center for Marine Biomass Fibers Materials and Textiles of Shandong Province, Qingdao University , Qingdao 266071, China.
authors.forename: Jingquan
authors.identifier: 0000-0001-6178-8661
authors.initials: J
authors.lastname: Liu
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D015374:Biosensing Techniques; D002396:Catechols / Q000378:metabolism*; D003300:Copper / Q000378:metabolism*; D003666:Decontamination / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D042845:Laccase / Q000378:metabolism*; D057230:Limit of Detection; D046529:Microscopy, Electron, Transmission; D011092:Polyethylene Glycols / Q000737:chemistry*; D058105:Polymerization; D066244:Proton Magnetic Resonance Spectroscopy; D011721:Pyrenes / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000180:Acrylic Resins; D002396:Catechols; D011721:Pyrenes; D011092:Polyethylene Glycols; D003300:Copper; C030984:pyrene; D042845:Laccase; C034221:catechol