title: Environmental and biological determination of acrolein using new cold fiber solid phase microextraction with gas chromatography mass spectrometry.
abstract: Acrolein is a pollutant released daily to the indoor environment from different sources. The present study reports the development of a simple and sensitive cold fiber solid phase microextraction sampling method for the determination of acrolein in exhaled air and indoor air by gas chromatography mass spectrometry. O-(2,3,4,5,6-pentafluorobenzyl) hydroxylamine was used as derivatizing agent supported on a 65-μm polydimethylsiloxane-divinylbenzene SPME fiber. An acrolein permeation tube at 326.25 ng min-1rate was used to generate gaseous standards. The method shows good results for main validation parameters. The limits of detection and quantification were 2.88 and 5.08 μg m-3, respectively, for indoor analysis; and 2.40 and 3.79 μg m-3, respectively, for exhaled air analysis. The precision showed standard deviation ranges from 6.00 to 8.00% for intra-assay analyses and from 8.00 to 10.00% for inter-assay analyses. After optimizing the conditions, analyses of real samples were performed on indoor environments contaminated by cigarette smoke, or heated oil, including pastry shops, restaurants, churros stands, and closed parking cars located in the city of Belo Horizonte, Brazil. Acrolein breaths of exposed people were also determined. A good Pearson correlation coefficient (r = 0.901) was observed between the concentration of acrolein in indoor air and exhaled air, allowing to propose acrolein breath as environmental exposure biomarker. Graphical Abstract Cold fiber solid phase microextraction gas chromatography/mass spectrometry.
authors.affiliation: Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, 31270-901, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Cláudia M
authors.initials: CM
authors.lastname: Dias
authors.affiliation: Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, 31270-901, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Helvécio C
authors.initials: HC
authors.lastname: Menezes
authors.affiliation: Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, 31270-901, Belo Horizonte, Minas Gerais, Brazil. zenilda@ufmg.br.
authors.forename: Zenilda L
authors.initials: ZL
authors.lastname: Cardeal
mesh_terms: D000171:Acrolein / Q000032:analysis* / Q000737:chemistry; D016902:Air Pollution, Indoor / Q000032:analysis*; D001944:Breath Tests / Q000379:methods*; D004784:Environmental Monitoring / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008670:Metals / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052617:Solid Phase Microextraction / Q000379:methods*
chemical_list: D008670:Metals; D000171:Acrolein
keywords: Acrolein; Cold fiber SPME; Exhaled air; Indoor