title: Olive Oil-Supplemented Lipid Emulsion Induces CELF1 Expression and Promotes Apoptosis in Caco-2 Cells.
abstract: OBJECTIVE
  Parenterally-administered lipid emulsion (LE) is a key cause of enterocyte apoptosis under total parenteral nutrition, yet the pathogenesis has not been fully understood. CUGBP, Elav-like family member 1 (CELF1) has been recently identified as a crucial modulator of apoptosis, and thus this study sought to investigate its role in the LE-induced apoptosis in vitro.
  
  
  METHODS
  Caco-2 cells were used as an in vitro model. The cells were treated with varying LEs derived from soybean oil, olive oil or fish oil, and changes in the apoptosis and CELF1 expression were assessed. Rescue study was performed using transient knockdown of CELF1 with specific siRNA prior to LE treatment. Regulation of CELF1 by LE treatment was studied using quantitative real-time PCR and Western blotting.
  
  
  RESULTS
  All the LEs up-regulated CELF1expression and induced apoptosis, but only olive oil-supplemented lipid emulsion (OOLE)-induced apoptosis was attenuated by depletion of CELF1. Up-regulation of apoptosis-inducing factor (AIF) was involved in OOLE-induced CELF1 dependent apoptosis. The protein expression of CELF1 was up-regulated by OOLE in a dose- and time-dependent manner, but the mRNA expression of CELF1 was unchanged. Analysis by polysomal profiling and nascent protein synthesis revealed that the regulation of CELF1 by OOLE treatment was mediated by directly accelerating its protein translation.
  
  
  CONCLUSIONS
  OOLE-induces apoptosis in Caco-2 cells partially through up-regulation of CELF1.
authors.forename: Jun-Kai
authors.initials: JK
authors.lastname: Yan
authors.forename: Jie
authors.initials: J
authors.lastname: Zhu
authors.forename: Zi-Zhen
authors.initials: ZZ
authors.lastname: Gong
authors.forename: Jie
authors.initials: J
authors.lastname: Wen
authors.forename: Yong-Tao
authors.initials: YT
authors.lastname: Xiao
authors.forename: Wei
authors.initials: W
authors.lastname: Cai
authors.forename: Tian
authors.initials: T
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D051033:Apoptosis Inducing Factor / Q000378:metabolism; D000067879:CELF1 Protein / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D018938:Caco-2 Cells; D053148:Caspase 3 / Q000378:metabolism; D053179:Caspase 7 / Q000378:metabolism; D004655:Emulsions / Q000737:chemistry* / Q000494:pharmacology; D005395:Fish Oils / Q000737:chemistry; D006801:Humans; D000069463:Olive Oil / Q000737:chemistry / Q000494:pharmacology*; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D013024:Soybean Oil / Q000737:chemistry; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D051033:Apoptosis Inducing Factor; D000067879:CELF1 Protein; D004655:Emulsions; D005395:Fish Oils; D000069463:Olive Oil; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D013024:Soybean Oil; D053148:Caspase 3; D053179:Caspase 7
keywords: Apoptosis; CELF1; Caco-2; Olive oil-supplemented lipid emulsion; Parenteral nutrition