title: Comprehensive and Quantitative Analysis of Polyphosphoinositide Species by Shotgun Lipidomics Revealed Their Alterations in db/db Mouse Brain.
abstract: Polyphosphoinositides (PPI) play crucial roles in cellular signaling and functions. However, comprehensively determining the changed levels of these species during different cellular processes has faced difficulties. Herein, we applied a novel methylation pattern recognition and simulation approach, and we exploited newly derived fragmentation patterns of methylated PPI species for comprehensive analysis of PPI species including phosphate position(s) and fatty acyl chains capable of circumpassing previous limitations. The developed method was applied for quantitative analysis of PPI species present in diabetic mouse cortex and liver, and it allowed us to unravel the marked reduction of PPI levels in brain cortices of db/db mice for the first time. Taken together, we developed a powerful and high-throughput method for comprehensive analysis of PPI species, which should greatly contribute to the elucidation of PPI biology under different disease states.
authors.affiliation: Center for Metabolic Origins of Disease, Sanford Burnham Prebys Medical Discovery Institute , Orlando, Florida 32827, United States.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Wang
authors.affiliation: Center for Metabolic Origins of Disease, Sanford Burnham Prebys Medical Discovery Institute , Orlando, Florida 32827, United States.
authors.forename: Juan Pablo
authors.initials: JP
authors.lastname: Palavicini
authors.affiliation: Center for Metabolic Origins of Disease, Sanford Burnham Prebys Medical Discovery Institute , Orlando, Florida 32827, United States.
authors.forename: Miao
authors.initials: M
authors.lastname: Wang
authors.affiliation: Center for Metabolic Origins of Disease, Sanford Burnham Prebys Medical Discovery Institute , Orlando, Florida 32827, United States.
authors.forename: Linyuan
authors.initials: L
authors.lastname: Chen
authors.affiliation: Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine , St. Louis, Missouri 63110, United States.
authors.forename: Kui
authors.initials: K
authors.lastname: Yang
authors.affiliation: Center for Metabolic Origins of Disease, Sanford Burnham Prebys Medical Discovery Institute , Orlando, Florida 32827, United States.
authors.forename: Peter A
authors.initials: PA
authors.lastname: Crawford
authors.affiliation: Center for Metabolic Origins of Disease, Sanford Burnham Prebys Medical Discovery Institute , Orlando, Florida 32827, United States.
authors.forename: Xianlin
authors.initials: X
authors.lastname: Han
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001923:Brain Chemistry; D003920:Diabetes Mellitus / Q000378:metabolism* / Q000473:pathology; D007536:Isomerism; D008099:Liver / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D055432:Metabolomics / Q000379:methods; D008745:Methylation; D051379:Mice; D008810:Mice, Inbred C57BL; D018129:Phosphatidylinositol Phosphates / Q000032:analysis / Q000378:metabolism*
chemical_list: D018129:Phosphatidylinositol Phosphates