title: Sex hormone binding globulin: Expression throughout early development and adult pejerrey fish, Odontesthes bonariensis.
abstract: Sex hormone binding globulin (Shbg) is a plasma glycoprotein that binds and transports steroids in the blood of all vertebrate classes apart from birds. In the present study we characterized shbg from pejerrey, a fish species with a well characterized temperature-dependent sex determination. The pejerrey shbg mRNA comprises 1185bp encoding for a 395 amino acid Shbg precursor protein that includes a leader sequence for secretion. Relative quantification of shbg transcript abundance revealed expression early in development coinciding with the sex-determining period and probably in association with temperature leading to male determination. The hepatopancreas was the main site of shbg expression, which varied according to the sex cycle in females. It was also expressed in gills, gonads, gut and taste buds during both larval stages and in adult fish. The presence of Shbg in organs in close contact with the environment such as gills, pseudobranchs, gut and taste buds suggests that these are potential sources of uptake or release of steroids/xenosteroids to and from the aquatic environment.
authors.affiliation: Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECH), CONICET-UNSAM, Chascomús, Buenos Aires, Argentina.
authors.forename: Anelisa
authors.initials: A
authors.lastname: González
authors.affiliation: Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECH), CONICET-UNSAM, Chascomús, Buenos Aires, Argentina.
authors.forename: Juan I
authors.initials: JI
authors.lastname: Fernandino
authors.affiliation: Department of Cellular and Physiological Sciences, Faculty of Medicine, The University of British Columbia, British Columbia, Canada.
authors.forename: Geoffrey L
authors.initials: GL
authors.lastname: Hammond
authors.affiliation: Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECH), CONICET-UNSAM, Chascomús, Buenos Aires, Argentina. Electronic address: somoza@intech.gov.ar.
authors.forename: Gustavo M
authors.initials: GM
authors.lastname: Somoza
mesh_terms: D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D007814:Larva / Q000235:genetics / Q000254:growth & development; D008297:Male; D016366:Open Reading Frames / Q000235:genetics; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012733:Sex Differentiation / Q000235:genetics; D012738:Sex Hormone-Binding Globulin / Q000235:genetics* / Q000378:metabolism; D023701:Smegmamorpha / Q000235:genetics* / Q000254:growth & development*; D013696:Temperature
chemical_list: D012333:RNA, Messenger; D012738:Sex Hormone-Binding Globulin
keywords: Early development; Fish gills; Pejerrey; Sex hormone binding globulin; Sex steroids; Taste buds