title: Intracellular Assessment of ATP Levels inCaenorhabditis elegans.
abstract: Eukaryotic cells heavily depend on adenosine triphosphate (ATP) generated by oxidative phosphorylation (OXPHOS) within mitochondria. ATP is the major energy currency molecule, which fuels cell to carry out numerous processes, including growth, differentiation, transportation and cell death among others (Khakh and Burnstock, 2009). Therefore, ATP levels can serve as a metabolic gauge for cellular homeostasis and survival (Artal-Sanz and Tavernarakis, 2009; Gomeset al., 2011; Palikaraset al., 2015). In this protocol, we describe a method for the determination of intracellular ATP levels using a bioluminescence approach in the nematodeCaenorhabditis elegans.
authors.affiliation: Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Crete, Greece.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Palikaras
authors.affiliation: Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Crete, Greece; Department of Basic Sciences, Faculty of Medicine, University of Crete, Crete, Greece.
authors.forename: Nektarios
authors.initials: N
authors.lastname: Tavernarakis
keywords: ATP; Ageing; Caenorhabditis elegans; Energy homeostasis; Luciferase; Metabolism; Mitochondria