title: On-Line Screening, Isolation and Identification of Antioxidant Compounds of Helianthemum ruficomum.
abstract: ManyHelianthemumspecies (Cistaceae) are recognized for their various medicinal virtues.Helianthemum ruficomumis an endemic species to the septentrional Sahara on which no report is available so far. The purpose of this work was to investigate the chemical composition and the radical scavenging capacity of this species and its isolated components. Collected from Mougheul (south-west of Algeria), the aerial parts were macerated with 80% EtOH/H₂O, after evaporation, the remaining extract was diluted with H₂O and extracted with petroleum ether, chloroform, ethyl acetate andn-butanol. EtOAc andn-BuOH extracts were evaluated for their free radical scavenging capacity by on-line HPLC-ABTS•+assay. The obtained data which were confirmed by TEAC and ORAC assays, allowed guiding the fractionation of these extracts by CC, TLC and reverse phase HPLC. Among the components, 14 were isolated and identified by spectroscopic analyses: protocatechuic acid (1),trans-tiliroside (2),cis-tiliroside (3), astragalin (4), picein (7), vanillic acid 4-O-β-d-glucopyranoside (8), lavandoside (9), 4-hydroxybenzoic acid 4-O-β-d-glucopyranoside (10), nicotiflorin (11), rutin (12), vicenin-2(13), narcissin (14) and stigmasterol (5) and β-sitosterol (6) as a mixture (71% and 29%, respectively). Compounds5,7,8,9,10and14were new for the genusHelianthemum. The antioxidant power of all the isolated compounds was also evaluated by HPLC-ABTS•+, TEAC and ORAC assays. The results clearly indicated high antioxidant potential of the extracts and tested compounds of this species especially, compounds1,4,8,9,10and12.
authors.affiliation: Unité de Recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques, Université des Frères Mentouri, Constantine 1, Route d'Aïn El Bey, 25000 Constantine, Algeria. ch.jasmin@hotmail.fr.
authors.forename: Yasmine
authors.initials: Y
authors.lastname: Chemam
authors.affiliation: Unité de Recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques, Université des Frères Mentouri, Constantine 1, Route d'Aïn El Bey, 25000 Constantine, Algeria. sbenayache@yahoo.com.
authors.forename: Samir
authors.initials: S
authors.lastname: Benayache
authors.affiliation: Chimie Analytique des Molécules Bioactives, Institut Pluridisciplinaire Hubert Curien (UMR 7178 CNRS/UDS), 74 route du Rhin, 67400 Illkirch, France. Eric.Marchioni@unistra.fr.
authors.forename: Eric
authors.initials: E
authors.lastname: Marchioni
authors.affiliation: Chimie Analytique des Molécules Bioactives, Institut Pluridisciplinaire Hubert Curien (UMR 7178 CNRS/UDS), 74 route du Rhin, 67400 Illkirch, France. minjzhao@unistra.fr.
authors.forename: Minjie
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Institut des Sciences Chimiques de Rennes, CNRS UMR 6226, Université de Rennes 1, 263 Avenue du Général Leclerc, CS 74205, 35042 Rennes CEDEX, France. paul.mosset.1@univ-rennes1.fr.
authors.forename: Paul
authors.initials: P
authors.lastname: Mosset
authors.affiliation: Unité de Recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques, Université des Frères Mentouri, Constantine 1, Route d'Aïn El Bey, 25000 Constantine, Algeria. fbenayache@yahoo.fr.
authors.forename: Fadila
authors.initials: F
authors.lastname: Benayache
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D030601:Cistaceae / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D015394:Molecular Structure; D064209:Phytochemicals / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D064209:Phytochemicals; D010936:Plant Extracts
keywords: Cistaceae; HPLC-ABTS•+; Helianthemum ruficomum; ORAC; TEAC; antioxidant activity