title: Characterization of a new fungal immunomodulatory protein, FIP-dsq2 from Dichomitus squalens.
abstract: FIP-dsq2, a new immunomodulatory protein, was identified in Basidiomycota Dichomitus squalens by gene mining. FIP-dsq2 contained 111 amino acids with a molecular weight of 12.51kDa. FIP-dsq2 had a homology range of 51-65% to the reported FIPs. The predicted 3-dimensional model had more similar identical folding patterns in LZ-8 than for FIP-fve. Evolutionary analysis indicated substantial phylogenetic differences were existed with the other FIPs. Overexpression of a 14.07kDa soluble recombinant FIP-dsq2 (rFIP-dsq2) was achieved in Rosetta (pGEX-6P-1) and the purified recombinant protein was homodimer verified by gel filtration chromatography analysis. Antitumour ability of rFIP-dsq2 to human lung adenocarcinoma A549 cells was between LZ-8 and FIP-fve. The cytotoxic effect of rFIP-dsq2 in A549 cancer cells was dose-dependent and the half-maximal inhibitory concentration (IC50) was 15.08μg/mL. Furthermore, rFIP-dsq2 at 8μg/mL could significantly induce apoptosis and interrupt migration in A549 cells. In addition, the antitumour-mechanism exploration suggested that rFIP-dsq2 inhibited A549 proliferation uniquely via apoptotic cell death pathway. The results stated that rFIP-dsq2 was a promising candidate for use in future lung cancer therapy.
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuan Ming Yuan West Road, Beijing 100193, China. Electronic address: lishuying2000@163.com.
authors.forename: Shuying
authors.initials: S
authors.lastname: Li
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuan Ming Yuan West Road, Beijing 100193, China.
authors.forename: Zhonghao
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuan Ming Yuan West Road, Beijing 100193, China.
authors.forename: Lichao
authors.initials: L
authors.lastname: Sun
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuan Ming Yuan West Road, Beijing 100193, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuan Ming Yuan West Road, Beijing 100193, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuan Ming Yuan West Road, Beijing 100193, China. Electronic address: wangfengzhong@sina.com.
authors.forename: Fengzhong
authors.initials: F
authors.lastname: Wang
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuan Ming Yuan West Road, Beijing 100193, China. Electronic address: 2002hongzhi30@163.com.
authors.forename: Fengjiao
authors.initials: F
authors.lastname: Xin
mesh_terms: D000072283:A549 Cells; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D001487:Basidiomycota / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D008958:Models, Molecular; D008970:Molecular Weight; D010802:Phylogeny; D017510:Protein Folding; D020539:Sequence Analysis, Protein / Q000379:methods*; D017386:Sequence Homology, Amino Acid
chemical_list: D000970:Antineoplastic Agents; D005656:Fungal Proteins
keywords: Apoptosis; Fungal immunomodulatory protein; Migration; NSCLC; Proliferation