title: Capillary Microfluidics-Assembled Virus-like Particle Bionanoreceptor Interfaces for Label-Free Biosensing.
abstract: A capillary microfluidics-integrated sensor system is developed for rapid assembly of bionanoreceptor interfaces on-chip and label-free biosensing. Genetically engineered Tobacco mosaic virus (TMV) virus-like particles (VLPs), displaying thousands copies of identical receptor peptides FLAG-tags, are utilized as nanoceptors for antibody sensing. Controlled and accelerated assembly of VLP receptor layer on impedance sensor has been achieved using capillary action and surface evaporation from an open-channel capillary microfluidic system. VLPs create a dense and localized receptor monolayer on the impedance sensor using only 5 μL of VLP sample solution (0.2 mg/mL) in only 6 min at room temperature. The VLP-functionalized impedance sensor is capable of label-free detection of target antibodies down to 55 pM concentration within 5 min. These results highlight the significant potentials of an integrated microsystem for rapid and controlled receptor-transducer interface creation and the nanoscale VLP-based sensors for fast, accurate, and decentralized pathogen detection.
authors.affiliation: Department of Electrical Engineering, Princeton University , Princeton, New Jersey 08544, United States.
authors.forename: Faheng
authors.identifier: http://orcid.org/0000-0002-1173-6553
authors.initials: F
authors.lastname: Zang
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Gerasopoulos
authors.forename: Adam D
authors.initials: AD
authors.lastname: Brown
authors.forename: James N
authors.initials: JN
authors.lastname: Culver
authors.forename: Reza
authors.initials: R
authors.lastname: Ghodssi
mesh_terms: D000906:Antibodies; D015374:Biosensing Techniques; D017097:Electric Impedance; D044085:Microfluidics; D014027:Tobacco Mosaic Virus
chemical_list: D000906:Antibodies
keywords: biosensor; capillary microfluidics; label-free; nanoreceptor; virus-like particle