title: Antiviral effects of extracts from Celosia cristata and Raphanus sativus roots against viral hemorrhagic septicemia virus.
abstract: The antiviral activity of an extract mixture from Celosia cristata and Raphanus sativus was tested against viral hemorrhagic septicemia virus (VHSV). Pretreatment of EPC cells with this extract up to 72 h before VHSV infection markedly reduced the virus titer, but it had no effect when added after virus inoculation. In olive flounder that received 5 µg of extract per fish, Mx expression peaked at 48 h after treatment. In contrast, ISG15 and TLR2 expression peaked at 72 h, and that of TLR7 peaked at 48 h, followed by a slight decrease at 72 h, indicating that the antiviral activity was mediated by induction of gene expression involved in the innate immune response.
authors.affiliation: Department of Microbiology, Pukyong National University, 45, Yongso-ro, Nam-Gu, Busan, 48513, Republic of Korea.|Biotechnology Research Division, NIFS, Busan, 46083, Republic of Korea.
authors.forename: Yeon Jung
authors.initials: YJ
authors.lastname: Park
authors.affiliation: Gueulri Research Center, 410, Business Incubator Center, Hanyang University, Ansan, 15588, Republic of Korea.
authors.forename: Chiung
authors.initials: C
authors.lastname: Moon
authors.affiliation: Biotechnology Research Division, NIFS, Busan, 46083, Republic of Korea.
authors.forename: Jung-Ha
authors.initials: JH
authors.lastname: Kang
authors.affiliation: Department of Microbiology, Pukyong National University, 45, Yongso-ro, Nam-Gu, Busan, 48513, Republic of Korea. choitj@pknu.ac.kr.
authors.forename: Tae-Jin
authors.initials: TJ
authors.lastname: Choi
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D034762:Celosia / Q000737:chemistry*; D005393:Fish Diseases / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D005432:Flounder / Q000821:virology; D031941:Hemorrhagic Septicemia, Viral / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D029141:Novirhabdovirus / Q000187:drug effects* / Q000502:physiology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D031224:Raphanus / Q000737:chemistry*
chemical_list: D000998:Antiviral Agents; D029941:Fish Proteins; D010936:Plant Extracts
keywords: Cockscomb; Mx; Radish; TLR7; VHSV