title: Isolation of Pseudomonas fluorescens species highly resistant to pentachlorobenzene.
abstract: Polychlorinated aromatic compounds, including pentachlorobenzenes and hexachlorobenzenes, are recalcitrant industrial pollutants that cause adverse effects on living cells. In this paper, the isolation of Pseudomonas fluorescens species with high resistance to pentachlorobenzene (PeCB) is reported. It was found that, in contrast to its slightly negative effect on P. fluorescens growth, PeCB readily inhibited the cell growth of Serratia spp. and Escherichia coli strains, thus indicating that inhibition of bacterial growth by PeCB is species-dependent. Analysis of a P. fluorescens isolate revealed that the exposure to PeCB induced production of reactive oxygen species and led to an increase in the level of alkyl hydroperoxide reductase C (AhpC), an important enzyme enhancing the cell tolerance to organic hydroperoxides usually accumulated under oxidative stress. The putative mechanism conferring PeCB resistance to P. fluorescens and the potential use of P. fluorescens in bioremediation are discussed.
authors.affiliation: Department of Immunology, Microbiology and Parasitology, University of the Basque Country UPV/EHU, Leioa, Spain.
authors.forename: Itxaso
authors.initials: I
authors.lastname: Montánchez
authors.affiliation: Department of Biochemistry and Molecular Biology, University of the Basque Country (UPV/EHU), P.O. Box 644, 48080, Bilbao, Spain.
authors.forename: Anna Chao
authors.initials: AC
authors.lastname: Kaberdina
authors.affiliation: Department of Immunology, Microbiology and Parasitology, University of the Basque Country UPV/EHU, Leioa, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Sevillano
authors.affiliation: Department of Immunology, Microbiology and Parasitology, University of the Basque Country UPV/EHU, Leioa, Spain.
authors.forename: Lucía
authors.initials: L
authors.lastname: Gallego
authors.affiliation: Unit of Environmental Engineering, Ceit-IK4, Paseo Manuel de Lardizábal 15, 20018, San Sebastian, Spain.|IKERBASQUE, Basque Foundation for Science, Maria Diaz de Haro 3, 48013, Bilbao, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Rodríguez-Couto
authors.affiliation: Department of Immunology, Microbiology and Parasitology, University of the Basque Country UPV/EHU, Leioa, Spain. vladimir.kaberdin@ehu.eus.|IKERBASQUE, Basque Foundation for Science, Maria Diaz de Haro 3, 48013, Bilbao, Spain. vladimir.kaberdin@ehu.eus.|Research Centre for Experimental Marine Biology and Biotechnology (PIE-UPV/EHU), 48620, Plentzia, Spain. vladimir.kaberdin@ehu.eus.
authors.forename: Vladimir R
authors.initials: VR
authors.lastname: Kaberdin
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002722:Chlorobenzenes / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D018384:Oxidative Stress; D011551:Pseudomonas fluorescens / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D002722:Chlorobenzenes; D017382:Reactive Oxygen Species; C003060:pentachlorobenzene
keywords: Alkyl hydroperoxide reductase C; Cell adaptation; Oxidative stress; Polychlorinated benzenes; Reactive oxygen species (ROS); Toxic compounds