title: Distinct susceptibility and applicability of MDCK derivatives for influenza virus research.
abstract: Madin-Darby Canine Kidney (MDCK) cells are widely utilized as a substrate for influenza virus isolation and propagation due to the high yields of virus. Here we compared the conventional MDCK cell line, MDCK-SIAT1 and MDCK-London for viral production, cell survival, and suitability in testing antivirals using six influenza strains including two H1N1 (pandemic and epidemic strains), three H3N2 and one influenza B strain. Overall our results suggest that MDCK-London cell line is superior for virus culturing and quantification, and hence an ideal platform to evaluate antiviral drug efficacy against multiple strains of influenza. Our data also suggests that while virus titers determined by the hemagglutination assay (HA) and neuraminidase activity (NA) are widely used to indicate viral load, there is a poor correlation between these measurements and the infectious titer obtained by plaque assay.
authors.affiliation: Program in Medical Biotechnology, National Chung Hsing University, Taichung, Taiwan.|Center for Infectious Diseases, SRI International, Harrisonburg, Virginia, United States of America.
authors.forename: Shih-Chao
authors.initials: SC
authors.lastname: Lin
authors.affiliation: Center for Infectious Diseases, SRI International, Harrisonburg, Virginia, United States of America.
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Kappes
authors.affiliation: Center for Infectious Diseases, SRI International, Harrisonburg, Virginia, United States of America.
authors.forename: Mei-Chun
authors.initials: MC
authors.lastname: Chen
authors.affiliation: Department of Biomedical Sciences, National Chung Hsing University, Taichung, Taiwan.|Department of Biotechnology, Asia University, Taichung, Taiwan.|Department of Medical Research, China Medical University Hospital, Taichung, Taiwan.
authors.forename: Chi-Chen
authors.initials: CC
authors.lastname: Lin
authors.affiliation: Center for Infectious Diseases, SRI International, Harrisonburg, Virginia, United States of America.
authors.forename: Tony T
authors.initials: TT
authors.lastname: Wang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004285:Dogs; D006386:Hemagglutination Tests / Q000379:methods*; D006387:Hemagglutination, Viral; D009980:Influenza A virus / Q000502:physiology*; D061985:Madin Darby Canine Kidney Cells / Q000166:cytology* / Q000821:virology*; D009976:Orthomyxoviridae Infections / Q000821:virology*