title: TNF-α (-308G>A) and TNF-R1 (36A>G) single nucleotide polymorphisms are strong risk factors for odontogenic keratocystic tumor development.
abstract: BACKGROUND
  Polymorphisms in genes encoding tumor necrosis factor-α (TNF-α) and its receptor TNF-R1 have been shown to affect one person's susceptibility to develop certain neoplastic diseases. The aim of the present association study was to investigate whether single nucleotide polymorphisms (SNPs) in TNF-α (-308G>A) and TNF-R1 (36A>G) genes modulate the susceptibility for keratocystic odontogenic tumors (KCOTs) development in Serbian patients.
  
  
  METHODS
  Genotyping was performed in 60 KCOT patients and 125 healthy individuals, using polymerase chain reaction/restriction fragment length polymorphism analysis.
  
  
  RESULTS
  A significant difference in genotype and allele frequencies was found between patients and controls for both SNPs (P < 0.05). Carriers of the TNF-α A variant had an eightfold increase of KCOT risk (OR = 8.12, 95% CI = 3.98-16.56, P < 0.0001), while carriers of the TNF-R1 G variant had approximately a fourfold increase of KCOT risk (OR=3.65, CI: 1.60-8.40, P = 0.001).
  
  
  CONCLUSIONS
  Our findings suggest that the two polymorphisms are strong risk factors for KCOT development in Serbian population.
authors.affiliation: Clinic for Oral Surgery, School of Dental Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: Branislav
authors.initials: B
authors.lastname: Ilic
authors.affiliation: Department of Human Genetics, School of Dental Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: Nadja
authors.initials: N
authors.lastname: Nikolic
authors.affiliation: Clinic for Oral Surgery, School of Dental Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Andric
authors.affiliation: Clinic for Maxillofacial Surgery, School of Dental Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: Drago
authors.initials: D
authors.lastname: Jelovac
authors.affiliation: Department of Statistics, School of Dental Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: Biljana
authors.initials: B
authors.lastname: Milicic
authors.affiliation: School of Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: Tanja
authors.initials: T
authors.lastname: Jozic
authors.affiliation: School of Medicine, University of Belgrade, Belgrade, Serbia.|Clinic for Emergency Surgery, Emergency Center, Clinical Centre of Serbia, Belgrade, Serbia.
authors.forename: Slobodan
authors.initials: S
authors.lastname: Krstic
authors.affiliation: Department of Human Genetics, School of Dental Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: Jelena
authors.identifier: http://orcid.org/0000-0002-6225-7210
authors.initials: J
authors.lastname: Milasin
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007573:Jaw Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009808:Odontogenic Tumors / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000235:genetics*; D012307:Risk Factors; D055771:Serbia / Q000453:epidemiology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D055815:Young Adult
chemical_list: D047888:Receptors, Tumor Necrosis Factor, Type I; D014409:Tumor Necrosis Factor-alpha
keywords: 
  tumor necrosis factor-α
  ; TNFR1; odontogenic tumors; risk factors; single nucleotide polymorphisms