title: Activation and Differentiation of Mesenchymal Stem Cells.
abstract: Mesenchymal stem cells (MSCs) are multipotent cells and exhibit two main characteristics that define stem cells: self-renewal and differentiation. MSCs can migrate to sites of injury, inflammation, and tumor. Moreover, MSCs undergo myofibroblast like differentiation, including increased production of α-SMA in response to transforming growth factor-β (TGF-β), a growth factor commonly secreted by tumor cells to evade immune surveillance. Based on our previous finding hMSCs become activated and resemble carcinoma-associated myofibroblasts upon prolonged exposure to conditioned medium from MDAMB231 human breast cancer cells. Here, we show that keratinocyte conditioned medium (KCM) induces differentiation of MSCs to resemble dermal myofibroblast like cells using immunofluorescence techniques demonstrating punctate vinculin staining, and F-actin filaments.
authors.affiliation: Department of Pharmacology, Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey, 675 Hoes Lane West, Room 561, Piscataway, NJ, 08854, USA.
authors.forename: Pravin J
authors.initials: PJ
authors.lastname: Mishra
authors.affiliation: Department of Pharmacology, Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey, 675 Hoes Lane West, Room 561, Piscataway, NJ, 08854, USA. banerjed@rwjms.rutgers.edu.
authors.forename: Debabrata
authors.initials: D
authors.lastname: Banerjee
mesh_terms: D015415:Biomarkers; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002465:Cell Movement; D017077:Culture Media, Conditioned / Q000494:pharmacology; D020405:Dermis / Q000166:cytology; D005455:Fluorescent Antibody Technique; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D058628:Myofibroblasts / Q000166:cytology / Q000378:metabolism
chemical_list: D015415:Biomarkers; D017077:Culture Media, Conditioned
keywords: Breast cancer; Cell differentiation; Colorectal cancer; Dermal myofibroblast; Immunofluorescence; Mesenchymal stem cells; Migration; Myofibroblast; SDF-1; Stem cells