title: Expression of cyanobacterial FBP/SBPase in soybean prevents yield depression under future climate conditions.
abstract: Predictions suggest that current crop production needs to double by 2050 to meet global food and energy demands. Based on theory and experimental studies, overexpression of the photosynthetic enzyme sedoheptulose-1,7-bisphosphatase (SBPase) is expected to enhance C3 crop photosynthesis and yields. Here we test how expression of the cyanobacterial, bifunctional fructose-1,6/sedoheptulose-1,7-bisphosphatase (FBP/SBPase) affects carbon assimilation and seed yield (SY) in a major crop (soybean, Glycine max). For three growing seasons, wild-type (WT) and FBP/SBPase-expressing (FS) plants were grown in the field under ambient (400 μmol mol−1) and elevated (600 μmol mol−1) CO2 concentrations [CO2] and under ambient and elevated temperatures (+2.7 °C during daytime, +3.4 °C at night) at the SoyFACE research site. Across treatments, FS plants had significantly higher carbon assimilation (4–14%), Vc,max (5–8%), and Jmax (4–8%). Under ambient [CO2], elevated temperature led to significant reductions of SY of both genotypes by 19–31%. However, under elevated [CO2] and elevated temperature, FS plants maintained SY levels, while the WT showed significant reductions between 11% and 22% compared with plants under elevated [CO2] alone. These results show that the manipulation of the photosynthetic carbon reduction cycle can mitigate the effects of future high CO2 and high temperature environments on soybean yield.
authors.affiliation: Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.|Global Change and Photosynthesis Research Unit, Agricultural Research Service, United States Department of Agriculture, Urbana, IL, USA
authors.forename: Iris H
authors.initials: IH
authors.lastname: Köhler
authors.affiliation: Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
authors.forename: Ursula M
authors.initials: UM
authors.lastname: Ruiz-Vera
authors.affiliation: Department of Agronomy, Iowa State University, Ames, IA, USA.
authors.forename: Andy
authors.initials: A
authors.lastname: VanLoocke
authors.affiliation: Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.|Global Change and Photosynthesis Research Unit, Agricultural Research Service, United States Department of Agriculture, Urbana, IL, USA
authors.forename: Michell L
authors.initials: ML
authors.lastname: Thomey
authors.affiliation: Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, USA.
authors.forename: Tom
authors.initials: T
authors.lastname: Clemente
authors.affiliation: Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, USA.
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Long
authors.affiliation: Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.|Global Change and Photosynthesis Research Unit, Agricultural Research Service, United States Department of Agriculture, Urbana, IL, USA|Departments of Plant Biology and Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, USA
authors.forename: Donald R
authors.initials: DR
authors.lastname: Ort
authors.affiliation: Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.|Global Change and Photosynthesis Research Unit, Agricultural Research Service, United States Department of Agriculture, Urbana, IL, USA|Departments of Plant Biology and Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, USA
authors.forename: Carl J
authors.initials: CJ
authors.lastname: Bernacchi
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002244:Carbon / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D057231:Climate Change; D006358:Hot Temperature; D007087:Illinois; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000382:microbiology; D013025:Glycine max / Q000235:genetics / Q000254:growth & development* / Q000382:microbiology; D046940:Synechococcus / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002245:Carbon Dioxide; D002244:Carbon
keywords: Elevated CO2; elevated temperature; free air CO2 enrichment; Glycine max; sedoheptulose-1,7-bisphosphatase; Soy-T-FACE