title: Live-imaging analysis of germ cell proliferation in the C. elegans adult supports a stochastic model for stem cell proliferation.
abstract: The C. elegans adult hermaphrodite contains a renewable pool of mitotically dividing germ cells that are contained within the progenitor zone (PZ), at the distal region of the germline. From the PZ, cells enter meiosis and differentiate, ensuring the continued production of oocytes. In this study, we investigated the proliferation strategy used to maintain the PZ pool by using a photoconvertible marker to follow the fate of selected germ cells and their descendants in live worms. We found that the most distal pool of 6-8 rows of cells in the PZ (the distal third) behave similarly, with a fold expansion corresponding to one cell division every 6h on average. Proximal to this region, proliferation decreases, and by the proximal third of the PZ, most cells have stopped dividing. In addition, we show that all the descendants of cells in rows 3 and above move proximally and leave the PZ over time. Combining our data with previous studies, we propose a stochastic model for the C. elegans PZ proliferation, where a pool of proliferating stem cells divide symmetrically within the distal most 6-8 rows of the germline and exit from this stem cell niche occurs by displacement due to competition for limited space.
authors.affiliation: The Laboratory of Cell and Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address: simona.rosu@nih.gov.
authors.forename: Simona
authors.initials: S
authors.lastname: Rosu
authors.affiliation: The Laboratory of Cell and Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address: ornac@niddk.nih.gov.
authors.forename: Orna
authors.initials: O
authors.lastname: Cohen-Fix
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D017173:Caenorhabditis elegans / Q000166:cytology*; D002453:Cell Cycle; D019070:Cell Lineage; D002465:Cell Movement; D049109:Cell Proliferation; D004625:Embryo, Nonmammalian / Q000166:cytology; D005854:Germ Cells / Q000166:cytology*; D021621:Imaging, Three-Dimensional; D008954:Models, Biological; D013234:Stem Cells / Q000166:cytology*; D013269:Stochastic Processes
keywords: Germline; Lineage tracing; Proliferation; Stem cells