title: Probing tissue interaction with laser-based cauterization in the early developing Drosophila embryo.
abstract: Tissue morphogenesis is governed by mechanical forces generated by cell cytoskeletal networks. It has been shown that subcellular forces are responsible for cell shape changes. Nevertheless cells in a developing organism do not act in isolation: cells contact and adhere one another, and forces are transmitted from cell-to-cell throughout tissues. Understanding how forces are integrated at the tissue level and finally at the full animal scale is nowadays a major challenge that will allow shedding new light on how embryo morphogenesis takes place. In this chapter, I present a new laser-based technique to probe tissue coupling in a living Drosophila embryo. Such technique allows generating mechanical fix boundaries that can eventually impair or modulate cell flows and tissue displacements to probe tissue interaction.
authors.affiliation: Université Côte d'Azur, CNRS, INSERM, iBV, Nice, France. Electronic address: matteo.rauzi@unice.fr.
authors.forename: M
authors.initials: M
authors.lastname: Rauzi
mesh_terms: D000818:Animals; D048430:Cell Shape / Q000235:genetics; D003599:Cytoskeleton / Q000648:ultrastructure*; D004330:Drosophila / Q000254:growth & development / Q000648:ultrastructure*; D047108:Embryonic Development / Q000235:genetics*; D007834:Lasers; D009024:Morphogenesis / Q000235:genetics*
keywords: Cell flow modulation; Embryo-scale mechanics; Fix boundaries; Laser cauterization; Tissue interaction