title: RNA-Seq Analyses for Two Silkworm Strains Reveals Insight into Their Susceptibility and Resistance to Beauveria bassiana Infection.
abstract: The silkwormBombyx moriis an economically important species. White muscardine caused byBeauveria bassianais the main fungal disease in sericulture, and understanding the silkworm responses toB. bassianainfection is of particular interest. Herein, we investigated the molecular mechanisms underlying these responses in two silkworm strains Haoyue (HY, sensitive toB. bassiana) and Kang 8 (K8, resistant toB. bassiana) using an RNA-seq approach. For each strain, three biological replicates for immersion treatment, two replicates for injection treatment and three untreated controls were collected to generate 16 libraries for sequencing. Differentially expressed genes (DEGs) between treated samples and untreated controls, and between the two silkworm strains, were identified. DEGs and the enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of the two strains exhibited an obvious difference. Several genes encoding cuticle proteins, serine proteinase inhibitors (SPI) and antimicrobial peptides (AMP) and the drug metabolism pathway involved in toxin detoxification were considered to be related to the resistance of K8 toB. bassiana.These results revealed insight into the resistance and susceptibility of two silkworm strains againstB. bassianainfection and provided a roadmap for silkworm molecular breeding to enhance its resistance toB. bassiana.
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China. dongxuxing@126.com.|Sericulture and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China. dongxuxing@126.com.
authors.forename: Dongxu
authors.initials: D
authors.lastname: Xing
authors.affiliation: Sericulture and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China. serilover@126.com.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China. jiangliang@swu.edu.cn.
authors.forename: Liang
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Sericulture and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China. liqr7702@aliyun.com.
authors.forename: Qingrong
authors.initials: Q
authors.lastname: Li
authors.affiliation: Sericulture and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China. xiaoyang1209@163.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Sericulture and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China. yemingq@163.com.
authors.forename: Mingqiang
authors.initials: M
authors.lastname: Ye
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China. xiaqy@swu.edu.cn.
authors.forename: Qingyou
authors.initials: Q
authors.lastname: Xia
mesh_terms: D000820:Animal Diseases / Q000235:genetics* / Q000382:microbiology*; D000818:Animals; D052982:Beauveria; D012831:Bombyx / Q000145:classification / Q000235:genetics* / Q000382:microbiology*; D019295:Computational Biology / Q000379:methods; D060467:Disease Resistance / Q000235:genetics; D020869:Gene Expression Profiling; D020022:Genetic Predisposition to Disease; D059014:High-Throughput Nucleotide Sequencing; D017423:Sequence Analysis, RNA; D013045:Species Specificity; D059467:Transcriptome
keywords: Beauveria bassiana; RNA-seq; resistance; silkworm