title: Electrical Stimulation of Denervated Rat Skeletal Muscle Ameliorates Bone Fragility and Muscle Loss in Early-Stage Disuse Musculoskeletal Atrophy.
abstract: We tested whether daily muscle electrical stimulation (ES) can ameliorate the decrease in cortical bone strength as well as muscle and bone geometric and material properties in the early stages of disuse musculoskeletal atrophy. 7-week-old male F344 rats were randomly divided into three groups: age-matched control group (Cont); a sciatic denervation group (DN); and a DN + direct electrical stimulation group (DN + ES). Denervated tibialis anterior (TA) muscle in the DN + ES group received ES with 16 mA at 10 Hz for 30 min/day, 6 days/week. Micro CT, the three-point bending test, and immunohistochemistry were used to characterize cortical bone mechanical, structural, and material properties of tibiae. TA muscle in the DN + ES group showed significant improvement in muscle mass and myofiber cross-sectional area relative to the DN group. Maximal load and stiffness of tibiae, bone mineral density estimated by micro CT, and immunoreactivity of DMP1 in the cortical bone tissue were also significantly greater in the DN + ES group than in the DN group. These results suggest that daily ES-induced muscle contraction treatment reduced the decrease in muscle mass and cortical bone strength in early-stage disuse musculoskeletal atrophy and is associated with a beneficial effect on material properties such as mineralization of cortical bone tissue.
authors.affiliation: Institute for Human Movement and Medical Sciences, Niigata University of Health and Welfare, 1398 Shimami, Kita-ku, Niigata, 950-3198, Japan. hiroyuki-tamaki@nuhw.ac.jp.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tamaki
authors.affiliation: National Institute of Fitness and Sports in Kanoya, 1 Shiromizu, Kanoya, Kagoshima, 891-2393, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Yotani
authors.affiliation: National Institute of Fitness and Sports in Kanoya, 1 Shiromizu, Kanoya, Kagoshima, 891-2393, Japan.
authors.forename: Futoshi
authors.initials: F
authors.lastname: Ogita
authors.affiliation: Institute for Human Movement and Medical Sciences, Niigata University of Health and Welfare, 1398 Shimami, Kita-ku, Niigata, 950-3198, Japan.
authors.forename: Keishi
authors.initials: K
authors.lastname: Hayao
authors.affiliation: Institute for Human Movement and Medical Sciences, Niigata University of Health and Welfare, 1398 Shimami, Kita-ku, Niigata, 950-3198, Japan.
authors.forename: Kouki
authors.initials: K
authors.lastname: Nakagawa
authors.affiliation: Tohoku Fukushi University, 1‑8‑1 Kunimi, Aoba‑ku, Sendai, Miyagi, 981‑8522, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Sugawara
authors.affiliation: Institute for Human Movement and Medical Sciences, Niigata University of Health and Welfare, 1398 Shimami, Kita-ku, Niigata, 950-3198, Japan.
authors.forename: Hikari
authors.initials: H
authors.lastname: Kirimoto
authors.affiliation: Institute for Human Movement and Medical Sciences, Niigata University of Health and Welfare, 1398 Shimami, Kita-ku, Niigata, 950-3198, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Onishi
authors.affiliation: Aichi University of Education, 1 Hirosawa, Igaya, Kariya, Aichi, 448-8542, Japan.
authors.forename: Norikatsu
authors.initials: N
authors.lastname: Kasuga
authors.affiliation: Institute for Human Movement and Medical Sciences, Niigata University of Health and Welfare, 1398 Shimami, Kita-ku, Niigata, 950-3198, Japan.|Niigata Rehabilitation Hospital, 761 Kisaki, Kita-ku, Niigata, 950-3304, Japan.
authors.forename: Noriaki
authors.initials: N
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D001284:Atrophy; D001842:Bone and Bones / Q000473:pathology; D004558:Electric Stimulation; D050723:Fractures, Bone / Q000628:therapy*; D008297:Male; D009121:Muscle Denervation / Q000379:methods; D018485:Muscle Fibers, Skeletal; D018482:Muscle, Skeletal / Q000502:physiology*; D011916:Rats, Inbred F344; D055948:Sarcopenia / Q000628:therapy*; D013977:Tibia / Q000503:physiopathology; D055114:X-Ray Microtomography / Q000379:methods
keywords: Bone strength; Denervation; Dentin matrix protein; Electrical stimulation; Muscle contraction