title: Genistein inhibits hypoxia, ischemic-induced death, and apoptosis in PC12 cells.
abstract: A hypoxia/ischemia neuronal model was established in PC12 cells using oxygen-glucose deprivation (OGD). OGD-induced neuronal death, apoptosis, glutamate receptor subunit GluR2 expression, and potassium channel currents were evaluated in the present study to determine the effects of genistein in mediating the neuronal death and apoptosis induced by hypoxia and ischemia, as well as its underlying mechanism. OGD exposure reduced the cell viability, increased apoptosis, decreased the GluR2 expression, and decreased the voltage-activated potassium currents. Genistein partially reversed the effects induced by OGD. Therefore, genistein may prevent hypoxia/ischemic-induced neuronal apoptosis that is mediated by alterations in GluR2 expression and voltage-activated potassium currents.
authors.affiliation: College of Life Sciences, Nankai University, Tianjin 300071, China.
authors.forename: Yu-Xiang
authors.initials: YX
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Nankai University, Tianjin 300071, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Tian
authors.affiliation: College of Life Sciences, Nankai University, Tianjin 300071, China.
authors.forename: Cong-Cong
authors.initials: CC
authors.lastname: He
authors.affiliation: College of Life Sciences, Nankai University, Tianjin 300071, China.
authors.forename: Xue-Ling
authors.initials: XL
authors.lastname: Ma
authors.affiliation: College of Life Sciences, Nankai University, Tianjin 300071, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Nankai University, Tianjin 300071, China.
authors.forename: Hong-Gang
authors.initials: HG
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Nankai University, Tianjin 300071, China.
authors.forename: Di
authors.initials: D
authors.lastname: An
authors.affiliation: College of Life Sciences, Nankai University, Tianjin 300071, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Heng
authors.affiliation: Department of Nutrition, Tianjin Institute of Health and Environmental Medicine, Tianjin 300050, China.
authors.forename: Yu-Gang
authors.initials: YG
authors.lastname: Jiang
authors.affiliation: College of Life Sciences, Nankai University, Tianjin 300071, China. Electronic address: liuyanq@nankai.edu.cn.
authors.forename: Yan-Qiang
authors.initials: YQ
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D015687:Cell Hypoxia / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D019833:Genistein / Q000494:pharmacology*; D007511:Ischemia / Q000188:drug therapy; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016716:PC12 Cells; D015221:Potassium Channels / Q000187:drug effects; D051381:Rats; D018091:Receptors, AMPA / Q000378:metabolism*
chemical_list: D018696:Neuroprotective Agents; D015221:Potassium Channels; D018091:Receptors, AMPA; D019833:Genistein; C104722:glutamate receptor ionotropic, AMPA 2
keywords: Apoptosis; Genistein; Hypoxia/ischemia; Neuroprotection; PC12 cells