title: Isolation of four phenolic compounds from Mangifera indica L. flowers by using normal phase combined with elution extrusion two-step high speed countercurrent chromatography.
abstract: Two-step high speed countercurrent chromatography method, following normal phase and elution-extrusion mode of operation by using selected solvent systems, was introduced for phenolic compounds separation. Phenolic compounds including gallic acid, ethyl gallate, ethyl digallate and ellagic acid were separated from the ethanol extract of mango (Mangifera indica L.) flowers for the first time. In the first step, gallic acid of 3.7mg and ethyl gallate of 3.9mg with the purities of 98.87% and 99.55%, respectively, were isolated by using hexane-ethylacetate-methanol-water (4:6:4:6, v/v) in normal phase high speed countercurrent chromatography from 200mg of crude extract, while ethyl digallate and ellagic acid were collected in the form of mixture fraction. In the second step, further purification of the mixture was carried out with the help of another selected solvent system of dichloromethane-methanol-water (4:3:2, v/v) following elusion-extrusion mode of operation. Ethyl digallate of 3.8mg and ellagic acid of 5.7mg were separated well with high purities of 98.68% and 99.71%, respectively. The separated phenolic compounds were identified and confirmed by HPLC, UPLC-QTOF/ESI-MS,1H and13C NMR spectrometric analysis.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 3rd North East Road, Chaoyang District, Beijing 100029, China.
authors.forename: Nusrat
authors.initials: N
authors.lastname: Shaheen
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 3rd North East Road, Chaoyang District, Beijing 100029, China.
authors.forename: Yanzhen
authors.initials: Y
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 3rd North East Road, Chaoyang District, Beijing 100029, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Geng
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 3rd North East Road, Chaoyang District, Beijing 100029, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Shao
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 3rd North East Road, Chaoyang District, Beijing 100029, China. Electronic address: weiyun@mail.buct.edu.cn.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wei
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D035264:Flowers / Q000737:chemistry; D031022:Mangifera / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010636:Phenols / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D010636:Phenols; D010936:Plant Extracts
keywords: (1)HNMR; (13)C NMR; HSCCC; Mangifera indica L. flowers; Phenolic compounds; UPLC-QTOF/ESI–MS