title: Protective impact of resveratrol in experimental rat model of hyperoxaluria.
abstract: OBJECTIVE
  Resveratrol (RES) is a polyphenol with antioxidant, anti-inflammatory, and many other physiological effects on tissues. In the present study, the effect of resveratrol in hyperoxaluria driven nephrolithiasis/nephrocalcinosis is investigated.
  
  
  METHODS
  Wistar-Albino rats of 250-300 g (male, n = 24) were included in the present study. The rats were randomized into three groups: Group 1 consisted of the controls (n = 8), Group 2 of hyperoxaluria (1% ethylene glycol (EG), n = 8), and Group 3 of the treatment (1% EG + 10 mg/kg of RES, n = 8) group. At the beginning and fifth week of the study, two rats from each group were placed in metabolic cages for 24 h and their urine was collected. At the end of the study, the rats were killed and their blood was collected from the vena cava inferior. The right kidneys of the rats were used for biochemical and the left ones for immunohistochemical analyzes. Malondialdehyde (MDA), catalase, urea, calcium, oxalate, and creatinine clearance were studied in the blood, urine, and kidney tissues. Moreover, routine histological evaluation, and p38-MAPK and NFkB immunohistochemical analyses were conducted.
  
  
  RESULTS
  In the hyperoxaluria group, urinary oxalate levels were higher than the control group; yet, lower in the treatment group compared to hyperoxaluria group (p < 0.05). Serum MDA levels in the hyperoxaluria group were higher than the control group; but in the treatment group it is lower than the hyperoxaluria group (p < 0.05). P38 MAPK activity was higher in the hyperoxaluria group compared to the control (p < 0.05). However, in terms of p38 MAPK activity, there were no statistically significant difference between hyperoxaluria and the treatment group (p < 0.069). Whereas NFkB activity in the hyperoxaluria group is higher than the control (p < 0.001), no statistically significant difference was observed with the treatment group.
  
  
  CONCLUSIONS
  In the present study, resveratrol was seen to prevent hyperoxaluria. With preventing oxidative stress factors and Randall plaque formation caused by free oxygen radicals, resveratrol can be an alternative treatment option that can increase the success rate in preventing stone recurrence in the future.
authors.affiliation: Department of Urology, Medical Faculty, Süleyman Demirel University, Isparta, Turkey.
authors.forename: Taylan
authors.initials: T
authors.lastname: Oksay
authors.affiliation: Department of Urology, Afyonkarahisar State Hospital, Afyonkarahisar, Turkey.
authors.forename: Sedat
authors.initials: S
authors.lastname: Yunusoğlu
authors.affiliation: Department of Biochemistry, Medical Faculty, Suleyman Demirel University, Isparta, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Calapoğlu
authors.affiliation: Department of Histology and Embryology, Medical Faculty, Süleyman Demirel University, Isparta, Turkey.
authors.forename: I
authors.initials: I
authors.lastname: Aydın Candan
authors.affiliation: Department of Medical Biology, Medical Faculty, Süleyman Demirel Uviversity, Isparta, Turkey.
authors.forename: İbrahim
authors.initials: İ
authors.lastname: Onaran
authors.affiliation: Department of Urology, Urology Policlinic, Konya Training and Research Hospital, PK 42090, Hacı Şaban Mah. Meram Yeniyol Caddesi No: 97, Meram, Konya, Turkey. osmanergun77@mynet.com.
authors.forename: Osman
authors.initials: O
authors.lastname: Ergün
authors.affiliation: Department of Urology, Medical Faculty, Süleyman Demirel University, Isparta, Turkey.
authors.forename: Alper
authors.initials: A
authors.lastname: Özorak
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001707:Biopsy, Needle; D004195:Disease Models, Animal; D019855:Ethylene Glycol / Q000494:pharmacology; D006959:Hyperoxaluria / Q000473:pathology / Q000517:prevention & control*; D007150:Immunohistochemistry; D007669:Kidney Calculi / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D000077185:Resveratrol; D018709:Statistics, Nonparametric; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D013267:Stilbenes; D019855:Ethylene Glycol; D000077185:Resveratrol
keywords: Calcium oxalate; Ethylene glycol; Hyperoxaluria; Rat; Resveratrol