title: BCL6 mRNA Expression Level in Invasive Duct Carcinoma not otherwise Specified.
abstract: BACKGROUND
  B-Cell Lymphoma 6 (BCL6) has an oncogenic role in tumourigenesis of various malignancies. It represses genes involved in terminal differentiation and plays complementary role with Signal Transducer and Activator of Transcription 3 (STAT3) in triple-negative breast cancer cellular function.
  
  
  OBJECTIVE
  To evaluate the expression of BCL6 in cancer breast and determine its correlation with the clinico-pathological features including the molecular subtype of breast carcinoma.
  
  
  METHODS
  This prospective case control study was carried out on 150 patients, divided into 100 cases of invasive duct carcinoma not otherwise specified and 50 benign breast lesions including fibroadenoma and fibrocystic disease. Fresh tissues were excised, which were then subjected to RNA extraction. The BCL6 mRNA level was assessed using real-time reverse transcription Polymerase Chain Reaction (PCR).
  
  
  RESULTS
  There was a significant higher levels of BCL6 mRNA in malignant cases compared to benign ones (p<0.001). The level of BCL6 mRNA was higher in cases showing advanced tumor stage (p<0.04), triple negative subtype and associated in situ component (p<0.001) compared to cases with an early stage, luminal or Her 2-neu positive subtypes and those lacking in situ component.
  
  
  CONCLUSIONS
  BCL6 is up-regulated in breast cancer and is associated with poor prognostic features such as advanced stage and triple negative molecular subtype. BCL6 inhibitors might be considered as targeted therapy for breast cancer.
authors.affiliation: Assistant Professor, Department of Biochemistry, Shebein Elkom , Menoufia, Egypt .
authors.forename: Eman
authors.initials: E
authors.lastname: Badr
authors.affiliation: Lecturer, Department of Biochemistry, Shebein Elkom , Menoufia, Egypt .
authors.forename: Eman
authors.initials: E
authors.lastname: Masoud
authors.affiliation: Professor, Department of Pathology, Shebein Elkom , Menoufia, Egypt .
authors.forename: Asmaa Gaber
authors.initials: AG
authors.lastname: Abdou
authors.affiliation: Lecturer, Department of Pathology, Shebein Elkom , Menoufia, Egypt .
authors.forename: Marwa Serag
authors.initials: MS
authors.lastname: Eldien
keywords: Breast cancer; Polymerase chain reaction; Stage; Target therapy; Triple negative