title: A Novel Application of Non-Destructive Readout Technology to Localisation Microscopy.
abstract: The fitting precision in localisation microscopy is highly dependent on the signal to noise ratio. To increase the quality of the image it is therefore important to increase the signal to noise ratio of the measurements. We present an imaging system for localisation microscopy based on non-destructive readout camera technology that can increase the signal to noise ratio of localisation based microscopy. This approach allows for much higher frame rates through subsampling a traditional camera frame. By matching the effective exposure to both the start time and duration of a single molecule we diminish the effects of read noise and temporal noise. We demonstrate the application of this novel method to localisation microscopy and show both an increase in the attainable signal to noise ratio of data collection and an increase in the number of detected events.
authors.affiliation: The Department of Molecular Biology and Biotechnology, Firth Court, Western Bank, Sheffield, S10 2TN, United Kingdom.
authors.forename: Samuel F H
authors.initials: SF
authors.lastname: Barnett
authors.affiliation: The Department of Physics and Astronomy, Hicks Building, Hounsfield Road, Sheffield, S3 7RH, United Kingdom.
authors.forename: Mary
authors.initials: M
authors.lastname: Snape
authors.affiliation: The Department of Molecular Biology and Biotechnology, Firth Court, Western Bank, Sheffield, S10 2TN, United Kingdom.
authors.forename: C Neil
authors.initials: CN
authors.lastname: Hunter
authors.affiliation: School of Mathematics and Statistics, Hicks Building, Hounsfield Road, Sheffield, S3 7RH, United Kingdom.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Juárez
authors.affiliation: The Department of Physics and Astronomy, Hicks Building, Hounsfield Road, Sheffield, S3 7RH, United Kingdom.
authors.forename: Ashley J
authors.initials: AJ
authors.lastname: Cadby