title: Comparison of analytic performances of Cellsearch and iFISH approach in detecting circulating tumor cells.
abstract: Circulating tumor cells (CTCs) have been widely used to predict the prognosis of breast cancer patients. The aim of the present study was to compare the performances of Cellsearch and immunostaining-fluorescence in situ hybridization (iFISH) in detecting CTCs in breast cancer patients. Forty-five newly diagnosed breast cancer patients and 14 healthy donors were recruited and their CTCs were detected by both Cellsearch and iFISH. Correlation between clinicopathological features and CTCs was investigated. We found that the positive rate of CTC detected by iFISH was significantly higher than by Cellsearch system (91% vs 38%). The CTC count, detected either by iFISH or Cellsearch, was not significantly associated with clinical pictures of patients with breast cancer. Therefore, we concluded that, compared to conventional Cellsearch CTC detection, in situ karyotypic identification performed by iFISH had higher detection rate. Therefore, iFISH may be more clinically useful than Cellsearch system.
authors.affiliation: Department of Thyroid and Breast Surgery, Changhai Hospital, The Second Military Medical University, Shanghai, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Sheng
authors.affiliation: Department of Vascular and Endocrine Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Thyroid and Breast Surgery, Changhai Hospital, The Second Military Medical University, Shanghai, China.
authors.forename: Hengyu
authors.initials: H
authors.lastname: Li
authors.affiliation: Biotecan Medical Diagnostics Co., Ltd, Zhangjiang Center for Translational Medicine, Shanghai, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Vascular and Endocrine Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Jianghao
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Vascular and Endocrine Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Chenyang
authors.initials: C
authors.lastname: He
authors.affiliation: Department of Vascular and Endocrine Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Yongping
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Vascular and Endocrine Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Lv
authors.affiliation: Department of Vascular and Endocrine Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Juliang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Biotecan Medical Diagnostics Co., Ltd, Zhangjiang Center for Translational Medicine, Shanghai,China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Nephrology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of Vascular and Endocrine Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D001943:Breast Neoplasms / Q000097:blood* / Q000235:genetics / Q000473:pathology; D002877:Chromosomes, Human; D000071858:Epithelial Cell Adhesion Molecule / Q000097:blood; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D059785:Karyotype; D007621:Karyotyping / Q000379:methods*; D007633:Keratins / Q000097:blood; D008875:Middle Aged; D009360:Neoplastic Cells, Circulating / Q000737:chemistry* / Q000473:pathology; D011237:Predictive Value of Tests; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000097:blood; D015203:Reproducibility of Results; D014746:Vimentin / Q000097:blood; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D000071858:Epithelial Cell Adhesion Molecule; D007633:Keratins; D018719:Erb-b2 Receptor Tyrosine Kinases; D014746:Vimentin; C499379:EPCAM protein, human; C508053:ERBB2 protein, human
keywords: aneuploidy; breast cancer; cellsearch; circulating tumor cells (CTC); subtraction enrichment