title: RNA-Seq-based transcriptomic and metabolomic analysis reveal stress responses and programmed cell death induced by acetic acid in Saccharomyces cerevisiae.
abstract: As a typical harmful inhibitor in cellulosic hydrolyzates, acetic acid not only hinders bioethanol production, but also induces cell death in Saccharomyces cerevisiae. Herein, we conducted both transcriptomic and metabolomic analyses to investigate the global responses under acetic acid stress at different stages. There were 295 up-regulated and 427 down-regulated genes identified at more than two time points during acetic acid treatment (150 mM, pH 3.0). These differentially expressed genes (DEGs) were mainly involved in intracellular homeostasis, central metabolic pathway, transcription regulation, protein folding and stabilization, ubiquitin-dependent protein catabolic process, vesicle-mediated transport, protein synthesis, MAPK signaling pathways, cell cycle, programmed cell death, etc. The interaction network of all identified DEGs was constructed to speculate the potential regulatory genes and dominant pathways in response to acetic acid. The transcriptional changes were confirmed by metabolic profiles and phenotypic analysis. Acetic acid resulted in severe acidification in both cytosol and mitochondria, which was different from the effect of extracellular pH. Additionally, the imbalance of intracellular acetylation was shown to aggravate cell death under this stress. Overall, this work provides a novel and comprehensive understanding of stress responses and programmed cell death induced by acetic acid in yeast.
authors.affiliation: Department of Food Science and Nutrition, Key Laboratory for Food Microbial Technology of Zhejiang Province, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
authors.forename: Yachen
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Department of Food Science and Nutrition, Key Laboratory for Food Microbial Technology of Zhejiang Province, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
authors.forename: Jingjin
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Food Science and Nutrition, Key Laboratory for Food Microbial Technology of Zhejiang Province, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Fan
authors.affiliation: Department of Food Science and Nutrition, Key Laboratory for Food Microbial Technology of Zhejiang Province, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
authors.forename: Qihe
authors.initials: Q
authors.lastname: Chen
mesh_terms: D019342:Acetic Acid / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D059014:High-Throughput Nucleotide Sequencing; D006863:Hydrogen-Ion Concentration; D053858:Metabolic Networks and Pathways / Q000187:drug effects* / Q000235:genetics; D055442:Metabolome; D008928:Mitochondria / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological / Q000187:drug effects* / Q000235:genetics; D059467:Transcriptome
chemical_list: D019342:Acetic Acid