title: B7-H4 deficiency in salivary gland of patients with primary Sjögren's syndrome impairs the regulatory effect on T cells.
abstract: OBJECTIVE
  Our previous study confirmed the defect of B7-H4 expression in peripheral blood and salivary glands of patients with primary Sjögren's syndrome (pSS). The aim of this study was to analyze the effect of the deficit expression of B7-H4 on CD4+T cells.
  
  
  METHODS
  CD4+T cells were purified by magnetic-activated cell sorting MACS. The proliferation and cytokine production of CD4+T cells co-cultured with purified salivary gland epithelial cells (SGECs) from pSS or non-SS sicca syndrome were detected.
  
  
  RESULTS
  By co-culturing the gland cells with CD4+T cells, we found the proliferation of CD4+T cells was significantly suppressed. The effect was weaker when SGECs from pSS patients were used compared to that from non-pSS sicca syndrome controls. Simultaneously, the productions of cytokines interleukin (IL)-5, IL-13, IL-17A, IL-6 in supernatant were reduced and also SGECs from pSS patients decreased them less than that from non-SS controls.
  
  
  CONCLUSIONS
  The decrease of B7-H4 expression in salivary glands of SS patients contributes to the defect of negatively regulating the inflammation caused by CD4+T cells, thereby providing new insights into the role of B7-H4 in the inflammatory process of salivary glands in SS.
authors.affiliation: Department of Rheumatology and Immunology, Affiliated Anhui Provincial Hospital, Anhui Medical University, Hefei, Anhui.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Rheumatology and Immunology, Affiliated Anhui Provincial Hospital, Anhui Medical University, Hefei, Anhui.|Department of Nephrology, Renmin Hospital of Three Gorges University, Yichang, China.
authors.forename: Daliang
authors.initials: D
authors.lastname: Yu
authors.affiliation: Department of Rheumatology and Immunology, Affiliated Anhui Provincial Hospital, Anhui Medical University, Hefei, Anhui.
authors.forename: Ning
authors.initials: N
authors.lastname: Yu
authors.affiliation: Department of Rheumatology and Immunology, Affiliated Anhui Provincial Hospital, Anhui Medical University, Hefei, Anhui.
authors.forename: Ximei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Rheumatology and Immunology, Affiliated Anhui Provincial Hospital, Anhui Medical University, Hefei, Anhui.
authors.forename: Xiangpei
authors.initials: X
authors.lastname: Li
authors.affiliation: Centre for Transplantation and Renal Research, Westmead Hospital, University of Sydney, Sydney, New South Wales, Australia.
authors.forename: David C H
authors.initials: DCH
authors.lastname: Harris
authors.affiliation: Centre for Transplantation and Renal Research, Westmead Hospital, University of Sydney, Sydney, New South Wales, Australia.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D002450:Cell Communication; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines / Q000378:metabolism; D004847:Epithelial Cells / Q000276:immunology / Q000378:metabolism*; D005260:Female; D006801:Humans; D018189:Immunomagnetic Separation; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D012469:Salivary Glands / Q000276:immunology / Q000378:metabolism*; D012859:Sjogren's Syndrome / Q000175:diagnosis / Q000276:immunology / Q000378:metabolism*; D060907:V-Set Domain-Containing T-Cell Activation Inhibitor 1 / Q000172:deficiency* / Q000276:immunology
chemical_list: D016207:Cytokines; D060907:V-Set Domain-Containing T-Cell Activation Inhibitor 1; C475826:VTCN1 protein, human
keywords: B7-H4; Sjögren's syndrome; co-stimulatory molecule; salivary gland