title: Rapid High Performance Liquid Chromatography Determination and Optimization of Extraction Parameters of the α-Asarone Isolated from Perilla frutescens L.
abstract: Response surface methodology (RSM), based on a central composite design, was used to determine the best liquid-to-raw material ratio (10:3-15 mL/g), extraction time (1-3 h), and ethanol concentration (50%-100%) for maximum content of α-asarone from Perilla frutescens (PF) extract. Experimental values of α-asarone were 9.51-46.36 mg/g; the results fitted a second-order quadratic polynomial model and correlated with the proposed model (R2 > 0.9354). The best conditions were obtained with extraction time of 1.76 h, liquid-to-raw material ratio of 10:13.5 mL/g, and ethanol concentration of 90.37%. Under these conditions, the model predicted extraction content of 40.56 mg/g, while experimental PF content of α-asarone was 43.84 mg/g dried plant. Optimized conditions determined for maximum content of α-asarone were similar to the experimental range. Experimental values agreed with those predicted, thus validating and indicating suitability of both the model and the RSM approach for optimizing extraction conditions. In addition, a reliable, reproducible and accurate method for the quantitative determination of α-asarone by High Performance Liquid Chromatography (HPLC) analysis was developed with limit of detection (LOD), limit of quantitation (LOQ) values of 0.10 and 0.29 µg/mL and excellent linearity (R2 > 0.9999).
authors.affiliation: Department of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Korea. isohsh@gmail.com.
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Hwang
authors.affiliation: Institute of Natural Medicine, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Korea. ksh8976@nate.com.
authors.forename: Shin Hwa
authors.initials: SH
authors.lastname: Kwon
authors.affiliation: Department of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Korea. yhkang@hallym.ac.kr.
authors.forename: Young-Hee
authors.initials: YH
authors.lastname: Kang
authors.affiliation: Institute of Natural Medicine, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Korea. jyolee@hallym.ac.kr.|Department of Biochemistry, School of Medicine, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Korea. jyolee@hallym.ac.kr.
authors.forename: Jae-Yong
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Department of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Korea. limss@hallym.ac.kr.|Institute of Natural Medicine, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Korea. limss@hallym.ac.kr.|Institute of Korean Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Korea. limss@hallym.ac.kr.
authors.forename: Soon Sung
authors.initials: SS
authors.lastname: Lim
mesh_terms: D000081248:Allylbenzene Derivatives; D000840:Anisoles / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000737:chemistry; D057230:Limit of Detection; D031344:Perilla frutescens / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry
chemical_list: D000081248:Allylbenzene Derivatives; D000840:Anisoles; D010936:Plant Extracts; D012997:Solvents; C012195:asarone; D000431:Ethanol
keywords: HPLC; Perilla frutescens L.; optimization; response surface methodology; validation; α-asarone