title: Heat shock protein 70 protects cardiomyocytes through suppressing SUMOylation and nucleus translocation of phosphorylated eukaryotic elongation factor 2 during myocardial ischemia and reperfusion.
abstract: Myocardial ischemia and reperfusion (MIR) results in cardiomyocyte apoptosis with severe outcomes, which blocks cardiac tissue recovering from myocardial ischemia diseases. Heat shock protein 70 (HSP70) is one of protective molecule chaperones which could regulate the nucleus translocation of other proteins. In addition, eukaryotic elongation factor 2 (eEF2), which modulates protein translation process, is vital to the recovery of heart during MIR. However, the relationship between HSP70 and eEF2 and its effects on MIR are unclear. The expression and relationship between HSP70 and eEF2 is confirmed by western blot, immunoprecipitation in vitro using cardiomyocyte cell line H9c2 and in vivo rat MIR model. The further investigation was conducted in H9c2 cells with detection for cell-cycle and apoptosis. It is revealed that eEF2 interacted and be regulated by HSP70, which kept eEF2 as dephosphorylated status and preserved the function of eEF2 during MIR. In addition, HSP70 suppressed the nucleus translocation of phosphorylated eEF2, which inhibited cardiomyocyte apoptosis during myocardial reperfusion stage. Furthermore, HSP70 also interacted with C-terminal fragment of eEF2, which could reverse the nucleus translocation and cardiomyocyte apoptosis caused by N-terminal fragment of eEF2. HSP70 draw on advantage and avoid defect of MIR through regulating phosphorylation and nucleus translocation of eEF2.
authors.affiliation: Department of Cardiology, Affiliated Hospital of Nantong University, Nantong, 226001, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Pathogen Biology, Medical College, Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Laboratory Animals Centre, Nantong University, 19 Qixiu Road, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Jin
authors.initials: J
authors.lastname: Miao
authors.affiliation: Laboratory Animals Centre, Nantong University, 19 Qixiu Road, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Shengcun
authors.initials: S
authors.lastname: Wang
authors.affiliation: Laboratory Animals Centre, Nantong University, 19 Qixiu Road, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Liucheng
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Thoracic Surgery, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Daliang
authors.initials: D
authors.lastname: Yan
authors.affiliation: Department of Cardiology, Affiliated Hospital of Nantong University, Nantong, 226001, People's Republic of China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Cardiology, Affiliated Hospital of Nantong University, Nantong, 226001, People's Republic of China.
authors.forename: Wanwan
authors.initials: W
authors.lastname: Guo
authors.affiliation: Department of Cardiology, Affiliated Hospital of Nantong University, Nantong, 226001, People's Republic of China. ntwx0513@163.com.
authors.forename: Xiang
authors.initials: X
authors.lastname: Wu
authors.affiliation: Coinnovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, People's Republic of China. shag@ntu.edu.cn.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, 226001, Jiangsu, People's Republic of China. shag@ntu.edu.cn.
authors.forename: Aiguo
authors.initials: A
authors.lastname: Shen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D001692:Biological Transport / Q000235:genetics; D002460:Cell Line; D002467:Cell Nucleus / Q000235:genetics; D054736:Elongation Factor 2 Kinase / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D015428:Myocardial Reperfusion Injury / Q000235:genetics* / Q000473:pathology; D032383:Myocytes, Cardiac / Q000473:pathology; D010766:Phosphorylation; D051381:Rats; D058207:Sumoylation
chemical_list: D018840:HSP70 Heat-Shock Proteins; C517691:Eef2k protein, rat; D054736:Elongation Factor 2 Kinase
keywords: Cardiomyocyte apoptosis; Eukaryotic elongation factor 2; Heat shock protein 70; Myocardial ischemia and reperfusion; Nucleus translocation; Phosphorylation