title: Evaluation of superficially porous and fully porous columns for analysis of drugs in plasma samples by UHPLC-MS/MS.
abstract: This work compares the performance of recently introduced C18superficially porous columns (four columns) and C18fully porous columns (two columns), with different particle sizes, during the analysis of drugs in plasma samples by MS/MS detection. The following chromatographic parameters were evaluated for all the columns: reduced plate height (h) vs reduced linear velocity (v), impedance vs v, chromatographic run time vs flow rate (mLmin-1), backpressure vs flow rate (mLmin-1), resolution, peak capacity, asymmetry, and retention factor. By using DAD detection, hydrophobicity, silanol activity, and metal impurities were also assessed for the columns. The columns with charged surface displayed improved chromatographic efficiency for the drugs in their ionized form. The columns with particles smaller than 2μm (Cortecs 1.6μm, Acquity 1.7μm, and Kinetex 1.7μm) presented higher chromatographic efficiency for the drugs, which were in their partially ionized form. The generated mathematical models were able to predict the backpressure and chromatographic run time at different flow rates for all the columns. Considering the efficiency, impedance, resolution, peak capacity, retention factor, and hydrophobicity, Cortecs 1.6μm and Acquity 1.7μm were the columns that given the best performance during analysis of the target drugs in plasma samples.
authors.affiliation: Departamento de Química, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Vinicius Ricardo
authors.initials: VR
authors.lastname: Acquaro
authors.affiliation: Instituto de Química de São Carlos, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Fernando Mauro
authors.initials: FM
authors.lastname: Lanças
authors.affiliation: Departamento de Química, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil. Electronic address: mariaeqn@ffclrp.usp.br.
authors.forename: Maria Eugênia Costa
authors.initials: ME
authors.lastname: Queiroz
mesh_terms: D000465:Algorithms; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D004364:Pharmaceutical Preparations / Q000097:blood*; D016062:Porosity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004364:Pharmaceutical Preparations
keywords: Chromatographic parameters; Drugs; Plasma sample; Superficially porous columns; UHPLC–MS/MS