title: Captopril apparently increase and cisplatin apparently decrease human albumin concentration in artificial urinary solutions.
abstract: BACKGROUND
  Measurement of urinary albumin (HSA) is very important in diagnostic of kidney diseases. Much less is known about the possible impact of substances present in urine together with albumin on the results of measurements.
  
  
  METHODS
  We investigated the effect of the presence of captopril and cisplatin in the solution on the result of the determination of HSA by native polyacrylamide gel electrophoresis. Protein conformation in the absence and presence of the drugs was examined using Fourier Transform Infrared Spectroscopy (FTIR).
  
  
  RESULTS
  The presence of captopril apparently increases HSA concentration while cisplatin causes an apparent decrease in the HSA concentration. The presence of both drugs also influence the secondary structure forms of HSA albumin investigated by FTIR.
  
  
  CONCLUSIONS
  Both drugs tested in the concentration of human use can have an impact on the results of determination of albumin in urine which can influence clinical decision.
authors.affiliation: Institute of Environmental Engineering, The John Paul II Catholic University of Lublin, Lublin, Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Kuzioła
authors.affiliation: Institute of Environmental Engineering, The John Paul II Catholic University of Lublin, Lublin, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Marczewska
authors.affiliation: Pope John Paul II Regional Hospital in Zamość and Faculty of Medical Science Lublin University of Economy and Innovations, Lublin, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Marczewski
mesh_terms: D002216:Captopril / Q000652:urine*; D002945:Cisplatin / Q000652:urine*; D006801:Humans; D008954:Models, Biological; D063347:Native Polyacrylamide Gel Electrophoresis / Q000592:standards*; D000075462:Serum Albumin, Human / Q000652:urine*; D016482:Urinalysis / Q000592:standards*
chemical_list: D002216:Captopril; D002945:Cisplatin; D000075462:Serum Albumin, Human
keywords: drugs interference; native polyacrylamide gel electrophoresis; urine albumin determination