title: Biodegradation of the benzo[a]pyrene-contaminated sediment of the Jiaozhou Bay wetland using Pseudomonas sp. immobilization.
abstract: To remove benzo[a]pyrene (BaP) that has accumulated in the Jiaozhou Bay wetland sediment, two strains (JB1 and JB2) were selected from the BaP-contaminated the wetland sediment and immobilized in coal cinder and chitosan beads using entrapping and surface adsorption methods. Biodegradation of BaP in sediment was carried out in pots. The results showed that, supported by the coal cinder and chitosan beads, 71.9, 65.5, 58.9 and 66.1% of the BaP in the immobilized cells was degraded after 40d. These percentages were clearly higher than the 47.7% that degraded from free cells. Kinetic analysis indicated that the immobilized gel-beads might remove BaP by multiple control steps. Compared to the chitosan, coal cinder-entrapping beads exhibited a higher removal rate for BaP; however, the degradation rates from coal cinder- and chitosan-surface adsorption beads were almost the same. This result indicates that in addition to the BaP-degrading bacteria, carrier materials and immobilizing methods play an important role in determining the success of a biodegradation strategy.
authors.affiliation: College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Jin
authors.affiliation: College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China; Key Laboratory of Marine Environment and Ecology, Ministry of Education, Qingdao 266100, China. Electronic address: weijunas@ouc.edu.cn.
authors.forename: Weijun
authors.initials: W
authors.lastname: Tian
authors.affiliation: College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
authors.forename: Kaili
authors.initials: K
authors.lastname: Qiao
authors.affiliation: College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Gong
mesh_terms: D061006:Bays; D001564:Benzo(a)pyrene / Q000378:metabolism*; D001673:Biodegradation, Environmental; D018914:Cells, Immobilized / Q000378:metabolism*; D002681:China; D019015:Geologic Sediments; D007700:Kinetics; D011549:Pseudomonas / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D053833:Wetlands
chemical_list: D014874:Water Pollutants, Chemical; D001564:Benzo(a)pyrene
keywords: Benzo[a]pyrene; Chitosan; Coal cinder; Immobilization; Jiaozhou Bay; Pseudomonas sp.