title: Isolation, Characterization and Transcriptome Analysis of a Cytokinin Receptor MutantOsckt1in Rice.
abstract: Cytokinins play important roles in regulating plant development, including shoot and root meristems, leaf longevity, and grain yield. However, thein plantafunctions of rice cytokinin receptors have not been genetically characterized yet. Here we isolated a rice mutant,Osckt1, with enhanced tolerance to cytokinin treatment. Further analysis showed thatOsckt1was insensitive to aromatic cytokinins but responded normally to isoprenoid and phenylurea-type cytokinins. Map-based cloning revealed that the mutation occurred in a putative cytokinin receptor gene, histidine kinase 6 (OsHK6).OsCKT1was found to be expressed in various tissues throughout the plant and the protein was located in the endoplasmic reticulum. In addition, whole-genome gene expression profiling analysis showed thatOsCKT1was involved in cytokinin regulation of a number of biological processes, including secondary metabolism, sucrose and starch metabolism, chlorophyll synthesis, and photosynthesis. Our results demonstrate thatOsCKT1plays important roles in cytokinin perception and control of root development in rice.
authors.affiliation: College of Science and Technology, Ningbo University Ningbo, China.
authors.forename: Wona
authors.initials: W
authors.lastname: Ding
authors.affiliation: School of Marine Sciences, Ningbo University Ningbo, China.
authors.forename: Huishan
authors.initials: H
authors.lastname: Tong
authors.affiliation: College of Science and Technology, Ningbo University Ningbo, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Zheng
authors.affiliation: College of Science and Technology, Ningbo University Ningbo, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ye
authors.affiliation: College of Science and Technology, Ningbo University Ningbo, China.
authors.forename: Zhichong
authors.initials: Z
authors.lastname: Pan
authors.affiliation: Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences Ningbo, China.
authors.forename: Botao
authors.initials: B
authors.lastname: Zhang
authors.affiliation: College of Science and Technology, Ningbo University Ningbo, China.
authors.forename: Shihua
authors.initials: S
authors.lastname: Zhu
keywords: Oryza sativa; cytokinin; histidine kinase; rice; transcriptome