title: Polymorphisms of toll-like receptors 2 and 9 and severity and prognosis of bacterial meningitis in Chinese children.
abstract: Toll-like receptors (TLRs) play a crucial role in innate immunity, protecting the host from bacterial pathogens. We investigated whether bacterial meningitis (BM) in children was associated with gene polymorphisms in TLR2 (rs3804099), TLR3 (rs3775291 and rs3775290) and TLR9 (rs352139 and rs352140). Blood samples were taken from 218 child patients with confirmed BM and 330 healthy adult controls (HC) and polymorphisms of these genes were analyzed by PCR-based sequencing. For TLR2 rs3804099, frequencies of the minor allele C were markedly higher in patients with severe BM (defined as CSF glucose concentration ≤ 1.5 mmol/L and seizures) than those without (43.5% and 40.1% vs. 30.1% and 29.1%, p = 0.008 and p = 0.016, respectively). For TLR9 rs352139, patients who carried genotype AA and minor allele A developed seizures less often than those without (OR = 0.289, p = 0.003 and OR = 0.568, p = 0.004, respectively). However, for TLR9 rs352140, patients who carried genotype TT and minor allele T developed seizures more often than those without (OR = 3.385, p = 0.004 and OR = 1.767, p = 0.004, respectively). Our finding suggested that genetic variations in TLR2 and TLR9 are associated with severity and prognosis of bacterial meningitis in Chinese children. However, the results should be interpreted with caution since the number of subjects included was limited.
authors.affiliation: Department of Infectious Diseases, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China.
authors.forename: Pingping
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Medical Microbiology and Research Centre of Microbiome, Capital Medical University, Beijing 100069, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Department of Infectious Diseases, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Medical Microbiology and Research Centre of Microbiome, Capital Medical University, Beijing 100069, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zheng
authors.affiliation: Department of Infectious Diseases, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Medical Microbiology and Research Centre of Microbiome, Capital Medical University, Beijing 100069, China.
authors.forename: Junping
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Medical Microbiology and Research Centre of Microbiome, Capital Medical University, Beijing 100069, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Cao
authors.affiliation: Department of Medical Microbiology and Research Centre of Microbiome, Capital Medical University, Beijing 100069, China.
authors.forename: Yiyuan
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Infectious Diseases, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Medical Microbiology and Research Centre of Microbiome, Capital Medical University, Beijing 100069, China.|Department of Medical Microbiology and Immunology, University of Turku, Turku 20520, Finland.
authors.forename: Qiushui
authors.initials: Q
authors.lastname: He
mesh_terms: D000293:Adolescent; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D007223:Infant; D008297:Male; D016920:Meningitis, Bacterial / Q000150:complications / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D012640:Seizures / Q000209:etiology*; D051195:Toll-Like Receptor 2 / Q000235:genetics*; D051217:Toll-Like Receptor 9 / Q000235:genetics*
chemical_list: C495342:TLR2 protein, human; C495371:TLR9 protein, human; D051195:Toll-Like Receptor 2; D051217:Toll-Like Receptor 9