title: The equilibrium of ubiquitination and deubiquitination at PLK1 regulates sister chromatid separation.
abstract: PLK1 regulates almost every aspect of mitotic events, including mitotic entry, spindle assembly, chromosome alignment, sister chromatid segregation, metaphase-anaphase transition, cytokinesis, etc. In regulating the chromosome alignment and sister chromatid segregation, PLK1 has to be localized to and removed from kinetochores at the right times, and the underlying mechanism that regulates PLK1 both spatially and temporally only became clearer recently. It has been found that deubiquitination and ubiquitination of PLK1 are responsible for its localization to and dissociation from the kinetochores, respectively. The equilibrium of this ubiquitination and deubiquitination plays an important role in regulating proper chromosome alignment and timely sister chromatid segregation. Here, we summarize and discuss the recent findings in investigating the spatial and temporal regulation of PLK1 during chromosome alignment and sister chromatid segregation.
authors.affiliation: Department of Biological Sciences, California State Polytechnic University, Pomona, CA, 91768, USA. junjunliu@cpp.edu.
authors.forename: Junjun
authors.initials: J
authors.lastname: Liu
authors.affiliation: The Ministry of Education Key Laboratory of Cell Proliferation and Differentiation and the State Key Laboratory of Membrane Biology, College of Life Sciences, Peking University, Beijing, 100871, China. zhangcm@pku.edu.cm.
authors.forename: Chuanmao
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002842:Chromatids / Q000378:metabolism*; D006801:Humans; D018386:Kinetochores / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D013379:Substrate Specificity; D054875:Ubiquitination; D000097869:Polo-Like Kinase 1
chemical_list: D018797:Cell Cycle Proteins; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D000097869:Polo-Like Kinase 1
keywords: Cullin 3; E3 ligase; Kinetochore; Mitosis; Polo-like kinase 1; Usp16