title: Reduced immunohistochemical expression of adhesion molecules in vitiligo skin biopsies.
abstract: Because defects in adhesion impairment seem to be involved in the etiopathogenesis of vitiligo, this study aimed to compare the immunohistochemical expression of several adhesion molecules in the epidermis of vitiligo and non lesional vitiligo skin. Sixty-six specimens of lesional and non lesional skin from 33 volunteers with vitiligo were evaluated by immunohistochemistry using anti-beta-catenin, anti-E-cadherin, anti-laminin, anti-beta1 integrin, anti-collagen IV, anti-ICAM-1 and anti-VCAM-1 antibodies. Biopsies of vitiligo skin demonstrated a significant reduction in the expression of laminin and integrin. The average value of the immunohistochemically positive reaction area of the vitiligo specimens was 3053.2μm2, compared with the observed value of 3431.8μm2in non vitiligo skin (p=0.003) for laminin. The immuno-positive area was 7174.6μm2(vitiligo) and 8966.7μm2(non lesional skin) for integrin (p=0.042). A reduction in ICAM-1 and VCAM-1 expression in the basal layer of the epidermis in vitiligo samples was also observed (p=0.001 and p<0.001, respectively). However, no significant differences were observed with respect to the expression of beta-catenin, E-cadherin, and collagen IV between vitiligo and non lesional skin. Our results suggest that an impairment in adhesion exists in vitiligo skin, which is supported by the diminished immunohistochemical expression of laminin, beta1 integrin, ICAM-1 and VCAM-1.
authors.affiliation: Santa Casa de Misericórdia de Curitiba Hospital, Praça Rui Barbosa, 694-Centro, Curitiba, PR, CEP 80010-030, Brazil; Experimental Pathology Laboratory, School of Medicine, Pontifical Catholic University of Paraná, Rua Imaculada Conceição, 1155 - Prado Velho, Curitiba, PR, CEP 80215-901, Brazil. Electronic address: adri_reichert@yahoo.com.br.
authors.forename: Adriane
authors.initials: A
authors.lastname: Reichert Faria
authors.affiliation: Erasto Gaertner Hospital, Curitiba, PR, Brazil.
authors.forename: Juliana Elizabeth
authors.initials: JE
authors.lastname: Jung
authors.affiliation: Santa Casa de Misericórdia de Curitiba Hospital, Praça Rui Barbosa, 694-Centro, Curitiba, PR, CEP 80010-030, Brazil.
authors.forename: Caio César
authors.initials: CC
authors.lastname: Silva de Castro
authors.affiliation: Experimental Pathology Laboratory, School of Medicine, Pontifical Catholic University of Paraná, Rua Imaculada Conceição, 1155 - Prado Velho, Curitiba, PR, CEP 80215-901, Brazil.
authors.forename: Lucia
authors.initials: L
authors.lastname: de Noronha
mesh_terms: D000328:Adult; D001706:Biopsy; D015820:Cadherins / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D024141:Collagen Type IV / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016023:Integrins / Q000378:metabolism*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007797:Laminin / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012867:Skin / Q000378:metabolism* / Q000473:pathology; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism; D014820:Vitiligo / Q000378:metabolism* / Q000473:pathology; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D015820:Cadherins; D015815:Cell Adhesion Molecules; D024141:Collagen Type IV; D016023:Integrins; D007797:Laminin; D019010:Vascular Cell Adhesion Molecule-1; D051176:beta Catenin; D018799:Intercellular Adhesion Molecule-1
keywords: Adhesion; Immunohistochemistry; Paraffin- embedded; Skin biopsy; Vitiligo