title: Toward Liquid Biopsy: Determination of the Humoral Immune Response in Cancer Patients Using HaloTag Fusion Protein-Modified Electrochemical Bioplatforms.
abstract: Autoantibodies raised against tumor-associated antigens have shown high promise as clinical biomarkers for reliable diagnosis, prognosis, and therapy monitoring of cancer. An electrochemical disposable biosensor for the specific and sensitive determination of p53-specific autoantibodies has been developed for the first time in this work. This biosensor involves the use of magnetic microcarriers (MBs) modified with covalently immobilized HaloTag fusion p53 protein as solid supports for the selective capture of specific autoantibodies. After magnetic capture of the modified MBs onto screen-printed carbon working electrodes, the amperometric signal using the system hydroquinone/H2O2was related to the levels of p53-autoantibodies in the sample. The biosensor was applied for the analysis of sera from 24 patients with high-risk of developing colorectal cancer and 6 from patients already diagnosed with colorectal (4) and ovarian (2) cancer. The developed biosensor was able to determine p53 autoantibodies with a sensitivity higher than that of a commercial standard ELISA using a just-in-time produced protein in a simpler protocol with less sample volume and easily miniaturized and cost-effective instrumentation.
authors.affiliation: Departamento de Bioquímica y Biología Molecular IV, Facultad de Óptica y Optometría, Universidad Complutense de Madrid , E-28040 Madrid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Garranzo-Asensio
authors.affiliation: Departamento de Bioquímica y Biología Molecular IV, Facultad de Óptica y Optometría, Universidad Complutense de Madrid , E-28040 Madrid, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Guzman-Aranguez
authors.affiliation: Gastroenterology Unit, Hospital Universitario Clínico San Carlos , E-28040 Madrid, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Povés
authors.affiliation: Surgical Pathology Department, Hospital Universitario Clínico San Carlos , E-28040 Madrid, Spain.
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Fernández-Aceñero
authors.affiliation: Departamento de Química Analítica, Facultad de Ciencias Químicas, Universidad Complutense de Madrid , 28040 Madrid, Spain.
authors.forename: Rebeca M
authors.initials: RM
authors.lastname: Torrente-Rodríguez
authors.affiliation: Departamento de Química Analítica, Facultad de Ciencias Químicas, Universidad Complutense de Madrid , 28040 Madrid, Spain.
authors.forename: Víctor
authors.initials: V
authors.lastname: Ruiz-Valdepeñas Montiel
authors.affiliation: Departamento de Medicina, Facultad de Medicina, Instituto de Investigaciones Biomédicas "Alberto Sols", CSIC-UAM , E-28029 Madrid, Spain.
authors.forename: Gemma
authors.initials: G
authors.lastname: Domínguez
authors.affiliation: Gynecology and Obstetrics Department, Hospital Puerta de Hierro , E-28222 Majadahonda, Spain.
authors.forename: Luis San
authors.initials: LS
authors.lastname: Frutos
authors.affiliation: Medical Oncology Department, Hospital Universitario La Paz , E-28046 Madrid, Spain.
authors.forename: Nuria
authors.initials: N
authors.lastname: Rodríguez
authors.affiliation: Departamento de Bioquímica y Biología Molecular I Facultad de Ciencias Químicas, Universidad Complutense de Madrid , E-28040 Madrid, Spain.
authors.forename: Mayte
authors.initials: M
authors.lastname: Villalba
authors.affiliation: Departamento de Química Analítica, Facultad de Ciencias Químicas, Universidad Complutense de Madrid , 28040 Madrid, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Pingarrón
authors.affiliation: Departamento de Química Analítica, Facultad de Ciencias Químicas, Universidad Complutense de Madrid , 28040 Madrid, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Campuzano
authors.affiliation: Departamento de Bioquímica y Biología Molecular I Facultad de Ciencias Químicas, Universidad Complutense de Madrid , E-28040 Madrid, Spain.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Barderas
mesh_terms: D001323:Autoantibodies / Q000097:blood* / Q000276:immunology*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D015179:Colorectal Neoplasms / Q000097:blood / Q000276:immunology; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D005260:Female; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000276:immunology; D056724:Immunity, Humoral; D057230:Limit of Detection; D000073890:Liquid Biopsy / Q000295:instrumentation / Q000379:methods; D008958:Models, Molecular; D009369:Neoplasms / Q000097:blood* / Q000276:immunology*; D010051:Ovarian Neoplasms / Q000097:blood / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000276:immunology; D016159:Tumor Suppressor Protein p53 / Q000737:chemistry / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D055767:Immobilized Proteins; D011993:Recombinant Fusion Proteins; D016159:Tumor Suppressor Protein p53