title: Preparation of Protein-containing Extracts from Microbiota-rich Intestinal Contents.
abstract: The contribution of microbiota in regulating multiple physiological and pathological host responses has been studied intensively in recent years. Evidence suggests that commensal microbiota can directly modulate different populations of cells of the immune system (e.g., Ivanovet al., 2008; Atarashiet al., 2011). Recently, we showed that protein extracts from gut commensal microbiota can activate retina-specific T cells, allowing these autoreactive T cells to then break through the blood-retinal barrier and trigger autoimmune uveitis in the recipient (Horaiet al., 2015). The protocol below describes the method to prepare intestinal protein-rich extracts that can be used in variousin vitroandin vivoimmunological studies.
authors.affiliation: Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, Florianopolis, Santa Catarina, Brazil; Oral and Pharyngeal Cancer Branch, National Institutes of Dental and Craniofacial Research, National Institutes of Health, NIH, Bethesda, MD, USA.
authors.forename: Patricia
authors.initials: P
authors.lastname: Dillenburg-Pilla
authors.affiliation: Laboratory of Immunoregulation, Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Florianopolis, Santa Catarina, Brazil;; Laboratory of Immunology, National Eye Institute, NIH, Bethesda, MD, USA.
authors.forename: Carlos R
authors.initials: CR
authors.lastname: Zárate-Bladés
authors.affiliation: Laboratory of Immunology, National Eye Institute, NIH, Bethesda, MD, USA.
authors.forename: Phyllis B
authors.initials: PB
authors.lastname: Silver
authors.affiliation: Laboratory of Immunology, National Eye Institute, NIH, Bethesda, MD, USA.
authors.forename: Reiko
authors.initials: R
authors.lastname: Horai
authors.affiliation: Laboratory of Immunology, National Eye Institute, NIH, Bethesda, MD, USA.
authors.forename: Rachel R
authors.initials: RR
authors.lastname: Caspi