title: Immobilization of 2-Deoxy-d-ribose-5-phosphate Aldolase in Polymeric Thin Films via the Langmuir-Schaefer Technique.
abstract: A synthetic protocol for the fabrication of ultrathin polymeric films containing the enzyme 2-deoxy-d-ribose-5-phosphate aldolase from Escherichia coli (DERAEC) is presented. Ultrathin enzymatically active films are useful for applications in which only small quantities of active material are needed and at the same time quick response and contact times without diffusion limitation are wanted. We show how DERA as an exemplary enzyme can be immobilized in a thin polymer layer at the air-water interface and transferred to a suitable support by the Langmuir-Schaefer technique under full conservation of enzymatic activity. The polymer in use is a poly(N-isopropylacrylamide-co-N-2-thiolactone acrylamide) (P(NIPAAm-co-TlaAm)) statistical copolymer in which the thiolactone units serve a multitude of purposes including hydrophobization of the polymer, covalent binding of the enzyme and the support and finally cross-linking of the polymer matrix. The application of this type of polymer keeps the whole approach simple as additional cocomponents such as cross-linkers are avoided.
authors.affiliation: Department of Functional Protein Systems and Biotechnology, Fraunhofer Institute of Applied Polymer Research (IAP) , Geiselbergstraße 69, 14476, Potsdam-Golm, Germany.
authors.forename: Stefan
authors.identifier: http://orcid.org/0000-0002-3666-6504
authors.initials: S
authors.lastname: Reinicke
authors.affiliation: Department of Chemistry, University of Chicago , Chicago, Illinois 60637, United States.
authors.forename: Huw C
authors.initials: HC
authors.lastname: Rees
authors.affiliation: Department of Organic and Macromolecular Chemistry, Polymer Chemistry Research Group, Ghent University , Krijgslaan 281 S4-bis, 9000 Ghent, Belgium.
authors.forename: Pieter
authors.initials: P
authors.lastname: Espeel
authors.affiliation: Department of Pharmaceutics, Ghent University , Ottergemsesteenweg 460, 9000 Ghent, Belgium.
authors.forename: Nane
authors.initials: N
authors.lastname: Vanparijs
authors.affiliation: Institut of Bioorganic Chemistry, Heinrich Heine University of Düsseldorf at Forschungszentrum Jülich , Stetternicher Forst, D-52426 Jülich, Germany.
authors.forename: Carolin
authors.initials: C
authors.lastname: Bisterfeld
authors.affiliation: Institut of Bioorganic Chemistry, Heinrich Heine University of Düsseldorf at Forschungszentrum Jülich , Stetternicher Forst, D-52426 Jülich, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Dick
authors.affiliation: Department of Functional Protein Systems and Biotechnology, Fraunhofer Institute of Applied Polymer Research (IAP) , Geiselbergstraße 69, 14476, Potsdam-Golm, Germany.|Polymer Materials and Polymer Technologies, University of Potsdam , 14476, Potsdam-Golm, Germany.
authors.forename: Ruben R
authors.initials: RR
authors.lastname: Rosencrantz
authors.affiliation: Max Planck Institute of Colloids and Interfaces , Science Park Potsdam-Golm, Am Mühlenberg 1, 14476 Potsdam, Germany.
authors.forename: Gerald
authors.initials: G
authors.lastname: Brezesinski
authors.affiliation: Department of Pharmaceutics, Ghent University , Ottergemsesteenweg 460, 9000 Ghent, Belgium.
authors.forename: Bruno G
authors.initials: BG
authors.lastname: de Geest
authors.affiliation: Department of Organic and Macromolecular Chemistry, Polymer Chemistry Research Group, Ghent University , Krijgslaan 281 S4-bis, 9000 Ghent, Belgium.
authors.forename: Filip E
authors.identifier: http://orcid.org/0000-0003-4065-6373
authors.initials: FE
authors.lastname: Du Prez
authors.affiliation: Institut of Bioorganic Chemistry, Heinrich Heine University of Düsseldorf at Forschungszentrum Jülich , Stetternicher Forst, D-52426 Jülich, Germany.|IBG-1: Biotechnology, Forschungszentrum Jülich GmbH , 52425 Jülich, Germany.
authors.forename: Jörg
authors.identifier: http://orcid.org/0000-0002-9819-889X
authors.initials: J
authors.lastname: Pietruszka
authors.affiliation: Department of Functional Protein Systems and Biotechnology, Fraunhofer Institute of Applied Polymer Research (IAP) , Geiselbergstraße 69, 14476, Potsdam-Golm, Germany.|Polymer Materials and Polymer Technologies, University of Potsdam , 14476, Potsdam-Golm, Germany.
authors.forename: Alexander
authors.identifier: http://orcid.org/0000-0002-5760-6631
authors.initials: A
authors.lastname: Böker
keywords: 2-deoxy-d-ribose-5-phosphate aldolase; Langmuir−Schaefer; enzyme immobilization; poly(N-isopropylacrylamide); polymeric thin film; thiolactone