title: Intracellular and in Vivo Cyanide Mapping via Surface Plasmon Spectroscopy of Single Au-Ag Nanoboxes.
abstract: Cyanide is extremely toxic to organisms but difficult to detect in living biological specimens. Here, we report a new CN-sensing platform based on unmodified Au-Ag alloy nanoboxes that etch in the presence of this analyte, yielding a shift in plasmon frequency that correlates with the analyte concentration. Significantly, when combined with dark field microscopy, these particle probes can be used to measure CN-concentrations in HeLa cells and in vivo in Zebra fish embryos. The limit of detection (LOD) of the novel method is 1 nM (below the acceptable limit defined by the World Health Organization), and finite-difference time-domain (FDTD) calculations are used to understand the CN-induced spectral shifts.
authors.affiliation: Department of Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, State Key Laboratory of Molecular Engineering of Polymers, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Peiyuan
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Physics, Key Laboratory of Micro and Nano Photonic Structures (MOE) and Key Laboratory of Surface Physics, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Department of Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, State Key Laboratory of Molecular Engineering of Polymers, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Chi
authors.initials: C
authors.lastname: Yao
authors.affiliation: Department of Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, State Key Laboratory of Molecular Engineering of Polymers, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, State Key Laboratory of Molecular Engineering of Polymers, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, State Key Laboratory of Molecular Engineering of Polymers, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Wenxing
authors.initials: W
authors.lastname: Wang
authors.affiliation: King Abdullah Institute for Nanotechnology, King Saud University, Riyadh 11451, Saudi Arabia.|Central Metallurgical Research and Development Institute, CMRDI , Helwan, Cairo 11421, Egypt.
authors.forename: Ahmed Mohamed
authors.initials: AM
authors.lastname: El-Toni
authors.affiliation: Department of Physics, Key Laboratory of Micro and Nano Photonic Structures (MOE) and Key Laboratory of Surface Physics, Fudan University , Shanghai 200433, P. R. China.|Collaborative Innovation Center of Advanced Microstructures, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zi
authors.affiliation: Department of Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, State Key Laboratory of Molecular Engineering of Polymers, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Dongyuan
authors.identifier: 0000-0001-8440-6902
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Department of Physics, Key Laboratory of Micro and Nano Photonic Structures (MOE) and Key Laboratory of Surface Physics, Fudan University , Shanghai 200433, P. R. China.|Collaborative Innovation Center of Advanced Microstructures, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.affiliation: Department of Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, State Key Laboratory of Molecular Engineering of Polymers, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Fan
authors.identifier: 0000-0001-7886-6144
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000497:Alloys / Q000737:chemistry; D000818:Animals; D003486:Cyanides / Q000032:analysis*; D004625:Embryo, Nonmammalian / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D057230:Limit of Detection; D049329:Nanostructures / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D020349:Surface Plasmon Resonance; D015027:Zebrafish / Q000254:growth & development
chemical_list: D000497:Alloys; D003486:Cyanides; D012834:Silver; D006046:Gold