title: Inonotus obliquus polysaccharide regulates gut microbiota of chronic pancreatitis in mice.
abstract: Polysaccharide is efficient in attenuation of metabolic ailments and modulation of gut microbiota as prebiotics. The therapeutic effect of Inonotus obliquus polysaccharide (IOP) on chronic pancreatitis (CP) in mice has been validated in our previous study. However, it is not clear whether IOP is conducive to maintaining the homeostasis between gut microbiota and host. The aim of this study is to testify the potential effects of IOP on gut microbiota composition and diversity in mice with CP. The changes in glutathione peroxidase (GSH-PX), total antioxidant capacity (TAOC), tumor necrosis factor alpha (TNF-α), transforming growth factor beta (TGF-β), lipase and trypsin levels were measured by commercial assay kits, meanwhile the gut microbiota composition and diversity were analyzed by high throughput sequencing. The IOP treatment increased GSH-PXand TAOC levels, and decreased TNF-α, TGF-β, lipase and trypsin levels in CP mice. It was also observed that gut microbiota in IOP treated groups were less diverse than others in terms of lower Shannon diversity index and Chao 1 estimator. IOP increased the proportion of Bacteroidetes and decreased that of Firmicutes at phylum level. Bacteroidetes was found positively correlated with GSH-PXand TAOC, and Firmicutes correlated with TNF-α, TGF-β, and lipase. In conclusion, administration of IOP could regulate gut microbiota composition and diversity to a healthy profile in mice with CP, and some bacterial phylum significantly correlated with characteristic parameters.
authors.affiliation: College of Resources and Environmental Science, Northeast Agricultural University, Harbin, 150030, People's Republic of China.|School of Life Sciences, Tsinghua University, Beijing, 100084, People's Republic of China.|The Shenzhen Key Laboratory of Gene and Antibody Therapy, State Key Laboratory of Health Science and Technology (prep), Center for Biotechnology & Biomedicine and Division of Life & Health Sciences, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Hu
authors.affiliation: College of Resources and Environmental Science, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Chunying
authors.initials: C
authors.lastname: Teng
authors.affiliation: College of Resources and Environmental Science, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Sumei
authors.initials: S
authors.lastname: Yu
authors.affiliation: College of Resources and Environmental Science, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Resources and Environmental Science, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Liang
authors.affiliation: College of Resources and Environmental Science, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Bai
authors.affiliation: College of Resources and Environmental Science, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Liying
authors.initials: L
authors.lastname: Dong
authors.affiliation: College of Resources and Environmental Science, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Song
authors.affiliation: Drug Safety Evaluation Center, Heilongjiang University of Chinese Medicine, Harbin, 150040, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Yu
authors.affiliation: College of Resources and Environmental Science, Northeast Agricultural University, Harbin, 150030, People's Republic of China. juanjuan4050234@163.com.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Qu
keywords: Chronic pancreatitis; Gut microbiota; High throughput sequencing; Inonotus obliquus polysaccharide; Mice