title: Desulfobulbus aggregans sp. nov., a Novel Sulfate Reducing Bacterium Isolated from Marine Sediment from the Gulf of Gabes.
abstract: Three sulfate-reducing bacterial strains designated SM40T, SM41, and SM43 were isolated from marine sediment in the region of Skhira located in the Gulf of Gabes (Tunisia). These strains grew in anaerobic media with phosphogypsum as a sulfate source and sodium lactate as an electron and carbon source. One of them, strain SM40T, was characterized by phenotypic and phylogenetic methods. Cells were ovoid, Gram-stain-negative and non-motile. The temperature limits for growth were 10 and 55 °C with an optimum at 35 °C and the pH range was 6.5-8.1 with an optimum at pH 7.5. Growth was observed at salinities ranging from 10 to 80 g NaCl l-1with an optimum at 30 g NaCl l-1. Strain SM40Twas able to utilize butanol, ethanol, formate, L-glucose, glycerol, lactate, propanol, propionate, and pyruvate as electron donors for the reduction of sulfate, sulfite, or thiosulfate to H2S. Without electron acceptors, strain SM40Tfermented butanol and pyruvate. The DNA G+C content of strain SM40Twas 52.6 mol %. Phylogenetic analysis based on the 16S rRNA gene sequence of the isolate revealed that strain SM40Twas closely related to the species in the genus Desulfobulbus of the family Desulfobulbaceae. The sequence similarity between strain SM40 and Desulfobulbus marinus was 95.4%. The phylogenetic analysis, DNA G+C content, and differences in substrate utilization suggested that strain SM40 represents a new species of the genus Desulfobulbus, D. aggregans sp. nov. The type strain is strain SM40T(=DSM 28693T= JCM 19994T).
authors.affiliation: Laboratoire des Bioprocédés Environnementaux, Laboratoire Mixte International (LMI) (Cosys-Med), Centre de Biotechnologie de Sfax, Sidi Mansour, Km 6, BP 1177, 3018, Sfax, Tunisia.
authors.forename: Hanen
authors.initials: H
authors.lastname: Kharrat
authors.affiliation: Laboratoire des Bioprocédés Environnementaux, Laboratoire Mixte International (LMI) (Cosys-Med), Centre de Biotechnologie de Sfax, Sidi Mansour, Km 6, BP 1177, 3018, Sfax, Tunisia. fatma.karray@cbs.rnrt.tn.
authors.forename: Fatma
authors.initials: F
authors.lastname: Karray
authors.affiliation: Laboratoire de Microbiologie IRD, Aix-Marseille University, Université de Toulon, CNRS, IRD, MIO, 13288, Marseille Cedex 09, France.
authors.forename: Manon
authors.initials: M
authors.lastname: Bartoli
authors.affiliation: Laboratoire de Microbiologie IRD, Aix-Marseille University, Université de Toulon, CNRS, IRD, MIO, 13288, Marseille Cedex 09, France.
authors.forename: Wajdi
authors.initials: W
authors.lastname: Ben Hnia
authors.affiliation: Laboratoire des Bioprocédés Environnementaux, Laboratoire Mixte International (LMI) (Cosys-Med), Centre de Biotechnologie de Sfax, Sidi Mansour, Km 6, BP 1177, 3018, Sfax, Tunisia.
authors.forename: Najla
authors.initials: N
authors.lastname: Mhiri
authors.affiliation: Laboratoire de Microbiologie IRD, Aix-Marseille University, Université de Toulon, CNRS, IRD, MIO, 13288, Marseille Cedex 09, France.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Fardeau
authors.affiliation: Laboratoire de recherche, Groupe Chimique Tunisien, Centre de Recherche Scientifique de Sfax, BP «S », 3018, Sfax, Tunisia.
authors.forename: Feten
authors.initials: F
authors.lastname: Bennour
authors.affiliation: Laboratoire de recherche, Groupe Chimique Tunisien, Centre de Recherche Scientifique de Sfax, BP «S », 3018, Sfax, Tunisia.
authors.forename: Lotfi
authors.initials: L
authors.lastname: Kamoun
authors.affiliation: Laboratoire des Bioprocédés Environnementaux, Laboratoire Mixte International (LMI) (Cosys-Med), Centre de Biotechnologie de Sfax, Sidi Mansour, Km 6, BP 1177, 3018, Sfax, Tunisia.|Laboratoire de Microbiologie IRD, Aix-Marseille University, Université de Toulon, CNRS, IRD, MIO, 13288, Marseille Cedex 09, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Alazard
authors.affiliation: Laboratoire des Bioprocédés Environnementaux, Laboratoire Mixte International (LMI) (Cosys-Med), Centre de Biotechnologie de Sfax, Sidi Mansour, Km 6, BP 1177, 3018, Sfax, Tunisia.
authors.forename: Sami
authors.initials: S
authors.lastname: Sayadi
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D019015:Geologic Sediments / Q000382:microbiology*; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology; D016999:Sulfur-Reducing Bacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S