title: Pilose antler peptide protects osteoblasts from inflammatory and oxidative injury through EGF/EGFR signaling.
abstract: Epidermal growth factor (EGF)/EFG receptor (EGFR) signaling plays an important role in the osteoblastogenesis. The potential effects of pilose antler peptide (PAP) on osteoblast cell damages was investigated in our present study through EGF/EGFR signaling. In MC3T3-E1 osteoblastic cells, PAP treatment significantly inhibited the production of inflammatory cytokines by decreasing the levels of serum proinflammatory cytokines interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). PAP treatment also alleviated the oxidative responses as indicated by increased activities of catalase (SOD) and decreased levels of malondialdehyde (MDA). EGF inhibition, by siRNA knockdown, almost abolished PAP-induced osteoblast cytoprotection against inflammation and oxidant stress. Further, our results showed that PAP stimulated the nuclear erythroid factor 2-related factor 2 (Nrf2)2/heme oxygenase-1(HO-1) signaling, and inhibited the activation of uclear factor kappa B (NF-κB) pathway in MC3T3-E1 cells. On the other hand, EGF siRNA knockdown inhibited PAP-induced cytoprotection, which decreased the expression of Nrf-2, HO-1 and increased the level of p-NF-κBp65, p-IκBα in MC3T3-E1 cells. Thus, our research demonstrated that PAP protects osteoblasts from inflammatory and oxidative injury through EGF/EGFR signaling.
authors.affiliation: The first affiliated hospital of Changchun University of Chinese Medicine, Changhcun, 130117, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chunhui
authors.affiliation: The first affiliated hospital of Changchun University of Chinese Medicine, Changhcun, 130117, China.
authors.forename: Cai
authors.initials: C
authors.lastname: Wenjun
authors.affiliation: The first affiliated hospital of Changchun University of Chinese Medicine, Changhcun, 130117, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Hui
authors.affiliation: The first affiliated hospital of Changchun University of Chinese Medicine, Changhcun, 130117, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Liquan
authors.affiliation: The first affiliated hospital of Changchun University of Chinese Medicine, Changhcun, 130117, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Changwei
authors.affiliation: Changchun University of Chinese Medicine, Changhcun, 130117, China. Electronic address: zhaowenhaitcm@126.com.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Tianzhu
authors.affiliation: The first affiliated hospital of Changchun University of Chinese Medicine, Changhcun, 130117, China. Electronic address: zhangtz452@126.com.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Wenhai
mesh_terms: D000818:Animals; D000999:Antlers / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000097:blood; D019610:Cytoprotection / Q000187:drug effects; D003670:Deer / Q000033:anatomy & histology; D004815:Epidermal Growth Factor / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism*; D007249:Inflammation / Q000097:blood / Q000517:prevention & control; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010455:Peptides / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D016207:Cytokines; D010455:Peptides; D008315:Malondialdehyde; D004815:Epidermal Growth Factor; D013482:Superoxide Dismutase; D066246:ErbB Receptors
keywords: EGF/EGFR signaling; Inflammation; Osteoblasts; Pilose antler peptide