title: Lack of association between integrin αvβ3gene polymorphisms and hemorrhagic fever with renal syndrome in Han Chinese from Hubei, China.
abstract: Hantaviruses belong to the family Bunyaviridae and cause hemorrhagic fever with renal syndrome (HFRS) in humans. β3integrins, including αVβ3and αIIbβ3integrins, act as receptors on endothelial cells and play key roles in cellular entry during the pathogenesis of hantaviruses. Previous study demonstrated that the polymorphisms of integrin αIIbβ3are associated with susceptibility to hantavirus infection and the disease severity of HFRS in Shaanxi Province of China, rather than in Finland. However, the polymorphisms of integrin αvβ3in patients with HFRS was incompletely understood. Here, we aimed to investigate the associations between polymorphisms in human integrin αvβ3and HFRS in Han Chinese individuals. Ninety patients with HFRS and 101 healthy controls were enrolled in this study. Analysis of five single nucleotide polymorphism (SNP) sites (rs3768777 and rs3738919 on ITGAV; rs13306487, rs5921, and rs5918 on ITGB3) was performed by TaqMan SNP genotyping assays and bi-directional PCR allele-specific amplification method. No significant differences were observed between the HFRS group and controls regarding the genotype and allele frequency distributions of any of the five SNP sites, and no associations were found between ITGAV polymorphisms/genotypes and disease severity. In conclusion, our results implied that these five SNPs in the integrin αvβ3gene were not associated with HFRS susceptibility or severity in Han Chinese individuals in Hubei Province.
authors.affiliation: State Key Laboratory of Virology, Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University, Wuhan, 430071, China.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Virology, Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University, Wuhan, 430071, China.
authors.forename: Hai-Rong
authors.initials: HR
authors.lastname: Xiong
authors.affiliation: State Key Laboratory of Virology, Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University, Wuhan, 430071, China.
authors.forename: Ni
authors.initials: N
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Virology, Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University, Wuhan, 430071, China.
authors.forename: Qing-Zhou
authors.initials: QZ
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Virology, Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University, Wuhan, 430071, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Virology, Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University, Wuhan, 430071, China.
authors.forename: Chao-Jie
authors.initials: CJ
authors.lastname: Zhong
authors.affiliation: State Key Laboratory of Virology, Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University, Wuhan, 430071, China.
authors.forename: Mei-Rong
authors.initials: MR
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Virology, Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University, Wuhan, 430071, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Virology, Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University, Wuhan, 430071, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Virology, Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University, Wuhan, 430071, China. houwei@whu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Hou
mesh_terms: D000328:Adult; D044466:Asian People; D002681:China; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D060005:Genotyping Techniques; D006480:Hemorrhagic Fever with Renal Syndrome / Q000235:genetics*; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
chemical_list: D039302:Integrin alphaVbeta3
keywords: hantavirus; hemorrhagic fever with renal syndrome (HFRS); integrin αvβ3; polymorphisms