title: Correlation between the GP78 Gene Polymorphism and Coronary Atherosclerotic Heart Disease.
abstract: OBJECTIVE
  To study the correlation between the GP78 gene polymorphism and blood fat, blood glucose, blood pressure and coronary atherosclerotic heart disease.
  
  
  METHODS
  A total of 72 patients with coronary atherosclerotic heart disease were selected as the observation group, and 68 healthy participants were selected as the control group. The gp78 gene polymorphism of both groups was studied via polymerase chain reaction-restriction fragment length polymorphism (RFLP). At the same time, the multiple expression quantities of the GP78 gene in the tissues of both groups were tested via fluorogenic quantitative PCR, enzyme-linked immunosorbent assay (ELISA) and Western-blotting assay. Furthermore, the blood fat, blood glucose and blood pressure of subjects in both groups were tested.
  
  
  RESULTS
  The percentages of the gp78 gene polymorphisms of Arg/Arg, Arg/Gly and Gly/Gly at the 145 locus of the study subjects in the observation group were 12.3%, 43.2% and 44.5%, respectively, while those in the control group were 74.3%, 11.2% and 14.5%, respectively, and there were significant differences between both groups. Based on the test results of the blood fat, blood glucose and blood pressure of the objects in the observation group and control group, significant differences were found between the two groups (P<0.05).
  
  
  CONCLUSIONS
  There was a significant correlation between the 145 locus of the gp89 gene and coronary atherosclerotic heart disease, indexes of blood fat, blood glucose and blood pressure.
authors.affiliation: Department of Geriatrics, The First Hospital of Lanzhou University, China. Electronic address: ardorien@126.com.
authors.forename: Long
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Cardiovascular Medicine, Xigu Branch, Lanzhou University Second Hospital, China.
authors.forename: Hua-Cheng
authors.initials: HC
authors.lastname: Li
authors.affiliation: Department of Cardiovascular Medicine, Gansu Provincial Hospital of TCM, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Cardiovascular Medicine, The First Hospital of Lanzhou University, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Geriatrics, The First Hospital of Lanzhou University, China.
authors.forename: Cheng-Dong
authors.initials: CD
authors.lastname: Qiao
authors.affiliation: Department of Geriatrics, The First Hospital of Lanzhou University, China.
authors.forename: Yong-Ming
authors.initials: YM
authors.lastname: Liu
authors.affiliation: Department of Cardiovascular Medicine, The First Hospital of Lanzhou University, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000328:Adult; D050197:Atherosclerosis / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D003324:Coronary Artery Disease / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005500:Follow-Up Studies; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D060285:Receptors, Autocrine Motility Factor / Q000235:genetics* / Q000378:metabolism; D012189:Retrospective Studies
chemical_list: D004247:DNA; C555000:AMFR protein, human; D060285:Receptors, Autocrine Motility Factor
keywords: blood fat; blood glucose; blood pressure; coronary sclerosis; heart disease