title: Expression profile of biomarkers altered in papillary and anaplastic thyroid carcinoma: Contribution of Tunisian patients.
abstract: OBJECTIVE
  The objective of this study was to compare the protein expression profile between well-differentiated (papillary) and undifferentiated (anaplastic) thyroid carcinoma in Tunisian patients.
  
  
  METHODS
  This first Tunisian retrospective study concerned data of 38 thyroid cancer cases (19 papillary carcinoma PTC and 19 anaplastic carcinoma ATC) collected at Salah Azaiez Institute of Tunisia. Immunohistochemistry was used to evaluate tumor expression of different molecular markers (p53, Ki67, E-cadherin, cyclin D1, bcl2, S100 and Her-2). The molecular expression was correlated with the clinicopathological characteristics of the tumors.
  
  
  RESULTS
  There were 6 differentially expressed markers when comparing anaplastic thyroid carcinoma ATC with papillary thyroid carcinoma PTC. Expression of p53 and Ki67 were significantly increased in 16 and 18 ATC cases respectively, the Ki67 expression was lost in PTC. Cyclin D1, E-cadherin, bcl2 and S100 were overexpressed in PTC tumors; however, they were significantly decreased in ATC. The last marker, Her-2 was expressed in one case of PTC only.
  
  
  CONCLUSIONS
  Our results, similar with findings of other ethnic groups, showed alteration in expression of molecular markers associated with tumor dedifferentiation, indicating loss of cell cycle control with increased proliferative activity in ATC carcinoma. These data support the hypothesis that ATC may derive from dedifferentiation of preexisting PTC tumor.
authors.affiliation: Salah Azaiez Cancer Institute, Department of Immuno-Histo-Cytology Salah Azaiez, Place Bab Saadoun, 1006 Tunis, Tunisia. Electronic address: asma.fourati@rns.tn.
authors.forename: Asma
authors.initials: A
authors.lastname: Fourati
authors.affiliation: Salah Azaiez Cancer Institute, Department of Immuno-Histo-Cytology Salah Azaiez, Place Bab Saadoun, 1006 Tunis, Tunisia.
authors.forename: Olfa
authors.initials: O
authors.lastname: El Amine
authors.affiliation: Salah Azaiez Cancer Institute, Department of Epidemiology, Place Bab Saadoun, 1006 Tunis, Tunisia.
authors.forename: Wided
authors.initials: W
authors.lastname: Ben Ayoub
authors.affiliation: Salah Azaiez Cancer Institute, Department of Immuno-Histo-Cytology Salah Azaiez, Place Bab Saadoun, 1006 Tunis, Tunisia.
authors.forename: Imen
authors.initials: I
authors.lastname: Cherni
authors.affiliation: Salah Azaiez Cancer Institute, Department of Immuno-Histo-Cytology Salah Azaiez, Place Bab Saadoun, 1006 Tunis, Tunisia.
authors.forename: Aida
authors.initials: A
authors.lastname: Goucha
authors.affiliation: University of Tunis El Manar, Faculty of Medicine, Research unit 01/UR/08-07, Tunis, Tunisia.
authors.forename: Michèle Véronique
authors.initials: MV
authors.lastname: El May
authors.affiliation: Salah Azaiez Cancer Institute, Department of Immuno-Histo-Cytology Salah Azaiez, Place Bab Saadoun, 1006 Tunis, Tunisia.
authors.forename: Amor
authors.initials: A
authors.lastname: Gamoudi
authors.affiliation: Salah Azaiez Cancer Institute, Department of Immuno-Histo-Cytology Salah Azaiez, Place Bab Saadoun, 1006 Tunis, Tunisia.
authors.forename: Ahmed
authors.initials: A
authors.lastname: El May
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015820:Cadherins / Q000378:metabolism; D002291:Carcinoma, Papillary / Q000378:metabolism* / Q000473:pathology; D019938:Cyclin D1 / Q000378:metabolism; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism; D012189:Retrospective Studies; D009418:S100 Proteins / Q000378:metabolism; D065646:Thyroid Carcinoma, Anaplastic / Q000378:metabolism* / Q000473:pathology; D013964:Thyroid Neoplasms / Q000378:metabolism* / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014416:Tunisia
chemical_list: D014408:Biomarkers, Tumor; D015820:Cadherins; D019938:Cyclin D1; D019394:Ki-67 Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; D018719:Erb-b2 Receptor Tyrosine Kinases; D009418:S100 Proteins; D016159:Tumor Suppressor Protein p53; C000595707:BCL2 protein, human; C508053:ERBB2 protein, human
keywords: Anaplastic and papillary thyroid carcinoma; Carcinomes anaplasiques et papillaires de la thyroïde; Immunohistochemistry; Immunohistochimie; Marqueurs moléculaires; Molecular marker