title: Upregulated miR-483-5p expression as a prognostic biomarker for esophageal squamous cell carcinoma.
abstract: BACKGROUND
  Esophageal squamous cell carcinoma (ESCC) is one of the most common cause of cancer-associated mortality. Uncovering novel molecular biomarkers that can predict ESCC development will improve personalized therapy.
  
  
  OBJECTIVE
  The goal of the current study was to investigate the expression pattern of miR-483-5p and determine its prognostic value in ESCC.
  
  
  METHODS
  We first analyzed miRNA-seq data obtained from the Cancer Genome Atlas (TCGA) cohort to evaluate the prognostic value of miR-483-5p in ESCC. Then quantitative real-time RT-PCR (qRT-PCR) was carried out to compare the miR-483-5p levels in 80 pairs of ESCC tissues and adjacent non-cancerous tissues. The correlation between miR-483-5p levels and clinical features were determined.
  
  
  RESULTS
  For the TCGA cohort, ESCC patients with higher miR-483-5p had significantly shorter overall survival time. The examined ESCC cancer tissues exhibited a remarkable increment in miR-483-5p expression compared with the adjacent normal tissues. miR-483-5p was positively correlated with TNM stage, lymph nodes metastasis and T stage. In addition, upregulate miR-483-5p expression was also found to be significantly associated with poor survival of ESCC patients. Furthermore, miR-483-5p expression was an independent prognostic factor for overall survival and disease free survival in ESCC patients.
  
  
  CONCLUSIONS
  Our study demonstrates that miR-483-5p might be a tumor promoter of ESCC, which provide a promising prognostic biomarker and therapeutic target.
authors.affiliation: Southern Medical University, Guangzhou, Guangdong, China.|Department of Medical Oncology, Inner Mongolia Autonomous Region People's Hospital, Hohhot, China.
authors.forename: Liying
authors.initials: L
authors.lastname: Xue
authors.affiliation: Department of Heart Center, Inner Mongolia Autonomous Region People's Hospital, Hohhot, China.
authors.forename: Jinglong
authors.initials: J
authors.lastname: Nan
authors.affiliation: Department of Medical Oncology, Inner Mongolia Autonomous Region People's Hospital, Hohhot, China.
authors.forename: Li
authors.initials: L
authors.lastname: Dong
authors.affiliation: Department of Medical Oncology, Inner Mongolia Autonomous Region People's Hospital, Hohhot, China.
authors.forename: Cuiying
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Medical Oncology, Inner Mongolia Autonomous Region People's Hospital, Hohhot, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Medical Oncology, Inner Mongolia Autonomous Region People's Hospital, Hohhot, China.
authors.forename: Rentuya
authors.initials: R
authors.lastname: Na
authors.affiliation: Department of Pathology, Inner Mongolia Autonomous Region People's Hospital, Hohhot, China.
authors.forename: Huijie
authors.initials: H
authors.lastname: He
authors.affiliation: Southern Medical University, Guangzhou, Guangdong, China.|Department of Radiation Oncology, Army General Hospital of PLA, Beijing, China.
authors.forename: Yadi
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000556:secondary / Q000628:therapy; D015331:Cohort Studies; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D011379:Prognosis; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; C548912:MIRN483 microRNA, human; D035683:MicroRNAs
keywords: Biomarker; esophageal squamous cell carcinoma; miR-483-5p; prognosis 