title: Characterization of metabolites in different kiwifruit varieties by NMR and fluorescence spectroscopy.
abstract: It is known from our previous studies that kiwifruits, which are used in common human diet, have preventive properties of coronary artery disease. This study describes a combination of1H NMR spectroscopy, multivariate data analyses and fluorescence measurements in differentiating of some kiwifruit varieties, their quenching and antioxidant properties. A total of 41 metabolites were identified by comparing with literature data Chenomx database and 2D NMR. The binding properties of the extracted polyphenols against HSA showed higher reactivity of studied two cultivars in comparison with the common Hayward. The results showed that the fluorescence of HSA was quenched by Bidan as much as twice than by other fruits. The correlation between the binding properties of polyphenols in the investigated fruits, their relative quantification and suggested metabolic pathway was established. These results can provide possible application of fruit extracts in pharmaceutical industry.
authors.affiliation: Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Nur Ashikin
authors.initials: NA
authors.lastname: Abdul Hamid
authors.affiliation: Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Mediani
authors.affiliation: Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: M
authors.initials: M
authors.lastname: Maulidiani
authors.affiliation: Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. Electronic address: faridah_abas@upm.edu.my.
authors.forename: Faridah
authors.initials: F
authors.lastname: Abas
authors.affiliation: Department of Horticultural Science, Mokpo National University, Muan, Jeonnam, South Korea.
authors.forename: Yong Seo
authors.initials: YS
authors.lastname: Park
authors.affiliation: Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Warsaw, Poland.
authors.forename: Hanna
authors.initials: H
authors.lastname: Leontowicz
authors.affiliation: Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Warsaw, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Leontowicz
authors.affiliation: Gdańsk University of Technology, Chemical Faculty, Department of Analytical Chemistry, Gdańsk 80 952, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Namiesnik
authors.affiliation: Institute for Drug Research, School of Pharmacy, Hadassah Medical School, The Hebrew University, Jerusalem 91120, Israel. Electronic address: shela.gorin@mail.huji.ac.il.
authors.forename: S
authors.initials: S
authors.lastname: Gorinstein
mesh_terms: D029042:Actinidia / Q000737:chemistry* / Q000378:metabolism*; D000975:Antioxidants / Q000737:chemistry; D005638:Fruit / Q000737:chemistry* / Q000378:metabolism*; D008279:Magnetic Resonance Imaging / Q000379:methods; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism; D059808:Polyphenols / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D059808:Polyphenols
keywords: Antioxidant and binding properties; Fluorescence; Interaction of human serum albumin and polyphenols; Metabolites in kiwifruits; NMR spectroscopy