title: ABCB1 gene is associated with the risk of bullous pemphigoid in a polish population.
abstract: OBJECTIVE
  Polymorphisms in the P-glycoprotein-encoding ABCB1 gene may affect the intracellular concentration of xenobiotics, and thus contribute to the development of autoimmune diseases, including bullous pemphigoid (BP). The objective of the present study was to investigate whether there is an association between the C3435T and G2677T/A polymorphisms in the ABCB1 gene and the risk of BP in a Polish population.
  
  
  METHODS
  The study included 71 patients with BP and 156 healthy volunteers. Determination of the C3435T polymorphism was carried out using PCR-RFLP; the G2677T/A polymorphism, using allele-specific PCR.
  
  
  RESULTS
  While there was no correlation between the C3435T polymorphism and the risk of BP, we did find such an association with respect to the G2677T/A polymorphism. The relative risk of BP was more than five times greater in individuals with the 2677TA genotype (OR = 5.52, p = 0.0063), and more than twice as high in carriers of the 2677TT genotype (OR = 2.40, p = 0.0076). At 2.40 (0.000018), the OR in carriers of the 2677T allele was also increased. The greater prevalence of the 2677GG genotype and the 2677G allele in the control group, as well as the OR < 1.0 (0.22 and 0.33, respectively), suggest a protective role of the 2677G allele with respect to the development of BP.
  
  
  CONCLUSIONS
  The results of the present study indicate that the G2677T/A polymorphism in the ABCB1 gene may affect the risk of developing BP.
authors.affiliation: Department of Pharmacogenetics, Chair of Biopharmacy, Medical University of Lodz, Łódz´, Poland.
authors.forename: Mariola
authors.initials: M
authors.lastname: Rychlik-Sych
authors.affiliation: Department of Pharmacogenetics, Chair of Biopharmacy, Medical University of Lodz, Łódz´, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Barańska
authors.affiliation: Department of Pharmacogenetics, Chair of Biopharmacy, Medical University of Lodz, Łódz´, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Dudarewicz
authors.affiliation: Department of Pharmacogenetics, Chair of Biopharmacy, Medical University of Lodz, Łódz´, Poland.
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Skrętkowicz
authors.affiliation: Department of Dermatology and Venereology, Chair of Dermatology and Venereology, Medical University of Lodz, Łódź, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Żebrowska
authors.affiliation: Department of Pharmacogenetics, Chair of Biopharmacy, Medical University of Lodz, Łódz´, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Owczarek
authors.affiliation: Department of Dermatology and Venereology, Chair of Dermatology and Venereology, Medical University of Lodz, Łódź, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Waszczykowska
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics; D000328:Adult; D017677:Age Distribution; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D056726:Genetic Association Studies; D005819:Genetic Markers / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000453:epidemiology* / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010391:Pemphigoid, Bullous / Q000175:diagnosis / Q000453:epidemiology* / Q000235:genetics*; D011044:Poland / Q000453:epidemiology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D015995:Prevalence; D011379:Prognosis; D012307:Risk Factors; D017678:Sex Distribution; D055815:Young Adult
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D005819:Genetic Markers
keywords: 
  ABCB1 polymorphism; P-glycoprotein; bullous pemphigoid