title: Link between Affinity and Cu(II) Binding Sites to Amyloid-β Peptides Evaluated by a New Water-Soluble UV-Visible Ratiometric Dye with a Moderate Cu(II) Affinity.
abstract: Being able to easily determine the Cu(II) affinity for biomolecules of moderate affinity is important. Such biomolecules include amyloidogenic peptides, such as the well-known amyloid-β peptide involved in Alzheimer's disease. Here, we report the synthesis of a new water-soluble ratiometric Cu(II) dye with a moderate affinity (109M-1at pH 7.1) and the characterizations of the Cu(II) corresponding complex by X-ray crystallography, EPR, and XAS spectroscopic methods. UV-vis competition was performed on the Aβ peptide as well as on a wide series of modified peptides, leading to an affinity value of 1.6 × 109M-1at pH 7.1 for the Aβ peptide and to a coordination model for the Cu(II) site within the Aβ peptide that agrees with the one mostly accepted currently.
authors.affiliation: CNRS; LCC ( Laboratoire de Chimie de Coordination ); 205, route de Narbonne, F-31077 Toulouse, France.|Université de Toulouse ; UPS, INPT ; LCC; F-31077 Toulouse, France.
authors.forename: Amandine
authors.initials: A
authors.lastname: Conte-Daban
authors.affiliation: CNRS; LCC ( Laboratoire de Chimie de Coordination ); 205, route de Narbonne, F-31077 Toulouse, France.|Université de Toulouse ; UPS, INPT ; LCC; F-31077 Toulouse, France.
authors.forename: Valentina
authors.initials: V
authors.lastname: Borghesani
authors.affiliation: Université de Reims Champagne Ardenne , Institut de Chimie Moléculaire de Reims (ICMR), UMR 7312 CNRS-URCA, Moulin de la Housse, BP 1039, 51687 Reims Cedex 2, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Sayen
authors.affiliation: Université de Reims Champagne Ardenne , Institut de Chimie Moléculaire de Reims (ICMR), UMR 7312 CNRS-URCA, Moulin de la Housse, BP 1039, 51687 Reims Cedex 2, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Guillon
authors.affiliation: Sorbonne Universités , UPMC Univ. Paris 06, UMR 8232, IPCM, F-75005 Paris, France.|CNRS, UMR 8232, Institut Parisien de Chimie Moléculaire , F-75005 Paris, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Journaux
authors.affiliation: Sorbonne Universités , UPMC Univ. Paris 06, UMR 8232, IPCM, F-75005 Paris, France.|CNRS, UMR 8232, Institut Parisien de Chimie Moléculaire , F-75005 Paris, France.
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Gontard
authors.affiliation: Sorbonne Universités , UPMC Univ. Paris 06, UMR 8232, IPCM, F-75005 Paris, France.|CNRS, UMR 8232, Institut Parisien de Chimie Moléculaire , F-75005 Paris, France.
authors.forename: Laurent
authors.identifier: 0000-0001-8649-9022
authors.initials: L
authors.lastname: Lisnard
authors.affiliation: CNRS; LCC ( Laboratoire de Chimie de Coordination ); 205, route de Narbonne, F-31077 Toulouse, France.|Université de Toulouse ; UPS, INPT ; LCC; F-31077 Toulouse, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Hureau
mesh_terms: D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D004396:Coloring Agents / Q000737:chemistry*; D003300:Copper / Q000378:metabolism*; D018360:Crystallography, X-Ray; D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051379:Mice; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D014867:Water
chemical_list: D016229:Amyloid beta-Peptides; D004396:Coloring Agents; D014867:Water; D003300:Copper