title: A hyperbranched β-d-glucan with compact coil conformation from Lignosus rhinocerotis sclerotia.
abstract: An alkali-soluble polysaccharide was extracted from Lignosus rhinocerotis sclerotia (LRP). Its structural characteristics were determined by GC-MS, FT-IR, GC, 1D and 2D NMR combined with Smith degradation and methylation analysis. The LRP had a (1→3)-β-d-Glcp backbone with every three residues bearing a (1→6)-linked and hyperbranched side chain that contained three (1→6)-β-d-Glcp residues as secondary main chain and two terminal β-d-Glcp residues linked at O3. The degree of branching was 0.76 from GC-MS analysis, implying a highly branched structure for LRP. The Mw, <S2>z1/2, Rhand [η] values of LRP in 0.25M LiCl/DMSO were measured by SEC-MALLS-Vis-RI combination technology to be 2.88×105g/mol, 30.36nm, 22.34nm and 131.50ml/g, respectively. Furthermore, the exponent α of [η]-Mw, β of <S2>z1/2-Mw, the fractal dimension dfand molecular parameter ρ were determined to be 0.20, 0.33, 2.50 and 1.36, demonstrating that the LRP was a hyperbranched polysaccharide and adopted a compact coil conformation in LiCl/DMSO.
authors.affiliation: College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Hu
authors.affiliation: College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: hql@mail.hzau.edu.cn.
authors.forename: Qilin
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.
authors.forename: Kahing
authors.initials: K
authors.lastname: Wong
authors.affiliation: College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.affiliation: College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Jingsi
authors.initials: J
authors.lastname: Gan
authors.affiliation: College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Yanru
authors.initials: Y
authors.lastname: Li
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D005936:Glucans / Q000737:chemistry*; D008968:Molecular Conformation; D011134:Polysaccharides / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D005936:Glucans; D011134:Polysaccharides
keywords: Acetic anhydride (PubChem CID: 7918); Chain conformation; Compact coil; DMSO-d(6) (PubChem CID: 75151); Dimethyl sulfoxide (PubChem CID: 679); Ethylene glycol (PubChem CID: 174); Fractal dimension; Hydroxylamine hydrochloride (PubChem CID: 443297); Hyperbranched polysaccharide; Lignosus rhinocerotis; Methyl iodide (PubChem CID: 6328); Pyridine (PubChem CID: 1049); Sodium hydroxide (PubChem CID: 14798); Sodium periodate (PubChem CID: 23667635); Trifluoroacetic acid (PubChem CID: 6422); d-arabinose (PubChem CID: 229); d-galactose (PubChem CID: 6036); d-glucose (PubChem CID: 5793); d-mannose (PubChem CID: 18950); d-rhamnose (PubChem CID: 5460029); d-xylose (PubChem CID: 135191); β-d-glucan