title: A novel assembly pheromone trap for tick control in dog kennels.
abstract: A novel ecofriendly sticky tick trap device for the control of dog tick Rhipicephalus sanguineus using gold nanoparticle assembly pheromone complex as a bait was developed. Assembly pheromones comprising of guanine, xanthine and adenine in the ratio of 25:1:1 was encapsulated in gold nanoparticle. The response of the different stages of unfed R. sanguineus ticks was evaluated using petridish bioassay. Statistical analysis was done using chi-square test. Petridish bioassay with unfed stages of R. sanguineus revealed that 100% of the larvae, nymph and adults were attracted to assembly pheromone nanogold complex within 24h. Of the 952 ticks trapped, ticks of different stages trapped in total by the baited sticky trap device, 543 (57%) were engorged and 409 (43%) were unfed ticks. The study revealed that assembly pheromone baited traps has the potential to control tick infestations in dog kennels.
authors.affiliation: Department of Veterinary Parasitology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai-7, India. Electronic address: anishvijay145@gmail.com.
authors.forename: R K
authors.initials: RK
authors.lastname: Anish
authors.affiliation: Department of Veterinary Parasitology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai-7, India.
authors.forename: Bhaskaran Ravi
authors.initials: BR
authors.lastname: Latha
authors.affiliation: Bioproducts Lab, CSIR-Central Leather Research Institute, Chennai-20, India.
authors.forename: Giriprasath
authors.initials: G
authors.lastname: Ramanathan
authors.affiliation: Bioproducts Lab, CSIR-Central Leather Research Institute, Chennai-20, India.
authors.forename: Uma Tirichurapalli
authors.initials: UT
authors.lastname: Sivagnanam
authors.affiliation: Post Graduate Research Institute in Animal Sciences, Veterinary and Animal Sciences University, Kattupakkam, Kanchipuram 603 203, Tamil Nadu, India.
authors.forename: C
authors.initials: C
authors.lastname: Sreekumar
authors.affiliation: Bioproducts Lab, CSIR-Central Leather Research Institute, Chennai-20, India.
authors.forename: V
authors.initials: V
authors.lastname: Leela
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000469:parasitology / Q000517:prevention & control*; D004285:Dogs; D006046:Gold; D007814:Larva; D053768:Metal Nanoparticles / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission / Q000662:veterinary; D009758:Nymph; D010675:Pheromones / Q000494:pharmacology*; D048508:Rhipicephalus sanguineus / Q000187:drug effects* / Q000502:physiology; D013983:Tick Control / Q000379:methods*; D013984:Tick Infestations / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*
chemical_list: D010675:Pheromones; D006046:Gold
keywords: Assembly pheromone; Petridish bioassay; Rhipicephalus sanguineus; Sticky tick trap device