title: Magnetic sorting of membrane associated IgG for phenotype-based selection of stable antibody producing cells.
abstract: To establish a simple and widely accessible technique for rapidly selecting high producing Chinese hamster ovary (CHO) cells engineered to express a monoclonal antibody (mAb), we have exploited the transient display of recombinant protein on their cell surface. In combination with magnetic bead-based methods, we demonstrate the ability to select for cells of high productivity in the absence of any metabolic-based selection method. This technique is sufficient to obtain genetically stable engineered CHO cells via a single step of cell subcloning and yields sought-after stable, high IgG producing clonal cell lines. This technique may also be applied to other types of cells as well as polyclonal Ab cell pools.
authors.affiliation: Novimmune SA, 14 chemin des Aulx 1228 Plan-Les-Ouates, Switzerland. Electronic address: ypoitevin@novimmune.com.
authors.forename: Y
authors.initials: Y
authors.lastname: Poitevin
authors.affiliation: Novimmune SA, 14 chemin des Aulx 1228 Plan-Les-Ouates, Switzerland.
authors.forename: G
authors.initials: G
authors.lastname: Pontini
authors.affiliation: Novimmune SA, 14 chemin des Aulx 1228 Plan-Les-Ouates, Switzerland.
authors.forename: N
authors.initials: N
authors.lastname: Fischer
authors.affiliation: Novimmune SA, 14 chemin des Aulx 1228 Plan-Les-Ouates, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Kosco-Vilbois
authors.affiliation: Novimmune SA, 14 chemin des Aulx 1228 Plan-Les-Ouates, Switzerland.
authors.forename: G
authors.initials: G
authors.lastname: Elson
mesh_terms: D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology / Q000378:metabolism*; D016466:CHO Cells; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism*; D003001:Cloning, Molecular / Q000379:methods*; D003412:Cricetulus; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D007146:Immunoglobulin lambda-Chains / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D018189:Immunomagnetic Separation / Q000379:methods*; D010641:Phenotype; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D014162:Transfection
chemical_list: D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007146:Immunoglobulin lambda-Chains; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins
keywords: Cell line stability; Magnetic cell sorting; Metabolic selection free expression; Recombinant antibody production