title: How Actuated Particles Effectively Capture Biomolecular Targets.
abstract: Because of their high surface-to-volume ratio and adaptable surface functionalization, particles are widely used in bioanalytical methods to capture molecular targets. In this article, a comprehensive study is reported of the effectiveness of protein capture by actuated magnetic particles. Association rate constants are quantified in experiments as well as in Brownian dynamics simulations for different particle actuation configurations. The data reveal how the association rate depends on the particle velocity, particle density, and particle assembly characteristics. Interestingly, single particles appear to exhibit target depletion zones near their surface, caused by the high density of capture molecules. The depletion effects are even more limiting in cases with high particle densities. The depletion effects are overcome and protein capture rates are enhanced by applying dynamic particle actuation, resulting in an increase in the association rate constants by up to 2 orders of magnitude.
authors.affiliation: Department of Applied Physics, ‡Institute for Complex Molecular Systems, §Department of Biomedical Engineering, Eindhoven University of Technology , 5600 MB Eindhoven, The Netherlands.
authors.forename: Alexander
authors.initials: A
authors.lastname: van Reenen
authors.affiliation: Department of Applied Physics, ‡Institute for Complex Molecular Systems, §Department of Biomedical Engineering, Eindhoven University of Technology , 5600 MB Eindhoven, The Netherlands.
authors.forename: Arthur M
authors.identifier: 0000-0001-6019-7333
authors.initials: AM
authors.lastname: de Jong
authors.affiliation: Department of Applied Physics, ‡Institute for Complex Molecular Systems, §Department of Biomedical Engineering, Eindhoven University of Technology , 5600 MB Eindhoven, The Netherlands.
authors.forename: Menno W J
authors.identifier: 0000-0002-9788-7298
authors.initials: MW
authors.lastname: Prins
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D060328:Magnetic Phenomena; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D056004:Molecular Dynamics Simulation; D010316:Particle Size; D013499:Surface Properties
chemical_list: D000906:Antibodies; D058185:Magnetite Nanoparticles