title: Twisting electrospun nanofiber fine strips into functional sutures for sustained co-delivery of gentamicin and silver.
abstract: Surgical site infections (SSIs) represent the most common nosocomial infection among surgical patients. In order to prevent SSIs in a sustained manner and lessen side effects, we developed a twisting method for generation of nanofiber-based sutures capable of simultaneous delivery of silver and gentamicin. The prepared sutures are composed of core-sheath nanofibers with gentamicin/pluronic F127 in the core and silver/PCL in the sheath produced by co-axial electrospinning. The diameters of obtained sutures range from ~80 μm to ~1.2 mm. The in vitro release profiles of silver and gentamicin exhibit an initial burst followed by a sustained release over 5 weeks. The co-encapsulated sutures were able to kill bacteria much more effectively than gentamicin or silver alone loaded nanofiber sutures, without showing obvious impact on proliferation and migration of dermal fibroblasts and keratinocytes. The gentamicin and silver co-loaded PCL nanofiber sutures may hold great potential for prevention of SSIs.
authors.affiliation: Department of Surgery-Transplant and Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE, United States.
authors.forename: Shixuan
authors.initials: S
authors.lastname: Chen
authors.affiliation: Chongqing Academy of Animal Sciences and Key Laboratory of Pig Industry Sciences, Ministry of Agriculture, Chongqing, China.
authors.forename: Liangpeng
authors.initials: L
authors.lastname: Ge
authors.affiliation: Department of Surgery-Transplant and Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE, United States.
authors.forename: Aubrey
authors.initials: A
authors.lastname: Mueller
authors.affiliation: Department of Surgery-General Surgery, University of Nebraska Medical Center, Omaha, NE, United States.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Carlson
authors.affiliation: Department of Orthopaedic Surgery and Rehabilitation, University of Nebraska Medical Center, Omaha, NE, United States.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Teusink
authors.affiliation: Department of Orthopaedic Surgery, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV, United States.
authors.forename: Franklin D
authors.initials: FD
authors.lastname: Shuler
authors.affiliation: Department of Surgery-Transplant and Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE, United States. Electronic address: jingwei.xie@unmc.edu.
authors.forename: Jingwei
authors.initials: J
authors.lastname: Xie
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry; D002460:Cell Line; D003428:Cross Infection / Q000188:drug therapy; D016503:Drug Delivery Systems; D065546:Drug Liberation; D005839:Gentamicins / Q000737:chemistry*; D006801:Humans; D008826:Microbial Sensitivity Tests; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012834:Silver / Q000737:chemistry*; D013530:Surgical Wound Infection / Q000188:drug therapy; D013537:Sutures
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D011091:Polyesters; C016240:polycaprolactone; D012834:Silver
keywords: Anti-bacterial; Co-delivery; Electrospun nanofibers; Surgical site infections; Suture