title: Analysis of serotonin concentrations in human milk by high-performance liquid chromatography with fluorescence detection.
abstract: Serotonin (5-hydroxytryptamine, 5-HT) plays an important role in milk volume homeostasis in the mammary gland during lactation; 5-HT in milk may also affect infant development. However, there are few reports on 5-HT concentrations in human breast milk. To address this issue, we developed a simple method based on high-performance liquid chromatography with fluorescence detection (HPLC-FD) for measuring 5-HT concentrations in human breast milk. Breast milk samples were provided by four healthy Japanese women. Calibration curves for 5-HT in each sample were prepared with the standard addition method between 5 and 1000 ng/ml, and all had correlation coefficients >0.999. The recovery of 5-HT was 96.1%-101.0%, with a coefficient of variation of 3.39%-8.62%. The range of 5-HT concentrations estimated from the calibration curves was 11.1-51.1 ng/ml. Thus, the HPLC-FD method described here can effectively extract 5-HT from human breast milk with high reproducibility.
authors.affiliation: Department of Clinical Pharmaceutics and Pharmacy Practice, School of Pharmacy, Iwate Medical University, 2-1-1, Nishitokuta, Yahaba-cho, Shiwa-gun, Iwate 028-3603, Japan. Electronic address: chibat@iwate-med.ac.jp.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Chiba
authors.affiliation: Department of Pharmacology, Nihon Pharmaceutical University, 10281 Komuro, Ina-machi, Kitaadachi-gun, Saitama 362-0862, Japan. Electronic address: t-maeda@nichiyaku.ac.jp.
authors.forename: Tomoji
authors.initials: T
authors.lastname: Maeda
authors.affiliation: Department of Clinical Pharmaceutics and Pharmacy Practice, School of Pharmacy, Iwate Medical University, 2-1-1, Nishitokuta, Yahaba-cho, Shiwa-gun, Iwate 028-3603, Japan. Electronic address: tomohiko.tairabune@j.iwate-med.ac.jp.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Tairabune
authors.affiliation: Department of Clinical Pharmaceutics and Pharmacy Practice, School of Pharmacy, Iwate Medical University, 2-1-1, Nishitokuta, Yahaba-cho, Shiwa-gun, Iwate 028-3603, Japan. Electronic address: tatomita@iwate-med.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Tomita
authors.affiliation: Department of Pharmacotherapeutics, School of Pharmacy, Iwate Medical University, 2-1-1, Nishitokuta, Yahaba-cho, Shiwa-gun, Iwate 028-3603, Japan. Electronic address: asanbe@iwate-med.ac.jp.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Sanbe
authors.affiliation: Department of Nursing, Iwate Medical University Hospital, 19-1, Uchimaru, Morioka, Iwate 020-8505, Japan. Electronic address: rika.takeda@j.iwate-med.ac.jp.
authors.forename: Rika
authors.initials: R
authors.lastname: Takeda
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Iwate Medical University, 19-1, Uchimaru, Morioka, Iwate 020-8505, Japan. Electronic address: akikuchi@iwate-med.ac.jp.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kikuchi
authors.affiliation: Department of Clinical Pharmaceutics and Pharmacy Practice, School of Pharmacy, Iwate Medical University, 2-1-1, Nishitokuta, Yahaba-cho, Shiwa-gun, Iwate 028-3603, Japan. Electronic address: kenzkudo@iwate-med.ac.jp.
authors.forename: Kenzo
authors.initials: K
authors.lastname: Kudo
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods*; D005260:Female; D005453:Fluorescence; D006801:Humans; D007774:Lactation; D008895:Milk, Human / Q000737:chemistry*; D012701:Serotonin / Q000032:analysis*
chemical_list: D012701:Serotonin
keywords: Fluorescence detection; High performance liquid chromatography; Human breast milk; Serotonin