title: High throughput discovery of new fouling-resistant surfaces.
abstract: A novel high throughput method for synthesis and screening of customized protein-resistant surfaces was developed. This method is an inexpensive, fast, reproducible and scalable approach to synthesize and screen protein-resistant surfaces appropriate for a specific feed. The method is illustrated here by combining a high throughput platform (HTP) approach together with our patented photo-induced graft polymerization (PGP) method developed for facile modification of commercial poly(aryl sulfone) membranes. We demonstrate that the HTP-PGP approach to synthesize and screen fouling-resistant surfaces is general, and thus provides the capability to develop surfaces optimized for specific feeds. Surfaces were preparedviagraft polymerization onto poly(ether sulfone) (PES) membranes and were evaluated using a protein adsorption assay followed by pressure-driven filtration. We have employed the HTP-PGP approach to confirm previously reported successful monomers and to develop new antifouling surfaces from a library of 66 monomers for four different challenges of interest to the biotechnology community: hen egg-white lysozyme, supernatant from Chinese Hamster Ovary (CHO) cells in phosphate buffered saline (PBS) solution as a model cell suspension, and immunoglobulin G (IgG) precipitated in the absence and presence of bovine serum albumin (BSA) in high salt solution as a model precipitation process.
authors.affiliation: Department of Civil and Environmental Engineering, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.
authors.forename: Mingyan
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Howard P. Isermann Department of Chemical and Biological Engineering, and the Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Liu
authors.affiliation: Howard P. Isermann Department of Chemical and Biological Engineering, and the Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.
authors.forename: Adith
authors.initials: A
authors.lastname: Venkiteshwaran
authors.affiliation: Department of Civil and Environmental Engineering, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.
authors.forename: James
authors.initials: J
authors.lastname: Kilduff
authors.affiliation: Department of Chemical Engineering, Harvard-MIT Division of Health Sciences & Technology, David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA.
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Anderson
authors.affiliation: Department of Chemical Engineering, Harvard-MIT Division of Health Sciences & Technology, David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Langer
authors.affiliation: Howard P. Isermann Department of Chemical and Biological Engineering, and the Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.
authors.forename: Georges
authors.initials: G
authors.lastname: Belfort