title: MiRNA-Sequence Indicates That Mesenchymal Stem Cells and Exosomes Have Similar Mechanism to Enhance Cardiac Repair.
abstract: Mesenchymal stem cells (MSCs) repair infarcted heart through paracrine mechanism. We sought to compare the effectiveness of MSCs and MSC-derived exosomes (MSC-Exo) in repairing infarcted hearts and to identify how MSC-Exo mediated cardiac repair is regulated. In a rat myocardial infarction model, we found that MSC-Exo inhibited cardiac fibrosis, inflammation, and improved cardiac function. The beneficial effects of MSC-Exo were significantly superior compared to that of MSCs. To explore the potential mechanisms underlying MSC-Exo's effects, we performed several in vitro experiments and miRNA-sequence analysis. MSC-Exo stimulated cardiomyocyte H9C2 cell proliferation, inhibited apoptosis induced by H2O2, and inhibited TGF-βinduced transformation of fibroblast cell into myofibroblast. Importantly, novel miRNA sequencing results indicated that MSC-Exo and MSCs have similar miRNA expression profile, which could be one of the reasons that MSC-Exo can replace MSCs for cardiac repair. In addition, the expression of several miRNAs from MSC-Exo was significantly different from that of MSCs, which may explain why MSC-Exo has better therapeutic effect than MSCs. In conclusion, this study demonstrates that MSC-Exo could be used alone to promote cardiac repair and are superior to MSCs in repairing injured myocardium.
authors.affiliation: Department of Cardiovascular Surgery, The First Affiliated Hospital and The Institute for Cardiovascular Science, Soochow University, Suzhou, Jiangsu 215123, China.
authors.forename: Lianbo
authors.identifier: 0000-0001-9322-9581
authors.initials: L
authors.lastname: Shao
authors.affiliation: Department of Cardiovascular Surgery, The First Affiliated Hospital and The Institute for Cardiovascular Science, Soochow University, Suzhou, Jiangsu 215123, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Cardiovascular Surgery, The First Affiliated Hospital and The Institute for Cardiovascular Science, Soochow University, Suzhou, Jiangsu 215123, China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Lan
authors.affiliation: Department of Cardiovascular Surgery, The First Affiliated Hospital and The Institute for Cardiovascular Science, Soochow University, Suzhou, Jiangsu 215123, China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Cardiovascular Surgery, The First Affiliated Hospital and The Institute for Cardiovascular Science, Soochow University, Suzhou, Jiangsu 215123, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Cardiovascular Surgery, The First Affiliated Hospital and The Institute for Cardiovascular Science, Soochow University, Suzhou, Jiangsu 215123, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Cardiovascular Surgery, The First Affiliated Hospital and The Institute for Cardiovascular Science, Soochow University, Suzhou, Jiangsu 215123, China.
authors.forename: Pengli
authors.initials: P
authors.lastname: Xiao
authors.affiliation: Department of Cardiovascular Surgery, The First Affiliated Hospital and The Institute for Cardiovascular Science, Soochow University, Suzhou, Jiangsu 215123, China.
authors.forename: Qingyou
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Department of Internal Medicine, University of Texas, Houston, TX 77583, USA.
authors.forename: Yong-Jian
authors.initials: YJ
authors.lastname: Geng
authors.affiliation: Guangzhou Medical University, Guangzhou, Guangdong 510080, China.
authors.forename: Xi-Yong
authors.initials: XY
authors.lastname: Yu
authors.affiliation: Department of Cardiovascular Surgery, The First Affiliated Hospital and The Institute for Cardiovascular Science, Soochow University, Suzhou, Jiangsu 215123, China.
authors.forename: Yangxin
authors.identifier: 0000-0002-2364-3207
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D055354:Exosomes / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009203:Myocardial Infarction / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D032383:Myocytes, Cardiac / Q000378:metabolism; D018919:Neovascularization, Physiologic / Q000235:genetics; D019899:Paracrine Communication / Q000235:genetics; D051381:Rats; D012038:Regeneration / Q000235:genetics*
chemical_list: D035683:MicroRNAs