title: Rbm46 regulates mouse embryonic stem cell differentiation by targeting β-Catenin mRNA for degradation.
abstract: Embryonic stem cells (ESCs) are pluripotent cells and have the capability for differentiation into any of the three embryonic germ layers. The Wnt/β-Catenin pathway has been shown to play an essential role in ESC differentiation regulation. Activation of β-Catenin by post-translational modification has been extensively studied. However, mechanism(s) of post-transcriptional regulation of β-Catenin are not well defined. In this study, we report an RNA recognition motif-containing protein (RNA binding motif protein 46, RBM46) which regulates the degradation of β-Catenin mRNA. Our results show that Rbm46 is distributed primarily in the cytoplasm of mouse ESCs (mESCs) and is elevated during the process of ESC differentiation. In addition, overexpression of Rbm46 results in differentiation of mESCs into trophectoderm, while knock-down of Rbm46 leads to mESC differentiation into endoderm. β-Catenin, a key effector in the Wnt pathway which has been reported to play a significant role in the regulation of ESC differentiation, is post-transcriptionally regulated by Rbm46. Our study reveals Rbm46 plays a novel role in the regulation of ESC differentiation.
authors.affiliation: Department of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.|Peking University Stem Cell Research Center, China National Center for International Research, Peking University Health Science Center, Beijing, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhai
authors.affiliation: Department of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.|Peking University Stem Cell Research Center, China National Center for International Research, Peking University Health Science Center, Beijing, China.
authors.forename: Chenchen
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.|Peking University Stem Cell Research Center, China National Center for International Research, Peking University Health Science Center, Beijing, China.
authors.forename: Yuanfan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.|Peking University Stem Cell Research Center, China National Center for International Research, Peking University Health Science Center, Beijing, China.
authors.forename: Shixin
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.|Peking University Stem Cell Research Center, China National Center for International Research, Peking University Health Science Center, Beijing, China.|SARI Center for Stem Cell and Nanomedicine, Shanghai Advanced Research Institute, University of Chinese Academy of Sciences, Shanghai, China.
authors.forename: Lingsong
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D002478:Cells, Cultured; D004707:Endoderm / Q000166:cytology / Q000502:physiology; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D051379:Mice; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology; D020871:RNA Stability; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D051176:beta Catenin / Q000235:genetics* / Q000378:metabolism
chemical_list: C495267:CTNNB1 protein, mouse; C000604616:Rbm46 protein, mouse; D016601:RNA-Binding Proteins; D051176:beta Catenin