title: Impairment of infectious laryngotracheitis virus replication by deletion of the UL[-1] gene.
abstract: Infectious laryngotracheitis virus (ILTV) encodes several unique genes, including a pair of unique nuclear proteins UL0 and UL[-1] that are expressed during replication in cell culture. Although the UL0 gene has been shown to be dispensable for replication, the role of UL[-1] has not been elucidated. In this study a deletion mutant of ILTV lacking the UL[-1] gene was constructed using homologous recombination. The coding sequences of the gene were replaced with the gene for enhanced green fluorescent protein and the cytomegalovirus major immediate early promoter element. The progeny virus carrying the reporter gene was readily identified using fluorescent microscopy, but was unable to propagate in the permissive cells in the absence of wild type ILTV. Even after plaque purification and fluorescent associated cell sorting the recombinant virus deficient in UL[-1] gene could not be successfully isolated. Our findings suggest that the UL[-1] gene has an important role in ILTV replication.
authors.affiliation: Centre for Equine Infectious Diseases, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, 3010, Australia.|Asia-Pacific Centre for Animal Health, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, 3010, Australia.
authors.forename: M
authors.initials: M
authors.lastname: Nadimpalli
authors.affiliation: Asia-Pacific Centre for Animal Health, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, 3010, Australia.|College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.
authors.forename: S W
authors.initials: SW
authors.lastname: Lee
authors.affiliation: Asia-Pacific Centre for Animal Health, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, 3010, Australia.
authors.forename: J M
authors.initials: JM
authors.lastname: Devlin
authors.affiliation: Centre for Equine Infectious Diseases, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, 3010, Australia.
authors.forename: J R
authors.initials: JR
authors.lastname: Gilkerson
authors.affiliation: Centre for Equine Infectious Diseases, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, 3010, Australia. carolah@unimelb.edu.au.|Asia-Pacific Centre for Animal Health, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, 3010, Australia. carolah@unimelb.edu.au.
authors.forename: C A
authors.initials: CA
authors.lastname: Hartley
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002645:Chickens / Q000821:virology; D004261:DNA Replication; D017353:Gene Deletion; D017930:Genes, Reporter; D005814:Genes, Viral; D016679:Genome, Viral; D049452:Green Fluorescent Proteins / Q000235:genetics; D001352:Herpesvirus 1, Gallid / Q000235:genetics* / Q000502:physiology*; D059765:Homologous Recombination; D017384:Sequence Deletion; D014764:Viral Proteins / Q000235:genetics* / Q000502:physiology; D014779:Virus Replication / Q000235:genetics*
chemical_list: D014764:Viral Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins