title: Exogenous enzyme complex prevents intestinal soybean meal-induced enteritis in Mugil liza (Valenciennes, 1836) juvenile.
abstract: Four soybean meal-based diets containing increasing levels of an enzyme complex (E50, E100, E150 and E200 at 50, 100, 150 and 200 g ton-1, respectively) and one soybean meal-based diet without the enzyme complex (E0) were fed in triplicate to M. liza juveniles in a semi-static flow system with 20 fish per tank for 75 days. There were no differences between the treatments for animal performance parameters, but fish fed the enzyme complex treatment exhibited significantly (P<0.05) higher values of calcium bone retention compared with control fish. Although there was no relationship between bacterial counts in different sections of the gastrointestinal tract or enzyme levels, filamentous bacteria were increased in E50 compared with E150. All of the treatments resulted in higher bacterial counts in the stomach than in intestinal segments. Histological screening showed serious to moderate infiltration of inflammatory cells, modification in villus morphology and necrosis in some cases in fish fed the E0 diet. In addition, fish from the E0 treatment exhibited significantly (P<0.05) lower lipid deposition in the peritoneal cavity. Therefore, the use of low levels of exogenous enzyme is recommended in diets for M. liza when soybean meal is used as the main source of protein.
authors.affiliation: Programa de Pós-Graduação em Aquicultura, Universidade Federal do Rio Grande/FURG, Avenida Itália, Km 8, s/n, Bairro Carreiros, 96201-900 Rio Grande, RS, Brazil.
authors.forename: Leonardo R V
authors.initials: LRV
authors.lastname: Ramos
authors.affiliation: Programa de Pós-Graduação em Aquicultura, Universidade Federal do Rio Grande/FURG, Avenida Itália, Km 8, s/n, Bairro Carreiros, 96201-900 Rio Grande, RS, Brazil.
authors.forename: Virgínia F
authors.initials: VF
authors.lastname: Pedrosa
authors.affiliation: Adisseo Brasil Nutrição Animal, Avenida Maria Coelho Aguiar, 215, Bloco G, 1º andar, Jardim São Luis, 05804-900 São Paulo, SP, Brazil.
authors.forename: Agnes
authors.initials: A
authors.lastname: Mori
authors.affiliation: Programa de Pós-Graduação em Aquicultura, Universidade Federal do Rio Grande/FURG, Avenida Itália, Km 8, s/n, Bairro Carreiros, 96201-900 Rio Grande, RS, Brazil.
authors.forename: Carlos F F DE
authors.initials: CFF
authors.lastname: Andrade
authors.affiliation: Programa de Pós-Graduação em Aquicultura, Universidade Federal do Rio Grande/FURG, Avenida Itália, Km 8, s/n, Bairro Carreiros, 96201-900 Rio Grande, RS, Brazil.
authors.forename: Luis A
authors.initials: LA
authors.lastname: Romano
authors.affiliation: Programa de Pós-Graduação em Aquicultura, Universidade Federal do Rio Grande/FURG, Avenida Itália, Km 8, s/n, Bairro Carreiros, 96201-900 Rio Grande, RS, Brazil.
authors.forename: Paulo C
authors.initials: PC
authors.lastname: Abreu
authors.affiliation: Programa de Pós-Graduação em Aquicultura, Universidade Federal do Rio Grande/FURG, Avenida Itália, Km 8, s/n, Bairro Carreiros, 96201-900 Rio Grande, RS, Brazil.
authors.forename: Marcelo B
authors.initials: MB
authors.lastname: Tesser
mesh_terms: D000704:Analysis of Variance; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D058491:Bacterial Load; D004032:Diet / Q000662:veterinary*; D004751:Enteritis / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary*; D004798:Enzymes / Q000008:administration & dosage*; D005393:Fish Diseases / Q000517:prevention & control*; D041981:Gastrointestinal Tract / Q000382:microbiology / Q000473:pathology; D012016:Reference Values; D015203:Reproducibility of Results; D023701:Smegmamorpha / Q000254:growth & development* / Q000382:microbiology; D013025:Glycine max; D018709:Statistics, Nonparametric; D013997:Time Factors
chemical_list: D004798:Enzymes