title: A new dermocosmetic containing retinaldehyde, delta-tocopherol glucoside and glycylglycine oleamide for managing naturally aged skin: results from in vitro to clinical studies.
abstract: BACKGROUND
  Natural aging of skin tissues, the addition of the cumulative action of the time and radiation exposure result in skin atrophy, wrinkles and degeneration of the extracellular matrix (ECM). The aim of the study was to investigate the beneficial effect of a combination containing retinaldehyde (RAL), delta-tocopherol glucoside (delta-TC) and glycylglycine ole-amide (GGO) and of a dermocosmetic containing the combination.
  
  
  METHODS
  The protective effect of the combination was assessed through in vitro gene expression of ultraviolet (UV)-irradiated fibroblasts. A skin aging assay using UV light on ex vivo skin samples and a clinical study conducted in 36 women aged from 35 to 55 years with a minimum of level 4 to a maximum of level 6 on the crow's feet photoscale assessed the antiaging effect of the dermocosmetic.
  
  
  RESULTS
  When added to UV-irradiated fibroblasts, the combination substantially improved the ECM in activating the elastin fiber production (fibrillin 2,fibulin 1and5andlysyl oxidase-like 2) as well as that of proteins involved in the cellular ECM interactions (integrin b1,paxillinandactin a2). An ex vivo photodamaged human skin model showed that the dermocosmetic formulation containing the combination of the active ingredients protected the elastic network against UV-induced alterations including both elastin and fibrillin-rich fibers in the dermis. A daily application of the dermocosmetic for 2 months on naturally aged skin resulted in a statistically significant improvement (p<0.05) of visible signs of aging comprising crow's feet, wrinkles and periocular fine lines. Finally, the formulation was well tolerated.
  
  
  CONCLUSIONS
  The dermocosmetic containing RAL, delta-TC and GGO provides a substantial benefit in the daily care of naturally aged skin in women aged 35-55 years.
authors.affiliation: Clinical Skin Research Center.
authors.forename: Céline
authors.initials: C
authors.lastname: Rouvrais
authors.affiliation: Department of Pharmacology, Pierre Fabre Dermo-Cosmétique, Toulouse.
authors.forename: Daniel
authors.initials: D
authors.lastname: Bacqueville
authors.affiliation: Department of Pharmacology, Pierre Fabre Dermo-Cosmétique, Toulouse.
authors.forename: Patrick
authors.initials: P
authors.lastname: Bogdanowicz
authors.affiliation: Department of Pharmacology, Pierre Fabre Dermo-Cosmétique, Toulouse.
authors.forename: Marie-José
authors.initials: MJ
authors.lastname: Haure
authors.affiliation: Department of Pharmacology, Pierre Fabre Dermo-Cosmétique, Toulouse.
authors.forename: Laure
authors.initials: L
authors.lastname: Duprat
authors.affiliation: Laboratoire Dermatologique Avène, Lavaur, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Coutanceau
authors.affiliation: Department of Pharmacology, Pierre Fabre Dermo-Cosmétique, Toulouse.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Castex-Rizzi
authors.affiliation: Department of Pharmacology, Pierre Fabre Dermo-Cosmétique, Toulouse.
authors.forename: Hélène
authors.initials: H
authors.lastname: Duplan
authors.affiliation: Clinical Skin Research Center.
authors.forename: Valérie
authors.initials: V
authors.lastname: Mengeaud
authors.affiliation: Department of Pharmacology, Pierre Fabre Dermo-Cosmétique, Toulouse.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Bessou-Touya
keywords: delta-tocopherol glucoside; formulation; glycylglycine oleamide; preclinical aged skin model; retinaldehyde; statistics