title: Expression and distribution of extensins and AGPs in susceptible and resistant banana cultivars in response to wounding and Fusarium oxysporum.
abstract: Banana Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc) is soil-borne disease of banana (Musa spp.) causing significant economic losses. Extensins and arabinogalactan proteins (AGPs) are cell wall components important for pathogen defence. Their significance for Foc resistance in banana was not reported so far. In this study, two banana cultivars differing in Foc sensitivity were used to monitor the changes in transcript levels, abundance and distribution of extensins and AGPs after wounding and Foc inoculation. Extensins mainly appeared in the root cap and meristematic cells. AGPs recognized by JIM13, JIM8, PN16.4B4 and CCRC-M134 antibodies located in root hairs, xylem and root cap. Individual AGPs and extensins showed specific radial distribution in banana roots. At the transcript level, seven extensins and 23 AGPs were differentially expressed between two banana cultivars before and after treatments. Two extensins and five AGPs responded to the treatments at the protein level. Most extensins and AGPs were up-regulated by wounding and pathogen inoculation of intact plants but down-regulated by pathogen attack of wounded plants. Main components responsible for the resistance of banana were MaELP-2 and MaPELP-2. Our data revealed that AGPs and extensins represent dynamic cell wall components involved in wounding and Foc resistance.
authors.affiliation: College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Yunli
authors.initials: Y
authors.lastname: Wu
authors.affiliation: College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Fan
authors.affiliation: Institute of Biotechnology, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
authors.forename: Xiaoquan
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Houbin
authors.initials: H
authors.lastname: Chen
authors.affiliation: Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, 783 71 Olomouc, Czech Republic.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Takáč
authors.affiliation: Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, 783 71 Olomouc, Czech Republic.
authors.forename: Olga
authors.initials: O
authors.lastname: Šamajová
authors.affiliation: College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Musana Rwalinda
authors.initials: MR
authors.lastname: Fabrice
authors.affiliation: College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Xie
authors.affiliation: College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Ma
authors.affiliation: Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, 783 71 Olomouc, Czech Republic.
authors.forename: Jozef
authors.initials: J
authors.lastname: Šamaj
authors.affiliation: College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Chunxiang
authors.initials: C
authors.lastname: Xu
mesh_terms: D060467:Disease Resistance / Q000235:genetics*; D000939:Epitopes / Q000276:immunology; D005670:Fusarium; D018506:Gene Expression Regulation, Plant; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D054884:Host-Pathogen Interactions / Q000235:genetics; D009088:Mucoproteins / Q000235:genetics* / Q000378:metabolism; D028521:Musa / Q000382:microbiology* / Q000502:physiology*; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D010940:Plant Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D021381:Protein Transport
chemical_list: D000939:Epitopes; D006023:Glycoproteins; D009088:Mucoproteins; D010940:Plant Proteins; C006619:arabinogalactan proteins; C005488:extensin protein, plant