title: Effects of selenium-lead interaction on the gene expression of inflammatory factors and selenoproteins in chicken neutrophils.
abstract: Lead (Pb) is one of the most highly toxic metal pollutant that can cause damage to the immune system. It is known that selenium (Se) can antagonize heavy metals. To explore the toxic effects of Pb poisoning on bird immune cells, as well as the alleviating effects of Se on Pb, Se supplement and/or Pb poisoning chicken models were established. One hundred and eighty Hyline 7-day-old male chickens received either Se (1mg Se per kg of diet), Pb (350mg Pb per liter water) or Se+Pb in their diet and water for 90 days. Then, whole blood was collected from the four groups of chickens, and serum and neutrophils were isolated. The levels of Se and Pb in chicken serum, mRNA levels of 24 selenoproteins (GPX1, GPX2, GPX3, GPX4, Dio1, Dio2, Dio3, Txnrd1, Txnrd2, Txnrd3, SELS, SPS2, SELK, SELW1, SEP15, SEPX1, SELT, SELI, SELO, SELM, SEPN1, SEPP1, SELU, SELH) and inflammatory factors (TNF-α, COX-2, iNOS, NF-κB), and iNOS protein level in chicken neutrophils were determined, and protein-protein interaction prediction and principal component analysis were performed. The data showed that Pb exposure increased Pb content in serum, activated the NF-κB pathway, and increased the expression of selenoproteins in chicken neutrophils. Se supplements could reduce Pb concentration in serum, had a mitigative effect on the activation of the NF-κB pathway and further enhanced the upward trend of selenoprotein expression induced by Pb exposure. These results suggest that Se supplement could eliminate Pb in serum and alleviate the activation of the NF-κB pathway under Pb exposure by increasing the expression of selenoproteins.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Mengyuan
authors.initials: M
authors.lastname: Xing
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Menghao
authors.initials: M
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Jinxin
authors.initials: J
authors.lastname: Zhao
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Ruifeng
authors.initials: R
authors.lastname: Fan
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Changyu
authors.initials: C
authors.lastname: Cao
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China. Electronic address: zhangziwe@sina.com.
authors.forename: Ziwei
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China. Electronic address: shiwenxu@neau.edu.cn.
authors.forename: Shiwen
authors.initials: S
authors.lastname: Xu
mesh_terms: D000818:Animals; D002645:Chickens / Q000097:blood / Q000276:immunology; D004347:Drug Interactions; D015870:Gene Expression / Q000187:drug effects; D018836:Inflammation Mediators / Q000097:blood*; D007854:Lead / Q000097:blood / Q000494:pharmacology*; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012643:Selenium / Q000494:pharmacology*; D051140:Selenoproteins / Q000097:blood* / Q000235:genetics
chemical_list: D018836:Inflammation Mediators; D016328:NF-kappa B; D012333:RNA, Messenger; D051140:Selenoproteins; D007854:Lead; D012643:Selenium
keywords: Chicken; Inflammatory factor; Lead; Neutrophil; Selenium; Selenoprotein