title: Repair of bone defects using adipose-derived stem cells combined with alpha-tricalcium phosphate and gelatin sponge scaffolds in a rat model.
abstract: OBJECTIVE
  This study aimed to evaluate the potential of adipose-derived stem cells (ASCs) combined with a modified α-tricalcium phosphate (α-TCP) or gelatin sponge (GS) scaffolds for bone healing in a rat model.
  
  
  METHODS
  Bone defects were surgically created in the femur of adult SHR rats and filled with the scaffolds, empty or combined with ASCs. The results were analyzed by histology and histomorphometry on days seven, 14, 30, and 60.
  
  
  RESULTS
  Significantly increased bone repair was observed on days seven and 60 in animals treated with α-TCP/ASCs, and on day 14 in the group treated with GS/ASCs, when compared with the groups treated with the biomaterials alone. Intense fibroplasia was observed in the group treated with GS alone, on days 14 and 30.
  
  
  CONCLUSIONS
  Our results showed that the use of ASCs combined with α-TCP or GS scaffolds resulted in increased bone repair. The higher efficacy of the α-TCP scaffold suggests osteoconductive property that results in a biological support to the cells, whereas the GS scaffold functions just as a carrier. These results confirm the potential of ASCs in accelerating bone repair in in vivo experimental rat models. These results suggest a new alternative for treating bone defects.
authors.affiliation: Universidade Federal do Rio Grande do Sul, Faculdade de Odontologia, Porto Alegre, RS, Brasil.
authors.forename: Adriana
authors.initials: A
authors.lastname: Corsetti
authors.affiliation: Irmandade da Santa Casa de Misericórdia de Porto Alegre, Centro de Odontologia, Cirurgia e Reabilitação Bucomaxilofacial, Porto Alegre, RS, Brasil, Universidade Luterana do Brasil, Laboratório de Células-Tronco e Engenharia de Tecidos, Canoas, RS, Brasil.
authors.forename: Claudia
authors.initials: C
authors.lastname: Bahuschewskyj
authors.affiliation: Universidade Federal do Rio Grande do Sul, Faculdade de Odontologia, Porto Alegre, RS, Brasil.
authors.forename: Deise
authors.initials: D
authors.lastname: Ponzoni
authors.affiliation: Universidade Federal do Rio Grande do Sul, Faculdade de Odontologia, Porto Alegre, RS, Brasil.
authors.forename: Renan
authors.initials: R
authors.lastname: Langie
authors.affiliation: Universidade Federal do Rio Grande do Sul, Departamento de Engenharia de Materiais, Porto Alegre, RS, Brasil.
authors.forename: Luis Alberto Dos
authors.initials: LA
authors.lastname: Santos
authors.affiliation: Universidade Luterana do Brasil, Laboratório de Células-Tronco e Engenharia de Tecidos, Canoas, RS, Brasil.
authors.forename: Melissa
authors.initials: M
authors.lastname: Camassola
authors.affiliation: Universidade Luterana do Brasil, Laboratório de Células-Tronco e Engenharia de Tecidos, Canoas, RS, Brasil.
authors.forename: Nance Beyer
authors.initials: NB
authors.lastname: Nardi
authors.affiliation: Irmandade da Santa Casa de Misericórdia de Porto Alegre, Centro de Odontologia, Cirurgia e Reabilitação Oral e Maxilofacial, Porto Alegre, RS, Brasil.
authors.forename: Edela
authors.initials: E
authors.lastname: Puricelli
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology* / Q000627:therapeutic use; D001861:Bone Regeneration / Q000187:drug effects*; D002130:Calcium Phosphates / Q000494:pharmacology* / Q000627:therapeutic use; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005269:Femur / Q000473:pathology / Q000601:surgery; D005347:Fibroblasts / Q000187:drug effects; D005562:Formazans; D005781:Gelatin Sponge, Absorbable / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D023421:Models, Animal; D010012:Osteogenesis / Q000187:drug effects; D011918:Rats, Inbred SHR; D015203:Reproducibility of Results; D033581:Stem Cell Transplantation / Q000379:methods*; D013778:Tetrazolium Salts; D013997:Time Factors; D054457:Tissue Scaffolds; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D005562:Formazans; D013778:Tetrazolium Salts; C485828:alpha-tricalcium phosphate; C079782:MTT formazan