title: microRNA-802 inhibits epithelial-mesenchymal transition through targeting flotillin-2 in human prostate cancer.
abstract: miRNAs are a class of non-coding RNAs that exert critical roles in various biological processes. The aim of the present study was to identify the functional roles ofmiR-802in regulating epithelial-mesenchymal transition (EMT) in prostate cancer (PCa).miR-802expression was detected in 73 pairs of PCa samples and PCa cell lines (PC3 and DU145 cells) by qRT-PCR. Cell proliferation was detected using MTT assay, and cell apoptosis was evaluated using flow cytometry. Transwell assay was conducted to investigate cell migration and invasion. Expression analysis of a set of EMT markers was performed to explore whethermiR-802is involved in EMT program. Xenograft model was established to investigate the function ofmiR-802in carcinogenesisin vivoThe direct regulation of Flotillin-2 (Flot2) bymiR-802was identified using luciferase reporter assay.miR-802was remarkably down-regulated in PCa tissues and cell lines. Gain-of-function trails showed thatmiR-802serves as an 'oncosuppressor' in PCa through inhibiting cell proliferation and promoting cell apoptosisin vitroOverexpression ofmiR-802significantly suppressedin vivoPCa tumor growth. Luciferase reporter analysis identified Flot2 as a direct target ofmiR-802in PCa cells. OverexpressedmiR-802significantly suppressed EMT, migration and invasion in PCa cells by regulating Flot2. We identifiedmiR-802as a novel tumor suppressor in PCa progression and elucidated a novel mechanism of themiR-802/Flot2 axis in the regulation of EMT, which may be a potential therapeutic target.
authors.affiliation: Department of Urology, Ruijin Hospital North, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Urology, Ruijin Hospital North, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Lu
authors.affiliation: Department of Urology, Ruijin Hospital North, School of Medicine, Shanghai Jiao Tong University, Shanghai, China shaoyuan1602@163.com daxu@medmail.com.cn.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Shao
authors.affiliation: Department of Urology, Ruijin Hospital North, School of Medicine, Shanghai Jiao Tong University, Shanghai, China shaoyuan1602@163.com daxu@medmail.com.cn.
authors.forename: Da
authors.initials: D
authors.lastname: Xu
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014183:Transplantation, Heterologous
chemical_list: D020413:3' Untranslated Regions; C552280:MIRN802 microRNA, human; D008565:Membrane Proteins; D035683:MicroRNAs; C106354:flotillins
keywords: Flot2; epithelial-mesenchymal transition; metastasis; microRNA-802; prostate cancer