title: Water-Soluble Coenzyme Q10 Reduces Rotenone-Induced Mitochondrial Fission.
abstract: Parkinson's disease is a neurodegenerative disorder characterized by mitochondrial dysfunction and oxidative stress. It is usually accompanied by an imbalance in mitochondrial dynamics and changes in mitochondrial morphology that are associated with impaired function. The objectives of this study were to identify the effects of rotenone, a drug known to mimic the pathophysiology of Parkinson's disease, on mitochondrial dynamics. Additionally, this study explored the protective effects of water-soluble Coenzyme Q10 (CoQ10) against rotenone-induced cytotoxicity in murine neuronal HT22 cells. Our results demonstrate that rotenone elevates protein expression of mitochondrial fission markers, Drp1 and Fis1, and causes an increase in mitochondrial fragmentation as evidenced through mitochondrial staining and morphological analysis. Water-soluble CoQ10 prevented mitochondrial dynamic imbalance by reducing Drp1 and Fis1 protein expression to pre-rotenone levels, as well as reducing rotenone treatment-associated mitochondrial fragmentation. Hence, water-soluble CoQ10 may have therapeutic potential in treating patients with Parkinson's disease.
authors.affiliation: Department of Neurology, General Hospital of Ningxia Medical University, Ningxia Key Laboratory of Cerebrocranial Diseases, Incubation Base of National Key Laboratory, Yinchuan, 750004, China.|Department of Pharmaceutical Sciences, Biomanufacturing Research Institute Biotechnology Enterprise (BRITE), North Carolina Central University, Durham, NC, 27707, USA.
authors.forename: Hai-Ning
authors.initials: HN
authors.lastname: Li
authors.affiliation: Department of Pharmaceutical Sciences, Biomanufacturing Research Institute Biotechnology Enterprise (BRITE), North Carolina Central University, Durham, NC, 27707, USA.
authors.forename: Mary
authors.initials: M
authors.lastname: Zimmerman
authors.affiliation: Department of Mathematics and Computer Science, North Carolina Central University, Durham, NC, 27707, USA.
authors.forename: Gaolin Z
authors.initials: GZ
authors.lastname: Milledge
authors.affiliation: Department of Neurology, General Hospital of Ningxia Medical University, Ningxia Key Laboratory of Cerebrocranial Diseases, Incubation Base of National Key Laboratory, Yinchuan, 750004, China.|Department of Pharmaceutical Sciences, Biomanufacturing Research Institute Biotechnology Enterprise (BRITE), North Carolina Central University, Durham, NC, 27707, USA.
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Hou
authors.affiliation: Department of Neurology, General Hospital of Ningxia Medical University, Ningxia Key Laboratory of Cerebrocranial Diseases, Incubation Base of National Key Laboratory, Yinchuan, 750004, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Department of Neurology, General Hospital of Ningxia Medical University, Ningxia Key Laboratory of Cerebrocranial Diseases, Incubation Base of National Key Laboratory, Yinchuan, 750004, China. wangzhenhai1968@163.com.
authors.forename: Zhen-Hai
authors.initials: ZH
authors.lastname: Wang
authors.affiliation: Department of Pharmaceutical Sciences, Biomanufacturing Research Institute Biotechnology Enterprise (BRITE), North Carolina Central University, Durham, NC, 27707, USA. pli@nccu.edu.
authors.forename: P Andy
authors.initials: PA
authors.lastname: Li
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects* / Q000502:physiology; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D051379:Mice; D063154:Mitochondrial Dynamics / Q000187:drug effects* / Q000502:physiology; D012402:Rotenone / Q000633:toxicity*; D012995:Solubility; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D014867:Water / Q000378:metabolism
chemical_list: D012402:Rotenone; D014867:Water; D014451:Ubiquinone; C024989:coenzyme Q10
keywords: Fission; Fusion; Mitochondrial dynamics; Parkinson’s disease; Water-soluble Coenzyme Q10