title: Regenerative medicine for Parkinson's disease using differentiated nerve cells derived from human buccal fat pad stem cells.
abstract: The purpose of this study was to evaluate the utility of human adipose stem cells derived from the buccal fat pad (hBFP-ASCs) for nerve regeneration. Parkinson's disease (PD) is a neurodegenerative disorder characterized by progressive death of dopaminergic neurons. PD is a candidate disease for cell replacement therapy because it has no fundamental therapeutic methods. We examined the properties of neural-related cells induced from hBFP-ASCs as a cell source for PD treatment. hBFP-ASCs were cultured in neurogenic differentiation medium for about 2 weeks. After the morphology of hBFP-ASCs changed to neural-like cells, the medium was replaced with neural maintenance medium. Cells differentiated from hBFP-ASCs showed neuron-like structures and expressed neuron markers (β3-tubulin, neurofilament 200, and microtubule-associated protein 2), an astrocyte marker (glial fibrillary acidic protein), or dopaminergic neuron-related marker (tyrosine hydroxylase). Induced neural cells were transplanted into a 6-hydroxydopamine (6-OHDA)-lesioned rat hemi-parkinsonian model. At 4 weeks after transplantation, 6-OHDA-lesioned rats were subjected to apomorphine-induced rotation analysis. The transplanted cells survived in the brain of rats as dopaminergic neural cells. No tumor formation was found after cell transplantation. We demonstrated differentiation of hBFP-ASCs into neural cells, and that transplantation of these neural cells improved the symptoms of model rats. Our results suggest that neurons differentiated from hBFP-ASCs would be applicable to cell replacement therapy of PD.
authors.affiliation: Oral and Maxillofacial Surgery, Field of Oral and Maxillofacial Surgery and Systemic Medicine, Course of Clinical Science, Nippon Dental University Graduate School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan. tharuka@ngt.ndu.ac.jp.
authors.forename: Haruka
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: Department of NDU Life Sciences, Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, 102-8159, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ishikawa
authors.affiliation: Department of Oral and Maxillofacial Surgery, Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Tanaka
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001921:Brain / Q000166:cytology*; D002454:Cell Differentiation; D002470:Cell Survival; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D002478:Cells, Cultured; D002610:Cheek; D004195:Disease Models, Animal; D059290:Dopaminergic Neurons / Q000637:transplantation*; D006801:Humans; D009416:Nerve Regeneration / Q000502:physiology*; D010300:Parkinson Disease / Q000628:therapy*; D051381:Rats; D013234:Stem Cells / Q000166:cytology*
keywords: Buccal fat pad stem cells; Cell replacement therapy; Human adipose stem cells; Parkinson’s disease; Tissue stem cells