title: Sulfitobacter pontiacus subsp. fungiae subsp. nov., Isolated from Coral Fungia seychellensis from Andaman Sea, and Description of Sulfitobacter pontiacus subsp. pontiacus subsp. nov.
abstract: Two closely related aerobic, Gram reaction-negative rod-shaped bacteria (S7-75Tand S7-80) were isolated from mucus of coral Fungia seychellensis from Andaman Sea, India. Heterotrophic growth on marine agar was observed at 4-35 °C and pH 6.5-10.5; optimum growth occurred at 25-30 °C and pH 7-8. 16 S rRNA sequence analysis confirmed the strains belonged to the genus Sulfitobacter and the two isolates shared more than 99.28% pairwise sequence similarity. DNA-DNA similarity between two isolates S7-75Tand S7-80 was above 96%. Strain S7-75Tshowed maximum 16S rRNA similarity of 99.64% with Sulfitobacter pontiacus LMG 19752T. However, DNA-DNA relatedness between strain S7-75Tand S. pontiacus LMG 19752Tconfirmed the placement of strain S7-75Tas subspecies under the species S. pontiacus. Further, pulsed-field gel electrophoresis (PFGE), REP-PCR, ERIC-PCR fingerprint patterns and lipid profiles also differentiated strain S7-75Tfrom the reference strain of S. pontiacus LMG 19752T. The DNA G+C content was 59.8 mol%. Q10 was the major respiratory quinone. Based on polyphasic analysis, the isolate S7-75Trepresents a subspecies of S. pontiacus for which the name S. pontiacus subsp. fungiae subsp. nov. is proposed with S7-75T(=JCM 31094T= LMG 29158T) as type strain.
authors.affiliation: Institute of Life Sciences, Department of Biotechnology, Nalco Square, Bhubaneswar, 751023, India.
authors.forename: Sherin
authors.initials: S
authors.lastname: Zachariah
authors.affiliation: Institute of Life Sciences, Department of Biotechnology, Nalco Square, Bhubaneswar, 751023, India.
authors.forename: Prabla
authors.initials: P
authors.lastname: Kumari
authors.affiliation: Institute of Life Sciences, Department of Biotechnology, Nalco Square, Bhubaneswar, 751023, India. subratkdas@hotmail.com.
authors.forename: Subrata K
authors.initials: SK
authors.lastname: Das
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000382:microbiology*; D001482:Base Composition / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D006088:Gram-Negative Aerobic Bacteria / Q000145:classification / Q000235:genetics*; D041902:Rhodobacteraceae / Q000145:classification / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal