title: NMR metabolic fingerprints of murine melanocyte and melanoma cell lines: application to biomarker discovery.
abstract: Melanoma is the most aggressive type of skin cancer and efforts to improve the diagnosis of this neoplasia are largely based on the use of cell lines. Metabolomics is currently undergoing great advancements towards its use to screening for disease biomarkers. Although NMR metabolomics includes both 1D and 2D methodologies, there is a lack of data in the literature regarding heteronuclear 2D NMR assignments of the metabolome from eukaryotic cell lines. The present study applied NMR-based metabolomics strategies to characterize aqueous and lipid extracts from murine melanocytes and melanoma cell lines with distinct tumorigenic potential, successfully obtaining fingerprints of the metabolites from the extracts of the cell lines by means of 2D NMR HSQC correlation maps. Relative amounts of the identified metabolites were compared between the 4 cell lines. Multivariate analysis of1H NMR data was able not only to differentiate the melanocyte cell line from the tumorigenic ones but also distinguish among the 3 tumorigenic cell lines. We also investigated the effects of mitogenic agents, and found that they can markedly influence the metabolome of the melanocyte cell line, resembling the pattern of most proliferative cell lines.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Cx.P 19046, CEP 81531-990, Curitiba, PR, Brazil.
authors.forename: Arquimedes Paixão de
authors.initials: AP
authors.lastname: Santana-Filho
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Cx.P 19046, CEP 81531-990, Curitiba, PR, Brazil.
authors.forename: Thiago
authors.initials: T
authors.lastname: Jacomasso
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Cx.P 19046, CEP 81531-990, Curitiba, PR, Brazil.
authors.forename: Daniel Suss
authors.initials: DS
authors.lastname: Riter
authors.affiliation: Departamento de Química, Universidade Federal do Paraná, Cx.P. 19081, CEP 81531-990, Curitiba, PR, Brazil.
authors.forename: Andersson
authors.initials: A
authors.lastname: Barison
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Cx.P 19046, CEP 81531-990, Curitiba, PR, Brazil.
authors.forename: Marcello
authors.initials: M
authors.lastname: Iacomini
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Cx.P 19046, CEP 81531-990, Curitiba, PR, Brazil.
authors.forename: Sheila Maria Brochado
authors.initials: SM
authors.lastname: Winnischofer
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Cx.P 19046, CEP 81531-990, Curitiba, PR, Brazil.
authors.forename: Guilherme Lanzi
authors.initials: GL
authors.lastname: Sassaki
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D045744:Cell Line, Tumor; D005786:Gene Expression Regulation / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008544:Melanocytes / Q000187:drug effects / Q000378:metabolism*; D008546:Melanoma, Experimental / Q000235:genetics / Q000378:metabolism*; D055442:Metabolome; D055432:Metabolomics; D051379:Mice; D015999:Multivariate Analysis; D025341:Principal Component Analysis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D015415:Biomarkers; D013755:Tetradecanoylphorbol Acetate