title: Efficient siRNA-peptide conjugation for specific targeted delivery into tumor cells.
abstract: Despite the broad applicability of the Huisgen cycloaddition reaction, the click functionalization of RNAs with peptides still remains a challenge. Here we describe a straightforward method for the click functionalization of siRNAs with peptides of different sizes and complexities. Among them, a promising peptide carrier for the selective siRNA delivery into HER2+ breast cancer cell lines has been reported.
authors.affiliation: Department of Inorganic and Organic Chemistry, Section of Organic Chemistry, IBUB, University of Barcelona, Martí i Franquès 1-11, 08028 Barcelona, Spain.
authors.forename: Albert
authors.initials: A
authors.lastname: Gandioso
authors.affiliation: Department of Biology, University of Girona, Campus Montilivi, 17071 Girona, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Massaguer
authors.affiliation: Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Joint IRB-BSC Program in Computational Biology, Baldiri Reixac 10-12, 08028 Barcelona, Spain. montserrat.terrazas@irbbarcelona.org and The Join IRB-BSC Program in Computational Biology, Institute for Research in Biomedicine (IRB Barcelona), Spain.
authors.forename: Núria
authors.initials: N
authors.lastname: Villegas
authors.affiliation: Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Joint IRB-BSC Program in Computational Biology, Baldiri Reixac 10-12, 08028 Barcelona, Spain. montserrat.terrazas@irbbarcelona.org.
authors.forename: Cándida
authors.initials: C
authors.lastname: Salvans
authors.affiliation: Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Joint IRB-BSC Program in Computational Biology, Baldiri Reixac 10-12, 08028 Barcelona, Spain. montserrat.terrazas@irbbarcelona.org.
authors.forename: Dani
authors.initials: D
authors.lastname: Sánchez
authors.affiliation: Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Joint IRB-BSC Program in Computational Biology, Baldiri Reixac 10-12, 08028 Barcelona, Spain. montserrat.terrazas@irbbarcelona.org.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Brun-Heath
authors.affiliation: Department of Inorganic and Organic Chemistry, Section of Organic Chemistry, IBUB, University of Barcelona, Martí i Franquès 1-11, 08028 Barcelona, Spain.
authors.forename: Vicente
authors.initials: V
authors.lastname: Marchán
authors.affiliation: Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Joint IRB-BSC Program in Computational Biology, Baldiri Reixac 10-12, 08028 Barcelona, Spain. montserrat.terrazas@irbbarcelona.org and The Join IRB-BSC Program in Computational Biology, Institute for Research in Biomedicine (IRB Barcelona), Spain and Department of Biochemistry and Biomedicine, University of Barcelona, 08028 Barcelona, Spain.
authors.forename: Modesto
authors.initials: M
authors.lastname: Orozco
authors.affiliation: Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Joint IRB-BSC Program in Computational Biology, Baldiri Reixac 10-12, 08028 Barcelona, Spain. montserrat.terrazas@irbbarcelona.org.
authors.forename: Montserrat
authors.identifier: http://orcid.org/0000-0001-8614-5777
authors.initials: M
authors.lastname: Terrazas
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D057930:Click Chemistry; D004337:Drug Carriers / Q000737:chemistry / Q000493:pharmacokinetics; D005260:Female; D006801:Humans; D008968:Molecular Conformation; D010455:Peptides / Q000737:chemistry* / Q000493:pharmacokinetics*; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000493:pharmacokinetics*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics
chemical_list: D004337:Drug Carriers; D010455:Peptides; D034741:RNA, Small Interfering; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human