title: Cultivation of Podospora anserina on soybean hulls results in an efficient enzyme cocktail for plant biomass hydrolysis.
abstract: The coprophilic ascomycete fungus Podospora anserina was cultivated on three different plant biomasses, i.e. cotton seed hulls (CSH), soybean hulls (SBH) and acid-pretreated wheat straw (WS) for four days, and the potential of the produced enzyme mixtures was compared in the enzymatic saccharification of the corresponding lignocellulose feedstocks. The enzyme cocktail P. anserina produced after three days of growth on SBH showed superior capacity to release reducing sugars from all tested plant biomass feedstocks compared to the enzyme mixtures from CSH and WS cultures. Detailed proteomics analysis of the culture supernatants revealed that SBH contained the most diverse set of enzymes targeted on plant cell wall polymers and was particularly abundant in xylan, mannan and pectin acting enzymes. The importance of lytic polysaccharide monooxygenases (LPMOs) in plant biomass deconstruction was supported by identification of 20 out of 33 AA9 LPMOs in the SBH cultures. The results highlight the suitability of P. anserina as a source of plant cell wall degrading enzymes for biotechnological applications and the importance of selecting the most optimal substrate for the production of enzyme mixtures.
authors.affiliation: Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands; Department of Food and Environmental Sciences, Division of Microbiology and Biotechnology, P.O. Box 56, Viikinkaari 9, University of Helsinki, Helsinki, Finland, Finland.
authors.forename: Miia R
authors.initials: MR
authors.lastname: Mäkelä
authors.affiliation: Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands; Microbiology, Utrecht University, Padualaan 8, 3584 Ch Utrecht, The Netherlands.
authors.forename: Ourdia
authors.initials: O
authors.lastname: Bouzid
authors.affiliation: Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands; Brazilian Laboratory of Science and Technology of Bioethanol, Giuseppe Maximo Scolfaro 10.000, Campinas, Brazil.
authors.forename: Diogo
authors.initials: D
authors.lastname: Robl
authors.affiliation: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecules Research and Utrecht Institute for Pharmaceutical Sciences, Padualaan 8, 3584 CH Utrecht, The Netherlands; Netherlands Proteomics Center, Padualaan 8, 3584 CH Utrecht, The Netherlands.
authors.forename: Harm
authors.initials: H
authors.lastname: Post
authors.affiliation: Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.
authors.forename: Mao
authors.initials: M
authors.lastname: Peng
authors.affiliation: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecules Research and Utrecht Institute for Pharmaceutical Sciences, Padualaan 8, 3584 CH Utrecht, The Netherlands; Netherlands Proteomics Center, Padualaan 8, 3584 CH Utrecht, The Netherlands.
authors.forename: Albert
authors.initials: A
authors.lastname: Heck
authors.affiliation: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecules Research and Utrecht Institute for Pharmaceutical Sciences, Padualaan 8, 3584 CH Utrecht, The Netherlands; Netherlands Proteomics Center, Padualaan 8, 3584 CH Utrecht, The Netherlands.
authors.forename: Maarten
authors.initials: M
authors.lastname: Altelaar
authors.affiliation: Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands; Microbiology, Utrecht University, Padualaan 8, 3584 Ch Utrecht, The Netherlands. Electronic address: r.devries@cbs.knaw.nl.
authors.forename: Ronald P
authors.initials: RP
authors.lastname: de Vries
mesh_terms: D018533:Biomass; D001709:Biotechnology; D003368:Gossypium / Q000033:anatomy & histology / Q000378:metabolism; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism; D048468:Podospora / Q000201:enzymology* / Q000254:growth & development* / Q000378:metabolism; D013025:Glycine max / Q000033:anatomy & histology / Q000378:metabolism*; D014908:Triticum / Q000033:anatomy & histology / Q000378:metabolism
chemical_list: C036909:lignocellulose; D008031:Lignin
keywords: Auxiliary activities; Carbohydrate active enzymes; Plant biomass; Podospora anserina; Proteomics; Saccharification