title: Forkhead Box Protein J1 (FOXJ1) is Overexpressed in Colorectal Cancer and Promotes Nuclear Translocation of β-Catenin in SW620 Cells.
abstract: BACKGROUND FOXJ1, which is a forkhead transcription factor, has been previously studied mostly as a ciliary transcription factor. The role of FOXJ1 in cancer progression is still elusive and controversial. In the present study, the effect of FOXJ1 in progression of colorectal cancer (CRC) was investigated. MATERIAL AND METHODS The pattern of FOXJ1 expression was investigated using the method of immunohistochemistry (IHC) in a tissue microarray (TMA) incorporating 50 pairs of colon cancer specimens and adjacent normal tissue. In addition, the correlation of FOXJ1 expression with clinicopathological characteristics was evaluated in the other TMA containing 208 cases of colon cancer. Moreover, the influence of regulating FOXJ1 level on the proliferation, migration, and invasion ability of colorectal cancer (CRC) cells was evaluated. RESULTS Increased expression of FOXJ1was significantly associated with clinical stage (p<0.05), metastasis of lymph node (p<0.05), and invasion depth (p<0.001) in colon cancer, suggesting FOXJ1 is a tumor promoter in CRC. Consistently, FOXJ1 overexpression significantly enhanced the proliferation, migration, and invasion of CRC cells, while silencing of FOXJ1 induced the opposite effect. Furthermore, up-regulation of FOXJ1 in SW620 cells markedly inhibited the level of truncated APC and the phosphorylation of β-catenin, while the level of cyclinD1 was decreased. In addition, overexpression of FOXJ1 significantly promoted nuclear translocation of b-catenin in SW620 cells. CONCLUSIONS These findings demonstrate that increased FOXJ1 contributes to the progression of CRC, which might be associated with the promotion effect of b-catenin nuclear translocation. FOXJ1 may be a novel therapeutic target in CRC.
authors.affiliation: Department of Gastroenterology, Beijing Shijitan Hospital, Capital Medical University, Beijing, China (mainland).
authors.forename: Kuiliang
authors.initials: K
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology, Beijing Shijitan Hospital, Capital Medical University, Beijing, China (mainland).
authors.forename: Jianghao
authors.initials: J
authors.lastname: Fan
authors.affiliation: Department of Gastroenterology, Beijing Shijitan Hospital, Capital Medical University, Beijing, China (mainland).
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
mesh_terms: D021581:Active Transport, Cell Nucleus; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D018450:Disease Progression; D051858:Forkhead Transcription Factors / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D045325:HCT116 Cells; D006801:Humans; D007150:Immunohistochemistry; D046888:Tissue Array Analysis / Q000379:methods; D015854:Up-Regulation; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*
chemical_list: C495270:CTNNB1 protein, human; C106349:FOXJ1 protein, human; D051858:Forkhead Transcription Factors; D051176:beta Catenin