title: High Performance Liquid Chromatography-Diode Array Detector Method for the Simultaneous Determination of Five Compounds in the Pulp and Seed of Sea Buckthorn.
abstract: BACKGROUND
  Sea buckthorn (Hippophae rhamnoidesL.) as a traditional Chinese medicinal plant has various uses in Xinjiang.
  
  
  OBJECTIVE
  A reversed-phase rapid-resolution liquid-chromatography method with diode array detector was developed for simultaneous determination of protocatechuic acid, rutin, quercetin, kaempferol, and isorhamnetin in the pulp and seed of sea buckthorn, a widely used traditional Chinese medicine for promoting metabolism and treating scurvy and other diseases.
  
  
  METHODS
  Compounds were separated on an Agilent ZORBAX SB-C18 column (4.6 mm × 250 mm, 5 μm; USA) with gradient elution using methanol and 0.4% phosphoric acid (v/v) at 1.0 mL/min. Detection wavelength was set at 280 nm.
  
  
  METHODS
  The fruits of wild sea buckthorn were collected from Wushi County in Aksu, Xinjiang Province.
  The RSD of precision test of the five compounds were in the range of 0.60-2.22%, and the average recoveries ranged from 97.36% to 101.19%. Good linearity between specific chromatographic peak and component qualities were observed in the investigated ranges for all the analytes (R2> 0.9997).
  
  
  RESULTS
  The proposed method was successfully applied to determine the levels of five active components in sea buckthorn samples from Aksu in Xinjiang.
  
  
  CONCLUSIONS
  The proposed method is simple, fast, sensitive, accurate, and suitable for quantitative assessment of the pulp and seed of sea buckthorn.
  
  
  CONCLUSIONS
  Quantitative analysis method of protocatechuic acid, rutin, quercetin, kaempferol, and isorhamnetin in the extract of sea buckthorn pulp and seed is developed by high-performance liquid chromatography (HPLC) diode array detection.This method is simple and accurate; has strong specificity, good precision, and high recovery rate; and provides a reliable basis for further development of the substances in the pulp and seed of sea buckthorn.The method is widely used for content determination of active ingredients or physiologically active components in traditional Chinese medicine and its preparationAbbreviation used:PR: protocatechuic acid, RU: rutin, QU: quercetin, KA: kaempferol, IS: isorhamnetin, HPLC: high-performance liquid chromatography, HPLC-DAD: high performance liquid chromatographydiode array detector, LOD: linearity and limit of detection, LOQ: limit of quantitation, RSD: relative standard deviation.
authors.affiliation: College of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumqi, Xinjiang, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhao
authors.affiliation: College of TCM, Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: E
authors.initials: E
authors.lastname: Wen
authors.affiliation: Central Laboratory of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: Halmuart
authors.initials: H
authors.lastname: Upur
authors.affiliation: Central Laboratory of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: Shuge
authors.initials: S
authors.lastname: Tian
keywords: Content determination; HPLC-DAD; pulp; sea buckthorn; seed