title: Microbial Anti-Inflammatory Molecule (MAM) fromFaecalibacterium prausnitziiShows a Protective Effect on DNBS and DSS-Induced Colitis Model in Mice through Inhibition of NF-κB Pathway.
abstract: Faecalibacterium prausnitziiand its supernatant showed protective effects in different chemically-induced colitis models in mice. Recently, we described 7 peptides found in theF. prausnitziisupernatant, all belonging to a protein called Microbial Anti-inflammatory Molecule (MAM). These peptides were able to inhibit NF-κB pathwayin vitroand showed anti-inflammatory propertiesin vivoin a DiNitroBenzene Sulfate (DNBS)-induced colitis model. In this current proof we tested MAM effect on NF-κB pathwayin vivo, using a transgenic model of mice producing luciferase under the control of NF-κB promoter. Moreover, we tested this protein on Dextran Sodium Sulfate (DSS)-induced colitis in mice. To study the effect of MAM we orally administered to the mice aLactococcus lactisstrain carrying a plasmid containing the cDNA of MAM under the control of a eukaryotic promoter.L. lactisdelivered plasmids in epithelial cells of the intestinal membrane allowing thus the production of MAM directly by host. We showed that MAM administration inhibits NF-κB pathwayin vivo. We confirmed the anti-inflammatory properties of MAM in DNBS-induced colitis but also in DSS model. In DSS model MAM was able to inhibit Th1 and Th17 immune response while in DNBS model MAM reduced Th1, Th2, and Th17 immune response and increased TGFβ production.
authors.affiliation: Micalis Institute, Institut National de la Recherche Agronomique (INRA), AgroParisTech, Université Paris-Saclay Jouy-en-Josas, France.
authors.forename: Natalia M
authors.initials: NM
authors.lastname: Breyner
authors.affiliation: Micalis Institute, Institut National de la Recherche Agronomique (INRA), AgroParisTech, Université Paris-Saclay Jouy-en-Josas, France.
authors.forename: Cristophe
authors.initials: C
authors.lastname: Michon
authors.affiliation: Micalis Institute, Institut National de la Recherche Agronomique (INRA), AgroParisTech, Université Paris-SaclayJouy-en-Josas, France; Laboratorio de Genetica Celular e Molecular, Departamento de Microbiologia, Universidade Federal de Minas GeraisBelo Horizonte, Brazil.
authors.forename: Cassiana S
authors.initials: CS
authors.lastname: de Sousa
authors.affiliation: Micalis Institute, Institut National de la Recherche Agronomique (INRA), AgroParisTech, Université Paris-SaclayJouy-en-Josas, France; Laboratorio de Genetica Celular e Molecular, Departamento de Microbiologia, Universidade Federal de Minas GeraisBelo Horizonte, Brazil.
authors.forename: Priscilla B
authors.initials: PB
authors.lastname: Vilas Boas
authors.affiliation: Micalis Institute, Institut National de la Recherche Agronomique (INRA), AgroParisTech, Université Paris-Saclay Jouy-en-Josas, France.
authors.forename: Florian
authors.initials: F
authors.lastname: Chain
authors.affiliation: Laboratorio de Genetica Celular e Molecular, Departamento de Microbiologia, Universidade Federal de Minas Gerais Belo Horizonte, Brazil.
authors.forename: Vasco A
authors.initials: VA
authors.lastname: Azevedo
authors.affiliation: Micalis Institute, Institut National de la Recherche Agronomique (INRA), AgroParisTech, Université Paris-Saclay Jouy-en-Josas, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Langella
authors.affiliation: Micalis Institute, Institut National de la Recherche Agronomique (INRA), AgroParisTech, Université Paris-Saclay Jouy-en-Josas, France.
authors.forename: Jean M
authors.initials: JM
authors.lastname: Chatel
keywords: Faecalibacterium prausnitzii; Lactococcus lactis; MAM (Microbial Anti-inflammatory Molecule); NF-κB; colitis models; inflammation