title: Proper division plane orientation and mitotic progression together allow normal growth of maize.
abstract: How growth, microtubule dynamics, and cell-cycle progression are coordinated is one of the unsolved mysteries of cell biology. A maize mutant,tangled1, with known defects in growth and proper division plane orientation, and a recently characterized cell-cycle delay identified by time-lapse imaging, was used to clarify the relationship between growth, cell cycle, and proper division plane orientation. Thetangled1mutant was fully rescued by introduction of cortical division site localized TANGLED1-YFP. A CYCLIN1B destruction box was fused to TANGLED1-YFP to generate a line that mostly rescued the division plane defect but still showed cell-cycle delays when expressed in thetangled1mutant. Although an intermediate growth phenotype between wild-type and thetangled1mutant was expected, these partially rescued plants grew as well as wild-type siblings, indicating that mitotic progression delays alone do not alter overall growth. These data indicate that division plane orientation, together with proper cell-cycle progression, is critical for plant growth.
authors.affiliation: Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, CA 92521.|Biochemistry and Molecular Biology Graduate Program, University of California, Riverside, CA 92521.
authors.forename: Pablo
authors.identifier: 0000-0003-4846-4958
authors.initials: P
authors.lastname: Martinez
authors.affiliation: Department of Molecular Biology, University of Wyoming, Laramie, WY 82071.
authors.forename: Anding
authors.initials: A
authors.lastname: Luo
authors.affiliation: Department of Molecular Biology, University of Wyoming, Laramie, WY 82071.
authors.forename: Anne
authors.initials: A
authors.lastname: Sylvester
authors.affiliation: Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, CA 92521; carolyn.rasmussen@ucr.edu.
authors.forename: Carolyn G
authors.identifier: 0000-0002-4354-6295
authors.initials: CG
authors.lastname: Rasmussen
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics*; D002453:Cell Cycle / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics*; D002455:Cell Division / Q000235:genetics*; D056744:Cyclin B1 / Q000235:genetics*; D048749:Cytokinesis / Q000235:genetics; D008870:Microtubules / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D059008:Time-Lapse Imaging; D003313:Zea mays / Q000235:genetics / Q000254:growth & development*
chemical_list: D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; D056744:Cyclin B1; C525946:TANGLED protein, Arabidopsis
keywords: TANGLED; cell cycle; division; maize; phragmoplast