title: Transcriptional and Biochemical Alterations in Zebrafish Eleuthero-Embryos (Danio rerio) After Exposure to Synthetic Progestogen Dydrogesterone.
abstract: Little information has so far been known on the effects of synthetic progestogen dydrogesterone (DDG) in organisms like fish. This study aimed to investigate the effects of DDG on the transcriptional and biochemical alterations in zebrafish eleuthero-embryos. Zebrafish eleuthero-embryos were analyzed for the transcriptional alterations by real-time quantitative PCR (RT-qPCR) and biochemical changes by attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FITR) after 144 h exposure to DDG. The results of qPCR analysis showed that DDG exposure significantly suppressed the transcriptions of target genes involved in hypothalamic-pituitary-thyroid (HPT) axis, while it induced the expression of target genes mRNA belonging to hypothalamic-pituitary-gonad (HPG) axis. In addition, ATR-FTIR spectroscopy analysis showed that the biochemical alterations of protein, nucleic acid and lipid were observed following DDG treatment. The finding from this study suggests that DDG exposure could have potential multiple effects in fish.
authors.affiliation: State Key Laboratory of Organic Geochemistry, CAS Research Centre of PRD Environmental Pollution and Control, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, 510640, Guangzhou, China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Organic Geochemistry, CAS Research Centre of PRD Environmental Pollution and Control, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, 510640, Guangzhou, China. guangguo.ying@gmail.com.
authors.forename: Guang-Guo
authors.identifier: http://orcid.org/0000-0002-3387-1078
authors.initials: GG
authors.lastname: Ying
authors.affiliation: State Key Laboratory of Organic Geochemistry, CAS Research Centre of PRD Environmental Pollution and Control, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, 510640, Guangzhou, China.
authors.forename: Guo-Yong
authors.initials: GY
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Organic Geochemistry, CAS Research Centre of PRD Environmental Pollution and Control, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, 510640, Guangzhou, China.|School of Chemistry and Environment, Guangdong Ocean University, 524088, Zhanjiang, China.
authors.forename: Yan-Qiu
authors.initials: YQ
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Organic Geochemistry, CAS Research Centre of PRD Environmental Pollution and Control, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, 510640, Guangzhou, China.
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Organic Geochemistry, CAS Research Centre of PRD Environmental Pollution and Control, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, 510640, Guangzhou, China.
authors.forename: Jian-Liang
authors.initials: JL
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Organic Geochemistry, CAS Research Centre of PRD Environmental Pollution and Control, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, 510640, Guangzhou, China.
authors.forename: Jin-Na
authors.initials: JN
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004394:Dydrogesterone / Q000633:toxicity*; D028001:Eleutherococcus; D004625:Embryo, Nonmammalian / Q000187:drug effects*; D052244:Endocrine Disruptors / Q000633:toxicity*; D004703:Endocrine System; D045167:Progesterone Congeners; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*; D015027:Zebrafish / Q000196:embryology* / Q000378:metabolism
chemical_list: D052244:Endocrine Disruptors; D045167:Progesterone Congeners; D014874:Water Pollutants, Chemical; D004394:Dydrogesterone
keywords: Biochemical alteration; Dydrogesterone; Transcription; Zebrafish