title: Salivary oxidative status in patients with oral lichen planus.
abstract: Reactive oxygen species (ROS) are involved in the pathogenesis of many inflammatory diseases, including oral lichen planus. Therefore, determining the salivary markers of oxidative stress is an excellent alternative approach to diagnosing oral cavity diseases. The objective of our study was to provide preliminary validation and determination of the salivary markers of oxidative stress in both patients with reticular and erosive forms of oral lichen planus as well as in healthy individuals without any oral lesions. In total, 62 patients with oral lichen planus (OLP) were enrolled in the study, including 31 with the reticular form of lichen planus (44.63 ± 11.05 years) and 31 with erosive forms (40.43 ± 10.05 years), who had never been treated for their disease. The control group comprised 30 individuals without any oral lesions (42.12 ± 12.22 years). We determined the saliva levels in glutathione (GSH), total antioxidant capacity (TAC), and thiobarbituric acid reactive substances (TBARS). The mean saliva levels of GSH and TAC were significantly lower (P < 0.01) in OLP patients compared to the control group. The mean levels of salivary TBARS were higher in both OLP groups (reticular and erosive) compared to the control group (P = 0.01). The lower saliva levels of GSH and TAC in patients with OLP indicate that free radicals and the resulting oxidative damage may play an important role in the pathogenesis of OLP lesions. In conclusion, monitoring the oxidant-antioxidant status of saliva may serve as an efficient and less intrusive marker for determining stages of disease development in patients with OLP.
authors.affiliation: Department of Periodontology and Oral Medicine, Jagiellonian University Medical College, Cracow, Poland.
authors.forename: D
authors.initials: D
authors.lastname: Darczuk
authors.affiliation: Department of Medical Diagnostics, Faculty of Pharmacy, Jagiellonian University Medical College, Cracow, Poland.
authors.forename: W
authors.initials: W
authors.lastname: Krzysciak
authors.affiliation: Student Scientific Association at the Department of Medical Diagnostics, Jagiellonian University Medical College, Poland.
authors.forename: P
authors.initials: P
authors.lastname: Vyhouskaya
authors.affiliation: Department of Periodontology and Oral Medicine, Jagiellonian University Medical College, Cracow, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Kesek
authors.affiliation: Department of Periodontology and Oral Medicine, Jagiellonian University Medical College, Cracow, Poland.
authors.forename: D
authors.initials: D
authors.lastname: Galecka-Wanatowicz
authors.affiliation: Department of Periodontology and Oral Medicine, Jagiellonian University Medical College, Cracow, Poland.
authors.forename: W
authors.initials: W
authors.lastname: Lipska
authors.affiliation: Department of Oral Surgery, Jagiellonian University Medical College, Cracow, Poland.
authors.forename: T
authors.initials: T
authors.lastname: Kaczmarzyk
authors.affiliation: Department of Pharmacobiology, Faculty of Pharmacy, Jagiellonian University Medical College, Cracow, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Gluch-Lutwin
authors.affiliation: Department of Pharmacobiology, Faculty of Pharmacy, Jagiellonian University Medical College, Cracow, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Mordyl
authors.affiliation: Department of Periodontology and Oral Medicine, Jagiellonian University Medical College, Cracow, Poland. mdgajews@cyf-kr.edu.pl.
authors.forename: M
authors.initials: M
authors.lastname: Chomyszyn-Gajewska
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D017676:Lichen Planus, Oral / Q000378:metabolism* / Q000503:physiopathology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D012463:Saliva / Q000378:metabolism* / Q000502:physiology*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D017392:Thiobarbituric Acid Reactive Substances; D008315:Malondialdehyde; D005978:Glutathione