title: 13C labeling analysis of sugars by high resolution-mass spectrometry for metabolic flux analysis.
abstract: Metabolic flux analysis is particularly complex in plant cells because of highly compartmented metabolism. Analysis of free sugars is interesting because it provides data to define fluxes around hexose, pentose, and triose phosphate pools in different compartment. In this work, we present a method to analyze the isotopomer distribution of free sugars labeled with carbon 13 using a liquid chromatography-high resolution mass spectrometry, without derivatized procedure, adapted for Metabolic flux analysis. Our results showed a good sensitivity, reproducibility and better accuracy to determine isotopic enrichments of free sugars compared to our previous methods [5, 6].
authors.affiliation: Sorbonne Universités, Génie Enzymatique et Cellulaire, FRE CNRS 3580, Université de Technologie de Compiègne, 60203 Compiègne Cedex, France. Electronic address: sebastien.acket@utc.fr.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Acket
authors.affiliation: Sorbonne Universités, Génie Enzymatique et Cellulaire, FRE CNRS 3580, Université de Technologie de Compiègne, 60203 Compiègne Cedex, France.
authors.forename: Anthony
authors.initials: A
authors.lastname: Degournay
authors.affiliation: Sorbonne Universités, Génie Enzymatique et Cellulaire, FRE CNRS 3580, Université de Technologie de Compiègne, 60203 Compiègne Cedex, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Merlier
authors.affiliation: Sorbonne Universités, Génie Enzymatique et Cellulaire, FRE CNRS 3580, Université de Technologie de Compiègne, 60203 Compiègne Cedex, France.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Thomasset
mesh_terms: D002247:Carbon Isotopes; D002853:Chromatography, Liquid; D019597:Flax / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D005632:Fructose / Q000096:biosynthesis / Q000302:isolation & purification; D005947:Glucose / Q000096:biosynthesis / Q000302:isolation & purification; D007553:Isotope Labeling / Q000379:methods*; D008320:Maltose / Q000096:biosynthesis / Q000302:isolation & purification; D013058:Mass Spectrometry; D064688:Metabolic Flux Analysis / Q000379:methods*; D011887:Raffinose / Q000096:biosynthesis / Q000302:isolation & purification; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D012680:Sensitivity and Specificity; D013395:Sucrose / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D002247:Carbon Isotopes; D005632:Fructose; D013395:Sucrose; D008320:Maltose; D005947:Glucose; D011887:Raffinose
keywords: 13C-labeling; Isotopomers; LC-HRMS; Mass spectrometry; Metabolic flux analysis; Sugars