title: An overview of the infection of CMV, HSV 1/2 and EBV in Mexican patients with glioblastoma multiforme.
abstract: Several risk factors are involved in glioblastoma, including cytomegalovirus (CMV). This research was carried out to determine the rate of CMV infection, as well as HSV 1/2 and EBV in brain tissue, in patients with glioblastomamultiforme (GBM). The tissues were tested using immunohistochemistry, PCR, in situ hybridization and real-time PCR. At least, one HHV was detected in 21/29 (72%) patients as follows: single infections with HSV-1/2 in 4/21 (19%), EBV in 6/21 (28.6%) and CMV in 1/21 (4.8%). Mixed viral infection, HSV-1/2 and EBV were detected in 4/21 patients (19%), CMV and EBV in 5/21 (23.8%), and HSV-1/2, EBV, and CMV in 1/21. The CMV viral load ranged from 3×102to 4.33×105genome/100ng of tissue. Genotype based on CMV gB was 3/7 where 2/3 was gB1 and 1/3 gB4. HSV, EBV and CMV were frequently found in brain tissues, more in mix in a population reported as highly seropositive.
authors.affiliation: Área de Virología, Laboratorio de Infectología, Hospital Infantil de México Federico Gómez, Mexico; Laboratorio de Bacteriología Médica, Departamento de Microbiología y Programa de Doctorado en Ciencias en Biomedicina y Biotecnología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico; Departamento de Neuropatología, Instituto Nacional de Neurología Manuel Velazco Suárez, Mexico.
authors.forename: Sergio
authors.initials: S
authors.lastname: Zavala-Vega
authors.affiliation: Laboratorio de Bacteriología Médica, Departamento de Microbiología y Programa de Doctorado en Ciencias en Biomedicina y Biotecnología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico.
authors.forename: Graciela
authors.initials: G
authors.lastname: Castro-Escarpulli
authors.affiliation: Área de Virología, Laboratorio de Infectología, Hospital Infantil de México Federico Gómez, Mexico.
authors.forename: Hector
authors.initials: H
authors.lastname: Hernández-Santos
authors.affiliation: Departamento de Neuropatología, Instituto Nacional de Neurología Manuel Velazco Suárez, Mexico.
authors.forename: Citlatepetl
authors.initials: C
authors.lastname: Salinas-Lara
authors.affiliation: Laboratorio de Morfología Molecular, Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico.
authors.forename: Icela
authors.initials: I
authors.lastname: Palma
authors.affiliation: Departamento de Epidemiología Clínica, Hospital de Pediatría Centro Médico Nacional Siglo XXI Instituto Mexicano del Seguro Social, Mexico.
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Mejía-Aranguré
authors.affiliation: Departamento de Neuropatología, Instituto Nacional de Neurología Manuel Velazco Suárez, Mexico.
authors.forename: Noemí
authors.initials: N
authors.lastname: Gelista-Herrera
authors.affiliation: Departamento de Neuropatología, Instituto Nacional de Neurología Manuel Velazco Suárez, Mexico.
authors.forename: Daniel
authors.initials: D
authors.lastname: Rembao-Bojorquez
authors.affiliation: Laboratorio de Investigación en Bacteriología Intestinal, Hospital Infantil de México Federico Gómez, Mexico.
authors.forename: Sara A
authors.initials: SA
authors.lastname: Ochoa
authors.affiliation: Laboratorio de Investigación en Bacteriología Intestinal, Hospital Infantil de México Federico Gómez, Mexico.
authors.forename: Ariadnna
authors.initials: A
authors.lastname: Cruz-Córdova
authors.affiliation: Laboratorio de Investigación en Bacteriología Intestinal, Hospital Infantil de México Federico Gómez, Mexico.
authors.forename: Juan
authors.initials: J
authors.lastname: Xicohtencatl-Cortes
authors.affiliation: Área de Virología, Laboratorio de Infectología, Hospital Infantil de México Federico Gómez, Mexico.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Uribe-Gutiérrez
authors.affiliation: Área de Virología, Laboratorio de Infectología, Hospital Infantil de México Federico Gómez, Mexico. Electronic address: jose.arellano@salud.gob.mx.
authors.forename: José
authors.initials: J
authors.lastname: Arellano-Galindo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001921:Brain / Q000821:virology; D001932:Brain Neoplasms / Q000821:virology*; D003430:Cross-Sectional Studies; D003587:Cytomegalovirus / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000150:complications*; D020031:Epstein-Barr Virus Infections / Q000150:complications*; D005260:Female; D005909:Glioblastoma / Q000821:virology*; D006561:Herpes Simplex / Q000150:complications*; D018259:Herpesvirus 1, Human / Q000302:isolation & purification; D004854:Herpesvirus 4, Human / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008800:Mexico; D008875:Middle Aged; D012189:Retrospective Studies; D012307:Risk Factors; D019562:Viral Load; D055815:Young Adult
keywords: Cytomegalovirus; Epstein–Barr; Glioblastomamultiforme; HHVs; Herpes; Viral infection