title: Comparative RNA-Sequence Transcriptome Analysis of Phenolic Acid Metabolism inSalvia miltiorrhiza, a Traditional Chinese Medicine Model Plant.
abstract: Salvia miltiorrhizaBunge is an important traditional Chinese medicine (TCM). In this study, twoS. miltiorrhizagenotypes (BH18 and ZH23) with different phenolic acid concentrations were used for de novo RNA sequencing (RNA-seq). A total of 170,787 transcripts and 56,216 unigenes were obtained. There were 670 differentially expressed genes (DEGs) identified between BH18 and ZH23, 250 of which were upregulated in ZH23, with genes involved in the phenylpropanoid biosynthesis pathway being the most upregulated genes. Nine genes involved in the lignin biosynthesis pathway were upregulated in BH18 and thus result in higher lignin content in BH18. However, expression profiles of most genes involved in the core common upstream phenylpropanoid biosynthesis pathway were higher in ZH23 than that in BH18. These results indicated that genes involved in the core common upstream phenylpropanoid biosynthesis pathway might play an important role in downstream secondary metabolism and demonstrated that lignin biosynthesis was a putative partially competing pathway with phenolic acid biosynthesis. The results of this study expanded our understanding of the regulation of phenolic acid biosynthesis inS. miltiorrhiza.
authors.affiliation: Agronomy College, Shandong Agricultural University, Tai'an, Shandong 271028, China; State Key Laboratory of Crop Biology, Agronomy College, Shandong Agricultural University, Tai'an, Shandong 271018, China.
authors.forename: Zhenqiao
authors.initials: Z
authors.lastname: Song
authors.affiliation: Agronomy College, Shandong Agricultural University, Tai'an, Shandong 271028, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Guo
authors.affiliation: Agronomy College, Shandong Agricultural University, Tai'an, Shandong 271028, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Crop Biology, Agronomy College, Shandong Agricultural University, Tai'an, Shandong 271018, China.
authors.forename: Caicai
authors.initials: C
authors.lastname: Lin
authors.affiliation: Agronomy College, Shandong Agricultural University, Tai'an, Shandong 271028, China; State Key Laboratory of Crop Biology, Agronomy College, Shandong Agricultural University, Tai'an, Shandong 271018, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Wang
authors.affiliation: Agronomy College, Shandong Agricultural University, Tai'an, Shandong 271028, China; State Key Laboratory of Crop Biology, Agronomy College, Shandong Agricultural University, Tai'an, Shandong 271018, China.
authors.forename: Xingfeng
authors.identifier: 0000-0003-1943-7328
authors.initials: X
authors.lastname: Li