title: Antimicrobial and antioxidant activities of Flammulina velutipes polysaccharides and polysaccharide-iron(III) complex [corrected].
abstract: FVP is polysacchrides obtained from Flammulina velutipes. A polysacchride named FVP2 was isolated from FVP by DEAE cellulose-52 chromatography and Sephadex G-100 size-exclusion chromatography. FVP-Fe and FVP2-Fe were synthesized by neutralization of FeCl3carbohydrate solution. The antibacterial and antifungal activities of FVP, FVP2, FVP-Fe, FVP2-Fe were investigated and their antioxidant effects on hydroxyl, 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide anion, 2,2'-azobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals, reducing power, inhibition of malondialdehyde (MDA) were assessed in vitro. The results suggested that FVP-Fe and FVP2-Fe significantly suppressed the growth of bacteria Staphylococcus aureus, Escherichia coli, and Bacillus subtilis, and have relatively strong antioxidant activity to scavenge superoxide anion radical. In addition, FVP exhibited strong antioxidant activity to eliminate hydroxyl, DPPH, ABTS radicals, had high reducing power and inhibited the MDA production of health mice liver homogenate induced by auto-oxidation and Fe2+-H2O2system.
authors.affiliation: Department of Food Quality and Safety, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 210009, China.
authors.forename: Ya-Ru
authors.initials: YR
authors.lastname: Dong
authors.affiliation: Department of Food Quality and Safety, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 210009, China.
authors.forename: Shu-Jie
authors.initials: SJ
authors.lastname: Cheng
authors.affiliation: Department of Food Quality and Safety, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 210009, China.
authors.forename: Guo-Hong
authors.initials: GH
authors.lastname: Qi
authors.affiliation: Department of Food Quality and Safety, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 210009, China.
authors.forename: Zhi-Ping
authors.initials: ZP
authors.lastname: Yang
authors.affiliation: Department of Food Quality and Safety, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 210009, China.
authors.forename: Shi-Yu
authors.initials: SY
authors.lastname: Yin
authors.affiliation: Department of Food Quality and Safety, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 210009, China. Electronic address: cpucgt@cpu.edu.cn.
authors.forename: Gui-Tang
authors.initials: GT
authors.lastname: Chen
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects*; D005290:Ferric Compounds / Q000494:pharmacology*; D055393:Flammulina / Q000737:chemistry*; D008099:Liver / Q000187:drug effects; D051379:Mice; D011134:Polysaccharides / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D005290:Ferric Compounds; D011134:Polysaccharides
keywords: 2,2′-Azobis(3-ethylbenzothiazoline-6-sulfonic acid) (PubChem CID: 73857778; Antimicrobial activity; Antioxidant activity; DPPH, Free radical (PubChem CID: 2735032); Flammulina velutipes polysaccharides; Hydroxyl (PubChem CID: 961); Iron chloride (FeCl(3)) (PubChem CID: 24380); Malondialdehyde (PubChem CID: 10964); Polysacchride-iron (III) complex; Superoxide anion (PubChem CID: 5359597)