title: LC-MS/MS measurement of ampicillin residue in chicken tissues at 2 days after in-feed administration.
abstract: We assessed ampicillin (ABPC) concentrations of liver, kidney and skin at a 2-day withdrawal period in ten male and ten female White Leghorn chickens fed the diet containing ABPC (ABPC medicated feed 40 mg/kg body weight/day) for a week. The ABPC residues were measured with liquid chromatography-tandem mass spectrometry and the mean recoveries and quantitation limits ranged from 93.0% to 102.7% and from 0.1 to 1.4 ng/g, respectively. The residual ABPC concentrations were ≤7.82 ng/g for the skin and ≤0.64 ng/g for the kidney, suggesting below the Japanese provisional maximum residue limits. These results revealed that the analytical method is developed for residue ABPC and that the withdrawal period is appropriate.
authors.affiliation: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1-15-1, Tokura, Kokubunji, Tokyo 185-8511, Japan.
authors.forename: Kouko
authors.initials: K
authors.lastname: Hamamoto
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Mizuno
mesh_terms: D000667:Ampicillin / Q000008:administration & dosage / Q000493:pharmacokinetics*; D000821:Animal Feed; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D002645:Chickens; D002853:Chromatography, Liquid / Q000662:veterinary*; D019587:Dietary Supplements; D004350:Drug Residues / Q000493:pharmacokinetics*; D005260:Female; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D012867:Skin / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000662:veterinary*
chemical_list: D000900:Anti-Bacterial Agents; D000667:Ampicillin