title: A universal aptameric biosensor: Multiplexed detection of small analytes via aggregated perylene-based broad-spectrum quencher.
abstract: A universal aptameric system based on the taking advantage of double-stranded DNA/perylene diimide (dsDNA/PDI) as the signal probe was developed for multiplexed detection of small molecules. Aptamers are single-stranded DNA or RNA oligonucleotides which are selected in vitro by a process known as systematic evolution of ligands by exponential enrichment. In this work, we synthesized a new kind of PDI and reported this aggregated PDI could quench the double-stranded DNA (dsDNA)-labeled fluorophores with a high quenching efficiency. The quenching efficiencies on the fluorescence of FAM, TAMRA and Cy5 could reach to 98.3%±0.9%, 97.2%±0.6% and 98.1%±1.1%, respectively. This broad-spectrum quencher was then adopted to construct a multicolor biosensor via a label-free approach. A structure-switching-triggered enzymatic recycling amplification was employed for signal amplification. High quenching efficiency combined with autocatalytic target recycling amplification afforded the biosensor with high sensitivity towards small analytes. For other targets, changing the corresponding aptamer can achieve the goal. The quencher did not interfere with the catalytic activity of nuclease. The biosensor could be manipulated with similar sensitivity no matter in pre-addition or post-addition manner. Moreover, simultaneous and multiplexed analysis of several small molecules in homogeneous solution was achieved, demonstrating its potential application in the rapid screening of multiple biotargets.
authors.affiliation: College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650092, Yunnan, PR China. Electronic address: hudierong_168@163.com.
authors.forename: Rong
authors.initials: R
authors.lastname: Hu
authors.affiliation: College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650092, Yunnan, PR China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650092, Yunnan, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Molecular Engineering for Theronastics, Hunan University, Changsha 410082, China.
authors.forename: Dan-Qing
authors.initials: DQ
authors.lastname: Lu
authors.affiliation: College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650092, Yunnan, PR China.
authors.forename: Yun-Hui
authors.initials: YH
authors.lastname: Yang
authors.affiliation: College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650092, Yunnan, PR China.
authors.forename: Quan-Qing
authors.initials: QQ
authors.lastname: Xu
authors.affiliation: College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650092, Yunnan, PR China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Ruan
authors.affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Molecular Engineering for Theronastics, Hunan University, Changsha 410082, China.
authors.forename: Liu-Ting
authors.initials: LT
authors.lastname: Mo
authors.affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Molecular Engineering for Theronastics, Hunan University, Changsha 410082, China.
authors.forename: Xiao-Bing
authors.initials: XB
authors.lastname: Zhang
mesh_terms: D000241:Adenosine / Q000032:analysis* / Q000097:blood; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D002232:Carbocyanines / Q000737:chemistry; D003042:Cocaine / Q000032:analysis* / Q000097:blood; D018765:Dopamine Uptake Inhibitors / Q000032:analysis* / Q000097:blood; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D007094:Imides / Q000737:chemistry*; D057230:Limit of Detection; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D052157:Aptamers, Nucleotide; D002232:Carbocyanines; D018765:Dopamine Uptake Inhibitors; D005456:Fluorescent Dyes; D007094:Imides; C085321:cyanine dye 5; C521332:perylenediimide; D010569:Perylene; D003042:Cocaine; D000241:Adenosine
keywords: Aptamers; Broad-spectrum quencher; Multiplexed detection; Perylene diimide; Small molecules