title: The performance efficiency of bioaugmentation to prevent anaerobic digestion failure from ammonia and propionate inhibition.
abstract: This study aims to investigate the effect of bioaugmentation with enriched methanogenic propionate degrading microbial consortia on propionate fermentation under ammonia stress from total ammonia nitrogen concentration (TAN) of 3.0gNL-1. Results demonstrated that bioaugmentation could prevent unstable digestion against further deterioration. After 45days of 1dosage (0.3g dry cell weight L-1d-1, DCW L-1d-1) of bioaugmentation, the average volumetric methane production (VMP), methane recovery rate and propionic acid (HPr) degradation rate was enhanced by 70mLL-1d-1, 21% and 51%, respectively. In contrast, the non-bioaugmentation reactor almost failed. Routine addition of a double dosage (0.6g DCW L-1d-1) of bioaugmentation culture was able to effectively recover the failing digester. The results of FISH suggested that the populations of Methanosaetaceae increased significantly, which could be a main contributor for the positive effect on methane production.
authors.affiliation: College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China; Laboratory of Biomass Bio-chemical Conversion, GuangZhou Institute of Energy Conversion, Chinese Academy of Sciences, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Faculty of Engineering and the Environment, University of Southampton, Southampton SO17 1BJ, UK.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Laboratory of Biomass Bio-chemical Conversion, GuangZhou Institute of Energy Conversion, Chinese Academy of Sciences, PR China.
authors.forename: Yongming
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Key Laboratory of Clean Utilization Technology for Renewable Energy in Ministry of Agriculture, College of Engineering, China Agricultural University, Beijing 100083, PR China.
authors.forename: Shubiao
authors.initials: S
authors.lastname: Wu
authors.affiliation: Laboratory of Biomass Bio-chemical Conversion, GuangZhou Institute of Energy Conversion, Chinese Academy of Sciences, PR China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Kong
authors.affiliation: Laboratory of Biomass Bio-chemical Conversion, GuangZhou Institute of Energy Conversion, Chinese Academy of Sciences, PR China.
authors.forename: Zhenhong
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Clean Utilization Technology for Renewable Energy in Ministry of Agriculture, College of Engineering, China Agricultural University, Beijing 100083, PR China. Electronic address: rjdong@cau.edu.cn.
authors.forename: Renjie
authors.initials: R
authors.lastname: Dong
mesh_terms: D000641:Ammonia / Q000494:pharmacology*; D000693:Anaerobiosis / Q000187:drug effects; D001419:Bacteria / Q000187:drug effects / Q000235:genetics; D001673:Biodegradation, Environmental / Q000187:drug effects; D019149:Bioreactors / Q000382:microbiology; D005285:Fermentation / Q000187:drug effects; D017404:In Situ Hybridization, Fluorescence; D008697:Methane / Q000096:biosynthesis; D059013:Microbial Consortia / Q000187:drug effects; D011422:Propionates / Q000494:pharmacology*; D012037:Refuse Disposal / Q000379:methods*
chemical_list: D011422:Propionates; D000641:Ammonia; D008697:Methane
keywords: Ammonia inhibition; Bioaugmentation; Microbial community; Propionate degradation; Propionate-oxidizing bacteria