title: Sulforaphane Prevents Angiotensin II-Induced Testicular Cell Death via Activation of NRF2.
abstract: Although angiotensin II (Ang II) was reported to facilitate sperm motility and intratesticular sperm transport, recent findings shed light on the efficacy of Ang II in stimulating inflammatory events in testicular peritubular cells, effect of which may play a role in male infertility. It is still unknown whether Ang II can induce testicular apoptotic cell death, which may be a more direct action of Ang II in male infertility. Therefore, the present study aims to determine whether Ang II can induce testicular apoptotic cell death and whether this action can be prevented by sulforaphane (SFN) via activating nuclear factor (erythroid-derived 2)-like 2 (NRF2), the governor of antioxidant-redox signalling. Eight-week-old male C57BL/6J wild type (WT) andNrf2gene knockout mice were treated with Ang II, in the presence or absence of SFN. In WT mice, SFN activated testicular NRF2 expression and function, along with a marked attenuation in Ang II-induced testicular oxidative stress, inflammation, endoplasmic reticulum stress, and apoptotic cell death. Deletion of theNrf2gene led to a complete abolishment of these efficacies of SFN. The present study indicated that Ang II may result in testicular apoptotic cell death, which can be prevented by SFN via the activation of NRF2.
authors.affiliation: Department of Urology, China-Japan Union Hospital of Jilin University, 126 Xiantai St, Changchun, Jilin 130033, China; Pediatric Research Institute, Department of Pediatrics, Wendy L. Novak Diabetes Care Center, University of Louisville, 570 S Preston St, Louisville, KY 40202, USA.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Nephrology, The Second Hospital of Jilin University, 218 Ziqiang St, Changchun, Jilin 130041, China.
authors.forename: Hao
authors.identifier: 0000-0001-5738-4015
authors.initials: H
authors.lastname: Wu
authors.affiliation: Pediatric Research Institute, Department of Pediatrics, Wendy L. Novak Diabetes Care Center, University of Louisville, 570 S Preston St, Louisville, KY 40202, USA; The Key Laboratory of Pathobiology, Ministry of Education, The Norman Bethune Medical College, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xin
authors.affiliation: Pediatric Research Institute, Department of Pediatrics, Wendy L. Novak Diabetes Care Center, University of Louisville, 570 S Preston St, Louisville, KY 40202, USA; Cardiovascular Center, The First Hospital of Jilin University, 71 Xinmin St, Changchun, Jilin 130021, China.
authors.forename: Yang
authors.identifier: 0000-0002-9302-9464
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Pediatric Research Institute, Department of Pediatrics, Wendy L. Novak Diabetes Care Center, University of Louisville, 570 S Preston St, Louisville, KY 40202, USA; Department of Nephrology, The First Hospital of Jilin University, 71 Xinmin St, Changchun, Jilin 130021, China.
authors.forename: Lili
authors.identifier: 0000-0002-0674-1155
authors.initials: L
authors.lastname: Kong
authors.affiliation: Pediatric Research Institute, Department of Pediatrics, Wendy L. Novak Diabetes Care Center, University of Louisville, 570 S Preston St, Louisville, KY 40202, USA; Chinese-American Research Institute for Diabetic Complications, School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Department of Nephrology, China-Japan Union Hospital of Jilin University, Changchun, China, 126 Xiantai St, Changchun, Jilin 130033, China.
authors.forename: Feng
authors.identifier: 0000-0003-4383-0939
authors.initials: F
authors.lastname: Liu
authors.affiliation: Pediatric Research Institute, Department of Pediatrics, Wendy L. Novak Diabetes Care Center, University of Louisville, 570 S Preston St, Louisville, KY 40202, USA; Chinese-American Research Institute for Diabetic Complications, School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors.forename: Lu
authors.identifier: 0000-0003-3048-1135
authors.initials: L
authors.lastname: Cai
mesh_terms: D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D015153:Blotting, Western; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D007248:Infertility, Male; D017879:Isothiocyanates / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D051267:NF-E2-Related Factor 2 / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013454:Sulfoxides; D013737:Testis / Q000187:drug effects*
chemical_list: D016588:Anticarcinogenic Agents; D017879:Isothiocyanates; D051267:NF-E2-Related Factor 2; C495636:Nfe2l2 protein, mouse; D013454:Sulfoxides; D000804:Angiotensin II; C016766:sulforaphane