title: Stepwise morphological changes and cytoskeletal reorganization of human mesenchymal stem cells treated by short-time cyclic uniaxial stretch.
abstract: This study aimed to investigate stepwise remodeling of human mesenchymal stem cells (hMSCs) in response to cyclic stretch through rearrangement and alignment of cells and cytoskeleton regulation toward smooth muscle cell (SMC) fate in different time spans. Image analysis techniques were utilized to calculate morphological parameters. Cytoskeletal reorganization was observed by investigating F-actin filaments using immunofluorescence staining, and expression level of contractile SMC markers was followed by a quantitative polymerase chain reaction method. Applying cyclic uniaxial stretch on cultured hMSCs, utilizing a costume-made device, led to alteration in fractal dimension (FD) and cytoskeleton structure toward continuous alignment and elongation of cells by elevation of strain duration. Actin filaments became more aligned perpendicular to the axis of mechanical stretch by increasing uniaxial loading duration. At first, FD met a significant decrease in 4 h loading duration then increased significantly by further loading up to 16 h, followed by another decrease up to 1 d of uniaxial stretching. HMSCs subjected to 24 h cyclic uniaxial stretching significantly expressed early and intermediate contractile SM markers. It was hypothesized that the increase in FD after 4 h while cells continuously became more aligned and elongated was due to initiation of change in phenotype that influenced arrangement of cells. At this point, change in cell phenotype started leading to change in morphology while mechanical loading still caused cell alignment and rearrangement. Results can be helpful when optimized engineered cells are needed based on mechanical condition for functional engineered tissue and cell therapy.
authors.affiliation: Cardiovascular Engineering Lab, Faculty of Biomedical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran.
authors.forename: Azim
authors.initials: A
authors.lastname: Parandakh
authors.affiliation: Cardiovascular Engineering Lab, Faculty of Biomedical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran. tafazoli@aut.ac.ir.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Tafazzoli-Shadpour
authors.affiliation: Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. khani@sbmu.ac.ir.|Department of Tissue Engineering and Regenerative Medicine, School of Advanced technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. khani@sbmu.ac.ir.
authors.forename: Mohammad-Mehdi
authors.initials: MM
authors.lastname: Khani
mesh_terms: D008841:Actin Cytoskeleton / Q000648:ultrastructure*; D002454:Cell Differentiation; D003599:Cytoskeleton / Q000235:genetics / Q000648:ultrastructure; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000648:ultrastructure*; D032389:Myocytes, Smooth Muscle / Q000648:ultrastructure*; D013314:Stress, Mechanical; D023822:Tissue Engineering
keywords: Cyclic stretch; Cytoskeletal reorganization; Fractal geometry; Mechanobiology; Morphology