title: Changes of sodium nitrate, nitrite, and N-nitrosodiethylamine during in vitro human digestion.
abstract: This study aimed to determine the changes in sodium nitrate, sodium nitrite, and N-nitrosodiethylamine (NDEA) during in vitro human digestion, and the effect of enterobacteria on the changes in these compounds. The concentrations of nitrate, nitrite, and NDEA were significantly reduced from 150, 150, and 1ppm to 42.8, 63.2, and 0.85ppm, respectively, during in vitro human digestion (p<0.05). The enterobacteria Escherichia coli and Lactobacillus casei reduced the amount of these compounds present during in vitro human digestion. This study is the first to report that E. coli can dramatically reduce the amount of nitrite during in vitro human digestion and this may be due to the effect of nitrite reductase present in E. coli. We therefore conclude that the amounts of potentially harmful substances and their toxicity can be decreased during human digestion.
authors.affiliation: Department of Animal Science and Technology, Chung-Ang University, Anseong-si 17546, Republic of Korea. Electronic address: dock-0307@hanmail.net.
authors.forename: Hyeong Sang
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Animal Science and Technology, Chung-Ang University, Anseong-si 17546, Republic of Korea. Electronic address: hursj@cau.ac.kr.
authors.forename: Sun Jin
authors.initials: SJ
authors.lastname: Hur
mesh_terms: D004052:Diethylnitrosamine / Q000737:chemistry*; D004063:Digestion; D006801:Humans; D009566:Nitrates / Q000737:chemistry*; D009573:Nitrites / Q000737:chemistry*
chemical_list: D009566:Nitrates; D009573:Nitrites; D004052:Diethylnitrosamine; C031618:sodium nitrate
keywords: Carcinogenic substance; Enterobacteria; In vitro human digestion; N-Nitroso compound; Nitrate; Nitrite