title: Mitochondrial dysfunction is involved in the aggravation of periodontitis by diabetes.
abstract: OBJECTIVE
  To elucidate whether mitochondrial dysfunction contributes to aggravated periodontitis in diabetes.
  
  
  METHODS
  Sixty-four wistar rats were randomly assigned into four groups: control, periodontitis, diabetes, and diabetic periodontitis. Two weeks after induction of diabetes, periodontitis was induced by silk ligation for 2 weeks and thereafter evaluated by assessing alveolar bone loss and apoptosis of periodontium cells. Mitochondrial oxidative stress was detected by MitoSOX staining. Mitochondrial function was determined by measuring ATP production, and by assessing mitochondrial DNA copy number, activities of electron transport chain complexes, and biogenesis with real-time PCR.
  
  
  RESULTS
  Significantly severer bone loss, enhanced periodontium cell apoptosis, and mitochondrial oxidative stress were found in the rats with diabetic periodontitis than the others. Furthermore, diabetic rats with periodontitis presented severer mitochondrial dysfunction than lean rats with periodontitis, as reflected by compromised ATP production, decreased mitochondrial DNA copy number, reduced gene expression of electron transport chain complex I subunits, and impaired mitochondrial biogenesis (p < 0.05). Multiple regression analysis further indicated a close correlation between these mitochondrial events and bone loss in diabetic periodontitis.
  
  
  CONCLUSIONS
  Mitochondrial dysfunction was positive correlated to aggravated periodontitis in diabetes and might represent a therapeutic target for diabetic periodontitis.
authors.affiliation: Department of Periodontology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.|Institute of Stomatology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.|Department of Oral Implantology and Prosthetic Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam Movement Sciences, Amsterdam, The Netherlands.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Sun
authors.affiliation: Institute of Stomatology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.|Department of Prosthodontics, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.
authors.forename: Yixin
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Institute of Stomatology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.|Department of Prosthodontics, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.
authors.forename: Panpan
authors.initials: P
authors.lastname: Dai
authors.affiliation: Institute of Stomatology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.|Department of Prosthodontics, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.
authors.forename: Xumin
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Stomatology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.|Department of Prosthodontics, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.
authors.forename: Weiyan
authors.initials: W
authors.lastname: Gu
authors.affiliation: Department of Periodontology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.
authors.forename: Huining
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Oral Implantology and Prosthetic Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam Movement Sciences, Amsterdam, The Netherlands.
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.affiliation: Institute of Stomatology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.|Department of Prosthodontics, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Ma
authors.affiliation: Institute of Stomatology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.|Department of Oral Implantology and Prosthetic Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam Movement Sciences, Amsterdam, The Netherlands.|Department of Prosthodontics, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.
authors.forename: Shengbin
authors.identifier: http://orcid.org/0000-0003-4522-2769
authors.initials: S
authors.lastname: Huang
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis; D016301:Alveolar Bone Loss / Q000209:etiology; D000818:Animals; D017209:Apoptosis; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D056915:DNA Copy Number Variations; D004272:DNA, Mitochondrial / Q000235:genetics; D048909:Diabetes Complications / Q000503:physiopathology*; D003921:Diabetes Mellitus, Experimental / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D008928:Mitochondria / Q000235:genetics / Q000502:physiology*; D001678:Organelle Biogenesis; D018384:Oxidative Stress; D010518:Periodontitis / Q000473:pathology / Q000503:physiopathology*; D012333:RNA, Messenger / Q000378:metabolism; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D004272:DNA, Mitochondrial; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D000255:Adenosine Triphosphate; D013482:Superoxide Dismutase
keywords: diabetes; mitochondrial biogenesis; mitochondrial dysfunction; oxidative stress; periodontal disease(s)