title: Monoterpenoid Indole Alkaloids from Kopsia officinalis and the Immunosuppressive Activity of Rhazinilam.
abstract: Six new monoterpenoid indole alkaloids, kopsinidines C-E (1-3), 11,12-methylenedioxychanofruticosinic acid (4), 12-methoxychanofruticosinic acid (5), and N(4)-methylkopsininate (7), as well as chanofruticosinic acid (6, as a natural product) and 23 known alkaloids, were obtained from the twigs and leaves of Kopsia officinalis. Their structures were characterized by physical data analysis. All isolated compounds were evaluated for their immunosuppressive activity on human T cell proliferation. Rhazinilam (29) significantly inhibited human T cell proliferation activated by anti-CD3/anti-CD28 antibodies (IC50= 1.0 μM) and alloantigen stimulation (IC50= 1.1 μM) without obvious cytotoxicity for naïve human T cells and peripheral blood mononuclear cells (0-320 μM). Although it did not affect T cell activation, it induced T cell cycle arrest in the G2/M phase and inhibited proinflammatory cytokine production in activated T cells.
authors.affiliation: Research Center, Chengdu Medical College , Xindu Avenue 783, Chengdu 610500, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zeng
authors.affiliation: Research Center, Chengdu Medical College , Xindu Avenue 783, Chengdu 610500, People's Republic of China.
authors.forename: Xiu-Yin
authors.initials: XY
authors.lastname: Wu
authors.affiliation: Research Center, Chengdu Medical College , Xindu Avenue 783, Chengdu 610500, People's Republic of China.
authors.forename: Shu-Xia
authors.initials: SX
authors.lastname: Yang
authors.affiliation: Research Center, Chengdu Medical College , Xindu Avenue 783, Chengdu 610500, People's Republic of China.
authors.forename: Wei-Chun
authors.initials: WC
authors.lastname: Lai
authors.affiliation: Research Center, Chengdu Medical College , Xindu Avenue 783, Chengdu 610500, People's Republic of China.
authors.forename: Shun-Dong
authors.initials: SD
authors.lastname: Shi
authors.affiliation: Research Center, Chengdu Medical College , Xindu Avenue 783, Chengdu 610500, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zou
authors.affiliation: Research Center, Chengdu Medical College , Xindu Avenue 783, Chengdu 610500, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Research Center, Chengdu Medical College , Xindu Avenue 783, Chengdu 610500, People's Republic of China.
authors.forename: Li-Mei
authors.identifier: 0000-0002-6681-7414
authors.initials: LM
authors.lastname: Li
mesh_terms: D000470:Alkaloids; D029062:Apocynaceae / Q000737:chemistry*; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D007212:Indolizines; D007769:Lactams; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D046948:Secologanin Tryptamine Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects
chemical_list: D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D007166:Immunosuppressive Agents; D007212:Indolizines; D007769:Lactams; D046948:Secologanin Tryptamine Alkaloids; C000621514:kopsinidine C; C000621512:kopsinidine D; C000621513:kopsinidine E; C009170:rhazinilam