title: Study on the effects of blueberry treatment on histone acetylation modification of CCl4-induced liver disease in rats.
abstract: The objective of this study was to investigate the effects of blueberry treatment on histone acetylation modification of carbon tetrachloride (CCl4)-induced liver disease in rats. Laboratory rats were randomly divided into control, hepatic fibrosis, blueberry treatment, blueberry intervention, and natural recovery groups. Rats in the model groups were treated with CCl4administered subcutaneously at 4- and 8-week intervals, and then executed. Both the 4- and 8-week treatment groups were treated with blueberry juice for 8 weeks, and then executed after 12 and 16 weeks, respectively. Following the experiment, four liver function and hepatic fibrosis indices were measured. Liver index was calculated, hematoxylin-eosin staining was conducted, and H3K9, H3K14, and H3K18 expressions were evaluated among the nuclear proteins of the liver tissues. No differences in alanine transaminase were noted between the control and intervention groups, but significant differences were detected among the model, treatment, and natural recovery groups (P < 0.01). Significant differences were also observed in aspartate transaminase, hyaluronic acid, and collagen IV among the model, treatment, intervention, and natural recovery groups (P < 0.01, P < 0.01, P < 0.01). Liver index, and H3K9 and H3K14 expression were significantly different among the model groups (P < 0.05 and P < 0.01), whereas H3K18 expression was dramatically different among model, treatment, intervention, and natural recovery groups (P < 0.01). Following blueberry treatment, rat liver function and hepatic fibrosis improved, potentially indicating that blueberry components could regulate histone acetylation and improve liver pathologic changes in rats with CCl4-induced disease.
authors.affiliation: Department of General Surgery, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China.
authors.forename: W
authors.initials: W
authors.lastname: Zhan
authors.affiliation: Department of Radiology, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China.
authors.forename: X
authors.initials: X
authors.lastname: Liao
authors.affiliation: Department of Pathophysiology, Guizhou Medical University, Guiyang, Guizhou, China.
authors.forename: T
authors.initials: T
authors.lastname: Tian
authors.affiliation: Department of Pathophysiology, Guizhou Medical University, Guiyang, Guizhou, China.
authors.forename: L
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Pathophysiology, Guizhou Medical University, Guiyang, Guizhou, China.
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Pathology, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China.
authors.forename: B
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Radiology, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China.
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Pathophysiology, Guizhou Medical University, Guiyang, Guizhou, China.
authors.forename: B
authors.initials: B
authors.lastname: Han
authors.affiliation: Department of Pathophysiology, Guizhou Medical University, Guiyang, Guizhou, China.
authors.forename: R J
authors.initials: RJ
authors.lastname: Xie
authors.affiliation: Department of General Surgery, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China yangqin_06l@163.com.
authors.forename: Q H
authors.initials: QH
authors.lastname: Ji
authors.affiliation: Department of Pathophysiology, Guizhou Medical University, Guiyang, Guizhou, China yangqin_06l@163.com.
authors.forename: Q
authors.initials: Q
authors.lastname: Yang
mesh_terms: D000107:Acetylation / Q000187:drug effects; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D029798:Blueberry Plants / Q000737:chemistry*; D002251:Carbon Tetrachloride / Q000633:toxicity*; D004195:Disease Models, Animal; D006657:Histones / Q000378:metabolism*; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D008111:Liver Function Tests; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage* / Q000494:pharmacology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D006657:Histones; D010936:Plant Extracts; D002251:Carbon Tetrachloride; D001219:Aspartate Aminotransferases