title: UHPLC-MS/MS quantification combined with chemometrics for the comparative analysis of different batches of raw and wine-processed Dipsacus asper.
abstract: A rapid and sensitive ultra-high performance liquid chromatography with tandem mass spectrometry approach was established for the simultaneous determination of 4-caffeoylquinic acid, loganic acid, chlorogenic acid, loganin, 3,5-dicaffeoylquinic acid, dipsacoside B, asperosaponin VI, and sweroside in raw and wine-processed Dipsacus asper. Chloramphenicol and glycyrrhetinic acid were employed as internal standards. The proposed approach was fully validated in terms of linearity, sensitivity, precision, repeatability as well as recovery. Intra- and interassay variability for all analytes were 2.8-4.9 and 1.7-4.8%, respectively. The standard addition method determined recovery rates for each analytes (96.8-104.6%). In addition, the developed approach was applied to 20 batches of raw and wine-processed samples of Dipsacus asper. Principle component analysis and partial least squares-discriminate analysis revealed a clear separation between the raw group and wine-processed group. After wine-processing, the contents of loganic acid, chlorogenic acid, dipsacoside B, and asperosaponin VI were upregulated, while the contents of 3,5-dicaffeoylquinic acid, 4-caffeoylquinic acid, loganin, and sweroside were downregulated. Our results demonstrated that ultra-high performance liquid chromatography with tandem mass spectrometry quantification combined with chemometrics is a viable method for quality evaluation of the raw Dipsacus asper and its wine-processed products.
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, P. R. China.|Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, P. R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Tao
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, P. R. China.|Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, P. R. China.
authors.forename: Yingshan
authors.initials: Y
authors.lastname: Du
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, P. R. China.|Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, P. R. China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Su
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, P. R. China.|Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, P. R. China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Li
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, P. R. China.|Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, P. R. China.
authors.forename: Baochang
authors.initials: B
authors.lastname: Cai
mesh_terms: D002851:Chromatography, High Pressure Liquid; D049917:Dipsacaceae / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry; D053719:Tandem Mass Spectrometry; D014920:Wine
chemical_list: D004365:Drugs, Chinese Herbal
keywords: Dipsacus asper; chemometrics; herb processing; traditional Chinese medicine