title: Electrochemical monitoring of biointeraction by graphene-based material modified pencil graphite electrode.
abstract: Recently, the low-cost effective biosensing systems based on advanced nanomaterials have received a key attention for development of novel assays for rapid and sequence-specific nucleic acid detection. The electrochemical biosensor based on reduced graphene oxide (rGO) modified disposable pencil graphite electrodes (PGEs) were developed herein for electrochemical monitoring of DNA, and also for monitoring of biointeraction occurred between anticancer drug, Daunorubicin (DNR), and DNA. First, rGO was synthesized chemically and characterized by using UV-Vis, TGA, FT-IR, Raman Spectroscopy and SEM techniques. Then, the quantity of rGO assembling onto the surface of PGE by passive adsorption was optimized. The electrochemical behavior of rGO-PGEs was examined by cyclic voltammetry (CV). rGO-PGEs were then utilized for electrochemical monitoring of surface-confined interaction between DNR and DNA using differential pulse voltammetry (DPV) technique. Additionally, voltammetric results were complemented with electrochemical impedance spectroscopy (EIS) technique. Electrochemical monitoring of DNR and DNA was resulted with satisfying detection limits 0.55µM and 2.71µg/mL, respectively.
authors.affiliation: Ege University, Faculty of Pharmacy, Analytical Chemistry Department, Bornova 35100 Izmir, Turkey.
authors.forename: Ece
authors.initials: E
authors.lastname: Eksin
authors.affiliation: Necmettin Erbakan University, Faculty of A. K. Education, Science and Technology Department, 42090 Konya, Turkey.
authors.forename: Erhan
authors.initials: E
authors.lastname: Zor
authors.affiliation: Ege University, Faculty of Pharmacy, Analytical Chemistry Department, Bornova 35100 Izmir, Turkey. Electronic address: arzum.erdem@ege.edu.tr.
authors.forename: Arzum
authors.initials: A
authors.lastname: Erdem
authors.affiliation: Necmettin Erbakan University, Faculty of A. K. Education, Chemistry Department, 42090 Konya, Turkey. Electronic address: halukbingol@gmail.com.
authors.forename: Haluk
authors.initials: H
authors.lastname: Bingol
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000378:metabolism*; D003630:Daunorubicin / Q000494:pharmacology*; D058266:Dielectric Spectroscopy; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D005399:Fishes; D006108:Graphite / Q000737:chemistry*; D061925:Immobilized Nucleic Acids / Q000378:metabolism; D008958:Models, Molecular
chemical_list: D000903:Antibiotics, Antineoplastic; D061925:Immobilized Nucleic Acids; D006108:Graphite; D004247:DNA; D003630:Daunorubicin
keywords: Differential pulse voltammetry; Drug-DNA interaction; Electrochemical biosensors; Electrochemical impedance spectroscopy; Pencil graphite electrodes; Reduced graphene oxide