title: Polydatin improves the developmental competence of bovine embryos in vitro via induction of sirtuin 1 (Sirt1).
abstract: The aim of the present study was to investigate the beneficial effect of polydatin (PD), the glycoside form of resveratrol, on embryo development in vitro. Oocytes were aspirated from ovaries of Korean Hanwoo cows and cultured until Day 8 in a humidified atmosphere of 5% CO2in air at 38.5°C. Protein and gene expression levels were determined through confocal microscopy and reverse transcription-polymerase chain reaction respectively, whereas the number of total and apoptotic cells in Day 8 blastocysts was determined using Hoechst 33342 staining and terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling. Of the different concentrations of PD (0.5, 1.0 and 2.0µM) added to the IVM medium, only 1.0µM PD significantly improved blastocyst development. Immunofluorescence analysis confirmed that protein levels of sirtuin 1 (Sirt1) increased significantly (P<0.05) after PD treatment, whereas levels of reactive oxygen species (ROS) were significantly (P<0.05) decreased, as evidenced by reductions in 8-oxoguanine immunoreactivity. Similarly, protein levels of nuclear factor (NF)-κB and cyclo-oxygenase (COX)-2 were significantly (P<0.05) lower in the PD-treated group than in the control group. Treatment with 1.0µM PD reduced gene expression of BCL2-associated X protein, inducible nitric oxide synthase, COX2 and Nfkb, but increased the expression of Sirt1, supporting the immunofluorescence data. PD possesses antioxidant activity and is useful for embryo development in vitro. We conclude that supplementation of IVM medium with PD improves embryo developmental competence via Sirt1.
authors.affiliation: Department of Animal Science, Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju 52828, Gyeongnam Province, Republic of Korea.
authors.forename: Imran
authors.initials: I
authors.lastname: Khan
authors.affiliation: Animal Genetic Resources Research Center, National Institute of Animal Science, Rural Development Administration, Namwon 55717, Republic of Korea.
authors.forename: Sung Woo
authors.initials: SW
authors.lastname: Kim
authors.affiliation: Department of Animal Science, Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju 52828, Gyeongnam Province, Republic of Korea.
authors.forename: Kyung-Lim
authors.initials: KL
authors.lastname: Lee
authors.affiliation: Department of Animal Science, Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju 52828, Gyeongnam Province, Republic of Korea.
authors.forename: Seok-Hwan
authors.initials: SH
authors.lastname: Song
authors.affiliation: Department of Animal Science, Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju 52828, Gyeongnam Province, Republic of Korea.
authors.forename: Ayman
authors.initials: A
authors.lastname: Mesalam
authors.affiliation: Department of Animal Science, Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju 52828, Gyeongnam Province, Republic of Korea.
authors.forename: M M R
authors.initials: MMR
authors.lastname: Chowdhury
authors.affiliation: Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju 52828, Gyeongnam Province, Republic of Korea.
authors.forename: Zia
authors.initials: Z
authors.lastname: Uddin
authors.affiliation: Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Gyeongnam Province, Republic of Korea.
authors.forename: Ki Hun
authors.initials: KH
authors.lastname: Park
authors.affiliation: Department of Animal Science, Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju 52828, Gyeongnam Province, Republic of Korea.
authors.forename: Il-Keun
authors.initials: IK
authors.lastname: Kong
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002417:Cattle; D046149:Embryo Culture Techniques / Q000379:methods / Q000662:veterinary*; D047108:Embryonic Development / Q000187:drug effects* / Q000502:physiology; D005960:Glucosides / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D056564:Sirtuin 1 / Q000378:metabolism*; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D005960:Glucosides; D017382:Reactive Oxygen Species; D013267:Stilbenes; D056564:Sirtuin 1; C058229:polydatin