title: Dinickel-Salphen Complexes as Binders of Human Telomeric Dimeric G-Quadruplexes.
abstract: Three new polyether-tethered dinickel-salphen complexes (2 a-c) have been synthesized and fully characterized by NMR spectroscopy, mass spectrometry, and elemental analyses. The binding affinity and selectivity of these complexes and of the parent mono-nickel complex (1) towards dimeric quadruplex DNA have been determined by UV/Vis titrations, fluorescence spectroscopy, CD spectroscopy, and electrophoresis. These studies have shown that the dinickel-salphen complex with the longest polyether linker (2 c) has higher binding affinity and selectivity towards dimeric quadruplexes (over monomeric quadruplexes) than the dinickel-salphen complexes with the shorter polyether linkers (2 a and 2 b). Complex 2 c also has higher selectivity towards human telomeric dimeric quadruplexes with one TTA linker than the monometallic complex 1. Based on the spectroscopic data, a possible binding mode between complex 2 c and the dimeric G-quadruplex DNA under study is proposed.
authors.affiliation: Department of Chemistry, Imperial College London, South Kensington, London, SW72AZ, UK.|Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, P. R. China.
authors.forename: Chun-Qiong
authors.initials: CQ
authors.lastname: Zhou
authors.affiliation: Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, P. R. China.
authors.forename: Ting-Cong
authors.initials: TC
authors.lastname: Liao
authors.affiliation: Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, P. R. China.
authors.forename: Zi-Qi
authors.initials: ZQ
authors.lastname: Li
authors.affiliation: Department of Chemistry, Imperial College London, South Kensington, London, SW72AZ, UK.
authors.forename: Jorge
authors.initials: J
authors.lastname: Gonzalez-Garcia
authors.affiliation: Department of Chemistry, Imperial College London, South Kensington, London, SW72AZ, UK.
authors.forename: Matthew
authors.initials: M
authors.lastname: Reynolds
authors.affiliation: Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, P. R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Zou
authors.affiliation: Department of Chemistry, Imperial College London, South Kensington, London, SW72AZ, UK.
authors.forename: Ramon
authors.identifier: http://orcid.org/0000-0003-2992-199X
authors.initials: R
authors.lastname: Vilar
mesh_terms: D001483:Base Sequence; D002942:Circular Dichroism; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D019281:Dimerization; D054856:G-Quadruplexes; D006801:Humans; D009532:Nickel / Q000737:chemistry*; D010655:Phenylenediamines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D016615:Telomere / Q000737:chemistry / Q000378:metabolism
chemical_list: D056831:Coordination Complexes; D010655:Phenylenediamines; C545695:salphen; D009532:Nickel
keywords: DNA recognition; dimeric quadruplex DNA; metal salphen; telomere⋅nickel