title: A Dual-Color Quantum Dots Encoded Frit-Based Immunoassay for Visual Detection of Aflatoxin M1and Pirlimycin Residues in Milk.
abstract: Mycotoxins and antibacterial agents are the main chemical hazards that lead to several health problems. Nowadays, multiplex immunoassay is a primary goal throughout the world. Here, aflatoxin M1and pirlimycin were selected as models, and a novel dual colorimetric encoded frit-based immunoassay was developed for simultaneously screening of aflatoxin M1and pirlimycin residues in milk. This multiplex frit-based immunoassay combined two monoclonal antibodies to extend the spectrum of analytes and to enable detection of two classes of analytes in a single test. The cutoff values were 0.02 μg/kg for aflatoxin M1and 0.5 μg/kg for pirlimycin, which satisfied the requirement to measure the maximum residue levels. The novel colorimetric frit-based immunoassay has the advantage of high throughput, short analysis time, reduced overall cost per assay, and can be used as a rapid screening technique for simultaneously detecting aflatoxin M1and pirlimycin residues in milk.
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Shenzhen University Health Sciences Center , Shenzhen 518060, China.
authors.forename: Wenxiao
authors.identifier: http://orcid.org/0000-0003-2443-7648
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Laboratory of Food Analysis, Department of Bioanalysis, Ghent University , Harelbekestraat 72, B-9000 Ghent, Belgium.
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Beloglazova
authors.affiliation: Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center for Food Safety Risk Assessment , Beijing 100021, China.
authors.forename: Pengjie
authors.initials: P
authors.lastname: Luo
authors.affiliation: Technology Center of JiangXi Entry-Exit Inspection and Quarantine Bureau, Nanchang 330038, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Guo
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Shenzhen University Health Sciences Center , Shenzhen 518060, China.
authors.forename: Guimiao
authors.initials: G
authors.lastname: Lin
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Shenzhen University Health Sciences Center , Shenzhen 518060, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Wang
mesh_terms: D016607:Aflatoxin M1 / Q000032:analysis*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002417:Cattle; D002981:Clindamycin / Q000031:analogs & derivatives* / Q000032:analysis; D004350:Drug Residues / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis; D007118:Immunoassay / Q000379:methods*; D008892:Milk / Q000737:chemistry*; D009183:Mycotoxins / Q000032:analysis*; D045663:Quantum Dots / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D009183:Mycotoxins; D002981:Clindamycin; D016607:Aflatoxin M1; C034093:pirlimycin
keywords: aflatoxin M1; food safety; immunoassay; milk; pirlimycin