title: Male reproductive toxicity of CrVI: In-utero exposure to CrVI at the critical window of testis differentiation represses the expression of Sertoli cell tight junction proteins and hormone receptors in adult F1progeny rats.
abstract: The effect of gestational exposure to CrVI (occupational/environmental pollutant and target to Sertoli cells(SC)) was tested in a rat model during the testicular differentiation from the bipotential gonad may interrupt spermatogenesis by disrupting SC tight junctions(TJ) and it's proteins and hormone receptors. Pregnant Wistar rats were exposed to 50/100/200ppm CrVI through drinking water during embryonic days 9-14. On Postnatal day 120, testes were subjected to ion exchange chromatographic analysis and revealed increased level of CrIII in SCs and germ cells, serum and testicular interstitial fluid(TIF). Microscopic analyses showed seminiferous tubules atrophy and disruption of SC TJ, which also recorded decreased testosterone in TIF. mRNA and Protein expression analyses attested decreased level of Fshr, Ar, occludin and claudin-11 in SCs. Immunofluorescent detection revealed weak signal of TJ proteins. Taken together, we concluded that gestational exposure to CrVI interferes with the expression of SC TJ proteins due to attenuated expression of hormone receptors.
authors.affiliation: Department of Endocrinology and Anatomy, Dr. ALM Post-Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600113, India; Department of Clinical and Translational Sciences, Robert C. Byrd Biotech Science Centre, Marshall University, Huntington, WV 25701, USA.
authors.forename: Kathiresh M
authors.initials: KM
authors.lastname: Kumar
authors.affiliation: Department of Endocrinology and Anatomy, Dr. ALM Post-Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600113, India. Electronic address: aruldhasmm@gmail.com.
authors.forename: Mariajoseph Michael
authors.initials: MM
authors.lastname: Aruldhas
authors.affiliation: Department of Endocrinology and Anatomy, Dr. ALM Post-Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600113, India.
authors.forename: Sheerin L
authors.initials: SL
authors.lastname: Banu
authors.affiliation: Department of Endocrinology and Anatomy, Dr. ALM Post-Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600113, India.
authors.forename: Balaji
authors.initials: B
authors.lastname: Sadasivam
authors.affiliation: Department of Endocrinology and Anatomy, Dr. ALM Post-Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600113, India; Department of Otorhinolaryngology, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
authors.forename: Ganapathy
authors.initials: G
authors.lastname: Vengatesh
authors.affiliation: Department of Anatomy, Dr. ALM Post-Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600113, India.
authors.forename: Karthik M
authors.initials: KM
authors.lastname: Ganesh
authors.affiliation: Department of Endocrinology and Anatomy, Dr. ALM Post-Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600113, India.
authors.forename: Shobana
authors.initials: S
authors.lastname: Navaneethabalakrishnan
authors.affiliation: Department of Endocrinology and Anatomy, Dr. ALM Post-Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600113, India.
authors.forename: Ajith Kumar
authors.initials: AK
authors.lastname: Navin
authors.affiliation: Department of Anatomy, Dr. ALM Post-Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600113, India.
authors.forename: Felicia Mary
authors.initials: FM
authors.lastname: Michael
authors.affiliation: Department of Anatomy, Dr. ALM Post-Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600113, India.
authors.forename: Sankar
authors.initials: S
authors.lastname: Venkatachalam
authors.affiliation: Department of Veterinary Integrative Biosciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, Mail Stop: TAMU 4458, College Station, TX 77843, USA.
authors.forename: Jone A
authors.initials: JA
authors.lastname: Stanley
authors.affiliation: Department of Endocrinology and Anatomy, Dr. ALM Post-Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600113, India.
authors.forename: Ilangovan
authors.initials: I
authors.lastname: Ramachandran
authors.affiliation: Department of Veterinary Integrative Biosciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, Mail Stop: TAMU 4458, College Station, TX 77843, USA.
authors.forename: Sakhila K
authors.initials: SK
authors.lastname: Banu
authors.affiliation: Department of Animal Science, Bharathidasan University, Tiruchirappalli 620024, India; Department of Food Science and Nutrition, College of Food Science and Agriculture, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Mohammad Abdulkader
authors.initials: MA
authors.lastname: Akbarsha
mesh_terms: D000818:Animals; D002857:Chromium / Q000097:blood / Q000633:toxicity*; D057167:Claudins / Q000235:genetics / Q000378:metabolism; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D008431:Maternal-Fetal Exchange; D046529:Microscopy, Electron, Transmission; D062793:Occludin / Q000235:genetics / Q000378:metabolism; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D012333:RNA, Messenger / Q000378:metabolism; D017208:Rats, Wistar; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism; D011962:Receptors, FSH / Q000235:genetics / Q000378:metabolism; D013081:Sperm Motility / Q000187:drug effects; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D013739:Testosterone / Q000097:blood; D014874:Water Pollutants, Chemical / Q000097:blood / Q000633:toxicity*
chemical_list: D057167:Claudins; C492353:Cldn11 protein, rat; D062793:Occludin; C568987:Ocln protein, rat; D012333:RNA, Messenger; D011944:Receptors, Androgen; D011962:Receptors, FSH; D014874:Water Pollutants, Chemical; D002857:Chromium; C074702:chromium hexavalent ion; D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone
keywords: Androgen receptor; Claudin; FSH receptor; Male fertility; Occludin