title: Dynamic multimodal full-field optical coherence tomography and fluorescence structured illumination microscopy.
abstract: We report on the development of a configuration of a multimodal full-field optical coherence tomography (FF-OCT) and fluorescence microscope. Our system can simultaneously acquire FF-OCT and structured illumination microscopy images. Dynamic parallel evolution of tissue microstructures and biochemical environments can be visualized. We use high numerical aperture objectives to optimize the combination of the two modalities. We imaged the propagation of mechanical waves initiated by calcium waves in a heart wall to illustrate the interest of simultaneous recording of mechanical and biochemical information.
authors.affiliation: PSL Research University, Institut Langevin, ESPCI Paris, Paris, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Thouvenin
authors.affiliation: PSL Research University, Institut Langevin, ESPCI Paris, Paris, France.
authors.forename: Mathias
authors.initials: M
authors.lastname: Fink
authors.affiliation: PSL Research University, Institut Langevin, ESPCI Paris, Paris, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Boccara
mesh_terms: D002118:Calcium / Q000737:chemistry / Q000378:metabolism*; D006321:Heart / Q000000981:diagnostic imaging*; D006801:Humans; D008856:Microscopy, Fluorescence; D064847:Multimodal Imaging; D041623:Tomography, Optical Coherence
chemical_list: D002118:Calcium