title: Mesenchymal stem cell-derived CCN2 promotes the proliferation, migration and invasion of human tongue squamous cell carcinoma cells.
abstract: Recent studies have demonstrated that mesenchymal stem cells (MSC) exhibit a tropism to tumors and form the tumor stroma. In addition, we found that MSC can secrete different types of factors. However, the involvement of MSC-derived factors in human tongue squamous cell carcinoma (TSCC) growth has not been clearly addressed. The CCN family includes multifunctional signaling molecules that affect the initiation and development events of various tumors. In our study, we report that CCN2/connective tissue growth factor (CTGF) was the most highly induced among the CCN family members in MSC that were co-cultured with TSCC cells. To evaluate the relationship between CCN2 and TSCC growth, we downregulated MSC-derived CCN2 expression with shRNA targeting CCN2 and found that MSC-secreted CCN2 promotes TSCC cell proliferation, migration and invasion. We also confirmed that MSC-derived CCN2 partially accelerated tumor growth in vitro. Taken together, these results suggest that MSC-derived CCN2 contributes to the promotion of proliferation, migration and invasion of TSCC cells and may be a possible therapy target in the future.
authors.affiliation: Department of Oral & Maxillofacial Surgery, Sun Yat-sen Memorial Hospital, Guangzhou, China.
authors.forename: Yu-Ling
authors.identifier: http://orcid.org/0000-0003-0909-7470
authors.initials: YL
authors.lastname: Wu
authors.affiliation: Department of Oral & Maxillofacial Surgery, Sun Yat-sen Memorial Hospital, Guangzhou, China.|Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Li
authors.affiliation: Department of Oral & Maxillofacial Surgery, Sun Yat-sen Memorial Hospital, Guangzhou, China.
authors.forename: Xiao-Peng
authors.initials: XP
authors.lastname: Zhao
authors.affiliation: Department of Oral & Maxillofacial Surgery, Sun Yat-sen Memorial Hospital, Guangzhou, China.
authors.forename: Jiu-Yang
authors.initials: JY
authors.lastname: Jiao
authors.affiliation: Department of Oral & Maxillofacial Surgery, Sun Yat-sen Memorial Hospital, Guangzhou, China.
authors.forename: Dong-Xiao
authors.initials: DX
authors.lastname: Tang
authors.affiliation: Department of Oral & Maxillofacial Surgery, Sun Yat-sen Memorial Hospital, Guangzhou, China.
authors.forename: Ling-Jian
authors.initials: LJ
authors.lastname: Yan
authors.affiliation: Department of Oral & Maxillofacial Surgery, Sun Yat-sen Memorial Hospital, Guangzhou, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Wan
authors.affiliation: Department of Oral & Maxillofacial Surgery, Sun Yat-sen Memorial Hospital, Guangzhou, China.
authors.forename: Chao-Bin
authors.initials: CB
authors.lastname: Pan
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D055513:Connective Tissue Growth Factor / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000235:genetics* / Q000473:pathology; D014062:Tongue Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C526317:CCN2 protein, human; D055513:Connective Tissue Growth Factor
keywords: CCN2; mesenchymal stem cells; migration; proliferation; tongue squamous cell carcinoma