title: Effect of alprazolam on rat serum metabolic profiles.
abstract: We developed a serum metabolomic method by gas chromatography-mass spectrometry (GC-MS) to evaluate the effect of alprazolam in rats. The GC-MS with HP-5MS (0.25 μm × 30 m × 0.25 mm) mass was conducted in electron impact ionization (EI) mode with electron energy of 70 eV, and full-scan mode with m/z 50-550. The rats were randomly divided to four groups, three alprazolam-treated groups and a control group. The alprazolam-treated rats were given 5, 10 or 20 mg/kg (low, medium, high) of alprazolam by intragastric administration each day for 14 days. The serum samples were corrected on the seventh and fourteenth days for metabolomic study. The blood was collected for biochemical tests. Then liver and brain were rapidly isolated and immersed for pathological study. Compared with the control group, on the seventh and fourteen days, the levels of d-glucose, 9,12-octadecadienoic acid, butanoic acid, l-proline, d-mannose and malic acid had changed, indicating that alprazolam induced energy metabolism, fatty acid metabolism and amino acid metabolism perturbations in rats. There was no significant difference for alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, urea and uric acid between controls and alprazolam groups. According to the pathological results, alprazolam is not hepatotoxic. Metabolomics could distinguish different alprazolam doses in rats.
authors.affiliation: Department of Neurology, the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: The Department of Pharmacy, Taizhou Cancer Hospital, Wenling, China.
authors.forename: Gaotong
authors.initials: G
authors.lastname: Lin
authors.affiliation: Analytical and Testing Center, Wenzhou Medical University, Wenzhou, China.
authors.forename: Bingbao
authors.initials: B
authors.lastname: Chen
authors.affiliation: Analytical and Testing Center, Wenzhou Medical University, Wenzhou, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Medicine, Shandong University, Jinan, China.
authors.forename: Lingtian
authors.identifier: http://orcid.org/0000-0002-9821-3770
authors.initials: L
authors.lastname: Wang
authors.affiliation: Analytical and Testing Center, Wenzhou Medical University, Wenzhou, China.
authors.forename: Zixia
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Neurology, the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
authors.forename: Yungang
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Analytical and Testing Center, Wenzhou Medical University, Wenzhou, China.
authors.forename: Congcong
authors.initials: C
authors.lastname: Wen
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
authors.forename: Xuezhi
authors.initials: X
authors.lastname: Yang
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
authors.forename: Gaozhong
authors.initials: G
authors.lastname: Cao
authors.affiliation: Analytical and Testing Center, Wenzhou Medical University, Wenzhou, China.
authors.forename: Xianqin
authors.initials: X
authors.lastname: Wang
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
authors.forename: Guoquan
authors.initials: G
authors.lastname: Cao
mesh_terms: D000525:Alprazolam / Q000494:pharmacology*; D000596:Amino Acids / Q000032:analysis / Q000097:blood / Q000378:metabolism; D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D005947:Glucose / Q000032:analysis / Q000378:metabolism; D019787:Linoleic Acid / Q000032:analysis / Q000097:blood / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008358:Mannose / Q000032:analysis / Q000097:blood / Q000378:metabolism; D055442:Metabolome / Q000187:drug effects*; D055432:Metabolomics; D025341:Principal Component Analysis; D051381:Rats
chemical_list: D000596:Amino Acids; D019787:Linoleic Acid; D005947:Glucose; D008358:Mannose; D000525:Alprazolam
keywords: GC-MS; alprazolam; metabolomics; rat; serum