title: Clinical and Molecular Evidence of ABCC11 Protein Expression in Axillary Apocrine Glands of Patients with Axillary Osmidrosis.
abstract: Accumulating evidence suggests that the risk of axillary osmidrosis is governed by a non-synonymous single nucleotide polymorphism (SNP) 538G>A in humanATP-binding cassette C11(ABCC11) gene. However, little data are available for the expression of ABCC11 protein in human axillary apocrine glands that produce apocrine sweat-a source of odor from the armpits. To determine the effect of the non-synonymous SNPABCC11538G>A (G180R) on the ABCC11 in vivo, we generated transiently ABCC11-expressing transgenic mice with adenovirus vector, and examined the protein levels of each ABCC11 in the mice with immunoblotting using an anti-ABCC11 antibody we have generated in the present study. Furthermore, we examined the expression of ABCC11 protein in human axillary apocrine glands extracted from axillary osmidrosis patients carrying eachABCC11genotype: 538GG, GA, and AA. Analyses of transiently ABCC11-expressing transgenic mice showed thatABCC11538G>A diminishes the ABCC11 protein levels in vivo. Consistently, ABCC11 protein was detected in the human axillary apocrine glands of the 538GG homozygote or 538GA heterozygote, not in the 538AA homozygote. These findings would contribute to a better understanding of the molecular basis of axillary osmidrosis.
authors.affiliation: Department of Pharmacy, The University of Tokyo Hospital, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan. ytoyoda-tky@umin.ac.jp.
authors.forename: Yu
authors.initials: Y
authors.lastname: Toyoda
authors.affiliation: Department of Pharmacy, The University of Tokyo Hospital, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan. tappei-tky@umin.ac.jp.
authors.forename: Tappei
authors.initials: T
authors.lastname: Takada
authors.affiliation: Gomi Clinic, 1-10-12, Hyakunin-cho, Shinjyuku-ku, Tokyo 169-0073, Japan. gomi@gomiclinic.com.
authors.forename: Tsuneaki
authors.initials: T
authors.lastname: Gomi
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai 487-8501, Japan. hnakagaw@isc.chubu.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakagawa
authors.affiliation: RIKEN Center for Life Science Technology, 1-7-22, Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan. toshihisa.ishikawa.r@gmail.com.
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Ishikawa
authors.affiliation: Department of Pharmacy, The University of Tokyo Hospital, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan. suzukihi-tky@umin.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Suzuki
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001050:Apocrine Glands / Q000378:metabolism*; D001365:Axilla; D004195:Disease Models, Animal; D015870:Gene Expression; D005838:Genotype; D006801:Humans; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D020641:Polymorphism, Single Nucleotide; D013543:Sweat Gland Diseases / Q000235:genetics / Q000378:metabolism*
chemical_list: C435500:ABCC11 protein, human; D018528:ATP-Binding Cassette Transporters
keywords: MRP8; armpit; body odor; protein stability; single nucleotide polymorphism; transporter