title: Mitochondrial genotype modulates mtDNA copy number and organismal phenotype in Drosophila.
abstract: We evaluated the role of natural mitochondrial DNA (mtDNA) variation on mtDNA copy number, biochemical features and life history traits in Drosophila cybrid strains. We demonstrate the effects of both coding region and non-coding A+T region variation on mtDNA copy number, and demonstrate that copy number correlates with mitochondrial biochemistry and metabolically important traits such as development time. For example, high mtDNA copy number correlates with longer development times. Our findings support the hypothesis that mtDNA copy number is modulated by mtDNA genome variation and suggest that it affects OXPHOS efficiency through changes in the organization of the respiratory membrane complexes to influence organismal phenotype.
authors.affiliation: Institute of Biosciences and Medical Technology, FI-331014, University of Tampere, Finland.
authors.forename: Tiina S
authors.initials: TS
authors.lastname: Salminen
authors.affiliation: Institute of Biosciences and Medical Technology, FI-331014, University of Tampere, Finland; Department of Biochemistry and Molecular Biology, Center for Mitochondrial Science and Medicine, Michigan State University, East Lansing, MI 48824, USA; Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista "Júlio de Mesquita Filho", Jaboticabal, SP 14884-900, Brazil.
authors.forename: Marcos T
authors.initials: MT
authors.lastname: Oliveira
authors.affiliation: Institute of Biosciences and Medical Technology, FI-331014, University of Tampere, Finland.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Cannino
authors.affiliation: Institute of Biosciences and Medical Technology, FI-331014, University of Tampere, Finland.
authors.forename: Päivi
authors.initials: P
authors.lastname: Lillsunde
authors.affiliation: Institute of Biosciences and Medical Technology, FI-331014, University of Tampere, Finland; Institute of Biotechnology, FI-00014, University of Helsinki, Helsinki, Finland.
authors.forename: Howard T
authors.initials: HT
authors.lastname: Jacobs
authors.affiliation: Institute of Biosciences and Medical Technology, FI-331014, University of Tampere, Finland; Department of Biochemistry and Molecular Biology, Center for Mitochondrial Science and Medicine, Michigan State University, East Lansing, MI 48824, USA. Electronic address: lskaguni@msu.edu.
authors.forename: Laurie S
authors.initials: LS
authors.lastname: Kaguni
mesh_terms: D000818:Animals; D019069:Cell Respiration; D004272:DNA, Mitochondrial / Q000235:genetics*; D004330:Drosophila / Q000235:genetics* / Q000502:physiology*; D005260:Female; D018628:Gene Dosage; D005838:Genotype; D008297:Male; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D010085:Oxidative Phosphorylation; D010641:Phenotype
chemical_list: D004272:DNA, Mitochondrial
keywords: Cybrid; Haplotype; Mitochondrial DNA; Respiration; Supercomplexes