title: Calibration Curve for Dicentric Chromosomes Induced in Human Blood Lymphocytes Exposed to Gamma Rays at a Dose Rate of 12.5 mGy/s.
abstract: To develop a calibration curve for induction of dicentric chromosomes by radiation, we have used a 60Co gamma-ray source with dose rate of 12.5 mGy/s. Whole blood from 15 healthy donors was collected. Whole blood from each donor was divided equally into 8 parts for exposing to supposedly physical doses 0, 0.30, 0.50, 1.00, 1.50, 2.00, 3.00 and 4.00 Gy for a independent calibration curve. Whole blood from 15 donors was used to calibrate dose - effect and statistical for general calibration curve. Using Poisson test (U-test) for the distribution of dicentric chromosomes in the metaphases to determine the uniformity of the radiation field. The average from 15 independent calibration curves of linear correlated coefficient was determined to ber(y, d) = 0.5136 ± 0.0038. The model equation derived is y = aD + bD2+ C. The calibration equation of dose-effect was y = 1.01D + 4.43D2+ 0.56.
authors.affiliation: Department of Biotechnology, Biodosimetry Section, Nuclear Research Institute, Vietnam Atomic Energy Commission, VINATOM, Dalat, Vietnam.
authors.forename: Tran
authors.initials: T
authors.lastname: Que
authors.affiliation: Department of Biotechnology, Biodosimetry Section, Nuclear Research Institute, Vietnam Atomic Energy Commission, VINATOM, Dalat, Vietnam.
authors.forename: Pham Ngoc
authors.initials: PN
authors.lastname: Duy
authors.affiliation: Department of Biotechnology, Biodosimetry Section, Nuclear Research Institute, Vietnam Atomic Energy Commission, VINATOM, Dalat, Vietnam.
authors.forename: Bui Thi Kim
authors.initials: BT
authors.lastname: Luyen
keywords: Exposed dose point; gamma rays; general curve; independent curve; uniform radiation field