title: Results from a round robin test for the ecotoxicological evaluation of construction products using two leaching tests and an aquatic test battery.
abstract: A European round robin test according to ISO 5725-2 was conceptually prepared, realised, and evaluated. The aim was to determine the inter-laboratory variability of the overall process for the ecotoxicological characterization of construction products in eluates and bioassays. To this end, two construction products BAM-G1 (granulate) and HSR-2 (roof sealing sheet), both made of EPDM polymers (rubber), were selected. The granular construction product was eluted in a one stage batch test, the planar product in the Dynamic Surface Leaching test (DSLT). A total of 17 laboratories from 5 countries participated in the round robin test: Germany (12), Austria (2), Belgium (1), Czech Republic (1) and France (1). A test battery of four standardised ecotoxicity tests with algae, daphnia, luminescent bacteria and zebrafish eggs was used. As toxicity measures, EC50 and LID values were calculated. All tests, except the fish egg test, were basically able to demonstrate toxic effects and the level of toxicity. The reproducibility of test results depended on the test specimens and the test organisms. Generally, the variability of the EC50 or LID values increased with the overall level of toxicity. For the very toxic BAM-G1 eluate a relative high variability of CV = 73%-110% was observed for EC50 in all biotests, while for the less toxic HSR-2 eluate the reproducibility of EC50 varied with sensitivity: it was very good (CV = 9.3%) for the daphnia test with the lowest sensitivity, followed by the algae test (CV = 36.4%). The luminescent bacteria test, being the most sensitive bioassay for HSR-2 Eluate, showed the highest variability (CV = 74.8%). When considering the complex overall process the reproducibility of bioassays with eluates from construction products was acceptable.
authors.affiliation: Hydrotox GmbH, Freiburg, Germany. Electronic address: gartiser@hydrotox.de.
authors.forename: Stefan
authors.initials: S
authors.lastname: Gartiser
authors.affiliation: Hydrotox GmbH, Freiburg, Germany.
authors.forename: Ines
authors.initials: I
authors.lastname: Heisterkamp
authors.affiliation: BAM Federal Institute for Materials Research and Testing, Berlin, Germany.
authors.forename: Ute
authors.initials: U
authors.lastname: Schoknecht
authors.affiliation: HSR University of Applied Sciences Rapperswil, Institute for Environmental and Process Engineering (UMTEC), Rapperswil, Switzerland.
authors.forename: Michael
authors.initials: M
authors.lastname: Burkhardt
authors.affiliation: ToxRat Solutions GmbH, Alsdorf, Germany.
authors.forename: Monika
authors.initials: M
authors.lastname: Ratte
authors.affiliation: Federal Environment Agency, Dessau, Germany.
authors.forename: Outi
authors.initials: O
authors.lastname: Ilvonen
authors.affiliation: Federal Environment Agency FG III 2.5, Berlin, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Brauer
authors.affiliation: Institut Dr. Nowak, Ottersberg, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Brückmann
authors.affiliation: Eurofins Agroscience Services Ecotox GmbH, Niefern-Öschelbronn, Germany.
authors.forename: André
authors.initials: A
authors.lastname: Dabrunz
authors.affiliation: ECT Oekotoxikologie GmbH, Flörsheim/Main, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Egeler
authors.affiliation: Lenzing AG - Safety, Health and Environment Department, Lenzing, Austria.
authors.forename: Andrea-Maria
authors.initials: AM
authors.lastname: Eisl
authors.affiliation: Federal Institute of Hydrology, Koblenz, Germany.
authors.forename: Ute
authors.initials: U
authors.lastname: Feiler
authors.affiliation: University of Natural Resources and Life Sciences Vienna - IFA-Tulln, Austria.
authors.forename: Ines
authors.initials: I
authors.lastname: Fritz
authors.affiliation: WESSLING GmbH, Altenberge, Germany.
authors.forename: Sabina
authors.initials: S
authors.lastname: König
authors.affiliation: SGS Institut Fresenius GmbH, Taunusstein, Germany.
authors.forename: Herbert
authors.initials: H
authors.lastname: Lebertz
authors.affiliation: INERIS, Verneuil en Halatte, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Pandard
authors.affiliation: IDUS - Biologisch Analytisches Umweltlabor, Ottendorf-Okrilla, Germany.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Pötschke
authors.affiliation: Dr. U.Noack-Laboratorien, Sarstedt, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Scheerbaum
authors.affiliation: Niedersächsischer Landesbetrieb für Wasserwirtschaft, Küsten- und Naturschutz, Hildesheim, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Schreiber
authors.affiliation: Masaryk Water Research Institute, Ostrava, Czechia.
authors.forename: Přemysl
authors.initials: P
authors.lastname: Soldán
authors.affiliation: Hygiene-Institut des Ruhrgebiets - Institute for Environmental Hygiene and Toxicology, Gelsenkirchen, Germany.
authors.forename: Roland
authors.initials: R
authors.lastname: Weiß
authors.affiliation: VITO Environmental Toxicology, Mol, Belgium.
authors.forename: Reinhilde
authors.initials: R
authors.lastname: Weltens
mesh_terms: D000818:Animals; D001419:Bacteria / Q000187:drug effects; D001681:Biological Assay / Q000379:methods / Q000592:standards; D003621:Daphnia / Q000187:drug effects; D054750:Ecotoxicology / Q000379:methods* / Q000592:standards; D004531:Eggs; D020724:Elastomers / Q000633:toxicity; D005030:Ethylenes / Q000633:toxicity; D015588:Observer Variation; D015203:Reproducibility of Results; D012408:Rubber / Q000633:toxicity; D058009:Stramenopiles / Q000187:drug effects; D018675:Toxicity Tests / Q000379:methods* / Q000592:standards; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*; D015027:Zebrafish
chemical_list: D020724:Elastomers; D005030:Ethylenes; D014874:Water Pollutants, Chemical; C505585:ethylene-propylene-diene-monomer; D012408:Rubber
keywords: Construction products; Ecotoxicity tests; Eluates; Leaching tests; Round robin test