title: Tailoring Cellular Uptake and Fluorescence of Poly(2-oxazoline)-Based Nanogels.
abstract: Controlling the size and charge of nanometer-sized objects is of upmost importance for their interactions with cells. We herein present the synthesis of poly(2-oxazoline) based nanogels comprising a hydrophilic shell and an amine containing core compartment. Amine groups were cross-linked using glutaraldehyde resulting in imine based nanogels. As a drug model, amino fluorescein was covalently immobilized within the core, quenching excessive aldehyde functions. By varying the amount of cross-linker, the zeta potential and, hence, the cellular uptake could be adjusted. The fluorescence of the nanogels was found to be dependent on the cross-linking density. Finally, the hemocompatibility of the described systems was studied by hemolysis and erythrocyte aggregation assays. While cellular uptake was shown to be dependent on the zeta potential of the nanogel, no harmful effects to red blood cells was observed, rendering the present system as an interesting toolbox for the production of nanomaterials with a defined biological interaction profile.
authors.affiliation: Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena , Humboldtstrasse 10, 07743, Jena, Germany.|Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena , Philosophenweg 7, 07743, Jena, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Hartlieb
authors.affiliation: Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena , Humboldtstrasse 10, 07743, Jena, Germany.|Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena , Philosophenweg 7, 07743, Jena, Germany.
authors.forename: Tanja
authors.initials: T
authors.lastname: Bus
authors.affiliation: Institute of Physical Chemistry (IPC) and Abbe Center of Photonics, Friedrich Schiller University Jena , Helmholtzweg 4, 07743 Jena, Germany.|Leibniz Institute of Photonic Technology (IPHT) , Albert-Einstein-Str. 9, 07745 Jena, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Kübel
authors.affiliation: Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena , Humboldtstrasse 10, 07743, Jena, Germany.|Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena , Philosophenweg 7, 07743, Jena, Germany.
authors.forename: David
authors.initials: D
authors.lastname: Pretzel
authors.affiliation: Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena , Humboldtstrasse 10, 07743, Jena, Germany.|Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena , Philosophenweg 7, 07743, Jena, Germany.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Hoeppener
authors.affiliation: Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena , Humboldtstrasse 10, 07743, Jena, Germany.|Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena , Philosophenweg 7, 07743, Jena, Germany.
authors.forename: Meike N
authors.initials: MN
authors.lastname: Leiske
authors.affiliation: Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena , Humboldtstrasse 10, 07743, Jena, Germany.|Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena , Philosophenweg 7, 07743, Jena, Germany.
authors.forename: Kristian
authors.initials: K
authors.lastname: Kempe
authors.affiliation: Institute of Physical Chemistry (IPC) and Abbe Center of Photonics, Friedrich Schiller University Jena , Helmholtzweg 4, 07743 Jena, Germany.|Leibniz Institute of Photonic Technology (IPHT) , Albert-Einstein-Str. 9, 07745 Jena, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Dietzek
authors.affiliation: Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena , Humboldtstrasse 10, 07743, Jena, Germany.|Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena , Philosophenweg 7, 07743, Jena, Germany.
authors.forename: Ulrich S
authors.identifier: 0000-0003-4978-4670
authors.initials: US
authors.lastname: Schubert
mesh_terms: D000588:Amines; D000818:Animals; D002460:Cell Line; D004912:Erythrocytes / Q000378:metabolism; D005453:Fluorescence; D006801:Humans; D000080385:Nanogels; D010080:Oxazoles / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000493:pharmacokinetics / Q000633:toxicity; D011094:Polyethyleneimine / Q000737:chemistry* / Q000493:pharmacokinetics / Q000633:toxicity
chemical_list: D000588:Amines; D000080385:Nanogels; D010080:Oxazoles; C577913:poly(2-oxazoline); C413692:polyethylene glycol polyethyleneimine nanogel; D011092:Polyethylene Glycols; D011094:Polyethyleneimine