title: Polyphenols rich Passiflora leschenaultii leaves modulating Farnesoid X Receptor and Pregnane X Receptor against paracetamol-induced hepatotoxicity in rats.
abstract: The hepatoprotective effect of P. leschenaultii. (DC) leaves was investigated in rats under paracetamol induced oxidative stress. Leaf acetone extract (200 and 400mg/kg) were administered daily via gavage for 14days before paracetamol (2000mg/kg, p.o.) treatment. After the experiment, the levels of serum biochemical parameters and enzymatic antioxidant levels were determined. Furthermore, liver tissues were analyzed histopathologically. Additionally, the molecular docking studies of the identified compounds against PXR and FXR proteins were also performed. The assessment revealed that the acetone extract significantly reduced the elevated levels of SGPT, SGOT and ALP in serum. Moreover, the enzymatic antioxidants such as SOD, CAT and LPO were also retained normally by the plant extract. From histopathological analysis, it was clearly evident that the cellular architecture of plant extract treated rat liver tissues were not affected by the paracetamol induction at the higher dose. The results of docking studies also revealed that the identified compounds showed steric interactions (between nonpolar atoms) with amino acid groups. Collectively, the present study suggests that P. leschenaultii leaves extract protects the liver from paracetamol induced hepatic damage.
authors.affiliation: Department of Pharmacy, Federal University of Sergipe, CEP, 49100-000, Sao Cristovao, Brazil. Electronic address: saranflora04@gmail.com.
authors.forename: Saravanan
authors.initials: S
authors.lastname: Shanmugam
authors.affiliation: Bioprospecting laboratory, Department of Botany, Bharathiar University, Coimbatore, 641 046, Tamil Nadu, India.
authors.forename: Dhivya
authors.initials: D
authors.lastname: Sivaraj
authors.affiliation: Department of Pharmacy, Federal University of Sergipe, CEP, 49100-000, Sao Cristovao, Brazil.
authors.forename: Bruno
authors.initials: B
authors.lastname: Dos Santos Lima
authors.affiliation: Department of Pharmacy, Federal University of Sergipe, CEP, 49100-000, Sao Cristovao, Brazil.
authors.forename: Paula
authors.initials: P
authors.lastname: Dos Passos Menezes
authors.affiliation: Department of Pharmacy, Federal University of Sergipe, CEP, 49100-000, Sao Cristovao, Brazil.
authors.forename: Yasmim Maria Barbosa Gomes
authors.initials: YMBG
authors.lastname: de Carvalho
authors.affiliation: Department of Pharmacy, Federal University of Sergipe, CEP, 49100-000, Sao Cristovao, Brazil. Electronic address: adriasa2001@yahoo.com.br.
authors.forename: Adriano Antunes
authors.initials: AA
authors.lastname: de Souza Araújo
authors.affiliation: Laboratory of Flavor and Chromatographic Analysis, Federal University of Sergipe, CEP, 49100-000, Sao Cristovao, Brazil.
authors.forename: Narendra
authors.initials: N
authors.lastname: Narain
authors.affiliation: Department of Pharmacy, Federal University of Sergipe, CEP, 49100-000, Sao Cristovao, Brazil.
authors.forename: Mairim Russo
authors.initials: MR
authors.lastname: Serafini
authors.affiliation: Department of Pharmacy, Federal University of Sergipe, CEP, 49100-000, Sao Cristovao, Brazil.
authors.forename: Lucindo José
authors.initials: LJ
authors.lastname: Quintans Júnior
authors.affiliation: Department of Chemistry, Federal University of Paraíba, Joao Pessoa, Paraíba, CEP, 58051900, Brazil.
authors.forename: Luciana
authors.initials: L
authors.lastname: Scotti
authors.affiliation: Department of Chemistry, Federal University of Paraíba, Joao Pessoa, Paraíba, CEP, 58051900, Brazil.
authors.forename: Marcus Tulius
authors.initials: MT
authors.lastname: Scotti
authors.affiliation: Bioprospecting laboratory, Department of Botany, Bharathiar University, Coimbatore, 641 046, Tamil Nadu, India. Electronic address: drparimel@gmail.com.
authors.forename: Thangaraj
authors.initials: T
authors.lastname: Parimelazhagan
mesh_terms: D000082:Acetaminophen / Q000009:adverse effects*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D001835:Body Weight / Q000187:drug effects; D008107:Liver Diseases / Q000097:blood / Q000188:drug therapy* / Q000201:enzymology; D008297:Male; D062105:Molecular Docking Simulation; D009929:Organ Size / Q000187:drug effects; D029598:Passiflora / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry / Q000627:therapeutic use*; D000077297:Pregnane X Receptor; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use; D017208:Rats, Wistar; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D012838:Silymarin / Q000494:pharmacology / Q000627:therapeutic use; D000099150:Receptor, Farnesoid X-Activated
chemical_list: D000082:Acetaminophen; D000975:Antioxidants; D015415:Biomarkers; D010936:Plant Extracts; D059808:Polyphenols; D000077297:Pregnane X Receptor; D020011:Protective Agents; D018160:Receptors, Cytoplasmic and Nuclear; D011987:Receptors, Steroid; D012838:Silymarin; D000099150:Receptor, Farnesoid X-Activated
keywords: Caffeic acid; FXR; Hepatoprotective; PXR; Paracetamol; Rutin