title: Use of dual priming oligonucleotide system-based multiplex RT-PCR combined with high performance liquid chromatography assay for simultaneous detection of five enteric viruses associated with acute enteritis.
abstract: In this study, a specific and sensitive method for simultaneous detection of human astrovirus, human rotavirus, norovirus, sapovirus and enteric adenovirus associated with acute enteritis was developed, based on the specific dual priming oligonucleotide (DPO) system and the sensitive high-performance liquid chromatography (HPLC) analysis. The DPO system-based multiplex reverse transcription-polymerase chain reaction (RT-PCR) combined with HPLC assay was more sensitive than agarose gel electrophoresis analysis and real-time SYBR Green PCR assay, and showed a specificity of 100% and sensitivity of 96%-100%. The high sensitivity and specificity of the assay indicates its great potential to be a useful tool for the accurate diagnosis of enteric virus infections.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, PR China.
authors.forename: Wen-Lu
authors.initials: WL
authors.lastname: Fan
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, PR China.
authors.forename: Zi-Wei
authors.initials: ZW
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Qin
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Sun
authors.affiliation: Technology Center, Heilongjiang Entry-Exit Inspection and Quarantine Bureau, Harbin, PR China.
authors.forename: Zhong-Mei
authors.initials: ZM
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, PR China.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Jiang
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, PR China.
authors.forename: Xin-Yuan
authors.initials: XY
authors.lastname: Qiao
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, PR China.
authors.forename: Li-Jie
authors.initials: LJ
authors.lastname: Tang
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, PR China.
authors.forename: Yi-Jing
authors.initials: YJ
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, PR China. Electronic address: yigangxu_china@sohu.com.
authors.forename: Yi-Gang
authors.initials: YG
authors.lastname: Xu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004751:Enteritis / Q000175:diagnosis*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014777:Virus Diseases / Q000175:diagnosis*
keywords: Accurate diagnosis; DPO system-based mRT-PCR-HPLC assay; Five enteric viruses associated with acute enteritis