title: Optimal Fermentation Conditions of Hyaluronidase Inhibition Activity onAsparagus cochinchinensisMerrill byWeissella cibaria.
abstract: This study was conducted to evaluate the hyaluronidase (HAase) inhibition activity ofAsparagus cochinchinesis(AC) extracts following fermentation byWeissella cibariathrough response surface methodology. To optimize the HAase inhibition activity, a central composite design was introduced based on four variables: the concentration of AC extract (X1: 1-5%), amount of starter culture (X2: 1-5%), pH (X3: 4-8), and fermentation time (X4: 0-10 days). The experimental data were fitted to quadratic regression equations, the accuracy of the equations was analyzed by ANOVA, and the regression coefficients for the surface quadratic model of HAase inhibition activity in the fermented AC extract were estimated by theFtest and the correspondingpvalues. The HAase inhibition activity indicated that fermentation time was most significant among the parameters within the conditions tested. To validate the model, two different conditions among those generated by the Design Expert program were selected. Under both conditions, predicted and experimental data agreed well. Moreover, the content of protodioscin (a well-known compound related to anti-inflammation activity) was elevated after fermentation of the AC extract at the optimized fermentation condition.
authors.affiliation: Department of Food Science and Nutrition, College of Human Ecology, Pusan National University, Busan 46241, Republic of Korea.
authors.forename: Minji
authors.initials: M
authors.lastname: Kim
authors.affiliation: Department of Food Science and Nutrition, College of Human Ecology, Pusan National University, Busan 46241, Republic of Korea.
authors.forename: Won-Baek
authors.initials: WB
authors.lastname: Kim
authors.affiliation: Department of Food Science and Nutrition, College of Human Ecology, Pusan National University, Busan 46241, Republic of Korea.
authors.forename: Kyoung Yoon
authors.initials: KY
authors.lastname: Koo
authors.affiliation: Department of Food Science and Nutrition, College of Human Ecology, Pusan National University, Busan 46241, Republic of Korea.
authors.forename: Bo Ram
authors.initials: BR
authors.lastname: Kim
authors.affiliation: Department of Food Science and Nutrition, College of Human Ecology, Pusan National University, Busan 46241, Republic of Korea.
authors.forename: Doohyun
authors.initials: D
authors.lastname: Kim
authors.affiliation: Department of Food Science and Nutrition, College of Human Ecology, Pusan National University, Busan 46241, Republic of Korea.
authors.forename: Seoyoun
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Life Science and Environment Biochemistry, College of Natural Resource and Life Sciences, Pusan National University, Gyeongsangnam-do 50463, Republic of Korea.
authors.forename: Hong Joo
authors.initials: HJ
authors.lastname: Son
authors.affiliation: Department of Biomaterials Science, College of Natural Resource and Life Sciences, Pusan National University, Gyeongsangnam-do 50463, Republic of Korea.
authors.forename: Dae Youn
authors.initials: DY
authors.lastname: Hwang
authors.affiliation: Department of Food Science and Technology, College of Natural Resource and Life Sciences, Pusan National University, Gyeongsangnam-do 50463, Republic of Korea.
authors.forename: Dong Seob
authors.initials: DS
authors.lastname: Kim
authors.affiliation: Gangrim Organics, Gyeongsangnam-do 50455, Republic of Korea.
authors.forename: Chung Yeoul
authors.initials: CY
authors.lastname: Lee
authors.affiliation: Department of Food Science and Nutrition, College of Human Ecology, Pusan National University, Busan 46241, Republic of Korea.
authors.forename: Heeseob
authors.initials: H
authors.lastname: Lee
mesh_terms: D000704:Analysis of Variance; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D027761:Asparagus Plant / Q000737:chemistry / Q000201:enzymology* / Q000382:microbiology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004144:Diosgenin / Q000031:analogs & derivatives / Q000494:pharmacology; D005285:Fermentation; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012503:Saponins / Q000494:pharmacology; D058836:Weissella / Q000254:growth & development / Q000378:metabolism*
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D012503:Saponins; C459619:protodioscin; D006821:Hyaluronoglucosaminidase; D004144:Diosgenin
keywords: Asparagus cochinchinensis Merrill; HAase (HAase) inhibition activity; fermentation; response surface methodology (RSM)