title: Characterization of tomato apical stunt viroid isolated from a 24-year old seed lot of Capsicum annuum.
abstract: Tomato apical stunt viroid (TASVd) has been identified in a 24-year old seed lot of Capsicum annuum produced in Taiwan. It is the first finding of TASVd in this plant species. The isolate could be discriminated from all reported isolates of TASVd based on its nucleotide sequence, which showed only 94.8% identity with the most related genotype of TASVd. This discrimination was substantiated by phylogenetic analysis. Inoculation of a RNA extract of contaminated seeds to healthy pepper plants showed that the infectivity of the viroid had remained over time. Nevertheless, no transmission to seedlings was observed.
authors.affiliation: National Plant Protection Organization of the Netherlands, P.O. Box 9102, 6700 HC, Wageningen, The Netherlands. j.t.j.verhoeven@nvwa.nl.
authors.forename: J Th J
authors.identifier: http://orcid.org/0000-0002-7362-3079
authors.initials: JTJ
authors.lastname: Verhoeven
authors.affiliation: Naktuinbouw, P.O. Box 40, 2370 AA, Roelofarendsveen, The Netherlands.
authors.forename: H M S
authors.initials: HMS
authors.lastname: Koenraadt
authors.affiliation: National Plant Protection Organization of the Netherlands, P.O. Box 9102, 6700 HC, Wageningen, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: Westenberg
authors.affiliation: National Plant Protection Organization of the Netherlands, P.O. Box 9102, 6700 HC, Wageningen, The Netherlands.
authors.forename: J W
authors.initials: JW
authors.lastname: Roenhorst
mesh_terms: D001483:Base Sequence; D002212:Capsicum / Q000254:growth & development* / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D012639:Seeds / Q000821:virology*; D013624:Taiwan; D014772:Viroids / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D012367:RNA, Viral