title: Impact of blue, red, and far-red light treatments on gene expression and steviol glycoside accumulation in Stevia rebaudiana.
abstract: Stevia rebaudiana (Bertoni) Bertoni is a plant that biosynthesizes a group of natural sweeteners that are up to approximately 400 times sweeter than sucrose. The sweetening components of S. rebaudiana are steviol glycosides (SGs) that partially share their biosynthesis pathway with gibberellins (GAs). However, the molecular mechanisms through which SGs levels can be improved have not been studied. Therefore, transcription levels of several SG biosynthesis-related genes were analyzed under several light treatments involved in GA biosynthesis. We detected higher transcription of UGT85C2, which is one of the UDP-glycosyltransferases (UGTs) involved in catalyzing the sugar-transfer reaction, under red/far-red (R/FR) 1.22 light-emitting diodes (LEDs) and blue LEDs treatment. In this study, it was demonstrated that transcription levels of SG-related genes and the SGs content are affected by light treatments known to affect the GA contents. It is expected that this approach could serve as a practical way to increase SG contents using specific light treatments.
authors.affiliation: Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Yoneda
authors.affiliation: Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakashima
authors.affiliation: Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan.
authors.forename: Juro
authors.initials: J
authors.lastname: Miyasaka
authors.affiliation: Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan.
authors.forename: Katsuaki
authors.initials: K
authors.lastname: Ohdoi
authors.affiliation: Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan. Electronic address: hshimizu@kais.kyoto-u.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimizu
mesh_terms: D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D045786:Diterpenes, Kaurane / Q000096:biosynthesis*; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects*; D005875:Gibberellins / Q000096:biosynthesis; D006027:Glycosides / Q000096:biosynthesis*; D016695:Glycosyltransferases / Q000378:metabolism; D008027:Light; D010940:Plant Proteins / Q000378:metabolism; D036625:Stevia / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects
chemical_list: D045786:Diterpenes, Kaurane; D005875:Gibberellins; D006027:Glycosides; D010940:Plant Proteins; C012042:steviol; D003577:Cytochrome P-450 Enzyme System; C066398:ent-kaurene oxidase; D016695:Glycosyltransferases
keywords: Asteraceae; Cryptochrome; Gibberellin; Light treatments; Phytochrome; Shade-avoidance responses; Stevia rebaudiana; Steviol glycosides; UDP-glycosyltransferases