title: Hyper-Elongation in Colorectal Cancer Tissue - Cerotic Acid is a Potential Novel Serum Metabolic Marker of Colorectal Malignancies.
abstract: OBJECTIVE
  Colorectal cancer (CRC) cells show some alterations of lipid metabolism. Elongation of fatty acids (FA) has not been studied in CRC tissues thus far. The aim of this study was to verify if CRC specimens and normal colon mucosa differ in terms of their levels of very long-chain FAs, a product of FA elongation. Moreover, the expression of elongase genes has been studied in normal tissue and CRC. Finally, we searched for some specific products of FA elongation in serum of CRC patients.
  
  
  METHODS
  The specimens of normal colon mucosa and CRC were obtained from nineteen CRC patients differ in terms of FA elongation. We also searched for some specific products of FA elongation in serum of CRC patients and from healthy volunteers. Tissue and serum FA profiles were determined by means of gas chromatography-mass spectrometry (GC/MS), and the tissue expression of elongases (ELOVLs) was analyzed with real-time PCR.
  
  
  RESULTS
  Compared to normal colon tissue, CRC specimens showed significantly higher levels of 22-, 24- and 26-carbon FAs, stronger expressions of ELOVL1 and ELOVL6 (4- and 9-fold elevated respectively), and higher values of 18: 0/16: 0 elongation index. We also demonstrated presence of cerotic acid (26: 0) in serum of all CRC patients but in none of the healthy controls.
  
  
  CONCLUSIONS
  CRC tissue seems to be characterized by enhanced FA elongation (hyper-elongation). Presence of cerotic acid in CRC patients sera and absence of this FA in healthy subjects points to this compound as a strong candidate for specific metabolic marker of colorectal malignancies.
authors.forename: Adriana
authors.initials: A
authors.lastname: Mika
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Kobiela
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Czumaj
authors.forename: Michał
authors.initials: M
authors.lastname: Chmielewski
authors.forename: Piotr
authors.initials: P
authors.lastname: Stepnowski
authors.forename: Tomasz
authors.initials: T
authors.lastname: Sledzinski
mesh_terms: D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D016022:Case-Control Studies; D003106:Colon / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D000081031:Fatty Acid Elongases; D005227:Fatty Acids / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging
chemical_list: D014408:Biomarkers, Tumor; C000630949:ELOVL1 protein, human; C000630955:ELOVL6 protein, human; D005227:Fatty Acids; C017364:hexacosanoic acid; D000123:Acetyltransferases; D000081031:Fatty Acid Elongases
keywords: Cerotic acid; Colon mucosa; Colorectal cancer; Elongase; Fatty acids; Gas chromatography-mass spectrometry; Serum