title: PCR fishing for red endophytes in British Columbia Kallymeniaceae (Gigartinales, Florideophyceae) uncovers the species-rich Kallymenicola gen. nov. (Palmariales, Nemaliophycidae).
abstract: Unexpected contaminants uncovered during routine COI-5P DNA barcoding of British Columbia Kallymeniaceae indicated the presence of a novel lineage allied to the family Meiodiscaceae, Palmariales. Available rbcL data for species of this family were used to design specific primers to screen for the presence of the meiodiscacean species in 534 kallymeniacean specimens primarily from British Columbia, Canada. Ultimately, 43 positive PCR products representing six diverse genetic groups from nine host species were uncovered; three are described here in the new genus Kallymenicola gen. nov., viz., K. invisiblis sp. nov., K penetrans sp. nov., and K superficialis sp. nov. Although genetic groups loosely displayed evidence of host specificity and cospeciation, examples of host switching with interesting biogeographical patterns were also documented.
authors.affiliation: Centre for Environmental & Molecular Algal Research, Department of Biology, University of New Brunswick, Fredericton, New Brunswick, Canada, E3B 5A3.
authors.forename: Joshua R
authors.initials: JR
authors.lastname: Evans
authors.affiliation: Centre for Environmental & Molecular Algal Research, Department of Biology, University of New Brunswick, Fredericton, New Brunswick, Canada, E3B 5A3.
authors.forename: Gary W
authors.initials: GW
authors.lastname: Saunders
mesh_terms: D001955:British Columbia; D058893:DNA Barcoding, Taxonomic; D060026:Endophytes / Q000145:classification* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D000461:Rhodophyta / Q000145:classification* / Q000235:genetics*; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*
chemical_list: C101625:RbcL protein, plastid; D012273:Ribulose-Bisphosphate Carboxylase
keywords: 
  Kallymenicola
  ; COI-5P; Kallymeniaceae; Meiodiscaceae; Palmariales; rbcL