title: Isolation, biochemical characterization and antibiofilm effect of a lectin from the marine sponge Aplysina lactuca.
abstract: A new lectin was isolated from the marine sponge Aplysina lactuca (ALL) by combining ammonium sulfate precipitation and affinity chromatography on guar gum matrix. ALL showed affinity for the disaccharides α-lactose, β-lactose and lactulose (Ka=12.5, 31.9 and 145.5M-1, respectively), as well as the glycoprotein porcine stomach mucin. Its hemagglutinating activity was stable in neutral acid pH values and temperatures below 60°C. ALL is a dimeric protein formed by two covalently linked polypeptide chains. The average molecular mass, as determined by Electrospray Ionization Mass Spectrometry (ESI-MS), was 31,810±2Da. ESI-MS data also indicated the presence of three cysteines involved in one intrachain and one interchain disulfide bond. The partial amino acid sequence of ALL was determined by tandem mass spectrometry. Eight tryptic peptides presented similarity with lectin I isolated from Axinella polypoides. Its secondary structure is predominantly β-sheet, as indicated by circular dichroism (CD) spectroscopy. ALL agglutinated gram-positive and gram-negative bacterial cells, and it were able to significantly reduce the biomass of the bacterial biofilm tested at dose- dependent effect.
authors.affiliation: Laboratório de Biotecnologia Marinha - BioMar-Lab, Departamento de Engenharia de Pesca, Universidade Federal do Ceará, Campus do Pici s/n, Bloco 871, 60440-970, Fortaleza, Ceará, Brazil.
authors.forename: Rômulo Farias
authors.initials: RF
authors.lastname: Carneiro
authors.affiliation: Laboratório de Biotecnologia Marinha - BioMar-Lab, Departamento de Engenharia de Pesca, Universidade Federal do Ceará, Campus do Pici s/n, Bloco 871, 60440-970, Fortaleza, Ceará, Brazil.
authors.forename: Paulo Henrique Pinheiro de
authors.initials: PHP
authors.lastname: Lima
authors.affiliation: Laboratório de Biotecnologia Marinha - BioMar-Lab, Departamento de Engenharia de Pesca, Universidade Federal do Ceará, Campus do Pici s/n, Bloco 871, 60440-970, Fortaleza, Ceará, Brazil.
authors.forename: Renata Pinheiro
authors.initials: RP
authors.lastname: Chaves
authors.affiliation: Laboratório Integrado de Biomoléculas - LIBS, Departamento de Patologia e Medicina Legal, Universidade Federal do Ceará, Monsenhor Furtado, s/n, 60430-160, Fortaleza, Ceará, Brazil.
authors.forename: Rafael
authors.initials: R
authors.lastname: Pereira
authors.affiliation: Laboratório Integrado de Biomoléculas - LIBS, Departamento de Patologia e Medicina Legal, Universidade Federal do Ceará, Monsenhor Furtado, s/n, 60430-160, Fortaleza, Ceará, Brazil.
authors.forename: Anna Luísa
authors.initials: AL
authors.lastname: Pereira
authors.affiliation: Laboratório Integrado de Biomoléculas - LIBS, Departamento de Patologia e Medicina Legal, Universidade Federal do Ceará, Monsenhor Furtado, s/n, 60430-160, Fortaleza, Ceará, Brazil.
authors.forename: Mayron Alves
authors.initials: MA
authors.lastname: de Vasconcelos
authors.affiliation: Departamento de Zoologia, UFPE - Universidade Federal de Pernambuco, Av. Prof Moraes Rego, 1235, 50670-901, Cidade Universitária, Recife, Pernambuco, Brazil.
authors.forename: Ulisses
authors.initials: U
authors.lastname: Pinheiro
authors.affiliation: Laboratório Integrado de Biomoléculas - LIBS, Departamento de Patologia e Medicina Legal, Universidade Federal do Ceará, Monsenhor Furtado, s/n, 60430-160, Fortaleza, Ceará, Brazil.
authors.forename: Edson Holanda
authors.initials: EH
authors.lastname: Teixeira
authors.affiliation: Laboratório de Biotecnologia Marinha - BioMar-Lab, Departamento de Engenharia de Pesca, Universidade Federal do Ceará, Campus do Pici s/n, Bloco 871, 60440-970, Fortaleza, Ceará, Brazil.
authors.forename: Celso Shiniti
authors.initials: CS
authors.lastname: Nagano
authors.affiliation: Laboratório de Biotecnologia Marinha - BioMar-Lab, Departamento de Engenharia de Pesca, Universidade Federal do Ceará, Campus do Pici s/n, Bloco 871, 60440-970, Fortaleza, Ceará, Brazil. Electronic address: alexholandasampaio@gmail.com.
authors.forename: Alexandre Holanda
authors.initials: AH
authors.lastname: Sampaio
mesh_terms: D000645:Ammonium Sulfate / Q000737:chemistry; D000818:Animals; D018441:Biofilms / Q000187:drug effects*; D002241:Carbohydrates / Q000032:analysis; D011232:Chemical Precipitation; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology; D006384:Hemagglutination / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D008970:Molecular Weight; D011161:Porifera / Q000737:chemistry*; D011817:Rabbits; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology
chemical_list: D002241:Carbohydrates; D037102:Lectins; D000645:Ammonium Sulfate
keywords: Circular dichroism; Lectin; Mass spectrometry; Protein characterization; Purification; Sponge