title: Potential Usefulness ofStreptococcus pneumoniaeExtracellular Membrane Vesicles as Antibacterial Vaccines.
abstract: The secretion of extracellular membrane vesicles (EMVs) is a common phenomenon that occurs in archaea, bacteria, and mammalian cells. The EMVs of bacteria play important roles in their virulence, biogenesis mechanisms, and host cell interactions. Bacterial EMVs have recently become the focus of attention because of their potential as highly effective vaccines that cause few side effects. Here, we isolated the EMVs ofStreptococcus pneumoniaeand examined their potential as new vaccine candidates. Although theS. pneumoniaebacteria were highly pathogenic in a mouse model, the EMVs purified from these bacteria showed low pathological activity both in cell culture and in mice. When mice were injected intraperitoneally withS. pneumoniaeEMVs and then challenged, they were protected from both the homologous strain and another pathogenic serotype ofS. pneumoniae. We also identified a number of proteins that may have immunogenic activity and may be responsible for the immune responses by the hosts. These results suggest thatS. pneumoniaeEMVs or their individual immunogenic antigens may be useful as new vaccine agents.
authors.affiliation: Division of Bioconvergence Analysis, Korea Basic Science Institute, Daejeon, Republic of Korea; Tunneling Nanotube Research Center, Division of Life Science, Korea University, Seoul 02841, Republic of Korea.
authors.forename: Chi-Won
authors.initials: CW
authors.lastname: Choi
authors.affiliation: Division of Bioconvergence Analysis, Korea Basic Science Institute, Daejeon, Republic of Korea; Department of Bio-Analytical Science, University of Science and Technology, Daejeon, Republic of Korea; Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea.
authors.forename: Edmond Changkyun
authors.initials: EC
authors.lastname: Park
authors.affiliation: Division of Bioconvergence Analysis, Korea Basic Science Institute, Daejeon, Republic of Korea.
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Yun
authors.affiliation: Division of Bioconvergence Analysis, Korea Basic Science Institute, Daejeon, Republic of Korea; Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea.
authors.forename: Sang-Yeop
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Division of Bioconvergence Analysis, Korea Basic Science Institute, Daejeon, Republic of Korea; Department of Bio-Analytical Science, University of Science and Technology, Daejeon, Republic of Korea; Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea.
authors.forename: Seung Il
authors.identifier: 0000-0001-6681-4486
authors.initials: SI
authors.lastname: Kim
authors.affiliation: Division of Bioconvergence Analysis, Korea Basic Science Institute, Daejeon, Republic of Korea; Tunneling Nanotube Research Center, Division of Life Science, Korea University, Seoul 02841, Republic of Korea; Department of Functional Genomics, University of Science and Technology, Daejeon, Republic of Korea.
authors.forename: Gun-Hwa
authors.identifier: 0000-0003-0360-4435
authors.initials: GH
authors.lastname: Kim
mesh_terms: D000072283:A549 Cells; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000276:immunology*; D055808:Drug Discovery; D000067128:Extracellular Vesicles / Q000737:chemistry / Q000276:immunology* / Q000648:ultrastructure; D006801:Humans; D007274:Injections, Intraperitoneal; D051379:Mice; D011008:Pneumococcal Infections / Q000382:microbiology / Q000517:prevention & control*; D022242:Pneumococcal Vaccines / Q000008:administration & dosage / Q000009:adverse effects / Q000737:chemistry*; D013296:Streptococcus pneumoniae / Q000166:cytology*; D014611:Vaccination; D014774:Virulence
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D022242:Pneumococcal Vaccines