title: Circulating miR-155, miR-145 and let-7c as diagnostic biomarkers of the coronary artery disease.
abstract: Coronary artery disease (CAD) is the most prevalent cause of mortality and morbidity worldwide and the number of individuals at risk is increasing. To better manage cardiovascular diseases, improved tools for risk prediction including the identification of novel accurate biomarkers are needed. MicroRNA (miRNA) are essential post-transcriptional modulators of gene expression leading to mRNA suppression or translational repression. Specific expression profiles of circulating miRNA have emerged as potential noninvasive diagnostic biomarkers of diseases. The aim of this study was to identify the potential diagnostic value of circulating miRNA with CAD. Circulating miR-145, miR-155, miR-92a and let-7c were selected and validated by quantitative PCR in 69 patients with CAD and 30 control subjects from the cross-sectional study GENES. The expression of miR-145, miR-155 and let-7c showed significantly reduced expression in patients with CAD compared to controls. Multivariate logistic regression analysis revealed that low levels of circulating let-7c, miR-145 and miR-155 were associated with CAD. Receiver operating curves analysis showed that let-7c, miR-145 or miR-155 were powerful markers for detecting CAD. Furthermore, we demonstrated that the combination of the three circulating miRNA managed to deliver a specific signature for diagnosing CAD.
authors.affiliation: INSERM UMR-1048, Institute of Metabolic and Cardiovascular Diseases, Toulouse, France.|Toulouse Paul Sabatier University, Toulouse, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Faccini
authors.affiliation: Toulouse Paul Sabatier University, Toulouse, France.|CHU Toulouse, Department of Cardiology, Toulouse, France.|INSERM UMR-1027, Epidémiologie et Analyses en Santé publique, Toulouse, France.
authors.forename: Jean-Bernard
authors.initials: JB
authors.lastname: Ruidavets
authors.affiliation: INSERM UMR-1037, Cancer Research Center of Toulouse, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Cordelier
authors.affiliation: PlateformeGeT, Toulouse, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Martins
authors.affiliation: PlateformeGeT, Toulouse, France.
authors.forename: Jean-José
authors.initials: JJ
authors.lastname: Maoret
authors.affiliation: Toulouse Paul Sabatier University, Toulouse, France.|CHU Toulouse, Department of Cardiology, Toulouse, France.
authors.forename: Vanina
authors.initials: V
authors.lastname: Bongard
authors.affiliation: Toulouse Paul Sabatier University, Toulouse, France.|CHU Toulouse, Department of Cardiology, Toulouse, France.|INSERM UMR-1027, Epidémiologie et Analyses en Santé publique, Toulouse, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Ferrières
authors.affiliation: CHU Toulouse, Department of Cardiology, Toulouse, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Roncalli
authors.affiliation: INSERM UMR-1048, Institute of Metabolic and Cardiovascular Diseases, Toulouse, France.|Toulouse Paul Sabatier University, Toulouse, France.|CHU Toulouse, Department of Cardiology, Toulouse, France.
authors.forename: Meyer
authors.initials: M
authors.lastname: Elbaz
authors.affiliation: INSERM UMR-1048, Institute of Metabolic and Cardiovascular Diseases, Toulouse, France.|Toulouse Paul Sabatier University, Toulouse, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Vindis
mesh_terms: D000368:Aged; D019540:Area Under Curve; D015415:Biomarkers / Q000097:blood*; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000175:diagnosis* / Q000235:genetics; D003430:Cross-Sectional Studies; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D015415:Biomarkers; C541010:MIRN145 microRNA, human; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; C487931:mirnlet7 microRNA, human