title: Flow Dynamics and HSPC Homing in Bone Marrow Microvessels.
abstract: Measurements of flow velocities at the level of individual arterial vessels and sinusoidal capillaries are crucial for understanding the dynamics of hematopoietic stem and progenitor cell homing in the bone marrow vasculature. We have developed two complementary intravital two-photon imaging approaches to determine blood flow dynamics and velocities in multiple vessel segments by capturing the motion of red blood cells. High-resolution spatiotemporal measurements through a cranial window to determine short-time dynamics of flowing blood cells and repetitive centerline scans were used to obtain a detailed flow-profile map with hemodynamic parameters. In addition, we observed the homing of individual hematopoietic stem and progenitor cells and obtained detailed information on their homing behavior. With our imaging setup, we determined flow patterns at cellular resolution, blood flow velocities and wall shear stress in small arterial vessels and highly branched sinusoidal capillaries, and the cellular dynamics of hematopoietic stem and progenitor cell homing.
authors.affiliation: Department of Tissue Morphogenesis, Max Planck Institute for Molecular Biomedicine, 48149 Münster, Germany; Faculty of Medicine, University of Münster, 48149 Münster, Germany. Electronic address: mgbixel@mpi-muenster.mpg.de.
authors.forename: M Gabriele
authors.initials: MG
authors.lastname: Bixel
authors.affiliation: Department of Tissue Morphogenesis, Max Planck Institute for Molecular Biomedicine, 48149 Münster, Germany.
authors.forename: Anjali P
authors.initials: AP
authors.lastname: Kusumbe
authors.affiliation: Department of Tissue Morphogenesis, Max Planck Institute for Molecular Biomedicine, 48149 Münster, Germany.
authors.forename: Saravana K
authors.initials: SK
authors.lastname: Ramasamy
authors.affiliation: Department of Tissue Morphogenesis, Max Planck Institute for Molecular Biomedicine, 48149 Münster, Germany.
authors.forename: Kishor K
authors.initials: KK
authors.lastname: Sivaraj
authors.affiliation: Department of Vascular Cell Biology, Max Planck Institute for Molecular Biomedicine, 48149 Münster, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Butz
authors.affiliation: Department of Vascular Cell Biology, Max Planck Institute for Molecular Biomedicine, 48149 Münster, Germany.
authors.forename: Dietmar
authors.initials: D
authors.lastname: Vestweber
authors.affiliation: Department of Tissue Morphogenesis, Max Planck Institute for Molecular Biomedicine, 48149 Münster, Germany; Faculty of Medicine, University of Münster, 48149 Münster, Germany. Electronic address: ralf.adams@mpi-muenster.mpg.de.
authors.forename: Ralf H
authors.initials: RH
authors.lastname: Adams
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology*; D001853:Bone Marrow / Q000502:physiology*; D001854:Bone Marrow Cells / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006439:Hemodynamics / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D055806:Microvessels / Q000502:physiology*; D033081:Shear Strength / Q000502:physiology; D013312:Stress, Physiological / Q000502:physiology
keywords: blood flow velocities; bone marrow; deep tissue imaging; hematopoietic parameters; hematopoietic stem cell; intravital imaging; microvasculature; stem cell homing; wall shear stress