title: Expression of MicroRNA in Host Cells Infected withHelicobacter pylori.
abstract: OBJECTIVE
  MicroRNAs (miRNAs) regulate gene expression. We assess miRNA regulation byHelicobacter pyloriinfection and elucidate their role inH. pylori-infected gastric epithelial cells.
  
  
  METHODS
  The relationship between miRNA expression and DNA methylation was examined. Cells were treated with the nuclear factor-kappaB (NF-κB) inhibitor Bay 11-7082 to determine the relationship between miRNA expression and NF-κB signal transduction.
  
  
  RESULTS
  In the negative control cells infected withH. pylori26695, the expression of six miRNAs was increased, whereas the expression of five miRNAs was decreased. The expression of upregulated miRNAs was increased when the host cells were treated withH. pyloriand an NF-κB inhibitor. miR-127-5p, -155, and -181 were associated with increased interleukin 6 (IL-6) secretion inH. pyloriinfected cells treated with anti-miRNA. The expression of miR-155, -127-5p, -195, -216, -206, and -488 increased by approximately 3-fold following treatment with the methylation inhibitor Aza.
  
  
  CONCLUSIONS
  We found novel miRNAs inH. pylori-infected negative control cells using miRNA microarrays. Upregulated miRNA expression was inversely related to the transcription of NF-κB. miR-195 and miR-488 appear to play a pivotal role in controlling IL-6 activity inH. pyloriinfection. miRNA expression inH. pyloriinfection was affected by methylation.
authors.affiliation: Department of Internal Medicine, Gachon University Gil Medical Center, Incheon, Korea.
authors.forename: Jun-Won
authors.initials: JW
authors.lastname: Chung
authors.affiliation: Department of Internal Medicine, Catholic Kwandong University International St. Mary's Hospital, Incheon, Korea.
authors.forename: Seok Hoo
authors.initials: SH
authors.lastname: Jeong
authors.affiliation: Department of Convergence Medicine and Asan Institute for Life Sciences, Seoul, Korea.
authors.forename: Sun Mi
authors.initials: SM
authors.lastname: Lee
authors.affiliation: Department of Convergence Medicine and Asan Institute for Life Sciences, Seoul, Korea.
authors.forename: Jhang Ho
authors.initials: JH
authors.lastname: Pak
authors.affiliation: Department of Gastroenterology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Gin Hyug
authors.initials: GH
authors.lastname: Lee
authors.affiliation: Department of Convergence Medicine and Asan Institute for Life Sciences, Seoul, Korea.
authors.forename: Jin-Yong
authors.initials: JY
authors.lastname: Jeong
authors.affiliation: Department of Gastroenterology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Kim
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D019175:DNA Methylation / Q000187:drug effects; D004847:Epithelial Cells / Q000382:microbiology; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology; D016481:Helicobacter Infections / Q000188:drug therapy / Q000235:genetics*; D016480:Helicobacter pylori / Q000235:genetics*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D009570:Nitriles / Q000494:pharmacology*; D015398:Signal Transduction / Q000235:genetics; D013450:Sulfones / Q000494:pharmacology*; D015854:Up-Regulation / Q000502:physiology; D055815:Young Adult
chemical_list: C434003:3-(4-methylphenylsulfonyl)-2-propenenitrile; C508600:IL6 protein, human; D015850:Interleukin-6; C552722:MIRN195 microRNA, human; C561403:MIRN488 microRNA, human; D035683:MicroRNAs; D016328:NF-kappa B; D009570:Nitriles; D013450:Sulfones
keywords: Cytokines; Helicobacter pylori; MicroRNAs; NF-kappa B; Negative control cell lines