title: Separation and Characterization of Angiotensin I Converting Enzyme (ACE) Inhibitory Peptides from Saurida elongata Proteins Hydrolysate by IMAC-Ni2.
abstract: Lizard fish protein hydrolysates (LFPH) were prepared from Lizard fish (Saurida elongata) proteins possessing powerful angiotensin I converting enzyme (ACE) inhibitory activity and the fraction (LFPH-I) with high ACE inhibitory activity was obtained through ultrafiltration. The active Fraction (F2) was isolated from LFPH-I using immobilized metal affinity chromatography (IMAC-Ni2+). Analysis of amino acid levels revealed that F2 eluted from IMAC was enriched in Met, His, Tyr, Pro, Ile, and Leu compared to the crude peptide LFPH-I. F2 with the high ACE inhibitory activity (IC50of 0.116 mg·mL-1) was further separated by a reverse-phase column to yield a novel ACE inhibitory peptide with IC50value of 52 μM. The ACE inhibitory peptide was identified as Arg-Tyr-Arg-Pro, RYRP. The present study demonstrated that IMAC may be a useful tool for the separation of ACE inhibitory peptides from protein hydrolysate.
authors.affiliation: Guangxi Colleges and Universities Key Laboratory of New Technology and Application in Resource Chemical Engineering, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China. binglin0628@163.com.
authors.forename: Lixia
authors.initials: L
authors.lastname: Sun
authors.affiliation: Medical College, Guangxi University of Science and Technology, Liuzhou 545006, China. wusg1974@163.com.
authors.forename: Shanguang
authors.initials: S
authors.lastname: Wu
authors.affiliation: Guangxi Colleges and Universities Key Laboratory of New Technology and Application in Resource Chemical Engineering, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China. zhouliqin100430@163.com.
authors.forename: Liqin
authors.initials: L
authors.lastname: Zhou
authors.affiliation: College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China. wangf@mail.buct.edu.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: Guangxi Colleges and Universities Key Laboratory of New Technology and Application in Resource Chemical Engineering, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China. lanxiongdiao@163.com.
authors.forename: Xiongdiao
authors.initials: X
authors.lastname: Lan
authors.affiliation: Guangxi Colleges and Universities Key Laboratory of New Technology and Application in Resource Chemical Engineering, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China. sunjhgx@163.com.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Sun
authors.affiliation: Guangxi Colleges and Universities Key Laboratory of New Technology and Application in Resource Chemical Engineering, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China. zhftong@sina.com.
authors.forename: Zhangfa
authors.initials: Z
authors.lastname: Tong
authors.affiliation: Guangxi Colleges and Universities Key Laboratory of New Technology and Application in Resource Chemical Engineering, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China. liaodankuigx@163.com.
authors.forename: Dankui
authors.initials: D
authors.lastname: Liao
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D000818:Animals; D005591:Chemical Fractionation; D002846:Chromatography, Affinity / Q000379:methods*; D056148:Chromatography, Reverse-Phase; D029941:Fish Proteins / Q000737:chemistry / Q000302:isolation & purification*; D005399:Fishes; D009842:Oligopeptides / Q000302:isolation & purification*; D011492:Protein Hydrolysates / Q000737:chemistry*; D014462:Ultrafiltration
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D029941:Fish Proteins; D009842:Oligopeptides; D011492:Protein Hydrolysates
keywords: ACE-inhibitory activity; IMAC; hydrolysate; lizard fish; purification