title: Th17 cells and neutrophils: Close collaborators in chronic Leishmania mexicana infections leading to disease severity.
abstract: Cutaneous leishmaniasis caused by Leishmania mexicana is associated with an important inflammatory response. We here analysed the kinetics of Th17 cells and neutrophils in ear lobe lesions caused by Leishmania mexicana throughout 90 days of disease progression in susceptible BALB/c and semi-resistant C57BL/6 mice infected with 1 × 105Leishmania mexicana promastigotes. Cells in the lesions were extracted and quantified by flow cytometry, whereas their distribution in the tissues in relation to the parasites was analysed by immunohistochemistry. Our results show that in BALB/c mice, both Th17 cells and neutrophils increase concomitantly and to significantly higher levels on day 90 post-infection, as compared to C57BL/6 mice. Our results provide novel evidence on the cells causing chronic inflammation throughout Leishmania mexicana infections, resulting as a consequence of neutrophil recruitment together with Th17 cell differentiation and recruitment, both of which remain in the infection site throughout the late phase of the infection. We conclude that the more enhanced levels of Th17 cells and neutrophils during chronic inflammatory lesions in BALB/c mice participate in their enhanced susceptibility towards a progressive disease evolution, whereas the more controlled response of these cells in C57BL/6 mice possibly relates to the more resistant profile of this mouse strain.
authors.affiliation: Facultad de Medicina, Unidad de Investigación en Medicina Experimental, Universidad Nacional Autónoma de México (UNAM), Hospital General de México, Ciudad de México, México.
authors.forename: C P
authors.initials: CP
authors.lastname: Pedraza-Zamora
authors.affiliation: Facultad de Medicina, Unidad de Investigación en Medicina Experimental, Universidad Nacional Autónoma de México (UNAM), Hospital General de México, Ciudad de México, México.
authors.forename: J
authors.initials: J
authors.lastname: Delgado-Domínguez
authors.affiliation: Facultad de Medicina, Unidad de Investigación en Medicina Experimental, Universidad Nacional Autónoma de México (UNAM), Hospital General de México, Ciudad de México, México.
authors.forename: J
authors.initials: J
authors.lastname: Zamora-Chimal
authors.affiliation: Facultad de Medicina, Unidad de Investigación en Medicina Experimental, Universidad Nacional Autónoma de México (UNAM), Hospital General de México, Ciudad de México, México.
authors.forename: I
authors.identifier: 0000-0003-3821-4998
authors.initials: I
authors.lastname: Becker
mesh_terms: D000818:Animals; D002908:Chronic Disease; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D005260:Female; D007894:Leishmania mexicana / Q000502:physiology*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology* / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D020556:Neutrophil Infiltration; D009504:Neutrophils / Q000276:immunology*; D013045:Species Specificity; D058504:Th17 Cells / Q000276:immunology*
keywords: 
  Leishmania mexicana
  ; IL-17; Th17 cells; inflammation; neutrophils