title: Characterization and therapeutic application of canine adipose mesenchymal stem cells to treat elbow osteoarthritis.
abstract: Visceral adipose tissue (AT) obtained from surgical waste during routine ovariectomies was used as a source for isolating canine mesenchymal stem cells (MSCs). As determined by cytofluorimetry, passage 2 cells expressed MSC markers CD44 and CD90 and were negative for lineage-specific markers CD34 and CD45. The cells differentiated toward osteogenic, adipogenic, and chondrogenic directions. With therapeutic aims, 30 dogs (39 joints) suffering from elbow dysplasia (ED) and osteoarthritis (OA) were intra-articularly transplanted with allogeneic MSCs suspended in 0.5% hyaluronic acid (HA). A highly significant improvement was achieved without any medication as demonstrated by the degree of lameness during the follow-up period of 1 y. Control arthroscopy of 1 transplanted dog indicated that the cartilage had regenerated. Histological analysis of the cartilage biopsy confirmed that the regenerated cartilage was of hyaline type. These results demonstrate that transplantation of allogeneic adipose tissue-derived mesenchymal stem cells (AT-MSCs) is a novel, noninvasive, and highly effective therapeutic tool in treating canine elbow dysplasia.
authors.affiliation: Visal Plus Ltd. (Kriston-Pál), Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, H-6726 Szeged, Temesvári krt. 62 (Czibula, Haracska, Monostori), Delta Bio 2000 Ltd. (Gyuris), University of Szeged, Faculty of General Medicine, Department of Pathology, Szeged, Hungary (Sükösd); University of Veterinary Science (Süth), Department of Pathology (Balka), FeliCaVet Animal Hospital (Seregi), National Blood Service, Budapest, Hungary (Uher); University of Sheffield, Sheffield, South Yorkshire, United Kingdom (Kiss-Tóth).
authors.forename: Éva
authors.initials: É
authors.lastname: Kriston-Pál
authors.affiliation: Visal Plus Ltd. (Kriston-Pál), Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, H-6726 Szeged, Temesvári krt. 62 (Czibula, Haracska, Monostori), Delta Bio 2000 Ltd. (Gyuris), University of Szeged, Faculty of General Medicine, Department of Pathology, Szeged, Hungary (Sükösd); University of Veterinary Science (Süth), Department of Pathology (Balka), FeliCaVet Animal Hospital (Seregi), National Blood Service, Budapest, Hungary (Uher); University of Sheffield, Sheffield, South Yorkshire, United Kingdom (Kiss-Tóth).
authors.forename: Ágnes
authors.initials: Á
authors.lastname: Czibula
authors.affiliation: Visal Plus Ltd. (Kriston-Pál), Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, H-6726 Szeged, Temesvári krt. 62 (Czibula, Haracska, Monostori), Delta Bio 2000 Ltd. (Gyuris), University of Szeged, Faculty of General Medicine, Department of Pathology, Szeged, Hungary (Sükösd); University of Veterinary Science (Süth), Department of Pathology (Balka), FeliCaVet Animal Hospital (Seregi), National Blood Service, Budapest, Hungary (Uher); University of Sheffield, Sheffield, South Yorkshire, United Kingdom (Kiss-Tóth).
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Gyuris
authors.affiliation: Visal Plus Ltd. (Kriston-Pál), Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, H-6726 Szeged, Temesvári krt. 62 (Czibula, Haracska, Monostori), Delta Bio 2000 Ltd. (Gyuris), University of Szeged, Faculty of General Medicine, Department of Pathology, Szeged, Hungary (Sükösd); University of Veterinary Science (Süth), Department of Pathology (Balka), FeliCaVet Animal Hospital (Seregi), National Blood Service, Budapest, Hungary (Uher); University of Sheffield, Sheffield, South Yorkshire, United Kingdom (Kiss-Tóth).
authors.forename: Gyula
authors.initials: G
authors.lastname: Balka
authors.affiliation: Visal Plus Ltd. (Kriston-Pál), Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, H-6726 Szeged, Temesvári krt. 62 (Czibula, Haracska, Monostori), Delta Bio 2000 Ltd. (Gyuris), University of Szeged, Faculty of General Medicine, Department of Pathology, Szeged, Hungary (Sükösd); University of Veterinary Science (Süth), Department of Pathology (Balka), FeliCaVet Animal Hospital (Seregi), National Blood Service, Budapest, Hungary (Uher); University of Sheffield, Sheffield, South Yorkshire, United Kingdom (Kiss-Tóth).
authors.forename: Antal
authors.initials: A
authors.lastname: Seregi
authors.affiliation: Visal Plus Ltd. (Kriston-Pál), Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, H-6726 Szeged, Temesvári krt. 62 (Czibula, Haracska, Monostori), Delta Bio 2000 Ltd. (Gyuris), University of Szeged, Faculty of General Medicine, Department of Pathology, Szeged, Hungary (Sükösd); University of Veterinary Science (Süth), Department of Pathology (Balka), FeliCaVet Animal Hospital (Seregi), National Blood Service, Budapest, Hungary (Uher); University of Sheffield, Sheffield, South Yorkshire, United Kingdom (Kiss-Tóth).
authors.forename: Farkas
authors.initials: F
authors.lastname: Sükösd
authors.affiliation: Visal Plus Ltd. (Kriston-Pál), Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, H-6726 Szeged, Temesvári krt. 62 (Czibula, Haracska, Monostori), Delta Bio 2000 Ltd. (Gyuris), University of Szeged, Faculty of General Medicine, Department of Pathology, Szeged, Hungary (Sükösd); University of Veterinary Science (Süth), Department of Pathology (Balka), FeliCaVet Animal Hospital (Seregi), National Blood Service, Budapest, Hungary (Uher); University of Sheffield, Sheffield, South Yorkshire, United Kingdom (Kiss-Tóth).
authors.forename: Miklós
authors.initials: M
authors.lastname: Süth
authors.affiliation: Visal Plus Ltd. (Kriston-Pál), Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, H-6726 Szeged, Temesvári krt. 62 (Czibula, Haracska, Monostori), Delta Bio 2000 Ltd. (Gyuris), University of Szeged, Faculty of General Medicine, Department of Pathology, Szeged, Hungary (Sükösd); University of Veterinary Science (Süth), Department of Pathology (Balka), FeliCaVet Animal Hospital (Seregi), National Blood Service, Budapest, Hungary (Uher); University of Sheffield, Sheffield, South Yorkshire, United Kingdom (Kiss-Tóth).
authors.forename: Endre
authors.initials: E
authors.lastname: Kiss-Tóth
authors.affiliation: Visal Plus Ltd. (Kriston-Pál), Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, H-6726 Szeged, Temesvári krt. 62 (Czibula, Haracska, Monostori), Delta Bio 2000 Ltd. (Gyuris), University of Szeged, Faculty of General Medicine, Department of Pathology, Szeged, Hungary (Sükösd); University of Veterinary Science (Süth), Department of Pathology (Balka), FeliCaVet Animal Hospital (Seregi), National Blood Service, Budapest, Hungary (Uher); University of Sheffield, Sheffield, South Yorkshire, United Kingdom (Kiss-Tóth).
authors.forename: Lajos
authors.initials: L
authors.lastname: Haracska
authors.affiliation: Visal Plus Ltd. (Kriston-Pál), Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, H-6726 Szeged, Temesvári krt. 62 (Czibula, Haracska, Monostori), Delta Bio 2000 Ltd. (Gyuris), University of Szeged, Faculty of General Medicine, Department of Pathology, Szeged, Hungary (Sükösd); University of Veterinary Science (Süth), Department of Pathology (Balka), FeliCaVet Animal Hospital (Seregi), National Blood Service, Budapest, Hungary (Uher); University of Sheffield, Sheffield, South Yorkshire, United Kingdom (Kiss-Tóth).
authors.forename: Ferenc
authors.initials: F
authors.lastname: Uher
authors.affiliation: Visal Plus Ltd. (Kriston-Pál), Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, H-6726 Szeged, Temesvári krt. 62 (Czibula, Haracska, Monostori), Delta Bio 2000 Ltd. (Gyuris), University of Szeged, Faculty of General Medicine, Department of Pathology, Szeged, Hungary (Sükösd); University of Veterinary Science (Süth), Department of Pathology (Balka), FeliCaVet Animal Hospital (Seregi), National Blood Service, Budapest, Hungary (Uher); University of Sheffield, Sheffield, South Yorkshire, United Kingdom (Kiss-Tóth).
authors.forename: Éva
authors.initials: É
authors.lastname: Monostori
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000628:therapy*; D004285:Dogs; D005552:Forelimb / Q000601:surgery; D050152:Intra-Abdominal Fat / Q000166:cytology; D007596:Joints / Q000601:surgery; D045164:Mesenchymal Stem Cell Transplantation / Q000662:veterinary*; D010003:Osteoarthritis / Q000628:therapy / Q000662:veterinary*