title: Hairpin-shaped DNA Templated Copper Nanoparticles for Fluorescence Detection of Adenosine Triphosphate Based on Ligation-mediated Exonuclease Cleavage.
abstract: DNA-templated copper nanoparticles (CuNPs) have recently received considerable interest as functional fluorescent probes for biochemical analysis. In this work, a novel ATP-dependent ligation reactions (ATP-DLR) based ATP assay strategy was proposed by using hairpin-shaped (HS) DNA templated CuNPs as a fluorescent probe. Nick sealing by T4 DNA ligase leads to the formation of intact HS DNA, which can resist the exonuclease cleavage and be taken as the template for CuNPs formation, resulting in strong fluorescence. The proposed ATP detection is label free, sensitive and highly selective, and it has good linearity from 0.02 to 4 μM and a detection limit of 7 nM. This strategy is expected to promote the exploitation and application of DNA-templated CuNPs in biochemical and biomedical studies, and holds great promise in fluorescence detection for other ligation-related biomolecules.
authors.affiliation: College of Chemistry and Chemical Engineering, Zhoukou Normal University.
authors.forename: Wenping
authors.initials: W
authors.lastname: Zhu
authors.forename: Liyan
authors.initials: L
authors.lastname: Dai
authors.forename: Zengchen
authors.initials: Z
authors.lastname: Liu
authors.forename: Weijie
authors.initials: W
authors.lastname: Yang
authors.forename: Chunxiang
authors.initials: C
authors.lastname: Zhao
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Li
authors.forename: Yahong
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis* / Q000737:chemistry; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D003300:Copper / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D005092:Exonucleases / Q000378:metabolism*; D055029:Inverted Repeat Sequences; D053768:Metal Nanoparticles / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D015342:DNA Probes; D003300:Copper; D000255:Adenosine Triphosphate; D005092:Exonucleases