title: Biological H2potential harvested from complex gelatinaceous wastewater via attached versus suspended growth culture anaerobes.
abstract: The effect of cultural growth treating gelatinaceous wastewater on hydrogen fermentative was assessed using up-flow multi-stage anaerobic sponge reactor (UMASR) and anaerobic sequencing batch reactor (AnSBR). Both reactors were operated at five hydraulic retention times (HRTs). UMASR achieved the maximum COD removal efficiency of 60.2±4.4% at HRT of 48h. Moreover, UMASR exhibited superiority in the course of carbohydrates and proteins removal efficiencies' of 100 and 52.5±2.4% due to high amylase and protease activities' of 4.1±0.3 and 0.032±0.002U, respectively. Contrariwise, AnSBR assigned for the peak hydrogen production rate of 1.17±0.14L/L/day at HRT of 24-h. Lipase activity was quite high (0.307±0.023U) in AnSBR resulting in removal efficiency of 35.2±2.1% for lipids. Stover-Kincannon model emphasized that UMASR required lesser volume than AnSBR to sustain the same substrate degradation efficacy. Nevertheless, the net gain energy harvested from AnSBR surpassed UMASR by 4.0-folds at HRT of 24-h.
authors.affiliation: Department of Chemistry, Faculty of Science, Alexandria University, P.O. Box 426, Alexandria 21321, Egypt. Electronic address: ama_mostafa@ymail.com.
authors.forename: Alsayed
authors.initials: A
authors.lastname: Mostafa
authors.affiliation: Public Works Engineering Department, Faculty of Engineering, Tanta University, 31521 Tanta City, Egypt.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Elsamadony
authors.affiliation: Department of Chemistry, Faculty of Science, Alexandria University, P.O. Box 426, Alexandria 21321, Egypt.
authors.forename: Ali
authors.initials: A
authors.lastname: El-Dissouky
authors.affiliation: Department of Chemistry, Faculty of Science, Alexandria University, P.O. Box 426, Alexandria 21321, Egypt.
authors.forename: Amel
authors.initials: A
authors.lastname: Elhusseiny
authors.affiliation: Environmental Engineering Department, Egypt-Japan University of Science and Technology (E-JUST), P.O. Box 179, New Borg El Arab City, Alexandria 21934, Egypt; National Research Centre, Water Pollution Research Dept., P.O. 12622, Giza, Egypt. Electronic address: Ahmed.Tawfik@ejust.edu.eg.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Tawfik
mesh_terms: D000693:Anaerobiosis; D001421:Bacteria, Anaerobic / Q000254:growth & development*; D061249:Batch Cell Culture Techniques; D001673:Biodegradation, Environmental; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D002241:Carbohydrates / Q000302:isolation & purification; D005232:Fatty Acids, Volatile / Q000032:analysis; D005285:Fermentation; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008055:Lipids / Q000302:isolation & purification; D055442:Metabolome; D008962:Models, Theoretical; D011506:Proteins / Q000302:isolation & purification; D013997:Time Factors; D062065:Wastewater / Q000382:microbiology*
chemical_list: D002241:Carbohydrates; D005232:Fatty Acids, Volatile; D006859:Hydrogen; D008055:Lipids; D011506:Proteins; D062065:Wastewater
keywords: Anaerobic digestion; Anaerobic sequencing batch reactor; Gelatinaceous wastewater; Net gain energy; Up-flow multi-stage anaerobic sponge reactor