title: Biliary tract external drainage protects against multiple organs injuries of severe acute pancreatitis rats via heme oxygenase-1 upregulation.
abstract: OBJECTIVE
  To investigate the effect of biliary tract external drainage (BTED) on severe acute pancreatitis (SAP) in rats and the relationship with heme oxygenase-1 (HO-1) pathway.
  
  
  METHODS
  Thirty SD rats weighing 250-300 g were randomly assigned into five groups (n = 6): sham surgery (SS) group, SAP group, SAP + BTED group, SAP + zinc protoporphyrin IX (ZnPP) group, SAP + BTED + ZnPP group. The SAP model was induced via retrograde injection of 4% sodium taurocholate (1 mL/kg) into biliopancreatic duct through duodenal wall. BTED was performed by inserting a cannula into the bile duct of SAP rats. Tissue and blood samples were collected 24 h after surgery. Pathological changes in organs were scored. The level of amylase, alanine transaminase (ALT), aspartate aminotransferase (AST), diamine oxidase (DAO), lipopolysaccharide (LPS), myeloperoxidase (MPO) and ability to inhibit hydroxyl radical(·OH) in serum were measured. The expression of hemeoxygenase-1 (HO-1), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in tissues were analyzed by RT- PCR and western-blot.
  
  
  RESULTS
  Organs damage in SAP rats was significantly alleviated by BTED (p < 0.05). Compared to the SAP group, the serum level of amylase, ALT, AST, DAO, MPO, and LPS were significantly lower in the SAP + BTED group, and the ability to inhibit ·OH was significantly higher (p < 0.05). The BETD treatment led to a significant reduction of TNF-α, IL-6 level and a significant increase of HO-1 level in tissues than in SAP rats (p < 0.05). ZnPP significantly inhibited all above mentioned changes.
  
  
  CONCLUSIONS
  BTED protected multiple organs against SAP related injuries via HO-1 upregulation.
authors.affiliation: Department of Emergency Intensive Care Unit, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200025, China. Electronic address: 15152117758@163.com.
authors.forename: Jin-Long
authors.initials: JL
authors.lastname: Wang
authors.affiliation: Department of Emergency Intensive Care Unit, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200025, China. Electronic address: bichatlion@163.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Emergency Intensive Care Unit, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200025, China. Electronic address: mengzaitaxiang@126.com.
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Song
authors.affiliation: Department of Emergency Intensive Care Unit, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200025, China. Electronic address: lumeiling1234@126.com.
authors.forename: Mei-Ling
authors.initials: ML
authors.lastname: Lu
authors.affiliation: Department of Emergency Intensive Care Unit, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200025, China. Electronic address: zhaobing124@163.com.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Department of Emergency Intensive Care Unit, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200025, China. Electronic address: malipostgraduate@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Emergency Intensive Care Unit, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200025, China. Electronic address: rjchenerzhen@163.com.
authors.forename: Er-Zhen
authors.initials: EZ
authors.lastname: Chen
authors.affiliation: Department of Emergency Intensive Care Unit, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200025, China. Electronic address: maoeq@yeah.net.
authors.forename: En-Qiang
authors.initials: EQ
authors.lastname: Mao
mesh_terms: D000818:Animals; D015971:Gene Expression Regulation, Enzymologic; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics / Q000378:metabolism*; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D009102:Multiple Organ Failure; D010195:Pancreatitis / Q000150:complications* / Q000601:surgery*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat
keywords: Biliary tract external drainage; Hemeoxygenase-1; Severe acute pancreatitis