title: Variations of biomarkers response in mussels Mytilus galloprovincialis to low, moderate and high concentrations of organic chemicals and metals.
abstract: The changes of acetylcholinesterase activity (AChE), metallothioneins content (MTs), catalase activity (CAT) and lipid peroxidation (LPO) were assessed after 4 days exposure of mussels Mytilus galloprovincialis to a wide range of sublethal concentrations of chlorpyrifos (CHP, 0.03-100 μg/L), benzo(a)pyrene (B(a)P, 0.01-100 μg/L), cadmium (Cd, 0.2-200 μg/L) and copper (Cu, 0.2-100 μg/L). The activity of AChE in the gills decreased after exposure to CHP and Cu, whereas no change of activity was detected after exposure to B(a)P and Cd. Both induction and decrease of MTs content in digestive gland occurred after exposure to CHP and B(a)P, while a marked increase was evident at highest exposure concentrations of Cd. The content of MTs progressively decreased of MTs with increasing concentration of Cu. CAT activity and LPO in the gills did not change after exposure to any of the chemicals. The results demonstrate different response profile in relation to the type of chemical compound, and highlight the potential implications for evaluation of biological effect of contaminants in marine environment. Furthermore, the AChE activity in the gills and MTs content in the digestive gland could be modulated by CHP and Cu at environmentally relevant concentrations indicating the potential risks of short-term transient mussels exposure that may occur due to run-off from land or accidental releases.
authors.affiliation: Center for Marine Research, Ruđer Bošković Institute, Rovinj, Croatia. Electronic address: Lorena.Peric@irb.hr.
authors.forename: Lorena
authors.initials: L
authors.lastname: Perić
authors.affiliation: Center for Marine Research, Ruđer Bošković Institute, Rovinj, Croatia.
authors.forename: Vedrana
authors.initials: V
authors.lastname: Nerlović
authors.affiliation: Teaching Institute of Public Health of Primorsko-goranska County, Rijeka, Croatia.
authors.forename: Paula
authors.initials: P
authors.lastname: Žurga
authors.affiliation: Marine Sciences, Juraj Dobrila University of Pula, Pula, Croatia.
authors.forename: Luka
authors.initials: L
authors.lastname: Žilić
authors.affiliation: National Institute of Biology, Marine Biology Station Piran, Piran, Slovenia.
authors.forename: Andreja
authors.initials: A
authors.lastname: Ramšak
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity; D015415:Biomarkers / Q000378:metabolism; D002104:Cadmium / Q000633:toxicity; D002374:Catalase / Q000378:metabolism; D004390:Chlorpyrifos / Q000633:toxicity; D003300:Copper / Q000633:toxicity; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000378:metabolism; D005880:Gills / Q000187:drug effects / Q000378:metabolism; D007306:Insecticides / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects; D008668:Metallothionein / Q000378:metabolism; D049878:Mytilus / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D007306:Insecticides; D014874:Water Pollutants, Chemical; D002104:Cadmium; D001564:Benzo(a)pyrene; D003300:Copper; D008668:Metallothionein; D002374:Catalase; D000110:Acetylcholinesterase; D004390:Chlorpyrifos
keywords: Acetylcholinesterase; Catalase; Contaminants; Lipid peroxidation; Metallothioneins; Mytilus galloprovincialis