title: Incidence and biodiversity of yeasts, dermatophytes and non-dermatophytes in superficial skin infections in Assiut, Egypt.
abstract: OBJECTIVE
  The aim was to identify the incidence of the causal agents from dermatophytes, non-dermatophytes and yeasts in Assiut Governorate employing, beside the morphological and physiological techniques, the genotypic ones.
  
  
  METHODS
  Samples from infected nails, skin and hair were taken from 125 patients.
  
  
  METHODS
  Patients who presented with onychomycosis, tinea capitis, tinea corporis, tinea cruris and tinea pedis during the period from February 2012 to October 2015 were clinically examined and diagnosed by dermatologists and were guided to Assiut University Mycological Centre for direct microscopic examination, culturing and identification.
  
  
  RESULTS
  Onychomycosis was the most common infecting (64.8% of the cases) followed by tinea capitis (17.6%). Direct microscopic preparations showed only 45 positive cases, while 96 cases showed positive cultures. Infections were more frequent in females than males. Fifty-one fungal species and 1 variety were obtained. Yeasts were the main agents being cultured from 46.02% of total cases. Non-dermatophytes were the second etiologic agents. Aspergillus was responsible for infecting 19.47% of total cases and dermatophytes appeared in only 15.93% of the cases.
  
  
  CONCLUSIONS
  Yeasts were the main causal agents followed by non-dermatophytic fungi (mainly species of Aspergillus, then Alternaria, Scopulariopsis and Fusarium). Both direct microscopic preparations and culturing are recommended for mycological evaluation of clinical specimens. Sequence analysis of ITS region is recommended for yeast identification.
authors.affiliation: Department of Botany and Microbiology, Faculty of Science, Assiut University Mycological Centre, Assiut University, Assiut, Egypt.
authors.forename: A H
authors.initials: AH
authors.lastname: Moubasher
authors.affiliation: Department of Botany and Microbiology, Faculty of Science, Assiut University Mycological Centre, Assiut University, Assiut, Egypt. Electronic address: masater59@yahoo.com.
authors.forename: M A
authors.initials: MA
authors.lastname: Abdel-Sater
authors.affiliation: Department of Botany and Microbiology, Faculty of Science, Assiut University Mycological Centre, Assiut University, Assiut, Egypt.
authors.forename: Z
authors.initials: Z
authors.lastname: Soliman
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003883:Arthrodermataceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D044822:Biodiversity; D002648:Child; D002675:Child, Preschool; D003872:Dermatitis / Q000453:epidemiology / Q000382:microbiology; D003881:Dermatomycoses / Q000453:epidemiology* / Q000382:microbiology*; D004534:Egypt / Q000453:epidemiology; D005260:Female; D006801:Humans; D015994:Incidence; D007223:Infant; D008297:Male; D008875:Middle Aged; D016533:Mycological Typing Techniques; D012867:Skin / Q000382:microbiology*; D015003:Yeasts / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D055815:Young Adult
keywords: Dermatophytes et non dermatophytes; Dermatophytic; Levures; Mycoses superficielles; Non-dermatophytic; PCR; Skin infections; Yeasts