title: Gas chromatography-mass spectrometry analysis of effects of dietary fish oil on total fatty acid composition in mouse skin.
abstract: Altering the fatty acid (FA) composition in the skin by dietary fish oil could provide therapeutic benefits. Although it has been shown that fish oil supplementation enhances EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid) abundance in the skin, comprehensive skin FA profiling is needed. We established a gas chromatography-mass spectrometry method, which allows precise quantification of FA profile using small (<24 mm2for mice and <12 mm2for humans) skin specimens that can be readily obtained from live mice and humans. We determined mouse skin FA composition after 2, 4 and 8 weeks of consuming a control diet or a diet supplemented with fish oil. Fish oil markedly enhanced EPA and DHA in mouse skin within 2 weeks, and this increase plateaued after 4 weeks. The FA composition in mouse skin was different from that of serum, indicating that skin has homeostatic control of FA metabolism. Mice fed the control diet designed to simulate Western human diet displayed similar skin FA composition as that of humans. The present study presents a validated method for FA quantification that is needed to investigate the mechanisms of actions of dietary treatments in both mouse and human skin.
authors.affiliation: Department of Dermatology, University of Michigan, Ann Arbor, MI, USA.|Department of photomedicine, Shanghai Dermatology Hospital, China.
authors.forename: Peiru
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Dermatology, University of Michigan, Ann Arbor, MI, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Sun
authors.affiliation: Department of Family Medicine, University of Michigan, Ann Arbor, MI, USA.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Ren
authors.affiliation: Department of Family Medicine, University of Michigan, Ann Arbor, MI, USA.
authors.forename: Zora
authors.initials: Z
authors.lastname: Djuric
authors.affiliation: Department of Dermatology, University of Michigan, Ann Arbor, MI, USA.
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Fisher
authors.affiliation: Department of photomedicine, Shanghai Dermatology Hospital, China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Dermatology, University of Michigan, Ann Arbor, MI, USA.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D019587:Dietary Supplements; D004281:Docosahexaenoic Acids / Q000378:metabolism; D015118:Eicosapentaenoic Acid / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis* / Q000378:metabolism*; D015525:Fatty Acids, Omega-3 / Q000378:metabolism; D005395:Fish Oils / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D050356:Lipid Metabolism / Q000187:drug effects; D051379:Mice; D012867:Skin / Q000187:drug effects* / Q000378:metabolism*; D013997:Time Factors
chemical_list: D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid