title: Antioxidant and cytoprotective effects of morin against hydrogen peroxide-induced oxidative stress are associated with the induction of Nrf-2‑mediated HO-1 expression in V79-4 Chinese hamster lung fibroblasts.
abstract: Natural phytochemicals of plant origin, including flavonoids, have been found to be potent antioxidants providing beneficial effects against oxidative stress-related diseases. The present study was carried out to investigate the antioxidant properties of morin, a flavonoid originally isolated from the flowering plants of the Moraceae family. Superoxide dismutase (SOD)‑like activity and 2,2'‑azino‑bis‑(3‑ethylbenzothiazoline‑6‑sulfonic acid) (ABTS•+) radical scavenging activity were determined. We also investigated the cytoprotective effects of morin against hydrogen peroxide (H2O2)‑induced DNA damage and apoptosis in V79‑4 Chinese hamster lung fibroblasts. Our results demonstrated that morin had strong scavenging effects against ABTS•+ radicals with enhanced SOD activity, which varied in a dose-dependent manner. Morin was found to reduce H2O2‑induced intracellular reactive oxygen species generation and nuclear DNA damage, and it recovered cell viability damaged by H2O2 via inhibition of mitochondrial dysfunction‑mediated apoptosis. Notably, the treatment of V79‑4 cells with morin markedly enhanced the expression of heme oxygenase‑1 (HO‑1) but not quinone oxidoreductase-1, which was associated with the increased expression and phosphorylation of nuclear factor-erythroid 2-related factor 2 (Nrf2) and the downregulation of Kelch‑like ECH‑associated protein 1 expression. Based on our findings, we conclude that morin effectively ameliorated oxidative stress‑induced DNA damage through intrinsic free radical scavenging activity and activation of the Nrf2/HO-1 pathway.
authors.affiliation: Anti‑Aging Research Center, Dongeui University, Busan 614‑714, Republic of Korea.
authors.forename: Moon Hee
authors.initials: MH
authors.lastname: Lee
authors.affiliation: Department of Parasitology and Genetics, Kosin University, College of Medicine, Busan 602‑702, Republic of Korea.
authors.forename: Hee-Jae
authors.initials: HJ
authors.lastname: Cha
authors.affiliation: Anti‑Aging Research Center, Dongeui University, Busan 614‑714, Republic of Korea.
authors.forename: Eun Ok
authors.initials: EO
authors.lastname: Choi
authors.affiliation: Natural Products Research Team, Marine Biodiversity Institute of Korea, Seocheon 325‑902, Republic of Korea.
authors.forename: Min Ho
authors.initials: MH
authors.lastname: Han
authors.affiliation: Department of Food Science and Biotechnology, College of Engineering, Kyungsung University, Busan 608‑736, Republic of Korea.
authors.forename: Sung Ok
authors.initials: SO
authors.lastname: Kim
authors.affiliation: Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju 690‑756, Republic of Korea.
authors.forename: Gi-Young
authors.initials: GY
authors.lastname: Kim
authors.affiliation: Department of Biochemistry, Dongeui University College of Korean Medicine, Busan 614‑052, Republic of Korea.
authors.forename: Su Hyun
authors.initials: SH
authors.lastname: Hong
authors.affiliation: Department of Molecular Biology, College of Natural Sciences and Human Ecology, Dongeui University, Busan 614‑714, Republic of Korea.
authors.forename: Cheol
authors.initials: C
authors.lastname: Park
authors.affiliation: School of Food Science and Technology, Chung‑Ang University, Anseong 456‑756, Republic of Korea.
authors.forename: Sung-Kwon
authors.initials: SK
authors.lastname: Moon
authors.affiliation: Department of Dental Hygiene, College of Health Sciences, Youngsan University, Yangsan 626‑790, Republic of Korea.
authors.forename: Soon-Jeong
authors.initials: SJ
authors.lastname: Jeong
authors.affiliation: Department of Oral Histology and Developmental Biology, School of Dentistry, Chosun University, Gwangju 501‑759, Republic of Korea.
authors.forename: Moon-Jin
authors.initials: MJ
authors.lastname: Jeong
authors.affiliation: Department of Urology, Chungbuk National University, College of Medicine and Institute for Tumor Research, Cheongju 362‑763, Republic of Korea.
authors.forename: Wun-Jae
authors.initials: WJ
authors.lastname: Kim
authors.affiliation: Anti‑Aging Research Center, Dongeui University, Busan 614‑714, Republic of Korea.
authors.forename: Yung Hyun
authors.initials: YH
authors.lastname: Choi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003412:Cricetulus; D019610:Cytoprotection / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D005347:Fibroblasts; D005419:Flavonoids / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D051547:Heme Oxygenase-1 / Q000235:genetics* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051267:NF-E2-Related Factor 2 / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D047309:Flavones
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D051547:Heme Oxygenase-1; D006861:Hydrogen Peroxide; D051267:NF-E2-Related Factor 2; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; C008548:morin; D047309:Flavones