title: Deciphering tumor heterogeneity from FFPE tissues: Its promise and challenges.
abstract: An impediment to the understanding of cancer is the heterogeneous nature of cell populations within a tumor microenvironment. We reported a method to query protein signaling in single epithelial cells from formalin-fixed paraffin-embedded (FFPE) colorectal cancer tissues. Here, we discuss the feasibility and limitations of this approach for investigating signaling state heterogeneity.
authors.affiliation: Epithelial Biology Center and Department of Cell and Developmental Biology, Vanderbilt University School of Medicine , Nashville, TN, USA.
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Simmons
authors.affiliation: Epithelial Biology Center and Department of Cell and Developmental Biology, Vanderbilt University School of Medicine , Nashville, TN, USA.
authors.forename: Ken S
authors.initials: KS
authors.lastname: Lau
keywords: Colorectal cancer; FFPE; disaggregation; epithelial cells; mass cytometry; signal transduction; single cell; solid tissues; systems biology; tumor heterogeneity