title: Structure and polymorphisms of the major histocompatibility complex in the Oriental stork, Ciconia boyciana.
abstract: The major histocompatibility complex (MHC) is highly polymorphic and plays a central role in the vertebrate immune system. Despite its functional consistency, the MHC genomic structure differs substantially among organisms. In birds, the MHCs of Galliformes and the Japanese crested ibis (Pelecaniformes) are well-characterized, but information about other avian MHCs remains scarce. The Oriental stork (Ciconia boyciana, order Ciconiiformes) is a large endangered migrant. The current Japanese population of this bird originates from a few founders; thus, understanding the genetic diversity among them is critical for effective population management. We report the structure and polymorphisms in C. boyciana MHC. One contig (approximately 128 kb) was assembled by screening of lambda phage genomic library and its complete sequence was determined, revealing a gene order of COL11A2, two copies of MHC-IIA/IIB pairs, BRD2, DMA/B1/B2, MHC-I, TAP1/2, and two copies each of pseudo MHC-I and TNXB. This structure was highly similar to that of the Japanese crested ibis, but largely different from that of Galliformes, at both the terminal regions. Genotyping of the MHC-II region detected 10 haplotypes among the six founders. These results provide valuable insights for future studies on the evolution of the avian MHCs and for conservation of C. boyciana.
authors.affiliation: Laboratory of Animal Breeding and Genetics, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Tsuji
authors.affiliation: Laboratory of Animal Breeding and Genetics, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
authors.forename: Yukio
authors.initials: Y
authors.lastname: Taniguchi
authors.affiliation: Laboratory of Animal Breeding and Genetics, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
authors.forename: Shintaro
authors.initials: S
authors.lastname: Ishizuka
authors.affiliation: Laboratory of Animal Breeding and Genetics, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Matsuda
authors.affiliation: Department of Agrobiology, Faculty of Agriculture, Niigata University, Niigata, Japan.
authors.forename: Takahisa
authors.initials: T
authors.lastname: Yamada
authors.affiliation: Graduate School of Regional Resource Management, University of Hyogo, Toyooka, Japan.|Hyogo Park of the Oriental White Stork, Toyooka, Japan.
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Naito
authors.affiliation: Laboratory of Animal Breeding and Genetics, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.|Institute for Research Promotion, Center for Toki and Ecological Restoration, Niigata University, Niigata, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Iwaisaki
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics; D001717:Birds / Q000235:genetics*; D056727:Endangered Species; D019143:Evolution, Molecular; D018703:Founder Effect; D023061:Gene Order; D007564:Japan; D008285:Major Histocompatibility Complex / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D030161:Avian Proteins