title: Durable vesicles for reconstitution of membrane proteins in biotechnology.
abstract: The application of membrane proteins in biotechnology requires robust, durable reconstitution systems that enhance their stability and support their functionality in a range of working environments. Vesicular architectures are highly desirable to provide the compartmentalisation to utilise the functional transmembrane transport and signalling properties of membrane proteins. Proteoliposomes provide a native-like membrane environment to support membrane protein function, but can lack the required chemical and physical stability. Amphiphilic block copolymers can also self-assemble into polymersomes: tough vesicles with improved stability compared with liposomes. This review discusses the reconstitution of membrane proteins into polymersomes and the more recent development of hybrid vesicles, which blend the robust nature of block copolymers with the biofunctionality of lipids. These novel synthetic vesicles hold great promise for enabling membrane proteins within biotechnologies by supporting their enhancedin vitroperformance and could also contribute to fundamental biochemical and biophysical research by improving the stability of membrane proteins that are challenging to work with.
authors.affiliation: School of Chemistry and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K. p.a.beales@leeds.ac.uk.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Beales
authors.affiliation: School of Chemistry and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K.
authors.forename: Sanobar
authors.initials: S
authors.lastname: Khan
authors.affiliation: School of Biomedical Sciences and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K.
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Muench
authors.affiliation: School of Biomedical Sciences and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K.
authors.forename: Lars J C
authors.initials: LJ
authors.lastname: Jeuken
mesh_terms: D001692:Biological Transport; D001709:Biotechnology / Q000379:methods* / Q000639:trends; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D008956:Models, Chemical; D008958:Models, Molecular; D011108:Polymers / Q000737:chemistry / Q000378:metabolism; D011510:Proteolipids / Q000737:chemistry* / Q000378:metabolism; D053835:Unilamellar Liposomes / Q000737:chemistry* / Q000378:metabolism
chemical_list: D008051:Lipid Bilayers; D008563:Membrane Lipids; D008565:Membrane Proteins; D011108:Polymers; D011510:Proteolipids; D053835:Unilamellar Liposomes; C020485:proteoliposomes
keywords: bionanotechnology; block copolymers; in vitro reconstitution; membrane proteins; synthetic biology; vesicles