title: Application of fluorescence polarization immunoassay for determination of carbamazepine in wastewater.
abstract: Carbamazepine is an antiepileptic drug that can be used as a marker for the cleaning efficiency of wastewater treatment plants. Here, we present the optimization of a fast and easy on-site measurement system based on fluorescence polarization immunoassay and the successful application to wastewater. A new monoclonal highly specific anti-carbamazepine antibody was applied. The automated assay procedure takes 16 min and does not require sample preparation besides filtration. The recovery rates for carbamazepine in wastewater samples were between 60.8 and 104% with good intra- and inter-assay coefficients of variations (less than 15 and 10%, respectively). This automated assay enables for the on-site measurement of carbamazepine in wastewater treatment plants.
authors.affiliation: Bundesanstalt für Materialforschung und -prüfung (BAM), 12205 Berlin, Germany; Institute of Bioanalytics, Department of Biotechnology, Technische Universität Berlin, Seestr. 13, 13353 Berlin, Germany.
authors.forename: Lidia
authors.initials: L
authors.lastname: Oberleitner
authors.affiliation: aokin AG, Robert-Rössle-Str. 10, 13125 Berlin, Germany.
authors.forename: Ursula
authors.initials: U
authors.lastname: Dahmen-Levison
authors.affiliation: Institute of Bioanalytics, Department of Biotechnology, Technische Universität Berlin, Seestr. 13, 13353 Berlin, Germany.
authors.forename: Leif-Alexander
authors.initials: LA
authors.lastname: Garbe
authors.affiliation: Bundesanstalt für Materialforschung und -prüfung (BAM), 12205 Berlin, Germany. Electronic address: rudolf.schneider@bam.de.
authors.forename: Rudolf J
authors.initials: RJ
authors.lastname: Schneider
mesh_terms: D000927:Anticonvulsants; D001681:Biological Assay; D002220:Carbamazepine; D016231:Fluorescence Polarization Immunoassay; D062065:Wastewater
chemical_list: D000927:Anticonvulsants; D002220:Carbamazepine; D062065:Wastewater
keywords: Assay optimization; Fluorescence polarization immunoassay; Mix-and-read assay; On-site measurement; Wastewater treatment plant