title: Comparison of biodistribution profile of monoclonal antibodies nanoparticles and aptamers in rats with breast cancer.
abstract: The use of monoclonal antibodies and aptamers is growing every single day, as the use of nanoparticle systems. Although most of the products are under investigation, there are a few commercialized products available at the market, for human consume. In this study, we have compared three formulations (aptamer anti-MUC1, monoclonal antibody - Trastuzumab and monoclonal antibodies nanoparticles - PLA/PVA/MMT trastuzumab) to identify their profile as also to understand their behavior into an alive biological system. In this direction the radiolabeling of the products were done and they were all tested in animals (in vivo) in two conditions: healthy rats and breast cancer induced animals. The results showed that the nanoparticle has the better biodistribution profile, followed by the aptamer. We conclude that more studies and a global effort to elucidate the biological behavior of drugs and especially nano-drugs are necessary.
authors.affiliation: a Rio de Janeiro Federal University, Eloisa Mano Macromolecules Institute , Rio de Janeiro, Brazil.
authors.forename: Cristal
authors.initials: C
authors.lastname: Cerqueira-Coutinho
authors.affiliation: b Laboratory of Research in Pharmaceutical Sciences, Zona Oeste State University , Rio de Janeiro , Brazil.
authors.forename: Sotiris
authors.initials: S
authors.lastname: Missailidis
authors.affiliation: b Laboratory of Research in Pharmaceutical Sciences, Zona Oeste State University , Rio de Janeiro , Brazil.|c Graduate Program in Public Health and Environment National School of Public Health, Oswaldo Cruz Foundation , Rio de Janeiro, Brazil.
authors.forename: Jéssica
authors.initials: J
authors.lastname: Alessandra-Perini
authors.affiliation: b Laboratory of Research in Pharmaceutical Sciences, Zona Oeste State University , Rio de Janeiro , Brazil.
authors.forename: Daniel Escorsim
authors.initials: DE
authors.lastname: Machado
authors.affiliation: b Laboratory of Research in Pharmaceutical Sciences, Zona Oeste State University , Rio de Janeiro , Brazil.|c Graduate Program in Public Health and Environment National School of Public Health, Oswaldo Cruz Foundation , Rio de Janeiro, Brazil.|d National Institute of Traumatology and Orthopedics , Rio de Janeiro, Brazil.
authors.forename: Jamila Alessandra
authors.initials: JA
authors.lastname: Perini
authors.affiliation: e Laboratory of Radiopharmacy and Nanoradiopharmaceuticals, Zona Oeste State University , Rio de Janeiro, Brazil.
authors.forename: Ralph
authors.initials: R
authors.lastname: Santos-Oliveira
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000493:pharmacokinetics*; D052158:Aptamers, Peptide / Q000737:chemistry / Q000493:pharmacokinetics*; D005260:Female; D006801:Humans; D008321:Mammary Glands, Animal / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology; D008325:Mammary Neoplasms, Experimental / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology; D018396:Mucin-1 / Q000737:chemistry / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000737:chemistry; D011877:Radionuclide Imaging / Q000379:methods*; D019275:Radiopharmaceuticals / Q000737:chemistry / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling / Q000379:methods; D013667:Technetium / Q000737:chemistry / Q000493:pharmacokinetics; D014018:Tissue Distribution; D000068878:Trastuzumab / Q000737:chemistry / Q000493:pharmacokinetics*
chemical_list: D000970:Antineoplastic Agents; D052158:Aptamers, Peptide; C506177:MUC1 protein, rat; D018396:Mucin-1; D011091:Polyesters; D019275:Radiopharmaceuticals; C033616:poly(lactide); D013667:Technetium; D011142:Polyvinyl Alcohol; D000068878:Trastuzumab
keywords: Aptamers; nano-radiopharmaceuticals; nanoparticles; radiopharmaceuticals