title: Comparison of culture, single and multiplex real-time PCR for detection of Sabin poliovirus shedding in recently vaccinated Indian children.
abstract: Although, culture is considered the gold standard for poliovirus detection from stool samples, real-time PCR has emerged as a faster and more sensitive alternative. Detection of poliovirus from the stool of recently vaccinated children by culture, single and multiplex real-time PCR was compared. Of the 80 samples tested, 55 (68.75%) were positive by culture compared to 61 (76.25%) and 60 (75%) samples by the single and one step multiplex real-time PCR assays respectively. Real-time PCR (singleplex and multiplex) is more sensitive than culture for poliovirus detection in stool, although the difference was not statistically significant.
authors.affiliation: Department of Gastrointestinal Sciences, Christian Medical College, Vellore, India.
authors.forename: Sidhartha
authors.identifier: 0000-0003-3927-7392
authors.initials: S
authors.lastname: Giri
authors.affiliation: Department of Clinical Virology, Christian Medical College, Vellore, India.
authors.forename: Anand K
authors.initials: AK
authors.lastname: Rajan
authors.affiliation: Department of Gastrointestinal Sciences, Christian Medical College, Vellore, India.
authors.forename: Nirmal
authors.initials: N
authors.lastname: Kumar
authors.affiliation: Department of Gastrointestinal Sciences, Christian Medical College, Vellore, India.
authors.forename: Pavithra
authors.initials: P
authors.lastname: Dhanapal
authors.affiliation: Department of Gastrointestinal Sciences, Christian Medical College, Vellore, India.
authors.forename: Jayalakshmi
authors.initials: J
authors.lastname: Venkatesan
authors.affiliation: Institute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom.
authors.forename: Miren
authors.initials: M
authors.lastname: Iturriza-Gomara
authors.affiliation: Division of Infectious Diseases and International Health, University of Virginia School of Medicine, Charlottesville, Virginia.
authors.forename: Mami
authors.initials: M
authors.lastname: Taniuchi
authors.affiliation: Department of Community Medicine, Christian Medical College, Vellore, India.
authors.forename: Jacob
authors.initials: J
authors.lastname: John
authors.affiliation: Department of Clinical Virology, Christian Medical College, Vellore, India.
authors.forename: Asha Mary
authors.initials: AM
authors.lastname: Abraham
authors.affiliation: Department of Gastrointestinal Sciences, Christian Medical College, Vellore, India.
authors.forename: Gagandeep
authors.initials: G
authors.lastname: Kang
mesh_terms: D005243:Feces / Q000821:virology*; D005260:Female; D006801:Humans; D007194:India; D007223:Infant; D008297:Male; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D017955:Poliovirus / Q000302:isolation & purification*; D011055:Poliovirus Vaccine, Oral / Q000008:administration & dosage*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014776:Virus Cultivation / Q000379:methods*; D017201:Virus Shedding
chemical_list: D011055:Poliovirus Vaccine, Oral
keywords: cell culture; poliovirus; shedding