title: Leukemia microvesicles affect healthy hematopoietic stem cells.
abstract: Microvesicles are released by different cell types and shuttle mRNAs and microRNAs which have the possibility to transfer genetic information to a target cell and alter its function. Acute myeloid leukemia is a malignant disorder, and leukemic cells occupy all the bone marrow microenvironment. In this study, we investigate the effect of leukemia microvesicles on healthy umbilical cord blood hematopoietic stem cells to find evidence of cell information transferring. Leukemia microvesicles were isolated from acute myeloid leukemia patients and were co-incubated with healthy hematopoietic stem cells. After 7 days, cell count, hematopoietic stem cell-specific cluster of differentiation (CD) markers, colony-forming unit assay, and some microRNA gene expressions were assessed. Data showed a higher number of hematopoietic stem cells after being treated with leukemia microvesicles compared with control (treated with no microvesicles) and normal (treated with normal microvesicles) groups. Also, increased levels of microRNA-21 and microRNA-29a genes were observed in this group, while colony-forming ability was still maintained and high ranges of CD34+, CD34+CD38-, CD90+, and CD117+phenotypes were observed as stemness signs. Our results suggest that leukemia microvesicles are able to induce some effects on healthy hematopoietic stem cells such as promoting cell survival and some microRNAs deregulation, while stemness is maintained.
authors.affiliation: 1 Department of Hematology, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran.
authors.forename: Farnaz
authors.initials: F
authors.lastname: Razmkhah
authors.affiliation: 1 Department of Hematology, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
authors.affiliation: 2 Stem cell technology research center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Davood
authors.initials: D
authors.lastname: Mehrabani
authors.affiliation: 3 Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Karimi
authors.affiliation: 4 Blood Transfusion Research Center, Department of Pathology, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
authors.forename: Sedigheh
authors.initials: S
authors.lastname: Amini Kafi-Abad
authors.affiliation: 5 Hematology research center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mani
authors.initials: M
authors.lastname: Ramzi
authors.affiliation: 3 Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.|6 Department of Hematology-Oncology and Stem Cell Transplantation, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mahdiyar
authors.initials: M
authors.lastname: Iravani Saadi
authors.affiliation: 7 Atieh Hospital, Tehran, Iran.
authors.forename: Javad
authors.initials: J
authors.lastname: Kakoui
mesh_terms: D000368:Aged; D001854:Bone Marrow Cells / Q000473:pathology; D016022:Case-Control Studies; D055252:Cell-Derived Microparticles / Q000473:pathology*; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D008297:Male; D008875:Middle Aged
keywords: Microvesicles; hematopoietic stem cell; leukemia; transformation