title: Effect of BMP-2 Delivery Mode on Osteogenic Differentiation of Stem Cells.
abstract: Differentiation of stem cells is an important strategy for regeneration of defective tissue in stem cell therapy. Bone morphogenetic protein-2 (BMP-2) is a well-known osteogenic differentiation factor that stimulates stem cell signaling pathways by activating transmembrane type I and type II receptors. However, BMPs have a very short half-life and may rapidly lose their bioactivity. Thus, a BMP delivery system is required to take advantage of an osteoinductive effect for osteogenic differentiation. Previously, BMP delivery has been designed and evaluated for osteogenic differentiation, focusing on carriers and sustained release system for delivery of BMPs. The effect of the delivery mode in cell culture plate on osteogenic differentiation potential was not evaluated. Herein, to investigate the effect of delivery mode on osteogenic differentiation of BM-MSCs in this study, we fabricated bottom-up release and top-down release systems for culture plate delivery of BMP-2. And also, we selected Arg-Gly-Asp- (RGD-) conjugated alginate hydrogel for BMP-2 delivery because alginate is able to release BMP-2 in a sustained manner and it is a biocompatible material. After 7 days of culture, the bottom-up release system in culture plate significantly stimulated alkaline phosphate activity of human bone marrow-mesenchymal stem cells. The present study highlights the potential value of the tool in stem cell therapy.
authors.affiliation: Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul, Republic of Korea.
authors.forename: Taekhee
authors.identifier: 0000-0001-9958-9297
authors.initials: T
authors.lastname: Jung
authors.affiliation: Department of Chemical Engineering, Hanyang University, Seoul, Republic of Korea.
authors.forename: John Hwan
authors.identifier: 0000-0003-3395-5797
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul, Republic of Korea.
authors.forename: Soonjung
authors.identifier: 0000-0002-9384-6034
authors.initials: S
authors.lastname: Park
authors.affiliation: AmorePacific Corp./R&D Center, Yongin-si, Gyeonggi-do, Republic of Korea.
authors.forename: Yong-Jin
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul, Republic of Korea.
authors.forename: Joseph
authors.identifier: 0000-0003-1143-3699
authors.initials: J
authors.lastname: Seo
authors.affiliation: Predictive Model Research Center, Korea Institute of Toxicology, Daejeon, Republic of Korea.
authors.forename: Hye-Eun
authors.identifier: 0000-0002-3425-6333
authors.initials: HE
authors.lastname: Shim
authors.affiliation: Predictive Model Research Center, Korea Institute of Toxicology, Daejeon, Republic of Korea; Department of Human and Environmental Toxicology, University of Science and Technology, Daejeon, Republic of Korea.
authors.forename: Ki-Suk
authors.initials: KS
authors.lastname: Kim
authors.affiliation: Department of Dentistry, Korea University, Ansan Hospital, Ansan, Republic of Korea.
authors.forename: Hyon-Seok
authors.initials: HS
authors.lastname: Jang
authors.affiliation: Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul, Republic of Korea.
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Chung
authors.affiliation: Department of Chemical Engineering, Hanyang University, Seoul, Republic of Korea.
authors.forename: Seong-Geun
authors.identifier: 0000-0003-1276-3382
authors.initials: SG
authors.lastname: Oh
authors.affiliation: Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul, Republic of Korea.
authors.forename: Sung-Hwan
authors.identifier: 0000-0002-7750-0818
authors.initials: SH
authors.lastname: Moon
authors.affiliation: Predictive Model Research Center, Korea Institute of Toxicology, Daejeon, Republic of Korea; Department of Human and Environmental Toxicology, University of Science and Technology, Daejeon, Republic of Korea.
authors.forename: Sun-Woong
authors.identifier: 0000-0001-8098-2144
authors.initials: SW
authors.lastname: Kang