title: Achieving short-cut nitrification and denitrification in modified intermittently aerated constructed wetland.
abstract: This study aim to enhance nitrogen removal performance via shifting nitrogen removal pathway from nitrate to nitrite pathway. It was demonstrated that nitrite pathway was successfully and stably achieved in CWs by using modified intermittent aeration control with aeration 20min/non-aeration 100min and reducing DO concentration during aeration, nitrite in the effluent could accumulate to over 70% of the total oxidized nitrogen. Q-PCR analysis showed that nitrifying microbial communities were optimized under the alternating anoxic and aerobic conditions, ammonia oxidizing bacteria increased from 7.15×106to 8.99×106copies/g, while nitrite oxidizing bacteria decreased approximately threefold after 234days operation. Most importantly, high nitrogen removal efficiency with ammonium removal efficiency of 94.6%, and total nitrogen removal efficiency of 82.6% could be achieved via nitrite pathway even under carbon limiting conditions. In comparison to the nitrate pathway, the nitrite pathway could improve the TN removal by about 55%.
authors.affiliation: College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Hou
authors.affiliation: School of Resources and Environmental Engineering, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Xia
authors.affiliation: College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Ma
authors.affiliation: College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Yiqing
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: The State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan, Hubei, China.
authors.forename: Yiyong
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China. Electronic address: xgh@mail.hzau.edu.cn.
authors.forename: Xugang
authors.initials: X
authors.lastname: He
mesh_terms: D000332:Aerobiosis; D064751:Ammonium Compounds / Q000378:metabolism; D001419:Bacteria / Q000378:metabolism; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology; D058440:Denitrification; D004777:Environment; D009566:Nitrates / Q000378:metabolism; D058465:Nitrification; D009573:Nitrites / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D010084:Oxidation-Reduction; D016133:Polymerase Chain Reaction; D053833:Wetlands
chemical_list: D064751:Ammonium Compounds; D009566:Nitrates; D009573:Nitrites; D009584:Nitrogen
keywords: Intermittent aeration control; Nitrogen removal pathway; Partial nitrification; Vertical flow constructed wetland