title: A Phenylselenium-Substituted BODIPY Fluorescent Turn-off Probe for Fluorescence Imaging of Hydrogen Sulfide in Living Cells.
abstract: Herein a phenylselenium-substituted BODIPY (1) fluorescent turn-off sensor was developed for the purpose to achieve excellent selectivity and sensitivity for H2S detection based on the substitution reaction of the phenylselenide group at the 3-position with H2S. The excess addition of hydrogen sulfide promoted further substitution of the phenylselenide group at the 5-position of the probe and was accompanied by a further decrease in fluorescence emission intensity. Sensor 1 demonstrated remarkable performance with 49-fold red color fluorescence intensity decrease at longer excitation wavelength, a low detection limit (0.0025 μM), and specific fluorescent response toward H2S over anions, biothiols, and other amino acids in neutral media. It showed no obvious cell toxicity and good membrane permeability, which was well exploited for intracellular H2S detection and imaging through fluorescence microscopy imaging.
authors.affiliation: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University , Lanzhou 730000, P. R. China.
authors.forename: Deyan
authors.initials: D
authors.lastname: Gong
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Xujiaping 1, Lanzhou, Gansu Province 730046, P. R. China.
authors.forename: Xiangtao
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Institute of Urology, The Second Hospital of Lanzhou University , Lanzhou, Gansu Province, P. R. China.
authors.forename: Yuejun
authors.initials: Y
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Xujiaping 1, Lanzhou, Gansu Province 730046, P. R. China.
authors.forename: Shi-Chong
authors.initials: SC
authors.lastname: Han
authors.affiliation: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University , Lanzhou 730000, P. R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Deng
authors.affiliation: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University , Lanzhou 730000, P. R. China.
authors.forename: Anam
authors.initials: A
authors.lastname: Iqbal
authors.affiliation: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University , Lanzhou 730000, P. R. China.
authors.forename: Weisheng
authors.initials: W
authors.lastname: Liu
authors.affiliation: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University , Lanzhou 730000, P. R. China.
authors.forename: Wenwu
authors.identifier: 0000-0002-9782-6647
authors.initials: W
authors.lastname: Qin
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Xujiaping 1, Lanzhou, Gansu Province 730046, P. R. China.
authors.forename: Huichen
authors.initials: H
authors.lastname: Guo
mesh_terms: D000818:Animals; D001896:Boron Compounds / Q000737:chemistry*; D002460:Cell Line; D002463:Cell Membrane Permeability; D006224:Cricetinae; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D006862:Hydrogen Sulfide / Q000032:analysis* / Q000737:chemistry; D008856:Microscopy, Fluorescence; D012643:Selenium / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D012643:Selenium; D006862:Hydrogen Sulfide