title: β-Adrenoceptor activation enhances L-type calcium channel currents in anterior piriform cortex pyramidal cells of neonatal mice: implication for odor learning.
abstract: Early odor preference learning occurs in one-week-old rodents when a novel odor is paired with a tactile stimulation mimicking maternal care. β-Adrenoceptors and L-type calcium channels (LTCCs) in the anterior piriform cortex (aPC) are critically involved in this learning. However, whether β-adrenoceptors interact directly with LTCCs in aPC pyramidal cells is unknown. Here we show that pyramidal cells expressed significant LTCC currents that declined with age. β-Adrenoceptor activation via isoproterenol age-dependently enhanced LTCC currents. Nifedipine-sensitive, isoproterenol enhancement of calcium currents was only observed in post-natal day 7-10 mice. APC β-adrenoceptor activation induced early odor preference learning was blocked by nifedipine coinfusion.
authors.affiliation: Biomedical Sciences, Faculty of Medicine, Memorial University of Newfoundland, St. John's, Newfoundland and Labrador A1B 3V6, Canada.
authors.forename: Abhinaba
authors.initials: A
authors.lastname: Ghosh
authors.affiliation: Biomedical Sciences, Faculty of Medicine, Memorial University of Newfoundland, St. John's, Newfoundland and Labrador A1B 3V6, Canada.
authors.forename: Bandhan
authors.initials: B
authors.lastname: Mukherjee
authors.affiliation: Biomedical Sciences, Faculty of Medicine, Memorial University of Newfoundland, St. John's, Newfoundland and Labrador A1B 3V6, Canada.
authors.forename: Xihua
authors.initials: X
authors.lastname: Chen
authors.affiliation: Biomedical Sciences, Faculty of Medicine, Memorial University of Newfoundland, St. John's, Newfoundland and Labrador A1B 3V6, Canada.
authors.forename: Qi
authors.initials: Q
authors.lastname: Yuan
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020746:Calcium Channels, L-Type / Q000378:metabolism*; D004558:Electric Stimulation; D007545:Isoproterenol / Q000494:pharmacology; D051379:Mice; D009543:Nifedipine / Q000494:pharmacology; D009812:Odorants; D018408:Patch-Clamp Techniques; D066195:Piriform Cortex / Q000166:cytology*; D017966:Pyramidal Cells / Q000187:drug effects / Q000502:physiology*; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*
chemical_list: D000318:Adrenergic beta-Agonists; D002121:Calcium Channel Blockers; D020746:Calcium Channels, L-Type; D011943:Receptors, Adrenergic, beta; D009543:Nifedipine; D007545:Isoproterenol; D002118:Calcium