title: Editing of Mitochondrial Transcriptsnad3andcox2by Dek10 Is Essential for Mitochondrial Function and Maize Plant Development.
abstract: Respiration, the core of mitochondrial metabolism, depends on the function of five respiratory complexes. Many respiratory chain-related proteins are encoded by the mitochondrial genome and their RNAs undergo post-transcriptional modifications by nuclear genome-expressed factors, including pentatricopeptide repeat (PPR) proteins. Maizedefective kernel 10(dek10) is a classic mutant with small kernels and delayed development. Through positional cloning, we found thatDek10encodes an E-subgroup PPR protein localized in mitochondria. Sequencing analysis indicated that Dek10 is responsible for the C-to-U editing atnad3-61,nad3-62, andcox2-550 sites, which are specific editing sites in monocots. The defects of these editing sites result in significant reduction of Nad3 and the loss of Cox2. Interestingly, the assembly of complex I was not reduced, but its NADH dehydrogenase activity was greatly decreased. The assembly of complex IV was significantly reduced. Transcriptome and transmission electron microscopy (TEM) analysis revealed that proper editing ofnad3andcox2is critical for mitochondrial functions, biogenesis, and morphology. These results indicate that the E-subgroup PPR protein Dek10 is responsible for multiple editing sites innad3andcox2, which are essential for mitochondrial functions and plant development in maize.
authors.affiliation: Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, 200444, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Qi
authors.affiliation: Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, 200444, China.
authors.forename: Zhongrui
authors.initials: Z
authors.lastname: Tian
authors.affiliation: Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, 200444, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Lu
authors.affiliation: Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, 200444, China.
authors.forename: Xiuzu
authors.initials: X
authors.lastname: Chen
authors.affiliation: Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, 200444, China.
authors.forename: Xinze
authors.initials: X
authors.lastname: Chen
authors.affiliation: Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, 200444, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, 200444, China rentaosong@staff.shu.edu.cn.|National Maize Improvement Center of China, China Agricultural University, Beijing 100193, China.
authors.forename: Rentao
authors.initials: R
authors.lastname: Song
mesh_terms: D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D008928:Mitochondria / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D017393:RNA Editing; D012639:Seeds / Q000235:genetics / Q000378:metabolism; D003313:Zea mays / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D003576:Electron Transport Complex IV
keywords: RNA editing; Zea mays; dek10; mitochondrion; pentatricopeptide repeat protein; seed development