title: Multi-residue analysis of 36 priority and emerging pollutants in marine echinoderms (Holothuria tubulosa) and marine sediments by solid-liquid extraction followed by dispersive solid phase extraction and liquid chromatography-tandem mass spectrometry analysis.
abstract: Marine echinoderms are filter-feeding invertebrates widely distributed along the coasts, and which are therefore extensively exposed to anthropogenic xenobiotics. They can serve as good sentinels for monitoring a large variety of contaminants in marine ecosystems. In this context, a multi-residue analytical method has been validated and applied to Holothuria tubulosa specimens and marine sediments for the determination of 36 organic compounds, which belong to some of the most problematic groups of emerging and priority pollutants (perfluoroalkyl compounds, estrogens, parabens, benzophenones, plasticizers, surfactants, brominated flame retardants and alkylphenols). Lyophilization of samples prior to solvent extraction and clean-up of extracts with C18, followed by liquid chromatography-tandem mass spectrometry analysis, is proposed. A Box-Behnken design was used for optimization of the most influential variables affecting the extraction and clean-up steps. For validation, matrix-matched calibration and recovery assay were applied. Linearity (% r2) higher than 99%, recoveries between 80% and 114% (except in LAS and NP1EO), RSD (precision) lower than 15% and limits of quantification between 0.03 and 12.5ngg-1dry weight (d.w.) were achieved. The method was applied to nine samples of Holothuria collected along the coast of Granada (Spain), and to marine sediments around the animals. The results demonstrated high bioaccumulation of certain pollutants. A total of 25 out of the 36 studied compounds were quantified, being surfactants, alkylphenols, perfluoroalkyl compounds, triclocarban and parabens the most frequently detected. Nonylphenol was found in the highest concentration (340 and 323ngg-1d.w. in sediment and Holothuria samples, respectively).
authors.affiliation: Department of Analytical Chemistry, Superior Polytechnic School, University of Seville, C/ Virgen de África 7, E-41011 Seville, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Martín
authors.affiliation: Research Group of Analytical Chemistry and Life Sciences, Department of Analytical Chemistry, University of Granada, Campus of Fuentenueva, E-18071 Granada, Spain. Electronic address: azafra@ugr.es.
authors.forename: A
authors.initials: A
authors.lastname: Zafra-Gómez
authors.affiliation: Department of Zoology, University of Granada, Campus of Fuentenueva, E-18071 Granada, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Hidalgo
authors.affiliation: Agriculture and Fisheries Management Agency of Andalusia (AGAPA), Administrative Central Services, Av. of Greece s/n, 41012 Seville, Spain.
authors.forename: A J
authors.initials: AJ
authors.lastname: Ibáñez-Yuste
authors.affiliation: Department of Analytical Chemistry, Superior Polytechnic School, University of Seville, C/ Virgen de África 7, E-41011 Seville, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Alonso
authors.affiliation: Research Group of Analytical Chemistry and Life Sciences, Department of Analytical Chemistry, University of Granada, Campus of Fuentenueva, E-18071 Granada, Spain.
authors.forename: J L
authors.initials: JL
authors.lastname: Vilchez
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D019015:Geologic Sediments / Q000737:chemistry*; D047332:Holothuria / Q000737:chemistry*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D012997:Solvents / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004785:Environmental Pollutants; D012997:Solvents
keywords: Anthropogenic pollution; Holothuria tubulosa; LC–MS/MS; Priority and emerging pollutants; Sample preparation; Sediment