title: Candidate chemosensory genes identified in the endoparasitoid Meteorus pulchricornis (Hymenoptera: Braconidae) by antennal transcriptome analysis.
abstract: Meteorus pulchricornis is an endoparasitoid wasp which attacks the larvae of various lepidopteran pests. We present the first antennal transcriptome dataset for M. pulchricornis. A total of 48,845,072 clean reads were obtained and 34,967 unigenes were assembled. Of these, 15,458 unigenes showed a significant similarity (E-value <10-5) to known proteins in the NCBI non-redundant protein database. Gene ontology (GO) and cluster of orthologous groups (COG) analyses were used to classify the functions of M. pulchricornis antennae genes. We identified 16 putative odorant-binding protein (OBP) genes, eight chemosensory protein (CSP) genes, 99 olfactory receptor (OR) genes, 19 ionotropic receptor (IR) genes and one sensory neuron membrane protein (SNMP) gene. BLASTx best hit results and phylogenetic analysis both indicated that these chemosensory genes were most closely related to those found in other hymenopteran species. Real-time quantitative PCR assays showed that 14 MpulOBP genes were antennae-specific. Of these, MpulOBP6, MpulOBP9, MpulOBP10, MpulOBP12, MpulOBP15 and MpulOBP16 were found to have greater expression in the antennae than in other body parts, while MpulOBP2 and MpulOBP3 were expressed predominately in the legs and abdomens, respectively. These results might provide a foundation for future studies of olfactory genes and chemoreception in M. pulchricornis.
authors.affiliation: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, PR China. Electronic address: parasitoids@163.com.
authors.forename: Sheng
authors.initials: S
authors.lastname: Sheng
authors.affiliation: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China.
authors.forename: Cheng-Wu
authors.initials: CW
authors.lastname: Liao
authors.affiliation: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China.
authors.forename: Wen-Miao
authors.initials: WM
authors.lastname: Song
authors.affiliation: State Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: He
authors.affiliation: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, PR China. Electronic address: fuword@163.com.
authors.forename: Fu-An
authors.initials: FA
authors.lastname: Wu
mesh_terms: D000818:Animals; D057506:Arthropod Antennae / Q000254:growth & development / Q000378:metabolism*; D005260:Female; D019476:Insect Proteins / Q000235:genetics*; D007814:Larva / Q000235:genetics* / Q000254:growth & development; D060888:Real-Time Polymerase Chain Reaction; D018035:Receptors, Odorant / Q000235:genetics*; D059467:Transcriptome / Q000235:genetics*; D014863:Wasps / Q000235:genetics* / Q000254:growth & development
chemical_list: D019476:Insect Proteins; D018035:Receptors, Odorant; C045648:odorant-binding protein
keywords: Antennae; Chemosensory genes; Insect olfaction; Meteorus pulchricornis; Parasitoid wasp; Transcriptome