title: Identification and functional characterization of four novel aldo/keto reductases in Anabaena sp. PCC 7120 by integrating wet lab with in silico approaches.
abstract: Aldo/keto reductases (AKRs) constitute a multitasking protein family that catalyzes diverse metabolic transformations including detoxification of stress generated reactive aldehydes. Yet this important protein family is poorly understood particularly in cyanobacteria, the ecologically most diverse and significant group of micro-organisms. Present study is an attempt to characterize all putative AKRs of Anabaena sp. PCC 7120. In silico analysis, it revealed the presence of at least four putative AKRs in Anabaena PCC7120 genome. All four proteins share less than 40% sequence identity with each other and also with the identified members of AKR superfamily and hence deserve to be assigned in new families. Dissimilarity in sequences is also reflected through their substrate specificity. While reduction of trans-2-nonenal, a LPO-derived reactive aldehyde was common across the four proteins, these proteins were found to be activated during heat, salt, Cd, As, and butachlor treatments, and their ectopic expression in Escherichia coli conferred tolerance to the above abiotic stresses. These findings affirm the role of AKRs in providing a broad tolerance to environmental stresses conceivably by detoxifying the stress-generated reactive aldehydes.
authors.affiliation: Molecular Biology Section, Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi, 221005, India.
authors.forename: Chhavi
authors.initials: C
authors.lastname: Agrawal
authors.affiliation: Molecular Biology Section, Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi, 221005, India.
authors.forename: Shivam
authors.initials: S
authors.lastname: Yadav
authors.affiliation: Molecular Biology Section, Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi, 221005, India.
authors.forename: Shweta
authors.initials: S
authors.lastname: Rai
authors.affiliation: Molecular Biology Section, Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi, 221005, India.
authors.forename: Antra
authors.initials: A
authors.lastname: Chatterjee
authors.affiliation: Molecular Biology Section, Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi, 221005, India.
authors.forename: Sonia
authors.initials: S
authors.lastname: Sen
authors.affiliation: Molecular Biology Section, Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi, 221005, India.
authors.forename: Ruchi
authors.initials: R
authors.lastname: Rai
authors.affiliation: Molecular Biology Section, Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi, 221005, India. lcrbhu15@gmail.com.
authors.forename: L C
authors.initials: LC
authors.lastname: Rai
mesh_terms: D000074408:Aldo-Keto Reductases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017033:Anabaena / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017385:Sequence Homology; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D000074408:Aldo-Keto Reductases
keywords: Aldo/keto reductases; Anabaena PCC7120; Butachlor