title: Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED.
abstract: Traditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from pathologies that render them inappropriate for high-resolution structure determination. Here we show that fragmentation of large, imperfect crystals into microcrystals or nanocrystals can provide a simple path for high-resolution structure determination by the cryoEM method MicroED and potentially by serial femtosecond crystallography.
authors.affiliation: Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, Virginia, USA.
authors.forename: M Jason
authors.identifier: http://orcid.org/0000-0002-8573-5406
authors.initials: MJ
authors.lastname: de la Cruz
authors.affiliation: Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, Virginia, USA.
authors.forename: Johan
authors.identifier: http://orcid.org/0000-0002-8936-0912
authors.initials: J
authors.lastname: Hattne
authors.affiliation: Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, Virginia, USA.
authors.forename: Dan
authors.initials: D
authors.lastname: Shi
authors.affiliation: Howard Hughes Medical Institute, UCLA-DOE Institute, Departments of Biological Chemistry, Chemistry &Biochemistry, and Molecular Biology Institute, UCLA, Los Angeles, California, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Seidler
authors.affiliation: Howard Hughes Medical Institute, UCLA-DOE Institute, Departments of Biological Chemistry, Chemistry &Biochemistry, and Molecular Biology Institute, UCLA, Los Angeles, California, USA.
authors.forename: Jose
authors.initials: J
authors.lastname: Rodriguez
authors.affiliation: Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, Virginia, USA.
authors.forename: Francis E
authors.initials: FE
authors.lastname: Reyes
authors.affiliation: Howard Hughes Medical Institute, UCLA-DOE Institute, Departments of Biological Chemistry, Chemistry &Biochemistry, and Molecular Biology Institute, UCLA, Los Angeles, California, USA.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Sawaya
authors.affiliation: Howard Hughes Medical Institute, UCLA-DOE Institute, Departments of Biological Chemistry, Chemistry &Biochemistry, and Molecular Biology Institute, UCLA, Los Angeles, California, USA.
authors.forename: Duilio
authors.initials: D
authors.lastname: Cascio
authors.affiliation: Department of Structural Biology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
authors.forename: Simon C
authors.initials: SC
authors.lastname: Weiss
authors.affiliation: Department of Structural Biology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
authors.forename: Sun Kyung
authors.initials: SK
authors.lastname: Kim
authors.affiliation: Department of Structural Biology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
authors.forename: Cynthia S
authors.initials: CS
authors.lastname: Hinck
authors.affiliation: Department of Structural Biology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Hinck
authors.affiliation: Department of Structural Biology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Calero
authors.affiliation: Howard Hughes Medical Institute, UCLA-DOE Institute, Departments of Biological Chemistry, Chemistry &Biochemistry, and Molecular Biology Institute, UCLA, Los Angeles, California, USA.
authors.forename: David
authors.initials: D
authors.lastname: Eisenberg
authors.affiliation: Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, Virginia, USA.
authors.forename: Tamir
authors.initials: T
authors.lastname: Gonen
mesh_terms: D020285:Cryoelectron Microscopy / Q000379:methods*; D003461:Crystallography / Q000379:methods*; D018360:Crystallography, X-Ray / Q000379:methods; D008958:Models, Molecular; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins