title: Proteomic responses of European flounder to temperature and hypoxia as interacting stressors: Differential sensitivities of populations.
abstract: In the context of global change, ectotherms are increasingly impacted by abiotic perturbations. Along the distribution area of a species, the populations at low latitudes are particularly exposed to temperature increase and hypoxic events. In this study, we have compared the proteomic responses in the liver of European flounder populations, by using 2-D electrophoresis. One southern peripheral population from Portugal vs two northern core populations from France, were reared in a common garden experiment. Most of the proteomic differences were observed between the two experimental conditions, a cold vs a warm and hypoxic conditions. Consistent differentiations between populations were observed in accumulation of proteins involved in the bioenergetics- and methionine-metabolisms, fatty acids transport, and amino-acid catabolism. The specific regulation of crucial enzymes like ATP-synthase and G6PDH, in the liver of the southern population, could be related to a possible local adaptation. This southern peripheral population is spatially distant from northern core populations and has experienced dissimilar ecological conditions; thus it may contain genotypes that confer resilience to climate changes.
authors.affiliation: Université de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/Ifremer, Laboratoire des Sciences de l'Environnement Marin LEMAR, Institut Universitaire Européen de la Mer IUEM, Plouzané, France; Ifremer, Laboratoire Adaptation, Reproduction et Nutrition des Poissons ARN, Unité de Physiologie Fonctionnelle des Organismes Marins PFOM, Plouzané, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Pédron
authors.affiliation: Université de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/Ifremer, Laboratoire des Sciences de l'Environnement Marin LEMAR, Institut Universitaire Européen de la Mer IUEM, Plouzané, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Artigaud
authors.affiliation: Université de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/Ifremer, Laboratoire des Sciences de l'Environnement Marin LEMAR, Institut Universitaire Européen de la Mer IUEM, Plouzané, France; Ifremer, Laboratoire Adaptation, Reproduction et Nutrition des Poissons ARN, Unité de Physiologie Fonctionnelle des Organismes Marins PFOM, Plouzané, France.
authors.forename: José-Luis Zambonino
authors.initials: JZ
authors.lastname: Infante
authors.affiliation: Université de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/Ifremer, Laboratoire des Sciences de l'Environnement Marin LEMAR, Institut Universitaire Européen de la Mer IUEM, Plouzané, France; Ifremer, Laboratoire Adaptation, Reproduction et Nutrition des Poissons ARN, Unité de Physiologie Fonctionnelle des Organismes Marins PFOM, Plouzané, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Le Bayon
authors.affiliation: Université de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/Ifremer, Laboratoire des Sciences de l'Environnement Marin LEMAR, Institut Universitaire Européen de la Mer IUEM, Plouzané, France.
authors.forename: Grégory
authors.initials: G
authors.lastname: Charrier
authors.affiliation: Université de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/Ifremer, Laboratoire des Sciences de l'Environnement Marin LEMAR, Institut Universitaire Européen de la Mer IUEM, Plouzané, France.
authors.forename: Vianney
authors.initials: V
authors.lastname: Pichereau
authors.affiliation: Université de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/Ifremer, Laboratoire des Sciences de l'Environnement Marin LEMAR, Institut Universitaire Européen de la Mer IUEM, Plouzané, France. Electronic address: jean.laroche@univ-brest.fr.
authors.forename: Jean
authors.initials: J
authors.lastname: Laroche
mesh_terms: D000818:Animals; D057231:Climate Change; D005432:Flounder / Q000378:metabolism*; D005602:France; D000860:Hypoxia; D011174:Portugal; D020543:Proteome; D013312:Stress, Physiological; D013696:Temperature
chemical_list: D020543:Proteome
keywords: Fish populations; Hypoxia; Multiple stressors; Proteomics; Temperature rising