title: Activity of inflammatory bowel disease influences the expression of cytokines in gingival tissue.
abstract: This study assessed the cytokine expression in gingival and intestinal tissues from periodontitis patients with inflammatory bowel disease (IBD) and evaluated if IBD activity is a covariate to the amount of gingival cytokines. Paired gingival and intestinal tissues were collected from 21 patients and homogenised using a cell disruptor. Cytokine expression (IL-1β, IL-4, IL-6, IL-10, IL-21, IL-22, IL-23, IL-25, IL-31, IL-33, IL-17A, IL-17F, IFN-γ, sCD40L, and TNF-α) was evaluated using bead-based multiplex technology. An inflammation score was developed using the intestinal cytokines that showed good accuracy to discriminate IBD active patients from those in remission and then a similar score was applied to gingival tissue. IL-4, IL-10 and IL-21 expressions were significantly increased in gingival tissue from patients with an active disease as compared to those with a disease in remission. The inflammation score (mean value of IL-1β, IL-6, IL-21, and sCD40L) was significantly higher in gingival tissue from patients with IBD activity. There was a significant correlation between gingival and intestinal inflammation scores (rho=0.548; P=0.01). Significantly higher IL-23 and IFN-γ levels and lower IL-31 and TNF-α levels were observed in gingival tissues than in intestinal ones. Activity of inflammatory bowel disease influenced the cytokine expression in gingival tissue.
authors.affiliation: Department of Periodontology, Faculty of Odontology, Rio de Janeiro State University, Rio de Janeiro, Brazil. Electronic address: cmfigueredo@hotmail.com.
authors.forename: C M
authors.initials: CM
authors.lastname: Figueredo
authors.affiliation: Department of Periodontology, Faculty of Odontology, Rio de Janeiro State University, Rio de Janeiro, Brazil.
authors.forename: A P
authors.initials: AP
authors.lastname: Martins
authors.affiliation: Department of Periodontology, Faculty of Odontology, Rio de Janeiro State University, Rio de Janeiro, Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Lira-Junior
authors.affiliation: Department of Periodontology, Faculty of Odontology, Rio de Janeiro State University, Rio de Janeiro, Brazil.
authors.forename: J B
authors.initials: JB
authors.lastname: Menegat
authors.affiliation: Department of Internal Medicine, Faculty of Medicine, Rio de Janeiro State University, Rio de Janeiro, Brazil.
authors.forename: A T
authors.initials: AT
authors.lastname: Carvalho
authors.affiliation: Department of Periodontology, Faculty of Odontology, Rio de Janeiro State University, Rio de Janeiro, Brazil.
authors.forename: R G
authors.initials: RG
authors.lastname: Fischer
authors.affiliation: Division of Periodontology, Department of Dental Medicine, Karolinska Institutet, Stockholm, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Gustafsson
mesh_terms: D000328:Adult; D003430:Cross-Sectional Studies; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D005881:Gingiva / Q000276:immunology*; D006801:Humans; D007249:Inflammation / Q000276:immunology; D015212:Inflammatory Bowel Diseases / Q000188:drug therapy / Q000276:immunology*; D007422:Intestines / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000276:immunology*; D012074:Remission Induction; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors; D055815:Young Adult
chemical_list: D016207:Cytokines; D014409:Tumor Necrosis Factor-alpha
keywords: Cytokines; Inflammatory bowel disease; Periodontitis