title: Long non-coding RNA PTENP1 functions as a ceRNA to modulate PTEN level by decoying miR-106b and miR-93 in gastric cancer.
abstract: Recent studies have shown that competing endogenous RNAs (ceRNAs) play an important role in the regulation of gene expression, and participate in a wide range of biological processes, including carcinogenesis. Long non-coding RNA PTENP1, the pseudogene of PTEN tumor suppressor, has been reported to exert its tumor suppressive function via modulation of PTEN expression in many malignancies. However, whether a PTENP1~miRNA~PTEN ceRNA network exists and how it functions in gastric cancer (GC) remains elusive. In order to identify and characterize the PTENP1~miRNA~PTEN ceRNA network in GC, we first determined PTENP1 levels in clinical GC samples and found that PTENP1 and PTEN were concurrently downregulated in these samples. We further demonstrated that PTENP1 could act as a ceRNA to sponge miR-106b and miR-93 from targeting PTEN for downregulation using a novel ceRNA in vitro gradient assay. Thus, we revealed a tumor suppressive role of PTENP1 as ceRNA in GC and pinpointed the specific miRNAs decoyed by PTENP1, highlighting the emerging roles of ceRNAs in the biological regulation of GC cells and their possible clinical significance.
authors.affiliation: Department of Gastric Cancer Surgery, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China.|National Clinical Research Center of Cancer, Tianjin 300060, China.|Tianjin's Clinical Research Center for Cancer, Tianjin 300060, China.
authors.forename: Rupeng
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Gastric Cancer Surgery, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China.|National Clinical Research Center of Cancer, Tianjin 300060, China.|Tianjin's Clinical Research Center for Cancer, Tianjin 300060, China.
authors.forename: Yuenan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Gastric Cancer Surgery, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China.|National Clinical Research Center of Cancer, Tianjin 300060, China.|Tianjin's Clinical Research Center for Cancer, Tianjin 300060, China.
authors.forename: Zhenchi
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Department of Gastrointestinal Cancer Biology, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China.|National Clinical Research Center of Cancer, Tianjin 300060, China.|Tianjin's Clinical Research Center for Cancer, Tianjin 300060, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Ma
authors.affiliation: Department of Gastric Cancer Surgery, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China.|National Clinical Research Center of Cancer, Tianjin 300060, China.|Tianjin's Clinical Research Center for Cancer, Tianjin 300060, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xue
authors.affiliation: Department of Cancer Prevention Center, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China.|National Clinical Research Center of Cancer, Tianjin 300060, China.|Tianjin's Clinical Research Center for Cancer, Tianjin 300060, China.
authors.forename: Fangxuan
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Gastrointestinal Cancer Biology, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China.|National Clinical Research Center of Cancer, Tianjin 300060, China.|Tianjin's Clinical Research Center for Cancer, Tianjin 300060, China.
authors.forename: Liren
authors.initials: L
authors.lastname: Liu
mesh_terms: D020413:3' Untranslated Regions; D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D060787:Neoplasm Grading; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D051059:PTEN Phosphohydrolase / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D047368:Tumor Burden
chemical_list: D020413:3' Untranslated Regions; C542541:MIRN106 microRNA, human; C543350:MIRN93 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human
keywords: PTENP1; gastric cancer; long non-coding RNA; miR-106b; miR-93