title: The Arp2/3 inhibitory protein Arpin is dispensable for chemotaxis.
abstract: Arpin is an Arp2/3 inhibitory protein, which decreases the protrusion lifetime and hence directional persistence in the migration of diverse cells. Arpin is activated by the small GTPase Rac, which controls cell protrusion, thus closing a negative feedback loop that renders the protrusion intrinsically unstable. Because of these properties, it was proposed that Arpin might play a role in directed migration, where directional persistence has to be fine-tuned. We report here, however, that Arpin-depleted tumour cells and Arpin knock-out Dictyostelium amoeba display no obvious defect in chemotaxis. These results do not rule out a potential role of Arpin in other systems, but argue against a general role of Arpin in chemotaxis.
authors.affiliation: Ecole Polytechnique, Université Paris-Saclay, CNRS UMR7654, Palaiseau, 91120, France.
authors.forename: Irène
authors.initials: I
authors.lastname: Dang
authors.affiliation: Institute for Biophysical Chemistry, Hannover Medical School, Hannover, 30623, Germany.
authors.forename: Joern
authors.initials: J
authors.lastname: Linkner
authors.affiliation: Massachusetts General Hospital and Harvard Medical School, BioMEMS Resource Center, Boston, MA, 02129, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Yan
authors.affiliation: Massachusetts General Hospital and Harvard Medical School, BioMEMS Resource Center, Boston, MA, 02129, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Irimia
authors.affiliation: Institute for Biophysical Chemistry, Hannover Medical School, Hannover, 30623, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Faix
authors.affiliation: Ecole Polytechnique, Université Paris-Saclay, CNRS UMR7654, Palaiseau, 91120, France.
authors.forename: Alexis
authors.initials: A
authors.lastname: Gautreau
mesh_terms: D051377:Actin-Related Protein 2 / Q000235:genetics / Q000378:metabolism; D051378:Actin-Related Protein 3 / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002633:Chemotaxis / Q000502:physiology*; D004023:Dictyostelium / Q000378:metabolism; D006801:Humans
chemical_list: D051377:Actin-Related Protein 2; D051378:Actin-Related Protein 3; D002352:Carrier Proteins
keywords: Actin; Cell migration; Cytoskeleton