title: Genotoxicity Evaluation of an Urban River on Freshwater Planarian by RAPD Assay.
abstract: The aim of this study was to evaluate the genotoxic potential of an urban river - the Wei River in Xinxiang, China using randomly amplified polymorphic DNA (RAPD) assay in planarians. The results showed that the total number of polymorphic bands and varied bands in RAPD patterns of treated planarians decreased with the water sample site far away from the sewage outlet of a factory. In addition, the genome template stability of treated groups decreased and the degree of the decline was negatively related to the distance between the sample site and the sewage outlet, suggesting that the Wei River water had genotoxicity effects on planarians and strengthening the management of the Wei River was necessary. Furthermore, this work also indicated that RAPD assay in planarians was a very promising test for environmental monitoring studies.
authors.affiliation: College of Life Sciences, Henan Normal University, No. 46, Jianshe East Road, Xinxiang, 453007, China.
authors.forename: He-Cai
authors.initials: HC
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Henan Normal University, No. 46, Jianshe East Road, Xinxiang, 453007, China.
authors.forename: Tong-Yi
authors.initials: TY
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Henan Normal University, No. 46, Jianshe East Road, Xinxiang, 453007, China.
authors.forename: Chang-Ying
authors.initials: CY
authors.lastname: Shi
authors.affiliation: College of Life Sciences, Henan Normal University, No. 46, Jianshe East Road, Xinxiang, 453007, China. chengw0183@sina.com.
authors.forename: Guang-Wen
authors.initials: GW
authors.lastname: Chen
authors.affiliation: Institute of Natural Resources, Heilongjiang Academy of Science, Harbin, 150031, China.
authors.forename: De-Zeng
authors.initials: DZ
authors.lastname: Liu
mesh_terms: D000818:Animals; D002681:China; D004249:DNA Damage; D004784:Environmental Monitoring / Q000379:methods; D010932:Planarians / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D045483:Rivers / Q000737:chemistry*; D014876:Water Pollution / Q000009:adverse effects*
keywords: Freshwater planarian; Genotoxicity; RAPD; Urban river