title: Structural elucidation and antioxidant activity a novel Se-polysaccharide from Se-enriched Grifola frondosa.
abstract: Se-GFP-22, a heteropolysaccharide, with a weight-average Mwof 4.13×106Da, was purified from the crude Se-polysaccharide (Se-GFP) isolated from fruit bodies of Se-enriched Grifola frondosa. Selenium was accumulated efficiently in Grifola frondosa during cultivation with Na2SeO3. The structure was investigated through FT-IR, GC, GC-MS, NMR, HPSEC-MALL-RI, particle size, Conge-red test, CD, AFM and SEM. Se-GFP-22 was deduced as a backbone chain of 1,4-α-d-Glcp units with a branched point at C6 of both 1,3,6-β-d-Manp and 1,4,6-α-d-Galp units. A typical absorption for selenium ester was existed in Se-GFP-22. Se-GFP-22 adopted as a spherical conformation with random coils. A novel Se-polysaccharide of different monosaccharide constituents, molecular weight, linkage types and high content of selenium has been isolated from G. frondosa. The antioxidant effect of Se-GFP-22 was more potent than that of G. frondosa polysaccharide (GFP-22), which may be influenced by the co-effect of polysaccharide and Se, molecular weight, degree of branching and configuration.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: School of the Environment, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, Jiangsu, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China.
authors.forename: Weijie
authors.initials: W
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Yu
authors.affiliation: School of the Environment, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, Jiangsu, China.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Mao
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhao
authors.affiliation: School of the Environment, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, Jiangsu, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Feng
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China. Electronic address: yangliuqing@ujs.edu.cn.
authors.forename: Liuqing
authors.initials: L
authors.lastname: Yang
authors.affiliation: School of the Environment, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, Jiangsu, China. Electronic address: wuxy@ujs.edu.cn.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Wu
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D048691:Grifola / Q000737:chemistry*; D008968:Molecular Conformation; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D012643:Selenium / Q000737:chemistry* / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000975:Antioxidants; D011134:Polysaccharides; D012643:Selenium
keywords: Antioxidant activity; Chain conformation; Polysaccharide; Se-enriched Grifola frondosa; Structural elucidation; d-Galactose (PubChem CID: 6036); d-Glucose (PubChem CID: 5793); d-Mannose (PubChem CID: 18950)