title: Probing the molecular determinants of fluorinase specificity.
abstract: Molecular determinants of FlA1 fluorinase specificity were probed using 5'-chloro-5'-deoxyadenosine (5'-ClDA) analogs as substrates and FlA1 active site mutants. Modifications at F213 or A279 residues are beneficial towards these modified substrates, including 5'-chloro-5'-deoxy-2-ethynyladenosine, ClDEA (>10-fold activity improvement), and conferred novel activity towards substrates not readily accepted by wild-type FlA1.
authors.affiliation: Metabolic Engineering Research Laboratory (MERL), Science and Engineering Institutes, Agency for Science, Technology and Research (A*STAR), 31 Biopolis Way, Nanos #01-01, Singapore 138669. angel@merl.a-star.edu.sg.
authors.forename: W L
authors.initials: WL
authors.lastname: Yeo
authors.affiliation: Institute of Chemical and Engineering Sciences (ICES), A*STAR, 8 Biomedical Grove, Neuros #07-01/02/03, Singapore 138665. lim_yee_hwee@ices.a-star.edu.sg.
authors.forename: X
authors.initials: X
authors.lastname: Chew
authors.affiliation: Bioinformatics Institute, A*STAR, 30 Biopolis Street, Matrix #07-01, Singapore 138671 and Biotransformation Innovation Platform, A*STAR, 61 Biopolis Drive, Proteos #04-14, Singapore 138673.
authors.forename: D J
authors.initials: DJ
authors.lastname: Smith
authors.affiliation: Institute of Chemical and Engineering Sciences (ICES), A*STAR, 8 Biomedical Grove, Neuros #07-01/02/03, Singapore 138665. lim_yee_hwee@ices.a-star.edu.sg.
authors.forename: K P
authors.initials: KP
authors.lastname: Chan
authors.affiliation: Metabolic Engineering Research Laboratory (MERL), Science and Engineering Institutes, Agency for Science, Technology and Research (A*STAR), 31 Biopolis Way, Nanos #01-01, Singapore 138669. angel@merl.a-star.edu.sg.
authors.forename: H
authors.initials: H
authors.lastname: Sun
authors.affiliation: Metabolic Engineering Research Laboratory (MERL), Science and Engineering Institutes, Agency for Science, Technology and Research (A*STAR), 31 Biopolis Way, Nanos #01-01, Singapore 138669. angel@merl.a-star.edu.sg and 215 Roger Adams Laboratory, Box C3, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue Urbana, IL 61801, USA.
authors.forename: H
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Institute of Chemical and Engineering Sciences (ICES), A*STAR, 8 Biomedical Grove, Neuros #07-01/02/03, Singapore 138665. lim_yee_hwee@ices.a-star.edu.sg.
authors.forename: Y H
authors.initials: YH
authors.lastname: Lim
authors.affiliation: Metabolic Engineering Research Laboratory (MERL), Science and Engineering Institutes, Agency for Science, Technology and Research (A*STAR), 31 Biopolis Way, Nanos #01-01, Singapore 138669. angel@merl.a-star.edu.sg.
authors.forename: E L
authors.identifier: http://orcid.org/0000-0002-3070-8056
authors.initials: EL
authors.lastname: Ang
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D017338:Cladribine / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D015335:Molecular Probes / Q000737:chemistry / Q000378:metabolism*; D009154:Mutation; D009690:Nucleic Acid Conformation; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D015335:Molecular Probes; D017338:Cladribine; C044739:5'-chloro-5'-deoxyadenosine; D010088:Oxidoreductases; C495766:fluorinase