title: Biosensing of DNA oxidative damage: a model of using glucose meter for non-glucose biomarker detection.
abstract: Non-glucose biomarker-DNA oxidative damage biomarker 8-hydroxy-2'-deoxyguanosine (8-OHdG) has been successfully detected using a smartphone-enabled glucose meter. Through a series of immune reactions and enzymatic reactions on a solid lateral flow platform, 8-OHdG concentration has been converted to a relative amount of glucose, and therefore can be detected by conventional glucose meter directly. The device was able to detect 8-OHdG concentrations in phosphate buffer saline as low as 1.73 ng mL-1with a dynamic range of 1-200 ng mL-1. Considering the inherent advantages of the personal glucose meter, the demonstration of this device, therefore, should provide new opportunities for the monitoring of a wide range of biomarkers and various target analytes in connection with different molecular recognition events.
authors.affiliation: Nanobioengineering/Bioelectronics Laboratory, Department of Biomedical Engineering, Florida International University, Miami, FL, USA.
authors.forename: Xuena
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Nanobioengineering/Bioelectronics Laboratory, Department of Biomedical Engineering, Florida International University, Miami, FL, USA.
authors.forename: Mehenur
authors.initials: M
authors.lastname: Sarwar
authors.affiliation: Department of Chemistry, College of Chemistry and Chemical Engineering, Liao Chen University, Shandong.
authors.forename: Qiaoli
authors.initials: Q
authors.lastname: Yue
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing.
authors.forename: Chunying
authors.initials: C
authors.lastname: Chen
authors.affiliation: Nanobioengineering/Bioelectronics Laboratory, Department of Biomedical Engineering, Florida International University, Miami, FL, USA; Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, People's Republic of China.
authors.forename: Chen-Zhong
authors.initials: CZ
authors.lastname: Li
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D015190:Blood Glucose Self-Monitoring / Q000295:instrumentation*; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis; D004867:Equipment Design; D005947:Glucose / Q000378:metabolism; D006151:Guanosine / Q000031:analogs & derivatives / Q000737:chemistry; D010084:Oxidation-Reduction; D000068997:Smartphone; D043324:beta-Fructofuranosidase / Q000737:chemistry / Q000378:metabolism
chemical_list: D015415:Biomarkers; D006151:Guanosine; C046215:8-hydroxyguanosine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D043324:beta-Fructofuranosidase; D003849:Deoxyguanosine; D005947:Glucose
keywords: 8-OHdG; DNA oxidative damage; POCTs; biosensor; immunostrip; point-of-care