title: Multitarget sensing of glucose and cholesterol based on Janus hydrogel microparticles.
abstract: A visualized sensing method for glucose and cholesterol was developed based on the hemispheres of the same Janus hydrogel microparticles. Single-phase and Janus hydrogel microparticles were both generated using a centrifugal microfluidic chip. For glucose sensing, concanavalin A and fluorescein labeled dextran used for competitive binding assay were encapsulated in alginate microparticles, and the fluorescence of the microparticles was positively correlated with glucose concentration. For cholesterol sensing, the microparticles embedded with γ-Fe2O3nanoparticles were used as catalyst for the oxidation of 3,3',5,5'-Tetramethylbenzidine by H2O2, an enzymatic hydrolysis product of cholesterol. And the color transition was more sensitive in the microparticles than in solutions, indicating the microparticles are more applicable for visualized determination. Furthermore, Janus microparticles were employed for multitarget sensing in the two hemespheres, and glucose and cholesterol were detected within the same microparticles without obvious interference. Besides, the particles could be manipulated by an external magnetic field. The glucose and cholesterol levels were measured in human serum utilizing the microparticles, which confirmed the potential application of the microparticles in real sample detection.
authors.affiliation: Research Center for Analytical Sciences, Northeastern University, Shenyang 110819, PR China.
authors.forename: Xiao-Ting
authors.initials: XT
authors.lastname: Sun
authors.affiliation: Research Center for Analytical Sciences, Northeastern University, Shenyang 110819, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Research Center for Analytical Sciences, Northeastern University, Shenyang 110819, PR China.
authors.forename: Dong-Hua
authors.initials: DH
authors.lastname: Zheng
authors.affiliation: Research Center for Analytical Sciences, Northeastern University, Shenyang 110819, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Yue
authors.affiliation: Research Center for Analytical Sciences, Northeastern University, Shenyang 110819, PR China.
authors.forename: Chun-Guang
authors.initials: CG
authors.lastname: Yang
authors.affiliation: Research Center for Analytical Sciences, Northeastern University, Shenyang 110819, PR China. Electronic address: xuzr@mail.neu.edu.cn.
authors.forename: Zhang-Run
authors.initials: ZR
authors.lastname: Xu
mesh_terms: D000464:Alginates / Q000737:chemistry*; D001560:Benzidines / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis*; D002784:Cholesterol / Q000097:blood*; D003208:Concanavalin A / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D056656:Lab-On-A-Chip Devices; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction
chemical_list: D000464:Alginates; D001560:Benzidines; D001786:Blood Glucose; D003911:Dextrans; D005290:Ferric Compounds; D005456:Fluorescent Dyes; D006603:Hexuronic Acids; D003208:Concanavalin A; C000499:ferric oxide; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C021758:3,3',5,5'-tetramethylbenzidine; D020723:Glucuronic Acid; D002784:Cholesterol; D006861:Hydrogen Peroxide
keywords: Centrifugal microfluidic chip; Cholesterol; Glucose; Janus microparticles; Multitarget sensing