title: Changes in protein expression after treatment with Ancylostoma caninum excretory/secretory products in a mouse model of colitis.
abstract: Different reports have highlighted the potential use of helminths and their secretions in the treatment of inflammatory bowel disease (IBD) conditions; however, no reports have investigated their effects at a proteome level. Herein, we characterise the protein expression changes that occur in lamina propria (LP) and the intestinal epithelial cells (IEC) of mice with dextran sulfate sodium (DSS)-induced colitis treated with Ancylostoma caninum excretory/secretory (ES) products using a quantitative proteomic approach. We have shown how parasite products can significantly alter the expression of proteins involved in immune responses, cell death and with an antioxidant activity. Interestingly, significant changes in the expression levels of different mucins were observed in this study. MUC13, a mucin implicated in gastrointestinal homeostasis, was upregulated in the LP of mice with DSS-induced colitis treated with ES, while MUC2, a major component of mucus, was upregulated in the IEC. In addition, A. caninum proteins have an important effect on proteins with antioxidant functions and proteins involved in intestinal homeostasis and tissue integrity and regeneration. Understanding how parasites can ameliorate IBD pathogenesis can help us design novel treatments for autoimmune diseases.
authors.affiliation: Centre for Biodiscovery and Molecular Development of Therapeutics, Australian Institute of Tropical Health and Medicine, James Cook University, Cairns, QLD, Australia.
authors.forename: Javier
authors.initials: J
authors.lastname: Sotillo
authors.affiliation: Centre for Biodiscovery and Molecular Development of Therapeutics, Australian Institute of Tropical Health and Medicine, James Cook University, Cairns, QLD, Australia.
authors.forename: Ivana
authors.initials: I
authors.lastname: Ferreira
authors.affiliation: QIMR Berghofer Medical Research Institute, Brisbane, QLD, Australia.
authors.forename: Jeremy
authors.initials: J
authors.lastname: Potriquet
authors.affiliation: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Thewarach
authors.initials: T
authors.lastname: Laha
authors.affiliation: Centre for Biodiscovery and Molecular Development of Therapeutics, Australian Institute of Tropical Health and Medicine, James Cook University, Cairns, QLD, Australia.
authors.forename: Severine
authors.initials: S
authors.lastname: Navarro
authors.affiliation: Centre for Biodiscovery and Molecular Development of Therapeutics, Australian Institute of Tropical Health and Medicine, James Cook University, Cairns, QLD, Australia.
authors.forename: Alex
authors.initials: A
authors.lastname: Loukas
authors.affiliation: QIMR Berghofer Medical Research Institute, Brisbane, QLD, Australia.|The University of Queensland, School of Biomedical Sciences, Brisbane 4072, Australia.
authors.forename: Jason
authors.initials: J
authors.lastname: Mulvenna
mesh_terms: D000722:Ancylostoma / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism; D001688:Biological Products / Q000494:pharmacology* / Q000627:therapeutic use; D003093:Colitis, Ulcerative / Q000188:drug therapy* / Q000209:etiology; D016264:Dextran Sulfate / Q000633:toxicity; D004815:Epidermal Growth Factor / Q000235:genetics / Q000378:metabolism; D005260:Female; D015801:Helminth Proteins / Q000494:pharmacology* / Q000627:therapeutic use; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D055262:Mucin-2 / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D000893:Anti-Inflammatory Agents; D000954:Antigens, Surface; D001688:Biological Products; D015801:Helminth Proteins; C063487:Ly64 protein, mouse; C524626:Muc2 protein, mouse; D055262:Mucin-2; D004815:Epidermal Growth Factor; D016264:Dextran Sulfate