title: Eosinophils Regulate Interferon Alpha Production in Plasmacytoid Dendritic Cells Stimulated with Components of Neutrophil Extracellular Traps.
abstract: Eosinophils constitute an important component of helminth immunity and are not only associated with various allergies but are also linked to autoinflammatory disorders, including the skin disease psoriasis. Here we demonstrate the functional relationship between eosinophils and plasmacytoid dendritic cells (pDCs) as related to skin diseases. We previously showed that pDCs colocalize with neutrophil extracellular traps (NETs) in psoriatic skin. Here we demonstrate that eosinophils are found in psoriatic skin near neutrophils and NETs, suggesting that pDC responses can be regulated by eosinophils. Eosinophils inhibited pDC function in vitro through a mechanism that did not involve cell contact but depended on soluble factors. In pDCs stimulated by specific NET components, eosinophil-conditioned media attenuated the production of interferon α (IFNα) but did not affect the maturation of pDCs as evidenced by the unaltered expression of the costimulatory molecules CD80 and CD86. As pDCs and IFNα play a key role in autoimmune skin inflammation, these data suggest that eosinophils may influence autoinflammatory responses through their impact on the production of IFNα by pDCs.
authors.affiliation: 1 Faculty of Biochemistry, Biophysics and Biotechnology, Department of Immunology, Jagiellonian University , Krakow, Poland .
authors.forename: Joanna
authors.initials: J
authors.lastname: Skrzeczynska-Moncznik
authors.affiliation: 1 Faculty of Biochemistry, Biophysics and Biotechnology, Department of Immunology, Jagiellonian University , Krakow, Poland .
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Zabieglo
authors.affiliation: 1 Faculty of Biochemistry, Biophysics and Biotechnology, Department of Immunology, Jagiellonian University , Krakow, Poland .
authors.forename: Jozef P
authors.initials: JP
authors.lastname: Bossowski
authors.affiliation: 1 Faculty of Biochemistry, Biophysics and Biotechnology, Department of Immunology, Jagiellonian University , Krakow, Poland .
authors.forename: Oktawia
authors.initials: O
authors.lastname: Osiecka
authors.affiliation: 1 Faculty of Biochemistry, Biophysics and Biotechnology, Department of Immunology, Jagiellonian University , Krakow, Poland .
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Wlodarczyk
authors.affiliation: 2 Department of Dermatology, Zeromski Hospital , Krakow, Poland .
authors.forename: Monika
authors.initials: M
authors.lastname: Kapinska-Mrowiecka
authors.affiliation: 1 Faculty of Biochemistry, Biophysics and Biotechnology, Department of Immunology, Jagiellonian University , Krakow, Poland .
authors.forename: Mateusz
authors.initials: M
authors.lastname: Kwitniewski
authors.affiliation: 1 Faculty of Biochemistry, Biophysics and Biotechnology, Department of Immunology, Jagiellonian University , Krakow, Poland .
authors.forename: Pawel
authors.initials: P
authors.lastname: Majewski
authors.affiliation: 3 Faculty of Biochemistry, Biophysics and Biotechnology, Department of Analytical Biochemistry, Jagiellonian University , Krakow, Poland .
authors.forename: Adam
authors.initials: A
authors.lastname: Dubin
authors.affiliation: 1 Faculty of Biochemistry, Biophysics and Biotechnology, Department of Immunology, Jagiellonian University , Krakow, Poland .
authors.forename: Joanna
authors.initials: J
authors.lastname: Cichy
mesh_terms: D000328:Adult; D015550:Cell Degranulation / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism*; D004804:Eosinophils / Q000276:immunology* / Q000378:metabolism*; D065206:Extracellular Traps / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D016898:Interferon-alpha / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism*; D011565:Psoriasis / Q000175:diagnosis / Q000276:immunology / Q000378:metabolism; D012720:Severity of Illness Index; D055815:Young Adult
chemical_list: D016898:Interferon-alpha
keywords: dendritic cell; granulocyte; neutrophil elastase; psoriasis; secretory leukocyte protease inhibitor