title: Beech wood Fagus sylvatica dilute-acid hydrolysate as a feedstock to support Chlorella sorokiniana biomass, fatty acid and pigment production.
abstract: This work evaluates the possibility of using beech wood (Fagus sylvatica) dilute-acid (H2SO4) hydrolysate as a feedstock for Chlorella sorokiniana growth, fatty acid and pigment production. Neutralized wood acid hydrolysate, containing organic and mineral compounds, was tested on Chlorella growth at different concentrations and compared to growth under phototrophic conditions. Chlorella growth was improved at lower loadings and inhibited at higher loadings. Based on these results, a 12% neutralized wood acid hydrolysate (Hyd12%) loading was selected to investigate its impact on Chlorella growth, fatty acid and pigment production. Hyd12% improved microalgal biomass, fatty acid and pigment productivities both in light and in dark, when compared to photoautotrophic control. Light intensity had substantial influence on fatty acid and pigment composition in Chlorella culture during Hyd12%-based growth. Moreover, heterotrophic Chlorella cultivation with Hyd12% also showed that wood hydrolysate can constitute an attractive feedstock for microalgae cultivation in case of lack of light.
authors.affiliation: TERRA, AgricultureIsLife Platform, University of Liege-Gembloux Agro-Bio Tech, Passage des Déportés 2, Gembloux B-5030, Belgium; Unit of Biological and Industrial Chemistry, University of Liege-Gembloux Agro-Bio Tech, Passage des Déportés 2, Gembloux B-5030, Belgium. Electronic address: kmiazek@ulg.ac.be.
authors.forename: Krystian
authors.initials: K
authors.lastname: Miazek
authors.affiliation: Genetics and Physiology of Microalgae, Institute of Botany, University of Liege, B22, Chemin de la vallée, Liège B-4000, Belgium.
authors.forename: Claire
authors.initials: C
authors.lastname: Remacle
authors.affiliation: Unit of Biological and Industrial Chemistry, University of Liege-Gembloux Agro-Bio Tech, Passage des Déportés 2, Gembloux B-5030, Belgium.
authors.forename: Aurore
authors.initials: A
authors.lastname: Richel
authors.affiliation: Cellule Innovation et Créativité, University of Liege-Gembloux Agro-Bio Tech, Passage des Déportés 2, Gembloux B-5030, Belgium.
authors.forename: Dorothee
authors.initials: D
authors.lastname: Goffin
mesh_terms: D056804:Biofuels; D018533:Biomass; D002244:Carbon / Q000494:pharmacology; D002708:Chlorella / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D029964:Fagus / Q000737:chemistry* / Q000187:drug effects; D005227:Fatty Acids / Q000096:biosynthesis*; D052836:Heterotrophic Processes / Q000187:drug effects; D006868:Hydrolysis; D058086:Microalgae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D009930:Organic Chemicals / Q000494:pharmacology; D010860:Pigments, Biological / Q000096:biosynthesis*; D013464:Sulfuric Acids / Q000494:pharmacology*; D014934:Wood / Q000737:chemistry* / Q000187:drug effects
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D009930:Organic Chemicals; D010860:Pigments, Biological; D013464:Sulfuric Acids; D002244:Carbon; C033158:sulfuric acid
keywords: Fatty acids; Heterotrophy; Microalgae; Mixotrophy; Pigments; Wood hydrolysate