title: Fusion of flagellin 2 with bivalent white spot syndrome virus vaccine increases survival in freshwater shrimp.
abstract: Despite large economic losses attributable to white spot syndrome virus (WSSV), an infectious pathogen of penaeid shrimp and other crustaceans worldwide, no efficient vaccines or antiviral agents to control the virus are available at present. Here, we designed and constructed baculovirus-based vaccines delivering genes encoding the WSSV envelope proteins, VP28 and VP19. To enhance the immunogenicity of the baculovirus-based vaccine, we fused a Salmonella typhimurium flagellin 2 (FL2) gene with VP28 or VP19 gene. Both vaccine constructs elicited similar high titlers of anti-WSSV IgG after oral immunization in mice. The protective effect of oral vaccines upon WSSV challenge was observed in Macrobrachium nipponense. Bivalent vaccine displaying WSSV envelope proteins, VP19 and VP28, led to enhanced more than 10% survival protection against WSSV infection, compared to monovalent vaccine containing WSSV envelope protein, VP19 or VP28. Furthermore, a baculovirus-based WSSV vaccine fused with FL2 gene, Ac-VP28-ie1VP19FL2, efficiently protected mice against WSSV challenge (89.5% survival rate). In support of the efficacy of FL2 in our vaccine, we verified FL2 enhanced survival rate and induced the NF-κB gene in Palaemon paucidens. The collective results strongly suggest that our recombinant baculoviral system displaying WSSV envelope protein and delivering FL2-fused WSSV envelope gene effectively induced protective responses, supporting the utility of a potential new oral DNA vaccine against WSSV.
authors.affiliation: Department of Bio-industrial Technologies, Konkuk University, 120, Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea.
authors.forename: Hansam
authors.initials: H
authors.lastname: Cho
authors.affiliation: Department of Microbiology, Pukyong National University, 45, Yongso-ro, Nam-Gu, Busan 48513, Republic of Korea.
authors.forename: Na Hye
authors.initials: NH
authors.lastname: Park
authors.affiliation: Department of Bio-industrial Technologies, Konkuk University, 120, Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea.
authors.forename: Yuyeon
authors.initials: Y
authors.lastname: Jang
authors.affiliation: Department of Bio-industrial Technologies, Konkuk University, 120, Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea.
authors.forename: Yong-Dae
authors.initials: YD
authors.lastname: Gwon
authors.affiliation: Department of Bio-industrial Technologies, Konkuk University, 120, Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea.
authors.forename: Yeondong
authors.initials: Y
authors.lastname: Cho
authors.affiliation: Department of Bio-industrial Technologies, Konkuk University, 120, Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea.
authors.forename: Yoon-Ki
authors.initials: YK
authors.lastname: Heo
authors.affiliation: Department of Bio-industrial Technologies, Konkuk University, 120, Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea.
authors.forename: Ki-Hoon
authors.initials: KH
authors.lastname: Park
authors.affiliation: Department of Bio-industrial Technologies, Konkuk University, 120, Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea.
authors.forename: Hee-Jung
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Microbiology, Pukyong National University, 45, Yongso-ro, Nam-Gu, Busan 48513, Republic of Korea.
authors.forename: Tae Jin
authors.initials: TJ
authors.lastname: Choi
authors.affiliation: Department of Bio-industrial Technologies, Konkuk University, 120, Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. Electronic address: kimera@konkuk.ac.kr.
authors.forename: Young Bong
authors.initials: YB
authors.lastname: Kim
mesh_terms: D000818:Animals; D005408:Flagellin / Q000276:immunology; D033561:Penaeidae / Q000821:virology*; D014759:Viral Envelope Proteins / Q000276:immunology / Q000494:pharmacology; D014765:Viral Vaccines; D046848:White spot syndrome virus 1
chemical_list: D014759:Viral Envelope Proteins; D014765:Viral Vaccines; D005408:Flagellin
keywords: Baculovirus; DNA vaccine; FL2; Innate immunity; Shrimp; White spot syndrome virus