title: Passiflora tarminiana fruits reduce UVB-induced photoaging in human skin fibroblasts.
abstract: Skin aging is a complex process that is strongly affected by UV radiation, which stimulates the production of reactive oxygen species (ROS) in the epidermis and dermis and subsequently causes skin damage. Among the major consequences are increased collagen degradation and reduced collagen synthesis. Previous reports have demonstrated the beneficial effects of polyphenols for healthy skin. Passiflora tarminiana Coppens & V.E. Barney, a species of the Passifloraceae family, is widely distributed in South America and is rich in flavonoids. We show that UVB radiation increases metalloproteinase 1 (MMP-1) and reduces procollagen production in human dermal fibroblast (HDF) cells in a dose- and time-dependent manner. We examined the antioxidant and antiaging effects of the extract and fractions of P. tarminiana fruits. The fractions showed high polyphenol content (620mg EAG/g) and antioxidant activity, as measured by ORAC (4097μmol ET/g) and ABTS (2992μmol ET/g) assays. The aqueous fraction drastically inhibited the collagenase enzyme (IC500.43μg/mL). The extract and fractions presented photoprotective effects by reducing UVB-induced MMP-1 production, increasing UVB-inhibited procollagen production, and decreasing ROS production after UVB irradiation in HDF. Finally, the polyphenol contents of the extracts and fractions from P. tarminiana were analyzed by HPLC-DAD-ESI-MSn, and procyanidins and glycosylated flavonoids were identified.
authors.affiliation: Grupo de investigación en Sustancias Bioactivas, Facultad de Ciencias Farmacéuticas y Alimentarias, Universidad de Antioquia, Calle 70 No. 52-21, Medellín, Colombia.
authors.forename: Karent
authors.initials: K
authors.lastname: Bravo
authors.affiliation: Grupo de investigación en Sustancias Bioactivas, Facultad de Ciencias Farmacéuticas y Alimentarias, Universidad de Antioquia, Calle 70 No. 52-21, Medellín, Colombia.
authors.forename: Luisa
authors.initials: L
authors.lastname: Duque
authors.affiliation: CEBAS-CSIC, Food Sci. & Technol. Dept., Research Group on Quality, Safety and Bioactivity of Plant Foods, Campus Universitario de Espinardo - 25, Espinardo, 30100 Murcia, Spain.
authors.forename: Federico
authors.initials: F
authors.lastname: Ferreres
authors.affiliation: CEBAS-CSIC, Food Sci. & Technol. Dept., Research Group on Quality, Safety and Bioactivity of Plant Foods, Campus Universitario de Espinardo - 25, Espinardo, 30100 Murcia, Spain.
authors.forename: Diego A
authors.initials: DA
authors.lastname: Moreno
authors.affiliation: Grupo de investigación en Sustancias Bioactivas, Facultad de Ciencias Farmacéuticas y Alimentarias, Universidad de Antioquia, Calle 70 No. 52-21, Medellín, Colombia. Electronic address: edison.osorio@udea.edu.co.
authors.forename: Edison
authors.initials: E
authors.lastname: Osorio
mesh_terms: D000872:Anthocyanins / Q000032:analysis; D000975:Antioxidants / Q000494:pharmacology; D002478:Cells, Cultured; D024042:Collagen Type I / Q000187:drug effects / Q000378:metabolism; D005347:Fibroblasts / Q000528:radiation effects*; D005419:Flavonoids / Q000032:analysis; D005638:Fruit / Q000737:chemistry; D006801:Humans; D029598:Passiflora; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D015595:Skin Aging / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D024042:Collagen Type I; D005419:Flavonoids; D010936:Plant Extracts; D017382:Reactive Oxygen Species
keywords: HPLC-ESI-MS(n); Human fibroblasts; Passiflora; Skin aging; UVB radiation