title: NMR and ESR study of amphotericin B interactions with various binary phosphatidylcholine/phosphatidylglycerol membranes.
abstract: Several biologically relevant phospholipids are considered as potential excipients for IV administration liposome's formulation of AMB (Biopharmaceututics Classification System Class IV). On the basis of in vivo bioavaibility studies, DMPC and DMPG were ranked as the first potent encapsulation enhancers for this model drug, especially if one expects to target DMPG rich systems as pulmonary surfactant. Subsequently, dispersions (multilayers) of DMPC, DMPG or in binary systems with various molar ratios were prepared with or without AMB (molar ratios AMB/lipid) and further investigated using the1H-,31P-NMR methods. It was found that equimolar preparations of DMPG/DMPG exhibited both a good encapsulation of AMB, while also probably able to target pulmonary surfactant. Besides DMPG did not exhibit the same solubilization properties. Conversely, no targeting by DMPC dispersion alone was expected, even if a good solubilization was obtained.
authors.affiliation: Institut de Recherches Biomédicales du Service de Santé des Armées, 91223 Bretigny, France; CLINATEC, centre de recherche biomédicale Edmond j. Safra, commissariat à l'énergie atomique et aux énergies alternatives, 17 rue des Martyrs, 38000 Grenoble, France. Electronic address: jcddebouzy@gmail.com.
authors.forename: J C
authors.initials: JC
authors.lastname: Debouzy
authors.affiliation: Normandie Université, univ Rouen, Lab Pharmacie Galénique, DC2N INSERM U 982, 22 Bd Gambetta, 76183 Rouen, France.
authors.forename: L
authors.initials: L
authors.lastname: Mehenni
authors.affiliation: Institut de Recherches Biomédicales du Service de Santé des Armées, 91223 Bretigny, France; CLINATEC, centre de recherche biomédicale Edmond j. Safra, commissariat à l'énergie atomique et aux énergies alternatives, 17 rue des Martyrs, 38000 Grenoble, France.
authors.forename: D
authors.initials: D
authors.lastname: Crouzier
authors.affiliation: Normandie Université, univ Rouen, Lab Pharmacie Galénique, DC2N INSERM U 982, 22 Bd Gambetta, 76183 Rouen, France.
authors.forename: M
authors.initials: M
authors.lastname: Lahiani-Skiba
authors.affiliation: Institut de Recherches Biomédicales du Service de Santé des Armées, 91223 Bretigny, France.
authors.forename: G
authors.initials: G
authors.lastname: Nugue
authors.affiliation: Normandie Université, univ Rouen, Lab Pharmacie Galénique, DC2N INSERM U 982, 22 Bd Gambetta, 76183 Rouen, France.
authors.forename: M
authors.initials: M
authors.lastname: Skiba
mesh_terms: D000666:Amphotericin B / Q000737:chemistry*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010715:Phosphatidylglycerols / Q000737:chemistry*; D012995:Solubility
chemical_list: D010715:Phosphatidylglycerols; D000666:Amphotericin B; C002773:dimyristoylphosphatidylglycerol; D004134:Dimyristoylphosphatidylcholine
keywords: Amphotericin B; DMPC; DMPG; ESR; Formulation; Membranes; NMR; Phospholipids ratios; Solubilization