title: Immobilization of Irpex lacteus to liquid-core alginate beads and their application to degradation of pollutants.
abstract: White rot fungi (WRF) are applicable to biodegradation of recalcitrant pollutants. However, excessive biomass growth typical for WRF cultivation can hinder their large scale applications. Therefore, immobilization of Irpex lacteus to liquid-core alginate beads restricting excessive mycelium growth and simultaneously keeping high degradation rate of pollutants was tested. Effective diffusivities of dyes to the beads varied from (2.98 ± 0.69) × 10-10to (10.27 ± 2.60) × 10-10m2/s. Remazol Brilliant Blue R (RBBR), Reactive Orange 16 (RO16), and Naphthol Blue Black (NBB) were used as model dyes. The immobilized fungus decolorized model dyes when applied both in microwell plates and in fluidized bed reactors. Using the microwell plates, the apparent reaction rate constants ranged from (2.06 ± 0.11) × 10-2to (11.06 ± 0.27) × 10-21/h, depending on the dye used and its initial concentration. High initial concentrations negatively affected the dye decolorization rate. No fungal growth outside the beads was observed in fluidized bed reactors and thus no operational problems linked to an excessive biomass growth occurred. When RBBR was decolorized in subsequent batches in the fluidized bed reactor, the apparent reaction rate constant increased from (11.63 ± 0.35) × 10-2to (29.26 ± 7.19) × 10-21/h.
authors.affiliation: Department of Chemical Engineering, University of Chemistry and Technology, Prague, Technická 5, 16628, Prague 6, Czech Republic. siman@vscht.cz.
authors.forename: Jan
authors.initials: J
authors.lastname: Šíma
authors.affiliation: Department of Chemical Engineering, University of Chemistry and Technology, Prague, Technická 5, 16628, Prague 6, Czech Republic.
authors.forename: Rachel
authors.initials: R
authors.lastname: Milne
authors.affiliation: Institute of Microbiology ASCR, Vídeňská 1083, 142 20, Prague 4, Czech Republic.
authors.forename: Čeněk
authors.initials: Č
authors.lastname: Novotný
authors.affiliation: Department of Chemical Engineering, University of Chemistry and Technology, Prague, Technická 5, 16628, Prague 6, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Hasal
mesh_terms: D000464:Alginates / Q000737:chemistry; D001673:Biodegradation, Environmental; D018914:Cells, Immobilized / Q000737:chemistry / Q000378:metabolism; D004396:Coloring Agents / Q000378:metabolism*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D025282:Mycelium / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism; D020072:Polyporales / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D000464:Alginates; D004396:Coloring Agents; D006603:Hexuronic Acids; D014874:Water Pollutants, Chemical; D020723:Glucuronic Acid