title: Downregulation of OCLN and GAS1 in clear cell renal cell carcinoma.
abstract: Clear cell renal cell carcinoma (ccRCC) is the most common histological subtype of kidney cancer. This carcinoma is histologically characterized by the presence of clear and abundant cytoplasm. In the present study, we sought to identify genes differentially expressed in ccRCC and build a molecular profile of this cancer. We selected genes described in the literature related to cellular differentiation and proliferation. We analyzed the gene and protein expression by quantitative PCR (qPCR) and immunohistochemistry, respectively, and examined possible epigenetic mechanisms that regulate their expression in ccRCC samples and cell lines. Occludin (OCLN) and growth arrest-specific 1 (GAS1) genes were underexpressed in ccRCC, and we report that miR-122 and miR-34a, respectively, may regulate their expression in this cancer. Furthermore, we showed by qPCR and immunohistochemistry that solute carrier family 2 member 1 (SLC2A1) was significantly overexpressed in ccRCC. The set of genes identified in the present study furthers our understanding of the molecular basis and development of ccRCC.
authors.affiliation: Laboratory of Genomic Studies, Department of Biology, São Paulo State University (UNESP), São José do Rio Preto, SP 15054-000, Brazil.
authors.forename: André Luis Giacometti
authors.initials: AL
authors.lastname: Conceição
authors.affiliation: Department of Pharmacology, The Federal University of São Paulo (UNIFESP), São Paulo, SP 15054-000, Brazil.
authors.forename: Camila Tainah
authors.initials: CT
authors.lastname: Da Silva
authors.affiliation: Laboratory of Genomic Studies, Department of Biology, São Paulo State University (UNESP), São José do Rio Preto, SP 15054-000, Brazil.
authors.forename: Rodolfo Miglioli
authors.initials: RM
authors.lastname: Badial
authors.affiliation: Laboratory of Genomic Studies, Department of Biology, São Paulo State University (UNESP), São José do Rio Preto, SP 15054-000, Brazil.
authors.forename: Marina Curado
authors.initials: MC
authors.lastname: Valsechi
authors.affiliation: Laboratory of Genomic Studies, Department of Biology, São Paulo State University (UNESP), São José do Rio Preto, SP 15054-000, Brazil.
authors.forename: Bruna
authors.initials: B
authors.lastname: Stuqui
authors.affiliation: Department of Pharmacology, The Federal University of São Paulo (UNIFESP), São Paulo, SP 15054-000, Brazil.
authors.forename: Jéssica Domingues
authors.initials: JD
authors.lastname: Gonçalves
authors.affiliation: Department of Pharmacology, The Federal University of São Paulo (UNIFESP), São Paulo, SP 15054-000, Brazil.
authors.forename: Miriam Galvonas
authors.initials: MG
authors.lastname: Jasiulionis
authors.affiliation: Laboratory of Genomic Studies, Department of Biology, São Paulo State University (UNESP), São José do Rio Preto, SP 15054-000, Brazil.
authors.forename: Marilia
authors.initials: M
authors.lastname: De Freitas Calmon
authors.affiliation: Laboratory of Genomic Studies, Department of Biology, São Paulo State University (UNESP), São José do Rio Preto, SP 15054-000, Brazil.
authors.forename: Paula
authors.initials: P
authors.lastname: Rahal
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D014408:Biomarkers, Tumor; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D047369:Chromatin Immunoprecipitation; D005260:Female; D058851:GPI-Linked Proteins / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D007680:Kidney Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D062793:Occludin / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; C096192:GAS1 protein, human; D058851:GPI-Linked Proteins; C531907:MIRN122 microRNA, human; C522425:MIRN34 microRNA, human; D035683:MicroRNAs; C568985:OCLN protein, human; D062793:Occludin; D012333:RNA, Messenger