title: LIFE BEYOND LIFE - An Easy Way to Derive Lung Fibroblasts from Cadavers.
abstract: Several protocols have illustrated the possibility of deriving cells, such as fibroblasts, from different organs. These techniques generally concern organs sampled from living persons, but have already been described for cadavers, especially concerning the skin and tendons. We present, for the first time, an easy way to derive pulmonary fibroblasts from a lung tissue sampled from a cadaver and directly culture plated. The fibroblast output was checked daily. We obtained lung fibroblasts from 3 (60%) cadavers and 2 (100%) living persons. The fibroblast output took about 3 days for cells from living persons and took up to 39 days for those from cadavers. We did not clearly identify any parameters that could explain these differences. Nevertheless, these derived cells had the same features as the source cells, especially in terms of morphology and proliferation, and could potentially be used in different research domains such as forensic or regeneration medicine.
authors.affiliation: Emergency Pole, Forensic Medicine Department, Montpellier University Hospital, 371 Avenue du Doyen Gaston Giraud, Montpellier, 34285, France.
authors.forename: Sophie
authors.identifier: http://orcid.org/0000-0001-6948-120X
authors.initials: S
authors.lastname: Colomb
authors.affiliation: Molecular Genetics Laboratory - IURC, Montpellier University Hospital, 641 Avenue du Doyen Gaston Giraud, Montpellier, 34093, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Bareil
authors.affiliation: Emergency Pole, Forensic Medicine Department, Montpellier University Hospital, 371 Avenue du Doyen Gaston Giraud, Montpellier, 34285, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Baccino
authors.affiliation: Heart-Lung Pole, Cardiac, Thoracic and Vascular Surgery, Montpellier University Hospital, 375 Avenue du Doyen Gaston Giraud, Montpellier, 34295, France.
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Berthet
authors.affiliation: Heart-Lung Pole, Department of Lung Diseases and Addictology, Montpellier University Hospital, 375 Avenue du Doyen Gaston Giraud, Montpellier, 34295, France.
authors.forename: Lucie
authors.initials: L
authors.lastname: Knabe
authors.affiliation: Heart-Lung Pole, Department of Lung Diseases and Addictology, Montpellier University Hospital, 375 Avenue du Doyen Gaston Giraud, Montpellier, 34295, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Vachier
authors.affiliation: Heart-Lung Pole, Department of Lung Diseases and Addictology, Montpellier University Hospital, 375 Avenue du Doyen Gaston Giraud, Montpellier, 34295, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Bourdin
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002102:Cadaver; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D016172:DNA Fingerprinting; D005260:Female; D005347:Fibroblasts / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008168:Lung / Q000166:cytology*; D008297:Male; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction; D055815:Young Adult
keywords: explant; fibroblast; forensic autopsy; forensic science; lung; postmortem delay; senescence