title: Effect of artemisinin on neuropathic pain mediated by P2X4receptor in dorsal root ganglia.
abstract: Neuropathic pain is a type of chronic pain caused by nervous system damage and dysfunction. The pathogenesis of chronic pain is complicated, and there are no effective therapies for neuropathic pain. Studies show that the P2X4receptor expressed in the satellite glial cells (SGCs) of dorsal root ganglia (DRG) is related to neuropathic pain. Artemisinin is a monomeric component extracted from traditional Chinese medicine and has a variety of important pharmacological effects and potential applications. This study observed the effect of artemisinin on neuropathic pain and delineated its possible mechanism. The chronic constriction injury (CCI) rat model was used in this study. The results demonstrated that artemisinin relieved pain behaviors in the CCI rats, inhibited the expression of P2X4receptor in the DRG, and decreased the ATP-activated currents in HEK293 cells transfected with P2X4plasmid. Dual-labeling immunofluorescence showed that the coexpression of P2X4receptor and glial fibrillary acidic protein (GFAP) in the DRG of CCI rats was increased compared to control rats. After CCI rats were treated with artemisinin, the coexpression of P2X4receptor and GFAP in the DRG was significantly decreased compared to the CCI group. This finding suggested that artemisinin could inhibit the nociceptive transmission mediated by P2X4receptor in the DRG SGCs and thus relieve pain behaviors in the CCI rats.
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang 330006, PR China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang 330006, PR China.
authors.forename: Mofeng
authors.initials: M
authors.lastname: Ying
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang 330006, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of English Language Teaching, Nanchang Institute of Science and Technology, Nanchang, Jiangxi 330006, PR China.
authors.forename: Tengling
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Class 131, Queen Marie College of Nanchang University, Medical College of Nanchang University, Nanchang, 330008, PR China.
authors.forename: Chenxu
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Department of the First Clinical Medicine, Medical College of Nanchang University, Nanchang, 330008, PR China.
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Gong
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang 330006, PR China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang 330006, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang 330006, PR China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang 330006, PR China.
authors.forename: Lifang
authors.initials: L
authors.lastname: Zou
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang 330006, PR China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang 330006, PR China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Yi
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang 330006, PR China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang 330006, PR China.
authors.forename: Shenqiang
authors.initials: S
authors.lastname: Rao
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang 330006, PR China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang 330006, PR China.
authors.forename: Guilin
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Medical Genetics and Biology, Medical College of Nanchang University, Nanchang 330006, PR China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang 330006, PR China.
authors.forename: Chunping
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang 330006, PR China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang 330006, PR China.
authors.forename: Tianyu
authors.initials: T
authors.lastname: Jia
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang 330006, PR China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang 330006, PR China.
authors.forename: Shanhong
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang 330006, PR China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang 330006, PR China.
authors.forename: Huilong
authors.initials: H
authors.lastname: Yuan
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang 330006, PR China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang 330006, PR China.
authors.forename: Liran
authors.initials: L
authors.lastname: Shi
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang 330006, PR China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang 330006, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang 330006, PR China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang 330006, PR China. Electronic address: liangsd@hotmail.com.
authors.forename: Shangdong
authors.initials: S
authors.lastname: Liang
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang 330006, PR China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang 330006, PR China. Electronic address: liushuangmei_1983@163.com.
authors.forename: Shuangmei
authors.initials: S
authors.lastname: Liu
mesh_terms: D000818:Animals; D037621:Artemisinins / Q000494:pharmacology / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D005727:Ganglia, Spinal / Q000187:drug effects / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D009437:Neuralgia / Q000188:drug therapy* / Q000378:metabolism*; D010147:Pain Measurement / Q000187:drug effects / Q000379:methods*; D058920:Purinergic P2X Receptor Antagonists / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D058848:Receptors, Purinergic P2X4 / Q000502:physiology*; D016896:Treatment Outcome
chemical_list: D037621:Artemisinins; D058920:Purinergic P2X Receptor Antagonists; D058848:Receptors, Purinergic P2X4; C031327:artemisinin
keywords: Artemisinin; Dorsal root ganglia; Neuropathic pain; P2X(4) receptor; Satellite glial cell