title: T cell immunoregulation in active ocular toxoplasmosis.
abstract: Toxoplasma gondii infection is an important cause of infectious ocular disease. The physiopathology of retinochoroidal lesions associated with this infection is not completely understood. The present study was undertaken to investigate cytokine production by T cells from individuals with active toxoplasmic retinochoroiditis (TR) comparing with controls. Eighteen patients with active TR and 15 healthy controls (6 controls IgG+to Toxoplasma and 9 negative controls) were included in the study. Peripheral blood mononuclear cells were incubated in the presence or absence of T. gondii antigen (STAg), and stained against CD4, CD8, TNF, IL-10 and IFN-γ. Baseline expression of cytokines was higher in TR/IgG+patients in comparison with controls. Cytokine expression was not increased by STAg in vitro stimulation in controls. After stimulation, TR/IgG+patients' lymphocytes increased cytokine as compared to cultures from both controls. While T cells were the main source of IL-10, but also IFN-γ and TNF, other lymphocyte populations were relevant source of inflammatory cytokines. Interestingly, it was observed a negative correlation between ocular lesion size and IL-10 expression by CD4+lymphocytes. This study showed that T cells are the main lymphocyte populations expressing IL-10 in patients with TR. Moreover, expression of IL-10 plays a protective role in active TR.
authors.affiliation: Interdisciplinary Laboratory of Medical Investigation, School of Medicine, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil; Centro Brasileiro de Ciências Visuais, Belo Horizonte, MG, Brazil; Retina Department, Massachusetts Eye and Ear Infirmary, Boston, MA, USA.
authors.forename: Cynthia A
authors.initials: CA
authors.lastname: Cordeiro
authors.affiliation: Interdisciplinary Laboratory of Medical Investigation, School of Medicine, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. Electronic address: ericalmvieira@gmail.com.
authors.forename: Erica L M
authors.initials: ELM
authors.lastname: Vieira
authors.affiliation: Interdisciplinary Laboratory of Medical Investigation, School of Medicine, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil; Centro Brasileiro de Ciências Visuais, Belo Horizonte, MG, Brazil.
authors.forename: Vinicius M
authors.initials: VM
authors.lastname: Castro
authors.affiliation: Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Walderez O
authors.initials: WO
authors.lastname: Dutra
authors.affiliation: Centro Brasileiro de Ciências Visuais, Belo Horizonte, MG, Brazil.
authors.forename: Rogerio A
authors.initials: RA
authors.lastname: Costa
authors.affiliation: Centro Brasileiro de Ciências Visuais, Belo Horizonte, MG, Brazil.
authors.forename: Juliana L
authors.initials: JL
authors.lastname: Orefice
authors.affiliation: Departamento de Oftalmologia, Setor de Uveítes, Hospital São Geraldo, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Wesley R
authors.initials: WR
authors.lastname: Campos
authors.affiliation: Centro Brasileiro de Ciências Visuais, Belo Horizonte, MG, Brazil.
authors.forename: Fernando
authors.initials: F
authors.lastname: Orefice
authors.affiliation: Retina Department, Massachusetts Eye and Ear Infirmary, Boston, MA, USA.
authors.forename: Lucy H
authors.initials: LH
authors.lastname: Young
authors.affiliation: Interdisciplinary Laboratory of Medical Investigation, School of Medicine, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil; Department of Psychiatry and Behavioral Sciences, UT Health, Houston, TX, USA. Electronic address: altexr@gmail.co.
authors.forename: Antonio Lucio
authors.initials: AL
authors.lastname: Teixeira
mesh_terms: D000293:Adolescent; D000328:Adult; D000909:Antibodies, Helminth / Q000276:immunology; D016022:Case-Control Studies; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D056747:Immunomodulation; D018836:Inflammation Mediators; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D008875:Middle Aged; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D014122:Toxoplasma / Q000276:immunology*; D014126:Toxoplasmosis, Ocular / Q000175:diagnosis / Q000276:immunology* / Q000378:metabolism / Q000469:parasitology*; D055815:Young Adult
chemical_list: D000909:Antibodies, Helminth; D016207:Cytokines; D007074:Immunoglobulin G; D018836:Inflammation Mediators
keywords: Cytokines; T lymphocytes; Toxoplasmosis; Uveitis