title: Newly Formed Bone Induced by Recombinant Human Bone Morphogenetic Protein-2: A Histological Observation.
abstract: OBJECTIVE
  To observe, histologically, bone induced by recombinant human bone morphogenetic protein-2 (rhBMP-2) in onlay grafted and sinus lifted alveolaris.
  
  
  METHODS
  Eighteen patients were treated with rhBMP-2 at concentration 1.5 mg/mL with an absorbable collagen sponge (ACS). The treated bone was harvested with small trephine bur at 5 or 7 months after surgery for the micro Computer Scanning (CT) and light microscopic observation.
  
  
  RESULTS
  Micro CT showed clearly 3-dimensional trabecular bone structure. New bone formation and bone marrow structure were observed in the observed area. Osteoblastic cells existed along the new bone, and osteopontin was localized in the bone matrix weakly. In the connective tissue around the new bone, many CD34-positive blood vessel cells were present. Some tartrate-resistant acid phosphatase (TRAP)-positive osteoclastic cells were observed around bone at this stage.
  
  
  CONCLUSIONS
  The application of rhBMP-2 with ACS induced a new bone accompanied by blood vessels in atrophied alveolaris. This suggests that rhBMP-2 is capable of osteoinductivity in human jaw.
authors.affiliation: *Assistant Professor, Department of Anatomy and Cell Biology, Osaka Medical College, Takatsuki, Japan. †Professor and Chairman, Department of Dentistry and Oral Surgery, Osaka Medical College, Takatsuki, Japan. ‡Professor and Chairman, Surgical Implant Dentistry, University of California, Los Angeles, CA.
authors.forename: Azumi
authors.initials: A
authors.lastname: Hirata
authors.forename: Takaaki
authors.initials: T
authors.lastname: Ueno
authors.forename: Peter K
authors.initials: PK
authors.lastname: Moy
mesh_terms: D000539:Alveolar Process / Q000033:anatomy & histology / Q000737:chemistry / Q000000981:diagnostic imaging / Q000254:growth & development*; D001857:Bone Matrix / Q000033:anatomy & histology / Q000000981:diagnostic imaging / Q000254:growth & development; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D006801:Humans; D053495:Osteopontin / Q000032:analysis; D011994:Recombinant Proteins / Q000494:pharmacology; D059546:Sinus Floor Augmentation / Q000379:methods; D055114:X-Ray Microtomography
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D011994:Recombinant Proteins; D053495:Osteopontin