title: The effects of graded levels of calorie restriction: VIII. Impact of short term calorie and protein restriction on basal metabolic rate in the C57BL/6 mouse.
abstract: Under calorie restriction (CR) animals need to lower energy demands. Whether this involves a reduction in cellular metabolism is an issue of contention. We exposed C57BL/6 mice to graded CR for 3 months, measured BMR and dissected out 20 body compartments. From a separate age-matched group (n=57), we built 7 predictive models for BMR. Unadjusted BMR declined with severity of restriction. Comparison of measured and predicted BMR from the simple models suggested suppression occurred. The extent of 'suppression' was greater with increased CR severity. However, when models based on individual organ sizes as predictors were used, the discrepancy between the prediction and the observed BMR disappeared. This suggested 'metabolic suppression' was an artefact of not having a detailed enough model to predict the expected changes in metabolism. Our data have wide implications because they indicate that inferred 'metabolic' impacts of genetic and other manipulations may reflect effects on organ morphology.
authors.affiliation: Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, Scotland, UK.
authors.forename: Sharon E
authors.initials: SE
authors.lastname: Mitchell
authors.affiliation: Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, Scotland, UK.
authors.forename: ZhanHui
authors.initials: Z
authors.lastname: Tang
authors.affiliation: Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, Scotland, UK.
authors.forename: Celine
authors.initials: C
authors.lastname: Kerbois
authors.affiliation: Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, Scotland, UK.
authors.forename: Camille
authors.initials: C
authors.lastname: Delville
authors.affiliation: Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, Scotland, UK.
authors.forename: Davina
authors.initials: D
authors.lastname: Derous
authors.affiliation: Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, Scotland, UK.
authors.forename: Cara L
authors.initials: CL
authors.lastname: Green
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Computational Biology, Chinese Academy of Sciences-Max Planck Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Jackie J D
authors.initials: JJ
authors.lastname: Han
authors.affiliation: Key Laboratory of Systems Biology, Innovation Center for Cell Signaling Network, Institute of Biochemistry and Cell Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Luonan
authors.initials: L
authors.lastname: Chen
authors.affiliation: Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, Scotland, UK.
authors.forename: Alex
authors.initials: A
authors.lastname: Douglas
authors.affiliation: Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, Scotland, UK.
authors.forename: David
authors.initials: D
authors.lastname: Lusseau
authors.affiliation: Department of Pathology, University of Washington, Seattle, Washington, USA.|Department of Biology, University of Washington, Seattle, Washington, USA.
authors.forename: Daniel E L
authors.initials: DE
authors.lastname: Promislow
authors.affiliation: Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, Scotland, UK.|State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors.forename: John R
authors.initials: JR
authors.lastname: Speakman
mesh_terms: D000818:Animals; D001481:Basal Metabolism / Q000502:physiology*; D031204:Caloric Restriction; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D015233:Models, Statistical; D009929:Organ Size
keywords: Gerotarget; calorie restriction; dietary restriction; metabolic rate; metabolic suppression; protein restriction