title: Simultaneous domestic wastewater and nitrate sewage treatment by DEnitrifying AMmonium OXidation (DEAMOX) in sequencing batch reactor.
abstract: A novel DEAMOX system was developed for nitrogen removal from domestic wastewater and nitrate (NO3--N) sewage in sequencing batch reactor (SBR). High nitrite (NO2--N) was produced from NO3--N reduction in partial-denitrification process, which served as electron acceptor for anammox and was removed with ammonia (NH4+-N) in domestic wastewater simultaneously. A 500-days operation demonstrated that the efficient and stable nitrogen removal performance could be achieved by DEAMOX. The total nitrogen (TN) removal efficiency was as high as 95.8% with influent NH4+-N of 63.58 mg L-1and NO3--N of 69.24 mg L-1. The maximum NH4+-N removal efficiency reached up to 94.7%, corresponding to the NO3--N removal efficiency of 97.8%. The biomass of partial-denitrification and anammox bacteria was observed to be wall-growth. The deteriorated nitrogen removal performance occurred due to excess denitrifying microbial growth in the outer layer of sludge consortium, which prevented the substrate transfer for anammox inside. However, an excellent nitrogen removal could be guaranteed by scrapping the superficial denitrifying biomass at regular intervals. Furthermore, the high-throughput sequencing analysis revealed that the Thauera genera (26.33%) was possibly responsible for the high NO2--N accumulation in partial-denitrification and Candidatus Brocadia (1.7%) was the major anammox species.
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Du
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Shenbin
authors.initials: S
authors.lastname: Cao
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China.
authors.forename: Baikun
authors.initials: B
authors.lastname: Li
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China.
authors.forename: Shuying
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China. Electronic address: pyz@bjut.edu.cn.
authors.forename: Yongzhen
authors.initials: Y
authors.lastname: Peng
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D058440:Denitrification; D010084:Oxidation-Reduction; D012722:Sewage / Q000737:chemistry* / Q000382:microbiology; D062065:Wastewater / Q000737:chemistry* / Q000382:microbiology; D018508:Water Purification / Q000379:methods*
chemical_list: D000641:Ammonia; D012722:Sewage; D062065:Wastewater
keywords: Anammox; Domestic wastewater; High-throughput sequencing; Nitrite accumulation; Partial denitrification