title: Antimutagenic Effect ofHibiscus sabdariffaL. Aqueous Extract on Rats Treated with Monosodium Glutamate.
abstract: Hibiscus sabdariffaL. is a plant of the Malvaceae family, commonly known as roselle.H. sabdariffais known to contain antioxidant, cholesterol-lowering, antiobesity, insulin resistance reduction, antihypertensive, and skin cancer chemopreventive properties. This study evaluated the effects ofH. sabdariffaaqueous extract against cyclophosphamide (CPA, 25 mg/Kg) induced damage to DNA in male Wistar rats by micronucleus test. Samples ofH. sabdariffacalyx were obtained in the municipality of Barra do Garças, Mato Grosso, Brazil. The aqueous extract was prepared by infusion and each animal received a daily dose of 400 mg/Kg by gavage for 15 consecutive days of treatment. The presence of anthocyanins was confirmed by ferric chloride test and phenolic compounds using high-performance liquid chromatography, with emphasis on the identification of rutin. The animals were sacrificed by deepening of anaesthesia to obtain bone marrow and determination of the frequency of micronucleated polychromatic erythrocytes. The group treated with the aqueous extract ofH. sabdariffarevealed a 91% reduction in micronucleus frequency when compared with the positive control group. Under the conditions tested,H. sabdariffaL. presented a protective effect to CPA-induced damage to DNA of the treated animals, and it is a potential candidate as a chemopreventive agent against carcinogenesis.
authors.affiliation: Institute of Natural, Humanities and Social Sciences, Federal University of Mato Grosso, Sinop, MT, Brazil.
authors.forename: Ana Carla Guidini Valentini
authors.identifier: 0000-0003-2566-4156
authors.initials: AC
authors.lastname: Gheller
authors.affiliation: Institute of Natural, Humanities and Social Sciences, Federal University of Mato Grosso, Sinop, MT, Brazil.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Kerkhoff
authors.affiliation: Department of Chemistry, Federal University of Piauí, Teresina, PI, Brazil.
authors.forename: Gerardo Magela
authors.initials: GM
authors.lastname: Vieira Júnior
authors.affiliation: Institute of Biological and Health Sciences, Federal University of Mato Grosso, Barra do Garças, MT, Brazil.
authors.forename: Kleber Eduardo
authors.initials: KE
authors.lastname: de Campos
authors.affiliation: Institute of Health Sciences, Federal University of Mato Grosso, Sinop, MT, Brazil.
authors.forename: Marina Mariko
authors.initials: MM
authors.lastname: Sugui
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001938:Brazil; D004249:DNA Damage; D031584:Hibiscus / Q000737:chemistry*; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012970:Sodium Glutamate
chemical_list: D016587:Antimutagenic Agents; D010936:Plant Extracts; D012970:Sodium Glutamate