title: Tanshinone IIA suppresses gastric cancer cell proliferation and migration by downregulation of FOXM1.
abstract: Tanshinone IIA (TSN) exhibits a variety of anticancer effects. However, whether it inhibits gastric cancer (GC) cell proliferation and migration and the mechanism remain unclear. In the present study, different concentrations of TSN were co-incubated with SGC-7901 cells. The pcDNA-FOXM1 or FOXM1-siRNA plasmid was transfected into cells before treatment with 5 µg/l TSN. The proliferation and migration abilities of the SGC-7901 cells were tested by MTT and wound healing assays. Western blotting was used to investigate the expression levels of P21, Ki-67, PCNA, MMP-2, MMP-9 and FOXM1. We found that compared with the control, the proliferation and migration abilities of the SGC-7901 cells were decreased after incubation with different concentrations of TSN in a dose-dependent manner (p<0.01). Moreover, the expression levels of Ki-67, PCAN, MMP-2, MMP-9 and FOXM1 were decreased, and P21 was increased in the TSN-treated SGC-7901 cells (p<0.01). In addition, downregulation of FOXM1 by FOXM1-siRNA had the same effect as TSN on SGC-7901 cells, and overexpression of FOXM1 partly abrogated TSN-mediated inhibition of SGC-7901 cell proliferation and migration. These results suggested that TSN inhibits SGC-7901 cell proliferation and migration by downregulation of FOXM1.
authors.affiliation: Linyi Hospital of Traditional Chinese Medicine, Linyi, Shandong 276000, P.R. China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Yu
authors.affiliation: Linyi Tumor Hospital, Linyi, Shandong 276000, P.R. China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Wang
authors.affiliation: Linyi Hospital of Traditional Chinese Medicine, Linyi, Shandong 276000, P.R. China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Li
authors.affiliation: Lanzhou Hengdao Chinese Medicine Institute, Lanzhou, Shandong 730000, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Tang
mesh_terms: D045784:Abietanes / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D000072278:Forkhead Box Protein M1 / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D045784:Abietanes; D000972:Antineoplastic Agents, Phytogenic; C107585:FOXM1 protein, human; D000072278:Forkhead Box Protein M1; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C021751:tanshinone