title: Long-Term Evaluation of Biliary Reflux After Experimental One-Anastomosis Gastric Bypass in Rats.
abstract: Controversy remains regarding biliary reflux after one-anastomosis gastric bypass (OAGB). The aim of this "pilot" study was to analyze biliary reflux and its potential long-term consequences on esogastric mucosae in OAGB-operated rats.
  
  
  
  Diet-induced obese rats were subjected to OAGB (n = 10) or sham (n = 4) surgery and followed up for 16 weeks. Evolution of weight and glucose tolerance was analyzed. Bile acid concentration measurement, histological and qRT-PCR analysis were performed in the esogastric segments.
  
  
  
  Weight loss and glucose tolerance were improved after OAGB. Mean bile acid concentration was 4.2 times higher in the esogastric segments of OAGB rats (compared to sham). A foveolar hyperplasia of the gastro-jejunal anastomosis and an eosinophilic polynuclear cell infiltration were observed in OAGB rats. An esophageal hyper-papillomatosis was observed in both groups (OAGB = 50%, sham = 50%). qRT-PCR analysis showed no differences between OAGB and sham mRNA levels of Barrett's esophagus or esogastric carcinogenic-specific genes. No intestinal metaplasia, dysplasia, or cancer were observed after a 16-week follow-up.
  
  
  
  After a 16-week follow-up, this pilot study confirmed the good reproducibility of our OAGB rat model. OAGB rats had not developed any pre-cancerous or cancerous lesions. Further experimental studies with longer term follow-up are required.
authors.affiliation: Inserm UMR 1149, UFR de Médecine Paris Diderot, Université Paris Diderot, Sorbonne Paris Cité, DHU Unity APHP, 75890, Paris, France. matthieu.bruzzi@aphp.fr.|Service de Chirurgie Digestive, AP-HP Hôpital Européen Georges Pompidou, 75015, Paris, France. matthieu.bruzzi@aphp.fr.|ANCRE, EA 4465, Université Paris Descartes, Paris, France. matthieu.bruzzi@aphp.fr.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Bruzzi
authors.affiliation: Inserm UMR 1149, UFR de Médecine Paris Diderot, Université Paris Diderot, Sorbonne Paris Cité, DHU Unity APHP, 75890, Paris, France.
authors.forename: Henri
authors.initials: H
authors.lastname: Duboc
authors.affiliation: Inserm UMR 1149, UFR de Médecine Paris Diderot, Université Paris Diderot, Sorbonne Paris Cité, DHU Unity APHP, 75890, Paris, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Gronnier
authors.affiliation: Inserm UMR 7203, ERL U1057, Université Paris VI, Paris, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Rainteau
authors.affiliation: Inserm UMR 1149, UFR de Médecine Paris Diderot, Université Paris Diderot, Sorbonne Paris Cité, DHU Unity APHP, 75890, Paris, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Couvelard
authors.affiliation: Inserm UMR 1149, UFR de Médecine Paris Diderot, Université Paris Diderot, Sorbonne Paris Cité, DHU Unity APHP, 75890, Paris, France.
authors.forename: Maude
authors.initials: M
authors.lastname: Le Gall
authors.affiliation: Inserm UMR 1149, UFR de Médecine Paris Diderot, Université Paris Diderot, Sorbonne Paris Cité, DHU Unity APHP, 75890, Paris, France.
authors.forename: André
authors.initials: A
authors.lastname: Bado
authors.affiliation: Service de Chirurgie Digestive, AP-HP Hôpital Européen Georges Pompidou, 75015, Paris, France.|ANCRE, EA 4465, Université Paris Descartes, Paris, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Chevallier
mesh_terms: D000818:Animals; D001471:Barrett Esophagus / Q000209:etiology; D001647:Bile Acids and Salts / Q000032:analysis; D001655:Bile Reflux / Q000209:etiology*; D002299:Cardia / Q000473:pathology; D004947:Esophagus / Q000473:pathology; D015390:Gastric Bypass / Q000009:adverse effects* / Q000379:methods; D005951:Glucose Tolerance Test; D008297:Male; D008679:Metaplasia; D009767:Obesity, Morbid / Q000601:surgery*; D010865:Pilot Projects; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D015431:Weight Loss
chemical_list: D001647:Bile Acids and Salts
keywords: Biliary reflux; Mini-gastric bypass; Morbid obesity; One-anastomosis gastric bypass