title: A Cysteine-Specific Fluorescent Switch for Monitoring Oxidative Stress and Quantification of Aminoacylase-1 in Blood Serum.
abstract: Reagents that allows detection and monitoring of crucial biomarkers with luminescence ON response have significance in clinical diagnostics. A new coumarin derivative is reported here, which could be used for specific and efficient chemodosimetric detection of cysteine, an important biomarker. The probe is successfully used for studying the biochemical transformation of N-acetylcysteine, a commonly prescribed Cys supplement drug to Cys by aminoacylase-1 (ACY-1), an important and endogenous mammalian enzyme. The possibility of using this reagent for quantification of ACY-1 in blood serum samples is also explored. Nontoxic nature and cell membrane permeability are key features of this probe and are ideally suited for imaging intracellular Cys in normal and cancerous cell lines. Our studies have also revealed that this reagent could be utilized as a redox switch to monitor the hydrogen-peroxide-induced oxidative stress in living SW480 cell lines. Peroxide-mediated cysteine oxidation has a special significance for understanding the cellular-signaling events.
authors.affiliation: Organic Chemistry Division, CSIR-National Chemical Laboratory , Pune 411008, India.
authors.forename: Anila H
authors.initials: AH
authors.lastname: A
authors.affiliation: Organic Chemistry Division, CSIR-National Chemical Laboratory , Pune 411008, India.
authors.forename: Firoj
authors.initials: F
authors.lastname: Ali
authors.affiliation: Organic Chemistry Division, CSIR-National Chemical Laboratory , Pune 411008, India.
authors.forename: Shilpi
authors.initials: S
authors.lastname: Kushwaha
authors.affiliation: Chromatin and Disease Laboratory, National Center for Cell Science , Pune 411007, India.
authors.forename: Nandaraj
authors.initials: N
authors.lastname: Taye
authors.affiliation: Chromatin and Disease Laboratory, National Center for Cell Science , Pune 411007, India.|CSIR-Indian Institute of Chemical Biology , Kolkata 700032, India.
authors.forename: Samit
authors.initials: S
authors.lastname: Chattopadhyay
authors.affiliation: Organic Chemistry Division, CSIR-National Chemical Laboratory , Pune 411008, India.|CSIR-Central Salt and Marine Chemicals Research Institute , Bhavnagar 364002, Gujrat, India.
authors.forename: Amitava
authors.identifier: 0000-0003-3666-1743
authors.initials: A
authors.lastname: Das
mesh_terms: D000581:Amidohydrolases / Q000032:analysis / Q000097:blood* / Q000378:metabolism; D045744:Cell Line, Tumor; D003374:Coumarins / Q000737:chemistry*; D003545:Cysteine / Q000032:analysis* / Q000378:metabolism; D057075:Enzyme Assays / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D061848:Optical Imaging / Q000379:methods; D018384:Oxidative Stress; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D003374:Coumarins; D005456:Fluorescent Dyes; C030123:coumarin; D006861:Hydrogen Peroxide; D000581:Amidohydrolases; C019672:aminoacylase I; D003545:Cysteine