title: Transformation products of clindamycin in moving bed biofilm reactor (MBBR).
abstract: Clindamycin is widely prescribed for its ability to treat a number of common bacterial infections. Thus, clindamycin enters wastewater via human excretion or disposal of unused medication and widespread detection of pharmaceuticals in rivers proves the insufficiency of conventional wastewater treatment plants in removing clindamycin. Recently, it has been discovered that attached biofilm reactors, e.g., moving bed biofilm reactors (MBBRs) obtain a higher removal of pharmaceuticals than conventional sludge wastewater treatment plants. Therefore, this study investigated the capability of MBBRs applied in the effluent of conventional wastewater treatment plants to remove clindamycin. First, a batch experiment was executed with a high initial concentration of clindamycin to identify the transformation products. It was shown that clindamycin can be removed from wastewater by MBBR and the treatment process converts clindamycin into the, possibly persistent, products clindamycin sulfoxide and N-desmethyl clindamycin as well as 3 other mono-oxygenated products. Subsequently, the removal kinetics of clindamycin and the formation of the two identified products were investigated in batch experiments using MBBR carriers from polishing and nitrifying reactors. Additionally, the presence of these two metabolites in biofilm-free wastewater effluent was studied. The nitrifying biofilm reactor had a higher biological activity with k-value of 0.1813 h-1than the reactor with polishing biofilm (k = 0.0161 h-1) which again has a much higher biological activity for removal of clindamycin than of the suspended bacteria (biofilm-free control). Clindamycin sulfoxide was the main transformation product which was found in concentrations exceeding 10% of the initial clindamycin concentration after 1 day of MBBR treatment. Thus, MBBRs should not necessarily be considered as reactors mineralizing clindamycin as they perform transformation reactions at least to some extent.
authors.affiliation: Department of Environmental Engineering, Technical University of Denmark, Bygningstorvet 115, 2800, Kgs. Lyngby, Denmark; Institute of Environmental Science, Aarhus University, Frederiksborgsvej 399, 4000, Roskilde, Denmark.
authors.forename: Gordon T H
authors.initials: GTH
authors.lastname: Ooi
authors.affiliation: Institute of Environmental Science, Aarhus University, Frederiksborgsvej 399, 4000, Roskilde, Denmark.
authors.forename: Monica
authors.initials: M
authors.lastname: Escola Casas
authors.affiliation: Department of Environmental Engineering, Technical University of Denmark, Bygningstorvet 115, 2800, Kgs. Lyngby, Denmark.
authors.forename: Henrik R
authors.initials: HR
authors.lastname: Andersen
authors.affiliation: Institute of Environmental Science, Aarhus University, Frederiksborgsvej 399, 4000, Roskilde, Denmark. Electronic address: kb@envs.au.dk.
authors.forename: Kai
authors.initials: K
authors.lastname: Bester
mesh_terms: D018441:Biofilms; D019149:Bioreactors / Q000382:microbiology*; D002981:Clindamycin; D012722:Sewage / Q000382:microbiology; D014865:Waste Disposal, Fluid; D062065:Wastewater
chemical_list: D002981:Clindamycin; D012722:Sewage; D062065:Wastewater
keywords: Antibiotics; Biofilm; Degradation; Metabolites; Waste water