title: IL23Rsingle nucleotide polymorphisms could be either beneficial or harmful in ulcerative colitis.
abstract: OBJECTIVE
  To investigate the association of seven single nucleotide polymorphisms (SNPs) of theIL23Rgene with the clinical picture of ulcerative colitis (UC).
  
  
  METHODS
  Genomic DNA samples of 131 patients (66 males, 65 females, mean age 55.4 ± 15.8 years) with Caucasian origin, diagnosed with UC were investigated. The diagnosis of UC was based on the established clinical, endoscopic, radiological, and histopathological guidelines. DNA was extracted from peripheral blood leukocytes by routine salting out method. Polymerase chain reaction and restriction fragment length polymorphism were used to identify the alleles of seven SNPs ofIL23Rgene (rs11209026, rs10889677, rs1004819, rs2201841, rs7517847, rs10489629, rs7530511).
  
  
  RESULTS
  Four out of seven analyzed SNPs had statistically significant influence on the clinical picture of UC. Two SNPs were associated with greater colonic extension (rs2201841P= 0.0084; rs10489629P= 0.0405). For two of the SNPs, there was more frequently need for operations (rs2201841P= 0.0348, OR = 8.0; rs10889677P= 0.0347, OR = 8.0). The rs2201841 showed to be a risk factor for the development of iron deficiency (P= 0.0388, OR = 6.1837). For patients with the rs10889677, a therapy with azathioprine was more frequently necessary (P= 0.0116, OR = 6.1707). Patients with rs10489629 SNP had a lower risk for weight loss (P= 0.0169, OR = 0.3394). Carriers of the heterozygous variant had a higher risk for an extended disease (P= 0.0284). The rs7517847 showed a protective character leading to mild bowel movements. Three SNPs demonstrated no statistically significant influence on any examined clinical features of UC.
  
  
  CONCLUSIONS
  We demonstrated susceptible or protective character of the investigatedIL23RSNPs on the phenotype of UC, confirming the genetic association.
authors.affiliation: Sarah Fischer, Patrícia Sarlós, Division of Gastroenterology, 1 Department of Internal Medicine, University of Pécs, 7624 Pécs, Hungary.
authors.forename: Sarah
authors.initials: S
authors.lastname: Fischer
authors.affiliation: Sarah Fischer, Patrícia Sarlós, Division of Gastroenterology, 1 Department of Internal Medicine, University of Pécs, 7624 Pécs, Hungary.
authors.forename: Erzsébet
authors.initials: E
authors.lastname: Kövesdi
authors.affiliation: Sarah Fischer, Patrícia Sarlós, Division of Gastroenterology, 1 Department of Internal Medicine, University of Pécs, 7624 Pécs, Hungary.
authors.forename: Lili
authors.initials: L
authors.lastname: Magyari
authors.affiliation: Sarah Fischer, Patrícia Sarlós, Division of Gastroenterology, 1 Department of Internal Medicine, University of Pécs, 7624 Pécs, Hungary.
authors.forename: Veronika
authors.initials: V
authors.lastname: Csöngei
authors.affiliation: Sarah Fischer, Patrícia Sarlós, Division of Gastroenterology, 1 Department of Internal Medicine, University of Pécs, 7624 Pécs, Hungary.
authors.forename: Kinga
authors.initials: K
authors.lastname: Hadzsiev
authors.affiliation: Sarah Fischer, Patrícia Sarlós, Division of Gastroenterology, 1 Department of Internal Medicine, University of Pécs, 7624 Pécs, Hungary.
authors.forename: Béla
authors.initials: B
authors.lastname: Melegh
authors.affiliation: Sarah Fischer, Patrícia Sarlós, Division of Gastroenterology, 1 Department of Internal Medicine, University of Pécs, 7624 Pécs, Hungary.
authors.forename: Péter
authors.initials: P
authors.lastname: Hegyi
authors.affiliation: Sarah Fischer, Patrícia Sarlós, Division of Gastroenterology, 1 Department of Internal Medicine, University of Pécs, 7624 Pécs, Hungary.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Sarlós
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D018798:Anemia, Iron-Deficiency / Q000235:genetics; D001379:Azathioprine / Q000627:therapeutic use; D003093:Colitis, Ulcerative / Q000150:complications / Q000188:drug therapy / Q000235:genetics*; D003106:Colon / Q000503:physiopathology; D005260:Female; D056726:Genetic Association Studies; D006579:Heterozygote; D006801:Humans; D006814:Hungary; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D018123:Receptors, Interleukin / Q000235:genetics*; D012307:Risk Factors; D015431:Weight Loss / Q000235:genetics; D044465:White People
chemical_list: C515500:IL23R protein, human; D007166:Immunosuppressive Agents; D018123:Receptors, Interleukin; D001379:Azathioprine
keywords: Hungarian; IL23R gene; phenotype; polymorphism; ulcerative colitis