title: Determination of urazamide in pharmaceutical preparation with room temperature ionic liquid.
abstract: A high performance liquid chromatographic method was developed and validated for the determination of urazamide in pharmaceutical preparation with novel green aqueous mobile phase modified with room temperature ionic liquids (RTILs). 1-Ethyl-3-methyl-imidazolium tetrafluoroborate ([EMIM][BF4]) was selected as a mobile phase additive to improve retention and avoid baseline disturbances at t0. Various mobile phase parameters such as cation moiety, chaotropic anion moiety, pH and concentration of RTILs were optimized to determine urazamide at the proper retention time. The assay was validated according to International Conference on Harmonization guidelines. The linearity of the calibration curve was good (r2> 0.999). Intra-day precision varied between 0.50 and 1.23%. Relative standard deviations of inter-day precision ranged between 1.07 and 1.66%. Recoveries in tablets ranged between 99.7 and 101.2% and it was successfully applied to determine urazamide in pharmaceutical preparations.
authors.affiliation: College of Pharmacy, Kangwon National University, Chuncheon, 24341, Korea.
authors.forename: Narae
authors.initials: N
authors.lastname: Seo
authors.affiliation: College of Pharmacy, Kangwon National University, Chuncheon, 24341, Korea.
authors.forename: Yoo Rim
authors.initials: YR
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Kangwon National University, Chuncheon, 24341, Korea.
authors.forename: Hyun Sun
authors.initials: HS
authors.lastname: Park
authors.affiliation: College of Pharmacy, Kangwon National University, Chuncheon, 24341, Korea.
authors.forename: Quoc-Ky
authors.initials: QK
authors.lastname: Truong
authors.affiliation: College of Pharmacy, Kangwon National University, Chuncheon, 24341, Korea.
authors.forename: Jae-Yong
authors.initials: JY
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Kangwon National University, Chuncheon, 24341, Korea.
authors.forename: Hyun Kyu
authors.initials: HK
authors.lastname: Chung
authors.affiliation: College of Pharmacy, Kangwon National University, Chuncheon, 24341, Korea.
authors.forename: Yusung
authors.initials: Y
authors.lastname: Choi
authors.affiliation: College of Pharmacy, Chung-Ang University, Seoul, 06974, Korea.
authors.forename: Byeonghee
authors.initials: B
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Chung-Ang University, Seoul, 06974, Korea.
authors.forename: Sang Beom
authors.initials: SB
authors.lastname: Han
authors.affiliation: College of Pharmacy, Kangwon National University, Chuncheon, 24341, Korea. kyeong@kangwon.ac.kr.
authors.forename: Kyeong Ho
authors.identifier: http://orcid.org/0000-0002-6017-2256
authors.initials: KH
authors.lastname: Kim
mesh_terms: D000620:Aminoimidazole Carboxamide / Q000737:chemistry*; D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles; D007202:Indicators and Reagents; D052578:Ionic Liquids / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000032:analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets / Q000032:analysis
chemical_list: C499119:1-ethyl-3-methylimidazolium tetrafluoroborate; D007093:Imidazoles; D007202:Indicators and Reagents; D052578:Ionic Liquids; D004364:Pharmaceutical Preparations; D013607:Tablets; D001224:Aspartic Acid; D000620:Aminoimidazole Carboxamide; C006893:ureidosuccinic acid
keywords: Reverse phase high performance liquid chromatography (RP-HPLC); Room temperature ionic liquids (RTILs); Urazamide; Very polar compound