title: The Role of Ischemia Modified Albumin and D-dimer as Early or Late Biochemical Markers in Ovarian Torsion.
abstract: OBJECTIVE
  Our aim was to examine the potential roles of ischemia modified albumin(IMA) and D-dimer as reliable early diagnostic markers of ovarian torsion.
  
  
  METHODS
  24 Wistar albino rats were included and randomized into three groups. Control (sham) rats underwent laparotomy then bilateral ovaries removed for histopathological examination and concomitant blood sampling for IMA and D-dimer assays after 4 h. In the remaining groups, ovarian ischemia was achieved by rotating bilateral ovaries 1080° clockwise then blood samples were obtained and ovaries were removed after 4 h and 24 h. IMA and D-dimer levels as well as the histopathological injury scores were assessed in all groups.
  
  
  RESULTS
  A statistically higher significant difference in D-dimer levels in the 4-h torsion group (Group 2) than the other groups (P = 0.001, P < 0.01). Despite absence of statistical significance, the difference in IMA levels between the groups was close to statistical significance (P = 0.064; P > 0.05); accordingly IMA levels were higher in the 24-h torsion group (Group 3), than in 4-h torsion (Group 2) or sham (Group 1) group.
  
  
  CONCLUSIONS
  Our results suggest that D-dimer may represent a valuable acute marker of ovarian torsion. The cut-off value for D-dimer was 402 ng/ml. Although rats in Group3 had higher IMA than in other groups, the difference was only close to statistical significance. Also, increasing duration of torsion was associated with reduced D-dimer levels, while IMA levels exhibited an increase during the 24 h period. In contrast with previous studies, IMA appeared to be a potential marker in the long term rather than the short term.
authors.affiliation: Bafra State Hospital Gynecology and Obstetric.
authors.forename: Ayca
authors.initials: A
authors.lastname: Karatas Gurgun
authors.affiliation: İstanbul Training and Research Hospital Gynecology and Obstetric.
authors.forename: Isık
authors.initials: I
authors.lastname: Kaban
authors.affiliation: Haydarpasa Numune Training And Research Hospital Gynecology and Obstetric.
authors.forename: Nurettin
authors.initials: N
authors.lastname: Aka
authors.affiliation: Karadeniz Technical University Biochemistry.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Mentese
authors.affiliation: Haydarpasa Numune Training and Research Hospital Patology.
authors.forename: Fugen
authors.initials: F
authors.lastname: Aker
authors.affiliation: Haydarpasa Numune Training and Research Hospital Biochemistry.
authors.forename: Ahmet Rıza
authors.initials: AR
authors.lastname: Uras
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D004195:Disease Models, Animal; D005260:Female; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis*; D007511:Ischemia / Q000097:blood*; D010049:Ovarian Diseases / Q000097:blood*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D000075462:Serum Albumin, Human; D014102:Torsion Abnormality / Q000097:blood*
chemical_list: D015415:Biomarkers; D005338:Fibrin Fibrinogen Degradation Products; C036309:fibrin fragment D; C561522:ischemia-modified albumin; D000075462:Serum Albumin, Human
keywords: D-dimer; IMA; ovarian torsion modal