title: Phytochelatin 2 accumulates in roots of the seagrass Enhalus acoroides collected from sediment highly contaminated with lead.
abstract: Phytochelatins (PCs), the heavy metal-binding peptides of plants, play a main function in heavy metal detoxification. In this study, Enhalus acoroides samples collected at six distinct seagrass beds from the coast of Khanh Hoa province, Viet Nam, were evaluated for their PCs. The contents of different PCs in each organ including leaf, rhizome, and root were determined by using HPLC analysis. Significant differences of PC2contents among specific organs and their relation were tested by ANOVA, Tukey test, and Pearson's correlation. The results showed that higher PC2, appearance of PC3and a strong correlation between PC2and Pb concentration were found in the root organ collected from a Pb contaminated area. We conclude that high Pb in the sediment induce high PC2and PC3production in the root. This first report on in situ detection of PCs of seagrass encourages future investigation on the ability to use seagrass for phytoremediation and as a bioindicator of heavy metals based on PC contents.
authors.affiliation: Departement of Marine Botany, Institute of Oceanography, Vietnam Academy of Science and Technology, 01 Cau Da, Nha Trang City, Vietnam. nguyenxuanvi@gmail.com.
authors.forename: Xuan-Vy
authors.initials: XV
authors.lastname: Nguyen
authors.affiliation: Department of Marine Biochemistry, Institute of Oceanography, Vietnam Academy of Science and Technology, Nha Trang City, Vietnam.
authors.forename: Khanh-Hy
authors.initials: KH
authors.lastname: Le-Ho
authors.affiliation: Institute of Botany, Leibniz University-Hannover, Hannover, Germany.
authors.forename: Jutta
authors.initials: J
authors.lastname: Papenbrock
mesh_terms: D059001:Aquatic Organisms; D001673:Biodegradation, Environmental; D002614:Chelating Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D019015:Geologic Sediments / Q000737:chemistry; D031321:Hydrocharitaceae / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D007854:Lead / Q000302:isolation & purification*; D054811:Phytochelatins / Q000096:biosynthesis* / Q000138:chemical synthesis / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D027343:Rhizome / Q000737:chemistry / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*
chemical_list: D002614:Chelating Agents; D010936:Plant Extracts; D014874:Water Pollutants, Chemical; D007854:Lead; D054811:Phytochelatins
keywords: Correlation; Enhalus acoroides; Lead; Phytochelatins; Seagrasses