title: Magnesium Lithospermate B from Salvia miltiorrhiza Bunge Ameliorates Aging-Induced Renal Inflammation and Senescence via NADPH Oxidase-Mediated Reactive Oxygen Generation.
abstract: The present study was conducted to examine whether magnesium lithospermate B (MLB) extracted from Salviae miltiorrhizae radix was renoprotective in pathways related to age-related oxidative stress in aged rats. Magnesium lithospermate B was orally administered at a dose of 2- or 8-mg/kg body weight for 16 consecutive days, and the effects were compared with those of vehicle in old and young rats. Magnesium lithospermate B administration to old rats ameliorated renal oxidative stress through reduction of reactive oxygen species. The old rats exhibited a dysregulation of the expression of proteins related to oxidative stress and inflammation in the kidneys, and MLB administration significantly reduced the protein expression of major subunits of nicotinamide adenine dinucleotide phosphate oxidase (Nox4 and p22phox), phospho-p38, nuclear factor-kappa B p65, cyclooxygenase-2, and inducible nitric oxide synthase. In addition, MLB-treated old rats showed lower levels of senescence-related proteins such as p16, ADP-ribosylation factor 6, p53, and p21 through effects on the mitogen-activated protein kinase pathway. Magnesium lithospermate B administration also significantly attenuated the age-related increase in serum urea nitrogen, reflecting renal dysfunction, up-regulated podocyte structural proteins, and reduced renal structural injury. Our results provide important evidence that MLB reduces the renal damage of oxidative stress in old rats. Copyright © 2017 John Wiley & Sons, Ltd.
authors.affiliation: Department of Medicinal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong, 369-873, Korea.|Molecular Inflammation Research Center for Aging Intervention (MRCA), College of Pharmacy, Pusan National University, Busan, 609-735, Korea.|College of Korean Medicine, Daegu Haany University, Daegu, 706-060, Korea.
authors.forename: Chan Hum
authors.initials: CH
authors.lastname: Park
authors.affiliation: Animal and Plant Quarantine Agency, Gyeongsangbuk-do, 39660, Korea.|College of Korean Medicine, Daegu Haany University, Daegu, 706-060, Korea.
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Shin
authors.affiliation: Molecular Inflammation Research Center for Aging Intervention (MRCA), College of Pharmacy, Pusan National University, Busan, 609-735, Korea.
authors.forename: Eun Kyeong
authors.initials: EK
authors.lastname: Lee
authors.affiliation: Molecular Inflammation Research Center for Aging Intervention (MRCA), College of Pharmacy, Pusan National University, Busan, 609-735, Korea.
authors.forename: Dae Hyun
authors.identifier: http://orcid.org/0000-0003-2423-1973
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Molecular Inflammation Research Center for Aging Intervention (MRCA), College of Pharmacy, Pusan National University, Busan, 609-735, Korea.
authors.forename: Min-Jo
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: College of Korean Medicine, Daegu Haany University, Daegu, 706-060, Korea.
authors.forename: Seong-Soo
authors.initials: SS
authors.lastname: Roh
authors.affiliation: Graduate School of Science and Engineering for Research, University of Toyama, Toyama, 930-8555, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Yokozawa
authors.affiliation: Molecular Inflammation Research Center for Aging Intervention (MRCA), College of Pharmacy, Pusan National University, Busan, 609-735, Korea.
authors.forename: Hae Young
authors.identifier: http://orcid.org/0000-0002-3215-8828
authors.initials: HY
authors.lastname: Chung
mesh_terms: D000089982:ADP-Ribosylation Factor 6; D020727:ADP-Ribosylation Factors; D000375:Aging / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D007249:Inflammation / Q000378:metabolism; D007674:Kidney Diseases / Q000188:drug therapy*; D008297:Male; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D019255:NADPH Oxidases / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D027561:Salvia miltiorrhiza / Q000737:chemistry*
chemical_list: D000089982:ADP-Ribosylation Factor 6; D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; D017382:Reactive Oxygen Species; C061091:lithospermate B; D051546:Cyclooxygenase 2; D019255:NADPH Oxidases; C022709:superoxide-forming enzyme; D020928:Mitogen-Activated Protein Kinases; D020727:ADP-Ribosylation Factors; C000715349:Arf6 protein, rat
keywords: aging; inflammation; magnesium lithospermate B; oxidative stress; renal damage; senescence