title: Evaluation of TNF-alpha gene (G308A) and MBL2 gene codon 54 polymorphisms in Turkish patients with tuberculosis.
abstract: OBJECTIVE
  MBL acts as a binding protein that enables uptake of mycobacteria into macrophages. And, TNF-alpha is an important cytokine that is involved in control of mycobacterial infections both in-vivo and in-vitro. A large number of genetic factors exerting susceptibility to tuberculosis has been identified, among which mannose-binding lectin and tumor necrosis factor-alpha call attention. The objective of this study is to compare the frequency of TNF-alpha and MBL gene polymorphisms between patients diagnosed with tuberculosis and healthy volunteers in Turkey, and determine the association between tuberculosis and TNF-alpha gene (G308A) and MBL2 gene codon 54 polymorphisms.
  
  
  METHODS
  The study included 69 patients who were diagnosed with tuberculosis and 70 control subjects. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to detect TNF-alpha (G308A) gene and MBL2 gene codon 54 polymorphisms. For statistical analysis, the significance level was determined as p<0.05.
  
  
  RESULTS
  A comparison between patient and control groups in TNF-alpha (G308A) gene and MBL2 gene codon 54 polymorphisms showed no statistically significant difference (p>0.05). However, a comparison of mean body mass index (BMI) and smoking status showed a statistically significant difference between the tuberculosis and control groups (p=0.01 and p=0.009, respectively).
  
  
  CONCLUSIONS
  Our results suggest that the MBL2 gene Codon 54 and TNF-alpha gene G308A polymorphisms are not associated with an increased risk for development of tuberculosis in our patients. Further studies are required including more cases of tuberculosis patients and other potentially relevant gene polymorphisms.
authors.affiliation: Malatya Training and Research Hospital, Department of Chest Disease, Malatya, Turkey.
authors.forename: Esma
authors.initials: E
authors.lastname: Ceylan
authors.affiliation: Department of Medical Genetics, Sakarya University Training and Research Hospital, Sakarya, Turkey.
authors.forename: Mutlu
authors.initials: M
authors.lastname: Karkucak
authors.affiliation: Chest Disease, Sakarya University Training and Research Hospital, Sakarya 54100, Turkey. Electronic address: hikmetcoban04@gmail.com.
authors.forename: Hikmet
authors.initials: H
authors.lastname: Coban
authors.affiliation: Department of Chest Disease, Uludag University Faculty of Medicine, Bursa, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Karadag
authors.affiliation: Department of Medical Genetics, Uludag University Faculty of Medicine, Bursa, Turkey.
authors.forename: Tahsin
authors.initials: T
authors.lastname: Yakut
mesh_terms: D000328:Adult; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D060005:Genotyping Techniques; D006801:Humans; D008297:Male; D037601:Mannose-Binding Lectin / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D014376:Tuberculosis / Q000235:genetics* / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D014421:Turkey
chemical_list: C445619:MBL2 protein, human; D037601:Mannose-Binding Lectin; D014409:Tumor Necrosis Factor-alpha
keywords: Gene polymorphism; Mannose-binding lectin; Tuberculosis; Tumor necrosis factor-alpha