title: Expression profiling of TRIM protein family in THP1-derived macrophages following TLR stimulation.
abstract: Activated macrophages play an important role in many inflammatory diseases including septic shock and atherosclerosis. However, the molecular mechanisms limiting macrophage activation are not completely understood. Members of the tripartite motif (TRIM) family have recently emerged as important players in innate immunity and antivirus. Here, we systematically analyzed mRNA expressions of representative TRIM molecules in human THP1-derived macrophages activated by different toll-like receptor (TLR) ligands. Twenty-nine TRIM members were highly induced (>3 fold) by one or more TLR ligands, among which 19 of them belong to TRIM C-IV subgroup. Besides TRIM21, TRIM22 and TRIM38 were shown to be upregulated by TLR3 and TLR4 ligands as previous reported, we identified a novel group of TRIM genes (TRIM14, 15, 31, 34, 43, 48, 49, 51 and 61) that were significantly up-regulated by TLR3 and TLR4 ligands. In contrast, the expression of TRIM59 was down-regulated by TLR3 and TLR4 ligands in both human and mouse macrophages. The alternations of the TRIM proteins were confirmed by Western blot. Finally, overexpression of TRIM59 significantly suppressed LPS-induced macrophage activation, whereas siRNA-mediated knockdown of TRIM59 enhanced LPS-induced macrophage activation. Taken together, the study provided an insight into the TLR ligands-induced expressions of TRIM family in macrophages.
authors.affiliation: Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi, 330031, China.
authors.forename: Mei-Xiu
authors.initials: MX
authors.lastname: Jiang
authors.affiliation: Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi, 330031, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Hong
authors.affiliation: Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi, 330031, China.
authors.forename: Bin-Bin
authors.initials: BB
authors.lastname: Liao
authors.affiliation: Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi, 330031, China.
authors.forename: Shui-Zhen
authors.initials: SZ
authors.lastname: Shi
authors.affiliation: Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi, 330031, China.
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Lai
authors.affiliation: Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi, 330031, China.
authors.forename: Huai-Yu
authors.initials: HY
authors.lastname: Zheng
authors.affiliation: Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi, 330031, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xie
authors.affiliation: Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi, 330031, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi, 330031, China.
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Wang
authors.affiliation: Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi, 330031, China.
authors.forename: Hong-Bo
authors.initials: HB
authors.lastname: Xin
authors.affiliation: Department of Basic Medical Science, Shock/Trauma Research Center, School of Medicine, University of Missouri Kansas City, Kansas City, MO, 64108, USA.
authors.forename: Mingui
authors.initials: M
authors.lastname: Fu
authors.affiliation: Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi, 330031, China.
authors.forename: Ke-Yu
authors.initials: KY
authors.lastname: Deng
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D006801:Humans; D007113:Immunity, Innate; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology*; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D051193:Toll-Like Receptors / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D008070:Lipopolysaccharides; D008565:Membrane Proteins; C114030:TRAT1 protein, human; C510344:TRIM protein, mouse; D051193:Toll-Like Receptors