title: Hyaluronidase-inhibitory activities of glycosaminoglycans from Liparis tessellatus eggs.
abstract: Polysaccharide fractions isolated from L. tessellatus eggs were purified and eluted using the DEAE-sepharose fast flow column. These were collected, tested and pooled based on their sugars content: F1, F2, and F3 which contain 26.8, 23.3, and 20.2% sulfated glycans; 34.5, 38.2, and 45.0% uronic acids; and 23.5, 19.0, and 7.5% acetylhexosamines and hexosamines, respectively. Hyaluronidase inhibitory effects of the fractions are in the order F3>F2>F1>Ascorbic acid, with F3 having the highest inhibition among the fractions and that of the standard, ascorbic acid. The electrospray ionization tandem mass spectrometry (ESI-MS/MS) confirmed the presence of uronic acids on F3, which could be a0,2A2fragment plus loss of methyl group which is very common among non-methylated, sulfated disaccharides.
authors.affiliation: Department of Seafood and Aquaculture Science, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-gil, Tongyeong, Gyeongnam, 650-160, South Korea; College of Arts and Sciences, Iloilo Science and Technology University, Burgos St., La Paz, Iloilo City, 5000, Philippines. Electronic address: berna2423@gmail.com.
authors.forename: Bernadeth F
authors.initials: BF
authors.lastname: Ticar
authors.affiliation: Department of Seafood and Aquaculture Science, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-gil, Tongyeong, Gyeongnam, 650-160, South Korea; Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.
authors.forename: Zuliyati
authors.initials: Z
authors.lastname: Rohmah
authors.affiliation: Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, Building 220, 2800, Kongens Lyngby, Denmark.
authors.forename: Solange I
authors.initials: SI
authors.lastname: Mussatto
authors.affiliation: Department of Chemistry, Changwon National University, Changwon, 53210, South Korea.
authors.forename: Jae-Min
authors.initials: JM
authors.lastname: Lim
authors.affiliation: Department of Chemistry, Changwon National University, Changwon, 53210, South Korea.
authors.forename: Seongha
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Seafood and Aquaculture Science, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-gil, Tongyeong, Gyeongnam, 650-160, South Korea.
authors.forename: Byeong-Dae
authors.initials: BD
authors.lastname: Choi
mesh_terms: D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects; D006025:Glycosaminoglycans / Q000302:isolation & purification / Q000494:pharmacology*; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors*; D010063:Ovum / Q000737:chemistry*; D010473:Perciformes; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D006025:Glycosaminoglycans; D006821:Hyaluronoglucosaminidase
keywords: Hyaluronic acid; Hyaluronidase inhibitory effect; L. tessellatus eggs