title: Harvesting pre-polarized macrophages using thermo-responsive substrates.
abstract: In the cell culture environment macrophages are highly adherent cells. Currently used methods to harvest macrophages have the disadvantage of reducing cell viability and their ability to re-attach after seeding. Although thermo-responsive surfaces have been employed to harvest cell sheets no reports are available to use these to harvest (pre-polarized) macrophages. We show that this method significantly improves the yield of living macrophages and percentage of subsequent cell reattachment, whilst having a minimal effect on the cell phenotype.
authors.affiliation: Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstr. 5, CH-9014 St. Gallen, Switzerland.
authors.forename: Vera
authors.initials: V
authors.lastname: Malheiro
authors.affiliation: Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstr. 5, CH-9014 St. Gallen, Switzerland.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Elbs-Glatz
authors.affiliation: Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstr. 5, CH-9014 St. Gallen, Switzerland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Obarzanek-Fojt
authors.affiliation: Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstr. 5, CH-9014 St. Gallen, Switzerland.
authors.forename: Katharina
authors.initials: K
authors.lastname: Maniura-Weber
authors.affiliation: Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstr. 5, CH-9014 St. Gallen, Switzerland.
authors.forename: Arie
authors.initials: A
authors.lastname: Bruinink