title: Simplified, rapid, and inexpensive estimation of water primary productivity based on chlorophyll fluorescence parameter Fo.
abstract: Primary productivity in water environment relies on the photosynthetic production of microalgae. Chlorophyll fluorescence is widely used to detect the growth status and photosynthetic efficiency of microalgae. In this study, a method was established to determine the Chl a content, cell density of microalgae, and water primary productivity by measuring chlorophyll fluorescence parameter Fo. A significant linear relationship between chlorophyll fluorescence parameter Fo and Chl a content of microalgae, as well as between Fo and cell density, was observed under pure-culture conditions. Furthermore, water samples collected from natural aquaculture ponds were used to validate the correlation between Fo and water primary productivity, which is closely related to Chl a content in water. Thus, for a given pure culture of microalgae or phytoplankton (mainly microalgae) in aquaculture ponds or other natural ponds for which the relationship between the Fo value and Chl a content or cell density could be established, Chl a content or cell density could be determined by measuring the Fo value, thereby making it possible to calculate the water primary productivity. It is believed that this method can provide a convenient way of efficiently estimating the primary productivity in natural aquaculture ponds and bringing economic value in limnetic ecology assessment, as well as in algal bloom monitoring.
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, China. Electronic address: chenhui@ihb.ac.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, China; University of Chinese Academy of Sciences, Beijing, China. Electronic address: weizhou426@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, China. Electronic address: chenweixian@ihb.ac.cn.
authors.forename: Weixian
authors.initials: W
authors.lastname: Chen
authors.affiliation: TONGWEI COMPANY LIMITED, Chengdu, Sichuang, China. Electronic address: xiew@tongwei.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Xie
authors.affiliation: TONGWEI COMPANY LIMITED, Chengdu, Sichuang, China. Electronic address: jianglp@tongwei.com.
authors.forename: Liping
authors.initials: L
authors.lastname: Jiang
authors.affiliation: TONGWEI COMPANY LIMITED, Chengdu, Sichuang, China. Electronic address: liangql@tongwei.com.
authors.forename: Qinlang
authors.initials: Q
authors.lastname: Liang
authors.affiliation: TONGWEI COMPANY LIMITED, Chengdu, Sichuang, China. Electronic address: huangmj01@tongwei.com.
authors.forename: Mingjun
authors.initials: M
authors.lastname: Huang
authors.affiliation: TONGWEI COMPANY LIMITED, Chengdu, Sichuang, China. Electronic address: wuzw@tongwei.com.
authors.forename: Zongwen
authors.initials: Z
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, China. Electronic address: wangqiang@ihb.ac.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
mesh_terms: D002452:Cell Count; D002681:China; D002734:Chlorophyll / Q000378:metabolism*; D002947:Cities; D005453:Fluorescence; D058086:Microalgae / Q000166:cytology / Q000502:physiology; D010788:Photosynthesis; D010827:Physiology / Q000191:economics* / Q000379:methods*; D015203:Reproducibility of Results; D014867:Water / Q000378:metabolism*
chemical_list: D014867:Water; D002734:Chlorophyll
keywords: Cell density; Chl a content; Chlorophyll fluorescence; Microalgae; Primary productivity