title: Recruitment of PP1 to the centrosomal scaffold protein CEP192.
abstract: Centrosomal protein of 192 kDa (CEP192) is a scaffolding protein that recruits the mitotic protein kinases Aurora A and PLK1 to the centrosome. Here we demonstrate that CEP192 also recruits the type one protein phosphatase (PP1) via a highly conserved KHVTF docking motif. The threonine of the KHVTF motif is phosphorylated during mitosis and protein kinase inhibition studies suggest this to be a PLK1-dependent process.
authors.affiliation: Department of Biological Sciences, University of Calgary, Calgary, Alberta T2N 1N4, Canada.
authors.forename: Isha
authors.initials: I
authors.lastname: Nasa
authors.affiliation: Department of Cellular & Molecular Biology and Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, ON, Canada.
authors.forename: Laura
authors.initials: L
authors.lastname: Trinkle-Mulcahy
authors.affiliation: Departments of Biochemistry & Molecular Biology and Oncology, Robson DNA Science Centre, Southern Alberta Cancer Research Institute, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: P
authors.initials: P
authors.lastname: Douglas
authors.affiliation: Department of Biological Sciences, University of Calgary, Calgary, Alberta T2N 1N4, Canada.
authors.forename: Sibapriya
authors.initials: S
authors.lastname: Chaudhuri
authors.affiliation: Departments of Biochemistry & Molecular Biology and Oncology, Robson DNA Science Centre, Southern Alberta Cancer Research Institute, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: S P
authors.initials: SP
authors.lastname: Lees-Miller
authors.affiliation: Laboratory of Metabolism, National Cancer Institute, Bethesda, MD 20892, USA.
authors.forename: Kyung S
authors.initials: KS
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences, University of Calgary, Calgary, Alberta T2N 1N4, Canada. Electronic address: moorhead@ucalgary.ca.
authors.forename: Greg B
authors.initials: GB
authors.lastname: Moorhead
mesh_terms: D001665:Binding Sites; D018385:Centrosome / Q000378:metabolism* / Q000648:ultrastructure; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry* / Q000378:metabolism*; D004789:Enzyme Activation; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis / Q000502:physiology*; D010766:Phosphorylation; D011485:Protein Binding; D017476:Receptors, Neuropeptide Y / Q000737:chemistry* / Q000378:metabolism*
chemical_list: C526596:Cep192 protein, human; D002868:Chromosomal Proteins, Non-Histone; D017476:Receptors, Neuropeptide Y; C109491:neuropeptide Y4 receptor
keywords: CEP192; Centrosome; Mitosis; Polo-like kinase one; Protein phosphatase one