title: Viral hemorrhagic septicemia virus glycoprotein production in tobacco.
abstract: Viral hemorrhagic septicemia virus (VHSV) causes mortality in numerous marine and freshwater fish species resulting in heavy losses in fish farming. The glycoprotein gene of VHSV was fused with the cholera toxin B subunit (CTB) and expressed transiently in leaf tissues of Nicotiana benthamiana via the agroinfiltration method. The glycoprotein gene was divided into two parts to improve assembly of CTB fusion proteins (CTB-VHSV99-235and CTB-VHSV258-417). Production of CTB fusion proteins was confirmed in the agroinfiltrated leaf tissue by western blot analysis. The plant-produced CTB fusion proteins showed biological activity to GM1-ganglioside, a receptor for biologically active CTB, on GM1-ELISA. The expression level of the CTB-VHSV fusion proteins was 0.86% (CTB-VHSV99-235) and 0.93% (CTB-VHSV258-417) of total proteins in agroinfiltrated leaf tissue, as determined by GM1-ELISA. These results suggest that Agrobacterium-mediated transient expression of CTB fusion antigens of VHSV is a rapid and convenient method and demonstrate the feasibility of using agroinfiltrated plant leaf tissues expressing CTB-fusion antigens as a plant-based vaccine to prevent VHSV infection.
authors.affiliation: Department of Bioactive Material Sciences, Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeollabuk-do 54896, Republic of Korea.
authors.forename: Nguyen-Quang-Duc
authors.initials: NQ
authors.lastname: Tien
authors.affiliation: College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju, 61186, Republic of Korea.
authors.forename: Tae-Jung
authors.initials: TJ
authors.lastname: Kim
authors.affiliation: Center for Jeongup Industry-Academy-Institute Cooperation, Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeollabuk-do 54896, Republic of Korea. Electronic address: tgkim@jbnu.ac.kr.
authors.forename: Tae-Geum
authors.initials: TG
authors.lastname: Kim
mesh_terms: D002772:Cholera Toxin / Q000096:biosynthesis / Q000235:genetics; D006023:Glycoproteins / Q000096:biosynthesis / Q000235:genetics; D029141:Novirhabdovirus / Q000235:genetics* / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D006023:Glycoproteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D002772:Cholera Toxin
keywords: Cholera toxin B subunit; Glycoprotein; Plant-based vaccine; Transient expression; Viral hemorrhagic septicemia virus