title: YghJ, the secreted metalloprotease of pathogenic E. coli induces hemorrhagic fluid accumulation in mouse ileal loop.
abstract: YghJ, also known as SslE (Secreted and surface associated lipoprotein) is a cell surface associated and secreted lipoprotein harbouring M60 metalloprotease domain. Though the gene is known to be conserved among both pathogenic and commensal Escherichia coli isolates, the expression and secretion of YghJ was found to be higher among diverse E. coli pathotypes. YghJ, secreted from intestinal pathogens such as enterotoxigenic E. coli (ETEC) and enteropathogenic E. coli (EPEC) has been demonstrated to possess mucinase activity and hence facilitates colonization of these enteric pathogens to intestinal epithelial cells. Importantly, YghJ is also reported to be secreted from extraintestinal pathogenic E. coli isolates. In our previous study we have shown that YghJ, purified from a neonatal septicemic E. coli isolate could trigger induction of various proinflammatory cytokines in vitro. This led us to investigate the role of YghJ in causing in vivo tissue hemorrhage. In the present study, we validate the earlier in vitro finding and have showed that YghJ can cause extensive tissue damage in mouse ileum and is also able to induce significant fluid accumulation in a dose dependent manner in a mouse ileal loop (MIL) assay. Hence, our present study not only confirms the pathogenic potential of YghJ in sepsis pathophysiology but also indicates the enterotoxic ability of YghJ which makes it an important virulence determinant of intestinal pathogenic E. coli.
authors.affiliation: Division of Pathophysiology, National Institute of Cholera and Enteric Diseases (NICED), Kolkata 10, India.
authors.forename: Rima
authors.initials: R
authors.lastname: Tapader
authors.affiliation: Centre for Infectious Diseases & Control, School of Biosciences and Technology, VIT University, Vellore 632014, India.
authors.forename: Dipro
authors.initials: D
authors.lastname: Bose
authors.affiliation: Division of Pathophysiology, National Institute of Cholera and Enteric Diseases (NICED), Kolkata 10, India. Electronic address: apal7364@rediffmail.com.
authors.forename: Amit
authors.initials: A
authors.lastname: Pal
mesh_terms: D000818:Animals; D004926:Escherichia coli / Q000201:enzymology* / Q000472:pathogenicity*; D029968:Escherichia coli Proteins / Q000633:toxicity*; D006470:Hemorrhage / Q000139:chemically induced*; D007082:Ileum / Q000382:microbiology* / Q000473:pathology*; D045726:Metalloproteases / Q000633:toxicity*; D008807:Mice, Inbred BALB C; D037521:Virulence Factors / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D037521:Virulence Factors; D045726:Metalloproteases; C587633:YghJ protein, E coli
keywords: Hemorrhagic fluid response; Intestinal pathogenic E. coli; Mouse ileal loop; Neonatal septicemic E. coli; YghJ (SslE)