title: Small RNAs Derived from the T-DNA ofAgrobacterium rhizogenesin Hairy Roots ofPhaseolus vulgaris.
abstract: Agrobacterium rhizogenesis a pathogenic bacteria that causes hairy root disease by transferring bacterial DNA into the plant genome. It is an essential tool for industry and research due to its capacity to produce genetically modified roots and whole organisms. Here, we identified and characterized small RNAs generated from the transfer DNA (T-DNA) ofA. rhizogenesin hairy roots of common bean (Phaseolus vulgaris). Distinct abundantA. rhizogenesT-DNA-derived small RNAs (ArT-sRNAs) belonging to several oncogenes were detected in hairy roots using high-throughput sequencing. The most abundant and diverse species of ArT-sRNAs were those of 21- and 22-nucleotides in length. Many T-DNA encoded genes constituted phasiRNA producing loci (PHASloci). Interestingly, degradome analysis revealed that ArT-sRNAs potentially target genes ofP. vulgaris. In addition, we detected low levels of ArT-sRNAs in theA. rhizogenes-induced calli generated at the wound site before hairy root emergence. These results suggest that RNA silencing targets several genes from T-DNA ofA. rhizogenesin hairy roots of common bean. Therefore, the role of RNA silencing observed in this study has implications in our understanding and usage of this unique plant-bacteria interaction.
authors.affiliation: Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de MéxicoCuernavaca, Mexico; Laboratorio Nacional de Genómica para la Biodiversidad, Unidad de Genómica Avanzada del Centro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalIrapuato, Mexico.
authors.forename: Pablo
authors.initials: P
authors.lastname: Peláez
authors.affiliation: Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México Cuernavaca, Mexico.
authors.forename: Alejandrina
authors.initials: A
authors.lastname: Hernández-López
authors.affiliation: Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México Cuernavaca, Mexico.
authors.forename: Georgina
authors.initials: G
authors.lastname: Estrada-Navarrete
authors.affiliation: Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México Cuernavaca, Mexico.
authors.forename: Federico
authors.initials: F
authors.lastname: Sanchez
keywords: Agrobacterium rhizogenes; RNA silencing; T-DNA; hairy roots; small RNAs