title: Enhanced repair of segmental bone defects in rabbit radius by porous tantalum scaffolds modified with the RGD peptide.
abstract: Fast and stable repair of segmental bone defects remains a challenge for clinical orthopedic surgery. In recent years, porous tantalum has been widely applied in clinical orthopedics for low modulus of elasticity, with three-dimensional microstructures similar to cancellous bone and excellent biocompatibility. To further improve bone the repairing ability of porous tantalum, the cyclo(-RGDfK-) peptide was coated on the surface of porous tantalum scaffolds. A model of 15 mm segmental defect was made at the midshaft of right radius in New Zealand White rabbits. In the experimental group, defects were implanted (press-fit) using porous tantalum scaffolds modified with cyclo(-RGDfK-) ﻿peptide﻿. Control animals were implanted with non-modified porous tantalum scaffolds or xenogeneic cancellous bone scaffolds, respectively. No implant was provided for the blank group. Bone repair was assessed by X-ray and histological observations at 4, 8, and 16 weeks post-operation, with biomechanical tests and micro-computed tomography performed at 16 weeks post-surgery. The results showed that bone formation was increased at the interface and inside the inner pores of modified porous tantalum scaffolds than those of non-modified porous tantalum scaffolds; biomechanical properties in the modified porous tantalum group were superior to those of the non-modified porous tantalum and xenogeneic cancellous bone groups, while new bone volume fractions using micro-computed tomography analysis were similar between the modified porous tantalum and xenogeneic cancellous bone groups. Our findings suggested that modified porous tantalum scaffolds had enhanced repairing ability in segmental bone defect in rabbit radius, and may serve as a potential material for repairing large bone defects.
authors.affiliation: Hand Surgery Department, Tangshan orthopaedic hospital affiliated, North China University of Science and Technology, Tangshan, Hebei, 063000, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Experimental Center, North China University of Science and Technology, Tangshan, Hebei, 063000, P.R. China.
authors.forename: Qijia
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Anatomy, Basic Medical College, North China University of Science and Technology, Tangshan, Hebei, 063000, P.R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Joint Surgery, Tangshan orthopaedic hospital affiliated, North China University of Science and Technology, Tangshan, Hebei, 063000, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedics, Affiliated Hospital, North China University of Science and Technology, Tangshan, Hebei, 063000, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.affiliation: Department of Orthopaedics, Affiliated Hospital, North China University of Science and Technology, Tangshan, Hebei, 063000, P.R. China.
authors.forename: Hongquan
authors.initials: H
authors.lastname: Gan
authors.affiliation: Department of Orthopaedics, Affiliated Hospital, North China University of Science and Technology, Tangshan, Hebei, 063000, P.R. China.
authors.forename: Huiping
authors.initials: H
authors.lastname: Song
authors.affiliation: Department of Orthopaedics, Affiliated Hospital, North China University of Science and Technology, Tangshan, Hebei, 063000, P.R. China. wanghuiyxx@163.com.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D018786:Bone Substitutes / Q000737:chemistry*; D004548:Elasticity; D008297:Male; D009842:Oligopeptides / Q000737:chemistry*; D009985:Orthopedics; D016062:Porosity; D011312:Pressure; D011817:Rabbits; D011884:Radius / Q000502:physiology / Q000601:surgery*; D013314:Stress, Mechanical; D013635:Tantalum / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014057:Tomography, X-Ray Computed; D055114:X-Ray Microtomography
chemical_list: D018786:Bone Substitutes; D009842:Oligopeptides; D013635:Tantalum; C047981:arginyl-glycyl-aspartic acid