title: TRIM14 regulates cell proliferation and invasion in osteosarcoma via promotion of the AKT signaling pathway.
abstract: Recent studies have shown that some members of the tripartite motif-containing protein (TRIM) family serve as important regulators of tumorigenesis. However, the biological role of TRIM14 in osteosarcoma remains to be established. In this study, we showed that TRIM14 is upregulated in human osteosarcoma specimens and cell lines, and correlated with osteosarcoma progression and shorter patient survival times. Functional studies demonstrated that overexpression of TRIM14 enhances osteosarcoma cell proliferation, clone formation, cell cycle procession, migration and invasion in vitro and promotes tumor growth in vivo, and conversely, its silencing has the opposite effects. Furthermore, TRIM14 overexpression induced activation of the AKT pathway. Inhibition of AKT expression reversed the TRIM14-mediated promotory effects on cell growth and mobility, in addition to TRIM14-induced epithelial-to-mesenchymal transition (EMT) and cyclin D1 upregulation. Our findings collectively suggest that TRIM14 functions as an oncogene by upregulating the AKT signaling pathway in osteosarcoma cells, supporting its potential utility as a therapeutic target for this disease.
authors.affiliation: Department of Biophysics, Second Military Medical University, No. 800 Xiangyin Road, 200433, Shanghai, People's Republic of China.
authors.forename: Guoxing
authors.initials: G
authors.lastname: Xu
authors.affiliation: Department of Orthopedics, ChangZheng Hospital, Second Military Medical University, No. 415 Fengyang Road, 200003, Shanghai, People's Republic of China.
authors.forename: Yongfei
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Orthopedics, Third Affiliated Hospital, Second Military Medical University, No. 700 Moyu North Road, 201805, Shanghai, People's Republic of China.
authors.forename: Dabo
authors.initials: D
authors.lastname: Xu
authors.affiliation: Department of Orthopedics, Third Affiliated Hospital, Second Military Medical University, No. 700 Moyu North Road, 201805, Shanghai, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Biophysics, Second Military Medical University, No. 800 Xiangyin Road, 200433, Shanghai, People's Republic of China.
authors.forename: Yafeng
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Biophysics, Second Military Medical University, No. 800 Xiangyin Road, 200433, Shanghai, People's Republic of China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Biophysics, Second Military Medical University, No. 800 Xiangyin Road, 200433, Shanghai, People's Republic of China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Lv
authors.affiliation: Department of Orthopedics, Third Affiliated Hospital, Second Military Medical University, No. 700 Moyu North Road, 201805, Shanghai, People's Republic of China.
authors.forename: Fanglong
authors.initials: F
authors.lastname: Song
authors.affiliation: Department of Orthopedics, Third Affiliated Hospital, Second Military Medical University, No. 700 Moyu North Road, 201805, Shanghai, People's Republic of China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Jiang
authors.affiliation: Department of Orthopedics, Third Affiliated Hospital, Second Military Medical University, No. 700 Moyu North Road, 201805, Shanghai, People's Republic of China.
authors.forename: Yinquan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, Third Affiliated Hospital, Second Military Medical University, No. 700 Moyu North Road, 201805, Shanghai, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Lou
authors.affiliation: Department of Orthopedics, ChangZheng Hospital, Second Military Medical University, No. 415 Fengyang Road, 200003, Shanghai, People's Republic of China.
authors.forename: Yake
authors.initials: Y
authors.lastname: Meng
authors.affiliation: Department of Biophysics, Second Military Medical University, No. 800 Xiangyin Road, 200433, Shanghai, People's Republic of China.
authors.forename: Yongji
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Orthopedics, Third Affiliated Hospital, Second Military Medical University, No. 700 Moyu North Road, 201805, Shanghai, People's Republic of China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Kang
mesh_terms: D000328:Adult; D000818:Animals; D001859:Bone Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D002352:Carrier Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D064593:Heterografts; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D008875:Middle Aged; D060787:Neoplasm Grading; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D012516:Osteosarcoma / Q000235:genetics* / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D011379:Prognosis; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction; D000070584:Tripartite Motif Proteins; D047368:Tumor Burden; D055815:Young Adult
chemical_list: D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; C481145:TRIM14 protein, human; D000070584:Tripartite Motif Proteins; D051057:Proto-Oncogene Proteins c-akt