title: Detecting RNA modifications in the epitranscriptome: predict and validate.
abstract: RNA modifications are emerging players in the field of post-transcriptional regulation of gene expression, and are attracting a comparable degree of research interest to DNA and histone modifications in the field of epigenetics. We now know of more than 150 RNA modifications and the true potential of a few of these is currently emerging as the consequence of a leap in detection technology, principally associated with high-throughput sequencing. This Review outlines the major developments in this field through a structured discussion of detection principles, lays out advantages and drawbacks of new high-throughput methods and presents conventional biophysical identification of modifications as meaningful ways for validation.
authors.affiliation: Institute of Pharmacy and Biochemistry, Johannes Gutenberg University Mainz, Staudingerweg 5, 55128 Mainz, Germany.
authors.forename: Mark
authors.initials: M
authors.lastname: Helm
authors.affiliation: IMoPA UMR7365 CNRS-UL, BioPole Lorraine University, 9 avenue de la Foret de Haye, 54505 Vandoeuvre-les-Nancy, France.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Motorin
mesh_terms: D000818:Animals; D044127:Epigenesis, Genetic / Q000235:genetics*; D057890:Epigenomics / Q000379:methods*; D005786:Gene Expression Regulation; D006801:Humans; D012313:RNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D059467:Transcriptome / Q000235:genetics*
chemical_list: D012313:RNA