title: Meta-analysis Reveals No Association of DNMT3B -149 C>T Gene Polymorphism With Overall Cancer Risk.
abstract: BACKGROUND
  DNA methyltransferase-3B (DNMT3B) plays a key role in establishment and maintenance of genomic methylation patterns. Polymorphism in promoter region -149 C>T (C46359T) of DNMT3B gene may alter DNMT3B activity which leads to increased susceptibility to cancer. Inconsistent results regarding this have been reported in a number of studies.
  
  
  OBJECTIVE
  To carry out a meta-analysis of the studies reported to assess the precise relationship between the DNMT3B -149 C>T polymorphism and the overall cancer risk.
  
  
  METHODS
  PubMed (MEDLINE) web database was searched for the studies concerning DNMT3B -149 C>T polymorphism and its association with cancer risk. The pooled odds ratios (ORs) along with 95% confidence intervals (95% CIs) were calculated for all the genetic models, from the selected case-control studies, by meta-analysis.
  
  
  RESULTS
  Overall eighteen studies containing 5583 cancer cases and 7618 controls were analyzed. No significant risk was observed overall for T allele carrier (T vs. C: p=0.303; OR=1.032, 95% CI=0.972-1.097), homozygous (TT vs. CC: p=0.336; OR=1.063, 95% CI=0.939-1.204), heterozygous (CT vs. CC: p=0.802; OR=1.022, 95% CI=0.860-1.216), dominant (TT vs. CC+CT: p=0.298; OR=1.101, 95% CI=0.919-1.319) and recessive (TT+CT vs. CC: p=0.656; OR=1.021, 95% CI=0.931-1.121) genetic models. Subgroup analysis of Asian and Caucasian populations also did not demonstrate any cancer risk in all the genetic models studied.
  
  
  CONCLUSIONS
  Our meta-analysis proposes that the DNMT3B -149 C>T polymorphism may not be an independent predisposing factor for the risk of cancer. However, larger sample size and expression studies are required to confirm the observation.
authors.affiliation: Research and Scientific Studies Unit, College of Nursing and Allied Health Sciences, Jazan University, Jazan-45142, Kingdom of Saudi Arabia.
authors.forename: Raju Kumar
authors.initials: RK
authors.lastname: Mandal
authors.affiliation: Research and Scientific Studies Unit, College of Nursing and Allied Health Sciences, Jazan University, Jazan-45142, Kingdom of Saudi Arabia;; Department of Biosciences, Jamia Millia Islamia (A Central University), New Delhi-110025, India.
authors.forename: Shafiul
authors.initials: S
authors.lastname: Haque
authors.affiliation: Research and Scientific Studies Unit, College of Nursing and Allied Health Sciences, Jazan University, Jazan-45142, Kingdom of Saudi Arabia;; Department of Biosciences, Jamia Millia Islamia (A Central University), New Delhi-110025, India.
authors.forename: Mohd
authors.initials: M
authors.lastname: Wahid
authors.affiliation: Research and Scientific Studies Unit, College of Nursing and Allied Health Sciences, Jazan University, Jazan-45142, Kingdom of Saudi Arabia.
authors.forename: Arshad
authors.initials: A
authors.lastname: Jawed
authors.affiliation: Department of Biosciences, Jamia Millia Islamia (A Central University), New Delhi-110025, India;; Department of Laboratory Medicine, Faculty of Applied Medical Sciences, Albaha University, Albaha, Saudi Arabia.
authors.forename: Naseem
authors.initials: N
authors.lastname: Akhter
authors.affiliation: Centre for Life Science, School of Natural Sciences, Central University of Jharkhand, Ranchi-835205, Jharkhand, India.
authors.forename: Md Ekhlaque Ahmed
authors.initials: ME
authors.lastname: Khan
authors.affiliation: Centre for Life Science, School of Natural Sciences, Central University of Jharkhand, Ranchi-835205, Jharkhand, India.
authors.forename: Aditya Kumar
authors.initials: AK
authors.lastname: Panda
authors.affiliation: Research and Scientific Studies Unit, College of Nursing and Allied Health Sciences, Jazan University, Jazan-45142, Kingdom of Saudi Arabia.
authors.forename: Mohammed Yahya
authors.initials: MY
authors.lastname: Areeshi
authors.affiliation: Research and Scientific Studies Unit, College of Nursing and Allied Health Sciences, Jazan University, Jazan-45142, Kingdom of Saudi Arabia.
authors.forename: Sajad Ahmad
authors.initials: SA
authors.lastname: Dar
keywords: Asians; Cancer; Caucasians; DNA methyltransferase-3B; Meta-analysis; Polymorphism