title: Comparison of bioactive components and pharmacological activities of ophiopogon japonicas extracts from different geographical origins.
abstract: Ophiopogon japonicus (Linn. f.) Ker-Gawl (O. japonicas), mainly cultivated in Sichuan and Zhejiang province in China, has different bioactive components and therefore their pharmacological activities. To explain the different clinical efficacy of O. japonicas derived preparations, herein we report differences of pharmacological activities between Sichuan and Zhejiang O. japonicas and behind them the exact differences of bioactive components. Based on a LC/MS-IT-TOF method, the differences of bioactive components between Sichuan and Zhejiang O. japonicas extracts were analyzed and respective characteristic components were picked out. We determined 39 ophiopogonones and 71 ophiopogonins compounds in Sichuan and Zhejiang O. japonicas extracts and found the contents of these compositions have several times difference. Evidenced by experimental data of pharmacological activities in inhibiting cardiomyocyte damage induced by H2O2, mouse macrophage cell inflammation induced by lipopolysaccharide and cytotoxicity in vitro, Zhejiang O. japonicas extract had a stronger antioxidant and anti-inflammatory capacity than Sichuan O. japonicas extract, and the two O. japonicas extracts exhibited selective cytotoxicity on different cancer cell lines in vitro. These data shed light on the links between bioactive components and pharmacological activities of O. japonicas derived preparations. Thus, geographical origin of O. japonicas should be considered to be a key factor in efficacy studies and further clinical application.
authors.affiliation: State Key Laboratory of Natural Medicines, Key Lab of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, Tongjiaxiang 24, Nanjing, 21009, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Natural Medicines, Key Lab of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, Tongjiaxiang 24, Nanjing, 21009, China.
authors.forename: Wan-Feng
authors.initials: WF
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Natural Medicines, Key Lab of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, Tongjiaxiang 24, Nanjing, 21009, China.
authors.forename: Han-Yuan
authors.initials: HY
authors.lastname: Shen
authors.affiliation: ChiaTai Qingchunbao Pharmaceutical Co. Ltd., Xixi Road 551, Xihu District, Hangzhou, China.
authors.forename: Pei-Qiang
authors.initials: PQ
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Natural Medicines, Key Lab of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, Tongjiaxiang 24, Nanjing, 21009, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Natural Medicines, Key Lab of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, Tongjiaxiang 24, Nanjing, 21009, China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Wang
authors.affiliation: ChiaTai Qingchunbao Pharmaceutical Co. Ltd., Xixi Road 551, Xihu District, Hangzhou, China.
authors.forename: Han
authors.initials: H
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Natural Medicines, Key Lab of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, Tongjiaxiang 24, Nanjing, 21009, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Natural Medicines, Key Lab of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, Tongjiaxiang 24, Nanjing, 21009, China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Natural Medicines, Key Lab of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, Tongjiaxiang 24, Nanjing, 21009, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Natural Medicines, Key Lab of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, Tongjiaxiang 24, Nanjing, 21009, China.
authors.forename: Hai-Ping
authors.initials: HP
authors.lastname: Hao
authors.affiliation: State Key Laboratory of Natural Medicines, Key Lab of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, Tongjiaxiang 24, Nanjing, 21009, China. Electronic address: guangjiwang@hotmail.com.
authors.forename: Guang-Ji
authors.initials: GJ
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Natural Medicines, Key Lab of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, Tongjiaxiang 24, Nanjing, 21009, China. Electronic address: caolijuan0702@cpu.edu.cn.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Cao
mesh_terms: D000072283:A549 Cells; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D045744:Cell Line, Tumor; D002681:China; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology*; D045325:HCT116 Cells; D019073:HT29 Cells; D056945:Hep G2 Cells; D006801:Humans; D061986:MCF-7 Cells; D031441:Ophiopogon / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D051381:Rats
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts
keywords: Anti-cancer; Anti-inflammatory; Antioxidant; Bioactive components; LC/MS-IT-TOF; Sichuan and Zhejiang O. japonicas extracts