title: 4-hydroxybenzaldehyde-chitooligomers suppresses H2O2-induced oxidative damage in microglia BV-2 cells.
abstract: Positive charges of chitooligomer (COS) enable COS to interact with negatively charged anionic groups on the cell surface resulting in an improvement in the biological activity of COS and its derivatives. In this study, 4-hydroxybenzaldehyde-COS (HB-COS) was synthesized and investigated for its abilities against H2O2-induced oxidative stress in microglia BV-2 cells. Under oxidative stress, HB-COS significantly attenuated reactive oxygen species (ROS) generation and DNA oxidation, and upregulated the protein levels of antioxidative enzymes. HB-COS is therefore proposed as a potential protective agent against neuronal damage.
authors.affiliation: Marine Bioprocess Research Center, Pukyong National University, Busan, 608-739, Republic of Korea.
authors.forename: Sea-Hun
authors.initials: SH
authors.lastname: Oh
authors.affiliation: School of Pharmacy, The University of Queensland, Brisbane, QLD, 4072, Australia.
authors.forename: BoMi
authors.initials: B
authors.lastname: Ryu
authors.affiliation: Faculty of Resources and Environment, Thu Dau Mot University, Binh Duong, Viet Nam.
authors.forename: Dai-Hung
authors.initials: DH
authors.lastname: Ngo
authors.affiliation: Major in Pharmaceutical Engineering Division of Bio-Industry, Silla University, Busan, Republic of Korea.
authors.forename: Won-Suk
authors.initials: WS
authors.lastname: Kim
authors.affiliation: Specialized Graduate School Science and Technology Convergence, Department of Marine Bio Convergence Science, Pukyong National University, Busan, 608-737, Republic of Korea. Electronic address: dgkim@pknu.ac.kr.
authors.forename: Dong Gyu
authors.initials: DG
authors.lastname: Kim
authors.affiliation: Marine Bioprocess Research Center, Pukyong National University, Busan, 608-739, Republic of Korea; Specialized Graduate School Science and Technology Convergence, Department of Marine Bio Convergence Science, Pukyong National University, Busan, 608-737, Republic of Korea. Electronic address: sknkim@pknu.ac.kr.
authors.forename: Se-Kwon
authors.initials: SK
authors.lastname: Kim
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000138:chemical synthesis / Q000494:pharmacology*; D001547:Benzaldehydes / Q000737:chemistry / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000187:drug effects; D002686:Chitin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D048271:Chitosan; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D051379:Mice; D017628:Microglia / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D009844:Oligosaccharides; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism; D055672:Static Electricity; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001547:Benzaldehydes; D005452:Fluoresceins; D005456:Fluorescent Dyes; D018696:Neuroprotective Agents; D009844:Oligosaccharides; D017382:Reactive Oxygen Species; C493484:oligochitosan; D002686:Chitin; C029569:diacetyldichlorofluorescein; D048271:Chitosan; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005978:Glutathione; C011483:4-hydroxybenzaldehyde
keywords: 4-hydroxybenzaldehyde-COS (HB-COS); Microglia BV-2 cells; Oxidative stress