title: Determination of VEGFR-2 (KDR) 604A>G Polymorphism in Pancreatic Disorders.
abstract: Pancreatic disorders have a high prevalence worldwide. Despite the fact that screening methods became more effective and the knowledge we have nowadays about pancreatic diseases has enhanced, their incidence remains high. Our purpose was to determine whether single nucleotide polymorphism (SNP) of VEGFR-2/KDR (vascular endothelial growth factor receptor 2/kinase insert domain receptor) influences susceptibility to develop pancreatic pathology. Genomic DNA was extracted from blood samples collected from patients diagnosed with acute pancreatitis (n= 110), chronic pancreatitis (n= 25), pancreatic cancer (n= 82) and healthy controls (n= 232). VEGFR-2 (KDR) 604A>G (rs2071559) polymorphism frequency was determined with TaqMan allelic discrimination assays. Statistical assessment was performed by associating genetic polymorphism with clinical and pathological data. In both pancreatic disorders and healthy control groups the polymorphism we studied was in Hardy-Weinberg equilibrium. Association between increased risk for pancreatic disorders and studied polymorphism was statistically significant. KDR 604AG and AG + GG genotypes were more prevalent in acute pancreatitis and pancreatic cancer patients than in controls. These genotypes influence disease development in a low rate. No association was found between chronic pancreatitis and KDR 604AG and AG + GG genotypes. In Romanian cohort, we found an association between the KDR 604A→Gpolymorphism and acute pancreatitis and pancreatic cancer. Carriers of the -604G variant allele were more frequent among acute pancreatitis and pancreatic cancer than among controls, suggesting that KDR 604G allele may confer an increased risk for these diseases. In the future, more extensive studies on larger groups are necessary, in order to clarify the role of VEGFR2 polymorphisms in pancreatic pathology.
authors.affiliation: Department of Internal Medicine, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania. vldpadureanu@yahoo.com.
authors.forename: Vlad
authors.initials: V
authors.lastname: Pădureanu
authors.affiliation: Department of Immunology, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania. boldeanumihailvirgil@yahoo.com.|Medico Science SRL-Stem Cell Bank Unit, 1B Brazda lui Novac Street, 200690 Craiova, Romania. boldeanumihailvirgil@yahoo.com.
authors.forename: Mihail Virgil
authors.initials: MV
authors.lastname: Boldeanu
authors.affiliation: Human Genomics Laboratory, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania. ioana.streata@yahoo.com.
authors.forename: Ioana
authors.initials: I
authors.lastname: Streaţă
authors.affiliation: Human Genomics Laboratory, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania. cucu_mihai_gabriel@yahoo.com.
authors.forename: Mihai Gabriel
authors.initials: MG
authors.lastname: Cucu
authors.affiliation: Department of Immunology, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania. isabela_silosi@yahoo.com.
authors.forename: Isabela
authors.initials: I
authors.lastname: Siloşi
authors.affiliation: Medico Science SRL-Stem Cell Bank Unit, 1B Brazda lui Novac Street, 200690 Craiova, Romania. barulidia@yahoo.com.
authors.forename: Lidia
authors.initials: L
authors.lastname: Boldeanu
authors.affiliation: Maria Bogdan, Department of Pharmacology, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania. bogdanfmaria81@yahoo.com.
authors.forename: Maria
authors.initials: M
authors.lastname: Bogdan
authors.affiliation: Department of Anatomy, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania. ancaenescus@yahoo.com.
authors.forename: Anca Ştefania
authors.initials: AŞ
authors.lastname: Enescu
authors.affiliation: Department of Medico-Surgical Emergencies, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania. m_maria2521@yahoo.com.
authors.forename: Maria
authors.initials: M
authors.lastname: Forţofoiu
authors.affiliation: Department of Medico-Surgical Emergencies, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania. sea180385@gmail.com.
authors.forename: Aurelia
authors.initials: A
authors.lastname: Enescu
authors.affiliation: Faculty of Nursing and Midwifery, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania. madeldum@yahoo.com.
authors.forename: Elena Mădălina
authors.initials: EM
authors.lastname: Dumitrescu
authors.affiliation: Department of Medical Informatics and Biostatistics, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania. dragosado@yahoo.com.
authors.forename: Dragoş
authors.initials: D
authors.lastname: Alexandru
authors.affiliation: Department of Surgery, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania. vsurlin@gmail.com.
authors.forename: Valeriu Marian
authors.initials: VM
authors.lastname: Şurlin
authors.affiliation: Department of Internal Medicine, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania. catalin1972@hotmail.com.
authors.forename: Mircea Cătălin
authors.initials: MC
authors.lastname: Forţofoiu
authors.affiliation: Department of Pediatrics, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania. petrescu.ileanaoctavia@yahoo.com.
authors.forename: Ileana Octavia
authors.initials: IO
authors.lastname: Petrescu
authors.affiliation: Department of Internal Medicine, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania. petrescu.florin@yahoo.com.
authors.forename: Florin
authors.initials: F
authors.lastname: Petrescu
authors.affiliation: Human Genomics Laboratory, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania. mihaiioana_romania@yahoo.com.
authors.forename: Mihai
authors.initials: M
authors.lastname: Ioana
authors.affiliation: Department of Plastic Surgery, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania. meciurea@gmail.com.
authors.forename: Marius Eugen
authors.initials: ME
authors.lastname: Ciurea
authors.affiliation: Research Center of Gastroenterology and Hepatology Craiova, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania. adrian.saftoiu@umfcv.ro.|Visiting Clinical Professor, Gastrointestinal Unit, Copenhagen University Hospital Herlev, 2730 Herlev, Denmark. adrian.saftoiu@umfcv.ro.
authors.forename: Adrian
authors.initials: A
authors.lastname: Săftoiu
mesh_terms: D016022:Case-Control Studies; D003710:Demography; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010182:Pancreatic Diseases / Q000175:diagnosis / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000235:genetics*
chemical_list: D040301:Vascular Endothelial Growth Factor Receptor-2
keywords: VEGFR-2; genotype; pancreatic disorders; polymorphism