title: Assisted reproductive technologies in the common marmoset: an integral species for developing nonhuman primate models of human diseases.
abstract: Generation of nonhuman primate models of human disease conditions will foster the development of novel therapeutic strategies. Callithrix jacchus, or the common marmoset, is a New World, nonhuman primate species that exhibits great reproductive fitness in captivity with an ovarian cycle that can be easily managed with pharmacological agents. This characteristic, among others, provides an opportunity to employ assisted reproductive technologies to generate embryos that can be genetically manipulated to create a variety of nonhuman primate models for human disease. Here, we review methods to synchronize the marmoset ovarian cycle and stimulate oocyte donors, and compare various protocols for in vitro production of embryos. In light of advances in genomic editing, recent approaches used to generate transgenic or genetically edited embryos in the marmoset and also future perspective are reviewed.
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Jenna
authors.initials: J
authors.lastname: Kropp
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Andrea
authors.initials: A
authors.lastname: Di Marzo
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, USA.|Department of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA.|Department of Obstetrics and Gynecology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Thaddeus
authors.initials: T
authors.lastname: Golos
mesh_terms: D000818:Animals; D002144:Callithrix / Q000502:physiology*; D004195:Disease Models, Animal; D046149:Embryo Culture Techniques / Q000662:veterinary; D004622:Embryo, Mammalian / Q000502:physiology*; D005260:Female; D008597:Menstrual Cycle / Q000502:physiology*; D027724:Reproductive Techniques, Assisted / Q000662:veterinary*
keywords: in vitro fertilization; marmoset; transgenesis; genomic editing