title: Optogenetic inhibition of apical constriction during Drosophila embryonic development.
abstract: Morphogenesis of multicellular organisms is driven by changes in cell behavior, which happen at precise locations and defined developmental stages. Therefore, the studying of morphogenetic events would greatly benefit from tools that allow the perturbation of cell activity with spatial and temporal precision. We recently developed an optogenetic approach to modulate cell contractility with cellular precision and on fast (seconds) timescales during Drosophila embryogenesis. We present here a protocol to handle genetically engineered photosensitive Drosophila embryos and achieve light-mediated inhibition of apical constriction during tissue invagination. The possibility to modulate the levels of optogenetic activation at different laser powers makes this method suited also for studying how mechanical stresses are sensed and interpreted in vivo. Given the conserved function of cell contractility during animal development, the application of this method to other morphogenetic processes will facilitate our understanding of tissue mechanics and cell-cell interaction during morphogenesis.
authors.affiliation: European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Guglielmi
authors.affiliation: European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
authors.forename: S
authors.initials: S
authors.lastname: De Renzis
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D048430:Cell Shape / Q000235:genetics; D004330:Drosophila / Q000254:growth & development / Q000648:ultrastructure*; D004625:Embryo, Nonmammalian / Q000648:ultrastructure*; D047108:Embryonic Development / Q000235:genetics; D007834:Lasers; D009024:Morphogenesis / Q000235:genetics*; D062308:Optogenetics / Q000379:methods*
keywords: Actin; Apical constriction; Cell contractility; Cry2-CIB1; Drosophila; Optogenetics; Phosphoinositides; Tissue invagination; Tissue morphogenesis; Two photon