title: Laser deposition of poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) - lysozyme microspheres based coatings with anti-microbial properties.
abstract: The purpose of this study was to obtain, characterize and evaluate the cytotoxicity and antimicrobial activity of coatings based on poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) - Lysozyme (P(3HB-3HV)/Lys) and P(3HB-3HV) - Polyethylene glycol - Lysozyme (P(3HB-3HV)/PEG/Lys) spheres prepared by Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique, in order to obtain functional and improved Ti-based implants. Morphological investigation of the coatings by Infrared Microscopy (IRM) and SEM revealed that the average diameter of P(3HB-3HV)/Lys spheres is around 2μm and unlike the drop cast samples, IRM recorded on MAPLE films revealed a good distribution of monitored functional groups on the entire scanned surface. The biological evaluation of MAPLE structured surfaces revealed an improved biocompatibility with respect to osteoblasts and endothelial cells as compared with Ti substrates and an enhanced anti-biofilm effect against Gram positive (Staphylococcus aureus) and Gram negative (Pseudomonas aeruginosa) tested strains. Thus, we propose that the fabricated P(3HB-3HV)/PEG/Lys and P(3HB-3HV)/Lys microspheres may be efficiently used as a matrix for controlled local drug delivery, with practical applications in developing improved medical surfaces for the reduction of implant-associated infections.
authors.affiliation: National Institute for Lasers, Plasma & Radiation Physics, Lasers Department, P.O. Box MG-36, Bucharest-Magurele, Bucharest 769231, Romania; Politehnica University of Bucharest, Faculty of Applied Chemistry and Materials Science, Department of Science and Engineering of Oxide Materials and Nanomaterials, Polizu Street no 1-7, Bucharest 011061, Romania.
authors.forename: V
authors.initials: V
authors.lastname: Grumezescu
authors.affiliation: Politehnica University of Bucharest, Faculty of Applied Chemistry and Materials Science, Department of Science and Engineering of Oxide Materials and Nanomaterials, Polizu Street no 1-7, Bucharest 011061, Romania; University of Bucharest, Faculty of Biology, Microbiology Immunology Department, Aleea Portocalelor no 1-3, Bucharest 060101, Romania.
authors.forename: A M
authors.initials: AM
authors.lastname: Holban
authors.affiliation: Institute of Biochemistry, Romanian Academy, 296 Splaiul Independentei, 060031 Bucharest, Romania.
authors.forename: L E
authors.initials: LE
authors.lastname: Sima
authors.affiliation: Institute of Biochemistry, Romanian Academy, 296 Splaiul Independentei, 060031 Bucharest, Romania.
authors.forename: M B
authors.initials: MB
authors.lastname: Chiritoiu
authors.affiliation: Institute of Biochemistry, Romanian Academy, 296 Splaiul Independentei, 060031 Bucharest, Romania.
authors.forename: G N
authors.initials: GN
authors.lastname: Chiritoiu
authors.affiliation: Research Institute of the University of Bucharest - ICUB, Bucharest, Romania.
authors.forename: A M
authors.initials: AM
authors.lastname: Grumezescu
authors.affiliation: Institute of Cellular Biology and Pathology "N. Simionescu", Proteomics Department, B. P. Hasdeu Street, No. 8, 050568 Bucharest, Romania.
authors.forename: L
authors.initials: L
authors.lastname: Ivan
authors.affiliation: Institute of Cellular Biology and Pathology "N. Simionescu", Proteomics Department, B. P. Hasdeu Street, No. 8, 050568 Bucharest, Romania.
authors.forename: F
authors.initials: F
authors.lastname: Safciuc
authors.affiliation: Institute of Cellular Biology and Pathology "N. Simionescu", Proteomics Department, B. P. Hasdeu Street, No. 8, 050568 Bucharest, Romania.
authors.forename: F
authors.initials: F
authors.lastname: Antohe
authors.affiliation: National Institute of Materials Physics, Magurele, Ilfov, Romania.
authors.forename: C
authors.initials: C
authors.lastname: Florica
authors.affiliation: National Institute for Lasers, Plasma & Radiation Physics, Lasers Department, P.O. Box MG-36, Bucharest-Magurele, Bucharest 769231, Romania.
authors.forename: C R
authors.initials: CR
authors.lastname: Luculescu
authors.affiliation: University of Bucharest, Faculty of Biology, Microbiology Immunology Department, Aleea Portocalelor no 1-3, Bucharest 060101, Romania.
authors.forename: M C
authors.initials: MC
authors.lastname: Chifiriuc
authors.affiliation: National Institute for Lasers, Plasma & Radiation Physics, Lasers Department, P.O. Box MG-36, Bucharest-Magurele, Bucharest 769231, Romania. Electronic address: gabriel.socol@inflpr.o.
authors.forename: G
authors.initials: G
authors.lastname: Socol
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D016503:Drug Delivery Systems; D006801:Humans; D007834:Lasers; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D009113:Muramidase / Q000737:chemistry* / Q000494:pharmacology; D011091:Polyesters / Q000737:chemistry* / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000737:chemistry; D000094122:Polyhydroxybutyrates
chemical_list: D000890:Anti-Infective Agents; D009113:Muramidase; D011091:Polyesters; D011092:Polyethylene Glycols; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates
keywords: Antimicrobial surfaces; Laser processing; Lysozyme; MAPLE thin coatings; Osteoblast and endothelial cell culture