title: Untargeted metabolomics analysis of adipogenic transformation in OP9-DL1 cells using liquid chromatography-mass spectrometry: Implications for thymic adipogenesis.
abstract: Adipocyte deposition is a key feature of age-related thymic involution, but the underlying mechanisms responsible for thymic adiposity remain to be elucidated. In the present study, we utilized rosiglitazone, a potent peroxisome proliferator-activated receptor γ agonist, to induce adipogenic differentiation of OP9-DL1 cells, and detected the metabolomics alterations during adipogenic differentiation by using liquid chromatography-mass spectrometry. The obtained metabolites were further processed by multivariate statistical analysis, including principal component analysis, partial least squares discriminant analysis, and orthogonal projection on latent-structures discriminant analysis. As a result, we identified a total of 33 significantly differential metabolites between dimethyl sulphoxide- and rosiglitazone-treated OP9-DL1 cells, which were closely related to the dysregulation of phospholipid metabolism pathway, oxidative stress, and associated amino acid metabolism. Meanwhile, two pathways including glycerophospholipid metabolism and nitrogen metabolism were significantly perturbed (P < 0.05). Collectively, our results may provide some heuristic guidance for addressing the underlying mechanism of thymic adipogenesis, and future studies are warranted to unravel the functions of these altered metabolites in thymic adipogenesis.
authors.affiliation: Research Center, Shengjing Hospital of China Medical University, Shenyang, 110004, People's Republic of China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Tan
authors.affiliation: Research Center, Shengjing Hospital of China Medical University, Shenyang, 110004, People's Republic of China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Medical Oncology, Shengjing Hospital of China Medical University, Shenyang, 110022, People's Republic of China.
authors.forename: Siliang
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Nerve Function, Shengjing Hospital of China Medical University, Shenyang, 110004, People's Republic of China.
authors.forename: Nannan
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Department of Blood Transfusion, Shengjing Hospital of China Medical University, Shenyang, 110022, People's Republic of China.
authors.forename: Simeng
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Blood Transfusion, Shengjing Hospital of China Medical University, Shenyang, 110022, People's Republic of China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: Research Center, Shengjing Hospital of China Medical University, Shenyang, 110004, People's Republic of China.
authors.forename: Xike
authors.initials: X
authors.lastname: Zhu
mesh_terms: D017667:Adipocytes / Q000502:physiology*; D050156:Adipogenesis; D000818:Animals; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D055442:Metabolome; D055432:Metabolomics; D051379:Mice; D018384:Oxidative Stress; D000077154:Rosiglitazone; D021241:Spectrometry, Mass, Electrospray Ionization; D045162:Thiazolidinediones / Q000494:pharmacology
chemical_list: D045162:Thiazolidinediones; D000077154:Rosiglitazone
keywords: LC-MS; OP9-DL1 cells; adipogenic differentiation; metabolomics; thymic adipogenesis