title: A novel antisense long non-coding RNA SATB2-AS1 overexpresses in osteosarcoma and increases cell proliferation and growth.
abstract: The knowledge regarding the importance of long non-coding RNAs (lncRNAs), a new class of genes, is very sparse in osteosarcoma. In the present study, we describe the expression profile of lncRNAs in osteosarcomas compared with paired adjacent non-cancerous tissue (n = 7) using microarray analysis. A total of 25,733 lncRNAs were identified in osteosarcoma; 1995 lncRNAs were consistently upregulated and 2226 lncRNAs were consistently under-regulated in all samples analyzed (≥2.0-fold, p < 0.05). We have validated three over-regulated and three under-regulated lncRNAs in patient samples (n = 7). The antisense transcript of SATB2 protein (SATB2-AS1) was identified as one of the upregulated lncRNAs. The SATB2-AS1 is a 3197-bp lncRNA on chromosome 2. This is the first report, where we have documented the increased expression of SATB2-AS1 in osteosarcoma patients and in human osteosarcoma cancer cell lines (U2OS, HOS, MG63). SATB2-AS1 expression was significantly higher in the metastatic tumors compared to non-metastatic tumors. In vitro gain and loss of function approaches demonstrated that SATB2-AS1 regulates cell cycle, cell proliferation, and cell growth. In addition, SATB2-AS1 affects the translational expression of SATB2 gene. Our data demonstrate that an antisense non-coding RNA regulates the expression of its sense gene, and increases the cell growth, therefore pointing the pivotal functions of SATB2-AS1 in osteosarcoma.
authors.affiliation: Jinan University, Guangzhou,, 510632, People's Republic of China.|Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou,, 510180, People's Republic of China.
authors.forename: Si-Hong
authors.initials: SH
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou,, 510180, People's Republic of China.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Zhu
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou,, 510180, People's Republic of China.
authors.forename: Hui-Hua
authors.initials: HH
authors.lastname: Xu
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou,, 510180, People's Republic of China.
authors.forename: Gui-Qiang
authors.initials: GQ
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou,, 510180, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou,, 510180, People's Republic of China.
authors.forename: Ya-Min
authors.initials: YM
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou,, 510180, People's Republic of China.
authors.forename: Jun-Bo
authors.initials: JB
authors.lastname: Liang
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou,, 510180, People's Republic of China.
authors.forename: Yu-Xuan
authors.initials: YX
authors.lastname: Wang
authors.affiliation: Department of Oncology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510180, People's Republic of China. wuyong@gzhmu.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou,, 510180, People's Republic of China. qfguo@gzhmu.edu.cn.
authors.forename: Qi-Feng
authors.initials: QF
authors.lastname: Guo
mesh_terms: D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D036961:Matrix Attachment Region Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism*; D062085:RNA, Long Noncoding / Q000096:biosynthesis* / Q000235:genetics; D012334:RNA, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics
chemical_list: D036961:Matrix Attachment Region Binding Proteins; D009363:Neoplasm Proteins; D062085:RNA, Long Noncoding; D012334:RNA, Neoplasm; C521297:SATB2 protein, human; D014157:Transcription Factors
keywords: Antisense; Cell Cycle; Cell proliferation; LncRNA; Metastasis; Osteosarcoma