title: Molecular characterization of the C-glucosylation for puerarin biosynthesis in Pueraria lobata.
abstract: C-glycosyltransferases (CGTs) are important enzymes that are responsible for the synthesis of the C-glycosides of flavonoids and isoflavonoids. Flavonoid CGTs have been molecularly characterized from several plant species; however, to date, no gene encoding an isoflavonoid CGT has been reported from any plant species. A significant example of an isoflavonoid C-glycoside is puerarin, a compound that contributes to the major medicinal effects of Pueraria lobata. Little is known about the C-glucosylation that occurs during puerarin biosynthesis. One possible route for puerarin synthesis is via the C-glucosylation of daidzein. This study describes the molecular cloning and functional characterization of a novel glucosyltransferase (PlUGT43) from P. lobata. Biochemical analyses revealed that PlUGT43 possesses an activity for the C-glucosylation of daidzein to puerarin; it shows activity with the isoflavones daidzein and genistein, but displays no activity towards other potential acceptors, including flavonoids. To validate the in vivo function of PlUGT43, the PlUGT43 gene was over-expressed in soybean hairy roots that naturally synthesize daidzein but that do not produce puerarin. The expression of PlUGT43 led to the production of puerarin in the transgenic soybean hairy roots, confirming a role for PlUGT43 in puerarin biosynthesis.
authors.affiliation: CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China.
authors.forename: Changfu
authors.initials: C
authors.lastname: Li
authors.affiliation: CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhou
authors.affiliation: CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.affiliation: CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China.|Sino-African Joint Research Center, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Yansheng
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism*; D007529:Isoflavones / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D029908:Pueraria / Q000235:genetics / Q000378:metabolism*
chemical_list: D007529:Isoflavones; D010940:Plant Proteins; C004742:daidzein; D016695:Glycosyltransferases; C033607:puerarin
keywords: 
  Pueraria lobata
  ; C-glucosyltransferase; isoflavone; soybean hairy roots; transcriptome