title: Engineering fatty acid synthases for directed polyketide production.
abstract: In this study, we engineered fatty acid synthases (FAS) for the biosynthesis of short-chain fatty acids and polyketides, guided by a combined in vitro and in silico approach. Along with exploring the synthetic capability of FAS, we aim to build a foundation for efficient protein engineering, with the specific goal of harnessing evolutionarily related megadalton-scale polyketide synthases (PKS) for the tailored production of bioactive natural compounds.
authors.affiliation: Institute of Organic Chemistry and Chemical Biology, Buchmann Institute for Molecular Life Sciences, Cluster of Excellence "Macromolecular Complexes," Goethe University Frankfurt, Frankfurt am Main, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Gajewski
authors.affiliation: Max Planck Institute for Biophysical Chemistry, Department of Theoretical and Computational Biophysics, Göttingen, Germany.
authors.forename: Floris
authors.initials: F
authors.lastname: Buelens
authors.affiliation: Max Planck Institute of Biochemistry, Project Group Biological Chemistry, Martinsried, Germany.
authors.forename: Sascha
authors.initials: S
authors.lastname: Serdjukow
authors.affiliation: Institute of Organic Chemistry and Chemical Biology, Buchmann Institute for Molecular Life Sciences, Cluster of Excellence "Macromolecular Complexes," Goethe University Frankfurt, Frankfurt am Main, Germany.
authors.forename: Melanie
authors.initials: M
authors.lastname: Janßen
authors.affiliation: Institute of Organic Chemistry and Chemical Biology, Buchmann Institute for Molecular Life Sciences, Cluster of Excellence "Macromolecular Complexes," Goethe University Frankfurt, Frankfurt am Main, Germany.
authors.forename: Niña
authors.initials: N
authors.lastname: Cortina
authors.affiliation: Max Planck Institute for Biophysical Chemistry, Department of Theoretical and Computational Biophysics, Göttingen, Germany.
authors.forename: Helmut
authors.initials: H
authors.lastname: Grubmüller
authors.affiliation: Institute of Organic Chemistry and Chemical Biology, Buchmann Institute for Molecular Life Sciences, Cluster of Excellence "Macromolecular Complexes," Goethe University Frankfurt, Frankfurt am Main, Germany.|Max Planck Institute of Biochemistry, Project Group Biological Chemistry, Martinsried, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Grininger
mesh_terms: D003352:Corynebacterium / Q000201:enzymology; D064429:Fatty Acid Synthases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D015394:Molecular Structure; D061065:Polyketides / Q000737:chemistry / Q000378:metabolism*; D015202:Protein Engineering
chemical_list: D061065:Polyketides; D064429:Fatty Acid Synthases