title: The Cell Death Triggered by the Nuclear Localized RxLR Effector PITG_22798 from Phytophthora infestans Is Suppressed by the Effector AVR3b.
abstract: Phytopathogenic oomycetes, such asPhytophthora infestans, potentially secrete many RxLR effector proteins into plant cells to modulate plant immune responses and promote colonization. However, the molecular mechanisms by which these RxLR effectors suppress plant immune responses are largely unknown. Here we describe an RxLR effector PITG_22798 (Gene accession: XM_002998349) that was upregulated during early infection of potato byP. infestans. By employment of agroinfiltration, we observed thatPITG_22798triggers cell death inNicotiana benthamiana. Confocal microscopic examination showed that PITG_22798-GFP (Green Fluorescent Protein) located in the host nucleus when expressed transiently inN. benthamianaleaves. A nuclear localization signal (NLS) domain of PITG_22798 is important for nuclear localization and cell death-inducing activity. Sequence alignment and transient expression showed thatPITG_22798from diverseP. infestansisolates are conserved, and transient expression ofPITG_22798enhancesP. infestanscolonization ofN. benthamianaleaves, which suggests thatPITG_22798contributes toP. infestansinfection.PITG_22798-triggered cell death is dependent on SGT1-mediated signaling and is suppressed by theP. infestansavirulence effector 3b (AVR3b). The present research provides a clue for further investigation of howP. infestanseffector PITG_22798 associates with and modulates host immunity.
authors.affiliation: Key Laboratory of Potato Biology and Biotechnology (Ministry of Agriculture, China), Huazhong Agricultural University, Wuhan 430070, China. hongyang8318@126.com.|School of Life Science, Yunnan Normal University, Kunming 650500, China. hongyang8318@126.com.
authors.forename: Hongyang
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Potato Biology and Biotechnology (Ministry of Agriculture, China), Huazhong Agricultural University, Wuhan 430070, China. 13349888011@163.com.
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Key Laboratory of Potato Biology and Biotechnology (Ministry of Agriculture, China), Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Potato Biology and Biotechnology (Ministry of Agriculture, China), Huazhong Agricultural University, Wuhan 430070, China. karenduren1985@gmail.com.
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.affiliation: Key Laboratory of Potato Biology and Biotechnology (Ministry of Agriculture, China), Huazhong Agricultural University, Wuhan 430070, China. houjuanok@163.com.
authors.forename: Juan
authors.initials: J
authors.lastname: Hou
authors.affiliation: Key Laboratory of Potato Biology and Biotechnology (Ministry of Agriculture, China), Huazhong Agricultural University, Wuhan 430070, China. jiangrui828@webmail.hzau.edu.cn.
authors.forename: Rui
authors.initials: R
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Potato Biology and Biotechnology (Ministry of Agriculture, China), Huazhong Agricultural University, Wuhan 430070, China. hongyang8318@126.com.
authors.forename: Haixia
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Potato Biology and Biotechnology (Ministry of Agriculture, China), Huazhong Agricultural University, Wuhan 430070, China. tianzhd@mail.hzau.edu.cn.
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Tian
authors.affiliation: Key Laboratory of Potato Biology and Biotechnology (Ministry of Agriculture, China), Huazhong Agricultural University, Wuhan 430070, China. xiech@mail.hzau.edu.cn.
authors.forename: Conghua
authors.initials: C
authors.lastname: Xie
mesh_terms: D016923:Cell Death; D002467:Cell Nucleus / Q000378:metabolism; D006790:Host-Parasite Interactions; D055750:Phytophthora infestans / Q000472:pathogenicity / Q000502:physiology*; D010944:Plants / Q000469:parasitology; D021381:Protein Transport; D015800:Protozoan Proteins / Q000378:metabolism*; D037521:Virulence Factors / Q000378:metabolism*
chemical_list: D015800:Protozoan Proteins; D037521:Virulence Factors
keywords: Nicotiana benthamiana; Phytophthora infestans; cell death; effector; plant immunity