title: Cultures of the Marine Bacterium Pseudovibrio denitrificans Ab134 Produce Bromotyrosine-Derived Alkaloids Previously Only Isolated from Marine Sponges.
abstract: Herein we report the isolation and spectroscopic identification of fistularin-3 (1), 11-hydroxyaerothionin (2), and verongidoic acid (3), as well as the UPLC-HRMS detection of aerothionin (4), homopurpuroceratic acid B (5), purealidin L (6), and aplysinamisine II (7), from cultures of the marine bacterium Pseudovibrio denitrificans Ab134, isolated from tissues of the marine sponge Arenosclera brasiliensis. These results unambiguously demonstrate for the first time that bromotyrosine-derived alkaloids that were previously isolated only from Verongida sponges can be biosynthesized by a marine bacterium.
authors.affiliation: Instituto de Química de São Carlos, Universidade de São Paulo , CP 780, CEP 13560-970, São Carlos, SP, Brazil.
authors.forename: Karen J
authors.initials: KJ
authors.lastname: Nicacio
authors.affiliation: Instituto de Química de São Carlos, Universidade de São Paulo , CP 780, CEP 13560-970, São Carlos, SP, Brazil.
authors.forename: Laura P
authors.initials: LP
authors.lastname: Ióca
authors.affiliation: Instituto de Biologia, Centro de Ciência da Saúde, Universidade Federal do Rio de Janeiro , Avenida Carlos Chagas Fo. 373, Bloco A, Anexo A3, Sl. 302, Cidade Universitária, CEP 21941-599, Rio de Janeiro, RJ, Brazil.
authors.forename: Adriana M
authors.initials: AM
authors.lastname: Fróes
authors.affiliation: Instituto de Biologia, Centro de Ciência da Saúde, Universidade Federal do Rio de Janeiro , Avenida Carlos Chagas Fo. 373, Bloco A, Anexo A3, Sl. 302, Cidade Universitária, CEP 21941-599, Rio de Janeiro, RJ, Brazil.
authors.forename: Luciana
authors.initials: L
authors.lastname: Leomil
authors.affiliation: Instituto de Biologia, Centro de Ciência da Saúde, Universidade Federal do Rio de Janeiro , Avenida Carlos Chagas Fo. 373, Bloco A, Anexo A3, Sl. 302, Cidade Universitária, CEP 21941-599, Rio de Janeiro, RJ, Brazil.
authors.forename: Luciana R
authors.initials: LR
authors.lastname: Appolinario
authors.affiliation: Instituto de Biologia, Centro de Ciência da Saúde, Universidade Federal do Rio de Janeiro , Avenida Carlos Chagas Fo. 373, Bloco A, Anexo A3, Sl. 302, Cidade Universitária, CEP 21941-599, Rio de Janeiro, RJ, Brazil.
authors.forename: Christiane C
authors.initials: CC
authors.lastname: Thompson
authors.affiliation: Instituto de Biologia, Centro de Ciência da Saúde, Universidade Federal do Rio de Janeiro , Avenida Carlos Chagas Fo. 373, Bloco A, Anexo A3, Sl. 302, Cidade Universitária, CEP 21941-599, Rio de Janeiro, RJ, Brazil.
authors.forename: Fabiano L
authors.initials: FL
authors.lastname: Thompson
authors.affiliation: Departamento de Química, Universidade Federal de São Carlos , CEP 13565-905, São Carlos, SP, Brazil.
authors.forename: Antonio G
authors.initials: AG
authors.lastname: Ferreira
authors.affiliation: Departments of Chemistry and Earth, Ocean & Atmospheric Sciences, University of British Columbia , Vancouver, BC V6T 1Z1, Canada.
authors.forename: David E
authors.initials: DE
authors.lastname: Williams
authors.affiliation: Departments of Chemistry and Earth, Ocean & Atmospheric Sciences, University of British Columbia , Vancouver, BC V6T 1Z1, Canada.
authors.forename: Raymond J
authors.identifier: 0000-0002-7607-8213
authors.initials: RJ
authors.lastname: Andersen
authors.affiliation: College of Pharmacy, Department of Medicinal Chemistry and Pharmacognosy, Center for Biomolecular Sciences, University of Illinois at Chicago , 900 S. Ashland Avenue, Chicago, Illinois 60607, United States.
authors.forename: Alessandra S
authors.initials: AS
authors.lastname: Eustaquio
authors.affiliation: Instituto de Química de São Carlos, Universidade de São Paulo , CP 780, CEP 13560-970, São Carlos, SP, Brazil.
authors.forename: Roberto G S
authors.identifier: 0000-0003-0118-2523
authors.initials: RG
authors.lastname: Berlinck
mesh_terms: D000470:Alkaloids / Q000737:chemistry*; D000818:Animals; D001419:Bacteria / Q000737:chemistry*; D001938:Brazil; D007555:Isoxazoles / Q000737:chemistry; D008386:Marine Biology; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D009792:Oceans and Seas; D011161:Porifera / Q000737:chemistry*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D000470:Alkaloids; D007555:Isoxazoles; C475724:bromotyrosine; D014443:Tyrosine; C076216:fistularin 3