title: Possible Involvement of F1F0-ATP synthase and Intracellular ATP in Keratinocyte Differentiation in normal skin and skin lesions.
abstract: The F1F0-ATP synthase, an enzyme complex, is mainly located on the mitochondrial inner membrane or sometimes cytomembrane to generate or hydrolyze ATP, play a role in cell proliferation. This study focused on the role of F1F0-ATP synthase in keratinocyte differentiation, and its relationship with intracellular and extracellular ATP (InATP and ExATP). The F1F0-ATP synthase β subunit (ATP5B) expression in various skin tissues and confluence-dependent HaCaT differentiation models was detected. ATP5B expression increased with keratinocyte and HaCaT cell differentiation in normal skin, some epidermis hyper-proliferative diseases, squamous cell carcinoma, and the HaCaT cell differentiation model. The impact of InATP and ExATP content on HaCaT differentiation was reflected by the expression of the differentiation marker involucrin. Inhibition of F1F0-ATP synthase blocked HaCaT cell differentiation, which was associated with a decrease of InATP content, but not with changes of ExATP. Our results revealed that F1F0-ATP synthase expression is associated with the process of keratinocyte differentiation which may possibly be related to InATP synthesis.
authors.affiliation: Department of Dermatology, XiangYa Hospital, Central South University, Changsha, China.|Department of Rheumatology and Immunology, XiangYa Hospital, Central South University, Changsha, China.
authors.forename: Xie
authors.initials: X
authors.lastname: Xiaoyun
authors.affiliation: Department of Plastic and Reconstructive Surgery, The Third XiangYa Hospital, Central South University, Changsha, China.
authors.forename: Han
authors.initials: H
authors.lastname: Chaofei
authors.affiliation: Department of Dermatology, XiangYa Hospital, Central South University, Changsha, China.|Hunan Key Laboratory of Skin Cancer and Psoriasis, XiangYa Hospital, Central South University, Changsha, China.
authors.forename: Zeng
authors.initials: Z
authors.lastname: Weiqi
authors.affiliation: Department of Dermatology, XiangYa Hospital, Central South University, Changsha, China.|Department of Nephrology, XiangYa Hospital, Central South University, Changsha, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Dermatology, XiangYa Hospital, Central South University, Changsha, China.|Hunan Key Laboratory of Skin Cancer and Psoriasis, XiangYa Hospital, Central South University, Changsha, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Lixia
authors.affiliation: Department of Dermatology, XiangYa Hospital, Central South University, Changsha, China.|Hunan Key Laboratory of Skin Cancer and Psoriasis, XiangYa Hospital, Central South University, Changsha, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Queping
authors.affiliation: Department of Dermatology, XiangYa Hospital, Central South University, Changsha, China.|Hunan Key Laboratory of Skin Cancer and Psoriasis, XiangYa Hospital, Central South University, Changsha, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Cong
authors.affiliation: Department of Dermatology, XiangYa Hospital, Central South University, Changsha, China.|Hunan Key Laboratory of Skin Cancer and Psoriasis, XiangYa Hospital, Central South University, Changsha, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Shuang
authors.affiliation: Department of Dermatology, XiangYa Hospital, Central South University, Changsha, China.|Hunan Key Laboratory of Skin Cancer and Psoriasis, XiangYa Hospital, Central South University, Changsha, China.
authors.forename: Su
authors.initials: S
authors.lastname: Juan
authors.affiliation: Department of Dermatology, XiangYa Hospital, Central South University, Changsha, China.|Hunan Key Laboratory of Skin Cancer and Psoriasis, XiangYa Hospital, Central South University, Changsha, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Xiang
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002454:Cell Differentiation; D002461:Cell Line, Transformed; D003872:Dermatitis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D007636:Keratoacanthoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D017492:Keratosis, Seborrheic / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008928:Mitochondria / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000378:metabolism*; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics* / Q000378:metabolism; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism; D011536:Prurigo / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D011565:Psoriasis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D012867:Skin / Q000166:cytology / Q000378:metabolism; D012878:Skin Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D014860:Warts / Q000235:genetics / Q000378:metabolism / Q000473:pathology
chemical_list: C506526:ATP5F1B protein, human; D011498:Protein Precursors; C032253:involucrin; D000255:Adenosine Triphosphate; D025261:Mitochondrial Proton-Translocating ATPases