title: Hydrophobic-carbon-dot-based dual-emission micelle for ratiometric fluorescence biosensing and imaging of Cu2+in liver cells.
abstract: Copper is closely related to liver damage, therefore, it is essential to develop a simple and sensitive strategy to detect copper ions (Cu2+) in liver cells. A hydrophobic carbon dots (HCDs)-based dual-emission fluorescent probe for Cu2+was prepared by encapsulating HCDs in micelles formed by self-assembly of amphiphilic polymer DSPE-PEG and tetrakis (4-carboxyphenyl) porphyrin (TCPP)-modified DSPE-PEG. The obtained probe showed characteristic fluorescence emissions of HCDs and TCPP with large emission shift of 170nm with single-wavelength excitation. In the presence of Cu2+, the fluorescence of TCPP was quenched and that of HCDs remained unchanged, displaying ratiometric fluorescence response to Cu2+. The developed probe exhibited high sensitivity (detection limit down to 36nM) and selectivity to Cu2+over other substances, and the probe was used to image the changes of Cu2+level in liver cells successfully.
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Lu
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China.
authors.forename: Chongchong
authors.initials: C
authors.lastname: Feng
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China.
authors.forename: Fengyang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Center of Pharmaceutics, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, PR China.
authors.forename: Haijun
authors.initials: H
authors.lastname: Yu
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China. Electronic address: zaxu@chem.ecnu.edu.cn.
authors.forename: Zhiai
authors.initials: Z
authors.lastname: Xu
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D002413:Cations, Divalent / Q000032:analysis; D003300:Copper / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D057230:Limit of Detection; D008099:Liver / Q000737:chemistry / Q000166:cytology; D008823:Micelles; D061848:Optical Imaging / Q000379:methods*; D010714:Phosphatidylethanolamines / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011166:Porphyrins / Q000737:chemistry; D045663:Quantum Dots / Q000737:chemistry* / Q000648:ultrastructure; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D002413:Cations, Divalent; D005456:Fluorescent Dyes; D008823:Micelles; D010714:Phosphatidylethanolamines; D011166:Porphyrins; C018395:tetracarboxyphenylporphine; C038089:1,2-distearoylphosphatidylethanolamine; D011092:Polyethylene Glycols; D002244:Carbon; D003300:Copper
keywords: Copper ion; Fluorescence; Hydrophobic carbon dots; Micelle; Ratiometric; TCPP