title: S100A7 promotes the migration, invasion and metastasis of human cervical cancer cells through epithelial-mesenchymal transition.
abstract: S100A7 is an EF-hand calcium-binding protein that has been suggested to be implicated in cell proliferation, migration, invasion and tumor metastasis. However, its role in cervical cancer has not yet been fully clarified. The present study used immunohistochemistry analysis of S100A7 in clinical specimens of cervical cancer to show that S100A7 expression was significantly upregulated in cervical cancer tissues compared with normal cervical tissues and S100A7 expression in high grade cervical intraepithelial neoplasm (CIN) was significantly higher than cervical cancer. Statistical analysis showed that S100A7 expression was associated with tumor grade (P <0.01) and lymph node metastasis (P <0.05). Functional studies showed that overexpression of S100A7 in cervical cancer cells promoted migration, invasion and metastasis of cervical cancer cells without influencing cell proliferation. Furthermore, S100A7 was found to be secreted into the conditioned media and extracellular S100A7 enhanced cell migration and invasion. Mechanistically, S100A7 bound to RAGE and activated ERK signaling pathway. And S100A7 enhanced cell mesenchymal properties and induced epithelial-mesenchymal transition. In summary, these data reveal a crucial role for S100A7 in regulating cell migration, invasion, metastasis and EMT of cervical cancer and suggest that targeting S100A7 may offer a new targeted strategy for cervical cancer.
authors.affiliation: Department of Obstetrics and Gynecology, The Affiliated Hospital of Qingdao University, Qingdao 266061, People's Republic of China.
authors.forename: Tian
authors.initials: T
authors.lastname: Tian
authors.affiliation: State Key Lab of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, People's Republic of China.
authors.forename: Xukun
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, The Affiliated Hospital of Qingdao University, Qingdao 266061, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Hua
authors.affiliation: State Key Lab of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, People's Republic of China.
authors.forename: Jianlin
authors.initials: J
authors.lastname: Ma
authors.affiliation: State Key Lab of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, People's Republic of China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Wu
authors.affiliation: State Key Lab of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, People's Republic of China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Lab of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, People's Republic of China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Obstetrics and Gynecology, The Affiliated Hospital of Qingdao University, Qingdao 266061, People's Republic of China.
authors.forename: Zhumei
authors.initials: Z
authors.lastname: Cui
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D000075023:S100 Calcium Binding Protein A7 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D002578:Uterine Cervical Dysplasia / Q000235:genetics / Q000378:metabolism / Q000473:pathology
chemical_list: D000075023:S100 Calcium Binding Protein A7; C089242:S100A7 protein, human
keywords: EMT; S100A7; cervical cancer; invasion; metastasis