title: Synthesis of lacto-N-tetraose.
abstract: Human milk oligosaccharides (HMOs) are the third largest macromolecular component of breast milk and offer infants numerous health benefits, most of which stem from the development of a healthy microbiome. Characterization, quantification, and chemical derivatization of HMOs remains a frontier issue in glycobiology due to the challenge of isolating appreciable quantities of homogenous HMOs from breast milk. Herein, we report the synthesis of the human milk tetrasaccharide lacto-N-tetraose (LNT). LNT is ubiquitous in human breast milk as it is a core structure common to longer-chain HMOs and many glycolipids.
authors.affiliation: Department of Chemistry, Institute of Chemical Biology, Vanderbilt University, Nashville, TN, 37235, United States.
authors.forename: Kelly M
authors.initials: KM
authors.lastname: Craft
authors.affiliation: Department of Chemistry, Institute of Chemical Biology, Vanderbilt University, Nashville, TN, 37235, United States. Electronic address: steven.d.townsend@vanderbilt.edu.
authors.forename: Steven D
authors.initials: SD
authors.lastname: Townsend
mesh_terms: D000081:Acetamides / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005260:Female; D054794:Glycomics; D006801:Humans; D008895:Milk, Human / Q000737:chemistry*; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000302:isolation & purification; D011714:Pyrans / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000081:Acetamides; D009844:Oligosaccharides; D011714:Pyrans; C013084:lacto-N-neotetraose