title: Sr-substituted bone cements direct mesenchymal stem cells, osteoblasts and osteoclasts fate.
abstract: Strontium-substituted apatitic bone cements enriched with sodium alginate were developed as a potential modulator of bone cells fate. The biological impact of the bone cement were investigated in vitro through the study of the effect of the nanostructured apatitic composition and the doping of strontium on mesenchymal stem cells, pre-osteoblasts and osteoclasts behaviours. Up to 14 days of culture the bone cells viability, proliferation, morphology and gene expression profiles were evaluated. The results showed that different concentrations of strontium were able to evoke a cell-specific response, in fact an inductive effect on mesenchymal stem cells differentiation and pre-osteoblasts proliferation and an inhibitory effect on osteoclasts activity were observed. Moreover, the apatitic structure of the cements provided a biomimetic environment suitable for bone cells growth. Therefore, the combination of biological features of this bone cement makes it as promising biomaterials for tissue regeneration.
authors.affiliation: Institute of Science and Technology for Ceramics, National Research Council, Faenza, Ravenna, Italy.
authors.forename: Monica
authors.identifier: http://orcid.org/0000-0002-9192-8554
authors.initials: M
authors.lastname: Montesi
authors.affiliation: Institute of Science and Technology for Ceramics, National Research Council, Faenza, Ravenna, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Panseri
authors.affiliation: Institute of Science and Technology for Ceramics, National Research Council, Faenza, Ravenna, Italy.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Dapporto
authors.affiliation: Institute of Science and Technology for Ceramics, National Research Council, Faenza, Ravenna, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Tampieri
authors.affiliation: Institute of Science and Technology for Ceramics, National Research Council, Faenza, Ravenna, Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Sprio
mesh_terms: D000818:Animals; D001843:Bone Cements / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D010010:Osteoclasts / Q000378:metabolism*; D013324:Strontium / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D001843:Bone Cements; D013324:Strontium