title: Perinatal administration of bisphenol A alters the expression of tight junction proteins in the uterus and reduces the implantation rate.
abstract: We studied the effect of bisphenol-A (BPA) administration to rats, during the perinatal period, on the fertility of F1 generation and on the expression of tight junction (TJ) proteins in the uterus during early pregnancy. Pregnant Wistar dams (F0) received: BPA-L (0.05mg/kg/day), BPA-H (20mg/kg/day) or vehicle, from gestational day (GD) 6 to lactation day 21. F1 female pups were mated at 3 months of age and sacrificed at GD 1, 3, 6, and 7. Serum hormonal levels, ovulation rate, number of implantation sites and expression of TJ proteins in the uterus of F1 females were evaluated. BPA treatment induced no change in ovulation rate, but induced alterations in progesterone (P4) and estradiol (E2) serum levels, and in implantation rate. With regards to TJ proteins, BPA-H increased claudin-1 during all GDs; eliminated the peaks of claudins -3 and -4 at GD 3 and 6, respectively; and decreased claudin-7 at GD 6, ZO-1 from GD 1-6, and claudin-3 at GD 7 in stromal cells. BPA-L instead, eliminated claudin-3 peak at GD 3, increased claudin-4 and decreased claudin-7 from GD 1-6, decreased claudin-1 at GD 3 and 7 and claudin-4 at GD 7 in stromal cells. BPA-L also decreased ZO-1 at GDs 1 and 3 and increased ZO-1 at GD 6. Thus, BPA treatment during perinatal period perturbed, when the animals reached adulthood and became pregnant, the particular expression of TJ proteins in the uterine epithelium and reduced in consequence the number of implantation sites.
authors.affiliation: Facultad de Química, Departamento de Biología, Universidad Nacional Autónoma de Mexico, Mexico D.F. 04510, Mexico.
authors.forename: Annia A
authors.initials: AA
authors.lastname: Martínez-Peña
authors.affiliation: Facultad de Química, Departamento de Biología, Universidad Nacional Autónoma de Mexico, Mexico D.F. 04510, Mexico.
authors.forename: Jorge
authors.initials: J
authors.lastname: Rivera-Baños
authors.affiliation: Facultad de Química, Departamento de Biología, Universidad Nacional Autónoma de Mexico, Mexico D.F. 04510, Mexico.
authors.forename: Laura L
authors.initials: LL
authors.lastname: Méndez-Carrillo
authors.affiliation: Facultad de Química, Departamento de Biología, Universidad Nacional Autónoma de Mexico, Mexico D.F. 04510, Mexico.
authors.forename: Marcos I
authors.initials: MI
authors.lastname: Ramírez-Solano
authors.affiliation: Facultad de Química, Departamento de Biología, Universidad Nacional Autónoma de Mexico, Mexico D.F. 04510, Mexico.
authors.forename: Aarón
authors.initials: A
authors.lastname: Galindo-Bustamante
authors.affiliation: Facultad de Química, Departamento de Biología, Universidad Nacional Autónoma de Mexico, Mexico D.F. 04510, Mexico.
authors.forename: J Carlos
authors.initials: JC
authors.lastname: Páez-Franco
authors.affiliation: Instituto Nacional de Ciencias Médicas y de la Nutrición Salvador Zubirán, Mexico, D.F. 14080, Mexico.
authors.forename: Sumiko
authors.initials: S
authors.lastname: Morimoto
authors.affiliation: Centro de Investigación y Estudios Avanzados (CINVESTAV), Departamento de Fisiología, Biofísica y Neurociencias, Mexico, D.F. 07360, Mexico.
authors.forename: Lorenza
authors.initials: L
authors.lastname: González-Mariscal
authors.affiliation: Facultad de Estudios Superiores Zaragoza, Laboratorio de Neuroendocrinología, Universidad Nacional Autónoma de Mexico, Mexico, D.F. 15000, Mexico.
authors.forename: M Esther
authors.initials: ME
authors.lastname: Cruz
authors.affiliation: Facultad de Química, Departamento de Biología, Universidad Nacional Autónoma de Mexico, Mexico D.F. 04510, Mexico. Electronic address: adrimed@yahoo.com.
authors.forename: C Adriana
authors.initials: CA
authors.lastname: Mendoza-Rodríguez
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D010064:Embryo Implantation / Q000187:drug effects; D052244:Endocrine Disruptors / Q000633:toxicity*; D004958:Estradiol / Q000097:blood; D005260:Female; D007774:Lactation; D008297:Male; D010636:Phenols / Q000633:toxicity*; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D017208:Rats, Wistar; D062725:Tight Junction Proteins / Q000378:metabolism*; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D004958:Estradiol; D010636:Phenols; D011374:Progesterone; D062725:Tight Junction Proteins; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol-A; Claudins; Implantation; Tight junctions; ZO-1