title: Ectopic Expression of the Wild Grape WRKY Transcription Factor VqWRKY52 inArabidopsis thalianaEnhances Resistance to the Biotrophic Pathogen Powdery Mildew But Not to the Necrotrophic PathogenBotrytis cinerea.
abstract: WRKY transcription factors are known to play important roles in plant responses to biotic stresses. We previously showed that the expression of the WRKY gene,VqWRKY52, from Chinese wildVitis quinquangulariswas strongly induced 24 h post inoculation with powdery mildew. In this study, we analyzed the expression levels ofVqWRKY52following treatment with the defense related hormones salicylic acid (SA) and methyl jasmonate, revealing thatVqWRKY52was strongly induced by SA but not JA. We characterized theVqWRKY52gene, which encodes a WRKY III gene family member, and found that ectopic expression inArabidopsis thalianaenhanced resistance to powdery mildew andPseudomonas syringaepv.tomatoDC3000, but increased susceptibility toBotrytis cinerea, compared with wild type (WT) plants. The transgenicA. thalianalines displayed strong cell death induced by the biotrophic powdery mildew pathogen, the hemibiotrophicP. syringepathogen and the necrotrophic pathogenB. cinerea. In addition, the relative expression levels of various defense-related genes were compared between the transgenicA. thalianalines and WT plants following the infection by different pathogens. Collectively, the results indicated thatVqWRKY52plays essential roles in the SA dependent signal transduction pathway and that it can enhance the hypersensitive response cell death triggered by microbial pathogens.
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F UniversityYangling, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F UniversityYangling, China.
authors.forename: Xianhang
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F UniversityYangling, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F UniversityYangling, China.
authors.forename: Rongrong
authors.initials: R
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F UniversityYangling, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F UniversityYangling, China.
authors.forename: Mingxing
authors.initials: M
authors.lastname: Tu
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F UniversityYangling, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F UniversityYangling, China.
authors.forename: Dejun
authors.initials: D
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F UniversityYangling, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F UniversityYangling, China.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F UniversityYangling, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F UniversityYangling, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Wan
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F UniversityYangling, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F UniversityYangling, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F UniversityYangling, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F UniversityYangling, China.
authors.forename: Xiping
authors.initials: X
authors.lastname: Wang
keywords: Chinese wild Vitis; WRKY; cell death; hypersensitive response; reactive oxygen species; salicylic acid