title: Oral administration of Nigella sativa oil ameliorates the effect of cisplatin on brush border membrane enzymes, carbohydrate metabolism and antioxidant system in rat intestine.
abstract: Cisplatin (CP) is an effective chemotherapeutic agent that induces gastrointestinal toxicity. Nigella sativa oil (NSO) has been shown to be beneficial in a wide range of gastrointestinal disorders. The present study investigates the possible protective effect of NSO on CP-induced gastrointestinal toxicity. NSO administration (2ml/kg bwt, orally), prior to and following, a single dose CP treatment (6mg/kg bwt. ip), significantly attenuated the CP-induced decrease in brush border membrane (BBM) enzyme activities in intestinal homogenates and BBM vesicles (BBMV). NSO administration also mitigated CP induced alterations in the activities of carbohydrate metabolism enzymes and in the enzymatic and non-enzymatic antioxidant parameters in the intestine. The results suggest that NSO by empowering the endogenous antioxidant system improves intestinal redox and metabolic status and restores BBM integrity in CP treated rats. Histopathological studies supported the biochemical findings. Thus, NSO may help prevent the accompanying gastrointestinal dysfunction in CP chemotherapy.
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India.
authors.forename: Faaiza
authors.initials: F
authors.lastname: Shahid
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India.
authors.forename: Zeba
authors.initials: Z
authors.lastname: Farooqui
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India.
authors.forename: Sana
authors.initials: S
authors.lastname: Rizwan
authors.affiliation: Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India.
authors.forename: Subuhi
authors.initials: S
authors.lastname: Abidi
authors.affiliation: Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India.
authors.forename: Iqbal
authors.initials: I
authors.lastname: Parwez
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India. Electronic address: dr.farahkhan23@gmail.com.
authors.forename: Farah
authors.initials: F
authors.lastname: Khan
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002945:Cisplatin / Q000633:toxicity*; D007422:Intestines / Q000187:drug effects*; D008297:Male; D008871:Microvilli / Q000187:drug effects; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010938:Plant Oils / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D029974:Carum
chemical_list: D000970:Antineoplastic Agents; D010938:Plant Oils; C104525:caraway oil; D002945:Cisplatin
keywords: Antioxidant system; Carbohydrate metabolism; Cisplatin; Gastrointestinal toxicity; Nigella sativa oil