title: Molecular pathways underlying inhibitory effect of antimicrobial peptide Nal-P-113 on bacteria biofilms formation of Porphyromonas gingivalis W83 by DNA microarray.
abstract: BACKGROUND
  Wound-related infection remains a major challenge for health professionals. One disadvantage in conventional antibiotics is their inability to penetrate biofilms, the main protective strategy for bacteria to evade irradiation. Previously, we have shown that synthetic antimicrobial peptides could inhibit bacterial biofilms formation.
  
  
  RESULTS
  In this study, we first delineated how Nal-P-113, a novel antimicrobial peptide, exerted its inhibitory effects on Porphyromonas gingivalis W83 biofilms formation at a low concentration. Secondly, we performed gene expression profiling and validated that Nal-P-113 at a low dose significantly down-regulated genes related to mobile and extrachromosomal element functions, transport and binding proteins in Porphyromonas gingivalis W83.
  
  
  CONCLUSIONS
  These findings suggest that Nal-P-113 at low dose is sufficient to inhibit the formation of biofilms although Porphyromonas gingivalis W83 may maintain its survival in the oral cavity. The newly discovered molecular pathways may add the knowledge of developing a new strategy to target bacterial infections in combination with current first-line treatment in periodontitis.
authors.affiliation: Department of Periodontics, School of Stomatology, China Medical University, Shenyang, 110002, China.
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Wang
authors.affiliation: Department of Periodontics, School of Stomatology, China Medical University, Shenyang, 110002, China.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Periodontics, School of Stomatology, China Medical University, Shenyang, 110002, China.
authors.forename: Li-Si
authors.initials: LS
authors.lastname: Tan
authors.affiliation: Institute of Biotechnology and Department of Medical Science, National Tsing Hua University, Hsinchu, 300, Taiwan.
authors.forename: Hui-Yuan
authors.initials: HY
authors.lastname: Yu
authors.affiliation: Institute of Biotechnology and Department of Medical Science, National Tsing Hua University, Hsinchu, 300, Taiwan.
authors.forename: Jya-Wei
authors.initials: JW
authors.lastname: Cheng
authors.affiliation: Department of Periodontics, School of Stomatology, China Medical University, Shenyang, 110002, China. yppan@cmu.edu.cn.
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Pan
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D001426:Bacterial Proteins; D018441:Biofilms / Q000187:drug effects*; D002352:Carrier Proteins; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D016695:Glycosyltransferases / Q000037:antagonists & inhibitors*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D009055:Mouth / Q000382:microbiology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010518:Periodontitis / Q000188:drug therapy / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D006861:Hydrogen Peroxide; D016695:Glycosyltransferases; C441626:peptide P
keywords: Biofilms; Microarray; Nal-P-113; Porphyromonas gingivalis W83