title: Mass spectrometry captures structural intermediates in protein fiber self-assembly.
abstract: Self-assembling proteins, the basis for a broad range of biological scaffolds, are challenging to study using most structural biology approaches. Here we show that mass spectrometry (MS) in combination with MD simulations captures structural features of short-lived oligomeric intermediates in spider silk formation, providing direct insights into its complex assembly process.
authors.affiliation: Department of Chemistry, Physical & Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, OX1 3QZ, UK. Carol.Robinson@chem.ox.ac.uk.
authors.forename: Michael
authors.initials: M
authors.lastname: Landreh
authors.affiliation: Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors.forename: Marlene
authors.initials: M
authors.lastname: Andersson
authors.affiliation: Department of Chemistry - BMC, Uppsala University, Box 576, SE-751 23, Uppsala, Sweden.
authors.forename: Erik G
authors.initials: EG
authors.lastname: Marklund
authors.affiliation: Institute of Biological Sciences and Biotechnology, Donghua University, Shanghai, 201620, P. R. China.
authors.forename: Qiupin
authors.initials: Q
authors.lastname: Jia
authors.affiliation: Institute of Biological Sciences and Biotechnology, Donghua University, Shanghai, 201620, P. R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, Uppsala, Sweden and Division of Neurogeriatrics, Department of Neurobiology, Care Sciences and Society (NVS), Center for Alzheimer Research, Karolinska Institutet, Huddinge, 14157, Stockholm, Sweden. Janne.Johansson@ki.se Anna.Rising@ki.se.
authors.forename: Jan
authors.initials: J
authors.lastname: Johansson
authors.affiliation: Department of Chemistry, Physical & Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, OX1 3QZ, UK. Carol.Robinson@chem.ox.ac.uk.
authors.forename: Carol V
authors.identifier: http://orcid.org/0000-0002-7958-4074
authors.initials: CV
authors.lastname: Robinson
authors.affiliation: Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, Uppsala, Sweden and Division of Neurogeriatrics, Department of Neurobiology, Care Sciences and Society (NVS), Center for Alzheimer Research, Karolinska Institutet, Huddinge, 14157, Stockholm, Sweden. Janne.Johansson@ki.se Anna.Rising@ki.se.
authors.forename: Anna
authors.initials: A
authors.lastname: Rising
mesh_terms: D000818:Animals; D001097:Arachnida / Q000737:chemistry; D060829:Arthropod Proteins / Q000737:chemistry*; D005349:Fibroins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry; D056004:Molecular Dynamics Simulation; D010446:Peptide Fragments / Q000737:chemistry*; D011487:Protein Conformation; D000072417:Protein Domains; D055503:Protein Multimerization; D058767:Protein Unfolding
chemical_list: D060829:Arthropod Proteins; D010446:Peptide Fragments; D005349:Fibroins