title: Selenium Deficiency Induced Injury in Chicken Muscular Stomach by Downregulating Selenoproteins.
abstract: The aim of the present study was to examine the effect of selenium (Se) deficiency on the expression of selenoproteins in chicken muscular stomach and to detect the correlation of selenoproteins with muscular stomach injuries. One-day-old broiler chickens were maintained for 55 days on a normal diet (0.2 mg/kg) or a Se-deficient diet (0.033 mg Se/kg). The expression levels of 25 selenoproteins, heat shock proteins (HSPs), and inflammatory factors were then examined by real-time PCR. Following this, the correlation between selenoproteins, HSPs, and inflammatory factors was analyzed by principal component analysis (PCA). The results showed that Se deficiency decreased the expression of 25 selenoproteins (P < 0.05), but increased the expression of HSP27, HSP40, HSP60, HSP70, and HSP90, and NF-κB, iNOS, TNF-α, COX-2, and HO-1 (P < 0.05). Selenoproteins showed a high negative correlation with HSPs and inflammatory factors. Thus, the results suggested that Se deficiency induced muscular stomach injuries by decreasing the expression of selenoproteins. In addition, selenoproteins play an important role in regulating HSPs and inflammatory response. The muscular stomach is a key target of Se deficiency and may play a special role in response to Se deficiency.
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin, 150030, China.|College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Huang
authors.affiliation: Heilongjiang Bayi Agricultural University, Daqing, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Sun
authors.affiliation: Heilongjiang Polytechnic, Harbin, 150080, China.
authors.forename: Jiuli
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Heilongjiang Polytechnic, Harbin, 150080, China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Gao
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: Guangxing
authors.initials: G
authors.lastname: Li
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin, 150030, China. changying@neau.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chang
mesh_terms: D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002645:Chickens / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects; D005786:Gene Expression Regulation; D005895:Gizzard, Avian / Q000378:metabolism* / Q000503:physiopathology; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism / Q000662:veterinary; D025341:Principal Component Analysis; D012643:Selenium / Q000172:deficiency*; D051140:Selenoproteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D006360:Heat-Shock Proteins; D051140:Selenoproteins; D012643:Selenium
keywords: Heat shock protein; Inflammation factors; Muscular stomach; Selenium deficiency; Selenoprotein