title: Construction of AIEgens-Based Bioprobe with Two Fluorescent Signals for Enhanced Monitor of Extracellular and Intracellular Telomerase Activity.
abstract: Detections of telomerase activity in vitro and in living cells are of great importance for clinical diagnosis of cancer. In this work, an AIEgens-based bioprobe with two fluorescent signals for enhanced monitor of extracellular and intracellular telomerase activity is designed. After addition of telomerase, two positively charged AIEgens (Silole-R and TPE-H) bind to quencher group labeled primer (QP) and the extension repeated units, leading enhancement of two telomerase-triggered fluorescent signals. Furthermore, by combination the wider linear range in vitro and lower background in living cells imaging, the bioprobe is used to detect telomerase extracted from various cell lines (MCF-7, HeLa, E-J, and HLF), 50 bladder cancer patients' urine samples, 10 normal people's urine samples, and also applied in mapping telomerase activity inside living cells (MCF-7, HeLa, MDA-MB-231, and HT1080). The results show that this well-designed strategy can successfully detect telomerase activity in vitro and in living cells with high sensitivity, indicating the potential application of this method in cancer cells bioimaging and clinical cancer diagnosis.
authors.affiliation: Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, People's Republic of China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhuang
authors.affiliation: Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, People's Republic of China.
authors.forename: Chunli
authors.initials: C
authors.lastname: Shang
authors.affiliation: Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, People's Republic of China.
authors.forename: Xiaoding
authors.initials: X
authors.lastname: Lou
authors.affiliation: Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, People's Republic of China.|Shenzhen Institute of Huazhong University of Science and Technology, Shenzhen 518000, People's Republic of China.
authors.forename: Fan
authors.identifier: 0000-0001-7705-4638
authors.initials: F
authors.lastname: Xia
mesh_terms: D015374:Biosensing Techniques; D045744:Cell Line, Tumor; D005189:False Positive Reactions; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D057054:Molecular Imaging / Q000379:methods; D012821:Silanes / Q000737:chemistry*; D013379:Substrate Specificity; D019098:Telomerase / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000201:enzymology
chemical_list: D005456:Fluorescent Dyes; D012821:Silanes; C540376:silole; D019098:Telomerase