title: Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga.
abstract: Theraphosidae spider venoms are well known for possess a complex mixture of protein and non-protein compounds in their venom. The objective of this study was to report and identify different proteins translated from the venom gland DNA information of the recently described Theraphosidae spider Pamphobeteus verdolaga. Using a venom gland transcriptomic analysis, we reported a set of the first complete sequences of seven different proteins of the recenlty described Theraphosidae spider P. verdolaga. Protein analysis indicates the presence of different proteins on the venom composition of this new spider, some of them uncommon in the Theraphosidae family. MS/MS analysis of P. verdolaga showed different fragments matching sphingomyelinases (sicaritoxin), barytoxins, hexatoxins, latroinsectotoxins, and linear (zadotoxins) peptides. Only four of the MS/MS fragments showed 100% sequence similarity with one of the transcribed proteins. Transcriptomic analysis showed the presence of different groups of proteins like phospholipases, hyaluronidases, inhibitory cysteine knots (ICK) peptides among others. The three database of protein domains used in this study (Pfam, SMART and CDD) showed congruency in the search of unique conserved protein domain for only four of the translated proteins. Those proteins matched with EF-hand proteins, cysteine rich secretory proteins, jingzhaotoxins, theraphotoxins and hexatoxins, from different Mygalomorphae spiders belonging to the families Theraphosidae, Barychelidae and Hexathelidae. None of the analyzed sequences showed a complete 100% similarity.
authors.affiliation: Programa de Ofidismo/Escorpionismo - Serpentario, Universidad de Antioquia UdeA, Carrera 53 No 61-30, Medellín, 050010, Antioquia, Colombia; Facultad de Ciencias Farmacéuticas y Alimentarias, Universidad de Antioquia UdeA, calle 70 No 52-21, Medellín, 050010, Antioquia, Colombia. Electronic address: sebastian.estrada@udea.edu.co.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Estrada-Gómez
authors.affiliation: Facultad de Medicina, Universidad Cooperativa de Colombia, Calle 50 A No 41-20, Medellín, 050012, Antioquia, Colombia. Electronic address: leidy.vargasmu@campusucc.edu.co.
authors.forename: Leidy Johana
authors.initials: LJ
authors.lastname: Vargas-Muñoz
authors.affiliation: Departamento de Ciencias, Universidad Iberoamericana de Ciencias y Tecnología, Padre Miguel de Olivares, 1620, Santiago, Chile; Universidad Bernardo O'Higgins, Centro de Investigación en Recursos Naturales y Sustentabilidad, Fábrica, 1990, Segundo Piso, Santiago, Chile. Electronic address: monica.saldarriaga@uibero.cl.
authors.forename: Mónica
authors.initials: M
authors.lastname: Saldarriaga-Córdoba
authors.affiliation: Laboratório Especial de Ecologia e Evolução, Instituto Butantan, Avenida Vital Brazil 1500, 05503-900, São Paulo, SP, Brazil; Programa de Pós-Graduação em Biologia Animal, Universidade Estadual Paulista "Júlio de Mesquita Filho", São José do Rio Preto, SP, 15054-000, Brazil. Electronic address: yelicigi@gmail.com.
authors.forename: Yeimy
authors.initials: Y
authors.lastname: Cifuentes
authors.affiliation: Sección Entomología, Facultad de Ciencias, Universidad de La República, Iguá 4225, 11400, Montevideo, Uruguay. Electronic address: caperafanl@gmail.com.
authors.forename: Carlos
authors.initials: C
authors.lastname: Perafan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019295:Computational Biology; D020869:Gene Expression Profiling; D010455:Peptides / Q000737:chemistry; D011487:Protein Conformation; D040901:Proteomics; D016415:Sequence Alignment; D013111:Spider Venoms / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D013112:Spiders / Q000737:chemistry* / Q000145:classification / Q000235:genetics*; D053719:Tandem Mass Spectrometry
chemical_list: D010455:Peptides; D013111:Spider Venoms
keywords: Pamphobeteus verdolaga; Proteomic; Theraphosidae; Transcriptomic