title: A tree shrew glioblastoma model recapitulates features of human glioblastoma.
abstract: Tupaia belangeri (tree shrew), an animal species whose genome has significantly higher similarity to primates than rodents, has been used in biomedical research. To generate animal models that reproduce the human tumors more faithfully than rodents, we present the first report of a cancer model mimicking human tumor genetics in tree shrew. By engineering a lentiviral system for the transduction of mutant H-Ras and a shRNA against tree shrew p53, we successfully generated malignant glioma in tree shrew. The tree shrew glioma exhibited aggressive behavior and a relatively short latency, and markedly reduced animal survival. Remarkably, the biological features of human high-grade glioma (necrosis, microvascular proliferation, pseudopalisading) were all present in tree shrew glioma. Furthermore, genetic analysis of tree shrew glioma revealed that the tumors were clustered within the mesenchymal subgroup of human glioblastoma multiforme. Compared with the corresponding mouse glioma, tree shrew gliomas were markedly more similar to human glioblastoma at gene expression profile. The tree shrew glioma model provides colleagues working in the field of gliomas and cancer in general with a more accurate animal model.
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China.|Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences, Key Laboratory of Bioactive Peptides of Yunnan Province, Kunming Institute of Zoology, Kunming 650223, Yunnan, China.
authors.forename: Yaohui
authors.initials: Y
authors.lastname: Tong
authors.affiliation: State Key Lab of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China.
authors.forename: Junjun
authors.initials: J
authors.lastname: Hao
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences, Key Laboratory of Bioactive Peptides of Yunnan Province, Kunming Institute of Zoology, Kunming 650223, Yunnan, China.|Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, Yunnan 650204, China.
authors.forename: Qiu
authors.initials: Q
authors.lastname: Tu
authors.affiliation: Department of Neurological Surgery, The First Affiliated Hospital, Kunming Medical University, Kunming, Yunnan, 650032, China.
authors.forename: Hualin
authors.initials: H
authors.lastname: Yu
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences, Key Laboratory of Bioactive Peptides of Yunnan Province, Kunming Institute of Zoology, Kunming 650223, Yunnan, China.|Kunming Primate Research Center, Chinese Academy of Sciences, Kunming, Yunnan 650223, China.
authors.forename: Lanzhen
authors.initials: L
authors.lastname: Yan
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences, Key Laboratory of Bioactive Peptides of Yunnan Province, Kunming Institute of Zoology, Kunming 650223, Yunnan, China.|Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, Yunnan 650204, China.|Kunming University of Science and Technology, Kunming, Yunnan, 650500, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Kunming Primate Research Center, Chinese Academy of Sciences, Kunming, Yunnan 650223, China.
authors.forename: Longbao
authors.initials: L
authors.lastname: Lv
authors.affiliation: Department of Neurological Surgery, The First Affiliated Hospital, Kunming Medical University, Kunming, Yunnan, 650032, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Institute for Cancer Genetics, Columbia University, New York, New York 10032, USA.
authors.forename: Antonio
authors.initials: A
authors.lastname: Iavarone
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences, Key Laboratory of Bioactive Peptides of Yunnan Province, Kunming Institute of Zoology, Kunming 650223, Yunnan, China.|Kunming Primate Research Center, Chinese Academy of Sciences, Kunming, Yunnan 650223, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D005909:Glioblastoma / Q000175:diagnosis* / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008297:Male; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D059467:Transcriptome / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D014418:Tupaiidae
chemical_list: D034741:RNA, Small Interfering; D016159:Tumor Suppressor Protein p53
keywords: P53; animal model; glioblastoma; tree shrew