title: Virulence genome analysis ofPseudomonas aeruginosaVRFPA10 recovered from patient with scleritis.
abstract: Infectious keratitis is a major cause of blindness, next to cataract and majority of cases are mainly caused by gram negative bacteriumPseudomonas aeruginosa (P.aeruginosa). In this study, we investigated aP.aeruginosaVRFPA10 genome which exhibited susceptibility to commonly used drugsin vitrobut the patient had poor prognosis due to its hyper virulent nature. Genomic analysis of VRFPA10 deciphered multiple virulence factors andP.aeruginosaGenomic Islands (PAGIs) VRFPA10 genome which correlated with hyper virulence nature of the organism. The genome sequence has been deposited in DDBJ/EMBL/GenBank under the accession numbers LFMZ01000001-LFMZ01000044.
authors.affiliation: Dept of Microbiology, L & T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai, Tamil Nadu 6000 06, India; Scholar, School of Chemical & Biotechnology, SASTRA University, Thanjavur, Tamil Nadu 613401, India.
authors.forename: Nandagopal
authors.initials: N
authors.lastname: Murugan
authors.affiliation: Dept of Microbiology, L & T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai, Tamil Nadu 6000 06, India.
authors.forename: Jambulingam
authors.initials: J
authors.lastname: Malathi
authors.affiliation: Centre for Bioinformatics, Vision Research Foundation, Sankara Nethralaya, Chennai, Tamil Nadu 6000 06, India.
authors.forename: Vetrivel
authors.initials: V
authors.lastname: Umashankar
authors.affiliation: Dept of Microbiology, L & T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai, Tamil Nadu 6000 06, India.
authors.forename: Hajib Narahari Rao
authors.initials: HN
authors.lastname: Madhavan
keywords: Drug resistance; Genomics; Next generation sequencing; Ocular infection; Pseudomonas aeruginosa; Scleritis; Virulence