title: Prediction of peroxide value in omega-3 rich microalgae oil by ATR-FTIR spectroscopy combined with chemometrics.
abstract: Our work explored, for the first time, monitoring peroxide value (PV) of omega-3 rich algae oil using ATR-FTIR spectroscopic technique. The PV of the developed method was compared by that obtained by standard method of Association of Official Analytical Chemists (AOAC). In this study, peak area integration (PAI), Partial Least Squares Regression (PLSR), and Principal Component Regression (PCR) were used as the calibration techniques. PV obtained by the AOAC method and by FTIR-ATR technique were well correlated considering the peak area related to trans double bonds and chemometrics techniques of PLSR and PCR. Calibration model was established using the band with a peak point at 966cm-1(990-940cm-1) related to CH out of plane deformation vibration of trans double bond. Algae oil oxidation could be successfully quantified using PAI, PLSR and PCR techniques. Additionally, hierarchical cluster analysis was performed and significant discrimination was observed coherently with oxidation process.
authors.affiliation: Yıldız Technical University, Chemical and Metallurgical Engineering Faculty, Food Engineering Department, 34210 İstanbul, Turkey.
authors.forename: Nur
authors.initials: N
authors.lastname: Cebi
authors.affiliation: Yıldız Technical University, Chemical and Metallurgical Engineering Faculty, Food Engineering Department, 34210 İstanbul, Turkey. Electronic address: mtyilmaz@yildiz.edu.tr.
authors.forename: Mustafa Tahsin
authors.initials: MT
authors.lastname: Yilmaz
authors.affiliation: Yıldız Technical University, Chemical and Metallurgical Engineering Faculty, Food Engineering Department, 34210 İstanbul, Turkey.
authors.forename: Osman
authors.initials: O
authors.lastname: Sagdic
authors.affiliation: İstanbul Technical University, Technopolis, Vitatis Biotechnology R&D Industry and Trade Limited Company, 34396 İstanbul, Turkey.
authors.forename: Hande
authors.initials: H
authors.lastname: Yuce
authors.affiliation: İstanbul Technical University, Technopolis, Vitatis Biotechnology R&D Industry and Trade Limited Company, 34396 İstanbul, Turkey.
authors.forename: Emrah
authors.initials: E
authors.lastname: Yelboga
mesh_terms: D015525:Fatty Acids, Omega-3 / Q000737:chemistry*; D058086:Microalgae / Q000737:chemistry*; D010545:Peroxides / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D015525:Fatty Acids, Omega-3; D010545:Peroxides
keywords: ATR-FTIR; Algae oil; Chemometrics; Oxidation; Peroxide value