title: Accelerated Burn Wound Closure in Mice with a New Formula Based on Traditional Medicine.
abstract: BACKGROUND
  A combination of the oils of sesame, hemp, wild pistachio, and walnut has been used for treatment of skin disorders, including wound burns, in some parts of Kerman, Iran. Evaluation of this remedy in the form of a pharmaceutical formulation in animal models can pave the way for its future application in wound burn healing in humans.
  
  
  OBJECTIVE
  This experimental study investigated the healing potential of a new formula (NF) based on folk medicine from Iran for the treatment of third degree burns in mice. The formula was a combination of the oils of four plants: sesame (Sesamum indicumL.), wild pistachio (Pistacia atlanticaDesf.), hemp (Cannabis sativaL.), and walnut (Juglans regiaL.).
  
  
  METHODS
  Twenty-four mice were selected based on simple random sampling. Twenty-five percent of the total body surface area was burned by exposure to boiling water, according to the Walker-Mason method. NF and silver sulfadiazine (the positive control) were applied topically twice a day for 21 days. The burned area in the negative control group was left untreated. Epithelialization time and the percent of wound contraction were measured during the treatment period. The process of wound repairing was evaluated using histological (H and E and trichrome staining) and immunohistological (anti-pancytokeratin) methods.
  
  
  RESULTS
  When compared to the controls, NF significantly improved wound contraction after day 10. Epithelialization time in the NF group was significantly faster than in the other groups (20 vs. 25.5 days) (P < 0.001). Histopathological and immunohistochemical findings confirmed the efficacy of the NF.
  
  
  CONCLUSIONS
  A new therapeutic remedy was introduced for the treatment of burn wounds. Further clinical and molecular studies are suggested to determine the exact mechanism(s) involved in the burn wound healing effect of NF.
authors.affiliation: Department of Pharmacology, Iran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Mehrnaz
authors.initials: M
authors.lastname: Mehrabani
authors.affiliation: Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, IR Iran.
authors.forename: Seyyed Mohsen
authors.initials: SM
authors.lastname: Seyyedkazemi
authors.affiliation: Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, IR Iran.
authors.forename: Mohammad Hadi
authors.initials: MH
authors.lastname: Nematollahi
authors.affiliation: Department of Pathology, Kerman University of Medical Sciences, Kerman, IR Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Jafari
authors.affiliation: Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, IR Iran.
authors.forename: Mitra
authors.initials: M
authors.lastname: Mehrabani
authors.affiliation: Department of Pharmacology and Toxicology, School of Pharmacy, Kerman University of Medical Sciences, Kerman, IR Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Mehdipour
authors.affiliation: Department of Pathology, Kerman University of Medical Sciences, Kerman, IR Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Sheikhshoaee
authors.affiliation: Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, IR Iran; Department of Pharmacology and Toxicology, School of Pharmacy, Kerman University of Medical Sciences, Kerman, IR Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Mandegary
keywords: Burn; Folk Medicine; Healing