title: Comparative analysis of the transcriptional responses to low and high temperatures in three rice planthopper species.
abstract: The brown planthopper (Nilaparvata lugens, BPH), white-backed planthopper (Sogatella furcifera, WBPH) and small brown planthopper (Laodelphax striatellus, SBPH) are important rice pests in Asia. These three species differ in thermal tolerance and exhibit quite different migration and overwintering strategies. To understand the underlying mechanisms, we sequenced and compared the transcriptome of the three species under different temperature treatments. We found that metabolism-, exoskeleton- and chemosensory-related genes were modulated. In high temperature (37 °C), heat shock protein (HSP) genes were the most co-regulated; other genes related with fatty acid metabolism, amino acid metabolism and transportation were also differentially expressed. In low temperature (5 °C), the differences in gene expression of the genes for fatty acid synthesis, transport proteins and cytochrome P450 might explain why SBPH can overwinter in high latitudes, while BPH and WBPH cannot. In addition, other genes related with moulting, and membrane lipid composition might also play roles in resistance to low and high temperatures. Our study illustrates the common responses and different tolerance mechanisms of three rice planthoppers in coping with temperature change, and provides a potential strategy for pest management.
authors.affiliation: State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Science, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Hai-Jian
authors.initials: HJ
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Science, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Xue
authors.affiliation: State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Science, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Ji-Chong
authors.initials: JC
authors.lastname: Zhuo
authors.affiliation: State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Science, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Ruo-Lin
authors.initials: RL
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Science, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Hai-Jun
authors.initials: HJ
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Science, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Chuan-Xi
authors.identifier: http://orcid.org/0000-0002-7784-1188
authors.initials: CX
authors.lastname: Zhang
mesh_terms: D000064:Acclimatization / Q000235:genetics; D000818:Animals; D001208:Asia; D005786:Gene Expression Regulation; D017344:Genes, Insect; D006430:Hemiptera / Q000145:classification / Q000235:genetics*; D012275:Oryza; D013696:Temperature
keywords: DGE comparison; high temperature; low temperature; planthopper; transcriptome