title: Metabolic engineering of Saccharomyces cerevisiae for 2,3-butanediol production.
abstract: Saccharomyces cerevisiae is a work horse for production of valuable biofuels and biochemicals including 2,3-butanediol (2,3-BDO), a platform chemical with wide industrial applications for synthetic rubber, biosolvents and food additives. Recently, a cutting-edge technology of metabolic engineering has enabled S. cerevisiae to produce 2,3-BDO with high yield and productivity. These include (i) amplification of the 2,3-BDO biosynthetic pathway, (ii) redirection of carbon flux from ethanol or glycerol toward 2,3-BDO, and (iii) 2,3-BDO production from sugars derived from renewable biomass. These breakthroughs enforced S. cerevisiae to become a promising microbial host for production of 2,3-BDO.
authors.affiliation: Center for Food and Bioconvergence, Seoul National University, Seoul, 08826, Republic of Korea.|Department of Bio and Fermentation Convergence Technology and BK21 Plus Program, Kookmin University, Seoul, 02707, Republic of Korea.
authors.forename: Soo-Jung
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Gwanak 599, Gwanak-ro, Seoul, 08826, Republic of Korea.
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Gwanak 599, Gwanak-ro, Seoul, 08826, Republic of Korea.
authors.forename: Ye-Gi
authors.initials: YG
authors.lastname: Lee
authors.affiliation: Department of Bio and Fermentation Convergence Technology and BK21 Plus Program, Kookmin University, Seoul, 02707, Republic of Korea.
authors.forename: Yong-Cheol
authors.initials: YC
authors.lastname: Park
authors.affiliation: Center for Food and Bioconvergence, Seoul National University, Seoul, 08826, Republic of Korea. jhseo94@snu.ac.kr.|Department of Agricultural Biotechnology, Seoul National University, Gwanak 599, Gwanak-ro, Seoul, 08826, Republic of Korea. jhseo94@snu.ac.kr.
authors.forename: Jin-Ho
authors.identifier: http://orcid.org/0000-0002-4206-6046
authors.initials: JH
authors.lastname: Seo
mesh_terms: D000095:Acetolactate Synthase / Q000235:genetics / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism; D056804:Biofuels; D018533:Biomass; D002072:Butylene Glycols / Q000378:metabolism*; D002262:Carboxy-Lyases / Q000235:genetics / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005990:Glycerol / Q000378:metabolism; D060847:Metabolic Engineering / Q000379:methods*; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D011767:Pyruvate Decarboxylase / Q000172:deficiency / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
chemical_list: D056804:Biofuels; D002072:Butylene Glycols; D005656:Fungal Proteins; D000431:Ethanol; C026978:2,3-butylene glycol; D000429:Alcohol Oxidoreductases; C019801:butanediol dehydrogenase; D000095:Acetolactate Synthase; D002262:Carboxy-Lyases; D011767:Pyruvate Decarboxylase; C023746:acetolactate decarboxylase; D005990:Glycerol
keywords: 2,3-butanediol (2,3-BDO); 2,3-butanediol dehydrogenase; Pyruvate decarboxylase-deficient (Pdc−) yeast; Saccharomyces cerevisiae; α-acetolactate decarboxylase; α-acetolactate synthase