title: Quantification and Genotyping of Aichi Virus 1 in Water Samples in the Kathmandu Valley, Nepal.
abstract: Aichi virus 1 genomes were detected by quantitative PCR in groundwater from shallow dug (10/22) and tube wells (1/15), river water (14/14), and sewage (1/1), with the maximum concentration of 4.0 × 109copies/l. Nucleotide sequencing analysis demonstrated the prevalence of genotype B in the virus positive samples.
authors.affiliation: Interdisciplinary Center for River Basin Environment, Graduate Faculty of Interdisciplinary Research, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi, 400-8511, Japan. eharamoto@yamanashi.ac.jp.
authors.forename: Eiji
authors.identifier: 0000-0002-0126-0651
authors.initials: E
authors.lastname: Haramoto
authors.affiliation: Division of Environmental Engineering, Faculty of Engineering, Hokkaido University, North 13 West 8, Kita-ku, Sapporo, Hokkaido, 060-8628, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Kitajima
mesh_terms: D005618:Fresh Water / Q000821:virology*; D016679:Genome, Viral; D005838:Genotype; D060587:Groundwater / Q000821:virology*; D052699:Kobuvirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D009390:Nepal; D014881:Water Supply
keywords: Aichi virus 1; Groundwater; Kathmandu Valley; Picornaviridae