title: Cytomegalovirus infection alters phenotypes of different γδ T-cell subsets in renal transplant recipients with long-term stable graft function.
abstract: Cytomegalovirus (CMV) infection alters the phenotypic profiles of T-cells and NK cells in healthy and immunocompromised individuals. Here, we examined the effects of CMV infection on the phenotype and functions of γδ T-cell subsets in renal transplant recipients (RTR) stable several years after transplantation (n = 80) and healthy controls (n = 72). Differentiation status, function, and expression of HLA-DR, CD57, and LIR-1 on Vδ2-and Vδ2+γδ T-cells were examined in peripheral blood cells using flow cytometry. Percentages of Vδ2-γδ T-cells were higher in RTR who are CMV-seropositive and correlated with CMV antibody levels. Proportions of Vδ2-γδ T-cells expressing HLA-DR, CD57, or LIR-1 were increased in CMV-seropositive RTR and healthy controls compared to their seronegative counterparts. Additionally, Vδ2-γδ T-cells were skewed towards a terminally differentiated phenotype and most expressed CD8 in individuals who were CMV-seropositive. Increased expression of LIR-1 on terminally differentiated Vδ2-γδ T-cells was associated with CMV seropositivity in RTR and controls. The presence of CMV DNA in 15 RTR was associated with higher frequencies of LIR-1+ Vδ2+γδ T-cells and increased percentages of terminally differentiated effector memory cells in both γδ T-cell subsets. Our study further characterises the effects of CMV and transplantation on γδ T-cell phenotypes.
authors.affiliation: Biomedical Science, Curtin University, Bentley, Australia.|Department of Microbiology, Royal Perth Hospital, Perth, Australia.
authors.forename: Silvia
authors.identifier: 0000-0001-5405-9735
authors.initials: S
authors.lastname: Lee
authors.affiliation: Biomedical Science, Curtin University, Bentley, Australia.
authors.forename: Jacquita S
authors.initials: JS
authors.lastname: Affandi
authors.affiliation: Medicine & Pharmacology, University of Western Australia, Nedlands, Australia.|Department of Nephrology, Fiona Stanley Hospital, Murdoch, Australia.
authors.forename: Ashley B
authors.initials: AB
authors.lastname: Irish
authors.affiliation: Biomedical Science, Curtin University, Bentley, Australia.|Medicine & Pharmacology, University of Western Australia, Nedlands, Australia.
authors.forename: Patricia
authors.initials: P
authors.lastname: Price
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000032:analysis; D018959:CD57 Antigens / Q000032:analysis; D003586:Cytomegalovirus Infections / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D016130:Immunophenotyping; D016030:Kidney Transplantation; D000075362:Leukocyte Immunoglobulin-like Receptor B1 / Q000032:analysis; D008297:Male; D008875:Middle Aged; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D066027:Transplant Recipients; D055815:Young Adult
chemical_list: D015703:Antigens, CD; D018959:CD57 Antigens; D006684:HLA-DR Antigens; C105299:LILRB1 protein, human; D000075362:Leukocyte Immunoglobulin-like Receptor B1
keywords: cytomegalovirus; renal transplantation; γδ T-cells