title: Bee Venom Phospholipase A2 Ameliorates House Dust Mite Extract Induced Atopic Dermatitis Like Skin Lesions in Mice.
abstract: Atopic dermatitis (AD) is a biphasic inflammatory skin disease that is provoked by epidermal barrier defects, immune dysregulation, and increased skin infections. Previously, we have demonstrated that bvPLA2 evoked immune tolerance by inducing regulatory T cells (Treg), and thus alleviated Th2 dominant allergic asthma in mice. Here, we would like to determine whether treatment with bvPLA2 exacerbates the AD-like allergic inflammations induced by house dust mite extract (DFE) in a murine model. Epidermal thickness, immune cell infiltration, serum immunoglobulin, and cytokines were measured. Ear swelling, skin lesions, and the levels of total serum IgE and Th1/Th2 cytokines were elevated in DFE/DNCB-induced AD mice. Topical application of bvPLA2 elicited significant suppression of the increased AD symptoms, including ear thickness, serum IgE concentration, inflammatory cytokines, and histological changes. Furthermore, bvPLA2 treatment inhibited mast cell infiltration into the ear. On the other hand, Treg cell depletion abolished the anti-atopic effects of bvPLA2, suggesting that the effects of bvPLA2 depend on the existence of Tregs. Taken together, the results revealed that topical exposure to bvPLA2 aggravated atopic skin inflammation, suggesting that bvPLA2 might be a candidate for the treatment of AD.
authors.affiliation: Department of Science in Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemoon-gu, Seoul 02447, Korea. jhkh242@naver.com.
authors.forename: Kyung-Hwa
authors.initials: KH
authors.lastname: Jung
authors.affiliation: Department of Science in Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemoon-gu, Seoul 02447, Korea. bguswjd@naver.com.
authors.forename: Hyunjung
authors.initials: H
authors.lastname: Baek
authors.affiliation: Department of Science in Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemoon-gu, Seoul 02447, Korea. serif99@hanmail.net.
authors.forename: Manho
authors.initials: M
authors.lastname: Kang
authors.affiliation: Department of Clinical Korean Medicine, Graduate School, Kyung Hee Univeristy, 26 Kyungheedae-ro, Dongdaemoon-gu, Seoul 02447, Korea. zegil@naver.com.
authors.forename: Namsik
authors.initials: N
authors.lastname: Kim
authors.affiliation: Department of Science in Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemoon-gu, Seoul 02447, Korea. leesy4096@hanmail.net.
authors.forename: Seung Young
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Department of Science in Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemoon-gu, Seoul 02447, Korea. hbae@khu.ac.kr.
authors.forename: Hyunsu
authors.initials: H
authors.lastname: Bae
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001514:Bee Venoms / Q000201:enzymology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D003876:Dermatitis, Atopic / Q000097:blood / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D040001:Dermatophagoides farinae / Q000276:immunology*; D004137:Dinitrochlorobenzene; D004195:Disease Models, Animal; D007073:Immunoglobulin E / Q000097:blood; D019476:Insect Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D054467:Phospholipases A2 / Q000302:isolation & purification / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D013997:Time Factors
chemical_list: D018926:Anti-Allergic Agents; D001514:Bee Venoms; D016207:Cytokines; D004137:Dinitrochlorobenzene; D019476:Insect Proteins; D007073:Immunoglobulin E; D054467:Phospholipases A2
keywords: IgE; atopic dermatitis; bvPLA2; mast cell; skin lesion