title: Technological advances for interrogating the human kinome.
abstract: There is increasing appreciation among researchers and clinicians of the value of investigating biology and pathobiology at the level of cellular kinase (kinome) activity. Kinome analysis provides valuable opportunity to gain insights into complex biology (including disease pathology), identify biomarkers of critical phenotypes (including disease prognosis and evaluation of therapeutic efficacy), and identify targets for therapeutic intervention through kinase inhibitors. The growing interest in kinome analysis has fueled efforts to develop and optimize technologies that enable characterization of phosphorylation-mediated signaling events in a cost-effective, high-throughput manner. In this review, we highlight recent advances to the central technologies currently available for kinome profiling and offer our perspectives on the key challenges remaining to be addressed.
authors.affiliation: Department of Biochemistry, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada.|Vaccine and Infectious Disease Organization - International Vaccine Center, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E3, Canada.
authors.forename: Akanksha
authors.initials: A
authors.lastname: Baharani
authors.affiliation: The Centre for Applied Genomics, Genetics and Genome Biology, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.
authors.forename: Brett
authors.initials: B
authors.lastname: Trost
authors.affiliation: Department of Computer Science, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5C9, Canada.
authors.forename: Anthony
authors.initials: A
authors.lastname: Kusalik
authors.affiliation: Department of Biochemistry, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada scott.napper@usask.ca.|Vaccine and Infectious Disease Organization - International Vaccine Center, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E3, Canada.
authors.forename: Scott
authors.initials: S
authors.lastname: Napper
mesh_terms: D000818:Animals; D006801:Humans; D010766:Phosphorylation; D040081:Protein Array Analysis / Q000379:methods*; D011494:Protein Kinases / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D015398:Signal Transduction
chemical_list: D020543:Proteome; D011494:Protein Kinases
keywords: DAPPLE; PIIKA; PamChip; kinase; kinome; peptide arrays