title: RNA-binding protein PSPC1 promotes the differentiation-dependent nuclear export of adipocyte RNAs.
abstract: A highly orchestrated gene expression program establishes the properties that define mature adipocytes, but the contribution of posttranscriptional factors to the adipocyte phenotype is poorly understood. Here we have shown that the RNA-binding protein PSPC1, a component of the paraspeckle complex, promotes adipogenesis in vitro and is important for mature adipocyte function in vivo. Cross-linking and immunoprecipitation followed by RNA sequencing revealed that PSPC1 binds to intronic and 3'-untranslated regions of a number of adipocyte RNAs, including the RNA encoding the transcriptional regulator EBF1. Purification of the paraspeckle complex from adipocytes further showed that PSPC1 associates with the RNA export factor DDX3X in a differentiation-dependent manner. Remarkably, PSPC1 relocates from the nucleus to the cytoplasm during differentiation, coinciding with enhanced export of adipogenic RNAs. Mice lacking PSPC1 in fat displayed reduced lipid storage and adipose tissue mass and were resistant to diet-induced obesity and insulin resistance due to a compensatory increase in energy expenditure. These findings highlight a role for PSPC1-dependent RNA maturation in the posttranscriptional control of adipose development and function.
authors.forename: Jiexin
authors.initials: J
authors.lastname: Wang
authors.forename: Prashant
authors.initials: P
authors.lastname: Rajbhandari
authors.forename: Andrey
authors.initials: A
authors.lastname: Damianov
authors.forename: Areum
authors.initials: A
authors.lastname: Han
authors.forename: Tamer
authors.initials: T
authors.lastname: Sallam
authors.forename: Hironori
authors.initials: H
authors.lastname: Waki
authors.forename: Claudio J
authors.initials: CJ
authors.lastname: Villanueva
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Lee
authors.forename: Ronni
authors.initials: R
authors.lastname: Nielsen
authors.forename: Susanne
authors.initials: S
authors.lastname: Mandrup
authors.forename: Karen
authors.initials: K
authors.lastname: Reue
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Young
authors.forename: Julian
authors.initials: J
authors.lastname: Whitelegge
authors.forename: Enrique
authors.initials: E
authors.lastname: Saez
authors.forename: Douglas L
authors.initials: DL
authors.lastname: Black
authors.forename: Peter
authors.initials: P
authors.lastname: Tontonoz
mesh_terms: D041721:3T3-L1 Cells; D021581:Active Transport, Cell Nucleus / Q000235:genetics; D017667:Adipocytes / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D053487:DEAD-box RNA Helicases; D004734:Energy Metabolism / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D041681:NIH 3T3 Cells; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D009765:Obesity / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D020365:RNA Helicases / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism
chemical_list: C489921:Ebf1 protein, mouse; D009687:Nuclear Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D015534:Trans-Activators; C494389:paraspeckle protein 1, mouse; D053487:DEAD-box RNA Helicases; C514697:Ddx3x protein, mouse; D020365:RNA Helicases