title: Attenuation of partial unilateral ureteral obstruction - induced renal damage with hyperbaric oxygen therapy in a rat model.
abstract: OBJECTIVE
  The objective of the present study was to evaluate the effectiveness of HBO therapy on biochemical parameters, renal morphology and renal scintigraphy in rats undergoing chronic unilateral partial ureteral obstruction (UPUO).
  
  
  METHODS
  Thirty-five rats were divided into five equal groups: Control group; Sham group; HBO group; UPUO group and UPUO/HBO group. The effects of HBO therapy were examined using biochemical parameters and histopathological changes. After calculating the score for each histopathological change, the total histopathological score was obtained by adding all the scores. In addition, dynamic renal scintigraphy findings were evaluated.
  
  
  RESULTS
  Serum parameters indicating inflammation, serum tumor necrosis factoralpha, ischemia modified-albumin, IMA/albumin ratio and Pentraxin-3 levels, were observed to be high in the UPUO group and low in the UPUO/HBO treatment group. Similarly, in the treatment group, the reduction in malondialdehyde, total oxidant status and oxidative stress index levels and increase in total antioxidant capacity values were observed to be statistically significant compared to the UPUO group (p<0.001, p=0.007, p<0.001, p=0.001, respectively). The total score and apoptosis index significantly decreased after administration of HBO treatment. Dynamic 99mTc-MAG3 renal scintigraphy also showed convincing evidence regarding the protective nature of HBO against kidney injury. In the UPUO/HBO therapy group, the percentage contribution of each operated kidney increased significantly compared to the UPUO group (41.73% versus 32.72%).
  
  
  CONCLUSIONS
  The findings of this study indicate that HBO therapy had a reno-protective effect by reducing inflammation and oxidative stress, and preserving renal function after renal tissue damage due to induction of UPUO.
authors.affiliation: Department of Urology, Canakkale Onsekiz Mart University, Faculty of Medicine, Canakkale, Turkey.
authors.forename: Eyup Burak
authors.initials: EB
authors.lastname: Sancak
authors.affiliation: Department of Nuclear Medicine, Canakkale Onsekiz Mart University, Faculty of Medicine, Canakkale, Turkey.
authors.forename: Yusuf Ziya
authors.initials: YZ
authors.lastname: Tan
authors.affiliation: Department of Biochemistry, Canakkale Onsekiz Mart University, Faculty of Medicine, Canakkale, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Turkon
authors.affiliation: Department of Pharmacology, Canakkale Onsekiz Mart University, Faculty of Medicine, Canakkale, Turkey.
authors.forename: Coskun
authors.initials: C
authors.lastname: Silan
mesh_terms: D058186:Acute Kidney Injury / Q000097:blood / Q000000981:diagnostic imaging / Q000209:etiology* / Q000517:prevention & control*; D000818:Animals; D015415:Biomarkers / Q000097:blood; D004195:Disease Models, Animal; D006931:Hyperbaric Oxygenation; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D014517:Ureteral Obstruction / Q000097:blood / Q000150:complications* / Q000628:therapy*
chemical_list: D015415:Biomarkers
keywords: Apoptosis; Hyperbaric Oxygenation ; Ureteral Obstruction