title: Toll-like receptor agonists Porphyromonas gingivalis LPS and CpG differentially regulate IL-10 competency and frequencies of mouse B10 cells.
abstract: OBJECTIVE
  This study is to determine the effects of P. gingivalis LPS and CpG on B10 cell expansion and IL-10 competency in vitro.
  
  
  METHODS
  Spleen B cells were isolated from C57BL/6J mice with or without formalin-fixed P. gingivalis immunization. B cells were cultured for 48 hours under the following conditions: CD40L, CD40L+LPS, CD40L+CpG, and CD40L+LPS+CpG in the presence or absence of fixed P. gingivalis. Percentages of CD1dhiCD5+ B cells were measured by flow cytometry. IL-10 mRNA expression and secreted IL-10 were measured by real-time quantitative PCR and by ELISA respectively.
  
  
  RESULTS
  P. gingivalis LPS plus CD40L significantly increased CD1dhiCD5+ B cell percentages and secreted IL-10 levels in both immunized and non-immunized mice B cells in the presence or absence of P. gingivalis, compared with control group. Secreted IL-10 levels were significantly increased in CD40L+LPS treated group compared with CD40L treatment group in the absence of P. gingivalis. CpG plus CD40L significantly decreased CD1dhiCD5+ B cell percentages, but greatly elevated secreted IL-10 levels in immunized and non-immunized mice B cells in the absence of P. gingivalis, compared with CD40L treatment group.
  
  
  CONCLUSIONS
  P. gingivalis LPS and CpG differentially enhance IL-10 secretion and expansion of mouse B10 cells during innate and adaptive immune responses.
authors.affiliation: The Forsyth Institute, Department of Immunology and Infectious Diseases, Cambridge, Massachusetts, United States.|Capital Medical University, Beijing ChaoYang Hospital, Department of Stomatology, Beijing, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: The Forsyth Institute, Department of Immunology and Infectious Diseases, Cambridge, Massachusetts, United States.
authors.forename: Yang
authors.initials: Y
authors.lastname: Hu
authors.affiliation: The Forsyth Institute, Department of Immunology and Infectious Diseases, Cambridge, Massachusetts, United States.|Sichuan University, West China School of Stomatology, State Key Laboratory of Oral Diseases, Chengdu, Sichuan, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Yu
authors.affiliation: Capital Medical University, Beijing ChaoYang Hospital, Department of Stomatology, Beijing, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Lin
authors.affiliation: The Forsyth Institute, Department of Immunology and Infectious Diseases, Cambridge, Massachusetts, United States.
authors.forename: Grace
authors.initials: G
authors.lastname: Huang
authors.affiliation: The Forsyth Institute, Department of Immunology and Infectious Diseases, Cambridge, Massachusetts, United States.
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Kawai
authors.affiliation: The Forsyth Institute, Department of Immunology and Infectious Diseases, Cambridge, Massachusetts, United States.
authors.forename: Martin
authors.initials: M
authors.lastname: Taubman
authors.affiliation: Capital Medical University, Beijing ChaoYang Hospital, Department of Stomatology, Beijing, China.
authors.forename: Zuomin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: The Forsyth Institute, Department of Immunology and Infectious Diseases, Cambridge, Massachusetts, United States.
authors.forename: Han
authors.initials: H
authors.lastname: Xiaozhe
mesh_terms: D000818:Animals; D060151:B-Lymphocytes, Regulatory / Q000276:immunology*; D023201:CD40 Ligand / Q000502:physiology*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D007113:Immunity, Innate; D016753:Interleukin-10 / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000502:physiology*; D008810:Mice, Inbred C57BL; D016966:Porphyromonas gingivalis / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction; D013154:Spleen / Q000166:cytology; D013997:Time Factors; D051197:Toll-Like Receptor 4 / Q000819:agonists* / Q000502:physiology; D051217:Toll-Like Receptor 9 / Q000819:agonists* / Q000502:physiology
chemical_list: D008070:Lipopolysaccharides; D012333:RNA, Messenger; D051197:Toll-Like Receptor 4; D051217:Toll-Like Receptor 9; D016753:Interleukin-10; D023201:CD40 Ligand