title: Investigating the level of salivary endothelin-1 in premalignant and malignant lesions.
abstract: OBJECTIVE
  This study aimed to investigate the level of salivary endothelin-1 in premalignant and malignant lesions.
  
  
  METHODS
  In this study, 75 cases were investigated of which 25 cases were healthy, 25 cases had oral lichen planus (OLP), and 25 cases had oral squamous cell carcinoma (OSCC). In order to collect the saliva samples, the unstimulated spitting was used. The samples were collected between 9 and 12 a.m. They were sent to the lab shortly after being collected and salivary endothelin-1 was recorded for each sample according to the instruction of factory by using enzyme-linked immunosorbent assay and optical density at a wavelength of 450 nm. SPSS version 20 and one-way ANOVA and LSD tests were used to analyze the data.
  
  
  RESULTS
  The mean of salivary endothelin-1 level in patients with OSCC was 163.98 pg/ml, in patients with OLP was 160.9 pg/ml, and in healthy people was 137.19 pg/ml. The analysis of one-way ANOVA suggested that the level of salivary endothelin-1 in both groups was the same and significantly higher than that in control group (p < 0.05).
  
  
  CONCLUSIONS
  The level of salivary endothelin-1 in patients with SCC and OLP was higher than that in healthy group. Thus, it can be used as the latest therapeutic protocol for oral premalignant and malignant lesions.
authors.affiliation: Assistant Professor, Oral and Dental Diease Research Center, Department of Oral Medicine, Faculty of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran.
authors.forename: Tahereh
authors.initials: T
authors.lastname: Nosratzehi
authors.affiliation: Assistant Professor, Oral and Dental Disease Research Center, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran.
authors.forename: Sirous
authors.initials: S
authors.lastname: Risbaf Fakour
authors.affiliation: Assistant Professor, Clinical Immunology Research Center and Department of Immunology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Alijani
authors.affiliation: Dentist, Zahedan University of Medical Sciences, Zahedan, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Salehi
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D019332:Endothelin-1 / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D009062:Mouth Neoplasms / Q000378:metabolism*; D011230:Precancerous Conditions / Q000378:metabolism*; D012463:Saliva / Q000737:chemistry*
chemical_list: D014408:Biomarkers, Tumor; D019332:Endothelin-1
keywords: SCC; oral lichen planus; salivary endothelin-1