title: Spinel-type manganese ferrite (MnFe2O4) microspheres: A novel affinity probe for selective and fast enrichment of phosphopeptides.
abstract: The spinel-type magnetic manganese ferrite (MnFe2O4) microspheres synthesized by simple solvothermal method were used as a novel adsorbent for selective enrichment and effective isolation of phosphopeptides. The uniform MnFe2O4magnetic affinity microspheres (MAMSs) had a narrow particle size distribution between 250 and 260nm, and displayed superparamagnetism with a saturation magnetization value of 67.0emu/g. Comprehensively, the possible formation mechanism of MnFe2O4microspheres with ferric and manganous sources as dual precursors was elucidated by comparison with those of Fe3O4nanoparticles and MnOOH nanosheets respectively with either ferric or manganous source as single precursor. It was suggested that the spherical or sheet nanostructures could be achieved via secondary recrystallization or Ostwald ripening. The MnFe2O4MAMSs probe exhibited excellent dispersibility in aqueous solution, and rapid magnetic separation within 15s, as well as good reusability. More importantly, MnFe2O4was highly selective for phosphopeptides because of the strong coordination interaction between metal ions (Fe3+and Mn2+) and phosphate groups of phosphopeptdies. This high specificity was demonstrated by effectively enriching phosphopeptides from digest mixture of β-casein and bovine serum albumin (BSA) with high content of non-phosphopeptides, and embodied further in phosphopeptides enrichment from non-fat milk digests and human serum. Consequently, the prepared MnFe2O4affinity materials are expected to possess great potential in phosphoproteome research.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry & Chemical Engineering and Center of Materials Analysis, Nanjing University, Nanjing 210023, China; Editorial Department of Journal, Guizhou Normal University, Guiyang 550001, China.
authors.forename: Xing-Yu
authors.initials: XY
authors.lastname: Long
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry & Chemical Engineering and Center of Materials Analysis, Nanjing University, Nanjing 210023, China.
authors.forename: Jia-Yuan
authors.initials: JY
authors.lastname: Li
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry & Chemical Engineering and Center of Materials Analysis, Nanjing University, Nanjing 210023, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Sheng
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry & Chemical Engineering and Center of Materials Analysis, Nanjing University, Nanjing 210023, China. Electronic address: hzlian@nju.edu.cn.
authors.forename: Hong-Zhen
authors.initials: HZ
authors.lastname: Lian
mesh_terms: D000818:Animals; D002364:Caseins / Q000737:chemistry / Q000378:metabolism; D002417:Cattle; D005290:Ferric Compounds / Q000737:chemistry*; D017895:Manganese Compounds / Q000737:chemistry*; D008863:Microspheres; D008958:Models, Molecular; D010748:Phosphopeptides / Q000737:chemistry* / Q000378:metabolism; D059748:Proteolysis; D014357:Trypsin / Q000378:metabolism
chemical_list: D002364:Caseins; D005290:Ferric Compounds; D017895:Manganese Compounds; D010748:Phosphopeptides; C551151:manganese ferrite; D014357:Trypsin
keywords: Affinity probe; MnFe(2)O(4) MAMSs; Phosphopeptides enrichment; Spinel