title: Multi-institutional study of nuclear KIFC1 as a biomarker of poor prognosis in African American women with triple-negative breast cancer.
abstract: Nuclear KIFC1 (nKIFC1) predicts worse outcomes in breast cancer, but its prognostic value within racially distinct triple-negative breast cancer (TNBC) patients is unknown. Thus, nKIFC1 expression was assessed by immunohistochemistry in 163 African American (AA) and 144 White TNBC tissue microarrays (TMAs) pooled from four hospitals. nKIFC1 correlated significantly with Ki67 in White TNBCs but not in AA TNBCs, suggesting that nKIFC1 is not merely a surrogate for proliferation in AA TNBCs. High nKIFC1 weighted index (WI) was associated with significantly worse overall survival (OS), progression-free survival (PFS), and distant metastasis-free survival (DMFS) (Hazard Ratios [HRs] = 3.5, 3.1, and 3.8, respectively; P = 0.01, 0.009, and 0.007, respectively) in multivariable Cox models in AA TNBCs but not White TNBCs. Furthermore, KIFC1 knockdown more severely impaired migration in AA TNBC cells than White TNBC cells. Collectively, these data suggest that nKIFC1 WI an independent biomarker of poor prognosis in AA TNBC patients, potentially due to the necessity of KIFC1 for migration in AA TNBC cells.
authors.affiliation: Georgia State University, Department of Biology, Atlanta, GA, USA.
authors.forename: Angela
authors.initials: A
authors.lastname: Ogden
authors.affiliation: Georgia State University, Department of Biology, Atlanta, GA, USA.
authors.forename: Chakravarthy
authors.initials: C
authors.lastname: Garlapati
authors.affiliation: Emory University School of Medicine, Department of Pathology, Atlanta, GA, USA.
authors.forename: Xiaoxian Bill
authors.initials: XB
authors.lastname: Li
authors.affiliation: Georgia State University, Department of Biology, Atlanta, GA, USA.
authors.forename: Ravi Chakra
authors.initials: RC
authors.lastname: Turaga
authors.affiliation: Emory University School of Medicine, Department of Pathology, Atlanta, GA, USA.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Oprea-Ilies
authors.affiliation: Georgia State University, Department of Biology, Atlanta, GA, USA.
authors.forename: Nikita
authors.initials: N
authors.lastname: Wright
authors.affiliation: Georgia State University, Department of Biology, Atlanta, GA, USA.
authors.forename: Shristi
authors.initials: S
authors.lastname: Bhattarai
authors.affiliation: Georgia State University, Department of Biology, Atlanta, GA, USA.
authors.forename: Karuna
authors.initials: K
authors.lastname: Mittal
authors.affiliation: Georgia State University, Department of Biology, Atlanta, GA, USA.|Emory University School of Medicine, Department of Pathology, Atlanta, GA, USA.
authors.forename: Ceyda Sönmez
authors.initials: CS
authors.lastname: Wetherilt
authors.affiliation: Emory University School of Medicine, Department of Pathology, Atlanta, GA, USA.
authors.forename: Uma
authors.initials: U
authors.lastname: Krishnamurti
authors.affiliation: Emory University School of Medicine, Department of Pathology, Atlanta, GA, USA.
authors.forename: Michelle D
authors.initials: MD
authors.lastname: Reid
authors.affiliation: Northside Hospital Cancer Institute, Atlanta, GA, USA.
authors.forename: Mildred
authors.initials: M
authors.lastname: Jones
authors.affiliation: West Georgia Medical Center, Department of Pathology, LaGrange, GA, USA.
authors.forename: Meenakshi
authors.initials: M
authors.lastname: Gupta
authors.affiliation: Georgia State University, Department of Mathematics and Statistics, Atlanta, GA, USA.
authors.forename: Remus
authors.initials: R
authors.lastname: Osan
authors.affiliation: Emory University School of Medicine, Department of Pathology, Atlanta, GA, USA.
authors.forename: Sonal
authors.initials: S
authors.lastname: Pattni
authors.affiliation: Georgia State University, Department of Biology, Atlanta, GA, USA.
authors.forename: Ansa
authors.initials: A
authors.lastname: Riaz
authors.affiliation: Georgia State University, Department of Biology, Atlanta, GA, USA.
authors.forename: Sergey
authors.initials: S
authors.lastname: Klimov
authors.affiliation: Scott and White Medical Center, BSWHealth, Temple, TX, USA.
authors.forename: Arundhati
authors.initials: A
authors.lastname: Rao
authors.affiliation: Northside Hospital Cancer Institute, Atlanta, GA, USA.
authors.forename: Guilherme
authors.initials: G
authors.lastname: Cantuaria
authors.affiliation: Georgia State University, Department of Biology, Atlanta, GA, USA.|Novazoi Theranostics, Rolling Hills Estates, CA, USA.
authors.forename: Padmashree C G
authors.initials: PC
authors.lastname: Rida
authors.affiliation: Georgia State University, Department of Biology, Atlanta, GA, USA.
authors.forename: Ritu
authors.initials: R
authors.lastname: Aneja
mesh_terms: D001741:Black or African American; D014408:Biomarkers, Tumor / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D002467:Cell Nucleus / Q000378:metabolism*; D049109:Cell Proliferation; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D016547:Kinesins / Q000378:metabolism*; D011379:Prognosis; D016019:Survival Analysis; D064726:Triple Negative Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D044465:White People
chemical_list: D014408:Biomarkers, Tumor; C486566:KIFC1 protein, human; D016547:Kinesins