title: 2BOB - extracting an H2BC and an HSQC-type spectrum from the same data set, and H2OBC - a fast experiment delineating the protonated13C backbone.
abstract: Two new related methods, 2BOB and H2OBC, for tracking out the backbone of protonated13C nuclei are presented. 2BOB extracts an H2BC and an HSQC-type spectrum from one and the same data set, and the combined information of these two spectra tracks out the molecular backbone. The faster method, H2OBC, typically requiring only a few minutes of instrument time, yields a single spectrum with distinct and different phases imposed on the H2BC and one-bond peaks thus obviating the need to separate them in the absence of complicating spectral overlap. Copyright © 2017 John Wiley & Sons, Ltd.
authors.affiliation: Bruker UK Limited, Banner Lane, Coventry, CV4 9GH, UK.
authors.forename: Ēriks
authors.identifier: http://orcid.org/0000-0002-2366-0269
authors.initials: Ē
authors.lastname: Kupče
authors.affiliation: Copenhagen, Denmark.
authors.forename: Ole W
authors.initials: OW
authors.lastname: Sørensen
keywords: 2BOB; COSY; H2BC; H2OBC; HMQC; HSQC; quinine; structure elucidation; tobramycin