title: Significance of Anti-HLA Antibodies on Adult and Pediatric Heart Allograft Outcomes.
abstract: As methods for human leukocyte antigens (HLA) antibody detection have evolved and newer solid phase assays are much more sensitive, the last 15 years has seen a renewed focus on the importance of HLA antibodies in solid organ transplant rejection. However, there is still much controversy regarding the clinical significance of antibody level as depicted by the mean fluorescence intensity of a patient's neat serum. Emerging techniques, including those that identify antibody level and function, show promise for the detection of individuals at risk of allograft rejection, determination of the effectiveness of desensitization prior to transplant, and for monitoring treatment of rejection. Here, we review current publications regarding the relevance of donor-specific HLA antibodies (DSA) in adult and pediatric heart transplantation (HT) with graft survival, development of antibody-mediated rejection and cardiac allograft vasculopathy (CAV). The negative impact of DSA on patient and allograft survival is evident in adult and pediatric HT recipients. Many questions remain regarding the most appropriate frequency of assessment of pre- and posttransplant DSA as well as the phenotype of DSA memory vs. truede novoantibody using large multicenter adult and pediatric cohorts and state-of-the-art methodologies for DSA detection and characterization.
authors.affiliation: Division of Transplant Pathology, Department of Pathology, University of Pittsburgh Medical Center , Pittsburgh, PA , USA.
authors.forename: Massimo
authors.initials: M
authors.lastname: Mangiola
authors.affiliation: Division of Transplant Pathology, Department of Pathology, University of Pittsburgh Medical Center , Pittsburgh, PA , USA.
authors.forename: Marilyn
authors.initials: M
authors.lastname: Marrari
authors.affiliation: Pediatric Cardiology, The Children's Hospital of Pittsburgh of UPMC , Pittsburgh, PA , USA.
authors.forename: Brian
authors.initials: B
authors.lastname: Feingold
authors.affiliation: Division of Transplant Pathology, Department of Pathology, University of Pittsburgh Medical Center , Pittsburgh, PA , USA.
authors.forename: Adriana
authors.initials: A
authors.lastname: Zeevi
keywords: AMR; allograft vasculopathy; donor-specific antibodies; graft failure; heart transplantation