title: Guibourtia tessmannii-induced fictive ejaculation in spinal male rat: involvement of D1, D2-like receptors.
abstract: BACKGROUND
  Guibourtia tessmannii (Caesalpiniaceae) is a plant traditionally used as aphrodisiac. We previously reported the pro-ejaculatory effects of the aqueous and methanol extracts of G. tesmannii in spinal male rat. However, the mechanism underlying such effects has not been elucidated.
  
  
  OBJECTIVE
  This study characterizes the dopaminergic sub-type receptors involved in G. tesmannii-induced ejaculation in male Wistar rat.
  
  
  METHODS
  Urethane-anesthetized spinal male rats were intravenously treated with saline solution (1 mL/kg, control); dopamine (0.1 μmol/kg, reference); aqueous or methanol extracts of G. tesmannii (20 mg/kg) in the absence or presence of haloperidol (0.26 μmol/kg), a nonspecific dopaminergic receptor antagonist, Sch23390 (0.26 μmol/kg), a specific D1-like receptor antagonist or, sulpiride (0.26 μmol/kg), a specific D2-like receptor antagonist. Electromyography of the bulbospongiosus muscles and intraseminal pressure were recorded after urethral, penile and drug stimulations.
  
  
  RESULTS
  Urethral and penile stimulations, intravenous injection of dopamine or, aqueous and methanol extracts of G. tesmannii always triggered the expression of rhythmic contraction of the bulbospongiosus muscles with an average mean of 3.33 ± 0.43; 7.83 ± 0.85; 9.80 ± 0.86; 0.83 ± 0.54 and 2.67 ± 0.95 contractions, respectively. The intraseminal pressure was more expressed after urethral and penile stimulations (15.66 ± 1.58 and 13.60 ± 2.40 mmHg, respectively). In rats pretreated with haloperidol, Sch23390 or sulpiride, no ejaculation was recorded after intravenous injection of G. tesmannii extracts or dopamine.
  
  
  CONCLUSIONS
  Guibourtia tesmannii-induced ejaculation requires the integrity of D1and D2-like receptors. These findings further justify the ethno-medicinal claims of G. tesmannii as an aphrodisiac.
authors.affiliation: a Animal Physiology and Phytopharmacology Laboratory , University of Dschang , Dschang , Cameroon.
authors.forename: Patrick Brice
authors.initials: PB
authors.lastname: Deeh Defo
authors.affiliation: a Animal Physiology and Phytopharmacology Laboratory , University of Dschang , Dschang , Cameroon.
authors.forename: Elvis
authors.initials: E
authors.lastname: Asongu
authors.affiliation: b Department of Animal Organisms Biology , University of Douala , Douala , Cameroon.
authors.forename: Modeste Nya
authors.initials: MN
authors.lastname: Wankeu
authors.affiliation: a Animal Physiology and Phytopharmacology Laboratory , University of Dschang , Dschang , Cameroon.
authors.forename: Esther
authors.initials: E
authors.lastname: Ngadjui
authors.affiliation: a Animal Physiology and Phytopharmacology Laboratory , University of Dschang , Dschang , Cameroon.
authors.forename: Georges Romeo
authors.initials: GR
authors.lastname: Bonsou Fazin
authors.affiliation: a Animal Physiology and Phytopharmacology Laboratory , University of Dschang , Dschang , Cameroon.
authors.forename: François Xavier
authors.initials: FX
authors.lastname: Kemka
authors.affiliation: c Laboratorio de Comportamiento Reproductivo , Escuela de Medicina Veterinaria y Zootecnia, Universidad Autonoma de Tlaxcala-Mexico , Tlaxcala , Mexico.
authors.forename: Miguel
authors.initials: M
authors.lastname: Carro-Juarez
authors.affiliation: a Animal Physiology and Phytopharmacology Laboratory , University of Dschang , Dschang , Cameroon.
authors.forename: Albert
authors.initials: A
authors.lastname: Kamanyi
authors.affiliation: d Department of Animal Biology and Physiology , University of Yaoundé , Yaoundé , Cameroon.
authors.forename: Pierre
authors.initials: P
authors.lastname: Kamtchouing
authors.affiliation: a Animal Physiology and Phytopharmacology Laboratory , University of Dschang , Dschang , Cameroon.
authors.forename: Pierre
authors.initials: P
authors.lastname: Watcho
mesh_terms: D000818:Animals; D018492:Dopamine Antagonists / Q000494:pharmacology; D004542:Ejaculation / Q000187:drug effects* / Q000502:physiology*; D007887:Fabaceae; D008297:Male; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D017447:Receptors, Dopamine D1 / Q000037:antagonists & inhibitors / Q000502:physiology*; D017448:Receptors, Dopamine D2 / Q000502:physiology*; D013124:Spinal Injuries
chemical_list: D018492:Dopamine Antagonists; D010936:Plant Extracts; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2
keywords: D1-D2 receptors; Guibourtia tessmannii; fictive ejaculation; spinal cord