title: Effect of hypohydration on postsynaptic cutaneous vasodilation and sweating in healthy men.
abstract: Hypohydration decreases cutaneous vasodilation and sweating during heat stress, but it is unknown if these decrements are from postsynaptic (i.e., sweat gland/blood vessel) alterations. The purpose of this study was to determine if hypohydration affects postsynaptic cutaneous vasodilation and sweating responses. Twelve healthy men participated in euhydrated (EU) and hypohydrated (HY) trials, with hypohydration induced via fluid restriction and passive heat stress. Changes in cutaneous vascular conductance (CVC; %max) in response to incremental intradermal infusion of the endothelium-independent vasodilator sodium nitroprusside (SNP) and the endothelium-dependent vasodilator methacholine chloride (MCh) were assessed by laser Doppler flowmetry. Local sweat rate (LSR) was simultaneously assessed at the MCh site via ventilated capsule. At the end of the last dose, maximal CVC was elicited by delivering a maximal dose of SNP (5 × 10-2M) for 30 min to both sites with simultaneous local heating (~44°C) at the SNP site. The concentration of drug needed to elicit 50% of the maximal response (log EC50) was compared between hydration conditions. The percent body mass loss was greater with HY vs. EU (-2.2 ± 0.7 vs. -0.1 ± 0.7%,P< 0.001). Log EC50of endothelium-dependent CVC was lower with EU (-3.62 ± 0.22) vs. HY (-2.93 ± 0.08;P= 0.044). Hypohydration did not significantly alter endothelium-independent CVC or LSR (bothP> 0.05). In conclusion, hypohydration attenuated endothelium-dependent CVC but did not affect endothelium-independent CVC or LSR responses. These data suggest that reductions in skin blood flow accompanying hypohydration can be partially attributed to altered postsynaptic function.
authors.affiliation: Department of Health, Human Performance, and Recreation, University of Arkansas, Fayetteville, Arkansas; and.
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Tucker
authors.affiliation: Department of Health, Human Performance, and Recreation, University of Arkansas, Fayetteville, Arkansas; and.
authors.forename: Ashley
authors.initials: A
authors.lastname: Six
authors.affiliation: Department of Health, Human Performance, and Recreation, University of Arkansas, Fayetteville, Arkansas; and.|Fitbit, San Francisco, California.
authors.forename: Nicole E
authors.initials: NE
authors.lastname: Moyen
authors.affiliation: Department of Health, Human Performance, and Recreation, University of Arkansas, Fayetteville, Arkansas; and.
authors.forename: Alf Z
authors.initials: AZ
authors.lastname: Satterfield
authors.affiliation: Department of Health, Human Performance, and Recreation, University of Arkansas, Fayetteville, Arkansas; and msganio@uark.edu.
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Ganio
mesh_terms: D000328:Adult; D001783:Blood Flow Velocity; D003681:Dehydration / Q000503:physiopathology*; D018869:Heat-Shock Response; D006801:Humans; D008297:Male; D012016:Reference Values; D012039:Regional Blood Flow; D012867:Skin / Q000098:blood supply / Q000294:innervation / Q000503:physiopathology*; D013545:Sweat Glands / Q000294:innervation / Q000503:physiopathology*; D013546:Sweating
keywords: dehydration; hyperosmolality; microdialysis; skin blood flow