title: Allyl Fluorescein Ethers as Promising Fluorescent Probes for Carbon Monoxide Imaging in Living Cells.
abstract: Recently, the fluorescent detection of carbon monoxide (CO) in living cells has attracted great attention. However, due to the lack of effective ways to construct fluorescent CO probes, fluorescent detection of CO in living cells is still in its infancy. In this paper, we report for the first time the use of allyl ether as a reaction site for construction of fluorescent CO probes. By this way, two readily available allyl fluorescein ethers were prepared, which were found to be highly selective and sensitive probes for CO in the presence of PdCl2. These probes have the merits of good stability, good water-solubility, and rapid and distinct colorimetric and remarkable fluorescent turn-on signal changes. Moreover, a very low dose of these two probes can be used to detect and track CO in living cells, indicating that these two probes could be very promising biological tools for CO detection in living systems. Overall, this work provided not only two new promising fluorescent CO probes but also a new way to devise fluorescent CO probes.
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University , 152 Luoyu Road, Wuhan, Hubei 430079, P. R. China.
authors.forename: Shumin
authors.initials: S
authors.lastname: Feng
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University , 152 Luoyu Road, Wuhan, Hubei 430079, P. R. China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Liu
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University , 152 Luoyu Road, Wuhan, Hubei 430079, P. R. China.
authors.forename: Weiyong
authors.initials: W
authors.lastname: Feng
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University , 152 Luoyu Road, Wuhan, Hubei 430079, P. R. China.
authors.forename: Guoqiang
authors.identifier: 0000-0003-2548-7284
authors.initials: G
authors.lastname: Feng
mesh_terms: D000498:Allyl Compounds / Q000737:chemistry* / Q000494:pharmacology; D002248:Carbon Monoxide / Q000032:analysis*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004987:Ethers / Q000737:chemistry* / Q000494:pharmacology; D019793:Fluorescein / Q000737:chemistry* / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D061848:Optical Imaging; D013050:Spectrometry, Fluorescence
chemical_list: D000498:Allyl Compounds; D004987:Ethers; D005456:Fluorescent Dyes; D002248:Carbon Monoxide; D019793:Fluorescein