title: Effect of Low-Dose Selenium Supplementation on the Genotoxicity, Tissue Injury and Survival of Mice Exposed to Acute Whole-Body Irradiation.
abstract: The aim of the present study is to evaluate the radioprotective effect of low-dose selenium supplementation (multiple administrations) on radiation toxicities and mortality induced by lethal dose of whole-body irradiation (WBI). For this, BALB/c mice received sodium selenite (4 μg/kg body wt) intraperitoneally for five consecutive days and subjected to WBI at an absorbed dose of 8 Gy (60Co, 1 Gy/min). Administration of sodium selenite was continued even during the post irradiation days three times a week till the end of the experiment. The radioprotective effect was evaluated in terms of the improvement in 30 days post irradiation survival, protection from DNA damage, and biochemical and histological changes in radiosensitive organs. The results indicated that low-dose sodium selenite administration did not protect the mice from radiation-induced hematopoietic and gastrointestinal injuries and subsequent mortality. However, it significantly prevented the radiation-induced genotoxicity or DNA damage in peripheral leukocytes. Further sodium selenite administration modulated the messenger RNA (mRNA) expression of GPx1, GPx2, and GPx4 in the spleen and intestine differentially and led to a significant increase in GPx activity (∼1.5 to 2-folds) in these organs. In line with this observation, sodium selenite administration reduced the level of lipid peroxidation in the intestine. In conclusion, our study shows that low-dose sodium selenite supplementation can be an effective strategy to prevent WBI-induced genotoxicity but may not have an advantage against mortality sustained during nuclear emergencies.
authors.affiliation: Radiation and Photochemistry Division, Bhabha Atomic Research Centre, Mumbai, 400085, India.|Homi Bhabha National Institute, Mumbai, 400094, India.
authors.forename: Prachi
authors.initials: P
authors.lastname: Verma
authors.affiliation: Radiation and Photochemistry Division, Bhabha Atomic Research Centre, Mumbai, 400085, India. kamit@barc.gov.in.|Homi Bhabha National Institute, Mumbai, 400094, India. kamit@barc.gov.in.
authors.forename: Amit
authors.initials: A
authors.lastname: Kunwar
authors.affiliation: Homi Bhabha National Institute, Mumbai, 400094, India.|Chemistry Division, Bhabha Atomic Research Centre, Mumbai, India.
authors.forename: K
authors.initials: K
authors.lastname: Indira Priyadarsini
mesh_terms: D000818:Animals; D004249:DNA Damage / Q000187:drug effects*; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D005767:Gastrointestinal Diseases / Q000517:prevention & control*; D007274:Injections, Intraperitoneal; D007422:Intestines / Q000187:drug effects*; D008099:Liver / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018038:Sodium Selenite / Q000008:administration & dosage* / Q000494:pharmacology*; D015996:Survival Rate; D014916:Whole-Body Irradiation
chemical_list: D018038:Sodium Selenite
keywords: Genotoxicity; Glutathione peroxidase; Radioprotection; Selenium; Survival