title: Functional analysis of five trypsin-like protease genes in the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae).
abstract: Insect midgut proteases catalyze the release of free amino acids from dietary proteins and are essential for insect normal development. To date, digestive proteases as potential candidates have made great progress in pest control. To clarify the function of trypsin-like protease genes in the digestive system of Bactrocera dorsalis, a serious pest of a wide range of tropical and subtropical fruit and vegetable crops, five trypsin genes (BdTry1, BdTry2, BdTry3, BdTry4 and BdTry5) were identified from transcriptome dataset, and the effects of feeding condition on their expression levels were examined subsequently. RNA interference (RNAi) was applied to further explore their function on the growth of B. dorsalis. The results showed that all the BdTrys in starving midgut expressed at a minimal level but up-regulated upon feeding (except BdTry3). Besides, RNAi by feeding dsRNAs to larvae proved to be an effective method to cause gene silencing and the mixed dsRNAs of the five BdTrys slowed larvae growth of B. dorsalis. The current data suggest that trypsin genes are actively involved in digestion process of B. dorsalis larvae and thereafter play crucial roles in their development.
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, PR China.
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Li
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, PR China.
authors.forename: Ming-Zhe
authors.initials: MZ
authors.lastname: Hou
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, PR China.
authors.forename: Guang-Mao
authors.initials: GM
authors.lastname: Shen
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, PR China.
authors.forename: Xue-Ping
authors.initials: XP
authors.lastname: Lu
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, PR China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, PR China.
authors.forename: Fu-Xian
authors.initials: FX
authors.lastname: Jia
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, PR China.
authors.forename: Jin-Jun
authors.initials: JJ
authors.lastname: Wang
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, PR China. Electronic address: douwei80@swu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Dou
mesh_terms: D000818:Animals; D004063:Digestion / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D019476:Insect Proteins / Q000235:genetics*; D007413:Intestinal Mucosa / Q000378:metabolism; D007814:Larva / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D012313:RNA / Q000494:pharmacology; D034622:RNA Interference; D033621:Tephritidae / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D059467:Transcriptome; D014357:Trypsin / Q000235:genetics*
chemical_list: D019476:Insect Proteins; D012313:RNA; D014357:Trypsin
keywords: RNAi; digestion; growth retardation; oriental fruit fly; trypsin-like genes