title: A new strategy to enhance the biosynthesis of trans-resveratrol by overexpressing stilbene synthase gene in elicited Vitis vinifera cell cultures.
abstract: In this work, transgenic lines of suspension cultured cells of Vitis vinifera cv. Monastrell containing the plasmid pMOG800-sts have been obtained. The cell growth of these transgenic cell lines decreased slightly as compared to non-transgenic suspension cultured cells, while cell viability was not affected. In addition, the elicitation with cyclodextrins and methyl jasmonate enhanced the production of trans-resveratrol, observing the highest levels of this compound in sts-expressing transgenic Vitis suspension cultured cells with the sts expression cassette in the forwards orientation. Moreover, the forwards 2 (F2) transgenic cell line produced the greater levels of trans-resveratrol in comparison with the non-transgenic cell line. In fact, when suspension cultured cells were treated with both elicitors, the accumulation of trans-resveratrol outside the cells in the F2 transgenic suspension cultured cells increased twice (1458 mg.L-1) as compared to non-transgenic cell lines (724 mg.L-1). In both cases, the levels of trans-resveratrol detected in the treatment with cyclodextrins and methyl jasmonate were greater than the sum of the individual treatments, and therefore we observed a synergistic effect in the presence of both elicitors. Moreover, the expression profile of sts gene in transgenic V. vinifera cell lines was similar to the expression profile detected for the endogenous sts gene in non-transgenic V. vinifera cell lines, being the expression levels greater in the treatment with methyl jasmonate and cyclodextrins, which was related to the high levels of trans-resveratrol found in the presence of both elicitors.
authors.affiliation: Grupo de Biotecnología de Frutales, Departamento de Mejora Vegetal, CEBAS-CSIC, E-30100 Murcia, Spain; College of Horticulture, Gansu Agricultural University, Lanzhou 730070, Gansu Province, PR China.
authors.forename: Mingyu
authors.initials: M
authors.lastname: Chu
authors.affiliation: Department of Plant Biology, Faculty of Biology, University of Murcia, Campus de Espinardo, E-30100 Murcia, Spain.
authors.forename: M A
authors.initials: MA
authors.lastname: Pedreño
authors.affiliation: Grupo de Biotecnología de Frutales, Departamento de Mejora Vegetal, CEBAS-CSIC, E-30100 Murcia, Spain.
authors.forename: Nuria
authors.initials: N
authors.lastname: Alburquerque
authors.affiliation: Grupo de Biotecnología de Frutales, Departamento de Mejora Vegetal, CEBAS-CSIC, E-30100 Murcia, Spain.
authors.forename: Lydia
authors.initials: L
authors.lastname: Faize
authors.affiliation: Grupo de Biotecnología de Frutales, Departamento de Mejora Vegetal, CEBAS-CSIC, E-30100 Murcia, Spain.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Burgos
authors.affiliation: Department of Plant Biology, Faculty of Biology, University of Murcia, Campus de Espinardo, E-30100 Murcia, Spain. Electronic address: lorena.almagro@um.es.
authors.forename: Lorena
authors.initials: L
authors.lastname: Almagro
mesh_terms: D000085:Acetates / Q000494:pharmacology; D000217:Acyltransferases / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D060054:Agrobacterium / Q000235:genetics; D002460:Cell Line; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D003505:Cyclodextrins / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D054883:Oxylipins / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D030821:Plants, Genetically Modified; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism*; D014170:Transformation, Genetic; D027843:Vitis / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000085:Acetates; D003505:Cyclodextrins; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D013267:Stilbenes; C072239:methyl jasmonate; D000217:Acyltransferases; C022674:stilbene synthase; D000077185:Resveratrol
keywords: Agrobacterium system; Stable transformation; Stilbene synthase; Vitis vinifera cell culture; trans-Resveratrol