title: Biochip Spray: Simplified Coupling of Surface Plasmon Resonance Biosensing and Mass Spectrometry.
abstract: A simplified coupling of surface plasmon resonance (SPR) immuno-biosensing with ambient ionization mass spectrometry (MS) was developed. It combines two orthogonal analysis techniques: the biosensing capability of SPR and the chemical identification power of high resolution MS. As a proof-of-principle, deoxynivalenol (DON), an important mycotoxin, was captured using an SPR gold chip containing an antifouling layer and monoclonal antibodies against the toxin and, after washing, the chip could be taken out and analyzed by direct spray MS of the biosensor chip to confirm the identity of DON. Furthermore, cross-reacting conjugates of DON present in a naturally contaminated beer could be successfully identified, thus showing the potential of rapid identification of (un)expected cross-reacting molecules.
authors.affiliation: Laboratory of Organic Chemistry, Wageningen University & Research , Stippeneng 4, 6708 WE Wageningen, The Netherlands.|TI-COAST , Science Park 904, 1098 XH Amsterdam, The Netherlands.
authors.forename: Sweccha
authors.initials: S
authors.lastname: Joshi
authors.affiliation: Laboratory of Organic Chemistry, Wageningen University & Research , Stippeneng 4, 6708 WE Wageningen, The Netherlands.
authors.forename: Han
authors.identifier: 0000-0001-5773-8506
authors.initials: H
authors.lastname: Zuilhof
authors.affiliation: Laboratory of Organic Chemistry, Wageningen University & Research , Stippeneng 4, 6708 WE Wageningen, The Netherlands.
authors.forename: Teris A
authors.initials: TA
authors.lastname: van Beek
authors.affiliation: Laboratory of Organic Chemistry, Wageningen University & Research , Stippeneng 4, 6708 WE Wageningen, The Netherlands.|RIKILT, Wageningen University & Research , P.O. Box 230, 6700 AE Wageningen, The Netherlands.
authors.forename: Michel W F
authors.identifier: 0000-0003-4634-0249
authors.initials: MW
authors.lastname: Nielen
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D005658:Fungi / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation; D020349:Surface Plasmon Resonance; D014255:Trichothecenes / Q000032:analysis* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D014255:Trichothecenes; D006046:Gold; C007262:deoxynivalenol