title: Multiplexed detection of various breast cancer cells by perfluorocarbon/quantum dot nanoemulsions conjugated with antibodies.
abstract: The effective targeting of cancer cell surface antigens is an attractive approach in cancer diagnosis and therapy. Multifunctional nanoprobes with cell-targeting specificity are likely to find important applications in bioanalysis, biomedicine, and clinical diagnosis. In this study, we have fabricated biocompatible perfluorocan/quantum dot nanoemulsions as bimodal imaging nanoprobes for the targeting of breast cancer cells. Perfluorocarbon/quantum dot nanoemulsions conjugated with monoclonal antibodies, as a type of bimodal imaging nanoprobe based on19F-MR and optical imaging, have been synthesized and applied for targeted imaging of three different breast cancer cells (SKBR3, MCF-7, MDA-MB 468), respectively. We have shown that the cancer-detection capabilities of antibody-conjugated PFC/QDs nanoemulsions could be successfully applied to target of various breast cancer cells. These modified PFC/QDs nanoemulsions were shown to target the cancer cell surface receptors specially. Conjugation of ligands to nanoemulsions targeting over-expressed cell surface receptors is a promising approach for targeted imaging to tumor cells. We further propose that the PFC/QDs nanoemulsions could be used in targeted imaging of breast cancer cells.
authors.affiliation: Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806 Korea.
authors.forename: Pan Kee
authors.initials: PK
authors.lastname: Bae
authors.affiliation: Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806 Korea.
authors.forename: Bong Hyun
authors.initials: BH
authors.lastname: Chung
keywords: Bimodal imaging; Breast cancer; Perfluorocarbon; Quantum dot