title: Bacterial community shift and hydrocarbon transformation during bioremediation of short-term petroleum-contaminated soil.
abstract: A laboratory study was conducted to evaluate the impact of bioaugmentation plus biostimulation (BR, added both nutrients and bacterial consortia), and natural attenuation (NA) on hydrocarbon degradation efficiency and microflora characterization during remediation of a freshly contaminated soil. After 112 days of remediation, the initial level of total petroleum hydrocarbon (TPH) (61,000 mg/kg soil) was reduced by 4.5% and 5.0% in the NA and BR treatments, respectively. Bioremediation did not significantly enhance TPH biodegradation compared to natural attenuation. The degradation of the aliphatic fraction was the most active with the degradation rate of 30.3 and 28.7 mg/kg/day by the NA and BR treatments, respectively. Soil microbial activities and counts in soil were generally greater for bioremediation than for natural attenuation. MiSeq sequencing indicated that the diversity and structure of microbial communities were affected greatly by bioremediation. In response to bioremediation treatment, Promicromonospora, Pseudomonas, Microcella, Mycobacterium, Alkanibacter, and Altererythrobacter became dominant genera in the soil. The result indicated that combining bioaugmentation with biostimulation did not improve TPH degradation, but soil microbial activities and structure of microbial communities are sensitive to bioremediation in short-term and heavily oil-contaminated soil.
authors.affiliation: Key Laboratory of Northwest Water Resources, Environment and Ecology, MOE, Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, No.13 Yanta Road, Xi'an, Shaanxi Province 710055, China. Electronic address: wumanli1974@hotmail.com.
authors.forename: Manli
authors.initials: M
authors.lastname: Wu
authors.affiliation: Key Laboratory of Northwest Water Resources, Environment and Ecology, MOE, Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, No.13 Yanta Road, Xi'an, Shaanxi Province 710055, China.
authors.forename: Xiqiong
authors.initials: X
authors.lastname: Ye
authors.affiliation: Key Laboratory of Northwest Water Resources, Environment and Ecology, MOE, Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, No.13 Yanta Road, Xi'an, Shaanxi Province 710055, China.
authors.forename: Kaili
authors.initials: K
authors.lastname: Chen
authors.affiliation: Key Laboratory of Northwest Water Resources, Environment and Ecology, MOE, Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, No.13 Yanta Road, Xi'an, Shaanxi Province 710055, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Key Laboratory of Northwest Water Resources, Environment and Ecology, MOE, Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, No.13 Yanta Road, Xi'an, Shaanxi Province 710055, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Northwest Water Resources, Environment and Ecology, MOE, Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, No.13 Yanta Road, Xi'an, Shaanxi Province 710055, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Jiang
mesh_terms: D001419:Bacteria / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002681:China; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000032:analysis; D017753:Ecosystem; D006838:Hydrocarbons / Q000378:metabolism*; D010578:Petroleum / Q000378:metabolism*; D011549:Pseudomonas / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D006838:Hydrocarbons; D010578:Petroleum; D012987:Soil; D012989:Soil Pollutants
keywords: Bioaugmentation plus biostimulation; Microbial activities; Microbial communities; Short-term contaminated soil; TPH