title: Responses of spatial-temporal dynamics of bacterioplankton community to large-scale reservoir operation: a case study in the Three Gorges Reservoir, China.
abstract: Large rivers are commonly regulated by damming, yet the effects of such disruption on bacterioplankton community structures have not been adequately studied. The aim of this study was to explore the biogeographical patterns present under dam regulation and to uncover the major drivers structuring bacterioplankton communities. Bacterioplankton assemblages in the Three Gorges Reservoir (TGR) were analyzed using Illumina Miseq sequencing by comparing seven sites located within the TGR before and after impoundment. This approach revealed ecological and spatial-temporal variations in bacterioplankton community composition along the longitudinal axis. The community was dynamic and dominated by Proteobacteria and Actinobacteria phyla, encompassing 39.26% and 37.14% of all sequences, respectively, followed by Bacteroidetes (8.67%) and Cyanobacteria (3.90%). The Shannon-Wiener index of the bacterioplankton community in the flood season (August) was generally higher than that in the impoundment season (November). Principal Component Analysis of the bacterioplankton community compositions showed separation between different seasons and sampling sites. Results of the relationship between bacterioplankton community compositions and environmental variables highlighted that ecological processes of element cycling and large dam disturbances are of prime importance in driving the assemblages of riverine bacterioplankton communities.
authors.affiliation: Key Laboratory of Reservoir Aquatic Environment of CAS, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Laboratory of Reservoir Aquatic Environment of CAS, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China.
authors.forename: Lunhui
authors.initials: L
authors.lastname: Lu
authors.affiliation: Key Laboratory of Reservoir Aquatic Environment of CAS, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China.|Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Guo
authors.affiliation: Key Laboratory of Reservoir Aquatic Environment of CAS, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China.
authors.forename: Jixiang
authors.initials: J
authors.lastname: Yang
authors.affiliation: College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China.
authors.forename: Jiachao
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China.
authors.forename: Bin
authors.initials: B
authors.lastname: He
authors.affiliation: Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Xu
mesh_terms: D041963:Bacteroidetes; D044822:Biodiversity; D002681:China; D002734:Chlorophyll / Q000032:analysis; D004777:Environment; D005843:Geography; D054892:Metagenome; D056186:Metagenomics / Q000379:methods; D010933:Plankton; D045483:Rivers / Q000382:microbiology*; D062211:Spatio-Temporal Analysis; D014871:Water Microbiology
chemical_list: D002734:Chlorophyll