title: Oral application of carbon nanofibers in rats increases blood concentration of IL6 and IL10 and decreases locomotor activity.
abstract: Carbon nanofibers (CNF) are versatile nanomaterials that are widely used in various fields of science and technology. As a consequence, animals as well as humans may be exposed to such compounds via different routes. We hypothesized that oral intake of CNF will lead to an inflammatory reaction and consequently induce behavioral impairments. To address this issue, rats were fed with 500mg/kgCNF for 14days and their locomotor activity, emotional status and cognition were quantified by testing the animals in an open field set-up, elevated plus maze and in the universal problem solving box which provides information about motivation and cognition. The behavioral tests were performed 3 times within 10days. At the end of the experiment, blood samples were collected and the plasma concentrations of IL-6, IL-8, IL-1β, IL-10 and IL-18 were measured. Our results demonstrated an inflammatory reaction determined by a significantly elevated IL-6 concentration. This, however, was counteracted by an even more pronounced increase in IL-10. The behavioral effects were restricted mainly to a decrease in locomotor activity which was significant in the open field test, as well as the elevated plus maze. Other parameters indicative of cognitive performance were not influenced and also the emotional status was largely unaffected. In conclusion, our results revealed that oral intake of 500mg/kgCNF induced some adverse effects, which, however, can be still partially compensated by the organism.
authors.affiliation: Far Eastern Federal University, Vladivostok, Russian Federation; Amur State Medical Academy, Blagoveshchensk, Russian Federation.
authors.forename: Nina Vitalievna
authors.initials: NV
authors.lastname: Sayapina
authors.affiliation: Amur State Medical Academy, Blagoveshchensk, Russian Federation.
authors.forename: Tatyana Anantolievna
authors.initials: TA
authors.lastname: Batalova
authors.affiliation: Far Eastern Federal University, Vladivostok, Russian Federation.
authors.forename: Alexander Alexandrovich
authors.initials: AA
authors.lastname: Sergievich
authors.affiliation: Amur State Medical Academy, Blagoveshchensk, Russian Federation.
authors.forename: Mikhail Anatolievich
authors.initials: MA
authors.lastname: Shtarberg
authors.affiliation: Amur State Medical Academy, Blagoveshchensk, Russian Federation.
authors.forename: Eugeny Alexandrovich
authors.initials: EA
authors.lastname: Borodin
authors.affiliation: Technical Institute (branch) of North Eastern Federal University, Nerungri, Russian Federation.
authors.forename: Pavel Pavlovich
authors.initials: PP
authors.lastname: Khoroshikh
authors.affiliation: Far Eastern Federal University, Vladivostok, Russian Federation.
authors.forename: Vladimir Victorovich
authors.initials: VV
authors.lastname: Chaika
authors.affiliation: Far Eastern Federal University, Vladivostok, Russian Federation.
authors.forename: Vladimir Valerievich
authors.initials: VV
authors.lastname: Kodintsev
authors.affiliation: Boreskov Institute of Catalysis, Novosibirsk, Russian Federation.
authors.forename: Alexey Anatolievich
authors.initials: AA
authors.lastname: Vedyagin
authors.affiliation: Boreskov Institute of Catalysis, Novosibirsk, Russian Federation.
authors.forename: Ilya Vladimirovich
authors.initials: IV
authors.lastname: Mishakov
authors.affiliation: Department of Neurosurgery, University of Crete, Medical School, Heraklion University Hospital, Voutes, 71 021 Heraklion, Crete, Greece.
authors.forename: Antonis
authors.initials: A
authors.lastname: Vakis
authors.affiliation: Institute of Medical Psychology, Otto-von-Guericke University, Magdeburg, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Henrich-Noack
authors.affiliation: Department of Forensic Sciences and Toxicology, Medical School, University of Crete, Heraklion, Greece. Electronic address: tsatsaka@uoc.gr.
authors.forename: Aristidis M
authors.initials: AM
authors.lastname: Tsatsakis
authors.affiliation: Gazi University, Faculty of Pharmacy, Department of Toxicology, Ankara, Turkey.
authors.forename: Ayse Basak
authors.initials: AB
authors.lastname: Engin
authors.affiliation: Far Eastern Federal University, Vladivostok, Russian Federation. Electronic address: droopy@mail.ru.
authors.forename: Kirill
authors.initials: K
authors.lastname: Golokhvast
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D002244:Carbon / Q000008:administration & dosage* / Q000737:chemistry; D003071:Cognition / Q000187:drug effects; D016753:Interleukin-10 / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood*; D008124:Locomotion / Q000187:drug effects*; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D057139:Nanofibers / Q000008:administration & dosage / Q000737:chemistry; D051381:Rats
chemical_list: C556062:IL10 protein, mouse; D015850:Interleukin-6; C577686:interleukin-6, mouse; D016753:Interleukin-10; D002244:Carbon
keywords: Wistar rats; behavioral indicates; carbon nanofibers; interleukin-10; interleukin-6; locomotor activity