title: Fluorescence in situ hybridization status of voided urine predicts invasive and high-grade upper tract urothelial carcinoma.
abstract: Here, we assessed the diagnostic accuracy of fluorescence in situ hybridization (FISH) for detecting aggressive upper tract urothelial carcinoma (UTUC). A total of 210 UTUC patients from a single hospital in China were enrolled in this retrospective study between 2012 and 2016. Patients were classified as FISH-positive or -negative based on FISH analysis of voided urine, and the clinicpathological characteristics of these two patient groups were compared. Patients with positive FISH results had more advanced tumor stages and higher tumor grades than those with negative results. The overall sensitivity of FISH for detecting advanced UTUC was 62.4% (131/210). The sensitivity and positive predictive values of positive FISH results were 76.5% and 59.5% for high-grade UTUC and 71.7% and 58.0% for muscle-invasive UTUC. These data suggest that voided urine FISH results accurately predict advanced UTUC and might help distinguish patients with aggressive disease from those who might benefit from conservative management.
authors.affiliation: Department of Urology, Peking University First Hospital, Xicheng, Beijing, China.|Institute of Urology, Peking University, National Urological Cancer Center, Beijing, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Su
authors.affiliation: Department of Urology, Peking University First Hospital, Xicheng, Beijing, China.|Institute of Urology, Peking University, National Urological Cancer Center, Beijing, China.
authors.forename: Han
authors.initials: H
authors.lastname: Hao
authors.affiliation: Department of Urology, Peking University First Hospital, Xicheng, Beijing, China.|Institute of Urology, Peking University, National Urological Cancer Center, Beijing, China.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Urology, Peking University First Hospital, Xicheng, Beijing, China.|Institute of Urology, Peking University, National Urological Cancer Center, Beijing, China.
authors.forename: Zhisong
authors.initials: Z
authors.lastname: He
authors.affiliation: Department of Urology, Peking University First Hospital, Xicheng, Beijing, China.|Institute of Urology, Peking University, National Urological Cancer Center, Beijing, China.
authors.forename: Kan
authors.initials: K
authors.lastname: Gong
authors.affiliation: Department of Urology, Peking University First Hospital, Xicheng, Beijing, China.|Institute of Urology, Peking University, National Urological Cancer Center, Beijing, China.
authors.forename: Cuijian
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Urology, Peking University First Hospital, Xicheng, Beijing, China.|Institute of Urology, Peking University, National Urological Cancer Center, Beijing, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Cai
authors.affiliation: Department of Urology, Peking University First Hospital, Xicheng, Beijing, China.|Institute of Urology, Peking University, National Urological Cancer Center, Beijing, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Urology, Peking University First Hospital, Xicheng, Beijing, China.|Institute of Urology, Peking University, National Urological Cancer Center, Beijing, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yao
authors.affiliation: Department of Urology, Peking University First Hospital, Xicheng, Beijing, China.|Institute of Urology, Peking University, National Urological Cancer Center, Beijing, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Department of Urology, Peking University First Hospital, Xicheng, Beijing, China.|Institute of Urology, Peking University, National Urological Cancer Center, Beijing, China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Gong
authors.affiliation: Department of Urology, Peking University First Hospital, Xicheng, Beijing, China.|Institute of Urology, Peking University, National Urological Cancer Center, Beijing, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Fang
authors.affiliation: Department of Urology, Peking University First Hospital, Xicheng, Beijing, China.|Institute of Urology, Peking University, National Urological Cancer Center, Beijing, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Urology, Peking University First Hospital, Xicheng, Beijing, China.|Institute of Urology, Peking University, National Urological Cancer Center, Beijing, China.
authors.forename: Liqun
authors.initials: L
authors.lastname: Zhou
mesh_terms: D000368:Aged; D000073888:Cell-Free Nucleic Acids / Q000652:urine; D004273:DNA, Neoplasm / Q000652:urine; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D000073890:Liquid Biopsy; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D011379:Prognosis; D047368:Tumor Burden; D014571:Urologic Neoplasms / Q000175:diagnosis* / Q000235:genetics* / Q000652:urine
chemical_list: D000073888:Cell-Free Nucleic Acids; D004273:DNA, Neoplasm
keywords: conservative management; fluorescence in situ hybridization (FISH); high grade; radical nephroureterectomy (RNU); upper tract urothelial carcinoma (UTUC)