title: CD103+ lung dendritic cells (LDCs) induce stronger Th1/Th17 immunity to a bacterial lung infection than CD11bhiLDCs.
abstract: Recent studies suggest differential roles for CD103+ and CD11bhilung dendritic cells (LDCs) in host defense against viral and bacterial infections. In this study, we examined the contribution of these LDC subsets in protective immunity to chlamydial lung infection using a Chlamydia muridarum mouse infection model. We found that CD103+ LDCs showed higher expression of costimulatory molecules (CD40, CD80 and CD86) and increased production of cytokines (IL-12p70, IL-10, IL-23 and IL-6) compared with CD11bhiLDCs, but the expression of programmed death-ligand 1 (PD-L1) was similar between the two subsets. More importantly, we found, in adoptive transfer experiments, that the mice receiving CD103+ LDCs from Chlamydia-infected mice exhibited better protection than the recipients of CD11bhiLDCs, which was associated with more robust Th1/Th17 cytokine responses. In addition, in vitro experiments showed that CD103+ LDCs induced stronger IFN-γ and IL-17 responses, when cocutured with chlamydial antigen-primed CD4+ T cells, than CD11bhiLDCs. Furthermore, the blockade of PD1 in the culture of CD4+ T cells with either CD103+ or CD11bhiLDCs enhanced production of IFN-γ and IL-17. In conclusion, our data provide direct evidence that CD103+ LDCs are more potent in promoting Th1/Th17 immunity to chlamydial lung infection than CD11bhiLDCs.
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, MB, Canada.|Department of Immunology, Faculty of Medicine, University of Manitoba, Winnipeg, MB, Canada.
authors.forename: Sudhanshu
authors.initials: S
authors.lastname: Shekhar
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, MB, Canada.|Department of Immunology, Faculty of Medicine, University of Manitoba, Winnipeg, MB, Canada.
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, MB, Canada.|Department of Immunology, Faculty of Medicine, University of Manitoba, Winnipeg, MB, Canada.
authors.forename: Shuhe
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, MB, Canada. yangxi@cc.umanitoba.ca.|Department of Immunology, Faculty of Medicine, University of Manitoba, Winnipeg, MB, Canada. yangxi@cc.umanitoba.ca.
authors.forename: Xi
authors.initials: X
authors.lastname: Yang
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D039481:CD11b Antigen / Q000378:metabolism*; D002689:Chlamydia / Q000502:physiology; D002690:Chlamydia Infections / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D006367:HeLa Cells; D006801:Humans; D007109:Immunity; D039001:Integrin alpha Chains / Q000378:metabolism*; D007371:Interferon-gamma / Q000378:metabolism; D020381:Interleukin-17 / Q000378:metabolism; D008168:Lung / Q000382:microbiology; D008213:Lymphocyte Activation / Q000276:immunology; D008810:Mice, Inbred C57BL; D010641:Phenotype; D012141:Respiratory Tract Infections / Q000276:immunology / Q000382:microbiology*; D018417:Th1 Cells / Q000276:immunology*; D058504:Th17 Cells / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D039481:CD11b Antigen; D039001:Integrin alpha Chains; D020381:Interleukin-17; C091367:alpha E integrins; D007371:Interferon-gamma
keywords: Chlamydia muridarum; Th1/Th17 cells; lung dendritic cell