title: Increased Serum CD14 Level Is Associated with Depletion of TNF-α in Monocytes in Migraine Patients during Interictal Period.
abstract: The aim of the present study was to investigate the levels of circulating CD14 in relation to the expression of tumor necrosis factor alpha (TNF-α) in monocytes, and serum levels of TNF-α and macrophage inflammatory protein-1 (MIP-1) in migraine patients. Numerous studies revealed controversial changes in the components of the immune system during attacks and the interictal period in migraine patients. Our study included 40 migraineurs and 39 controls. The levels of TNF-α, MIP-1 and CD14 were measured in peripheral monocytes and in sera with the Enzyme-Linked Immunosorbent Assay (ELISA) method, and the monocyte expression of TNF-α was also analysed by immunostaining. Serum CD14 concentrations were higher and the expression of TNF-α in monocytes was decreased in migraineurs. The serum MIP-1 level correlated with Verbal Rating Scale (VRS); the MIP-1:CD14 ratio in monocytes correlated with Visual Analogue Scale (VAS); the MIP-1:CD14 ratio correlated with Migraine Severity (MIGSEV)-Pain scores; and serum CD14 concentration correlated with migraine duration in years. Increased serum CD14 and depletion of TNF-α in monocytes can orchestrate other components of the immune system during the interictal period.
authors.affiliation: Department of Neurochemistry and Neuropathology, Chair of Neurology, Poznan University of Medical Sciences (PUMS), Poznan 60-355, Poland. slamic@yahoo.com.
authors.forename: Slawomir
authors.initials: S
authors.lastname: Michalak
authors.affiliation: Department of Neurochemistry and Neuropathology, Chair of Neurology, Poznan University of Medical Sciences (PUMS), Poznan 60-355, Poland.
authors.forename: Alicja
authors.initials: A
authors.lastname: Kalinowska-Lyszczarz
authors.affiliation: Chair of Neurology, PUMS, Poznan 60-355, Poland. neurosk@ump.edu.pl.
authors.forename: Danuta
authors.initials: D
authors.lastname: Wegrzyn
authors.affiliation: Chair of Neurology, PUMS, Poznan 60-355, Poland. adamniezgoda@wp.pl.
authors.forename: Adam
authors.initials: A
authors.lastname: Niezgoda
authors.affiliation: Department of Clinical Neuroimmunology, Chair of Neurology, PUMS, Poznan 60-355, Poland. jlosy@ump.edu.pl.
authors.forename: Jacek
authors.initials: J
authors.lastname: Losy
authors.affiliation: Department of Neurochemistry and Neuropathology, Chair of Neurology, Poznan University of Medical Sciences (PUMS), Poznan 60-355, Poland. osztynowiczkr@ump.edu.pl.
authors.forename: Krystyna
authors.initials: K
authors.lastname: Osztynowicz
authors.affiliation: Chair of Neurology, PUMS, Poznan 60-355, Poland. wkozubski@ump.edu.pl.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Kozubski
mesh_terms: D000328:Adult; D015415:Biomarkers; D005260:Female; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000097:blood*; D008297:Male; D008875:Middle Aged; D008881:Migraine Disorders / Q000175:diagnosis / Q000209:etiology / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*; D012720:Severity of Illness Index; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D018950:Lipopolysaccharide Receptors; D014409:Tumor Necrosis Factor-alpha
keywords: CD-14; macrophage inflammatory protein-1 (MIP-1); migraine; monocytes; tumor necrosis factor alpha (TNF-α)