title: Expanding the product portfolio of fungal type I fatty acid synthases.
abstract: Fungal type I fatty acid synthases (FASs) are mega-enzymes with two separated, identical compartments, in which the acyl carrier protein (ACP) domains shuttle substrates to catalytically active sites embedded in the chamber wall. We devised synthetic FASs by integrating heterologous enzymes into the reaction chambers and demonstrated their capability to convert acyl-ACP or acyl-CoA from canonical fatty acid biosynthesis to short/medium-chain fatty acids and methyl ketones.
authors.affiliation: Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.|Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, Dalian, China.|Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, Gothenburg, Sweden.
authors.forename: Zhiwei
authors.identifier: 0000-0002-2925-5758
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.|Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, Gothenburg, Sweden.
authors.forename: Yongjin J
authors.identifier: 0000-0002-2369-3079
authors.initials: YJ
authors.lastname: Zhou
authors.affiliation: Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.|Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, Gothenburg, Sweden.|Biopetrolia AB, Systems and Synthetic Biology, Chalmers University of Technology, Gothenburg, Sweden.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Krivoruchko
authors.affiliation: Institute of Organic Chemistry and Chemical Biology, Buchmann Institute for Molecular Life Sciences, Cluster of Excellence for Macromolecular Complexes, Goethe University Frankfurt, Frankfurt am Main, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Grininger
authors.affiliation: Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, Dalian, China.|Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics, CAS, Dalian, China.
authors.forename: Zongbao K
authors.identifier: 0000-0003-0654-1193
authors.initials: ZK
authors.lastname: Zhao
authors.affiliation: Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.|Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, Gothenburg, Sweden.|Biopetrolia AB, Systems and Synthetic Biology, Chalmers University of Technology, Gothenburg, Sweden.|Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark.|Science for Life Laboratory, Royal Institute of Technology, Stockholm, Sweden.
authors.forename: Jens
authors.identifier: 0000-0002-9955-6003
authors.initials: J
authors.lastname: Nielsen
mesh_terms: D001487:Basidiomycota / Q000201:enzymology*; D055162:Biocatalysis; D054890:Fatty Acid Synthase, Type I / Q000737:chemistry / Q000378:metabolism*; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D007659:Ketones / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D015394:Molecular Structure; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
chemical_list: D005227:Fatty Acids; D007659:Ketones; D054890:Fatty Acid Synthase, Type I