title: Redox Proteomics and Platelet Activation: Understanding the Redox Proteome to Improve Platelet Quality for Transfusion.
abstract: Blood banks use pathogen inactivation (PI) technologies to increase the safety of platelet concentrates (PCs). The characteristics of PI-treated PCs slightly differ from those of untreated PCs, but the underlying reasons are not well understood. One possible cause is the generation of oxidative stress during the PI process. This is of great interest since reactive oxygen species (ROS) act as second messengers in platelet functions. Furthermore, there are links between protein oxidation and phosphorylation, another mechanism that is critical for cell regulation. Current research efforts focus on understanding the underlying mechanisms and identifying new target proteins. Proteomics technologies represent powerful tools for investigating signaling pathways involving ROS and post-translational modifications such as phosphorylation, while quantitative techniques enable the comparison of the platelet resting state versus the stimulated state. In particular, redox cysteine is a key player in platelet activation upon stimulation by different agonists. This review highlights the experiments that have provided insights into the roles of ROS in platelet function and the implications for platelet transfusion, and potentially in diseases such as inflammation and platelet hyperactivity. The review also describes the implication of redox mechanism in platelet storage considerations.
authors.affiliation: Transfusion Interrégionale Croix Rouge Suisse SA, Laboratoire de Recherche sur les Produits Sanguins, Route de la Corniche 2, 1066 Epalinges, Switzerland. giona.sonego@itransfusion.ch.
authors.forename: Giona
authors.initials: G
authors.lastname: Sonego
authors.affiliation: Transfusion Interrégionale Croix Rouge Suisse SA, Laboratoire de Recherche sur les Produits Sanguins, Route de la Corniche 2, 1066 Epalinges, Switzerland. melanie.abonnenc@itransfusion.ch.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Abonnenc
authors.affiliation: Transfusion Interrégionale Croix Rouge Suisse SA, Laboratoire de Recherche sur les Produits Sanguins, Route de la Corniche 2, 1066 Epalinges, Switzerland. jean-daniel.tissot@itransfusion.ch.|Faculté de Biologie et de Médecine, Université de Lausanne, CH-1011 Lausanne, Switzerland. jean-daniel.tissot@itransfusion.ch.
authors.forename: Jean-Daniel
authors.initials: JD
authors.lastname: Tissot
authors.affiliation: Transfusion Interrégionale Croix Rouge Suisse SA, Laboratoire de Recherche sur les Produits Sanguins, Route de la Corniche 2, 1066 Epalinges, Switzerland. michel.prudent@itransfusion.ch.
authors.forename: Michel
authors.initials: M
authors.lastname: Prudent
authors.affiliation: Transfusion Interrégionale Croix Rouge Suisse SA, Laboratoire de Recherche sur les Produits Sanguins, Route de la Corniche 2, 1066 Epalinges, Switzerland. niels.lion@itransfusion.ch.|Faculté de Biologie et de Médecine, Université de Lausanne, CH-1011 Lausanne, Switzerland. niels.lion@itransfusion.ch.
authors.forename: Niels
authors.initials: N
authors.lastname: Lion
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism*; D001793:Blood Preservation; D003545:Cysteine / Q000378:metabolism; D006801:Humans; D019255:NADPH Oxidases / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010766:Phosphorylation; D015539:Platelet Activation; D010974:Platelet Aggregation; D017713:Platelet Transfusion; D020543:Proteome; D040901:Proteomics / Q000379:methods; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D000975:Antioxidants; D020543:Proteome; D017382:Reactive Oxygen Species; D019255:NADPH Oxidases; D003545:Cysteine
keywords: activation; aggregation; blood products; phosphorylation; platelet function; platelets; reactive oxygen species; redox cysteine; transfusion