title: High prevalence of human papillomavirus (HPV) in oral mucosal lesions of patients at the Ambulatory of Oral Diagnosis of the Federal University of Sergipe, Northeastern Brazil.
abstract: OBJECTIVE
  The aim of this study was to evaluate the frequency of HPV infection and its genotypes in patients with oral lesions at the Ambulatory of Oral Diagnosis of the Federal University of Sergipe, Brazil.
  
  
  METHODS
  We conducted a molecular study with 21 patients (15 females) aged from two to 83 years with clinically detectable oral lesions. Samples were collected through exfoliation of lesions and HPV-DNA was identified using MY09/11 and GP5+/6+ primers. Genotyping was performed by multiplex PCR.
  
  
  RESULTS
  Benign, premalignant and malignant lesions were diagnosed by histopathology. HPV was detected in 17 samples. Of these, HPV-6 was detected in 10 samples, HPV-18 in four and HPV-16 in one sample. When samples were categorized by lesion types, HPV was detected in two papilloma cases (2/3), five carcinomas (5/6), one hyperplasia (1/1) and nine dysplasia cases (9/11).
  
  
  CONCLUSIONS
  Unlike other studies in the literature, we reported high occurrence of HPV in oral lesions. Further studies are required to enhance the comprehension of natural history of oral lesions.
authors.affiliation: Universidade Federal de Sergipe, Laboratório de Entomologia e Parasitologia Tropical, Departamento de Morfologia, São Cristóvão, SE, Brasil.
authors.forename: Mariana Goveia Melo
authors.initials: MG
authors.lastname: Ribeiro
authors.affiliation: Universidade Estadual Paulista, Faculdade de Medicina de Botucatu, Departamento de Patologia, Botucatu, SP, Brasil.
authors.forename: Larissa Doddi
authors.initials: LD
authors.lastname: Marcolino
authors.affiliation: Universidade Estadual Paulista, Faculdade de Medicina de Botucatu, Departamento de Patologia, Botucatu, SP, Brasil.
authors.forename: Bruna Ribeiro de Andrade
authors.initials: BR
authors.lastname: Ramos
authors.affiliation: Universidade Federal de Sergipe, Departamento de Odontologia, Aracaju, SE, Brasil.
authors.forename: Elaine Alves
authors.initials: EA
authors.lastname: Miranda
authors.affiliation: Universidade Federal de Sergipe, Departamento de Odontologia, Aracaju, SE, Brasil.
authors.forename: Cleverson Luciano
authors.initials: CL
authors.lastname: Trento
authors.affiliation: Universidade Federal de Sergipe, Laboratório de Entomologia e Parasitologia Tropical, Departamento de Morfologia, São Cristóvão, SE, Brasil.
authors.forename: Sona
authors.initials: S
authors.lastname: Jain
authors.affiliation: Universidade Federal de Sergipe, Departamento de Medicina, Aracaju, SE, Brasil.
authors.forename: Ricardo Queiroz
authors.initials: RQ
authors.lastname: Gurgel
authors.affiliation: Universidade Estadual Paulista, Faculdade de Medicina de Botucatu, Departamento de Patologia, Botucatu, SP, Brasil.
authors.forename: Márcia Guimarães da
authors.initials: MG
authors.lastname: Silva
authors.affiliation: Universidade Federal de Sergipe, Laboratório de Entomologia e Parasitologia Tropical, Departamento de Morfologia, São Cristóvão, SE, Brasil.
authors.forename: Silvio Santana
authors.initials: SS
authors.lastname: Dolabella
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D001706:Biopsy; D001938:Brazil / Q000453:epidemiology; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000821:virology; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009059:Mouth Diseases / Q000453:epidemiology* / Q000473:pathology / Q000821:virology*; D009061:Mouth Mucosa / Q000473:pathology / Q000821:virology*; D060885:Multiplex Polymerase Chain Reaction; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000235:genetics; D015995:Prevalence; D018570:Risk Assessment; D013997:Time Factors; D055815:Young Adult
chemical_list: D004279:DNA, Viral