title: A spectrophotometric screening method for avermectin oxidizing microorganisms.
abstract: A spectrophotometric screening method for avermectin oxidizing microbes by determination of 4″-oxo-avermectin was established based on the reaction between 4″-oxo-avermectin and 2,4-dinitrophenylhydrazine. Combined with a gradient HPLC assay, microorganisms capable of regioselectively oxidizing avermectin to 4″-oxo-avermectin were successfully obtained by this method.
authors.affiliation: Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, Zhejiang Province 310014, PR China; Engineering Research Center of Bioconversion and Biopurification, Ministry of Education, Zhejiang University of Technology, Hangzhou, Zhejiang Province 310014, PR China; Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, Zhejiang Province 310014, PR China. Electronic address: yuanshan@zjut.edu.cn.
authors.forename: Yuan-Shan
authors.initials: YS
authors.lastname: Wang
authors.affiliation: Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, Zhejiang Province 310014, PR China; Engineering Research Center of Bioconversion and Biopurification, Ministry of Education, Zhejiang University of Technology, Hangzhou, Zhejiang Province 310014, PR China; Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, Zhejiang Province 310014, PR China. Electronic address: hudei92@sina.com.
authors.forename: Qi-Wei
authors.initials: QW
authors.lastname: Hu
authors.affiliation: Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, Zhejiang Province 310014, PR China; Engineering Research Center of Bioconversion and Biopurification, Ministry of Education, Zhejiang University of Technology, Hangzhou, Zhejiang Province 310014, PR China; Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, Zhejiang Province 310014, PR China. Electronic address: zhengxingchang@qq.com.
authors.forename: Xing-Chang
authors.initials: XC
authors.lastname: Zheng
authors.affiliation: College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou, Zhejiang Province 310015, PR China. Electronic address: grace_zjf@126.com.
authors.forename: Jian-Fen
authors.initials: JF
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, Zhejiang Province 310014, PR China; Engineering Research Center of Bioconversion and Biopurification, Ministry of Education, Zhejiang University of Technology, Hangzhou, Zhejiang Province 310014, PR China; Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, Zhejiang Province 310014, PR China. Electronic address: zhengyg@zjut.edu.cn.
authors.forename: Yu-Guo
authors.initials: YG
authors.lastname: Zheng
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004269:DNA, Bacterial; D004798:Enzymes; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D016877:Oxidants; D010084:Oxidation-Reduction; D010659:Phenylhydrazines / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013053:Spectrophotometry / Q000379:methods*; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism
chemical_list: C517095:4''-oxo-avermectin; D004269:DNA, Bacterial; D004798:Enzymes; D016877:Oxidants; D010659:Phenylhydrazines; D012336:RNA, Ribosomal, 16S; C004787:2,4-dinitrophenylhydrazine; D007559:Ivermectin; C019264:avermectin
keywords: 2,4-Dinitrophenylhydrazine; 4″-Oxo-avermectin; Avermectin; Regioseletive oxidation