title: Enantioseparation of methamphetamine by supercritical fluid chromatography with cellulose-based packed column.
abstract: The enantiomers of methamphetamine were differentiated by supercritical fluid chromatography (SFC) with an enantioselective cellulose-based packed column. The optimization of the chromatographic conditions was achieved by changing column temperature, co-solvent proportion, additive concentration, flow rate and back pressure. In particular, the additive concentration crucially changed the resolution between the enantiomers. After determining the optimized conditions, the enantiomers of methamphetamine were successfully separated. The analytical precision, accuracy and limit of detection were checked by using the authentic standard and seized real samples. We believe that chiral SFC is a promising method for enantioseparation of forensic samples.
authors.affiliation: National Research Institute of Police Science, 6-3-1, Kashiwanoha, Kashiwa, Chiba 277-0882, Japan. Electronic address: segawa@nrips.go.jp.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Segawa
authors.affiliation: National Research Institute of Police Science, 6-3-1, Kashiwanoha, Kashiwa, Chiba 277-0882, Japan.
authors.forename: Yuko T
authors.initials: YT
authors.lastname: Iwata
authors.affiliation: National Research Institute of Police Science, 6-3-1, Kashiwanoha, Kashiwa, Chiba 277-0882, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yamamuro
authors.affiliation: National Research Institute of Police Science, 6-3-1, Kashiwanoha, Kashiwa, Chiba 277-0882, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kuwayama
authors.affiliation: National Research Institute of Police Science, 6-3-1, Kashiwanoha, Kashiwa, Chiba 277-0882, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Tsujikawa
authors.affiliation: National Research Institute of Police Science, 6-3-1, Kashiwanoha, Kashiwa, Chiba 277-0882, Japan.
authors.forename: Tatsuyuki
authors.initials: T
authors.lastname: Kanamori
authors.affiliation: National Research Institute of Police Science, 6-3-1, Kashiwanoha, Kashiwa, Chiba 277-0882, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Inoue
keywords: Enantiomer; Methamphetamine; Supercritical fluid chromatography