title: Segmental Distribution of Myosin Heavy Chain Isoforms Within Single Muscle Fibers.
abstract: Despite many studies looking at the distribution of myosin heavy chain (MHC) isoforms across a transverse section of muscle, knowledge of MHC distribution along the longitudinal axis of a single skeletal muscle fiber has been relatively overlooked. Immunocytochemistry was performed on serial sections of rat extensor digitorum longus (EDL) muscle to identify MHC types I, IIA, IIX, IIY, and IIB. Sixteen fascicles which contained a total of 362 fibers were randomly and systematically sampled from the three EDL muscles. All MHC type I and type II isoforms were expressed. Segmental expression occurred within a very limited segment. MHC isoform expression followed the accepted traditional order from I⇔IIA⇔IIX⇔IIB, however, in some samples expression of an isoform was circumvented from IIB to I or from I to IIB directly. Segmental distribution of MHC isoforms along a single muscle fiber may be because of the myonuclear domain. Anat Rec, 300:1636-1642, 2017. © 2017 Wiley Periodicals, Inc.
authors.affiliation: Anatomy Department, University of Otago, Dunedin, 9054, New Zealand.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Anatomy Department, University of Otago, Dunedin, 9054, New Zealand.
authors.forename: Maree
authors.initials: M
authors.lastname: Gould
mesh_terms: D000818:Animals; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D017208:Rats, Wistar
chemical_list: D020033:Protein Isoforms; D018995:Myosin Heavy Chains
keywords: EDL; MHC; myonuclear domain; segmental expression