title: Susceptibility and PK/PD relationships ofStaphylococcus aureusstrains from ovine and caprine with clinical mastitis against five veterinary fluoroquinolones.
abstract: Minimum inhibitory concentration (MIC) and mutant prevention concentration (MPC) of veterinary fluoroquinolones as enrofloxacin, its metabolite ciprofloxacin, danofloxacin, difloxacin and marbofloxacin againstStaphylococcus aureusstrains (n=24) isolated from milk of sheep and goats affected by clinical mastitis were evaluated. The authors have used the MIC and MPC, as well as the pharmacokinetic-pharmacodynamic relationships in plasma and milk. MIC values were significantly different between drugs, unlike MPC values. Lower MIC values were obtained for danofloxacin and difloxacin, middle and higher values for enrofloxacin, ciprofloxacin and marbofloxacin. However, differences in MPC values were not found between drugs. At conventional doses, the AUC24/MIC and AUC24/MPC ratios were close to 30-80 hours and 5-30 hours, with exception of danofloxacin, in plasma and milk. The time inside the mutant selection window (TMSW) was close to 3-6 hours for enrofloxacin, ciprofloxacin and marbofloxacin, near to 8 hours for danofloxacin and 12-22 hours for difloxacin. From these data, the mutant selection window could be higher for danofloxacin and difloxacin compared with the other fluoroquinolones tested. The authors concluded that enrofloxacin and marbofloxacin, at conventional doses, could prevent the selection of bacterial subpopulations ofS aureus, unlike danofloxacin and difloxacin, where higher doses could be used.
authors.affiliation: Department of Pharmacology, Toxicology and Legal and Forensic Medicine, Faculty of Veterinary Medicine, University of Cordoba, Campus de Rabanales, Córdoba 14.071, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Serrano-Rodríguez
authors.affiliation: Department of Pharmacology. Faculty of Veterinary Medicine, University of Murcia, Campus de Espinardo, Murcia 30.071, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Cárceles-García
authors.affiliation: Department of Pharmacology. Faculty of Veterinary Medicine, University of Murcia, Campus de Espinardo, Murcia 30.071, Spain.
authors.forename: C M
authors.initials: CM
authors.lastname: Cárceles-Rodríguez
authors.affiliation: Department of Pharmacology. Faculty of Veterinary Medicine, University of Murcia, Campus de Espinardo, Murcia 30.071, Spain.
authors.forename: M L
authors.initials: ML
authors.lastname: Gabarda
authors.affiliation: Department of Pharmacology, Toxicology and Legal and Forensic Medicine, Faculty of Veterinary Medicine, University of Cordoba, Campus de Rabanales, Córdoba 14.071, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Serrano-Caballero
authors.affiliation: Department of Pharmacology. Faculty of Veterinary Medicine, University of Murcia, Campus de Espinardo, Murcia 30.071, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Fernández-Varón
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002939:Ciprofloxacin / Q000031:analogs & derivatives / Q000494:pharmacology; D000077422:Enrofloxacin; D005260:Female; D024841:Fluoroquinolones / Q000494:pharmacology*; D015511:Goat Diseases / Q000188:drug therapy*; D006041:Goats; D008413:Mastitis / Q000188:drug therapy / Q000662:veterinary*; D008826:Microbial Sensitivity Tests / Q000706:statistics & numerical data / Q000662:veterinary; D008892:Milk / Q000382:microbiology; D009154:Mutation; D012756:Sheep; D012757:Sheep Diseases / Q000188:drug therapy*; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D016896:Treatment Outcome
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; C068581:danofloxacin; D000077422:Enrofloxacin; D002939:Ciprofloxacin; C047225:difloxacin; C080260:marbofloxacin
keywords: Fluoroquinolones; Lactating goats; Lactating sheep; Mastitis; Mutant prevention concentration; Staphylococcus aureus