title: [Effect of the traditional Chinese medicine compound Yisui Lixue decoction on apoptosis of marrow cells in rats with myelodysplastic syndrome induced by  dimethyl benzanthracene].
abstract: To investigate the effect of the traditional Chinese medicine compound Yisui Lixue decoction on apoptosis of marrow cells in rats with myelodysplastic syndrome (MDS) induced by dimethyl benzanthracene (DMBA).   Methods: The rats with MDS induced by the chemical mutagens DMBA were divided into 5 groups (12 rats in each group): a control group, a model+PBS group, a model+compound Zaofan pill group, a model+low dose of Yisui Lixue decoction group and a model+high dose of Yisui Lixue decoction group. After DMBA treatment for 14 days, rats were treated with different drugs for 1 month and executed on the 31 day. The peripheral blood cells, expressions of B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X protein (Bax), apoptosis of bone marrow cells were measured.  Results: The counts of peripheral leukocyte, erythrocyte, hemoglobin and platelet in the model+PBS group were significantly decreased compared with those in the control group (P<0.01), while the peripheral hemograms of the treatment groups were improved significantly compared with those in the model+PBS group (P<0.05 or P<0.01), especially in the model+high dose group (P<0.01). In the model+PBS group, the expressions of Bax and Bcl-2 was significantly increased (P<0.01) or decreased (P<0.01), respectively, which were reversed by treatment of Yisui Lixue decoction (P<0.05 or P<0.01), especially in the model+high dose group (P<0.01). Compared with control group, the total apoptosis rate, earlier apoptosis and later apoptosis rate of bone marrow cells in the model+PBS group were increased (P<0.01), which were blocked by treatment of Yisui Lixue decoction (P<0.05 or P<0.01), and the model+high dose group was better than the model+low dose group.   Conclusion: The traditional Chinese medicine compound Yisui Lixue decoction can improve peripheral hemogram, decrease the expression of Bax, increase the expression of Bcl-2, and reduce the apoptosis of bone marrow cells.
authors.affiliation: Department of Chinese Medicine, Chongqing Three Corges Medical College, Chongqing 404120, China.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Chongqing General Blood Institute, Chongqing 400041, China.
authors.forename: Deshang
authors.initials: D
authors.lastname: Xiong
authors.affiliation: Department of Hematology, Affiliated Children's Hospital of Chongqing Medical University, Chongqing 400014, China.
authors.forename: Youhua
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Hematology, Affiliated Children's Hospital of Chongqing Medical University, Chongqing 400014, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Hematology, Affiliated Children's Hospital of Chongqing Medical University, Chongqing 400014, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xian
authors.affiliation: Chongqing General Blood Institute, Chongqing 400041, China.
authors.forename: E
authors.initials: E
authors.lastname: Cai
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001551:Benz(a)Anthracenes; D001772:Blood Cell Count; D001853:Bone Marrow; D001854:Bone Marrow Cells / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use*; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D008516:Medicine, Chinese Traditional; D009190:Myelodysplastic Syndromes / Q000139:chemically induced / Q000188:drug therapy*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D051028:bcl-2-Associated X Protein / Q000187:drug effects / Q000378:metabolism
chemical_list: D001551:Benz(a)Anthracenes; D004365:Drugs, Chinese Herbal; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C030935:benz(a)anthracene