title: Monocytic angiotensin-converting enzyme 2 relates to atherosclerosis in patients with chronic kidney disease.
abstract: Increased levels of monocytic angiotensin-converting enzyme (ACE) found in haemodialysis (HD) patients may directly participate in the pathogenesis of atherosclerosis. We demonstrated recently that uremia triggers the development of highly pro-atherogenic monocytes via an angiotensin II (AngII)–dependent mechanism. Opposing actions of the AngII-degrading ACE2 remain largely unknown. We examined the status of both ACEs and related receptors in circulating leukocytes of HD, not-dialyzed CKD and healthy individuals. Furthermore, we tested the possible impact of monocytic ACEs on atherogenesis and behaviour of the cells under conditions mimicking chronic renal failure.
  
  
  
  Expression of ACE, ACE2, AT1R, AT2R and MASR was investigated on circulating leukocytes from 71 HD (62 ± 14 years), 24 CKD stage 3–5 (74 ± 10 years) patients and 37 healthy control subjects (53 ± 6 years) and isolated healthy monocytes treated with normal and uremic serum. Analyses of ACE, ACE2, ICAM-1, VCAM-1, MCSF and endothelial adhesion were tested on ACE-overexpressing THP-1 monocytes treated with captopril or losartan. ACE2-overexpressing monocytes were subjected to transmigration and adhesion assays and investigated for MCP-1, ICAM-1, VCAM-1, MCSF, AT1R and AT2R expression.
  
  
  
  The ACE mRNA level was significantly increased in HD and CKD stage 3–5 leukocytes. Correspondingly, ACE2 was downregulated and AngII as well as MAS receptor expression was upregulated in these cells. Healthy monocytes preconditioned with uremic serum reflected the same expressional regulation of ACE/ACE2, MAS and AngII receptors as those observed in HD and CKD stage 3–5 leukocytes. Overexpression of monocytic ACE dramatically decreased levels of ACE2 and induced a pro-atherogenic phenotype, partly reversed by AngII-modifying treatments, leading to an increase in ACE2. Overexpression of ACE2 in monocytes led to reduced endothelial adhesion, transmigration and downregulation of adhesion-related molecules.
  
  
  
  HD and not-dialyzed CKD stage 3–5 patients show enhanced ACE and decreased ACE2 expression on monocytes. This constellation renders the cells endothelial adhesive and likely supports the development of atherosclerosis.
authors.affiliation: Department of Internal Medicine II, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.
authors.forename: Bogusz
authors.initials: B
authors.lastname: Trojanowicz
authors.affiliation: Department of Internal Medicine II, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.
authors.forename: Christof
authors.initials: C
authors.lastname: Ulrich
authors.affiliation: Department of Internal Medicine II, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.
authors.forename: Felix
authors.initials: F
authors.lastname: Kohler
authors.affiliation: Department of Internal Medicine II, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.
authors.forename: Veronika
authors.initials: V
authors.lastname: Bode
authors.affiliation: Department of Internal Medicine II, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.
authors.forename: Eric
authors.initials: E
authors.lastname: Seibert
authors.affiliation: Department of Internal Medicine II, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.
authors.forename: Roman
authors.initials: R
authors.lastname: Fiedler
authors.affiliation: Department of Internal Medicine II, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Girndt
mesh_terms: D000368:Aged; D000085962:Angiotensin-Converting Enzyme 2; D050197:Atherosclerosis / Q000175:diagnosis* / Q000201:enzymology / Q000209:etiology; D016022:Case-Control Studies; D002465:Cell Movement; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000201:enzymology*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D000090063:Proto-Oncogene Mas; D051436:Renal Insufficiency, Chronic / Q000150:complications*
chemical_list: C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D007703:Peptidyl-Dipeptidase A; C000705307:ACE2 protein, human; D000085962:Angiotensin-Converting Enzyme 2
keywords: ACE2; atherosclerosis; CKD; monocytes; THP-1; uremia