title: Use of human methylation arrays for epigenome research in the common marmoset (Callithrix jacchus).
abstract: We examined the usefulness of commercially available DNA methylation arrays designed for the human genome (Illumina HumanMethylation450 and MethylationEPIC) for high-throughput epigenome analysis of the common marmoset, a nonhuman primate suitable for research on neuropsychiatric disorders. From among the probes on the methylation arrays, we selected those available for the common marmoset. DNA methylation data were obtained from genomic DNA extracted from the frontal cortex and blood samples of adult common marmosets as well as the frontal cortex of neonatal marmosets. About 10% of the probes on the arrays were estimated to be useful for DNA methylation assay in the common marmoset. Strong correlations existed between human and marmoset DNA methylation data. Illumina methylation arrays are useful for epigenome research using the common marmoset.
authors.affiliation: Laboratory for Molecular Dynamics of Mental Disorders, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako City, Saitama, 351-0198, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Ueda
authors.affiliation: Department of Neuropsychiatry, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan; Department of Molecular Brain Science, Graduate School of Life Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto City, Kumamoto, 860-8556, Japan.
authors.forename: Yui
authors.initials: Y
authors.lastname: Murata
authors.affiliation: Department of Molecular Brain Science, Graduate School of Life Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto City, Kumamoto, 860-8556, Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Bundo
authors.affiliation: Department of Functional Brain Imaging, National Institute of Radiological Sciences, National Institute for Quantum and Radiological Sciences and Technology, 4-9-1 Anagawa, Inage-ku, Chiba-shi, Chiba, 263-8555, Japan.
authors.forename: Arata
authors.initials: A
authors.lastname: Oh-Nishi
authors.affiliation: Laboratory of Animal Resources, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Kassai
authors.affiliation: Department of Neuropsychiatry, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
authors.forename: Tempei
authors.initials: T
authors.lastname: Ikegame
authors.affiliation: Department of Neuropsychiatry, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
authors.forename: Zhilei
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Neuropsychiatry, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Jinde
authors.affiliation: Laboratory of Animal Resources, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
authors.forename: Atsu
authors.initials: A
authors.lastname: Aiba
authors.affiliation: Department of Functional Brain Imaging, National Institute of Radiological Sciences, National Institute for Quantum and Radiological Sciences and Technology, 4-9-1 Anagawa, Inage-ku, Chiba-shi, Chiba, 263-8555, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Suhara
authors.affiliation: Department of Neuropsychiatry, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
authors.forename: Kiyoto
authors.initials: K
authors.lastname: Kasai
authors.affiliation: Laboratory for Molecular Dynamics of Mental Disorders, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako City, Saitama, 351-0198, Japan.
authors.forename: Tadafumi
authors.initials: T
authors.lastname: Kato
authors.affiliation: Department of Molecular Brain Science, Graduate School of Life Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto City, Kumamoto, 860-8556, Japan. Electronic address: iwamotok@kumamoto-u.ac.jp.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Iwamoto
mesh_terms: D000818:Animals; D002144:Callithrix; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D057890:Epigenomics / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008297:Male; D013045:Species Specificity
keywords: Beadarray; Cytosine modification; DNA methylation; Epigenetics; Nonhuman primate