title: Morphological and molecular characterization of an uninucleated cyst-producing Entamoeba spp. in captured Rangeland goats in Western Australia.
abstract: Uninucleated Entamoeba cysts measuring 7.3×7.7μm were detected in faecal samples collected from wild Rangeland goats (Capra hircus) after arrival at a commercial goat depot near Geraldton, Western Australia at a prevalence of 6.4% (8/125). Sequences were obtained at the 18S rRNA (n=8) and actin (n=5) loci following PCR amplification. At the 18S locus, phylogenetic analysis grouped the isolates closest with an E. bovis isolate (FN666250) from a sheep from Sweden with 99% similarity. At the actin locus, no E. bovis sequences were available, and the isolates shared 94.0% genetic similarity with E. suis from a pig in Western Japan. This is the first report to describe the morphology and molecular characterisation of Entamoeba from Rangeland goats in Western Australia and the first study to produce actin sequences from E. bovis-like Entamoeba sp.
authors.affiliation: School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, 6150, Australia.
authors.forename: Khalid
authors.initials: K
authors.lastname: Al-Habsi
authors.affiliation: School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, 6150, Australia.
authors.forename: Rongchang
authors.initials: R
authors.lastname: Yang
authors.affiliation: School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, 6150, Australia. Electronic address: Una.Ryan@murdoch.edu.au.
authors.forename: Una
authors.initials: U
authors.lastname: Ryan
authors.affiliation: School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, 6150, Australia.
authors.forename: Caroline
authors.initials: C
authors.lastname: Jacobson
authors.affiliation: School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, 6150, Australia.
authors.forename: David W
authors.initials: DW
authors.lastname: Miller
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004747:Entamoeba / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification; D004749:Entamoebiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D005243:Feces / Q000469:parasitology; D015511:Goat Diseases / Q000453:epidemiology / Q000469:parasitology*; D006041:Goats; D008297:Male; D033661:Oocysts; D010802:Phylogeny; D015995:Prevalence; D015800:Protozoan Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000662:veterinary; D014904:Western Australia / Q000453:epidemiology
chemical_list: D000199:Actins; D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D015800:Protozoan Proteins
keywords: 18S rRNA; Actin gene; Entamoeba; Rangeland goats