title: Growth hormone modulates in vitro endothelial cell migration and formation of capillary-like structures.
abstract: The generation of new blood vessels is a complex process mediated by a variety of growth factors, and the growth hormone (GH) has been shown to act as a proangiogenic factor. In fact, human GH deficiency or excess are associated with endothelial dysfunction. Moreover, mouse models have revealed the action of GH in both tissue repair and in the microvascular circulation of normal tissues. In this study, we investigated the in vitro effects of GH on endothelial cells. Using a murine endothelioma cell line (tEnd.1), we demonstrated that GH has a mitogenic effect. The hormone also affected the endothelial cellular morphology and augmented the deposition of the extracellular matrix molecules, laminin, and fibronectin, on tEnd.1 surface. GH could stimulate tEnd.1 cell fugetaxis, in transwell chambers migration assay, and increased the formation of capillary-like structures in Matrigel®-coated plates. Given the important role of angiogenesis during tissue injury, for example, at ischemic lesions, these findings shed light on therapeutic angiogenesis, particularly in pathologies where the cardiovascular system has been compromised.
authors.affiliation: Laboratory of Cell Biology, Institute of Biology and Health Science, Federal University of Alagoas, Maceió, Alagoas, Brazil.
authors.forename: Cícero Fagner
authors.initials: CF
authors.lastname: Messias de Lima
authors.affiliation: Laboratory of Cell Biology, Institute of Biology and Health Science, Federal University of Alagoas, Maceió, Alagoas, Brazil.
authors.forename: Maria Danielma
authors.initials: MD
authors.lastname: Dos Santos Reis
authors.forename: Fernando Wagner
authors.initials: FW
authors.lastname: da Silva Ramos
authors.affiliation: Laboratory of Cell Biology, Institute of Biology and Health Science, Federal University of Alagoas, Maceió, Alagoas, Brazil.
authors.forename: Silvana
authors.initials: S
authors.lastname: Ayres-Martins
authors.affiliation: Laboratory of Cell Biology, Institute of Biology and Health Science, Federal University of Alagoas, Maceió, Alagoas, Brazil.
authors.forename: Salete
authors.initials: S
authors.lastname: Smaniotto
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology; D004338:Drug Combinations; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D013006:Growth Hormone / Q000494:pharmacology*; D007797:Laminin / Q000494:pharmacology; D051379:Mice; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D011509:Proteoglycans / Q000494:pharmacology; D011986:Receptors, Somatotropin / Q000378:metabolism
chemical_list: D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; D011986:Receptors, Somatotropin; C059485:matrigel; D013006:Growth Hormone; D003094:Collagen
keywords: angiogenesis; cell migration; cell morphology; endothelium; growth factors; growth hormone