title: Draft genome sequence of reduced teicoplanin-susceptible and vancomycin-heteroresistant methicillin-resistant Staphylococcus aureus from sepsis cases.
abstract: Here we report the whole-genome shotgun sequence of six methicillin-resistant Staphylococcus aureus (MRSA) showing reduced susceptibility to both vancomycin and teicoplanin. The typical Indian community-acquired MRSA (CA-MRSA) clone ST772-MRSA-V-t657 was the most common genotype (3/6; 50%), followed by ST672-MRSA-IV (2/6; 33%) and ST22-MRSA-IV (1/6; 17%). All strains harboured a mutation in the tcaRAB operon, vraSR, graSR and/or rpoB genes, which are frequently mutated determinants in a heteroresistant vancomycin-intermediate S. aureus (hVISA) phenotype.
authors.affiliation: Department of Clinical Microbiology, Christian Medical College, Vellore 632004, Tamil Nadu, India.
authors.forename: Yamuna Devi
authors.initials: YD
authors.lastname: Bakthavatchalam
authors.affiliation: Department of Clinical Microbiology, Christian Medical College, Vellore 632004, Tamil Nadu, India. Electronic address: vbalaji@cmcvellore.ac.in.
authors.forename: Balaji
authors.initials: B
authors.lastname: Veeraraghavan
authors.affiliation: Department of Clinical Microbiology, Christian Medical College, Vellore 632004, Tamil Nadu, India.
authors.forename: Naveen Kumar
authors.initials: NK
authors.lastname: Devanga Ragupathi
authors.affiliation: Department of Clinical Microbiology, Christian Medical College, Vellore 632004, Tamil Nadu, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Babu
authors.affiliation: Department of Clinical Microbiology, Christian Medical College, Vellore 632004, Tamil Nadu, India.
authors.forename: Elakkiya
authors.initials: E
authors.lastname: Munuswamy
authors.affiliation: Department of Medicine (Unit II), Christian Medical College, Vellore 632004, India.
authors.forename: Thambu
authors.initials: T
authors.lastname: David
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D019295:Computational Biology; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D018805:Sepsis / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000382:microbiology*; D017334:Teicoplanin / Q000494:pharmacology; D014640:Vancomycin / Q000494:pharmacology; D020713:Vancomycin Resistance; D000073336:Whole Genome Sequencing
chemical_list: D000900:Anti-Bacterial Agents; D017334:Teicoplanin; D014640:Vancomycin
keywords: MRSA; hVISA; tcaA