title: Comprehensive understanding of PM2.5on gene and microRNA expression patterns in zebrafish (Danio rerio) model.
abstract: PM2.5is a major public health concern and some severe diseases have been attributed to exposure to PM2.5. However, a comprehensive understanding of gene and microRNA expression patterns induced by PM2.5is missing. The objective of this study was to evaluate the toxicity of PM2.5via genome-wide transcriptional analysis in the model teleost fish, zebrafish (Danio rerio). Gene ontology analysis revealed that the most impact gene functional categories induced by PM2.5included oxidation-reduction process, transport, response to xenobiotic stimulus, response to chemical stimulus and metabolic process. Pathway and Signal-net analysis showed that the critical pathway involved in the response to exposure to PM2.5was the metabolism of xenobiotics by cytochrome P450. Results from verification experiments also demonstrated that the key genes with degree higher than 10 induced by PM2.5were related to metabolism of xenobiotics by cytochrome P450, including cyp3a65, mgst2, gstp1, gsto2, gsto1, cyp1a, ehx1, gstal and aldh3b1. The differential expression of 8 microRNAs corresponding to those in the human genome, revealed that PM2.5could up-regulate let-7b, miR-153b-3p, miR-122, miR-24 and down-regulate let-7i, miR-19a-3p, miR-19b-3p and miR-7a, which suggested PM2.5had multiple means through which it induced toxicity in living organisms, such as suppression of adaptive immune responses, autophagy, deregulation of metabolism, impaired vasorelaxation, progression of cancers, as well as hypertension, atherosclerosis and myocardial infarction.
authors.affiliation: Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing, PR China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, PR China.
authors.forename: Junchao
authors.initials: J
authors.lastname: Duan
authors.affiliation: Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing, PR China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing, PR China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing, PR China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Jing
authors.affiliation: Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing, PR China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, PR China.
authors.forename: Man
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing, PR China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, PR China.
authors.forename: Ji
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing, PR China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, PR China.
authors.forename: Yanbo
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing, PR China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, PR China.
authors.forename: Xianqing
authors.initials: X
authors.lastname: Zhou
authors.affiliation: University/BHF Centre for Cardiovascular Science, Queens Medical Research Institute, The University of Edinburgh, Edinburgh, UK.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Miller
authors.affiliation: Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing, PR China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, PR China. Electronic address: zwsun@ccmu.edu.cn.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Sun
mesh_terms: D000818:Animals; D015870:Gene Expression / Q000187:drug effects; D035683:MicroRNAs / Q000235:genetics*; D052638:Particulate Matter / Q000009:adverse effects*; D015262:Xenobiotics / Q000378:metabolism; D015027:Zebrafish
chemical_list: D035683:MicroRNAs; D052638:Particulate Matter; D015262:Xenobiotics
keywords: Air pollution; Bioinformatics; Microarray; PM(2.5); Zebrafish; microRNA