title: Vascular endothelial growth factor-transfected adipose-derived stromal cells enhance bone regeneration and neovascularization from bone marrow stromal cells.
abstract: Vascular endothelial growth factor (VEGF)-transfected adipose-derived stromal cells (ADSCsVEGF) were devised to promote bone regeneration and neovascularization of bone marrow stromal cells (BMSCs). ADSCsVEGFwere added to BMSCs and cocultured in variable proportions. ADSCsVEGFalone or ADSCsVEGFwith BMSCs (BMSCs:ADSCsVEGFratio of 1:0.025-0.5) induced significantly greater tube formation in human umbilical vein endothelial cells than untransfected ADSCs. The cocultures of BMSCs and ADSCsVEGFat ratios of 1: 0.025-0.1 showed significantly greater osteogenic differentiation and mineralization than BMSCs alone in vitro. Osteogenic markers COL1A1, OCN and BSP were most effectively induced at the BMSC: ADSCVEGFratio of 1:0.05. Of angiogenesis-related genes, upregulation of cathepsin Z and downregulation of early growth response 1 were observed while two osteogenesis-related genes, osteoactivin and tetranectin, were upregulated in BMSCs/ADSCsVEGFcompared to BMSCs/ADSCs. When critical size calvarial defects in rats were implanted with mixture of BMSCs and ADSCsVEGFalong with hydroxyapatite/β-tricalcium phosphate granules, BMSCs and ADSCsVEGFat the ratio of 1:0.05 showed better bone regeneration that BMSCs alone. The cotransplantation of ADSCsVEGFwith BMSCs significantly increased neovascularization on the regenerated bone of the repaired defect than BMSCs alone. In conclusion, ADSCsVEGFadded in small proportion to BMSCs effectively promote bone regeneration and neovascularization. Copyright © 2017 John Wiley & Sons, Ltd.
authors.affiliation: Department of Orthopedics, Dongguk University Ilsan Hospital, Goyang, Korea.
authors.forename: Mi-Lan
authors.initials: ML
authors.lastname: Kang
authors.affiliation: Department of Orthopedics, Dongguk University Ilsan Hospital, Goyang, Korea.
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Kim
authors.affiliation: Department of Orthopedics, Dongguk University Ilsan Hospital, Goyang, Korea.
authors.forename: Gun-Il
authors.initials: GI
authors.lastname: Im
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D000368:Aged; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008875:Middle Aged; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D020411:Oligonucleotide Array Sequence Analysis; D010012:Osteogenesis / Q000187:drug effects / Q000235:genetics; D051381:Rats; D012886:Skull / Q000473:pathology; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*
chemical_list: D042461:Vascular Endothelial Growth Factor A; D000469:Alkaline Phosphatase
keywords: adipose derived stromal cells; bone marrow stromal cells; bone regeneration; cotransplantation; neovascularization; vascular endothelial growth factor