title: Defense Responses in Grapevine (cv. Mourvèdre) after Inoculation with the Botryosphaeria Dieback Pathogens Neofusicoccum parvum and Diplodia seriata and Their Relationship with Flowering.
abstract: As a result of the increasing economic impact of grapevine trunk diseases on viticulture worldwide, efficient and viable control strategies are urgently needed. However, understanding both plant-pathogen interactions and plant physiological changes related to these diseases is fundamental to such an achievement. In this study, we analyzed the effect of inoculation with the Botryosphaeria dieback fungal agents,Neofusicoccum parvumandDiplodia seriata, with and without inflorescence removal at the onset of G stage (separated clusters), I stage (flowering) and M stage (veraison). A measure of lesion size and real-time reverse-transcription polymerase chain reaction-based analysis were carried out. The results clearly show the importance of inflorescences in the development of lesions associated with Botryosphaeria dieback pathogens inoculated on green stems of adult vines, especially at the onset of flowering. At flowering, the biggest necroses were observed with the inflorescences present, as well as an activation of the studied defense responses. Thus, an ineffective response to the pathogen could be consistent with a possible metabolic reprogramming linked to the host phenophase.
authors.affiliation: Structure Fédérative de Recherche (SFR) Condorcet-FR CNRS 3417, Université de Reims Champagne-Ardenne, Unité de Recherche Vignes et Vins de Champagne EA 4707, Laboratoire Stress, Défenses et Reproduction des Plantes, BP 1039, (Cedex 2), 51687 Reims, France. alessandro.spagnolo@univ-reims.fr.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Spagnolo
authors.affiliation: Structure Fédérative de Recherche (SFR) Condorcet-FR CNRS 3417, Université de Reims Champagne-Ardenne, Unité de Recherche Vignes et Vins de Champagne EA 4707, Laboratoire Stress, Défenses et Reproduction des Plantes, BP 1039, (Cedex 2), 51687 Reims, France. vincenzo.mondello@univ-reims.fr.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Mondello
authors.affiliation: Institut Français de la Vigne et du Vin Pôle Rhône-Méditerranée, 7 Avenue Cazeaux, 30230 Rodilhan, France. philippe.larignon@vignevin.com.
authors.forename: Philippe
authors.initials: P
authors.lastname: Larignon
authors.affiliation: Structure Fédérative de Recherche (SFR) Condorcet-FR CNRS 3417, Université de Reims Champagne-Ardenne, Unité de Recherche Vignes et Vins de Champagne EA 4707, Laboratoire Stress, Défenses et Reproduction des Plantes, BP 1039, (Cedex 2), 51687 Reims, France. sandra.villaume@univ-reims.fr.
authors.forename: Sandra
authors.initials: S
authors.lastname: Villaume
authors.affiliation: Structure Fédérative de Recherche (SFR) Condorcet-FR CNRS 3417, Université de Reims Champagne-Ardenne, Unité de Recherche Vignes et Vins de Champagne EA 4707, Laboratoire Stress, Défenses et Reproduction des Plantes, BP 1039, (Cedex 2), 51687 Reims, France. clarisse.rabenoelina@univ-reims.fr.
authors.forename: Fanja
authors.initials: F
authors.lastname: Rabenoelina
authors.affiliation: Structure Fédérative de Recherche (SFR) Condorcet-FR CNRS 3417, Université de Reims Champagne-Ardenne, Unité de Recherche Vignes et Vins de Champagne EA 4707, Laboratoire Stress, Défenses et Reproduction des Plantes, BP 1039, (Cedex 2), 51687 Reims, France. christophe.clement@univ-reims.fr.
authors.forename: Christophe
authors.initials: C
authors.lastname: Clément
authors.affiliation: Structure Fédérative de Recherche (SFR) Condorcet-FR CNRS 3417, Université de Reims Champagne-Ardenne, Unité de Recherche Vignes et Vins de Champagne EA 4707, Laboratoire Stress, Défenses et Reproduction des Plantes, BP 1039, (Cedex 2), 51687 Reims, France. florence.fontaine@univ-reims.fr.
authors.forename: Florence
authors.initials: F
authors.lastname: Fontaine
mesh_terms: D001203:Ascomycota; D060467:Disease Resistance; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D054884:Host-Pathogen Interactions; D010935:Plant Diseases / Q000382:microbiology*; D027843:Vitis / Q000382:microbiology* / Q000502:physiology*
keywords: Botryosphaeria dieback; Diplodia seriata; Neofusicoccum parvum; defense responses; flowering