title: Magnetic lateral flow immunoassay test strip development - Considerations for proof of concept evaluation.
abstract: Lateral flow immunoassays (LFIA) have grown to become the predominant test device format for the diagnostics and point-of-care industries. The demand for robust and reproducible LFIAs has been facilitated through scale-up production methods using specialized and automated instruments. However, the feasibility of a LFIA device can still be evaluated in a small-scale laboratory setting through controlled manual preparation methods. The advent of super-paramagnetic (SPMP) labels for use in lateral flow has heralded the possibility of highly sensitive and stable LFIAs. The methods used for the preparation of a magnetic LFIA prototype device using a reserved suite of laboratory equipment are described.
authors.affiliation: School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland.
authors.forename: R
authors.initials: R
authors.lastname: Connolly
authors.affiliation: School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland. Electronic address: richard.okennedy@dcu.ie.
authors.forename: R
authors.initials: R
authors.lastname: O' Kennedy
mesh_terms: D000906:Antibodies / Q000652:urine*; D000941:Antigens / Q000737:chemistry*; D003101:Collodion; D052203:Ferrosoferric Oxide / Q000737:chemistry; D006801:Humans; D007118:Immunoassay; D018796:Immunoconjugates / Q000737:chemistry*; D060526:Magnetic Fields; D059346:Magnets; D008567:Membranes, Artificial; D019095:Point-of-Care Systems; D000075082:Proof of Concept Study; D011934:Reagent Strips; D012212:Rheology
chemical_list: D000906:Antibodies; D000941:Antigens; D018796:Immunoconjugates; D008567:Membranes, Artificial; D011934:Reagent Strips; D003101:Collodion; D052203:Ferrosoferric Oxide
keywords: Lateral flow immunoassay; super-paramagnetic particles