title: Acidobacteria Community Responses to Nitrogen Dose and Form in Chinese Fir Plantations in Southern China.
abstract: Acidobacteria is a new bacterial group, identified by molecular research, which is widely distributed and has specific ecological functions in forest soil. In this study, we investigated Acidobacteria response to N input, and the effects were related to N form and dose. The experimental design included two N forms (NH4+-N and NO3--N) and five levels of N deposition (0, 20, 40, 60, 80 kg N ha-1) for 2 years. Research into the Acidobacteria community was conducted using 16Sr RNA gene-based high-throughput pyrosequencing methods. Acidobacteria OTUs and N had a negative relationship in 0-60 kg ha-1year-1; however, at N doses beyond a certain size, nitrogen might promote an increase in Acidobacteria OTUs. The Acidobacteria relative abundance under NH4+-N treatment was higher than under NO3--N treatment. Acidobacteria relative abundance decreased with increasing of NH4+-N dose, but increased with increasing NO3--N dose. Overall, 13 different Acidobacteria subgroups were identified, with Gp1, Gp2, and Gp3 being dominant. Significant differences in Acidobacteria distribution were primarily caused by N input and pH value. The environmental factors of N were all negatively related to Acidobacteria distribution in low N dose treatments (0-20 kg ha-1year-1), but were positively related in response to N dose treatments (40-80 kg ha-1year-1).
authors.affiliation: Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, People's Republic of China.|State Key Laboratory of Tree Genetics and Breeding, Beijing, 100091, People's Republic of China.|Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Beijing, 100091, People's Republic of China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Liu
authors.affiliation: Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, People's Republic of China.|State Key Laboratory of Tree Genetics and Breeding, Beijing, 100091, People's Republic of China.|Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Beijing, 100091, People's Republic of China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, People's Republic of China.|State Key Laboratory of Tree Genetics and Breeding, Beijing, 100091, People's Republic of China.|Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Beijing, 100091, People's Republic of China.
authors.forename: Lingyu
authors.initials: L
authors.lastname: Hou
authors.affiliation: Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, People's Republic of China.|State Key Laboratory of Tree Genetics and Breeding, Beijing, 100091, People's Republic of China.|Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Beijing, 100091, People's Republic of China.
authors.forename: Nan
authors.initials: N
authors.lastname: Deng
authors.affiliation: Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, People's Republic of China. jiaorzh@caf.ac.cn.|State Key Laboratory of Tree Genetics and Breeding, Beijing, 100091, People's Republic of China. jiaorzh@caf.ac.cn.|Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Beijing, 100091, People's Republic of China. jiaorzh@caf.ac.cn.
authors.forename: Ruzhen
authors.initials: R
authors.lastname: Jiao
mesh_terms: D061271:Acidobacteria / Q000378:metabolism*; D064751:Ammonium Compounds / Q000378:metabolism; D002681:China; D065928:Forests; D009566:Nitrates / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
chemical_list: D064751:Ammonium Compounds; D009566:Nitrates; D012987:Soil; D009584:Nitrogen
keywords: Acidobacteria; Ammonium; Forest soil; N dose; Nitrate; Subgroup