title: Biocompatible chitosan nanoparticles as an efficient delivery vehicle for Mycobacterium tuberculosis lipids to induce potent cytokines and antibody response through activation of γδ T cells in mice.
abstract: The activation of cell-mediated and humoral immune responses to Mycobacterium tuberculosis (Mtb) is critical for protection against the pathogen and nanoparticle-mediated delivery of antigens is a more potent way to induce different immune responses. Herein, we show that mice immunized with Mtb lipid-bound chitosan nanoparticles (NPs) induce secretion of prominent type-1 T-helper (Th-1) and type-2 T-helper (Th-2) cytokines in lymph node and spleen cells, and also induces significantly higher levels of IgG, IgG1, IgG2 and IgM in comparison to control mice. Furthermore, significantly enhanced γδ-T-cell activation was observed in lymph node cells isolated from mice immunized with Mtb lipid-coated chitosan NPs as compared to mice immunized with chitosan NPs alone or Mtb lipid liposomes. In comparison to CD8+cells, significantly higher numbers of CD4+cells were present in both the lymph node and spleen cells isolated from mice immunized with Mtb lipid-coated chitosan NPs. In conclusion, this study represents a promising new strategy for the efficient delivery of Mtb lipids using chitosan NPs to trigger an enhanced cell-mediated and antibody response against Mtb lipids.
authors.affiliation: School of Biotechnology, KIIT University, Bhubaneswar, Odisha, India.
authors.forename: Ishani
authors.initials: I
authors.lastname: Das
authors.forename: Avinash
authors.initials: A
authors.lastname: Padhi
authors.forename: Sitabja
authors.initials: S
authors.lastname: Mukherjee
authors.forename: Debi P
authors.initials: DP
authors.lastname: Dash
authors.forename: Santosh
authors.initials: S
authors.lastname: Kar
authors.forename: Avinash
authors.initials: A
authors.lastname: Sonawane
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D048271:Chitosan / Q000737:chemistry; D016207:Cytokines / Q000378:metabolism*; D004705:Endocytosis / Q000502:physiology; D005260:Female; D006801:Humans; D056724:Immunity, Humoral / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D000075942:Intraepithelial Lymphocytes / Q000187:drug effects / Q000276:immunology*; D008055:Lipids / Q000008:administration & dosage*; D017737:Macrophages, Peritoneal / Q000187:drug effects; D008807:Mice, Inbred BALB C; D009169:Mycobacterium tuberculosis / Q000737:chemistry*; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D014397:Tuberculosis, Pulmonary / Q000188:drug therapy / Q000276:immunology
chemical_list: D001672:Biocompatible Materials; D016207:Cytokines; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008055:Lipids; D048271:Chitosan