title: MiR-124-3p attenuates hyperphosphorylation of Tau protein-induced apoptosis via caveolin-1-PI3K/Akt/GSK3β pathway in N2a/APP695swe cells.
abstract: Hyperphosphorylation of Tau forming neurofibrillary tangles has been considered as a crucial event in the pathogenesis of Alzheimer's disease (AD). MiR-124-3p belongs to microRNA (miRNA) family and was markedly decreased in AD, however, the functions of miR-124-3p in the pathogenesis of AD remain unknown. We observed that the expression of miR-124-3p was significantly decreased in N2a/APP695swe cells; and transfection of miR-124-3p mimics not only attenuated cell apoptosis and abnormal hyperphosphorylation of Tau protein without any changes of total Tau protein, but also increased expression levels of Caveolin-1, phosphoinositide 3-kinase (PI3K), phospho-Akt (Akt-Ser473)/Akt, phospho-glycogen synthase kinase-3 beta (GSK-3β-Ser9)/GSK-3β in N2a/APP695swe cells. We further found that miR-12-3p directly targeted Caveolin-1; miR-124-3p inhibited abnormal hyperphosphorylation of Tau by regulating Caveolin-1-PI3K/Akt/GSK3β pathway in AD. This study reveals that miR-124-3p may play a neuroprotective role in AD, which may provide new ideas and therapeutic targets for AD.
authors.affiliation: Department of Pathology, Chongqing Medical University, Chongqing, 400016, China.|Center for Molecular Medicine Testing, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Qingmei
authors.initials: Q
authors.lastname: Kang
authors.affiliation: Department of Pathology, Chongqing Medical University, Chongqing, 400016, China.|Center for Molecular Medicine Testing, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Xiang
authors.affiliation: Department of Pathology, Chongqing Medical University, Chongqing, 400016, China.|Center for Molecular Medicine Testing, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Pathology, Chongqing Medical University, Chongqing, 400016, China.|Center for Molecular Medicine Testing, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liang
authors.affiliation: Center for Molecular Medicine Testing, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Chongqing Medical University, Chongqing, 400016, China.|Center for Molecular Medicine Testing, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Fanlin
authors.initials: F
authors.lastname: Zhou
authors.affiliation: Department of Pathology, Chongqing Medical University, Chongqing, 400016, China.|Center for Molecular Medicine Testing, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Mengyuan
authors.initials: M
authors.lastname: Qiao
authors.affiliation: Department of Pathology, Chongqing Medical University, Chongqing, 400016, China.|Center for Molecular Medicine Testing, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Yingling
authors.initials: Y
authors.lastname: Nie
authors.affiliation: Department of Pathology, Chongqing Medical University, Chongqing, 400016, China.|Center for Molecular Medicine Testing, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Yurong
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Pathology, Chongqing Medical University, Chongqing, 400016, China.|Center for Molecular Medicine Testing, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Jingyi
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Department of Neurosurgery, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, 550004, China.
authors.forename: Yubing
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Department of Pathology, Chongqing Medical University, Chongqing, 400016, China.|Center for Molecular Medicine Testing, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D051242:Caveolin 1 / Q000378:metabolism*; D045744:Cell Line, Tumor; D015536:Down-Regulation; D000071679:Glycogen Synthase Kinase 3 beta / Q000378:metabolism*; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D009447:Neuroblastoma; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D010766:Phosphorylation; D015398:Signal Transduction; D014162:Transfection; D016875:tau Proteins / Q000378:metabolism*
chemical_list: D051242:Caveolin 1; D035683:MicroRNAs; C540079:Mirn124 microRNA, mouse; D016875:tau Proteins; D019869:Phosphatidylinositol 3-Kinases; D000071679:Glycogen Synthase Kinase 3 beta
keywords: Alzheimer's disease; PI3K/Akt/GSK3β; Tau; caveolin-1; miR-124-3p