title: Antimicrobial Effect of Biocompatible Silicon Nanoparticles Activated Using Therapeutic Ultrasound.
abstract: In this study, we report a method for the suppression of Escherichia coli (E. coli) vitality by means of therapeutic ultrasound irradiation (USI) using biocompatible silicon nanoparticles as cavitation sensitizers. Silicon nanoparticles without (SiNPs) and with polysaccharide (dextran) coating (DSiNPs) were used. Both types of nanoparticles were nontoxic to Hep 2 cells up to a concentration of 2 mg/mL. The treatment of bacteria with nanoparticles and application of 1 W/cm2USI resulted in the reduction of their viabilities up to 35 and 72% for SiNPs and DSiNPs, respectively. The higher bacterial viability reduction for DSiNPs as compared with SiNPs can be explained by the fact that the biopolymer shell of the polysaccharide provides a stronger adhesion of nanoparticles to the bacterial surface. Transmission electron microscopy (TEM) studies showed that the bacterial lipid shell was partially perforated after the combined treatment of DSiNPs and USI, which can be explained by the lysis of bacterial membrane due to the cavitation sensitized by the SiNPs. Furthermore, we have shown that 100% inhibition of E. coli bacterial colony growth is possible by coupling the treatments of DSiNPs and USI with an increased intensity of up to 3 W/cm2. The observed results reveal the application of SiNPs as promising antimicrobial agents.
authors.forename: Svetlana N
authors.initials: SN
authors.lastname: Shevchenko
authors.affiliation: Leibniz Institute of Photonic Technology , Albert Einstein Street 9, D-07745 Jena, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Burkhardt
authors.forename: Eugene V
authors.initials: EV
authors.lastname: Sheval
authors.forename: Ulyana A
authors.initials: UA
authors.lastname: Natashina
authors.affiliation: Leibniz Institute of Photonic Technology , Albert Einstein Street 9, D-07745 Jena, Germany.|Center for Sepsis Control and Care, Jena University Hospital , Erlanger Allee 101, 07747 Jena, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Grosse
authors.forename: Alexander L
authors.initials: AL
authors.lastname: Nikolaev
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Gopin
authors.affiliation: Leibniz Institute of Photonic Technology , Albert Einstein Street 9, D-07745 Jena, Germany.|Center for Sepsis Control and Care, Jena University Hospital , Erlanger Allee 101, 07747 Jena, Germany.
authors.forename: Ute
authors.initials: U
authors.lastname: Neugebauer
authors.affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Science , Pushino 142290, Russian Federation.
authors.forename: Andrew A
authors.initials: AA
authors.lastname: Kudryavtsev
authors.affiliation: Leibniz Institute of Photonic Technology , Albert Einstein Street 9, D-07745 Jena, Germany.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Sivakov
authors.forename: Liubov A
authors.identifier: 0000-0001-7485-0495
authors.initials: LA
authors.lastname: Osminkina
mesh_terms: D000890:Anti-Infective Agents; D004926:Escherichia coli; D053758:Nanoparticles; D012825:Silicon; D012822:Silicon Dioxide
chemical_list: D000890:Anti-Infective Agents; D012822:Silicon Dioxide; D012825:Silicon