title: Evaluation of vascular tone and cardiac contractility in response to silver nanoparticles, using Langendorff rat heart preparation.
abstract: Silver nanoparticles (AgNPs) have been widely used because of their antimicrobial properties. However, several reports suggest that AgNPs exposure promote cardiac effects that involve nitric oxide (NO) and oxidative stress (OS). Nevertheless, there are no studies related to AgNPs-induced effects in cardiac physiology. The aim of this study was to evaluate the AgNPs direct actions on coronary vascular tone and cardiac contractility using Langendorff rat heart preparation. Low concentrations of AgNPs (0.1 and 1 μg/mL) increased NO derived from inducible NO-synthase (iNOS), without modifying cardiac parameters. Meanwhile, high concentrations (10 and 100 μg/mL) promoted a sustained vasoconstriction and increased cardiac contractility related to OS, leading to rhabdomyolysis. Furthermore, AgNPs were internalized in the cardiac muscle, hindering classic actions induced by phenylephrine (Phe) and acetylcholine (ACh). These data suggest that AgNPs affect cardiac physiology in function of the concentration and in part of the NO generation, NOS expression and OS.
authors.affiliation: Facultad de Ciencias Quimicas, Universidad Autonoma de San Luis Potosi, San Luis Potosi, S.L.P., Mexico.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Ramirez-Lee Manuel
authors.affiliation: Facultad de Ciencias Quimicas, Universidad Autonoma de San Luis Potosi, San Luis Potosi, S.L.P., Mexico.
authors.forename: Pedro Pablo
authors.initials: PP
authors.lastname: Martinez-Cuevas
authors.affiliation: Facultad de Ciencias Quimicas, Universidad Autonoma de San Luis Potosi, San Luis Potosi, S.L.P., Mexico.
authors.forename: Hector
authors.initials: H
authors.lastname: Rosas-Hernandez
authors.affiliation: Departamento de Patologia, Hospital Central «Dr. Ignacio Morones Prieto», San Luis Potosi, S.L.P., Mexico.
authors.forename: Cuauhtémoc
authors.initials: C
authors.lastname: Oros-Ovalle
authors.affiliation: Instituto Potosino de Investigación Científica y Tecnológica, División de Materiales Avanzados, San Luis Potosi, S.L.P., Mexico.
authors.forename: Mariela
authors.initials: M
authors.lastname: Bravo-Sanchez
authors.affiliation: Facultad de Estomatologia, Universidad Autonoma de San Luis Potosi, San Luis Potosi, S.L.P., Mexico.
authors.forename: Gabriel Alejandro
authors.initials: GA
authors.lastname: Martinez-Castañon
authors.affiliation: Facultad de Ciencias Quimicas, Universidad Autonoma de San Luis Potosi, San Luis Potosi, S.L.P., Mexico. Electronic address: cgonzalez.uaslp@gmail.com.
authors.forename: Carmen
authors.initials: C
authors.lastname: Gonzalez
mesh_terms: D000818:Animals; D006321:Heart / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry*; D009119:Muscle Contraction / Q000187:drug effects*; D009569:Nitric Oxide / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D012834:Silver / Q000737:chemistry*; D014661:Vasoconstriction
chemical_list: D009569:Nitric Oxide; D012834:Silver; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat
keywords: Coronary vascular tone; Myocardial contractility; Nitric oxide; Oxidative stress; Silver nanoparticles