title: Gecko-Inspired Nanotentacle Surface-Enhanced Raman Spectroscopy Substrate for Sampling and Reliable Detection of Pesticide Residues in Fruits and Vegetables.
abstract: Rapid sampling and multicomponent detection are crucial for monitoring of pesticide residues analysis. Here, a gecko-inspired nanotentacle surface-enhanced Raman spectroscopy (G-SERS) platform is proposed for the first time for the simultaneous detection of three kinds of pesticides via a simple and intuitive "press and peeled-off" approach. The G-SERS platform obtained from seeding deposition of silver nanoparticles (Ag NPs) on 3D PDMS nanotentacle array is flexible and free-standing. Compared with other substrates, this G-SERS substrate can simultaneously provide outstanding SERS activity (enhancement factor = 1.2 × 107), superior reproducibility (RSD = 5.8%) and countless flexible nanoscale "tentacles" (∼6.7 × 108/cm2). Moreover, the high density of "tentacles" can freely approach the microarea and enable efficient target collection, which were confirmed by SEM and HPLC. By direct sampling from cucumber, apple, and grape surfaces, thiram (TMTD), methyl parathion (MPT), malachite green (MG), and their multiple components have been rapidly and reliably determined. For example, under the optimal conditions, a sensitivity of 1.6 ng/cm2(S/N = 3) for TMTD was obtained on apple peels with a correlation coefficient (R) of 0.99. Therefore, the G-SERS substrate could offer a great practical potential for on-spot identification of various pesticide residues on real samples.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Food Science and Technology, College of Science, Huazhong Agricultural University , Wuhan 430070, PR China.
authors.forename: Pan
authors.initials: P
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Food Science and Technology, College of Science, Huazhong Agricultural University , Wuhan 430070, PR China.
authors.forename: Long
authors.initials: L
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Food Science and Technology, College of Science, Huazhong Agricultural University , Wuhan 430070, PR China.
authors.forename: Zhicheng
authors.initials: Z
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Food Science and Technology, College of Science, Huazhong Agricultural University , Wuhan 430070, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Food Science and Technology, College of Science, Huazhong Agricultural University , Wuhan 430070, PR China.
authors.forename: Wenmin
authors.initials: W
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Food Science and Technology, College of Science, Huazhong Agricultural University , Wuhan 430070, PR China.
authors.forename: Fan
authors.initials: F
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Food Science and Technology, College of Science, Huazhong Agricultural University , Wuhan 430070, PR China.
authors.forename: Heyou
authors.identifier: 0000-0001-9406-0722
authors.initials: H
authors.lastname: Han
mesh_terms: D005638:Fruit / Q000737:chemistry / Q000378:metabolism; D027845:Malus / Q000737:chemistry* / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D008743:Methyl Parathion / Q000737:chemistry; D010573:Pesticide Residues / Q000032:analysis*; D015203:Reproducibility of Results; D012394:Rosaniline Dyes / Q000737:chemistry; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013893:Thiram / Q000737:chemistry; D014675:Vegetables / Q000737:chemistry* / Q000378:metabolism; D027843:Vitis / Q000737:chemistry / Q000378:metabolism
chemical_list: D010573:Pesticide Residues; D012394:Rosaniline Dyes; D013893:Thiram; C005095:malachite green; D012834:Silver; D008743:Methyl Parathion