title: Förster Resonance Energy Transfer Mediated Photoluminescence Quenching in Stoichiometrically Assembled CdSe/ZnS Quantum Dot-Peptide Labeled Black Hole Quencher Conjugates for Matrix Metalloproteinase-2 Sensing.
abstract: The steady state and time-resolved photoluminescence quenching of streptavidin modified CdSe/ZnS quantum dots (QDs) instigated by biotin-peptide-BHQ-1 (biotin-pep-BHQ-1) molecule was investigated. Here, we have achieved an efficient photoluminescence (PL) quenching of QDs with the conjugation of dark quencher (black hole quencher-BHQ) molecules intermediated with the GPLGVRGK peptide. The luminescence of streptavidin-QDs585 was decreased upon titration with a nano molar concentration of the biotin-GPLGVRGK-BHQ-1 molecule. It has been suggested that the decrease of QDs PL occurred through a Förster resonance energy transfer (FRET) mechanism from the analysis of steady state photoluminescence intensity measurements as well as time resolved lifetime measurements of streptavidin-QDs and QDs-(pep-BHQ-1)nconjugates. The sequence of intermediate peptide GPLG↓VRGK can act as a target material for matrix metalloproteinases-2 (MMP-2) produced by cancer cells at its Gly and Val region, shown by the down-headed arrow. Interestingly, here the reported self-assembled QDs-(pep-BHQ-1)n conjugates could detect the presence MMP-2 at a detection limit of 1 ng/mL with a clear luminescence recovery.
authors.affiliation: Graduate School of Engineering, Nagoya University.
authors.forename: Sreenadh Sasidharan
authors.initials: SS
authors.lastname: Pillai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yukawa
authors.forename: Daisuke
authors.initials: D
authors.lastname: Onoshima
authors.forename: Vasudevanpillai
authors.initials: V
authors.lastname: Biju
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Baba
mesh_terms: D019187:Cadmium Compounds / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D008163:Luminescent Measurements; D020778:Matrix Metalloproteinase 2 / Q000032:analysis* / Q000737:chemistry; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry*; D011487:Protein Conformation; D045663:Quantum Dots / Q000737:chemistry*; D018036:Selenium Compounds / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry*; D017967:Zinc Compounds / Q000737:chemistry*
chemical_list: D019187:Cadmium Compounds; D010455:Peptides; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; D019809:Streptavidin; C058667:cadmium selenide; D020778:Matrix Metalloproteinase 2; C031238:zinc sulfide