title: Genotoxicity evaluation of hydroalcoholic and aqueous extracts ofDorema aucheriby the comet assay.
abstract: BACKGROUND
  Dorema aucheriis a plant of Apiaceae family which is used widely in some states of Iran. Different extracts and essential oil ofDoremaspecies contain flavonoids and cumarin compounds which have anti-hypertensive, cholesterol- and triglycerides-lowering properties. This study was undertaken to evaluate the genotoxic properties of hydroalcoholic and aqueous extracts ofD. aucherion human hepatoma cells using the comet assay method for safety evaluation.
  
  
  METHODS
  In this method, after incubation of cells with different concentrations of extracts, cell suspensions were added to pre-coated normal agarose slides. After lysis, electrophoresis and neutralization process, staining was done by ethidium bromide and comets were observed using a fluorescence microscope. Tail length, percentage of DNA in tail and tail moment parameters were measured.
  
  
  RESULTS
  Statistical analysis of the results demonstrated that concentrations more than 500 μg/ml of hydroalcoholic and aqueous extract ofD. aucheriwere genotoxic.
  
  
  CONCLUSIONS
  It can be concluded from the results that taking the concentrations less than these dosages of extracts are safe but more studies are required to determine genotoxic mechanisms of this plant.
authors.affiliation: Department of Pharmacology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Etebari
authors.affiliation: Department of Pharmacognosy, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Seyed Ebrahim
authors.initials: SE
authors.lastname: Sajjadi
authors.affiliation: Department of Pharmacology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Jafarian-Dehkordi
authors.affiliation: Department of Pharmacology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Sajjad
authors.initials: S
authors.lastname: Nazmakanipour
keywords: Comet assay; Dorema aucheri; HepG2; genotoxicity