title: Using Digital Quantification of Stained Tissue Microarrays as a Medium-Throughput, Quantitative Method for Measuring the Kinetics of Signal Transduction.
abstract: Determining how a molecular targeting agent affects signaling pathways is challenging. To facilitate this exploration, we use a combination of medium-throughput methodologies such as tissue microarrays (TMA) and digital image analysis for quantification of immunohistochemistry (IHC). Because TMAs enable the simultaneous analysis of up to 1000 tissues, this tool can be used to comprehensively assess changes in signal transduction pathways over time. TMAs can be digitized and analyzed using the Aperio ScanScope imaging system and Aperio ImageScope software to deliver semiquantitative data. This chapter describes the methodologies for tissue collection and for the construction, sectioning, staining, and digital analysis of TMAs.
authors.affiliation: Department of Experimental Therapeutics, British Columbia Cancer Agency, 675 West 10th Avenue, 5th Floor, Vancouver, BC, V5Z 1L3, Canada. jessica.kalra@gmail.com.|Langara College, Vancouver, BC, Canada. jessica.kalra@gmail.com.|Department of Anesthesia Pharmacology, University of British Columbia, Vancouver, BC, Canada. jessica.kalra@gmail.com.
authors.forename: Jessica
authors.initials: J
authors.lastname: Kalra
authors.affiliation: Integrative Oncology, BC Cancer Agency, Vancouver, BC, Canada.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Baker
mesh_terms: D019295:Computational Biology / Q000379:methods; D057166:High-Throughput Screening Assays; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007150:Immunohistochemistry; D007700:Kinetics; D061848:Optical Imaging / Q000379:methods; D015398:Signal Transduction; D012984:Software; D046888:Tissue Array Analysis / Q000379:methods; D014584:User-Computer Interface
keywords: Aperio; Drug efficacy; Immunohistochemistry; Preclinical; Signal transduction; Targeted therapy; Tissue microarray