title: Quantitative assessment of the relationship between RASSF1A gene promoter methylation and bladder cancer (PRISMA).
abstract: BACKGROUND
  Methylation of the Ras-association domain family 1 isoform A (RASSF1A) gene promoter region is thought to participate in the initiation and development of many different cancers. However, in bladder cancer the role of RASSF1A methylation was unclear. To evaluate the relationship between RASSF1A methylation and bladder cancer, a quantitative assessment of an independent meta-analysis was performed. In addition, a DNA methylation microarray database from the cancer genome atlas (TCGA) project was used to validate the results of the meta-analysis.
  
  
  METHODS
  We searched published articles from computerized databases, and DNA methylation data were extracted from TCGA project. All data were analyzed by R software.
  
  
  RESULTS
  The results of the meta-analysis indicated that the frequency of RASSF1A gene methylation in bladder cancer patients is significantly higher than in healthy controls. The hazard ratio (HR) was 2.24 (95% CI = [1.45; 3.48], P = 0.0003) for overall survival (OS), and the RASSF1A gene promoter methylation status was strongly associated with the TNM stage and differentiation grade of the tumor. The similar results were also found by the data from TCGA project.
  
  
  CONCLUSIONS
  There was a significant relationship between the methylation of the RASSF1A gene promoter and bladder cancer. Therefore, RASSF1A gene promoter methylation will be a potential biomarker for the clinical diagnosis of bladder cancer.
authors.affiliation: Anesthesia department, People's Hospital of China Three Gorges University, The First People's Hospital of Yichang, Yichang, Hubei, P.R. China Ultrasound department, People's Hospital of China Three Gorges University, The First People's Hospital of Yichang, Yichang, Hubei, P.R. China Cardiothoracic surgery, People's Hospital of China Three Gorges University, The First People's Hospital of Yichang, Yichang, Hubei, P.R. China Department of science and education, People's Hospital of China Three Gorges University, The First People's Hospital of Yichang, Yichang, Hubei, P.R. China.
authors.forename: Leyun
authors.initials: L
authors.lastname: Zhan
authors.forename: Bingyi
authors.initials: B
authors.lastname: Zhang
authors.forename: Yaojun
authors.initials: Y
authors.lastname: Tan
authors.forename: Chengliang
authors.initials: C
authors.lastname: Yang
authors.forename: Chenhong
authors.initials: C
authors.lastname: Huang
authors.forename: Qiongya
authors.initials: Q
authors.lastname: Wu
authors.forename: Yulin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Chen
authors.forename: Mi
authors.initials: M
authors.lastname: Zhou
authors.forename: Aihua
authors.initials: A
authors.lastname: Shu
mesh_terms: D000367:Age Factors; D014408:Biomarkers, Tumor; D019175:DNA Methylation / Q000235:genetics; D006801:Humans; D009367:Neoplasm Staging; D011401:Promoter Regions, Genetic; D016016:Proportional Hazards Models; D012737:Sex Factors; D016019:Survival Analysis; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; C411762:RASSF1 protein, human; D025521:Tumor Suppressor Proteins