title: Mouse Microsurgery Infusion Technique for Targeted Substance Delivery into the CNS via the Internal Carotid Artery.
abstract: Animal models of central nervous system (CNS) diseases and, consequently, blood-brain barrier disruption diseases, require the delivery of exogenous substances into the brain. These exogenous substances may induce injurious impact or constitute therapeutic strategy. The most common delivery methods of exogenous substances into the brain are based on systemic deliveries, such as subcutaneous or intravenous routes. Although commonly used, these approaches have several limitations, including low delivery efficacy into the brain. In contrast, surgical methods that locally deliver substances into the CNS are more specific and prevent the uptake of the exogenous substances by other organs. Several surgical methods for CNS delivery are available; however, they tend to be very traumatic. Here, we describe a mouse infusion microsurgery technique, which effectively delivers substances into the brain via the internal carotid artery, with minimal trauma and no interference with normal CNS functionality.
authors.affiliation: Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami.
authors.forename: Ana R
authors.initials: AR
authors.lastname: Leda
authors.affiliation: Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami.
authors.forename: Levy
authors.initials: L
authors.lastname: Dygert
authors.affiliation: Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami.
authors.forename: Luc
authors.initials: L
authors.lastname: Bertrand
authors.affiliation: Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami; The Jerzy Kukuczka Academy of Physical Education; mtoborek@med.miami.edu.
authors.forename: Michal
authors.initials: M
authors.lastname: Toborek
mesh_terms: D000700:Analgesics / Q000008:administration & dosage; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage; D001812:Blood-Brain Barrier / Q000378:metabolism; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002343:Carotid Artery, Internal / Q000601:surgery*; D002493:Central Nervous System Diseases / Q000188:drug therapy / Q000473:pathology / Q000662:veterinary; D006678:HIV / Q000235:genetics; D007275:Injections, Intravenous; D051379:Mice; D008856:Microscopy, Fluorescence; D008866:Microsurgery; D004364:Pharmaceutical Preparations / Q000008:administration & dosage*; D012367:RNA, Viral / Q000008:administration & dosage
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D004364:Pharmaceutical Preparations; D012367:RNA, Viral