title: Genotypic and allelic frequencies of gene polymorphisms associated with meat tenderness in Nellore beef cattle.
abstract: The objectives of this study were to characterize the allelic and genotypic frequencies of polymorphisms in the µ-calpain and calpastatin genes, and to assess their association with meat tenderness and animal growth in Nellore cattle. We evaluated 605 Nellore animals at 24 months of age, on average, at slaughter. The polymorphisms were determined for the molecular markers CAPN316, CAPN530, CAPN4751, CAPN4753, and UOGACAST1. Analyses of meat tenderness at 7, 14, and 21 days of maturation were performed in samples of longissimus thoracis obtained between the 12th and 13th rib and sheared using a Warner Bratzler Shear Force. Significant effects were observed for meat tenderness at days 7, 14, and 21 of maturation for the marker CAPN4751, at day 21 for the marker CAPN4753, and at days 14 and 21 for the marker UOGCAST1. For genotypic combinations of markers, the results were significant for the combination CAPN4751/UOGCAST1 in the three maturation periods and CAPN4753/UOGCAST1 at days 14 and 21 of maturation.
authors.affiliation: Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brasil minosec@gmail.com.
authors.forename: M E
authors.initials: ME
authors.lastname: Carvalho
authors.affiliation: Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brasil.
authors.forename: J P
authors.initials: JP
authors.lastname: Eler
authors.affiliation: Embrapa Gado de Corte, Campo Grande, MS, Brasil.
authors.forename: M N
authors.initials: MN
authors.lastname: Bonin
authors.affiliation: Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Patos de Minas, MG, Brasil.
authors.forename: F M
authors.initials: FM
authors.lastname: Rezende
authors.affiliation: Department of Animal Sciences, Auburn University, Auburn, AL, USA.
authors.forename: F H
authors.initials: FH
authors.lastname: Biase
authors.affiliation: Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brasil.
authors.forename: F V
authors.initials: FV
authors.lastname: Meirelles
authors.affiliation: Embrapa Sudeste, São Carlos, SP, Brasil.
authors.forename: L C A
authors.initials: LC
authors.lastname: Regitano
authors.affiliation: Departamento de Ciência Animal, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brasil.
authors.forename: L L
authors.initials: LL
authors.lastname: Coutinho
authors.affiliation: Departamento de Nutrição e Produção Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Pirassununga, SP, Brasil.
authors.forename: J C C
authors.initials: JC
authors.lastname: Balieiro
authors.affiliation: Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brasil.
authors.forename: J B S
authors.initials: JB
authors.lastname: Ferraz
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002154:Calpain / Q000235:genetics*; D002417:Cattle; D005787:Gene Frequency; D005819:Genetic Markers; D005838:Genotype; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D000069466:Red Meat / Q000032:analysis
chemical_list: D002135:Calcium-Binding Proteins; D005819:Genetic Markers; C033668:calpastatin; D002154:Calpain