title: Lignocellulosic butanol production from Napier grass using semi-simultaneous saccharification fermentation.
abstract: Napier grass is a potential feedstock for biofuel production because of its strong adaptability and wide availability. Compositional analysis has been done on Napier grass which was collected from a local area of Taiwan. By comparing acid- and alkali-pretreatment, it was found that the alkali-pretreatment process is favorable for Napier grass. An overall glucose yield of 0.82g/g-glucosetotalcan be obtained with the combination of alkali-pretreatment (2.5wt% NaOH, 8wt% sample loading, 121°C, and a reaction time of 40min) and enzymatic hydrolysis (40FPU/g-substrate). Semi-simultaneous saccharification fermentation (sSSF) was carried out, where enzymatic hydrolysis and ABE fermentation were operated in the same batch. It was found that after 24-h hydrolysis, followed by 96-h fermentation, the butanol and acetone concentrations reached 9.45 and 4.85g/L, respectively. The butanol yield reached 0.22g/g-sugarglucose+xylose. Finally, the efficiency of butanol production from Napier grass was calculated at 31%.
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, Taichung, Taiwan.
authors.forename: Chi-Ruei
authors.initials: CR
authors.lastname: He
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, Taichung, Taiwan.
authors.forename: Yu-Yuan
authors.initials: YY
authors.lastname: Kuo
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, Taichung, Taiwan. Electronic address: syli@dragon.nchu.edu.tw.
authors.forename: Si-Yu
authors.initials: SY
authors.lastname: Li
mesh_terms: D000096:Acetone / Q000378:metabolism; D056804:Biofuels; D018533:Biomass; D000440:Butanols / Q000378:metabolism*; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002480:Cellulase / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000032:analysis; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D031782:Pennisetum / Q000378:metabolism*; D012972:Sodium Hydroxide / Q000494:pharmacology; D012997:Solvents / Q000494:pharmacology; D013464:Sulfuric Acids / Q000494:pharmacology; D013624:Taiwan; D014994:Xylose / Q000032:analysis
chemical_list: D056804:Biofuels; D000440:Butanols; D012997:Solvents; D013464:Sulfuric Acids; C036909:lignocellulose; D000096:Acetone; D000431:Ethanol; D012972:Sodium Hydroxide; D008031:Lignin; D014994:Xylose; D002480:Cellulase; D005947:Glucose; C033158:sulfuric acid
keywords: Alkali-pretreatment; Butanol fermentation; Enzymatic hydrolysis; Napier grass; Pennisetum purpureum; Semi-simultaneous saccharification fermentation (sSSF)