title: Genomic Analysis of a New Serovar ofLeptospira weiliiSerogroup Manhao.
abstract: Leptospirosis, caused by pathogenicLeptospiraspp., is recognized as an important emerging zoonotic disease throughout the world. In this study, multiple approaches were used to characterize the recently discovered serovar Heyan strain L231. This strain can infect guinea pigs and belonged to the pathogenic speciesL. weilii. Genome sequencing analysis revealed the draft genome of 4.2 M bp with a G+C content of 40.67% for strain L231, and a total of 4,794 ORFs were identified. The strain L231 genome was found to have a larger LPS biosynthesis locus than that of strainsL. interrogansserovar Lai andL. borgpeterseniiserovar Hardjobovis. Phylogenomic reconstructions showed that the evolutionary position ofL. weiliiserovar Heyan was different from that of other serovars from serogroup Manhao. These findings may lead us to a better understanding ofLeptospirapathogenesis and evolution.
authors.affiliation: Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control Beijing, China.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Key Laboratory of Reproduction Regulation of NPFPC, Shanghai Institute of Planned Parenthood Research, IRD, Fudan UniversityShanghai, China; Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Zhangjiang Hi-Tech ParkShanghai, China.
authors.forename: Huajun
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control Beijing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Zhangjiang Hi-Tech Park Shanghai, China.
authors.forename: Yuezhu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control Beijing, China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control Beijing, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control Beijing, China.
authors.forename: Shenghui
authors.initials: S
authors.lastname: Cui
authors.affiliation: Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control Beijing, China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Xin
authors.affiliation: Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control Beijing, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ye
authors.affiliation: Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca NY, USA.
authors.forename: Yung-Fu
authors.initials: YF
authors.lastname: Chang
authors.affiliation: Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control Beijing, China.
authors.forename: Junzhi
authors.initials: J
authors.lastname: Wang
keywords: Leptospirosis; genome; new serovar; pathogenesis; serogroup Manhao