title: Metabolic engineering of Escherichia coli to produce gamma-aminobutyric acid using xylose.
abstract: Biomass-derived xylose is an economically interesting substrate for the sustainable microbial production of value-added compounds. Escherichia coli could barely use xylose to directly produce gamma-aminobutyric acid. In this study, E. coli strains that could directly produce gamma-aminobutyric acid were developed through the deletion of eight genes sucA, puuE, gabT, gabP, xylA, xylB, waaC, and waaF, and the overexpression of two E. coli genes gadB and gdhA, as well as five Caulobacter crescent genes CcxylA, CcxylB, CcxylC, CcxylD, and CcxylX. Both E. coli strains W3110 and JM109 could directly produce gamma-aminobutyric acid from xylose after either overexpression of the seven genes or deletion of the eight genes. Overexpression of the seven genes of in the multiple deletion mutants further increased gamma-aminobutyric acid production. Among the 28 recombinant E. coli strains constructed in this study, the highest gamma-aminobutyric acid was produced by JWZ08/pWZt7-g3/pWZt7-xyl. JWZ08/pWZt7-g3/pWZt7-xyl could produce 3.95 g/L gamma-aminobutyric acid in flask cultivation, using xylose as the sole carbon source.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 xLihu Avenue, Wuxi, 214122, China.|School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
authors.forename: Anqi
authors.initials: A
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 xLihu Avenue, Wuxi, 214122, China.|Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, 214122, China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 xLihu Avenue, Wuxi, 214122, China. xwang@jiangnan.edu.cn.|School of Biotechnology, Jiangnan University, Wuxi, 214122, China. xwang@jiangnan.edu.cn.|Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, 214122, China. xwang@jiangnan.edu.cn.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Wang
mesh_terms: D016934:Caulobacter / Q000201:enzymology / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D017353:Gene Deletion; D015870:Gene Expression; D060847:Metabolic Engineering / Q000379:methods*; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics; D014994:Xylose / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D005680:gamma-Aminobutyric Acid; D014994:Xylose
keywords: Escherichia coli; GABA; Gamma-aminobutyric acid; Glu; L-glutamic acid; Xylose