title: Influence of Oxidative Stress on Biocontrol Activity ofCryptococcus laurentiiagainst Blue Mold on Peach Fruit.
abstract: The limitations of chemical fungicides for the control of postharvest diseases have recently become more apparent. The utilization of antagonistic microorganisms is a promising alternative to that of fungicides to control postharvest decay. In previous studies, the antagonistic yeastCryptococcus laurentiihas shown excellent effects of biocontrol and great potential for practical application. Adverse conditions, such as oxidative stress, limit the practical application of antagonistic yeast. In this study, we investigated the oxidative stress tolerance ofC. laurentiiand the associated mechanisms. The results indicated that exogenous oxidative stress has a significant effect on the viability and biocontrol efficiency ofC. laurentii. H2O2-induced oxidative stress led to the accumulation of reactive oxygen species. The results of flow cytometric analysis suggested that apoptosis is responsible for the reduced survival rate ofC. laurentiiunder oxidative stress. Using tests of antioxidant activity, we found thatC. laurentiicould employ enzymatic systems to resist exogenous oxidative stress. The addition of exogenous glutathione, a non-enzymatic antioxidant, to the media can significantly enhance oxidative tolerance and biocontrol efficiency ofC. laurentii.
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences Beijing, China.
authors.forename: Zhanquan
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of SciencesBeijing, China; College of Life Sciences, University of Chinese Academy of SciencesBeijing, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences Beijing, China.
authors.forename: Boqiang
authors.initials: B
authors.lastname: Li
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of SciencesBeijing, China; College of Life Sciences, University of Chinese Academy of SciencesBeijing, China.
authors.forename: Chang
authors.initials: C
authors.lastname: He
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of SciencesBeijing, China; College of Life Sciences, University of Chinese Academy of SciencesBeijing, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences Beijing, China.
authors.forename: Shiping
authors.initials: S
authors.lastname: Tian
keywords: Cryptococcus laurentii; antioxidant systems; apoptosis; biocontrol; glutathione; oxidative stress