title: Manipulating Cholesterol Status Within Cells.
abstract: Cellular cholesterol levels are intricately controlled to maintain homeostasis. Here, we describe ways in which cellular cholesterol status can be manipulated for the study of cholesterol homeostasis, including sterol starvation (by culturing cells in lipoprotein-deficient serum and pretreating/treating with the cholesterol-lowering drug, statin) and sterol enrichment (using cholesterol complexed to cyclodextrin, and low-density lipoprotein). We also describe how to prepare lipoprotein-deficient serum and complex cholesterol to cyclodextrin.
authors.affiliation: School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Biological Sciences Building D26, Sydney, NSW, 2052, Australia.
authors.forename: Winnie
authors.initials: W
authors.lastname: Luu
authors.affiliation: Faculty of Pharmacy, The University of Sydney, Sydney, NSW, 2006, Australia.
authors.forename: Ingrid C
authors.initials: IC
authors.lastname: Gelissen
authors.affiliation: School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Biological Sciences Building D26, Sydney, NSW, 2052, Australia. aj.brown@unsw.edu.au.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Brown
mesh_terms: D000818:Animals; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism*; D003505:Cyclodextrins / Q000737:chemistry / Q000494:pharmacology*; D006706:Homeostasis / Q000187:drug effects*; D006801:Humans; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D003505:Cyclodextrins; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D008077:Lipoproteins, LDL; D002784:Cholesterol
keywords: Chinese hamster ovary cells; Cholesterol; LDL; Lipoprotein-deficient serum; Statins