title: Transplantation of autologous chondrocytesex-vivoexpanded using Thermoreversible Gelation Polymer in a rabbit model of articular cartilage defect.
abstract: Graft failure due to de-differentiation of the chondrocytes during in vitro culture and after transplantation is a major hurdle in Autologous Chondrocyte Implantation (ACI). We, herein, report the transplantation of autologous chondrocytesex vivoexpanded using a Thermo-reversible Gelation Polymer (TGP) in a rabbit model. A full thickness chondral defect was created in one of the knee joints in each of the six rabbits of the study and autologous chondrocytesin vitroexpanded using TGP scaffold were transplanted after 10 weeks. H & E staining of the biopsy after 6 months revealed maintenance of articular cartilage phenotype.
authors.affiliation: Sri Ramachandra Arthroscopy & Sports Sciences Centre (SRASSC), Sri Ramachandra University, Chennai, India.
authors.forename: Sivaraman
authors.initials: S
authors.lastname: Arumugam
authors.affiliation: Sri Ramachandra Arthroscopy & Sports Sciences Centre (SRASSC), Sri Ramachandra University, Chennai, India.
authors.forename: Balasubramanyan
authors.initials: B
authors.lastname: Bhupesh Karthik
authors.affiliation: Sri Ramachandra Arthroscopy & Sports Sciences Centre (SRASSC), Sri Ramachandra University, Chennai, India.
authors.forename: Rajeswar
authors.initials: R
authors.lastname: Chinnuswami
authors.affiliation: Mebiol Inc., Hiratsuka, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Mori
authors.affiliation: Mebiol Inc., Hiratsuka, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoshioka
authors.affiliation: The Fujio-Eiji Academic Terrain (FEAT), Nichi-In Centre for Regenerative Medicine (NCRM), Chennai, India.
authors.forename: Rajappa
authors.initials: R
authors.lastname: Senthilkumar
authors.affiliation: The Fujio-Eiji Academic Terrain (FEAT), Nichi-In Centre for Regenerative Medicine (NCRM), Chennai, India.
authors.forename: Rajmohan
authors.initials: R
authors.lastname: Mathaiyan
authors.affiliation: The Fujio-Eiji Academic Terrain (FEAT), Nichi-In Centre for Regenerative Medicine (NCRM), Chennai, India.
authors.forename: Karthick
authors.initials: K
authors.lastname: Ramalingam
authors.affiliation: The Fujio-Eiji Academic Terrain (FEAT), Nichi-In Centre for Regenerative Medicine (NCRM), Chennai, India; Hope Foundation (Trust), Chennai, India.
authors.forename: Preethy
authors.initials: P
authors.lastname: Senthilkumar
authors.affiliation: The Mary-Yoshio Translational Hexagon (MYTH), Nichi-In Centre for Regenerative Medicine (NCRM), Chennai, India; II Department of Surgery, Yamanashi University- School of Medicine, Chuo, Japan.
authors.forename: Samuel J K
authors.initials: SJ
authors.lastname: Abraham
keywords: Articular cartilage; Autologous chondrocyte implantation (ACI); De-differentiation; Thermo-reversible gelation polymer (TGP); Tissue engineering