title: Identification and characterization of Orf virus 050 protein proteolysis.
abstract: The Orf virus 050 (ORFV050) gene is located in the core region of the ORFV genome. It is similar to Vaccinia virus (VV) Copenhagen L4R, and encodes the DNA-binding virion core protein VP8, which has structures similar to the VV P25K core protein and may undergo similar proteolytic processing during virus assembly. Three conserved Ala-Gly-X motifs at putative cleavage sites were identified in ORFV050. To investigate the proteolysis of ORFV050 and its participation in viral assembly, full-length and site-directed mutant ORFV050 recombinant proteins were constructed and expressed. Two distinct protein bands of 28.5 and 25 kDa were detected in the infected cells using anti-ORFV050 polyclonal antiserum. A potential cleavage site was identified at amino acids 30-32 of ORFV050. Mutation of AG/A to (R) in ORFV050 abolished the process of proteolysis. ORFV050 is a late gene synthesized during viral replication in the host cytoplasm. According to these results, we conclude that ORFV050 undergoes proteolysis and plays an important role in viral assembly.
authors.affiliation: Department of Laboratory Medicine, The Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, Guangdong, People's Republic of China.|Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, The Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, Guangdong, People's Republic of China.
authors.forename: Han
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, The Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, Guangdong, People's Republic of China.|Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, The Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, Guangdong, People's Republic of China.
authors.forename: Junyi
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of Laboratory Medicine, The Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, Guangdong, People's Republic of China.|Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, The Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, Guangdong, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Ding
authors.affiliation: Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou, 510515, Guangdong, People's Republic of China.|Department of Laboratory Medicine, Nongken Centre Hospital of Guangdong Province, Zhanjiang, 524002, Guangdong, People's Republic of China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, The Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, Guangdong, People's Republic of China.|Department of Laboratory Medicine, Kiang Wu Hospital, Macau, 999078, People's Republic of China.
authors.forename: Meiying
authors.initials: M
authors.lastname: Liao
authors.affiliation: Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, The Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, Guangdong, People's Republic of China.|Medical Research Center, The Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, Guangdong, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Shao
authors.affiliation: Department of Laboratory Medicine, The Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, Guangdong, People's Republic of China.|Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, The Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, Guangdong, People's Republic of China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Luo
authors.affiliation: Department of Laboratory Medicine, School of Stomatology and Medicine, Foshan University, 5 Hebin Road, Foshan, 528000, Guangdong, People's Republic of China. sluo815@gmail.com.
authors.forename: Shuhong
authors.initials: S
authors.lastname: Luo
authors.affiliation: Department of Laboratory Medicine, The Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, Guangdong, People's Republic of China. 1725012289@qq.com.|Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, The Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, Guangdong, People's Republic of China. 1725012289@qq.com.
authors.forename: Chaohui
authors.initials: C
authors.lastname: Duan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral; D002460:Cell Line; D003593:Cytoplasm / Q000821:virology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004151:Dipeptides; D004474:Ecthyma, Contagious / Q000821:virology; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral / Q000235:genetics*; D008970:Molecular Weight; D009154:Mutation; D009923:Orf virus / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000502:physiology; D059748:Proteolysis; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012293:Rifampin / Q000494:pharmacology; D016415:Sequence Alignment; D017421:Sequence Analysis; D012756:Sheep; D014616:Vaccinia virus / Q000235:genetics; D014758:Viral Core Proteins / Q000235:genetics / Q000502:physiology; D014764:Viral Proteins / Q000235:genetics* / Q000302:isolation & purification*; D015678:Viral Structural Proteins / Q000235:genetics / Q000378:metabolism; D014771:Virion / Q000378:metabolism; D019065:Virus Assembly / Q000502:physiology; D014779:Virus Replication
chemical_list: D000914:Antibodies, Viral; D004268:DNA-Binding Proteins; D004151:Dipeptides; D011993:Recombinant Fusion Proteins; D014758:Viral Core Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C108986:vaccinia virus protein VP8; C028872:alanylglycine; D012293:Rifampin
keywords: Cleavage site; ORFV050; Orf virus; Polyclonal antiserum; Proteolysis