title: Expression of microRNA-30c via lentivirus vector inhibits the proliferation and enhances the sensitivity of highly aggressive ccRCC Caki-1 cells to anticancer agents.
abstract: The clear cell renal cell carcinoma (ccRCC) is one of the most fatal urologic tumors, and the prognosis remains very poor for advanced or metastatic ccRCC. This study reveals the roles of microRNA (miR)-30c in regulating a highly aggressive ccRCC cell line proliferation by targeting MTA-1, which is a key mediator for human cancer metastasis. Results from quantitative real-time polymerase chain reaction showed that the expression of MTA-1, the target of miR-30c, was significantly higher in metastatic ccRCC specimens than in nonmetastatic ccRCC or nontumor specimens. Accordingly, endogenous miR-30c is at a much lower level in highly aggressive ccRCC Caki-1 cells than nontumor or ccRCC cell lines. Expression of miR-30c via lentivirus vector inhibits the proliferation, anchorage-independent growth, in vitro invasion or migration, or in vivo growth of Caki-1 cells by repressing MTA-1 protein expression. miR-30c also enhances the sensitivity of Caki-1 cells to anticancer agents, including sorafenib and paclitaxel. These data reveal the potential application of miR-30c and that its targeting gene, MTA-1, would be a potential target in metastatic ccRCC treatment.
authors.affiliation: Department of Laboratory Medicine, The first people's Hospital of Wujiang District, Suzhou.
authors.forename: Honglin
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Urinary Surgery, The First Affiliated Hospital of Harbin Medical University; Key Laboratory of Cardiovascular Medicine Research, Harbin Medical University, Ministry of Education, Harbin.
authors.forename: Erlin
authors.initials: E
authors.lastname: Song
authors.affiliation: Department of Laboratory Medicine, The first people's Hospital of Wujiang District, Suzhou.
authors.forename: Guorong
authors.initials: G
authors.lastname: Shen
authors.affiliation: Department of Laboratory Medicine, The first people's Hospital of Wujiang District, Suzhou.
authors.forename: Tonghua
authors.initials: T
authors.lastname: Zhu
authors.affiliation: Changshu Institution for Laboratory Medicine, Changshu.
authors.forename: Tingwang
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Department of Laboratory Medicine, The first people's Hospital of Wujiang District, Suzhou.
authors.forename: Hao
authors.initials: H
authors.lastname: Shen
authors.affiliation: Department of Laboratory Medicine, The first people's Hospital of Wujiang District, Suzhou.
authors.forename: Liping
authors.initials: L
authors.lastname: Niu
authors.affiliation: Department of Laboratory Medicine, The first people's Hospital of Wujiang District, Suzhou.
authors.forename: Biao
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Ultrasound Diagnosis, The first people's Hospital of Wujiang District, Suzhou, People's Republic of China.
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Changshu Institution for Laboratory Medicine, Changshu.
authors.forename: Jianping
authors.initials: J
authors.lastname: Qian
keywords: Caki-1; MTA-1; ccRCC; miR-30c; paclitaxel; sorafenib