title: MicroRNA-155 targets MAP3K10 and regulates osteosarcoma cell growth.
abstract: Osteosarcoma is the most common type of bone cancer prevalent in young adults. Recent studies suggested that aberrant microRNA expression plays an essential role in osteosarcoma pathogenesis. In this study, we found miR-155 up-regulation in different osteosarcoma cell lines U2OS, Saos-2 and MG-63. Through bioinformatic prediction and analysis, we identified its target MAP3K10 that involved in cell proliferation and apoptosis. This work demonstrates novel interaction between microRNA, intercellular MAPK signaling and apoptosis in osteosarcoma, which will provide targets for therapeutic development.
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of Zhengzhou University, No. 1 JianShe Road, Zhengzhou, Henan 450000, China.
authors.forename: Chuangjian
authors.initials: C
authors.lastname: Wang
authors.affiliation: Central sterile supply Department, The First Affiliated Hospital of Zhengzhou University, No. 1 JianShe Road, Zhengzhou, Henan 450000, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of Zhengzhou University, No. 1 JianShe Road, Zhengzhou, Henan 450000, China.
authors.forename: Chunlin
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of Zhengzhou University, No. 1 JianShe Road, Zhengzhou, Henan 450000, China.
authors.forename: Fuying
authors.initials: F
authors.lastname: Zhai
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of Zhengzhou University, No. 1 JianShe Road, Zhengzhou, Henan 450000, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of Zhengzhou University, No. 1 JianShe Road, Zhengzhou, Henan 450000, China. Electronic address: zdyfywcj@sina.com.
authors.forename: Zongqiang
authors.initials: Z
authors.lastname: Huang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D055785:Gene Knockdown Techniques; D064593:Heterografts; D006801:Humans; D020930:MAP Kinase Kinase Kinases / Q000096:biosynthesis*; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D012516:Osteosarcoma / Q000473:pathology*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D020930:MAP Kinase Kinase Kinases; C093290:MAP3K10 protein, human
keywords: Cell lines; MAP4K10; MicroRNA-155; Osteosarcoma