title: TIPE2 Inhibits the Expression of Asthma-Related Inflammatory Factors in Hyperstretched Bronchial Epithelial Cells Through the Wnt/β-Catenin Pathway.
abstract: Childhood asthma, an airway inflammatory disease, is a serious threat to the child's quality of life. Recently, TIPE2 expression was reported to be decreased in children with asthma. Therefore, additional studies focusing on TIPE2 might provide an approach for treating childhood asthma. In this study, we found that TIPE2 was poorly expressed in hyperstretched human bronchial epithelial cells (BEAS-2B). TIPE2 overexpression also significantly suppressed the stretch-induced secretion of asthma-related inflammatory factors (TNF-α, TSLP, MMP-9, and VEGF). In contrast, TIPE2 inhibition significantly promoted the secretion of TNF-α, TSLP, MMP-9, and VEGF. Furthermore, overexpression of TIPE2 remarkably inhibited the activation of Wnt/β-catenin in hyperstretched BEAS-2B cells, while siTIPE2 activated Wnt/β-catenin in hyperstretched BEAS-2B cells. Further analysis showed that the Wnt/β-catenin signal inhibitor Dkk-1 could further enhance the TIPE2-induced suppression of Wnt/β-catenin signaling, which also suppressed the siTIPE2-induced secretion of TNF-α, TSLP, MMP-9, and VEGF in hyperstretched BEAS-2B cells. Dkk-1 reversed the effects of siRNA-TIPE2 on Wnt/β-catenin signaling and inflammatory cytokines. In summary, we have exhibited that TIPE2 inhibited the expression of asthma-related inflammatory factors in hyperstretched BEAS-2B cells by suppressing the Wnt/β-catenin signaling pathway. TIPE2 may be involved in airway inflammation during asthma attack, and it may be used as a potential therapeutic target for bronchial epithelial inflammation in childhood asthma.
authors.affiliation: First Department of Respiratory Medicine, Xi'an Children's Hospital, Xi'an, China, 710003.
authors.forename: Xinrong
authors.initials: X
authors.lastname: Sun
authors.affiliation: First Neonatal Department, Xi'an Children's Hospital, Xi'an, China, 710003.
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
authors.affiliation: First Neonatal Department, Xi'an Children's Hospital, Xi'an, China, 710003. wenyanchild@163.com.
authors.forename: Wen
authors.initials: W
authors.lastname: Yan
mesh_terms: D001249:Asthma / Q000378:metabolism / Q000473:pathology*; D001696:Biomechanical Phenomena; D001980:Bronchi / Q000473:pathology*; D002460:Cell Line; D016207:Cytokines / Q000037:antagonists & inhibitors / Q000378:metabolism; D004847:Epithelial Cells / Q000473:pathology*; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D051153:Wnt Proteins / Q000378:metabolism; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000378:metabolism
chemical_list: C495270:CTNNB1 protein, human; D016207:Cytokines; D047908:Intracellular Signaling Peptides and Proteins; C552057:TNFAIP8L2 protein, human; D051153:Wnt Proteins; D051176:beta Catenin
keywords: TIPE2; Wnt/β-catenin; bronchial epithelial cells; childhood asthma; inflammation