title: Quantification of PSP toxins in toxic shellfish matrices using post-column oxidation liquid chromatography and pre-column oxidation liquid chromatography methods suggests post-column oxidation liquid chromatography as a good monitoring method of choice.
abstract: Different shellfish samples were analyzed by Pre- and Post-Column Oxidation Liquid Chromatography to compare the toxins profiles and get information about the degree of accomplishment of both methods. Comparison of the results obtained, the linear correlation coefficient (r2= 0.94) and the paired t test (two tails, α = 0.05), indicated that there were not significant differences between both sets of data. Nevertheless, important differences related to toxins profiles were found: it was remarkable the difference in results for both Gonyautoxins 1 and 4 and Decarbamoylgonyautoxins 2 and 3, depending on the method of choice, due to an overestimation in the Pre-Column method. It was necessary to modify the elution conditions in the Post-Column method to avoid the interference of matrix peaks at retention times closer to the retention times of the calibrants, mostly when working with oyster and scallop matrices, although it is a good method to use routinely.
authors.affiliation: Dpt. of Analytical Chemistry, Science Faculty, University of Santiago de Compostela, 27002 Lugo, Spain.
authors.forename: Verónica
authors.initials: V
authors.lastname: Rey
authors.affiliation: Dpt. of Analytical Chemistry, Science Faculty, University of Santiago de Compostela, 27002 Lugo, Spain. Electronic address: anamaria.botana@usc.es.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Botana
authors.affiliation: Dpt. of Pharmacology, Veterinary Faculty, University of Santiago de Compostela, 27002 Lugo, Spain.
authors.forename: Luis M
authors.initials: LM
authors.lastname: Botana
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D057230:Limit of Detection; D008387:Marine Toxins / Q000032:analysis*; D010125:Ostreidae; D012530:Saxitoxin / Q000031:analogs & derivatives / Q000032:analysis; D012758:Shellfish; D057096:Shellfish Poisoning
chemical_list: D008387:Marine Toxins; D012530:Saxitoxin; C042154:gonyautoxin I; C043405:gonyautoxin II
keywords: Interferences; Paralytic shellfish poisoning; Post-column oxidation method; Pre-column oxidation method; Shellfish matrices; Toxins