title: Triticum aestivum WRAB18 functions in plastids and confers abiotic stress tolerance when overexpressed in Escherichia coli and Nicotiania benthamiana.
abstract: WRAB18, an ABA-inducible protein belongs to the third family of late embryogenesis abundant (LEA) proteins which can be induced by different biotic or abiotic stresses. In the present study, WRAB18 was cloned from the Zhengyin 1 cultivar of Triticum aestivum and overexpressed in Escherichia coli to explore its effects on the growth of E. coli under different abiotic stresses. Results suggested the enhanced exhibition of tolerance of E. coli to these stresses. Meanwhile, the WRAB18-transgenic tobacco plants were obtained to analyze the stress-related enzymatic activities of ascorbate peroxidase (APX), peroxidase (POD) and superoxide dismutase (SOD), and to quantify the content of malonaldehyde (MDA) under osmotic stress, high salinity, and low and high temperature stress. The activities of APX, POD and SOD in the transgenic tobacco lines were higher while the content of MDA was lower than those of WT lines. Moreover, plastid localization of WRAB18 in Nicotiana benthamiana plasma cells were found fusing with GFP. In addition, purified WRAB18 protein protected LDH (Lactate dehydrogenase) enzyme activity in vitro from various stress conditions. In brief, WRAB18 protein shows protective action behaving as a "molecular shield" in both prokaryotic and eukaryotic cells under various abiotic stresses, not only during ABA stress.
authors.affiliation: College of Life Sciences/State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Life Sciences/State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling, China.
authors.forename: Linsheng
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Life Sciences/State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling, China.
authors.forename: Yane
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Life Sciences/State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling, China.
authors.forename: Zhenqing
authors.initials: Z
authors.lastname: Bai
authors.affiliation: College of Life Sciences/State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Life Sciences/State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling, China.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000222:Adaptation, Physiological / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D009997:Osmotic Pressure; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D018087:Plastids / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D054712:Salinity; D013312:Stress, Physiological; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D014908:Triticum / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
chemical_list: D010937:Plant Growth Regulators; D010940:Plant Proteins; D000040:Abscisic Acid