title: Normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity, in patients undergoing cataract surgery at Phramongkutklao Hospital.
abstract: OBJECTIVE
  This study aimed to evaluate the normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity.
  
  
  METHODS
  This was a prospective cross-sectional study.
  
  
  METHODS
  A prospective study was conducted on 120 patients who underwent cataract surgery at the Phramongkutklao Hospital from September 2014 to October 2014. Conjunctival and lid margin swabs were obtained from patients before they underwent cataract surgery. These swabs were used to inoculate blood agar and chocolate agar plates for culturing. After growth of the normal flora, the antibiotic sensitivity method using tobramycin, moxifloxacin, levofloxacin, and cefazolin was applied.
  
  
  METHODS
  Normal flora of conjunctiva and lid margin, along with its antibiotic sensitivity, from patients who underwent cataract surgery was assessed.
  
  
  RESULTS
  A total of 120 eyes were included in this study, and bacterial isolation rates were identified. Five bacteria from the lid margin were cultured, namely, coagulase-negative staphylococcus (58.33%),Streptococcusspp. (2.5%),Corynebacterium(1.67%),Micrococcusspp. (1.67%), andStaphylococcus aureus(0.83%). Two bacteria from the conjunctiva were cultured, namely, coagulase-negative staphylococcus (30%) andStreptococcusspp. (0.83%). Results of antibiotic sensitivity test showed that all isolated bacteria are sensitive to cefazolin 100%, tobramycin 98.67%, levofloxacin 100%, and moxifloxacin 100%.
  
  
  CONCLUSIONS
  Coagulase-negative staphylococci are the most common bacteria isolated from conjunctiva and lid margin.
authors.affiliation: Department of Ophthalmology, Phramongkutklao Hospital, Bangkok, Thailand.
authors.forename: Ravee
authors.initials: R
authors.lastname: Ratnumnoi
authors.affiliation: Department of Ophthalmology, Phramongkutklao Hospital, Bangkok, Thailand.
authors.forename: Narumon
authors.initials: N
authors.lastname: Keorochana
authors.affiliation: Department of Ophthalmology, Phramongkutklao Hospital, Bangkok, Thailand.
authors.forename: Chavalit
authors.initials: C
authors.lastname: Sontisombat
keywords: antibiotics; cataract surgery; conjunctival flora; lid margin flora; sensitivity