title: Chinese herbal medicineYouguiPill reduces exogenous glucocorticoid-induced apoptosis in anterior pituitary cells.
abstract: Long-term glucocorticoid use may result in sustained suppression of one or more secreted components from the hypothalamo-pituitary-adrenal axis, and often results in apoptosis.YouguiPill (YGP), a 10-component traditional Chinese herbal medicine, has been shown to be clinically effective for glucocorticoid-induced suppression of the hypothalamo-pituitary-adrenal axis. However, the pharmacological and molecular mechanisms remain unclear. We hypothesized that YGP would exert an anti-apoptosis effect on dexamethasone-treated anterior pituitary cells.In vivoexperiments showed that YGP significantly reduced the number of apoptotic cells, down-regulated mRNA expression of cytochrome c, caspase-3, and caspase-9, and up-regulated mRNA expression of Bcl-2. These findings suggest that YGP reduced glucocorticoid-induced apoptosis in rat anterior pituitary cells by regulating the mitochondria-mediated apoptosis pathway.
authors.affiliation: Department of Dermatology, Second Hospital of Jilin University, Changchun, Jinlin Province, China.
authors.forename: Yong-Zhi
authors.identifier: 0000-0002-0696-2142
authors.initials: YZ
authors.lastname: Ji
authors.affiliation: Department of Dermatology, First Hospital of China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Long
authors.identifier: 0000-0002-4754-4967
authors.initials: L
authors.lastname: Geng
authors.affiliation: Department of Dermatology, First Hospital of China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Hong-Bo
authors.initials: HB
authors.lastname: Zhou
authors.affiliation: Department of Dermatology, Mount Sinai Medical Center, New York, USA.
authors.forename: Hua-Chen
authors.initials: HC
authors.lastname: Wei
authors.affiliation: Department of Dermatology, First Hospital of China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Hong-Duo
authors.initials: HD
authors.lastname: Chen
keywords: Bcl-2; Yougui Pill; apoptosis; caspase-3; caspase-9; cytochrome c; glucocorticoid; mitochondrial membrane potential; nerve regeneration; neural regeneration