title: [Genetic polymorphisms of KIR2DS4 gene among ethnic Hans from southern China].
abstract: OBJECTIVE
  To study genetic polymorphisms of the KIR2DS4 gene among ethnic Hans from southern China.
  
  
  METHODS
  Genomic DNA was isolated from 306 unrelated individuals and amplified with KIR2DS4-specific PCR primers. KIR2DS4-positive samples were genotyped for the entire coding sequence by sequencing-based typing (SBT). Assignment of allelic genotypes was accomplished by using Assign 3.5 software. For samples with inconclusive SBT results, RT-PCR products covering the entire coding sequence of the KIR2DS4 gene were subjected to cloning and haplotype sequencing.
  
  
  RESULTS
  Among all tested samples, 297 were demonstrated to have carried the KIR2DS4 framework gene. For KIR2DS4-positive samples subjected to SBT for the entire coding sequences, no background was observed with the obtained sequences. Three of the seven identified alleles were of novel types, which were officially named by the KIR subcommittee of the World Health Organization Nomenclature Committee for Factors of HLA System. The observed frequencies for the 7 alleles were KIR2DS4*00101 (78.8%), *003 (10.5%), *004 (16.0%), *010 (23.2%), *017 (0.3%), *00105 (0.3%) and *018 (0.7%), respectively. Allele KIR2DS4*007 was not found. The overall frequency for normal cell-surface expression KIR2DS4 alleles including 2DS4*00101, *017 and *00105 was 79.4%, and that for non cell-surface expression alleles including 2DS4*003, *004, *010 and *018 was 50.4%. The ratio between the two was 1.6:1.
  
  
  CONCLUSIONS
  The present study has elucidated the allelic diversity of KIR2DS4 among ethnic Hans from southern China, which may provide valuable data for transplantation as well as studies on KIR-associated disease and evolution.
authors.affiliation: Shenzhen Blood Center, Shenzhen, Guangdong 518035, China. zhihui_deng@aliyun.com.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Zhen
authors.forename: Guobin
authors.initials: G
authors.lastname: Zhang
authors.forename: Qiong
authors.initials: Q
authors.lastname: Yu
authors.forename: Liumei
authors.initials: L
authors.lastname: He
authors.forename: Yunping
authors.initials: Y
authors.lastname: Xu
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zou
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Deng
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics; D001483:Base Sequence; D002681:China; D005787:Gene Frequency; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods*; D006239:Haplotypes; D006801:Humans; D011110:Polymorphism, Genetic; D054340:Receptors, KIR / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: C473953:KIR2DS4 protein, human; D054340:Receptors, KIR