title: Molecular cloning and characterization of an estrogen receptor gene in the marine polychaete Perinereis aibuhitensis.
abstract: Estrogen receptors (ERs) are the primary mediators of estrogen signaling, and play crucial roles in the reproduction and development of vertebrates. The full-length cDNA of Perinereis aibuhitensis estrogen receptor (paER) was cloned and characterized for the first time. The positions of the cysteine residues and the residues around them, which constitute two zinc finger motifs and a P-box, are conserved in both vertebrates and invertebrates. A phylogenetic analysis revealed that paER is an orthologue of ER in the polychaete Platynereis dumerilii. A tissue distribution analysis of paER mRNA showed that it is expressed in various tissues, including the body wall, head, esophageal gland, esophagus, stomach, and most strongly in the intestines. Its expression was also measured in P. aibuhitensis after exposure to 17β-estradiol (E2) for 48h. The paER mRNA levels in the body wall were measured after 6, 12, 24, and 48h in E2-exposed and control animals. However, no significant differences in paER expression were observed between them at any time point. This report describes the first molecular characterization of full-length paER and its tissue-specific expression in P. aibuhitensis.
authors.affiliation: Key Laboratory of Aquaculture and Ecology of Coastal Pool of Jiangsu Province, Department of Ocean Technology, Yancheng Institute of Technology, Yancheng, Jiangsu 224003, China.
authors.forename: Linlan
authors.initials: L
authors.lastname: Lv
authors.affiliation: Key Laboratory of Aquaculture and Ecology of Coastal Pool of Jiangsu Province, Department of Ocean Technology, Yancheng Institute of Technology, Yancheng, Jiangsu 224003, China; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China. Electronic address: ycitdong@126.com.
authors.forename: Xuexing
authors.initials: X
authors.lastname: Dong
authors.affiliation: Key Laboratory of Aquaculture and Ecology of Coastal Pool of Jiangsu Province, Department of Ocean Technology, Yancheng Institute of Technology, Yancheng, Jiangsu 224003, China.
authors.forename: Fu
authors.initials: F
authors.lastname: Lv
authors.affiliation: Key Laboratory of Aquaculture and Ecology of Coastal Pool of Jiangsu Province, Department of Ocean Technology, Yancheng Institute of Technology, Yancheng, Jiangsu 224003, China.
authors.forename: Weihong
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Aquaculture and Ecology of Coastal Pool of Jiangsu Province, Department of Ocean Technology, Yancheng Institute of Technology, Yancheng, Jiangsu 224003, China.
authors.forename: Yebin
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Key Laboratory of Aquaculture and Ecology of Coastal Pool of Jiangsu Province, Department of Ocean Technology, Yancheng Institute of Technology, Yancheng, Jiangsu 224003, China.
authors.forename: Wenping
authors.initials: W
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D059001:Aquatic Organisms; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism / Q000494:pharmacology; D015870:Gene Expression; D009928:Organ Specificity; D010802:Phylogeny; D011077:Polychaeta / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D011960:Receptors, Estrogen / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D016335:Zinc Fingers
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D011994:Recombinant Proteins; D004958:Estradiol
keywords: 17β-Estradiol; Estrogen receptor; Perinereis aibuhitensis; Tissue distribution; cDNA cloning