title: Lipidomics, en route to accurate quantitation.
abstract: Accurate quantitation is prerequisite for the sustainable development of lipidomics via enabling its applications in various biological and biomedical settings. In this review, the technical considerations and limitations of existent lipidomics technologies, particularly in terms of accurate quantitation; as well as the potential sources of errors along a typical lipidomic workflow that could ultimately give rise to quantitative inaccuracies will be addressed. Furthermore, the pressing need to exercise stricter definitions of terms and protocol standardization pertaining to quantitative lipidomics will be critically discussed, as quantitative accuracy may substantially impact upon the persevering development of lipidomics in the long run. This article is part of a Special Issue entitled: BBALIP_Lipidomics Opinion Articles edited by Sepp Kohlwein.
authors.affiliation: State Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
authors.forename: Sin Man
authors.initials: SM
authors.lastname: Lam
authors.affiliation: State Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
authors.forename: He
authors.initials: H
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China; Lipidall Technologies Company Limited, Changzhou 213000, Jiangsu, People's Republic of China. Electronic address: ghshui@genetics.ac.cn.
authors.forename: Guanghou
authors.initials: G
authors.lastname: Shui
mesh_terms: D000068598:Data Accuracy; D005069:Evaluation Studies as Topic; D006801:Humans; D050356:Lipid Metabolism / Q000502:physiology*; D008055:Lipids / Q000737:chemistry*; D055432:Metabolomics / Q000379:methods; D012015:Reference Standards; D013672:Technology / Q000379:methods; D057188:Workflow
chemical_list: D008055:Lipids
keywords: Accurate quantitation; Internal standards; Lipid extraction; Lipidomics