title: Preparation of a monolithic cation-exchange material with hydrophilic external layers by two-step reversible addition-fragmentation chain transfer polymerization.
abstract: In recent years, the efficient analysis of biological samples has become more important due to the advances of life science and pharmaceutical research and practice. Because biological sample pretreatment is the bottleneck for fast process, material development for efficient sample process in the high-performance liquid chromatography analysis is highly desirable. In this research, a cation-exchange restricted access monolithic column was synthesized by a reversible addition-fragmentation chain transfer polymerization method. Utilizing the controlled/living property of the reversible addition-fragmentation chain transfer method, a monolithic column of cross-linked poly(sulfopropyl methacrylate) was prepared first and then linear poly(glycerol mono-methacrylate) was immobilized covalently on the surface of the polymer. The monolithic material has both functionalities of cation-exchange and protein exclusion. Protein recovery of 94.6% was obtained after grafting of poly(glycerol mono-methacrylate) while the cation-exchange property of the column is still retained. In the study, the relation between the synthetic conditions and properties of the materials was studied. The synthesis conditions including the porogen, monomer concentration, and ratio of monomers/initiator/reversible addition-fragmentation chain transfer agent were optimized. The study provided a method for the preparation of restricted access monolithic columns: a bifunctional material by reversible addition-fragmentation chain transfer polymerization method.
authors.affiliation: Research Centre for Analytical Sciences, Tianjin Key Laboratory of Biosensing and Molecular Recognition, College of Chemistry, Nankai University, Tianjin, China.
authors.forename: Shen
authors.initials: S
authors.lastname: Lin
authors.affiliation: Research Centre for Analytical Sciences, Tianjin Key Laboratory of Biosensing and Molecular Recognition, College of Chemistry, Nankai University, Tianjin, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Research Centre for Analytical Sciences, Tianjin Key Laboratory of Biosensing and Molecular Recognition, College of Chemistry, Nankai University, Tianjin, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.affiliation: Research Centre for Analytical Sciences, Tianjin Key Laboratory of Biosensing and Molecular Recognition, College of Chemistry, Nankai University, Tianjin, China.
authors.forename: Xiangchao
authors.initials: X
authors.lastname: Dong
mesh_terms: D002411:Cation Exchange Resins; D002412:Cations; D002851:Chromatography, High Pressure Liquid; D057927:Hydrophobic and Hydrophilic Interactions; D008689:Methacrylates; D058105:Polymerization; D011506:Proteins / Q000302:isolation & purification*
chemical_list: D002411:Cation Exchange Resins; D002412:Cations; D008689:Methacrylates; D011506:Proteins
keywords: cation-exchange columns; monoliths; polymerization; protein exclusion; restricted access materials