title: Fluorescence lifetime imaging with time-gated detection of hyaluronidase using a long lifetime azadioxatriangulenium (ADOTA) fluorophore.
abstract: A fluorescence lifetime imaging probe with a long lifetime was used in combination with time-gating for the detection of hyaluronidase using hyaluronic acid as the probe template. This probe was developed by heavily labeling hyaluronic acid with long lifetime azadioxatriangulenium fluorophores (ADOTA). We used this probe to image hyaluronidase produced by DU-145 prostate cancer cells.
authors.affiliation: Institute of Molecular Medicine, Center for Fluorescence Technologies and Nanomedicine, University of North Texas Health Science Center, Fort Worth, TX 76107, USA. Department of Food Science, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA.
authors.forename: Rahul
authors.initials: R
authors.lastname: Chib
authors.forename: Sebastian
authors.initials: S
authors.lastname: Requena
authors.forename: Mark
authors.initials: M
authors.lastname: Mummert
authors.forename: Yuri M
authors.initials: YM
authors.lastname: Strzhemechny
authors.forename: Ignacy
authors.initials: I
authors.lastname: Gryczynski
authors.forename: Julian
authors.initials: J
authors.lastname: Borejdo
authors.forename: Zygmunt
authors.initials: Z
authors.lastname: Gryczynski
authors.forename: Rafal
authors.initials: R
authors.lastname: Fudala
mesh_terms: D001372:Aza Compounds; D045744:Cell Line, Tumor; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes; D006801:Humans; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase / Q000378:metabolism*; D007476:Ionophores; D008297:Male; D008856:Microscopy, Fluorescence; D061848:Optical Imaging; D013050:Spectrometry, Fluorescence
chemical_list: D001372:Aza Compounds; D005456:Fluorescent Dyes; D007476:Ionophores; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase