title: Repeatable deep-tissue activation of persistent luminescent nanoparticles by soft X-ray for high sensitivity long-term in vivo bioimaging.
abstract: Persistent luminescent nanoparticles (PLNPs) have emerged as important nanomaterials for biological imaging as a result of complete avoidance of tissue auto-fluorescence. However, the imaging sensitivity and long-term in vivo imaging are still limited due to the persistent luminescence that is rapidly decayed in vivo after an ex vivo excitation. To address this limitation, in vivo activation of PLNPs is highly desired. Herein, we present a new strategy for the activation of PLNPs (SrAl2O4:Eu2+) by using soft X-ray excitation. Importantly, as the soft X-ray light source possesses the advantage of deep tissue penetration, the PLNPs can be reactivated in vivo through living tissue using soft X-ray excitation. Furthermore, X-ray/persistent luminescence dual-modal imaging can be achieved to empower this strategy with high sensitivity. Our results suggest that this new strategy of in vivo energy charging in PLNPs would bring new insights for deep tissue and long-term bioimaging in living animals, and provide new perspectives for persistent luminescence bioimaging and therapeutic applications.
authors.affiliation: The Key Lab of Analysis and Detection Technology for Food Safety of the MOE and Fujian Province, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou 350002, China. hhyang@fzu.edu.cn lijuan@fzu.edu.cn.
authors.forename: Liang
authors.initials: L
authors.lastname: Song
authors.affiliation: The Key Lab of Analysis and Detection Technology for Food Safety of the MOE and Fujian Province, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou 350002, China. hhyang@fzu.edu.cn lijuan@fzu.edu.cn.
authors.forename: Xia-Hui
authors.initials: XH
authors.lastname: Lin
authors.affiliation: The Key Lab of Analysis and Detection Technology for Food Safety of the MOE and Fujian Province, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou 350002, China. hhyang@fzu.edu.cn lijuan@fzu.edu.cn.
authors.forename: Xiao-Rong
authors.initials: XR
authors.lastname: Song
authors.affiliation: The Key Lab of Analysis and Detection Technology for Food Safety of the MOE and Fujian Province, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou 350002, China. hhyang@fzu.edu.cn lijuan@fzu.edu.cn.
authors.forename: Shan
authors.initials: S
authors.lastname: Chen
authors.affiliation: The Key Lab of Analysis and Detection Technology for Food Safety of the MOE and Fujian Province, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou 350002, China. hhyang@fzu.edu.cn lijuan@fzu.edu.cn.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Chen
authors.affiliation: The Key Lab of Analysis and Detection Technology for Food Safety of the MOE and Fujian Province, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou 350002, China. hhyang@fzu.edu.cn lijuan@fzu.edu.cn.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: The Key Lab of Analysis and Detection Technology for Food Safety of the MOE and Fujian Province, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou 350002, China. hhyang@fzu.edu.cn lijuan@fzu.edu.cn.
authors.forename: Huang-Hao
authors.identifier: http://orcid.org/0000-0001-5894-0909
authors.initials: HH
authors.lastname: Yang
mesh_terms: D000818:Animals; D056945:Hep G2 Cells; D006801:Humans; D049449:Luminescence; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053758:Nanoparticles; D009374:Neoplasms, Experimental / Q000000981:diagnostic imaging*; D011859:Radiography; D014965:X-Rays