title: Erythrocyte-Platelet Hybrid Membrane Coating for Enhanced Nanoparticle Functionalization.
abstract: Cell-membrane-coated nanoparticles have recently been studied extensively for their biological compatibility, retention of cellular properties, and adaptability to a variety of therapeutic and imaging applications. This class of nanoparticles, which has been fabricated with a variety of cell membrane coatings, including those derived from red blood cells (RBCs), platelets, white blood cells, cancer cells, and bacteria, exhibit properties that are characteristic of the source cell. In this study, a new type of biological coating is created by fusing membrane material from two different cells, providing a facile method for further enhancing nanoparticle functionality. As a proof of concept, the development of dual-membrane-coated nanoparticles from the fused RBC membrane and platelet membrane is demonstrated. The resulting particles, termed RBC-platelet hybrid membrane-coated nanoparticles ([RBC-P]NPs), are thoroughly characterized, and it is shown that they carry properties of both source cells. Further, the [RBC-P]NP platform exhibits long circulation and suitability for further in vivo exploration. The reported strategy opens the door for the creation of biocompatible, custom-tailored biomimetic nanoparticles with varying hybrid functionalities, which may be used to overcome the limitations of current nanoparticle-based therapeutic and imaging platforms.
authors.affiliation: Department of NanoEngineering and Moores Cancer Center, University of California, San Diego, La Jolla, CA, 92093, USA.
authors.forename: Diana
authors.initials: D
authors.lastname: Dehaini
authors.affiliation: Department of NanoEngineering and Moores Cancer Center, University of California, San Diego, La Jolla, CA, 92093, USA.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wei
authors.affiliation: Department of NanoEngineering and Moores Cancer Center, University of California, San Diego, La Jolla, CA, 92093, USA.
authors.forename: Ronnie H
authors.initials: RH
authors.lastname: Fang
authors.affiliation: Department of NanoEngineering and Moores Cancer Center, University of California, San Diego, La Jolla, CA, 92093, USA.
authors.forename: Sarah
authors.initials: S
authors.lastname: Masson
authors.affiliation: Department of NanoEngineering and Moores Cancer Center, University of California, San Diego, La Jolla, CA, 92093, USA.
authors.forename: Pavimol
authors.initials: P
authors.lastname: Angsantikul
authors.affiliation: Department of NanoEngineering and Moores Cancer Center, University of California, San Diego, La Jolla, CA, 92093, USA.
authors.forename: Brian T
authors.initials: BT
authors.lastname: Luk
authors.affiliation: Department of NanoEngineering and Moores Cancer Center, University of California, San Diego, La Jolla, CA, 92093, USA.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of NanoEngineering and Moores Cancer Center, University of California, San Diego, La Jolla, CA, 92093, USA.
authors.forename: Man
authors.initials: M
authors.lastname: Ying
authors.affiliation: Department of NanoEngineering and Moores Cancer Center, University of California, San Diego, La Jolla, CA, 92093, USA.
authors.forename: Yao
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of NanoEngineering and Moores Cancer Center, University of California, San Diego, La Jolla, CA, 92093, USA.
authors.forename: Ashley V
authors.initials: AV
authors.lastname: Kroll
authors.affiliation: Department of NanoEngineering and Moores Cancer Center, University of California, San Diego, La Jolla, CA, 92093, USA.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Gao
authors.affiliation: Department of NanoEngineering and Moores Cancer Center, University of California, San Diego, La Jolla, CA, 92093, USA.
authors.forename: Liangfang
authors.initials: L
authors.lastname: Zhang
mesh_terms: D032701:Biomimetics; D001792:Blood Platelets; D004910:Erythrocyte Membrane; D004912:Erythrocytes; D053758:Nanoparticles
keywords: biomimetic nanoparticles; long circulation; membrane fusion; nanomedicine; targeted delivery