title: Highly selective optical and reversible dual-path chemosensor for cyanide detection and its application in live cells imaging.
abstract: A new biocompatible fluorescent receptor 1 was synthesized by conjugating diaminomaleonitrile (DMN) with benzothiazole unit, and characterized by single X-ray. In DMF/water (1:1, v/v), the receptor 1 showed a selectivity turn-on fluorescence at 517nm in the presence of CN-. Receptor 1 showed a detection limit down to 0.16µM without any interference from other tested anions. The reversibility and reusability of 1 for the detection of CN-ion was also tested for five cycles indicating the probe 1 could be used in a reversible manner. Importantly, the receptor 1 showed excellent cells viability and was successfully applied for the detection of CN-in live mouse fibroblast cells L929 cells.
authors.affiliation: Department of Chemistry, Indian Institute of Technology Bombay, Powai, Mumbai 400076 India.
authors.forename: Karunesh
authors.initials: K
authors.lastname: Keshav
authors.affiliation: School of Chemical Science, North Maharashtra University, Jalgaon 425001, India.
authors.forename: Pritam
authors.initials: P
authors.lastname: Torawane
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors.forename: Mukesh
authors.initials: M
authors.lastname: Kumar Kumawat
authors.affiliation: School of Chemical Science, North Maharashtra University, Jalgaon 425001, India.
authors.forename: Kundan
authors.initials: K
authors.lastname: Tayade
authors.affiliation: Department of Applied Chemistry, SV National Institute of Technology, Surat 395007 India.
authors.forename: Suban K
authors.initials: SK
authors.lastname: Sahoo
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors.forename: Rohit
authors.initials: R
authors.lastname: Srivastava
authors.affiliation: School of Chemical Science, North Maharashtra University, Jalgaon 425001, India. Electronic address: kuwaras@gmail.com.
authors.forename: Anil
authors.initials: A
authors.lastname: Kuwar
mesh_terms: D000818:Animals; D052160:Benzothiazoles / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D002460:Cell Line; D003486:Cyanides / Q000032:analysis*; D005347:Fibroblasts / Q000166:cytology; D005456:Fluorescent Dyes / Q000737:chemistry*; D057230:Limit of Detection; D051379:Mice; D008958:Models, Molecular; D009570:Nitriles / Q000737:chemistry*; D061848:Optical Imaging / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D052160:Benzothiazoles; D003486:Cyanides; D005456:Fluorescent Dyes; D009570:Nitriles; C022103:diaminomaleonitrile; C005465:benzothiazole
keywords: Biocompatibility; Cellular imaging; Cyanide; DFT; Fluorescent turn-on sensor