title: NLRP3 p.Q705K and CARD8 p.C10X single nucleotide polymorphisms are not associated with susceptibility to rheumatoid arthritis: a meta-analysis.
abstract: OBJECTIVE
  Genetic factors have a substantial contribution to the pathogenesis of rheumatoid arthritis (RA). Single nucleotide polymorphisms of NLRP3 p.Q705K and CARD8 p.C10X are two gene mutations that have been linked to many diseases. Here we carried out a meta-analysis to identify their association with susceptibility to RA.
  
  
  METHODS
  Relevant studies were identified from databases, including PubMed, Cochrane Library, EMBase, Elsevier Science Direct, Web of Science, SpringerLink, and so on. Data extracted from selected studies were analyzed using the Version 12.0 STATA software. Pooled odds ratios (ORs) were calculated as the effect sizes for comparisons.
  
  
  RESULTS
  In total, six case-control studies from five articles that contained 2705 RA patients and 2711 healthy controls were included. (i) The NLRP3 p.Q705K polymorphism in allelic model (OR = 0.908), genotypic models (OR1 = 0.786; OR2 = 0.916; OR3 = 0.729), dominant (OR = 0.909) and recessive models (OR = 0.778) were not associated with the risk of RA (all P > 0.05). (ii) The CARD8 p.C10X polymorphism in allelic model (OR = 0.995,), genotypic models (OR1 = 0.997; OR2 = 1.052; OR3 = 0.950), dominant (OR = 1.033) and recessive models (OR = 0.963) were not associated with the risk of RA (all P > 0.05). (iii) When compared with combined genotype CARD8/NLRP3 AA/CC, none of the other combined genotypes had significant pooled ORs (all P > 0.05). (iv) Individuals carrying at least one variant allele at each of the two loci showed no more susceptibility to RA than those carrying only wild-type alleles at both the NLRP3 p.Q705K and CARD8 p.C10X loci (OR = 1.056, P > 0.05).
  
  
  CONCLUSIONS
  NLRP3 p.Q705K and CARD8 p.C10X polymorphisms were not associated with the susceptibility to RA, separately or in combined forms.
authors.affiliation: Department of Immunology, Shandong University School of Medicine, Ji'nan, China.|Department of Rheumatology, Jinan Central Hospital affiliated to Shandong University, Ji'nan, China.
authors.forename: Zhaowen
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Rheumatology, Provincial Hospital affiliated to Shandong University, Ji'nan, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Cao
authors.affiliation: Department of Rheumatology, Provincial Hospital affiliated to Shandong University, Ji'nan, China.
authors.forename: Qingrui
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Department of Rheumatology, Provincial Hospital affiliated to Shandong University, Ji'nan, China.
authors.forename: Yuanchao
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Immunology, Shandong University School of Medicine, Ji'nan, China.
authors.forename: Lihui
authors.initials: L
authors.lastname: Han
mesh_terms: D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics*; D053468:CARD Signaling Adaptor Proteins / Q000235:genetics*; D016009:Chi-Square Distribution; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics*; D016017:Odds Ratio; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: D053468:CARD Signaling Adaptor Proteins; C455623:CARD8 protein, human; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C453881:NLRP3 protein, human; D009363:Neoplasm Proteins
keywords: CARD8; NLRP3; meta-analysis; rheumatoid arthritis; single nucleotide polymorphism