title: Omp16-based vaccine encapsulated by alginate-chitosan microspheres provides significant protection against Haemophilus parasuis in mice.
abstract: Haemophilus parasuis (H. parasuis) is the etiological agent of swine Glässer's disease, which leads to significant economic loss in swine industry over the world. Subunit vaccine based on outer membrane protein is one of the promising choices to protect pigs against H. parasuis infection despite low immunity efficiency. In this paper, outer membrane protein 16 (Omp16) of H. parasuis encapsulated by alginate-chitosan microspheres as antigen carriers was explored for the first time in a mouse model. Our results showed that the microspheres with Omp16 induced significant higher H. parasuis-specific antibodies, and higher titers of IL-2, IL-4, and IFN-γ than those by Omp16-FIA in treated mice (p<0.05). Moreover, H. parasuis load in the tissues from liver, spleen, and lung of mice immunized with microspheres containing Omp16 was significantly decreased (p<0.05) than that in the same counterpart tissues of control groups. In addition, 80% mice treated with Omp16 and 70% mice with Omp16-FIA were survived after challenged with H. parasuis virulent strain LY02 (serovar 5). Therefore, Omp16-based microsphere vaccine induces both humoral and cellular immune responses and provides promising protection against H. parasuis infection in mice.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong, PR China; College of Life Science, Longyan University, Longyan, Fujian, PR China; Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan, Fujian, PR China.
authors.forename: Xintian
authors.initials: X
authors.lastname: Zheng
authors.affiliation: College of Life Science, Longyan University, Longyan, Fujian, PR China; Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan, Fujian, PR China. Electronic address: lyyxy1988@126.com.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Yang
authors.affiliation: College of Life Science, Longyan University, Longyan, Fujian, PR China; Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan, Fujian, PR China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Life Science, Longyan University, Longyan, Fujian, PR China; Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan, Fujian, PR China.
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Qiu
authors.affiliation: College of Life Science, Longyan University, Longyan, Fujian, PR China; Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan, Fujian, PR China.
authors.forename: Ailing
authors.initials: A
authors.lastname: Dai
authors.affiliation: College of Life Science, Longyan University, Longyan, Fujian, PR China; Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan, Fujian, PR China.
authors.forename: Qichun
authors.initials: Q
authors.lastname: Huang
authors.affiliation: College of Life Science, Longyan University, Longyan, Fujian, PR China; Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan, Fujian, PR China.
authors.forename: Cuiqin
authors.initials: C
authors.lastname: Huang
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong, PR China. Electronic address: xfguo@scau.edu.cn.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Guo
mesh_terms: D000464:Alginates / Q000008:administration & dosage; D000825:Animal Structures / Q000382:microbiology; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000276:immunology*; D058491:Bacterial Load; D001425:Bacterial Outer Membrane Proteins / Q000008:administration & dosage / Q000276:immunology*; D048271:Chitosan / Q000008:administration & dosage; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004337:Drug Carriers / Q000008:administration & dosage*; D020723:Glucuronic Acid / Q000008:administration & dosage; D006192:Haemophilus Infections / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D018073:Haemophilus Vaccines / Q000008:administration & dosage / Q000276:immunology*; D044137:Haemophilus parasuis / Q000276:immunology*; D006603:Hexuronic Acids / Q000008:administration & dosage; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008807:Mice, Inbred BALB C; D008863:Microspheres; D016019:Survival Analysis; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*; D016896:Treatment Outcome; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000464:Alginates; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D016207:Cytokines; D004337:Drug Carriers; D018073:Haemophilus Vaccines; D006603:Hexuronic Acids; D022223:Vaccines, Subunit; D020723:Glucuronic Acid; D048271:Chitosan
keywords: Alginate; Chitosan; Haemophilus parasuis; Microspheres; Omp16