title: Plasma prefractionation methods for proteomic analysis and perspectives in clinical applications.
abstract: Plasma is a rich source of biomarkers with clinical relevance. However, the wide dynamic range of protein concentration hinders the detection of low abundance proteins. Plasma prefractionation methods serve as indispensable tools to reduce plasma complexity, allowing the opportunity to explore tissue-derived proteins which leak into the circulation. This review summarizes common approaches in plasma prefractionation methods for proteomic analysis and then discusses some considerations in plasma prefractionation for clinical applications, reviewing some examples of its use in clinical situations.
authors.affiliation: Pediatric Translational Research Unit, Department of Pediatrics, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Salaya, Thailand.
authors.forename: Somchai
authors.initials: S
authors.lastname: Chutipongtanate
authors.affiliation: Department of Tropical Pediatrics, Faculty of Tropical Medicine, Mahidol University, Salaya, Thailand.
authors.forename: Supawat
authors.initials: S
authors.lastname: Chatchen
authors.affiliation: Laboratory of Biochemistry, Chulabhorn Research Institute, Krung Thep Maha Nakhon, Thailand.|Applied Biological Sciences Program, Chulabhorn Graduate Institute, Thailand.
authors.forename: Jisnuson
authors.initials: J
authors.lastname: Svasti
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification* / Q000378:metabolism*; D005591:Chemical Fractionation / Q000379:methods*; D006801:Humans; D040901:Proteomics / Q000379:methods*
chemical_list: D001798:Blood Proteins
keywords: Biomarker; Depletion; Enrichment; Fractionation; Plasma