title: Pitfalls of invasive blood pressure monitoring using the caudal ventral artery in rats.
abstract: During rodent experiments, the caudal ventral artery (CVA) is useful for blood pressure (BP) measurement. However, CVA measurements may not reflect the true BP. This study was performed to verify the site-specific accuracy of invasive arterial BP monitoring during surgery in rats. Invasive arterial BP was simultaneously measured in rats via the CVA and the common carotid artery (CCA). The BP values were analysed while the rats were subjected to cooling of the head or tail. Additionally, the rats underwent digital subtraction angiography and histological examination of these arteries. The pressure difference was more significant in the tail cooling group than in the head cooling group. Digital subtraction angiography revealed that angiospasms occurred more frequently in the CVA than in the CCA upon cooling. This phenomenon was supported by histological analysis, which showed that the tunica media area was significantly larger in the CVA than in the CCA. CVA pressure is susceptible to environmental changes and may not accurately reflect the true BP without a strictly controlled laboratory environment. Therefore, understanding the pitfalls of this method is necessary to avoid cooling of the tail during BP measurement.
authors.affiliation: Division of Regenerative Medicine, Research Center for Medical Sciences, the Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan.|Division of Vascular Surgery, Department of Surgery, the Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ohta
authors.affiliation: Division of Vascular Surgery, Department of Surgery, the Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Ohki
authors.affiliation: Division of Vascular Surgery, Department of Surgery, the Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kanaoka
authors.affiliation: Laboratory Animal Facilities, Research Center for Medical Sciences, the Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Koizumi
authors.affiliation: Division of Regenerative Medicine, Research Center for Medical Sciences, the Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan.
authors.forename: Hirotaka J
authors.initials: HJ
authors.lastname: Okano
mesh_terms: D000818:Animals; D001794:Blood Pressure; D001795:Blood Pressure Determination / Q000379:methods* / Q000592:standards; D002339:Carotid Arteries / Q000502:physiology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013623:Tail / Q000098:blood supply*