title: Kisspeptin-10 inhibits proliferation and regulates lipolysis and lipogenesis processes in 3T3-L1 cells and isolated rat adipocytes.
abstract: BACKGROUND
  Kisspeptin, which is encoded by the KISS1 gene and acts via GPR54, plays a role in the regulation of reproductive functions. Expression of KISS1 and GRPR54 has been found in peripheral tissues, including adipose tissue, and was shown to be influenced by metabolic status.
  
  
  OBJECTIVE
  We hypothesized that kisspeptin could be involved in regulation of lipid metabolism in the mouse 3T3-L1 cell line and in isolated rat adipocytes.
  
  
  METHODS
  First, we characterized expression profiles of KISS1 and GPR54 mRNA and proteins in adipose cells isolated from male rats. Secondly, we studied the effects of kisspeptin-10 on cell proliferation and survival in 3T3-L1 cells. Thirdly, we assessed the rapid action of kisspeptin-10 on lipid metabolism and glucose uptake using 3T3-L1 cells and rat primary adipocytes. Finally, we examined the effects of kisspeptin-10 on the secretion of leptin and adiponectin in rat adipocytes.
  
  
  RESULTS
  We have found that: (1) KISS1 and GPR54 were expressed in mouse 3T3-L1 cells and isolated rat adipocytes; (2) kisspeptin-10: (i) inhibited cell proliferation, viability and adipogenesis in 3T3-L1 and decreased expression of PPAR-γ and CEBPβ-genes, which are involved in the differentiation processes and adipogenesis; (ii) increased lipolysis in 3T3-L1 cells and rat adipocytes by enhancing expression of periliphin and hormone-sensitive lipase; (iii) modulated glucose uptake and lipogenesis; (iv) stimulated leptin and decreased adiponectin secretion from rat adipocytes.
  
  
  CONCLUSIONS
  Kisspeptin-10 could play a role in the regulation of lipid metabolism in mouse 3T3-L1 cells and rat adipocytes.
authors.affiliation: Department of Animal Physiology and Biochemistry, Poznan University of Life Sciences, Wołynska 33, 60-625, Poznan, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Pruszyńska-Oszmałek
authors.affiliation: Department of Animal Physiology and Biochemistry, Poznan University of Life Sciences, Wołynska 33, 60-625, Poznan, Poland.
authors.forename: Paweł A
authors.initials: PA
authors.lastname: Kołodziejski
authors.affiliation: Department of Animal Physiology and Biochemistry, Poznan University of Life Sciences, Wołynska 33, 60-625, Poznan, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Sassek
authors.affiliation: Laboratory of Neurobiology, Institute of Zoology, Poznan University of Life Sciences, Wojska Polskiego 71C, 60-625, Poznan, Poland. joanna.sliwowska@gmail.com.
authors.forename: Joanna H
authors.initials: JH
authors.lastname: Sliwowska
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D052242:Adiponectin / Q000378:metabolism; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D059648:Kisspeptins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D020738:Leptin / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects*; D050155:Lipogenesis / Q000187:drug effects*; D008066:Lipolysis / Q000187:drug effects*; D051379:Mice; D051381:Rats; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D000074502:Receptors, Kisspeptin-1
chemical_list: D052242:Adiponectin; C106628:KISS1 protein, human; C491406:Kiss1 protein, rat; C492247:Kiss1r protein, rat; D059648:Kisspeptins; D020738:Leptin; D043562:Receptors, G-Protein-Coupled; D000074502:Receptors, Kisspeptin-1
keywords: 3T3-L1 cells; Adipocytes; GPR54; Kisspeptin