title: Aerobic anoxygenic phototrophs in gold mine tailings in Nopiming Provincial Park, Manitoba, Canada.
abstract: A sampling trip to Central Gold Mine, Nopiming Provincial Park, Canada, was taken in September 2011. Abundance, distribution, and physiology of aerobic anoxygenic phototrophs (AAP) from 4 locations were studied. Enumeration revealed 14.6% of culturable microbes were AAP. Five strains (NM4.16, NM4.18, C4, C9, C11) were chosen for analysis. All grow best on complex media without vitamin requirements and with an optimal pH 7.0-8.0, with strain C4 preferring pH 6.0. Strain NM4.18 tolerates the highest pH 11.0. Optimal temperature for all is 28 °C (range of 2-37 °C except NM4.16, which survives 45 °C). Strains C9, C11, and NM4.18 grew in 1.0%, 2.0%, and 5.0% NaCl, respectively, while NM4.16 and C4 grew only without NaCl. Isolates were all highly resistant to toxic metal(oid) oxides: tellurite (1500 μg/mL, all), tellurate (1500 μg/mL, C11), selenite (5000 μg/mL, C9, C11, and NM4.18), selenate (1000 μg/mL, C9 and C11), and orthometavanadate and metavanadate (5000 μg/mL, C11 and NM4.18). They could reduce tellurite to the less toxic elemental tellurium. Full 16S rRNA gene sequencing revealed all strains are Alphaproteobacteria, with C4 and NM4.16 closely related to Porphyrobacter colymbi (99.4% and 99.7% sequence similarity, respectively), C9 to Brevundimonas variabilis (99.1%), C11 to Brevundimonas bacteroides (98.6%), and NM4.18 to Erythromonas ursincola (98.5%).
authors.affiliation: a Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Hughes
authors.affiliation: a Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
authors.forename: Breanne
authors.initials: B
authors.lastname: Head
authors.affiliation: a Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
authors.forename: Chris
authors.initials: C
authors.lastname: Maltman
authors.affiliation: b Department of Biological Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
authors.forename: Michele
authors.initials: M
authors.lastname: Piercey-Normore
authors.affiliation: a Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Yurkov
mesh_terms: D020561:Alphaproteobacteria / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D002170:Canada; D004269:DNA, Bacterial / Q000235:genetics; D006046:Gold; D008350:Manitoba; D008906:Mining; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012965:Sodium Chloride / Q000494:pharmacology; D013691:Tellurium / Q000494:pharmacology; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D006046:Gold; C026660:tellurous acid; D013691:Tellurium
keywords: Nopiming Provincial Park; Parc provincial Nopiming; aerobic anoxygenic phototroph; gold mine tailings; phototrophe aérobie anoxygénique; résidus de la mine gold; tellurite