title: Specific membrane capacitance, cytoplasm conductivity and instantaneous Young's modulus of single tumour cells.
abstract: As label-free biomarkers, biophysical properties of cells are widely used for cell type classification. However, intrinsic biophysical markers, e.g., specific membrane capacitance (Cspecific membrane), cytoplasm conductivity (σconductivity) and instantaneous Young's modulus (Einstantaneous) measured for hundreds of single cells were not yet reported. In this study, single cells in suspension (adherent cells treated with trypsin) were aspirated through a microfluidic constriction channel at 25 °C, and the entry processes and impedance profiles were recorded and translated to Cspecific membrane, σconductivityand Einstantaneous. Cspecific membrane, σconductivityand Einstantaneousof five cell types were quantified as 2.10±0.38 μF cm-2, 0.91±0.15 S m-1and 5.52±0.95 kPa for H460 cells (ncell=437); 2.52±0.54 μF cm-2, 0.83±0.12 S m-1and 5.54±1.04 kPa for H446 cells (ncell=410); 2.45±0.57 μF cm-2, 0.99±0.18 S m-1and 5.16±1.68 kPa for A549 cells (ncell=442); 1.86±0.31 μF cm-2, 1.07±0.18 S m-1and 3.86±0.81 kPa for 95D cells (ncell=415); 2.03±0.35 μF cm-2, 0.99±0.16 S m-1and 3.49±0.70 kPa for 95C cells (ncell=290). The database of Cspecific membrane, σconductivityand Einstantaneousmay serve as a reference for future studies of cellular biophysical properties.
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, PR China.|School of Electronic, Electrical and Communication Engineering, University of Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, PR China.|R&D Center for Healthcare Electronics, Institute of Microelectronics, Chinese Academy of Sciences, Beijing 100029, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, PR China.|School of Electronic, Electrical and Communication Engineering, University of Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Deyong
authors.initials: D
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, PR China.|School of Electronic, Electrical and Communication Engineering, University of Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Beiyuan
authors.initials: B
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, PR China.|School of Electronic, Electrical and Communication Engineering, University of Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Yulan
authors.initials: Y
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, PR China.|School of Electronic, Electrical and Communication Engineering, University of Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Lianhong
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Mechanical Engineering, University of Colorado, Boulder, Colorado 80309, USA.
authors.forename: Rong
authors.initials: R
authors.lastname: Long
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, PR China.|School of Electronic, Electrical and Communication Engineering, University of Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Junbo
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, PR China.|School of Electronic, Electrical and Communication Engineering, University of Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
mesh_terms: D002462:Cell Membrane; D003593:Cytoplasm; D006801:Humans; D009369:Neoplasms / Q000473:pathology / Q000503:physiopathology; D059010:Single-Cell Analysis