title: Molecular Cloning, Bioinformatic Analysis, and Expression ofBombyx moriLebocin 5 Gene Related toBeauveria bassianaInfection.
abstract: A full-length cDNA of lebocin 5 (BmLeb5) was first cloned from silkworm,Bombyx mori, by rapid amplification of cDNA ends. The BmLeb5 gene is 808 bp in length and the open reading frame encodes a 179-amino acid hydroxyproline-rich peptide. Bioinformatic analysis results showed that BmLeb5 owns an O-glycosylation site and four RXXR motifs as other lebocins. Sequence similarity and phylogenic analysis results indicated that lebocins form a multiple gene family in silkworm as cecropins. Quantitative real-time PCR analysis revealed that BmLeb5 was highest expressed in the fat body. In the silkworm larvae infected byBeauveria bassiana, the expression level of BmLeb5 was upregulated in the fat body and hemolymph which are the most important immune tissues in silkworm. The recombinant protein of BmLeb5 was for the first time successfully expressed with prokaryotic expression system and purified. There are no reports so far that the expression of lebocins could be induced by entomopathogenic fungus. Our study suggested that BmLeb5 might play an important role in the immune response of silkworm to defendB. bassianainfection. The results also provided helpful information for further studying the lebocin family functioned in antifungal immune response in the silkworm.
authors.affiliation: School of Biotechnology and Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, China; Nursing School, Zhenjiang College, Zhenjiang 212003, China.
authors.forename: Dingding
authors.initials: D
authors.lastname: Lü
authors.affiliation: School of Biotechnology and Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, China.
authors.forename: Chengxiang
authors.initials: C
authors.lastname: Hou
authors.affiliation: School of Biotechnology and Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, China.
authors.forename: Guangxing
authors.initials: G
authors.lastname: Qin
authors.affiliation: School of Biotechnology and Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Gao
authors.affiliation: School of Biotechnology and Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Chen
authors.affiliation: School of Biotechnology and Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, China.
authors.forename: Xijie
authors.identifier: 0000-0002-7184-6914
authors.initials: X
authors.lastname: Guo
mesh_terms: D000818:Animals; D052982:Beauveria / Q000276:immunology*; D012831:Bombyx / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D003001:Cloning, Molecular; D019295:Computational Biology; D005786:Gene Expression Regulation / Q000276:immunology*; D019476:Insect Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology
chemical_list: D019476:Insect Proteins; C095582:lebocin protein, Bombyx mori