title: Validation study of a 15-plex rapid STR amplification system for human identification.
abstract: Conventional PCR amplification requires approximately 3h to complete and thus does not meet the requirements of rapid DNA analysis. The purpose of this study was to validate a rapid 15-plex PCR system that can amplify 14 autosomal short tandem repeat (STR) loci (i.e., D6S1043, D21S11, D7S820, CFS1PO, D2S1338, D3S1358, D13S317, D8S1179, D16S539, Penta E, D5S818, vWA, D18S51, and FGA) and Amelogenin. This system was validated by sensitivity, species specificity, inhibitor tests, sizing accuracy, stutter calculation, concordance tests, DNA mixture, and case sample tests according to the Scientific Working Group for DNA Analysis Methods (SWGDAM) guidelines and Chinese criteria. We found that the rapid 15-plex PCR system could shorten the amplification time to 37min and proved that it provides an alternative method for conventional PCR in human identification detection.
authors.affiliation: Key Laboratory of Forensic Genetics, Beijing Engineering Research Center of Crime Scene Evidence Examination, Institute of Forensic Science, Beijing, 100038, China; Technology Department of Chaoyang Sub-Bureau, Beijing Public Security Bureau, Beijing, 100102, China.
authors.forename: Junping
authors.initials: J
authors.lastname: Han
authors.affiliation: Key Laboratory of Forensic Genetics, Beijing Engineering Research Center of Crime Scene Evidence Examination, Institute of Forensic Science, Beijing, 100038, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.affiliation: Key Laboratory of Forensic Genetics, Beijing Engineering Research Center of Crime Scene Evidence Examination, Institute of Forensic Science, Beijing, 100038, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Forensic Genetics, Beijing Engineering Research Center of Crime Scene Evidence Examination, Institute of Forensic Science, Beijing, 100038, China.
authors.forename: Wenting
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Forensic Genetics, Beijing Engineering Research Center of Crime Scene Evidence Examination, Institute of Forensic Science, Beijing, 100038, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Forensic Genetics, Beijing Engineering Research Center of Crime Scene Evidence Examination, Institute of Forensic Science, Beijing, 100038, China. Electronic address: licaixia@tsinghua.edu.cn.
authors.forename: Caixia
authors.initials: C
authors.lastname: Li
mesh_terms: D053523:Amelogenin / Q000235:genetics; D000818:Animals; D016172:DNA Fingerprinting / Q000379:methods*; D053803:Forensic Genetics; D006801:Humans; D018895:Microsatellite Repeats; D060885:Multiplex Polymerase Chain Reaction / Q000295:instrumentation*; D013045:Species Specificity
chemical_list: D053523:Amelogenin
keywords: DNA typing; Forensic science; Rapid PCR