title: The Effects of Myo-Inositol and B and D Vitamin Supplementation in the db/+ Mouse Model of Gestational Diabetes Mellitus.
abstract: Gestational diabetes mellitus (GDM) is a growing concern, affecting an increasing number of pregnant women worldwide. By predisposing both the affected mothers and children to future disease, GDM contributes to an intergenerational cycle of obesity and diabetes. In order to stop this cycle, safe and effective treatments for GDM are required. This study sought to determine the treatment effects of dietary supplementation with myo-inositol (MI) and vitamins B2, B6, B12, and D in a mouse model of GDM (pregnant db/+ dams). In addition, the individual effects of vitamin B2 were examined. Suboptimal B2 increased body weight and fat deposition, decreased GLUT4 adipose tissue expression, and increased expression of inflammatory markers. MI supplementation reduced weight and fat deposition, and reduced expression of inflammatory markers in adipose tissue of mice on suboptimal B2. MI also significantly reduced the hyperleptinemia observed in db/+ mice, when combined with supplemented B2. MI was generally associated with adipose tissue markers of improved insulin sensitivity and glucose uptake, while the combination of vitamins B2, B6, B12, and D was associated with a reduction in adipose inflammatory marker expression. These results suggest that supplementation with MI and vitamin B2 could be beneficial for the treatment/prevention of GDM.
authors.affiliation: Liggins Institute, University of Auckland, Auckland 1021, New Zealand. jasmine.plows@auckland.ac.nz.
authors.forename: Jasmine F
authors.initials: JF
authors.lastname: Plows
authors.affiliation: Nestle Research Center, Lausanne 1000, Switzerland. florence.budin@rdls.nestle.com.
authors.forename: Florence
authors.initials: F
authors.lastname: Budin
authors.affiliation: Liggins Institute, University of Auckland, Auckland 1021, New Zealand. reban468@student.liu.se.
authors.forename: Rebecka A M
authors.initials: RA
authors.lastname: Andersson
authors.affiliation: Liggins Institute, University of Auckland, Auckland 1021, New Zealand. valeriejeanmills@gmail.com.
authors.forename: Valerie J
authors.initials: VJ
authors.lastname: Mills
authors.affiliation: Nestle Research Center, Lausanne 1000, Switzerland. catherine.mace@rdls.nestle.com.
authors.forename: Katherine
authors.initials: K
authors.lastname: Mace
authors.affiliation: University of Alberta, Edmonton, AB T6G 2R3, Canada. sandra.davidge@ualberta.ca.
authors.forename: Sandra T
authors.initials: ST
authors.lastname: Davidge
authors.affiliation: Liggins Institute, University of Auckland, Auckland 1021, New Zealand. m.vickers@auckland.ac.nz.
authors.forename: Mark H
authors.initials: MH
authors.lastname: Vickers
authors.affiliation: Liggins Institute, University of Auckland, Auckland 1021, New Zealand. philip.baker@leicester.ac.uk.|College of Medicine, Biological Sciences and Psychology, University of Leicester, Leicester LE1 7RH, UK. philip.baker@leicester.ac.uk.
authors.forename: Philip N
authors.initials: PN
authors.lastname: Baker
authors.affiliation: Nestle Research Center, Lausanne 1000, Switzerland. irma.silvazolezzi@rdls.nestle.com.
authors.forename: Irma
authors.initials: I
authors.lastname: Silva-Zolezzi
authors.affiliation: Liggins Institute, University of Auckland, Auckland 1021, New Zealand. j.stanley@auckland.ac.nz.
authors.forename: Joanna L
authors.initials: JL
authors.lastname: Stanley
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D016640:Diabetes, Gestational / Q000188:drug therapy*; D019587:Dietary Supplements; D004195:Disease Models, Animal; D005260:Female; D018836:Inflammation Mediators / Q000378:metabolism; D007294:Inositol / Q000008:administration & dosage*; D051379:Mice; D011247:Pregnancy; D012256:Riboflavin / Q000008:administration & dosage*; D014807:Vitamin D / Q000008:administration & dosage*; D014815:Vitamins / Q000008:administration & dosage*
chemical_list: D018836:Inflammation Mediators; D014815:Vitamins; D014807:Vitamin D; D007294:Inositol; D012256:Riboflavin
keywords: B12; B2; B6; D; LepR; Riboflavin; db/+; db/db; gestational diabetes; mice; myo-inositol