title: Celecoxib-mediated activation of endoplasmic reticulum stress induces de novo ceramide biosynthesis and apoptosis in hepatoma HepG2 cells mobilization.
abstract: Ceramides are the major sphingolipid metabolites involved in cell survival and apoptosis. When HepG2 hepatoma cells were treated with celecoxib, the expression of the genes in de novo sphingolipid biosynthesis and sphingomyelinase pathway was upregulated and cellular ceramide was elevated. In addition, celecoxib induced endoplasmic reticulum (ER) stress in a time-dependent manner. SPTLC2, a subunit of serine palmitoyltransferase, was overexpressed by adenovirus. Adenoviral overexpression of SPTLC2 (AdSPTLC2) decreased cell viability of HEK293 and HepG2 cells. In addition, AdSPTLC2 induced apoptosis via the caspase-dependent apoptotic pathway and elevated cellular ceramide, sphingoid bases, and dihydroceramide. However, overexpression of SPTLC2 did not induce ER stress. Collectively, celecoxib activates de novo sphingolipid biosynthesis and the combined effects of elevated ceramide and transcriptional activation of ER stress induce apoptosis. However, activation of de novo sphingolipid biosynthesis does not activate ER stress in hepatoma cells and is distinct from the celecoxib-mediated activation of ER stress. [BMB Reports 2017; 50(3): 144-149].
authors.affiliation: Department of Life Science, Gachon University, Seongnam 13120, Korea.
authors.forename: Hyo Jin
authors.initials: HJ
authors.lastname: Maeng
authors.affiliation: Department of Life Science, Gachon University, Seongnam 13120, Korea.
authors.forename: Jae-Hwi
authors.initials: JH
authors.lastname: Song
authors.affiliation: Department of Life Science, Gachon University, Seongnam 13120, Korea.
authors.forename: Goon-Tae
authors.initials: GT
authors.lastname: Kim
authors.affiliation: Department of Life Science, Gachon University, Seongnam 13120, Korea.
authors.forename: Yoo-Jeong
authors.initials: YJ
authors.lastname: Song
authors.affiliation: Department of Physiology, Konkuk University School of Medicine, Seoul 05030, Korea.
authors.forename: Kangpa
authors.initials: K
authors.lastname: Lee
authors.affiliation: Department of Life Science, Gachon University, Seongnam 13120, Korea.
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Department of Life Science, Gachon University, Seongnam 13120, Korea.
authors.forename: Tae-Sik
authors.initials: TS
authors.lastname: Park
mesh_terms: D017209:Apoptosis / Q000502:physiology; D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D000068579:Celecoxib / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival; D002518:Ceramides / Q000096:biosynthesis*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D059865:Endoplasmic Reticulum Stress / Q000502:physiology; D057809:HEK293 Cells; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism; D051102:Serine C-Palmitoyltransferase / Q000378:metabolism; D013107:Sphingolipids / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D002518:Ceramides; D013107:Sphingolipids; D051102:Serine C-Palmitoyltransferase; D000068579:Celecoxib