title: Cell lines derived from the squash bug, Anasa tristis (Coreidae: Hemiptera).
abstract: The squash bug, Anasa tristis, is a pest of cucurbits that exerts direct damage on crops and is a vector of plant pathogens. We established cell lines from this insect to serve as tools for basic biology, including virology and immunology, as well as applied studies, such as insecticide development programs. We initiated 15 cell cultures, using nine media or combinations of media. The media yielding the best results were a modification of Kimura's medium and a combination of two commercially available cell culture media (EX-CELL 420 and L15). We designated the two cell lines as BCIRL-AtE-CLG11 and BCIRL-AtE-CLG15. From the AtE-CLG15 line, we isolated two sub-lines, A and B. Of these, the most consistently replicating line was AtE-CLG15A. We determined the doubling time of this line (190 h) and its mean cell diameter (14.5 ± 0.7 μm). We characterized the AtE-CLG15A line using DAF-PCR. The BCIRL-AtE-CLG15A cell line is now available for researchers world-wide.
authors.affiliation: USDA, Agricultural Research Service, Biological Control of Insects Research Laboratory, 1503 S. Providence Road, Columbia, MO, 65203, USA. CINDY.GOODMAN@ARS.USDA.GOV.
authors.forename: Cynthia L
authors.initials: CL
authors.lastname: Goodman
authors.affiliation: USDA, Agricultural Research Service, Biological Control of Insects Research Laboratory, 1503 S. Providence Road, Columbia, MO, 65203, USA.
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Ringbauer
authors.affiliation: Plant Protection Institute, Hebei Academy of Agricultural and Forestry Sciences/IPM Center of Hebei Province/ Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, Baoding, 07100, China.
authors.forename: Yao-Fa
authors.initials: YF
authors.lastname: Li
authors.affiliation: USDA, Agricultural Research Service, Biological Control of Insects Research Laboratory, 1503 S. Providence Road, Columbia, MO, 65203, USA.
authors.forename: Tamra Reall
authors.initials: TR
authors.lastname: Lincoln
authors.affiliation: USDA, Agricultural Research Service, Biological Control of Insects Research Laboratory, 1503 S. Providence Road, Columbia, MO, 65203, USA.
authors.forename: David
authors.initials: D
authors.lastname: Stanley
mesh_terms: D000818:Animals; D002460:Cell Line / Q000166:cytology* / Q000187:drug effects; D028464:Cucurbita / Q000469:parasitology; D006430:Hemiptera / Q000166:cytology* / Q000187:drug effects* / Q000472:pathogenicity; D007306:Insecticides / Q000494:pharmacology*; D012621:Seasons
chemical_list: D007306:Insecticides
keywords: Anasa tristis; DAF-PCR; Hemiptera; Insect cell line; Squash bug