title: Phosphoproteomics: Protein Phosphorylation in Regulation of Seed Germination and Plant Growth.
abstract: Seed germination and plant growth are key stages in the plant life cycle and they are affected by abiotic stresses. Protein phosphorylation is commonly involved in key regulatory process, which mediates plant growth/development and stress responses. In plant, phosphoproteins play roles in stress responses and provide basic backbone in complex signaling networks. The mechanism of plant responses to abiotic stresses has been uncovered through study of protein phosphorylation. Recently, new methods for enrichment of phosphopeptides and phosphoproteins have been developed. Phosphoproteomic techniques are widely applied to studies of seed germination and plant growth. The signaling networks of phytohormones have been explored using the phosphoproteomic approach. In this review, the enrichment methods of phosphopeptides and phosphoproteins are summarized for phosphoproteomics. The roles of protein kinases and phosphatases in plant are described. The significant achievements of phosphoproteomics in the areas of seed germination and plant growth under abiotic stresses are reviewed. Applications of phosphoproteomics are valuable resources to uncover functional proteins regulating plant growth and stress responses.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan.|National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan.
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Yin
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan.|National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan.|National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan.
authors.forename: Setsuko
authors.initials: S
authors.lastname: Komatsu
mesh_terms: D018525:Germination; D010748:Phosphopeptides / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D063245:Plant Development / Q000502:physiology*; D010937:Plant Growth Regulators / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D010944:Plants / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism; D040901:Proteomics; D012639:Seeds / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D010748:Phosphopeptides; D010750:Phosphoproteins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011494:Protein Kinases
keywords: Phosphorylation; germination; growth; phytohormones.; plant; proteomics