title: Silver nanoparticles alter the permeability of sheep pleura and of sheep and human pleural mesothelial cell monolayers.
abstract: Nanoparticles have been implicated in the development of pleural effusions in exposed factory workers while in experimental animal studies it has been shown that they induce inflammation, fibrosis and carcinogenesis in the pleura. The scope of this study was to investigate the direct effects of silver nanoparticles exposure on the membrane permeability of sheep parietal pleura, of primary sheep pleural cell monolayers and on a human mesothelial cell line. Our findings suggest that acute (30min) exposure increases the pleural permeability ex vivo, while longer (24h) exposure in vivo leads to late decrease of the pleural cell monolayers permeability.
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Thessaly, BIOPOLIS, Larissa 41500, Greece.
authors.forename: Zoi V
authors.initials: ZV
authors.lastname: Arsenopoulou
authors.affiliation: Laboratory of Physiology, Faculty of Veterinary Medicine, Aristotle University, Thessaloniki, Greece.
authors.forename: Ioannis A
authors.initials: IA
authors.lastname: Taitzoglou
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Thessaly, BIOPOLIS, Larissa 41500, Greece.
authors.forename: Paschalis-Adam
authors.initials: PA
authors.lastname: Molyvdas
authors.affiliation: Department of Respiratory Medicine, Faculty of Medicine, University of Thessaly, BIOPOLIS, Larissa 41500, Greece.
authors.forename: Konstantinos I
authors.initials: KI
authors.lastname: Gourgoulianis
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Thessaly, BIOPOLIS, Larissa 41500, Greece.
authors.forename: Chrissi
authors.initials: C
authors.lastname: Hatzoglou
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Thessaly, BIOPOLIS, Larissa 41500, Greece. Electronic address: szarog@med.uth.gr.
authors.forename: Sotirios G
authors.initials: SG
authors.lastname: Zarogiannis
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D010539:Permeability; D010994:Pleura / Q000166:cytology*; D012756:Sheep; D012834:Silver / Q000633:toxicity*; D023382:Toxicity Tests, Acute
chemical_list: D012834:Silver
keywords: Electrophysiology; Mesothelium; Nanotoxicology; Pleura; Silver nanoparticles; Ussing system