title: Superficially porous particles with 1000Å pores for large biomolecule high performance liquid chromatography and polymer size exclusion chromatography.
abstract: To facilitate mass transport and column efficiency, solutes must have free access to particle pores to facilitate interactions with the stationary phase. To ensure this feature, particles should be used for HPLC separations which have pores sufficiently large to accommodate the solute without restricted diffusion. This paper describes the design and properties of superficially porous (also called Fused-Core®, core shell or porous shell) particles with very large (1000Å) pores specifically developed for separating very large biomolecules and polymers. Separations of DNA fragments, monoclonal antibodies, large proteins and large polystyrene standards are used to illustrate the utility of these particles for efficient, high-resolution applications.
authors.affiliation: Advanced Materials Technology, Inc., 3521 Silverside Rd., Ste. 1-K, Quillen Bldg, Wilmington, DE 19810, USA.
authors.forename: Brian M
authors.initials: BM
authors.lastname: Wagner
authors.affiliation: Advanced Materials Technology, Inc., 3521 Silverside Rd., Ste. 1-K, Quillen Bldg, Wilmington, DE 19810, USA. Electronic address: sschuster@advanced-materials-tech.com.
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Schuster
authors.affiliation: Advanced Materials Technology, Inc., 3521 Silverside Rd., Ste. 1-K, Quillen Bldg, Wilmington, DE 19810, USA.
authors.forename: Barry E
authors.initials: BE
authors.lastname: Boyes
authors.affiliation: Advanced Materials Technology, Inc., 3521 Silverside Rd., Ste. 1-K, Quillen Bldg, Wilmington, DE 19810, USA.
authors.forename: Taylor J
authors.initials: TJ
authors.lastname: Shields
authors.affiliation: Advanced Materials Technology, Inc., 3521 Silverside Rd., Ste. 1-K, Quillen Bldg, Wilmington, DE 19810, USA.
authors.forename: William L
authors.initials: WL
authors.lastname: Miles
authors.affiliation: Advanced Materials Technology, Inc., 3521 Silverside Rd., Ste. 1-K, Quillen Bldg, Wilmington, DE 19810, USA.
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Haynes
authors.affiliation: Advanced Materials Technology, Inc., 3521 Silverside Rd., Ste. 1-K, Quillen Bldg, Wilmington, DE 19810, USA.
authors.forename: Robert E
authors.initials: RE
authors.lastname: Moran
authors.affiliation: Advanced Materials Technology, Inc., 3521 Silverside Rd., Ste. 1-K, Quillen Bldg, Wilmington, DE 19810, USA.
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Kirkland
authors.affiliation: Theoretical Separation Science Laboratory, Kroungold Analytical, Inc., 1299 Butler Pike, Blue Bell, PA 19422, USA.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Schure
mesh_terms: D002850:Chromatography, Gel / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004247:DNA / Q000302:isolation & purification; D046911:Macromolecular Substances / Q000302:isolation & purification*; D010316:Particle Size; D011108:Polymers / Q000302:isolation & purification*; D016062:Porosity; D011506:Proteins / Q000302:isolation & purification
chemical_list: D046911:Macromolecular Substances; D011108:Polymers; D011506:Proteins; D004247:DNA
keywords: DNA fragments; HPLC; Monoclonal antibodies; Size exclusion chromatography (SEC); Superficially porous particles; Very large pores