title: Oxyresveratrol Supplementation to C57bl/6 Mice Fed with a High-Fat Diet Ameliorates Obesity-Associated Symptoms.
abstract: Oxyresveratrol has been proven effective in inhibiting adipogenesis in a 3T3-L1 cell model. We investigated the preventive effect of oxyresveratrol supplementation on obesity development in high-fat diet-fed mice. Male C57bl/6 mice were randomly subjected to control (5% fat by weight, LF), high-fat (30% fat by weight, HF), and high-fat supplemented with 0.25% and 0.5% oxyresveratrol (OXY1 and OXY2, respectively) diet groups for eight weeks. Oxyresveratrol supplementation effectively alleviated obesity-associated symptoms such as insulin resistance, hyperglycemia, and hepatic steatosis in high-fat diet-fed mice. Compared to the high-fat diet group, oxyresveratrol supplementation suppressed expression of glucose-6-phosphatase, sterol regulatory element-binding proteins 1, fatty acid synthase and CCAAT/Enhancer-binding proteins α, and elevated AMP-activated protein kinase (α2-catalytic subunit) level in liver, upregulated insulin-dependent glucose transporter type 4 level in adipose tissue, and increased expression of insulin receptor substrate 1, insulin-dependent glucose transporter type 4, AMP-activated protein kinase α, peroxisome proliferator-activated receptor γ coactivator-1α, and sirtuin 1 in muscle to regulate lipid and glucose homeostasis in these tissues. This study demonstrated that oxyresveratrol supplementation effectively ameliorated obesity-associated symptoms in high-fat diet-fed mice, presumably attributed to mediating critical regulators involved in lipid and glucose homeostasis in liver, visceral fat, and muscle.
authors.affiliation: School of Biological Sciences, The University of Hong Kong, Hong Kong, China. totanhy@gmail.com.
authors.forename: Hui Yuan
authors.initials: HY
authors.lastname: Tan
authors.affiliation: School of Biological Sciences, The University of Hong Kong, Hong Kong, China. mytsea@hku.hk.
authors.forename: Iris Mei Ying
authors.initials: IM
authors.lastname: Tse
authors.affiliation: School of Biological Sciences, The University of Hong Kong, Hong Kong, China. etsli@hku.hk.
authors.forename: Edmund Tsze Shing
authors.initials: ET
authors.lastname: Li
authors.affiliation: School of Biological Sciences, The University of Hong Kong, Hong Kong, China. mfwang@hku.hk.
authors.forename: Mingfu
authors.initials: M
authors.lastname: Wang
mesh_terms: D050156:Adipogenesis / Q000187:drug effects; D000818:Animals; D059305:Diet, High-Fat / Q000009:adverse effects*; D019587:Dietary Supplements; D005234:Fatty Liver / Q000517:prevention & control; D005947:Glucose / Q000378:metabolism; D006706:Homeostasis / Q000187:drug effects; D006943:Hyperglycemia / Q000517:prevention & control; D007333:Insulin Resistance; D050152:Intra-Abdominal Fat / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009132:Muscles / Q000378:metabolism; D009765:Obesity / Q000517:prevention & control*; D010936:Plant Extracts / Q000008:administration & dosage*; D013267:Stilbenes / Q000008:administration & dosage*
chemical_list: D010936:Plant Extracts; D013267:Stilbenes; C034912:puag-haad; D005947:Glucose
keywords: amelioration; glucose homeostasis; high-fat diet; lipid homeostasis; obesity; oxyresveratrol supplementation