title: Screening of faba bean (Vicia fabaL.) accessions to acidity and aluminium stresses.
abstract: BACKGROUND
  Faba bean is an important starch-based protein crop produced worldwide. Soil acidity and aluminium toxicity are major abiotic stresses affecting its production, so in regions where soil acidity is a problem, there is a gap between the potential and actual productivity of the crop. Hence, we set out to evaluate acidity and aluminium tolerance in a range of faba bean germplasm using solution culture and pot experiments.
  
  
  METHODS
  A set of 30 accessions was collected from regions where acidity and aluminium are or are not problems. The accessions were grown in solution culture and a subset of 10 was grown first in peat and later in perlite potting media. In solution culture, morphological parameters including taproot length, root regrowth and root tolerance index were measured, and in the pot experiments the key measurements were taproot length, plant biomass, chlorophyll concentration and stomatal conductance.
  
  
  RESULTS
  Responses to acidity and aluminium were apparently independent. Accessions Dosha and NC 58 were tolerant to both stress. Kassa and GLA 1103 were tolerant to acidity showing less than 3% reduction in taproot length. Aurora and Messay were tolerant to aluminium. Babylon was sensitive to both, with up to 40% reduction in taproot length from acidity and no detectable recovery from Al3+challenge.
  
  
  CONCLUSIONS
  The apparent independence of the responses to acidity and aluminium is in agreement with the previous research findings, suggesting that crop accessions separately adapt to H+and Al3+toxicity as a result of the difference in the nature of soil parent materials where the accession originated. Differences in rankings between experiments were minor and attributable to heterogeneity of seed materials and the specific responses of accessions to the rooting media. Use of perlite as a potting medium offers an ideal combination of throughput, inertness of support medium, access to leaves for detection of their stress responses, and harvest of clean roots for evaluation of their growth.
authors.affiliation: Department of Agricultural Sciences, Viikki Plant Science Centre, University of Helsinki , Helsinki , South Finland , Finland.
authors.forename: Kiflemariam Y
authors.initials: KY
authors.lastname: Belachew
authors.affiliation: Department of Food and Environmental Sciences, Viikki Plant Science Centre, University of Helsinki , Helsinki , Finland.
authors.forename: Frederick L
authors.initials: FL
authors.lastname: Stoddard
keywords: Abiotic stress; Acid; Aluminium; Faba bean; Hematoxylin; Low pH; Screening; Tolerance; Toxicity; Vicia