title: Optimization of laccase production from Marasmiellus palmivorus LA1 by Taguchi method of Design of experiments.
abstract: Fungal laccase has profound applications in different fields of biotechnology due to its broad specificity and high redox potential. Any successful application of the enzyme requires large scale production. As laccase production is highly dependent on medium components and cultural conditions, optimization of the same is essential for efficient product production.
  
  
  
  Production of laccase by fungal strain Marasmiellus palmivorus LA1 under solid state fermentation was optimized by the Taguchi design of experiments (DOE) methodology. An orthogonal array (L8) was designed using Qualitek-4 software to study the interactions and relative influence of the seven selected factors by one factor at a time approach. The optimum condition formulated was temperature (28 °C), pH (5), galactose (0.8%w/v), cupric sulphate (3 mM), inoculum concentration (number of mycelial agar pieces) (6Nos.) and substrate length (0.05 m). Overall yield increase of 17.6 fold was obtained after optimization. Statistical optimization leads to the elimination of an insignificant medium component ammonium dihydrogen phosphate from the process and contributes to a 1.06 fold increase in enzyme production. A final production of 667.4 ± 13 IU/mL laccase activity paves way for the application of this strain for industrial applications.
  
  
  
  Study optimized lignin degrading laccases from Marasmiellus palmivorus LA1. This laccases can thus be used for further applications in different scales of production after analyzing the properties of the enzyme. Study also confirmed the use of taguchi method for optimizations of product production.
authors.affiliation: Department of Biotechnology, Cochin University of Science and Technology, Cochin-22, Kerala, India.
authors.forename: Aiswarya
authors.initials: A
authors.lastname: Chenthamarakshan
authors.affiliation: Department of Biotechnology, Cochin University of Science and Technology, Cochin-22, Kerala, India.
authors.forename: Nayana
authors.initials: N
authors.lastname: Parambayil
authors.affiliation: Department of Biotechnology, Cochin University of Science and Technology, Cochin-22, Kerala, India.
authors.forename: Nafeesathul
authors.initials: N
authors.lastname: Miziriya
authors.affiliation: Department of Biotechnology, Cochin University of Science and Technology, Cochin-22, Kerala, India.
authors.forename: P S
authors.initials: PS
authors.lastname: Soumya
authors.affiliation: Department of Biotechnology, Cochin University of Science and Technology, Cochin-22, Kerala, India.
authors.forename: M S Kiran
authors.initials: MS
authors.lastname: Lakshmi
authors.affiliation: Department of Biotechnology, Cochin University of Science and Technology, Cochin-22, Kerala, India.
authors.forename: Anala
authors.initials: A
authors.lastname: Ramgopal
authors.affiliation: Department of Biotechnology, Cochin University of Science and Technology, Cochin-22, Kerala, India.
authors.forename: Anuja
authors.initials: A
authors.lastname: Dileep
authors.affiliation: Department of Biotechnology, Cochin University of Science and Technology, Cochin-22, Kerala, India. plantbiotech1992@gmail.com.
authors.forename: Padma
authors.initials: P
authors.lastname: Nambisan
mesh_terms: D001487:Basidiomycota / Q000166:cytology* / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology* / Q000592:standards; D049109:Cell Proliferation / Q000502:physiology; D003198:Computer Simulation; D003470:Culture Media / Q000378:metabolism / Q000592:standards; D005690:Galactose / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007194:India; D007218:Industrial Microbiology / Q000379:methods / Q000592:standards; D042845:Laccase / Q000096:biosynthesis* / Q000302:isolation & purification; D008954:Models, Biological; D015233:Models, Statistical; D011786:Quality Control; D013696:Temperature
chemical_list: D003470:Culture Media; D042845:Laccase; D005690:Galactose
keywords: Laccase; Marasmiellus palmivorus LA1; Optimization; Solid state fermentation; Taguchi DOE