title: Automated Trapping Column Exchanger for High-Throughput Nanoflow Liquid Chromatography.
abstract: As compared to conventional high-performance liquid chromatography (HPLC) techniques, nanoflow HPLC exhibits improved sensitivity and limits of detection. However, nanoflow HPLC suffers from low throughput due to instrument failure (e.g., fitting fatigue and trapping column failure), limiting the utility of the technique for clinical and industrial applications. To increase the robustness of nanoflow HPLC, we have developed and tested a trapping column exchanging robot for autonomous interchange of trapping columns. This robot makes reproducible, automated connections between the active trapping column and the rest of the HPLC system. The intertrapping column retention time is shown to be sufficiently reproducible for scheduled selected reaction monitoring assays to be performed on different trapping columns without rescheduling the selection windows.
authors.affiliation: Department of Genome Sciences, University of Washington School of Medicine , Box 355065, Seattle, Washington 98195-5065, United States.
authors.forename: Sandra E
authors.identifier: 0000-0001-6446-1584
authors.initials: SE
authors.lastname: Spencer
authors.affiliation: CorSolutions, LLC. , Cornell Business and Technology Park, 95 Brown Road, Box 1007, Ithaca, New York 14850-1294, United States.
authors.forename: Thomas N
authors.initials: TN
authors.lastname: Corso
authors.affiliation: Department of Genome Sciences, University of Washington School of Medicine , Box 355065, Seattle, Washington 98195-5065, United States.
authors.forename: James G
authors.initials: JG
authors.lastname: Bollinger
authors.affiliation: Department of Laboratory Medicine, University of Washington School of Medicine , Box 357110, Seattle, Washington 98195-7110, United States.
authors.forename: Clark M
authors.initials: CM
authors.lastname: Henderson
authors.affiliation: Department of Laboratory Medicine, University of Washington School of Medicine , Box 357110, Seattle, Washington 98195-7110, United States.
authors.forename: Andrew N
authors.initials: AN
authors.lastname: Hoofnagle
authors.affiliation: Department of Genome Sciences, University of Washington School of Medicine , Box 355065, Seattle, Washington 98195-5065, United States.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: MacCoss
mesh_terms: D000595:Amino Acid Sequence; D001331:Automation; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D017076:Computer-Aided Design; D059788:Dried Blood Spot Testing; D006801:Humans; D036103:Nanotechnology; D010455:Peptides / Q000032:analysis / Q000097:blood / Q000737:chemistry; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
chemical_list: D010455:Peptides