title: First record and description of juvenile stages of Longidorus artemisiae Rubtsova, Chizhov & Subbotin, 1999 (Nematoda: Longidoridae) in Poland and new data on L. juglandicola Lišková, Robbins & Brown, 1997 based on topotype specimens from Slovakia.
abstract: This paper presents the first geographical record of the needle nematode Longidorus artemisiae Rubtsova, Chizhov & Subbotin, 1999 outside Russia. This species was found in Poland near the city of Skierniewice in association with nettle (Urtica dioica L.). Morphometric and morphological data are provided, including the first description of juveniles of this species. Nematodes of the Polish population differ from the type-population in Russia in possessing a thicker body (lower 'a' index) in both sexes; males having a longer body and longer spicules; different sex ratio (1:2 in Polish population vs 1:1 in the type-population) and somewhat less expanded lips. Molecularly, the Polish population was characterised by sequencing D2-D3 28S rDNA and ITS1 markers. Additionally, new data on these two markers are provided for another species, Longidorus juglandicola Lišková, Robbins & Brown, 1997, obtained from topotype specimens from Slovakia. Surprisingly, despite the high morphological similarity of these two species, analysis of their phylogenetic position did not show close phylogenetic relation and several other species (less similar in general morphology) appeared more closely related to both L. artemisiae and L. juglandicola.
authors.affiliation: Department of Zoology, Institute of Plant Protection - National Research Institute, Władysława Węgorka 20, 60-318, Poznan, Poland. f.kornobis@onet.eu.
authors.forename: Franciszek Wojciech
authors.initials: FW
authors.lastname: Kornobis
authors.affiliation: Department of Environmental and Plant Parasitology, Institute of Parasitology SAS, Hlinkova 3, 040 01, Košice, Slovak Republic.
authors.forename: Marek
authors.initials: M
authors.lastname: Renčo
authors.affiliation: Department of Biological Pest Control, Institute of Plant Protection - National Research Institute, Władysława Węgorka 20, 60-318, Poznan, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Filipiak
mesh_terms: D000818:Animals; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005260:Female; D008297:Male; D009348:Nematoda / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics; D010802:Phylogeny; D018517:Plant Roots / Q000469:parasitology; D011044:Poland; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D018154:Slovakia; D012987:Soil / Q000469:parasitology; D013045:Species Specificity; D026982:Urtica dioica / Q000469:parasitology
chemical_list: D021903:DNA, Ribosomal Spacer; D012339:RNA, Ribosomal, 28S; D012987:Soil