title: iTRAQ reveals proteomic changes during intestine regeneration in the sea cucumber Apostichopus japonicus.
abstract: Sea cucumbers have a striking capacity to regenerate most of their viscera after evisceration, which has drawn the interest of many researchers. In this study, the isobaric tag for relative and absolute quantitation (iTRAQ) was utilized to investigate protein abundance changes during intestine regeneration in sea cucumbers. A total of 4073 proteins were identified, and 2321 proteins exhibited significantly differential expressions, with 1100 upregulated and 1221 downregulated proteins. Our results suggest that intestine regeneration constitutes a complex life activity regulated by the cooperation of various biological processes, including cytoskeletal changes, extracellular matrix (ECM) remodeling and ECM-receptor interactions, protein synthesis, signal recognition and transduction, energy production and conversion, and substance transport and metabolism. Additionally, real-time PCR showed mRNA expression of differentially expressed genes correlated positively with their protein levels. Our results provided a basis for studying the regulatory mechanisms associated with sea cucumber regeneration.
authors.affiliation: Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Sun
authors.affiliation: Marine Science and Engineering College, Qingdao Agricultural University, Qingdao, China.
authors.forename: Dongxue
authors.initials: D
authors.lastname: Xu
authors.affiliation: Key Laboratory of Marine Ecology and Environmental Science and Engineering, First Institute of Oceanography, State Oceanic Administration, Qingdao, China.
authors.forename: Qinzeng
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.
authors.forename: Jingchun
authors.initials: J
authors.lastname: Sun
authors.affiliation: Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China; University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Xing
authors.affiliation: Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China. Electronic address: zhanglibin@qdio.ac.cn.
authors.forename: Libin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Yang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000379:methods; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000166:cytology*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D012038:Regeneration / Q000502:physiology*; D012616:Sea Cucumbers / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D015415:Biomarkers; D020543:Proteome
keywords: Intestine regeneration; Proteomics; Sea cucumber; iTRAQ