title: An autonomous lab on a chip for space flight calibration of gravity-induced transcellular calcium polarization in single-cell fern spores.
abstract: This report describes the development of lab-on-a-chip device designed to measure changes in cellular ion gradients that are induced by changes in gravitational (g) forces. The bioCD presented here detects differential calcium ion concentrations outside of individual cells. The device includes sufficient replicates for statistical analysis of the gradients around multiple single cells and around control wells that are empty or include dead cells. In the data presented, the degree of the cellular response correlates with the magnitude of the g-force applied via rotation of the bioCD. The experiments recorded the longest continuous observation of a cellular response to hypergravity made to date, and they demonstrate the potential utility of this device for assaying the threshold of cells' g-force responses in spaceflight conditions.
authors.affiliation: Agricultural & Biological Engineering, Physiological Sensing Facility at the Bindley Bioscience Center & Birck Nanotechnology Center, Purdue University, West Lafayette, IN 47907, USA. rickus@purdue.edu.
authors.forename: J
authors.identifier: 0000-0003-1744-0099
authors.initials: J
authors.lastname: Park
authors.affiliation: Molecular Biosciences, The University of Texas at Austin, 1 University Station A6700, Austin, TX 78712, USA.
authors.forename: M L
authors.identifier: 0000-0002-1812-8876
authors.initials: ML
authors.lastname: Salmi
authors.affiliation: Agricultural & Biological Engineering, Physiological Sensing Facility at the Bindley Bioscience Center & Birck Nanotechnology Center, Purdue University, West Lafayette, IN 47907, USA. rickus@purdue.edu.
authors.forename: W W A
authors.initials: WW
authors.lastname: Wan Salim
authors.affiliation: NASA Ames Research Center, Moffett Field, CA 94035, USA.
authors.forename: A
authors.initials: A
authors.lastname: Rademacher
authors.affiliation: NASA Ames Research Center, Moffett Field, CA 94035, USA.
authors.forename: B
authors.initials: B
authors.lastname: Wickizer
authors.affiliation: NASA Ames Research Center, Moffett Field, CA 94035, USA.
authors.forename: A
authors.initials: A
authors.lastname: Schooley
authors.affiliation: NASA Ames Research Center, Moffett Field, CA 94035, USA.
authors.forename: J
authors.initials: J
authors.lastname: Benton
authors.affiliation: Molecular Biosciences, The University of Texas at Austin, 1 University Station A6700, Austin, TX 78712, USA.
authors.forename: A
authors.initials: A
authors.lastname: Cantero
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: P F
authors.initials: PF
authors.lastname: Argote
authors.affiliation: Dept of Statistics, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: M
authors.initials: M
authors.lastname: Ren
authors.affiliation: Dept of Statistics, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: M
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Agricultural & Biological Engineering, Physiological Sensing Facility at the Bindley Bioscience Center & Birck Nanotechnology Center, Purdue University, West Lafayette, IN 47907, USA. rickus@purdue.edu and Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: D M
authors.initials: DM
authors.lastname: Porterfield
authors.affiliation: NASA Ames Research Center, Moffett Field, CA 94035, USA.
authors.forename: A J
authors.initials: AJ
authors.lastname: Ricco
authors.affiliation: Molecular Biosciences, The University of Texas at Austin, 1 University Station A6700, Austin, TX 78712, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Roux
authors.affiliation: Agricultural & Biological Engineering, Physiological Sensing Facility at the Bindley Bioscience Center & Birck Nanotechnology Center, Purdue University, West Lafayette, IN 47907, USA. rickus@purdue.edu and Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: J L
authors.identifier: 0000-0002-6325-330X
authors.initials: JL
authors.lastname: Rickus
mesh_terms: D057205:Automation, Laboratory; D002118:Calcium / Q000737:chemistry / Q000378:metabolism*; D002138:Calibration; D004867:Equipment Design; D029624:Ferns / Q000737:chemistry / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D006112:Gravitation; D056656:Lab-On-A-Chip Devices; D012399:Rotation; D013026:Space Flight / Q000295:instrumentation*; D013170:Spores / Q000737:chemistry / Q000166:cytology / Q000378:metabolism / Q000502:physiology*
chemical_list: D002118:Calcium