title: Arachidonic acid triggers [Ca2+]i increases in rat round spermatids by a likely GPR activation, ERK signalling and ER/acidic compartments Ca2+ release.
abstract: Arachidonic acid (AA), a compound secreted by Sertoli cells (SC) in a FSH-dependent manner, is able to induce the release of Ca2+ from internal stores in round spermatids and pachytene spermatocytes. In this study, the possible site(s) of action of AA in round spermatids, the signalling pathways associated and the intracellular Ca2+ stores targeted by AA-induced signalling were pharmacologically characterized by measuring intracellular Ca2+ using fluorescent Ca2+ probes. Our results suggest that AA acts by interacting with a fatty acid G protein coupled receptor, initiating a G protein signalling cascade that may involve PLA2 and ERK activation, which in turn opens intracellular ryanodine-sensitive channels as well as NAADP-sensitive channels in acidic intracellular Ca2+ stores. The results presented here also suggest that AMPK and PKA modulate this AA-induced Ca2+ release from intracellular Ca2+ stores in round spermatids. We propose that unsaturated free fatty acid lipid signalling in the seminiferous tubule is a novel regulatory component of rat spermatogenesis.
authors.affiliation: Instituto de Química, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.
authors.forename: Joaquin
authors.initials: J
authors.lastname: Paillamanque
authors.affiliation: Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Autónoma de México, Cuernavaca, México.
authors.forename: Ana
authors.initials: A
authors.lastname: Sanchez-Tusie
authors.affiliation: Instituto de Química, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.
authors.forename: Emerson M
authors.initials: EM
authors.lastname: Carmona
authors.affiliation: Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Autónoma de México, Cuernavaca, México.
authors.forename: Claudia L
authors.initials: CL
authors.lastname: Treviño
authors.affiliation: Instituto de Química, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.
authors.forename: Carolina
authors.initials: C
authors.lastname: Sandoval
authors.affiliation: Departamento de Biología Celular, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, Chile.
authors.forename: Francisco
authors.initials: F
authors.lastname: Nualart
authors.affiliation: Instituto de Química, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.
authors.forename: Nelson
authors.initials: N
authors.lastname: Osses
authors.affiliation: Instituto de Química, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.
authors.forename: Juan G
authors.identifier: http://orcid.org/0000-0002-3274-3605
authors.initials: JG
authors.lastname: Reyes
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004721:Endoplasmic Reticulum / Q000187:drug effects* / Q000378:metabolism; D011992:Endosomes / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D008297:Male; D018613:Microscopy, Confocal; D009249:NADP / Q000031:analogs & derivatives / Q000378:metabolism; D054467:Phospholipases A2 / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D043562:Receptors, G-Protein-Coupled / Q000819:agonists* / Q000378:metabolism; D012459:Salicylates / Q000494:pharmacology; D054830:Sesterterpenes / Q000494:pharmacology; D013087:Spermatids / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013737:Testis / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
chemical_list: C533284:Ffar4 protein, rat; D043562:Receptors, G-Protein-Coupled; D012459:Salicylates; D054830:Sesterterpenes; C000597016:grifolic acid methyl ether; C531084:nicotinic acid adenine dinucleotide phosphate acetoxymethyl ester; D016718:Arachidonic Acid; D009249:NADP; D054467:Phospholipases A2; D002118:Calcium