title: Systemic innate immune activation in food protein-induced enterocolitis syndrome.
abstract: BACKGROUND
  Food protein-induced enterocolitis syndrome (FPIES) is a non-IgE-mediated food allergy of infancy whose pathophysiology is poorly understood.
  
  
  OBJECTIVE
  We set out to identify and phenotype allergen-responsive cells in peripheral blood of a cohort of subjects undergoing supervised food challenge for FPIES.
  
  
  METHODS
  We profiled antigen-responsive cells in PBMCs by flow cytometry, and examined cells in whole blood obtained before and after challenge by CyTOF mass cytometry and RNAseq.
  
  
  RESULTS
  Using a CD154-based detection approach, we observed that milk, soy, or rice-responsive T cells, and TNF-α-producing CD154+T cells, were significantly lower in those with outgrown FPIES compared with those with active FPIES. However, levels were within the normal range and were inconsistent with a role in the pathophysiology of FPIES. Profiling of whole blood by CyTOF demonstrated profound activation of cells of the innate immune system after food challenge, including monocytes, neutrophils, natural killer cells, and eosinophils. Activation was not observed in children with outgrown FPIES. We confirmed this pattern of innate immune activation in a larger cohort by RNAseq. Furthermore, we observed pan-T-cell activation and redistribution from the circulation after a positive food challenge but not in those who had outgrown their FPIES.
  
  
  CONCLUSIONS
  Our data demonstrate a compelling role of systemic innate immune activation in adverse reactions elicited by foods in FPIES. Further investigation is needed to identify the mechanism of antigen specificity of adverse reactions to foods in FPIES.
authors.affiliation: Jaffe Food Allergy Institute, New York, NY.
authors.forename: Ritobrata
authors.initials: R
authors.lastname: Goswami
authors.affiliation: Jaffe Food Allergy Institute, New York, NY.
authors.forename: Ana Belen
authors.initials: AB
authors.lastname: Blazquez
authors.affiliation: Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY.
authors.forename: Roman
authors.initials: R
authors.lastname: Kosoy
authors.affiliation: Human Immune Monitoring Core, Icahn School of Medicine at Mount Sinai, New York, NY.
authors.forename: Adeeb
authors.initials: A
authors.lastname: Rahman
authors.affiliation: Jaffe Food Allergy Institute, New York, NY.
authors.forename: Anna
authors.initials: A
authors.lastname: Nowak-Węgrzyn
authors.affiliation: Jaffe Food Allergy Institute, New York, NY. Electronic address: cecilia.berin@mssm.edu.
authors.forename: M Cecilia
authors.initials: MC
authors.lastname: Berin
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology; D000818:Animals; D002648:Child; D002675:Child, Preschool; D004760:Enterocolitis / Q000276:immunology*; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology*; D006801:Humans; D007113:Immunity, Innate; D007223:Infant; D007958:Leukocyte Count; D007962:Leukocytes / Q000166:cytology / Q000276:immunology; D008297:Male; D013577:Syndrome; D013601:T-Lymphocytes / Q000276:immunology; D055815:Young Adult
chemical_list: D000485:Allergens
keywords: CyTOF; FPIES; Food allergy; RNA-sequencing; innate; non-IgE