title: Bioactive Peptides Isolated from Casein Phosphopeptides Enhance Calcium and Magnesium Uptake in Caco-2 Cell Monolayers.
abstract: The ability of casein phosphopeptides (CPPs) to bind and transport minerals has been previously studied. However, the single bioactive peptides responsible for the effects of CPPs have not been identified. This study was to purify calcium-binding peptides from CPPs and to determine their effects on calcium and magnesium uptake by Caco-2 cell monolayers. Five monomer peptides designated P1 to P5 were isolated and the amino acid sequences were determined using LC-MS/MS. Compared with the CPP-free control, all five monomeric peptides exhibited significant enhancing effects on the uptake of calcium and magnesium (P < 0.05). Interestingly, when calcium and magnesium were presented simultaneously with P5, magnesium was taken up with priority over calcium in the Caco-2 cell monolayers. For example, at 180 min, the amount of transferred magnesium and calcium was 78.4 ± 0.95 μg/well and 2.56 ± 0.64 μg/well, respectively, showing a more than 30-fold difference in the amount of transport caused by P5. These results provide novel insight into the mineral transport activity of phosphopeptides obtained from casein.
authors.affiliation: College of Food Science, South China Agricultural University , Guangzhou 510642, People's Republic of China.
authors.forename: Yong
authors.identifier: http://orcid.org/0000-0001-8616-9525
authors.initials: Y
authors.lastname: Cao
authors.affiliation: College of Food Science, South China Agricultural University , Guangzhou 510642, People's Republic of China.|Department of Food Science, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: Jianyin
authors.initials: J
authors.lastname: Miao
authors.affiliation: College of Food Science, South China Agricultural University , Guangzhou 510642, People's Republic of China.
authors.forename: Guo
authors.initials: G
authors.lastname: Liu
authors.affiliation: Infinitus (China) Company Ltd. , Guangzhou, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Luo
authors.affiliation: Infinitus (China) Company Ltd. , Guangzhou, People's Republic of China.
authors.forename: Zumeng
authors.initials: Z
authors.lastname: Xia
authors.affiliation: Guangzhou Greencream Biotech Co., Ltd. , Guangzhou, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Food Science, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: Mingfei
authors.initials: M
authors.lastname: Yao
authors.affiliation: Department of Food Science, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: Xiaoqiong
authors.initials: X
authors.lastname: Cao
authors.affiliation: College of Food Science, South China Agricultural University , Guangzhou 510642, People's Republic of China.
authors.forename: Shengwei
authors.initials: S
authors.lastname: Sun
authors.affiliation: College of Food Science, South China Agricultural University , Guangzhou 510642, People's Republic of China.
authors.forename: Yanyin
authors.initials: Y
authors.lastname: Lin
authors.affiliation: College of Food Science, South China Agricultural University , Guangzhou 510642, People's Republic of China.|Department of Food Science, Rutgers University , 65 Dudley Road, New Brunswick, New Jersey 08901, United States.
authors.forename: Yaqi
authors.initials: Y
authors.lastname: Lan
authors.affiliation: Department of Food Science, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: Hang
authors.identifier: http://orcid.org/0000-0001-6194-2796
authors.initials: H
authors.lastname: Xiao
mesh_terms: D000595:Amino Acid Sequence; D001692:Biological Transport / Q000187:drug effects; D018938:Caco-2 Cells; D002118:Calcium / Q000378:metabolism*; D002364:Caseins / Q000737:chemistry*; D006801:Humans; D008274:Magnesium / Q000378:metabolism*; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D010748:Phosphopeptides / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D002364:Caseins; D010455:Peptides; D010748:Phosphopeptides; D008274:Magnesium; D002118:Calcium
keywords: Caco-2 cell monolayers; calcium transport; casein phosphopeptides; identification; magnesium transport