title: Comparative metabolite and genome analysis of tuber-bearing potato species.
abstract: The cultivated potato Solanum tuberosum is unrivalled among crop plants for its wild relatives, which potentially represent an important source of genetic diversity to improve the nutritional value of potato varieties and understand metabolism regulation. The main aim of this research was to profile human health-related metabolites in a number of clones from 13 Solanum species. Results from HPLC-DAD and LC-ESI-MS analyses highlighted a high interspecific variability in the level of metabolites analysed. Ascorbic acid was confirmed to be the most abundant antioxidant in potato and chlorogenic acid the primary polyphenol. Generally, metabolite-based hierarchical clustering (HCL) and correlation networks did not group clones of identical species in the same cluster. This might be due to various factors, including the outcrossing nature of potato species, gene expression level and metabolic profiling techniques. Access to the genome sequence of S. tuberosum and S. commersonii allowed comparison of the genes involved in ascorbic acid, aromatic amino acid, phenylpropanoid and glycoalkaloid biosynthesis and helped interpret their respective pathways.
authors.affiliation: Department of Agricultural Sciences, University of Naples Federico II, Portici 80055, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Aversano
authors.affiliation: Department of Agricultural Sciences, University of Naples Federico II, Portici 80055, Italy.
authors.forename: Felice
authors.initials: F
authors.lastname: Contaldi
authors.affiliation: Department of Agricultural Sciences, University of Naples Federico II, Portici 80055, Italy.
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Adelfi
authors.affiliation: Department of Agricultural Sciences, University of Naples Federico II, Portici 80055, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: D'Amelia
authors.affiliation: Italian National Agency for New Technologies, Energy, and Sustainable Development, Casaccia Research Centre, Rome 00123, Italy.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Diretto
authors.affiliation: Department of Pharmaceutical Sciences, University of Salerno, Via Ponte Don Melillo 1, 84084 Fisciano, Italy.
authors.forename: Nunziatina
authors.initials: N
authors.lastname: De Tommasi
authors.affiliation: Department of Pharmaceutical Sciences, University of Salerno, Via Ponte Don Melillo 1, 84084 Fisciano, Italy.
authors.forename: Carmen
authors.initials: C
authors.lastname: Vaccaro
authors.affiliation: Department of Science, University of Basilicata, Viale dell'Ateneo Lucano 10, 85100 Potenza, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Vassallo
authors.affiliation: Department of Agricultural Sciences, University of Naples Federico II, Portici 80055, Italy. Electronic address: carputo@unina.it.
authors.forename: Domenico
authors.initials: D
authors.lastname: Carputo
mesh_terms: D001205:Ascorbic Acid / Q000737:chemistry; D002726:Chlorogenic Acid / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D014644:Genetic Variation; D018745:Genome, Plant; D055442:Metabolome; D035281:Plant Tubers / Q000737:chemistry / Q000235:genetics; D032322:Solanum / Q000235:genetics* / Q000378:metabolism*; D011198:Solanum tuberosum / Q000235:genetics* / Q000378:metabolism*; D013045:Species Specificity; D053719:Tandem Mass Spectrometry; D014364:Tryptophan / Q000737:chemistry; D014443:Tyrosine / Q000737:chemistry
chemical_list: D002726:Chlorogenic Acid; D014443:Tyrosine; D014364:Tryptophan; D001205:Ascorbic Acid
keywords: Biodiversity; Biofortification; Comparative genomics; Polyphenols; Solanum spp. (Solanaceae)