title: Adipogenic niches for melanoma cell colonization and growth in bone marrow.
abstract: Bone marrow (BM) adipocytes are abundant in BM and may be involved in the process of bone metastasis. However, their behaviors in metastatic BM niches during bone metastasis have not been fully explored. In this study, intracardiac transplantation of B16-F10 melanoma cells into immunocompetent C57BL/6 mice was performed. Tibial marrow sections were stained with hematoxylin and eosin, Masson's trichrome, tartrate-resistant acid phosphatase, and fatty acid-binding protein 4 (FABP4) and analyzed using a histomorphometric system. The results showed that the number of BM adipocytes rapidly increased in melanoma metastatic BM niches, which were in direct contact with metastasizing melanoma cells and acted as a tumor stromal population in the BM-melanoma niche. Melanoma cell-derived factors could enhance BM adipogenesis, which promotes melanoma cell proliferation and cell cycle transitions. Moreover, BM adipocytes might aid in the modification of the osteolytic BM microenvironment. These results indicate that an increase in the number of BM adipocytes in a metastatic BM niche may facilitate melanoma cell colonization and growth in BM. BM adipocytes might therefore support the development of bone metastases.
authors.affiliation: Department of Internal Medicine, Shanghai First People's Hospital, Affiliated to Shanghai Jiao Tong University, Shanghai, China.|Department of Rheumatology, Renji Hospital, Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Internal Medicine 3, University of Erlangen-Nuremberg, Erlangen, Germany.|Department of Oncology, Minhang Hospital, Fudan University, Shanghai, China.
authors.forename: Guang-Liang
authors.initials: GL
authors.lastname: Chen
authors.affiliation: Department of Rheumatology, Renji Hospital, Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Cao
authors.affiliation: Department of Pathology, Guilin People's Hospital, Guilin, China.
authors.forename: Ming-Chun
authors.initials: MC
authors.lastname: Zhao
authors.affiliation: Department of Pathology, Guigang City hospital of Traditional Chinese Medicine, Guigang, China.
authors.forename: Yong-Qing
authors.initials: YQ
authors.lastname: Liu
authors.affiliation: Department of Rheumatology, Renji Hospital, Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai, China.
authors.forename: Xiao-Xiang
authors.initials: XX
authors.lastname: Chen
authors.affiliation: Department of Thoracic Surgery, Zhongshan hospital, Fudan University, Shanghai, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Qian
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000502:physiology; D050156:Adipogenesis / Q000502:physiology*; D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D001854:Bone Marrow Cells / Q000166:cytology*; D049109:Cell Proliferation; D008546:Melanoma, Experimental / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D055153:Stem Cell Niche / Q000502:physiology*; D059016:Tumor Microenvironment / Q000502:physiology*