title: Expression of Cadherin-17 Promotes Metastasis in a Highly Bone Marrow Metastatic Murine Breast Cancer Model.
abstract: We previously established 4T1E/M3 highly bone marrow metastatic mouse breast cancer cells throughin vivoselection of 4T1 cells. But while the incidence of bone marrow metastasis of 4T1E/M3 cells was high (~80%) when injected intravenously to mice, it was rather low (~20%) when injected subcutaneously. Therefore, using 4T1E/M3 cells, we carried out furtherin vitroandin vivoselection steps to establish FP10SC2 cells, which show a very high incidence of metastasis to lungs (100%) and spines (85%) after subcutaneous injection into mice. qRT-PCR and western bolt analysis revealed that cadherin-17 gene and protein expression were higher in FP10SC2 cells than in parental 4T1E/M3 cells. In addition, immunostaining revealed the presence of cadherin-17 at sites of bone marrow and lung metastasis after subcutaneous injection of FP10SC2 cells into mice. Suppressing cadherin-17 expression in FP10SC2 cells using RNAi dramatically decreased the cells' anchorage-independent growth and migrationin vitroand their metastasis to lung and bone marrowin vivo. These findings suggest that cadherin-17 plays a crucial role in mediating breast cancer metastasis to bone marrow.
authors.affiliation: Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Tomoko
authors.identifier: 0000-0003-3723-1147
authors.initials: T
authors.lastname: Okada
authors.affiliation: Department of Pathology, Kochi Medical School, Nankoku, Kochi 783-8505, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kurabayashi
authors.affiliation: Radioisotope Center, The University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan.
authors.forename: Nobuyoshi
authors.identifier: 0000-0002-3190-2942
authors.initials: N
authors.lastname: Akimitsu
authors.affiliation: Department of Pathology, Kochi Medical School, Nankoku, Kochi 783-8505, Japan.
authors.forename: Mutsuo
authors.initials: M
authors.lastname: Furihata
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000473:pathology; D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology / Q000556:secondary; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D015820:Cadherins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D002448:Cell Adhesion / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology / Q000556:secondary; D051379:Mice; D034622:RNA Interference
chemical_list: D015820:Cadherins