title: Evaluation the urban atmospheric conditions in different cities using comet and micronuclei assay in Tradescantia pallida.
abstract: In the present study, genotoxicity and mutagenicity were investigated in Tradescantia pallida exposed to vehicular traffic at different sites in a high-altitude tropical climate. During March, May, July, September, and November 2014, a comet assay and micronucleus bioassays were conducted on young inflorescences and leaves of T. pallida collected from twelve towns in the southern region of Mato Grosso do Sul with different amounts of vehicular traffic. Weather parameters (temperature, relative humidity and rainfall) were measured and vehicles were counted to determine traffic levels in each town. A higher frequency of genotoxic and mutagenic damage was observed in the municipality of Dourados. The highest frequency of genetic damage was observed in September and November according to both assays. Relative humidity and rainfall were inversely proportional to the frequency of genetic damage in T. pallida during the collection period. Based on these results, we conclude that the bioassays are efficient for assessing the effects of vehicular traffic in these towns with respect to weather conditions over time. These bioassays can be applied to identify risk areas, which are determined by climatic conditions and air pollutants released.
authors.affiliation: Faculty of Exact Sciences and Technology, Federal University of Grande Dourados/UFGD, Street João Rosa Góes, Vila Progresso, 79.825-070 Dourados, MS, Brazil. Electronic address: juliana_sposito@hotmail.com.
authors.forename: Juliana Caroline Vivian
authors.initials: JCV
authors.lastname: Sposito
authors.affiliation: Faculty of Exact Sciences and Technology, Federal University of Grande Dourados/UFGD, Street João Rosa Góes, Vila Progresso, 79.825-070 Dourados, MS, Brazil. Electronic address: bruno_cpo15@hotmail.com.
authors.forename: Bruno do Amaral
authors.initials: BDA
authors.lastname: Crispim
authors.affiliation: Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados/UFGD, Street João Rosa Góes, Vila Progresso, 79.825-070 Dourados, MS, Brazil. Electronic address: amandaroman_ms@hotmail.com.
authors.forename: Amanda Izadora
authors.initials: AI
authors.lastname: Romãn
authors.affiliation: Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados/UFGD, Street João Rosa Góes, Vila Progresso, 79.825-070 Dourados, MS, Brazil. Electronic address: maramussury@ufgd.edu.br.
authors.forename: Rosilda Mara
authors.initials: RM
authors.lastname: Mussury
authors.affiliation: Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados/UFGD, Street João Rosa Góes, Vila Progresso, 79.825-070 Dourados, MS, Brazil. Electronic address: joelsonpereira@ufgd.edu.br.
authors.forename: Joelson Gonçalves
authors.initials: JG
authors.lastname: Pereira
authors.affiliation: Faculty of Agricultural Sciences/FCA, Federal University of Grande Dourados/UFGD, Street João Rosa Góes, Vila Progresso, 79.825-070 Dourados, MS, Brazil. Electronic address: leonardoseno@ufgd.edu.br.
authors.forename: Leonardo Oliveira
authors.initials: LO
authors.lastname: Seno
authors.affiliation: Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados/UFGD, Street João Rosa Góes, Vila Progresso, 79.825-070 Dourados, MS, Brazil. Electronic address: alexeiagrisolia@ufgd.edu.br.
authors.forename: Alexeia Barufatti
authors.initials: AB
authors.lastname: Grisolia
mesh_terms: D000393:Air Pollutants / Q000009:adverse effects*; D001681:Biological Assay / Q000379:methods; D002947:Cities; D002980:Climate; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D004784:Environmental Monitoring / Q000379:methods*; D015162:Micronucleus Tests; D009153:Mutagens / Q000032:analysis; D018515:Plant Leaves / Q000187:drug effects; D034823:Tradescantia / Q000187:drug effects*; D001335:Vehicle Emissions / Q000633:toxicity*; D014887:Weather
chemical_list: D000393:Air Pollutants; D009153:Mutagens; D001335:Vehicle Emissions
keywords: Air pollution; Bioassay; Biomonitoring; Genetic damage; Genotoxic; Mutagenesis