title: Complete genome sequence ofPseudomonas stutzeristrain RCH2 isolated from a Hexavalent Chromium [Cr(VI)] contaminated site.
abstract: Hexavalent Chromium [Cr(VI)] is a widespread contaminant found in soil, sediment, and ground water in several DOE sites, including Hanford 100 H area. In order to stimulate microbially mediated reduction of Cr(VI) at this site, a poly-lactate hydrogen release compound was injected into the chromium contaminated aquifer. Targeted enrichment of dominant nitrate-reducing bacteria post injection resulted in the isolation ofPseudomonas stutzeristrain RCH2.P. stutzeristrain RCH2 was isolated using acetate as the electron donor and is a complete denitrifier. Experiments with anaerobic washed cell suspension of strain RCH2 revealed it could reduce Cr(VI) and Fe(III). The genome of strain RCH2 was sequenced using a combination of Illumina and 454 sequencing technologies and contained a circular chromosome of 4.6 Mb and three plasmids. Global genome comparisons of strain RCH2 with six other fully sequencedP. stutzeristrains revealed most genomic regions are conserved, however strain RCH2 has an additional 244 genes, some of which are involved in chemotaxis, Flp pilus biogenesis and pyruvate/2-oxogluturate complex formation.
authors.affiliation: Lawrence Berkeley National Laboratory, Berkeley, CA USA.
authors.forename: Romy
authors.identifier: 0000-0001-9326-554X
authors.initials: R
authors.lastname: Chakraborty
authors.affiliation: University Of Tennessee, Knoxville, TN USA.
authors.forename: Hannah
authors.initials: H
authors.lastname: Woo
authors.affiliation: Lawrence Berkeley National Laboratory, Berkeley, CA USA.
authors.forename: Paramvir
authors.initials: P
authors.lastname: Dehal
authors.affiliation: Lawrence Berkeley National Laboratory, Berkeley, CA USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Walker
authors.affiliation: Lawrence Berkeley National Laboratory, Berkeley, CA USA.
authors.forename: Marcin
authors.initials: M
authors.lastname: Zemla
authors.affiliation: Lawrence Berkeley National Laboratory, Berkeley, CA USA.
authors.forename: Manfred
authors.initials: M
authors.lastname: Auer
authors.affiliation: Department of Energy Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: Lynne A
authors.initials: LA
authors.lastname: Goodwin
authors.affiliation: Lawrence Berkeley National Laboratory, Berkeley, CA USA.
authors.forename: Alexey
authors.initials: A
authors.lastname: Kazakov
authors.affiliation: Lawrence Berkeley National Laboratory, Berkeley, CA USA.
authors.forename: Pavel
authors.initials: P
authors.lastname: Novichkov
authors.affiliation: Lawrence Berkeley National Laboratory, Berkeley, CA USA.
authors.forename: Adam P
authors.initials: AP
authors.lastname: Arkin
authors.affiliation: Lawrence Berkeley National Laboratory, Berkeley, CA USA.|University Of Tennessee, Knoxville, TN USA.
authors.forename: Terry C
authors.initials: TC
authors.lastname: Hazen
keywords: Chromium; Hanford 100H; Nitrate reduction; Pseudomonas