title: In Situ Bioorthogonal Metabolic Labeling for Fluorescence Imaging of Virus Infection In Vivo.
abstract: Optical fluorescence imaging is an important strategy to explore the mechanism of virus-host interaction. However, current fluorescent tag labeling strategies often dampen viral infectivity. The present study explores an in situ fluorescent labeling strategy in order to preserve viral infectivity and precisely monitor viral infection in vivo. In contrast to pre-labeling strategy, mice are first intranasally infected with azide-modified H5N1 pseudotype virus (N3-H5N1p), followed by injection of dibenzocyclooctyl (DBCO)-functionalized fluorescence 6 h later. The results show that DBCO dye directly conjugated to N3-H5N1p in lung tissues through in vivo bioorthogonal chemistry with high specificity and efficacy. More remarkably, in situ labeling rather than conventional prelabeling strategy effectively preserves viral infectivity and immunogenicity both in vitro and in vivo. Hence, in situ bioorthogonal viral labeling is a promising and reliable strategy for imaging and tracking viral infection in vivo.
authors.affiliation: Guangdong Key Laboratory of Nanomedicine, Key Lab of Health Informatics of Chinese Academy of Sciences, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, P. R. China.|School of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Pan
authors.affiliation: Guangdong Key Laboratory of Nanomedicine, Key Lab of Health Informatics of Chinese Academy of Sciences, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, P. R. China.|School of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
authors.affiliation: Major Infectious Disease Control Key Laboratory, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, P. R. China.
authors.forename: Xiang-Jie
authors.initials: XJ
authors.lastname: Yao
authors.affiliation: Guangdong Key Laboratory of Nanomedicine, Key Lab of Health Informatics of Chinese Academy of Sciences, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, P. R. China.|School of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors.forename: Ya-Yun
authors.initials: YY
authors.lastname: Wu
authors.affiliation: Guangdong Key Laboratory of Nanomedicine, Key Lab of Health Informatics of Chinese Academy of Sciences, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, P. R. China.|School of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors.forename: Lan-Lan
authors.initials: LL
authors.lastname: Liu
authors.affiliation: Guangdong Key Laboratory of Nanomedicine, Key Lab of Health Informatics of Chinese Academy of Sciences, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, P. R. China.|School of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors.forename: Hua-Mei
authors.initials: HM
authors.lastname: He
authors.affiliation: Major Infectious Disease Control Key Laboratory, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, P. R. China.
authors.forename: Ren-Li
authors.initials: RL
authors.lastname: Zhang
authors.affiliation: Guangdong Key Laboratory of Nanomedicine, Key Lab of Health Informatics of Chinese Academy of Sciences, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, P. R. China.|School of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors.forename: Yi-Fan
authors.initials: YF
authors.lastname: Ma
authors.affiliation: Guangdong Key Laboratory of Nanomedicine, Key Lab of Health Informatics of Chinese Academy of Sciences, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, P. R. China.|School of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors.forename: Lin-Tao
authors.initials: LT
authors.lastname: Cai
mesh_terms: D057930:Click Chemistry; D053124:Influenza A Virus, H5N1 Subtype / Q000472:pathogenicity*; D061848:Optical Imaging / Q000379:methods*
keywords: bioorthogonal chemistry; in vivo imaging; metabolic labeling; virus infection