title: Detection of soluble ERBB2 in breast cancer cell lysates using a combined label-free/fluorescence platform based on Bloch surface waves.
abstract: We report on the use of one-dimensional photonic crystals to detect clinically relevant concentrations of ERBB2/neu/Her2 in cell lysates. ERBB2 is a pivotal breast cancer biomarker and targetable oncogenic driver associated with aggressive breast cancer subtypes. To quantitate soluble ERBB2, we developed an optical platform that combines label-free and fluorescence detection modes. Such platform makes use of a sandwich assay in which the one-dimensional photonic crystals sustaining Bloch surface waves are tailored with a monoclonal antibody for highly specific biological recognition (BSW biochip). In a second step, a second antibody to ERBB2 quantitatively detects the bound analyte. The strategy of the present approach takes advantage of the combination of label-free and fluorescence techniques, making bio-recognition more robust and sensitive. In the fluorescence operation mode, the platform can attain the limit of detection 0.3ng/mL (1.5pM) for ERBB2 in cell lysates. Such resolution meets the international guidelines and recommendations (15ng/mL) for diagnostic ERBB2 assays that in the future may help to more precisely assign therapies counteracting cancer cell proliferation and metastatic spread.
authors.affiliation: Department of Basic and Applied Science for Engineering, Sapienza University of Rome, Via A. Scarpa 16, 00161 Rome, Italy. Electronic address: alberto.sinibaldi@uniroma1.it.
authors.forename: Alberto
authors.initials: A
authors.lastname: Sinibaldi
authors.affiliation: Regina Elena National Cancer Institute, Via Elio Chianesi 53, 00144 Rome, Italy.
authors.forename: Camilla
authors.initials: C
authors.lastname: Sampaoli
authors.affiliation: Fraunhofer Institute for Applied Optics and Precision Engineering IOF, Albert-Einstein-Str. 7, 07745 Jena, Germany.
authors.forename: Norbert
authors.initials: N
authors.lastname: Danz
authors.affiliation: Fraunhofer Institute for Applied Optics and Precision Engineering IOF, Albert-Einstein-Str. 7, 07745 Jena, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Munzert
authors.affiliation: IBI, Istituto Biochimico Italiano Giovanni Lorenzini Spa, Via Fossignano 2, 04011 Aprilia, Italy.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Sibilio
authors.affiliation: Fraunhofer Institute for Material and Beam Technology IWS, Winterbergstr. 28, 01277 Dresden, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Sonntag
authors.affiliation: Department of Basic and Applied Science for Engineering, Sapienza University of Rome, Via A. Scarpa 16, 00161 Rome, Italy.
authors.forename: Agostino
authors.initials: A
authors.lastname: Occhicone
authors.affiliation: Institute of Molecular Biology and Pathology, National Research Council, P.le Aldo Moro 5, 00185 Rome, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Falvo
authors.affiliation: Regina Elena National Cancer Institute, Via Elio Chianesi 53, 00144 Rome, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Tremante
authors.affiliation: Regina Elena National Cancer Institute, Via Elio Chianesi 53, 00144 Rome, Italy.
authors.forename: Patrizio
authors.initials: P
authors.lastname: Giacomini
authors.affiliation: Department of Basic and Applied Science for Engineering, Sapienza University of Rome, Via A. Scarpa 16, 00161 Rome, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Michelotti
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation*; D001940:Breast / Q000473:pathology*; D001943:Breast Neoplasms / Q000175:diagnosis*; D045744:Cell Line, Tumor; D004867:Equipment Design; D005260:Female; D005453:Fluorescence; D006801:Humans; D056656:Lab-On-A-Chip Devices; D057230:Limit of Detection; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human
keywords: 1D photonic crystals; Bloch surface waves; Breast cancer; ERBB2; Optical biosensors; SK-BR 3