title: Efficacy of Human Platelet Rich Fibrin Exudate vs Fetal Bovine Serum on Proliferation and Differentiation of Dental Pulp Stem Cells.
abstract: OBJECTIVE
  The imperative role of dental pulp stem cells (DPSCs) in regenerative therapy demands anin-vitroexpansion which must deal with the safety and ethical problems associated with fetal bovine serum (FBS). The primary aim of this study was to compare the effects of human platelet rich fibrin (hPRF) exudateVsFBS on proliferation and osteodifferentiation of human dental pulp stem cells (hDPSCs). The secondary one was to determine the optimum concentration of hPRF exudate inducing hDPSCs proliferation and osteodifferentiation.
  
  
  METHODS
  The direct method was used to prepare hPRF exudate. hDPSCs were isolated from impacted mandibular third molars of twelve donors by the outgrowth method. For cell viability and proliferation rate testing, 96 well plates were used and the assay was done in duplicate and the trial repeated four times under the same conditions. Six wells were used to contain 10% FBS, serum free media, 1%, 5%, 10% and 20% concentrations of hPRF exudates, respectively. The proliferation assay was carried out by MTS tetrazolium cell proliferation assay kit and Elisa reader. The study design for osteodifferentiation protocol was exactly as the proliferation one and instead the assay was carried out by alizarin red with Elisa reader.
  
  
  RESULTS
  Compared to 10% FBS, 10% hPRF exudate was the optimum concentration for hDPSCs proliferation, while 1% hPRF exudate was the optimum concentration for osteodifferentiation of hDPSCs. Conclusions: Avoiding the risk of zoonosis which may be occurred with FBS, it is recommended to use 10% hPRF exudate for proliferation and 1% for osteodifferentiation.
authors.affiliation: Department of Oral Biology, Faculty of Oral and Dental Medicine, Delta University for Science and Technology, Gamasa, Mansoura, Egypt.
authors.forename: Mahmoud A
authors.initials: MA
authors.lastname: Saeed
authors.affiliation: Department of Oral Biology, Faculty of Dentistry, Mansoura University, Mansoura, Egypt.
authors.forename: Mohamed Abd
authors.initials: MA
authors.lastname: El-Rahman
authors.affiliation: Department of Oral Biology, Faculty of Dentistry, Mansoura University, Mansoura, Egypt.
authors.forename: Mohamed E
authors.initials: ME
authors.lastname: Helal
authors.affiliation: Department of Oral Biology, Faculty of Dentistry, Mansoura University, Mansoura, Egypt.
authors.forename: Ahmed R
authors.initials: AR
authors.lastname: Zaher
authors.affiliation: Department of Oral Biology, Faculty of Oral and Dental Medicine, Delta University for Science and Technology, Gamasa, Mansoura, Egypt.|Department of Oral Biology, Faculty of Dentistry, Mansoura University, Mansoura, Egypt.
authors.forename: Mohammed E
authors.initials: ME
authors.lastname: Grawish
keywords: Alizarin Red S staining assay; Dental pulp stem cells; Fetal bovine serum; Human platelet rich fibrin exudate; MTS cell proliferation assay