title: Virulotyping of Shigella spp. isolated from pediatric patients in Tehran, Iran.
abstract: Shigellosis is a considerable infectious disease with high morbidity and mortality among children worldwide. In this survey the prevalence of four important virulence genes including ial, ipaH, set1A, and set1B were investigated among Shigella strains and the related gene profiles identified in the present investigation, stool specimens were collected from children who were referred to two hospitals in Tehran, Iran. The samples were collected during 3 years (2008-2010) from children who were suspected to shigellosis. Shigella spp. were identified throughout microbiological and serological tests and then subjected to PCR for virulotyping. Shigella sonnei was ranking first (65.5%) followed by Shigella flexneri (25.9%), Shigella boydii (6.9%), and Shigella dysenteriae (1.7%). The ial gene was the most frequent virulence gene among isolated bacterial strains and was followed by ipaH, set1B, and set1A. S. flexneri possessed all of the studied virulence genes (ial 65.51%, ipaH 58.62%, set1A 12.07%, and set1B 22.41%). Moreover, the pattern of virulence gene profiles including ial, ial-ipaH, ial-ipaH-set1B, and ial-ipaH-set1B-set1A was identified for isolated Shigella spp. strains. The pattern of virulence genes is changed in isolated strains of Shigella in this study. So, the ial gene is placed first and the ipaH in second.
authors.affiliation: 1 Molecular Biology Research Center, Baqiyatallah University of Medical Sciences , Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Ranjbar
authors.affiliation: 1 Molecular Biology Research Center, Baqiyatallah University of Medical Sciences , Tehran, Iran.
authors.forename: Masomeh
authors.initials: M
authors.lastname: Bolandian
authors.affiliation: 2 Department of Microbiology, College of Basic Sciences, Shahr-e-Qods Branch, Islamic Azad University , Tehran, Iran.
authors.forename: Payam
authors.initials: P
authors.lastname: Behzadi
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D004405:Dysentery, Bacillary / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D006801:Humans; D007492:Iran; D012760:Shigella / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D037521:Virulence Factors
keywords: PCR; Shigella; gene profile; shigellosis; virulence gene