title: In vivomapping of a dynamic ribonucleoprotein granule interactome in earlyDrosophilaembryos.
abstract: Macromolecular complexes and organelles play crucial roles within cells, but their native architectures are often unknown. Here, we use an evolutionarily conserved germline organelle, the germ granule, as a paradigm. InDrosophilaembryos, we map one of its interactomes using a novelin vivocrosslinking approach that employs two interacting granule proteins and determines their common neighbor molecules. We identified anin vivogranule assembly of Tudor, Aubergine, motor and metabolic proteins, and RNA helicases, and provide evidence for direct interactions within this assembly using purified components. Our study indicates that germ granules contain efficient biochemical reactors involved in post-transcriptional gene regulation.
authors.affiliation: Department of Biological Sciences Murray State University USA.
authors.forename: Jimiao
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Biology Department Indiana University Northwest Gary IN USA.
authors.forename: Ming
authors.initials: M
authors.lastname: Gao
authors.affiliation: Department of Biological Sciences Murray State University USA; Present address: University of Alberta Edmonton AB Canada.
authors.forename: Nhan
authors.initials: N
authors.lastname: Huynh
authors.affiliation: Department of Biological Sciences Murray State University USA.
authors.forename: Samuel J
authors.initials: SJ
authors.lastname: Tindell
authors.affiliation: Department of Biological Sciences Murray State University USA.
authors.forename: Hieu D L
authors.initials: HD
authors.lastname: Vo
authors.affiliation: Department of Biochemistry Mass Spectrometry Research Center Vanderbilt University School of Medicine Nashville TN USA.
authors.forename: W Hayes
authors.initials: WH
authors.lastname: McDonald
authors.affiliation: Department of Biological Sciences Murray State University USA.
authors.forename: Alexey L
authors.initials: AL
authors.lastname: Arkov
keywords: Drosophila; Piwi; Tudor; germ cells; germ granules; in vivo interactome mapping; organelle