title: Hematopoietic Stem Cell Transplantation Using Preimplantation Genetic Diagnosis and Human Leukocyte Antigen Typing for Human Leukocyte Antigen-Matched Sibling Donor: A Turkish Multicenter Study.
abstract: Preimplantation genetic diagnosis involves the diagnosis of a genetic disorder in embryos obtained through in vitro fertilization, selection of healthy embryos, and transfer of the embryos to the mother's uterus. Preimplantation genetic diagnosis has been used not only to avoid the risk of having an affected child, but it also offers, using HLA matching, preselection of potential HLA-genoidentical healthy donor progeny for an affected sibling who requires bone marrow transplantation. Here, we share the hematopoietic stem cell transplantation results of 52 patients with different benign and malign hematological or metabolic diseases or immunodeficiencies whose donors were siblings born with this technique in Turkey since 2008. The median age of the patients' at the time of the transplantation was 8 years (range, 3 to 16 years) and the median age of the donors was 2 years (range, .5 to 6 years). The most common indication for HSCT was thalassemia major (42 of all patients, 80%). The stem cell source in all of the transplantations was bone marrow. In 37 of the transplantations, umbilical cord blood of the same donor was also used. In 50 of the 52 patients, full engraftment was achieved with a mean of 4.6 × 106CD 34+cells per kg of recipient weight. Ninety-six percent of the patients have been cured through hematopoietic stem cell transplantation without any complication. Primary engraftment failure was seen in only 2 patients with thalassemia major. All of the donors and the patients are alive with good health status. Preimplantation genetic diagnosis with HLA matching offers a life-saving chance for patients who need transplantation but lack an HLA genoidentical donor.
authors.affiliation: Department of Pediatric Hematology, Lösante Hospital Ankara, Ankara, Turkey. Electronic address: ekurekci@gmail.com.
authors.forename: Emin
authors.initials: E
authors.lastname: Kurekci
authors.affiliation: Department of Pediatric Hematology and Oncology, Akdeniz University Medical Faculty, Antalya, Turkey.
authors.forename: Alphan
authors.initials: A
authors.lastname: Küpesiz
authors.affiliation: Department of Pediatric Hematology and Oncology, İstanbul University Medical Faculty, Istanbul, Turkey.
authors.forename: Sema
authors.initials: S
authors.lastname: Anak
authors.affiliation: Pediatric Bone Marrow Transplantation Unit, Medical Park Bahçelievler Hospital, Istanbul, Turkey.
authors.forename: Gülyüz
authors.initials: G
authors.lastname: Öztürk
authors.affiliation: Department of Pediatric Hematology, Gülhane Military Medical Academy and Medical Faculty, Ankara, Turkey.
authors.forename: Orhan
authors.initials: O
authors.lastname: Gürsel
authors.affiliation: Department of Pediatric Hematology and Oncology, Ege University Medical Faculty, Izmir, Turkey.
authors.forename: Serap
authors.initials: S
authors.lastname: Aksoylar
authors.affiliation: Department of Pediatric Hematology and Oncology, Ankara University Medical Faculty, Ankara, Turkey.
authors.forename: Talia
authors.initials: T
authors.lastname: Ileri
authors.affiliation: Department of Pediatric Hematology and Oncology, Hacettepe University Medical Faculty, Ankara, Turkey.
authors.forename: Barış
authors.initials: B
authors.lastname: Kuşkonmaz
authors.affiliation: Department of Pediatric Hematology, Gülhane Military Medical Academy and Medical Faculty, Ankara, Turkey.
authors.forename: İbrahim
authors.initials: İ
authors.lastname: Eker
authors.affiliation: Department of Pediatric Hematology and Oncology, Hacettepe University Medical Faculty, Ankara, Turkey.
authors.forename: Mualla
authors.initials: M
authors.lastname: Cetin
authors.affiliation: Bahçeşehir University Medical Faculty, Medical Park Göztepe Hospital, Istanbul, Turkey.
authors.forename: Gülsün
authors.initials: G
authors.lastname: Tezcan Karasu
authors.affiliation: Department of Pediatric Hematology and Oncology, Gazi University Medical Faculty, Ankara, Turkey.
authors.forename: Zühre
authors.initials: Z
authors.lastname: Kaya
authors.affiliation: Bahçeşehir University Medical Faculty, Medical Park Bahçelievler Hospital, Istanbul, Turkey.
authors.forename: Tunç
authors.initials: T
authors.lastname: Fışgın
authors.affiliation: Department of Pediatric Hematology and Oncology, Ankara University Medical Faculty, Ankara, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Ertem
authors.affiliation: Department of Pediatric Hematology and Oncology, Ege University Medical Faculty, Izmir, Turkey.
authors.forename: Savaş
authors.initials: S
authors.lastname: Kansoy
authors.affiliation: Turkish Pediatric Bone Marrow Transplantation Study Group. Bahçeşehir University Medical Faculty, Medical Park Göztepe Hospital, Istanbul, Turkey.
authors.forename: Mehmet Akif
authors.initials: MA
authors.lastname: Yeşilipek
mesh_terms: D000293:Adolescent; D016026:Bone Marrow Transplantation; D002648:Child; D002675:Child, Preschool; D036101:Cord Blood Stem Cell Transplantation; D005260:Female; D006085:Graft Survival; D006680:HLA Antigens / Q000032:analysis; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D011247:Pregnancy; D019836:Preimplantation Diagnosis; D035781:Siblings; D014019:Tissue Donors; D017086:beta-Thalassemia / Q000628:therapy*
chemical_list: D006680:HLA Antigens
keywords: Children; HLA matching; Preimplantation genetic diagnosis; Transplantation