title: Isolation and Characterization of Bacillus cereus Bacteriophages from Foods and Soil.
abstract: The aim of this study was to isolate and characterize Bacillus cereus bacteriophages of various origins. Twenty-seven bacteriophages against B. cereus were isolated from various Korean traditional fermented foods and soils. Plaque size, transmission electron microscopy, virulence profile, and in vitro lytic activity of bacteriophage isolates were examined. Transmission electron microscopy confirmed B. cereus bacteriophages belonging to the family Siphoviridae. Among B. cereus bacteriophages with broad host range, 18 isolates (66.7%) did not harbor any B. cereus virulence factors. Among them, bacteriophage strain CAU150036, CAU150038, CAU150058, CAU150064, CAU150065, and CAU150066 effectively inhibited B. cereus in vitro within 1 h. Therefore, they are considered potential candidates for controlling the contamination of B. cereus in food or other applications.
authors.affiliation: Department of Food and Nutrition, College of Biotechnology and Agriculture Resource, Chung-Ang University, Seodongdaero, Daeduck, Anseong, Gyeonggi, 17546, Republic of Korea.
authors.forename: Hyejin
authors.initials: H
authors.lastname: Oh
authors.affiliation: Department of Food and Nutrition, College of Biotechnology and Agriculture Resource, Chung-Ang University, Seodongdaero, Daeduck, Anseong, Gyeonggi, 17546, Republic of Korea.
authors.forename: Dong Joo
authors.initials: DJ
authors.lastname: Seo
authors.affiliation: Department of Food and Nutrition, College of Biotechnology and Agriculture Resource, Chung-Ang University, Seodongdaero, Daeduck, Anseong, Gyeonggi, 17546, Republic of Korea.
authors.forename: Su Been
authors.initials: SB
authors.lastname: Jeon
authors.affiliation: Department of Food and Nutrition, College of Biotechnology and Agriculture Resource, Chung-Ang University, Seodongdaero, Daeduck, Anseong, Gyeonggi, 17546, Republic of Korea.
authors.forename: Hyunkyung
authors.initials: H
authors.lastname: Park
authors.affiliation: Department of Food and Nutrition, College of Biotechnology and Agriculture Resource, Chung-Ang University, Seodongdaero, Daeduck, Anseong, Gyeonggi, 17546, Republic of Korea.
authors.forename: Suntak
authors.initials: S
authors.lastname: Jeong
authors.affiliation: Department of Food Science and Technology, College of Biotechnology and Agriculture Resource, Chung-Ang University, Anseong, Gyeonggi, 17546, Republic of Korea.
authors.forename: Hyang Sook
authors.initials: HS
authors.lastname: Chun
authors.affiliation: National Institute of Animal Science, Rural Development Administration, Jeonju, Jeollabuk-do, 54875, Republic of Korea.
authors.forename: Mihwa
authors.initials: M
authors.lastname: Oh
authors.affiliation: Department of Food and Nutrition, College of Biotechnology and Agriculture Resource, Chung-Ang University, Seodongdaero, Daeduck, Anseong, Gyeonggi, 17546, Republic of Korea. cchoi@cau.ac.kr.
authors.forename: Changsun
authors.identifier: 0000-0001-7730-8538
authors.initials: C
authors.lastname: Choi
mesh_terms: D017103:Bacillus Phages / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D001409:Bacillus cereus / Q000821:virology*; D005502:Food / Q000821:virology*; D058507:Host Specificity; D017906:Siphoviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D012988:Soil Microbiology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D014764:Viral Proteins
keywords: Bacillus cereus; Bacteriophages; Characterization; Inhibition; Isolation; Virulence factor