title: Role of PIN1 on in vivo periodontal tissue and in vitro cells.
abstract: BACKGROUND
  Although expression of peptidyl-prolyl cis/trans isomerase NIMA-interacting 1 (PIN1) was reported in bone tissue, the precise role of PIN1 in periodontal tissue and cells remain unclear.
  
  
  METHODS
  To elucidate the roles of PIN1 in periodontal tissue, its expression in periodontal tissue and cells, and effects on in vitro 4 osteoblast differentiation and the underlying signaling mechanisms were evaluated.
  
  
  RESULTS
  PIN1 was expressed in mouse periodontal tissues including periodontal ligament cells (PDLCs), cementoblasts and osteoblasts at the developing root formation stage (postnatal, PN14) and functional stage of tooth (PN28). Treatment of PIN1 inhibitor juglone, and gene silencing by RNA interference promoted osteoblast differentiation in PDLCs and cementoblasts, whereas the overexpression of PIN1 inhibited. Moreover, osteogenic medium-induced activation of AMPK, mTOR, Akt, ERK, p38 and NF-jB pathways were enhanced by PIN1 siRNA, but attenuated by PIN1 overexpression. Runx2 expressions were induced by PIN1 siRNA, but downregulated by PIN1 overexpression.
  
  
  CONCLUSIONS
  In summary, this study is the first to demonstrate that PIN1 is expressed in developing periodontal tissue, and in vitro PDLCs and cementoblasts. PIN1 inhibition stimulates osteoblast differentiation, and thus may play an important role in periodontal regeneration.
authors.affiliation: Department of Orthodontics, School of Dentistry, Kyung Hee University, Seoul, Korea.
authors.forename: K-H
authors.initials: KH
authors.lastname: Park
authors.affiliation: Department of Orthodontics, School of Dentistry, Kyung Hee University, Seoul, Korea.
authors.forename: E-H
authors.initials: EH
authors.lastname: Cho
authors.affiliation: Department of Oral and Maxillofacial Pathology, and Research Center for Tooth and Periodontal Regeneration (MRC), School of Dentistry, Kyung Hee University, Seoul, Korea.
authors.forename: W-J
authors.initials: WJ
authors.lastname: Bae
authors.affiliation: Department of Dentistry, Graduate School, Kyung Hee University, Seoul, Korea.
authors.forename: H-S
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Periodontology, School of Dentistry, Kyung Hee University, Seoul, Korea.
authors.forename: H-C
authors.initials: HC
authors.lastname: Lim
authors.affiliation: Department of Preventive and Society Dentistry, School of Dentistry, Kyung Hee University, Seoul, Korea.
authors.forename: Y-D
authors.initials: YD
authors.lastname: Park
authors.affiliation: Department of Dentistry, Graduate School, Kyung Hee University, Seoul, Korea.
authors.forename: M-O
authors.initials: MO
authors.lastname: Lee
authors.affiliation: Department of Oral Anatomy, Cluster for Craniofacial Development and Regeneration Research, Institute of Oral Biosciences, School of Dentistry, Chonbuk National University, Jeonju, Korea.
authors.forename: E-S
authors.initials: ES
authors.lastname: Cho
authors.affiliation: Department of Oral and Maxillofacial Pathology, and Research Center for Tooth and Periodontal Regeneration (MRC), School of Dentistry, Kyung Hee University, Seoul, Korea.
authors.forename: E-C
authors.initials: EC
authors.lastname: Kim
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D003739:Dental Cementum / Q000378:metabolism; D066298:In Vitro Techniques; D051379:Mice; D000072340:NIMA-Interacting Peptidylprolyl Isomerase / Q000378:metabolism / Q000502:physiology*; D010006:Osteoblasts / Q000378:metabolism; D010513:Periodontal Ligament / Q000378:metabolism; D010519:Periodontium / Q000166:cytology / Q000378:metabolism*
chemical_list: D000072340:NIMA-Interacting Peptidylprolyl Isomerase; C000606850:Pin1 protein, mouse
keywords: PIN1; cementoblasts; developing periodontium; differentiation; periodontal ligament cells