title: Assessing the iron chelation capacity of goat casein digest isolates.
abstract: We isolated goat phosphopeptides via calcium and ethanol precipitation from a caseinate digest and investigated their feasibility as an iron-fortification ingredient in nutritional foods. Goat tryptic-digested phosphopeptides could bind 54.37 ± 0.50 mg of Fe/g of protein compared with goat milk, which could bind 3.83 ± 0.01 mg of Fe/g of protein, indicating that isolation did increase iron binding. However, the >13-fold increase in iron binding was only partly explained by the increased concentration of phosphoserine-rich residues in the isolated fraction: we observed a 77% increase in serine residue content and a 5.9-fold increase in phosphorus in the goat peptide isolate compared with the starting caseinate material. We investigated the effect of potential industrial processing conditions (including heating, cooling, holding time, and processing order) on iron binding by the tryptic-digested phosphopeptides. In addition, we tested the effect of ionic strength and the addition of peptides to a milk system to understand how food formulations could affect iron binding.
authors.affiliation: Massey Institute of Food Science and Technology, School of Food and Nutrition, Massey University, Palmerston North, 4442, New Zealand.
authors.forename: A
authors.initials: A
authors.lastname: Smialowska
authors.affiliation: Massey Institute of Food Science and Technology, School of Food and Nutrition, Massey University, Palmerston North, 4442, New Zealand.
authors.forename: L
authors.initials: L
authors.lastname: Matia-Merino
authors.affiliation: Massey Institute of Food Science and Technology, School of Food and Nutrition, Massey University, Palmerston North, 4442, New Zealand. Electronic address: A.J.Carr@massey.ac.nz.
authors.forename: A J
authors.initials: AJ
authors.lastname: Carr
mesh_terms: D000818:Animals; D002364:Caseins; D006041:Goats / Q000378:metabolism*; D007501:Iron; D007502:Iron Chelating Agents; D008892:Milk / Q000737:chemistry; D010748:Phosphopeptides
chemical_list: D002364:Caseins; D007502:Iron Chelating Agents; D010748:Phosphopeptides; D007501:Iron
keywords: chelation; goat casein; iron; peptides