title: Multicolor Gold-Silver Nano-Mushrooms as Ready-to-Use SERS Probes for Ultrasensitive and Multiplex DNA/miRNA Detection.
abstract: Uniform silver-containing metal nanostructures with strong and stable surface-enhanced Raman scattering (SERS) signals hold great promise for developing ultrasensitive probes for biodetection. Nevertheless, the direct synthesis of such ready-to-use nanoprobes remains extremely challenging. Herein we report a DNA-mediated gold-silver nanomushroom with interior nanogaps directly synthesized and used for multiplex and simultaneous SERS detection of various DNA and RNA targets. The DNA involved in the nanostructures can act as not only gap DNA (mediated DNA) but also probe DNA (hybridized DNA), and DNA's involvement enables the nanostructures to have the inherent ability to recognize DNA and RNA targets. Importantly, we were the first to establish a new method for the generation of multicolor SERS probes using two different strategies. First Raman-labeled alkanethiol probe DNA was assembled on gold nanoparticles, and second, thiol-containing Raman reporters were coassembled with the probe DNA. The ready-to-use probes also give great potential to develop ultrasensitive detection methods for various biological molecules.
authors.affiliation: Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Su
authors.affiliation: Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, China.
authors.forename: Dongfang
authors.initials: D
authors.lastname: Wang
authors.affiliation: Institute of Inorganic Chemistry, University of Cologne , Cologne D-50939, Germany.
authors.forename: Lena
authors.initials: L
authors.lastname: Nörbel
authors.affiliation: Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, China.
authors.forename: Jianlei
authors.initials: J
authors.lastname: Shen
authors.affiliation: Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, China.
authors.forename: Zhihan
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, China.
authors.forename: Yanzhi
authors.initials: Y
authors.lastname: Dou
authors.affiliation: Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, China.
authors.forename: Tianhuan
authors.initials: T
authors.lastname: Peng
authors.affiliation: Kellogg College, University of Oxford , Oxford OX2 6PN, United Kingdom.
authors.forename: Jiye
authors.initials: J
authors.lastname: Shi
authors.affiliation: Institute of Inorganic Chemistry, University of Cologne , Cologne D-50939, Germany.
authors.forename: Sanjay
authors.identifier: 0000-0003-2765-2693
authors.initials: S
authors.lastname: Mathur
authors.affiliation: Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, China.
authors.forename: Chunhai
authors.identifier: 0000-0002-7171-7338
authors.initials: C
authors.lastname: Fan
authors.affiliation: Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, China.
authors.forename: Shiping
authors.identifier: 0000-0002-0791-8012
authors.initials: S
authors.lastname: Song
mesh_terms: D004247:DNA / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis; D006046:Gold / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D035683:MicroRNAs / Q000032:analysis* / Q000097:blood; D049329:Nanostructures / Q000737:chemistry*; D012367:RNA, Viral / Q000032:analysis; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D004279:DNA, Viral; D035683:MicroRNAs; D012367:RNA, Viral; D013438:Sulfhydryl Compounds; D012834:Silver; D006046:Gold; D004247:DNA