title: Decreased REG1α expression suppresses growth, invasion and angiogenesis of bladder cancer.
abstract: BACKGROUND
  Previous study has indicated association between REG1α and bladder cancer. The aim of this study was to investigate the role of Regenerating gene I alpha (REG1α) in bladder cancer.
  
  
  METHODS
  The role of REG1α in bladder cancer cell proliferation, migration and VEGF-induced angiogenesis was explored in vitro and in vivo. Immunohistochemistry (IHC) analysis was assessed to determine the expression of REG1α in ten paired bladder cancer and adjacent non-cancerous tissues, and in 296 bladder cancer samples.
  
  
  RESULTS
  Down-regulation of REG1α expression significantly reduced the proliferation, migration, invasion and VEGF-induced angiogenesis in vitro and the growth of xenograft tumors in vivo. VEGF expression in bladder cancer is associated with REG1α expression and recurrence. REG1α was overexpressed in bladder cancer tissues compared with adjacent normal samples. Patients with elevated REG1α exhibited shorter recurrence times and poor survival.
  
  
  CONCLUSIONS
  Downregulation of REG1α expression can reduce tumor growth, migration, invasion and angiogenesis. Our study demonstrates that REG1α can be used as a marker of recurrence and prognosis in bladder cancer. Therefore, REG1α targeting in bladder cancer patients represents a promising therapeutic strategy.
authors.affiliation: Department of Urology, Tenth People's Hospital, Tongji University, Shanghai, 200072, China. Electronic address: gengjiangsn@sina.com.
authors.forename: J
authors.initials: J
authors.lastname: Geng
authors.affiliation: Department of Pathology, Huashan Hospital, Fudan University, Shanghai, 200040, China; Department of Pathology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
authors.forename: J
authors.initials: J
authors.lastname: Fan
authors.affiliation: Department of Urology, Tenth People's Hospital, Tongji University, Shanghai, 200072, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Urology, Tenth People's Hospital, Tongji University, Shanghai, 200072, China.
authors.forename: X-P
authors.initials: XP
authors.lastname: Zhang
authors.affiliation: Department of Urology, Tenth People's Hospital, Tongji University, Shanghai, 200072, China.
authors.forename: L
authors.initials: L
authors.lastname: Kang
authors.affiliation: Department of Pathology, Tenth People's Hospital, Tongji University, Shanghai, 200072, China.
authors.forename: Q-Y
authors.initials: QY
authors.lastname: Li
authors.affiliation: Department of Urology, Tenth People's Hospital, Tongji University, Shanghai, 200072, China.
authors.forename: Y-F
authors.initials: YF
authors.lastname: Xu
authors.affiliation: Department of Urology, Tenth People's Hospital, Tongji University, Shanghai, 200072, China.
authors.forename: B
authors.initials: B
authors.lastname: Peng
authors.affiliation: Department of Urology, Tenth People's Hospital, Tongji University, Shanghai, 200072, China.
authors.forename: J-H
authors.initials: JH
authors.lastname: Zheng
authors.affiliation: Department of Urology, Tenth People's Hospital, Tongji University, Shanghai, 200072, China. Electronic address: yaoxudong67@sina.com.
authors.forename: X-D
authors.initials: XD
authors.lastname: Yao
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002295:Carcinoma, Transitional Cell / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D051847:Lithostathine / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D034622:RNA Interference; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D051847:Lithostathine; C497617:REG1A protein, human; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A
keywords: Angiogenesis; Bladder cancer; REG1α; Urothelial carcinoma; VEGF