title: Complete genome sequence of bean leaf crumple virus, a novel begomovirus infecting common bean in Colombia.
abstract: A copy of the complete genome of a novel bipartite begomovirus infecting common bean (Phaseolus vulgaris L.) in Colombia was obtained by rolling-circle amplification (RCA), cloned, and sequenced. The virus is associated with leaf crumple symptoms and significant yield losses in Andean and Mesoamerican beans. Such symptoms have been reported increasingly in Colombia since at least 2002, and we detected the virus in leaf material collected since 2008. Sequence analysis showed that the virus is a member of a distinct species, sharing 81% and 76% nucleotide (nt) sequence identity (in DNA-A and DNA-B, respectively) to other begomoviruses infecting common bean in the Americas. The data obtained support the taxonomic status of this virus (putatively named 'bean leaf crumple virus', BLCrV) as a member of a novel species in the genus Begomovirus.
authors.affiliation: Virology Laboratory, Agrobiodiversity Research Area (AgBio), International Center for Tropical Agriculture (CIAT), Cali, Colombia.
authors.forename: Monica
authors.initials: M
authors.lastname: Carvajal-Yepes
authors.affiliation: Virology Laboratory, Agrobiodiversity Research Area (AgBio), International Center for Tropical Agriculture (CIAT), Cali, Colombia.
authors.forename: Leidy
authors.initials: L
authors.lastname: Zambrano
authors.affiliation: Bean Genetics Laboratory and CGIAR Research Program on Legumes, CIAT, Cali, Colombia.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Bueno
authors.affiliation: Bean Genetics Laboratory and CGIAR Research Program on Legumes, CIAT, Cali, Colombia.
authors.forename: Bodo
authors.initials: B
authors.lastname: Raatz
authors.affiliation: Virology Laboratory, Agrobiodiversity Research Area (AgBio), International Center for Tropical Agriculture (CIAT), Cali, Colombia. w.cuellar@cgiar.org.
authors.forename: Wilmer J
authors.initials: WJ
authors.lastname: Cuellar
mesh_terms: D001483:Base Sequence; D053839:Begomovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D003105:Colombia; D016679:Genome, Viral; D008969:Molecular Sequence Data; D027805:Phaseolus / Q000821:virology*; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D012367:RNA, Viral / Q000235:genetics
chemical_list: D012367:RNA, Viral