title: Neuroprotective property of low molecular weight fraction from B. jararaca snake venom in H2O2-induced cytotoxicity in cultured hippocampal cells.
abstract: In central nervous system cells, low molecular weight fractions (LMWF) from snake venoms can inhibit changes in mitochondrial membrane permeability, preventing the diffusion of cytochrome c to the cytoplasm, inhibiting the activation of pro-apoptotic factors. Here, we evaluated the neuroprotective activity of LMWF from Bothrops jararaca (Bj) snake venom in H2O2-induced cytotoxicity in cultured hippocampal cells. SDS-PAGE, FT-IR and MALDI-TOF analysis of LMWF (<14 kDa) confirmed the absence of high-molecular-weight proteins in the fraction. LMWF did not present cytotoxicity in all concentrations and time tested by MTT assay. Neuroprotection was evaluated in cells pretreated with LMWF for 4 h prior to the addition of 50 μM H2O2for 20 h. We demonstrated that LMWF reduced the argininosuccinate synthase (AsS) and superoxide dismutase (SOD1) expressions, suggesting that this fraction as an effective neuroprotective compound that could increase the hippocampal cells viability by attenuation of oxidative stress. In addition, LMWF protects against apoptosis induced by H2O2, reducing the expression of caspase-3 and caspase-8. Overall, this study opens new perspectives for the identification of new molecules for the development of drugs applied to the treatment of neurodegenerative diseases.
authors.affiliation: Natural and Humanities Sciences Center, Experimental Morphophysiology Laboratory Federal University of ABC (UFABC), Rua Arcturus, n° 03, Bloco Delta, São Bernardo do Campo, 09606-070, SP, Brazil.
authors.forename: Samyr Machado
authors.initials: SM
authors.lastname: Querobino
authors.affiliation: Natural and Humanities Sciences Center, Experimental Morphophysiology Laboratory Federal University of ABC (UFABC), Rua Arcturus, n° 03, Bloco Delta, São Bernardo do Campo, 09606-070, SP, Brazil.
authors.forename: Daniel Carneiro
authors.initials: DC
authors.lastname: Carrettiero
authors.affiliation: Instituto de Pesquisa e Desenvolvimento - IP&D, Universidade do Vale do Paraíba-UNIVAP, Av. Shishima Hifumi 2911, São José dos Campos, Urbanova, 12244-000, SP, Brazil.
authors.forename: Maricilia Silva
authors.initials: MS
authors.lastname: Costa
authors.affiliation: Natural and Humanities Sciences Center, Experimental Morphophysiology Laboratory Federal University of ABC (UFABC), Rua Arcturus, n° 03, Bloco Delta, São Bernardo do Campo, 09606-070, SP, Brazil. Electronic address: carlos.asilva@ufabc.edu.br.
authors.forename: Carlos
authors.initials: C
authors.lastname: Alberto-Silva
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001124:Argininosuccinate Synthase / Q000235:genetics / Q000378:metabolism; D017837:Bothrops; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D053181:Caspase 8 / Q000235:genetics / Q000378:metabolism; D053453:Caspase 9 / Q000235:genetics / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000633:toxicity*; D008970:Molecular Weight; D018696:Neuroprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D061251:Primary Cell Culture; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017208:Rats, Wistar; D012910:Snake Venoms / Q000737:chemistry / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism
chemical_list: D018696:Neuroprotective Agents; D012333:RNA, Messenger; D012910:Snake Venoms; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9; D001124:Argininosuccinate Synthase
keywords: B. jararaca; Low molecular weight fraction; Neurodegenerative diseases; Neuroprotective; Snake venom