title: Association between C677T polymorphism of MTHFR gene and risk of amyotrophic lateral sclerosis: Polish population study and a meta-analysis.
abstract: OBJECTIVE
  Genetic factors play a role in pathogenesis of amyotrophic lateral sclerosis (ALS). A few studies demonstrated that the TT genotype of C677T polymorphism of the 5,10-methylenetetrahydrofolate reductase (MTHFR) gene can increase the risk of sporadic ALS. The aim of our study was to determine the relationship between C677T polymorphism of MTHFR gene and the risk of sporadic ALS in Polish population and to perform the meta-analysis assessing the significance this polymorphism for the risk of ALS in Caucasian population.
  
  
  METHODS
  We included 251 patients with ALS and 500 control subjects recruited from Polish population and performed the meta-analysis of published data from Caucasian population. MTHFR C677T polymorphism was genotyped using a TaqMan assay and 7900HT Fast real Time PCR System.
  
  
  RESULTS
  The frequency of genotypes did not differ significantly between Polish ALS patients and control subjects (CC: 45.0 vs 45.8%, CT: 48.2 vs 45.0%, TT: 6.8 vs 9.2%, P=0.46). The meta-analysis including 863 ALS patients and 1362 controls revealed that TT genotype increases the risk of sporadic ALS in Caucasian population.
  
  
  CONCLUSIONS
  Although we did not find the association between C677T polymorphism of MTHRF gene and risk of ALS in Polish population, the results of meta-analysis suggest that the TT genotype can be a genetic risk factor for ALS in Caucasian population.
authors.affiliation: Department of Neurology, Jagiellonian University Medical College, Kraków, Poland. Electronic address: kamilaanna@op.pl.
authors.forename: Kamila
authors.initials: K
authors.lastname: Żur-Wyrozumska
authors.affiliation: Department of Neurology, Jagiellonian University Medical College, Kraków, Poland. Electronic address: pera@su.krakow.pl.
authors.forename: Joanna
authors.initials: J
authors.lastname: Pera
authors.affiliation: Department of Neurology, Jagiellonian University Medical College, Kraków, Poland. Electronic address: andziubek@wp.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Dziubek
authors.affiliation: Department of Neurology, Jagiellonian University Medical College, Kraków, Poland. Electronic address: masado@interia.pl.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Sado
authors.affiliation: Department of Neurology, Jagiellonian University Medical College, Kraków, Poland. Electronic address: agolenia@gmail.com.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Golenia
authors.affiliation: Department of Neurology, Jagiellonian University Medical College, Kraków, Poland. Electronic address: agnieszka.slowik@uj.edu.pl.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Słowik
authors.affiliation: Department of Neurology, Jagiellonian University Medical College, Kraków, Poland. Electronic address: tomaszj.dziedzic@uj.edu.pl.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Dziedzic
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D000690:Amyotrophic Lateral Sclerosis / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D011044:Poland; D011110:Polymorphism, Genetic / Q000235:genetics*; D012306:Risk
chemical_list: C573423:MTHFR protein, human; D042965:Methylenetetrahydrofolate Reductase (NADPH2)
keywords: 5,10-Methylenetetrahydrofolate reductase; Amyotrophic lateral sclerosis; Genetics; Polymorphism