title: Reassessment of the taxonomic position of Burkholderia andropogonis and description of Robbsia andropogonis gen. nov., comb. nov.
abstract: The phylogenetic classification of the species Burkholderia andropogonis within the Burkholderia genus was reassessed using 16S rRNA gene phylogenetic analysis and multilocus sequence analysis (MLSA). Both phylogenetic trees revealed two main groups, named A and B, strongly supported by high bootstrap values (100%). Group A encompassed all of the Burkholderia species complex, whi.le Group B only comprised B. andropogonis species, with low percentage similarities with other species of the genus, from 92 to 95% for 16S rRNA gene sequences and 83% for conserved gene sequences. Average nucleotide identity (ANI), tetranucleotide signature frequency, and percentage of conserved proteins POCP analyses were also carried out, and in the three analyses B. andropogonis showed lower values when compared to the other Burkholderia species complex, near 71% for ANI, from 0.484 to 0.724 for tetranucleotide signature frequency, and around 50% for POCP, reinforcing the distance observed in the phylogenetic analyses. Our findings provide an important insight into the taxonomy of B. andropogonis. It is clear from the results that this bacterial species exhibits genotypic differences and represents a new genus described herein as Robbsia andropogonis gen. nov., comb. nov.
authors.affiliation: Laboratório de Bacteriologia Vegetal, Instituto Biológico, Rod. Heitor Penteado KM 3, Campinas, SP, CEP 13092-543, Brazil.
authors.forename: Lucilene
authors.initials: L
authors.lastname: Lopes-Santos
authors.affiliation: Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas - UNICAMP, Campinas, Brazil.
authors.forename: Daniel Bedo Assumpção
authors.initials: DBA
authors.lastname: Castro
authors.affiliation: Laboratório de Bacteriologia Vegetal, Instituto Biológico, Rod. Heitor Penteado KM 3, Campinas, SP, CEP 13092-543, Brazil.
authors.forename: Mariana
authors.initials: M
authors.lastname: Ferreira-Tonin
authors.affiliation: Laboratório de Bacteriologia Vegetal, Instituto Biológico, Rod. Heitor Penteado KM 3, Campinas, SP, CEP 13092-543, Brazil.
authors.forename: Daniele Bussioli Alves
authors.initials: DBA
authors.lastname: Corrêa
authors.affiliation: Landcare Research, Private Bag 92170, Auckland, New Zealand.
authors.forename: Bevan Simon
authors.initials: BS
authors.lastname: Weir
authors.affiliation: Landcare Research, Private Bag 92170, Auckland, New Zealand.
authors.forename: Duckchul
authors.initials: D
authors.lastname: Park
authors.affiliation: Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas - UNICAMP, Campinas, Brazil.
authors.forename: Laura Maria Mariscal
authors.initials: LMM
authors.lastname: Ottoboni
authors.affiliation: Laboratório de Bacteriologia Vegetal, Instituto Biológico, Rod. Heitor Penteado KM 3, Campinas, SP, CEP 13092-543, Brazil.
authors.forename: Júlio Rodrigues
authors.initials: JR
authors.lastname: Neto
authors.affiliation: Laboratório de Bacteriologia Vegetal, Instituto Biológico, Rod. Heitor Penteado KM 3, Campinas, SP, CEP 13092-543, Brazil. suzete@biologico.sp.gov.br.
authors.forename: Suzete Aparecida Lanza
authors.initials: SAL
authors.lastname: Destéfano
mesh_terms: D015373:Bacterial Typing Techniques; D019117:Burkholderia / Q000145:classification / Q000235:genetics; D002965:Classification; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005838:Genotype; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA gene; Bacterial disease; Genome sequence; MLSA