title: RNA interference influenced the proliferation and invasion of XWLC-05 lung cancer cells through inhibiting aquaporin 3.
abstract: The objective of this study was to construct a recombinant vector expressing siRNA targetedly inhibiting aquaporin 3 (AQP3), and to evaluate the effects of AQP3 inhibition on the proliferation and invasion of XWLC-05 human lung cancer cells.
  
  
  
  We obtained human AQP3 sequence from the Genbank and established the recombinant vector expressing siRNA targeting AQP3. After the transfection of the recombinant vectors, the expression of AQP3 was determined by RT-PCR and western blot. The MTS assay, flow cytometry and Transwell assay were conducted to detect the proliferation, cell cycle process, apoptosis and invasion of XWLC-05 cells. Then the activity of metal matrix proteinase (MMP) 2 was determined by gelatin zymography. Tumor formation in vivo experiments were also conducted in nude mice.
  
  
  
  RNA interference (RNAi) of AQP3 substantially suppressed the XWLC-05 cell proliferation and invasion, blocked the cell cycle progressing and promoted cell apoptosis. In addition, the activity of MMP2 was remarkably attenuated in RNAi group. AQP3 RNAi did not affect the tumor formation rate in nude mice but reduced the tumor growth.
  
  
  
  The inhibition of AQP3 retarded the growth and invasiveness of XWLC-05 lung cancer cells and decreased the activity of MMP2.
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China.
authors.forename: Guosheng
authors.initials: G
authors.lastname: Xiong
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Department of Oncology, General Hospital of Armed Police Forces of Yunnan Province, Kunming 650224, Yunnan, China.
authors.forename: Wanling
authors.initials: W
authors.lastname: Chen
authors.affiliation: Editorial Department, Kunming Medical University, Kunming 650500, Yunnan, China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China. Electronic address: yiquansu@126.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D051400:Aquaporin 3 / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D002453:Cell Cycle / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D000067492:RNAi Therapeutics / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction; D047368:Tumor Burden / Q000235:genetics; D023041:Xenograft Model Antitumor Assays / Q000379:methods
chemical_list: D051400:Aquaporin 3; D020778:Matrix Metalloproteinase 2
keywords: Aquaporin 3; Invasion; Lung cancer; Proliferation; RNA interference