title: Biotic and Abiotic Stresses Activate Different Ca2+Permeable Channels inArabidopsis.
abstract: To survive, plants must respond rapidly and effectively to various stress factors, including biotic and abiotic stresses. Salinity stress triggers the increase of cytosolic free Ca2+concentration ([Ca2+]i) via Ca2+influx across the plasma membrane, as well as bacterial flg22 and plant endogenous peptide Pep1. However, the interaction between abiotic stress-induced [Ca2+]iincreases and biotic stress-induced [Ca2+]iincreases is still not clear. Employing an aequorin-based Ca2+imaging assay, in this work, we investigated the [Ca2+]ichanges in response to flg22, Pep1, and NaCl treatments inArabidopsis thaliana. We observed an additive effect on the [Ca2+]iincrease which induced by flg22, Pep1, and NaCl. Our results indicate that biotic and abiotic stresses may activate different Ca2+permeable channels. Further, calcium signal induced by biotic and abiotic stresses was independent in terms of spatial and temporal patterning.
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University Hangzhou, China.
authors.forename: Xiao-Qiang
authors.initials: XQ
authors.lastname: Cao
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal UniversityHangzhou, China; Department of Biology, Duke University, DurhamNC, USA.
authors.forename: Zhong-Hao
authors.initials: ZH
authors.lastname: Jiang
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University Hangzhou, China.
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Yi
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University Hangzhou, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences, Zhejiang Sci-Tech University Hangzhou, China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Ke
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal UniversityHangzhou, China; Department of Biology, Duke University, DurhamNC, USA.
authors.forename: Zhen-Ming
authors.initials: ZM
authors.lastname: Pei
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University Hangzhou, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Zhu
keywords: Pep1; abiotic stress; aequorin-based Ca2+ imaging; biotic stress; calcium signal; flg22