title: Establishment and characterization of a new human acinar cell carcinoma cell line, Faraz-ICR, from pancreas.
abstract: OBJECTIVE
  Basic research in the field of acinar cell carcinoma (ACC) as a rare neoplasm of the pancreas is dependent on the availability of pragmatic model such as new pancreatic cancer cell lines. Thus, establishment and characterization of new pancreatic cancer cell lines from ACC origin are deemed important.
  
  
  METHODS
  Faraz-ICR cell line was derived from a 58-years old woman with pancreatic acinar cell carcinoma by the collagenase digestion protocol. We characterized the cell line by examining its morphology and cytostructural and functional profile.
  
  
  RESULTS
  Faraz-ICR has a doubling time of 35 hours and grows in soft agar with a colony-forming efficiency of 25%. The cell had nearly normal pattern of chromosomes in karyotype analysis and Comparative Genomic Hybridization (CGH) array analysis. Evaluation of cells by flowcytometry showed that Faraz-ICR is negative for EpCAM and mesenchymal markers in different passages, and has epithelial nature. Immunofluorescence staining revealed that cells were strongly positive for vimentin, desmin, ezrin, S100, nestin and they were negative for pan-cytokeratins, chromogranin and alpha smooth muscle actin.
  
  
  CONCLUSIONS
  We were able to establish a new pancreatic carcinoma cell line with partial aspects of Epithelial-mesenchymal transition and aggressiveness. This cell line might be suitable for studying various anticancer drugs and protein profile aiming to see any possible tumor associated marker for ACC.
authors.affiliation: Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Marzieh
authors.initials: M
authors.lastname: Rezaei
authors.affiliation: Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Hosseini
authors.affiliation: Department of Surgery, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Saman
authors.initials: S
authors.lastname: Nikeghbalian
authors.affiliation: Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address: ghaderia@sums.ac.ir.
authors.forename: Abbas
authors.initials: A
authors.lastname: Ghaderi
mesh_terms: D000782:Aneuploidy; D018267:Carcinoma, Acinar Cell; D045744:Cell Line, Tumor; D054443:Cell Migration Assays; D049109:Cell Proliferation; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008875:Middle Aged; D010190:Pancreatic Neoplasms
keywords: Acinar cell carcinoma; Cell line; Pancreatic cancer