title: Angiotensin II Type 1 Receptor Gene A1166C Polymorphism Was Not Associated With Acute Coronary Syndrome in an Iranian Population.
abstract: BACKGROUND
  There are very limited data for Iranian populations on the predisposing genetic factors for acute coronary syndrome (ACS).
  
  
  OBJECTIVE
  The objective of the present study was to investigate the association of the angiotensin II type 1 receptor (AT1R) gene polymorphism and ACS in an Iranian population.
  
  
  METHODS
  This cross-sectional study was conducted among 263 subjects (97 men and 166 women). Patients (n = 128) aged 30 - 80 years with chest pain were recruited from the emergency department of Ghaem Hospital (Mashhad, Iran). A 12-lead electrocardiograph plus creatine kinase MB (CK-MB) levels were used as the basis for the diagnosis of myocardial ischemia. The control group was selected from age-matched healthy subjects (n = 135). Non-enzymatic kits were used for extraction of DNA from blood samples. Polymerase chain reaction (PCR) was performed to amplify the DNA fragments. For restriction fragment length polymorphism (RFLP) determination, the DdeI enzyme was used to digest the amplified DNA fragments. Statistical analyses were performed using SPSS version 13.0.
  
  
  RESULTS
  There was no statistical difference in the genotype frequency of patients and healthy subjects with regard to age and gender (P > 0.05).
  
  
  CONCLUSIONS
  The AT1R A1166C polymorphism appeared not to be associated with the presence of ACS in the population studied.
authors.affiliation: Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors.forename: Navid
authors.initials: N
authors.lastname: Delshad
authors.affiliation: Cardiovascular Research Center, Avicenna Research institute, Mashhad University of Medical Sciences, Mashhad, IR Iran; Biochemistry and Nutrition Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Ghayour-Mobarhan
authors.affiliation: Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors.forename: Hamed
authors.initials: H
authors.lastname: Mirzaei
authors.affiliation: Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors.forename: Kamal
authors.initials: K
authors.lastname: Razavi-Azarkhiavi
authors.affiliation: Department of Cardiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Moohebati
authors.affiliation: Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors.forename: Mitra
authors.initials: M
authors.lastname: Hassany
authors.affiliation: Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors.forename: Jamal
authors.initials: J
authors.lastname: Kasaian
authors.affiliation: Cardiovascular Research Center, Avicenna Research institute, Mashhad University of Medical Sciences, Mashhad, IR Iran; Department of Cardiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Etemadzadeh
authors.affiliation: Cardiovascular Research Center, Avicenna Research institute, Mashhad University of Medical Sciences, Mashhad, IR Iran; Department of Cardiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors.forename: Maryam Sadat
authors.initials: MS
authors.lastname: Alavi
authors.affiliation: Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, IR Iran; Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors.forename: Javad
authors.initials: J
authors.lastname: Behravan
keywords: AT1R A1166C; Acute Coronary Syndrome; Angiotensin II Type 1 Receptor; Gene Polymorphisms; Genotype Frequency