title: Inhibition of the Innate Immune Receptors for Foreign DNA Sensing Improves Transfection Efficiency of Gene Electrotransfer in Melanoma B16F10 Cells.
abstract: Gene electrotransfer upregulate DNA pattern recognition receptors or DNA sensors, which are part of the innate immune system. In this study, we tested if addition of the cocktail of innate immune system inhibitors to the cells during gene electrotransfer (GET) can increase transfection efficiency and cell survival. The results indicate that this cocktail can decrease cytosolic DNA sensors expression after GET, and consequently increase cell survival and transfection efficiency in B16 cells, but only in highly metastatic B16F10 subtype. We demonstrated that DNA sensors expression during the transfection methods needs to be downregulated if higher transfection efficiency and better cells' survival is needed. The inhibition of the receptors of the innate immune system can improve the transfection efficiency also for GET of malignant melanoma B16 cells, but only of highly metastatic subtype.
authors.affiliation: Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska 2, 1000, Ljubljana, Slovenia.
authors.forename: Masa
authors.initials: M
authors.lastname: Bosnjak
authors.affiliation: Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska 2, 1000, Ljubljana, Slovenia.
authors.forename: Urska
authors.initials: U
authors.lastname: Kamensek
authors.affiliation: Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska 2, 1000, Ljubljana, Slovenia.
authors.forename: Gregor
authors.initials: G
authors.lastname: Sersa
authors.affiliation: Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska 2, 1000, Ljubljana, Slovenia.
authors.forename: Danijela
authors.initials: D
authors.lastname: Stolfa
authors.affiliation: Department of Cytopathology, Institute of Oncology Ljubljana, Zaloska 2, 1000, Ljubljana, Slovenia.
authors.forename: Jaka
authors.initials: J
authors.lastname: Lavrencak
authors.affiliation: Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska 2, 1000, Ljubljana, Slovenia. mcemazar@onko-i.si.|Faculty of Health Sciences, University of Primorska, Polje 42, 6310, Izola, Slovenia. mcemazar@onko-i.si.
authors.forename: Maja
authors.initials: M
authors.lastname: Cemazar
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004247:DNA / Q000378:metabolism*; D018274:Electroporation / Q000379:methods*; D018014:Gene Transfer Techniques; D007113:Immunity, Innate / Q000502:physiology; D051379:Mice; D014162:Transfection / Q000379:methods*
chemical_list: D004247:DNA
keywords: DNA sensors; Gene electrotransfer; Innate immune system inhibitors