title: Disturbed mitochondrial function restricts glutamate uptake in the human Müller glia cell line, MIO-M1.
abstract: Using the human Müller cell line, MIO-M1, the aim was to study the impact of mitochondrial inhibition in Müller glia through antimycin A treatment. MIO-M1 cell survival, levels of released lactate, mitochondrial function, and glutamate uptake were studied in response to mitochondrial inhibition and glucose restriction. Lactate release decreased in response to glucose restriction. Combined glucose restriction and blocked mitochondrial activity decreased survival and caused collapse of the respiratory chain measured by oxygen consumption rate and extracellular acidification rate. Mitochondrial inhibition caused impaired glutamate uptake and decreased mRNA expression of the glutamate transporter, EAAT1. Over all, we show important roles of mitochondrial activity in MIO-M1 cell function and survival.
authors.affiliation: University of Copenhagen, Department of Neuroscience and Pharmacology, Blegdamsvej 3B, Building 33.4.83, Copenhagen N, Denmark; University of Copenhagen, Department of Drug Design and Pharmacology, Universitetsparken 2, Building 22.4.405, 2100 Copenhagen Ø, Denmark; Zealand University Hospital, Department of Ophthalmology, Vestermarksvej 23, 4000 Roskilde, Denmark.
authors.forename: Rupali
authors.initials: R
authors.lastname: Vohra
authors.affiliation: University of Copenhagen, Department of Neuroscience and Pharmacology, Blegdamsvej 3B, Building 33.4.83, Copenhagen N, Denmark.
authors.forename: Iswariyaraja Sridevi
authors.initials: IS
authors.lastname: Gurubaran
authors.affiliation: University of Copenhagen, Department of Neuroscience and Pharmacology, Blegdamsvej 3B, Building 33.4.83, Copenhagen N, Denmark.
authors.forename: Ulrik
authors.initials: U
authors.lastname: Henriksen
authors.affiliation: University of Copenhagen, Department of Neuroscience and Pharmacology, Blegdamsvej 3B, Building 33.4.83, Copenhagen N, Denmark; University of Copenhagen, Center for Healthy Aging, Blegdamsvej 3B, Building 18.1, 2200 Copenhagen N, Denmark; University of Oslo, Brain and Muscle Energy Group, Sognsvannsveien 10, 0316 Oslo, Norway.
authors.forename: Linda Hildegaard
authors.initials: LH
authors.lastname: Bergersen
authors.affiliation: University of Copenhagen, Center for Healthy Aging, Blegdamsvej 3B, Building 18.1, 2200 Copenhagen N, Denmark.
authors.forename: Lene Juel
authors.initials: LJ
authors.lastname: Rasmussen
authors.affiliation: Zealand University Hospital, Department of Ophthalmology, Vestermarksvej 23, 4000 Roskilde, Denmark.
authors.forename: Claus
authors.initials: C
authors.lastname: Desler
authors.affiliation: University of Copenhagen, Department of Neuroscience and Pharmacology, Blegdamsvej 3B, Building 33.4.83, Copenhagen N, Denmark; University of Copenhagen, Department of Drug Design and Pharmacology, Universitetsparken 2, Building 22.4.405, 2100 Copenhagen Ø, Denmark.
authors.forename: Dorte Marie
authors.initials: DM
authors.lastname: Skytt
authors.affiliation: University of Copenhagen, Department of Neuroscience and Pharmacology, Blegdamsvej 3B, Building 33.4.83, Copenhagen N, Denmark; University of Copenhagen, Department of Drug Design and Pharmacology, Universitetsparken 2, Building 22.4.405, 2100 Copenhagen Ø, Denmark; Zealand University Hospital, Department of Ophthalmology, Vestermarksvej 23, 4000 Roskilde, Denmark. Electronic address: mkolko@dadlnet.dk.
authors.forename: Miriam
authors.initials: M
authors.lastname: Kolko
mesh_terms: D000890:Anti-Infective Agents / Q000378:metabolism; D000968:Antimycin A / Q000378:metabolism; D001692:Biological Transport; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D063928:Ependymoglial Cells / Q000187:drug effects / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism*; D006801:Humans; D007773:Lactates / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000890:Anti-Infective Agents; D007773:Lactates; D018698:Glutamic Acid; D000968:Antimycin A; D005947:Glucose
keywords: Antimycin A; Glutamate; Lactate; MIO-M1 cell line; Mitochondria; Müller cells