title: Physiological Changes of Surface Membrane in Lactobacillus with Prebiotics.
abstract: Synbiotics are always considered to be beneficial in healthy manipulation of gut environment; however, the purpose of this research was to investigate the dominance of synbiotic over the individual potential of probiotics and prebiotics. Four different types of prebiotics, fructo-oligosaccharides, raffinose, inulin, and cellobiose, were evaluated based on their varying degree of polymerization, combined each with 2 different Lactobacilli strains, including Lactobacillus paracasei 276 and Lactobacillus plantarum WCFS1. The effects of synbiotics combination on the surface structure were evaluated by analyzing auto-aggregation, membrane hydrophobicity, and adhesion to Caco-2 cells. Our results showed that both Lactobacilli exhibited significantly greater degree of attachment to Caco-2 cells (23.31% and 16.85%, respectively) when using cellobiose as a substrate than with other prebiotics (P < 0.05). Intestinal adhesion ability was in correlation with the percent of auto-aggregation, both Lactobacillus exhibited higher percent of auto-aggregation in cellobiose compared to other prebiotics. These behavioral changes in terms of attachment and auto-aggregation were further supported with the changes noticed from infrared spectra (FT-IR).
authors.affiliation: Food and Nutritional Science, School of Biological Sciences, The Univ. of Hong Kong, Pokfulam Road, Hong Kong.
authors.forename: Mingfang
authors.initials: M
authors.lastname: Pan
authors.affiliation: Food and Nutritional Science, School of Biological Sciences, The Univ. of Hong Kong, Pokfulam Road, Hong Kong.
authors.forename: Kishore K
authors.initials: KK
authors.lastname: Kumaree
authors.affiliation: Food and Nutritional Science, School of Biological Sciences, The Univ. of Hong Kong, Pokfulam Road, Hong Kong.|Adjunct Professor, Victoria Univ., Melbourne, Australia.
authors.forename: Nagendra P
authors.initials: NP
authors.lastname: Shah
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects*; D018938:Caco-2 Cells; D002462:Cell Membrane / Q000187:drug effects* / Q000502:physiology; D002475:Cellobiose / Q000494:pharmacology; D005632:Fructose / Q000494:pharmacology; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007422:Intestines / Q000382:microbiology; D007444:Inulin / Q000494:pharmacology; D007778:Lactobacillus / Q000187:drug effects* / Q000254:growth & development; D009844:Oligosaccharides / Q000494:pharmacology*; D056692:Prebiotics; D019936:Probiotics; D011887:Raffinose / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D058616:Synbiotics
chemical_list: D009844:Oligosaccharides; D056692:Prebiotics; D002475:Cellobiose; D005632:Fructose; D007444:Inulin; D011887:Raffinose
keywords: Lactobacillus; membrane hydrophobicity; prebiotics; probiotics