title: Improvement of Polymyxin-Egg Yolk-Mannitol-Bromothymol Blue Agar for the Enumeration and Isolation of Bacillus cereus in Various Foods.
abstract: A modified polymyxin-egg yolk-mannitol-bromothymol blue agar (mPEMBA) was developed by supplementing polymyxin-egg yolk-mannitol-bromothymol blue agar (PEMBA) with trimethoprim to improve the selectivity for and recoverability of Bacillus cereus from naturally and artificially contaminated food samples. The number of B. cereus in mPEMBA was significantly higher than in PEMBA, indicating better recoverability (P < 0.05) in red pepper powder (PEMBA 0.80 ± 0.22 log CFU/g versus mPEMBA 1.95 ± 0.17 log CFU/g) and soybean paste (PEMBA 2.19 ± 0.18 log CFU/g versus mPEMBA 3.09 ± 0.13 log CFU/g). In addition, mPEMBA provided better visual differentiation of B. cereus colonies than PEMBA, which is attributable to the reduced number of competing microflora. We conclude that the addition of trimethoprim to PEMBA could generate a synergistic effect to improve selectivity for B. cereus .
authors.affiliation: Center for One Health, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of Korea.
authors.forename: Il-Byeong
authors.initials: IB
authors.lastname: Kang
authors.affiliation: Center for One Health, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of Korea.
authors.forename: Jung-Whan
authors.initials: JW
authors.lastname: Chon
authors.affiliation: Center for One Health, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of Korea.
authors.forename: Dong-Hyeon
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Center for One Health, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of Korea.
authors.forename: Dana
authors.initials: D
authors.lastname: Jeong
authors.affiliation: Center for One Health, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of Korea.
authors.forename: Hong-Seok
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Food & Nutrition, College of Human Ecology, Hanyang University, Seoul 04763, Republic of Korea.
authors.forename: Hyunsook
authors.initials: H
authors.lastname: Kim
authors.affiliation: Center for One Health, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of Korea.
authors.forename: Kun-Ho
authors.initials: KH
authors.lastname: Seo
mesh_terms: D000362:Agar; D001409:Bacillus cereus; D001979:Bromthymol Blue; D003470:Culture Media; D004530:Egg Yolk; D005516:Food Microbiology; D008353:Mannitol; D011113:Polymyxins
chemical_list: D003470:Culture Media; D011113:Polymyxins; D008353:Mannitol; D000362:Agar; D001979:Bromthymol Blue
keywords: Bacillus cereus; Competing microflora; Modified polymyxin–egg yolk–mannitol–bromothymol blue agar; Selective media; Trimethoprim