title: Differences in serum oxidative status between glaucomatous and nonglaucomatous cataract patients.
abstract: BACKGROUND
  Oxidative stress contributes to both intraocular pressure regulation and glaucomatous neuropathy. The systemic redox status (solitary determination) was examined in primary open-angle glaucoma (POAG) patients with cataract and nonglaucomatous cataract patients. Cataract-matched group comparisons appear more precise in the context of oxidative stress evaluation. The aim of this study was to establish if systemic oxidative status in POAG patients was elevated compared with the cataract only subjects.
  
  
  METHODS
  The study included patients with primary open angle glaucoma (POAG group, n = 30) and controls (non POAG group, n = 25). Serum concentration of lipofuscine (LPS), malondialdehyde (MDA) and activity of total superoxide dismutase (SOD), and its mitochondrial (Mn-SOD) and cystolic (Cu,Zn-SOD) isoform were measured. Total oxidant state (TOS) and total antioxidant capacity (TAC) in blood were also evaluated.
  
  
  RESULTS
  Significant increase of LPS (p = 0.0002) and MDA (p = 0.005) concentration was observed in glaucomatous patients as compared with controls. Total SOD activity was significantly lowered in the glaucoma group (p = 0.003); serum level of Mn-SOD was significantly lower in glaucoma patients (p = 0.048) however, Cu,Zn-SOD was not. Glaucoma patients presented elevated mean TOS (p = 0.016). Both groups presented with comparable TAC.
  
  
  CONCLUSIONS
  Systemic redox balance of cataract patients was significantly altered in the course of glaucoma.
authors.affiliation: Department and Clinic of Ophthalmology, School of Medicine in Katowice, Medical University of Silesia, Ceglana 35, 40-514, Katowice, Poland. wojtek.rokicki@gmail.com.
authors.forename: Wojciech
authors.identifier: http://orcid.org/0000-0001-9430-1506
authors.initials: W
authors.lastname: Rokicki
authors.affiliation: Department of Biochemistry, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia, Katowice, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Zalejska-Fiolka
authors.affiliation: Department and Clinic of Ophthalmology, School of Medicine in Katowice, Medical University of Silesia, Ceglana 35, 40-514, Katowice, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Pojda-Wilczek
authors.affiliation: Department of Biochemistry, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia, Katowice, Poland.
authors.forename: Alicja
authors.initials: A
authors.lastname: Hampel
authors.affiliation: Radiotherapy Department, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Majewski
authors.affiliation: Department and Clinic of Ophthalmology, School of Medicine in Katowice, Medical University of Silesia, Ceglana 35, 40-514, Katowice, Poland.
authors.forename: Serap
authors.initials: S
authors.lastname: Ogultekin
authors.affiliation: Department and Clinic of Ophthalmology, School of Medicine in Katowice, Medical University of Silesia, Ceglana 35, 40-514, Katowice, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Mrukwa-Kominek
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000097:blood; D002386:Cataract / Q000097:blood* / Q000150:complications; D005260:Female; D005902:Glaucoma, Open-Angle / Q000097:blood* / Q000150:complications; D006801:Humans; D008062:Lipofuscin / Q000097:blood*; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000097:blood*
chemical_list: D015415:Biomarkers; D008062:Lipofuscin; D008315:Malondialdehyde; D013482:Superoxide Dismutase
keywords: Glaucoma; Neurodegenerative disease; Oxidative stress; Serum