title: Development of a traceable molecular hygiene control method (TMHCM) for human DNA content in foods.
abstract: The aim of this study was to develop a molecular technique to determine the level of human originated DNA contamination in unhygienic food products. In the study, four model foods were prepared under both hygienic (H) and non-hygienic (NH) conditions and the human originated microbial loads of these products were determined. DNA was extracted from the model foods and human buccal samples by GIDAGEN Multi-fast DNA isolation kit. A primer specific region of human mitochondrial D-Loop was designed. The level of human DNA contamination in the model foods was determined by real-time PCR. The sensitivity of the technique developed here was 0.00001ng DNA/PCR. In addition, the applicability of the traceable molecular hygiene control method (TMHCM) was tested in 60 food samples from the market. The results of this study demonstrate that DNA based TMHCM can be used to predict to what extent foods meet the human oriented hygienic conditions.
authors.affiliation: Bioengineering Department, Çanakkale Onsekiz Mart University, Çanakkale, Turkey. Electronic address: ersakalar@gmail.com.
authors.forename: Ergün
authors.initials: E
authors.lastname: Şakalar
authors.affiliation: Bioengineering Department, Çanakkale Onsekiz Mart University, Çanakkale, Turkey.
authors.forename: Şeyma Özçirak
authors.initials: ŞÖ
authors.lastname: Ergün
authors.affiliation: Food Engineering Department, Çanakkale Onsekiz Mart University, Çanakkale, Turkey.
authors.forename: Çiğdem
authors.initials: Ç
authors.lastname: Pala
authors.affiliation: Bioengineering Department, Çanakkale Onsekiz Mart University, Çanakkale, Turkey.
authors.forename: Emine
authors.initials: E
authors.lastname: Akar
authors.affiliation: Bioengineering Department, Çanakkale Onsekiz Mart University, Çanakkale, Turkey.
authors.forename: Cengiz
authors.initials: C
authors.lastname: Ataşoğlu
mesh_terms: D004247:DNA / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology / Q000379:methods*; D059022:Food Safety / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004247:DNA
keywords: DNA; Food; Human contamination; Hygiene; Molecular methodology; Real-time PCR