title: Phytosphingosine-1-phosphate and epidermal growth factor synergistically restore extracellular matrix in human dermal fibroblasts in vitro and in vivo.
abstract: Phytosphingosine-1-phosphate (PhS1P), which is found in plants and fungi, is generated by the phosphorylation of phytosphingosine and is structurally similar to molecules that promote cellular growth and proliferation. The aim of this study was to ascertain whether PhS1P displays synergistic effects together with epidermal growth factor (EGF), which is also critical for activating proliferation, migration and survival pathways. We utilized cultured human dermal fibroblasts (HDFs) and a number of assays, including western blotting, cell migration assays, quantitative (real-time) PCR, and viability assays. We found that PhS1P promoted the activity of EGF in vitro. We then conducted a clinical trial in females over 35 years of age, with visible signs of skin aging. By evaluating skin hydration, dermal density and thickness, length of fine wrinkles, and skin elasticity, we verified the clinical efficacy of a combined treatment of PhS1P and EGF in vivo. On the whole, our data suggest that PhS1P displays a synergistic anti-aging effect together with EGF, both in vitro and in vivo.
authors.affiliation: KISCS Incorporated, Cheongju-si, Chungcheongbuk-do 361-951, Republic of Korea.
authors.forename: Seung Bin
authors.initials: SB
authors.lastname: Kwon
authors.affiliation: Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Sungkwan
authors.initials: S
authors.lastname: An
authors.affiliation: Department of Dermatology, Konkuk University School of Medicine, Seoul 143-701, Republic of Korea.
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: KISCS Incorporated, Cheongju-si, Chungcheongbuk-do 361-951, Republic of Korea.
authors.forename: Ka Ram
authors.initials: KR
authors.lastname: Kim
authors.affiliation: Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Young Min
authors.initials: YM
authors.lastname: Choi
authors.affiliation: Department of Dermatology, Konkuk University School of Medicine, Seoul 143-701, Republic of Korea.
authors.forename: Kyu Joong
authors.initials: KJ
authors.lastname: Ahn
authors.affiliation: KISCS Incorporated, Cheongju-si, Chungcheongbuk-do 361-951, Republic of Korea.
authors.forename: In-Sook
authors.initials: IS
authors.lastname: An
authors.affiliation: Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Hwa Jun
authors.initials: HJ
authors.lastname: Cha
mesh_terms: D002465:Cell Movement; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology* / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D014945:Wound Healing
chemical_list: D008024:Ligands; C117422:phytosphingosine-1-phosphate; D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases; D013110:Sphingosine