title: Nuclear localization of a putative Phytophthora sojae bZIP1 transcription factor is mediated by multiple targeting motifs.
abstract: Oomycetes are fungal-like eukaryotic microbes in the kingdom Stramenopila. We recently found that the oomycete plant pathogen Phytophthora sojae uses nuclear localization signals (NLSs) for translocation of proteins into the nucleus that differ from conventional well-characterized NLSs from mammals and yeast. Here, we have characterized in depth the NLSs of a P. sojae basic leucine zipper transcription factor, PsbZIP1. Nuclear localization of PsbZIP1 was determined by a central conserved region overlapping the DNA binding domain. Mutational analysis of this region identified four distinct elements that contributed multiplicatively to nuclear localization, but the conserved DNA binding residues were not required. Three of the elements showed autonomous NLS activity and the fourth served as a nuclear localization enhancer. Sequences within two of the nuclear localization elements defined a new form of bipartite NLS consisting of a triplet of basic residues followed by a tail of scattered basic amino acids.
authors.affiliation: Interdisciplinary Ph.D. Program in Genetics, Bioinformatics & Computational Biology, Virginia Polytechnic Institute and State University (Virginia Tech), Blacksburg, VA, 24061, USA.|Center for Genome Research and Biocomputing and Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, 97331, USA.
authors.forename: Yufeng
authors.identifier: 0000-0002-5440-2246
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Interdisciplinary Ph.D. Program in Genetics, Bioinformatics & Computational Biology, Virginia Polytechnic Institute and State University (Virginia Tech), Blacksburg, VA, 24061, USA.|Center for Genome Research and Biocomputing and Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, 97331, USA.
authors.forename: Brett M
authors.initials: BM
authors.lastname: Tyler
mesh_terms: D020816:Amino Acid Motifs / Q000235:genetics; D000595:Amino Acid Sequence / Q000235:genetics; D050976:Basic-Leucine Zipper Transcription Factors / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D008969:Molecular Sequence Data; D019913:Nuclear Localization Signals / Q000235:genetics* / Q000378:metabolism; D010838:Phytophthora / Q000235:genetics / Q000378:metabolism*; D000072417:Protein Domains / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; D019913:Nuclear Localization Signals; D014157:Transcription Factors