title: Ozone protects rat heart against ischemia-reperfusion injury: A role for oxidative preconditioning in attenuating mitochondrial injury.
abstract: Ischemia-reperfusion injury (IRI) is a major cause of cardiac dysfunction during cardiovascular surgery, heart transplantation and cardiopulmonary bypass procedures. The purpose of the present study was to explore, firstly, whether ozone induces oxidative preconditioning by activation of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and, secondly, whether ozone oxidative preconditioning (OzoneOP) can protect the heart against IRI by attenuating mitochondrial damage. Rats were subjected to 30min of cardiac ischemia followed by 2h of reperfusion, with or without prior OzoneOP (100μg/kg/day) for 5 days. Antioxidant capacity, myocardial apoptosis and mitochondrial damage were evaluated and compared at the end of reperfusion. OzoneOP was found to increase antioxidant capacity and to protect the myocardium against IRI by attenuating mitochondrial damage and myocardial apoptosis. The study suggests a potential role for OzoneOP in protecting the heart against IRI during cardiovascular surgery, cardiopulmonary bypass procedures or transplantation.
authors.affiliation: Department: Cardiovascular Surgery, Institution: The First Affiliated Hospital of Harbin Medical University, Address: 23 Youzheng Street, Harbin, Heilongjiang 150001, China.
authors.forename: Weixin
authors.initials: W
authors.lastname: Meng
authors.affiliation: Department: Editorial Board of Journal of Harbin Medical University, Institution: Harbin Medical University, Address: 157 Baojian Road, Harbin, Heilongjiang 150081, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department: Institute of Keshan Disease, Institution: Harbin Medical University, Address: 157 Baojian Road, Harbin, Heilongjiang 150081, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Li
authors.affiliation: Department: Cardiovascular Surgery, Institution: The First Affiliated Hospital of Harbin Medical University, Address: 23 Youzheng Street, Harbin, Heilongjiang 150001, China.
authors.forename: Erjun
authors.initials: E
authors.lastname: Zhu
authors.affiliation: Department: Cardiovascular Surgery, Institution: The First Affiliated Hospital of Harbin Medical University, Address: 23 Youzheng Street, Harbin, Heilongjiang 150001, China.
authors.forename: Li
authors.initials: L
authors.lastname: Deng
authors.affiliation: Department: Cardiovascular Surgery, Institution: The First Affiliated Hospital of Harbin Medical University, Address: 23 Youzheng Street, Harbin, Heilongjiang 150001, China.
authors.forename: Zonghong
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department: Cardiovascular Surgery, Institution: The First Affiliated Hospital of Harbin Medical University, Address: 23 Youzheng Street, Harbin, Heilongjiang 150001, China.
authors.forename: Guowei
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department: Cardiovascular Surgery, Institution: The First Affiliated Hospital of Harbin Medical University, Address: 23 Youzheng Street, Harbin, Heilongjiang 150001, China. Electronic address: liuhongyu_1963@126.com.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Liu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002316:Cardiotonic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D004562:Electrocardiography; D006352:Heart Ventricles / Q000187:drug effects / Q000473:pathology; D020287:In Situ Nick-End Labeling; D019194:Ischemic Preconditioning; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D008954:Models, Biological; D015428:Myocardial Reperfusion Injury / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000473:pathology; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction; D010126:Ozone / Q000494:pharmacology / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results
chemical_list: D000975:Antioxidants; D002316:Cardiotonic Agents; D051267:NF-E2-Related Factor 2; C495637:Nfe2l2 protein, rat; D012333:RNA, Messenger; D010126:Ozone
keywords: Heart; Ischemia-reperfusion injury; Mitochondrial injury; Ozone oxidative preconditioning