title: miR-296 inhibits the metastasis and epithelial-mesenchymal transition of colorectal cancer by targeting S100A4.
abstract: Dysregulation of microRNAs (miRNAs) is actively involved in the pathogenesis and tumorigenicity of colorectal cancer (CRC). miR-296 was found to play either oncogenic or tumor suppressive role in human cancers. However, the status of miR-296 and its function in CRC remain unknown.
  
  
  
  The expression of miR-296 was confirmed by qRT-PCR in CRC tissues and cells, and its level was altered by corresponding miRNA vectors. Wound healing and Transwall assays were performed to detect the migration and invasion of CRC cells. The levels of proteins were measured using immunoblotting, immunohistochemistry and immunofluorescence.
  
  
  
  Underexpression of miR-296 was disclosed in CRC tissues and cells. Its decreased level was evidently correlated with adverse clinical parameters and poor prognosis of CRC patients. In vitro experiments indicated that miR-296 inhibited CRC cell migration and invasion. Mechanically, miR-296 inhibited the epithelial-mesenchymal transition (EMT) of CRC cells. A negative correlation between miR-296 and S100A4 expression was observed in CRC tissues. Luciferase reporter assays indicated that miR-296 inversely regulated the luciferase activity of 3'-UTR of S100A4. Herein, S100A4 was found to be a downstream molecule of miR-296 in CRC. Furthermore, S100A4 mediated the anti-metastatic effects of miR-296 on EMT, migration and invasion of CRC cells.
  
  
  
  miR-296 functions as an anti-metastatic factor mainly by suppressing S100A4 in CRC. It potentially acts as a prognostic predictor and a drug-target for CRC patients.
authors.affiliation: Department of Clinical Laboratory, Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing, 100853, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: He
authors.affiliation: Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, 318000, China.
authors.forename: Lianhua
authors.initials: L
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Physical Chemistry of Solid Surfaces and College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.
authors.forename: Shiyi
authors.initials: S
authors.lastname: Luo
authors.affiliation: Beijing Center for Physical and Chemical Analysis, Beijing, 100094, China.
authors.forename: Mingzhen
authors.initials: M
authors.lastname: Li
authors.affiliation: Beijing Center for Physical and Chemical Analysis, Beijing, 100094, China.
authors.forename: Junbo
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory, Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing, 100853, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Clinical Laboratory, Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing, 100853, China. wcb_pla@163.com.
authors.forename: Chengbin
authors.initials: C
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D045744:Cell Line, Tumor; D003106:Colon / Q000737:chemistry / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D005260:Female; D055785:Gene Knockdown Techniques; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009362:Neoplasm Metastasis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D000071999:S100 Calcium-Binding Protein A4 / Q000032:analysis / Q000235:genetics* / Q000378:metabolism
chemical_list: C549871:MIRN296 microRNA, human; D035683:MicroRNAs; D000071999:S100 Calcium-Binding Protein A4; C077591:S100A4 protein, human
keywords: Colorectal cancer; Epithelial-mesenchymal transition; Metastasis; S100A4; miR-296