title: Induction of Ischemic Stroke and Ischemia-reperfusion in Mice Using the Middle Artery Occlusion Technique and Visualization of Infarct Area.
abstract: Cerebrovascular disease is highly prevalent in the global population and encompasses several types of conditions, including stroke. To study the impact of stroke on tissue injury and to evaluate the effectiveness of therapeutic interventions, several experimental models in a variety of species were developed. They include complete global cerebral ischemia, incomplete global ischemia, focal cerebral ischemia, and multifocal cerebral ischemia. The model described in this protocol is based on the middle cerebral artery occlusion (MCAO) and is related to the focal ischemia category. This technique produces consistent focal ischemia in a strictly defined region of the hemisphere and is less invasive than other methods. The procedure described is performed on mice, given the availability of several genetic variants and the high number of tests standardized for mice to aid in the behavioral and neurodeficit evaluation.
authors.affiliation: Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami; Miller School of Medicine, University of Miami.
authors.forename: Luc
authors.initials: L
authors.lastname: Bertrand
authors.affiliation: Miller School of Medicine, University of Miami.
authors.forename: Levi
authors.initials: L
authors.lastname: Dygert
authors.affiliation: Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami; Jerzy Kukuczka Academy of Physical Education; mtoborek@med.miami.edu.
authors.forename: Michal
authors.initials: M
authors.lastname: Toborek
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000175:diagnosis / Q000209:etiology*; D004195:Disease Models, Animal; D005455:Fluorescent Antibody Technique / Q000379:methods*; D020244:Infarction, Middle Cerebral Artery / Q000150:complications* / Q000175:diagnosis; D008297:Male; D051379:Mice; D015427:Reperfusion Injury / Q000175:diagnosis / Q000209:etiology*