title: 99mTc-Tetrofosmin Uptake Correlates with the Sensitivity of Glioblastoma Cell Lines to Temozolomide.
abstract: 99mTc-tetrofosmin (99mTc-TF) is a single-photon emission computed tomography tracer that has been used for brain tumor imaging. The aim of the study was to assess if99mTc-TF uptake by glioblastoma cells correlates with their response to temozolomide (TMZ). We investigated the correlation of TMZ antitumor effect with the99mTc-TF uptake in two glioblastoma cell lines. The U251MG cell line is sensitive to TMZ, whereas T98G is resistant. Viability and proliferation of the cells were examined by trypan blue exclusion assay and xCELLigence system. Cell cycle was analyzed with flow cytometry. The radioactivity in the cellular lysate was measured with a gamma scintillation counter. TMZ induced G2/M cell cycle arrest in U251MG cells, whereas there was no effect on cell cycle in T98G cells. Lower99mTc-TF uptake was observed in U251MG cells that were exposed to TMZ compared to control (P= 0.0159). No significant difference in respect to99mTc-TF uptake was found in T98G cells when exposed to TMZ compared to control (P= 0.8). With99mTc-TF, it was possible to distinguish between TMZ-sensitive and resistant glioblastoma cells within 6 h of treatment initiation. Thus,99mTc-TF uptake may consist a novel approach to assess an early response of glioblastoma to chemotherapy and deserves further investigation.
authors.affiliation: Neurosurgical Institute, University of Ioannina, Ioannina, Greece.
authors.forename: George A
authors.initials: GA
authors.lastname: Alexiou
authors.affiliation: Department of Nuclear Medicine, University Hospital of Ioannina, Ioannina, Greece.
authors.forename: Xanthi
authors.initials: X
authors.lastname: Xourgia
authors.affiliation: Neurosurgical Institute, University of Ioannina, Ioannina, Greece.
authors.forename: Paraskevi
authors.initials: P
authors.lastname: Gerogianni
authors.affiliation: Neurosurgical Institute, University of Ioannina, Ioannina, Greece.
authors.forename: Evrysthenis
authors.initials: E
authors.lastname: Vartholomatos
authors.affiliation: Department of Medical Physics, University of Ioannina, Ioannina, Greece.
authors.forename: John A
authors.initials: JA
authors.lastname: Kalef-Ezra
authors.affiliation: Department of Nuclear Medicine, University Hospital of Ioannina, Ioannina, Greece.
authors.forename: Andreas D
authors.initials: AD
authors.lastname: Fotopoulos
authors.affiliation: Neurosurgical Institute, University of Ioannina, Ioannina, Greece.
authors.forename: Athanasios P
authors.initials: AP
authors.lastname: Kyritsis
keywords: 99mTc-tetrofosmin; glioma; temozolomide