title: Mechanical Trap Surface-Enhanced Raman Spectroscopy for Three-Dimensional Surface Molecular Imaging of Single Live Cells.
abstract: Reported is a new shell-based spectroscopic platform, named mechanical trap surface-enhanced Raman spectroscopy (MTSERS), for simultaneous capture, profiling, and 3D microscopic mapping of the intrinsic molecular signatures on the membrane of single live cells. By leveraging the functionalization of the inner surfaces of the MTs with plasmonic gold nanostars, and conformal contact of the cell membrane, MTSERS permits excellent signal enhancement, reliably detects molecular signatures, and allows non-perturbative, multiplex 3D surface imaging of analytes, such as lipids and proteins on the surface of single cells. The demonstrated ability underscores the potential of MTSERS to perform 3D spectroscopic microimaging and to furnish biologically interpretable, quantitative, and dynamic molecular maps in live cell populations.
authors.affiliation: Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 3400 N. Charles St., Baltimore, MD, 21218, USA.
authors.forename: Qianru
authors.identifier: 0000-0003-3182-8188
authors.initials: Q
authors.lastname: Jin
authors.affiliation: School of Materials Science and Engineering, Central South University, Changsha, Hunan, 410083, China.|Department of Mechanical Engineering, The Johns Hopkins University, 3400 N. Charles St., Baltimore, MD, 21218, USA.
authors.forename: Ming
authors.identifier: 0000-0002-2289-0222
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 3400 N. Charles St., Baltimore, MD, 21218, USA.
authors.forename: Beril
authors.initials: B
authors.lastname: Polat
authors.affiliation: Department of Mechanical Engineering, The Johns Hopkins University, 3400 N. Charles St., Baltimore, MD, 21218, USA.
authors.forename: Santosh K
authors.identifier: 0000-0002-7034-586X
authors.initials: SK
authors.lastname: Paidi
authors.affiliation: Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 3400 N. Charles St., Baltimore, MD, 21218, USA.
authors.forename: Aimee
authors.identifier: 0000-0003-3548-3316
authors.initials: A
authors.lastname: Dai
authors.affiliation: Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 3400 N. Charles St., Baltimore, MD, 21218, USA.
authors.forename: Amy
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 3400 N. Charles St., Baltimore, MD, 21218, USA.
authors.forename: Jayson V
authors.identifier: 0000-0001-7332-0839
authors.initials: JV
authors.lastname: Pagaduan
authors.affiliation: Department of Mechanical Engineering, The Johns Hopkins University, 3400 N. Charles St., Baltimore, MD, 21218, USA.|Department of Oncology, The Johns Hopkins University, USA.
authors.forename: Ishan
authors.identifier: 0000-0003-0800-0825
authors.initials: I
authors.lastname: Barman
authors.affiliation: Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 3400 N. Charles St., Baltimore, MD, 21218, USA.|Department of Materials Science and Engineering, The Johns Hopkins University, USA.
authors.forename: David H
authors.identifier: 0000-0003-2735-4725
authors.initials: DH
authors.lastname: Gracias
mesh_terms: D001943:Breast Neoplasms / Q000000981:diagnostic imaging*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D057054:Molecular Imaging; D010316:Particle Size; D059010:Single-Cell Analysis; D013059:Spectrum Analysis, Raman; D013499:Surface Properties
keywords: Raman spectroscopy; biosensors; cancer; nanotechnology; surface plasmon resonance