title: Changes in hematological indices and lymphocyte subsets in response to whole blood donation in healthy male donors.
abstract: Whole blood donation has immunomodulatory effects, and most of these have been observed at short intervals following blood donation. This study aimed to investigate the impact of whole blood donation on lymphocyte subsets over a typical inter-donation interval. Healthy male subjects were recruited to study changes in complete blood count (CBC) (n = 42) and lymphocyte subsets (n = 16) before and at four intervals up to 106 days following blood donation. Repeated measures ANOVA were used to compare quantitative variables between different visits. Following blood donation, changes in CBC and erythropoietin were as expected. The neutrophil count increased by 11.3% at 8 days (p < .001). Novel changes were observed in lymphocyte subsets as the CD4/CD8 ratio increased by 9.2% (p < .05) at 8 days and 13.7% (p < .05) at 22 days. CD16-56 cells decreased by 16.2% (p < .05) at 8 days. All the subsets had returned to baseline by 106 days. Regression analysis showed that the changes in CD16-56 cells and CD4/CD8 ratio were not significant (Wilk's lambda = 0.15 and 0.94, respectively) when adjusted for BMI. In conclusion, following whole blood donation, there are transient changes in lymphocyte subsets. The effect of BMI on lymphocyte subsets and the effect of this immunomodulation on the immune response merit further investigation.
authors.affiliation: a King Abdullah International Medical Research Center , King Saud bin Abdulaziz University for Health Sciences, Pathology, King Abdulaziz Medical City , Jeddah , Saudi Arabia.
authors.forename: Anwar
authors.initials: A
authors.lastname: Borai
authors.affiliation: b Faculty of Health & Medical Sciences , University of Surrey , Guildford, Surrey , UK.
authors.forename: Callum
authors.initials: C
authors.lastname: Livingstone
authors.affiliation: a King Abdullah International Medical Research Center , King Saud bin Abdulaziz University for Health Sciences, Pathology, King Abdulaziz Medical City , Jeddah , Saudi Arabia.
authors.forename: Enaam
authors.initials: E
authors.lastname: Alsobhi
authors.affiliation: a King Abdullah International Medical Research Center , King Saud bin Abdulaziz University for Health Sciences, Pathology, King Abdulaziz Medical City , Jeddah , Saudi Arabia.
authors.forename: Abeer
authors.initials: A
authors.lastname: Al Sofyani
authors.affiliation: a King Abdullah International Medical Research Center , King Saud bin Abdulaziz University for Health Sciences, Pathology, King Abdulaziz Medical City , Jeddah , Saudi Arabia.
authors.forename: Dalal
authors.initials: D
authors.lastname: Balgoon
authors.affiliation: a King Abdullah International Medical Research Center , King Saud bin Abdulaziz University for Health Sciences, Pathology, King Abdulaziz Medical City , Jeddah , Saudi Arabia.
authors.forename: Anwar
authors.initials: A
authors.lastname: Farzal
authors.affiliation: a King Abdullah International Medical Research Center , King Saud bin Abdulaziz University for Health Sciences, Pathology, King Abdulaziz Medical City , Jeddah , Saudi Arabia.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Almohammadi
authors.affiliation: a King Abdullah International Medical Research Center , King Saud bin Abdulaziz University for Health Sciences, Pathology, King Abdulaziz Medical City , Jeddah , Saudi Arabia.
authors.forename: Abdulafattah
authors.initials: A
authors.lastname: Al-Amri
authors.affiliation: c Department of Clinical Biochemistry, Faculty of Medicine , King Abdulaziz University , Jeddah , Saudi Arabia.
authors.forename: Suhad
authors.initials: S
authors.lastname: Bahijri
authors.affiliation: a King Abdullah International Medical Research Center , King Saud bin Abdulaziz University for Health Sciences, Pathology, King Abdulaziz Medical City , Jeddah , Saudi Arabia.
authors.forename: Daad
authors.initials: D
authors.lastname: Alrowaili
authors.affiliation: a King Abdullah International Medical Research Center , King Saud bin Abdulaziz University for Health Sciences, Pathology, King Abdulaziz Medical City , Jeddah , Saudi Arabia.
authors.forename: Wafaa
authors.initials: W
authors.lastname: Bassiuni
authors.affiliation: a King Abdullah International Medical Research Center , King Saud bin Abdulaziz University for Health Sciences, Pathology, King Abdulaziz Medical City , Jeddah , Saudi Arabia.
authors.forename: Ayman
authors.initials: A
authors.lastname: Saleh
authors.affiliation: a King Abdullah International Medical Research Center , King Saud bin Abdulaziz University for Health Sciences, Pathology, King Abdulaziz Medical City , Jeddah , Saudi Arabia.
authors.forename: Norah
authors.initials: N
authors.lastname: Alrowaili
authors.affiliation: a King Abdullah International Medical Research Center , King Saud bin Abdulaziz University for Health Sciences, Pathology, King Abdulaziz Medical City , Jeddah , Saudi Arabia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Abdelaal
mesh_terms: D000328:Adult; D001782:Blood Donors; D015992:Body Mass Index; D016516:CD4-CD8 Ratio; D004921:Erythropoietin / Q000097:blood; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D016131:Lymphocyte Subsets; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D012044:Regression Analysis; D013997:Time Factors
chemical_list: D004921:Erythropoietin
keywords: B-lymphocyte subsets; Blood transfusion and donation; T-lymphocyte subsets; flow cytometry; immunomodulation