title: Chronic transgenerational vitamin B12 deficiency of severe and moderate magnitudes modulates adiposity-probable underlying mechanisms.
abstract: We have demonstrated previously that severe but not moderate vitamin B12 deficiency altered body composition and induced adiposity in female C57BL/6 mice. This study aims to elucidate the effects of chronic transgenerational dietary vitamin B12 restriction on body composition and various biochemical parameters in the F1 generation offspring of our mouse models of severe and moderate vitamin B12 deficiency established earlier. Female weanling C57BL/6 mice received, ad libitum, for 4 weeks a (i) control diet, (ii) vitamin B12-restricted diet with pectin as dietary fiber (severely deficient diet), or (iii) vitamin B12-restricted diet with cellulose as dietary fiber (moderately deficient diet) and then mated with control males. The offspring of control and severely deficient dams continued on the respective diets of their mothers. Few moderately deficient dams were rehabilitated to control diet from parturition and their pups were weaned to control diet. Also, some offspring born to moderately B12 deficient dams were weaned to control diet, while others continued on the same diet as their mothers. Various parameters were determined in the F1 offspring after 12 and 36 weeks of feeding. The results indicate that both severe and moderate maternal vitamin B12 restrictions were associated with accelerated catch-up growth, increased body fat percentage, visceral adiposity, dyslipidemia, fasting hyperglycemia and insulin resistance in the F1 offspring. Inflammation, increased glucocorticoid and oxidative stress and poor antioxidant defence probably underlie these adverse effects. Rehabilitation from parturition but not weaning was beneficial in delaying the onset of the adverse outcomes in the offspring. © 2016 BioFactors, 43(3):400-414, 2017.
authors.affiliation: Endocrinology and Metabolism Division, National Institute of Nutrition (NIN), ICMR, Hyderabad, 500007, India.
authors.forename: Shampa
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Endocrinology and Metabolism Division, National Institute of Nutrition (NIN), ICMR, Hyderabad, 500007, India.
authors.forename: Jitendra Kumar
authors.initials: JK
authors.lastname: Sinha
authors.affiliation: Endocrinology and Metabolism Division, National Institute of Nutrition (NIN), ICMR, Hyderabad, 500007, India.
authors.forename: Bojanapalli
authors.initials: B
authors.lastname: Muralikrishna
authors.affiliation: Pathology Division, National Institute of Nutrition (NIN), ICMR, Hyderabad, 500007, India.
authors.forename: Uday Kumar
authors.initials: UK
authors.lastname: Putcha
authors.affiliation: Endocrinology and Metabolism Division, National Institute of Nutrition (NIN), ICMR, Hyderabad, 500007, India.
authors.forename: Manchala
authors.initials: M
authors.lastname: Raghunath
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism / Q000473:pathology; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001823:Body Composition; D004032:Diet / Q000009:adverse effects / Q000379:methods; D004043:Dietary Fiber / Q000172:deficiency; D050171:Dyslipidemias / Q000209:etiology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006943:Hyperglycemia / Q000209:etiology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D040582:Inheritance Patterns; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000209:etiology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018384:Oxidative Stress; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D012720:Severity of Illness Index; D014805:Vitamin B 12 / Q000378:metabolism; D014806:Vitamin B 12 Deficiency / Q000150:complications / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D001786:Blood Glucose; D004043:Dietary Fiber; D007328:Insulin; D014805:Vitamin B 12
keywords: adipocytokine; dyslipidemia; insulin resistance; maternal vitamin B12 deficiency; oxidative stress