title: An Unusual Flavin-Dependent Halogenase from the Metagenome of the Marine Sponge Theonella swinhoei WA.
abstract: Uncultured bacteria from sponges have been demonstrated to be responsible for the generation of many potent, bioactive natural products including halogenated metabolites.1 The identification of gene clusters from the metagenomes of such bacterial communities enables the discovery of enzymes that mediate new and useful chemistries and allows insight to be gained into the biogenesis of potentially pharmacologically important natural products. Here we report a new pathway to the keramamides (krm); the first functional evidence for the existence of a distinct producer in the Theonella swinhoei WA chemotype is revealed, and a key enzyme on the pathway, a unique flavin-dependent halogenase with a broad substrate specificity, with potential as a useful new biocatalytic tool, is described.
authors.affiliation: School of Chemistry, University of St Andrews , St Andrews KY16 9ST, United Kingdom.
authors.forename: Duncan R M
authors.initials: DRM
authors.lastname: Smith
authors.affiliation: Institute of Microbiology, Eidgenössische Technische Hochschule Zürich , Vladimir-Prelog-Weg 4, 8093 Zurich, Switzerland.
authors.forename: Agustinus R
authors.identifier: 0000-0002-9223-7464
authors.initials: AR
authors.lastname: Uria
authors.affiliation: Institute of Microbiology, Eidgenössische Technische Hochschule Zürich , Vladimir-Prelog-Weg 4, 8093 Zurich, Switzerland.
authors.forename: Eric J N
authors.initials: EJN
authors.lastname: Helfrich
authors.affiliation: Technische Universität Dresden , 01062 Dresden, Germany.
authors.forename: Daniela
authors.initials: D
authors.lastname: Milbredt
authors.affiliation: Technische Universität Dresden , 01062 Dresden, Germany.
authors.forename: Karl-Heinz
authors.initials: KH
authors.lastname: van Pée
authors.affiliation: Institute of Microbiology, Eidgenössische Technische Hochschule Zürich , Vladimir-Prelog-Weg 4, 8093 Zurich, Switzerland.
authors.forename: Jörn
authors.initials: J
authors.lastname: Piel
authors.affiliation: School of Chemistry, University of St Andrews , St Andrews KY16 9ST, United Kingdom.
authors.forename: Rebecca J M
authors.identifier: 0000-0001-9869-8205
authors.initials: RJM
authors.lastname: Goss
mesh_terms: D000577:Amides / Q000378:metabolism; D000818:Animals; D001419:Bacteria / Q000235:genetics* / Q000378:metabolism; D053898:Biosynthetic Pathways; D005415:Flavins; D054879:Halogenation; D006867:Hydrolases / Q000378:metabolism; D054892:Metagenome / Q000235:genetics*; D005810:Multigene Family; D011161:Porifera / Q000382:microbiology; D047232:Theonella / Q000382:microbiology*
chemical_list: D000577:Amides; D005415:Flavins; D006867:Hydrolases