title: The relationship between Multidrug Resistance Protein 1(rs1045642) and Cholesterol 24-hydroxylase (rs754203) genes polymorphism with type 2 diabetes mellitus.
abstract: BACKGROUND
  The involvement of genetic factors like gene polymorphisms has been found to contribute significantly to the development and progression of type 2 diabetes (T2DM). Thousands of single nucleotide polymorphisms in various genes have been found to be associated with risk of T2DM. The present study was aimed to investigate association of Multidrug resistance 1 (MDR1) (rs1045642) and CYP46A1 (rs754203) genes polymorphism with T2DM.
  
  
  METHODS
  Study includes 333 subjects, 183 T2DM cases and 150 healthy controls. Single nucleotide polymorphism was evaluated by PCR-PFLP. Alleles and genotype frequencies between cases and controls were compared using χ2 and Student's t-tests. Odds ratios and 95% confidence intervals were calculated by logistic regression to assess the relative association between disease and genotypes.
  
  
  RESULTS
  In case of CYP46A1 gene, CC (p < 0.001) and CT (p = 0.001) genotypes and C allele (p < 0.001) were found to be a positive risk factor and TT genotype (p < 0.001) and T allele (p < 0.001) as negative risk factor for T2DM whereas, no association of MDR1 gene was found with T2DM (P values of all genotypes and alleles were greater than 0.001). MDR1 (rs1045642) and CYP46A1 (rs754203) genes polymorphism was not found associated with Fasting Blood Sugar (FBS), Diastolic Blood Pressure (DBP) and Systolic Blood Pressure (SBP).
  
  
  CONCLUSIONS
  CYP46A1 gene polymorphism is associated with the risk of T2DM whereas, MDR1 gene polymorphism was not found to confer any risk of T2DM in North Indian Ethnic group.
authors.affiliation: a Department of Biochemistry , Era's Lucknow Medical College and Hospital , Lucknow , India.
authors.forename: Saliha
authors.initials: S
authors.lastname: Rizvi
authors.affiliation: a Department of Biochemistry , Era's Lucknow Medical College and Hospital , Lucknow , India.
authors.forename: Syed Tasleem
authors.initials: ST
authors.lastname: Raza
authors.affiliation: b Department of Pathology , Era's Lucknow Medical College and Hospital , Lucknow , India.
authors.forename: S Riyaz
authors.initials: SR
authors.lastname: Mehdi
authors.affiliation: c Department of Medicine , Era's Lucknow Medical College and Hospital , Lucknow , India.
authors.forename: Zeba
authors.initials: Z
authors.lastname: Siddiqi
authors.affiliation: a Department of Biochemistry , Era's Lucknow Medical College and Hospital , Lucknow , India.
authors.forename: Ale
authors.initials: A
authors.lastname: Eba
authors.affiliation: a Department of Biochemistry , Era's Lucknow Medical College and Hospital , Lucknow , India.
authors.forename: Farzana
authors.initials: F
authors.lastname: Mahdi
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics*; D001786:Blood Glucose; D001794:Blood Pressure / Q000235:genetics; D000072556:Cholesterol 24-Hydroxylase / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D006801:Humans; D007194:India; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D001786:Blood Glucose; D000072556:Cholesterol 24-Hydroxylase
keywords: CYP46A1; MDR1; gene polymorphism; type 2 diabetes mellitus