title: A melamine based molecularly imprinted sensor for the determination of 8-hydroxydeoxyguanosine in human urine.
abstract: A sensitive and facile molecular imprinted sensor has been fabricated using edge plane pyrolytic graphite (EPPG) for the determination of an important oxidative DNA damage product, 8-hydroxydeoxyguanosine (8-OHdG). The molecularly imprinted polymer film was fabricated by electropolymerization of melamine in the presence of 8-OHdG, on glutaraldehyde/poly 1,5-diaminonaphthalene modified EPPG. The imprinted sensor surface was characterized by using Field Emission Scanning Electron Microscopy, Electron Impedance Spectroscopy, Cyclic Voltammetry, Square Wave Voltammetry and UV-visible spectroscopy. The calibration response was linear over a concentration range of 20×10-9-3×10-6M of 8-OHdG with sensitivity and limit of detection (3σ/b) as 10.59µM/µA and 3×10-9M respectively. The common metabolites in urine, like uric acid, ascorbic acid, xanthine, hypoxanthine do not interfere up to 100-fold concentration. The imprinted sensor is also successfully employed for the determination of 8-OHdG in human urine sample of a renal failure patient.
authors.affiliation: Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247 667, India.
authors.forename: Neeraj
authors.initials: N
authors.lastname: Kumar
authors.affiliation: Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247 667, India.
authors.lastname: Rosy
authors.affiliation: Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247 667, India. Electronic address: rngcyfcy@iitr.ac.in.
authors.forename: Rajendra N
authors.initials: RN
authors.lastname: Goyal
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000652:urine; D004563:Electrochemistry; D006801:Humans; D054802:Molecular Imprinting; D053761:Nanocomposites / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis; D014227:Triazines / Q000032:analysis*; D016482:Urinalysis / Q000379:methods*
chemical_list: D011108:Polymers; D014227:Triazines; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; C011907:melamine
keywords: 1,5-Diaminonaphthalene; 8-Hydroxydeoxyguanosine; Glutaraldehyde; Human urine; Melamine; Molecular imprinted technique