title: Advantage of Eu3+-Doped Polystyrene Microspheres Compared with Colloidal Gold Used in Immunochromatographic Assays for the Detection of Melamine in Milk.
abstract: Colloidal gold and Eu3+-doped fluorescent microspheres were applied as labels to develop the immunochromatographic strips for detecting melamine in milk. Under the optimized condition, the visual detection limit of colloidal gold-immunochromatographic test strip (ICTS) was 150 μg/L of melamine in phosphate-buffered saline (PBS), although the visual detection limit of fluorescent nanoparticles (FN)-ICTS was 75 μg/L in PBS. As thermal acceleration test, FN-ICTS could be stored at 37 °C for at least 11 d before sample testing, but the color of the lines on colloidal gold-ICTS faded away after 7-d storage. The visual result of FN-ICTS was more stable than that of colloidal gold-ICTS, and the fluorescence intensity of the line on FN-ICTS could be maintained up to 30 d at 22 °C after sample testing. Once the immunochromatographic strips were used to detect melamine in milk, no negative effect of milk components on the performance of FN-ICTS was identified, whereas the performance of colloidal gold-ICTS was significantly influenced by milk matrix.
authors.affiliation: College of Food Science & Project Engineering, Bohai Univ., Jinzhou, 121013, China.
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Tang
authors.affiliation: College of Food Science & Project Engineering, Bohai Univ., Jinzhou, 121013, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Food Science & Project Engineering, Bohai Univ., Jinzhou, 121013, China.
authors.forename: Xiuying
authors.initials: X
authors.lastname: Liu
authors.affiliation: Food, Nutrition, and Health Program, Faculty of Land and Food Systems, The Univ. of British Columbia, Vancouver, V6T 1Z4, Canada.
authors.forename: Evan
authors.initials: E
authors.lastname: Trofimchuk
authors.affiliation: Food, Nutrition, and Health Program, Faculty of Land and Food Systems, The Univ. of British Columbia, Vancouver, V6T 1Z4, Canada.
authors.forename: Shaolong
authors.initials: S
authors.lastname: Feng
authors.affiliation: College of Food Science & Project Engineering, Bohai Univ., Jinzhou, 121013, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Ma
authors.affiliation: College of Food Science & Project Engineering, Bohai Univ., Jinzhou, 121013, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Gao
authors.affiliation: College of Food Science & Project Engineering, Bohai Univ., Jinzhou, 121013, China.
authors.forename: Shubing
authors.initials: S
authors.lastname: Fang
authors.affiliation: Food, Nutrition, and Health Program, Faculty of Land and Food Systems, The Univ. of British Columbia, Vancouver, V6T 1Z4, Canada.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Lu
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D005063:Europium / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis*; D006046:Gold / Q000737:chemistry; D017979:Gold Colloid / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D008863:Microspheres; D008892:Milk / Q000737:chemistry*; D053758:Nanoparticles; D011137:Polystyrenes / Q000737:chemistry*; D011934:Reagent Strips / Q000737:chemistry*; D014227:Triazines / Q000737:chemistry*
chemical_list: D017979:Gold Colloid; D011137:Polystyrenes; D011934:Reagent Strips; D014227:Triazines; D005063:Europium; D006046:Gold; C011907:melamine
keywords: dairy products; food safety; immunochromatographic strip; rapid detection