title: Antidiabetic and anticancer activities ofMangifera indicacv. Okrong leaves.
abstract: Diabetes and cancer are a major global public health problem. Plant-derived agents with undesirable side-effects were required. This study aimed to evaluate antidiabetic and anticancer activities of the ethanolic leaf extract ofMangifera indicacv. Okrong and its active phytochemical compound, mangiferin. Antidiabetic activities against yeast α-glucosidase and rat intestinal α-glucosidase were determined using 1 mM of p-nitro phenyl-α-D-glucopyranoside as substrate. Inhibitory activity against porcine pancreatic α-amylase was performed using 1 mM of 2-chloro-4 nitrophenol-α-D-maltotroside-3 as substrate. Nitrophenol product was spectrophotometrically measured at 405 nm. Anticancer activity was evaluated against five human cancer cell lines compared to two human normal cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Mango leaf extract and mangiferin exhibited dose-dependent inhibition against yeast α-glucosidase with the IC50of 0.0503 and 0.5813 mg/ml, respectively, against rat α-glucosidase with the IC50of 1.4528 and 0.4333 mg/ml, respectively, compared to acarbose with the IC50of 11.9285 and 0.4493 mg/ml, respectively. For anticancer activity, mango leaf extract, at ≥200 μg/ml showed cytotoxic potential against all tested cancer cell lines. In conclusion, mango leaf possessed antidiabetic and anticancer potentialin vitro.
authors.affiliation: Public Health Sciences Programme, College of Public Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Aunyachulee
authors.initials: A
authors.lastname: Ganogpichayagrai
authors.affiliation: Public Health Sciences Programme, College of Public Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Chanida
authors.initials: C
authors.lastname: Palanuvej
authors.affiliation: Public Health Sciences Programme, College of Public Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand; Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmacy, Rangsit University, Pathum Thani 12000, Thailand.
authors.forename: Nijsiri
authors.initials: N
authors.lastname: Ruangrungsi
keywords: Anticancer; Mangifera indica L.; antidiabetic; mangiferin