title: Mössbauer Spectra of Mouse Hearts Reveal Age-dependent Changes in Mitochondrial and Ferritin Iron Levels.
abstract: Cardiac function requires continuous high levels of energy, and so iron, a critical player in mitochondrial respiration, is an important component of the heart. Hearts from57Fe-enriched mice were evaluated by Mössbauer spectroscopy. Spectra consisted of a sextet and two quadrupole doublets. One doublet was due to residual blood, whereas the other was due to [Fe4S4]2+clusters and low-spin FeIIhemes, most of which were associated with mitochondrial respiration. The sextet was due to ferritin; there was no evidence of hemosiderin, a ferritin decomposition product. Iron from ferritin was nearly absent in young hearts, but increased steadily with age. EPR spectra exhibited signals similar to those of brain, liver, and human cells. No age-dependent EPR trends were apparent. Hearts fromHFE-/-mice with hemochromatosis contained slightly more iron overall than controls, including more ferritin and less mitochondrial iron; these differences typify slightly older hearts, perhaps reflecting the burden due to this disease.HFE-/-livers were overloaded with ferritin but had low mitochondrial iron levels.IRP2-/-hearts contained less ferritin than controls but normal levels of mitochondrial iron. Hearts of young mice born to an iron-deficient mother contained normal levels of mitochondrial iron and no ferritin; the heart from the mother contained low ferritin and normal levels of mitochondrial iron. High-spin FeIIions were nearly undetectable in heart samples; these were evident in brains, livers, and human cells. Previous Mössbauer spectra of unenriched diseased human hearts lacked mitochondrial and blood doublets and included hemosiderin features. This suggests degradation of iron-containing species during sample preparation.
authors.affiliation: From the Departments of Chemistry and.
authors.forename: Joshua D
authors.initials: JD
authors.lastname: Wofford
authors.affiliation: From the Departments of Chemistry and.
authors.forename: Mrinmoy
authors.initials: M
authors.lastname: Chakrabarti
authors.affiliation: From the Departments of Chemistry and lindahl@chem.tamu.edu.|Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Lindahl
mesh_terms: D000367:Age Factors; D000818:Animals; D001923:Brain Chemistry; D004578:Electron Spin Resonance Spectroscopy; D005293:Ferritins / Q000032:analysis*; D006321:Heart; D006801:Humans; D007501:Iron / Q000032:analysis* / Q000378:metabolism; D008099:Liver / Q000737:chemistry; D051379:Mice; D008928:Mitochondria / Q000737:chemistry*; D009206:Myocardium / Q000737:chemistry* / Q000378:metabolism; D013048:Specimen Handling / Q000592:standards; D015204:Spectroscopy, Mossbauer / Q000379:methods*
chemical_list: D005293:Ferritins; D007501:Iron
keywords: Mossbauer spectroscopy; cardiac metabolism; electron paramagnetic resonance (EPR); iron; mitochondria