title: Isolation of Leptospira from blood culture bottles.
abstract: With the increasing use of real-time PCR techniques, Leptospira isolation has mostly been abandoned for the diagnosis of human leptospirosis. However, there is a great value of collecting Leptospira isolates to better understand the epidemiology of this complex zoonosis and to provide the researchers with different isolates. In this study, we have successfully isolated different Leptospira strains from BacT/Alert aerobic blood culture bottles and suggest that this privileged biological material offers an opportunity to isolate leptospires.
authors.affiliation: Institut Pasteur in New Caledonia, Institut Pasteur International Network, Leptospirosis Research and Expertise Unit, Noumea, New Caledonia.
authors.forename: Dominique
authors.initials: D
authors.lastname: Girault
authors.affiliation: Institut Pasteur in New Caledonia, Institut Pasteur International Network, Leptospirosis Research and Expertise Unit, Noumea, New Caledonia.
authors.forename: Marie-Estelle
authors.initials: ME
authors.lastname: Soupé-Gilbert
authors.affiliation: Institut Pasteur in New Caledonia, Institut Pasteur International Network, Leptospirosis Research and Expertise Unit, Noumea, New Caledonia.
authors.forename: Sophie
authors.initials: S
authors.lastname: Geroult
authors.affiliation: Institut Pasteur in New Caledonia, Institut Pasteur International Network, Medical Microbiology Unit, Noumea, New Caledonia.
authors.forename: Julien
authors.initials: J
authors.lastname: Colot
authors.affiliation: Institut Pasteur in New Caledonia, Institut Pasteur International Network, Leptospirosis Research and Expertise Unit, Noumea, New Caledonia. Electronic address: cgoarant@pasteur.nc.
authors.forename: Cyrille
authors.initials: C
authors.lastname: Goarant
mesh_terms: D000328:Adult; D001431:Bacteriological Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D000071997:Blood Culture; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007919:Leptospira / Q000302:isolation & purification*; D007922:Leptospirosis / Q000382:microbiology*
keywords: Blood bottles; Culture; Diagnosis; Leptospirosis