title: Survey for Zoonotic Microsporidian Pathogens in Wild Living Urban Rooks (Corvus frugilegus).
abstract: Microsporidia are opportunistic pathogens in nature infecting all animal phyla. There is a potential risk of microsporidian spores transmission from urban rooks inhabiting some metropolitan cities to people through casual interactions. The aim of this study was to identify microsporidia species in the droppings of rooks in Wroclaw, Poland. A total of 15 collective sets of droppings were examined using nested-PCR method. Amplification of ITS rRNA gene revealed the presence of Enterocytozoon bieneusi D, Peru 6, and Encephalitozoon hellem 1A genotypes. This study indicates that excreta of urban rooks can be an important source of human infection with these pathogens.
authors.affiliation: Department of Parasitology, Institute of Genetics and Microbiology, University of Wrocław, Przybyszewskiego 63/77, Wrocław, 51-148, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Perec-Matysiak
authors.affiliation: Department of Biology and Medical Parasitology, Wrocław Medical University, J. Mikulicza-Radeckiego 9, Wrocław, 50-367, Poland.
authors.forename: Maria
authors.identifier: 0000-0003-0643-6190
authors.initials: M
authors.lastname: Wesołowska
authors.affiliation: Department of Parasitology, Institute of Genetics and Microbiology, University of Wrocław, Przybyszewskiego 63/77, Wrocław, 51-148, Poland.
authors.forename: Kinga
authors.initials: K
authors.lastname: Leśniańska
authors.affiliation: Department of Parasitology, Institute of Genetics and Microbiology, University of Wrocław, Przybyszewskiego 63/77, Wrocław, 51-148, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Buńkowska-Gawlik
authors.affiliation: Department of Parasitology, Institute of Genetics and Microbiology, University of Wrocław, Przybyszewskiego 63/77, Wrocław, 51-148, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Hildebrand
authors.affiliation: Department of Biology and Medical Parasitology, Wrocław Medical University, J. Mikulicza-Radeckiego 9, Wrocław, 50-367, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Kicia
mesh_terms: D000818:Animals; D046368:Crows / Q000382:microbiology*; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D005243:Feces / Q000382:microbiology; D005838:Genotype; D016814:Microsporidia / Q000145:classification* / Q000235:genetics; D010802:Phylogeny; D011044:Poland; D016133:Polymerase Chain Reaction
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal
keywords: 
  Enterocytozoon bieneusi
  ; 
  ITS
  ; Encephalitozoon spp.; genotyping