title: Selection of transformation-efficient barley genotypes based on TFA (transformation amenability) haplotype and higher resolution mapping of the TFA loci.
abstract: The genetic substitution of transformation amenability alleles from 'Golden Promise' can facilitate the development of transformation-efficient lines from recalcitrant barley cultivars. Barley (Hordeum vulgare) cv. 'Golden Promise' is one of the most useful and well-studied cultivars for genetic manipulation. In a previous report, we identified several transformation amenability (TFA) loci responsible for Agrobacterium-mediated transformation using the F2generation of immature embryos, derived from 'Haruna Nijo' × 'Golden Promise,' as explants. In this report, we describe higher density mapping of these TFA regions with additional SNP markers using the same transgenic plants. To demonstrate the robustness of transformability alleles at the TFA loci, we genotyped 202 doubled haploid progeny from the cross 'Golden Promise' × 'Full Pint.' Based on SNP genotype, we selected lines having 'Golden Promise' alleles at TFA loci and used them for transformation. Of the successfully transformed lines, DH120366 came the closest to achieving a level of transformation efficiency comparable to 'Golden Promise.' The results validate that the genetic substitution of TFA alleles from 'Golden Promise' can facilitate the development of transformation-efficient lines from recalcitrant barley cultivars.
authors.affiliation: Institute of Plant Science and Resources, Okayama University, 2-20-1 Chuo, Kurashiki, Japan. hiroshi.hisano@rib.okayama-u.ac.jp.
authors.forename: Hiroshi
authors.identifier: http://orcid.org/0000-0001-8457-253X
authors.initials: H
authors.lastname: Hisano
authors.affiliation: Department Crop and Soil Sciences, Washington State University, Mount Vernon, WA, USA.
authors.forename: Brigid
authors.initials: B
authors.lastname: Meints
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich, UK.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Moscou
authors.affiliation: Department Genetica y Produccion Vegetal, Estacion Experimental de Aula Dei, Zaragoza, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Cistue
authors.affiliation: Department Genetica y Produccion Vegetal, Estacion Experimental de Aula Dei, Zaragoza, Spain.
authors.forename: Begoña
authors.initials: B
authors.lastname: Echávarri
authors.affiliation: Institute of Plant Science and Resources, Okayama University, 2-20-1 Chuo, Kurashiki, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Sato
authors.affiliation: Department Crop and Soil Science, Oregon State University, Corvallis, OR, USA.
authors.forename: Patrick M
authors.initials: PM
authors.lastname: Hayes
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D002874:Chromosome Mapping; D005838:Genotype; D006238:Haploidy; D006239:Haplotypes / Q000235:genetics*; D001467:Hordeum / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D012639:Seeds / Q000235:genetics; D014170:Transformation, Genetic / Q000235:genetics
chemical_list: D010940:Plant Proteins
keywords: Agrobacterium tumefaciens; Doubled haploid; Hordeum vulgare (barley); Single nucleotide polymorphism; Transformation