title: System-wide detection of protein-small molecule complexes suggests extensive metabolite regulation in plants.
abstract: Protein small molecule interactions are at the core of cell regulation controlling metabolism and development. We reasoned that due to the lack of system wide approaches only a minority of those regulatory molecules are known. In order to see whether or not this assumption is true we developed an effective approach for the identification of small molecules having potential regulatory role that obviates the need of protein or small molecule baits. At the core of this approach is a simple biochemical co-fractionation taking advantage of size differences between proteins and small molecules. Metabolomics based analysis of small molecules co-fractionating with proteins identified a multitude of small molecules in Arabidopsis suggesting the existence of numerous, small molecules/metabolites bound to proteins representing potential regulatory molecules. The approach presented here uses Arabidopsis cell cultures, but is generic and hence applicable to all biological systems.
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Veyel
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Kierszniowska
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany.
authors.forename: Monika
authors.initials: M
authors.lastname: Kosmacz
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany.
authors.forename: Ewelina Maria
authors.initials: EM
authors.lastname: Sokolowska
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany.
authors.forename: Aenne
authors.initials: A
authors.lastname: Michaelis
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany.
authors.forename: Marcin
authors.initials: M
authors.lastname: Luzarowski
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany.
authors.forename: Jagoda
authors.initials: J
authors.lastname: Szlachetko
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany.
authors.forename: Lothar
authors.initials: L
authors.lastname: Willmitzer
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Skirycz
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D002850:Chromatography, Gel; D008024:Ligands; D055442:Metabolome; D054852:Small Molecule Libraries; D049490:Systems Biology / Q000379:methods*
chemical_list: D008024:Ligands; D054852:Small Molecule Libraries