title: A novel and sensitive fluorescence sensor for glutathione detection by controlling the surface passivation degree of carbon quantum dots.
abstract: A novel fluorescence sensor based on controlling the surface passivation degree of carbon quantum dots (CQDs) was developed for glutathione (GSH) detection. First, we found that the fluorescence intensity of the CQDs which was obtained by directly pyrolyzing citric acid would increased largely after the surface passivation treatment by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC). In the light of this phenomenon, we designed a simple, rapid and selective fluorescence sensor based on the surface passivated CQDs. A certain and excess amount of EDC were mixed with GSH, part of EDC would form a stable complex with GSH owing to the exposed sulfhydryl group of GSH. As the synthesized CQDs were added into the above mixture solution, the fluorescence intensity of the (EDC/GSH)/CQDs mixture solution could be directly related to the amount of GSH. Compared to other fluorescence analytical methods, the fluorescence sensor we design is neither the traditional fluorescent "turn on" probes nor "turn off" probes. It is a new fluorescence analytical method that target object indirectly control the surface passivation degree of CQDs so that it can realize the detection of the target object. Moreover, the proposed method manifested great advantages including short analysis time, low cost and ease of operation.
authors.affiliation: Department of Chemistry and Laboratory for Preparation and Application of Ordered Structural Materials of Guangdong Province, Shantou University, Shantou, Guangdong 515063, PR China.
authors.forename: Jiahong
authors.initials: J
authors.lastname: Pan
authors.affiliation: National Detergent and Cosmetics Products Quality Supervision and Inspection Center (Guangdong), Shantou, Guangdong 515041, PR China.
authors.forename: Zengyao
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: National Detergent and Cosmetics Products Quality Supervision and Inspection Center (Guangdong), Shantou, Guangdong 515041, PR China.
authors.forename: Jianying
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Chemistry and Laboratory for Preparation and Application of Ordered Structural Materials of Guangdong Province, Shantou University, Shantou, Guangdong 515063, PR China.
authors.forename: Yaoyu
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Chemistry and Laboratory for Preparation and Application of Ordered Structural Materials of Guangdong Province, Shantou University, Shantou, Guangdong 515063, PR China.
authors.forename: Fushen
authors.initials: F
authors.lastname: Lu
authors.affiliation: Department of Chemistry and Laboratory for Preparation and Application of Ordered Structural Materials of Guangdong Province, Shantou University, Shantou, Guangdong 515063, PR China.
authors.forename: Yaowen
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Chemistry and Laboratory for Preparation and Application of Ordered Structural Materials of Guangdong Province, Shantou University, Shantou, Guangdong 515063, PR China. Electronic address: whgao@stu.edu.cn.
authors.forename: Wenhua
authors.initials: W
authors.lastname: Gao
mesh_terms: D002244:Carbon / Q000737:chemistry*; D005978:Glutathione / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D045663:Quantum Dots / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D013499:Surface Properties
chemical_list: D002244:Carbon; D005978:Glutathione
keywords: Fluorescence; GSH; Surfaced passivation carbon quantum dots