title: Starch-enhanced degradation of HMW PAHs by Fusarium sp. in an aged polluted soil from a coal mining area.
abstract: The present study used strain ZH-H2 (Fusarium sp.) isolated by our group as the PAH-degrading strain and 5-6-rings PAHs as degradation objects. The soil incubation experiment was carried out to investigate the starch-enhanced degradation effects of HMW PAHs by Fusarium sp. in an Aged Polluted Soil from a Coal Mining Area. The results showed that the removal rates of BaP, InP and BghiP increased with increasing inoculation rate of ZH-H2 in the unsterile aged polluted soil of coal mining area, with the exception of BbF degradation which increased in the H2 treatment and then decreased. Different addition dosage of starch apparently resulted in degradation of 4 PAHs in soil, with removal rates of 14.47% for BaP, 23.83% for DbA, 30.77% for BghiP and 31.00% for InP obtained with treatment D2, respectively higher than in treatment D1. So starch addition apparently enhanced the degradation of the 4 PAHs, especially InP and BghiP, by native microbes in the aged HMW PAH-polluted soil. By adding starch to these aged polluted soils with inoculated strain ZH-H2, HMW-PAHs degradation was further improved and addition of 0.5 g kg-1starch to soils with 1.0 g kg-1Fusarium ZH-H2 (D2+ H2) performed best to the 4 HMW-PAHs in all of these combination treatments by a factor of up to 3.09, depending on the PAH. We found that the highest polyphenol oxidase activities under D2+ H2treatments are consistent with the results of removal rates of 4 PAHs. Our findings suggest that the combination of Fusarium sp. ZH-H2 and starch offers a suitable alternative for bioremediation of aged PAH-contaminated soil in coal mining areas, with a recommended inoculation size of 0.5 g Fusarium sp. ZH-H2 and addition of 0.5 g kg-1starch per kg soil.
authors.affiliation: Key Laboratory for Farmland Eco-Environment, Hebei Province and College of Resource and Environmental Sciences, Agricultural University of Hebei, Baoding, PR China; Hebei Academy of Agriculture and forestry Sciences, Shijiazhuang, 050050, P.R.China.
authors.forename: Ou-Ya
authors.initials: OY
authors.lastname: Zhao
authors.affiliation: Key Laboratory for Farmland Eco-Environment, Hebei Province and College of Resource and Environmental Sciences, Agricultural University of Hebei, Baoding, PR China.
authors.forename: Xue-Na
authors.initials: XN
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Farmland Eco-Environment, Hebei Province and College of Resource and Environmental Sciences, Agricultural University of Hebei, Baoding, PR China.
authors.forename: Sheng-Dong
authors.initials: SD
authors.lastname: Feng
authors.affiliation: Key Laboratory for Farmland Eco-Environment, Hebei Province and College of Resource and Environmental Sciences, Agricultural University of Hebei, Baoding, PR China.
authors.forename: Li-Xiu
authors.initials: LX
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Farmland Eco-Environment, Hebei Province and College of Resource and Environmental Sciences, Agricultural University of Hebei, Baoding, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.affiliation: Key Laboratory for Farmland Eco-Environment, Hebei Province and College of Resource and Environmental Sciences, Agricultural University of Hebei, Baoding, PR China. Electronic address: yangzhixin@126.com.
authors.forename: Zhi-Xin
authors.initials: ZX
authors.lastname: Yang
authors.affiliation: Key Laboratory for Farmland Eco-Environment, Hebei Province and College of Resource and Environmental Sciences, Agricultural University of Hebei, Baoding, PR China.
authors.forename: Miao-Miao
authors.initials: MM
authors.lastname: Chen
authors.affiliation: Key Laboratory for Farmland Eco-Environment, Hebei Province and College of Resource and Environmental Sciences, Agricultural University of Hebei, Baoding, PR China.
authors.forename: Xue-Dan
authors.initials: XD
authors.lastname: Fang
mesh_terms: D001673:Biodegradation, Environmental / Q000187:drug effects; D003032:Coal Mining; D005670:Fusarium / Q000187:drug effects* / Q000378:metabolism*; D008970:Molecular Weight; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry* / Q000378:metabolism*; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000737:chemistry / Q000378:metabolism; D013213:Starch / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D012987:Soil; D012989:Soil Pollutants; D013213:Starch
keywords: Bioremediation; Fusarium sp.; HMW PAHs; Polyphenol oxidase activities; Starch