title: Engineering of Radioiodine-Labeled Gold Core-Shell Nanoparticles As Efficient Nuclear Medicine Imaging Agents for Trafficking of Dendritic Cells.
abstract: The development of highly sensitive, stable, and biocompatible imaging agents allowing visualization of dendritic cell (DC) migration is one of the essential factors for effective DC-based immunotherapy. Here, we used a novel and efficient synthesis approach to develop radioiodine-124-labeled tannic acid gold core-shell nanoparticles (124I-TA-Au@AuNPs) for DC labeling and in vivo tracking of their migration using positron emission tomography (PET).124I-TA-Au@AuNPs were produced within 40 min in high yield via straightforward tannic acid-mediated radiolabeling chemistry and incorporation of Au shell, which resulted in high radio-sensitivity and excellent chemical stability of nanoparticles in DCs and living mice.124I-TA-Au@AuNPs demonstrated good DC labeling efficiency and did not affect cell biological functions, including proliferation and phenotype marker expression. Importantly,124I-TA-Au@AuNPs in an extremely low amount (0.1 mg/kg) were successfully applied to track the migration of DCs to lymphoid organs (draining lymph nodes) in mice.
authors.forename: Sang Bong
authors.initials: SB
authors.lastname: Lee
authors.forename: Sang-Woo
authors.initials: SW
authors.lastname: Lee
authors.forename: Shin Young
authors.initials: SY
authors.lastname: Jeong
authors.forename: GhilSuk
authors.initials: G
authors.lastname: Yoon
authors.affiliation: Daegu-Gyeongbuk Medical Innovation Foundation , Daegu 41061, Korea.
authors.forename: Sung Jin
authors.identifier: http://orcid.org/0000-0002-4786-8830
authors.initials: SJ
authors.lastname: Cho
authors.forename: Sang Kyoon
authors.initials: SK
authors.lastname: Kim
authors.forename: In-Kyu
authors.initials: IK
authors.lastname: Lee
authors.forename: Byeong-Cheol
authors.initials: BC
authors.lastname: Ahn
authors.affiliation: Daegu-Gyeongbuk Medical Innovation Foundation , Daegu 41061, Korea.
authors.forename: Jaetae
authors.initials: J
authors.lastname: Lee
authors.forename: Yong Hyun
authors.identifier: http://orcid.org/0000-0003-3161-7698
authors.initials: YH
authors.lastname: Jeon
mesh_terms: D000818:Animals; D003713:Dendritic Cells; D006046:Gold; D007457:Iodine Radioisotopes; D053768:Metal Nanoparticles; D051379:Mice; D009683:Nuclear Medicine
chemical_list: D007457:Iodine Radioisotopes; C000614959:Iodine-124; D006046:Gold
keywords: PET imaging; cell tracking; dendritic cells; detection limit; functionalized gold core−shell nanoparticles; tannic acid