title: Geosmin-producing Species of Coelosphaerium (Synechococcales, Cyanobacteria) in Lake Shinji, Japan.
abstract: In Lake Shinji, Japan, periodic outbreaks of musty odour have occurred since mid-May 2007. Although the substance responsible for the odour was identified as geosmin, the odour-producing organism was unknown. We cultivated an axenic unialgal strain and determined that a species of Coelosphaerium (Synechococcales) was responsible for the production of geosmin in Lake Shinji. Our analysis was conducted using gas chromatography/mass spectrometry to determine the odorous compound. To determine the algae species, it was observed by optical microscopy to describe its morphological characteristics and the polymerase chain reaction was used to characterise the nucleotide sequence of the 16S rRNA gene and the 16S-23S rRNA internal transcribed spacer region. In addition, we explored the relationship between the number of cells of the Coelosphaerium sp. and the concentration of geosmin. In conclusion, geosmin, the cause of the musty odour in Lake Shinji in autumn 2009, was produced by Coelosphaerium sp., and to our knowledge, this is the first report of a geosmin-producing species in the family Coelosphaeriaceae.
authors.affiliation: Shimane Prefectural Institute of Public Health and Environmental Science, 582-1 Nishihamasada, Matsue, Shimane 690-0122, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Godo
authors.affiliation: Shimane Prefectural Institute of Public Health and Environmental Science, 582-1 Nishihamasada, Matsue, Shimane 690-0122, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Saki
authors.affiliation: Shimane Prefectural Institute of Public Health and Environmental Science, 582-1 Nishihamasada, Matsue, Shimane 690-0122, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nojiri
authors.affiliation: The United Graduate School of Agricultural Sciences, Tottori University, 4-101 Koyama-cho minami, Tottori, Tottori 680-8553, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tsujitani
authors.affiliation: Graduate School of Science and Engineering, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 690-8504, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Sugahara
authors.affiliation: Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 690-8504, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Hayashi
authors.affiliation: Shimane Prefectural Institute of Public Health and Environmental Science, 582-1 Nishihamasada, Matsue, Shimane 690-0122, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kamiya
authors.affiliation: Faculty of Education, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 690-8504, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ohtani
authors.affiliation: Graduate School of Science and Engineering, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 690-8504, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Seike
mesh_terms: D000458:Cyanobacteria / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D060106:Lakes / Q000382:microbiology; D009284:Naphthols / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics
chemical_list: D009284:Naphthols; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; C001278:geosmin