title: mRNA-miRNA integrative analysis of diabetes-induced cardiomyopathy in rats.
abstract: An integrative analysis of miRNA and mRNA expression profiles in left ventricle (LV) of diabetes-induced rats was performed to elucidate the role of miRNAs and their mRNAs target in diabetic cardiomyopathy (DCM). mRNA (GSE4745) and miRNA (GSE44179) datasets were downloaded from Gene Expression Omnibus 2R (GEO2R) and differentially expressed mRNAs and miRNAs were selected. Cardiotoxicity-related mRNAs (n=7) were analyzed by Ingenuity Pathway Analyses 6 (IPA) and regulatory miRNAs (n=639) were identified using TargetScan 7.1. web dataset. The integrative analysis was performed between miRNAs differentially expressed in GSE44179 and regulatory TargetScan-detected miRNAs of mRNAs differentially expressed in GSE4745.Pla2g2aandHk2mRNAs were up-and-down regulated, respectively, in GSE4745 on days 3 and 42 after diabetes-induction. ThePla2g2aregulatory miRNAs, rno-miR-877, rno-miR-320 and rno-miR-214,were down-regulated, andHk2regulatory miRNAs, rno-miR-17, rno-miR-187, rno-miR-34a, rno-miR-322, rno-miR-188, rno-miR-532 and rno-miR-21, were up-regulated in GSE44179 dataset. These results are suggestive thatPla2g2aandHk2mRNAs and their regulatory miRNAs play a role in DCM pathogenesis and they may be potential circulating biomarkers to detect early cardiovascular complications in diabetic patients.
authors.affiliation: Department of Clinical and Toxicological Analyses, Federal University of Rio Grande do Norte. General Cordeiro de Farias Av., Natal, Rio Grande do Norte, 59012-570, Brazil.
authors.forename: Mariana B
authors.initials: MB
authors.lastname: Lopes
authors.affiliation: Department of Clinical and Toxicological Analyses, Federal University of Rio Grande do Norte. General Cordeiro de Farias Av., Natal, Rio Grande do Norte, 59012-570, Brazil.
authors.forename: Renata C
authors.initials: RC
authors.lastname: Freitas
authors.affiliation: Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of Sao Paulo. 580 B17 Lineu Prestes Av., Butantan,05508-900. Sao Paulo, Brazil.
authors.forename: Mario H
authors.initials: MH
authors.lastname: Hirata
authors.affiliation: Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of Sao Paulo. 580 B17 Lineu Prestes Av., Butantan,05508-900. Sao Paulo, Brazil.
authors.forename: Rosario
authors.initials: R
authors.lastname: Hirata
authors.affiliation: Department of Clinical and Toxicological Analyses, Federal University of Rio Grande do Norte. General Cordeiro de Farias Av., Natal, Rio Grande do Norte, 59012-570, Brazil.
authors.forename: Adriana A
authors.initials: AA
authors.lastname: Rezende
authors.affiliation: Department of Clinical and Toxicological Analyses, Federal University of Rio Grande do Norte. General Cordeiro de Farias Av., Natal, Rio Grande do Norte, 59012-570, Brazil.
authors.forename: Vivian N
authors.initials: VN
authors.lastname: Silbiger
authors.affiliation: Department of Clinical and Toxicological Analyses, Federal University of Rio Grande do Norte. General Cordeiro de Farias Av., Natal, Rio Grande do Norte, 59012-570, Brazil.
authors.forename: Raul H
authors.initials: RH
authors.lastname: Bortolin
authors.affiliation: Department of Clinical and Toxicological Analysis, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil. Av. General Cordeiro de Farias, S/N - CEP: 59012-570, andre.luchessi@outlook.com.
authors.forename: Andre D
authors.initials: AD
authors.lastname: Luchessi
mesh_terms: D000818:Animals; D009202:Cardiomyopathies / Q000235:genetics* / Q000378:metabolism; D003920:Diabetes Mellitus / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger