title: Combined Raman and autofluorescence ex vivo diagnostics of skin cancer in near-infrared and visible regions.
abstract: The differentiation of skin melanomas and basal cell carcinomas (BCCs) was demonstrated based on combined analysis of Raman and autofluorescence spectra stimulated by visible and NIR lasers. It was ex vivo tested on 39 melanomas and 40 BCCs. Six spectroscopic criteria utilizing information about alteration of melanin, porphyrins, flavins, lipids, and collagen content in tumor with a comparison to healthy skin were proposed. The measured correlation between the proposed criteria makes it possible to define weakly correlated criteria groups for discriminant analysis and principal components analysis application. It was shown that the accuracy of cancerous tissues classification reaches 97.3% for a combined 6-criteria multimodal algorithm, while the accuracy determined separately for each modality does not exceed 79%. The combined 6-D method is a rapid and reliable tool for malignant skin detection and classification.
authors.affiliation: Samara National Research University, Department of Laser and Biotechnical Systems, Samara, Russia.
authors.forename: Ivan A
authors.initials: IA
authors.lastname: Bratchenko
authors.affiliation: Samara National Research University, Department of Laser and Biotechnical Systems, Samara, Russia.
authors.forename: Dmitry N
authors.initials: DN
authors.lastname: Artemyev
authors.affiliation: Samara National Research University, Department of Laser and Biotechnical Systems, Samara, Russia.
authors.forename: Oleg O
authors.initials: OO
authors.lastname: Myakinin
authors.affiliation: Samara National Research University, Department of Laser and Biotechnical Systems, Samara, Russia.
authors.forename: Yulia A
authors.initials: YA
authors.lastname: Khristoforova
authors.affiliation: Samara State Medical University, Department of Oncology, Samara, Russia.
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Moryatov
authors.affiliation: Samara State Medical University, Department of Oncology, Samara, Russia.
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Kozlov
authors.affiliation: Samara National Research University, Department of Laser and Biotechnical Systems, Samara, Russia.
authors.forename: Valery P
authors.initials: VP
authors.lastname: Zakharov
mesh_terms: D002280:Basal Cell Carcinoma / Q000000981:diagnostic imaging; D016002:Discriminant Analysis; D006801:Humans; D007259:Infrared Rays; D008027:Light; D008545:Melanoma / Q000000981:diagnostic imaging; D012878:Skin Neoplasms / Q000000981:diagnostic imaging*; D013059:Spectrum Analysis, Raman