title: Nardostachys jatamansiRoot Extract Modulates the Growth of IMR-32 and SK-N-MC Neuroblastoma Cell Lines ThroughMYCNMediated Regulation of MDM2 and p53.
abstract: OBJECTIVE
  The present study evaluated the effect of ethanolic extract ofNardostachys jatamansiroots (NJet) on MYCN mediated regulation of expression of MDM2 and p53 proteins in neuroblastoma cell lines, IMR-32 and SK-N-MC.
  
  
  METHODS
  The effect of NJeton cell viability was determined by MTT; and on growth kinetics was evaluated by trypan blue dye exclusion method and soft agar assay. The expression of p53, MDM2 and MYCN proteins in response to NJettreatment was evaluated by immunoblotting.
  
  
  RESULTS
  NJetdecreased the viability of neuroblastoma cells without affecting the viability of non-cancerous, HEK-293 cells. It altered the growth kinetics of the cancer cells in a dose-dependent manner. NJetdown regulated the expression of MYCN and MDM2 proteins with a simultaneous increase in the expression of tumor suppressor protein p53.
  
  
  CONCLUSIONS
  The present data demonstrated that NJetregulated the growth of IMR-32 and SK-N-MC through reduction in MYCN expression that lead to down regulation of MDM2 protein and increase in p53 expression. These preliminary results warrant further in depth studies to explore the therapeutic potential ofNardostachys jatamansiin the management of neuroblastoma.
  
  
  CONCLUSIONS
  NJetreduced the viability of human neuroblastoma cell lines without affecting the viability of non-cancerous, HEK-293 cells.NJetregulated the growth kinetics of the cancer cells.NJetdecreased the expression of MYCN and MDM2 proteins and simultaneously increased the expression of tumor suppressor protein p53.Abbreviation used:NJet: Ethanolic extract ofNardostachys jatamansiMTT: 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide HPTLC: High performance thin layer chromatography.
authors.affiliation: Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth University, Katraj-Dhankawadi, Pune, Maharashtra, India.
authors.forename: Snehal
authors.initials: S
authors.lastname: Suryavanshi
authors.affiliation: Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth University, Katraj-Dhankawadi, Pune, Maharashtra, India.
authors.forename: Prerna
authors.initials: P
authors.lastname: Raina
authors.affiliation: Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth University, Katraj-Dhankawadi, Pune, Maharashtra, India.
authors.forename: Rashmi
authors.initials: R
authors.lastname: Deshpande
authors.affiliation: Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth University, Katraj-Dhankawadi, Pune, Maharashtra, India.
authors.forename: Ruchika
authors.initials: R
authors.lastname: Kaul-Ghanekar
keywords: MDM2; MYCN; Nardostachys jatamansi; Neuroblastoma; p53