title: Detection of Brucella sp. infection through serological, microbiological, and molecular methods applied to buffaloes in Maranhão State, Brazil.
abstract: The aim of the current study is to diagnose Brucella spp. infection using methods such as serology, bacterial isolation, and molecular analysis in buffaloes bred in Maranhão State. In order to do so, 390 samples of buffalo serum were subjected to serological tests, to Rose Bengal Plate Test (RBPT) and to 2-mercaptoethanol (2-ME) combined with slow agglutination test (SAT). Vaginal swabs were collected from seropositive animals and subjected to bacterial isolation and to generic PCR. According to the serological test, 16 animals had a positive reaction to the confirmatory test (2-ME/SAT). As for bacterial isolation, three samples resulted in the isolation of Brucella spp.-characteristic colonies, which were confirmed through PCR. These results confirmed Brucella spp. infection in the buffalo herd from Maranhão State.
authors.affiliation: Doutorado em Rede de Biodiversidade e Biotecnologia da Amazônia Legal, Universidade Estadual do Maranhão, São Luís, MA, Brazil.
authors.forename: Larissa Sarmento
authors.initials: LS
authors.lastname: Dos Santos
authors.affiliation: Departamento do Curso de Medicina, Universidade Ceuma, São Luís, MA, Brazil.
authors.forename: Joicy Cortez
authors.initials: JC
authors.lastname: Sá
authors.affiliation: Doutorado em Rede de Biodiversidade e Biotecnologia da Amazônia Legal, Universidade Estadual do Maranhão, São Luís, MA, Brazil.
authors.forename: Diego Luiz
authors.initials: DL
authors.lastname: Dos Santos Ribeiro
authors.affiliation: Universidade Estadual do Maranhão, São Luís, MA, Brazil.
authors.forename: Nancyleni Pinto
authors.initials: NP
authors.lastname: Chaves
authors.affiliation: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Juliana Pinto
authors.initials: JP
authors.lastname: da Silva Mol
authors.affiliation: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Renato Lima
authors.initials: RL
authors.lastname: Santos
authors.affiliation: Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Tatiane Alves
authors.initials: TA
authors.lastname: da Paixão
authors.affiliation: Doutorado em Rede de Biodiversidade e Biotecnologia da Amazônia Legal, Universidade Estadual do Maranhão, São Luís, MA, Brazil. alcinavcn@yahoo.com.|Universidade Estadual do Maranhão, São Luís, MA, Brazil. alcinavcn@yahoo.com.
authors.forename: Alcina Vieira
authors.initials: AV
authors.lastname: de Carvalho Neta
mesh_terms: D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001938:Brazil; D002002:Brucella / Q000302:isolation & purification*; D002006:Brucellosis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D002020:Buffaloes / Q000382:microbiology*; D005260:Female; D008623:Mercaptoethanol; D016133:Polymerase Chain Reaction; D012395:Rose Bengal; D012698:Serologic Tests / Q000379:methods
chemical_list: D000907:Antibodies, Bacterial; D012395:Rose Bengal; D008623:Mercaptoethanol
keywords: Brucella spp.; Buffalo; Isolation; PCR