title: Association of seven renin angiotensin system gene polymorphisms with restenosis in patients following coronary stenting.
abstract: OBJECTIVE
  Percutaneous coronary intervention, despite being effective for coronary revascularization, causes in-stent restenosis due to neointimal hyperplasia in a large number of patients. The renin-angiotensin system is involved in neointimal hyperplasia. This study sought to evaluate seven gene polymorphisms of key renin-angiotensin system components, including angiotensinogen, angiotensin-converting enzyme and angiotensin II type 1a receptors, and their associations with in-stent restenosis in patients with coronary artery disease following coronary stenting.
  
  
  RESULTS
  Three hundred and fifty-two patients undergoing coronary drug-eluting stent implantation were recruited. Seventy-five patients (21.3%) were diagnosed as restenosis by angiography. Genotyping for angiotensin-converting enzyme insertion/deletion demonstrated a significant association of angiotensin-converting enzyme DD genotype with the occurrence of restenosis. Direct DNA sequencing revealed no association of angiotensinogen (M235T, G217A, G152A, G-6A, and A-20C) or angiotensin II type I receptor A1166C polymorphisms with in-stent restenosis. However, angiotensin II type 1a A1166C polymorphism was significantly associated with increased susceptibility to restenosis in a subgroup of patients aged more than 60 years.
  
  
  CONCLUSIONS
  Thus, our study suggests that genetic polymorphisms of angiotensin-converting enzyme insertion/deletion are associated with in-stent restenosis in coronary artery disease patients following coronary stenting.
authors.affiliation: 1 Laboratory of Cardiovascular Disease, Taizhou Hospital, Wenzhou Medical University, China.|2 Enze Medical Research Center, Taizhou Hospital, Wenzhou Medical University, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
authors.affiliation: 1 Laboratory of Cardiovascular Disease, Taizhou Hospital, Wenzhou Medical University, China.|3 Department of Cardiology, Taizhou Hospital, Wenzhou Medical University, China.
authors.forename: Minjun
authors.initials: M
authors.lastname: Yang
authors.affiliation: 1 Laboratory of Cardiovascular Disease, Taizhou Hospital, Wenzhou Medical University, China.|3 Department of Cardiology, Taizhou Hospital, Wenzhou Medical University, China.
authors.forename: Jiangbo
authors.initials: J
authors.lastname: Lin
authors.affiliation: 1 Laboratory of Cardiovascular Disease, Taizhou Hospital, Wenzhou Medical University, China.|3 Department of Cardiology, Taizhou Hospital, Wenzhou Medical University, China.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Zhu
authors.affiliation: 1 Laboratory of Cardiovascular Disease, Taizhou Hospital, Wenzhou Medical University, China.|3 Department of Cardiology, Taizhou Hospital, Wenzhou Medical University, China.
authors.forename: Yifei
authors.initials: Y
authors.lastname: Lu
authors.affiliation: 1 Laboratory of Cardiovascular Disease, Taizhou Hospital, Wenzhou Medical University, China.|3 Department of Cardiology, Taizhou Hospital, Wenzhou Medical University, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: 1 Laboratory of Cardiovascular Disease, Taizhou Hospital, Wenzhou Medical University, China.|3 Department of Cardiology, Taizhou Hospital, Wenzhou Medical University, China.
authors.forename: Yinshen
authors.initials: Y
authors.lastname: Xue
authors.affiliation: 1 Laboratory of Cardiovascular Disease, Taizhou Hospital, Wenzhou Medical University, China.|3 Department of Cardiology, Taizhou Hospital, Wenzhou Medical University, China.
authors.forename: Congfeng
authors.initials: C
authors.lastname: Fang
authors.affiliation: 1 Laboratory of Cardiovascular Disease, Taizhou Hospital, Wenzhou Medical University, China.|3 Department of Cardiology, Taizhou Hospital, Wenzhou Medical University, China.
authors.forename: Lijiang
authors.initials: L
authors.lastname: Tang
authors.affiliation: 4 Department of Surgery, Stanford University School of Medicine, USA.
authors.forename: Baohui
authors.initials: B
authors.lastname: Xu
authors.affiliation: 1 Laboratory of Cardiovascular Disease, Taizhou Hospital, Wenzhou Medical University, China.|3 Department of Cardiology, Taizhou Hospital, Wenzhou Medical University, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Jiang
authors.affiliation: 1 Laboratory of Cardiovascular Disease, Taizhou Hospital, Wenzhou Medical University, China.|2 Enze Medical Research Center, Taizhou Hospital, Wenzhou Medical University, China.|3 Department of Cardiology, Taizhou Hospital, Wenzhou Medical University, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Chen
mesh_terms: D000368:Aged; D000808:Angiotensinogen / Q000235:genetics; D001483:Base Sequence; D023903:Coronary Restenosis / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D056726:Genetic Association Studies; D006801:Humans; D015810:Linkage Disequilibrium / Q000235:genetics; D008297:Male; D008875:Middle Aged; D007703:Peptidyl-Dipeptidase A / Q000235:genetics; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics; D012084:Renin-Angiotensin System / Q000235:genetics*; D015607:Stents
chemical_list: D044140:Receptor, Angiotensin, Type 1; D000808:Angiotensinogen; D007703:Peptidyl-Dipeptidase A
keywords: Coronary artery disease; renin angiotensin system; restenosis; single nucleotide polymorphism