title: Biochemical composition of symplastic sap from sugarcane genetically modified to overproduce proline.
abstract: Global interest in sugarcane has increased significantly in recent years because of its economic impact on sustainable energy production. The purpose of the present study was to evaluate changes in the concentrations of total sugars, amino acids, free proline, and total proteins by colorimetric analyses and nuclear magnetic resonance (NMR) to perform a metabolic profiling of a water-soluble fraction of symplastic sap in response to the constitutive expression of a mutant Δ1-pyrroline-5-carboxylate synthetase (P5CS) gene from Vigna aconitifolia. However, there was not a significant increase in the free proline content in the sap of transgenic plants compared to the non-transformed control plants. The most noticeable difference between the two genotypes was an almost two-fold increase in the accumulation of sucrose in the stem internodes of P5CS transgenic sugarcane plants. The results presented in this work showed that transgenic sugarcane plants with increased levels of free proline accumulates high soluble sugar content and, therefore, may represent a novel genotype for improving sugarcane cultivars.
authors.affiliation: Department of Pharmacy, Graduate Program in Pharmaceutical Sciences, State University of Maringá, Avenida Colombo 5790, 87.020-900 Maringá, PR, Brazil.
authors.forename: Graciele Carraro
authors.initials: GC
authors.lastname: Balestro
authors.affiliation: Department of Pharmacy, Graduate Program in Pharmaceutical Sciences, State University of Maringá, Avenida Colombo 5790, 87.020-900 Maringá, PR, Brazil.
authors.forename: Bruna
authors.initials: B
authors.lastname: Higashi
authors.affiliation: Department of Pharmacy, Graduate Program in Pharmaceutical Sciences, State University of Maringá, Avenida Colombo 5790, 87.020-900 Maringá, PR, Brazil.
authors.forename: Sheila Mara Sanches
authors.initials: SM
authors.lastname: Lopes
authors.affiliation: Program of Master in Health Promotion and Program of Master in Clean Technologies, University Center of Maringá, Avenida Guedner, 1610, 87050-900 Maringá, PR, Brazil.
authors.forename: José Eduardo
authors.initials: JE
authors.lastname: Gonçalves
authors.affiliation: Universidade do Oeste Paulista (UNOESTE), Rodovia Raposo Tavares, km 572, 19.067-175 Presidente Prudente, SP, Brazil.
authors.forename: Luiz Gonzaga Esteves
authors.initials: LG
authors.lastname: Vieira
authors.affiliation: Department of Pharmacy, Graduate Program in Pharmaceutical Sciences, State University of Maringá, Avenida Colombo 5790, 87.020-900 Maringá, PR, Brazil.
authors.forename: Arildo José Braz
authors.initials: AJ
authors.lastname: de Oliveira
authors.affiliation: Department of Pharmacy, Graduate Program in Pharmaceutical Sciences, State University of Maringá, Avenida Colombo 5790, 87.020-900 Maringá, PR, Brazil. Electronic address: racgoncalves@uem.br.
authors.forename: Regina Aparecida Correia
authors.initials: RA
authors.lastname: Gonçalves
mesh_terms: D050842:1-Pyrroline-5-Carboxylate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D018533:Biomass; D000431:Ethanol / Q000378:metabolism; D005838:Genotype; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism; D030821:Plants, Genetically Modified; D011392:Proline / Q000096:biosynthesis* / Q000378:metabolism; D031786:Saccharum / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism; D000070658:Vigna / Q000201:enzymology / Q000235:genetics; D014867:Water / Q000737:chemistry
chemical_list: D009097:Multienzyme Complexes; D010940:Plant Proteins; D014867:Water; D000431:Ethanol; D013395:Sucrose; D011392:Proline; D050842:1-Pyrroline-5-Carboxylate Dehydrogenase
keywords: Biochemical composition; NMR; Proline; Sucrose; Transgenic sugarcane