title: Potential of wheat bran to promote indigenous microbial enhanced oil recovery.
abstract: Microbial enhanced oil recovery (MEOR) is an emerging oil extraction technology that utilizes microorganisms to facilitate recovery of crude oil in depleted petroleum reservoirs. In the present study, effects of wheat bran utilization were investigated on stimulation of indigenous MEOR. Biostimulation conditions were optimized with the response surface methodology. The co-application of wheat bran with KNO3and NH4H2PO4significantly promoted indigenous MEOR (IMEOR) and exhibited sequential aerobic (O-), facultative (An-) and anaerobic (A0-) metabolic stages. The surface tension of fermented broth decreased by approximately 35%, and the crude oil was highly emulsified. Microbial community structure varied largely among and in different IMEOR metabolic stages. Pseudomonas sp., Citrobacter sp., and uncultured Burkholderia sp. dominated the O-, An- and early A0-stages. Bacillus sp., Achromobacter sp., Rhizobiales sp., Alcaligenes sp. and Clostridium sp. dominated the later A0-stage. This study illustrated occurrences of microbial community succession driven by wheat bran stimulation and its industrial potential.
authors.affiliation: State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing, 102249, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Zhan
authors.affiliation: State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing, 102249, China.
authors.forename: Qinghong
authors.initials: Q
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing, 102249, China.|Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI, 96822, USA.
authors.forename: Chunmao
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Agro-Food Resources, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju, 55365, Republic of Korea.
authors.forename: Jung Bong
authors.initials: JB
authors.lastname: Kim
authors.affiliation: State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing, 102249, China.
authors.forename: Hongdan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Hawaii Natural Energy Institute, University of Hawaii at Manoa, Honolulu, HI, 96822, USA.
authors.forename: Brandon A
authors.initials: BA
authors.lastname: Yoza
authors.affiliation: Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI, 96822, USA. qingl@hawaii.edu.
authors.forename: Qing X
authors.identifier: 0000-0003-4589-2869
authors.initials: QX
authors.lastname: Li
mesh_terms: D001419:Bacteria / Q000302:isolation & purification; D004043:Dietary Fiber; D005285:Fermentation; D010578:Petroleum / Q000378:metabolism / Q000382:microbiology; D014908:Triticum
chemical_list: D004043:Dietary Fiber; D010578:Petroleum
keywords: Biostimulation; Indigenous microbial enhanced oil recovery; Response surface methodology; Wheat bran