title: Experimental Guillain-Barre syndrome induced by immunization with gangliosides: Keyhole limpet hemocyanin is required for disease triggering.
abstract: An experimental model of Guillain-Barré Syndrome has been established in recent years. Rabbits develop disease upon immunization with a single dose of an emulsion containing bovine brain gangliosides, KLH and complete Freund's adjuvant. Within a period of four to ten weeks after immunization, they began to produce anti-ganglioside IgG-antibodies first, and to show clinical signs of neuropathy afterwards. In addition to gangliosides, KLH is a requirement for antibody production and disease triggering. Although KLH is commonly used as an immunological carrier protein, an anti-KLH-specific immune response was necessary for induction of both events. KLH is a glycoprotein carrying most of the immunogenicity in its glycan moiety. Between 20% to 80% of anti-ganglioside IgG-antibodies present in sick rabbit sera cross-reacted with KLH, indicating that both immune responses are related. The terminal Gal-ß(1,3)-GalNAc glycan (present in gangliosides and KLH) is proposed as "key" antigenic determinant involved in inducing the anti-ganglioside immune response. These results are discussed in the context of the "binding site drift" hypothesis.
authors.affiliation: CIQUIBIC, CONICET, Departamento de Química Biológica "Dr. Ranwel Caputto", Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
authors.forename: Samanta C
authors.initials: SC
authors.lastname: Funes
authors.affiliation: CIQUIBIC, CONICET, Departamento de Química Biológica "Dr. Ranwel Caputto", Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
authors.forename: María Eugenia
authors.initials: ME
authors.lastname: Chiari
authors.affiliation: CIQUIBIC, CONICET, Departamento de Química Biológica "Dr. Ranwel Caputto", Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
authors.forename: Romina
authors.initials: R
authors.lastname: Comín
authors.affiliation: CIQUIBIC, CONICET, Departamento de Química Biológica "Dr. Ranwel Caputto", Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
authors.forename: Fernando J
authors.initials: FJ
authors.lastname: Irazoqui
authors.affiliation: CIQUIBIC, CONICET, Departamento de Química Biológica "Dr. Ranwel Caputto", Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina. Electronic address: gnores@fcq.unc.edu.ar.
authors.forename: Gustavo A
authors.initials: GA
authors.lastname: Nores
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage* / Q000494:pharmacology; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D004195:Disease Models, Animal; D020275:Guillain-Barre Syndrome / Q000139:chemically induced / Q000276:immunology / Q000473:pathology; D006433:Hemocyanins / Q000009:adverse effects* / Q000494:pharmacology; D006801:Humans; D007114:Immunization / Q000009:adverse effects*; D018448:Models, Immunological; D011817:Rabbits
chemical_list: D000276:Adjuvants, Immunologic; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin
keywords: Anti-ganglioside antibodies; Binding site drift; Experimental model; Guillain-Barré syndrome; KLH