title: Characterisation and genome sequence of the lytic Acinetobacter baumannii bacteriophage vB_AbaS_Loki.
abstract: Acinetobacter baumannii has emerged as an important nosocomial pathogen in healthcare and community settings. While over 100 of Acinetobacter phages have been described in the literature, relatively few have been sequenced. This work describes the characterisation and genome annotation of a new lytic Acinetobacter siphovirus, vB_AbaS_Loki, isolated from activated sewage sludge. Sequencing revealed that Loki encapsulates a 41,308 bp genome, encoding 51 predicted open reading frames. Loki is most closely related to Acinetobacter phage IME_AB3 and more distantly related to Burkholderia phage KL1, Paracoccus phage vB_PmaS_IMEP1 and Pseudomonas phages vB_Pae_Kakheti25, vB_PaeS_SCH_Ab26 and PA73. Loki is characterised by a narrow host range, among the 40 Acinetobacter isolates tested, productive infection was only observed for the propagating host, A. baumannii ATCC 17978. Plaque formation was found to be dependent upon the presence of Ca2+ ions and adsorption to host cells was abolished upon incubation with a mutant of ATCC 17978 encoding a premature stop codon in lpxA. The complete genome sequence of vB_AbaS_Loki was deposited in the European Nucleotide Archive (ENA) under the accession number LN890663.
authors.affiliation: Centre for Research in Biosciences, Department of Applied Sciences, Faculty of Health and Applied Sciences, University of the West of England, Coldharbour Lane, Bristol, United Kingdom.
authors.forename: Dann
authors.identifier: http://orcid.org/0000-0002-0249-4513
authors.initials: D
authors.lastname: Turner
authors.affiliation: National Infections Service, Public Health England, Porton Down, Salisbury, Wiltshire, United Kingdom.
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Wand
authors.affiliation: Laboratory of Applied Biotechnology, Department of Applied Biosciences, Ghent University, Ghent, Belgium.|Laboratory of Gene Technology, Biosystems Department, KU Leuven, Heverlee, Belgium.
authors.forename: Yves
authors.initials: Y
authors.lastname: Briers
authors.affiliation: Laboratory of Gene Technology, Biosystems Department, KU Leuven, Heverlee, Belgium.
authors.forename: Rob
authors.initials: R
authors.lastname: Lavigne
authors.affiliation: National Infections Service, Public Health England, Porton Down, Salisbury, Wiltshire, United Kingdom.
authors.forename: J Mark
authors.initials: JM
authors.lastname: Sutton
authors.affiliation: Centre for Research in Biosciences, Department of Applied Sciences, Faculty of Health and Applied Sciences, University of the West of England, Coldharbour Lane, Bristol, United Kingdom.
authors.forename: Darren M
authors.initials: DM
authors.lastname: Reynolds
mesh_terms: D000151:Acinetobacter Infections / Q000382:microbiology*; D040981:Acinetobacter baumannii / Q000302:isolation & purification / Q000821:virology*; D001435:Bacteriophages / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D004279:DNA, Viral / Q000235:genetics*; D016679:Genome, Viral; D006801:Humans; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000235:genetics*; D053585:Virus Attachment
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins