title: Proteome Characterization of a Chromatin Locus Using the Proteomics of Isolated Chromatin Segments Approach.
abstract: The biological functions of given genomic regions are ruled by the local chromatin composition. The Proteomics of Isolated Chromatin segments approach (PICh) is a powerful and unbiased method to analyze the composition of chosen chromatin segments, provided they are abundant (repeated) or that the organism studied has a small genome. PICh can be used to identify novel and unexpected regulatory factors, or when combined with quantitative mass spectrometric approaches, to characterize the function of a defined factor at the chosen locus, by quantifying composition changes at the locus upon removal/addition of that factor.
authors.affiliation: INSERM AVENIR, Institute of Human Genetics CNRS UPR1142, 141 rue de la Cardonille, 34000, Montpellier, France.
authors.forename: Sophie L
authors.initials: SL
authors.lastname: Kan
authors.affiliation: INSERM AVENIR, Institute of Human Genetics CNRS UPR1142, 141 rue de la Cardonille, 34000, Montpellier, France.
authors.forename: Nehmé
authors.initials: N
authors.lastname: Saksouk
authors.affiliation: INSERM AVENIR, Institute of Human Genetics CNRS UPR1142, 141 rue de la Cardonille, 34000, Montpellier, France. jerome.dejardin@igh.cnrs.fr.
authors.forename: Jérome
authors.initials: J
authors.lastname: Déjardin
mesh_terms: D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002843:Chromatin / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D047369:Chromatin Immunoprecipitation / Q000379:methods; D006570:Heterochromatin / Q000235:genetics / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry; D009693:Nucleic Acid Hybridization / Q000379:methods; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid; D016615:Telomere; D057188:Workflow
chemical_list: D002843:Chromatin; D006570:Heterochromatin; D011506:Proteins; D020543:Proteome
keywords: Chromatin; Heterochromatin; Protein Purification; Proteomics; Repeated Sequences; Telomeres