title: Copy number analysis identifies tumor suppressive lncRNAs in human osteosarcoma.
abstract: Osteosarcoma (OS) has a high degree of chromosomal instability and total copy number (CN) changes. We examined 58 human OS samples including 40 primary tumors, 11 explants, and 7 cell lines using single nucleotide polymorphism (SNP) arrays, and revealed that 70% of the samples had one or more recurrent CN-neutral loss of heterozygosity (CNN‑LOH) also known as uniparental disomy (UPD). Importantly, 17% of the samples showed prominent homozygous deletion of 3q13.31, suggesting its role in tumorigenesis. We identified and characterized two novel lncRNAs, LOC285194 and BC040587, within this genomic locus, strongly suggesting their tumor suppressor activity. Frequent deletions and UPD suggest that OS often has mutant or non-expressed tumor suppressor genes including two lncRNAs.
authors.affiliation: Division of Hematology and Oncology, Cedars-Sinai Medical Center, UCLA School of Medicine, Los Angeles, CA 90048, USA.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Xie
authors.affiliation: Division of Hematology and Oncology, Cedars-Sinai Medical Center, UCLA School of Medicine, Los Angeles, CA 90048, USA.
authors.forename: Dechen
authors.initials: D
authors.lastname: Lin
authors.affiliation: Division of Hematology and Oncology, Cedars-Sinai Medical Center, UCLA School of Medicine, Los Angeles, CA 90048, USA.
authors.forename: Dhong Hyun Tony
authors.initials: DH
authors.lastname: Lee
authors.affiliation: Center for Molecular Medicine, University of Connecticut Health Center, Farmington, CT 06030-3101, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Akunowicz
authors.affiliation: Center for Molecular Medicine, University of Connecticut Health Center, Farmington, CT 06030-3101, USA.
authors.forename: Marc
authors.initials: M
authors.lastname: Hansen
authors.affiliation: Division of Hematology and Oncology, Cedars-Sinai Medical Center, UCLA School of Medicine, Los Angeles, CA 90048, USA.
authors.forename: Carl
authors.initials: C
authors.lastname: Miller
authors.affiliation: Pathology and Tumor Biology, Kyoto University, Kyoto 606-8315, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Sanada
authors.affiliation: Pathology and Tumor Biology, Kyoto University, Kyoto 606-8315, Japan.
authors.forename: Motohiro
authors.initials: M
authors.lastname: Kato
authors.affiliation: Division of Hematology and Oncology, Cedars-Sinai Medical Center, UCLA School of Medicine, Los Angeles, CA 90048, USA.
authors.forename: Tadayuki
authors.initials: T
authors.lastname: Akagi
authors.affiliation: Division of Hematology and Oncology, Cedars-Sinai Medical Center, UCLA School of Medicine, Los Angeles, CA 90048, USA.
authors.forename: Norihiko
authors.initials: N
authors.lastname: Kawamata
authors.affiliation: Pathology and Tumor Biology, Kyoto University, Kyoto 606-8315, Japan.
authors.forename: Seishi
authors.initials: S
authors.lastname: Ogawa
authors.affiliation: Division of Hematology and Oncology, Cedars-Sinai Medical Center, UCLA School of Medicine, Los Angeles, CA 90048, USA.
authors.forename: H Phillip
authors.initials: HP
authors.lastname: Koeffler
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D056915:DNA Copy Number Variations / Q000235:genetics*; D018628:Gene Dosage / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D020411:Oligonucleotide Array Sequence Analysis; D012516:Osteosarcoma / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D062085:RNA, Long Noncoding / Q000235:genetics*; D014183:Transplantation, Heterologous; D024182:Uniparental Disomy / Q000235:genetics
chemical_list: D062085:RNA, Long Noncoding