title: Simultaneous quantification of antofloxacin and its major metabolite in human urine by HPLC-MS/MS, and its application to a pharmacokinetic study.
abstract: This study presents a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the simultaneous determination of antofloxacinin and its main metabolite - N-demethylated metabolite (N-DM) - in human urine. Ornidazole was used as the internal standard. This was a clinical urine recovery study, in which 10 healthy Chinese volunteers were intravenously administered a single 200 mg dose of antofloxacin hydrochloride. Compounds were extracted by albumen precipitation, after which samples were isocratically eluted using a Poroshell 120 SB-C18column, and were analysed using HPLC-MS/MS under electronic spray ionization positive ion mode. The method was successfully applied in a urine pharmacokinetic study of antofloxacinin, with a detection range of 0.02/0.01 to 200/100 μg/mL (for antofioxacin/N-DM).The average percentages of antofioxacin/N-DM measured in urinary excretion frp, 10 volunteers were 54.9 ± 5.7/8.2 ± 2.5% in 120 h duration.
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing, China.
authors.forename: Yunsu
authors.initials: Y
authors.lastname: Ma
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing, China.
authors.forename: Qianru
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital with Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Hongwen
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital with Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Xie
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital with Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital with Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Mao
authors.initials: M
authors.lastname: Huang
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital with Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital with Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital with Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Libin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital with Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Luning
authors.initials: L
authors.lastname: Sun
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing, China.|Department of Pharmacy, The First Affiliated Hospital with Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Ning
authors.identifier: http://orcid.org/0000-0001-6016-1111
authors.initials: N
authors.lastname: Ou
mesh_terms: D000293:Adolescent; D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005260:Female; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D015242:Ofloxacin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics / Q000652:urine; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D055815:Young Adult
chemical_list: C522674:antofloxacin; D015242:Ofloxacin
keywords: LC-MS/MS; N-demethylated metabolites of antofloxacin; antofloxacin; urinary excretion; urine