title: Filamentous hemagglutinin adhesin FhaB limitsA.baumanniibiofilm formation.
abstract: Increased resistance and survival, as well as immune evasion, play a significant role in the pathogenicity ofAcinetobacter baumannii. Here, we report on the adhesion of the bacterium to epithelial cells and formation of biofilm on abiotic surfaces. We identificed autotransporter (AT) genes that encode homologues (Fha-like) of the two-partner secretion system (TPS) inA.baumannii(ATCC19606) which we designate as FhaB (exoprotein) and FhaC (transporter) and demonstrate that these novel genes, are under the control of distinct regulatable promoters within either the same (FhaBC) or two distinct (FhaB and FhaC) cells. The expression of this gene in outer membrane protein (OM) showed them to be deficient in the adherence to A546 cells. FhaB is involved in hydrophobicity ofA. baumanniiATCC19606 while FhaBC is associated with bioﬁlm formation. The vaccinogenic potential of FHA-like proteins offers use of these targets as novel therapeutic strategies to limitA.baumanniiassociated morbidity and mortality.
authors.affiliation: Department of Biotechnology, Semnan University, Semnan, Iran.
authors.forename: Shakiba
authors.initials: S
authors.lastname: Darwish Alipour Astaneh
authors.affiliation: Department of Biology, Shahed University, Opposite Imam Khomeini's Shrine, Tehran-Qom Express Way, Tehran, Iran, rasooli@shahed.ac.ir.
authors.forename: Iraj
authors.initials: I
authors.lastname: Rasooli
authors.affiliation: Department of Biology, Shahed University, Opposite Imam Khomeini's Shrine, Tehran-Qom Express Way, Tehran, Iran, 3Molecular Microbiology Research Center, Shahed University, Tehran, Iran.
authors.forename: Seyed Latif
authors.initials: SL
authors.lastname: Mousavi Gargari
mesh_terms: D040981:Acinetobacter baumannii / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D018829:Adhesins, Bacterial / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D000595:Amino Acid Sequence; D001422:Bacterial Adhesion / Q000235:genetics; D018441:Biofilms; D003001:Cloning, Molecular; D019295:Computational Biology; D017124:Conserved Sequence; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D057927:Hydrophobic and Hydrophilic Interactions; D011993:Recombinant Fusion Proteins; D016415:Sequence Alignment
chemical_list: D018829:Adhesins, Bacterial; D011993:Recombinant Fusion Proteins