title: [MALDI-TOF mass spectrometry: Evaluation of the preanalytical phase for identification of molds].
abstract: In order to optimize the identification of molds with MALDI-TOF MS, three protein extraction-methodologies were evaluated against 44 isolates: water extraction (WE), zirconium extraction (ZE) and the provider's recommended method (PRM). Two data bases were compared, Bruker (BK) and Bruker+National Institutes of Health. Considering both databases, results were respectively as follows: correct identification (CI) at gender level, 10 and 16 by WE; 27 and 32 by ZE and 18 and 23 by PRM; CI at species level, 5 and 7 by WE; 17 and 20 by ZE and 11 and 14 by PRM; non-reliable identification, 18 and 12 by WE; 9 and 4 by ZE and by PRM. No peaks were observed in 16 by WE, 8 by ZE and 17 by PRM. ZE showed the best perfomance (p<0.05).
authors.affiliation: Laboratorio de Microbiología, Hospital Alemán, Ciudad Autónoma de Buenos Aires, Argentina. Electronic address: ivanam27@gmail.com.
authors.forename: Ivana
authors.initials: I
authors.lastname: Maldonado
authors.affiliation: Laboratorio de Microbiología, Hospital Alemán, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Dolores
authors.initials: D
authors.lastname: García Ramírez
authors.affiliation: Laboratorio de Microbiología, Hospital Alemán, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Pablo
authors.initials: P
authors.lastname: Striebeck
authors.affiliation: Laboratorio de Microbiología, Hospital Alemán, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Lafage
authors.affiliation: Laboratorio de Microbiología, Hospital Alemán, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Liliana
authors.initials: L
authors.lastname: Fernández Canigia
mesh_terms: D016208:Databases, Factual; D005658:Fungi / Q000302:isolation & purification; D000073623:Pre-Analytical Phase; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
keywords: Espectrometría de masas; Hongos miceliales; Mass spectrometry; Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; Molds