title: Preservation of Specific Protein Signaling States Using Heat Based Stabilizor System.
abstract: The ability to adequately measure the phosphorylation state of a protein has major biological as well as clinical relevance. Due to its variable nature, reversible protein phosphorylations are sensitive to changes in the tissue environment. StabilizorTMT1 is a system for rapid inactivation of enzymatic activity in biological samples. Enzyme inactivation is accomplished using thermal denaturation in a rapid, homogeneous, and reproducible fashion without the need of added chemical inhibitors. Using pCREB(Ser133) as a model system, the applicability of the Stabilizor system to preserve a rapidly lost phosphorylation is shown.
authors.affiliation: Denator AB, Uppsala, Sweden. mats@denator.com.
authors.forename: Mats
authors.initials: M
authors.lastname: Borén
mesh_terms: D000818:Animals; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D006358:Hot Temperature; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D010766:Phosphorylation; D011309:Preservation, Biological / Q000379:methods*; D011485:Protein Binding; D055550:Protein Stability; D011506:Proteins / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D011506:Proteins
keywords: CREB; Denator; Immunohistochemistry; Phosphorylation; Phosphospecific antibody; Postmortem change; Stabilizor