title: An In Silico Identification of Common Putative Vaccine Candidates against Treponema pallidum: A Reverse Vaccinology and Subtractive Genomics Based Approach.
abstract: Sexually transmitted infections (STIs) are caused by a wide variety of bacteria, viruses, and parasites that are transmitted from one person to another primarily by vaginal, anal, or oral sexual contact. Syphilis is a serious disease caused by a sexually transmitted infection. Syphilis is caused by the bacteriumTreponema pallidumsubspeciespallidum.Treponema pallidum(T. pallidum) is a motile, gram-negative spirochete, which can be transmitted both sexually and from mother to child, and can invade virtually any organ or structure in the human body. The current worldwide prevalence of syphilis emphasizes the need for continued preventive measures and strategies. Unfortunately, effective measures are limited. In this study, we focus on the identification of vaccine targets and putative drugs against syphilis disease using reverse vaccinology and subtractive genomics. We compared 13 strains ofT. pallidumusingT. pallidumNichols as the reference genome. Using an in silicoapproach, four pathogenic islands were detected in the genome ofT. pallidumNichols. We identified 15 putative antigenic proteins and sixdrug targets through reverse vaccinology and subtractive genomics, respectively, which can be used as candidate therapeutic targets in the future.
authors.affiliation: Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil. arunjaiswal1411@gmail.com.|Department of Immunology, Microbiology and Parasitology, Institute of Biological Sciences and Natural Sciences, Federal University of Triângulo Mineiro (UFTM), Uberaba 38025-180, MG, Brazil. arunjaiswal1411@gmail.com.
authors.forename: Arun
authors.initials: A
authors.lastname: Kumar Jaiswal
authors.affiliation: Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil. sandip_sbtbi@yahoo.com.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Tiwari
authors.affiliation: Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil. syedbabar.jamal@gmail.com.
authors.forename: Syed Babar
authors.initials: SB
authors.lastname: Jamal
authors.affiliation: Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil. dr.barh@gmail.com.|Centre for Genomics and Applied Gene Technology, Institute of Integrative Omics and Applied Biotechnology (IIOAB), Nonakuri, Purba Medinipur, West Bengal 721137, India. dr.barh@gmail.com.
authors.forename: Debmalya
authors.initials: D
authors.lastname: Barh
authors.affiliation: Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil. vascoariston@gmail.com.
authors.forename: Vasco
authors.initials: V
authors.lastname: Azevedo
authors.affiliation: Department of Immunology, Microbiology and Parasitology, Institute of Biological Sciences and Natural Sciences, Federal University of Triângulo Mineiro (UFTM), Uberaba 38025-180, MG, Brazil. siomars@gmail.com.
authors.forename: Siomar C
authors.initials: SC
authors.lastname: Soares
mesh_terms: D000942:Antigens, Bacterial / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology*; D019295:Computational Biology / Q000379:methods; D003198:Computer Simulation; D018604:Epitope Mapping / Q000379:methods; D016680:Genome, Bacterial; D044404:Genomic Islands; D023281:Genomics / Q000379:methods; D008958:Models, Molecular; D013329:Structure-Activity Relationship; D013587:Syphilis / Q000517:prevention & control*; D014210:Treponema pallidum / Q000235:genetics / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D001428:Bacterial Vaccines
keywords: drug target; sexually transmitted infections (STIs); vaccine target