title: Anti-nociceptive Effect of 7-methoxy Coumarin fromEupatorium Triplinerve vahl(Asteraceae).
abstract: OBJECTIVE
  To evaluate the anti-nociceptive activity of 7-methoxy coumarin isolated from ethyl acetate fraction of the alcoholic extract ofEupatorium triplinerve Vahl.
  
  
  METHODS
  The shade dried leaves ofE. triplinervewere extracted with ethyl alcohol and the extract was condensed. This extract was fractionated with n-hexane, ethyl acetate, and n-butanol. The ethyl acetate fraction was subjected to column chromatography which yielded a crystalline compound-A, which was investigated for spectral characteristics.
  The isolated compound-A was subjected to behavioral studies and anti-nociceptive evaluation in mice by acetic acid induced writhing and formalin induced nociception.
  
  
  RESULTS
  The spectral studies indicated that the structure of compound-A complies with 7- methoxy coumarin. Pre-treatment with 7-methoxy coumarin reduced the number of abdominal constrictions in mice and decreased the time spent in paw licking and biting response in formalin assay. There were no significant behavioral changes.
  
  
  CONCLUSIONS
  A dose dependent anti-nociceptive action of 7- methoxy coumarin was revealed by the present experiments which support the traditional use ofE. triplinervein pain and inflammatory disorders.
  
  
  CONCLUSIONS
  Bio-guided fractionation of alcoholic extract ofE. triplinerveyielded 7-methoxy coumarin.7-methoxy coumarin was evaluated for its anti-nociceptive potential by acetic acid induced writhing and formalin induced nociception assays.7-methoxy coumarin exhibited significant inhibition of acetic acid induced writhing response and the second phase of formalin nociception.The anti-nociceptive action of 7-methoxy coumarin revealed by the present experiments supports the traditional use of E. triplinerve in pain and inflammatory disorders.Abbreviation used:TLC-Thin layer chromatography, Kg-kilogram, g-gram, TXB2-Thromboxane B2, UV-Ultraviolet, IgE-Immunoglobulin E, s.c-subcutaneous, p.o-oral route.
authors.affiliation: Department of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Vels University, Pallavaram, Chennai, India.
authors.forename: Binoy Varghese
authors.initials: BV
authors.lastname: Cheriyan
authors.affiliation: Department of Pharmacology, Meenakshi Medical College and Research Institute, Enathur, Kanchipuram, India.
authors.forename: Parimala
authors.initials: P
authors.lastname: Kadhirvelu
authors.affiliation: Department of Pharmacology, MNR Medical College and Hospital, Fasalwadi, Sangareddy, Medak, Telangana, India.
authors.forename: Jagan
authors.initials: J
authors.lastname: Nadipelly
authors.affiliation: Department of Pharmacology, Meenakshi Medical College and Research Institute, Enathur, Kanchipuram, India.
authors.forename: Jaikumar
authors.initials: J
authors.lastname: Shanmugasundaram
authors.affiliation: Department of Pharmacology, Mamata Medical College and Hospital, Rotary Nagar, Khammam, Telangana, India.
authors.forename: Vijaykumar
authors.initials: V
authors.lastname: Sayeli
authors.affiliation: Department of Pharmacology, Meenakshi Medical College and Research Institute, Enathur, Kanchipuram, India.
authors.forename: Viswanathan
authors.initials: V
authors.lastname: Subramanian
keywords: 7-methoxy coumarin; anti-nociception; ayapanin; eupatorium triplinerve (E. triplinerve); herniarin