title: Improving Proteome Coverage and Sample Recovery with Enhanced FASP (eFASP) for Quantitative Proteomic Experiments.
abstract: Enhanced Filter Aided Sample Preparation (eFASP) incorporates plastics passivation and digestion-enhancing surfactants into the traditional FASP workflow to reduce sample loss and increase hydrophobic protein representation in qualitative and quantitative proteomics experiments. Resulting protein digests are free of contaminants and can be analyzed directly by LC-MS.
authors.affiliation: Department of Chemistry and Biochemistry, University of California-Los Angeles, Los Angeles, CA, 90095, USA.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Erde
authors.affiliation: Department of Biological Chemistry, University of California-Los Angeles, Los Angeles, CA, 90095, USA.
authors.forename: Rachel R Ogorzalek
authors.initials: RR
authors.lastname: Loo
authors.affiliation: Department of Chemistry and Biochemistry, University of California-Los Angeles, Los Angeles, CA, 90095, USA. jloo@chem.ucla.edu.|Department of Biological Chemistry, University of California-Los Angeles, Los Angeles, CA, 90095, USA. jloo@chem.ucla.edu.
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Loo
mesh_terms: D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D020543:Proteome; D040901:Proteomics / Q000379:methods*
chemical_list: D011506:Proteins; D020543:Proteome
keywords: Ammonium deoxycholate; Detergent; Enhanced filter aided sample preparation; Quantitative proteomics