title: Mitochondrial Ferritin Deletion Exacerbatesβ-Amyloid-Induced Neurotoxicity in Mice.
abstract: Mitochondrial ferritin (FtMt) is a mitochondrial iron storage protein which protects mitochondria from iron-induced oxidative damage. Our previous studies indicate that FtMt attenuatesβ-amyloid- and 6-hydroxydopamine-induced neurotoxicity in SH-SY5Y cells. To explore the protective effects of FtMt onβ-amyloid-induced memory impairment and neuronal apoptosis and the mechanisms involved, 10-month-old wild-type andFtmtknockout mice were infused intracerebroventricularly (ICV) with Aβ25-35to establish an Alzheimer's disease model. Knockout ofFtmtsignificantly exacerbated Aβ25-35-induced learning and memory impairment. The Bcl-2/Bax ratio in mouse hippocampi was decreased and the levels of cleaved caspase-3 and PARP were increased. The number of neuronal cells undergoing apoptosis in the hippocampus was also increased inFtmtknockout mice. In addition, the levels of L-ferritin and FPN1 in the hippocampus were raised, and the expression of TfR1 was decreased. Increased MDA levels were also detected inFtmtknockout mice treated with Aβ25-35. In conclusion, this study demonstrated that the neurological impairment induced by Aβ25-35was exacerbated inFtmtknockout mice and that this may relate to increased levels of oxidative stress.
authors.affiliation: Laboratory of Molecular Iron Metabolism, The Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Science, Hebei Normal University, Shijiazhuang, Hebei 050024, China.
authors.forename: Peina
authors.initials: P
authors.lastname: Wang
authors.affiliation: Laboratory of Molecular Iron Metabolism, The Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Science, Hebei Normal University, Shijiazhuang, Hebei 050024, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Laboratory of Molecular Iron Metabolism, The Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Science, Hebei Normal University, Shijiazhuang, Hebei 050024, China.
authors.forename: Wenyue
authors.initials: W
authors.lastname: Wu
authors.affiliation: Laboratory of Molecular Iron Metabolism, The Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Science, Hebei Normal University, Shijiazhuang, Hebei 050024, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Li
authors.affiliation: Laboratory of Molecular Iron Metabolism, The Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Science, Hebei Normal University, Shijiazhuang, Hebei 050024, China.
authors.forename: Yuetong
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Laboratory of Molecular Iron Metabolism, The Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Science, Hebei Normal University, Shijiazhuang, Hebei 050024, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Yu
authors.affiliation: Laboratory of Molecular Iron Metabolism, The Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Science, Hebei Normal University, Shijiazhuang, Hebei 050024, China.
authors.forename: Guofen
authors.initials: G
authors.lastname: Gao
authors.affiliation: Laboratory of Molecular Iron Metabolism, The Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Science, Hebei Normal University, Shijiazhuang, Hebei 050024, China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Shi
authors.affiliation: Laboratory of Molecular Iron Metabolism, The Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Science, Hebei Normal University, Shijiazhuang, Hebei 050024, China.
authors.forename: Baolu
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Laboratory of Molecular Iron Metabolism, The Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Science, Hebei Normal University, Shijiazhuang, Hebei 050024, China.
authors.forename: Yan-Zhong
authors.identifier: 0000-0002-6386-9649
authors.initials: YZ
authors.lastname: Chang
mesh_terms: D000544:Alzheimer Disease; D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D015153:Blotting, Western; D004195:Disease Models, Animal; D005293:Ferritins / Q000172:deficiency* / Q000235:genetics; D018782:Maze Learning / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D024101:Mitochondrial Proteins / Q000172:deficiency* / Q000235:genetics; D018384:Oxidative Stress / Q000502:physiology*; D010446:Peptide Fragments / Q000633:toxicity*
chemical_list: D016229:Amyloid beta-Peptides; D024101:Mitochondrial Proteins; D010446:Peptide Fragments; C074636:amyloid beta-protein (25-35); D005293:Ferritins