title: Comparative analysis of the immunogenicity of monovalent and multivalent rotavirus immunogens.
abstract: The strategies for developing rotavirus (RV) vaccines have always been controversial. At present, both the monovalent RV vaccine and the multivalent RV vaccine have displayed excellent safety and efficacy against RV infection and shown cross-reactive immunity, which laid the question whether the multivalent RV vaccine could be replaced by the monovalent RV vaccine. In this study, we focused on comparing the immunogenicity (serum neutralization activity and protection against homotypic and heterotypic RVs' challenge) of individual standard RV strains (monovalent RV immunogens) and different combinations of them (multivalent RV immunogens). In result, RV immunogens showed general immunogenicity and heterotypic reaction but the multivalent RV immunogens exhibited greater serum neutralization activity and stronger heterotypic reaction than the monovalent RV immunogens (P<0.05). As to the protection, the multivalent RV immunogens also revealed more rapid and stronger protection against homotypic and heterotypic RVs' challenge than the monovalent RV immunogens. The results demonstrated that both the monovalent and multivalent RV immunogens exhibited high immunogenicity, but the monovalent RV immunogens could not provide enough neutralization antibodies to protect MA104 cells against the infection with heterotypic RV strains and timely protection against homotypic and heterotypic RVs, so the multivalent RV vaccine could not be replaced by the monovalent RV vaccine.
authors.affiliation: Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan Province, the People's Republic of China.|School of Basic Medicine, Kunming Medical University, Kunming, Yunnan Province, the People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Mi
authors.affiliation: School of Basic Medicine, Kunming Medical University, Kunming, Yunnan Province, the People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Ou
authors.affiliation: Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan Province, the People's Republic of China.
authors.forename: Lili
authors.initials: L
authors.lastname: Guo
authors.affiliation: Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan Province, the People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ye
authors.affiliation: Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan Province, the People's Republic of China.
authors.forename: Jinyuan
authors.initials: J
authors.lastname: Wu
authors.affiliation: Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan Province, the People's Republic of China.
authors.forename: Shan
authors.initials: S
authors.lastname: Yi
authors.affiliation: Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan Province, the People's Republic of China.
authors.forename: Xianglian
authors.initials: X
authors.lastname: Niu
authors.affiliation: Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan Province, the People's Republic of China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Sun
authors.affiliation: Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan Province, the People's Republic of China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Li
authors.affiliation: Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan Province, the People's Republic of China.
authors.forename: Maosheng
authors.initials: M
authors.lastname: Sun
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000145:classification* / Q000276:immunology*; D005260:Female; D051379:Mice; D008813:Mice, Inbred ICR; D012401:Rotavirus / Q000276:immunology*; D012400:Rotavirus Infections / Q000276:immunology* / Q000517:prevention & control; D022243:Rotavirus Vaccines / Q000008:administration & dosage / Q000276:immunology*; D014611:Vaccination
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000956:Antigens, Viral; D022243:Rotavirus Vaccines