title: Isolation and Structure Elucidation of Glucosylated Colchicinoids from the Seeds of Gloriosa superba by LC-DAD-SPE-NMR.
abstract: Four new colchicinoids were isolated from the seeds of Gloriosa superba together with the known compounds colchicoside (4) and 3-de-O-methylcolchicine-3-O-β-d-glucopyranosyl-(1→4)-3-O-β-d-glucopyranoside (6), by means of conventional column chromatography and LC-DAD-SPE-NMR. The new compounds were identified as N-deacetyl-N-formyl-3-de-O-methylcolchicine-3-O-β-d-glucopyranoside (1), 3-de-O-methylcolchicine-3-O-β-d-glucopyranosyl-(1→6)-3-O-β-d-glucopyranoside (2), N-deacetyl-N-formyl-3-de-O-methylcolchicine-3-O-β-d-glucopyranosyl-(1→6)-3-O-β-d-glucopyranoside (3), and 3-de-O-methylcolchicine-3-O-β-d-glucopyranosyl-(1→3)-3-O-β-d-glucopyranoside (5). The structure elucidation was performed by means of NMR (COSY, HSQC, and HMBC), HRESIMS/MS, and GCMS data analysis.
authors.affiliation: Natural Products & Food Research and Analysis (NatuRA), Department of Pharmaceutical Sciences, University of Antwerp , Universiteitsplein 1, 2610 Antwerp, Belgium.
authors.forename: Yancho
authors.identifier: 0000-0001-5830-4697
authors.initials: Y
authors.lastname: Zarev
authors.affiliation: Natural Products & Food Research and Analysis (NatuRA), Department of Pharmaceutical Sciences, University of Antwerp , Universiteitsplein 1, 2610 Antwerp, Belgium.
authors.forename: Kenn
authors.initials: K
authors.lastname: Foubert
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Medical University-Sofia , Street Dunav 2, 1000 Sofia, Bulgaria.
authors.forename: Iliana
authors.initials: I
authors.lastname: Ionkova
authors.affiliation: Natural Products & Food Research and Analysis (NatuRA), Department of Pharmaceutical Sciences, University of Antwerp , Universiteitsplein 1, 2610 Antwerp, Belgium.
authors.forename: Sandra
authors.initials: S
authors.lastname: Apers
authors.affiliation: Natural Products & Food Research and Analysis (NatuRA), Department of Pharmaceutical Sciences, University of Antwerp , Universiteitsplein 1, 2610 Antwerp, Belgium.
authors.forename: Luc
authors.initials: L
authors.lastname: Pieters
mesh_terms: D000070437:Colchicaceae / Q000737:chemistry; D003078:Colchicine / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification; D007194:India; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification; D012639:Seeds / Q000737:chemistry
chemical_list: D003078:Colchicine; D006027:Glycosides; D012503:Saponins