title: Genetic Polymorphism of Aedes albopictus Population Inferred From ND5 Gene Variabilities In Subang Jaya, Malaysia.
abstract: This study was performed to establish the genetic variability of Aedes albopictus within Subang Jaya, Selangor, Malaysia, by using the nicotinamide adenine dinucleotide dehydrogenase 5 subunit (ND5) mitochondrial DNA (mtDNA) marker. A total of 90 samples were collected from 9 localities within an area of the Subang Jaya Municipality. Genetic variability was determined through the amplification and sequencing of a fragment of the ND5 gene. Eight distinct mtDNA haplotypes were identified. The evolutionary relationship of the local haplotypes alongside 28 reference strains was used to construct a phylogram, the analysis of which revealed low genetic differentiation in terms of both nucleotide and haplotype diversity. Bayesian method was used to infer the phylogenetic tree, revealing a unique relationship between local isolates. The study corroborates the reliability of ND5 to identify distinct lineages for polymorphism-based studies and supplements the existing body of knowledge regarding its genetic diversity. This in turn could potentially aid existing vector control strategies to help mitigate the risk and spread of the dengue virus.
authors.forename: Nurul
authors.initials: N
authors.lastname: Adilah-Amrannudin
authors.forename: Mayamin
authors.initials: M
authors.lastname: Hamsidi
authors.forename: Nurul-Ain
authors.initials: NA
authors.lastname: Ismail
authors.forename: Rodziah
authors.initials: R
authors.lastname: Ismail
authors.forename: Nazri Che
authors.initials: NC
authors.lastname: Dom
authors.forename: Abu Hassan
authors.initials: AH
authors.lastname: Ahmad
authors.forename: Mohd Fahmi
authors.initials: MF
authors.lastname: Mastuki
authors.forename: Tengku Shahrul Anuar Tengku Ahmad
authors.initials: TS
authors.lastname: Basri
authors.forename: Adira
authors.initials: A
authors.lastname: Khalid
authors.forename: Mohammad
authors.initials: M
authors.lastname: Muslim
authors.forename: Nurul Amalina Ahmad
authors.initials: NA
authors.lastname: Daud
authors.forename: Siti Nazrina
authors.initials: SN
authors.lastname: Camalxaman
mesh_terms: D000330:Aedes / Q000235:genetics*; D000818:Animals; D001499:Bayes Theorem; D005075:Biological Evolution; D004272:DNA, Mitochondrial / Q000235:genetics*; D005819:Genetic Markers; D019476:Insect Proteins / Q000235:genetics*; D008296:Malaysia; D010802:Phylogeny; D011110:Polymorphism, Genetic
chemical_list: D004272:DNA, Mitochondrial; D005819:Genetic Markers; D019476:Insect Proteins
keywords: Aedes albopictus; genetic variation; haplotype; nicotinamide adenine dinucleotide dehydrogenase 5 subunit; phylogenetic tree