title: Chromatin remodeller Fun30Fft3induces nucleosome disassembly to facilitate RNA polymerase II elongation.
abstract: Previous studies have revealed that nucleosomes impede elongation of RNA polymerase II (RNAPII). Recent observations suggest a role for ATP-dependent chromatin remodellers in modulating this process, but direct in vivo evidence for this is unknown. Here using fission yeast, we identify Fun30Fft3as a chromatin remodeller, which localizes at transcribing regions to promote RNAPII transcription. Fun30Fft3associates with RNAPII and collaborates with the histone chaperone, FACT, which facilitates RNAPII elongation through chromatin, to induce nucleosome disassembly at transcribing regions during RNAPII transcription. Mutants, resulting in reduced nucleosome-barrier, such as deletion mutants of histones H3/H4 themselves and the genes encoding components of histone deacetylase Clr6 complex II suppress the defects in growth and RNAPII occupancy of cells lacking Fun30Fft3. These data suggest that RNAPII utilizes the chromatin remodeller, Fun30Fft3, to overcome the nucleosome barrier to transcription elongation.
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, South Korea.
authors.forename: Junwoo
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, South Korea.
authors.forename: Eun Shik
authors.initials: ES
authors.lastname: Choi
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, South Korea.
authors.forename: Hogyu David
authors.initials: HD
authors.lastname: Seo
authors.affiliation: Department of Microbiology, Dankook University, Cheonan, Chungnam 31116, South Korea.
authors.forename: Keunsoo
authors.initials: K
authors.lastname: Kang
authors.affiliation: Stowers Institute for Medical Research, Kansas City, Kansas City, Missouri 64110, USA.
authors.forename: Joshua M
authors.initials: JM
authors.lastname: Gilmore
authors.affiliation: Stowers Institute for Medical Research, Kansas City, Kansas City, Missouri 64110, USA.
authors.forename: Laurence
authors.initials: L
authors.lastname: Florens
authors.affiliation: Stowers Institute for Medical Research, Kansas City, Kansas City, Missouri 64110, USA.|Department of Anatomy and Cell Biology, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, Kansas 66160, USA.
authors.forename: Michael P
authors.identifier: 0000-0001-7568-2585
authors.initials: MP
authors.lastname: Washburn
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, South Korea.
authors.forename: Joonho
authors.initials: J
authors.lastname: Choe
authors.affiliation: Stowers Institute for Medical Research, Kansas City, Kansas City, Missouri 64110, USA.
authors.forename: Jerry L
authors.initials: JL
authors.lastname: Workman
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, South Korea.
authors.forename: Daeyoup
authors.initials: D
authors.lastname: Lee
mesh_terms: D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D042002:Chromatin Assembly and Disassembly; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D006657:Histones / Q000235:genetics / Q000378:metabolism; D008957:Models, Genetic; D009707:Nucleosomes / Q000235:genetics / Q000378:metabolism*; D012319:RNA Polymerase II / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D018797:Cell Cycle Proteins; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; C115303:Clr6 protein, S pombe; C559589:Fft3 protein, S pombe; D006657:Histones; D009707:Nucleosomes; D029702:Schizosaccharomyces pombe Proteins; D012319:RNA Polymerase II