title: ORAI1-dependent synaptic plasticity in rat hippocampal neurons.
abstract: It is well established that neurons are plastic and can change the strength of their connections with other neurons depending on their recent history. While many molecular entities involved in plastic processes were already described, the role of a store-operated calcium channel ORAI1 in neuronal plasticity is not known as yet. Using dominant negative form of ORAI1, we were able to show that ORAI1 is needed for formation of new dendritic spines following chemical induction of long term potentiation (cLTP), and that this is due to the release of Ca+2from ryanodine receptor-associated endoplasmic reticulum stores. We propose that when ORAI1 is deficient, there is less Ca+2in the stores, less releasable Ca+2and consequently less LTP and spine formation.
authors.affiliation: Department of Neurobiology, The Weizmann Institute, Rehovot, Israel.
authors.forename: Rami Yair
authors.initials: RY
authors.lastname: Tshuva
authors.affiliation: Department of Neurobiology, The Weizmann Institute, Rehovot, Israel.
authors.forename: Eduard
authors.initials: E
authors.lastname: Korkotian
authors.affiliation: Department of Neurobiology, The Weizmann Institute, Rehovot, Israel. Electronic address: menahem.segal@weizmann.ac.il.
authors.forename: Menahem
authors.initials: M
authors.lastname: Segal
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D049229:Dendritic Spines / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D009473:Neuronal Plasticity / Q000235:genetics*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D000071740:ORAI1 Protein / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D019837:Ryanodine Receptor Calcium Release Channel / Q000378:metabolism
chemical_list: D000071740:ORAI1 Protein; C532493:Orai1 protein, rat; D019837:Ryanodine Receptor Calcium Release Channel; D002118:Calcium
keywords: Dendritic spines; ER; LTP; NMDAR; ORAI1; Ryanodine receptor (RyR)