title: Response of the antioxidant enzymes of the erythrocyte and alterations in the serum biomarkers in rats following oral administration of nanoparticles.
abstract: In this study, Al2O3, CuO and TiO2nanoparticles (NPs) were administered to mature female rats (Rattus norvegicus var. albinos) via oral gavage (0, 0.5, 5, 50mg/kg b.w./day) for 14days to investigate their effects on 14 serum biomarkers and 4 antioxidant enzyme (catalase, superoxide dismutase, glutathione peroxidase, glutathione S-transferase) activities in the erythrocyte. Data showed that Al2O3did not cause any significant (P>0.05) change in the parameters, except few cases, while CuO and TiO2caused significant alterations in antioxidant system parameters of the erythrocytes. Activities of catalase and superoxide dismutase significantly decreased in CuO and TiO2administered rats. Oppositely, glutathione peroxidase activity increased in CuO and TiO2administered rats. There were no significant alterations in the activity of glutathione S-transferase in the erythrocytes. Levels of glucose, cholesterol, bilirubin, triglyceride, triiodothyronine (T3), estradiol, prolactin and immunoglobulin M (IgM) in the serum altered after some of NP administrations, whereas cortisol, protein, creatinine, blood urea nitrogen (BUN), thyroxine (T4) and immunoglobulin G (IgG) levels in the serum did not change significantly after any of NP administration. There were outstanding increases in the levels of bilirubin and prolactin and decreases in the levels of triglyceride and estradiol. The present study demonstrated that the antioxidant enzymes in the erythrocyte were generally affected from copper and titanium NPs, while aluminium and copper NPs caused more significant alterations in serum biomarkers.
authors.affiliation: University of Cukurova, Faculty of Sciences and Arts, Department of Biology, Adana, Turkey. Electronic address: egcanli@gmail.com.
authors.forename: Esin G
authors.initials: EG
authors.lastname: Canli
authors.affiliation: University of Cukurova, Vocational School of Imamoglu, Adana, Turkey.
authors.forename: Gülüzar
authors.initials: G
authors.lastname: Atli
authors.affiliation: University of Cukurova, Faculty of Sciences and Arts, Department of Biology, Adana, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Canli
mesh_terms: D000284:Administration, Oral; D000537:Aluminum Oxide / Q000633:toxicity*; D000818:Animals; D015415:Biomarkers / Q000097:blood*; D003300:Copper / Q000633:toxicity*; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism; D014025:Titanium / Q000633:toxicity*
chemical_list: D015415:Biomarkers; C009495:titanium dioxide; D003300:Copper; D014025:Titanium; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D000537:Aluminum Oxide; C030973:cupric oxide
keywords: Antioxidant; Erythrocyte; Metal; Nanoparticle; Rat; Serum