title: Therapeutic effects of human adipose tissue-derived stem cell (hADSC) transplantation on experimental autoimmune encephalomyelitis (EAE) mice.
abstract: This study is to investigate the therapeutic effects of human adipose tissue-derived stem cell (hADSC) transplantation on experimental autoimmune encephalomyelitis (EAE) in mice. EAE mouse model was established by MOG35-55 immunization. Body weight and neurological function were assessed. H&E and LFB staining was performed to evaluate histopathological changes. Flow cytometry was used to detect Th17 and Treg cells. ELISA and real-time PCR were performed to determine transcription factor and pro-inflammatory cytokine levels. Transplantation of hADSCs significantly alleviated the body weight loss and neurological function impairment of EAE mice. Inflammatory cell infiltration and demyelination were significantly increased, which were relieved by hADSC transplantation. Moreover, the Th17 cells and the ROR-γt mRNA level were significantly elevated, while the Treg cells and the Foxp3 mRNA level were significantly declined, resulting in significantly increased Th17/Treg ratio. This was reversed by the transplantation of hADSCs. Furthermore, serum levels of IL-17A, IL-6, IL-23, and TGF-β, were significantly increased, which could be influenced by the hADSC transplantation. Transplantation of hADSCs alleviates the neurological function impairment and histological changes, and reduces the inflammatory cell infiltration and demyelination in EAE mice, which might be associated with the regulation of Th17/Treg balance.
authors.affiliation: Department of Neurology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 32500, Zhejiang, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Neurology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 32500, Zhejiang, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Neurology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 32500, Zhejiang, China.
authors.forename: Zhibo
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Neurology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 32500, Zhejiang, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Neurology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 32500, Zhejiang, China.
authors.forename: Dehao
authors.initials: D
authors.lastname: Yang
authors.affiliation: Department of Neurology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 32500, Zhejiang, China.
authors.forename: Zhongqian
authors.initials: Z
authors.lastname: Su
authors.affiliation: Department of Neurology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 32500, Zhejiang, China.
authors.forename: Yiyun
authors.initials: Y
authors.lastname: Weng
authors.affiliation: Department of Neurology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 32500, Zhejiang, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Neurology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 32500, Zhejiang, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D000818:Animals; D015415:Biomarkers; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000175:diagnosis / Q000276:immunology / Q000628:therapy*; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D018836:Inflammation Mediators / Q000378:metabolism; D051379:Mice; D009103:Multiple Sclerosis / Q000175:diagnosis / Q000276:immunology / Q000628:therapy; D013116:Spinal Cord / Q000378:metabolism / Q000473:pathology; D013154:Spleen; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism; D058504:Th17 Cells / Q000276:immunology / Q000378:metabolism; D055815:Young Adult
chemical_list: D015415:Biomarkers; D016207:Cytokines; D018836:Inflammation Mediators