title: Cytotoxic Fusicoccane-Type Diterpenoids from Streptomyces violascens Isolated from Ailuropoda melanoleuca Feces.
abstract: Six new fusicoccane-type diterpenoids (2-7) were isolated from the fermentation broth of Streptomyces violascens, which was isolated from Ailuropoda melanoleuca (giant panda) feces. The structures of these new compounds were elucidated by a detailed spectroscopic data and X-ray crystallographic analysis. Compounds 5-7 demonstrated cytotoxicity against five human cancer cell lines, with IC50values ranging from 3.5 ± 0.7 to 14.1 ± 0.8 μM. Cell adhesion, migration, and invasion assays showed that 6 inhibited the migration and invasion of human hepatocellular carcinoma SMMC7721 cells in a dose-dependent manner. Through further investigation, it was revealed that 6 inhibited the enzymatic activity of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9), in addition to down-regulating the expressions of MMP-2 and MMP-9 at both the protein and mRNA levels to influence the migration and invasion of cancer cells.
authors.affiliation: Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University , Shenyang 110819, People's Republic of China.|Laboratory of Metabolic Disease Research and Drug Development, China Medical University , Shenyang 110001, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zheng
authors.affiliation: Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University , Shenyang 110819, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Han
authors.affiliation: Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University , Shenyang 110819, People's Republic of China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Qu
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University , Kunming 650091, People's Republic of China.
authors.forename: Xiu
authors.initials: X
authors.lastname: Chen
authors.affiliation: Shanghai Institute of Pharmaceutical Industry , Shanghai 201203, People's Republic of China.
authors.forename: Jialiang
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University , Shenyang 110819, People's Republic of China.
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Bi
authors.affiliation: Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University , Shenyang 110819, People's Republic of China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Liu
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University , Kunming 650091, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University , Kunming 650091, People's Republic of China.
authors.forename: Chenglin
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University , Shenyang 110819, People's Republic of China.
authors.forename: Xueshi
authors.identifier: 0000-0002-1561-8108
authors.initials: X
authors.lastname: Huang
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D008113:Liver Neoplasms; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D013302:Streptomyces / Q000737:chemistry*; D001503:Ursidae
chemical_list: D004224:Diterpenes; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9