title: Pre-breeding of lentil (Lens culinaris Medik.) for herbicide resistance through seed mutagenesis.
abstract: Lentil is a poor competitor of weeds and its sensitivity to herbicides is a major hurdle for large scale production. The present study was conducted to select herbicide resistant lentil genotypes through seed mutagenesis. Seeds of three advanced lentil genotypes (LPP 11001, LPP 11100 and LPP 11116) were treated with two different concentrations of ethyl methanesulfonate (EMS; 0.1 and 0.2%), hydrazine hydrate (HH; 0.02 and 0.03%) and sodium azide (SA; 0.01 and 0.02%) to develop M1 seed. The M2 was screened against two herbicides including Ally Max 28.6% SG (X = 34.58 g/ha and 1.5X = 51.87 g/ha) and Atlantis 3.6% WG (X = 395.2 g/ha and 1.5X = 592.8 g/ha) using the following three screening methods: post plant emergence (PPE), pre-plant incorporation (PPI) and seed priming (SP). Data were recorded on survival index and survival percentage from each experimental unit of every population. Plants in all populations were categorized following their reaction to herbicides. The newly developed populations showed greater variation for herbicide resistance when compared to their progenitors. Phenotypic traits were significantly reduced in all the screening environments. Overall, 671 herbicide resistant mutants were selected from all testing environments. The seeds from selected plants were re-mutagenized at 150 Gy of gamma radiation and evaluated against higher dose of herbicides. This allowed selection of 134 herbicide resistant mutants. The selected mutants are useful germplasm for herbicide resistance breeding of lentil.
authors.affiliation: Nuclear Institute for Agriculture and Biology, Faisalabad, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Rizwan
authors.affiliation: Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Faisalabad, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Aslam
authors.affiliation: Nuclear Institute for Agriculture and Biology, Faisalabad, Pakistan.
authors.forename: Muhammad Jawad
authors.initials: MJ
authors.lastname: Asghar
authors.affiliation: Nuclear Institute for Agriculture and Biology, Faisalabad, Pakistan.
authors.forename: Ghulam
authors.initials: G
authors.lastname: Abbas
authors.affiliation: Nuclear Institute for Agriculture and Biology, Faisalabad, Pakistan.
authors.forename: Tariq Mahmud
authors.initials: TM
authors.lastname: Shah
authors.affiliation: School of Agricultural, Earth and Environmental Sciences, African Centre for Crop Improvement, University of KwaZulu-Natal, Pietermaritzburg, South Africa.
authors.forename: Hussein
authors.initials: H
authors.lastname: Shimelis
mesh_terms: D002273:Carcinogens / Q000633:toxicity; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D005720:Gamma Rays; D017343:Genes, Plant / Q000235:genetics; D005828:Genetics, Population / Q000379:methods; D005838:Genotype; D053209:Herbicide Resistance / Q000235:genetics*; D006540:Herbicides / Q000494:pharmacology; D006834:Hydrazines / Q000633:toxicity; D027806:Lens Plant / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D016296:Mutagenesis / Q000187:drug effects / Q000528:radiation effects; D009153:Mutagens / Q000633:toxicity; D009154:Mutation; D010641:Phenotype; D000069600:Plant Breeding / Q000379:methods; D057685:Plant Weeds / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D012639:Seeds / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D019810:Sodium Azide / Q000633:toxicity
chemical_list: D002273:Carcinogens; D006540:Herbicides; D006834:Hydrazines; D009153:Mutagens; C029424:hydrazine; D019810:Sodium Azide; D005020:Ethyl Methanesulfonate