title: The inhibiting effect of the transcription factor p53 on dengue virus infection by activating the type I interferon.
abstract: To investigate the role of the transcription factor p53 in the course of the dengue virus (DV) infection. The human hepatocellular carcinoma cell strain HepG2 with a low expression level of p53 was built by using the retroviral-mediated RNA interference technology, and was detected by Western blot. The wild group and the interference group were respectively infected by the type 2 DV. The viral titration was detected by the Vero plaque assay, the viral multiplication was detected by the immunofluorescence, the cell apoptosis after virus infection was detected by FCM and the level of IFN-β was analyzed by ELISA. Compared to the wild group, the expression level of p53 in the interference group decreased significantly, which indicated that the HepG2 cell strain with the low expression level of p53 was successfully built. 24h after DV infection, the virus titration in the interference group was 100 times higher than that in the wild group. The result of the immunofluorescence showed that, the amount of green fluorescent cells in the interference group was significant higher than that in the wild group. It was indicated that the DV infection was inhibited by p53. However, 24h after DV infection, there was no significant difference in the amount of apoptotic cells in both groups. And the amount of IFN-β in the wild group increased 6 times. The DV infection was inhibited by the transcription factor p53 by activating type I interferon pathway other than promoting the cell apoptosis.
authors.affiliation: Department of Pathogen Biology and Immunology, Chongqing Three Gorges Medical College, Chongqing 404120, China.
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Hu
authors.affiliation: Department of Pathogen Biology and Immunology, Chongqing Three Gorges Medical College, Chongqing 404120, China.
authors.forename: Xiao-Shan
authors.initials: XS
authors.lastname: Li
authors.affiliation: Department of Pathogen Biology and Immunology, Chongqing Three Gorges Medical College, Chongqing 404120, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Xiong
authors.affiliation: Department of Pathogen Biology and Immunology, Chongqing Three Gorges Medical College, Chongqing 404120, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Department of Pathogen Biology and Immunology, Chongqing Three Gorges Medical College, Chongqing 404120, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Pathogen Biology and Immunology, Chongqing Three Gorges Medical College, Chongqing 404120, China.
authors.forename: Zhong-Quan
authors.initials: ZQ
authors.lastname: Shi
authors.affiliation: Department of Pathogen Biology and Immunology, Chongqing Three Gorges Medical College, Chongqing 404120, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Tang
authors.affiliation: Department of Microbiology, The Third Military Medical University, Chongqing 400038, China.
authors.forename: Xian-Cai
authors.initials: XC
authors.lastname: Rao
authors.affiliation: Department of Microbiology, The Third Military Medical University, Chongqing 400038, China.
authors.forename: Fu-Quan
authors.initials: FQ
authors.lastname: Hu
authors.affiliation: Department of Pathogen Biology and Immunology, Chongqing Three Gorges Medical College, Chongqing 404120, China.
authors.forename: Guo-Li
authors.initials: GL
authors.lastname: Li
mesh_terms: D017209:Apoptosis; D003715:Dengue / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D003716:Dengue Virus / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D056945:Hep G2 Cells; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis*; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D019562:Viral Load
chemical_list: D007370:Interferon Type I; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53
keywords: apoptosis; cell apoptosis; dengue virus; interferon; p53