title: Screening and Expression of a Silicon Transporter Gene(Lsi1)in Wild-Type Indica Rice Cultivars.
abstract: Silicon (Si) is one of the most prevalent elements in the soil. It is beneficial for plant growth and development, and it contributes to plant defense against different stresses. TheLsi1gene encodes a Si transporter that was identified in a mutant Japonica rice variety. This gene was not identified in fourteen Malaysian rice varieties during screening. Then, a mutant version ofLsi1was substituted for the native version in the three most common Malaysian rice varieties, MR219, MR220, and MR276, to evaluate the function of the transgene. Real-time PCR was used to explore the differential expression ofLsi1in the three transgenic rice varieties. Silicon concentrations in the roots and leaves of transgenic plants were significantly higher than in wild-type plants. Transgenic varieties showed significant increases in the activities of the enzymes SOD, POD, APX, and CAT; photosynthesis; and chlorophyll content; however, the highest chlorophyll A and B levels were observed in transgenic MR276. Transgenic varieties have shown a stronger root and leaf structure, as well as hairier roots, compared to the wild-type plants. This suggests thatLsi1plays a key role in rice, increasing the absorption and accumulation of Si, then alters antioxidant activities, and improves morphological properties.
authors.affiliation: Laboratory of Climate-Smart Food Crop Production, Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Mahbod
authors.identifier: 0000-0003-2165-484X
authors.initials: M
authors.lastname: Sahebi
authors.affiliation: Laboratory of Climate-Smart Food Crop Production, Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Laboratory of Plantation Science and Technology, Institute of Plantation Studies, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Mohamed M
authors.identifier: 0000-0003-1898-6819
authors.initials: MM
authors.lastname: Hanafi
authors.affiliation: Laboratory of Climate-Smart Food Crop Production, Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: M Y
authors.identifier: 0000-0003-4763-6367
authors.initials: MY
authors.lastname: Rafii
authors.affiliation: Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Parisa
authors.identifier: 0000-0001-5464-6761
authors.initials: P
authors.lastname: Azizi
authors.affiliation: Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Rambod
authors.initials: R
authors.lastname: Abiri
authors.affiliation: Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Nahid
authors.initials: N
authors.lastname: Kalhori
authors.affiliation: IAU of Tehran Science and Research Branch, Tehran, Iran.
authors.forename: Narges
authors.initials: N
authors.lastname: Atabaki
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D049452:Green Fluorescent Proteins / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D012275:Oryza / Q000235:genetics* / Q000648:ultrastructure; D010788:Photosynthesis; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism / Q000648:ultrastructure; D030821:Plants, Genetically Modified; D012038:Regeneration; D012639:Seeds / Q000378:metabolism; D012825:Silicon / Q000378:metabolism*; D019076:Transgenes
chemical_list: D000975:Antioxidants; D026901:Membrane Transport Proteins; D010940:Plant Proteins; D002734:Chlorophyll; D049452:Green Fluorescent Proteins; D012825:Silicon