title: Isolation and identification of N-butyl-tetrahydro-5-oxofuran-2-carboxamide produced by Bacillus sp. L60 and its antifungal activity.
abstract: Rhizoctonia solani is the cause of substantial economic loss in many crops. The aim of this study is to investigate biocontrol potential of Bacillus sp. L60 against R. solani and to purify an antifungal compound. In this study, Bacillus sp. L60 demonstrated significant antagonism toward R. solani with the dual culture assay. The antifungal compound was extracted from Bacillus sp. L60 culture supernatant with n-butanol, and identified as N-butyl-tetrahydro-5-oxofuran-2-carboxamide (BT-5O-2C) having molecular weights of 185.1052 Da with the formula C9H15NO3using NMR and HR-ESI-MS analysis. The minimum inhibitory concentration (MIC) value of the antifungal compound was 256 µg ml-1against R. solani. Therefore, our results clearly demonstrated BT-5O-2C as well as Bacillus sp. L60 as potential biological control agents for the management of R. solani.
authors.affiliation: Division of Food Technology, Biotechnology and Agrochemistry, Institute of Environmentally-Friendly Agriculture, Chonnam National University, Gwangju, Korea.
authors.forename: Yong-Seong
authors.initials: YS
authors.lastname: Lee
authors.affiliation: Department of Food Engineering, Mokpo National University, Mokpo, Korea.
authors.forename: Jeong-Yong
authors.initials: JY
authors.lastname: Cho
authors.affiliation: Department of Food Science and Technology, and Functional Food Research Center, Chonnam National University, Gwangju, Korea.
authors.forename: Jae-Hak
authors.initials: JH
authors.lastname: Moon
authors.affiliation: Division of Food Technology, Biotechnology and Agrochemistry, Institute of Environmentally-Friendly Agriculture, Chonnam National University, Gwangju, Korea.
authors.forename: Kil-Yong
authors.initials: KY
authors.lastname: Kim
mesh_terms: D020001:1-Butanol / Q000494:pharmacology; D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D000898:Antibiosis; D000935:Antifungal Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001407:Bacillus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D056910:Republic of Korea; D012232:Rhizoctonia / Q000187:drug effects / Q000502:physiology*; D012988:Soil Microbiology
chemical_list: D000935:Antifungal Agents; D003470:Culture Media; C000616252:N-butyl-tetrahydro-5-oxofuran-2-carboxamide; D020001:1-Butanol; D015107:4-Butyrolactone
keywords: Bacillus sp. L60; N-butyl-tetrahydro-5-oxofuran-2-carboxamide (BT-5O-2C); Rhizoctonia solani; antifungal compound; bioocontrol