title: Effect of a one-step self-etch adhesive on endogenous dentin matrix metalloproteinases.
abstract: Degradation of the hybrid layer created in dentin by dentin adhesives is caused by enzyme activities present within the dentin matrix that destroy unprotected collagen fibrils. The aim of the present study was to evaluate the effect of a one-step self-etch adhesive system on dentinal matrix metalloproteinases 2 and 4 (MMP-2 and MMP-9, respectively) using in situ zymography and an enzymatic activity assay. The null hypothesis tested was that there are no differences in the activities of dentinal MMPs before and after treatment with a one-step adhesive system. The MMP-2 and MMP-9 activities in dentin treated with the one-step adhesive, Adper Easy Bond, were quantified using an enzymatic activity assay system. The MMP activities within the hybrid layer created by the one-step adhesive tested were also evaluated using in situ zymography. The enzymatic assay revealed an increase in MMP-2 and MMP-9 activities after treatment with adhesive. In situ zymography indicated that gelatinolytic activity is present within the hybrid layer created with the one-step self-etch adhesive. The host-derived gelatinases were localized within the hybrid layer and remained active after the bonding procedure. It is concluded that the one-step self-etch adhesive investigated activates endogenous MMP-2 and MMP-9 with the dentin matrix, which may cause collagen degradation over time.
authors.affiliation: Division of Dentistry, Manaus Air Force Hospital - Brazilian Air Force, Contorno Avenue, Manaus, Amazonas, Brazil.
authors.forename: Fabianni M
authors.initials: FM
authors.lastname: Apolonio
authors.affiliation: Department of Biomedical and Neuromotor Sciences, DIBINEM, University of Bologna - Alma Mater Studiorum, Bologna, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Mazzoni
authors.affiliation: Department of Biomedical and Neuromotor Sciences, DIBINEM, University of Bologna - Alma Mater Studiorum, Bologna, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Angeloni
authors.affiliation: Department of Oral Biology, Bauru School of Dentistry, University of São Paulo (USP), Bauru, Brazil.
authors.forename: Polliana M C
authors.initials: PM
authors.lastname: Scaffa
authors.affiliation: Institute of Molecular Genetics, CNR-National Research Council of Italy, Bologna, Italy.|SC Laboratory of Musculoskeletal Cell Biology, Rizzoli Orthopedic Institute, Bologna, Italy.
authors.forename: Spartaco
authors.initials: S
authors.lastname: Santi
authors.affiliation: Department of Restorative Dentistry, School of Dentistry, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Vicente de Paulo A
authors.initials: VP
authors.lastname: Saboia
authors.affiliation: Department of Oral Biology, College of Dental Medicine, Georgia Regents University, Augusta, GA, USA.
authors.forename: Franklin R
authors.initials: FR
authors.lastname: Tay
authors.affiliation: Department of Oral Biology, College of Dental Medicine, Georgia Regents University, Augusta, GA, USA.
authors.forename: David H
authors.initials: DH
authors.lastname: Pashley
authors.affiliation: Department of Biomedical and Neuromotor Sciences, DIBINEM, University of Bologna - Alma Mater Studiorum, Bologna, Italy.
authors.forename: Lorenzo
authors.identifier: 0000-0001-7621-226X
authors.initials: L
authors.lastname: Breschi
mesh_terms: D003188:Composite Resins / Q000737:chemistry*; D003804:Dentin / Q000187:drug effects* / Q000201:enzymology*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D008964:Molar, Third
chemical_list: D003188:Composite Resins; D017220:Dentin-Bonding Agents; C584661:easy bond; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9
keywords: biochemical assays; collagen; dentin bonding systems; hybrid layer; zymography