title: DNA-wrapped multi-walled carbon nanotube modified electrochemical biosensor for the detection of Escherichia coli from real samples.
abstract: This paper introduces DNA-wrapped multi-walled carbon nanotube (MWCNT)-modified genosensor for the detection of Escherichia coli (E. coli) from polymerase chain reaction (PCR)-amplified real samples while Staphylococcus aureus (S. aureus) was used to investigate the selectivity of the biosensor. The capture probe specifically recognizing E. coli DNA and it was firstly interacted with MWCNTs for wrapping of single-stranded DNA (ssDNA) onto the nanomaterial. DNA-wrapped MWCNTs were then immobilised on the surface of disposable pencil graphite electrode (PGE) for the detection of DNA hybridization. Electrochemical behaviors of the modified PGEs were investigated using Raman spectroscopy and differential pulse voltammetry (DPV). The sequence selective DNA hybridization was determined and evaluated by changes in the intrinsic guanine oxidation signal at about 1.0V by DPV. Numerous factors affecting the hybridization were optimized such as target concentration, hybridization time, etc. The designed DNA sensor can well detect E. coli DNA in 20min detection time with 0.5pmole of detection limit in 30µL of sample volume.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey. Electronic address: dilsat.ariksoysal@ege.edu.tr.
authors.forename: Dilsat
authors.initials: D
authors.lastname: Ozkan-Ariksoysal
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey.
authors.forename: Yasin Ugur
authors.initials: YU
authors.lastname: Kayran
authors.affiliation: Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey.
authors.forename: Fethiye Ferda
authors.initials: FF
authors.lastname: Yilmaz
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, University of Bucharest, 90-92 Panduri Avenue, District 5, 050663 Bucharest, Romania.
authors.forename: Anton Alexandru
authors.initials: AA
authors.lastname: Ciucu
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, University of Bucharest, 90-92 Panduri Avenue, District 5, 050663 Bucharest, Romania.
authors.forename: Iulia Gabriela
authors.initials: IG
authors.lastname: David
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, University of Bucharest, 90-92 Panduri Avenue, District 5, 050663 Bucharest, Romania.
authors.forename: Vasile
authors.initials: V
authors.lastname: David
authors.affiliation: Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey.
authors.forename: Mine
authors.initials: M
authors.lastname: Hosgor-Limoncu
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Ozsoz
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D048271:Chitosan / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics; D004563:Electrochemistry; D004566:Electrodes; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D013211:Staphylococcus aureus / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D037742:Nanotubes, Carbon; D048271:Chitosan
keywords: DNA Hybridization; DNA-wrapped multi-walled carbon nanotubes; Electrochemical DNA biosensor; Escherichia coli detection; Pencil graphite electrode (PGE); Polymerase chain reaction (PCR) amplified real samples