title: Mitochondria-Targeted Ratiometric Fluorescent Nanosensor for Simultaneous Biosensing and Imaging of O2•-and pH in Live Cells.
abstract: Intracellular pH undertakes critical functions in the formation of a proton gradient and electrochemical potential that drives the adenosine triphosphate synthesis. It is also involved in various metabolic processes occurring in mitochondria, such as the generation of reactive oxygen species, calcium regulation, as well as the triggering of cell proliferation and apoptosis. Meanwhile, the aberrant accumulation of O2•-within mitochondria is frequently intertwined with mitochondrial dysfunction and disease development. To disentangle the complicated inter-relationship between pH and O2•-in the signal transduction and homeostasis in mitochondria, herein we developed a mitochondria-targeted single fluorescent probe for simultaneous sensing and imaging of pH and O2•-in mitochondria. CdSe/ZnS quantum dots encapsulated in silica shell was designed as an inner reference element for providing a built-in correction, as well as employed as a carrier to assemble the responsive elements for O2•-and pH, together with mitochondria-targeted molecule. The developed nanosensor demonstrated high accuracy and selectivity for pH and O2•-sensing, against other ROS, metal ions, and amino acids. The remarkable analytical performance of the present nanosensor, as well as good biocompatibility, established an accurate and selective approach for real-time imaging and biosensing of O2•-and pH in mitochondria of live cells.
authors.affiliation: Shanghai State Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, East China Normal University , Dongchuan Road 500, Shanghai 200241, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Huang
authors.affiliation: Shanghai State Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, East China Normal University , Dongchuan Road 500, Shanghai 200241, China.
authors.forename: Fangyuan
authors.initials: F
authors.lastname: Dong
authors.affiliation: Shanghai State Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, East China Normal University , Dongchuan Road 500, Shanghai 200241, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Tian
mesh_terms: D000818:Animals; D017209:Apoptosis; D015374:Biosensing Techniques / Q000379:methods; D019187:Cadmium Compounds / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008928:Mitochondria / Q000737:chemistry*; D061848:Optical Imaging / Q000379:methods*; D045663:Quantum Dots / Q000737:chemistry*; D000067996:RAW 264.7 Cells; D017382:Reactive Oxygen Species / Q000032:analysis*; D018036:Selenium Compounds / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods; D013440:Sulfides / Q000737:chemistry*; D017967:Zinc Compounds / Q000737:chemistry*
chemical_list: D019187:Cadmium Compounds; D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; D012822:Silicon Dioxide; C058667:cadmium selenide; C031238:zinc sulfide