title: Chemical constituents of Pholidota cantonensis.
abstract: Two 9,10-dihydrophenanthrenes trivially named phocantol and phocantone, two diterpenoid glycosidesnamed phocantoside A and phocantoside B were isolated from the ethanol extract of the air-dried whole plant of Pholidota cantonensis Rolfe, together with seventeen known compounds. The structures of the four compounds were identified as 1-hydroxy-2,7-dimethoxy-9,10-dihydrophenanthro-[4,5-bcd]furan, 5-hydroxy-2,7-dimethoxy-9,10-dihydro-1,4-phenanthrenedione, (8R,13E)-ent-labd-13-ene-3α,8,15-triol 15-O-β-D-gluco-pyranoside and (5S,8R,9S,10R)-cis-cleroda-3,13(E)-diene-15,18-diol 15-O-β-D-glucopyranosyl-18-O-β-D-glucopyranoside by chemical and spectroscopic methods, including 1D and 2D NMR. Twenty compounds were evaluated for their cytotoxic activities against mouse leukemia p388D1 cancer cells, and compound phocantone, phocantoside A, tanshinone IIA and syringate exhibited cytotoxic activity against the mouse leukemia p388D1 cancer cells with IC50values ranging from 13.37 to 27.5 μM.
authors.affiliation: Hunan Province Engineering Research Center of Bioactive Substance Discovery of Chinese Medicine, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China; TCM and Ethnomedicine Innovation & Development Laboratory, Sino-Luxemburg TCM Research Center, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: National Center for Natural Products Research, University of Mississippi, Oxford, MS 38677, USA.
authors.forename: Zulfiqar
authors.initials: Z
authors.lastname: Ali
authors.affiliation: Natural Health and Food Products Research Group, British Columbia Institute of Technology, Burnaby V5G3H2, BC, Canada.
authors.forename: Michael
authors.initials: M
authors.lastname: Chan
authors.affiliation: Hunan Province Engineering Research Center of Bioactive Substance Discovery of Chinese Medicine, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: National Center for Natural Products Research, University of Mississippi, Oxford, MS 38677, USA.
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.affiliation: National Center for Natural Products Research, University of Mississippi, Oxford, MS 38677, USA.
authors.forename: Naohito
authors.initials: N
authors.lastname: Abe
authors.affiliation: Hunan Province Engineering Research Center of Bioactive Substance Discovery of Chinese Medicine, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China.
authors.forename: Can-Rong
authors.initials: CR
authors.lastname: Wu
authors.affiliation: National Center for Natural Products Research, University of Mississippi, Oxford, MS 38677, USA; Department of Pharmacognosy, University of Mississippi, Oxford, MS 38677, USA.
authors.forename: Ikhlas A
authors.initials: IA
authors.lastname: Khan
authors.affiliation: Hunan Province Engineering Research Center of Bioactive Substance Discovery of Chinese Medicine, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China; TCM and Ethnomedicine Innovation & Development Laboratory, Sino-Luxemburg TCM Research Center, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. Electronic address: wangwei402@hotmail.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Hunan Province Engineering Research Center of Bioactive Substance Discovery of Chinese Medicine, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. Electronic address: lishunxiang@hotmail.com.
authors.forename: Shun-Xiang
authors.initials: SX
authors.lastname: Li
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification; D045744:Cell Line, Tumor; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification; D051379:Mice; D015394:Molecular Structure; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors; D029595:Orchidaceae / Q000737:chemistry*; D010616:Phenanthrenes / Q000737:chemistry / Q000302:isolation & purification; D064209:Phytochemicals / Q000737:chemistry* / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry*; D000067996:RAW 264.7 Cells
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D006027:Glycosides; D010616:Phenanthrenes; D064209:Phytochemicals; D010936:Plant Extracts; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse
keywords: 9,10-Dihydrophenanthrene; Cytotoxicity; Diterpenoid glycosides; Orchidaceae; Pholidota cantonensis