title: Phenotypic ConsequencesIn vivoandIn vitroof Rearranging the P Gene of RABV HEP-Flury.
abstract: Phosphoprotein (P) of the Rabies virus (RABV) is critically required for viral replication and pathogenicity. Here we manipulated infectious cDNA clones of the RABV HEP-Flury to translocate the P gene from its wild-type position 2 to 1, 3, or 4 in gene order, using an approach which left the viral nucleotide sequence unaltered. The recovered viruses were evaluated for the levels of gene expression, growth kinetics in cell culture, lethality in suckling mice and protection of mice. The results showed that viral replication was affected by the absolute value of N protein which was regulated by P protein. Viral lethality in suckling mice was consistent with the ratio of P mRNA in one complete transcription. The protection of mice induced by viruses was related to the antibody titer 5 weeks post-infection which might be regulated by G protein. However, the ability to induce cell apoptosis and viral spread were not only related to the viral replication but also to the ratio of related gene which affected by the gene position. These findings might not only improve the understanding of phenotype of RABV and P gene rearrangement, but also help rabies vaccine candidate construction.
authors.affiliation: College of Veterinary Medicine, South China Agricultural UniversityGuangzhou, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong ProvinceGuangzhou, China.
authors.forename: Mingzhu
authors.initials: M
authors.lastname: Mei
authors.affiliation: College of Veterinary Medicine, South China Agricultural UniversityGuangzhou, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong ProvinceGuangzhou, China.
authors.forename: Teng
authors.initials: T
authors.lastname: Long
authors.affiliation: College of Veterinary Medicine, South China Agricultural UniversityGuangzhou, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong ProvinceGuangzhou, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, South China Agricultural UniversityGuangzhou, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong ProvinceGuangzhou, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: College of Veterinary Medicine, South China Agricultural UniversityGuangzhou, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong ProvinceGuangzhou, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Tian
authors.affiliation: College of Veterinary Medicine, South China Agricultural UniversityGuangzhou, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong ProvinceGuangzhou, China.
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Peng
authors.affiliation: College of Veterinary Medicine, South China Agricultural UniversityGuangzhou, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong ProvinceGuangzhou, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Luo
authors.affiliation: College of Veterinary Medicine, South China Agricultural UniversityGuangzhou, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong ProvinceGuangzhou, China.
authors.forename: Yifei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, South China Agricultural UniversityGuangzhou, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong ProvinceGuangzhou, China.
authors.forename: Yingyi
authors.initials: Y
authors.lastname: Lin
authors.affiliation: College of Veterinary Medicine, South China Agricultural UniversityGuangzhou, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong ProvinceGuangzhou, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Guo
keywords: HEP-Flury; Rabies virus; gene rearrangement; pathogenicity; phosphoprotein