title: CD4+CD25+GARP+regulatory T cells display a compromised suppressive function in patients with dilated cardiomyopathy.
abstract: Dilated cardiomyopathy (DCM) is a lethal inflammatory heart disease and closely connected with dysfunction of the immune system. Glycoprotein A repetitions predominant (GARP) expressed on activated CD4+T cells with suppressive activity has been established. This study aimed to investigate the frequency and function of circulating CD4+CD25+GARP+regulatory T (Treg) cells in DCM. Forty-five DCM patients and 46 controls were enrolled in this study. There was a significant increase in peripheral T helper type 1 (Th1) and Th17 number and their related cytokines [interferon-γ (IFN-γ), interleukin (IL-17)], and an obvious decrease in Treg number, transforming growth factor-β1(TGF-β1) levels and the expression of forkhead box P3 (FOXP3) and GARP in patients with DCM compared with controls. In addition, the suppressive function of CD4+CD25+GARP+Treg cells was impaired in DCM patients upon T-cell receptor stimulation detected using CFSE dye. Lower level of TGF-β1and higher levels of IFN-γ and IL-17 detected using ELISA were found in supernatants of the cultured CD4+CD25+GARP+Treg cells in DCM patients compared with controls. Together, our results indicate that CD4+CD25+GARP+Treg cells are defective in DCM patients and GARP seems to be a better molecular definition of the regulatory phenotype. Therefore, it might be an attractive stategy to pay more attention to GARP in DCM patients.
authors.affiliation: Laboratory of Cardiovascular Immunology, Institute of Cardiology, Union Hospital, TongJi Medical College, Huahzong University of Science and Technology, Wuhan, China.
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Laboratory of Cardiovascular Immunology, Institute of Cardiology, Union Hospital, TongJi Medical College, Huahzong University of Science and Technology, Wuhan, China.
authors.forename: Kunwu
authors.initials: K
authors.lastname: Yu
authors.affiliation: The First Peoples Hospital of Tianmen City, Tianmen, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wei
authors.affiliation: Laboratory of Cardiovascular Immunology, Institute of Cardiology, Union Hospital, TongJi Medical College, Huahzong University of Science and Technology, Wuhan, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Su
authors.affiliation: Laboratory of Cardiovascular Immunology, Institute of Cardiology, Union Hospital, TongJi Medical College, Huahzong University of Science and Technology, Wuhan, China.
authors.forename: Ruirui
authors.initials: R
authors.lastname: Zhu
authors.affiliation: Laboratory of Cardiovascular Immunology, Institute of Cardiology, Union Hospital, TongJi Medical College, Huahzong University of Science and Technology, Wuhan, China.
authors.forename: Huairui
authors.initials: H
authors.lastname: Shi
authors.affiliation: Laboratory of Cardiovascular Immunology, Institute of Cardiology, Union Hospital, TongJi Medical College, Huahzong University of Science and Technology, Wuhan, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Sun
authors.affiliation: Laboratory of Cardiovascular Immunology, Institute of Cardiology, Union Hospital, TongJi Medical College, Huahzong University of Science and Technology, Wuhan, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Laboratory of Cardiovascular Immunology, Institute of Cardiology, Union Hospital, TongJi Medical College, Huahzong University of Science and Technology, Wuhan, China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Xu
authors.affiliation: Laboratory of Cardiovascular Immunology, Institute of Cardiology, Union Hospital, TongJi Medical College, Huahzong University of Science and Technology, Wuhan, China.
authors.forename: Junhui
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Laboratory of Cardiovascular Immunology, Institute of Cardiology, Union Hospital, TongJi Medical College, Huahzong University of Science and Technology, Wuhan, China.
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: Laboratory of Cardiovascular Immunology, Institute of Cardiology, Union Hospital, TongJi Medical College, Huahzong University of Science and Technology, Wuhan, China.
authors.forename: Qiutang
authors.identifier: 0000-0002-2192-3635
authors.initials: Q
authors.lastname: Zeng
mesh_terms: D056704:Adaptive Immunity; D000328:Adult; D018791:CD4 Lymphocyte Count; D002311:Cardiomyopathy, Dilated / Q000175:diagnosis / Q000276:immunology* / Q000378:metabolism; D016022:Case-Control Studies; D002450:Cell Communication; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D005260:Female; D051858:Forkhead Transcription Factors / Q000276:immunology / Q000378:metabolism; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology* / Q000378:metabolism; D008213:Lymphocyte Activation; D008297:Male; D008565:Membrane Proteins / Q000276:immunology* / Q000378:metabolism; D008875:Middle Aged; D010641:Phenotype; D015398:Signal Transduction; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism; D058504:Th17 Cells / Q000276:immunology / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000276:immunology / Q000378:metabolism
chemical_list: D016207:Cytokines; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; C508017:IL2RA protein, human; D053645:Interleukin-2 Receptor alpha Subunit; C087215:LRRC32 protein, human; D008565:Membrane Proteins; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1
keywords: dilated cardiomyopathy; glycoprotein A repetitions predominant; immune system; regulatory T cells