title: A rapid, efficient, and economic device and method for the isolation and purification of mouse islet cells.
abstract: Rapid, efficient, and economic method for the isolation and purification of islets has been pursued by numerous islet-related researchers. In this study, we compared the advantages and disadvantages of our developed patented method with those of commonly used conventional methods (Ficoll-400, 1077, and handpicking methods). Cell viability was assayed using Trypan blue, cell purity and yield were assayed using diphenylthiocarbazone, and islet function was assayed using acridine orange/ethidium bromide staining and enzyme-linked immunosorbent assay-glucose stimulation testing 4 days after cultivation. The results showed that our islet isolation and purification method required 12 ± 3 min, which was significantly shorter than the time required in Ficoll-400, 1077, and HPU groups (34 ± 3, 41 ± 4, and 30 ± 4 min, respectively; P < 0.05). There was no significant difference in islet viability among the four groups. The islet purity, function, yield, and cost of our method were superior to those of the Ficoll-400 and 1077 methods, but inferior to the handpicking method. However, the handpicking method may cause wrist injury and visual impairment in researchers during large-scale islet isolation (>1000 islets). In summary, the MCT method is a rapid, efficient, and economic method for isolating and purifying murine islet cell clumps. This method overcomes some of the shortcomings of conventional methods, showing a relatively higher quality and yield of islets within a shorter duration at a lower cost. Therefore, the current method provides researchers with an alternative option for islet isolation and should be widely generalized.
authors.affiliation: Department of Hepatobiliary Surgery and Organ Transplantation, the First Hospital of China Medical University, Shenyang, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Zongyi
authors.affiliation: Department of Hepatobiliary Surgery and Organ Transplantation, the First Hospital of China Medical University, Shenyang, China.
authors.forename: Zou
authors.initials: Z
authors.lastname: Funian
authors.affiliation: Department of Hepatobiliary Surgery and Organ Transplantation, the First Hospital of China Medical University, Shenyang, China.
authors.forename: Li
authors.initials: L
authors.lastname: Hao
authors.affiliation: Department of Hepatobiliary Surgery and Organ Transplantation, the First Hospital of China Medical University, Shenyang, China.|National Key Lab. of General Surgery, the First Hospital of China Medical University, Shenyang, China.|Multiple Organ Transplantation Institute of the First Hospital of China Medical University, Shenyang, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Ying
authors.affiliation: Department of Hepatobiliary Surgery and Organ Transplantation, the First Hospital of China Medical University, Shenyang, China.|National Key Lab. of General Surgery, the First Hospital of China Medical University, Shenyang, China.|Multiple Organ Transplantation Institute of the First Hospital of China Medical University, Shenyang, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Jialin
authors.affiliation: Department of Hepatobiliary Surgery and Organ Transplantation, the First Hospital of China Medical University, Shenyang, China.|National Key Lab. of General Surgery, the First Hospital of China Medical University, Shenyang, China.|Multiple Organ Transplantation Institute of the First Hospital of China Medical University, Shenyang, China.
authors.forename: Li
authors.identifier: http://orcid.org/0000-0001-9008-8162
authors.initials: L
authors.lastname: Baifeng
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D003365:Costs and Cost Analysis; D007515:Islets of Langerhans / Q000166:cytology*; D051379:Mice