title: Oocyte shuttle, a recombinant protein transporting donor DNA into theXenopusoocytein situ.
abstract: The newly developed oocyte shuttle protein contains a streptavidin moiety that tightly binds biotinylated DNA. Injected intravenously into adultXenopusfemales, the protein-DNA complex is rapidly transported through the bloodstream and, within the ovary, the vitellogenin ligand present in the protein binds to the receptors at the surface of the oocytes. The bound complex is internalized and translocates into the oocyte nucleus thanks to an SV40 nuclear localization signal, enhanced by an adjacent casein kinase phosphorylation site. Functioning of the shuttle protein is documented by transporting DNA molecules that, upon intramolecular homologous recombination within the oocyte nucleus, express easily traceable markers such as green fluorescence or tetracycline resistance.
authors.affiliation: Station de Zoologie expérimentale, Department of Genetics and Evolution, University of Geneva, 154 route de Malagnou, Chêne-Bougeries 1224, Switzerland duri.rungger@unige.ch duri.rungger@bluewin.ch.
authors.forename: Duri
authors.identifier: http://orcid.org/0000-0001-6637-5583
authors.initials: D
authors.lastname: Rungger
authors.affiliation: Station de Zoologie expérimentale, Department of Genetics and Evolution, University of Geneva, 154 route de Malagnou, Chêne-Bougeries 1224, Switzerland.
authors.forename: Lisbeth
authors.initials: L
authors.lastname: Muster
authors.affiliation: Institute of Molecular Life Sciences, University of Zurich-Irchel, Winterthurer Strasse 190, Zurich 8057, Switzerland.
authors.forename: Oleg
authors.initials: O
authors.lastname: Georgiev
authors.affiliation: Biologie cellulaire, University Eye Clinic, 20 rue Alcide Jentzer, Geneva 4 1211, Switzerland.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Rungger-Brändle
keywords: Gene delivery; Homologous recombination; Oogenesis; Vitellogenin pathway