title: Involvement of CD147 in alveolar bone remodeling and soft tissue degradation in experimental periodontitis.
abstract: OBJECTIVE
  The objective of this study was to investigate the possible roles of clusters of differentiation 147 (CD147) in bone resorption and mineralization through the bone markers of bone sialoprotein, osteocalcin, osteopontin and alkaline phosphatase (ALP), trabecular structure of alveolar bone and number of osteoclasts. We also investigated the effects of CD147 on inflammation and collagen breakdown.
  
  
  METHODS
  Twenty-eight male Wistar rats were randomly divided into four groups of seven animals each: healthy group, periodontitis group, periodontitis + saline group and periodontitis + anti-CD147 groups. Hematoxylin and eosin staining were used for histological assessment. Alveolar bone loss and trabecula microstructure were evaluated using micro-computed tomography. Collagen fiber breakdown was assessed via picrosirius red staining. Tartrate-resistant acid phosphatase staining was conducted for osteoclast analysis. The expressions of ALP, bone sialoprotein, osteocalcin and osteopontin were evaluated using immunohistochemistry.
  
  
  RESULTS
  Anti-CD147 treatment significantly inhibited alveolar bone loss and osteoclastogenesis, and improved the bone volume/tissue volume, and the trabecular thickness of alveolar bone. Histological staining revealed that anti-CD147 significantly reduced the infiltration of inflammation and limited the fractions of degraded areas in collagen fibers. The expression of bone markers (ALP, bone sialoprotein, osteocalcin and osteopontin) was enhanced by anti-CD147 treatment.
  
  
  CONCLUSIONS
  The results of the anti-CD147 treatment indicate that CD147 was involved in alveolar bone mineralization, osteoclastogenesis and trabecular microstructure. The inhibition of CD147 could increase the expression level of osteogenic markers, alveolar bone crest height and suppressed collagen fiber degradation.
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST KLOS), Key Laboratory for Oral Biomedical Engineering of Ministry of Education (KLOBME), School and Hospital of Stomatology, Wuhan University, Wuhan, China.|Department of Periodontology, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
authors.forename: D
authors.initials: D
authors.lastname: Yang
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST KLOS), Key Laboratory for Oral Biomedical Engineering of Ministry of Education (KLOBME), School and Hospital of Stomatology, Wuhan University, Wuhan, China.
authors.forename: R
authors.initials: R
authors.lastname: Liu
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST KLOS), Key Laboratory for Oral Biomedical Engineering of Ministry of Education (KLOBME), School and Hospital of Stomatology, Wuhan University, Wuhan, China.
authors.forename: L
authors.initials: L
authors.lastname: Liu
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST KLOS), Key Laboratory for Oral Biomedical Engineering of Ministry of Education (KLOBME), School and Hospital of Stomatology, Wuhan University, Wuhan, China.
authors.forename: H
authors.initials: H
authors.lastname: Liao
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST KLOS), Key Laboratory for Oral Biomedical Engineering of Ministry of Education (KLOBME), School and Hospital of Stomatology, Wuhan University, Wuhan, China.
authors.forename: C
authors.initials: C
authors.lastname: Wang
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST KLOS), Key Laboratory for Oral Biomedical Engineering of Ministry of Education (KLOBME), School and Hospital of Stomatology, Wuhan University, Wuhan, China.|Department of Periodontology, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Cao
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D016301:Alveolar Bone Loss / Q000000981:diagnostic imaging / Q000473:pathology*; D000818:Animals; D051926:Basigin / Q000502:physiology*; D015415:Biomarkers / Q000378:metabolism*; D016723:Bone Remodeling / Q000502:physiology*; D003094:Collagen / Q000378:metabolism; D007150:Immunohistochemistry; D058574:Integrin-Binding Sialoprotein / Q000378:metabolism; D008297:Male; D015675:Osteocalcin / Q000378:metabolism; D010010:Osteoclasts / Q000166:cytology; D053495:Osteopontin / Q000378:metabolism; D010518:Periodontitis / Q000473:pathology*; D011897:Random Allocation; D051381:Rats; D055114:X-Ray Microtomography
chemical_list: D015415:Biomarkers; D058574:Integrin-Binding Sialoprotein; D015675:Osteocalcin; D053495:Osteopontin; D051926:Basigin; D003094:Collagen; D000469:Alkaline Phosphatase
keywords: alveolar bone; clusters of differentiation 147; periodontitis; remodeling; resorption