title: Image-based cell quality evaluation to detect irregularities under same culture process of human induced pluripotent stem cells.
abstract: To meet the growing demand for human induced pluripotent stem cells (iPSCs) for various applications, technologies that enable the manufacturing of iPSCs on a large scale should be developed. There are several technological challenges in iPSC manufacturing technology. Image-based cell quality evaluation technology for monitoring iPSC quality in culture enables the manufacture of intact cells for further applications. Although several studies have reported the effectiveness of image-based evaluation of iPSCs, it remains challenging to detect irregularities that may arise using the same processing operations during quality evaluation of automated processing. In this study, we investigated the evaluation performance of image-based cell quality analysis in detecting small differences that can result from human measurement, even when the same protocol is followed. To imitate such culture conditions, by image-analysis guided colony pickup, we changed the proportions of morphologically different subpopulations: "good morphology, regular morphology correlated with undifferentiation marker expression" and "bad morphology, irregular morphology correlated with loss of undifferentiation marker expression". In addition, comprehensive gene-expression and metabolomics analyses were carried out for the same samples to investigate performance differences. Our data shows an example of investigating the usefulness and sensitivity of quality evaluation methods for iPSC quality monitoring.
authors.affiliation: Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Furocho, Chikusa-ku, Nagoya 464-8601, Japan.
authors.forename: Risako
authors.initials: R
authors.lastname: Nagasaka
authors.affiliation: Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Furocho, Chikusa-ku, Nagoya 464-8601, Japan.
authors.forename: Yuto
authors.initials: Y
authors.lastname: Gotou
authors.affiliation: Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Furocho, Chikusa-ku, Nagoya 464-8601, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Yoshida
authors.affiliation: Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Furocho, Chikusa-ku, Nagoya 464-8601, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Kanie
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furocho, Chikusa-ku, Nagoya 464-8602, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Shimizu
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furocho, Chikusa-ku, Nagoya 464-8602, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Honda
authors.affiliation: Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Furocho, Chikusa-ku, Nagoya 464-8601, Japan. Electronic address: kato-r@ps.nagoya-u.ac.jp.
authors.forename: Ryuji
authors.initials: R
authors.lastname: Kato
mesh_terms: D001709:Biotechnology / Q000379:methods* / Q000592:standards*; D002454:Cell Differentiation; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D055432:Metabolomics; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D057054:Molecular Imaging; D011786:Quality Control; D059467:Transcriptome
keywords: Cell quality evaluation; Gene expression profile; Induced pluripotent stem cell; Metabolomic analysis; Morphology analysis; Quality control